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MLC1 cDNA ORF clone, Canis lupus familiaris(dog)

Summary

Gene Symbol MLC1
Entrez Gene ID 607105
Full Name megalencephalic leukoencephalopathy with subcortical cysts 1
General protein information
Preferred Names

megalencephalic leukoencephalopathy with subcortical cysts 1

Names

membrane protein MLC1
Gene Type protein-coding
Organism Canis lupus familiaris(dog)

Reference Sequences (RefSeq)

mRNA and Protein(s)

mRNA Protein Name
XM_005625722.2 XP_005625779.1 membrane protein MLC1 isoform X3
XM_005625722.3 XP_005625779.1 membrane protein MLC1 isoform X3
XM_843697.5 XP_848790.2 membrane protein MLC1 isoform X3
XM_022424271.1 XP_022279979.1 membrane protein MLC1 isoform X3
XM_014117268.2 XP_013972743.1 membrane protein MLC1 isoform X1
XM_022424269.1 XP_022279977.1 membrane protein MLC1 isoform X3
XM_014117269.1 XP_013972744.1 membrane protein MLC1 isoform X2
XM_014117268.1 XP_013972743.1 membrane protein MLC1 isoform X1
XM_843697.4 XP_848790.2 membrane protein MLC1 isoform X3
XM_014117269.2 XP_013972744.1 membrane protein MLC1 isoform X2

Pathways from BioSystems


Homologies


MLC1 XP_001111622.1
Mus musculus (house mouse) Mlc1 NP_573504.1
Rattus norvegicus (Norway rat) Mlc1 NP_001101575.1
Danio rerio (zebrafish) mlc1 NP_001098409.1
Homo sapiens (human) MLC1 NP_631941.1
Gallus gallus (chicken) MLC1 XP_004937559.1
Xenopus tropicalis (tropical clawed frog) mlc1 NP_001072921.1
Canis lupus familiaris (dog) MLC1 XP_848790.2
Pan troglodytes (chimpanzee) MLC1 XP_515212.3
Bos taurus (cattle) MLC1 NP_001070420.1

Gene Ontology

The following MLC1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the MLC1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OCe13303 XM_022424271.1
Latest version!		 Canis lupus familiaris megalencephalic leukoencephalopathy with subcortical cysts 1 (MLC1), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $258.30
$369.00
OCe13303 XM_005625722.3
Latest version!		 Canis lupus familiaris megalencephalic leukoencephalopathy with subcortical cysts 1 (MLC1), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $258.30
$369.00
OCe13303 XM_843697.5
Latest version!		 Canis lupus familiaris megalencephalic leukoencephalopathy with subcortical cysts 1 (MLC1), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $258.30
$369.00
OCe13303 XM_022424269.1
Latest version!		 Canis lupus familiaris megalencephalic leukoencephalopathy with subcortical cysts 1 (MLC1), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $258.30
$369.00
OCe38848 XM_014117268.2
Latest version!		 Canis lupus familiaris megalencephalic leukoencephalopathy with subcortical cysts 1 (MLC1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 9-11 $258.30
$369.00
OCe38849 XM_014117269.2
Latest version!		 Canis lupus familiaris megalencephalic leukoencephalopathy with subcortical cysts 1 (MLC1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 9-11 $258.30
$369.00
View Old Accession Versions >>
OCe13303 XM_005625722.2 Canis lupus familiaris megalencephalic leukoencephalopathy with subcortical cysts 1 (MLC1), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $258.30
$369.00
OCe38848 XM_014117268.1 Canis lupus familiaris megalencephalic leukoencephalopathy with subcortical cysts 1 (MLC1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 9-11 $258.30
$369.00
OCe13303 XM_843697.4 Canis lupus familiaris megalencephalic leukoencephalopathy with subcortical cysts 1 (MLC1), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $258.30
$369.00
OCe38849 XM_014117269.1 Canis lupus familiaris megalencephalic leukoencephalopathy with subcortical cysts 1 (MLC1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 9-11 $258.30
$369.00
Hide Old Accession Versions >>

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)


CloneID OCe13303
Clone ID Related Accession (Same CDS sequence) XM_005625722.2 , XM_005625722.3 , XM_843697.5 , XM_022424271.1 , XM_022424269.1 , XM_843697.4
Accession Version XM_005625722.2 Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 1122bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2015-09-16
Organism Canis lupus familiaris(Dog)
Product membrane protein MLC1 isoform X3
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726078.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_005625722.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
ATGTCGCGGG ACGAGCAGGG CAGGGAGGAG CTCGGTTACG ATCGCATGCC CACCCTGGAG 
CGCGGAAGGC CTGACGCAGG GAGCTACGCC CTGGACACCA AGCCCAATGA CCTCCAGCTG 
GCGACGAGGC TGCCCCCATG CTTCAGTCAC AAGACGTGGG TCCTCTCCGT GTTGATGGGG 
AGCTGTCTAC TTGTGACGTC GGGGTTTTCA CTCTACCTGG GAAATGTGTT TCCCTCTGAA 
ATGGATTACT TACGTTGTGC TGCGGGATCG TGCCTTCCCT CAGCAATCGT GAGCTTTGCC 
GTTTCACGGA GAAACATCAG TGCGATCCCC AACTTTCAGA TCCTGTTTGT GTCCACATTC 
GCAATCACCA CTACATGTTT AATCTGGTTT GGATGCAAAC TGGTCGTGAA CCCCTCGGCA 
ATAGACATAA ATTTTAATCT GATTCTGCTC CTTCTGTTGG AGCTTTTCAT GGCGGCCACA 
GTGATCATCT CAGCACGATC AAGTGAGGCG CACTGTAAGC AGAAGGGTCC CATGTTGGAG 
AGCGCCAACA TTTTGTATGA AGTGGCATTT CCTGCCCGGG TCCTAAAATC CTATTCAGTC 
ATCGAGGTGA TTGCTGGTGT CTCTGCTGTC CTGGGTGGGG TTATCGCTCT GAACGTGGAT 
GACGCTGTCT CGGGGCCACA CCTCTCAGTG ACGTTCTTTT GGATCTTAGT GGCCTGTTTT 
CCAAGTGCCA TTGCCAGTCA CGTCACAGCC GAGTGTCCCA GCAAGTGTCT GGTGGAGGTG 
CTGATCGCCA TCTGCAGCCT CACGTCCCCG CTGCTGTTCA CGGCCTCTGG GTACCTGTCA 
TTCAGCATCA TGAGAATCAT GGAGATCTTC AAAGATTACC CACCGGCCAT CAAGCAATCC 
TATGACGTGC TCCTGCTGCT TCTCATGCTG GAGCTGCTGC TGCAGGCCGG CCTCAACACG 
GGCACCGTCA TCCAGTGTGT GAGCTTCAAG CTGGGAGCAT CCTGGGATGC CGCACAGACC 
AGCCAGCAGG AGCGCCCCCC AGGGGAGGTG TCCAGGAGCC CACTGAAGGA GTTCGACAAG 
GAGAAGGCGT GGAGGGCCGT GGTGGTACAG ATGGCCCAGT GA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_005625779.1
CDS431..1552
Translation

Target ORF information:

RefSeq Version XM_005625722.2
Organism Canis lupus familiaris(Dog)
Definition Canis lupus familiaris megalencephalic leukoencephalopathy with subcortical cysts 1 (MLC1), transcript variant X4, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 XM_005625722.2

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
ATGTCGCGGG ACGAGCAGGG CAGGGAGGAG CTCGGTTACG ATCGCATGCC CACCCTGGAG 
CGCGGAAGGC CTGACGCAGG GAGCTACGCC CTGGACACCA AGCCCAATGA CCTCCAGCTG 
GCGACGAGGC TGCCCCCATG CTTCAGTCAC AAGACGTGGG TCCTCTCCGT GTTGATGGGG 
AGCTGTCTAC TTGTGACGTC GGGGTTTTCA CTCTACCTGG GAAATGTGTT TCCCTCTGAA 
ATGGATTACT TACGTTGTGC TGCGGGATCG TGCCTTCCCT CAGCAATCGT GAGCTTTGCC 
GTTTCACGGA GAAACATCAG TGCGATCCCC AACTTTCAGA TCCTGTTTGT GTCCACATTC 
GCAATCACCA CTACATGTTT AATCTGGTTT GGATGCAAAC TGGTCGTGAA CCCCTCGGCA 
ATAGACATAA ATTTTAATCT GATTCTGCTC CTTCTGTTGG AGCTTTTCAT GGCGGCCACA 
GTGATCATCT CAGCACGATC AAGTGAGGCG CACTGTAAGC AGAAGGGTCC CATGTTGGAG 
AGCGCCAACA TTTTGTATGA AGTGGCATTT CCTGCCCGGG TCCTAAAATC CTATTCAGTC 
ATCGAGGTGA TTGCTGGTGT CTCTGCTGTC CTGGGTGGGG TTATCGCTCT GAACGTGGAT 
GACGCTGTCT CGGGGCCACA CCTCTCAGTG ACGTTCTTTT GGATCTTAGT GGCCTGTTTT 
CCAAGTGCCA TTGCCAGTCA CGTCACAGCC GAGTGTCCCA GCAAGTGTCT GGTGGAGGTG 
CTGATCGCCA TCTGCAGCCT CACGTCCCCG CTGCTGTTCA CGGCCTCTGG GTACCTGTCA 
TTCAGCATCA TGAGAATCAT GGAGATCTTC AAAGATTACC CACCGGCCAT CAAGCAATCC 
TATGACGTGC TCCTGCTGCT TCTCATGCTG GAGCTGCTGC TGCAGGCCGG CCTCAACACG 
GGCACCGTCA TCCAGTGTGT GAGCTTCAAG CTGGGAGCAT CCTGGGATGC CGCACAGACC 
AGCCAGCAGG AGCGCCCCCC AGGGGAGGTG TCCAGGAGCC CACTGAAGGA GTTCGACAAG 
GAGAAGGCGT GGAGGGCCGT GGTGGTACAG ATGGCCCAGT GA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OCe13303
Clone ID Related Accession (Same CDS sequence) XM_005625722.2 , XM_005625722.3 , XM_843697.5 , XM_022424271.1 , XM_022424269.1 , XM_843697.4
Accession Version XM_005625722.3 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 1122bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2017-09-04
Organism Canis lupus familiaris(dog)
Product membrane protein MLC1 isoform X3
Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006592.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_005625722.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
ATGTCGCGGG ACGAGCAGGG CAGGGAGGAG CTCGGTTACG ATCGCATGCC CACCCTGGAG 
CGCGGAAGGC CTGACGCAGG GAGCTACGCC CTGGACACCA AGCCCAATGA CCTCCAGCTG 
GCGACGAGGC TGCCCCCATG CTTCAGTCAC AAGACGTGGG TCCTCTCCGT GTTGATGGGG 
AGCTGTCTAC TTGTGACGTC GGGGTTTTCA CTCTACCTGG GAAATGTGTT TCCCTCTGAA 
ATGGATTACT TACGTTGTGC TGCGGGATCG TGCCTTCCCT CAGCAATCGT GAGCTTTGCC 
GTTTCACGGA GAAACATCAG TGCGATCCCC AACTTTCAGA TCCTGTTTGT GTCCACATTC 
GCAATCACCA CTACATGTTT AATCTGGTTT GGATGCAAAC TGGTCGTGAA CCCCTCGGCA 
ATAGACATAA ATTTTAATCT GATTCTGCTC CTTCTGTTGG AGCTTTTCAT GGCGGCCACA 
GTGATCATCT CAGCACGATC AAGTGAGGCG CACTGTAAGC AGAAGGGTCC CATGTTGGAG 
AGCGCCAACA TTTTGTATGA AGTGGCATTT CCTGCCCGGG TCCTAAAATC CTATTCAGTC 
ATCGAGGTGA TTGCTGGTGT CTCTGCTGTC CTGGGTGGGG TTATCGCTCT GAACGTGGAT 
GACGCTGTCT CGGGGCCACA CCTCTCAGTG ACGTTCTTTT GGATCTTAGT GGCCTGTTTT 
CCAAGTGCCA TTGCCAGTCA CGTCACAGCC GAGTGTCCCA GCAAGTGTCT GGTGGAGGTG 
CTGATCGCCA TCTGCAGCCT CACGTCCCCG CTGCTGTTCA CGGCCTCTGG GTACCTGTCA 
TTCAGCATCA TGAGAATCAT GGAGATCTTC AAAGATTACC CACCGGCCAT CAAGCAATCC 
TATGACGTGC TCCTGCTGCT TCTCATGCTG GAGCTGCTGC TGCAGGCCGG CCTCAACACG 
GGCACCGTCA TCCAGTGTGT GAGCTTCAAG CTGGGAGCAT CCTGGGATGC CGCACAGACC 
AGCCAGCAGG AGCGCCCCCC AGGGGAGGTG TCCAGGAGCC CACTGAAGGA GTTCGACAAG 
GAGAAGGCGT GGAGGGCCGT GGTGGTACAG ATGGCCCAGT GA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_005625779.1
CDS336..1457
Translation

Target ORF information:

RefSeq Version XM_005625722.3
Organism Canis lupus familiaris(dog)
Definition Canis lupus familiaris megalencephalic leukoencephalopathy with subcortical cysts 1 (MLC1), transcript variant X4, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 XM_005625722.3

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
ATGTCGCGGG ACGAGCAGGG CAGGGAGGAG CTCGGTTACG ATCGCATGCC CACCCTGGAG 
CGCGGAAGGC CTGACGCAGG GAGCTACGCC CTGGACACCA AGCCCAATGA CCTCCAGCTG 
GCGACGAGGC TGCCCCCATG CTTCAGTCAC AAGACGTGGG TCCTCTCCGT GTTGATGGGG 
AGCTGTCTAC TTGTGACGTC GGGGTTTTCA CTCTACCTGG GAAATGTGTT TCCCTCTGAA 
ATGGATTACT TACGTTGTGC TGCGGGATCG TGCCTTCCCT CAGCAATCGT GAGCTTTGCC 
GTTTCACGGA GAAACATCAG TGCGATCCCC AACTTTCAGA TCCTGTTTGT GTCCACATTC 
GCAATCACCA CTACATGTTT AATCTGGTTT GGATGCAAAC TGGTCGTGAA CCCCTCGGCA 
ATAGACATAA ATTTTAATCT GATTCTGCTC CTTCTGTTGG AGCTTTTCAT GGCGGCCACA 
GTGATCATCT CAGCACGATC AAGTGAGGCG CACTGTAAGC AGAAGGGTCC CATGTTGGAG 
AGCGCCAACA TTTTGTATGA AGTGGCATTT CCTGCCCGGG TCCTAAAATC CTATTCAGTC 
ATCGAGGTGA TTGCTGGTGT CTCTGCTGTC CTGGGTGGGG TTATCGCTCT GAACGTGGAT 
GACGCTGTCT CGGGGCCACA CCTCTCAGTG ACGTTCTTTT GGATCTTAGT GGCCTGTTTT 
CCAAGTGCCA TTGCCAGTCA CGTCACAGCC GAGTGTCCCA GCAAGTGTCT GGTGGAGGTG 
CTGATCGCCA TCTGCAGCCT CACGTCCCCG CTGCTGTTCA CGGCCTCTGG GTACCTGTCA 
TTCAGCATCA TGAGAATCAT GGAGATCTTC AAAGATTACC CACCGGCCAT CAAGCAATCC 
TATGACGTGC TCCTGCTGCT TCTCATGCTG GAGCTGCTGC TGCAGGCCGG CCTCAACACG 
GGCACCGTCA TCCAGTGTGT GAGCTTCAAG CTGGGAGCAT CCTGGGATGC CGCACAGACC 
AGCCAGCAGG AGCGCCCCCC AGGGGAGGTG TCCAGGAGCC CACTGAAGGA GTTCGACAAG 
GAGAAGGCGT GGAGGGCCGT GGTGGTACAG ATGGCCCAGT GA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OCe13303
Clone ID Related Accession (Same CDS sequence) XM_005625722.2 , XM_005625722.3 , XM_843697.5 , XM_022424271.1 , XM_022424269.1 , XM_843697.4
Accession Version XM_843697.5 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 1122bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2017-09-04
Organism Canis lupus familiaris(dog)
Product membrane protein MLC1 isoform X3
Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006592.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_843697.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
ATGTCGCGGG ACGAGCAGGG CAGGGAGGAG CTCGGTTACG ATCGCATGCC CACCCTGGAG 
CGCGGAAGGC CTGACGCAGG GAGCTACGCC CTGGACACCA AGCCCAATGA CCTCCAGCTG 
GCGACGAGGC TGCCCCCATG CTTCAGTCAC AAGACGTGGG TCCTCTCCGT GTTGATGGGG 
AGCTGTCTAC TTGTGACGTC GGGGTTTTCA CTCTACCTGG GAAATGTGTT TCCCTCTGAA 
ATGGATTACT TACGTTGTGC TGCGGGATCG TGCCTTCCCT CAGCAATCGT GAGCTTTGCC 
GTTTCACGGA GAAACATCAG TGCGATCCCC AACTTTCAGA TCCTGTTTGT GTCCACATTC 
GCAATCACCA CTACATGTTT AATCTGGTTT GGATGCAAAC TGGTCGTGAA CCCCTCGGCA 
ATAGACATAA ATTTTAATCT GATTCTGCTC CTTCTGTTGG AGCTTTTCAT GGCGGCCACA 
GTGATCATCT CAGCACGATC AAGTGAGGCG CACTGTAAGC AGAAGGGTCC CATGTTGGAG 
AGCGCCAACA TTTTGTATGA AGTGGCATTT CCTGCCCGGG TCCTAAAATC CTATTCAGTC 
ATCGAGGTGA TTGCTGGTGT CTCTGCTGTC CTGGGTGGGG TTATCGCTCT GAACGTGGAT 
GACGCTGTCT CGGGGCCACA CCTCTCAGTG ACGTTCTTTT GGATCTTAGT GGCCTGTTTT 
CCAAGTGCCA TTGCCAGTCA CGTCACAGCC GAGTGTCCCA GCAAGTGTCT GGTGGAGGTG 
CTGATCGCCA TCTGCAGCCT CACGTCCCCG CTGCTGTTCA CGGCCTCTGG GTACCTGTCA 
TTCAGCATCA TGAGAATCAT GGAGATCTTC AAAGATTACC CACCGGCCAT CAAGCAATCC 
TATGACGTGC TCCTGCTGCT TCTCATGCTG GAGCTGCTGC TGCAGGCCGG CCTCAACACG 
GGCACCGTCA TCCAGTGTGT GAGCTTCAAG CTGGGAGCAT CCTGGGATGC CGCACAGACC 
AGCCAGCAGG AGCGCCCCCC AGGGGAGGTG TCCAGGAGCC CACTGAAGGA GTTCGACAAG 
GAGAAGGCGT GGAGGGCCGT GGTGGTACAG ATGGCCCAGT GA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_848790.2
CDS183..1304
Translation

Target ORF information:

RefSeq Version XM_843697.5
Organism Canis lupus familiaris(dog)
Definition Canis lupus familiaris megalencephalic leukoencephalopathy with subcortical cysts 1 (MLC1), transcript variant X5, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 XM_843697.5

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
ATGTCGCGGG ACGAGCAGGG CAGGGAGGAG CTCGGTTACG ATCGCATGCC CACCCTGGAG 
CGCGGAAGGC CTGACGCAGG GAGCTACGCC CTGGACACCA AGCCCAATGA CCTCCAGCTG 
GCGACGAGGC TGCCCCCATG CTTCAGTCAC AAGACGTGGG TCCTCTCCGT GTTGATGGGG 
AGCTGTCTAC TTGTGACGTC GGGGTTTTCA CTCTACCTGG GAAATGTGTT TCCCTCTGAA 
ATGGATTACT TACGTTGTGC TGCGGGATCG TGCCTTCCCT CAGCAATCGT GAGCTTTGCC 
GTTTCACGGA GAAACATCAG TGCGATCCCC AACTTTCAGA TCCTGTTTGT GTCCACATTC 
GCAATCACCA CTACATGTTT AATCTGGTTT GGATGCAAAC TGGTCGTGAA CCCCTCGGCA 
ATAGACATAA ATTTTAATCT GATTCTGCTC CTTCTGTTGG AGCTTTTCAT GGCGGCCACA 
GTGATCATCT CAGCACGATC AAGTGAGGCG CACTGTAAGC AGAAGGGTCC CATGTTGGAG 
AGCGCCAACA TTTTGTATGA AGTGGCATTT CCTGCCCGGG TCCTAAAATC CTATTCAGTC 
ATCGAGGTGA TTGCTGGTGT CTCTGCTGTC CTGGGTGGGG TTATCGCTCT GAACGTGGAT 
GACGCTGTCT CGGGGCCACA CCTCTCAGTG ACGTTCTTTT GGATCTTAGT GGCCTGTTTT 
CCAAGTGCCA TTGCCAGTCA CGTCACAGCC GAGTGTCCCA GCAAGTGTCT GGTGGAGGTG 
CTGATCGCCA TCTGCAGCCT CACGTCCCCG CTGCTGTTCA CGGCCTCTGG GTACCTGTCA 
TTCAGCATCA TGAGAATCAT GGAGATCTTC AAAGATTACC CACCGGCCAT CAAGCAATCC 
TATGACGTGC TCCTGCTGCT TCTCATGCTG GAGCTGCTGC TGCAGGCCGG CCTCAACACG 
GGCACCGTCA TCCAGTGTGT GAGCTTCAAG CTGGGAGCAT CCTGGGATGC CGCACAGACC 
AGCCAGCAGG AGCGCCCCCC AGGGGAGGTG TCCAGGAGCC CACTGAAGGA GTTCGACAAG 
GAGAAGGCGT GGAGGGCCGT GGTGGTACAG ATGGCCCAGT GA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OCe13303
Clone ID Related Accession (Same CDS sequence) XM_005625722.2 , XM_005625722.3 , XM_843697.5 , XM_022424271.1 , XM_022424269.1 , XM_843697.4
Accession Version XM_022424271.1 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 1122bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2017-09-04
Organism Canis lupus familiaris(dog)
Product membrane protein MLC1 isoform X3
Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006592.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
ATGTCGCGGG ACGAGCAGGG CAGGGAGGAG CTCGGTTACG ATCGCATGCC CACCCTGGAG 
CGCGGAAGGC CTGACGCAGG GAGCTACGCC CTGGACACCA AGCCCAATGA CCTCCAGCTG 
GCGACGAGGC TGCCCCCATG CTTCAGTCAC AAGACGTGGG TCCTCTCCGT GTTGATGGGG 
AGCTGTCTAC TTGTGACGTC GGGGTTTTCA CTCTACCTGG GAAATGTGTT TCCCTCTGAA 
ATGGATTACT TACGTTGTGC TGCGGGATCG TGCCTTCCCT CAGCAATCGT GAGCTTTGCC 
GTTTCACGGA GAAACATCAG TGCGATCCCC AACTTTCAGA TCCTGTTTGT GTCCACATTC 
GCAATCACCA CTACATGTTT AATCTGGTTT GGATGCAAAC TGGTCGTGAA CCCCTCGGCA 
ATAGACATAA ATTTTAATCT GATTCTGCTC CTTCTGTTGG AGCTTTTCAT GGCGGCCACA 
GTGATCATCT CAGCACGATC AAGTGAGGCG CACTGTAAGC AGAAGGGTCC CATGTTGGAG 
AGCGCCAACA TTTTGTATGA AGTGGCATTT CCTGCCCGGG TCCTAAAATC CTATTCAGTC 
ATCGAGGTGA TTGCTGGTGT CTCTGCTGTC CTGGGTGGGG TTATCGCTCT GAACGTGGAT 
GACGCTGTCT CGGGGCCACA CCTCTCAGTG ACGTTCTTTT GGATCTTAGT GGCCTGTTTT 
CCAAGTGCCA TTGCCAGTCA CGTCACAGCC GAGTGTCCCA GCAAGTGTCT GGTGGAGGTG 
CTGATCGCCA TCTGCAGCCT CACGTCCCCG CTGCTGTTCA CGGCCTCTGG GTACCTGTCA 
TTCAGCATCA TGAGAATCAT GGAGATCTTC AAAGATTACC CACCGGCCAT CAAGCAATCC 
TATGACGTGC TCCTGCTGCT TCTCATGCTG GAGCTGCTGC TGCAGGCCGG CCTCAACACG 
GGCACCGTCA TCCAGTGTGT GAGCTTCAAG CTGGGAGCAT CCTGGGATGC CGCACAGACC 
AGCCAGCAGG AGCGCCCCCC AGGGGAGGTG TCCAGGAGCC CACTGAAGGA GTTCGACAAG 
GAGAAGGCGT GGAGGGCCGT GGTGGTACAG ATGGCCCAGT GA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_022279979.1
CDS552..1673
Translation

Target ORF information:

RefSeq Version XM_022424271.1
Organism Canis lupus familiaris(dog)
Definition Canis lupus familiaris megalencephalic leukoencephalopathy with subcortical cysts 1 (MLC1), transcript variant X6, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 XM_022424271.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
ATGTCGCGGG ACGAGCAGGG CAGGGAGGAG CTCGGTTACG ATCGCATGCC CACCCTGGAG 
CGCGGAAGGC CTGACGCAGG GAGCTACGCC CTGGACACCA AGCCCAATGA CCTCCAGCTG 
GCGACGAGGC TGCCCCCATG CTTCAGTCAC AAGACGTGGG TCCTCTCCGT GTTGATGGGG 
AGCTGTCTAC TTGTGACGTC GGGGTTTTCA CTCTACCTGG GAAATGTGTT TCCCTCTGAA 
ATGGATTACT TACGTTGTGC TGCGGGATCG TGCCTTCCCT CAGCAATCGT GAGCTTTGCC 
GTTTCACGGA GAAACATCAG TGCGATCCCC AACTTTCAGA TCCTGTTTGT GTCCACATTC 
GCAATCACCA CTACATGTTT AATCTGGTTT GGATGCAAAC TGGTCGTGAA CCCCTCGGCA 
ATAGACATAA ATTTTAATCT GATTCTGCTC CTTCTGTTGG AGCTTTTCAT GGCGGCCACA 
GTGATCATCT CAGCACGATC AAGTGAGGCG CACTGTAAGC AGAAGGGTCC CATGTTGGAG 
AGCGCCAACA TTTTGTATGA AGTGGCATTT CCTGCCCGGG TCCTAAAATC CTATTCAGTC 
ATCGAGGTGA TTGCTGGTGT CTCTGCTGTC CTGGGTGGGG TTATCGCTCT GAACGTGGAT 
GACGCTGTCT CGGGGCCACA CCTCTCAGTG ACGTTCTTTT GGATCTTAGT GGCCTGTTTT 
CCAAGTGCCA TTGCCAGTCA CGTCACAGCC GAGTGTCCCA GCAAGTGTCT GGTGGAGGTG 
CTGATCGCCA TCTGCAGCCT CACGTCCCCG CTGCTGTTCA CGGCCTCTGG GTACCTGTCA 
TTCAGCATCA TGAGAATCAT GGAGATCTTC AAAGATTACC CACCGGCCAT CAAGCAATCC 
TATGACGTGC TCCTGCTGCT TCTCATGCTG GAGCTGCTGC TGCAGGCCGG CCTCAACACG 
GGCACCGTCA TCCAGTGTGT GAGCTTCAAG CTGGGAGCAT CCTGGGATGC CGCACAGACC 
AGCCAGCAGG AGCGCCCCCC AGGGGAGGTG TCCAGGAGCC CACTGAAGGA GTTCGACAAG 
GAGAAGGCGT GGAGGGCCGT GGTGGTACAG ATGGCCCAGT GA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OCe13303
Clone ID Related Accession (Same CDS sequence) XM_005625722.2 , XM_005625722.3 , XM_843697.5 , XM_022424271.1 , XM_022424269.1 , XM_843697.4
Accession Version XM_022424269.1 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 1122bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2017-09-04
Organism Canis lupus familiaris(dog)
Product membrane protein MLC1 isoform X3
Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006592.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
ATGTCGCGGG ACGAGCAGGG CAGGGAGGAG CTCGGTTACG ATCGCATGCC CACCCTGGAG 
CGCGGAAGGC CTGACGCAGG GAGCTACGCC CTGGACACCA AGCCCAATGA CCTCCAGCTG 
GCGACGAGGC TGCCCCCATG CTTCAGTCAC AAGACGTGGG TCCTCTCCGT GTTGATGGGG 
AGCTGTCTAC TTGTGACGTC GGGGTTTTCA CTCTACCTGG GAAATGTGTT TCCCTCTGAA 
ATGGATTACT TACGTTGTGC TGCGGGATCG TGCCTTCCCT CAGCAATCGT GAGCTTTGCC 
GTTTCACGGA GAAACATCAG TGCGATCCCC AACTTTCAGA TCCTGTTTGT GTCCACATTC 
GCAATCACCA CTACATGTTT AATCTGGTTT GGATGCAAAC TGGTCGTGAA CCCCTCGGCA 
ATAGACATAA ATTTTAATCT GATTCTGCTC CTTCTGTTGG AGCTTTTCAT GGCGGCCACA 
GTGATCATCT CAGCACGATC AAGTGAGGCG CACTGTAAGC AGAAGGGTCC CATGTTGGAG 
AGCGCCAACA TTTTGTATGA AGTGGCATTT CCTGCCCGGG TCCTAAAATC CTATTCAGTC 
ATCGAGGTGA TTGCTGGTGT CTCTGCTGTC CTGGGTGGGG TTATCGCTCT GAACGTGGAT 
GACGCTGTCT CGGGGCCACA CCTCTCAGTG ACGTTCTTTT GGATCTTAGT GGCCTGTTTT 
CCAAGTGCCA TTGCCAGTCA CGTCACAGCC GAGTGTCCCA GCAAGTGTCT GGTGGAGGTG 
CTGATCGCCA TCTGCAGCCT CACGTCCCCG CTGCTGTTCA CGGCCTCTGG GTACCTGTCA 
TTCAGCATCA TGAGAATCAT GGAGATCTTC AAAGATTACC CACCGGCCAT CAAGCAATCC 
TATGACGTGC TCCTGCTGCT TCTCATGCTG GAGCTGCTGC TGCAGGCCGG CCTCAACACG 
GGCACCGTCA TCCAGTGTGT GAGCTTCAAG CTGGGAGCAT CCTGGGATGC CGCACAGACC 
AGCCAGCAGG AGCGCCCCCC AGGGGAGGTG TCCAGGAGCC CACTGAAGGA GTTCGACAAG 
GAGAAGGCGT GGAGGGCCGT GGTGGTACAG ATGGCCCAGT GA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_022279977.1
CDS317..1438
Translation

Target ORF information:

RefSeq Version XM_022424269.1
Organism Canis lupus familiaris(dog)
Definition Canis lupus familiaris megalencephalic leukoencephalopathy with subcortical cysts 1 (MLC1), transcript variant X3, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 XM_022424269.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
ATGTCGCGGG ACGAGCAGGG CAGGGAGGAG CTCGGTTACG ATCGCATGCC CACCCTGGAG 
CGCGGAAGGC CTGACGCAGG GAGCTACGCC CTGGACACCA AGCCCAATGA CCTCCAGCTG 
GCGACGAGGC TGCCCCCATG CTTCAGTCAC AAGACGTGGG TCCTCTCCGT GTTGATGGGG 
AGCTGTCTAC TTGTGACGTC GGGGTTTTCA CTCTACCTGG GAAATGTGTT TCCCTCTGAA 
ATGGATTACT TACGTTGTGC TGCGGGATCG TGCCTTCCCT CAGCAATCGT GAGCTTTGCC 
GTTTCACGGA GAAACATCAG TGCGATCCCC AACTTTCAGA TCCTGTTTGT GTCCACATTC 
GCAATCACCA CTACATGTTT AATCTGGTTT GGATGCAAAC TGGTCGTGAA CCCCTCGGCA 
ATAGACATAA ATTTTAATCT GATTCTGCTC CTTCTGTTGG AGCTTTTCAT GGCGGCCACA 
GTGATCATCT CAGCACGATC AAGTGAGGCG CACTGTAAGC AGAAGGGTCC CATGTTGGAG 
AGCGCCAACA TTTTGTATGA AGTGGCATTT CCTGCCCGGG TCCTAAAATC CTATTCAGTC 
ATCGAGGTGA TTGCTGGTGT CTCTGCTGTC CTGGGTGGGG TTATCGCTCT GAACGTGGAT 
GACGCTGTCT CGGGGCCACA CCTCTCAGTG ACGTTCTTTT GGATCTTAGT GGCCTGTTTT 
CCAAGTGCCA TTGCCAGTCA CGTCACAGCC GAGTGTCCCA GCAAGTGTCT GGTGGAGGTG 
CTGATCGCCA TCTGCAGCCT CACGTCCCCG CTGCTGTTCA CGGCCTCTGG GTACCTGTCA 
TTCAGCATCA TGAGAATCAT GGAGATCTTC AAAGATTACC CACCGGCCAT CAAGCAATCC 
TATGACGTGC TCCTGCTGCT TCTCATGCTG GAGCTGCTGC TGCAGGCCGG CCTCAACACG 
GGCACCGTCA TCCAGTGTGT GAGCTTCAAG CTGGGAGCAT CCTGGGATGC CGCACAGACC 
AGCCAGCAGG AGCGCCCCCC AGGGGAGGTG TCCAGGAGCC CACTGAAGGA GTTCGACAAG 
GAGAAGGCGT GGAGGGCCGT GGTGGTACAG ATGGCCCAGT GA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OCe13303
Clone ID Related Accession (Same CDS sequence) XM_005625722.2 , XM_005625722.3 , XM_843697.5 , XM_022424271.1 , XM_022424269.1 , XM_843697.4
Accession Version XM_843697.4 Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 1122bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2015-09-16
Organism Canis lupus familiaris(Dog)
Product membrane protein MLC1 isoform X3
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726078.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_843697.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
ATGTCGCGGG ACGAGCAGGG CAGGGAGGAG CTCGGTTACG ATCGCATGCC CACCCTGGAG 
CGCGGAAGGC CTGACGCAGG GAGCTACGCC CTGGACACCA AGCCCAATGA CCTCCAGCTG 
GCGACGAGGC TGCCCCCATG CTTCAGTCAC AAGACGTGGG TCCTCTCCGT GTTGATGGGG 
AGCTGTCTAC TTGTGACGTC GGGGTTTTCA CTCTACCTGG GAAATGTGTT TCCCTCTGAA 
ATGGATTACT TACGTTGTGC TGCGGGATCG TGCCTTCCCT CAGCAATCGT GAGCTTTGCC 
GTTTCACGGA GAAACATCAG TGCGATCCCC AACTTTCAGA TCCTGTTTGT GTCCACATTC 
GCAATCACCA CTACATGTTT AATCTGGTTT GGATGCAAAC TGGTCGTGAA CCCCTCGGCA 
ATAGACATAA ATTTTAATCT GATTCTGCTC CTTCTGTTGG AGCTTTTCAT GGCGGCCACA 
GTGATCATCT CAGCACGATC AAGTGAGGCG CACTGTAAGC AGAAGGGTCC CATGTTGGAG 
AGCGCCAACA TTTTGTATGA AGTGGCATTT CCTGCCCGGG TCCTAAAATC CTATTCAGTC 
ATCGAGGTGA TTGCTGGTGT CTCTGCTGTC CTGGGTGGGG TTATCGCTCT GAACGTGGAT 
GACGCTGTCT CGGGGCCACA CCTCTCAGTG ACGTTCTTTT GGATCTTAGT GGCCTGTTTT 
CCAAGTGCCA TTGCCAGTCA CGTCACAGCC GAGTGTCCCA GCAAGTGTCT GGTGGAGGTG 
CTGATCGCCA TCTGCAGCCT CACGTCCCCG CTGCTGTTCA CGGCCTCTGG GTACCTGTCA 
TTCAGCATCA TGAGAATCAT GGAGATCTTC AAAGATTACC CACCGGCCAT CAAGCAATCC 
TATGACGTGC TCCTGCTGCT TCTCATGCTG GAGCTGCTGC TGCAGGCCGG CCTCAACACG 
GGCACCGTCA TCCAGTGTGT GAGCTTCAAG CTGGGAGCAT CCTGGGATGC CGCACAGACC 
AGCCAGCAGG AGCGCCCCCC AGGGGAGGTG TCCAGGAGCC CACTGAAGGA GTTCGACAAG 
GAGAAGGCGT GGAGGGCCGT GGTGGTACAG ATGGCCCAGT GA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_848790.2
CDS170..1291
Translation

Target ORF information:

RefSeq Version XM_843697.4
Organism Canis lupus familiaris(Dog)
Definition Canis lupus familiaris megalencephalic leukoencephalopathy with subcortical cysts 1 (MLC1), transcript variant X3, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 XM_843697.4

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
ATGTCGCGGG ACGAGCAGGG CAGGGAGGAG CTCGGTTACG ATCGCATGCC CACCCTGGAG 
CGCGGAAGGC CTGACGCAGG GAGCTACGCC CTGGACACCA AGCCCAATGA CCTCCAGCTG 
GCGACGAGGC TGCCCCCATG CTTCAGTCAC AAGACGTGGG TCCTCTCCGT GTTGATGGGG 
AGCTGTCTAC TTGTGACGTC GGGGTTTTCA CTCTACCTGG GAAATGTGTT TCCCTCTGAA 
ATGGATTACT TACGTTGTGC TGCGGGATCG TGCCTTCCCT CAGCAATCGT GAGCTTTGCC 
GTTTCACGGA GAAACATCAG TGCGATCCCC AACTTTCAGA TCCTGTTTGT GTCCACATTC 
GCAATCACCA CTACATGTTT AATCTGGTTT GGATGCAAAC TGGTCGTGAA CCCCTCGGCA 
ATAGACATAA ATTTTAATCT GATTCTGCTC CTTCTGTTGG AGCTTTTCAT GGCGGCCACA 
GTGATCATCT CAGCACGATC AAGTGAGGCG CACTGTAAGC AGAAGGGTCC CATGTTGGAG 
AGCGCCAACA TTTTGTATGA AGTGGCATTT CCTGCCCGGG TCCTAAAATC CTATTCAGTC 
ATCGAGGTGA TTGCTGGTGT CTCTGCTGTC CTGGGTGGGG TTATCGCTCT GAACGTGGAT 
GACGCTGTCT CGGGGCCACA CCTCTCAGTG ACGTTCTTTT GGATCTTAGT GGCCTGTTTT 
CCAAGTGCCA TTGCCAGTCA CGTCACAGCC GAGTGTCCCA GCAAGTGTCT GGTGGAGGTG 
CTGATCGCCA TCTGCAGCCT CACGTCCCCG CTGCTGTTCA CGGCCTCTGG GTACCTGTCA 
TTCAGCATCA TGAGAATCAT GGAGATCTTC AAAGATTACC CACCGGCCAT CAAGCAATCC 
TATGACGTGC TCCTGCTGCT TCTCATGCTG GAGCTGCTGC TGCAGGCCGG CCTCAACACG 
GGCACCGTCA TCCAGTGTGT GAGCTTCAAG CTGGGAGCAT CCTGGGATGC CGCACAGACC 
AGCCAGCAGG AGCGCCCCCC AGGGGAGGTG TCCAGGAGCC CACTGAAGGA GTTCGACAAG 
GAGAAGGCGT GGAGGGCCGT GGTGGTACAG ATGGCCCAGT GA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OCe38848
Clone ID Related Accession (Same CDS sequence) XM_014117268.2 , XM_014117268.1
Accession Version XM_014117268.2 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 1404bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2017-09-04
Organism Canis lupus familiaris(dog)
Product membrane protein MLC1 isoform X1
Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006592.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_014117268.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
ATGGACGGGC CAGCCCACTG TCTCCAGCTC ATTGACATTC GGGCAGTGGC ACCCCTTCCC 
TGGCTTCGGT GCTGCTGCGA CTTTTGTTCC CTTGTGAATA GCAATTGCTG GAAGCCCGAG 
GCCGGATGGC TCGGTAGCAG CCGCTTCTCG CCTGGAGACA GGAGACCAGG GCTCAGAAGC 
AAGCTGCTTA AGAGTGGCCC GGATAACAGC GGCAGAGGTG ACCCCGGCTG CGAGTGCCGC 
TGTCCTGGGG CTGCCAATGC GACGGTCTGT GCCCTGGGCA CCATGTCGCG GGACGAGCAG 
GGCAGGGAGG AGCTCGGTTA CGATCGCATG CCCACCCTGG AGCGCGGAAG GCCTGACGCA 
GGGAGCTACG CCCTGGACAC CAAGCCCAAT GACCTCCAGC TGGCGACGAG GCTGCCCCCA 
TGCTTCAGTC ACAAGACGTG GGTCCTCTCC GTGTTGATGG GGAGCTGTCT ACTTGTGACG 
TCGGGGTTTT CACTCTACCT GGGAAATGTG TTTCCCTCTG AAATGGATTA CTTACGTTGT 
GCTGCGGGAT CGTGCCTTCC CTCAGCAATC GTGAGCTTTG CCGTTTCACG GAGAAACATC 
AGTGCGATCC CCAACTTTCA GATCCTGTTT GTGTCCACAT TCGCAATCAC CACTACATGT 
TTAATCTGGT TTGGATGCAA ACTGGTCGTG AACCCCTCGG CAATAGACAT AAATTTTAAT 
CTGATTCTGC TCCTTCTGTT GGAGCTTTTC ATGGCGGCCA CAGTGATCAT CTCAGCACGA 
TCAAGTGAGG CGCACTGTAA GCAGAAGGGT CCCATGTTGG AGAGCGCCAA CATTTTGTAT 
GAAGTGGCAT TTCCTGCCCG GGTCCTAAAA TCCTATTCAG TCATCGAGGT GATTGCTGGT 
GTCTCTGCTG TCCTGGGTGG GGTTATCGCT CTGAACGTGG ATGACGCTGT CTCGGGGCCA 
CACCTCTCAG TGACGTTCTT TTGGATCTTA GTGGCCTGTT TTCCAAGTGC CATTGCCAGT 
CACGTCACAG CCGAGTGTCC CAGCAAGTGT CTGGTGGAGG TGCTGATCGC CATCTGCAGC 
CTCACGTCCC CGCTGCTGTT CACGGCCTCT GGGTACCTGT CATTCAGCAT CATGAGAATC 
ATGGAGATCT TCAAAGATTA CCCACCGGCC ATCAAGCAAT CCTATGACGT GCTCCTGCTG 
CTTCTCATGC TGGAGCTGCT GCTGCAGGCC GGCCTCAACA CGGGCACCGT CATCCAGTGT 
GTGAGCTTCA AGCTGGGAGC ATCCTGGGAT GCCGCACAGA CCAGCCAGCA GGAGCGCCCC 
CCAGGGGAGG TGTCCAGGAG CCCACTGAAG GAGTTCGACA AGGAGAAGGC GTGGAGGGCC 
GTGGTGGTAC AGATGGCCCA GTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_013972743.1
CDS140..1543
Translation

Target ORF information:

RefSeq Version XM_014117268.2
Organism Canis lupus familiaris(dog)
Definition Canis lupus familiaris megalencephalic leukoencephalopathy with subcortical cysts 1 (MLC1), transcript variant X1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 XM_014117268.2

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
ATGGACGGGC CAGCCCACTG TCTCCAGCTC ATTGACATTC GGGCAGTGGC ACCCCTTCCC 
TGGCTTCGGT GCTGCTGCGA CTTTTGTTCC CTTGTGAATA GCAATTGCTG GAAGCCCGAG 
GCCGGATGGC TCGGTAGCAG CCGCTTCTCG CCTGGAGACA GGAGACCAGG GCTCAGAAGC 
AAGCTGCTTA AGAGTGGCCC GGATAACAGC GGCAGAGGTG ACCCCGGCTG CGAGTGCCGC 
TGTCCTGGGG CTGCCAATGC GACGGTCTGT GCCCTGGGCA CCATGTCGCG GGACGAGCAG 
GGCAGGGAGG AGCTCGGTTA CGATCGCATG CCCACCCTGG AGCGCGGAAG GCCTGACGCA 
GGGAGCTACG CCCTGGACAC CAAGCCCAAT GACCTCCAGC TGGCGACGAG GCTGCCCCCA 
TGCTTCAGTC ACAAGACGTG GGTCCTCTCC GTGTTGATGG GGAGCTGTCT ACTTGTGACG 
TCGGGGTTTT CACTCTACCT GGGAAATGTG TTTCCCTCTG AAATGGATTA CTTACGTTGT 
GCTGCGGGAT CGTGCCTTCC CTCAGCAATC GTGAGCTTTG CCGTTTCACG GAGAAACATC 
AGTGCGATCC CCAACTTTCA GATCCTGTTT GTGTCCACAT TCGCAATCAC CACTACATGT 
TTAATCTGGT TTGGATGCAA ACTGGTCGTG AACCCCTCGG CAATAGACAT AAATTTTAAT 
CTGATTCTGC TCCTTCTGTT GGAGCTTTTC ATGGCGGCCA CAGTGATCAT CTCAGCACGA 
TCAAGTGAGG CGCACTGTAA GCAGAAGGGT CCCATGTTGG AGAGCGCCAA CATTTTGTAT 
GAAGTGGCAT TTCCTGCCCG GGTCCTAAAA TCCTATTCAG TCATCGAGGT GATTGCTGGT 
GTCTCTGCTG TCCTGGGTGG GGTTATCGCT CTGAACGTGG ATGACGCTGT CTCGGGGCCA 
CACCTCTCAG TGACGTTCTT TTGGATCTTA GTGGCCTGTT TTCCAAGTGC CATTGCCAGT 
CACGTCACAG CCGAGTGTCC CAGCAAGTGT CTGGTGGAGG TGCTGATCGC CATCTGCAGC 
CTCACGTCCC CGCTGCTGTT CACGGCCTCT GGGTACCTGT CATTCAGCAT CATGAGAATC 
ATGGAGATCT TCAAAGATTA CCCACCGGCC ATCAAGCAAT CCTATGACGT GCTCCTGCTG 
CTTCTCATGC TGGAGCTGCT GCTGCAGGCC GGCCTCAACA CGGGCACCGT CATCCAGTGT 
GTGAGCTTCA AGCTGGGAGC ATCCTGGGAT GCCGCACAGA CCAGCCAGCA GGAGCGCCCC 
CCAGGGGAGG TGTCCAGGAG CCCACTGAAG GAGTTCGACA AGGAGAAGGC GTGGAGGGCC 
GTGGTGGTAC AGATGGCCCA GTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OCe38848
Clone ID Related Accession (Same CDS sequence) XM_014117268.2 , XM_014117268.1
Accession Version XM_014117268.1 Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 1404bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2015-09-16
Organism Canis lupus familiaris(Dog)
Product membrane protein MLC1 isoform X1
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726078.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
ATGGACGGGC CAGCCCACTG TCTCCAGCTC ATTGACATTC GGGCAGTGGC ACCCCTTCCC 
TGGCTTCGGT GCTGCTGCGA CTTTTGTTCC CTTGTGAATA GCAATTGCTG GAAGCCCGAG 
GCCGGATGGC TCGGTAGCAG CCGCTTCTCG CCTGGAGACA GGAGACCAGG GCTCAGAAGC 
AAGCTGCTTA AGAGTGGCCC GGATAACAGC GGCAGAGGTG ACCCCGGCTG CGAGTGCCGC 
TGTCCTGGGG CTGCCAATGC GACGGTCTGT GCCCTGGGCA CCATGTCGCG GGACGAGCAG 
GGCAGGGAGG AGCTCGGTTA CGATCGCATG CCCACCCTGG AGCGCGGAAG GCCTGACGCA 
GGGAGCTACG CCCTGGACAC CAAGCCCAAT GACCTCCAGC TGGCGACGAG GCTGCCCCCA 
TGCTTCAGTC ACAAGACGTG GGTCCTCTCC GTGTTGATGG GGAGCTGTCT ACTTGTGACG 
TCGGGGTTTT CACTCTACCT GGGAAATGTG TTTCCCTCTG AAATGGATTA CTTACGTTGT 
GCTGCGGGAT CGTGCCTTCC CTCAGCAATC GTGAGCTTTG CCGTTTCACG GAGAAACATC 
AGTGCGATCC CCAACTTTCA GATCCTGTTT GTGTCCACAT TCGCAATCAC CACTACATGT 
TTAATCTGGT TTGGATGCAA ACTGGTCGTG AACCCCTCGG CAATAGACAT AAATTTTAAT 
CTGATTCTGC TCCTTCTGTT GGAGCTTTTC ATGGCGGCCA CAGTGATCAT CTCAGCACGA 
TCAAGTGAGG CGCACTGTAA GCAGAAGGGT CCCATGTTGG AGAGCGCCAA CATTTTGTAT 
GAAGTGGCAT TTCCTGCCCG GGTCCTAAAA TCCTATTCAG TCATCGAGGT GATTGCTGGT 
GTCTCTGCTG TCCTGGGTGG GGTTATCGCT CTGAACGTGG ATGACGCTGT CTCGGGGCCA 
CACCTCTCAG TGACGTTCTT TTGGATCTTA GTGGCCTGTT TTCCAAGTGC CATTGCCAGT 
CACGTCACAG CCGAGTGTCC CAGCAAGTGT CTGGTGGAGG TGCTGATCGC CATCTGCAGC 
CTCACGTCCC CGCTGCTGTT CACGGCCTCT GGGTACCTGT CATTCAGCAT CATGAGAATC 
ATGGAGATCT TCAAAGATTA CCCACCGGCC ATCAAGCAAT CCTATGACGT GCTCCTGCTG 
CTTCTCATGC TGGAGCTGCT GCTGCAGGCC GGCCTCAACA CGGGCACCGT CATCCAGTGT 
GTGAGCTTCA AGCTGGGAGC ATCCTGGGAT GCCGCACAGA CCAGCCAGCA GGAGCGCCCC 
CCAGGGGAGG TGTCCAGGAG CCCACTGAAG GAGTTCGACA AGGAGAAGGC GTGGAGGGCC 
GTGGTGGTAC AGATGGCCCA GTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_013972743.1
CDS16..1419
Translation

Target ORF information:

RefSeq Version XM_014117268.1
Organism Canis lupus familiaris(Dog)
Definition Canis lupus familiaris megalencephalic leukoencephalopathy with subcortical cysts 1 (MLC1), transcript variant X1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 XM_014117268.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
ATGGACGGGC CAGCCCACTG TCTCCAGCTC ATTGACATTC GGGCAGTGGC ACCCCTTCCC 
TGGCTTCGGT GCTGCTGCGA CTTTTGTTCC CTTGTGAATA GCAATTGCTG GAAGCCCGAG 
GCCGGATGGC TCGGTAGCAG CCGCTTCTCG CCTGGAGACA GGAGACCAGG GCTCAGAAGC 
AAGCTGCTTA AGAGTGGCCC GGATAACAGC GGCAGAGGTG ACCCCGGCTG CGAGTGCCGC 
TGTCCTGGGG CTGCCAATGC GACGGTCTGT GCCCTGGGCA CCATGTCGCG GGACGAGCAG 
GGCAGGGAGG AGCTCGGTTA CGATCGCATG CCCACCCTGG AGCGCGGAAG GCCTGACGCA 
GGGAGCTACG CCCTGGACAC CAAGCCCAAT GACCTCCAGC TGGCGACGAG GCTGCCCCCA 
TGCTTCAGTC ACAAGACGTG GGTCCTCTCC GTGTTGATGG GGAGCTGTCT ACTTGTGACG 
TCGGGGTTTT CACTCTACCT GGGAAATGTG TTTCCCTCTG AAATGGATTA CTTACGTTGT 
GCTGCGGGAT CGTGCCTTCC CTCAGCAATC GTGAGCTTTG CCGTTTCACG GAGAAACATC 
AGTGCGATCC CCAACTTTCA GATCCTGTTT GTGTCCACAT TCGCAATCAC CACTACATGT 
TTAATCTGGT TTGGATGCAA ACTGGTCGTG AACCCCTCGG CAATAGACAT AAATTTTAAT 
CTGATTCTGC TCCTTCTGTT GGAGCTTTTC ATGGCGGCCA CAGTGATCAT CTCAGCACGA 
TCAAGTGAGG CGCACTGTAA GCAGAAGGGT CCCATGTTGG AGAGCGCCAA CATTTTGTAT 
GAAGTGGCAT TTCCTGCCCG GGTCCTAAAA TCCTATTCAG TCATCGAGGT GATTGCTGGT 
GTCTCTGCTG TCCTGGGTGG GGTTATCGCT CTGAACGTGG ATGACGCTGT CTCGGGGCCA 
CACCTCTCAG TGACGTTCTT TTGGATCTTA GTGGCCTGTT TTCCAAGTGC CATTGCCAGT 
CACGTCACAG CCGAGTGTCC CAGCAAGTGT CTGGTGGAGG TGCTGATCGC CATCTGCAGC 
CTCACGTCCC CGCTGCTGTT CACGGCCTCT GGGTACCTGT CATTCAGCAT CATGAGAATC 
ATGGAGATCT TCAAAGATTA CCCACCGGCC ATCAAGCAAT CCTATGACGT GCTCCTGCTG 
CTTCTCATGC TGGAGCTGCT GCTGCAGGCC GGCCTCAACA CGGGCACCGT CATCCAGTGT 
GTGAGCTTCA AGCTGGGAGC ATCCTGGGAT GCCGCACAGA CCAGCCAGCA GGAGCGCCCC 
CCAGGGGAGG TGTCCAGGAG CCCACTGAAG GAGTTCGACA AGGAGAAGGC GTGGAGGGCC 
GTGGTGGTAC AGATGGCCCA GTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OCe38849
Clone ID Related Accession (Same CDS sequence) XM_014117269.1 , XM_014117269.2
Accession Version XM_014117269.1 Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 1401bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2015-09-16
Organism Canis lupus familiaris(Dog)
Product membrane protein MLC1 isoform X2
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726078.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
ATGGACGGGC CAGCCCACTG TCTCCAGCTC ATTGACATTC GGGCAGTGGC ACCCCTTCCC 
TGGCTTCGGT GCTGCTGCGA CTTTTGTTCC CTTGTGAATA GCAATTGCTG GAAGCCCGAG 
GCCGGATGGC TCGGTAGCAG CCGCTTCTCG CCTGGAGACA GGAGACCAGG GCTCAGAAGC 
AAGCTGCTTA AGAGTGGCCC GGATAACAGC GGCAGAGGTG ACCCCGGCTG CGAGTGCCGC 
TGTCCTGGGG CTGCCAATGC GACGGTCTGT GCCCTGGGCA CCATGTCGCG GGACGAGCAG 
GGCAGGGAGG AGCTCGGTTA CGATCGCATG CCCACCCTGG AGCGCGGAAG GCCTGACGCA 
GGGAGCTACG CCCTGGACAC CAAGCCCAAT GACCTCCAGC TGGCGACGAG GCTGCCCCCA 
TGCTTCAGTC ACAAGACGTG GGTCCTCTCC GTGTTGATGG GGAGCTGTCT ACTTGTGACG 
TCGGGGTTTT CACTCTACCT GGGAAATGTG TTTCCCTCTG AAATGGATTA CTTACGTTGT 
GCTGCGGGAT CGTGCCTTCC CTCAGCAATC GTGAGCTTTG CCGTTTCACG GAGAAACATC 
AGTGCGATCC CCAACTTTCA GATCCTGTTT GTGTCCACAT TCGCAATCAC CACTACATGT 
TTAATCTGGT TTGGATGCAA ACTGGTCGTG AACCCCTCGG CAATAGACAT AAATTTTAAT 
CTGATTCTGC TCCTTCTGTT GGAGCTTTTC ATGGCGGCCA CAGTGATCAT CTCAGCACGA 
TCAAGTGAGG CGCACTGTAA GCAGAAGGGT CCCATGTTGG AGAGCGCCAA CATTTTGTAT 
GAAGTGGCAT TTCCTGCCCG GGTCCTAAAA TCCTATTCAG TCATCGAGGT GATTGCTGGT 
GTCTCTGCTG TCCTGGGTGG GGTTATCGCT CTGAACGTGG ATGACGCTGT CTCGGGGCCA 
CACCTCTCAG TGACGTTCTT TTGGATCTTA GTGGCCTGTT TTCCAAGTGC CATTGCCAGT 
CACGTCACAG CCGAGTGTCC CAGCAAGTGT CTGGTGGAGG TGCTGATCGC CATCTGCAGC 
CTCACGTCCC CGCTGCTGTT CACGGCCTCT GGGTACCTGT CATTCAGCAT CATGAGAATC 
ATGGAGATCT TCAAAGATTA CCCACCGGCC ATCAAGCAAT CCTATGACGT GCTCCTGCTG 
CTTCTCATGC TGGAGCTGCT GCTGCAGGCC GGCCTCAACA CGGGCACCGT CATCCAGTGT 
GTGAGCTTCA AGCTGGGAGC ATCCTGGGAT GCCGCACAGA CCAGCCAGCA GGAGCGCCCC 
CCAGGGGAGG CTGAGAAAAG CCCGCGAGTT AGCAGAGATG TCCTGCCCGC CTGCTGTTGC 
TCCGGGGCCC AGGAGGAATA A

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_013972744.1
CDS16..1416
Translation

Target ORF information:

RefSeq Version XM_014117269.1
Organism Canis lupus familiaris(Dog)
Definition Canis lupus familiaris megalencephalic leukoencephalopathy with subcortical cysts 1 (MLC1), transcript variant X2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 XM_014117269.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
ATGGACGGGC CAGCCCACTG TCTCCAGCTC ATTGACATTC GGGCAGTGGC ACCCCTTCCC 
TGGCTTCGGT GCTGCTGCGA CTTTTGTTCC CTTGTGAATA GCAATTGCTG GAAGCCCGAG 
GCCGGATGGC TCGGTAGCAG CCGCTTCTCG CCTGGAGACA GGAGACCAGG GCTCAGAAGC 
AAGCTGCTTA AGAGTGGCCC GGATAACAGC GGCAGAGGTG ACCCCGGCTG CGAGTGCCGC 
TGTCCTGGGG CTGCCAATGC GACGGTCTGT GCCCTGGGCA CCATGTCGCG GGACGAGCAG 
GGCAGGGAGG AGCTCGGTTA CGATCGCATG CCCACCCTGG AGCGCGGAAG GCCTGACGCA 
GGGAGCTACG CCCTGGACAC CAAGCCCAAT GACCTCCAGC TGGCGACGAG GCTGCCCCCA 
TGCTTCAGTC ACAAGACGTG GGTCCTCTCC GTGTTGATGG GGAGCTGTCT ACTTGTGACG 
TCGGGGTTTT CACTCTACCT GGGAAATGTG TTTCCCTCTG AAATGGATTA CTTACGTTGT 
GCTGCGGGAT CGTGCCTTCC CTCAGCAATC GTGAGCTTTG CCGTTTCACG GAGAAACATC 
AGTGCGATCC CCAACTTTCA GATCCTGTTT GTGTCCACAT TCGCAATCAC CACTACATGT 
TTAATCTGGT TTGGATGCAA ACTGGTCGTG AACCCCTCGG CAATAGACAT AAATTTTAAT 
CTGATTCTGC TCCTTCTGTT GGAGCTTTTC ATGGCGGCCA CAGTGATCAT CTCAGCACGA 
TCAAGTGAGG CGCACTGTAA GCAGAAGGGT CCCATGTTGG AGAGCGCCAA CATTTTGTAT 
GAAGTGGCAT TTCCTGCCCG GGTCCTAAAA TCCTATTCAG TCATCGAGGT GATTGCTGGT 
GTCTCTGCTG TCCTGGGTGG GGTTATCGCT CTGAACGTGG ATGACGCTGT CTCGGGGCCA 
CACCTCTCAG TGACGTTCTT TTGGATCTTA GTGGCCTGTT TTCCAAGTGC CATTGCCAGT 
CACGTCACAG CCGAGTGTCC CAGCAAGTGT CTGGTGGAGG TGCTGATCGC CATCTGCAGC 
CTCACGTCCC CGCTGCTGTT CACGGCCTCT GGGTACCTGT CATTCAGCAT CATGAGAATC 
ATGGAGATCT TCAAAGATTA CCCACCGGCC ATCAAGCAAT CCTATGACGT GCTCCTGCTG 
CTTCTCATGC TGGAGCTGCT GCTGCAGGCC GGCCTCAACA CGGGCACCGT CATCCAGTGT 
GTGAGCTTCA AGCTGGGAGC ATCCTGGGAT GCCGCACAGA CCAGCCAGCA GGAGCGCCCC 
CCAGGGGAGG CTGAGAAAAG CCCGCGAGTT AGCAGAGATG TCCTGCCCGC CTGCTGTTGC 
TCCGGGGCCC AGGAGGAATA A

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OCe38849
Clone ID Related Accession (Same CDS sequence) XM_014117269.1 , XM_014117269.2
Accession Version XM_014117269.2 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 1401bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2017-09-04
Organism Canis lupus familiaris(dog)
Product membrane protein MLC1 isoform X2
Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006592.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_014117269.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
ATGGACGGGC CAGCCCACTG TCTCCAGCTC ATTGACATTC GGGCAGTGGC ACCCCTTCCC 
TGGCTTCGGT GCTGCTGCGA CTTTTGTTCC CTTGTGAATA GCAATTGCTG GAAGCCCGAG 
GCCGGATGGC TCGGTAGCAG CCGCTTCTCG CCTGGAGACA GGAGACCAGG GCTCAGAAGC 
AAGCTGCTTA AGAGTGGCCC GGATAACAGC GGCAGAGGTG ACCCCGGCTG CGAGTGCCGC 
TGTCCTGGGG CTGCCAATGC GACGGTCTGT GCCCTGGGCA CCATGTCGCG GGACGAGCAG 
GGCAGGGAGG AGCTCGGTTA CGATCGCATG CCCACCCTGG AGCGCGGAAG GCCTGACGCA 
GGGAGCTACG CCCTGGACAC CAAGCCCAAT GACCTCCAGC TGGCGACGAG GCTGCCCCCA 
TGCTTCAGTC ACAAGACGTG GGTCCTCTCC GTGTTGATGG GGAGCTGTCT ACTTGTGACG 
TCGGGGTTTT CACTCTACCT GGGAAATGTG TTTCCCTCTG AAATGGATTA CTTACGTTGT 
GCTGCGGGAT CGTGCCTTCC CTCAGCAATC GTGAGCTTTG CCGTTTCACG GAGAAACATC 
AGTGCGATCC CCAACTTTCA GATCCTGTTT GTGTCCACAT TCGCAATCAC CACTACATGT 
TTAATCTGGT TTGGATGCAA ACTGGTCGTG AACCCCTCGG CAATAGACAT AAATTTTAAT 
CTGATTCTGC TCCTTCTGTT GGAGCTTTTC ATGGCGGCCA CAGTGATCAT CTCAGCACGA 
TCAAGTGAGG CGCACTGTAA GCAGAAGGGT CCCATGTTGG AGAGCGCCAA CATTTTGTAT 
GAAGTGGCAT TTCCTGCCCG GGTCCTAAAA TCCTATTCAG TCATCGAGGT GATTGCTGGT 
GTCTCTGCTG TCCTGGGTGG GGTTATCGCT CTGAACGTGG ATGACGCTGT CTCGGGGCCA 
CACCTCTCAG TGACGTTCTT TTGGATCTTA GTGGCCTGTT TTCCAAGTGC CATTGCCAGT 
CACGTCACAG CCGAGTGTCC CAGCAAGTGT CTGGTGGAGG TGCTGATCGC CATCTGCAGC 
CTCACGTCCC CGCTGCTGTT CACGGCCTCT GGGTACCTGT CATTCAGCAT CATGAGAATC 
ATGGAGATCT TCAAAGATTA CCCACCGGCC ATCAAGCAAT CCTATGACGT GCTCCTGCTG 
CTTCTCATGC TGGAGCTGCT GCTGCAGGCC GGCCTCAACA CGGGCACCGT CATCCAGTGT 
GTGAGCTTCA AGCTGGGAGC ATCCTGGGAT GCCGCACAGA CCAGCCAGCA GGAGCGCCCC 
CCAGGGGAGG CTGAGAAAAG CCCGCGAGTT AGCAGAGATG TCCTGCCCGC CTGCTGTTGC 
TCCGGGGCCC AGGAGGAATA A

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_013972744.1
CDS140..1540
Translation

Target ORF information:

RefSeq Version XM_014117269.2
Organism Canis lupus familiaris(dog)
Definition Canis lupus familiaris megalencephalic leukoencephalopathy with subcortical cysts 1 (MLC1), transcript variant X2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 XM_014117269.2

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
ATGGACGGGC CAGCCCACTG TCTCCAGCTC ATTGACATTC GGGCAGTGGC ACCCCTTCCC 
TGGCTTCGGT GCTGCTGCGA CTTTTGTTCC CTTGTGAATA GCAATTGCTG GAAGCCCGAG 
GCCGGATGGC TCGGTAGCAG CCGCTTCTCG CCTGGAGACA GGAGACCAGG GCTCAGAAGC 
AAGCTGCTTA AGAGTGGCCC GGATAACAGC GGCAGAGGTG ACCCCGGCTG CGAGTGCCGC 
TGTCCTGGGG CTGCCAATGC GACGGTCTGT GCCCTGGGCA CCATGTCGCG GGACGAGCAG 
GGCAGGGAGG AGCTCGGTTA CGATCGCATG CCCACCCTGG AGCGCGGAAG GCCTGACGCA 
GGGAGCTACG CCCTGGACAC CAAGCCCAAT GACCTCCAGC TGGCGACGAG GCTGCCCCCA 
TGCTTCAGTC ACAAGACGTG GGTCCTCTCC GTGTTGATGG GGAGCTGTCT ACTTGTGACG 
TCGGGGTTTT CACTCTACCT GGGAAATGTG TTTCCCTCTG AAATGGATTA CTTACGTTGT 
GCTGCGGGAT CGTGCCTTCC CTCAGCAATC GTGAGCTTTG CCGTTTCACG GAGAAACATC 
AGTGCGATCC CCAACTTTCA GATCCTGTTT GTGTCCACAT TCGCAATCAC CACTACATGT 
TTAATCTGGT TTGGATGCAA ACTGGTCGTG AACCCCTCGG CAATAGACAT AAATTTTAAT 
CTGATTCTGC TCCTTCTGTT GGAGCTTTTC ATGGCGGCCA CAGTGATCAT CTCAGCACGA 
TCAAGTGAGG CGCACTGTAA GCAGAAGGGT CCCATGTTGG AGAGCGCCAA CATTTTGTAT 
GAAGTGGCAT TTCCTGCCCG GGTCCTAAAA TCCTATTCAG TCATCGAGGT GATTGCTGGT 
GTCTCTGCTG TCCTGGGTGG GGTTATCGCT CTGAACGTGG ATGACGCTGT CTCGGGGCCA 
CACCTCTCAG TGACGTTCTT TTGGATCTTA GTGGCCTGTT TTCCAAGTGC CATTGCCAGT 
CACGTCACAG CCGAGTGTCC CAGCAAGTGT CTGGTGGAGG TGCTGATCGC CATCTGCAGC 
CTCACGTCCC CGCTGCTGTT CACGGCCTCT GGGTACCTGT CATTCAGCAT CATGAGAATC 
ATGGAGATCT TCAAAGATTA CCCACCGGCC ATCAAGCAAT CCTATGACGT GCTCCTGCTG 
CTTCTCATGC TGGAGCTGCT GCTGCAGGCC GGCCTCAACA CGGGCACCGT CATCCAGTGT 
GTGAGCTTCA AGCTGGGAGC ATCCTGGGAT GCCGCACAGA CCAGCCAGCA GGAGCGCCCC 
CCAGGGGAGG CTGAGAAAAG CCCGCGAGTT AGCAGAGATG TCCTGCCCGC CTGCTGTTGC 
TCCGGGGCCC AGGAGGAATA A

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

PubMed