NHSA cDNA ORF clone, Danio rerio(Zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	NHSA
Entrez Gene ID	100150332
Full Name	Nance-Horan syndrome a (congenital cataracts and dental anomalies)
Synonyms	nhs
General protein information	Preferred Names Nance-Horan syndrome a (congenital cataracts and dental anomalies) Names Nance-Horan syndrome protein
Gene Type	protein-coding
Organism	Danio rerio(Zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
XM_021470045.1	XP_021325720.1	Nance-Horan syndrome protein isoform X3
XM_001919305.6	XP_001919340.4	Nance-Horan syndrome protein isoform X1
XM_021470044.1	XP_021325719.1	Nance-Horan syndrome protein isoform X1
XM_001919305.7	XP_001919340.4	Nance-Horan syndrome protein isoform X2
XM_009297102.2	XP_009295377.1	Nance-Horan syndrome protein isoform X2
XM_021470046.1	XP_021325721.1	Nance-Horan syndrome protein isoform X4
XM_009297103.2	XP_009295378.1	Nance-Horan syndrome protein isoform X3

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	NHS	XP_548877.2
Bos taurus (cattle)	NHS	NP_001192657.1
Mus musculus (house mouse)	Nhs	NP_001074521.1
	NHS	XP_001104229.2
Rattus norvegicus (Norway rat)	Nhs	NP_001178662.1
Danio rerio (zebrafish)	nhs	XP_005169435.1
Gallus gallus (chicken)	NHS	XP_416815.4
Danio rerio (zebrafish)	nhsa	XP_001919340.4
Xenopus tropicalis (tropical clawed frog)	LOC100498628	XP_002934404.2
Homo sapiens (human)	NHS	NP_938011.1
Pan troglodytes (chimpanzee)	NHS	XP_520958.3

Gene Ontology

The following NHSA gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the NHSA cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODa151613	XM_021470046.1 Latest version!	Danio rerio Nance-Horan syndrome a (congenital cataracts and dental anomalies) (nhsa), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$797.30 $1139.00
ODa151611	XM_021470044.1 Latest version!	Danio rerio Nance-Horan syndrome a (congenital cataracts and dental anomalies) (nhsa), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$881.30 $1259.00
ODa151612	XM_021470045.1 Latest version!	Danio rerio Nance-Horan syndrome a (congenital cataracts and dental anomalies) (nhsa), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$881.30 $1259.00
ODa28082	XM_001919305.7 Latest version!	Danio rerio Nance-Horan syndrome a (congenital cataracts and dental anomalies) (nhsa), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00
ODa48870	XM_009297102.2 Latest version!	Danio rerio Nance-Horan syndrome a (congenital cataracts and dental anomalies) (nhsa), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00
ODa48871	XM_009297103.2 Latest version!	Danio rerio Nance-Horan syndrome a (congenital cataracts and dental anomalies) (nhsa), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$797.30 $1139.00
View Old Accession Versions >>
ODa28082	XM_001919305.6	Danio rerio Nance-Horan syndrome a (congenital cataracts and dental anomalies) (nhsa), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

NHSA ( XM_021470046.1 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_021325721.1 Danio rerio Nance-Horan syndrome a (congenital cataracts and dental anomalies) (nhsa), transcript variant X4, mRNA.

Price & Availability↑

CloneID	ODa151613
Clone ID Related Accession (Same CDS sequence)	XM_021470046.1
Accession Version	XM_021470046.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4404bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	Nance-Horan syndrome protein isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007134.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTCTCG GATGCTGCAT ACCACTGAGG TCTAAAGCCT TGTCGAATCT GGACATCGAG 
AGTAAATTGA GTGGACATTA CCAGGCTCCA TGGCACCTGC AGAGGAACGT GTTTCTGCCG 
TCCACGAGGC CCGCGTGTGT GGAGGAGCTG CACCGACACG CCAAACAGAG CCTGAGGGCC 
ATGTACAGGG ATCAGCAGCA GAGGCAGCAG GAGAGCGAGA GAGAGCGGCG GGTCACCATC 
TCCATCTCCA TGCTCCCGTC GGTGCCCTCA TACCCATCGC CCCGCGCCCA CAGGAGACGC 
CAGCAGAGGG ACACACATCT TAGCACGGAG AGGACGGAGA GAGACACAGA GACTCAGACA 
ACACAGACAC AGTTTCAAAG CACCCGGTCC TCCTCCCCTA CTGAGTGTTG TCATTTTTCC 
CCATGGAGCA GAAAGGTTAC TCCTCCAGGT GATTCAGACT CAGAGCTGGT GGCTTTAGGC 
CACTGGCCGA AGTGCCCGAT CCCAAACGTG CCCTCAACGC TGGACAAACA GACCAACTGG 
GCAGGACTCT TACCATTACC GACCCCAGAA GAGAAAATGA AGACCGACTC TCAAGTCATC 
AACTCATGCA TCGTCCCTAT TAACGTCACA GAAGAGGGCG CCAGCGCTTC TTCCCCCTCA 
GCGATATCAG CTTTCCTAAG GGTTGGGTTC GACAGAGAGG CCAGTGTACG CTGCTCGCTA 
GTGCATTCGC AGTCCGTGCT TCAGCGGCGG AGGAAGCTCA GGAGACGGAA AACCATCACT 
GGCATTCCCA GACGAGTGCA GCAGGATTTG GATTCTGACG AGTCTCCAGT GGCAAGAGAA 
CGTACTGTGA TAGTACACAC AAATCCTGAA ATTGGCCAGG AGGATCTAAC GCTCTCTCAT 
CTCAAAACCA AGGACTCTGG TTGCCAAACT GAAGAAATCC TAATTGCTGG GGCTGCACCC 
TCACGTAGAC GGATTAGAGC ACAAAGAGGG CAGTGCATGA CCGTGTCTCT GTCCCATTCG 
ACAGGCAACA TATCTTCTCT GCCTGACAAC TCTGACGCTA TGTTCACTGC CTCGGCGGGC 
TCCCGTCTGC GTTCCCGAAG CCTTCCGCGA GAGGGAGGCC GACTGGTGGA TGAAGGTCAT 
GATGACAGCG ATGAAGAGGA GGATGAGGAA GAATTGTCTC CGTTTGAGGC AGAGGACTTC 
CTACCAGTTC CAGGGGAGCA TATATTAAAA GATGACGAGG AAAGCACTGA TGATCAGACG 
ATCCCAGAAG GACAGCATAA TAGTTTGAAA TTCAAGCAGC ATGCTGGAAG TACAGACCAT 
GAATGGTTGG AGAGGGGTCG ATCCCGTCTG CCTCGGAAAG CTGACATGGG TAGTTGTGAG 
ATTTCCTCTA GTTCTGACAC ATTCAGTAGC CCTATTCACT CCATTTCCAA TGCTGGTGTG 
CTGACTAGCC AGATGGACCA CAAGGAGGAT CACCAGTCCT CAAGCGGCAA CTGGAGTGGT 
AGTAGTTCAA CATGTCCCTC CCAGACGTCG GAAACCATTC CACCTGCAGC CTCACCCCCA 
CTCACCGGAT CATCCCACTG TGATTCAGAG CTATCGCTGA ATGCAGCTAC CAATACTAAT 
GATGATTCCA CTGGCTTCAT TATTGATCCG TATCCTGGGG ACCGATTGCA AGGGCTCCGC 
AGCCATAGAA CGGGTTCATT CACTTCCACT GCAACTGACA TGCTTGACGA TGCTGGAGTC 
AGCACTGCTA GTGAGGGTGA ATGGAGTTAT CCCCAATACA GGCATAACAT ACCTTGCCAC 
CCTGACTTCA GTCCTGAGCA CTCAAGGGCA GAGAACATAC TCGAATGTCC CAGTTTTACT 
AGCATAGCCA CTTTTGAAAG TTTAACAGAT AGGCCACCTT CTGAAAAGGC AGACACCACC 
TCACACTATT CTGTAGATGC CGAGGGCTAC TATACCTCCA TGCACTTTGA CTGTGGTCTT 
AAAGGTAGCA AAAGCTTCAC ATATAACTAT GCATCTGTAG ACCAGCAGCA GGCAGACTAT 
GGACATCAAA CAAACACACT TGGGAGACAT AGTCTCTCTT TAAGGAAACC AAAGGTGAAG 
CCATCCCCAC CAAAACGCAG CTCATCATTG AGGAAATTAA GCAGCCATGT TGGACCTCCC 
ACAGACAAGA ATGAACCAAA GATTGCAGGT GATCAGAACA AATCAATGTC TTCCGGGGAG 
AGAACACATA ACATGGGATT AAACGGAACC TCCTTGAGTC AGCTGGAGAG CGAGGAGCCT 
ATGGGAGCAT GGGGTGTTGA GAGTTCAGCC GAGATTCCAG ATGTTGCTTT GTTTGGCTCC 
ACAGAAACGC ATTCCTTCAA GGATGAGGGA GCTGTCCAGT CAGACTATGC AGATCTCTGG 
CTTCTTAATG ACTTAAAATC CAACGATCCC TACAGATCAT TGTCAAATTC CAGCACAGCT 
ACGGGTACCA CTGTCATTGA ATGCATTAAG TCACAGGAGA GCTCAGAGTC CCAGACCTCA 
CAGTCAGGTT CGAGAGCCAC AACTCCATCA CTACCATCAG TGGAGTGCGA GTTTAGGCTA 
CCCTCACCAG AAAAGCTGGT AGGTCTAGCA TCACCTTCAA GTGGCTACTC AAGCCAATCA 
GAGACACCAA CATCTTCTCT TCCATCTGCT TTCTTTCCAA ACGCTCACCC AATGTCCCCT 
ACTGGTGGCA AGAGGAAACC CAAAGTCCCA GAGAGGAAGT CCTCACTTGC TTCTTTGCAG 
CAGAACTCAT CCAAAAGGGA ACTTGAATTG CCTATTATAC CTCCCACCCA CCTTGACCTA 
AGTGCCCTTC CCAATGTCAG CAAGCCTTCG GTTCACAAAA ACCAGATGCA CGTCCTTCAC 
CACAGCAAGA AAAAAGCAGC AGAAACCAAG CGTGAGTGTC AAGCATCTTC AGTTTCAAGT 
GATGATCTAC CAGTTAGCGG TGCTTTAGCC ATCACTCCTA CAATACTTCG TTCGGTTCAG 
CTACGAGCAG TTGGAAAATC TGGAGAAGGG AGTCATGATC AGCCTATAAC TGATACTGCC 
ACTAGGCCTA AGTGTCCCAC TGTGACTATC ATCACACCAC CTACTCACAA CAAATCCCAC 
GAAAGTAGAA AACCTCCACC CTATAGACCT CTTCTCACAG AACCCACCTC TCAGGACAAT 
TGTGAATCAC TGTTTACCCC AGTAAATGGA CTAGCTGCTA AGAATACTCC AAGCCATTTT 
GGTGCAAGAA ATCGATATGA CCGATTAGCC CCATCACCTC TTTGGAGCAT GACTGCTTTT 
AAGGCTCAGA CTGAGCCGGT AAACCTGGAT GAAGTGATCT CTGAGAATTC TCAAGAAGCT 
GCAGAAGAGA AGATCCCCTC AGATTATTTA TTTGCCCAGA GTAGCATGGA AGTACAGCAA 
AACCAGTCAT TGAGATCTGA GCCAAACGAC AGAAGCCCTG GTCAGAATGG TGTAGCAAAA 
AGGTCAGATA GTCTTGACCA AGTGCCTGAT ATTGTCAATC AAAGATCAGA GACATTGCAA 
GCTTACAAAA TCAGTGGTGC TGGAGAAGAA TCTGTAACAT CAGGTGTTCC AACCAGAAGT 
GCCTCACAAG AATATGTAGA GGAAGGGTCT ACACCAGACA CAGAGGACTA TTTTAGCAGA 
GAGTCCACAC CAAGTGATCC ATCTGTGTCC CCTCTGATAG ACGAATCCAA AACTGAAGAT 
GATAGCGTTT TCCTATCTCC AAGTAAAAGT CGCACGACTG AAGATCTCTT TGCCATGATT 
CACAGGTCGA AACGAAAAAT GCTGGGCAGG AAAGATTCCT CTGAAACATT AGTGCGAGGC 
CGTTTAGCCG TTGTTTCTGG GAATGGTCCT GCTACAAATC CAACAACCCC GCCAAGCACC 
CCAAGCATAC CTGCAAGTCC ATCTGCTCAA ACGGGGGCTC AGAAAACACC GGGTCCAATC 
TACAGGAGTG TCAAAAAGTC CAGTACCTCG AATGAAGAGT TCAAACTGCT GCTTTTAAAA 
AAGGGAAGTC GCTCTGACTC TAGTTATCGA ATGTCAGCTA CAGAGATCCT AAAGAGCCCC 
ATCGCGCCCA AAAGCCCAGG TGAGCTCTTG CTTGAGGGTT CTAGACCACT CGAGGAACCA 
CTTTCCCCTA TACAACAGCA ATTTCCAGAG GGACCTCCAG AGCAAATACC TAGCCCTTTT 
CCAAAAACTT TTGCTGAGGG CTTCTCCATG AAAGCCTTTC CCACATCTGC CTCAGCTAGA 
CAAGGCCGGT CCAGGATGCC TCCAGCTGCG AACAGCAGCC GCTACAGTAC ACGCAGTCGG 
CTCTACTGCG CTCCCATGCA GGCCATATCA GAAGGTGAAA CCGAAAACTC AGATGGAAGC 
CCACATGATG ACCGTTCCTC CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021325721.1
CDS	230..4633
Translation	MALGCCIPLRSKALSNLDIESKLSGHYQAPWHLQRNVFLPSTRPACVEELHRHAKQSLRAMYRDQQQRQQESERERRVTISISMLPSVPSYPSPRAHRRRQQRDTHLSTERTERDTETQTTQTQFQSTRSSSPTECCHFSPWSRKVTPPGDSDSELVALGHWPKCPIPNVPSTLDKQTNWAGLLPLPTPEEKMKTDSQVINSCIVPINVTEEGASASSPSAISAFLRVGFDREASVRCSLVHSQSVLQRRRKLRRRKTITGIPRRVQQDLDSDESPVARERTVIVHTNPEIGQEDLTLSHLKTKDSGCQTEEILIAGAAPSRRRIRAQRGQCMTVSLSHSTGNISSLPDNSDAMFTASAGSRLRSRSLPREGGRLVDEGHDDSDEEEDEEELSPFEAEDFLPVPGEHILKDDEESTDDQTIPEGQHNSLKFKQHAGSTDHEWLERGRSRLPRKADMGSCEISSSSDTFSSPIHSISNAGVLTSQMDHKEDHQSSSGNWSGSSSTCPSQTSETIPPAASPPLTGSSHCDSELSLNAATNTNDDSTGFIIDPYPGDRLQGLRSHRTGSFTSTATDMLDDAGVSTASEGEWSYPQYRHNIPCHPDFSPEHSRAENILECPSFTSIATFESLTDRPPSEKADTTSHYSVDAEGYYTSMHFDCGLKGSKSFTYNYASVDQQQADYGHQTNTLGRHSLSLRKPKVKPSPPKRSSSLRKLSSHVGPPTDKNEPKIAGDQNKSMSSGERTHNMGLNGTSLSQLESEEPMGAWGVESSAEIPDVALFGSTETHSFKDEGAVQSDYADLWLLNDLKSNDPYRSLSNSSTATGTTVIECIKSQESSESQTSQSGSRATTPSLPSVECEFRLPSPEKLVGLASPSSGYSSQSETPTSSLPSAFFPNAHPMSPTGGKRKPKVPERKSSLASLQQNSSKRELELPIIPPTHLDLSALPNVSKPSVHKNQMHVLHHSKKKAAETKRECQASSVSSDDLPVSGALAITPTILRSVQLRAVGKSGEGSHDQPITDTATRPKCPTVTIITPPTHNKSHESRKPPPYRPLLTEPTSQDNCESLFTPVNGLAAKNTPSHFGARNRYDRLAPSPLWSMTAFKAQTEPVNLDEVISENSQEAAEEKIPSDYLFAQSSMEVQQNQSLRSEPNDRSPGQNGVAKRSDSLDQVPDIVNQRSETLQAYKISGAGEESVTSGVPTRSASQEYVEEGSTPDTEDYFSRESTPSDPSVSPLIDESKTEDDSVFLSPSKSRTTEDLFAMIHRSKRKMLGRKDSSETLVRGRLAVVSGNGPATNPTTPPSTPSIPASPSAQTGAQKTPGPIYRSVKKSSTSNEEFKLLLLKKGSRSDSSYRMSATEILKSPIAPKSPGELLLEGSRPLEEPLSPIQQQFPEGPPEQIPSPFPKTFAEGFSMKAFPTSASARQGRSRMPPAANSSRYSTRSRLYCAPMQAISEGETENSDGSPHDDRSS

Target ORF information:

RefSeq Version	XM_021470046.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio Nance-Horan syndrome a (congenital cataracts and dental anomalies) (nhsa), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021470046.1

ORF Insert Sequence:

ATGGCTCTCG GATGCTGCAT ACCACTGAGG TCTAAAGCCT TGTCGAATCT GGACATCGAG 
AGTAAATTGA GTGGACATTA CCAGGCTCCA TGGCACCTGC AGAGGAACGT GTTTCTGCCG 
TCCACGAGGC CCGCGTGTGT GGAGGAGCTG CACCGACACG CCAAACAGAG CCTGAGGGCC 
ATGTACAGGG ATCAGCAGCA GAGGCAGCAG GAGAGCGAGA GAGAGCGGCG GGTCACCATC 
TCCATCTCCA TGCTCCCGTC GGTGCCCTCA TACCCATCGC CCCGCGCCCA CAGGAGACGC 
CAGCAGAGGG ACACACATCT TAGCACGGAG AGGACGGAGA GAGACACAGA GACTCAGACA 
ACACAGACAC AGTTTCAAAG CACCCGGTCC TCCTCCCCTA CTGAGTGTTG TCATTTTTCC 
CCATGGAGCA GAAAGGTTAC TCCTCCAGGT GATTCAGACT CAGAGCTGGT GGCTTTAGGC 
CACTGGCCGA AGTGCCCGAT CCCAAACGTG CCCTCAACGC TGGACAAACA GACCAACTGG 
GCAGGACTCT TACCATTACC GACCCCAGAA GAGAAAATGA AGACCGACTC TCAAGTCATC 
AACTCATGCA TCGTCCCTAT TAACGTCACA GAAGAGGGCG CCAGCGCTTC TTCCCCCTCA 
GCGATATCAG CTTTCCTAAG GGTTGGGTTC GACAGAGAGG CCAGTGTACG CTGCTCGCTA 
GTGCATTCGC AGTCCGTGCT TCAGCGGCGG AGGAAGCTCA GGAGACGGAA AACCATCACT 
GGCATTCCCA GACGAGTGCA GCAGGATTTG GATTCTGACG AGTCTCCAGT GGCAAGAGAA 
CGTACTGTGA TAGTACACAC AAATCCTGAA ATTGGCCAGG AGGATCTAAC GCTCTCTCAT 
CTCAAAACCA AGGACTCTGG TTGCCAAACT GAAGAAATCC TAATTGCTGG GGCTGCACCC 
TCACGTAGAC GGATTAGAGC ACAAAGAGGG CAGTGCATGA CCGTGTCTCT GTCCCATTCG 
ACAGGCAACA TATCTTCTCT GCCTGACAAC TCTGACGCTA TGTTCACTGC CTCGGCGGGC 
TCCCGTCTGC GTTCCCGAAG CCTTCCGCGA GAGGGAGGCC GACTGGTGGA TGAAGGTCAT 
GATGACAGCG ATGAAGAGGA GGATGAGGAA GAATTGTCTC CGTTTGAGGC AGAGGACTTC 
CTACCAGTTC CAGGGGAGCA TATATTAAAA GATGACGAGG AAAGCACTGA TGATCAGACG 
ATCCCAGAAG GACAGCATAA TAGTTTGAAA TTCAAGCAGC ATGCTGGAAG TACAGACCAT 
GAATGGTTGG AGAGGGGTCG ATCCCGTCTG CCTCGGAAAG CTGACATGGG TAGTTGTGAG 
ATTTCCTCTA GTTCTGACAC ATTCAGTAGC CCTATTCACT CCATTTCCAA TGCTGGTGTG 
CTGACTAGCC AGATGGACCA CAAGGAGGAT CACCAGTCCT CAAGCGGCAA CTGGAGTGGT 
AGTAGTTCAA CATGTCCCTC CCAGACGTCG GAAACCATTC CACCTGCAGC CTCACCCCCA 
CTCACCGGAT CATCCCACTG TGATTCAGAG CTATCGCTGA ATGCAGCTAC CAATACTAAT 
GATGATTCCA CTGGCTTCAT TATTGATCCG TATCCTGGGG ACCGATTGCA AGGGCTCCGC 
AGCCATAGAA CGGGTTCATT CACTTCCACT GCAACTGACA TGCTTGACGA TGCTGGAGTC 
AGCACTGCTA GTGAGGGTGA ATGGAGTTAT CCCCAATACA GGCATAACAT ACCTTGCCAC 
CCTGACTTCA GTCCTGAGCA CTCAAGGGCA GAGAACATAC TCGAATGTCC CAGTTTTACT 
AGCATAGCCA CTTTTGAAAG TTTAACAGAT AGGCCACCTT CTGAAAAGGC AGACACCACC 
TCACACTATT CTGTAGATGC CGAGGGCTAC TATACCTCCA TGCACTTTGA CTGTGGTCTT 
AAAGGTAGCA AAAGCTTCAC ATATAACTAT GCATCTGTAG ACCAGCAGCA GGCAGACTAT 
GGACATCAAA CAAACACACT TGGGAGACAT AGTCTCTCTT TAAGGAAACC AAAGGTGAAG 
CCATCCCCAC CAAAACGCAG CTCATCATTG AGGAAATTAA GCAGCCATGT TGGACCTCCC 
ACAGACAAGA ATGAACCAAA GATTGCAGGT GATCAGAACA AATCAATGTC TTCCGGGGAG 
AGAACACATA ACATGGGATT AAACGGAACC TCCTTGAGTC AGCTGGAGAG CGAGGAGCCT 
ATGGGAGCAT GGGGTGTTGA GAGTTCAGCC GAGATTCCAG ATGTTGCTTT GTTTGGCTCC 
ACAGAAACGC ATTCCTTCAA GGATGAGGGA GCTGTCCAGT CAGACTATGC AGATCTCTGG 
CTTCTTAATG ACTTAAAATC CAACGATCCC TACAGATCAT TGTCAAATTC CAGCACAGCT 
ACGGGTACCA CTGTCATTGA ATGCATTAAG TCACAGGAGA GCTCAGAGTC CCAGACCTCA 
CAGTCAGGTT CGAGAGCCAC AACTCCATCA CTACCATCAG TGGAGTGCGA GTTTAGGCTA 
CCCTCACCAG AAAAGCTGGT AGGTCTAGCA TCACCTTCAA GTGGCTACTC AAGCCAATCA 
GAGACACCAA CATCTTCTCT TCCATCTGCT TTCTTTCCAA ACGCTCACCC AATGTCCCCT 
ACTGGTGGCA AGAGGAAACC CAAAGTCCCA GAGAGGAAGT CCTCACTTGC TTCTTTGCAG 
CAGAACTCAT CCAAAAGGGA ACTTGAATTG CCTATTATAC CTCCCACCCA CCTTGACCTA 
AGTGCCCTTC CCAATGTCAG CAAGCCTTCG GTTCACAAAA ACCAGATGCA CGTCCTTCAC 
CACAGCAAGA AAAAAGCAGC AGAAACCAAG CGTGAGTGTC AAGCATCTTC AGTTTCAAGT 
GATGATCTAC CAGTTAGCGG TGCTTTAGCC ATCACTCCTA CAATACTTCG TTCGGTTCAG 
CTACGAGCAG TTGGAAAATC TGGAGAAGGG AGTCATGATC AGCCTATAAC TGATACTGCC 
ACTAGGCCTA AGTGTCCCAC TGTGACTATC ATCACACCAC CTACTCACAA CAAATCCCAC 
GAAAGTAGAA AACCTCCACC CTATAGACCT CTTCTCACAG AACCCACCTC TCAGGACAAT 
TGTGAATCAC TGTTTACCCC AGTAAATGGA CTAGCTGCTA AGAATACTCC AAGCCATTTT 
GGTGCAAGAA ATCGATATGA CCGATTAGCC CCATCACCTC TTTGGAGCAT GACTGCTTTT 
AAGGCTCAGA CTGAGCCGGT AAACCTGGAT GAAGTGATCT CTGAGAATTC TCAAGAAGCT 
GCAGAAGAGA AGATCCCCTC AGATTATTTA TTTGCCCAGA GTAGCATGGA AGTACAGCAA 
AACCAGTCAT TGAGATCTGA GCCAAACGAC AGAAGCCCTG GTCAGAATGG TGTAGCAAAA 
AGGTCAGATA GTCTTGACCA AGTGCCTGAT ATTGTCAATC AAAGATCAGA GACATTGCAA 
GCTTACAAAA TCAGTGGTGC TGGAGAAGAA TCTGTAACAT CAGGTGTTCC AACCAGAAGT 
GCCTCACAAG AATATGTAGA GGAAGGGTCT ACACCAGACA CAGAGGACTA TTTTAGCAGA 
GAGTCCACAC CAAGTGATCC ATCTGTGTCC CCTCTGATAG ACGAATCCAA AACTGAAGAT 
GATAGCGTTT TCCTATCTCC AAGTAAAAGT CGCACGACTG AAGATCTCTT TGCCATGATT 
CACAGGTCGA AACGAAAAAT GCTGGGCAGG AAAGATTCCT CTGAAACATT AGTGCGAGGC 
CGTTTAGCCG TTGTTTCTGG GAATGGTCCT GCTACAAATC CAACAACCCC GCCAAGCACC 
CCAAGCATAC CTGCAAGTCC ATCTGCTCAA ACGGGGGCTC AGAAAACACC GGGTCCAATC 
TACAGGAGTG TCAAAAAGTC CAGTACCTCG AATGAAGAGT TCAAACTGCT GCTTTTAAAA 
AAGGGAAGTC GCTCTGACTC TAGTTATCGA ATGTCAGCTA CAGAGATCCT AAAGAGCCCC 
ATCGCGCCCA AAAGCCCAGG TGAGCTCTTG CTTGAGGGTT CTAGACCACT CGAGGAACCA 
CTTTCCCCTA TACAACAGCA ATTTCCAGAG GGACCTCCAG AGCAAATACC TAGCCCTTTT 
CCAAAAACTT TTGCTGAGGG CTTCTCCATG AAAGCCTTTC CCACATCTGC CTCAGCTAGA 
CAAGGCCGGT CCAGGATGCC TCCAGCTGCG AACAGCAGCC GCTACAGTAC ACGCAGTCGG 
CTCTACTGCG CTCCCATGCA GGCCATATCA GAAGGTGAAA CCGAAAACTC AGATGGAAGC 
CCACATGATG ACCGTTCCTC CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NHSA ( XM_021470044.1 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_021325719.1 Danio rerio Nance-Horan syndrome a (congenital cataracts and dental anomalies) (nhsa), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa151611
Clone ID Related Accession (Same CDS sequence)	XM_021470044.1
Accession Version	XM_021470044.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4641bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	Nance-Horan syndrome protein isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007134.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCGTTCT CCAAGCGCAT CGTGGAGCCC CAGTGGCTGT GCAGGCGCTC GGACACGGAG 
GACGAGCTGC TTCAGGATTT GTGCGCCTTC AGTAACGTGG CTCTGTCCCG GACCCTGCGC 
CAGCTGTCGG ACCTGGCCAA ACACGCCTGC TCGCTTTTCC AGGAGCTCGA GAATGAGCTG 
CTTTTCACGA ACCGGCGGGT GTGCGCGCTC CGGGACACCG TCAACCGTAT CCAGGAGAAC 
ACCGAGGCGC TCGACCCCAA GCAGGAGGCA GTGCCCTTGT CGAATCTGGA CATCGAGAGT 
AAATTGAGTG GACATTACCA GGCTCCATGG CACCTGCAGA GGAACGTGTT TCTGCCGTCC 
ACGAGGCCCG CGTGTGTGGA GGAGCTGCAC CGACACGCCA AACAGAGCCT GAGGGCCATG 
TACAGGGATC AGCAGCAGAG GCAGCAGGAG AGCGAGAGAG AGCGGCGGGT CACCATCTCC 
ATCTCCATGC TCCCGTCGGT GCCCTCATAC CCATCGCCCC GCGCCCACAG GAGACGCCAG 
CAGAGGGACA CACATCTTAG CACGGAGAGG ACGGAGAGAG ACACAGAGAC TCAGACAACA 
CAGACACAGT TTCAAAGCAC CCGGTCCTCC TCCCCTACTG AGTGTTGTCA TTTTTCCCCA 
TGGAGCAGAA AGGTTACTCC TCCAGGTGAT TCAGACTCAG AGCTGGTGGC TTTAGGCCAC 
TGGCCGAAGT GCCCGATCCC AAACGTGCCC TCAACGCTGG ACAAACAGAC CAACTGGGCA 
GGACTCTTAC CATTACCGAC CCCAGAAGAG AAAATGAAGA CCGACTCTCA AGTCATCAAC 
TCATGCATCG TCCCTATTAA CGTCACAGAA GAGGGCGCCA GCGCTTCTTC CCCCTCAGCG 
ATATCAGCTT TCCTAAGGGT TGGGTTCGAC AGAGAGGCCA GTGTACGCTG CTCGCTAGTG 
CATTCGCAGT CCGTGCTTCA GCGGCGGAGG AAGCTCAGGA GACGGAAAAC CATCACTGGC 
ATTCCCAGAC GAGTGCAGCA GGATTTGGAT TCTGACGAGT CTCCAGTGGC AAGAGAACGT 
ACTGTGATAG TACACACAAA TCCTGAAATT GGCCAGGAGG ATCTAACGCT CTCTCATCTC 
AAAACCAAGG ACTCTGGTTG CCAAACTGAA GAAATCCTAA TTGCTGGGGC TGCACCCTCA 
CGTAGACGGA TTAGAGCACA AAGAGGGCAG TGCATGACCG TGTCTCTGTC CCATTCGACA 
GGCAACATAT CTTCTCTGCC TGACAACTCT GACGCTATGT TCACTGCCTC GGCGGGCTCC 
CGTCTGCGTT CCCGAAGCCT TCCGCGAGAG GGAGGCCGAC TGGTGGATGA AGGTCATGAT 
GACAGCGATG AAGAGGAGGA TGAGGAAGAA TTGTCTCCGT TTGAGGCAGA GGACTTCCTA 
CCAGTTCCAG GGGAGCATAT ATTAAAAGAT GACGAGGAAA GCACTGATGA TCAGACGATC 
CCAGAAGGAC AGCATAATAG TTTGAAATTC AAGCAGCATG CTGGAAGTAC AGACCATGAA 
TGGTTGGAGA GGGGTCGATC CCGTCTGCCT CGGAAAGCTG ACATGGGTAG TTGTGAGATT 
TCCTCTAGTT CTGACACATT CAGTAGCCCT ATTCACTCCA TTTCCAATGC TGGTGTGCTG 
ACTAGCCAGA TGGACCACAA GGAGGATCAC CAGTCCTCAA GCGGCAACTG GAGTGGTAGT 
AGTTCAACAT GTCCCTCCCA GACGTCGGAA ACCATTCCAC CTGCAGCCTC ACCCCCACTC 
ACCGGATCAT CCCACTGTGA TTCAGAGCTA TCGCTGAATG CAGCTACCAA TACTAATGAT 
GATTCCACTG GCTTCATTAT TGATCCGTAT CCTGGGGACC GATTGCAAGG GCTCCGCAGC 
CATAGAACGG GTTCATTCAC TTCCACTGCA ACTGACATGC TTGACGATGC TGGAGTCAGC 
ACTGCTAGTG AGGGTGAATG GAGTTATCCC CAATACAGGC ATAACATACC TTGCCACCCT 
GACTTCAGTC CTGAGCACTC AAGGGCAGAG AACATACTCG AATGTCCCAG TTTTACTAGC 
ATAGCCACTT TTGAAAGTTT AACAGATAGG CCACCTTCTG AAAAGGCAGA CACCACCTCA 
CACTATTCTG TAGATGCCGA GGGCTACTAT ACCTCCATGC ACTTTGACTG TGGTCTTAAA 
GGTAGCAAAA GCTTCACATA TAACTATGCA TCTGTAGACC AGCAGCAGGC AGACTATGGA 
CATCAAACAA ACACACTTGG GAGACATAGT CTCTCTTTAA GGAAACCAAA GGTGAAGCCA 
TCCCCACCAA AACGCAGCTC ATCATTGAGG AAATTAAGCA GCCATGTTGG ACCTCCCACA 
GACAAGAATG AACCAAAGAT TGCAGGTGAT CAGAACAAAT CAATGTCTTC CGGGGAGAGA 
ACACATAACA TGGGATTAAA CGGAACCTCC TTGAGTCAGC TGGAGAGCGA GGAGCCTATG 
GGAGCATGGG GTGTTGAGAG TTCAGCCGAG ATTCCAGATG TTGCTTTGTT TGGCTCCACA 
GAAACGCATT CCTTCAAGGA TGAGGGAGCT GTCCAGTCAG ACTATGCAGA TCTCTGGCTT 
CTTAATGACT TAAAATCCAA CGATCCCTAC AGATCATTGT CAAATTCCAG CACAGCTACG 
GGTACCACTG TCATTGAATG CATTAAGTCA CAGGAGAGCT CAGAGTCCCA GACCTCACAG 
TCAGGTTCGA GAGCCACAAC TCCATCACTA CCATCAGTGG AGTGCGAGTT TAGGCTACCC 
TCACCAGAAA AGCTGGTAGG TCTAGCATCA CCTTCAAGTG GCTACTCAAG CCAATCAGAG 
ACACCAACAT CTTCTCTTCC ATCTGCTTTC TTTCCAAACG CTCACCCAAT GTCCCCTACT 
GGTGGCAAGA GGAAACCCAA AGTCCCAGAG AGGAAGTCCT CACTTGCTTC TTTGCAGCAG 
AACTCATCCA AAAGGGAACT TGAATTGCCT ATTATACCTC CCACCCACCT TGACCTAAGT 
GCCCTTCCCA ATGTCAGCAA GCCTTCGGTT CACAAAAACC AGATGCACGT CCTTCACCAC 
AGCAAGAAAA AAGCAGCAGA AACCAAGCGT GAGTGTCAAG CATCTTCAGT TTCAAGTGAT 
GATCTACCAG TTAGCGGTGC TTTAGCCATC ACTCCTACAA TACTTCGTTC GGTTCAGCTA 
CGAGCAGTTG GAAAATCTGG AGAAGGGAGT CATGATCAGC CTATAACTGA TACTGCCACT 
AGGCCTAAGT GTCCCACTGT GACTATCATC ACACCACCTA CTCACAACAA ATCCCACGAA 
AGTAGAAAAC CTCCACCCTA TAGACCTCTT CTCACAGAAC CCACCTCTCA GGACAATTGT 
GAATCACTGT TTACCCCAGT AAATGGACTA GCTGCTAAGA ATACTCCAAG CCATTTTGGT 
GCAAGAAATC GATATGACCG ATTAGCCCCA TCACCTCTTT GGAGCATGAC TGCTTTTAAG 
GCTCAGACTG AGCCGGTAAA CCTGGATGAA GTGATCTCTG AGAATTCTCA AGAAGCTGCA 
GAAGAGAAGA TCCCCTCAGA TTATTTATTT GCCCAGAGTA GCATGGAAGT ACAGCAAAAC 
CAGTCATTGA GATCTGAGCC AAACGACAGA AGCCCTGGTC AGAATGGTGT AGCAAAAAGG 
TCAGATAGTC TTGACCAAGT GCCTGATATT GTCAATCAAA GATCAGAGAC ATTGCAAGCT 
TACAAAATCA GTGGTGCTGG AGAAGAATCT GTAACATCAG GTGTTCCAAC CAGAAGTGCC 
TCACAAGAAT ATGTAGAGGA AGGGTCTACA CCAGACACAG AGGACTATTT TAGCAGAGAG 
TCCACACCAA GTGATCCATC TGTGTCCCCT CTGATAGACG AATCCAAAAC TGAAGATGAT 
AGCGTTTTCC TATCTCCAAG TAAAAGTCGC ACGACTGAAG ATCTCTTTGC CATGATTCAC 
AGGTCGAAAC GAAAAATGCT GGGCAGGAAA GATTCCTCTG AAACATTAGT GCGAGGCCGT 
TTAGCCGTTG TTTCTGGGAA TGGTCCTGCT ACAAATCCAA CAACCCCGCC AAGCACCCCA 
AGCATACCTG CAAGTCCATC TGCTCAAACG GGGGCTCAGA AAACACCGGG TCCAATCTAC 
AGGAGTGTCA AAAAGTCCAG TACCTCGAAT GAAGAGTTCA AACTGCTGCT TTTAAAAAAG 
GGAAGTCGCT CTGACTCTAG TTATCGAATG TCAGCTACAG AGATCCTAAA GAGCCCCATC 
GCGCCCAAAA GCCCAGGTGA GCTCTTGCTT GAGGGTTCTA GACCACTCGA GGAACCACTT 
TCCCCTATAC AACAGCAATT TCCAGAGGGA CCTCCAGAGC AAATACCTAG CCCTTTTCCA 
AAAACTTTTG CTGAGGGCTT CTCCATGAAA GCCTTTCCCA CATCTGCCTC AGCTAGACAA 
GGCCGGTCCA GGATGCCTCC AGCTGCGAAC AGCAGCCGCT ACAGTACACG CAGTCGGCTC 
TACTGCGCTC CCATGCAGGC CATATCAGAA GGTGAAACCG AAAACTCAGA TGGAAGCCCA 
CATGATGACC GTTCCTCCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021325719.1
CDS	210..4850
Translation	MPFSKRIVEPQWLCRRSDTEDELLQDLCAFSNVALSRTLRQLSDLAKHACSLFQELENELLFTNRRVCALRDTVNRIQENTEALDPKQEAVPLSNLDIESKLSGHYQAPWHLQRNVFLPSTRPACVEELHRHAKQSLRAMYRDQQQRQQESERERRVTISISMLPSVPSYPSPRAHRRRQQRDTHLSTERTERDTETQTTQTQFQSTRSSSPTECCHFSPWSRKVTPPGDSDSELVALGHWPKCPIPNVPSTLDKQTNWAGLLPLPTPEEKMKTDSQVINSCIVPINVTEEGASASSPSAISAFLRVGFDREASVRCSLVHSQSVLQRRRKLRRRKTITGIPRRVQQDLDSDESPVARERTVIVHTNPEIGQEDLTLSHLKTKDSGCQTEEILIAGAAPSRRRIRAQRGQCMTVSLSHSTGNISSLPDNSDAMFTASAGSRLRSRSLPREGGRLVDEGHDDSDEEEDEEELSPFEAEDFLPVPGEHILKDDEESTDDQTIPEGQHNSLKFKQHAGSTDHEWLERGRSRLPRKADMGSCEISSSSDTFSSPIHSISNAGVLTSQMDHKEDHQSSSGNWSGSSSTCPSQTSETIPPAASPPLTGSSHCDSELSLNAATNTNDDSTGFIIDPYPGDRLQGLRSHRTGSFTSTATDMLDDAGVSTASEGEWSYPQYRHNIPCHPDFSPEHSRAENILECPSFTSIATFESLTDRPPSEKADTTSHYSVDAEGYYTSMHFDCGLKGSKSFTYNYASVDQQQADYGHQTNTLGRHSLSLRKPKVKPSPPKRSSSLRKLSSHVGPPTDKNEPKIAGDQNKSMSSGERTHNMGLNGTSLSQLESEEPMGAWGVESSAEIPDVALFGSTETHSFKDEGAVQSDYADLWLLNDLKSNDPYRSLSNSSTATGTTVIECIKSQESSESQTSQSGSRATTPSLPSVECEFRLPSPEKLVGLASPSSGYSSQSETPTSSLPSAFFPNAHPMSPTGGKRKPKVPERKSSLASLQQNSSKRELELPIIPPTHLDLSALPNVSKPSVHKNQMHVLHHSKKKAAETKRECQASSVSSDDLPVSGALAITPTILRSVQLRAVGKSGEGSHDQPITDTATRPKCPTVTIITPPTHNKSHESRKPPPYRPLLTEPTSQDNCESLFTPVNGLAAKNTPSHFGARNRYDRLAPSPLWSMTAFKAQTEPVNLDEVISENSQEAAEEKIPSDYLFAQSSMEVQQNQSLRSEPNDRSPGQNGVAKRSDSLDQVPDIVNQRSETLQAYKISGAGEESVTSGVPTRSASQEYVEEGSTPDTEDYFSRESTPSDPSVSPLIDESKTEDDSVFLSPSKSRTTEDLFAMIHRSKRKMLGRKDSSETLVRGRLAVVSGNGPATNPTTPPSTPSIPASPSAQTGAQKTPGPIYRSVKKSSTSNEEFKLLLLKKGSRSDSSYRMSATEILKSPIAPKSPGELLLEGSRPLEEPLSPIQQQFPEGPPEQIPSPFPKTFAEGFSMKAFPTSASARQGRSRMPPAANSSRYSTRSRLYCAPMQAISEGETENSDGSPHDDRSS

Target ORF information:

RefSeq Version	XM_021470044.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio Nance-Horan syndrome a (congenital cataracts and dental anomalies) (nhsa), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021470044.1

ORF Insert Sequence:

ATGCCGTTCT CCAAGCGCAT CGTGGAGCCC CAGTGGCTGT GCAGGCGCTC GGACACGGAG 
GACGAGCTGC TTCAGGATTT GTGCGCCTTC AGTAACGTGG CTCTGTCCCG GACCCTGCGC 
CAGCTGTCGG ACCTGGCCAA ACACGCCTGC TCGCTTTTCC AGGAGCTCGA GAATGAGCTG 
CTTTTCACGA ACCGGCGGGT GTGCGCGCTC CGGGACACCG TCAACCGTAT CCAGGAGAAC 
ACCGAGGCGC TCGACCCCAA GCAGGAGGCA GTGCCCTTGT CGAATCTGGA CATCGAGAGT 
AAATTGAGTG GACATTACCA GGCTCCATGG CACCTGCAGA GGAACGTGTT TCTGCCGTCC 
ACGAGGCCCG CGTGTGTGGA GGAGCTGCAC CGACACGCCA AACAGAGCCT GAGGGCCATG 
TACAGGGATC AGCAGCAGAG GCAGCAGGAG AGCGAGAGAG AGCGGCGGGT CACCATCTCC 
ATCTCCATGC TCCCGTCGGT GCCCTCATAC CCATCGCCCC GCGCCCACAG GAGACGCCAG 
CAGAGGGACA CACATCTTAG CACGGAGAGG ACGGAGAGAG ACACAGAGAC TCAGACAACA 
CAGACACAGT TTCAAAGCAC CCGGTCCTCC TCCCCTACTG AGTGTTGTCA TTTTTCCCCA 
TGGAGCAGAA AGGTTACTCC TCCAGGTGAT TCAGACTCAG AGCTGGTGGC TTTAGGCCAC 
TGGCCGAAGT GCCCGATCCC AAACGTGCCC TCAACGCTGG ACAAACAGAC CAACTGGGCA 
GGACTCTTAC CATTACCGAC CCCAGAAGAG AAAATGAAGA CCGACTCTCA AGTCATCAAC 
TCATGCATCG TCCCTATTAA CGTCACAGAA GAGGGCGCCA GCGCTTCTTC CCCCTCAGCG 
ATATCAGCTT TCCTAAGGGT TGGGTTCGAC AGAGAGGCCA GTGTACGCTG CTCGCTAGTG 
CATTCGCAGT CCGTGCTTCA GCGGCGGAGG AAGCTCAGGA GACGGAAAAC CATCACTGGC 
ATTCCCAGAC GAGTGCAGCA GGATTTGGAT TCTGACGAGT CTCCAGTGGC AAGAGAACGT 
ACTGTGATAG TACACACAAA TCCTGAAATT GGCCAGGAGG ATCTAACGCT CTCTCATCTC 
AAAACCAAGG ACTCTGGTTG CCAAACTGAA GAAATCCTAA TTGCTGGGGC TGCACCCTCA 
CGTAGACGGA TTAGAGCACA AAGAGGGCAG TGCATGACCG TGTCTCTGTC CCATTCGACA 
GGCAACATAT CTTCTCTGCC TGACAACTCT GACGCTATGT TCACTGCCTC GGCGGGCTCC 
CGTCTGCGTT CCCGAAGCCT TCCGCGAGAG GGAGGCCGAC TGGTGGATGA AGGTCATGAT 
GACAGCGATG AAGAGGAGGA TGAGGAAGAA TTGTCTCCGT TTGAGGCAGA GGACTTCCTA 
CCAGTTCCAG GGGAGCATAT ATTAAAAGAT GACGAGGAAA GCACTGATGA TCAGACGATC 
CCAGAAGGAC AGCATAATAG TTTGAAATTC AAGCAGCATG CTGGAAGTAC AGACCATGAA 
TGGTTGGAGA GGGGTCGATC CCGTCTGCCT CGGAAAGCTG ACATGGGTAG TTGTGAGATT 
TCCTCTAGTT CTGACACATT CAGTAGCCCT ATTCACTCCA TTTCCAATGC TGGTGTGCTG 
ACTAGCCAGA TGGACCACAA GGAGGATCAC CAGTCCTCAA GCGGCAACTG GAGTGGTAGT 
AGTTCAACAT GTCCCTCCCA GACGTCGGAA ACCATTCCAC CTGCAGCCTC ACCCCCACTC 
ACCGGATCAT CCCACTGTGA TTCAGAGCTA TCGCTGAATG CAGCTACCAA TACTAATGAT 
GATTCCACTG GCTTCATTAT TGATCCGTAT CCTGGGGACC GATTGCAAGG GCTCCGCAGC 
CATAGAACGG GTTCATTCAC TTCCACTGCA ACTGACATGC TTGACGATGC TGGAGTCAGC 
ACTGCTAGTG AGGGTGAATG GAGTTATCCC CAATACAGGC ATAACATACC TTGCCACCCT 
GACTTCAGTC CTGAGCACTC AAGGGCAGAG AACATACTCG AATGTCCCAG TTTTACTAGC 
ATAGCCACTT TTGAAAGTTT AACAGATAGG CCACCTTCTG AAAAGGCAGA CACCACCTCA 
CACTATTCTG TAGATGCCGA GGGCTACTAT ACCTCCATGC ACTTTGACTG TGGTCTTAAA 
GGTAGCAAAA GCTTCACATA TAACTATGCA TCTGTAGACC AGCAGCAGGC AGACTATGGA 
CATCAAACAA ACACACTTGG GAGACATAGT CTCTCTTTAA GGAAACCAAA GGTGAAGCCA 
TCCCCACCAA AACGCAGCTC ATCATTGAGG AAATTAAGCA GCCATGTTGG ACCTCCCACA 
GACAAGAATG AACCAAAGAT TGCAGGTGAT CAGAACAAAT CAATGTCTTC CGGGGAGAGA 
ACACATAACA TGGGATTAAA CGGAACCTCC TTGAGTCAGC TGGAGAGCGA GGAGCCTATG 
GGAGCATGGG GTGTTGAGAG TTCAGCCGAG ATTCCAGATG TTGCTTTGTT TGGCTCCACA 
GAAACGCATT CCTTCAAGGA TGAGGGAGCT GTCCAGTCAG ACTATGCAGA TCTCTGGCTT 
CTTAATGACT TAAAATCCAA CGATCCCTAC AGATCATTGT CAAATTCCAG CACAGCTACG 
GGTACCACTG TCATTGAATG CATTAAGTCA CAGGAGAGCT CAGAGTCCCA GACCTCACAG 
TCAGGTTCGA GAGCCACAAC TCCATCACTA CCATCAGTGG AGTGCGAGTT TAGGCTACCC 
TCACCAGAAA AGCTGGTAGG TCTAGCATCA CCTTCAAGTG GCTACTCAAG CCAATCAGAG 
ACACCAACAT CTTCTCTTCC ATCTGCTTTC TTTCCAAACG CTCACCCAAT GTCCCCTACT 
GGTGGCAAGA GGAAACCCAA AGTCCCAGAG AGGAAGTCCT CACTTGCTTC TTTGCAGCAG 
AACTCATCCA AAAGGGAACT TGAATTGCCT ATTATACCTC CCACCCACCT TGACCTAAGT 
GCCCTTCCCA ATGTCAGCAA GCCTTCGGTT CACAAAAACC AGATGCACGT CCTTCACCAC 
AGCAAGAAAA AAGCAGCAGA AACCAAGCGT GAGTGTCAAG CATCTTCAGT TTCAAGTGAT 
GATCTACCAG TTAGCGGTGC TTTAGCCATC ACTCCTACAA TACTTCGTTC GGTTCAGCTA 
CGAGCAGTTG GAAAATCTGG AGAAGGGAGT CATGATCAGC CTATAACTGA TACTGCCACT 
AGGCCTAAGT GTCCCACTGT GACTATCATC ACACCACCTA CTCACAACAA ATCCCACGAA 
AGTAGAAAAC CTCCACCCTA TAGACCTCTT CTCACAGAAC CCACCTCTCA GGACAATTGT 
GAATCACTGT TTACCCCAGT AAATGGACTA GCTGCTAAGA ATACTCCAAG CCATTTTGGT 
GCAAGAAATC GATATGACCG ATTAGCCCCA TCACCTCTTT GGAGCATGAC TGCTTTTAAG 
GCTCAGACTG AGCCGGTAAA CCTGGATGAA GTGATCTCTG AGAATTCTCA AGAAGCTGCA 
GAAGAGAAGA TCCCCTCAGA TTATTTATTT GCCCAGAGTA GCATGGAAGT ACAGCAAAAC 
CAGTCATTGA GATCTGAGCC AAACGACAGA AGCCCTGGTC AGAATGGTGT AGCAAAAAGG 
TCAGATAGTC TTGACCAAGT GCCTGATATT GTCAATCAAA GATCAGAGAC ATTGCAAGCT 
TACAAAATCA GTGGTGCTGG AGAAGAATCT GTAACATCAG GTGTTCCAAC CAGAAGTGCC 
TCACAAGAAT ATGTAGAGGA AGGGTCTACA CCAGACACAG AGGACTATTT TAGCAGAGAG 
TCCACACCAA GTGATCCATC TGTGTCCCCT CTGATAGACG AATCCAAAAC TGAAGATGAT 
AGCGTTTTCC TATCTCCAAG TAAAAGTCGC ACGACTGAAG ATCTCTTTGC CATGATTCAC 
AGGTCGAAAC GAAAAATGCT GGGCAGGAAA GATTCCTCTG AAACATTAGT GCGAGGCCGT 
TTAGCCGTTG TTTCTGGGAA TGGTCCTGCT ACAAATCCAA CAACCCCGCC AAGCACCCCA 
AGCATACCTG CAAGTCCATC TGCTCAAACG GGGGCTCAGA AAACACCGGG TCCAATCTAC 
AGGAGTGTCA AAAAGTCCAG TACCTCGAAT GAAGAGTTCA AACTGCTGCT TTTAAAAAAG 
GGAAGTCGCT CTGACTCTAG TTATCGAATG TCAGCTACAG AGATCCTAAA GAGCCCCATC 
GCGCCCAAAA GCCCAGGTGA GCTCTTGCTT GAGGGTTCTA GACCACTCGA GGAACCACTT 
TCCCCTATAC AACAGCAATT TCCAGAGGGA CCTCCAGAGC AAATACCTAG CCCTTTTCCA 
AAAACTTTTG CTGAGGGCTT CTCCATGAAA GCCTTTCCCA CATCTGCCTC AGCTAGACAA 
GGCCGGTCCA GGATGCCTCC AGCTGCGAAC AGCAGCCGCT ACAGTACACG CAGTCGGCTC 
TACTGCGCTC CCATGCAGGC CATATCAGAA GGTGAAACCG AAAACTCAGA TGGAAGCCCA 
CATGATGACC GTTCCTCCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NHSA ( XM_021470045.1 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_021325720.1 Danio rerio Nance-Horan syndrome a (congenital cataracts and dental anomalies) (nhsa), transcript variant X3, mRNA.

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CloneID	ODa151612
Clone ID Related Accession (Same CDS sequence)	XM_021470045.1
Accession Version	XM_021470045.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4578bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	Nance-Horan syndrome protein isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007134.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCGTTCT CCAAGCGCAT CGTGGAGCCC CAGTGGCTGT GCAGGCGCTC GGACACGGAG 
GACGAGCTGC TTCAGGATTT GTGCGCCTTC AGTAACGTGG CTCTGTCCCG GACCCTGCGC 
CAGCTGTCGG ACCTGGCCAA ACACGCCTGC TCGCTTTTCC AGGAGCTCGA GAATGAGCTG 
CTTTTCACGA ACCGGCGGGT GTGCGCGCTC CGGGACACCG TCAACCGTAT CCAGGAGAAC 
ACCGAGGCGC TCGACCCCAA GCAGGAGGCA GTGCCCTTGT CGAATCTGGA CATCGAGAGT 
AAATTGAGTG GACATTACCA GGCTCCATGG CACCTGCAGA GGAACGTGTT TCTGCCGTCC 
ACGAGGCCCG CGTGTGTGGA GGAGCTGCAC CGACACGCCA AACAGAGCCT GAGGGCCATG 
TACAGGGATC AGCAGCAGAG GCAGCAGGAG AGCGAGAGAG AGCGGCGGGT CACCATCTCC 
ATCTCCATGC TCCCGTCGGT GCCCTCATAC CCATCGCCCC GCGCCCACAG GAGACGCCAG 
CAGAGGGACA CACATCTTAG CACGGAGAGG ACGGAGAGAG ACACAGAGAC TCAGACAACA 
CAGACACAGG TTACTCCTCC AGGTGATTCA GACTCAGAGC TGGTGGCTTT AGGCCACTGG 
CCGAAGTGCC CGATCCCAAA CGTGCCCTCA ACGCTGGACA AACAGACCAA CTGGGCAGGA 
CTCTTACCAT TACCGACCCC AGAAGAGAAA ATGAAGACCG ACTCTCAAGT CATCAACTCA 
TGCATCGTCC CTATTAACGT CACAGAAGAG GGCGCCAGCG CTTCTTCCCC CTCAGCGATA 
TCAGCTTTCC TAAGGGTTGG GTTCGACAGA GAGGCCAGTG TACGCTGCTC GCTAGTGCAT 
TCGCAGTCCG TGCTTCAGCG GCGGAGGAAG CTCAGGAGAC GGAAAACCAT CACTGGCATT 
CCCAGACGAG TGCAGCAGGA TTTGGATTCT GACGAGTCTC CAGTGGCAAG AGAACGTACT 
GTGATAGTAC ACACAAATCC TGAAATTGGC CAGGAGGATC TAACGCTCTC TCATCTCAAA 
ACCAAGGACT CTGGTTGCCA AACTGAAGAA ATCCTAATTG CTGGGGCTGC ACCCTCACGT 
AGACGGATTA GAGCACAAAG AGGGCAGTGC ATGACCGTGT CTCTGTCCCA TTCGACAGGC 
AACATATCTT CTCTGCCTGA CAACTCTGAC GCTATGTTCA CTGCCTCGGC GGGCTCCCGT 
CTGCGTTCCC GAAGCCTTCC GCGAGAGGGA GGCCGACTGG TGGATGAAGG TCATGATGAC 
AGCGATGAAG AGGAGGATGA GGAAGAATTG TCTCCGTTTG AGGCAGAGGA CTTCCTACCA 
GTTCCAGGGG AGCATATATT AAAAGATGAC GAGGAAAGCA CTGATGATCA GACGATCCCA 
GAAGGACAGC ATAATAGTTT GAAATTCAAG CAGCATGCTG GAAGTACAGA CCATGAATGG 
TTGGAGAGGG GTCGATCCCG TCTGCCTCGG AAAGCTGACA TGGGTAGTTG TGAGATTTCC 
TCTAGTTCTG ACACATTCAG TAGCCCTATT CACTCCATTT CCAATGCTGG TGTGCTGACT 
AGCCAGATGG ACCACAAGGA GGATCACCAG TCCTCAAGCG GCAACTGGAG TGGTAGTAGT 
TCAACATGTC CCTCCCAGAC GTCGGAAACC ATTCCACCTG CAGCCTCACC CCCACTCACC 
GGATCATCCC ACTGTGATTC AGAGCTATCG CTGAATGCAG CTACCAATAC TAATGATGAT 
TCCACTGGCT TCATTATTGA TCCGTATCCT GGGGACCGAT TGCAAGGGCT CCGCAGCCAT 
AGAACGGGTT CATTCACTTC CACTGCAACT GACATGCTTG ACGATGCTGG AGTCAGCACT 
GCTAGTGAGG GTGAATGGAG TTATCCCCAA TACAGGCATA ACATACCTTG CCACCCTGAC 
TTCAGTCCTG AGCACTCAAG GGCAGAGAAC ATACTCGAAT GTCCCAGTTT TACTAGCATA 
GCCACTTTTG AAAGTTTAAC AGATAGGCCA CCTTCTGAAA AGGCAGACAC CACCTCACAC 
TATTCTGTAG ATGCCGAGGG CTACTATACC TCCATGCACT TTGACTGTGG TCTTAAAGGT 
AGCAAAAGCT TCACATATAA CTATGCATCT GTAGACCAGC AGCAGGCAGA CTATGGACAT 
CAAACAAACA CACTTGGGAG ACATAGTCTC TCTTTAAGGA AACCAAAGGT GAAGCCATCC 
CCACCAAAAC GCAGCTCATC ATTGAGGAAA TTAAGCAGCC ATGTTGGACC TCCCACAGAC 
AAGAATGAAC CAAAGATTGC AGGTGATCAG AACAAATCAA TGTCTTCCGG GGAGAGAACA 
CATAACATGG GATTAAACGG AACCTCCTTG AGTCAGCTGG AGAGCGAGGA GCCTATGGGA 
GCATGGGGTG TTGAGAGTTC AGCCGAGATT CCAGATGTTG CTTTGTTTGG CTCCACAGAA 
ACGCATTCCT TCAAGGATGA GGGAGCTGTC CAGTCAGACT ATGCAGATCT CTGGCTTCTT 
AATGACTTAA AATCCAACGA TCCCTACAGA TCATTGTCAA ATTCCAGCAC AGCTACGGGT 
ACCACTGTCA TTGAATGCAT TAAGTCACAG GAGAGCTCAG AGTCCCAGAC CTCACAGTCA 
GGTTCGAGAG CCACAACTCC ATCACTACCA TCAGTGGAGT GCGAGTTTAG GCTACCCTCA 
CCAGAAAAGC TGGTAGGTCT AGCATCACCT TCAAGTGGCT ACTCAAGCCA ATCAGAGACA 
CCAACATCTT CTCTTCCATC TGCTTTCTTT CCAAACGCTC ACCCAATGTC CCCTACTGGT 
GGCAAGAGGA AACCCAAAGT CCCAGAGAGG AAGTCCTCAC TTGCTTCTTT GCAGCAGAAC 
TCATCCAAAA GGGAACTTGA ATTGCCTATT ATACCTCCCA CCCACCTTGA CCTAAGTGCC 
CTTCCCAATG TCAGCAAGCC TTCGGTTCAC AAAAACCAGA TGCACGTCCT TCACCACAGC 
AAGAAAAAAG CAGCAGAAAC CAAGCGTGAG TGTCAAGCAT CTTCAGTTTC AAGTGATGAT 
CTACCAGTTA GCGGTGCTTT AGCCATCACT CCTACAATAC TTCGTTCGGT TCAGCTACGA 
GCAGTTGGAA AATCTGGAGA AGGGAGTCAT GATCAGCCTA TAACTGATAC TGCCACTAGG 
CCTAAGTGTC CCACTGTGAC TATCATCACA CCACCTACTC ACAACAAATC CCACGAAAGT 
AGAAAACCTC CACCCTATAG ACCTCTTCTC ACAGAACCCA CCTCTCAGGA CAATTGTGAA 
TCACTGTTTA CCCCAGTAAA TGGACTAGCT GCTAAGAATA CTCCAAGCCA TTTTGGTGCA 
AGAAATCGAT ATGACCGATT AGCCCCATCA CCTCTTTGGA GCATGACTGC TTTTAAGGCT 
CAGACTGAGC CGGTAAACCT GGATGAAGTG ATCTCTGAGA ATTCTCAAGA AGCTGCAGAA 
GAGAAGATCC CCTCAGATTA TTTATTTGCC CAGAGTAGCA TGGAAGTACA GCAAAACCAG 
TCATTGAGAT CTGAGCCAAA CGACAGAAGC CCTGGTCAGA ATGGTGTAGC AAAAAGGTCA 
GATAGTCTTG ACCAAGTGCC TGATATTGTC AATCAAAGAT CAGAGACATT GCAAGCTTAC 
AAAATCAGTG GTGCTGGAGA AGAATCTGTA ACATCAGGTG TTCCAACCAG AAGTGCCTCA 
CAAGAATATG TAGAGGAAGG GTCTACACCA GACACAGAGG ACTATTTTAG CAGAGAGTCC 
ACACCAAGTG ATCCATCTGT GTCCCCTCTG ATAGACGAAT CCAAAACTGA AGATGATAGC 
GTTTTCCTAT CTCCAAGTAA AAGTCGCACG ACTGAAGATC TCTTTGCCAT GATTCACAGG 
TCGAAACGAA AAATGCTGGG CAGGAAAGAT TCCTCTGAAA CATTAGTGCG AGGCCGTTTA 
GCCGTTGTTT CTGGGAATGG TCCTGCTACA AATCCAACAA CCCCGCCAAG CACCCCAAGC 
ATACCTGCAA GTCCATCTGC TCAAACGGGG GCTCAGAAAA CACCGGGTCC AATCTACAGG 
AGTGTCAAAA AGTCCAGTAC CTCGAATGAA GAGTTCAAAC TGCTGCTTTT AAAAAAGGGA 
AGTCGCTCTG ACTCTAGTTA TCGAATGTCA GCTACAGAGA TCCTAAAGAG CCCCATCGCG 
CCCAAAAGCC CAGGTGAGCT CTTGCTTGAG GGTTCTAGAC CACTCGAGGA ACCACTTTCC 
CCTATACAAC AGCAATTTCC AGAGGGACCT CCAGAGCAAA TACCTAGCCC TTTTCCAAAA 
ACTTTTGCTG AGGGCTTCTC CATGAAAGCC TTTCCCACAT CTGCCTCAGC TAGACAAGGC 
CGGTCCAGGA TGCCTCCAGC TGCGAACAGC AGCCGCTACA GTACACGCAG TCGGCTCTAC 
TGCGCTCCCA TGCAGGCCAT ATCAGAAGGT GAAACCGAAA ACTCAGATGG AAGCCCACAT 
GATGACCGTT CCTCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021325720.1
CDS	210..4787
Translation	MPFSKRIVEPQWLCRRSDTEDELLQDLCAFSNVALSRTLRQLSDLAKHACSLFQELENELLFTNRRVCALRDTVNRIQENTEALDPKQEAVPLSNLDIESKLSGHYQAPWHLQRNVFLPSTRPACVEELHRHAKQSLRAMYRDQQQRQQESERERRVTISISMLPSVPSYPSPRAHRRRQQRDTHLSTERTERDTETQTTQTQVTPPGDSDSELVALGHWPKCPIPNVPSTLDKQTNWAGLLPLPTPEEKMKTDSQVINSCIVPINVTEEGASASSPSAISAFLRVGFDREASVRCSLVHSQSVLQRRRKLRRRKTITGIPRRVQQDLDSDESPVARERTVIVHTNPEIGQEDLTLSHLKTKDSGCQTEEILIAGAAPSRRRIRAQRGQCMTVSLSHSTGNISSLPDNSDAMFTASAGSRLRSRSLPREGGRLVDEGHDDSDEEEDEEELSPFEAEDFLPVPGEHILKDDEESTDDQTIPEGQHNSLKFKQHAGSTDHEWLERGRSRLPRKADMGSCEISSSSDTFSSPIHSISNAGVLTSQMDHKEDHQSSSGNWSGSSSTCPSQTSETIPPAASPPLTGSSHCDSELSLNAATNTNDDSTGFIIDPYPGDRLQGLRSHRTGSFTSTATDMLDDAGVSTASEGEWSYPQYRHNIPCHPDFSPEHSRAENILECPSFTSIATFESLTDRPPSEKADTTSHYSVDAEGYYTSMHFDCGLKGSKSFTYNYASVDQQQADYGHQTNTLGRHSLSLRKPKVKPSPPKRSSSLRKLSSHVGPPTDKNEPKIAGDQNKSMSSGERTHNMGLNGTSLSQLESEEPMGAWGVESSAEIPDVALFGSTETHSFKDEGAVQSDYADLWLLNDLKSNDPYRSLSNSSTATGTTVIECIKSQESSESQTSQSGSRATTPSLPSVECEFRLPSPEKLVGLASPSSGYSSQSETPTSSLPSAFFPNAHPMSPTGGKRKPKVPERKSSLASLQQNSSKRELELPIIPPTHLDLSALPNVSKPSVHKNQMHVLHHSKKKAAETKRECQASSVSSDDLPVSGALAITPTILRSVQLRAVGKSGEGSHDQPITDTATRPKCPTVTIITPPTHNKSHESRKPPPYRPLLTEPTSQDNCESLFTPVNGLAAKNTPSHFGARNRYDRLAPSPLWSMTAFKAQTEPVNLDEVISENSQEAAEEKIPSDYLFAQSSMEVQQNQSLRSEPNDRSPGQNGVAKRSDSLDQVPDIVNQRSETLQAYKISGAGEESVTSGVPTRSASQEYVEEGSTPDTEDYFSRESTPSDPSVSPLIDESKTEDDSVFLSPSKSRTTEDLFAMIHRSKRKMLGRKDSSETLVRGRLAVVSGNGPATNPTTPPSTPSIPASPSAQTGAQKTPGPIYRSVKKSSTSNEEFKLLLLKKGSRSDSSYRMSATEILKSPIAPKSPGELLLEGSRPLEEPLSPIQQQFPEGPPEQIPSPFPKTFAEGFSMKAFPTSASARQGRSRMPPAANSSRYSTRSRLYCAPMQAISEGETENSDGSPHDDRSS

Target ORF information:

RefSeq Version	XM_021470045.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio Nance-Horan syndrome a (congenital cataracts and dental anomalies) (nhsa), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021470045.1

ORF Insert Sequence:

ATGCCGTTCT CCAAGCGCAT CGTGGAGCCC CAGTGGCTGT GCAGGCGCTC GGACACGGAG 
GACGAGCTGC TTCAGGATTT GTGCGCCTTC AGTAACGTGG CTCTGTCCCG GACCCTGCGC 
CAGCTGTCGG ACCTGGCCAA ACACGCCTGC TCGCTTTTCC AGGAGCTCGA GAATGAGCTG 
CTTTTCACGA ACCGGCGGGT GTGCGCGCTC CGGGACACCG TCAACCGTAT CCAGGAGAAC 
ACCGAGGCGC TCGACCCCAA GCAGGAGGCA GTGCCCTTGT CGAATCTGGA CATCGAGAGT 
AAATTGAGTG GACATTACCA GGCTCCATGG CACCTGCAGA GGAACGTGTT TCTGCCGTCC 
ACGAGGCCCG CGTGTGTGGA GGAGCTGCAC CGACACGCCA AACAGAGCCT GAGGGCCATG 
TACAGGGATC AGCAGCAGAG GCAGCAGGAG AGCGAGAGAG AGCGGCGGGT CACCATCTCC 
ATCTCCATGC TCCCGTCGGT GCCCTCATAC CCATCGCCCC GCGCCCACAG GAGACGCCAG 
CAGAGGGACA CACATCTTAG CACGGAGAGG ACGGAGAGAG ACACAGAGAC TCAGACAACA 
CAGACACAGG TTACTCCTCC AGGTGATTCA GACTCAGAGC TGGTGGCTTT AGGCCACTGG 
CCGAAGTGCC CGATCCCAAA CGTGCCCTCA ACGCTGGACA AACAGACCAA CTGGGCAGGA 
CTCTTACCAT TACCGACCCC AGAAGAGAAA ATGAAGACCG ACTCTCAAGT CATCAACTCA 
TGCATCGTCC CTATTAACGT CACAGAAGAG GGCGCCAGCG CTTCTTCCCC CTCAGCGATA 
TCAGCTTTCC TAAGGGTTGG GTTCGACAGA GAGGCCAGTG TACGCTGCTC GCTAGTGCAT 
TCGCAGTCCG TGCTTCAGCG GCGGAGGAAG CTCAGGAGAC GGAAAACCAT CACTGGCATT 
CCCAGACGAG TGCAGCAGGA TTTGGATTCT GACGAGTCTC CAGTGGCAAG AGAACGTACT 
GTGATAGTAC ACACAAATCC TGAAATTGGC CAGGAGGATC TAACGCTCTC TCATCTCAAA 
ACCAAGGACT CTGGTTGCCA AACTGAAGAA ATCCTAATTG CTGGGGCTGC ACCCTCACGT 
AGACGGATTA GAGCACAAAG AGGGCAGTGC ATGACCGTGT CTCTGTCCCA TTCGACAGGC 
AACATATCTT CTCTGCCTGA CAACTCTGAC GCTATGTTCA CTGCCTCGGC GGGCTCCCGT 
CTGCGTTCCC GAAGCCTTCC GCGAGAGGGA GGCCGACTGG TGGATGAAGG TCATGATGAC 
AGCGATGAAG AGGAGGATGA GGAAGAATTG TCTCCGTTTG AGGCAGAGGA CTTCCTACCA 
GTTCCAGGGG AGCATATATT AAAAGATGAC GAGGAAAGCA CTGATGATCA GACGATCCCA 
GAAGGACAGC ATAATAGTTT GAAATTCAAG CAGCATGCTG GAAGTACAGA CCATGAATGG 
TTGGAGAGGG GTCGATCCCG TCTGCCTCGG AAAGCTGACA TGGGTAGTTG TGAGATTTCC 
TCTAGTTCTG ACACATTCAG TAGCCCTATT CACTCCATTT CCAATGCTGG TGTGCTGACT 
AGCCAGATGG ACCACAAGGA GGATCACCAG TCCTCAAGCG GCAACTGGAG TGGTAGTAGT 
TCAACATGTC CCTCCCAGAC GTCGGAAACC ATTCCACCTG CAGCCTCACC CCCACTCACC 
GGATCATCCC ACTGTGATTC AGAGCTATCG CTGAATGCAG CTACCAATAC TAATGATGAT 
TCCACTGGCT TCATTATTGA TCCGTATCCT GGGGACCGAT TGCAAGGGCT CCGCAGCCAT 
AGAACGGGTT CATTCACTTC CACTGCAACT GACATGCTTG ACGATGCTGG AGTCAGCACT 
GCTAGTGAGG GTGAATGGAG TTATCCCCAA TACAGGCATA ACATACCTTG CCACCCTGAC 
TTCAGTCCTG AGCACTCAAG GGCAGAGAAC ATACTCGAAT GTCCCAGTTT TACTAGCATA 
GCCACTTTTG AAAGTTTAAC AGATAGGCCA CCTTCTGAAA AGGCAGACAC CACCTCACAC 
TATTCTGTAG ATGCCGAGGG CTACTATACC TCCATGCACT TTGACTGTGG TCTTAAAGGT 
AGCAAAAGCT TCACATATAA CTATGCATCT GTAGACCAGC AGCAGGCAGA CTATGGACAT 
CAAACAAACA CACTTGGGAG ACATAGTCTC TCTTTAAGGA AACCAAAGGT GAAGCCATCC 
CCACCAAAAC GCAGCTCATC ATTGAGGAAA TTAAGCAGCC ATGTTGGACC TCCCACAGAC 
AAGAATGAAC CAAAGATTGC AGGTGATCAG AACAAATCAA TGTCTTCCGG GGAGAGAACA 
CATAACATGG GATTAAACGG AACCTCCTTG AGTCAGCTGG AGAGCGAGGA GCCTATGGGA 
GCATGGGGTG TTGAGAGTTC AGCCGAGATT CCAGATGTTG CTTTGTTTGG CTCCACAGAA 
ACGCATTCCT TCAAGGATGA GGGAGCTGTC CAGTCAGACT ATGCAGATCT CTGGCTTCTT 
AATGACTTAA AATCCAACGA TCCCTACAGA TCATTGTCAA ATTCCAGCAC AGCTACGGGT 
ACCACTGTCA TTGAATGCAT TAAGTCACAG GAGAGCTCAG AGTCCCAGAC CTCACAGTCA 
GGTTCGAGAG CCACAACTCC ATCACTACCA TCAGTGGAGT GCGAGTTTAG GCTACCCTCA 
CCAGAAAAGC TGGTAGGTCT AGCATCACCT TCAAGTGGCT ACTCAAGCCA ATCAGAGACA 
CCAACATCTT CTCTTCCATC TGCTTTCTTT CCAAACGCTC ACCCAATGTC CCCTACTGGT 
GGCAAGAGGA AACCCAAAGT CCCAGAGAGG AAGTCCTCAC TTGCTTCTTT GCAGCAGAAC 
TCATCCAAAA GGGAACTTGA ATTGCCTATT ATACCTCCCA CCCACCTTGA CCTAAGTGCC 
CTTCCCAATG TCAGCAAGCC TTCGGTTCAC AAAAACCAGA TGCACGTCCT TCACCACAGC 
AAGAAAAAAG CAGCAGAAAC CAAGCGTGAG TGTCAAGCAT CTTCAGTTTC AAGTGATGAT 
CTACCAGTTA GCGGTGCTTT AGCCATCACT CCTACAATAC TTCGTTCGGT TCAGCTACGA 
GCAGTTGGAA AATCTGGAGA AGGGAGTCAT GATCAGCCTA TAACTGATAC TGCCACTAGG 
CCTAAGTGTC CCACTGTGAC TATCATCACA CCACCTACTC ACAACAAATC CCACGAAAGT 
AGAAAACCTC CACCCTATAG ACCTCTTCTC ACAGAACCCA CCTCTCAGGA CAATTGTGAA 
TCACTGTTTA CCCCAGTAAA TGGACTAGCT GCTAAGAATA CTCCAAGCCA TTTTGGTGCA 
AGAAATCGAT ATGACCGATT AGCCCCATCA CCTCTTTGGA GCATGACTGC TTTTAAGGCT 
CAGACTGAGC CGGTAAACCT GGATGAAGTG ATCTCTGAGA ATTCTCAAGA AGCTGCAGAA 
GAGAAGATCC CCTCAGATTA TTTATTTGCC CAGAGTAGCA TGGAAGTACA GCAAAACCAG 
TCATTGAGAT CTGAGCCAAA CGACAGAAGC CCTGGTCAGA ATGGTGTAGC AAAAAGGTCA 
GATAGTCTTG ACCAAGTGCC TGATATTGTC AATCAAAGAT CAGAGACATT GCAAGCTTAC 
AAAATCAGTG GTGCTGGAGA AGAATCTGTA ACATCAGGTG TTCCAACCAG AAGTGCCTCA 
CAAGAATATG TAGAGGAAGG GTCTACACCA GACACAGAGG ACTATTTTAG CAGAGAGTCC 
ACACCAAGTG ATCCATCTGT GTCCCCTCTG ATAGACGAAT CCAAAACTGA AGATGATAGC 
GTTTTCCTAT CTCCAAGTAA AAGTCGCACG ACTGAAGATC TCTTTGCCAT GATTCACAGG 
TCGAAACGAA AAATGCTGGG CAGGAAAGAT TCCTCTGAAA CATTAGTGCG AGGCCGTTTA 
GCCGTTGTTT CTGGGAATGG TCCTGCTACA AATCCAACAA CCCCGCCAAG CACCCCAAGC 
ATACCTGCAA GTCCATCTGC TCAAACGGGG GCTCAGAAAA CACCGGGTCC AATCTACAGG 
AGTGTCAAAA AGTCCAGTAC CTCGAATGAA GAGTTCAAAC TGCTGCTTTT AAAAAAGGGA 
AGTCGCTCTG ACTCTAGTTA TCGAATGTCA GCTACAGAGA TCCTAAAGAG CCCCATCGCG 
CCCAAAAGCC CAGGTGAGCT CTTGCTTGAG GGTTCTAGAC CACTCGAGGA ACCACTTTCC 
CCTATACAAC AGCAATTTCC AGAGGGACCT CCAGAGCAAA TACCTAGCCC TTTTCCAAAA 
ACTTTTGCTG AGGGCTTCTC CATGAAAGCC TTTCCCACAT CTGCCTCAGC TAGACAAGGC 
CGGTCCAGGA TGCCTCCAGC TGCGAACAGC AGCCGCTACA GTACACGCAG TCGGCTCTAC 
TGCGCTCCCA TGCAGGCCAT ATCAGAAGGT GAAACCGAAA ACTCAGATGG AAGCCCACAT 
GATGACCGTT CCTCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NHSA ( XM_001919305.6 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_001919340.4 Danio rerio Nance-Horan syndrome a (congenital cataracts and dental anomalies) (nhsa), transcript variant X1, mRNA.

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CloneID	ODa28082
Clone ID Related Accession (Same CDS sequence)	XM_001919305.6 , XM_001919305.7
Accession Version	XM_001919305.6	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4593bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	Nance-Horan syndrome protein isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007134.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_001919305.5. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCGTTCT CCAAGCGCAT CGTGGAGCCC CAGTGGCTGT GCAGGCGCTC GGACACGGAG 
GACGAGCTGC TTCAGGATTT GTGCGCCTTC AGTAACGTGG CTCTGTCCCG GACCCTGCGC 
CAGCTGTCGG ACCTGGCCAA ACACGCCTGC TCGCTTTTCC AGGAGCTCGA GAATGAGCTG 
CTTTTCACGA ACCGGCGGGT GTGCGCGCTC CGGGACACCG TCAACCGTAT CCAGGAGAAC 
ACCGAGGCGC TCGACCCCAA GCAGGAGGCA GTGCCCTTGT CGAATCTGGA CATCGAGAGT 
AAATTGAGTG GACATTACCA GGCTCCATGG CACCTGCAGA GGAACGTGTT TCTGCCGTCC 
ACGAGGCCCG CGTGTGTGGA GGAGCTGCAC CGACACGCCA AACAGAGCCT GAGGGCCATG 
TACAGGGATC AGCAGCAGAG GCAGCAGGAG AGCGAGAGAG AGCGGCGGGT CACCATCTCC 
ATCTCCATGC TCCCGTCGGT GCCCTCATAC CCATCGCCCC GCGCCCACAG GAGACGCCAG 
CAGAGGGACA CACATCTTAG CACGGAGAGG ACGGAGAGAG ACACAGAGAC TCAGACAACA 
CAGACACAGT TTCAAAGCAC CCGGTCCTCC TCCCCTACTG AGTGTTGTCA TTTTTCCCCA 
TGGAGCAGAA AGGTTACTCC TCCAGGTGAT TCAGACTCAG AGCTGGTGGC TTTAGGCCAC 
TGGCCGAAGT GCCCGATCCC AAACGTGCCC TCAACGCTGG ACAAACAGAC CAACTGGGCA 
GGACTCTTAC CATTACCGAC CCCAGAAGAG AAAATGAAGA CCGACTCTCA AGTCATCAAC 
TCATGCATCG TCCCTATTAA CGTCACAGGG GTTGGGTTCG ACAGAGAGGC CAGTGTACGC 
TGCTCGCTAG TGCATTCGCA GTCCGTGCTT CAGCGGCGGA GGAAGCTCAG GAGACGGAAA 
ACCATCACTG GCATTCCCAG ACGAGTGCAG CAGGATTTGG ATTCTGACGA GTCTCCAGTG 
GCAAGAGAAC GTACTGTGAT AGTACACACA AATCCTGAAA TTGGCCAGGA GGATCTAACG 
CTCTCTCATC TCAAAACCAA GGACTCTGGT TGCCAAACTG AAGAAATCCT AATTGCTGGG 
GCTGCACCCT CACGTAGACG GATTAGAGCA CAAAGAGGGC AGTGCATGAC CGTGTCTCTG 
TCCCATTCGA CAGGCAACAT ATCTTCTCTG CCTGACAACT CTGACGCTAT GTTCACTGCC 
TCGGCGGGCT CCCGTCTGCG TTCCCGAAGC CTTCCGCGAG AGGGAGGCCG ACTGGTGGAT 
GAAGGTCATG ATGACAGCGA TGAAGAGGAG GATGAGGAAG AATTGTCTCC GTTTGAGGCA 
GAGGACTTCC TACCAGTTCC AGGGGAGCAT ATATTAAAAG ATGACGAGGA AAGCACTGAT 
GATCAGACGA TCCCAGAAGG ACAGCATAAT AGTTTGAAAT TCAAGCAGCA TGCTGGAAGT 
ACAGACCATG AATGGTTGGA GAGGGGTCGA TCCCGTCTGC CTCGGAAAGC TGACATGGGT 
AGTTGTGAGA TTTCCTCTAG TTCTGACACA TTCAGTAGCC CTATTCACTC CATTTCCAAT 
GCTGGTGTGC TGACTAGCCA GATGGACCAC AAGGAGGATC ACCAGTCCTC AAGCGGCAAC 
TGGAGTGGTA GTAGTTCAAC ATGTCCCTCC CAGACGTCGG AAACCATTCC ACCTGCAGCC 
TCACCCCCAC TCACCGGATC ATCCCACTGT GATTCAGAGC TATCGCTGAA TGCAGCTACC 
AATACTAATG ATGATTCCAC TGGCTTCATT ATTGATCCGT ATCCTGGGGA CCGATTGCAA 
GGGCTCCGCA GCCATAGAAC GGGTTCATTC ACTTCCACTG CAACTGACAT GCTTGACGAT 
GCTGGAGTCA GCACTGCTAG TGAGGGTGAA TGGAGTTATC CCCAATACAG GCATAACATA 
CCTTGCCACC CTGACTTCAG TCCTGAGCAC TCAAGGGCAG AGAACATACT CGAATGTCCC 
AGTTTTACTA GCATAGCCAC TTTTGAAAGT TTAACAGATA GGCCACCTTC TGAAAAGGCA 
GACACCACCT CACACTATTC TGTAGATGCC GAGGGCTACT ATACCTCCAT GCACTTTGAC 
TGTGGTCTTA AAGGTAGCAA AAGCTTCACA TATAACTATG CATCTGTAGA CCAGCAGCAG 
GCAGACTATG GACATCAAAC AAACACACTT GGGAGACATA GTCTCTCTTT AAGGAAACCA 
AAGGTGAAGC CATCCCCACC AAAACGCAGC TCATCATTGA GGAAATTAAG CAGCCATGTT 
GGACCTCCCA CAGACAAGAA TGAACCAAAG ATTGCAGGTG ATCAGAACAA ATCAATGTCT 
TCCGGGGAGA GAACACATAA CATGGGATTA AACGGAACCT CCTTGAGTCA GCTGGAGAGC 
GAGGAGCCTA TGGGAGCATG GGGTGTTGAG AGTTCAGCCG AGATTCCAGA TGTTGCTTTG 
TTTGGCTCCA CAGAAACGCA TTCCTTCAAG GATGAGGGAG CTGTCCAGTC AGACTATGCA 
GATCTCTGGC TTCTTAATGA CTTAAAATCC AACGATCCCT ACAGATCATT GTCAAATTCC 
AGCACAGCTA CGGGTACCAC TGTCATTGAA TGCATTAAGT CACAGGAGAG CTCAGAGTCC 
CAGACCTCAC AGTCAGGTTC GAGAGCCACA ACTCCATCAC TACCATCAGT GGAGTGCGAG 
TTTAGGCTAC CCTCACCAGA AAAGCTGGTA GGTCTAGCAT CACCTTCAAG TGGCTACTCA 
AGCCAATCAG AGACACCAAC ATCTTCTCTT CCATCTGCTT TCTTTCCAAA CGCTCACCCA 
ATGTCCCCTA CTGGTGGCAA GAGGAAACCC AAAGTCCCAG AGAGGAAGTC CTCACTTGCT 
TCTTTGCAGC AGAACTCATC CAAAAGGGAA CTTGAATTGC CTATTATACC TCCCACCCAC 
CTTGACCTAA GTGCCCTTCC CAATGTCAGC AAGCCTTCGG TTCACAAAAA CCAGATGCAC 
GTCCTTCACC ACAGCAAGAA AAAAGCAGCA GAAACCAAGC GTGAGTGTCA AGCATCTTCA 
GTTTCAAGTG ATGATCTACC AGTTAGCGGT GCTTTAGCCA TCACTCCTAC AATACTTCGT 
TCGGTTCAGC TACGAGCAGT TGGAAAATCT GGAGAAGGGA GTCATGATCA GCCTATAACT 
GATACTGCCA CTAGGCCTAA GTGTCCCACT GTGACTATCA TCACACCACC TACTCACAAC 
AAATCCCACG AAAGTAGAAA ACCTCCACCC TATAGACCTC TTCTCACAGA ACCCACCTCT 
CAGGACAATT GTGAATCACT GTTTACCCCA GTAAATGGAC TAGCTGCTAA GAATACTCCA 
AGCCATTTTG GTGCAAGAAA TCGATATGAC CGATTAGCCC CATCACCTCT TTGGAGCATG 
ACTGCTTTTA AGGCTCAGAC TGAGCCGGTA AACCTGGATG AAGTGATCTC TGAGAATTCT 
CAAGAAGCTG CAGAAGAGAA GATCCCCTCA GATTATTTAT TTGCCCAGAG TAGCATGGAA 
GTACAGCAAA ACCAGTCATT GAGATCTGAG CCAAACGACA GAAGCCCTGG TCAGAATGGT 
GTAGCAAAAA GGTCAGATAG TCTTGACCAA GTGCCTGATA TTGTCAATCA AAGATCAGAG 
ACATTGCAAG CTTACAAAAT CAGTGGTGCT GGAGAAGAAT CTGTAACATC AGGTGTTCCA 
ACCAGAAGTG CCTCACAAGA ATATGTAGAG GAAGGGTCTA CACCAGACAC AGAGGACTAT 
TTTAGCAGAG AGTCCACACC AAGTGATCCA TCTGTGTCCC CTCTGATAGA CGAATCCAAA 
ACTGAAGATG ATAGCGTTTT CCTATCTCCA AGTAAAAGTC GCACGACTGA AGATCTCTTT 
GCCATGATTC ACAGGTCGAA ACGAAAAATG CTGGGCAGGA AAGATTCCTC TGAAACATTA 
GTGCGAGGCC GTTTAGCCGT TGTTTCTGGG AATGGTCCTG CTACAAATCC AACAACCCCG 
CCAAGCACCC CAAGCATACC TGCAAGTCCA TCTGCTCAAA CGGGGGCTCA GAAAACACCG 
GGTCCAATCT ACAGGAGTGT CAAAAAGTCC AGTACCTCGA ATGAAGAGTT CAAACTGCTG 
CTTTTAAAAA AGGGAAGTCG CTCTGACTCT AGTTATCGAA TGTCAGCTAC AGAGATCCTA 
AAGAGCCCCA TCGCGCCCAA AAGCCCAGGT GAGCTCTTGC TTGAGGGTTC TAGACCACTC 
GAGGAACCAC TTTCCCCTAT ACAACAGCAA TTTCCAGAGG GACCTCCAGA GCAAATACCT 
AGCCCTTTTC CAAAAACTTT TGCTGAGGGC TTCTCCATGA AAGCCTTTCC CACATCTGCC 
TCAGCTAGAC AAGGCCGGTC CAGGATGCCT CCAGCTGCGA ACAGCAGCCG CTACAGTACA 
CGCAGTCGGC TCTACTGCGC TCCCATGCAG GCCATATCAG AAGGTGAAAC CGAAAACTCA 
GATGGAAGCC CACATGATGA CCGTTCCTCC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_001919340.4
CDS	188..4780
Translation	MPFSKRIVEPQWLCRRSDTEDELLQDLCAFSNVALSRTLRQLSDLAKHACSLFQELENELLFTNRRVCALRDTVNRIQENTEALDPKQEAVPLSNLDIESKLSGHYQAPWHLQRNVFLPSTRPACVEELHRHAKQSLRAMYRDQQQRQQESERERRVTISISMLPSVPSYPSPRAHRRRQQRDTHLSTERTERDTETQTTQTQFQSTRSSSPTECCHFSPWSRKVTPPGDSDSELVALGHWPKCPIPNVPSTLDKQTNWAGLLPLPTPEEKMKTDSQVINSCIVPINVTGVGFDREASVRCSLVHSQSVLQRRRKLRRRKTITGIPRRVQQDLDSDESPVARERTVIVHTNPEIGQEDLTLSHLKTKDSGCQTEEILIAGAAPSRRRIRAQRGQCMTVSLSHSTGNISSLPDNSDAMFTASAGSRLRSRSLPREGGRLVDEGHDDSDEEEDEEELSPFEAEDFLPVPGEHILKDDEESTDDQTIPEGQHNSLKFKQHAGSTDHEWLERGRSRLPRKADMGSCEISSSSDTFSSPIHSISNAGVLTSQMDHKEDHQSSSGNWSGSSSTCPSQTSETIPPAASPPLTGSSHCDSELSLNAATNTNDDSTGFIIDPYPGDRLQGLRSHRTGSFTSTATDMLDDAGVSTASEGEWSYPQYRHNIPCHPDFSPEHSRAENILECPSFTSIATFESLTDRPPSEKADTTSHYSVDAEGYYTSMHFDCGLKGSKSFTYNYASVDQQQADYGHQTNTLGRHSLSLRKPKVKPSPPKRSSSLRKLSSHVGPPTDKNEPKIAGDQNKSMSSGERTHNMGLNGTSLSQLESEEPMGAWGVESSAEIPDVALFGSTETHSFKDEGAVQSDYADLWLLNDLKSNDPYRSLSNSSTATGTTVIECIKSQESSESQTSQSGSRATTPSLPSVECEFRLPSPEKLVGLASPSSGYSSQSETPTSSLPSAFFPNAHPMSPTGGKRKPKVPERKSSLASLQQNSSKRELELPIIPPTHLDLSALPNVSKPSVHKNQMHVLHHSKKKAAETKRECQASSVSSDDLPVSGALAITPTILRSVQLRAVGKSGEGSHDQPITDTATRPKCPTVTIITPPTHNKSHESRKPPPYRPLLTEPTSQDNCESLFTPVNGLAAKNTPSHFGARNRYDRLAPSPLWSMTAFKAQTEPVNLDEVISENSQEAAEEKIPSDYLFAQSSMEVQQNQSLRSEPNDRSPGQNGVAKRSDSLDQVPDIVNQRSETLQAYKISGAGEESVTSGVPTRSASQEYVEEGSTPDTEDYFSRESTPSDPSVSPLIDESKTEDDSVFLSPSKSRTTEDLFAMIHRSKRKMLGRKDSSETLVRGRLAVVSGNGPATNPTTPPSTPSIPASPSAQTGAQKTPGPIYRSVKKSSTSNEEFKLLLLKKGSRSDSSYRMSATEILKSPIAPKSPGELLLEGSRPLEEPLSPIQQQFPEGPPEQIPSPFPKTFAEGFSMKAFPTSASARQGRSRMPPAANSSRYSTRSRLYCAPMQAISEGETENSDGSPHDDRSS

Target ORF information:

RefSeq Version	XM_001919305.6
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio Nance-Horan syndrome a (congenital cataracts and dental anomalies) (nhsa), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_001919305.6

ORF Insert Sequence:

ATGCCGTTCT CCAAGCGCAT CGTGGAGCCC CAGTGGCTGT GCAGGCGCTC GGACACGGAG 
GACGAGCTGC TTCAGGATTT GTGCGCCTTC AGTAACGTGG CTCTGTCCCG GACCCTGCGC 
CAGCTGTCGG ACCTGGCCAA ACACGCCTGC TCGCTTTTCC AGGAGCTCGA GAATGAGCTG 
CTTTTCACGA ACCGGCGGGT GTGCGCGCTC CGGGACACCG TCAACCGTAT CCAGGAGAAC 
ACCGAGGCGC TCGACCCCAA GCAGGAGGCA GTGCCCTTGT CGAATCTGGA CATCGAGAGT 
AAATTGAGTG GACATTACCA GGCTCCATGG CACCTGCAGA GGAACGTGTT TCTGCCGTCC 
ACGAGGCCCG CGTGTGTGGA GGAGCTGCAC CGACACGCCA AACAGAGCCT GAGGGCCATG 
TACAGGGATC AGCAGCAGAG GCAGCAGGAG AGCGAGAGAG AGCGGCGGGT CACCATCTCC 
ATCTCCATGC TCCCGTCGGT GCCCTCATAC CCATCGCCCC GCGCCCACAG GAGACGCCAG 
CAGAGGGACA CACATCTTAG CACGGAGAGG ACGGAGAGAG ACACAGAGAC TCAGACAACA 
CAGACACAGT TTCAAAGCAC CCGGTCCTCC TCCCCTACTG AGTGTTGTCA TTTTTCCCCA 
TGGAGCAGAA AGGTTACTCC TCCAGGTGAT TCAGACTCAG AGCTGGTGGC TTTAGGCCAC 
TGGCCGAAGT GCCCGATCCC AAACGTGCCC TCAACGCTGG ACAAACAGAC CAACTGGGCA 
GGACTCTTAC CATTACCGAC CCCAGAAGAG AAAATGAAGA CCGACTCTCA AGTCATCAAC 
TCATGCATCG TCCCTATTAA CGTCACAGGG GTTGGGTTCG ACAGAGAGGC CAGTGTACGC 
TGCTCGCTAG TGCATTCGCA GTCCGTGCTT CAGCGGCGGA GGAAGCTCAG GAGACGGAAA 
ACCATCACTG GCATTCCCAG ACGAGTGCAG CAGGATTTGG ATTCTGACGA GTCTCCAGTG 
GCAAGAGAAC GTACTGTGAT AGTACACACA AATCCTGAAA TTGGCCAGGA GGATCTAACG 
CTCTCTCATC TCAAAACCAA GGACTCTGGT TGCCAAACTG AAGAAATCCT AATTGCTGGG 
GCTGCACCCT CACGTAGACG GATTAGAGCA CAAAGAGGGC AGTGCATGAC CGTGTCTCTG 
TCCCATTCGA CAGGCAACAT ATCTTCTCTG CCTGACAACT CTGACGCTAT GTTCACTGCC 
TCGGCGGGCT CCCGTCTGCG TTCCCGAAGC CTTCCGCGAG AGGGAGGCCG ACTGGTGGAT 
GAAGGTCATG ATGACAGCGA TGAAGAGGAG GATGAGGAAG AATTGTCTCC GTTTGAGGCA 
GAGGACTTCC TACCAGTTCC AGGGGAGCAT ATATTAAAAG ATGACGAGGA AAGCACTGAT 
GATCAGACGA TCCCAGAAGG ACAGCATAAT AGTTTGAAAT TCAAGCAGCA TGCTGGAAGT 
ACAGACCATG AATGGTTGGA GAGGGGTCGA TCCCGTCTGC CTCGGAAAGC TGACATGGGT 
AGTTGTGAGA TTTCCTCTAG TTCTGACACA TTCAGTAGCC CTATTCACTC CATTTCCAAT 
GCTGGTGTGC TGACTAGCCA GATGGACCAC AAGGAGGATC ACCAGTCCTC AAGCGGCAAC 
TGGAGTGGTA GTAGTTCAAC ATGTCCCTCC CAGACGTCGG AAACCATTCC ACCTGCAGCC 
TCACCCCCAC TCACCGGATC ATCCCACTGT GATTCAGAGC TATCGCTGAA TGCAGCTACC 
AATACTAATG ATGATTCCAC TGGCTTCATT ATTGATCCGT ATCCTGGGGA CCGATTGCAA 
GGGCTCCGCA GCCATAGAAC GGGTTCATTC ACTTCCACTG CAACTGACAT GCTTGACGAT 
GCTGGAGTCA GCACTGCTAG TGAGGGTGAA TGGAGTTATC CCCAATACAG GCATAACATA 
CCTTGCCACC CTGACTTCAG TCCTGAGCAC TCAAGGGCAG AGAACATACT CGAATGTCCC 
AGTTTTACTA GCATAGCCAC TTTTGAAAGT TTAACAGATA GGCCACCTTC TGAAAAGGCA 
GACACCACCT CACACTATTC TGTAGATGCC GAGGGCTACT ATACCTCCAT GCACTTTGAC 
TGTGGTCTTA AAGGTAGCAA AAGCTTCACA TATAACTATG CATCTGTAGA CCAGCAGCAG 
GCAGACTATG GACATCAAAC AAACACACTT GGGAGACATA GTCTCTCTTT AAGGAAACCA 
AAGGTGAAGC CATCCCCACC AAAACGCAGC TCATCATTGA GGAAATTAAG CAGCCATGTT 
GGACCTCCCA CAGACAAGAA TGAACCAAAG ATTGCAGGTG ATCAGAACAA ATCAATGTCT 
TCCGGGGAGA GAACACATAA CATGGGATTA AACGGAACCT CCTTGAGTCA GCTGGAGAGC 
GAGGAGCCTA TGGGAGCATG GGGTGTTGAG AGTTCAGCCG AGATTCCAGA TGTTGCTTTG 
TTTGGCTCCA CAGAAACGCA TTCCTTCAAG GATGAGGGAG CTGTCCAGTC AGACTATGCA 
GATCTCTGGC TTCTTAATGA CTTAAAATCC AACGATCCCT ACAGATCATT GTCAAATTCC 
AGCACAGCTA CGGGTACCAC TGTCATTGAA TGCATTAAGT CACAGGAGAG CTCAGAGTCC 
CAGACCTCAC AGTCAGGTTC GAGAGCCACA ACTCCATCAC TACCATCAGT GGAGTGCGAG 
TTTAGGCTAC CCTCACCAGA AAAGCTGGTA GGTCTAGCAT CACCTTCAAG TGGCTACTCA 
AGCCAATCAG AGACACCAAC ATCTTCTCTT CCATCTGCTT TCTTTCCAAA CGCTCACCCA 
ATGTCCCCTA CTGGTGGCAA GAGGAAACCC AAAGTCCCAG AGAGGAAGTC CTCACTTGCT 
TCTTTGCAGC AGAACTCATC CAAAAGGGAA CTTGAATTGC CTATTATACC TCCCACCCAC 
CTTGACCTAA GTGCCCTTCC CAATGTCAGC AAGCCTTCGG TTCACAAAAA CCAGATGCAC 
GTCCTTCACC ACAGCAAGAA AAAAGCAGCA GAAACCAAGC GTGAGTGTCA AGCATCTTCA 
GTTTCAAGTG ATGATCTACC AGTTAGCGGT GCTTTAGCCA TCACTCCTAC AATACTTCGT 
TCGGTTCAGC TACGAGCAGT TGGAAAATCT GGAGAAGGGA GTCATGATCA GCCTATAACT 
GATACTGCCA CTAGGCCTAA GTGTCCCACT GTGACTATCA TCACACCACC TACTCACAAC 
AAATCCCACG AAAGTAGAAA ACCTCCACCC TATAGACCTC TTCTCACAGA ACCCACCTCT 
CAGGACAATT GTGAATCACT GTTTACCCCA GTAAATGGAC TAGCTGCTAA GAATACTCCA 
AGCCATTTTG GTGCAAGAAA TCGATATGAC CGATTAGCCC CATCACCTCT TTGGAGCATG 
ACTGCTTTTA AGGCTCAGAC TGAGCCGGTA AACCTGGATG AAGTGATCTC TGAGAATTCT 
CAAGAAGCTG CAGAAGAGAA GATCCCCTCA GATTATTTAT TTGCCCAGAG TAGCATGGAA 
GTACAGCAAA ACCAGTCATT GAGATCTGAG CCAAACGACA GAAGCCCTGG TCAGAATGGT 
GTAGCAAAAA GGTCAGATAG TCTTGACCAA GTGCCTGATA TTGTCAATCA AAGATCAGAG 
ACATTGCAAG CTTACAAAAT CAGTGGTGCT GGAGAAGAAT CTGTAACATC AGGTGTTCCA 
ACCAGAAGTG CCTCACAAGA ATATGTAGAG GAAGGGTCTA CACCAGACAC AGAGGACTAT 
TTTAGCAGAG AGTCCACACC AAGTGATCCA TCTGTGTCCC CTCTGATAGA CGAATCCAAA 
ACTGAAGATG ATAGCGTTTT CCTATCTCCA AGTAAAAGTC GCACGACTGA AGATCTCTTT 
GCCATGATTC ACAGGTCGAA ACGAAAAATG CTGGGCAGGA AAGATTCCTC TGAAACATTA 
GTGCGAGGCC GTTTAGCCGT TGTTTCTGGG AATGGTCCTG CTACAAATCC AACAACCCCG 
CCAAGCACCC CAAGCATACC TGCAAGTCCA TCTGCTCAAA CGGGGGCTCA GAAAACACCG 
GGTCCAATCT ACAGGAGTGT CAAAAAGTCC AGTACCTCGA ATGAAGAGTT CAAACTGCTG 
CTTTTAAAAA AGGGAAGTCG CTCTGACTCT AGTTATCGAA TGTCAGCTAC AGAGATCCTA 
AAGAGCCCCA TCGCGCCCAA AAGCCCAGGT GAGCTCTTGC TTGAGGGTTC TAGACCACTC 
GAGGAACCAC TTTCCCCTAT ACAACAGCAA TTTCCAGAGG GACCTCCAGA GCAAATACCT 
AGCCCTTTTC CAAAAACTTT TGCTGAGGGC TTCTCCATGA AAGCCTTTCC CACATCTGCC 
TCAGCTAGAC AAGGCCGGTC CAGGATGCCT CCAGCTGCGA ACAGCAGCCG CTACAGTACA 
CGCAGTCGGC TCTACTGCGC TCCCATGCAG GCCATATCAG AAGGTGAAAC CGAAAACTCA 
GATGGAAGCC CACATGATGA CCGTTCCTCC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NHSA ( XM_001919305.7 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_001919340.4 Danio rerio Nance-Horan syndrome a (congenital cataracts and dental anomalies) (nhsa), transcript variant X2, mRNA.

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CloneID	ODa28082
Clone ID Related Accession (Same CDS sequence)	XM_001919305.6 , XM_001919305.7
Accession Version	XM_001919305.7 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4593bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	Nance-Horan syndrome protein isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007134.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_001919305.6. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCGTTCT CCAAGCGCAT CGTGGAGCCC CAGTGGCTGT GCAGGCGCTC GGACACGGAG 
GACGAGCTGC TTCAGGATTT GTGCGCCTTC AGTAACGTGG CTCTGTCCCG GACCCTGCGC 
CAGCTGTCGG ACCTGGCCAA ACACGCCTGC TCGCTTTTCC AGGAGCTCGA GAATGAGCTG 
CTTTTCACGA ACCGGCGGGT GTGCGCGCTC CGGGACACCG TCAACCGTAT CCAGGAGAAC 
ACCGAGGCGC TCGACCCCAA GCAGGAGGCA GTGCCCTTGT CGAATCTGGA CATCGAGAGT 
AAATTGAGTG GACATTACCA GGCTCCATGG CACCTGCAGA GGAACGTGTT TCTGCCGTCC 
ACGAGGCCCG CGTGTGTGGA GGAGCTGCAC CGACACGCCA AACAGAGCCT GAGGGCCATG 
TACAGGGATC AGCAGCAGAG GCAGCAGGAG AGCGAGAGAG AGCGGCGGGT CACCATCTCC 
ATCTCCATGC TCCCGTCGGT GCCCTCATAC CCATCGCCCC GCGCCCACAG GAGACGCCAG 
CAGAGGGACA CACATCTTAG CACGGAGAGG ACGGAGAGAG ACACAGAGAC TCAGACAACA 
CAGACACAGT TTCAAAGCAC CCGGTCCTCC TCCCCTACTG AGTGTTGTCA TTTTTCCCCA 
TGGAGCAGAA AGGTTACTCC TCCAGGTGAT TCAGACTCAG AGCTGGTGGC TTTAGGCCAC 
TGGCCGAAGT GCCCGATCCC AAACGTGCCC TCAACGCTGG ACAAACAGAC CAACTGGGCA 
GGACTCTTAC CATTACCGAC CCCAGAAGAG AAAATGAAGA CCGACTCTCA AGTCATCAAC 
TCATGCATCG TCCCTATTAA CGTCACAGGG GTTGGGTTCG ACAGAGAGGC CAGTGTACGC 
TGCTCGCTAG TGCATTCGCA GTCCGTGCTT CAGCGGCGGA GGAAGCTCAG GAGACGGAAA 
ACCATCACTG GCATTCCCAG ACGAGTGCAG CAGGATTTGG ATTCTGACGA GTCTCCAGTG 
GCAAGAGAAC GTACTGTGAT AGTACACACA AATCCTGAAA TTGGCCAGGA GGATCTAACG 
CTCTCTCATC TCAAAACCAA GGACTCTGGT TGCCAAACTG AAGAAATCCT AATTGCTGGG 
GCTGCACCCT CACGTAGACG GATTAGAGCA CAAAGAGGGC AGTGCATGAC CGTGTCTCTG 
TCCCATTCGA CAGGCAACAT ATCTTCTCTG CCTGACAACT CTGACGCTAT GTTCACTGCC 
TCGGCGGGCT CCCGTCTGCG TTCCCGAAGC CTTCCGCGAG AGGGAGGCCG ACTGGTGGAT 
GAAGGTCATG ATGACAGCGA TGAAGAGGAG GATGAGGAAG AATTGTCTCC GTTTGAGGCA 
GAGGACTTCC TACCAGTTCC AGGGGAGCAT ATATTAAAAG ATGACGAGGA AAGCACTGAT 
GATCAGACGA TCCCAGAAGG ACAGCATAAT AGTTTGAAAT TCAAGCAGCA TGCTGGAAGT 
ACAGACCATG AATGGTTGGA GAGGGGTCGA TCCCGTCTGC CTCGGAAAGC TGACATGGGT 
AGTTGTGAGA TTTCCTCTAG TTCTGACACA TTCAGTAGCC CTATTCACTC CATTTCCAAT 
GCTGGTGTGC TGACTAGCCA GATGGACCAC AAGGAGGATC ACCAGTCCTC AAGCGGCAAC 
TGGAGTGGTA GTAGTTCAAC ATGTCCCTCC CAGACGTCGG AAACCATTCC ACCTGCAGCC 
TCACCCCCAC TCACCGGATC ATCCCACTGT GATTCAGAGC TATCGCTGAA TGCAGCTACC 
AATACTAATG ATGATTCCAC TGGCTTCATT ATTGATCCGT ATCCTGGGGA CCGATTGCAA 
GGGCTCCGCA GCCATAGAAC GGGTTCATTC ACTTCCACTG CAACTGACAT GCTTGACGAT 
GCTGGAGTCA GCACTGCTAG TGAGGGTGAA TGGAGTTATC CCCAATACAG GCATAACATA 
CCTTGCCACC CTGACTTCAG TCCTGAGCAC TCAAGGGCAG AGAACATACT CGAATGTCCC 
AGTTTTACTA GCATAGCCAC TTTTGAAAGT TTAACAGATA GGCCACCTTC TGAAAAGGCA 
GACACCACCT CACACTATTC TGTAGATGCC GAGGGCTACT ATACCTCCAT GCACTTTGAC 
TGTGGTCTTA AAGGTAGCAA AAGCTTCACA TATAACTATG CATCTGTAGA CCAGCAGCAG 
GCAGACTATG GACATCAAAC AAACACACTT GGGAGACATA GTCTCTCTTT AAGGAAACCA 
AAGGTGAAGC CATCCCCACC AAAACGCAGC TCATCATTGA GGAAATTAAG CAGCCATGTT 
GGACCTCCCA CAGACAAGAA TGAACCAAAG ATTGCAGGTG ATCAGAACAA ATCAATGTCT 
TCCGGGGAGA GAACACATAA CATGGGATTA AACGGAACCT CCTTGAGTCA GCTGGAGAGC 
GAGGAGCCTA TGGGAGCATG GGGTGTTGAG AGTTCAGCCG AGATTCCAGA TGTTGCTTTG 
TTTGGCTCCA CAGAAACGCA TTCCTTCAAG GATGAGGGAG CTGTCCAGTC AGACTATGCA 
GATCTCTGGC TTCTTAATGA CTTAAAATCC AACGATCCCT ACAGATCATT GTCAAATTCC 
AGCACAGCTA CGGGTACCAC TGTCATTGAA TGCATTAAGT CACAGGAGAG CTCAGAGTCC 
CAGACCTCAC AGTCAGGTTC GAGAGCCACA ACTCCATCAC TACCATCAGT GGAGTGCGAG 
TTTAGGCTAC CCTCACCAGA AAAGCTGGTA GGTCTAGCAT CACCTTCAAG TGGCTACTCA 
AGCCAATCAG AGACACCAAC ATCTTCTCTT CCATCTGCTT TCTTTCCAAA CGCTCACCCA 
ATGTCCCCTA CTGGTGGCAA GAGGAAACCC AAAGTCCCAG AGAGGAAGTC CTCACTTGCT 
TCTTTGCAGC AGAACTCATC CAAAAGGGAA CTTGAATTGC CTATTATACC TCCCACCCAC 
CTTGACCTAA GTGCCCTTCC CAATGTCAGC AAGCCTTCGG TTCACAAAAA CCAGATGCAC 
GTCCTTCACC ACAGCAAGAA AAAAGCAGCA GAAACCAAGC GTGAGTGTCA AGCATCTTCA 
GTTTCAAGTG ATGATCTACC AGTTAGCGGT GCTTTAGCCA TCACTCCTAC AATACTTCGT 
TCGGTTCAGC TACGAGCAGT TGGAAAATCT GGAGAAGGGA GTCATGATCA GCCTATAACT 
GATACTGCCA CTAGGCCTAA GTGTCCCACT GTGACTATCA TCACACCACC TACTCACAAC 
AAATCCCACG AAAGTAGAAA ACCTCCACCC TATAGACCTC TTCTCACAGA ACCCACCTCT 
CAGGACAATT GTGAATCACT GTTTACCCCA GTAAATGGAC TAGCTGCTAA GAATACTCCA 
AGCCATTTTG GTGCAAGAAA TCGATATGAC CGATTAGCCC CATCACCTCT TTGGAGCATG 
ACTGCTTTTA AGGCTCAGAC TGAGCCGGTA AACCTGGATG AAGTGATCTC TGAGAATTCT 
CAAGAAGCTG CAGAAGAGAA GATCCCCTCA GATTATTTAT TTGCCCAGAG TAGCATGGAA 
GTACAGCAAA ACCAGTCATT GAGATCTGAG CCAAACGACA GAAGCCCTGG TCAGAATGGT 
GTAGCAAAAA GGTCAGATAG TCTTGACCAA GTGCCTGATA TTGTCAATCA AAGATCAGAG 
ACATTGCAAG CTTACAAAAT CAGTGGTGCT GGAGAAGAAT CTGTAACATC AGGTGTTCCA 
ACCAGAAGTG CCTCACAAGA ATATGTAGAG GAAGGGTCTA CACCAGACAC AGAGGACTAT 
TTTAGCAGAG AGTCCACACC AAGTGATCCA TCTGTGTCCC CTCTGATAGA CGAATCCAAA 
ACTGAAGATG ATAGCGTTTT CCTATCTCCA AGTAAAAGTC GCACGACTGA AGATCTCTTT 
GCCATGATTC ACAGGTCGAA ACGAAAAATG CTGGGCAGGA AAGATTCCTC TGAAACATTA 
GTGCGAGGCC GTTTAGCCGT TGTTTCTGGG AATGGTCCTG CTACAAATCC AACAACCCCG 
CCAAGCACCC CAAGCATACC TGCAAGTCCA TCTGCTCAAA CGGGGGCTCA GAAAACACCG 
GGTCCAATCT ACAGGAGTGT CAAAAAGTCC AGTACCTCGA ATGAAGAGTT CAAACTGCTG 
CTTTTAAAAA AGGGAAGTCG CTCTGACTCT AGTTATCGAA TGTCAGCTAC AGAGATCCTA 
AAGAGCCCCA TCGCGCCCAA AAGCCCAGGT GAGCTCTTGC TTGAGGGTTC TAGACCACTC 
GAGGAACCAC TTTCCCCTAT ACAACAGCAA TTTCCAGAGG GACCTCCAGA GCAAATACCT 
AGCCCTTTTC CAAAAACTTT TGCTGAGGGC TTCTCCATGA AAGCCTTTCC CACATCTGCC 
TCAGCTAGAC AAGGCCGGTC CAGGATGCCT CCAGCTGCGA ACAGCAGCCG CTACAGTACA 
CGCAGTCGGC TCTACTGCGC TCCCATGCAG GCCATATCAG AAGGTGAAAC CGAAAACTCA 
GATGGAAGCC CACATGATGA CCGTTCCTCC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_001919340.4
CDS	210..4802
Translation	MPFSKRIVEPQWLCRRSDTEDELLQDLCAFSNVALSRTLRQLSDLAKHACSLFQELENELLFTNRRVCALRDTVNRIQENTEALDPKQEAVPLSNLDIESKLSGHYQAPWHLQRNVFLPSTRPACVEELHRHAKQSLRAMYRDQQQRQQESERERRVTISISMLPSVPSYPSPRAHRRRQQRDTHLSTERTERDTETQTTQTQFQSTRSSSPTECCHFSPWSRKVTPPGDSDSELVALGHWPKCPIPNVPSTLDKQTNWAGLLPLPTPEEKMKTDSQVINSCIVPINVTGVGFDREASVRCSLVHSQSVLQRRRKLRRRKTITGIPRRVQQDLDSDESPVARERTVIVHTNPEIGQEDLTLSHLKTKDSGCQTEEILIAGAAPSRRRIRAQRGQCMTVSLSHSTGNISSLPDNSDAMFTASAGSRLRSRSLPREGGRLVDEGHDDSDEEEDEEELSPFEAEDFLPVPGEHILKDDEESTDDQTIPEGQHNSLKFKQHAGSTDHEWLERGRSRLPRKADMGSCEISSSSDTFSSPIHSISNAGVLTSQMDHKEDHQSSSGNWSGSSSTCPSQTSETIPPAASPPLTGSSHCDSELSLNAATNTNDDSTGFIIDPYPGDRLQGLRSHRTGSFTSTATDMLDDAGVSTASEGEWSYPQYRHNIPCHPDFSPEHSRAENILECPSFTSIATFESLTDRPPSEKADTTSHYSVDAEGYYTSMHFDCGLKGSKSFTYNYASVDQQQADYGHQTNTLGRHSLSLRKPKVKPSPPKRSSSLRKLSSHVGPPTDKNEPKIAGDQNKSMSSGERTHNMGLNGTSLSQLESEEPMGAWGVESSAEIPDVALFGSTETHSFKDEGAVQSDYADLWLLNDLKSNDPYRSLSNSSTATGTTVIECIKSQESSESQTSQSGSRATTPSLPSVECEFRLPSPEKLVGLASPSSGYSSQSETPTSSLPSAFFPNAHPMSPTGGKRKPKVPERKSSLASLQQNSSKRELELPIIPPTHLDLSALPNVSKPSVHKNQMHVLHHSKKKAAETKRECQASSVSSDDLPVSGALAITPTILRSVQLRAVGKSGEGSHDQPITDTATRPKCPTVTIITPPTHNKSHESRKPPPYRPLLTEPTSQDNCESLFTPVNGLAAKNTPSHFGARNRYDRLAPSPLWSMTAFKAQTEPVNLDEVISENSQEAAEEKIPSDYLFAQSSMEVQQNQSLRSEPNDRSPGQNGVAKRSDSLDQVPDIVNQRSETLQAYKISGAGEESVTSGVPTRSASQEYVEEGSTPDTEDYFSRESTPSDPSVSPLIDESKTEDDSVFLSPSKSRTTEDLFAMIHRSKRKMLGRKDSSETLVRGRLAVVSGNGPATNPTTPPSTPSIPASPSAQTGAQKTPGPIYRSVKKSSTSNEEFKLLLLKKGSRSDSSYRMSATEILKSPIAPKSPGELLLEGSRPLEEPLSPIQQQFPEGPPEQIPSPFPKTFAEGFSMKAFPTSASARQGRSRMPPAANSSRYSTRSRLYCAPMQAISEGETENSDGSPHDDRSS

Target ORF information:

RefSeq Version	XM_001919305.7
Organism	Danio rerio(zebrafish)
Definition	Danio rerio Nance-Horan syndrome a (congenital cataracts and dental anomalies) (nhsa), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_001919305.7

ORF Insert Sequence:

ATGCCGTTCT CCAAGCGCAT CGTGGAGCCC CAGTGGCTGT GCAGGCGCTC GGACACGGAG 
GACGAGCTGC TTCAGGATTT GTGCGCCTTC AGTAACGTGG CTCTGTCCCG GACCCTGCGC 
CAGCTGTCGG ACCTGGCCAA ACACGCCTGC TCGCTTTTCC AGGAGCTCGA GAATGAGCTG 
CTTTTCACGA ACCGGCGGGT GTGCGCGCTC CGGGACACCG TCAACCGTAT CCAGGAGAAC 
ACCGAGGCGC TCGACCCCAA GCAGGAGGCA GTGCCCTTGT CGAATCTGGA CATCGAGAGT 
AAATTGAGTG GACATTACCA GGCTCCATGG CACCTGCAGA GGAACGTGTT TCTGCCGTCC 
ACGAGGCCCG CGTGTGTGGA GGAGCTGCAC CGACACGCCA AACAGAGCCT GAGGGCCATG 
TACAGGGATC AGCAGCAGAG GCAGCAGGAG AGCGAGAGAG AGCGGCGGGT CACCATCTCC 
ATCTCCATGC TCCCGTCGGT GCCCTCATAC CCATCGCCCC GCGCCCACAG GAGACGCCAG 
CAGAGGGACA CACATCTTAG CACGGAGAGG ACGGAGAGAG ACACAGAGAC TCAGACAACA 
CAGACACAGT TTCAAAGCAC CCGGTCCTCC TCCCCTACTG AGTGTTGTCA TTTTTCCCCA 
TGGAGCAGAA AGGTTACTCC TCCAGGTGAT TCAGACTCAG AGCTGGTGGC TTTAGGCCAC 
TGGCCGAAGT GCCCGATCCC AAACGTGCCC TCAACGCTGG ACAAACAGAC CAACTGGGCA 
GGACTCTTAC CATTACCGAC CCCAGAAGAG AAAATGAAGA CCGACTCTCA AGTCATCAAC 
TCATGCATCG TCCCTATTAA CGTCACAGGG GTTGGGTTCG ACAGAGAGGC CAGTGTACGC 
TGCTCGCTAG TGCATTCGCA GTCCGTGCTT CAGCGGCGGA GGAAGCTCAG GAGACGGAAA 
ACCATCACTG GCATTCCCAG ACGAGTGCAG CAGGATTTGG ATTCTGACGA GTCTCCAGTG 
GCAAGAGAAC GTACTGTGAT AGTACACACA AATCCTGAAA TTGGCCAGGA GGATCTAACG 
CTCTCTCATC TCAAAACCAA GGACTCTGGT TGCCAAACTG AAGAAATCCT AATTGCTGGG 
GCTGCACCCT CACGTAGACG GATTAGAGCA CAAAGAGGGC AGTGCATGAC CGTGTCTCTG 
TCCCATTCGA CAGGCAACAT ATCTTCTCTG CCTGACAACT CTGACGCTAT GTTCACTGCC 
TCGGCGGGCT CCCGTCTGCG TTCCCGAAGC CTTCCGCGAG AGGGAGGCCG ACTGGTGGAT 
GAAGGTCATG ATGACAGCGA TGAAGAGGAG GATGAGGAAG AATTGTCTCC GTTTGAGGCA 
GAGGACTTCC TACCAGTTCC AGGGGAGCAT ATATTAAAAG ATGACGAGGA AAGCACTGAT 
GATCAGACGA TCCCAGAAGG ACAGCATAAT AGTTTGAAAT TCAAGCAGCA TGCTGGAAGT 
ACAGACCATG AATGGTTGGA GAGGGGTCGA TCCCGTCTGC CTCGGAAAGC TGACATGGGT 
AGTTGTGAGA TTTCCTCTAG TTCTGACACA TTCAGTAGCC CTATTCACTC CATTTCCAAT 
GCTGGTGTGC TGACTAGCCA GATGGACCAC AAGGAGGATC ACCAGTCCTC AAGCGGCAAC 
TGGAGTGGTA GTAGTTCAAC ATGTCCCTCC CAGACGTCGG AAACCATTCC ACCTGCAGCC 
TCACCCCCAC TCACCGGATC ATCCCACTGT GATTCAGAGC TATCGCTGAA TGCAGCTACC 
AATACTAATG ATGATTCCAC TGGCTTCATT ATTGATCCGT ATCCTGGGGA CCGATTGCAA 
GGGCTCCGCA GCCATAGAAC GGGTTCATTC ACTTCCACTG CAACTGACAT GCTTGACGAT 
GCTGGAGTCA GCACTGCTAG TGAGGGTGAA TGGAGTTATC CCCAATACAG GCATAACATA 
CCTTGCCACC CTGACTTCAG TCCTGAGCAC TCAAGGGCAG AGAACATACT CGAATGTCCC 
AGTTTTACTA GCATAGCCAC TTTTGAAAGT TTAACAGATA GGCCACCTTC TGAAAAGGCA 
GACACCACCT CACACTATTC TGTAGATGCC GAGGGCTACT ATACCTCCAT GCACTTTGAC 
TGTGGTCTTA AAGGTAGCAA AAGCTTCACA TATAACTATG CATCTGTAGA CCAGCAGCAG 
GCAGACTATG GACATCAAAC AAACACACTT GGGAGACATA GTCTCTCTTT AAGGAAACCA 
AAGGTGAAGC CATCCCCACC AAAACGCAGC TCATCATTGA GGAAATTAAG CAGCCATGTT 
GGACCTCCCA CAGACAAGAA TGAACCAAAG ATTGCAGGTG ATCAGAACAA ATCAATGTCT 
TCCGGGGAGA GAACACATAA CATGGGATTA AACGGAACCT CCTTGAGTCA GCTGGAGAGC 
GAGGAGCCTA TGGGAGCATG GGGTGTTGAG AGTTCAGCCG AGATTCCAGA TGTTGCTTTG 
TTTGGCTCCA CAGAAACGCA TTCCTTCAAG GATGAGGGAG CTGTCCAGTC AGACTATGCA 
GATCTCTGGC TTCTTAATGA CTTAAAATCC AACGATCCCT ACAGATCATT GTCAAATTCC 
AGCACAGCTA CGGGTACCAC TGTCATTGAA TGCATTAAGT CACAGGAGAG CTCAGAGTCC 
CAGACCTCAC AGTCAGGTTC GAGAGCCACA ACTCCATCAC TACCATCAGT GGAGTGCGAG 
TTTAGGCTAC CCTCACCAGA AAAGCTGGTA GGTCTAGCAT CACCTTCAAG TGGCTACTCA 
AGCCAATCAG AGACACCAAC ATCTTCTCTT CCATCTGCTT TCTTTCCAAA CGCTCACCCA 
ATGTCCCCTA CTGGTGGCAA GAGGAAACCC AAAGTCCCAG AGAGGAAGTC CTCACTTGCT 
TCTTTGCAGC AGAACTCATC CAAAAGGGAA CTTGAATTGC CTATTATACC TCCCACCCAC 
CTTGACCTAA GTGCCCTTCC CAATGTCAGC AAGCCTTCGG TTCACAAAAA CCAGATGCAC 
GTCCTTCACC ACAGCAAGAA AAAAGCAGCA GAAACCAAGC GTGAGTGTCA AGCATCTTCA 
GTTTCAAGTG ATGATCTACC AGTTAGCGGT GCTTTAGCCA TCACTCCTAC AATACTTCGT 
TCGGTTCAGC TACGAGCAGT TGGAAAATCT GGAGAAGGGA GTCATGATCA GCCTATAACT 
GATACTGCCA CTAGGCCTAA GTGTCCCACT GTGACTATCA TCACACCACC TACTCACAAC 
AAATCCCACG AAAGTAGAAA ACCTCCACCC TATAGACCTC TTCTCACAGA ACCCACCTCT 
CAGGACAATT GTGAATCACT GTTTACCCCA GTAAATGGAC TAGCTGCTAA GAATACTCCA 
AGCCATTTTG GTGCAAGAAA TCGATATGAC CGATTAGCCC CATCACCTCT TTGGAGCATG 
ACTGCTTTTA AGGCTCAGAC TGAGCCGGTA AACCTGGATG AAGTGATCTC TGAGAATTCT 
CAAGAAGCTG CAGAAGAGAA GATCCCCTCA GATTATTTAT TTGCCCAGAG TAGCATGGAA 
GTACAGCAAA ACCAGTCATT GAGATCTGAG CCAAACGACA GAAGCCCTGG TCAGAATGGT 
GTAGCAAAAA GGTCAGATAG TCTTGACCAA GTGCCTGATA TTGTCAATCA AAGATCAGAG 
ACATTGCAAG CTTACAAAAT CAGTGGTGCT GGAGAAGAAT CTGTAACATC AGGTGTTCCA 
ACCAGAAGTG CCTCACAAGA ATATGTAGAG GAAGGGTCTA CACCAGACAC AGAGGACTAT 
TTTAGCAGAG AGTCCACACC AAGTGATCCA TCTGTGTCCC CTCTGATAGA CGAATCCAAA 
ACTGAAGATG ATAGCGTTTT CCTATCTCCA AGTAAAAGTC GCACGACTGA AGATCTCTTT 
GCCATGATTC ACAGGTCGAA ACGAAAAATG CTGGGCAGGA AAGATTCCTC TGAAACATTA 
GTGCGAGGCC GTTTAGCCGT TGTTTCTGGG AATGGTCCTG CTACAAATCC AACAACCCCG 
CCAAGCACCC CAAGCATACC TGCAAGTCCA TCTGCTCAAA CGGGGGCTCA GAAAACACCG 
GGTCCAATCT ACAGGAGTGT CAAAAAGTCC AGTACCTCGA ATGAAGAGTT CAAACTGCTG 
CTTTTAAAAA AGGGAAGTCG CTCTGACTCT AGTTATCGAA TGTCAGCTAC AGAGATCCTA 
AAGAGCCCCA TCGCGCCCAA AAGCCCAGGT GAGCTCTTGC TTGAGGGTTC TAGACCACTC 
GAGGAACCAC TTTCCCCTAT ACAACAGCAA TTTCCAGAGG GACCTCCAGA GCAAATACCT 
AGCCCTTTTC CAAAAACTTT TGCTGAGGGC TTCTCCATGA AAGCCTTTCC CACATCTGCC 
TCAGCTAGAC AAGGCCGGTC CAGGATGCCT CCAGCTGCGA ACAGCAGCCG CTACAGTACA 
CGCAGTCGGC TCTACTGCGC TCCCATGCAG GCCATATCAG AAGGTGAAAC CGAAAACTCA 
GATGGAAGCC CACATGATGA CCGTTCCTCC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NHSA ( XM_009297102.2 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_009295377.1 Danio rerio Nance-Horan syndrome a (congenital cataracts and dental anomalies) (nhsa), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ODa48870
Clone ID Related Accession (Same CDS sequence)	XM_009297102.2
Accession Version	XM_009297102.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4530bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	Nance-Horan syndrome protein isoform X2
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007134.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009297102.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCGTTCT CCAAGCGCAT CGTGGAGCCC CAGTGGCTGT GCAGGCGCTC GGACACGGAG 
GACGAGCTGC TTCAGGATTT GTGCGCCTTC AGTAACGTGG CTCTGTCCCG GACCCTGCGC 
CAGCTGTCGG ACCTGGCCAA ACACGCCTGC TCGCTTTTCC AGGAGCTCGA GAATGAGCTG 
CTTTTCACGA ACCGGCGGGT GTGCGCGCTC CGGGACACCG TCAACCGTAT CCAGGAGAAC 
ACCGAGGCGC TCGACCCCAA GCAGGAGGCA GTGCCCTTGT CGAATCTGGA CATCGAGAGT 
AAATTGAGTG GACATTACCA GGCTCCATGG CACCTGCAGA GGAACGTGTT TCTGCCGTCC 
ACGAGGCCCG CGTGTGTGGA GGAGCTGCAC CGACACGCCA AACAGAGCCT GAGGGCCATG 
TACAGGGATC AGCAGCAGAG GCAGCAGGAG AGCGAGAGAG AGCGGCGGGT CACCATCTCC 
ATCTCCATGC TCCCGTCGGT GCCCTCATAC CCATCGCCCC GCGCCCACAG GAGACGCCAG 
CAGAGGGACA CACATCTTAG CACGGAGAGG ACGGAGAGAG ACACAGAGAC TCAGACAACA 
CAGACACAGG TTACTCCTCC AGGTGATTCA GACTCAGAGC TGGTGGCTTT AGGCCACTGG 
CCGAAGTGCC CGATCCCAAA CGTGCCCTCA ACGCTGGACA AACAGACCAA CTGGGCAGGA 
CTCTTACCAT TACCGACCCC AGAAGAGAAA ATGAAGACCG ACTCTCAAGT CATCAACTCA 
TGCATCGTCC CTATTAACGT CACAGGGGTT GGGTTCGACA GAGAGGCCAG TGTACGCTGC 
TCGCTAGTGC ATTCGCAGTC CGTGCTTCAG CGGCGGAGGA AGCTCAGGAG ACGGAAAACC 
ATCACTGGCA TTCCCAGACG AGTGCAGCAG GATTTGGATT CTGACGAGTC TCCAGTGGCA 
AGAGAACGTA CTGTGATAGT ACACACAAAT CCTGAAATTG GCCAGGAGGA TCTAACGCTC 
TCTCATCTCA AAACCAAGGA CTCTGGTTGC CAAACTGAAG AAATCCTAAT TGCTGGGGCT 
GCACCCTCAC GTAGACGGAT TAGAGCACAA AGAGGGCAGT GCATGACCGT GTCTCTGTCC 
CATTCGACAG GCAACATATC TTCTCTGCCT GACAACTCTG ACGCTATGTT CACTGCCTCG 
GCGGGCTCCC GTCTGCGTTC CCGAAGCCTT CCGCGAGAGG GAGGCCGACT GGTGGATGAA 
GGTCATGATG ACAGCGATGA AGAGGAGGAT GAGGAAGAAT TGTCTCCGTT TGAGGCAGAG 
GACTTCCTAC CAGTTCCAGG GGAGCATATA TTAAAAGATG ACGAGGAAAG CACTGATGAT 
CAGACGATCC CAGAAGGACA GCATAATAGT TTGAAATTCA AGCAGCATGC TGGAAGTACA 
GACCATGAAT GGTTGGAGAG GGGTCGATCC CGTCTGCCTC GGAAAGCTGA CATGGGTAGT 
TGTGAGATTT CCTCTAGTTC TGACACATTC AGTAGCCCTA TTCACTCCAT TTCCAATGCT 
GGTGTGCTGA CTAGCCAGAT GGACCACAAG GAGGATCACC AGTCCTCAAG CGGCAACTGG 
AGTGGTAGTA GTTCAACATG TCCCTCCCAG ACGTCGGAAA CCATTCCACC TGCAGCCTCA 
CCCCCACTCA CCGGATCATC CCACTGTGAT TCAGAGCTAT CGCTGAATGC AGCTACCAAT 
ACTAATGATG ATTCCACTGG CTTCATTATT GATCCGTATC CTGGGGACCG ATTGCAAGGG 
CTCCGCAGCC ATAGAACGGG TTCATTCACT TCCACTGCAA CTGACATGCT TGACGATGCT 
GGAGTCAGCA CTGCTAGTGA GGGTGAATGG AGTTATCCCC AATACAGGCA TAACATACCT 
TGCCACCCTG ACTTCAGTCC TGAGCACTCA AGGGCAGAGA ACATACTCGA ATGTCCCAGT 
TTTACTAGCA TAGCCACTTT TGAAAGTTTA ACAGATAGGC CACCTTCTGA AAAGGCAGAC 
ACCACCTCAC ACTATTCTGT AGATGCCGAG GGCTACTATA CCTCCATGCA CTTTGACTGT 
GGTCTTAAAG GTAGCAAAAG CTTCACATAT AACTATGCAT CTGTAGACCA GCAGCAGGCA 
GACTATGGAC ATCAAACAAA CACACTTGGG AGACATAGTC TCTCTTTAAG GAAACCAAAG 
GTGAAGCCAT CCCCACCAAA ACGCAGCTCA TCATTGAGGA AATTAAGCAG CCATGTTGGA 
CCTCCCACAG ACAAGAATGA ACCAAAGATT GCAGGTGATC AGAACAAATC AATGTCTTCC 
GGGGAGAGAA CACATAACAT GGGATTAAAC GGAACCTCCT TGAGTCAGCT GGAGAGCGAG 
GAGCCTATGG GAGCATGGGG TGTTGAGAGT TCAGCCGAGA TTCCAGATGT TGCTTTGTTT 
GGCTCCACAG AAACGCATTC CTTCAAGGAT GAGGGAGCTG TCCAGTCAGA CTATGCAGAT 
CTCTGGCTTC TTAATGACTT AAAATCCAAC GATCCCTACA GATCATTGTC AAATTCCAGC 
ACAGCTACGG GTACCACTGT CATTGAATGC ATTAAGTCAC AGGAGAGCTC AGAGTCCCAG 
ACCTCACAGT CAGGTTCGAG AGCCACAACT CCATCACTAC CATCAGTGGA GTGCGAGTTT 
AGGCTACCCT CACCAGAAAA GCTGGTAGGT CTAGCATCAC CTTCAAGTGG CTACTCAAGC 
CAATCAGAGA CACCAACATC TTCTCTTCCA TCTGCTTTCT TTCCAAACGC TCACCCAATG 
TCCCCTACTG GTGGCAAGAG GAAACCCAAA GTCCCAGAGA GGAAGTCCTC ACTTGCTTCT 
TTGCAGCAGA ACTCATCCAA AAGGGAACTT GAATTGCCTA TTATACCTCC CACCCACCTT 
GACCTAAGTG CCCTTCCCAA TGTCAGCAAG CCTTCGGTTC ACAAAAACCA GATGCACGTC 
CTTCACCACA GCAAGAAAAA AGCAGCAGAA ACCAAGCGTG AGTGTCAAGC ATCTTCAGTT 
TCAAGTGATG ATCTACCAGT TAGCGGTGCT TTAGCCATCA CTCCTACAAT ACTTCGTTCG 
GTTCAGCTAC GAGCAGTTGG AAAATCTGGA GAAGGGAGTC ATGATCAGCC TATAACTGAT 
ACTGCCACTA GGCCTAAGTG TCCCACTGTG ACTATCATCA CACCACCTAC TCACAACAAA 
TCCCACGAAA GTAGAAAACC TCCACCCTAT AGACCTCTTC TCACAGAACC CACCTCTCAG 
GACAATTGTG AATCACTGTT TACCCCAGTA AATGGACTAG CTGCTAAGAA TACTCCAAGC 
CATTTTGGTG CAAGAAATCG ATATGACCGA TTAGCCCCAT CACCTCTTTG GAGCATGACT 
GCTTTTAAGG CTCAGACTGA GCCGGTAAAC CTGGATGAAG TGATCTCTGA GAATTCTCAA 
GAAGCTGCAG AAGAGAAGAT CCCCTCAGAT TATTTATTTG CCCAGAGTAG CATGGAAGTA 
CAGCAAAACC AGTCATTGAG ATCTGAGCCA AACGACAGAA GCCCTGGTCA GAATGGTGTA 
GCAAAAAGGT CAGATAGTCT TGACCAAGTG CCTGATATTG TCAATCAAAG ATCAGAGACA 
TTGCAAGCTT ACAAAATCAG TGGTGCTGGA GAAGAATCTG TAACATCAGG TGTTCCAACC 
AGAAGTGCCT CACAAGAATA TGTAGAGGAA GGGTCTACAC CAGACACAGA GGACTATTTT 
AGCAGAGAGT CCACACCAAG TGATCCATCT GTGTCCCCTC TGATAGACGA ATCCAAAACT 
GAAGATGATA GCGTTTTCCT ATCTCCAAGT AAAAGTCGCA CGACTGAAGA TCTCTTTGCC 
ATGATTCACA GGTCGAAACG AAAAATGCTG GGCAGGAAAG ATTCCTCTGA AACATTAGTG 
CGAGGCCGTT TAGCCGTTGT TTCTGGGAAT GGTCCTGCTA CAAATCCAAC AACCCCGCCA 
AGCACCCCAA GCATACCTGC AAGTCCATCT GCTCAAACGG GGGCTCAGAA AACACCGGGT 
CCAATCTACA GGAGTGTCAA AAAGTCCAGT ACCTCGAATG AAGAGTTCAA ACTGCTGCTT 
TTAAAAAAGG GAAGTCGCTC TGACTCTAGT TATCGAATGT CAGCTACAGA GATCCTAAAG 
AGCCCCATCG CGCCCAAAAG CCCAGGTGAG CTCTTGCTTG AGGGTTCTAG ACCACTCGAG 
GAACCACTTT CCCCTATACA ACAGCAATTT CCAGAGGGAC CTCCAGAGCA AATACCTAGC 
CCTTTTCCAA AAACTTTTGC TGAGGGCTTC TCCATGAAAG CCTTTCCCAC ATCTGCCTCA 
GCTAGACAAG GCCGGTCCAG GATGCCTCCA GCTGCGAACA GCAGCCGCTA CAGTACACGC 
AGTCGGCTCT ACTGCGCTCC CATGCAGGCC ATATCAGAAG GTGAAACCGA AAACTCAGAT 
GGAAGCCCAC ATGATGACCG TTCCTCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009295377.1
CDS	188..4717
Translation	MPFSKRIVEPQWLCRRSDTEDELLQDLCAFSNVALSRTLRQLSDLAKHACSLFQELENELLFTNRRVCALRDTVNRIQENTEALDPKQEAVPLSNLDIESKLSGHYQAPWHLQRNVFLPSTRPACVEELHRHAKQSLRAMYRDQQQRQQESERERRVTISISMLPSVPSYPSPRAHRRRQQRDTHLSTERTERDTETQTTQTQVTPPGDSDSELVALGHWPKCPIPNVPSTLDKQTNWAGLLPLPTPEEKMKTDSQVINSCIVPINVTGVGFDREASVRCSLVHSQSVLQRRRKLRRRKTITGIPRRVQQDLDSDESPVARERTVIVHTNPEIGQEDLTLSHLKTKDSGCQTEEILIAGAAPSRRRIRAQRGQCMTVSLSHSTGNISSLPDNSDAMFTASAGSRLRSRSLPREGGRLVDEGHDDSDEEEDEEELSPFEAEDFLPVPGEHILKDDEESTDDQTIPEGQHNSLKFKQHAGSTDHEWLERGRSRLPRKADMGSCEISSSSDTFSSPIHSISNAGVLTSQMDHKEDHQSSSGNWSGSSSTCPSQTSETIPPAASPPLTGSSHCDSELSLNAATNTNDDSTGFIIDPYPGDRLQGLRSHRTGSFTSTATDMLDDAGVSTASEGEWSYPQYRHNIPCHPDFSPEHSRAENILECPSFTSIATFESLTDRPPSEKADTTSHYSVDAEGYYTSMHFDCGLKGSKSFTYNYASVDQQQADYGHQTNTLGRHSLSLRKPKVKPSPPKRSSSLRKLSSHVGPPTDKNEPKIAGDQNKSMSSGERTHNMGLNGTSLSQLESEEPMGAWGVESSAEIPDVALFGSTETHSFKDEGAVQSDYADLWLLNDLKSNDPYRSLSNSSTATGTTVIECIKSQESSESQTSQSGSRATTPSLPSVECEFRLPSPEKLVGLASPSSGYSSQSETPTSSLPSAFFPNAHPMSPTGGKRKPKVPERKSSLASLQQNSSKRELELPIIPPTHLDLSALPNVSKPSVHKNQMHVLHHSKKKAAETKRECQASSVSSDDLPVSGALAITPTILRSVQLRAVGKSGEGSHDQPITDTATRPKCPTVTIITPPTHNKSHESRKPPPYRPLLTEPTSQDNCESLFTPVNGLAAKNTPSHFGARNRYDRLAPSPLWSMTAFKAQTEPVNLDEVISENSQEAAEEKIPSDYLFAQSSMEVQQNQSLRSEPNDRSPGQNGVAKRSDSLDQVPDIVNQRSETLQAYKISGAGEESVTSGVPTRSASQEYVEEGSTPDTEDYFSRESTPSDPSVSPLIDESKTEDDSVFLSPSKSRTTEDLFAMIHRSKRKMLGRKDSSETLVRGRLAVVSGNGPATNPTTPPSTPSIPASPSAQTGAQKTPGPIYRSVKKSSTSNEEFKLLLLKKGSRSDSSYRMSATEILKSPIAPKSPGELLLEGSRPLEEPLSPIQQQFPEGPPEQIPSPFPKTFAEGFSMKAFPTSASARQGRSRMPPAANSSRYSTRSRLYCAPMQAISEGETENSDGSPHDDRSS

Target ORF information:

RefSeq Version	XM_009297102.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio Nance-Horan syndrome a (congenital cataracts and dental anomalies) (nhsa), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009297102.2

ORF Insert Sequence:

ATGCCGTTCT CCAAGCGCAT CGTGGAGCCC CAGTGGCTGT GCAGGCGCTC GGACACGGAG 
GACGAGCTGC TTCAGGATTT GTGCGCCTTC AGTAACGTGG CTCTGTCCCG GACCCTGCGC 
CAGCTGTCGG ACCTGGCCAA ACACGCCTGC TCGCTTTTCC AGGAGCTCGA GAATGAGCTG 
CTTTTCACGA ACCGGCGGGT GTGCGCGCTC CGGGACACCG TCAACCGTAT CCAGGAGAAC 
ACCGAGGCGC TCGACCCCAA GCAGGAGGCA GTGCCCTTGT CGAATCTGGA CATCGAGAGT 
AAATTGAGTG GACATTACCA GGCTCCATGG CACCTGCAGA GGAACGTGTT TCTGCCGTCC 
ACGAGGCCCG CGTGTGTGGA GGAGCTGCAC CGACACGCCA AACAGAGCCT GAGGGCCATG 
TACAGGGATC AGCAGCAGAG GCAGCAGGAG AGCGAGAGAG AGCGGCGGGT CACCATCTCC 
ATCTCCATGC TCCCGTCGGT GCCCTCATAC CCATCGCCCC GCGCCCACAG GAGACGCCAG 
CAGAGGGACA CACATCTTAG CACGGAGAGG ACGGAGAGAG ACACAGAGAC TCAGACAACA 
CAGACACAGG TTACTCCTCC AGGTGATTCA GACTCAGAGC TGGTGGCTTT AGGCCACTGG 
CCGAAGTGCC CGATCCCAAA CGTGCCCTCA ACGCTGGACA AACAGACCAA CTGGGCAGGA 
CTCTTACCAT TACCGACCCC AGAAGAGAAA ATGAAGACCG ACTCTCAAGT CATCAACTCA 
TGCATCGTCC CTATTAACGT CACAGGGGTT GGGTTCGACA GAGAGGCCAG TGTACGCTGC 
TCGCTAGTGC ATTCGCAGTC CGTGCTTCAG CGGCGGAGGA AGCTCAGGAG ACGGAAAACC 
ATCACTGGCA TTCCCAGACG AGTGCAGCAG GATTTGGATT CTGACGAGTC TCCAGTGGCA 
AGAGAACGTA CTGTGATAGT ACACACAAAT CCTGAAATTG GCCAGGAGGA TCTAACGCTC 
TCTCATCTCA AAACCAAGGA CTCTGGTTGC CAAACTGAAG AAATCCTAAT TGCTGGGGCT 
GCACCCTCAC GTAGACGGAT TAGAGCACAA AGAGGGCAGT GCATGACCGT GTCTCTGTCC 
CATTCGACAG GCAACATATC TTCTCTGCCT GACAACTCTG ACGCTATGTT CACTGCCTCG 
GCGGGCTCCC GTCTGCGTTC CCGAAGCCTT CCGCGAGAGG GAGGCCGACT GGTGGATGAA 
GGTCATGATG ACAGCGATGA AGAGGAGGAT GAGGAAGAAT TGTCTCCGTT TGAGGCAGAG 
GACTTCCTAC CAGTTCCAGG GGAGCATATA TTAAAAGATG ACGAGGAAAG CACTGATGAT 
CAGACGATCC CAGAAGGACA GCATAATAGT TTGAAATTCA AGCAGCATGC TGGAAGTACA 
GACCATGAAT GGTTGGAGAG GGGTCGATCC CGTCTGCCTC GGAAAGCTGA CATGGGTAGT 
TGTGAGATTT CCTCTAGTTC TGACACATTC AGTAGCCCTA TTCACTCCAT TTCCAATGCT 
GGTGTGCTGA CTAGCCAGAT GGACCACAAG GAGGATCACC AGTCCTCAAG CGGCAACTGG 
AGTGGTAGTA GTTCAACATG TCCCTCCCAG ACGTCGGAAA CCATTCCACC TGCAGCCTCA 
CCCCCACTCA CCGGATCATC CCACTGTGAT TCAGAGCTAT CGCTGAATGC AGCTACCAAT 
ACTAATGATG ATTCCACTGG CTTCATTATT GATCCGTATC CTGGGGACCG ATTGCAAGGG 
CTCCGCAGCC ATAGAACGGG TTCATTCACT TCCACTGCAA CTGACATGCT TGACGATGCT 
GGAGTCAGCA CTGCTAGTGA GGGTGAATGG AGTTATCCCC AATACAGGCA TAACATACCT 
TGCCACCCTG ACTTCAGTCC TGAGCACTCA AGGGCAGAGA ACATACTCGA ATGTCCCAGT 
TTTACTAGCA TAGCCACTTT TGAAAGTTTA ACAGATAGGC CACCTTCTGA AAAGGCAGAC 
ACCACCTCAC ACTATTCTGT AGATGCCGAG GGCTACTATA CCTCCATGCA CTTTGACTGT 
GGTCTTAAAG GTAGCAAAAG CTTCACATAT AACTATGCAT CTGTAGACCA GCAGCAGGCA 
GACTATGGAC ATCAAACAAA CACACTTGGG AGACATAGTC TCTCTTTAAG GAAACCAAAG 
GTGAAGCCAT CCCCACCAAA ACGCAGCTCA TCATTGAGGA AATTAAGCAG CCATGTTGGA 
CCTCCCACAG ACAAGAATGA ACCAAAGATT GCAGGTGATC AGAACAAATC AATGTCTTCC 
GGGGAGAGAA CACATAACAT GGGATTAAAC GGAACCTCCT TGAGTCAGCT GGAGAGCGAG 
GAGCCTATGG GAGCATGGGG TGTTGAGAGT TCAGCCGAGA TTCCAGATGT TGCTTTGTTT 
GGCTCCACAG AAACGCATTC CTTCAAGGAT GAGGGAGCTG TCCAGTCAGA CTATGCAGAT 
CTCTGGCTTC TTAATGACTT AAAATCCAAC GATCCCTACA GATCATTGTC AAATTCCAGC 
ACAGCTACGG GTACCACTGT CATTGAATGC ATTAAGTCAC AGGAGAGCTC AGAGTCCCAG 
ACCTCACAGT CAGGTTCGAG AGCCACAACT CCATCACTAC CATCAGTGGA GTGCGAGTTT 
AGGCTACCCT CACCAGAAAA GCTGGTAGGT CTAGCATCAC CTTCAAGTGG CTACTCAAGC 
CAATCAGAGA CACCAACATC TTCTCTTCCA TCTGCTTTCT TTCCAAACGC TCACCCAATG 
TCCCCTACTG GTGGCAAGAG GAAACCCAAA GTCCCAGAGA GGAAGTCCTC ACTTGCTTCT 
TTGCAGCAGA ACTCATCCAA AAGGGAACTT GAATTGCCTA TTATACCTCC CACCCACCTT 
GACCTAAGTG CCCTTCCCAA TGTCAGCAAG CCTTCGGTTC ACAAAAACCA GATGCACGTC 
CTTCACCACA GCAAGAAAAA AGCAGCAGAA ACCAAGCGTG AGTGTCAAGC ATCTTCAGTT 
TCAAGTGATG ATCTACCAGT TAGCGGTGCT TTAGCCATCA CTCCTACAAT ACTTCGTTCG 
GTTCAGCTAC GAGCAGTTGG AAAATCTGGA GAAGGGAGTC ATGATCAGCC TATAACTGAT 
ACTGCCACTA GGCCTAAGTG TCCCACTGTG ACTATCATCA CACCACCTAC TCACAACAAA 
TCCCACGAAA GTAGAAAACC TCCACCCTAT AGACCTCTTC TCACAGAACC CACCTCTCAG 
GACAATTGTG AATCACTGTT TACCCCAGTA AATGGACTAG CTGCTAAGAA TACTCCAAGC 
CATTTTGGTG CAAGAAATCG ATATGACCGA TTAGCCCCAT CACCTCTTTG GAGCATGACT 
GCTTTTAAGG CTCAGACTGA GCCGGTAAAC CTGGATGAAG TGATCTCTGA GAATTCTCAA 
GAAGCTGCAG AAGAGAAGAT CCCCTCAGAT TATTTATTTG CCCAGAGTAG CATGGAAGTA 
CAGCAAAACC AGTCATTGAG ATCTGAGCCA AACGACAGAA GCCCTGGTCA GAATGGTGTA 
GCAAAAAGGT CAGATAGTCT TGACCAAGTG CCTGATATTG TCAATCAAAG ATCAGAGACA 
TTGCAAGCTT ACAAAATCAG TGGTGCTGGA GAAGAATCTG TAACATCAGG TGTTCCAACC 
AGAAGTGCCT CACAAGAATA TGTAGAGGAA GGGTCTACAC CAGACACAGA GGACTATTTT 
AGCAGAGAGT CCACACCAAG TGATCCATCT GTGTCCCCTC TGATAGACGA ATCCAAAACT 
GAAGATGATA GCGTTTTCCT ATCTCCAAGT AAAAGTCGCA CGACTGAAGA TCTCTTTGCC 
ATGATTCACA GGTCGAAACG AAAAATGCTG GGCAGGAAAG ATTCCTCTGA AACATTAGTG 
CGAGGCCGTT TAGCCGTTGT TTCTGGGAAT GGTCCTGCTA CAAATCCAAC AACCCCGCCA 
AGCACCCCAA GCATACCTGC AAGTCCATCT GCTCAAACGG GGGCTCAGAA AACACCGGGT 
CCAATCTACA GGAGTGTCAA AAAGTCCAGT ACCTCGAATG AAGAGTTCAA ACTGCTGCTT 
TTAAAAAAGG GAAGTCGCTC TGACTCTAGT TATCGAATGT CAGCTACAGA GATCCTAAAG 
AGCCCCATCG CGCCCAAAAG CCCAGGTGAG CTCTTGCTTG AGGGTTCTAG ACCACTCGAG 
GAACCACTTT CCCCTATACA ACAGCAATTT CCAGAGGGAC CTCCAGAGCA AATACCTAGC 
CCTTTTCCAA AAACTTTTGC TGAGGGCTTC TCCATGAAAG CCTTTCCCAC ATCTGCCTCA 
GCTAGACAAG GCCGGTCCAG GATGCCTCCA GCTGCGAACA GCAGCCGCTA CAGTACACGC 
AGTCGGCTCT ACTGCGCTCC CATGCAGGCC ATATCAGAAG GTGAAACCGA AAACTCAGAT 
GGAAGCCCAC ATGATGACCG TTCCTCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NHSA ( XM_009297103.2 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_009295378.1 Danio rerio Nance-Horan syndrome a (congenital cataracts and dental anomalies) (nhsa), transcript variant X3, mRNA.

Price & Availability↑

CloneID	ODa48871
Clone ID Related Accession (Same CDS sequence)	XM_009297103.2
Accession Version	XM_009297103.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4356bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	Nance-Horan syndrome protein isoform X3
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007134.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009297103.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTCTCG GATGCTGCAT ACCACTGAGG TCTAAAGCCT TGTCGAATCT GGACATCGAG 
AGTAAATTGA GTGGACATTA CCAGGCTCCA TGGCACCTGC AGAGGAACGT GTTTCTGCCG 
TCCACGAGGC CCGCGTGTGT GGAGGAGCTG CACCGACACG CCAAACAGAG CCTGAGGGCC 
ATGTACAGGG ATCAGCAGCA GAGGCAGCAG GAGAGCGAGA GAGAGCGGCG GGTCACCATC 
TCCATCTCCA TGCTCCCGTC GGTGCCCTCA TACCCATCGC CCCGCGCCCA CAGGAGACGC 
CAGCAGAGGG ACACACATCT TAGCACGGAG AGGACGGAGA GAGACACAGA GACTCAGACA 
ACACAGACAC AGTTTCAAAG CACCCGGTCC TCCTCCCCTA CTGAGTGTTG TCATTTTTCC 
CCATGGAGCA GAAAGGTTAC TCCTCCAGGT GATTCAGACT CAGAGCTGGT GGCTTTAGGC 
CACTGGCCGA AGTGCCCGAT CCCAAACGTG CCCTCAACGC TGGACAAACA GACCAACTGG 
GCAGGACTCT TACCATTACC GACCCCAGAA GAGAAAATGA AGACCGACTC TCAAGTCATC 
AACTCATGCA TCGTCCCTAT TAACGTCACA GGGGTTGGGT TCGACAGAGA GGCCAGTGTA 
CGCTGCTCGC TAGTGCATTC GCAGTCCGTG CTTCAGCGGC GGAGGAAGCT CAGGAGACGG 
AAAACCATCA CTGGCATTCC CAGACGAGTG CAGCAGGATT TGGATTCTGA CGAGTCTCCA 
GTGGCAAGAG AACGTACTGT GATAGTACAC ACAAATCCTG AAATTGGCCA GGAGGATCTA 
ACGCTCTCTC ATCTCAAAAC CAAGGACTCT GGTTGCCAAA CTGAAGAAAT CCTAATTGCT 
GGGGCTGCAC CCTCACGTAG ACGGATTAGA GCACAAAGAG GGCAGTGCAT GACCGTGTCT 
CTGTCCCATT CGACAGGCAA CATATCTTCT CTGCCTGACA ACTCTGACGC TATGTTCACT 
GCCTCGGCGG GCTCCCGTCT GCGTTCCCGA AGCCTTCCGC GAGAGGGAGG CCGACTGGTG 
GATGAAGGTC ATGATGACAG CGATGAAGAG GAGGATGAGG AAGAATTGTC TCCGTTTGAG 
GCAGAGGACT TCCTACCAGT TCCAGGGGAG CATATATTAA AAGATGACGA GGAAAGCACT 
GATGATCAGA CGATCCCAGA AGGACAGCAT AATAGTTTGA AATTCAAGCA GCATGCTGGA 
AGTACAGACC ATGAATGGTT GGAGAGGGGT CGATCCCGTC TGCCTCGGAA AGCTGACATG 
GGTAGTTGTG AGATTTCCTC TAGTTCTGAC ACATTCAGTA GCCCTATTCA CTCCATTTCC 
AATGCTGGTG TGCTGACTAG CCAGATGGAC CACAAGGAGG ATCACCAGTC CTCAAGCGGC 
AACTGGAGTG GTAGTAGTTC AACATGTCCC TCCCAGACGT CGGAAACCAT TCCACCTGCA 
GCCTCACCCC CACTCACCGG ATCATCCCAC TGTGATTCAG AGCTATCGCT GAATGCAGCT 
ACCAATACTA ATGATGATTC CACTGGCTTC ATTATTGATC CGTATCCTGG GGACCGATTG 
CAAGGGCTCC GCAGCCATAG AACGGGTTCA TTCACTTCCA CTGCAACTGA CATGCTTGAC 
GATGCTGGAG TCAGCACTGC TAGTGAGGGT GAATGGAGTT ATCCCCAATA CAGGCATAAC 
ATACCTTGCC ACCCTGACTT CAGTCCTGAG CACTCAAGGG CAGAGAACAT ACTCGAATGT 
CCCAGTTTTA CTAGCATAGC CACTTTTGAA AGTTTAACAG ATAGGCCACC TTCTGAAAAG 
GCAGACACCA CCTCACACTA TTCTGTAGAT GCCGAGGGCT ACTATACCTC CATGCACTTT 
GACTGTGGTC TTAAAGGTAG CAAAAGCTTC ACATATAACT ATGCATCTGT AGACCAGCAG 
CAGGCAGACT ATGGACATCA AACAAACACA CTTGGGAGAC ATAGTCTCTC TTTAAGGAAA 
CCAAAGGTGA AGCCATCCCC ACCAAAACGC AGCTCATCAT TGAGGAAATT AAGCAGCCAT 
GTTGGACCTC CCACAGACAA GAATGAACCA AAGATTGCAG GTGATCAGAA CAAATCAATG 
TCTTCCGGGG AGAGAACACA TAACATGGGA TTAAACGGAA CCTCCTTGAG TCAGCTGGAG 
AGCGAGGAGC CTATGGGAGC ATGGGGTGTT GAGAGTTCAG CCGAGATTCC AGATGTTGCT 
TTGTTTGGCT CCACAGAAAC GCATTCCTTC AAGGATGAGG GAGCTGTCCA GTCAGACTAT 
GCAGATCTCT GGCTTCTTAA TGACTTAAAA TCCAACGATC CCTACAGATC ATTGTCAAAT 
TCCAGCACAG CTACGGGTAC CACTGTCATT GAATGCATTA AGTCACAGGA GAGCTCAGAG 
TCCCAGACCT CACAGTCAGG TTCGAGAGCC ACAACTCCAT CACTACCATC AGTGGAGTGC 
GAGTTTAGGC TACCCTCACC AGAAAAGCTG GTAGGTCTAG CATCACCTTC AAGTGGCTAC 
TCAAGCCAAT CAGAGACACC AACATCTTCT CTTCCATCTG CTTTCTTTCC AAACGCTCAC 
CCAATGTCCC CTACTGGTGG CAAGAGGAAA CCCAAAGTCC CAGAGAGGAA GTCCTCACTT 
GCTTCTTTGC AGCAGAACTC ATCCAAAAGG GAACTTGAAT TGCCTATTAT ACCTCCCACC 
CACCTTGACC TAAGTGCCCT TCCCAATGTC AGCAAGCCTT CGGTTCACAA AAACCAGATG 
CACGTCCTTC ACCACAGCAA GAAAAAAGCA GCAGAAACCA AGCGTGAGTG TCAAGCATCT 
TCAGTTTCAA GTGATGATCT ACCAGTTAGC GGTGCTTTAG CCATCACTCC TACAATACTT 
CGTTCGGTTC AGCTACGAGC AGTTGGAAAA TCTGGAGAAG GGAGTCATGA TCAGCCTATA 
ACTGATACTG CCACTAGGCC TAAGTGTCCC ACTGTGACTA TCATCACACC ACCTACTCAC 
AACAAATCCC ACGAAAGTAG AAAACCTCCA CCCTATAGAC CTCTTCTCAC AGAACCCACC 
TCTCAGGACA ATTGTGAATC ACTGTTTACC CCAGTAAATG GACTAGCTGC TAAGAATACT 
CCAAGCCATT TTGGTGCAAG AAATCGATAT GACCGATTAG CCCCATCACC TCTTTGGAGC 
ATGACTGCTT TTAAGGCTCA GACTGAGCCG GTAAACCTGG ATGAAGTGAT CTCTGAGAAT 
TCTCAAGAAG CTGCAGAAGA GAAGATCCCC TCAGATTATT TATTTGCCCA GAGTAGCATG 
GAAGTACAGC AAAACCAGTC ATTGAGATCT GAGCCAAACG ACAGAAGCCC TGGTCAGAAT 
GGTGTAGCAA AAAGGTCAGA TAGTCTTGAC CAAGTGCCTG ATATTGTCAA TCAAAGATCA 
GAGACATTGC AAGCTTACAA AATCAGTGGT GCTGGAGAAG AATCTGTAAC ATCAGGTGTT 
CCAACCAGAA GTGCCTCACA AGAATATGTA GAGGAAGGGT CTACACCAGA CACAGAGGAC 
TATTTTAGCA GAGAGTCCAC ACCAAGTGAT CCATCTGTGT CCCCTCTGAT AGACGAATCC 
AAAACTGAAG ATGATAGCGT TTTCCTATCT CCAAGTAAAA GTCGCACGAC TGAAGATCTC 
TTTGCCATGA TTCACAGGTC GAAACGAAAA ATGCTGGGCA GGAAAGATTC CTCTGAAACA 
TTAGTGCGAG GCCGTTTAGC CGTTGTTTCT GGGAATGGTC CTGCTACAAA TCCAACAACC 
CCGCCAAGCA CCCCAAGCAT ACCTGCAAGT CCATCTGCTC AAACGGGGGC TCAGAAAACA 
CCGGGTCCAA TCTACAGGAG TGTCAAAAAG TCCAGTACCT CGAATGAAGA GTTCAAACTG 
CTGCTTTTAA AAAAGGGAAG TCGCTCTGAC TCTAGTTATC GAATGTCAGC TACAGAGATC 
CTAAAGAGCC CCATCGCGCC CAAAAGCCCA GGTGAGCTCT TGCTTGAGGG TTCTAGACCA 
CTCGAGGAAC CACTTTCCCC TATACAACAG CAATTTCCAG AGGGACCTCC AGAGCAAATA 
CCTAGCCCTT TTCCAAAAAC TTTTGCTGAG GGCTTCTCCA TGAAAGCCTT TCCCACATCT 
GCCTCAGCTA GACAAGGCCG GTCCAGGATG CCTCCAGCTG CGAACAGCAG CCGCTACAGT 
ACACGCAGTC GGCTCTACTG CGCTCCCATG CAGGCCATAT CAGAAGGTGA AACCGAAAAC 
TCAGATGGAA GCCCACATGA TGACCGTTCC TCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009295378.1
CDS	343..4698
Translation	MALGCCIPLRSKALSNLDIESKLSGHYQAPWHLQRNVFLPSTRPACVEELHRHAKQSLRAMYRDQQQRQQESERERRVTISISMLPSVPSYPSPRAHRRRQQRDTHLSTERTERDTETQTTQTQFQSTRSSSPTECCHFSPWSRKVTPPGDSDSELVALGHWPKCPIPNVPSTLDKQTNWAGLLPLPTPEEKMKTDSQVINSCIVPINVTGVGFDREASVRCSLVHSQSVLQRRRKLRRRKTITGIPRRVQQDLDSDESPVARERTVIVHTNPEIGQEDLTLSHLKTKDSGCQTEEILIAGAAPSRRRIRAQRGQCMTVSLSHSTGNISSLPDNSDAMFTASAGSRLRSRSLPREGGRLVDEGHDDSDEEEDEEELSPFEAEDFLPVPGEHILKDDEESTDDQTIPEGQHNSLKFKQHAGSTDHEWLERGRSRLPRKADMGSCEISSSSDTFSSPIHSISNAGVLTSQMDHKEDHQSSSGNWSGSSSTCPSQTSETIPPAASPPLTGSSHCDSELSLNAATNTNDDSTGFIIDPYPGDRLQGLRSHRTGSFTSTATDMLDDAGVSTASEGEWSYPQYRHNIPCHPDFSPEHSRAENILECPSFTSIATFESLTDRPPSEKADTTSHYSVDAEGYYTSMHFDCGLKGSKSFTYNYASVDQQQADYGHQTNTLGRHSLSLRKPKVKPSPPKRSSSLRKLSSHVGPPTDKNEPKIAGDQNKSMSSGERTHNMGLNGTSLSQLESEEPMGAWGVESSAEIPDVALFGSTETHSFKDEGAVQSDYADLWLLNDLKSNDPYRSLSNSSTATGTTVIECIKSQESSESQTSQSGSRATTPSLPSVECEFRLPSPEKLVGLASPSSGYSSQSETPTSSLPSAFFPNAHPMSPTGGKRKPKVPERKSSLASLQQNSSKRELELPIIPPTHLDLSALPNVSKPSVHKNQMHVLHHSKKKAAETKRECQASSVSSDDLPVSGALAITPTILRSVQLRAVGKSGEGSHDQPITDTATRPKCPTVTIITPPTHNKSHESRKPPPYRPLLTEPTSQDNCESLFTPVNGLAAKNTPSHFGARNRYDRLAPSPLWSMTAFKAQTEPVNLDEVISENSQEAAEEKIPSDYLFAQSSMEVQQNQSLRSEPNDRSPGQNGVAKRSDSLDQVPDIVNQRSETLQAYKISGAGEESVTSGVPTRSASQEYVEEGSTPDTEDYFSRESTPSDPSVSPLIDESKTEDDSVFLSPSKSRTTEDLFAMIHRSKRKMLGRKDSSETLVRGRLAVVSGNGPATNPTTPPSTPSIPASPSAQTGAQKTPGPIYRSVKKSSTSNEEFKLLLLKKGSRSDSSYRMSATEILKSPIAPKSPGELLLEGSRPLEEPLSPIQQQFPEGPPEQIPSPFPKTFAEGFSMKAFPTSASARQGRSRMPPAANSSRYSTRSRLYCAPMQAISEGETENSDGSPHDDRSS

Target ORF information:

RefSeq Version	XM_009297103.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio Nance-Horan syndrome a (congenital cataracts and dental anomalies) (nhsa), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009297103.2

ORF Insert Sequence:

ATGGCTCTCG GATGCTGCAT ACCACTGAGG TCTAAAGCCT TGTCGAATCT GGACATCGAG 
AGTAAATTGA GTGGACATTA CCAGGCTCCA TGGCACCTGC AGAGGAACGT GTTTCTGCCG 
TCCACGAGGC CCGCGTGTGT GGAGGAGCTG CACCGACACG CCAAACAGAG CCTGAGGGCC 
ATGTACAGGG ATCAGCAGCA GAGGCAGCAG GAGAGCGAGA GAGAGCGGCG GGTCACCATC 
TCCATCTCCA TGCTCCCGTC GGTGCCCTCA TACCCATCGC CCCGCGCCCA CAGGAGACGC 
CAGCAGAGGG ACACACATCT TAGCACGGAG AGGACGGAGA GAGACACAGA GACTCAGACA 
ACACAGACAC AGTTTCAAAG CACCCGGTCC TCCTCCCCTA CTGAGTGTTG TCATTTTTCC 
CCATGGAGCA GAAAGGTTAC TCCTCCAGGT GATTCAGACT CAGAGCTGGT GGCTTTAGGC 
CACTGGCCGA AGTGCCCGAT CCCAAACGTG CCCTCAACGC TGGACAAACA GACCAACTGG 
GCAGGACTCT TACCATTACC GACCCCAGAA GAGAAAATGA AGACCGACTC TCAAGTCATC 
AACTCATGCA TCGTCCCTAT TAACGTCACA GGGGTTGGGT TCGACAGAGA GGCCAGTGTA 
CGCTGCTCGC TAGTGCATTC GCAGTCCGTG CTTCAGCGGC GGAGGAAGCT CAGGAGACGG 
AAAACCATCA CTGGCATTCC CAGACGAGTG CAGCAGGATT TGGATTCTGA CGAGTCTCCA 
GTGGCAAGAG AACGTACTGT GATAGTACAC ACAAATCCTG AAATTGGCCA GGAGGATCTA 
ACGCTCTCTC ATCTCAAAAC CAAGGACTCT GGTTGCCAAA CTGAAGAAAT CCTAATTGCT 
GGGGCTGCAC CCTCACGTAG ACGGATTAGA GCACAAAGAG GGCAGTGCAT GACCGTGTCT 
CTGTCCCATT CGACAGGCAA CATATCTTCT CTGCCTGACA ACTCTGACGC TATGTTCACT 
GCCTCGGCGG GCTCCCGTCT GCGTTCCCGA AGCCTTCCGC GAGAGGGAGG CCGACTGGTG 
GATGAAGGTC ATGATGACAG CGATGAAGAG GAGGATGAGG AAGAATTGTC TCCGTTTGAG 
GCAGAGGACT TCCTACCAGT TCCAGGGGAG CATATATTAA AAGATGACGA GGAAAGCACT 
GATGATCAGA CGATCCCAGA AGGACAGCAT AATAGTTTGA AATTCAAGCA GCATGCTGGA 
AGTACAGACC ATGAATGGTT GGAGAGGGGT CGATCCCGTC TGCCTCGGAA AGCTGACATG 
GGTAGTTGTG AGATTTCCTC TAGTTCTGAC ACATTCAGTA GCCCTATTCA CTCCATTTCC 
AATGCTGGTG TGCTGACTAG CCAGATGGAC CACAAGGAGG ATCACCAGTC CTCAAGCGGC 
AACTGGAGTG GTAGTAGTTC AACATGTCCC TCCCAGACGT CGGAAACCAT TCCACCTGCA 
GCCTCACCCC CACTCACCGG ATCATCCCAC TGTGATTCAG AGCTATCGCT GAATGCAGCT 
ACCAATACTA ATGATGATTC CACTGGCTTC ATTATTGATC CGTATCCTGG GGACCGATTG 
CAAGGGCTCC GCAGCCATAG AACGGGTTCA TTCACTTCCA CTGCAACTGA CATGCTTGAC 
GATGCTGGAG TCAGCACTGC TAGTGAGGGT GAATGGAGTT ATCCCCAATA CAGGCATAAC 
ATACCTTGCC ACCCTGACTT CAGTCCTGAG CACTCAAGGG CAGAGAACAT ACTCGAATGT 
CCCAGTTTTA CTAGCATAGC CACTTTTGAA AGTTTAACAG ATAGGCCACC TTCTGAAAAG 
GCAGACACCA CCTCACACTA TTCTGTAGAT GCCGAGGGCT ACTATACCTC CATGCACTTT 
GACTGTGGTC TTAAAGGTAG CAAAAGCTTC ACATATAACT ATGCATCTGT AGACCAGCAG 
CAGGCAGACT ATGGACATCA AACAAACACA CTTGGGAGAC ATAGTCTCTC TTTAAGGAAA 
CCAAAGGTGA AGCCATCCCC ACCAAAACGC AGCTCATCAT TGAGGAAATT AAGCAGCCAT 
GTTGGACCTC CCACAGACAA GAATGAACCA AAGATTGCAG GTGATCAGAA CAAATCAATG 
TCTTCCGGGG AGAGAACACA TAACATGGGA TTAAACGGAA CCTCCTTGAG TCAGCTGGAG 
AGCGAGGAGC CTATGGGAGC ATGGGGTGTT GAGAGTTCAG CCGAGATTCC AGATGTTGCT 
TTGTTTGGCT CCACAGAAAC GCATTCCTTC AAGGATGAGG GAGCTGTCCA GTCAGACTAT 
GCAGATCTCT GGCTTCTTAA TGACTTAAAA TCCAACGATC CCTACAGATC ATTGTCAAAT 
TCCAGCACAG CTACGGGTAC CACTGTCATT GAATGCATTA AGTCACAGGA GAGCTCAGAG 
TCCCAGACCT CACAGTCAGG TTCGAGAGCC ACAACTCCAT CACTACCATC AGTGGAGTGC 
GAGTTTAGGC TACCCTCACC AGAAAAGCTG GTAGGTCTAG CATCACCTTC AAGTGGCTAC 
TCAAGCCAAT CAGAGACACC AACATCTTCT CTTCCATCTG CTTTCTTTCC AAACGCTCAC 
CCAATGTCCC CTACTGGTGG CAAGAGGAAA CCCAAAGTCC CAGAGAGGAA GTCCTCACTT 
GCTTCTTTGC AGCAGAACTC ATCCAAAAGG GAACTTGAAT TGCCTATTAT ACCTCCCACC 
CACCTTGACC TAAGTGCCCT TCCCAATGTC AGCAAGCCTT CGGTTCACAA AAACCAGATG 
CACGTCCTTC ACCACAGCAA GAAAAAAGCA GCAGAAACCA AGCGTGAGTG TCAAGCATCT 
TCAGTTTCAA GTGATGATCT ACCAGTTAGC GGTGCTTTAG CCATCACTCC TACAATACTT 
CGTTCGGTTC AGCTACGAGC AGTTGGAAAA TCTGGAGAAG GGAGTCATGA TCAGCCTATA 
ACTGATACTG CCACTAGGCC TAAGTGTCCC ACTGTGACTA TCATCACACC ACCTACTCAC 
AACAAATCCC ACGAAAGTAG AAAACCTCCA CCCTATAGAC CTCTTCTCAC AGAACCCACC 
TCTCAGGACA ATTGTGAATC ACTGTTTACC CCAGTAAATG GACTAGCTGC TAAGAATACT 
CCAAGCCATT TTGGTGCAAG AAATCGATAT GACCGATTAG CCCCATCACC TCTTTGGAGC 
ATGACTGCTT TTAAGGCTCA GACTGAGCCG GTAAACCTGG ATGAAGTGAT CTCTGAGAAT 
TCTCAAGAAG CTGCAGAAGA GAAGATCCCC TCAGATTATT TATTTGCCCA GAGTAGCATG 
GAAGTACAGC AAAACCAGTC ATTGAGATCT GAGCCAAACG ACAGAAGCCC TGGTCAGAAT 
GGTGTAGCAA AAAGGTCAGA TAGTCTTGAC CAAGTGCCTG ATATTGTCAA TCAAAGATCA 
GAGACATTGC AAGCTTACAA AATCAGTGGT GCTGGAGAAG AATCTGTAAC ATCAGGTGTT 
CCAACCAGAA GTGCCTCACA AGAATATGTA GAGGAAGGGT CTACACCAGA CACAGAGGAC 
TATTTTAGCA GAGAGTCCAC ACCAAGTGAT CCATCTGTGT CCCCTCTGAT AGACGAATCC 
AAAACTGAAG ATGATAGCGT TTTCCTATCT CCAAGTAAAA GTCGCACGAC TGAAGATCTC 
TTTGCCATGA TTCACAGGTC GAAACGAAAA ATGCTGGGCA GGAAAGATTC CTCTGAAACA 
TTAGTGCGAG GCCGTTTAGC CGTTGTTTCT GGGAATGGTC CTGCTACAAA TCCAACAACC 
CCGCCAAGCA CCCCAAGCAT ACCTGCAAGT CCATCTGCTC AAACGGGGGC TCAGAAAACA 
CCGGGTCCAA TCTACAGGAG TGTCAAAAAG TCCAGTACCT CGAATGAAGA GTTCAAACTG 
CTGCTTTTAA AAAAGGGAAG TCGCTCTGAC TCTAGTTATC GAATGTCAGC TACAGAGATC 
CTAAAGAGCC CCATCGCGCC CAAAAGCCCA GGTGAGCTCT TGCTTGAGGG TTCTAGACCA 
CTCGAGGAAC CACTTTCCCC TATACAACAG CAATTTCCAG AGGGACCTCC AGAGCAAATA 
CCTAGCCCTT TTCCAAAAAC TTTTGCTGAG GGCTTCTCCA TGAAAGCCTT TCCCACATCT 
GCCTCAGCTA GACAAGGCCG GTCCAGGATG CCTCCAGCTG CGAACAGCAG CCGCTACAGT 
ACACGCAGTC GGCTCTACTG CGCTCCCATG CAGGCCATAT CAGAAGGTGA AACCGAAAAC 
TCAGATGGAA GCCCACATGA TGACCGTTCC TCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

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NHSA cDNA ORF clone, Danio rerio(Zebrafish)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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