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NGEF cDNA ORF clone, Canis lupus familiaris(dog)

Summary

Gene Symbol NGEF
Entrez Gene ID 486165
Full Name neuronal guanine nucleotide exchange factor
General protein information
Preferred Names

neuronal guanine nucleotide exchange factor

Names

ephexin-1
Gene Type protein-coding
Organism Canis lupus familiaris(dog)

Reference Sequences (RefSeq)

mRNA and Protein(s)

mRNA Protein Name
XM_014107552.1 XP_013963027.1 ephexin-1

Pathways from BioSystems


Homologies


Canis lupus familiaris (dog) NGEF XP_005635921.1
Danio rerio (zebrafish) LOC569090 XP_697546.3
Xenopus tropicalis (tropical clawed frog) ngef XP_004917852.1
Bos taurus (cattle) NGEF NP_001070394.1
Rattus norvegicus (Norway rat) Ngef NP_001129713.1
Pan troglodytes (chimpanzee) NGEF XP_516157.3
Homo sapiens (human) NGEF NP_062824.2
LOC713917 XP_001114604.1
Mus musculus (house mouse) Ngef NP_001104784.1
Gallus gallus (chicken) NGEF NP_001010841.1
Anopheles gambiae (African malaria mosquito) AgaP_AGAP007452 XP_308411.4

Gene Ontology

The following NGEF gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the NGEF cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OCe25835 XM_014107552.1
Latest version!		 Canis lupus familiaris neuronal guanine nucleotide exchange factor (NGEF), mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $342.30
$489.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)


CloneID OCe25835
Clone ID Related Accession (Same CDS sequence) XM_014107552.1
Accession Version XM_014107552.1 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 1806bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2017-09-04
Organism Canis lupus familiaris(dog)
Product ephexin-1
Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006607.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
ATGCTTCGGT GGAGAATCCC AAAACAGCCA GACCCAGGAG CCAAGAGAAC AAGCGAGTCG 
TCCTCCACCC CGGGAAATGG GGCCACGCCC GAGGAGTGCC CGGCGCTGGC CGACAGCCCC 
ACCACGCTCA CCGAGGCCCT GCAGATGATC CACCCCATCC CCGCCGACTC CTGGAGAAAC 
CTCATCGAAC AAATAGGGCT CCTGTATCAG GAATACCGAG ATAAATCCAC GCTCCAAGAA 
ATTGAAACCA GGAGGCAGCA GGATGCAGAG ATACACACCA ATGCCCATGG CTCCCCGGCC 
AGCGAGGAGA CCCCAGAAGA GGAGGAGGAC GAGGAGGAGG AGGAGGACGA GCCGGCAAGC 
CCGCCCGAGA GGAAGGCTCT GCCCCAGATC TGCCTCCTCA GTAACCCCCA CTCGAGATTT 
AACCTCTGGC AGGACCTGCC CGAGATCCAG AGCAGCGGGG TGCTGGAAAT CCTCCAGCCC 
GAGGAGATCA AGCTGCAGGA GGCCATGTTC GAGCTGGTCA CTTCTGAGGC CTCCTACTAC 
AAAAGCCTGA GCCTGCTCGT GTCTCACTTC ATGGAGAATG AACGTCTGAA GAAGATCCTG 
CACCCGTCTG AGGCACACAT CCTCTTCTCC AATGTCCTGG ACGTCATGGC CGTCAGTGAA 
CGGTTTCTCC TGGAGCTGGA GCGCCGGATG GAGGAGAACA TCGTCATTTC GGACGTGTGC 
GACATCGTGT ACCATTACGC GGCCAACCAC TTCTCCGTGT ACATCACCTA CGTCAGCAAC 
CAGACCTACC AGGAGAGGAC CTACAAGCAG TTGCTCCAGG AGAAGGCGGC CTTTCGGGAG 
CTGATTGCGC AGTTGGAGCT GGACCCCAAG TGCAGGGGGC TGCCTCTGTC CTCCTTCCTC 
ATCCTGCCTT TCCAGAGGAT CACGCGCCTC AAGCTGTTGA TCCAGAACAT TCTGAAGAGA 
GTGGAAGAAA GGTCTGAGCG CGAGTGCACT GCTTTGGAGG CCCACAAGGA ACTGGAGATG 
GTGGTAAAAG CGTGCAACGA GGGTGTCAGG AAAATGAGCC GCACAGAGCA GATGATCAGC 
ATTCAGAAGA AGATGGAGTT TAAGATCAAG TCCGTGCCCA TCATCTCCCA CTCCCGCTGG 
CTGCTGAAGC AGGGTGAGCT GCAGCAGATG TCAGGCCCCA AGACCTCCCG AACCCTGCGG 
ACCAAGAAAC TCTTCCGGGA AATTTACCTC TTCCTCTTCA ACGACCTGCT GGTGATCTGC 
CGGCAGATCC CCGGAGACAA GTACCAAGTG TTTGACTCGG CCCCGAGGGG CCTCCTCCGG 
GTGGAGGAGC TGGAGGACCA GGGCCAGACG CTGGCCAACG TGTTCATCCT GCGGCTGCTG 
GAGAACGCGG ACGACCGGGA GGCCACCTAC ATGCTGAAGG CGTCTTCTCA GAGTGAGATG 
AAGCGTTGGA TGACCTCACT GGCCCCCAAC CGGAGAACCA AGTTTGTTTC CTTCACTTCC 
CGGCTGCTGG ACTGTCCCCA GGTCCAGTGT GTGCACCCGT ATGTGGCCCA GCAGCCAGAC 
GAGCTGACAC TGGAGCTGGC TGACATTCTG AACATTCTGG AAAAGACCGA AGACGGGTGG 
ATCTTTGGCG AGCGTCTGCA TGACCAGGAG AGAGGCTGGT TCCCCAGCTC CATGACTGAG 
GAGATCTTGA ATCCCAAGAT CCGGTCCCAG AACCTCAAGG AATGTTTCCG AGTCCACAAG 
ATGGACGACC CTCAGCGCAG CCAGAACAAG GATCGCAGGA AGCTGGGCAG CCGAAATCGG 
CAATGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_013963027.1
CDS145..1950
Translation

Target ORF information:

RefSeq Version XM_014107552.1
Organism Canis lupus familiaris(dog)
Definition Canis lupus familiaris neuronal guanine nucleotide exchange factor (NGEF), mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 XM_014107552.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
ATGCTTCGGT GGAGAATCCC AAAACAGCCA GACCCAGGAG CCAAGAGAAC AAGCGAGTCG 
TCCTCCACCC CGGGAAATGG GGCCACGCCC GAGGAGTGCC CGGCGCTGGC CGACAGCCCC 
ACCACGCTCA CCGAGGCCCT GCAGATGATC CACCCCATCC CCGCCGACTC CTGGAGAAAC 
CTCATCGAAC AAATAGGGCT CCTGTATCAG GAATACCGAG ATAAATCCAC GCTCCAAGAA 
ATTGAAACCA GGAGGCAGCA GGATGCAGAG ATACACACCA ATGCCCATGG CTCCCCGGCC 
AGCGAGGAGA CCCCAGAAGA GGAGGAGGAC GAGGAGGAGG AGGAGGACGA GCCGGCAAGC 
CCGCCCGAGA GGAAGGCTCT GCCCCAGATC TGCCTCCTCA GTAACCCCCA CTCGAGATTT 
AACCTCTGGC AGGACCTGCC CGAGATCCAG AGCAGCGGGG TGCTGGAAAT CCTCCAGCCC 
GAGGAGATCA AGCTGCAGGA GGCCATGTTC GAGCTGGTCA CTTCTGAGGC CTCCTACTAC 
AAAAGCCTGA GCCTGCTCGT GTCTCACTTC ATGGAGAATG AACGTCTGAA GAAGATCCTG 
CACCCGTCTG AGGCACACAT CCTCTTCTCC AATGTCCTGG ACGTCATGGC CGTCAGTGAA 
CGGTTTCTCC TGGAGCTGGA GCGCCGGATG GAGGAGAACA TCGTCATTTC GGACGTGTGC 
GACATCGTGT ACCATTACGC GGCCAACCAC TTCTCCGTGT ACATCACCTA CGTCAGCAAC 
CAGACCTACC AGGAGAGGAC CTACAAGCAG TTGCTCCAGG AGAAGGCGGC CTTTCGGGAG 
CTGATTGCGC AGTTGGAGCT GGACCCCAAG TGCAGGGGGC TGCCTCTGTC CTCCTTCCTC 
ATCCTGCCTT TCCAGAGGAT CACGCGCCTC AAGCTGTTGA TCCAGAACAT TCTGAAGAGA 
GTGGAAGAAA GGTCTGAGCG CGAGTGCACT GCTTTGGAGG CCCACAAGGA ACTGGAGATG 
GTGGTAAAAG CGTGCAACGA GGGTGTCAGG AAAATGAGCC GCACAGAGCA GATGATCAGC 
ATTCAGAAGA AGATGGAGTT TAAGATCAAG TCCGTGCCCA TCATCTCCCA CTCCCGCTGG 
CTGCTGAAGC AGGGTGAGCT GCAGCAGATG TCAGGCCCCA AGACCTCCCG AACCCTGCGG 
ACCAAGAAAC TCTTCCGGGA AATTTACCTC TTCCTCTTCA ACGACCTGCT GGTGATCTGC 
CGGCAGATCC CCGGAGACAA GTACCAAGTG TTTGACTCGG CCCCGAGGGG CCTCCTCCGG 
GTGGAGGAGC TGGAGGACCA GGGCCAGACG CTGGCCAACG TGTTCATCCT GCGGCTGCTG 
GAGAACGCGG ACGACCGGGA GGCCACCTAC ATGCTGAAGG CGTCTTCTCA GAGTGAGATG 
AAGCGTTGGA TGACCTCACT GGCCCCCAAC CGGAGAACCA AGTTTGTTTC CTTCACTTCC 
CGGCTGCTGG ACTGTCCCCA GGTCCAGTGT GTGCACCCGT ATGTGGCCCA GCAGCCAGAC 
GAGCTGACAC TGGAGCTGGC TGACATTCTG AACATTCTGG AAAAGACCGA AGACGGGTGG 
ATCTTTGGCG AGCGTCTGCA TGACCAGGAG AGAGGCTGGT TCCCCAGCTC CATGACTGAG 
GAGATCTTGA ATCCCAAGAT CCGGTCCCAG AACCTCAAGG AATGTTTCCG AGTCCACAAG 
ATGGACGACC CTCAGCGCAG CCAGAACAAG GATCGCAGGA AGCTGGGCAG CCGAAATCGG 
CAATGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

PubMed