svila cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Gene Symbol	svila
Entrez Gene ID	30112
Full Name	supervillin a
Synonyms	sb:cb867,svil,wu:fd05a09
General protein information	Preferred Names supervillin a Names supervillin zmp:0000000648
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
XM_017358423.1	XP_017213912.1	supervillin isoform X1
XM_021480011.1	XP_021335686.1	supervillin isoform X1
NM_001035261.2	NP_001030338.2	supervillin
XM_021480012.1	XP_021335687.1	supervillin isoform X2
XM_021480016.1	XP_021335691.1	supervillin isoform X6
XM_021480014.1	XP_021335689.1	supervillin isoform X4
XM_021480013.1	XP_021335688.1	supervillin isoform X3
XM_021480015.1	XP_021335690.1	supervillin isoform X5

Pathways from BioSystems

Homologies

Bos taurus (cattle)	SVIL	NP_776615.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP007181	XP_565017.3
Danio rerio (zebrafish)	svilb	XP_003197996.2
Xenopus tropicalis (tropical clawed frog)	svil	NP_001090765.1
Pan troglodytes (chimpanzee)	SVIL	XP_001136112.1
Canis lupus familiaris (dog)	SVIL	XP_005617106.1
Mus musculus (house mouse)	Svil	NP_694793.1
Rattus norvegicus (Norway rat)	Svil	NP_001101886.2
Gallus gallus (chicken)	SVIL	XP_004939254.1
	SVIL	XP_001083894.2
Danio rerio (zebrafish)	svila	NP_001030338.2
Homo sapiens (human)	SVIL	NP_068506.2
Drosophila melanogaster (fruit fly)	CG33232	NP_995968.1

Gene Ontology
Bibliography

Related articles in PubMed

Evolution of complexity in the zebrafish synapse proteome.
Bay?s ?, Collins MO, Reig-Viader R, Gou G, Goulding D, Izquierdo A, Choudhary JS, Emes RD, Grant SG
Nature communications814613(2017 Mar)

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
Pasquier J, Cabau C, Nguyen T, Jouanno E, Severac D, Braasch I, Journot L, Pontarotti P, Klopp C, Postlethwait JH, Guiguen Y, Bobe J
BMC genomics17368(2016 05)

Supervillin Is a Component of the Hair Cell's Cuticular Plate and the Head Plates of Organ of Corti Supporting Cells.
Pollock LM, Gupta N, Chen X, Luna EJ, McDermott BM Jr
PloS one11(7)e0158349(2016)

RFX transcription factors are essential for hearing in mice.
Elkon R, Milon B, Morrison L, Shah M, Vijayakumar S, Racherla M, Leitch CC, Silipino L, Hadi S, Weiss-Gayet M, Barras E, Schmid CD, Ait-Lounis A, Barnes A, Song Y, Eisenman DJ, Eliyahu E, Frolenkov GI, Strome SE, Durand B, Zaghloul NA, Jones SM, Reith W, Hertzano R
Nature communications68549(2015 Oct)

Prioritizing causal disease genes using unbiased genomic features.
Deo RC, Musso G, Tasan M, Tang P, Poon A, Yuan C, Felix JF, Vasan RS, Beroukhim R, De Marco T, Kwok PY, MacRae CA, Roth FP
Genome biology15(12)534(2014 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following svila gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the svila cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODa148133	XM_021480015.1 Latest version!	Danio rerio supervillin a (svila), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
ODa17857	XM_021480016.1 Latest version!	Danio rerio supervillin a (svila), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
ODa32155	NM_001035261.2 Latest version!	Danio rerio supervillin a (svila), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
ODa148129	XM_021480011.1 Latest version!	Danio rerio supervillin a (svila), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1161.30 $1659.00
ODa148130	XM_021480012.1 Latest version!	Danio rerio supervillin a (svila), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1161.30 $1659.00
ODa148131	XM_021480013.1 Latest version!	Danio rerio supervillin a (svila), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1161.30 $1659.00
ODa148132	XM_021480014.1 Latest version!	Danio rerio supervillin a (svila), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1161.30 $1659.00
ODa54437	XM_017358423.1 Latest version!	Danio rerio supervillin a (svila), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1161.30 $1659.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

svila ( XM_021480015.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021335690.1 Danio rerio supervillin a (svila), transcript variant X5, mRNA.

Price & Availability↑

CloneID	ODa148133
Clone ID Related Accession (Same CDS sequence)	XM_021480015.1
Accession Version	XM_021480015.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5760bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	supervillin isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007123.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTATCGGA ACCCTTGGAT CTCCAACTAT ATCAACCATG TCAAGTTGTG CCAAGGCTTC 
ACCAGAGAAA CAGCCCCTAA AGTTGGCAAT ACCAAGCAAC GGAGAGCTCT TAGTTTTCAG 
AAAGAGTTCT CGTTTGAGGA GAAAGGTGAG GGAGCCAACA AAGAAGTAGA TGTTAGTCTG 
CTGGGGAATA CACCCAAGGT CTCAGAATTA AGGAAACGTT TTGAAAGTAT AAGCACAAGT 
CAGAGTTGGG ACATGAACAG GAAGGAGCGA ATTGCACGGC GCCTGGAAGG AATCGATAAT 
GATGTTCTCC AAAGTCTTCC AGGTTGTGGT GGTCTAGTTT CTAATCGACT GCTGGAGGAG 
GACACTCCAA GATATACCCG TGCAGCAGAC ACCTGTGACC CCTGTACAGT TTCTCTTCAG 
CACTATGGCA AAAGTGAACC AAGTTCACAA AGCACAGATG TTCCATCCAG AAGTTACGCA 
GTCCACCCGG AGTCTGTCTT CAGCACTGGC TCCTCTCATG TGGCAGAGCT GGACTCTAAA 
GCAGAAAGGA TTGCACGCTA CAAGGCTGAA CGCCGCCGTC AGTTAGCAGA GCGCTATGGC 
ATCTCTCTAG ATCAGGAGAT GGAAACAGAC TATACAACTC GCTTCTCCAG ACCCTCCAGA 
GAGCTTGATG GTTCAGACAG ACACCACAGG TCAAAATCAG AAGGGAGAGA TGGAGTCGAG 
CAAAATGCTT ACACCAGCAG CCATCATGTG GATAAGAGCA GCTCAGACTA TCACTCCAGT 
CCAGAGTACA GCCGCGAGCG CACAGACTCC ATCTCAGAAC GTGAAAGAAT GATGAATCTG 
GAGAACCAGA GGAGGGCACA GGAGCGTGAC CGATTACATG GAGGTGGAGG GGTTGCACCT 
GACCCATCTG CGTATATGGA TGTGTCTGGA TCTGCAAGGG TACCAGGAAG GGAGCCAGGG 
ATGATGGGAG TTCCCAGCTC ACCCAATTCA GGCCGAAGGG GCTTGATGCT CTCTCCCAAA 
CAGGGAGCGT CTCCTGGTGA TCTGTTTATT GAGCAGCAGG CTCACAGCAT CCTCCAAAGA 
CACGGGTCTC ATCTAAGCTC TGAACCATCA AGCCCTGAGG TGGATGAAGA GAAACTAGAT 
GAGAGAGCAA AGCTGAGCGT AGCTGCCAAG CGTTCCCTCT TCAGGGAGCT TGAAAAGACT 
TCAGATGGTT CCGTTTTAAA GACATGGAGT CGTAATCCTG CTGTTGAGCG GAGATTGAGG 
AGAGGTCAAG ATCGATCCCG CACTCAGCCA GTCACCACAG AGGAAGTAGT TATTGCTGCT 
ACTGCCCCTG GCCATTTGTC ACAGTCCGTG ACTTTACAAA CCTCTATAAC GCGCGTTCCT 
AGTCCCACTC TAGTCAGCAC CTCTGTGACC AAACACAGCT TGCAGGCTTC GTCTCAGCAA 
AGCTCCATGG TGAGAGAGCA GGCCAGAGAG GTTCGTCTGG CTCAGGAGGC AACGGAGACA 
GATCGAGCTC GCCACGCTTC TCCGGGGGAA GGCGGACACG AGGAGCCAGA CCTTTCCACA 
CTCACTCTAG CAGAAAAGAT GGCACTTTTT AACCGTCTTG CCCAGCCGTC CAGCCACGGC 
TCTAGGGCCA GAGGAGACAC GCGTGCACGG AGGAGTAACG CACGCTATCA GACTCAACCA 
ATCACACTAG GAGAAGTCGA ACAGGATGGT TCTGGTTCTG AGATGTTCGA TGAGGAATCT 
GAGTTCTCAA TGTGTCTCAG TGATCTGGAA CATTTTCAGC CTGCAGGTGG AGCTCGTTTT 
GCACCCAGCT CCAACTCAGC CATGAGATCC TCCACTGTTT CCACTGAACA TGCGGGCGAC 
ATCCATCTGA CCATGCCAAA TGTATCTGGG CCTGCATATT ATGAGCAGAC CGTTTCTCCA 
CCGCATCACC CTCAGCCTGC CCATGAGATC CAGACACATC AGAGTCTGGC TGAAGGGGAA 
AGGCTGCTGT TGAGCACTGG GAAGAGGAAG CTTCCCTCCC CAGACAGCGT GAAACGGACA 
CCGCATGCTC AGCCGGCCAG AGACTGGGAC AGTAAGGCTG TGGCGGTGAC CTCTCAAGAC 
CTTTCACAAA CAGATGGAGA GCACACAACA GATCTTCCTG TACACACTGC CGCTGCTGTA 
TCTGCATTAC AGAGCCATTC TCTCCATGAG GACCACACCA GAGAGGAAGA GCAGGAGAGC 
GACTTCAAAA CAGAAGACGG AGACGAGCTC TCGGACGTCA TGTCTGCCAG ACAGATGTCC 
ATCAAAGAGA GGCTGGCTCT GCTGAAGAAG AGTGGAGAGG AAGACTGGAG GAACAGGATA 
AATAAAAAAC AGGATGTGGT GAAGGTTGCT GTGTCTGAAA GACATGCCCA GCTATGGGAG 
GTGGAGCAGA GCTTCAAGAA GAAGGATGAT GGGATGATGA TAGATGACTT TGCTGTGTCT 
GATCAACTCT GGGATCCTGT CTTTTCCTCC TCCCTTTTGT GTGCCTCTGA ACCACTGGAT 
CAAATAGCAA ACCTTCCCAA GGCTACCCTC CTGCCCGTGC AGGTGGATGA GCGTCACCCC 
TGGAGACGGA AGAAGATGGG AAATGAAGTT GTGGAAAGCC AACAGCTTGA GCGAGTAGAG 
ATGTCTATTC AGGAACGCAA GCAGCTCATC ACTTCACAAG AGGAGGCCTG GAAAGCCAAA 
GGTTACGGAG CAGCCAACGA CTCCACTCAG TTTACAGTGG CTGGACGTAT GGTCAAAAAA 
GGCCTGGCAG CCCCTTCTGT TCTCACAAAT CCCGTGTTGT CTCCTCTGTC TTCTAAAAAC 
AAGAATACAT CCCATACCAT CACAAAACCA CATGAAGAGA TTGAAGTCAA ACAGAACATG 
AAGCTGGAGT CAGACATGAA GTTAGAAAAG TTGGAAACAT TCCTTGGTCG TTTGAATAGT 
AAAGTTCCAG GACTTCAGGA GGCCACAATT GCTGTCACGG AGAAGTCTGT AAAAGAGGTC 
ATGACTCTGG ACGATGAGGT CTTTGATAAG TTTTATCGAC ATGCAGAGGA TATGGCAAAG 
TTCACCAGCA GAGTGGAAAT TAGTGATGAT TTTGATGACA TCTTTGGAGT ACAGCTGCCC 
AGGTTGACTT CGGAAATGGT GCAGCACAAG CGGGCAGTTC GGCCTACCAG AAATGTTCAG 
TCATCTAAAA ACCCGCTGAA AATGCTGGCT GTTCGGGAGG ACATCAGACA TGAGTACACG 
GAACAAAGAC TGAACATCGG CCTTCTGGAG AGCAAGAGGA TGAAACAGGA AAAGATGAAT 
AAGAACAGCG GTTTCTCTGA GGTTGCTCTC GCCGGATTGG CCAGTAAGGA GAACTTCAGC 
AGTGTGAGTC TGCGAAGTGT GAATGCCTCT GAACAAAGCT CCAACAACAG TGCCATGCCC 
TATAAAAAAC TCATGCTCAT TCAGGTCAAA GGTCGCAGGC ACGTTCAGAC CAGACTGGTT 
GAGCCCAGAG CTTCCTCCTT GAACAGTGGC GACTGCTTCC TCCTGATCAC ACCACATCAT 
TGCTTCATCT GGATTGGAGA ATTCGCTAAT GTTATTGAGA AGGCCAAAGC TGCAGAGTTG 
GCCACGTTCG TTCAAACCAA GCATGACCTG GGATGCAGAG CCTCGTATGT TCAGACAATT 
GAGGAGGGTG CAAACACTCA CACACACGCT GCCAAAGACT TTTGGAAGAT TCTCGGTGGC 
CAAACCAGTT ATCAGTCCGC TGGCACTCCT GAACAAGATG AGTTTTATGA GAGTGCAGTC 
GTAGAAACCA ACTGCATATA CAGACTCATG GAGGATAAAC TTGTTCCCCA TGATGACTAC 
TGGGGTCGAG TCCCTCGCTG CTCCATGCTG AACCCTAAAG AGGTGCTGGT ATTTGATTTT 
GGTAGTGAGG TCTATGTATG GCATGGCAAG GAGGTGACGT TGGCACAGAG AAAAGTGGCA 
TTTCAGCTGG CGAAGCACTT GTGGAATGGC ACCTTTGACT ACACCAACTG TGACATTAAT 
CCTCTGGACC CTGGAGAGTG CAATGCTCTC ATACCCAGGA AAGGCCAGGG ACGACCAGAC 
TGGGCTGTGT TTGGCAGGTT AACTCAGCAC AATGAAACTA CTCTTTTTAA AGAGAAGTTT 
TTGGACTGGA GTGATTCCAA GAATTCTAAG AAAAATGGTG ACTTGCATGT TGAAAACAAG 
GAGCTTCGGA GTGGTGAATG TCGGCCCTAT GATGCGTCTC TGATGCTATC TGCATTACGG 
GCGCCAGTCA GGACCGTTCT GGATGGGGTG AACATTGGAC GAGGATATGG ATTAGTGGAA 
GGTGAAGAGG GACGTAATTT TGAGATCAGC ACATTGTCTG TGGATGTCTG GCATATTTTA 
GAGTTTGACT ACAGTCGACT ACCCAAACAG AGCATTGGTC AGTTTCATGA AGGTGACACA 
TACGTGGTCA AATGGAAGTA CATGATCAGC AGAGCAGTTG GCAAGAGACT GCATTCAGAA 
AGAATCATCG GTCCAGGGAA GGAGAAATGT TGTTATTTCT TCTGGCAGGG TCGTAACTCT 
ACGGTGAATG AAAAGGGAAC GTCAGCACTG ATGACTGTTG AGCTGGATGA GGAAAGAGGA 
GCTCAGGTCC AGGTACAGCA AGGAAAGGAG CCTCCTTGTT TCCTACAGTG CTTCAATGGA 
GGAATGATTG TTCATGCTGG AAAAAGAGAA GAGGAGGAAG ACAATACACA AAACGACTGG 
CGATTGTACT GTGTGAGAGG AGAGAAACCC ATAGAGGGCC ATCTGCTGGA GGCAGTGTGT 
CACTGCAGCA GCTTGCGATC CCGGACCTCT ATGATCCTCC TGAACATCCC CAAAGCCAGC 
CTATACCTGT GGCATGGCTG CAAGGCACAA GTGCACACAC GTGACGTGGG CCGTACCACT 
GCAAACAAAA TCAAAGAGCA ATGTCCTCTG GAGGCAGGGC TTCACAGCAG CAGTAAAGTG 
TCCATCCAGG AGTGTGATGA GGGAGCTGAA CCACAGGGCT TCTGGGAGGC TTTGGGCAAA 
AGAGATCGAA AAGCGTACGA CTGCATGCTG CAGGATCCAG GAAAGTTCAA CTTCACTCCT 
CGGCTTTTCC AGTTAAGCAG TGCCTCGGGA GAGTTTGCAG CAGTGGAGTT TGTATATCCA 
TCCAGAGAAC CAAATTTGGT TAACTCGATG CCTTTCCTCC AGGAGGATTT GTATACAGCT 
ACTCAGCCAG CCCTGTTTTT GGTGGATAAT CATCATGAAG TTTACCTGTG GCAGGGCTGG 
TGGCCTCAGG ACAGTGAAAG CACAGGCTCA GCTCGCATAC GGTGGGACTC TGACAGGAAG 
TGTGCAATGG AAACTGTGCT CCAGTATAGC AAAGAAAAAA ATGAGAAAAA GACACCAAAA 
TCCTACCTGA TCCATGCAGG ACTGGAGCCT CTTACATTCA CCAACATGTT TCCCAGCTGG 
GAACACAGAG AGGACATCGC TGAAATCACA GAGAAGGAGG CCGAAGTATG TCATCAGATC 
ATTCTTGTTG AAGATGTCTT GAGCCGACTC TGCAAGACCA CTTACCCACT GGCTGAACTA 
AAAGCACGTC CCTTACCGGA AGGTGTCGAC CCTCTACGTC TTGAAACCTA CTTATCAGAT 
GAGGACTTTG AGGGTGTAGG GGCTTCTGGG AAGAAAGCTT TGGAGATGAC GAGAAGTGAA 
TATGAGGCAC TGCCTGGATG GAAGCAAGTG AATGTGAAGA AAGCCAAAGG ATTGTTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021335690.1
CDS	340..6099
Translation	MYRNPWISNYINHVKLCQGFTRETAPKVGNTKQRRALSFQKEFSFEEKGEGANKEVDVSLLGNTPKVSELRKRFESISTSQSWDMNRKERIARRLEGIDNDVLQSLPGCGGLVSNRLLEEDTPRYTRAADTCDPCTVSLQHYGKSEPSSQSTDVPSRSYAVHPESVFSTGSSHVAELDSKAERIARYKAERRRQLAERYGISLDQEMETDYTTRFSRPSRELDGSDRHHRSKSEGRDGVEQNAYTSSHHVDKSSSDYHSSPEYSRERTDSISERERMMNLENQRRAQERDRLHGGGGVAPDPSAYMDVSGSARVPGREPGMMGVPSSPNSGRRGLMLSPKQGASPGDLFIEQQAHSILQRHGSHLSSEPSSPEVDEEKLDERAKLSVAAKRSLFRELEKTSDGSVLKTWSRNPAVERRLRRGQDRSRTQPVTTEEVVIAATAPGHLSQSVTLQTSITRVPSPTLVSTSVTKHSLQASSQQSSMVREQAREVRLAQEATETDRARHASPGEGGHEEPDLSTLTLAEKMALFNRLAQPSSHGSRARGDTRARRSNARYQTQPITLGEVEQDGSGSEMFDEESEFSMCLSDLEHFQPAGGARFAPSSNSAMRSSTVSTEHAGDIHLTMPNVSGPAYYEQTVSPPHHPQPAHEIQTHQSLAEGERLLLSTGKRKLPSPDSVKRTPHAQPARDWDSKAVAVTSQDLSQTDGEHTTDLPVHTAAAVSALQSHSLHEDHTREEEQESDFKTEDGDELSDVMSARQMSIKERLALLKKSGEEDWRNRINKKQDVVKVAVSERHAQLWEVEQSFKKKDDGMMIDDFAVSDQLWDPVFSSSLLCASEPLDQIANLPKATLLPVQVDERHPWRRKKMGNEVVESQQLERVEMSIQERKQLITSQEEAWKAKGYGAANDSTQFTVAGRMVKKGLAAPSVLTNPVLSPLSSKNKNTSHTITKPHEEIEVKQNMKLESDMKLEKLETFLGRLNSKVPGLQEATIAVTEKSVKEVMTLDDEVFDKFYRHAEDMAKFTSRVEISDDFDDIFGVQLPRLTSEMVQHKRAVRPTRNVQSSKNPLKMLAVREDIRHEYTEQRLNIGLLESKRMKQEKMNKNSGFSEVALAGLASKENFSSVSLRSVNASEQSSNNSAMPYKKLMLIQVKGRRHVQTRLVEPRASSLNSGDCFLLITPHHCFIWIGEFANVIEKAKAAELATFVQTKHDLGCRASYVQTIEEGANTHTHAAKDFWKILGGQTSYQSAGTPEQDEFYESAVVETNCIYRLMEDKLVPHDDYWGRVPRCSMLNPKEVLVFDFGSEVYVWHGKEVTLAQRKVAFQLAKHLWNGTFDYTNCDINPLDPGECNALIPRKGQGRPDWAVFGRLTQHNETTLFKEKFLDWSDSKNSKKNGDLHVENKELRSGECRPYDASLMLSALRAPVRTVLDGVNIGRGYGLVEGEEGRNFEISTLSVDVWHILEFDYSRLPKQSIGQFHEGDTYVVKWKYMISRAVGKRLHSERIIGPGKEKCCYFFWQGRNSTVNEKGTSALMTVELDEERGAQVQVQQGKEPPCFLQCFNGGMIVHAGKREEEEDNTQNDWRLYCVRGEKPIEGHLLEAVCHCSSLRSRTSMILLNIPKASLYLWHGCKAQVHTRDVGRTTANKIKEQCPLEAGLHSSSKVSIQECDEGAEPQGFWEALGKRDRKAYDCMLQDPGKFNFTPRLFQLSSASGEFAAVEFVYPSREPNLVNSMPFLQEDLYTATQPALFLVDNHHEVYLWQGWWPQDSESTGSARIRWDSDRKCAMETVLQYSKEKNEKKTPKSYLIHAGLEPLTFTNMFPSWEHREDIAEITEKEAEVCHQIILVEDVLSRLCKTTYPLAELKARPLPEGVDPLRLETYLSDEDFEGVGASGKKALEMTRSEYEALPGWKQVNVKKAKGLF

Target ORF information:

RefSeq Version	XM_021480015.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio supervillin a (svila), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021480015.1

ORF Insert Sequence:

ATGTATCGGA ACCCTTGGAT CTCCAACTAT ATCAACCATG TCAAGTTGTG CCAAGGCTTC 
ACCAGAGAAA CAGCCCCTAA AGTTGGCAAT ACCAAGCAAC GGAGAGCTCT TAGTTTTCAG 
AAAGAGTTCT CGTTTGAGGA GAAAGGTGAG GGAGCCAACA AAGAAGTAGA TGTTAGTCTG 
CTGGGGAATA CACCCAAGGT CTCAGAATTA AGGAAACGTT TTGAAAGTAT AAGCACAAGT 
CAGAGTTGGG ACATGAACAG GAAGGAGCGA ATTGCACGGC GCCTGGAAGG AATCGATAAT 
GATGTTCTCC AAAGTCTTCC AGGTTGTGGT GGTCTAGTTT CTAATCGACT GCTGGAGGAG 
GACACTCCAA GATATACCCG TGCAGCAGAC ACCTGTGACC CCTGTACAGT TTCTCTTCAG 
CACTATGGCA AAAGTGAACC AAGTTCACAA AGCACAGATG TTCCATCCAG AAGTTACGCA 
GTCCACCCGG AGTCTGTCTT CAGCACTGGC TCCTCTCATG TGGCAGAGCT GGACTCTAAA 
GCAGAAAGGA TTGCACGCTA CAAGGCTGAA CGCCGCCGTC AGTTAGCAGA GCGCTATGGC 
ATCTCTCTAG ATCAGGAGAT GGAAACAGAC TATACAACTC GCTTCTCCAG ACCCTCCAGA 
GAGCTTGATG GTTCAGACAG ACACCACAGG TCAAAATCAG AAGGGAGAGA TGGAGTCGAG 
CAAAATGCTT ACACCAGCAG CCATCATGTG GATAAGAGCA GCTCAGACTA TCACTCCAGT 
CCAGAGTACA GCCGCGAGCG CACAGACTCC ATCTCAGAAC GTGAAAGAAT GATGAATCTG 
GAGAACCAGA GGAGGGCACA GGAGCGTGAC CGATTACATG GAGGTGGAGG GGTTGCACCT 
GACCCATCTG CGTATATGGA TGTGTCTGGA TCTGCAAGGG TACCAGGAAG GGAGCCAGGG 
ATGATGGGAG TTCCCAGCTC ACCCAATTCA GGCCGAAGGG GCTTGATGCT CTCTCCCAAA 
CAGGGAGCGT CTCCTGGTGA TCTGTTTATT GAGCAGCAGG CTCACAGCAT CCTCCAAAGA 
CACGGGTCTC ATCTAAGCTC TGAACCATCA AGCCCTGAGG TGGATGAAGA GAAACTAGAT 
GAGAGAGCAA AGCTGAGCGT AGCTGCCAAG CGTTCCCTCT TCAGGGAGCT TGAAAAGACT 
TCAGATGGTT CCGTTTTAAA GACATGGAGT CGTAATCCTG CTGTTGAGCG GAGATTGAGG 
AGAGGTCAAG ATCGATCCCG CACTCAGCCA GTCACCACAG AGGAAGTAGT TATTGCTGCT 
ACTGCCCCTG GCCATTTGTC ACAGTCCGTG ACTTTACAAA CCTCTATAAC GCGCGTTCCT 
AGTCCCACTC TAGTCAGCAC CTCTGTGACC AAACACAGCT TGCAGGCTTC GTCTCAGCAA 
AGCTCCATGG TGAGAGAGCA GGCCAGAGAG GTTCGTCTGG CTCAGGAGGC AACGGAGACA 
GATCGAGCTC GCCACGCTTC TCCGGGGGAA GGCGGACACG AGGAGCCAGA CCTTTCCACA 
CTCACTCTAG CAGAAAAGAT GGCACTTTTT AACCGTCTTG CCCAGCCGTC CAGCCACGGC 
TCTAGGGCCA GAGGAGACAC GCGTGCACGG AGGAGTAACG CACGCTATCA GACTCAACCA 
ATCACACTAG GAGAAGTCGA ACAGGATGGT TCTGGTTCTG AGATGTTCGA TGAGGAATCT 
GAGTTCTCAA TGTGTCTCAG TGATCTGGAA CATTTTCAGC CTGCAGGTGG AGCTCGTTTT 
GCACCCAGCT CCAACTCAGC CATGAGATCC TCCACTGTTT CCACTGAACA TGCGGGCGAC 
ATCCATCTGA CCATGCCAAA TGTATCTGGG CCTGCATATT ATGAGCAGAC CGTTTCTCCA 
CCGCATCACC CTCAGCCTGC CCATGAGATC CAGACACATC AGAGTCTGGC TGAAGGGGAA 
AGGCTGCTGT TGAGCACTGG GAAGAGGAAG CTTCCCTCCC CAGACAGCGT GAAACGGACA 
CCGCATGCTC AGCCGGCCAG AGACTGGGAC AGTAAGGCTG TGGCGGTGAC CTCTCAAGAC 
CTTTCACAAA CAGATGGAGA GCACACAACA GATCTTCCTG TACACACTGC CGCTGCTGTA 
TCTGCATTAC AGAGCCATTC TCTCCATGAG GACCACACCA GAGAGGAAGA GCAGGAGAGC 
GACTTCAAAA CAGAAGACGG AGACGAGCTC TCGGACGTCA TGTCTGCCAG ACAGATGTCC 
ATCAAAGAGA GGCTGGCTCT GCTGAAGAAG AGTGGAGAGG AAGACTGGAG GAACAGGATA 
AATAAAAAAC AGGATGTGGT GAAGGTTGCT GTGTCTGAAA GACATGCCCA GCTATGGGAG 
GTGGAGCAGA GCTTCAAGAA GAAGGATGAT GGGATGATGA TAGATGACTT TGCTGTGTCT 
GATCAACTCT GGGATCCTGT CTTTTCCTCC TCCCTTTTGT GTGCCTCTGA ACCACTGGAT 
CAAATAGCAA ACCTTCCCAA GGCTACCCTC CTGCCCGTGC AGGTGGATGA GCGTCACCCC 
TGGAGACGGA AGAAGATGGG AAATGAAGTT GTGGAAAGCC AACAGCTTGA GCGAGTAGAG 
ATGTCTATTC AGGAACGCAA GCAGCTCATC ACTTCACAAG AGGAGGCCTG GAAAGCCAAA 
GGTTACGGAG CAGCCAACGA CTCCACTCAG TTTACAGTGG CTGGACGTAT GGTCAAAAAA 
GGCCTGGCAG CCCCTTCTGT TCTCACAAAT CCCGTGTTGT CTCCTCTGTC TTCTAAAAAC 
AAGAATACAT CCCATACCAT CACAAAACCA CATGAAGAGA TTGAAGTCAA ACAGAACATG 
AAGCTGGAGT CAGACATGAA GTTAGAAAAG TTGGAAACAT TCCTTGGTCG TTTGAATAGT 
AAAGTTCCAG GACTTCAGGA GGCCACAATT GCTGTCACGG AGAAGTCTGT AAAAGAGGTC 
ATGACTCTGG ACGATGAGGT CTTTGATAAG TTTTATCGAC ATGCAGAGGA TATGGCAAAG 
TTCACCAGCA GAGTGGAAAT TAGTGATGAT TTTGATGACA TCTTTGGAGT ACAGCTGCCC 
AGGTTGACTT CGGAAATGGT GCAGCACAAG CGGGCAGTTC GGCCTACCAG AAATGTTCAG 
TCATCTAAAA ACCCGCTGAA AATGCTGGCT GTTCGGGAGG ACATCAGACA TGAGTACACG 
GAACAAAGAC TGAACATCGG CCTTCTGGAG AGCAAGAGGA TGAAACAGGA AAAGATGAAT 
AAGAACAGCG GTTTCTCTGA GGTTGCTCTC GCCGGATTGG CCAGTAAGGA GAACTTCAGC 
AGTGTGAGTC TGCGAAGTGT GAATGCCTCT GAACAAAGCT CCAACAACAG TGCCATGCCC 
TATAAAAAAC TCATGCTCAT TCAGGTCAAA GGTCGCAGGC ACGTTCAGAC CAGACTGGTT 
GAGCCCAGAG CTTCCTCCTT GAACAGTGGC GACTGCTTCC TCCTGATCAC ACCACATCAT 
TGCTTCATCT GGATTGGAGA ATTCGCTAAT GTTATTGAGA AGGCCAAAGC TGCAGAGTTG 
GCCACGTTCG TTCAAACCAA GCATGACCTG GGATGCAGAG CCTCGTATGT TCAGACAATT 
GAGGAGGGTG CAAACACTCA CACACACGCT GCCAAAGACT TTTGGAAGAT TCTCGGTGGC 
CAAACCAGTT ATCAGTCCGC TGGCACTCCT GAACAAGATG AGTTTTATGA GAGTGCAGTC 
GTAGAAACCA ACTGCATATA CAGACTCATG GAGGATAAAC TTGTTCCCCA TGATGACTAC 
TGGGGTCGAG TCCCTCGCTG CTCCATGCTG AACCCTAAAG AGGTGCTGGT ATTTGATTTT 
GGTAGTGAGG TCTATGTATG GCATGGCAAG GAGGTGACGT TGGCACAGAG AAAAGTGGCA 
TTTCAGCTGG CGAAGCACTT GTGGAATGGC ACCTTTGACT ACACCAACTG TGACATTAAT 
CCTCTGGACC CTGGAGAGTG CAATGCTCTC ATACCCAGGA AAGGCCAGGG ACGACCAGAC 
TGGGCTGTGT TTGGCAGGTT AACTCAGCAC AATGAAACTA CTCTTTTTAA AGAGAAGTTT 
TTGGACTGGA GTGATTCCAA GAATTCTAAG AAAAATGGTG ACTTGCATGT TGAAAACAAG 
GAGCTTCGGA GTGGTGAATG TCGGCCCTAT GATGCGTCTC TGATGCTATC TGCATTACGG 
GCGCCAGTCA GGACCGTTCT GGATGGGGTG AACATTGGAC GAGGATATGG ATTAGTGGAA 
GGTGAAGAGG GACGTAATTT TGAGATCAGC ACATTGTCTG TGGATGTCTG GCATATTTTA 
GAGTTTGACT ACAGTCGACT ACCCAAACAG AGCATTGGTC AGTTTCATGA AGGTGACACA 
TACGTGGTCA AATGGAAGTA CATGATCAGC AGAGCAGTTG GCAAGAGACT GCATTCAGAA 
AGAATCATCG GTCCAGGGAA GGAGAAATGT TGTTATTTCT TCTGGCAGGG TCGTAACTCT 
ACGGTGAATG AAAAGGGAAC GTCAGCACTG ATGACTGTTG AGCTGGATGA GGAAAGAGGA 
GCTCAGGTCC AGGTACAGCA AGGAAAGGAG CCTCCTTGTT TCCTACAGTG CTTCAATGGA 
GGAATGATTG TTCATGCTGG AAAAAGAGAA GAGGAGGAAG ACAATACACA AAACGACTGG 
CGATTGTACT GTGTGAGAGG AGAGAAACCC ATAGAGGGCC ATCTGCTGGA GGCAGTGTGT 
CACTGCAGCA GCTTGCGATC CCGGACCTCT ATGATCCTCC TGAACATCCC CAAAGCCAGC 
CTATACCTGT GGCATGGCTG CAAGGCACAA GTGCACACAC GTGACGTGGG CCGTACCACT 
GCAAACAAAA TCAAAGAGCA ATGTCCTCTG GAGGCAGGGC TTCACAGCAG CAGTAAAGTG 
TCCATCCAGG AGTGTGATGA GGGAGCTGAA CCACAGGGCT TCTGGGAGGC TTTGGGCAAA 
AGAGATCGAA AAGCGTACGA CTGCATGCTG CAGGATCCAG GAAAGTTCAA CTTCACTCCT 
CGGCTTTTCC AGTTAAGCAG TGCCTCGGGA GAGTTTGCAG CAGTGGAGTT TGTATATCCA 
TCCAGAGAAC CAAATTTGGT TAACTCGATG CCTTTCCTCC AGGAGGATTT GTATACAGCT 
ACTCAGCCAG CCCTGTTTTT GGTGGATAAT CATCATGAAG TTTACCTGTG GCAGGGCTGG 
TGGCCTCAGG ACAGTGAAAG CACAGGCTCA GCTCGCATAC GGTGGGACTC TGACAGGAAG 
TGTGCAATGG AAACTGTGCT CCAGTATAGC AAAGAAAAAA ATGAGAAAAA GACACCAAAA 
TCCTACCTGA TCCATGCAGG ACTGGAGCCT CTTACATTCA CCAACATGTT TCCCAGCTGG 
GAACACAGAG AGGACATCGC TGAAATCACA GAGAAGGAGG CCGAAGTATG TCATCAGATC 
ATTCTTGTTG AAGATGTCTT GAGCCGACTC TGCAAGACCA CTTACCCACT GGCTGAACTA 
AAAGCACGTC CCTTACCGGA AGGTGTCGAC CCTCTACGTC TTGAAACCTA CTTATCAGAT 
GAGGACTTTG AGGGTGTAGG GGCTTCTGGG AAGAAAGCTT TGGAGATGAC GAGAAGTGAA 
TATGAGGCAC TGCCTGGATG GAAGCAAGTG AATGTGAAGA AAGCCAAAGG ATTGTTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

svila ( XM_021480016.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021335691.1 Danio rerio supervillin a (svila), transcript variant X6, mRNA.

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CloneID	ODa17857
Clone ID Related Accession (Same CDS sequence)	XM_021480016.1
Accession Version	XM_021480016.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5544bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	supervillin isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007123.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTATCGGA ACCCTTGGAT CTCCAACTAT ATCAACCATG TCAAGTTGTG CCAAGGCTTC 
ACCAGAGAAA CAGCCCCTAA AGTTGGCAAT ACCAAGCAAC GGAGAGCTCT TAGTTTTCAG 
AAAGAGTTCT CGTTTGAGGA GAAAGGTGAG GGAGCCAACA AAGAAGTAGA TGTTAGTCTG 
CTGGGGAATA CACCCAAGGT CTCAGAATTA AGGAAACGTT TTGAAAGTAT AAGCACAAGT 
CAGAGTTGGG ACATGAACAG GAAGGAGCGA ATTGCACGGC GCCTGGAAGG AATCGATAAT 
GATGTTCTCC AAAGTCTTCC AGGTTGTGGT GGTCTAGTTT CTAATCGACT GCTGGAGGAG 
GACACTCCAA GATATACCCG TGCAGCAGAC ACCTGTGACC CCTGTACAGT TTCTCTTCAG 
CACTATGGCA AAAGTGAACC AAGTTCACAA AGCACAGATG TTCCATCCAG AAGTTACGCA 
GTCCACCCGG AGTCTGTCTT CAGCACTGGC TCCTCTCATG TGGCAGAGCT GGACTCTAAA 
GCAGAAAGGA TTGCACGCTA CAAGGCTGAA CGCCGCCGTC AGTTAGCAGA GCGCTATGGC 
ATCTCTCTAG ATCAGGAGAT GGAAACAGAC TATACAACTC GCTTCTCCAG ACCCTCCAGA 
GAGCTTGATG GTTCAGACAG ACACCACAGG TCAAAATCAG AAGGGAGAGA TGGAGTCGAG 
CAAAATGCTT ACACCAGCAG CCATCATGTG GATAAGAGCA GCTCAGACTA TCACTCCAGT 
CCAGAGTACA GCCGCGAGCG CACAGACTCC ATCTCAGAAC GTGAAAGAAT GATGAATCTG 
GAGAACCAGA GGAGGGCACA GGAGCGTGAC CGATTACATG GAGGTGGAGG GGTTGCACCT 
GACCCATCTG CGTATATGGA TGTGTCTGGA TCTGCAAGGG TACCAGGAAG GGAGCCAGGG 
ATGATGGGAG TTCCCAGCTC ACCCAATTCA GGCCGAAGGG GCTTGATGCT CTCTCCCAAA 
CAGGGAGCGT CTCCTGGTGA TCTGTTTATT GAGCAGCAGG CTCACAGCAT CCTCCAAAGA 
CACGGGTCTC ATCTAAGCTC TGAACCATCA AGCCCTGAGG TGGATGAAGA GAAACTAGAT 
GAGAGAGCAA AGCTGAGCGT AGCTGCCAAG CGTTCCCTCT TCAGGGAGCT TGAAAAGACT 
TCAGATGGTT CCGTTTTAAA GACATGGAGT CGTAATCCTG CTGTTGAGCG GAGATTGAGG 
AGAGGTCAAG ATCGATCCCG CACTCAGCCA GTCACCACAG AGGAAGTAGT TATTGCTGCT 
ACCTTGCAGG CTTCGTCTCA GCAAAGCTCC ATGGTGAGAG AGCAGGCCAG AGAGGTTCGT 
CTGGCTCAGG AGGCAACGGA GACAGATCGA GCTCGCCACG CTTCTCCGGG GGAAGGCGGA 
CACGAGGAGC CAGACCTTTC CACACTCACT CTAGCAGAAA AGATGGCACT TTTTAACCGT 
CTTGCCCAGC CGTCCAGCCA CGGCTCTAGG GCCAGAGGAG ACACGCGTGC ACGGAGGAGT 
AACGCACGCT ATCAGACTCA ACCAATCACA CTAGGAGAAG TCGAACAGTT TCAGCCTGCA 
GGTGGAGCTC GTTTTGCACC CAGCTCCAAC TCAGCCATGA GATCCTCCAC TGTTTCCACT 
GAACATGCGG GCGACATCCA TCTGACCATG CCAAATGTAT CTGGGCCTGC ATATTATGAG 
CAGACCGTTT CTCCACCGCA TCACCCTCAG CCTGCCCATG AGATCCAGAC ACATCAGAGT 
CTGGCTGAAG GGGAAAGGCT GCTGTTGAGC ACTGGGAAGA GGAAGCTTCC CTCCCCAGAC 
AGCGTGAAAC GGACACCGCA TGCTCAGCCG GCCAGAGACT GGGACAGTAA GGCTGTGGCG 
GTGACCTCTC AAGACCTTTC ACAAACAGAT GGAGAGCACA CAACAGATCT TCCTGTACAC 
ACTGCCGCTG CTGTATCTGC ATTACAGAGC CATTCTCTCC ATGAGGACCA CACCAGAGAG 
GAAGAGCAGG AGAGCGACTT CAAAACAGAA GACGGAGACG AGCTCTCGGA CGTCATGTCT 
GCCAGACAGA TGTCCATCAA AGAGAGGCTG GCTCTGCTGA AGAAGAGTGG AGAGGAAGAC 
TGGAGGAACA GGATAAATAA AAAACAGGAT GTGGTGAAGG TTGCTGTGTC TGAAAGACAT 
GCCCAGCTAT GGGAGGTGGA GCAGAGCTTC AAGAAGAAGG ATGATGGGAT GATGATAGAT 
GACTTTGCTG TGTCTGATCA ACTCTGGGAT CCTGTCTTTT CCTCCTCCCT TTTGTGTGCC 
TCTGAACCAC TGGATCAAAT AGCAAACCTT CCCAAGAAGA TGGGAAATGA AGTTGTGGAA 
AGCCAACAGC TTGAGCGAGT AGAGATGTCT ATTCAGGAAC GCAAGCAGCT CATCACTTCA 
CAAGAGGAGG CCTGGAAAGC CAAAGGTTAC GGAGCAGCCA ACGACTCCAC TCAGTTTACA 
GTGGCTGGAC GTATGGTCAA AAAAGGCCTG GCAGCCCCTT CTGTTCTCAC AAATCCCGTG 
TTGTCTCCTC TGTCTTCTAA AAACAAGAAT ACATCCCATA CCATCACAAA ACCACATGAA 
GAGATTGAAG TCAAACAGAA CATGAAGCTG GAGTCAGACA TGAAGTTAGA AAAGTTGGAA 
ACATTCCTTG GTCGTTTGAA TAGTAAAGTT CCAGGACTTC AGGAGGCCAC AATTGCTGTC 
ACGGAGAAGT CTGTAAAAGA GGTCATGACT CTGGACGATG AGGTCTTTGA TAAGTTTTAT 
CGACATGCAG AGGATATGGC AAAGTTCACC AGCAGAGTGG AAATTAGTGA TGATTTTGAT 
GACATCTTTG GAGTACAGCT GCCCAGGTTG ACTTCGGAAA TGGTGCAGCA CAAGCGGGCA 
GTTCGGCCTA CCAGAAATGT TCAGTCATCT AAAAACCCGC TGAAAATGCT GGCTGTTCGG 
GAGGACATCA GACATGAGTA CACGGAACAA AGACTGAACA TCGGCCTTCT GGAGAGCAAG 
AGGATGAAAC AGGAAAAGAT GAATAAGAAC AGCGGTTTCT CTGAGGTTGC TCTCGCCGGA 
TTGGCCAGTA AGGAGAACTT CAGCAGTGTG AGTCTGCGAA GTGTGAATGC CTCTGAACAA 
AGCTCCAACA ACAGTGCCAT GCCCTATAAA AAACTCATGC TCATTCAGGT CAAAGGTCGC 
AGGCACGTTC AGACCAGACT GGTTGAGCCC AGAGCTTCCT CCTTGAACAG TGGCGACTGC 
TTCCTCCTGA TCACACCACA TCATTGCTTC ATCTGGATTG GAGAATTCGC TAATGTTATT 
GAGAAGGCCA AAGCTGCAGA GTTGGCCACG TTCGTTCAAA CCAAGCATGA CCTGGGATGC 
AGAGCCTCGT ATGTTCAGAC AATTGAGGAG GGTGCAAACA CTCACACACA CGCTGCCAAA 
GACTTTTGGA AGATTCTCGG TGGCCAAACC AGTTATCAGT CCGCTGGCAC TCCTGAACAA 
GATGAGTTTT ATGAGAGTGC AGTCGTAGAA ACCAACTGCA TATACAGACT CATGGAGGAT 
AAACTTGTTC CCCATGATGA CTACTGGGGT CGAGTCCCTC GCTGCTCCAT GCTGAACCCT 
AAAGAGGTGC TGGTATTTGA TTTTGGTAGT GAGGTCTATG TATGGCATGG CAAGGAGGTG 
ACGTTGGCAC AGAGAAAAGT GGCATTTCAG CTGGCGAAGC ACTTGTGGAA TGGCACCTTT 
GACTACACCA ACTGTGACAT TAATCCTCTG GACCCTGGAG AGTGCAATGC TCTCATACCC 
AGGAAAGGCC AGGGACGACC AGACTGGGCT GTGTTTGGCA GGTTAACTCA GCACAATGAA 
ACTACTCTTT TTAAAGAGAA GTTTTTGGAC TGGAGTGATT CCAAGAATTC TAAGAAAAAT 
GGTGACTTGC ATGTTGAAAA CAAGGAGCTT CGGAGTGGTG AATGTCGGCC CTATGATGCG 
TCTCTGATGC TATCTGCATT ACGGGCGCCA GTCAGGACCG TTCTGGATGG GGTGAACATT 
GGACGAGGAT ATGGATTAGT GGAAGGTGAA GAGGGACGTA ATTTTGAGAT CAGCACATTG 
TCTGTGGATG TCTGGCATAT TTTAGAGTTT GACTACAGTC GACTACCCAA ACAGAGCATT 
GGTCAGTTTC ATGAAGGTGA CACATACGTG GTCAAATGGA AGTACATGAT CAGCAGAGCA 
GTTGGCAAGA GACTGCATTC AGAAAGAATC ATCGGTCCAG GGAAGGAGAA ATGTTGTTAT 
TTCTTCTGGC AGGGTCGTAA CTCTACGGTG AATGAAAAGG GAACGTCAGC ACTGATGACT 
GTTGAGCTGG ATGAGGAAAG AGGAGCTCAG GTCCAGGTAC AGCAAGGAAA GGAGCCTCCT 
TGTTTCCTAC AGTGCTTCAA TGGAGGAATG ATTGTTCATG CTGGAAAAAG AGAAGAGGAG 
GAAGACAATA CACAAAACGA CTGGCGATTG TACTGTGTGA GAGGAGAGAA ACCCATAGAG 
GGCCATCTGC TGGAGGCAGT GTGTCACTGC AGCAGCTTGC GATCCCGGAC CTCTATGATC 
CTCCTGAACA TCCCCAAAGC CAGCCTATAC CTGTGGCATG GCTGCAAGGC ACAAGTGCAC 
ACACGTGACG TGGGCCGTAC CACTGCAAAC AAAATCAAAG AGCAATGTCC TCTGGAGGCA 
GGGCTTCACA GCAGCAGTAA AGTGTCCATC CAGGAGTGTG ATGAGGGAGC TGAACCACAG 
GGCTTCTGGG AGGCTTTGGG CAAAAGAGAT CGAAAAGCGT ACGACTGCAT GCTGCAGGAT 
CCAGGAAAGT TCAACTTCAC TCCTCGGCTT TTCCAGTTAA GCAGTGCCTC GGGAGAGTTT 
GCAGCAGTGG AGTTTGTATA TCCATCCAGA GAACCAAATT TGGTTAACTC GATGCCTTTC 
CTCCAGGAGG ATTTGTATAC AGCTACTCAG CCAGCCCTGT TTTTGGTGGA TAATCATCAT 
GAAGTTTACC TGTGGCAGGG CTGGTGGCCT CAGGACAGTG AAAGCACAGG CTCAGCTCGC 
ATACGGTGGG ACTCTGACAG GAAGTGTGCA ATGGAAACTG TGCTCCAGTA TAGCAAAGAA 
AAAAATGAGA AAAAGACACC AAAATCCTAC CTGATCCATG CAGGACTGGA GCCTCTTACA 
TTCACCAACA TGTTTCCCAG CTGGGAACAC AGAGAGGACA TCGCTGAAAT CACAGAGAAG 
GAGGCCGAAG TATGTCATCA GATCATTCTT GTTGAAGATG TCTTGAGCCG ACTCTGCAAG 
ACCACTTACC CACTGGCTGA ACTAAAAGCA CGTCCCTTAC CGGAAGGTGT CGACCCTCTA 
CGTCTTGAAA CCTACTTATC AGATGAGGAC TTTGAGGGTG TAGGGGCTTC TGGGAAGAAA 
GCTTTGGAGA TGACGAGAAG TGAATATGAG GCACTGCCTG GATGGAAGCA AGTGAATGTG 
AAGAAAGCCA AAGGATTGTT TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021335691.1
CDS	338..5881
Translation	MYRNPWISNYINHVKLCQGFTRETAPKVGNTKQRRALSFQKEFSFEEKGEGANKEVDVSLLGNTPKVSELRKRFESISTSQSWDMNRKERIARRLEGIDNDVLQSLPGCGGLVSNRLLEEDTPRYTRAADTCDPCTVSLQHYGKSEPSSQSTDVPSRSYAVHPESVFSTGSSHVAELDSKAERIARYKAERRRQLAERYGISLDQEMETDYTTRFSRPSRELDGSDRHHRSKSEGRDGVEQNAYTSSHHVDKSSSDYHSSPEYSRERTDSISERERMMNLENQRRAQERDRLHGGGGVAPDPSAYMDVSGSARVPGREPGMMGVPSSPNSGRRGLMLSPKQGASPGDLFIEQQAHSILQRHGSHLSSEPSSPEVDEEKLDERAKLSVAAKRSLFRELEKTSDGSVLKTWSRNPAVERRLRRGQDRSRTQPVTTEEVVIAATLQASSQQSSMVREQAREVRLAQEATETDRARHASPGEGGHEEPDLSTLTLAEKMALFNRLAQPSSHGSRARGDTRARRSNARYQTQPITLGEVEQFQPAGGARFAPSSNSAMRSSTVSTEHAGDIHLTMPNVSGPAYYEQTVSPPHHPQPAHEIQTHQSLAEGERLLLSTGKRKLPSPDSVKRTPHAQPARDWDSKAVAVTSQDLSQTDGEHTTDLPVHTAAAVSALQSHSLHEDHTREEEQESDFKTEDGDELSDVMSARQMSIKERLALLKKSGEEDWRNRINKKQDVVKVAVSERHAQLWEVEQSFKKKDDGMMIDDFAVSDQLWDPVFSSSLLCASEPLDQIANLPKKMGNEVVESQQLERVEMSIQERKQLITSQEEAWKAKGYGAANDSTQFTVAGRMVKKGLAAPSVLTNPVLSPLSSKNKNTSHTITKPHEEIEVKQNMKLESDMKLEKLETFLGRLNSKVPGLQEATIAVTEKSVKEVMTLDDEVFDKFYRHAEDMAKFTSRVEISDDFDDIFGVQLPRLTSEMVQHKRAVRPTRNVQSSKNPLKMLAVREDIRHEYTEQRLNIGLLESKRMKQEKMNKNSGFSEVALAGLASKENFSSVSLRSVNASEQSSNNSAMPYKKLMLIQVKGRRHVQTRLVEPRASSLNSGDCFLLITPHHCFIWIGEFANVIEKAKAAELATFVQTKHDLGCRASYVQTIEEGANTHTHAAKDFWKILGGQTSYQSAGTPEQDEFYESAVVETNCIYRLMEDKLVPHDDYWGRVPRCSMLNPKEVLVFDFGSEVYVWHGKEVTLAQRKVAFQLAKHLWNGTFDYTNCDINPLDPGECNALIPRKGQGRPDWAVFGRLTQHNETTLFKEKFLDWSDSKNSKKNGDLHVENKELRSGECRPYDASLMLSALRAPVRTVLDGVNIGRGYGLVEGEEGRNFEISTLSVDVWHILEFDYSRLPKQSIGQFHEGDTYVVKWKYMISRAVGKRLHSERIIGPGKEKCCYFFWQGRNSTVNEKGTSALMTVELDEERGAQVQVQQGKEPPCFLQCFNGGMIVHAGKREEEEDNTQNDWRLYCVRGEKPIEGHLLEAVCHCSSLRSRTSMILLNIPKASLYLWHGCKAQVHTRDVGRTTANKIKEQCPLEAGLHSSSKVSIQECDEGAEPQGFWEALGKRDRKAYDCMLQDPGKFNFTPRLFQLSSASGEFAAVEFVYPSREPNLVNSMPFLQEDLYTATQPALFLVDNHHEVYLWQGWWPQDSESTGSARIRWDSDRKCAMETVLQYSKEKNEKKTPKSYLIHAGLEPLTFTNMFPSWEHREDIAEITEKEAEVCHQIILVEDVLSRLCKTTYPLAELKARPLPEGVDPLRLETYLSDEDFEGVGASGKKALEMTRSEYEALPGWKQVNVKKAKGLF

Target ORF information:

RefSeq Version	XM_021480016.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio supervillin a (svila), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021480016.1

ORF Insert Sequence:

ATGTATCGGA ACCCTTGGAT CTCCAACTAT ATCAACCATG TCAAGTTGTG CCAAGGCTTC 
ACCAGAGAAA CAGCCCCTAA AGTTGGCAAT ACCAAGCAAC GGAGAGCTCT TAGTTTTCAG 
AAAGAGTTCT CGTTTGAGGA GAAAGGTGAG GGAGCCAACA AAGAAGTAGA TGTTAGTCTG 
CTGGGGAATA CACCCAAGGT CTCAGAATTA AGGAAACGTT TTGAAAGTAT AAGCACAAGT 
CAGAGTTGGG ACATGAACAG GAAGGAGCGA ATTGCACGGC GCCTGGAAGG AATCGATAAT 
GATGTTCTCC AAAGTCTTCC AGGTTGTGGT GGTCTAGTTT CTAATCGACT GCTGGAGGAG 
GACACTCCAA GATATACCCG TGCAGCAGAC ACCTGTGACC CCTGTACAGT TTCTCTTCAG 
CACTATGGCA AAAGTGAACC AAGTTCACAA AGCACAGATG TTCCATCCAG AAGTTACGCA 
GTCCACCCGG AGTCTGTCTT CAGCACTGGC TCCTCTCATG TGGCAGAGCT GGACTCTAAA 
GCAGAAAGGA TTGCACGCTA CAAGGCTGAA CGCCGCCGTC AGTTAGCAGA GCGCTATGGC 
ATCTCTCTAG ATCAGGAGAT GGAAACAGAC TATACAACTC GCTTCTCCAG ACCCTCCAGA 
GAGCTTGATG GTTCAGACAG ACACCACAGG TCAAAATCAG AAGGGAGAGA TGGAGTCGAG 
CAAAATGCTT ACACCAGCAG CCATCATGTG GATAAGAGCA GCTCAGACTA TCACTCCAGT 
CCAGAGTACA GCCGCGAGCG CACAGACTCC ATCTCAGAAC GTGAAAGAAT GATGAATCTG 
GAGAACCAGA GGAGGGCACA GGAGCGTGAC CGATTACATG GAGGTGGAGG GGTTGCACCT 
GACCCATCTG CGTATATGGA TGTGTCTGGA TCTGCAAGGG TACCAGGAAG GGAGCCAGGG 
ATGATGGGAG TTCCCAGCTC ACCCAATTCA GGCCGAAGGG GCTTGATGCT CTCTCCCAAA 
CAGGGAGCGT CTCCTGGTGA TCTGTTTATT GAGCAGCAGG CTCACAGCAT CCTCCAAAGA 
CACGGGTCTC ATCTAAGCTC TGAACCATCA AGCCCTGAGG TGGATGAAGA GAAACTAGAT 
GAGAGAGCAA AGCTGAGCGT AGCTGCCAAG CGTTCCCTCT TCAGGGAGCT TGAAAAGACT 
TCAGATGGTT CCGTTTTAAA GACATGGAGT CGTAATCCTG CTGTTGAGCG GAGATTGAGG 
AGAGGTCAAG ATCGATCCCG CACTCAGCCA GTCACCACAG AGGAAGTAGT TATTGCTGCT 
ACCTTGCAGG CTTCGTCTCA GCAAAGCTCC ATGGTGAGAG AGCAGGCCAG AGAGGTTCGT 
CTGGCTCAGG AGGCAACGGA GACAGATCGA GCTCGCCACG CTTCTCCGGG GGAAGGCGGA 
CACGAGGAGC CAGACCTTTC CACACTCACT CTAGCAGAAA AGATGGCACT TTTTAACCGT 
CTTGCCCAGC CGTCCAGCCA CGGCTCTAGG GCCAGAGGAG ACACGCGTGC ACGGAGGAGT 
AACGCACGCT ATCAGACTCA ACCAATCACA CTAGGAGAAG TCGAACAGTT TCAGCCTGCA 
GGTGGAGCTC GTTTTGCACC CAGCTCCAAC TCAGCCATGA GATCCTCCAC TGTTTCCACT 
GAACATGCGG GCGACATCCA TCTGACCATG CCAAATGTAT CTGGGCCTGC ATATTATGAG 
CAGACCGTTT CTCCACCGCA TCACCCTCAG CCTGCCCATG AGATCCAGAC ACATCAGAGT 
CTGGCTGAAG GGGAAAGGCT GCTGTTGAGC ACTGGGAAGA GGAAGCTTCC CTCCCCAGAC 
AGCGTGAAAC GGACACCGCA TGCTCAGCCG GCCAGAGACT GGGACAGTAA GGCTGTGGCG 
GTGACCTCTC AAGACCTTTC ACAAACAGAT GGAGAGCACA CAACAGATCT TCCTGTACAC 
ACTGCCGCTG CTGTATCTGC ATTACAGAGC CATTCTCTCC ATGAGGACCA CACCAGAGAG 
GAAGAGCAGG AGAGCGACTT CAAAACAGAA GACGGAGACG AGCTCTCGGA CGTCATGTCT 
GCCAGACAGA TGTCCATCAA AGAGAGGCTG GCTCTGCTGA AGAAGAGTGG AGAGGAAGAC 
TGGAGGAACA GGATAAATAA AAAACAGGAT GTGGTGAAGG TTGCTGTGTC TGAAAGACAT 
GCCCAGCTAT GGGAGGTGGA GCAGAGCTTC AAGAAGAAGG ATGATGGGAT GATGATAGAT 
GACTTTGCTG TGTCTGATCA ACTCTGGGAT CCTGTCTTTT CCTCCTCCCT TTTGTGTGCC 
TCTGAACCAC TGGATCAAAT AGCAAACCTT CCCAAGAAGA TGGGAAATGA AGTTGTGGAA 
AGCCAACAGC TTGAGCGAGT AGAGATGTCT ATTCAGGAAC GCAAGCAGCT CATCACTTCA 
CAAGAGGAGG CCTGGAAAGC CAAAGGTTAC GGAGCAGCCA ACGACTCCAC TCAGTTTACA 
GTGGCTGGAC GTATGGTCAA AAAAGGCCTG GCAGCCCCTT CTGTTCTCAC AAATCCCGTG 
TTGTCTCCTC TGTCTTCTAA AAACAAGAAT ACATCCCATA CCATCACAAA ACCACATGAA 
GAGATTGAAG TCAAACAGAA CATGAAGCTG GAGTCAGACA TGAAGTTAGA AAAGTTGGAA 
ACATTCCTTG GTCGTTTGAA TAGTAAAGTT CCAGGACTTC AGGAGGCCAC AATTGCTGTC 
ACGGAGAAGT CTGTAAAAGA GGTCATGACT CTGGACGATG AGGTCTTTGA TAAGTTTTAT 
CGACATGCAG AGGATATGGC AAAGTTCACC AGCAGAGTGG AAATTAGTGA TGATTTTGAT 
GACATCTTTG GAGTACAGCT GCCCAGGTTG ACTTCGGAAA TGGTGCAGCA CAAGCGGGCA 
GTTCGGCCTA CCAGAAATGT TCAGTCATCT AAAAACCCGC TGAAAATGCT GGCTGTTCGG 
GAGGACATCA GACATGAGTA CACGGAACAA AGACTGAACA TCGGCCTTCT GGAGAGCAAG 
AGGATGAAAC AGGAAAAGAT GAATAAGAAC AGCGGTTTCT CTGAGGTTGC TCTCGCCGGA 
TTGGCCAGTA AGGAGAACTT CAGCAGTGTG AGTCTGCGAA GTGTGAATGC CTCTGAACAA 
AGCTCCAACA ACAGTGCCAT GCCCTATAAA AAACTCATGC TCATTCAGGT CAAAGGTCGC 
AGGCACGTTC AGACCAGACT GGTTGAGCCC AGAGCTTCCT CCTTGAACAG TGGCGACTGC 
TTCCTCCTGA TCACACCACA TCATTGCTTC ATCTGGATTG GAGAATTCGC TAATGTTATT 
GAGAAGGCCA AAGCTGCAGA GTTGGCCACG TTCGTTCAAA CCAAGCATGA CCTGGGATGC 
AGAGCCTCGT ATGTTCAGAC AATTGAGGAG GGTGCAAACA CTCACACACA CGCTGCCAAA 
GACTTTTGGA AGATTCTCGG TGGCCAAACC AGTTATCAGT CCGCTGGCAC TCCTGAACAA 
GATGAGTTTT ATGAGAGTGC AGTCGTAGAA ACCAACTGCA TATACAGACT CATGGAGGAT 
AAACTTGTTC CCCATGATGA CTACTGGGGT CGAGTCCCTC GCTGCTCCAT GCTGAACCCT 
AAAGAGGTGC TGGTATTTGA TTTTGGTAGT GAGGTCTATG TATGGCATGG CAAGGAGGTG 
ACGTTGGCAC AGAGAAAAGT GGCATTTCAG CTGGCGAAGC ACTTGTGGAA TGGCACCTTT 
GACTACACCA ACTGTGACAT TAATCCTCTG GACCCTGGAG AGTGCAATGC TCTCATACCC 
AGGAAAGGCC AGGGACGACC AGACTGGGCT GTGTTTGGCA GGTTAACTCA GCACAATGAA 
ACTACTCTTT TTAAAGAGAA GTTTTTGGAC TGGAGTGATT CCAAGAATTC TAAGAAAAAT 
GGTGACTTGC ATGTTGAAAA CAAGGAGCTT CGGAGTGGTG AATGTCGGCC CTATGATGCG 
TCTCTGATGC TATCTGCATT ACGGGCGCCA GTCAGGACCG TTCTGGATGG GGTGAACATT 
GGACGAGGAT ATGGATTAGT GGAAGGTGAA GAGGGACGTA ATTTTGAGAT CAGCACATTG 
TCTGTGGATG TCTGGCATAT TTTAGAGTTT GACTACAGTC GACTACCCAA ACAGAGCATT 
GGTCAGTTTC ATGAAGGTGA CACATACGTG GTCAAATGGA AGTACATGAT CAGCAGAGCA 
GTTGGCAAGA GACTGCATTC AGAAAGAATC ATCGGTCCAG GGAAGGAGAA ATGTTGTTAT 
TTCTTCTGGC AGGGTCGTAA CTCTACGGTG AATGAAAAGG GAACGTCAGC ACTGATGACT 
GTTGAGCTGG ATGAGGAAAG AGGAGCTCAG GTCCAGGTAC AGCAAGGAAA GGAGCCTCCT 
TGTTTCCTAC AGTGCTTCAA TGGAGGAATG ATTGTTCATG CTGGAAAAAG AGAAGAGGAG 
GAAGACAATA CACAAAACGA CTGGCGATTG TACTGTGTGA GAGGAGAGAA ACCCATAGAG 
GGCCATCTGC TGGAGGCAGT GTGTCACTGC AGCAGCTTGC GATCCCGGAC CTCTATGATC 
CTCCTGAACA TCCCCAAAGC CAGCCTATAC CTGTGGCATG GCTGCAAGGC ACAAGTGCAC 
ACACGTGACG TGGGCCGTAC CACTGCAAAC AAAATCAAAG AGCAATGTCC TCTGGAGGCA 
GGGCTTCACA GCAGCAGTAA AGTGTCCATC CAGGAGTGTG ATGAGGGAGC TGAACCACAG 
GGCTTCTGGG AGGCTTTGGG CAAAAGAGAT CGAAAAGCGT ACGACTGCAT GCTGCAGGAT 
CCAGGAAAGT TCAACTTCAC TCCTCGGCTT TTCCAGTTAA GCAGTGCCTC GGGAGAGTTT 
GCAGCAGTGG AGTTTGTATA TCCATCCAGA GAACCAAATT TGGTTAACTC GATGCCTTTC 
CTCCAGGAGG ATTTGTATAC AGCTACTCAG CCAGCCCTGT TTTTGGTGGA TAATCATCAT 
GAAGTTTACC TGTGGCAGGG CTGGTGGCCT CAGGACAGTG AAAGCACAGG CTCAGCTCGC 
ATACGGTGGG ACTCTGACAG GAAGTGTGCA ATGGAAACTG TGCTCCAGTA TAGCAAAGAA 
AAAAATGAGA AAAAGACACC AAAATCCTAC CTGATCCATG CAGGACTGGA GCCTCTTACA 
TTCACCAACA TGTTTCCCAG CTGGGAACAC AGAGAGGACA TCGCTGAAAT CACAGAGAAG 
GAGGCCGAAG TATGTCATCA GATCATTCTT GTTGAAGATG TCTTGAGCCG ACTCTGCAAG 
ACCACTTACC CACTGGCTGA ACTAAAAGCA CGTCCCTTAC CGGAAGGTGT CGACCCTCTA 
CGTCTTGAAA CCTACTTATC AGATGAGGAC TTTGAGGGTG TAGGGGCTTC TGGGAAGAAA 
GCTTTGGAGA TGACGAGAAG TGAATATGAG GCACTGCCTG GATGGAAGCA AGTGAATGTG 
AAGAAAGCCA AAGGATTGTT TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

svila ( NM_001035261.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_001030338.2 Danio rerio supervillin a (svila), mRNA.

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CloneID	ODa32155
Clone ID Related Accession (Same CDS sequence)	NM_001035261.2
Accession Version	NM_001035261.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5292bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-25
Organism	Danio rerio(zebrafish)
Product	supervillin
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from DQ227765.1 and CU914155.16. On Jul 22, 2010 this sequence version replaced NM_001035261.1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. ##Evidence-Data-START## Transcript exon combination :: DQ227765.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA4476740, SAMEA4476746 [ECO:0000348] ##Evidence-Data-END##

ATGAACAGGA AGGAGCGAAT TGCACGGCGC CTGGAAGGAA TCGATAATGA TGTTCTCCAA 
AGTCTTCCAG GTTGTGGTGG TCTAGTTTCT AATCGACTGC TGGAGGAGGA CACTCCAAGA 
TATACCCGTG CAGCAGACAC CTGTGACCCC TGTACAGTTT CTCTTCAGCA CTATGGCAAA 
AGTGAACCAA GTTCACAAAG CACAGATGTT CCATCCAGAA GTTACGCAGT CCACCCGGAG 
TCTGTCTTCA GCACTGGCTC CTCTCATGTG GCAGAGCTGG ACTCTAAAGC AGAAAGGATT 
GCACGCTACA AGGCTGAACG CCGCCGTCAG TTAGCAGAGC GCTATGGCAT CTCTCTAGAT 
CAGGAGATGG AAACAGACTA TACAACTCGC TTCTCCAGAC CCTCCAGAGA GCTTGATGGT 
TCAGACAGAC ACCACAGGTC AAAATCAGAA GGGAGAGATG GAGTCGAGCA AAATGCTTAC 
ACCAGCAGCC ATCATGTGGA TAAGAGCAGC TCAGACTATC ACTCCAGTCC AGAGTACAGC 
CGCGAGCGCA CAGACTCCAT CTCAGAACGT GAAAGAATGA TGAATCTGGA GAACCAGAGG 
AGGGCACAGG AGCGTGACCG ATTACATGGA GGTGGAGGGG TTGCACCTGA CCCATCTGCG 
TATATGGATG TGTCTGGATC TGCAAGGGTA CCAGGAAGGG AGCCAGGGAT GATGGGAGTT 
CCCAGCTCAC CCAATTCAGG CCGAAGGGGC TTGATGCTCT CTCCCAAACA GGGAGCGTCT 
CCTGGTGATC TGTTTATTGA GCAGCAGGCT CACAGCATCC TCCAAAGACA CGGGTCTCAT 
CTAAGCTCTG AACCATCAAG CCCTGAGGTG GATGAAGAGA AACTAGATGA GAGAGCAAAG 
CTGAGCGTAG CTGCCAAGCG TTCCCTCTTC AGGGAGCTTG AAAAGACTTC AGATGGTTCC 
GTTTTAAAGA CATGGAGTCG TAATCCTGCT GTTGAGCGGA GATTGAGGAG AGGTCAAGAT 
CGATCCCGCA CTCAGCCAGT CACCACAGAG GAAGTAGTTA TTGCTGCTAC CTTGCAGGCT 
TCGTCTCAGC AAAGCTCCAT GGTGAGAGAG CAGGCCAGAG AGGTTCGTCT GGCTCAGGAG 
GCAACGGAGA CAGATCGAGC TCGCCACGCT TCTCCGGGGG AAGGCGGACA CGAGGAGCCA 
GACCTTTCCA CACTCACTCT AGCAGAAAAG ATGGCACTTT TTAACCGTCT TGCCCAGCCG 
TCCAGCCACG GCTCTAGGGC CAGAGGAGAC ACGCGTGCAC GGAGGAGTAA CGCACGCTAT 
CAGACTCAAC CAATCACACT AGGAGAAGTC GAACAGTTTC AGCCTGCAGG TGGAGCTCGT 
TTTGCACCCA GCTCCAACTC AGCCATGAGA TCCTCCACTG TTTCCACTGA ACATGCGGGC 
GACATCCATC TGACCATGCC AAATGTATCT GGGCCTGCAT ATTATGAGCA GACCGTTTCT 
CCACCGCATC ACCCTCAGCC TGCCCATGAG ATCCAGACAC ATCAGAGTCT GGCTGAAGGG 
GAAAGGCTGC TGTTGAGCAC TGGGAAGAGG AAGCTTCCCT CCCCAGACAG CGTGAAACGG 
ACACCGCATG CTCAGCCGGC CAGAGACTGG GACAGTAAGG CTGTGGCGGT GACCTCTCAA 
GACCTTTCAC AAACAGATGG AGAGCACACA ACAGATCTTC CTGTACACAC TGCCGCTGCT 
GTATCTGCAT TACAGAGCCA TTCTCTCCAT GAGGACCACA CCAGAGAGGA AGAGCAGGAG 
AGCGACTTCA AAACAGAAGA CGGAGACGAG CTCTCGGACG TCATGTCTGC CAGACAGATG 
TCCATCAAAG AGAGGCTGGC TCTGCTGAAG AAGAGTGGAG AGGAAGACTG GAGGAACAGG 
ATAAATAAAA AACAGGATGT GGTGAAGGTT GCTGTGTCTG AAAGACATGC CCAGCTATGG 
GAGGTGGAGC AGAGCTTCAA GAAGAAGGAT GATGGGATGA TGATAGATGA CTTTGCTGTG 
TCTGATCAAC TCTGGGATCC TGTCTTTTCC TCCTCCCTTT TGTGTGCCTC TGAACCACTG 
GATCAAATAG CAAACCTTCC CAAGAAGATG GGAAATGAAG TTGTGGAAAG CCAACAGCTT 
GAGCGAGTAG AGATGTCTAT TCAGGAACGC AAGCAGCTCA TCACTTCACA AGAGGAGGCC 
TGGAAAGCCA AAGGTTACGG AGCAGCCAAC GACTCCACTC AGTTTACAGT GGCTGGACGT 
ATGGTCAAAA AAGGCCTGGC AGCCCCTTCT GTTCTCACAA ATCCCGTGTT GTCTCCTCTG 
TCTTCTAAAA ACAAGAATAC ATCCCATACC ATCACAAAAC CACATGAAGA GATTGAAGTC 
AAACAGAACA TGAAGCTGGA GTCAGACATG AAGTTAGAAA AGTTGGAAAC ATTCCTTGGT 
CGTTTGAATA GTAAAGTTCC AGGACTTCAG GAGGCCACAA TTGCTGTCAC GGAGAAGTCT 
GTAAAAGAGG TCATGACTCT GGACGATGAG GTCTTTGATA AGTTTTATCG ACATGCAGAG 
GATATGGCAA AGTTCACCAG CAGAGTGGAA ATTAGTGATG ATTTTGATGA CATCTTTGGA 
GTACAGCTGC CCAGGTTGAC TTCGGAAATG GTGCAGCACA AGCGGGCAGT TCGGCCTACC 
AGAAATGTTC AGTCATCTAA AAACCCGCTG AAAATGCTGG CTGTTCGGGA GGACATCAGA 
CATGAGTACA CGGAACAAAG ACTGAACATC GGCCTTCTGG AGAGCAAGAG GATGAAACAG 
GAAAAGATGA ATAAGAACAG CGGTTTCTCT GAGGTTGCTC TCGCCGGATT GGCCAGTAAG 
GAGAACTTCA GCAGTGTGAG TCTGCGAAGT GTGAATGCCT CTGAACAAAG CTCCAACAAC 
AGTGCCATGC CCTATAAAAA ACTCATGCTC ATTCAGGTCA AAGGTCGCAG GCACGTTCAG 
ACCAGACTGG TTGAGCCCAG AGCTTCCTCC TTGAACAGTG GCGACTGCTT CCTCCTGATC 
ACACCACATC ATTGCTTCAT CTGGATTGGA GAATTCGCTA ATGTTATTGA GAAGGCCAAA 
GCTGCAGAGT TGGCCACGTT CGTTCAAACC AAGCATGACC TGGGATGCAG AGCCTCGTAT 
GTTCAGACAA TTGAGGAGGG TGCAAACACT CACACACACG CTGCCAAAGA CTTTTGGAAG 
ATTCTCGGTG GCCAAACCAG TTATCAGTCC GCTGGCACTC CTGAACAAGA TGAGTTTTAT 
GAGAGTGCAG TCGTAGAAAC CAACTGCATA TACAGACTCA TGGAGGATAA ACTTGTTCCC 
CATGATGACT ACTGGGGTCG AGTCCCTCGC TGCTCCATGC TGAACCCTAA AGAGGTGCTG 
GTATTTGATT TTGGTAGTGA GGTCTATGTA TGGCATGGCA AGGAGGTGAC GTTGGCACAG 
AGAAAAGTGG CATTTCAGCT GGCGAAGCAC TTGTGGAATG GCACCTTTGA CTACACCAAC 
TGTGACATTA ATCCTCTGGA CCCTGGAGAG TGCAATGCTC TCATACCCAG GAAAGGCCAG 
GGACGACCAG ACTGGGCTGT GTTTGGCAGG TTAACTCAGC ACAATGAAAC TACTCTTTTT 
AAAGAGAAGT TTTTGGACTG GAGTGATTCC AAGAATTCTA AGAAAAATGG TGACTTGCAT 
GTTGAAAACA AGGAGCTTCG GAGTGGTGAA TGTCGGCCCT ATGATGCGTC TCTGATGCTA 
TCTGCATTAC GGGCGCCAGT CAGGACCGTT CTGGATGGGG TGAACATTGG ACGAGGATAT 
GGATTAGTGG AAGGTGAAGA GGGACGTAAT TTTGAGATCA GCACATTGTC TGTGGATGTC 
TGGCATATTT TAGAGTTTGA CTACAGTCGA CTACCCAAAC AGAGCATTGG TCAGTTTCAT 
GAAGGTGACA CATACGTGGT CAAATGGAAG TACATGATCA GCAGAGCAGT TGGCAAGAGA 
CTGCATTCAG AAAGAATCAT CGGTCCAGGG AAGGAGAAAT GTTGTTATTT CTTCTGGCAG 
GGTCGTAACT CTACGGTGAA TGAAAAGGGA ACGTCAGCAC TGATGACTGT TGAGCTGGAT 
GAGGAAAGAG GAGCTCAGGT CCAGGTACAG CAAGGAAAGG AGCCTCCTTG TTTCCTACAG 
TGCTTCAATG GAGGAATGAT TGTTCATGCT GGAAAAAGAG AAGAGGAGGA AGACAATACA 
CAAAACGACT GGCGATTGTA CTGTGTGAGA GGAGAGAAAC CCATAGAGGG CCATCTGCTG 
GAGGCAGTGT GTCACTGCAG CAGCTTGCGA TCCCGGACCT CTATGATCCT CCTGAACATC 
CCCAAAGCCA GCCTATACCT GTGGCATGGC TGCAAGGCAC AAGTGCACAC ACGTGACGTG 
GGCCGTACCA CTGCAAACAA AATCAAAGAG CAATGTCCTC TGGAGGCAGG GCTTCACAGC 
AGCAGTAAAG TGTCCATCCA GGAGTGTGAT GAGGGAGCTG AACCACAGGG CTTCTGGGAG 
GCTTTGGGCA AAAGAGATCG AAAAGCGTAC GACTGCATGC TGCAGGATCC AGGAAAGTTC 
AACTTCACTC CTCGGCTTTT CCAGTTAAGC AGTGCCTCGG GAGAGTTTGC AGCAGTGGAG 
TTTGTATATC CATCCAGAGA ACCAAATTTG GTTAACTCGA TGCCTTTCCT CCAGGAGGAT 
TTGTATACAG CTACTCAGCC AGCCCTGTTT TTGGTGGATA ATCATCATGA AGTTTACCTG 
TGGCAGGGCT GGTGGCCTCA GGACAGTGAA AGCACAGGCT CAGCTCGCAT ACGGTGGGAC 
TCTGACAGGA AGTGTGCAAT GGAAACTGTG CTCCAGTATA GCAAAGAAAA AAATGAGAAA 
AAGACACCAA AATCCTACCT GATCCATGCA GGACTGGAGC CTCTTACATT CACCAACATG 
TTTCCCAGCT GGGAACACAG AGAGGACATC GCTGAAATCA CAGAGAAGGA GGCCGAAGTA 
TGTCATCAGA TCATTCTTGT TGAAGATGTC TTGAGCCGAC TCTGCAAGAC CACTTACCCA 
CTGGCTGAAC TAAAAGCACG TCCCTTACCG GAAGGTGTCG ACCCTCTACG TCTTGAAACC 
TACTTATCAG ATGAGGACTT TGAGGGTGTA GGGGCTTCTG GGAAGAAAGC TTTGGAGATG 
ACGAGAAGTG AATATGAGGC ACTGCCTGGA TGGAAGCAAG TGAATGTGAA GAAAGCCAAA 
GGATTGTTTT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001030338.2
CDS	352..5643
Translation	MNRKERIARRLEGIDNDVLQSLPGCGGLVSNRLLEEDTPRYTRAADTCDPCTVSLQHYGKSEPSSQSTDVPSRSYAVHPESVFSTGSSHVAELDSKAERIARYKAERRRQLAERYGISLDQEMETDYTTRFSRPSRELDGSDRHHRSKSEGRDGVEQNAYTSSHHVDKSSSDYHSSPEYSRERTDSISERERMMNLENQRRAQERDRLHGGGGVAPDPSAYMDVSGSARVPGREPGMMGVPSSPNSGRRGLMLSPKQGASPGDLFIEQQAHSILQRHGSHLSSEPSSPEVDEEKLDERAKLSVAAKRSLFRELEKTSDGSVLKTWSRNPAVERRLRRGQDRSRTQPVTTEEVVIAATLQASSQQSSMVREQAREVRLAQEATETDRARHASPGEGGHEEPDLSTLTLAEKMALFNRLAQPSSHGSRARGDTRARRSNARYQTQPITLGEVEQFQPAGGARFAPSSNSAMRSSTVSTEHAGDIHLTMPNVSGPAYYEQTVSPPHHPQPAHEIQTHQSLAEGERLLLSTGKRKLPSPDSVKRTPHAQPARDWDSKAVAVTSQDLSQTDGEHTTDLPVHTAAAVSALQSHSLHEDHTREEEQESDFKTEDGDELSDVMSARQMSIKERLALLKKSGEEDWRNRINKKQDVVKVAVSERHAQLWEVEQSFKKKDDGMMIDDFAVSDQLWDPVFSSSLLCASEPLDQIANLPKKMGNEVVESQQLERVEMSIQERKQLITSQEEAWKAKGYGAANDSTQFTVAGRMVKKGLAAPSVLTNPVLSPLSSKNKNTSHTITKPHEEIEVKQNMKLESDMKLEKLETFLGRLNSKVPGLQEATIAVTEKSVKEVMTLDDEVFDKFYRHAEDMAKFTSRVEISDDFDDIFGVQLPRLTSEMVQHKRAVRPTRNVQSSKNPLKMLAVREDIRHEYTEQRLNIGLLESKRMKQEKMNKNSGFSEVALAGLASKENFSSVSLRSVNASEQSSNNSAMPYKKLMLIQVKGRRHVQTRLVEPRASSLNSGDCFLLITPHHCFIWIGEFANVIEKAKAAELATFVQTKHDLGCRASYVQTIEEGANTHTHAAKDFWKILGGQTSYQSAGTPEQDEFYESAVVETNCIYRLMEDKLVPHDDYWGRVPRCSMLNPKEVLVFDFGSEVYVWHGKEVTLAQRKVAFQLAKHLWNGTFDYTNCDINPLDPGECNALIPRKGQGRPDWAVFGRLTQHNETTLFKEKFLDWSDSKNSKKNGDLHVENKELRSGECRPYDASLMLSALRAPVRTVLDGVNIGRGYGLVEGEEGRNFEISTLSVDVWHILEFDYSRLPKQSIGQFHEGDTYVVKWKYMISRAVGKRLHSERIIGPGKEKCCYFFWQGRNSTVNEKGTSALMTVELDEERGAQVQVQQGKEPPCFLQCFNGGMIVHAGKREEEEDNTQNDWRLYCVRGEKPIEGHLLEAVCHCSSLRSRTSMILLNIPKASLYLWHGCKAQVHTRDVGRTTANKIKEQCPLEAGLHSSSKVSIQECDEGAEPQGFWEALGKRDRKAYDCMLQDPGKFNFTPRLFQLSSASGEFAAVEFVYPSREPNLVNSMPFLQEDLYTATQPALFLVDNHHEVYLWQGWWPQDSESTGSARIRWDSDRKCAMETVLQYSKEKNEKKTPKSYLIHAGLEPLTFTNMFPSWEHREDIAEITEKEAEVCHQIILVEDVLSRLCKTTYPLAELKARPLPEGVDPLRLETYLSDEDFEGVGASGKKALEMTRSEYEALPGWKQVNVKKAKGLF

Target ORF information:

RefSeq Version	NM_001035261.2
Organism	Danio rerio(zebrafish)
Definition	Danio rerio supervillin a (svila), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001035261.2

ORF Insert Sequence:

ATGAACAGGA AGGAGCGAAT TGCACGGCGC CTGGAAGGAA TCGATAATGA TGTTCTCCAA 
AGTCTTCCAG GTTGTGGTGG TCTAGTTTCT AATCGACTGC TGGAGGAGGA CACTCCAAGA 
TATACCCGTG CAGCAGACAC CTGTGACCCC TGTACAGTTT CTCTTCAGCA CTATGGCAAA 
AGTGAACCAA GTTCACAAAG CACAGATGTT CCATCCAGAA GTTACGCAGT CCACCCGGAG 
TCTGTCTTCA GCACTGGCTC CTCTCATGTG GCAGAGCTGG ACTCTAAAGC AGAAAGGATT 
GCACGCTACA AGGCTGAACG CCGCCGTCAG TTAGCAGAGC GCTATGGCAT CTCTCTAGAT 
CAGGAGATGG AAACAGACTA TACAACTCGC TTCTCCAGAC CCTCCAGAGA GCTTGATGGT 
TCAGACAGAC ACCACAGGTC AAAATCAGAA GGGAGAGATG GAGTCGAGCA AAATGCTTAC 
ACCAGCAGCC ATCATGTGGA TAAGAGCAGC TCAGACTATC ACTCCAGTCC AGAGTACAGC 
CGCGAGCGCA CAGACTCCAT CTCAGAACGT GAAAGAATGA TGAATCTGGA GAACCAGAGG 
AGGGCACAGG AGCGTGACCG ATTACATGGA GGTGGAGGGG TTGCACCTGA CCCATCTGCG 
TATATGGATG TGTCTGGATC TGCAAGGGTA CCAGGAAGGG AGCCAGGGAT GATGGGAGTT 
CCCAGCTCAC CCAATTCAGG CCGAAGGGGC TTGATGCTCT CTCCCAAACA GGGAGCGTCT 
CCTGGTGATC TGTTTATTGA GCAGCAGGCT CACAGCATCC TCCAAAGACA CGGGTCTCAT 
CTAAGCTCTG AACCATCAAG CCCTGAGGTG GATGAAGAGA AACTAGATGA GAGAGCAAAG 
CTGAGCGTAG CTGCCAAGCG TTCCCTCTTC AGGGAGCTTG AAAAGACTTC AGATGGTTCC 
GTTTTAAAGA CATGGAGTCG TAATCCTGCT GTTGAGCGGA GATTGAGGAG AGGTCAAGAT 
CGATCCCGCA CTCAGCCAGT CACCACAGAG GAAGTAGTTA TTGCTGCTAC CTTGCAGGCT 
TCGTCTCAGC AAAGCTCCAT GGTGAGAGAG CAGGCCAGAG AGGTTCGTCT GGCTCAGGAG 
GCAACGGAGA CAGATCGAGC TCGCCACGCT TCTCCGGGGG AAGGCGGACA CGAGGAGCCA 
GACCTTTCCA CACTCACTCT AGCAGAAAAG ATGGCACTTT TTAACCGTCT TGCCCAGCCG 
TCCAGCCACG GCTCTAGGGC CAGAGGAGAC ACGCGTGCAC GGAGGAGTAA CGCACGCTAT 
CAGACTCAAC CAATCACACT AGGAGAAGTC GAACAGTTTC AGCCTGCAGG TGGAGCTCGT 
TTTGCACCCA GCTCCAACTC AGCCATGAGA TCCTCCACTG TTTCCACTGA ACATGCGGGC 
GACATCCATC TGACCATGCC AAATGTATCT GGGCCTGCAT ATTATGAGCA GACCGTTTCT 
CCACCGCATC ACCCTCAGCC TGCCCATGAG ATCCAGACAC ATCAGAGTCT GGCTGAAGGG 
GAAAGGCTGC TGTTGAGCAC TGGGAAGAGG AAGCTTCCCT CCCCAGACAG CGTGAAACGG 
ACACCGCATG CTCAGCCGGC CAGAGACTGG GACAGTAAGG CTGTGGCGGT GACCTCTCAA 
GACCTTTCAC AAACAGATGG AGAGCACACA ACAGATCTTC CTGTACACAC TGCCGCTGCT 
GTATCTGCAT TACAGAGCCA TTCTCTCCAT GAGGACCACA CCAGAGAGGA AGAGCAGGAG 
AGCGACTTCA AAACAGAAGA CGGAGACGAG CTCTCGGACG TCATGTCTGC CAGACAGATG 
TCCATCAAAG AGAGGCTGGC TCTGCTGAAG AAGAGTGGAG AGGAAGACTG GAGGAACAGG 
ATAAATAAAA AACAGGATGT GGTGAAGGTT GCTGTGTCTG AAAGACATGC CCAGCTATGG 
GAGGTGGAGC AGAGCTTCAA GAAGAAGGAT GATGGGATGA TGATAGATGA CTTTGCTGTG 
TCTGATCAAC TCTGGGATCC TGTCTTTTCC TCCTCCCTTT TGTGTGCCTC TGAACCACTG 
GATCAAATAG CAAACCTTCC CAAGAAGATG GGAAATGAAG TTGTGGAAAG CCAACAGCTT 
GAGCGAGTAG AGATGTCTAT TCAGGAACGC AAGCAGCTCA TCACTTCACA AGAGGAGGCC 
TGGAAAGCCA AAGGTTACGG AGCAGCCAAC GACTCCACTC AGTTTACAGT GGCTGGACGT 
ATGGTCAAAA AAGGCCTGGC AGCCCCTTCT GTTCTCACAA ATCCCGTGTT GTCTCCTCTG 
TCTTCTAAAA ACAAGAATAC ATCCCATACC ATCACAAAAC CACATGAAGA GATTGAAGTC 
AAACAGAACA TGAAGCTGGA GTCAGACATG AAGTTAGAAA AGTTGGAAAC ATTCCTTGGT 
CGTTTGAATA GTAAAGTTCC AGGACTTCAG GAGGCCACAA TTGCTGTCAC GGAGAAGTCT 
GTAAAAGAGG TCATGACTCT GGACGATGAG GTCTTTGATA AGTTTTATCG ACATGCAGAG 
GATATGGCAA AGTTCACCAG CAGAGTGGAA ATTAGTGATG ATTTTGATGA CATCTTTGGA 
GTACAGCTGC CCAGGTTGAC TTCGGAAATG GTGCAGCACA AGCGGGCAGT TCGGCCTACC 
AGAAATGTTC AGTCATCTAA AAACCCGCTG AAAATGCTGG CTGTTCGGGA GGACATCAGA 
CATGAGTACA CGGAACAAAG ACTGAACATC GGCCTTCTGG AGAGCAAGAG GATGAAACAG 
GAAAAGATGA ATAAGAACAG CGGTTTCTCT GAGGTTGCTC TCGCCGGATT GGCCAGTAAG 
GAGAACTTCA GCAGTGTGAG TCTGCGAAGT GTGAATGCCT CTGAACAAAG CTCCAACAAC 
AGTGCCATGC CCTATAAAAA ACTCATGCTC ATTCAGGTCA AAGGTCGCAG GCACGTTCAG 
ACCAGACTGG TTGAGCCCAG AGCTTCCTCC TTGAACAGTG GCGACTGCTT CCTCCTGATC 
ACACCACATC ATTGCTTCAT CTGGATTGGA GAATTCGCTA ATGTTATTGA GAAGGCCAAA 
GCTGCAGAGT TGGCCACGTT CGTTCAAACC AAGCATGACC TGGGATGCAG AGCCTCGTAT 
GTTCAGACAA TTGAGGAGGG TGCAAACACT CACACACACG CTGCCAAAGA CTTTTGGAAG 
ATTCTCGGTG GCCAAACCAG TTATCAGTCC GCTGGCACTC CTGAACAAGA TGAGTTTTAT 
GAGAGTGCAG TCGTAGAAAC CAACTGCATA TACAGACTCA TGGAGGATAA ACTTGTTCCC 
CATGATGACT ACTGGGGTCG AGTCCCTCGC TGCTCCATGC TGAACCCTAA AGAGGTGCTG 
GTATTTGATT TTGGTAGTGA GGTCTATGTA TGGCATGGCA AGGAGGTGAC GTTGGCACAG 
AGAAAAGTGG CATTTCAGCT GGCGAAGCAC TTGTGGAATG GCACCTTTGA CTACACCAAC 
TGTGACATTA ATCCTCTGGA CCCTGGAGAG TGCAATGCTC TCATACCCAG GAAAGGCCAG 
GGACGACCAG ACTGGGCTGT GTTTGGCAGG TTAACTCAGC ACAATGAAAC TACTCTTTTT 
AAAGAGAAGT TTTTGGACTG GAGTGATTCC AAGAATTCTA AGAAAAATGG TGACTTGCAT 
GTTGAAAACA AGGAGCTTCG GAGTGGTGAA TGTCGGCCCT ATGATGCGTC TCTGATGCTA 
TCTGCATTAC GGGCGCCAGT CAGGACCGTT CTGGATGGGG TGAACATTGG ACGAGGATAT 
GGATTAGTGG AAGGTGAAGA GGGACGTAAT TTTGAGATCA GCACATTGTC TGTGGATGTC 
TGGCATATTT TAGAGTTTGA CTACAGTCGA CTACCCAAAC AGAGCATTGG TCAGTTTCAT 
GAAGGTGACA CATACGTGGT CAAATGGAAG TACATGATCA GCAGAGCAGT TGGCAAGAGA 
CTGCATTCAG AAAGAATCAT CGGTCCAGGG AAGGAGAAAT GTTGTTATTT CTTCTGGCAG 
GGTCGTAACT CTACGGTGAA TGAAAAGGGA ACGTCAGCAC TGATGACTGT TGAGCTGGAT 
GAGGAAAGAG GAGCTCAGGT CCAGGTACAG CAAGGAAAGG AGCCTCCTTG TTTCCTACAG 
TGCTTCAATG GAGGAATGAT TGTTCATGCT GGAAAAAGAG AAGAGGAGGA AGACAATACA 
CAAAACGACT GGCGATTGTA CTGTGTGAGA GGAGAGAAAC CCATAGAGGG CCATCTGCTG 
GAGGCAGTGT GTCACTGCAG CAGCTTGCGA TCCCGGACCT CTATGATCCT CCTGAACATC 
CCCAAAGCCA GCCTATACCT GTGGCATGGC TGCAAGGCAC AAGTGCACAC ACGTGACGTG 
GGCCGTACCA CTGCAAACAA AATCAAAGAG CAATGTCCTC TGGAGGCAGG GCTTCACAGC 
AGCAGTAAAG TGTCCATCCA GGAGTGTGAT GAGGGAGCTG AACCACAGGG CTTCTGGGAG 
GCTTTGGGCA AAAGAGATCG AAAAGCGTAC GACTGCATGC TGCAGGATCC AGGAAAGTTC 
AACTTCACTC CTCGGCTTTT CCAGTTAAGC AGTGCCTCGG GAGAGTTTGC AGCAGTGGAG 
TTTGTATATC CATCCAGAGA ACCAAATTTG GTTAACTCGA TGCCTTTCCT CCAGGAGGAT 
TTGTATACAG CTACTCAGCC AGCCCTGTTT TTGGTGGATA ATCATCATGA AGTTTACCTG 
TGGCAGGGCT GGTGGCCTCA GGACAGTGAA AGCACAGGCT CAGCTCGCAT ACGGTGGGAC 
TCTGACAGGA AGTGTGCAAT GGAAACTGTG CTCCAGTATA GCAAAGAAAA AAATGAGAAA 
AAGACACCAA AATCCTACCT GATCCATGCA GGACTGGAGC CTCTTACATT CACCAACATG 
TTTCCCAGCT GGGAACACAG AGAGGACATC GCTGAAATCA CAGAGAAGGA GGCCGAAGTA 
TGTCATCAGA TCATTCTTGT TGAAGATGTC TTGAGCCGAC TCTGCAAGAC CACTTACCCA 
CTGGCTGAAC TAAAAGCACG TCCCTTACCG GAAGGTGTCG ACCCTCTACG TCTTGAAACC 
TACTTATCAG ATGAGGACTT TGAGGGTGTA GGGGCTTCTG GGAAGAAAGC TTTGGAGATG 
ACGAGAAGTG AATATGAGGC ACTGCCTGGA TGGAAGCAAG TGAATGTGAA GAAAGCCAAA 
GGATTGTTTT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

svila ( XM_021480011.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021335686.1 Danio rerio supervillin a (svila), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa148129
Clone ID Related Accession (Same CDS sequence)	XM_021480011.1
Accession Version	XM_021480011.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6795bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	supervillin isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007123.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTATCGGA ACCCTTGGAT CTCCAACTAT ATCAACCATG TCAAGTTGTG CCAAGGCTTC 
ACCAGAGAAA CAGCCCCTAA AGTTGGCAAT ACCAAGCAAC GGAGAGCTCT TAGTTTTCAG 
AAAGAGTTCT CGTTTGAGGA GAAAGGTGAG GGAGCCAACA AAGAAGTAGA TGTTAGTCTG 
CTGGGGAATA CACCCAAGGT CTCAGAATTA AGGAAACGTT TTGAAAGTAT AAGCACAAGT 
CAGAGTTGGG ACATGAACAG GAAGGAGCGA ATTGCACGGC GCCTGGAAGG AATCGATAAT 
GATGTTCTCC AAAGTCTTCC AGGTTGTGGT GGTCTAGTTT CTAATCGACT GCTGGAGGAG 
GACACTCCAA GATATACCCG TGCAGCAGAC ACCTGTGACC CCTGTACAGT TTCTCTTCAG 
CACTATGGCA AAAGTGAACC AAGTTCACAA AGCACAGATG TTCCATCCAG AAGTTACGCA 
GTCCACCCGG AGTCTGTCTT CAGCACTGGC TCCTCTCATG TGGCAGAGCT GGACTCTAAA 
GCAGAAAGGA TTGCACGCTA CAAGGCTGAA CGCCGCCGTC AGTTAGCAGA GCGCTATGGC 
ATCTCTCTAG ATCAGGAGAT GGAAACAGAC TATACAACTC GCTTCTCCAG ACCCTCCAGA 
GAGCTTGATG GTTCAGACAG ACACCACAGG TCAAAATCAG AAGGGAGAGA TGGAGTCGAG 
CAAAATGCTT ACACCAGCAG CCATCATGTG GATAAGAGCA GCTCAGACTA TCACTCCAGT 
CCAGAGTACA GCCGCGAGCG CACAGACTCC ATCTCAGAAC GTGAAAGAAT GATGAATCTG 
GAGAACCAGA GGAGGGCACA GGAGCGTGAC CGATTACATG GAGGTGGAGG GGTTGCACCT 
GACCCATCTG CGTATATGGA TGTGTCTGGA TCTGCAAGGG TACCAGGAAG GGAGCCAGGG 
ATGATGGGAG TTCCCAGCTC ACCCAATTCA GGCCGAAGGG GCTTGATGCT CTCTCCCAAA 
CAGGGAGCGT CTCCTGGTGA TCTGTTTATT GAGCAGCAGG CTCACAGCAT CCTCCAAAGA 
CACGGTCAGT CATCCAACCA ATGGTCCCTT CAGACCGATT CAGAAGGAGA CACCGTCTCT 
CCAATCAACT GGCCTTCCAG AATCAGAGTT AGAGAGCGAC TGGCCCGCGA TGAGCCATTT 
CACAGGGGTC AAACAACAGA GGAACCTGCT TACAACCAGT CATATCTTCG TTATTACCCT 
CACTCACAGA GCCTCCTAGA TGGTCCTGTC AAACAGCAGC AACTGACCCG TTCTGAAAAA 
AAGGAACGCC ATGATATGGG TTGCCAGAGT TACTTCTCCA TGGGCAGCGA ATCCACTTCC 
AGGAATAAGC AAGAGATTCA GGAGGTGGTT GAAGAAATGG AGGGAAAACA TGTTGCGAGG 
ACTGAAGGTC TTCTGCGGAG CAGAAAGGCA GTTTTACCTT CTGAGATTCG AAGGAAGGAA 
CGGAACACAG ACGATTCATG CTCTGGAAGT GGTGACAACA GGGAGGTAAG CTCAGCTATT 
GCTGGGCACA GGAGCAGCGA GGGTGCTGCA AATGACCCCA GAGGGTGGCA TGGAAGTCGG 
GCAAGACGGG AAGTTTATAC AGAGCACGTG TCTCGACTTC AGGCTGCAGA GTCTTTGCAG 
GCAAGAGCTC GAGAAGCTCA TAGAAGGCCT GATGCTAGCT GCTGCGCCCC AATCCAGACC 
TCCAGTGCAA GACCAATGAC AAATTCATCT AAACCCATGG ATACTGGAAA TACACAAAAA 
ACTGTTCTCC CCATCCAGGA GCCAATCAGA GAGGCTGTCC AACAAGAAAA AGTGCCAAAT 
GTTGATCACC TGTCCCATGA TGCCAGGGTT TCAGTAGCCC ATCTCCGCCA CACATACCTG 
CAAAGTGTTG CAAGTCCCCG GAAAGCCGAA CTGGCAGAAG AGCTGCCTGC AGTTACAGAT 
GTACCCTGGA GAAGCATGCG AGTCAGAGAT CGGTCATCTC GTCGCCCTTT GCGATATATA 
TGTCCCACTG AAAGTAGAAA GACATCTGAG AGGTTTAGAA CACAGCCAAT CACATCAACC 
GAGCGTGAGC AGTCGGACCG GTCTCATCTA AGCTCTGAAC CATCAAGCCC TGAGGTGGAT 
GAAGAGAAAC TAGATGAGAG AGCAAAGCTG AGCGTAGCTG CCAAGCGTTC CCTCTTCAGG 
GAGCTTGAAA AGACTTCAGA TGGTTCCGTT TTAAAGACAT GGAGTCGTAA TCCTGCTGTT 
GAGCGGAGAT TGAGGAGAGG TCAAGATCGA TCCCGCACTC AGCCAGTCAC CACAGAGGAA 
GTAGTTATTG CTGCTACTGC CCCTGGCCAT TTGTCACAGT CCGTGACTTT ACAAACCTCT 
ATAACGCGCG TTCCTAGTCC CACTCTAGTC AGCACCTCTG TGACCAAACA CAGCTTGCAG 
GCTTCGTCTC AGCAAAGCTC CATGGTGAGA GAGCAGGCCA GAGAGGTTCG TCTGGCTCAG 
GAGGCAACGG AGACAGATCG AGCTCGCCAC GCTTCTCCGG GGGAAGGCGG ACACGAGGAG 
CCAGACCTTT CCACACTCAC TCTAGCAGAA AAGATGGCAC TTTTTAACCG TCTTGCCCAG 
CCGTCCAGCC ACGGCTCTAG GGCCAGAGGA GACACGCGTG CACGGAGGAG TAACGCACGC 
TATCAGACTC AACCAATCAC ACTAGGAGAA GTCGAACAGG ATGGTTCTGG TTCTGAGATG 
TTCGATGAGG AATCTGAGTT CTCAATGTGT CTCAGTGATC TGGAACATTT TCAGCCTGCA 
GGTGGAGCTC GTTTTGCACC CAGCTCCAAC TCAGCCATGA GATCCTCCAC TGTTTCCACT 
GAACATGCGG GCGACATCCA TCTGACCATG CCAAATGTAT CTGGGCCTGC ATATTATGAG 
CAGACCGTTT CTCCACCGCA TCACCCTCAG CCTGCCCATG AGATCCAGAC ACATCAGAGT 
CTGGCTGAAG GGGAAAGGCT GCTGTTGAGC ACTGGGAAGA GGAAGCTTCC CTCCCCAGAC 
AGCGTGAAAC GGACACCGCA TGCTCAGCCG GCCAGAGACT GGGACAGTAA GGCTGTGGCG 
GTGACCTCTC AAGACCTTTC ACAAACAGAT GGAGAGCACA CAACAGATCT TCCTGTACAC 
ACTGCCGCTG CTGTATCTGC ATTACAGAGC CATTCTCTCC ATGAGGACCA CACCAGAGAG 
GAAGAGCAGG AGAGCGACTT CAAAACAGAA GACGGAGACG AGCTCTCGGA CGTCATGTCT 
GCCAGACAGA TGTCCATCAA AGAGAGGCTG GCTCTGCTGA AGAAGAGTGG AGAGGAAGAC 
TGGAGGAACA GGATAAATAA AAAACAGGAT GTGGTGAAGG TTGCTGTGTC TGAAAGACAT 
GCCCAGCTAT GGGAGGTGGA GCAGAGCTTC AAGAAGAAGG ATGATGGGAT GATGATAGAT 
GACTTTGCTG TGTCTGATCA ACTCTGGGAT CCTGTCTTTT CCTCCTCCCT TTTGTGTGCC 
TCTGAACCAC TGGATCAAAT AGCAAACCTT CCCAAGGCTA CCCTCCTGCC CGTGCAGGTG 
GATGAGCGTC ACCCCTGGAG ACGGAAGAAG ATGGGAAATG AAGTTGTGGA AAGCCAACAG 
CTTGAGCGAG TAGAGATGTC TATTCAGGAA CGCAAGCAGC TCATCACTTC ACAAGAGGAG 
GCCTGGAAAG CCAAAGGTTA CGGAGCAGCC AACGACTCCA CTCAGTTTAC AGTGGCTGGA 
CGTATGGTCA AAAAAGGCCT GGCAGCCCCT TCTGTTCTCA CAAATCCCGT GTTGTCTCCT 
CTGTCTTCTA AAAACAAGAA TACATCCCAT ACCATCACAA AACCACATGA AGAGATTGAA 
GTCAAACAGA ACATGAAGCT GGAGTCAGAC ATGAAGTTAG AAAAGTTGGA AACATTCCTT 
GGTCGTTTGA ATAGTAAAGT TCCAGGACTT CAGGAGGCCA CAATTGCTGT CACGGAGAAG 
TCTGTAAAAG AGGTCATGAC TCTGGACGAT GAGGTCTTTG ATAAGTTTTA TCGACATGCA 
GAGGATATGG CAAAGTTCAC CAGCAGAGTG GAAATTAGTG ATGATTTTGA TGACATCTTT 
GGAGTACAGC TGCCCAGGTT GACTTCGGAA ATGGTGCAGC ACAAGCGGGC AGTTCGGCCT 
ACCAGAAATG TTCAGTCATC TAAAAACCCG CTGAAAATGC TGGCTGTTCG GGAGGACATC 
AGACATGAGT ACACGGAACA AAGACTGAAC ATCGGCCTTC TGGAGAGCAA GAGGATGAAA 
CAGGAAAAGA TGAATAAGAA CAGCGGTTTC TCTGAGGTTG CTCTCGCCGG ATTGGCCAGT 
AAGGAGAACT TCAGCAGTGT GAGTCTGCGA AGTGTGAATG CCTCTGAACA AAGCTCCAAC 
AACAGTGCCA TGCCCTATAA AAAACTCATG CTCATTCAGG TCAAAGGTCG CAGGCACGTT 
CAGACCAGAC TGGTTGAGCC CAGAGCTTCC TCCTTGAACA GTGGCGACTG CTTCCTCCTG 
ATCACACCAC ATCATTGCTT CATCTGGATT GGAGAATTCG CTAATGTTAT TGAGAAGGCC 
AAAGCTGCAG AGTTGGCCAC GTTCGTTCAA ACCAAGCATG ACCTGGGATG CAGAGCCTCG 
TATGTTCAGA CAATTGAGGA GGGTGCAAAC ACTCACACAC ACGCTGCCAA AGACTTTTGG 
AAGATTCTCG GTGGCCAAAC CAGTTATCAG TCCGCTGGCA CTCCTGAACA AGATGAGTTT 
TATGAGAGTG CAGTCGTAGA AACCAACTGC ATATACAGAC TCATGGAGGA TAAACTTGTT 
CCCCATGATG ACTACTGGGG TCGAGTCCCT CGCTGCTCCA TGCTGAACCC TAAAGAGGTG 
CTGGTATTTG ATTTTGGTAG TGAGGTCTAT GTATGGCATG GCAAGGAGGT GACGTTGGCA 
CAGAGAAAAG TGGCATTTCA GCTGGCGAAG CACTTGTGGA ATGGCACCTT TGACTACACC 
AACTGTGACA TTAATCCTCT GGACCCTGGA GAGTGCAATG CTCTCATACC CAGGAAAGGC 
CAGGGACGAC CAGACTGGGC TGTGTTTGGC AGGTTAACTC AGCACAATGA AACTACTCTT 
TTTAAAGAGA AGTTTTTGGA CTGGAGTGAT TCCAAGAATT CTAAGAAAAA TGGTGACTTG 
CATGTTGAAA ACAAGGAGCT TCGGAGTGGT GAATGTCGGC CCTATGATGC GTCTCTGATG 
CTATCTGCAT TACGGGCGCC AGTCAGGACC GTTCTGGATG GGGTGAACAT TGGACGAGGA 
TATGGATTAG TGGAAGGTGA AGAGGGACGT AATTTTGAGA TCAGCACATT GTCTGTGGAT 
GTCTGGCATA TTTTAGAGTT TGACTACAGT CGACTACCCA AACAGAGCAT TGGTCAGTTT 
CATGAAGGTG ACACATACGT GGTCAAATGG AAGTACATGA TCAGCAGAGC AGTTGGCAAG 
AGACTGCATT CAGAAAGAAT CATCGGTCCA GGGAAGGAGA AATGTTGTTA TTTCTTCTGG 
CAGGGTCGTA ACTCTACGGT GAATGAAAAG GGAACGTCAG CACTGATGAC TGTTGAGCTG 
GATGAGGAAA GAGGAGCTCA GGTCCAGGTA CAGCAAGGAA AGGAGCCTCC TTGTTTCCTA 
CAGTGCTTCA ATGGAGGAAT GATTGTTCAT GCTGGAAAAA GAGAAGAGGA GGAAGACAAT 
ACACAAAACG ACTGGCGATT GTACTGTGTG AGAGGAGAGA AACCCATAGA GGGCCATCTG 
CTGGAGGCAG TGTGTCACTG CAGCAGCTTG CGATCCCGGA CCTCTATGAT CCTCCTGAAC 
ATCCCCAAAG CCAGCCTATA CCTGTGGCAT GGCTGCAAGG CACAAGTGCA CACACGTGAC 
GTGGGCCGTA CCACTGCAAA CAAAATCAAA GAGCAATGTC CTCTGGAGGC AGGGCTTCAC 
AGCAGCAGTA AAGTGTCCAT CCAGGAGTGT GATGAGGGAG CTGAACCACA GGGCTTCTGG 
GAGGCTTTGG GCAAAAGAGA TCGAAAAGCG TACGACTGCA TGCTGCAGGA TCCAGGAAAG 
TTCAACTTCA CTCCTCGGCT TTTCCAGTTA AGCAGTGCCT CGGGAGAGTT TGCAGCAGTG 
GAGTTTGTAT ATCCATCCAG AGAACCAAAT TTGGTTAACT CGATGCCTTT CCTCCAGGAG 
GATTTGTATA CAGCTACTCA GCCAGCCCTG TTTTTGGTGG ATAATCATCA TGAAGTTTAC 
CTGTGGCAGG GCTGGTGGCC TCAGGACAGT GAAAGCACAG GCTCAGCTCG CATACGGTGG 
GACTCTGACA GGAAGTGTGC AATGGAAACT GTGCTCCAGT ATAGCAAAGA AAAAAATGAG 
AAAAAGACAC CAAAATCCTA CCTGATCCAT GCAGGACTGG AGCCTCTTAC ATTCACCAAC 
ATGTTTCCCA GCTGGGAACA CAGAGAGGAC ATCGCTGAAA TCACAGAGAA GGAGGCCGAA 
GTATGTCATC AGATCATTCT TGTTGAAGAT GTCTTGAGCC GACTCTGCAA GACCACTTAC 
CCACTGGCTG AACTAAAAGC ACGTCCCTTA CCGGAAGGTG TCGACCCTCT ACGTCTTGAA 
ACCTACTTAT CAGATGAGGA CTTTGAGGGT GTAGGGGCTT CTGGGAAGAA AGCTTTGGAG 
ATGACGAGAA GTGAATATGA GGCACTGCCT GGATGGAAGC AAGTGAATGT GAAGAAAGCC 
AAAGGATTGT TTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021335686.1
CDS	344..7138
Translation	MYRNPWISNYINHVKLCQGFTRETAPKVGNTKQRRALSFQKEFSFEEKGEGANKEVDVSLLGNTPKVSELRKRFESISTSQSWDMNRKERIARRLEGIDNDVLQSLPGCGGLVSNRLLEEDTPRYTRAADTCDPCTVSLQHYGKSEPSSQSTDVPSRSYAVHPESVFSTGSSHVAELDSKAERIARYKAERRRQLAERYGISLDQEMETDYTTRFSRPSRELDGSDRHHRSKSEGRDGVEQNAYTSSHHVDKSSSDYHSSPEYSRERTDSISERERMMNLENQRRAQERDRLHGGGGVAPDPSAYMDVSGSARVPGREPGMMGVPSSPNSGRRGLMLSPKQGASPGDLFIEQQAHSILQRHGQSSNQWSLQTDSEGDTVSPINWPSRIRVRERLARDEPFHRGQTTEEPAYNQSYLRYYPHSQSLLDGPVKQQQLTRSEKKERHDMGCQSYFSMGSESTSRNKQEIQEVVEEMEGKHVARTEGLLRSRKAVLPSEIRRKERNTDDSCSGSGDNREVSSAIAGHRSSEGAANDPRGWHGSRARREVYTEHVSRLQAAESLQARAREAHRRPDASCCAPIQTSSARPMTNSSKPMDTGNTQKTVLPIQEPIREAVQQEKVPNVDHLSHDARVSVAHLRHTYLQSVASPRKAELAEELPAVTDVPWRSMRVRDRSSRRPLRYICPTESRKTSERFRTQPITSTEREQSDRSHLSSEPSSPEVDEEKLDERAKLSVAAKRSLFRELEKTSDGSVLKTWSRNPAVERRLRRGQDRSRTQPVTTEEVVIAATAPGHLSQSVTLQTSITRVPSPTLVSTSVTKHSLQASSQQSSMVREQAREVRLAQEATETDRARHASPGEGGHEEPDLSTLTLAEKMALFNRLAQPSSHGSRARGDTRARRSNARYQTQPITLGEVEQDGSGSEMFDEESEFSMCLSDLEHFQPAGGARFAPSSNSAMRSSTVSTEHAGDIHLTMPNVSGPAYYEQTVSPPHHPQPAHEIQTHQSLAEGERLLLSTGKRKLPSPDSVKRTPHAQPARDWDSKAVAVTSQDLSQTDGEHTTDLPVHTAAAVSALQSHSLHEDHTREEEQESDFKTEDGDELSDVMSARQMSIKERLALLKKSGEEDWRNRINKKQDVVKVAVSERHAQLWEVEQSFKKKDDGMMIDDFAVSDQLWDPVFSSSLLCASEPLDQIANLPKATLLPVQVDERHPWRRKKMGNEVVESQQLERVEMSIQERKQLITSQEEAWKAKGYGAANDSTQFTVAGRMVKKGLAAPSVLTNPVLSPLSSKNKNTSHTITKPHEEIEVKQNMKLESDMKLEKLETFLGRLNSKVPGLQEATIAVTEKSVKEVMTLDDEVFDKFYRHAEDMAKFTSRVEISDDFDDIFGVQLPRLTSEMVQHKRAVRPTRNVQSSKNPLKMLAVREDIRHEYTEQRLNIGLLESKRMKQEKMNKNSGFSEVALAGLASKENFSSVSLRSVNASEQSSNNSAMPYKKLMLIQVKGRRHVQTRLVEPRASSLNSGDCFLLITPHHCFIWIGEFANVIEKAKAAELATFVQTKHDLGCRASYVQTIEEGANTHTHAAKDFWKILGGQTSYQSAGTPEQDEFYESAVVETNCIYRLMEDKLVPHDDYWGRVPRCSMLNPKEVLVFDFGSEVYVWHGKEVTLAQRKVAFQLAKHLWNGTFDYTNCDINPLDPGECNALIPRKGQGRPDWAVFGRLTQHNETTLFKEKFLDWSDSKNSKKNGDLHVENKELRSGECRPYDASLMLSALRAPVRTVLDGVNIGRGYGLVEGEEGRNFEISTLSVDVWHILEFDYSRLPKQSIGQFHEGDTYVVKWKYMISRAVGKRLHSERIIGPGKEKCCYFFWQGRNSTVNEKGTSALMTVELDEERGAQVQVQQGKEPPCFLQCFNGGMIVHAGKREEEEDNTQNDWRLYCVRGEKPIEGHLLEAVCHCSSLRSRTSMILLNIPKASLYLWHGCKAQVHTRDVGRTTANKIKEQCPLEAGLHSSSKVSIQECDEGAEPQGFWEALGKRDRKAYDCMLQDPGKFNFTPRLFQLSSASGEFAAVEFVYPSREPNLVNSMPFLQEDLYTATQPALFLVDNHHEVYLWQGWWPQDSESTGSARIRWDSDRKCAMETVLQYSKEKNEKKTPKSYLIHAGLEPLTFTNMFPSWEHREDIAEITEKEAEVCHQIILVEDVLSRLCKTTYPLAELKARPLPEGVDPLRLETYLSDEDFEGVGASGKKALEMTRSEYEALPGWKQVNVKKAKGLF

Target ORF information:

RefSeq Version	XM_021480011.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio supervillin a (svila), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021480011.1

ORF Insert Sequence:

ATGTATCGGA ACCCTTGGAT CTCCAACTAT ATCAACCATG TCAAGTTGTG CCAAGGCTTC 
ACCAGAGAAA CAGCCCCTAA AGTTGGCAAT ACCAAGCAAC GGAGAGCTCT TAGTTTTCAG 
AAAGAGTTCT CGTTTGAGGA GAAAGGTGAG GGAGCCAACA AAGAAGTAGA TGTTAGTCTG 
CTGGGGAATA CACCCAAGGT CTCAGAATTA AGGAAACGTT TTGAAAGTAT AAGCACAAGT 
CAGAGTTGGG ACATGAACAG GAAGGAGCGA ATTGCACGGC GCCTGGAAGG AATCGATAAT 
GATGTTCTCC AAAGTCTTCC AGGTTGTGGT GGTCTAGTTT CTAATCGACT GCTGGAGGAG 
GACACTCCAA GATATACCCG TGCAGCAGAC ACCTGTGACC CCTGTACAGT TTCTCTTCAG 
CACTATGGCA AAAGTGAACC AAGTTCACAA AGCACAGATG TTCCATCCAG AAGTTACGCA 
GTCCACCCGG AGTCTGTCTT CAGCACTGGC TCCTCTCATG TGGCAGAGCT GGACTCTAAA 
GCAGAAAGGA TTGCACGCTA CAAGGCTGAA CGCCGCCGTC AGTTAGCAGA GCGCTATGGC 
ATCTCTCTAG ATCAGGAGAT GGAAACAGAC TATACAACTC GCTTCTCCAG ACCCTCCAGA 
GAGCTTGATG GTTCAGACAG ACACCACAGG TCAAAATCAG AAGGGAGAGA TGGAGTCGAG 
CAAAATGCTT ACACCAGCAG CCATCATGTG GATAAGAGCA GCTCAGACTA TCACTCCAGT 
CCAGAGTACA GCCGCGAGCG CACAGACTCC ATCTCAGAAC GTGAAAGAAT GATGAATCTG 
GAGAACCAGA GGAGGGCACA GGAGCGTGAC CGATTACATG GAGGTGGAGG GGTTGCACCT 
GACCCATCTG CGTATATGGA TGTGTCTGGA TCTGCAAGGG TACCAGGAAG GGAGCCAGGG 
ATGATGGGAG TTCCCAGCTC ACCCAATTCA GGCCGAAGGG GCTTGATGCT CTCTCCCAAA 
CAGGGAGCGT CTCCTGGTGA TCTGTTTATT GAGCAGCAGG CTCACAGCAT CCTCCAAAGA 
CACGGTCAGT CATCCAACCA ATGGTCCCTT CAGACCGATT CAGAAGGAGA CACCGTCTCT 
CCAATCAACT GGCCTTCCAG AATCAGAGTT AGAGAGCGAC TGGCCCGCGA TGAGCCATTT 
CACAGGGGTC AAACAACAGA GGAACCTGCT TACAACCAGT CATATCTTCG TTATTACCCT 
CACTCACAGA GCCTCCTAGA TGGTCCTGTC AAACAGCAGC AACTGACCCG TTCTGAAAAA 
AAGGAACGCC ATGATATGGG TTGCCAGAGT TACTTCTCCA TGGGCAGCGA ATCCACTTCC 
AGGAATAAGC AAGAGATTCA GGAGGTGGTT GAAGAAATGG AGGGAAAACA TGTTGCGAGG 
ACTGAAGGTC TTCTGCGGAG CAGAAAGGCA GTTTTACCTT CTGAGATTCG AAGGAAGGAA 
CGGAACACAG ACGATTCATG CTCTGGAAGT GGTGACAACA GGGAGGTAAG CTCAGCTATT 
GCTGGGCACA GGAGCAGCGA GGGTGCTGCA AATGACCCCA GAGGGTGGCA TGGAAGTCGG 
GCAAGACGGG AAGTTTATAC AGAGCACGTG TCTCGACTTC AGGCTGCAGA GTCTTTGCAG 
GCAAGAGCTC GAGAAGCTCA TAGAAGGCCT GATGCTAGCT GCTGCGCCCC AATCCAGACC 
TCCAGTGCAA GACCAATGAC AAATTCATCT AAACCCATGG ATACTGGAAA TACACAAAAA 
ACTGTTCTCC CCATCCAGGA GCCAATCAGA GAGGCTGTCC AACAAGAAAA AGTGCCAAAT 
GTTGATCACC TGTCCCATGA TGCCAGGGTT TCAGTAGCCC ATCTCCGCCA CACATACCTG 
CAAAGTGTTG CAAGTCCCCG GAAAGCCGAA CTGGCAGAAG AGCTGCCTGC AGTTACAGAT 
GTACCCTGGA GAAGCATGCG AGTCAGAGAT CGGTCATCTC GTCGCCCTTT GCGATATATA 
TGTCCCACTG AAAGTAGAAA GACATCTGAG AGGTTTAGAA CACAGCCAAT CACATCAACC 
GAGCGTGAGC AGTCGGACCG GTCTCATCTA AGCTCTGAAC CATCAAGCCC TGAGGTGGAT 
GAAGAGAAAC TAGATGAGAG AGCAAAGCTG AGCGTAGCTG CCAAGCGTTC CCTCTTCAGG 
GAGCTTGAAA AGACTTCAGA TGGTTCCGTT TTAAAGACAT GGAGTCGTAA TCCTGCTGTT 
GAGCGGAGAT TGAGGAGAGG TCAAGATCGA TCCCGCACTC AGCCAGTCAC CACAGAGGAA 
GTAGTTATTG CTGCTACTGC CCCTGGCCAT TTGTCACAGT CCGTGACTTT ACAAACCTCT 
ATAACGCGCG TTCCTAGTCC CACTCTAGTC AGCACCTCTG TGACCAAACA CAGCTTGCAG 
GCTTCGTCTC AGCAAAGCTC CATGGTGAGA GAGCAGGCCA GAGAGGTTCG TCTGGCTCAG 
GAGGCAACGG AGACAGATCG AGCTCGCCAC GCTTCTCCGG GGGAAGGCGG ACACGAGGAG 
CCAGACCTTT CCACACTCAC TCTAGCAGAA AAGATGGCAC TTTTTAACCG TCTTGCCCAG 
CCGTCCAGCC ACGGCTCTAG GGCCAGAGGA GACACGCGTG CACGGAGGAG TAACGCACGC 
TATCAGACTC AACCAATCAC ACTAGGAGAA GTCGAACAGG ATGGTTCTGG TTCTGAGATG 
TTCGATGAGG AATCTGAGTT CTCAATGTGT CTCAGTGATC TGGAACATTT TCAGCCTGCA 
GGTGGAGCTC GTTTTGCACC CAGCTCCAAC TCAGCCATGA GATCCTCCAC TGTTTCCACT 
GAACATGCGG GCGACATCCA TCTGACCATG CCAAATGTAT CTGGGCCTGC ATATTATGAG 
CAGACCGTTT CTCCACCGCA TCACCCTCAG CCTGCCCATG AGATCCAGAC ACATCAGAGT 
CTGGCTGAAG GGGAAAGGCT GCTGTTGAGC ACTGGGAAGA GGAAGCTTCC CTCCCCAGAC 
AGCGTGAAAC GGACACCGCA TGCTCAGCCG GCCAGAGACT GGGACAGTAA GGCTGTGGCG 
GTGACCTCTC AAGACCTTTC ACAAACAGAT GGAGAGCACA CAACAGATCT TCCTGTACAC 
ACTGCCGCTG CTGTATCTGC ATTACAGAGC CATTCTCTCC ATGAGGACCA CACCAGAGAG 
GAAGAGCAGG AGAGCGACTT CAAAACAGAA GACGGAGACG AGCTCTCGGA CGTCATGTCT 
GCCAGACAGA TGTCCATCAA AGAGAGGCTG GCTCTGCTGA AGAAGAGTGG AGAGGAAGAC 
TGGAGGAACA GGATAAATAA AAAACAGGAT GTGGTGAAGG TTGCTGTGTC TGAAAGACAT 
GCCCAGCTAT GGGAGGTGGA GCAGAGCTTC AAGAAGAAGG ATGATGGGAT GATGATAGAT 
GACTTTGCTG TGTCTGATCA ACTCTGGGAT CCTGTCTTTT CCTCCTCCCT TTTGTGTGCC 
TCTGAACCAC TGGATCAAAT AGCAAACCTT CCCAAGGCTA CCCTCCTGCC CGTGCAGGTG 
GATGAGCGTC ACCCCTGGAG ACGGAAGAAG ATGGGAAATG AAGTTGTGGA AAGCCAACAG 
CTTGAGCGAG TAGAGATGTC TATTCAGGAA CGCAAGCAGC TCATCACTTC ACAAGAGGAG 
GCCTGGAAAG CCAAAGGTTA CGGAGCAGCC AACGACTCCA CTCAGTTTAC AGTGGCTGGA 
CGTATGGTCA AAAAAGGCCT GGCAGCCCCT TCTGTTCTCA CAAATCCCGT GTTGTCTCCT 
CTGTCTTCTA AAAACAAGAA TACATCCCAT ACCATCACAA AACCACATGA AGAGATTGAA 
GTCAAACAGA ACATGAAGCT GGAGTCAGAC ATGAAGTTAG AAAAGTTGGA AACATTCCTT 
GGTCGTTTGA ATAGTAAAGT TCCAGGACTT CAGGAGGCCA CAATTGCTGT CACGGAGAAG 
TCTGTAAAAG AGGTCATGAC TCTGGACGAT GAGGTCTTTG ATAAGTTTTA TCGACATGCA 
GAGGATATGG CAAAGTTCAC CAGCAGAGTG GAAATTAGTG ATGATTTTGA TGACATCTTT 
GGAGTACAGC TGCCCAGGTT GACTTCGGAA ATGGTGCAGC ACAAGCGGGC AGTTCGGCCT 
ACCAGAAATG TTCAGTCATC TAAAAACCCG CTGAAAATGC TGGCTGTTCG GGAGGACATC 
AGACATGAGT ACACGGAACA AAGACTGAAC ATCGGCCTTC TGGAGAGCAA GAGGATGAAA 
CAGGAAAAGA TGAATAAGAA CAGCGGTTTC TCTGAGGTTG CTCTCGCCGG ATTGGCCAGT 
AAGGAGAACT TCAGCAGTGT GAGTCTGCGA AGTGTGAATG CCTCTGAACA AAGCTCCAAC 
AACAGTGCCA TGCCCTATAA AAAACTCATG CTCATTCAGG TCAAAGGTCG CAGGCACGTT 
CAGACCAGAC TGGTTGAGCC CAGAGCTTCC TCCTTGAACA GTGGCGACTG CTTCCTCCTG 
ATCACACCAC ATCATTGCTT CATCTGGATT GGAGAATTCG CTAATGTTAT TGAGAAGGCC 
AAAGCTGCAG AGTTGGCCAC GTTCGTTCAA ACCAAGCATG ACCTGGGATG CAGAGCCTCG 
TATGTTCAGA CAATTGAGGA GGGTGCAAAC ACTCACACAC ACGCTGCCAA AGACTTTTGG 
AAGATTCTCG GTGGCCAAAC CAGTTATCAG TCCGCTGGCA CTCCTGAACA AGATGAGTTT 
TATGAGAGTG CAGTCGTAGA AACCAACTGC ATATACAGAC TCATGGAGGA TAAACTTGTT 
CCCCATGATG ACTACTGGGG TCGAGTCCCT CGCTGCTCCA TGCTGAACCC TAAAGAGGTG 
CTGGTATTTG ATTTTGGTAG TGAGGTCTAT GTATGGCATG GCAAGGAGGT GACGTTGGCA 
CAGAGAAAAG TGGCATTTCA GCTGGCGAAG CACTTGTGGA ATGGCACCTT TGACTACACC 
AACTGTGACA TTAATCCTCT GGACCCTGGA GAGTGCAATG CTCTCATACC CAGGAAAGGC 
CAGGGACGAC CAGACTGGGC TGTGTTTGGC AGGTTAACTC AGCACAATGA AACTACTCTT 
TTTAAAGAGA AGTTTTTGGA CTGGAGTGAT TCCAAGAATT CTAAGAAAAA TGGTGACTTG 
CATGTTGAAA ACAAGGAGCT TCGGAGTGGT GAATGTCGGC CCTATGATGC GTCTCTGATG 
CTATCTGCAT TACGGGCGCC AGTCAGGACC GTTCTGGATG GGGTGAACAT TGGACGAGGA 
TATGGATTAG TGGAAGGTGA AGAGGGACGT AATTTTGAGA TCAGCACATT GTCTGTGGAT 
GTCTGGCATA TTTTAGAGTT TGACTACAGT CGACTACCCA AACAGAGCAT TGGTCAGTTT 
CATGAAGGTG ACACATACGT GGTCAAATGG AAGTACATGA TCAGCAGAGC AGTTGGCAAG 
AGACTGCATT CAGAAAGAAT CATCGGTCCA GGGAAGGAGA AATGTTGTTA TTTCTTCTGG 
CAGGGTCGTA ACTCTACGGT GAATGAAAAG GGAACGTCAG CACTGATGAC TGTTGAGCTG 
GATGAGGAAA GAGGAGCTCA GGTCCAGGTA CAGCAAGGAA AGGAGCCTCC TTGTTTCCTA 
CAGTGCTTCA ATGGAGGAAT GATTGTTCAT GCTGGAAAAA GAGAAGAGGA GGAAGACAAT 
ACACAAAACG ACTGGCGATT GTACTGTGTG AGAGGAGAGA AACCCATAGA GGGCCATCTG 
CTGGAGGCAG TGTGTCACTG CAGCAGCTTG CGATCCCGGA CCTCTATGAT CCTCCTGAAC 
ATCCCCAAAG CCAGCCTATA CCTGTGGCAT GGCTGCAAGG CACAAGTGCA CACACGTGAC 
GTGGGCCGTA CCACTGCAAA CAAAATCAAA GAGCAATGTC CTCTGGAGGC AGGGCTTCAC 
AGCAGCAGTA AAGTGTCCAT CCAGGAGTGT GATGAGGGAG CTGAACCACA GGGCTTCTGG 
GAGGCTTTGG GCAAAAGAGA TCGAAAAGCG TACGACTGCA TGCTGCAGGA TCCAGGAAAG 
TTCAACTTCA CTCCTCGGCT TTTCCAGTTA AGCAGTGCCT CGGGAGAGTT TGCAGCAGTG 
GAGTTTGTAT ATCCATCCAG AGAACCAAAT TTGGTTAACT CGATGCCTTT CCTCCAGGAG 
GATTTGTATA CAGCTACTCA GCCAGCCCTG TTTTTGGTGG ATAATCATCA TGAAGTTTAC 
CTGTGGCAGG GCTGGTGGCC TCAGGACAGT GAAAGCACAG GCTCAGCTCG CATACGGTGG 
GACTCTGACA GGAAGTGTGC AATGGAAACT GTGCTCCAGT ATAGCAAAGA AAAAAATGAG 
AAAAAGACAC CAAAATCCTA CCTGATCCAT GCAGGACTGG AGCCTCTTAC ATTCACCAAC 
ATGTTTCCCA GCTGGGAACA CAGAGAGGAC ATCGCTGAAA TCACAGAGAA GGAGGCCGAA 
GTATGTCATC AGATCATTCT TGTTGAAGAT GTCTTGAGCC GACTCTGCAA GACCACTTAC 
CCACTGGCTG AACTAAAAGC ACGTCCCTTA CCGGAAGGTG TCGACCCTCT ACGTCTTGAA 
ACCTACTTAT CAGATGAGGA CTTTGAGGGT GTAGGGGCTT CTGGGAAGAA AGCTTTGGAG 
ATGACGAGAA GTGAATATGA GGCACTGCCT GGATGGAAGC AAGTGAATGT GAAGAAAGCC 
AAAGGATTGT TTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

svila ( XM_021480012.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021335687.1 Danio rerio supervillin a (svila), transcript variant X2, mRNA.

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CloneID	ODa148130
Clone ID Related Accession (Same CDS sequence)	XM_021480012.1
Accession Version	XM_021480012.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6726bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	supervillin isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007123.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTATCGGA ACCCTTGGAT CTCCAACTAT ATCAACCATG TCAAGTTGTG CCAAGGCTTC 
ACCAGAGAAA CAGCCCCTAA AGTTGGCAAT ACCAAGCAAC GGAGAGCTCT TAGTTTTCAG 
AAAGAGTTCT CGTTTGAGGA GAAAGGTGAG GGAGCCAACA AAGAAGTAGA TGTTAGTCTG 
CTGGGGAATA CACCCAAGGT CTCAGAATTA AGGAAACGTT TTGAAAGTAT AAGCACAAGT 
CAGAGTTGGG ACATGAACAG GAAGGAGCGA ATTGCACGGC GCCTGGAAGG AATCGATAAT 
GATGTTCTCC AAAGTCTTCC AGGTTGTGGT GGTCTAGTTT CTAATCGACT GCTGGAGGAG 
GACACTCCAA GATATACCCG TGCAGCAGAC ACCTGTGACC CCTGTACAGT TTCTCTTCAG 
CACTATGGCA AAAGTGAACC AAGTTCACAA AGCACAGATG TTCCATCCAG AAGTTACGCA 
GTCCACCCGG AGTCTGTCTT CAGCACTGGC TCCTCTCATG TGGCAGAGCT GGACTCTAAA 
GCAGAAAGGA TTGCACGCTA CAAGGCTGAA CGCCGCCGTC AGTTAGCAGA GCGCTATGGC 
ATCTCTCTAG ATCAGGAGAT GGAAACAGAC TATACAACTC GCTTCTCCAG ACCCTCCAGA 
GAGCTTGATG GTTCAGACAG ACACCACAGG TCAAAATCAG AAGGGAGAGA TGGAGTCGAG 
CAAAATGCTT ACACCAGCAG CCATCATGTG GATAAGAGCA GCTCAGACTA TCACTCCAGT 
CCAGAGTACA GCCGCGAGCG CACAGACTCC ATCTCAGAAC GTGAAAGAAT GATGAATCTG 
GAGAACCAGA GGAGGGCACA GGAGCGTGAC CGATTACATG GAGGTGGAGG GGTTGCACCT 
GACCCATCTG CGTATATGGA TGTGTCTGGA TCTGCAAGGG TACCAGGAAG GGAGCCAGGG 
ATGATGGGAG TTCCCAGCTC ACCCAATTCA GGCCGAAGGG GCTTGATGCT CTCTCCCAAA 
CAGGGAGCGT CTCCTGGTGA TCTGTTTATT GAGCAGCAGG CTCACAGCAT CCTCCAAAGA 
CACGGTCAGT CATCCAACCA ATGGTCCCTT CAGACCGATT CAGAAGGAGA CACCGTCTCT 
CCAATCAACT GGCCTTCCAG AATCAGAGTT AGAGAGCGAC TGGCCCGCGA TGAGCCATTT 
CACAGGGGTC AAACAACAGA GGAACCTGCT TACAACCAGT CATATCTTCG TTATTACCCT 
CACTCACAGA GCCTCCTAGA TGGTCCTGTC AAACAGCAGC AACTGACCCG TTCTGAAAAA 
AAGGAACGCC ATGATATGGG TTGCCAGAGT TACTTCTCCA TGGGCAGCGA ATCCACTTCC 
AGGAATAAGC AAGAGATTCA GGAGGTGGTT GAAGAAATGG AGGGAAAACA TGTTGCGAGG 
ACTGAAGGTC TTCTGCGGAG CAGAAAGGCA GTTTTACCTT CTGAGATTCG AAGGAAGGAA 
CGGAACACAG ACGATTCATG CTCTGGAAGT GGTGACAACA GGGAGGTAAG CTCAGCTATT 
GCTGGGCACA GGAGCAGCGA GGGTGCTGCA AATGACCCCA GAGGGTGGCA TGGAAGTCGG 
GCAAGACGGG AAGTTTATAC AGAGCACGTG TCTCGACTTC AGGCTGCAGA GTCTTTGCAG 
GCAAGAGCTC GAGAAGCTCA TAGAAGGCCT GATGCTAGCT GCTGCGCCCC AATCCAGACC 
TCCAGTGCAA GACCAATGAC AAATTCATCT AAACCCATGG ATACTGGAAA TACACAAAAA 
ACTGTTCTCC CCATCCAGGA GCCAATCAGA GAGGCTGTCC AACAAGAAAA AGTGCCAAAT 
GTTGATCACC TGTCCCATGA TGCCAGGGTT TCAGTAGCCC ATCTCCGCCA CACATACCTG 
CAAAGTGTTG CAAGTCCCCG GAAAGCCGAA CTGGCAGAAG AGCTGCCTGC AGTTACAGAT 
GTACCCTGGA GAAGCATGCG AGTCAGAGAT CGGTCATCTC GTCGCCCTTT GCGATATATA 
TGTCCCACTG AAAGTAGAAA GACATCTGAG AGGTTTAGAA CACAGCCAAT CACATCAACC 
GAGCGTGAGC AGTCGGACCG GTCTCATCTA AGCTCTGAAC CATCAAGCCC TGAGGTGGAT 
GAAGAGAAAC TAGATGAGAG AGCAAAGCTG AGCGTAGCTG CCAAGCGTTC CCTCTTCAGG 
GAGCTTGAAA AGACTTCAGA TGGTTCCGTT TTAAAGACAT GGAGTCGTAA TCCTGCTGTT 
GAGCGGAGAT TGAGGAGAGG TCAAGATCGA TCCCGCACTC AGCCAGTCAC CACAGAGGAA 
GTAGTTATTG CTGCTACTGC CCCTGGCCAT TTGTCACAGT CCGTGACTTT ACAAACCTCT 
ATAACGCGCG TTCCTAGTCC CACTCTAGTC AGCACCTCTG TGACCAAACA CAGCTTGCAG 
GCTTCGTCTC AGCAAAGCTC CATGGTGAGA GAGCAGGCCA GAGAGGTTCG TCTGGCTCAG 
GAGGCAACGG AGACAGATCG AGCTCGCCAC GCTTCTCCGG GGGAAGGCGG ACACGAGGAG 
CCAGACCTTT CCACACTCAC TCTAGCAGAA AAGATGGCAC TTTTTAACCG TCTTGCCCAG 
CCGTCCAGCC ACGGCTCTAG GGCCAGAGGA GACACGCGTG CACGGAGGAG TAACGCACGC 
TATCAGACTC AACCAATCAC ACTAGGAGAA GTCGAACAGT TTCAGCCTGC AGGTGGAGCT 
CGTTTTGCAC CCAGCTCCAA CTCAGCCATG AGATCCTCCA CTGTTTCCAC TGAACATGCG 
GGCGACATCC ATCTGACCAT GCCAAATGTA TCTGGGCCTG CATATTATGA GCAGACCGTT 
TCTCCACCGC ATCACCCTCA GCCTGCCCAT GAGATCCAGA CACATCAGAG TCTGGCTGAA 
GGGGAAAGGC TGCTGTTGAG CACTGGGAAG AGGAAGCTTC CCTCCCCAGA CAGCGTGAAA 
CGGACACCGC ATGCTCAGCC GGCCAGAGAC TGGGACAGTA AGGCTGTGGC GGTGACCTCT 
CAAGACCTTT CACAAACAGA TGGAGAGCAC ACAACAGATC TTCCTGTACA CACTGCCGCT 
GCTGTATCTG CATTACAGAG CCATTCTCTC CATGAGGACC ACACCAGAGA GGAAGAGCAG 
GAGAGCGACT TCAAAACAGA AGACGGAGAC GAGCTCTCGG ACGTCATGTC TGCCAGACAG 
ATGTCCATCA AAGAGAGGCT GGCTCTGCTG AAGAAGAGTG GAGAGGAAGA CTGGAGGAAC 
AGGATAAATA AAAAACAGGA TGTGGTGAAG GTTGCTGTGT CTGAAAGACA TGCCCAGCTA 
TGGGAGGTGG AGCAGAGCTT CAAGAAGAAG GATGATGGGA TGATGATAGA TGACTTTGCT 
GTGTCTGATC AACTCTGGGA TCCTGTCTTT TCCTCCTCCC TTTTGTGTGC CTCTGAACCA 
CTGGATCAAA TAGCAAACCT TCCCAAGGCT ACCCTCCTGC CCGTGCAGGT GGATGAGCGT 
CACCCCTGGA GACGGAAGAA GATGGGAAAT GAAGTTGTGG AAAGCCAACA GCTTGAGCGA 
GTAGAGATGT CTATTCAGGA ACGCAAGCAG CTCATCACTT CACAAGAGGA GGCCTGGAAA 
GCCAAAGGTT ACGGAGCAGC CAACGACTCC ACTCAGTTTA CAGTGGCTGG ACGTATGGTC 
AAAAAAGGCC TGGCAGCCCC TTCTGTTCTC ACAAATCCCG TGTTGTCTCC TCTGTCTTCT 
AAAAACAAGA ATACATCCCA TACCATCACA AAACCACATG AAGAGATTGA AGTCAAACAG 
AACATGAAGC TGGAGTCAGA CATGAAGTTA GAAAAGTTGG AAACATTCCT TGGTCGTTTG 
AATAGTAAAG TTCCAGGACT TCAGGAGGCC ACAATTGCTG TCACGGAGAA GTCTGTAAAA 
GAGGTCATGA CTCTGGACGA TGAGGTCTTT GATAAGTTTT ATCGACATGC AGAGGATATG 
GCAAAGTTCA CCAGCAGAGT GGAAATTAGT GATGATTTTG ATGACATCTT TGGAGTACAG 
CTGCCCAGGT TGACTTCGGA AATGGTGCAG CACAAGCGGG CAGTTCGGCC TACCAGAAAT 
GTTCAGTCAT CTAAAAACCC GCTGAAAATG CTGGCTGTTC GGGAGGACAT CAGACATGAG 
TACACGGAAC AAAGACTGAA CATCGGCCTT CTGGAGAGCA AGAGGATGAA ACAGGAAAAG 
ATGAATAAGA ACAGCGGTTT CTCTGAGGTT GCTCTCGCCG GATTGGCCAG TAAGGAGAAC 
TTCAGCAGTG TGAGTCTGCG AAGTGTGAAT GCCTCTGAAC AAAGCTCCAA CAACAGTGCC 
ATGCCCTATA AAAAACTCAT GCTCATTCAG GTCAAAGGTC GCAGGCACGT TCAGACCAGA 
CTGGTTGAGC CCAGAGCTTC CTCCTTGAAC AGTGGCGACT GCTTCCTCCT GATCACACCA 
CATCATTGCT TCATCTGGAT TGGAGAATTC GCTAATGTTA TTGAGAAGGC CAAAGCTGCA 
GAGTTGGCCA CGTTCGTTCA AACCAAGCAT GACCTGGGAT GCAGAGCCTC GTATGTTCAG 
ACAATTGAGG AGGGTGCAAA CACTCACACA CACGCTGCCA AAGACTTTTG GAAGATTCTC 
GGTGGCCAAA CCAGTTATCA GTCCGCTGGC ACTCCTGAAC AAGATGAGTT TTATGAGAGT 
GCAGTCGTAG AAACCAACTG CATATACAGA CTCATGGAGG ATAAACTTGT TCCCCATGAT 
GACTACTGGG GTCGAGTCCC TCGCTGCTCC ATGCTGAACC CTAAAGAGGT GCTGGTATTT 
GATTTTGGTA GTGAGGTCTA TGTATGGCAT GGCAAGGAGG TGACGTTGGC ACAGAGAAAA 
GTGGCATTTC AGCTGGCGAA GCACTTGTGG AATGGCACCT TTGACTACAC CAACTGTGAC 
ATTAATCCTC TGGACCCTGG AGAGTGCAAT GCTCTCATAC CCAGGAAAGG CCAGGGACGA 
CCAGACTGGG CTGTGTTTGG CAGGTTAACT CAGCACAATG AAACTACTCT TTTTAAAGAG 
AAGTTTTTGG ACTGGAGTGA TTCCAAGAAT TCTAAGAAAA ATGGTGACTT GCATGTTGAA 
AACAAGGAGC TTCGGAGTGG TGAATGTCGG CCCTATGATG CGTCTCTGAT GCTATCTGCA 
TTACGGGCGC CAGTCAGGAC CGTTCTGGAT GGGGTGAACA TTGGACGAGG ATATGGATTA 
GTGGAAGGTG AAGAGGGACG TAATTTTGAG ATCAGCACAT TGTCTGTGGA TGTCTGGCAT 
ATTTTAGAGT TTGACTACAG TCGACTACCC AAACAGAGCA TTGGTCAGTT TCATGAAGGT 
GACACATACG TGGTCAAATG GAAGTACATG ATCAGCAGAG CAGTTGGCAA GAGACTGCAT 
TCAGAAAGAA TCATCGGTCC AGGGAAGGAG AAATGTTGTT ATTTCTTCTG GCAGGGTCGT 
AACTCTACGG TGAATGAAAA GGGAACGTCA GCACTGATGA CTGTTGAGCT GGATGAGGAA 
AGAGGAGCTC AGGTCCAGGT ACAGCAAGGA AAGGAGCCTC CTTGTTTCCT ACAGTGCTTC 
AATGGAGGAA TGATTGTTCA TGCTGGAAAA AGAGAAGAGG AGGAAGACAA TACACAAAAC 
GACTGGCGAT TGTACTGTGT GAGAGGAGAG AAACCCATAG AGGGCCATCT GCTGGAGGCA 
GTGTGTCACT GCAGCAGCTT GCGATCCCGG ACCTCTATGA TCCTCCTGAA CATCCCCAAA 
GCCAGCCTAT ACCTGTGGCA TGGCTGCAAG GCACAAGTGC ACACACGTGA CGTGGGCCGT 
ACCACTGCAA ACAAAATCAA AGAGCAATGT CCTCTGGAGG CAGGGCTTCA CAGCAGCAGT 
AAAGTGTCCA TCCAGGAGTG TGATGAGGGA GCTGAACCAC AGGGCTTCTG GGAGGCTTTG 
GGCAAAAGAG ATCGAAAAGC GTACGACTGC ATGCTGCAGG ATCCAGGAAA GTTCAACTTC 
ACTCCTCGGC TTTTCCAGTT AAGCAGTGCC TCGGGAGAGT TTGCAGCAGT GGAGTTTGTA 
TATCCATCCA GAGAACCAAA TTTGGTTAAC TCGATGCCTT TCCTCCAGGA GGATTTGTAT 
ACAGCTACTC AGCCAGCCCT GTTTTTGGTG GATAATCATC ATGAAGTTTA CCTGTGGCAG 
GGCTGGTGGC CTCAGGACAG TGAAAGCACA GGCTCAGCTC GCATACGGTG GGACTCTGAC 
AGGAAGTGTG CAATGGAAAC TGTGCTCCAG TATAGCAAAG AAAAAAATGA GAAAAAGACA 
CCAAAATCCT ACCTGATCCA TGCAGGACTG GAGCCTCTTA CATTCACCAA CATGTTTCCC 
AGCTGGGAAC ACAGAGAGGA CATCGCTGAA ATCACAGAGA AGGAGGCCGA AGTATGTCAT 
CAGATCATTC TTGTTGAAGA TGTCTTGAGC CGACTCTGCA AGACCACTTA CCCACTGGCT 
GAACTAAAAG CACGTCCCTT ACCGGAAGGT GTCGACCCTC TACGTCTTGA AACCTACTTA 
TCAGATGAGG ACTTTGAGGG TGTAGGGGCT TCTGGGAAGA AAGCTTTGGA GATGACGAGA 
AGTGAATATG AGGCACTGCC TGGATGGAAG CAAGTGAATG TGAAGAAAGC CAAAGGATTG 
TTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021335687.1
CDS	343..7068
Translation	MYRNPWISNYINHVKLCQGFTRETAPKVGNTKQRRALSFQKEFSFEEKGEGANKEVDVSLLGNTPKVSELRKRFESISTSQSWDMNRKERIARRLEGIDNDVLQSLPGCGGLVSNRLLEEDTPRYTRAADTCDPCTVSLQHYGKSEPSSQSTDVPSRSYAVHPESVFSTGSSHVAELDSKAERIARYKAERRRQLAERYGISLDQEMETDYTTRFSRPSRELDGSDRHHRSKSEGRDGVEQNAYTSSHHVDKSSSDYHSSPEYSRERTDSISERERMMNLENQRRAQERDRLHGGGGVAPDPSAYMDVSGSARVPGREPGMMGVPSSPNSGRRGLMLSPKQGASPGDLFIEQQAHSILQRHGQSSNQWSLQTDSEGDTVSPINWPSRIRVRERLARDEPFHRGQTTEEPAYNQSYLRYYPHSQSLLDGPVKQQQLTRSEKKERHDMGCQSYFSMGSESTSRNKQEIQEVVEEMEGKHVARTEGLLRSRKAVLPSEIRRKERNTDDSCSGSGDNREVSSAIAGHRSSEGAANDPRGWHGSRARREVYTEHVSRLQAAESLQARAREAHRRPDASCCAPIQTSSARPMTNSSKPMDTGNTQKTVLPIQEPIREAVQQEKVPNVDHLSHDARVSVAHLRHTYLQSVASPRKAELAEELPAVTDVPWRSMRVRDRSSRRPLRYICPTESRKTSERFRTQPITSTEREQSDRSHLSSEPSSPEVDEEKLDERAKLSVAAKRSLFRELEKTSDGSVLKTWSRNPAVERRLRRGQDRSRTQPVTTEEVVIAATAPGHLSQSVTLQTSITRVPSPTLVSTSVTKHSLQASSQQSSMVREQAREVRLAQEATETDRARHASPGEGGHEEPDLSTLTLAEKMALFNRLAQPSSHGSRARGDTRARRSNARYQTQPITLGEVEQFQPAGGARFAPSSNSAMRSSTVSTEHAGDIHLTMPNVSGPAYYEQTVSPPHHPQPAHEIQTHQSLAEGERLLLSTGKRKLPSPDSVKRTPHAQPARDWDSKAVAVTSQDLSQTDGEHTTDLPVHTAAAVSALQSHSLHEDHTREEEQESDFKTEDGDELSDVMSARQMSIKERLALLKKSGEEDWRNRINKKQDVVKVAVSERHAQLWEVEQSFKKKDDGMMIDDFAVSDQLWDPVFSSSLLCASEPLDQIANLPKATLLPVQVDERHPWRRKKMGNEVVESQQLERVEMSIQERKQLITSQEEAWKAKGYGAANDSTQFTVAGRMVKKGLAAPSVLTNPVLSPLSSKNKNTSHTITKPHEEIEVKQNMKLESDMKLEKLETFLGRLNSKVPGLQEATIAVTEKSVKEVMTLDDEVFDKFYRHAEDMAKFTSRVEISDDFDDIFGVQLPRLTSEMVQHKRAVRPTRNVQSSKNPLKMLAVREDIRHEYTEQRLNIGLLESKRMKQEKMNKNSGFSEVALAGLASKENFSSVSLRSVNASEQSSNNSAMPYKKLMLIQVKGRRHVQTRLVEPRASSLNSGDCFLLITPHHCFIWIGEFANVIEKAKAAELATFVQTKHDLGCRASYVQTIEEGANTHTHAAKDFWKILGGQTSYQSAGTPEQDEFYESAVVETNCIYRLMEDKLVPHDDYWGRVPRCSMLNPKEVLVFDFGSEVYVWHGKEVTLAQRKVAFQLAKHLWNGTFDYTNCDINPLDPGECNALIPRKGQGRPDWAVFGRLTQHNETTLFKEKFLDWSDSKNSKKNGDLHVENKELRSGECRPYDASLMLSALRAPVRTVLDGVNIGRGYGLVEGEEGRNFEISTLSVDVWHILEFDYSRLPKQSIGQFHEGDTYVVKWKYMISRAVGKRLHSERIIGPGKEKCCYFFWQGRNSTVNEKGTSALMTVELDEERGAQVQVQQGKEPPCFLQCFNGGMIVHAGKREEEEDNTQNDWRLYCVRGEKPIEGHLLEAVCHCSSLRSRTSMILLNIPKASLYLWHGCKAQVHTRDVGRTTANKIKEQCPLEAGLHSSSKVSIQECDEGAEPQGFWEALGKRDRKAYDCMLQDPGKFNFTPRLFQLSSASGEFAAVEFVYPSREPNLVNSMPFLQEDLYTATQPALFLVDNHHEVYLWQGWWPQDSESTGSARIRWDSDRKCAMETVLQYSKEKNEKKTPKSYLIHAGLEPLTFTNMFPSWEHREDIAEITEKEAEVCHQIILVEDVLSRLCKTTYPLAELKARPLPEGVDPLRLETYLSDEDFEGVGASGKKALEMTRSEYEALPGWKQVNVKKAKGLF

Target ORF information:

RefSeq Version	XM_021480012.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio supervillin a (svila), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021480012.1

ORF Insert Sequence:

ATGTATCGGA ACCCTTGGAT CTCCAACTAT ATCAACCATG TCAAGTTGTG CCAAGGCTTC 
ACCAGAGAAA CAGCCCCTAA AGTTGGCAAT ACCAAGCAAC GGAGAGCTCT TAGTTTTCAG 
AAAGAGTTCT CGTTTGAGGA GAAAGGTGAG GGAGCCAACA AAGAAGTAGA TGTTAGTCTG 
CTGGGGAATA CACCCAAGGT CTCAGAATTA AGGAAACGTT TTGAAAGTAT AAGCACAAGT 
CAGAGTTGGG ACATGAACAG GAAGGAGCGA ATTGCACGGC GCCTGGAAGG AATCGATAAT 
GATGTTCTCC AAAGTCTTCC AGGTTGTGGT GGTCTAGTTT CTAATCGACT GCTGGAGGAG 
GACACTCCAA GATATACCCG TGCAGCAGAC ACCTGTGACC CCTGTACAGT TTCTCTTCAG 
CACTATGGCA AAAGTGAACC AAGTTCACAA AGCACAGATG TTCCATCCAG AAGTTACGCA 
GTCCACCCGG AGTCTGTCTT CAGCACTGGC TCCTCTCATG TGGCAGAGCT GGACTCTAAA 
GCAGAAAGGA TTGCACGCTA CAAGGCTGAA CGCCGCCGTC AGTTAGCAGA GCGCTATGGC 
ATCTCTCTAG ATCAGGAGAT GGAAACAGAC TATACAACTC GCTTCTCCAG ACCCTCCAGA 
GAGCTTGATG GTTCAGACAG ACACCACAGG TCAAAATCAG AAGGGAGAGA TGGAGTCGAG 
CAAAATGCTT ACACCAGCAG CCATCATGTG GATAAGAGCA GCTCAGACTA TCACTCCAGT 
CCAGAGTACA GCCGCGAGCG CACAGACTCC ATCTCAGAAC GTGAAAGAAT GATGAATCTG 
GAGAACCAGA GGAGGGCACA GGAGCGTGAC CGATTACATG GAGGTGGAGG GGTTGCACCT 
GACCCATCTG CGTATATGGA TGTGTCTGGA TCTGCAAGGG TACCAGGAAG GGAGCCAGGG 
ATGATGGGAG TTCCCAGCTC ACCCAATTCA GGCCGAAGGG GCTTGATGCT CTCTCCCAAA 
CAGGGAGCGT CTCCTGGTGA TCTGTTTATT GAGCAGCAGG CTCACAGCAT CCTCCAAAGA 
CACGGTCAGT CATCCAACCA ATGGTCCCTT CAGACCGATT CAGAAGGAGA CACCGTCTCT 
CCAATCAACT GGCCTTCCAG AATCAGAGTT AGAGAGCGAC TGGCCCGCGA TGAGCCATTT 
CACAGGGGTC AAACAACAGA GGAACCTGCT TACAACCAGT CATATCTTCG TTATTACCCT 
CACTCACAGA GCCTCCTAGA TGGTCCTGTC AAACAGCAGC AACTGACCCG TTCTGAAAAA 
AAGGAACGCC ATGATATGGG TTGCCAGAGT TACTTCTCCA TGGGCAGCGA ATCCACTTCC 
AGGAATAAGC AAGAGATTCA GGAGGTGGTT GAAGAAATGG AGGGAAAACA TGTTGCGAGG 
ACTGAAGGTC TTCTGCGGAG CAGAAAGGCA GTTTTACCTT CTGAGATTCG AAGGAAGGAA 
CGGAACACAG ACGATTCATG CTCTGGAAGT GGTGACAACA GGGAGGTAAG CTCAGCTATT 
GCTGGGCACA GGAGCAGCGA GGGTGCTGCA AATGACCCCA GAGGGTGGCA TGGAAGTCGG 
GCAAGACGGG AAGTTTATAC AGAGCACGTG TCTCGACTTC AGGCTGCAGA GTCTTTGCAG 
GCAAGAGCTC GAGAAGCTCA TAGAAGGCCT GATGCTAGCT GCTGCGCCCC AATCCAGACC 
TCCAGTGCAA GACCAATGAC AAATTCATCT AAACCCATGG ATACTGGAAA TACACAAAAA 
ACTGTTCTCC CCATCCAGGA GCCAATCAGA GAGGCTGTCC AACAAGAAAA AGTGCCAAAT 
GTTGATCACC TGTCCCATGA TGCCAGGGTT TCAGTAGCCC ATCTCCGCCA CACATACCTG 
CAAAGTGTTG CAAGTCCCCG GAAAGCCGAA CTGGCAGAAG AGCTGCCTGC AGTTACAGAT 
GTACCCTGGA GAAGCATGCG AGTCAGAGAT CGGTCATCTC GTCGCCCTTT GCGATATATA 
TGTCCCACTG AAAGTAGAAA GACATCTGAG AGGTTTAGAA CACAGCCAAT CACATCAACC 
GAGCGTGAGC AGTCGGACCG GTCTCATCTA AGCTCTGAAC CATCAAGCCC TGAGGTGGAT 
GAAGAGAAAC TAGATGAGAG AGCAAAGCTG AGCGTAGCTG CCAAGCGTTC CCTCTTCAGG 
GAGCTTGAAA AGACTTCAGA TGGTTCCGTT TTAAAGACAT GGAGTCGTAA TCCTGCTGTT 
GAGCGGAGAT TGAGGAGAGG TCAAGATCGA TCCCGCACTC AGCCAGTCAC CACAGAGGAA 
GTAGTTATTG CTGCTACTGC CCCTGGCCAT TTGTCACAGT CCGTGACTTT ACAAACCTCT 
ATAACGCGCG TTCCTAGTCC CACTCTAGTC AGCACCTCTG TGACCAAACA CAGCTTGCAG 
GCTTCGTCTC AGCAAAGCTC CATGGTGAGA GAGCAGGCCA GAGAGGTTCG TCTGGCTCAG 
GAGGCAACGG AGACAGATCG AGCTCGCCAC GCTTCTCCGG GGGAAGGCGG ACACGAGGAG 
CCAGACCTTT CCACACTCAC TCTAGCAGAA AAGATGGCAC TTTTTAACCG TCTTGCCCAG 
CCGTCCAGCC ACGGCTCTAG GGCCAGAGGA GACACGCGTG CACGGAGGAG TAACGCACGC 
TATCAGACTC AACCAATCAC ACTAGGAGAA GTCGAACAGT TTCAGCCTGC AGGTGGAGCT 
CGTTTTGCAC CCAGCTCCAA CTCAGCCATG AGATCCTCCA CTGTTTCCAC TGAACATGCG 
GGCGACATCC ATCTGACCAT GCCAAATGTA TCTGGGCCTG CATATTATGA GCAGACCGTT 
TCTCCACCGC ATCACCCTCA GCCTGCCCAT GAGATCCAGA CACATCAGAG TCTGGCTGAA 
GGGGAAAGGC TGCTGTTGAG CACTGGGAAG AGGAAGCTTC CCTCCCCAGA CAGCGTGAAA 
CGGACACCGC ATGCTCAGCC GGCCAGAGAC TGGGACAGTA AGGCTGTGGC GGTGACCTCT 
CAAGACCTTT CACAAACAGA TGGAGAGCAC ACAACAGATC TTCCTGTACA CACTGCCGCT 
GCTGTATCTG CATTACAGAG CCATTCTCTC CATGAGGACC ACACCAGAGA GGAAGAGCAG 
GAGAGCGACT TCAAAACAGA AGACGGAGAC GAGCTCTCGG ACGTCATGTC TGCCAGACAG 
ATGTCCATCA AAGAGAGGCT GGCTCTGCTG AAGAAGAGTG GAGAGGAAGA CTGGAGGAAC 
AGGATAAATA AAAAACAGGA TGTGGTGAAG GTTGCTGTGT CTGAAAGACA TGCCCAGCTA 
TGGGAGGTGG AGCAGAGCTT CAAGAAGAAG GATGATGGGA TGATGATAGA TGACTTTGCT 
GTGTCTGATC AACTCTGGGA TCCTGTCTTT TCCTCCTCCC TTTTGTGTGC CTCTGAACCA 
CTGGATCAAA TAGCAAACCT TCCCAAGGCT ACCCTCCTGC CCGTGCAGGT GGATGAGCGT 
CACCCCTGGA GACGGAAGAA GATGGGAAAT GAAGTTGTGG AAAGCCAACA GCTTGAGCGA 
GTAGAGATGT CTATTCAGGA ACGCAAGCAG CTCATCACTT CACAAGAGGA GGCCTGGAAA 
GCCAAAGGTT ACGGAGCAGC CAACGACTCC ACTCAGTTTA CAGTGGCTGG ACGTATGGTC 
AAAAAAGGCC TGGCAGCCCC TTCTGTTCTC ACAAATCCCG TGTTGTCTCC TCTGTCTTCT 
AAAAACAAGA ATACATCCCA TACCATCACA AAACCACATG AAGAGATTGA AGTCAAACAG 
AACATGAAGC TGGAGTCAGA CATGAAGTTA GAAAAGTTGG AAACATTCCT TGGTCGTTTG 
AATAGTAAAG TTCCAGGACT TCAGGAGGCC ACAATTGCTG TCACGGAGAA GTCTGTAAAA 
GAGGTCATGA CTCTGGACGA TGAGGTCTTT GATAAGTTTT ATCGACATGC AGAGGATATG 
GCAAAGTTCA CCAGCAGAGT GGAAATTAGT GATGATTTTG ATGACATCTT TGGAGTACAG 
CTGCCCAGGT TGACTTCGGA AATGGTGCAG CACAAGCGGG CAGTTCGGCC TACCAGAAAT 
GTTCAGTCAT CTAAAAACCC GCTGAAAATG CTGGCTGTTC GGGAGGACAT CAGACATGAG 
TACACGGAAC AAAGACTGAA CATCGGCCTT CTGGAGAGCA AGAGGATGAA ACAGGAAAAG 
ATGAATAAGA ACAGCGGTTT CTCTGAGGTT GCTCTCGCCG GATTGGCCAG TAAGGAGAAC 
TTCAGCAGTG TGAGTCTGCG AAGTGTGAAT GCCTCTGAAC AAAGCTCCAA CAACAGTGCC 
ATGCCCTATA AAAAACTCAT GCTCATTCAG GTCAAAGGTC GCAGGCACGT TCAGACCAGA 
CTGGTTGAGC CCAGAGCTTC CTCCTTGAAC AGTGGCGACT GCTTCCTCCT GATCACACCA 
CATCATTGCT TCATCTGGAT TGGAGAATTC GCTAATGTTA TTGAGAAGGC CAAAGCTGCA 
GAGTTGGCCA CGTTCGTTCA AACCAAGCAT GACCTGGGAT GCAGAGCCTC GTATGTTCAG 
ACAATTGAGG AGGGTGCAAA CACTCACACA CACGCTGCCA AAGACTTTTG GAAGATTCTC 
GGTGGCCAAA CCAGTTATCA GTCCGCTGGC ACTCCTGAAC AAGATGAGTT TTATGAGAGT 
GCAGTCGTAG AAACCAACTG CATATACAGA CTCATGGAGG ATAAACTTGT TCCCCATGAT 
GACTACTGGG GTCGAGTCCC TCGCTGCTCC ATGCTGAACC CTAAAGAGGT GCTGGTATTT 
GATTTTGGTA GTGAGGTCTA TGTATGGCAT GGCAAGGAGG TGACGTTGGC ACAGAGAAAA 
GTGGCATTTC AGCTGGCGAA GCACTTGTGG AATGGCACCT TTGACTACAC CAACTGTGAC 
ATTAATCCTC TGGACCCTGG AGAGTGCAAT GCTCTCATAC CCAGGAAAGG CCAGGGACGA 
CCAGACTGGG CTGTGTTTGG CAGGTTAACT CAGCACAATG AAACTACTCT TTTTAAAGAG 
AAGTTTTTGG ACTGGAGTGA TTCCAAGAAT TCTAAGAAAA ATGGTGACTT GCATGTTGAA 
AACAAGGAGC TTCGGAGTGG TGAATGTCGG CCCTATGATG CGTCTCTGAT GCTATCTGCA 
TTACGGGCGC CAGTCAGGAC CGTTCTGGAT GGGGTGAACA TTGGACGAGG ATATGGATTA 
GTGGAAGGTG AAGAGGGACG TAATTTTGAG ATCAGCACAT TGTCTGTGGA TGTCTGGCAT 
ATTTTAGAGT TTGACTACAG TCGACTACCC AAACAGAGCA TTGGTCAGTT TCATGAAGGT 
GACACATACG TGGTCAAATG GAAGTACATG ATCAGCAGAG CAGTTGGCAA GAGACTGCAT 
TCAGAAAGAA TCATCGGTCC AGGGAAGGAG AAATGTTGTT ATTTCTTCTG GCAGGGTCGT 
AACTCTACGG TGAATGAAAA GGGAACGTCA GCACTGATGA CTGTTGAGCT GGATGAGGAA 
AGAGGAGCTC AGGTCCAGGT ACAGCAAGGA AAGGAGCCTC CTTGTTTCCT ACAGTGCTTC 
AATGGAGGAA TGATTGTTCA TGCTGGAAAA AGAGAAGAGG AGGAAGACAA TACACAAAAC 
GACTGGCGAT TGTACTGTGT GAGAGGAGAG AAACCCATAG AGGGCCATCT GCTGGAGGCA 
GTGTGTCACT GCAGCAGCTT GCGATCCCGG ACCTCTATGA TCCTCCTGAA CATCCCCAAA 
GCCAGCCTAT ACCTGTGGCA TGGCTGCAAG GCACAAGTGC ACACACGTGA CGTGGGCCGT 
ACCACTGCAA ACAAAATCAA AGAGCAATGT CCTCTGGAGG CAGGGCTTCA CAGCAGCAGT 
AAAGTGTCCA TCCAGGAGTG TGATGAGGGA GCTGAACCAC AGGGCTTCTG GGAGGCTTTG 
GGCAAAAGAG ATCGAAAAGC GTACGACTGC ATGCTGCAGG ATCCAGGAAA GTTCAACTTC 
ACTCCTCGGC TTTTCCAGTT AAGCAGTGCC TCGGGAGAGT TTGCAGCAGT GGAGTTTGTA 
TATCCATCCA GAGAACCAAA TTTGGTTAAC TCGATGCCTT TCCTCCAGGA GGATTTGTAT 
ACAGCTACTC AGCCAGCCCT GTTTTTGGTG GATAATCATC ATGAAGTTTA CCTGTGGCAG 
GGCTGGTGGC CTCAGGACAG TGAAAGCACA GGCTCAGCTC GCATACGGTG GGACTCTGAC 
AGGAAGTGTG CAATGGAAAC TGTGCTCCAG TATAGCAAAG AAAAAAATGA GAAAAAGACA 
CCAAAATCCT ACCTGATCCA TGCAGGACTG GAGCCTCTTA CATTCACCAA CATGTTTCCC 
AGCTGGGAAC ACAGAGAGGA CATCGCTGAA ATCACAGAGA AGGAGGCCGA AGTATGTCAT 
CAGATCATTC TTGTTGAAGA TGTCTTGAGC CGACTCTGCA AGACCACTTA CCCACTGGCT 
GAACTAAAAG CACGTCCCTT ACCGGAAGGT GTCGACCCTC TACGTCTTGA AACCTACTTA 
TCAGATGAGG ACTTTGAGGG TGTAGGGGCT TCTGGGAAGA AAGCTTTGGA GATGACGAGA 
AGTGAATATG AGGCACTGCC TGGATGGAAG CAAGTGAATG TGAAGAAAGC CAAAGGATTG 
TTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

svila ( XM_021480013.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021335688.1 Danio rerio supervillin a (svila), transcript variant X3, mRNA.

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CloneID	ODa148131
Clone ID Related Accession (Same CDS sequence)	XM_021480013.1
Accession Version	XM_021480013.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6720bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	supervillin isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007123.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTATCGGA ACCCTTGGAT CTCCAACTAT ATCAACCATG TCAAGTTGTG CCAAGGCTTC 
ACCAGAGAAA CAGCCCCTAA AGTTGGCAAT ACCAAGCAAC GGAGAGCTCT TAGTTTTCAG 
AAAGAGTTCT CGTTTGAGGA GAAAGGTGAG GGAGCCAACA AAGAAGTAGA TGTTAGTCTG 
CTGGGGAATA CACCCAAGGT CTCAGAATTA AGGAAACGTT TTGAAAGTAT AAGCACAAGT 
CAGAGTTGGG ACATGAACAG GAAGGAGCGA ATTGCACGGC GCCTGGAAGG AATCGATAAT 
GATGTTCTCC AAAGTCTTCC AGGTTGTGGT GGTCTAGTTT CTAATCGACT GCTGGAGGAG 
GACACTCCAA GATATACCCG TGCAGCAGAC ACCTGTGACC CCTGTACAGT TTCTCTTCAG 
CACTATGGCA AAAGTGAACC AAGTTCACAA AGCACAGATG TTCCATCCAG AAGTTACGCA 
GTCCACCCGG AGTCTGTCTT CAGCACTGGC TCCTCTCATG TGGCAGAGCT GGACTCTAAA 
GCAGAAAGGA TTGCACGCTA CAAGGCTGAA CGCCGCCGTC AGTTAGCAGA GCGCTATGGC 
ATCTCTCTAG ATCAGGAGAT GGAAACAGAC TATACAACTC GCTTCTCCAG ACCCTCCAGA 
GAGCTTGATG GTTCAGACAG ACACCACAGG TCAAAATCAG AAGGGAGAGA TGGAGTCGAG 
CAAAATGCTT ACACCAGCAG CCATCATGTG GATAAGAGCA GCTCAGACTA TCACTCCAGT 
CCAGAGTACA GCCGCGAGCG CACAGACTCC ATCTCAGAAC GTGAAAGAAT GATGAATCTG 
GAGAACCAGA GGAGGGCACA GGAGCGTGAC CGATTACATG GAGGTGGAGG GGTTGCACCT 
GACCCATCTG CGTATATGGA TGTGTCTGGA TCTGCAAGGG TACCAGGAAG GGAGCCAGGG 
ATGATGGGAG TTCCCAGCTC ACCCAATTCA GGCCGAAGGG GCTTGATGCT CTCTCCCAAA 
CAGGGAGCGT CTCCTGGTGA TCTGTTTATT GAGCAGCAGG CTCACAGCAT CCTCCAAAGA 
CACGGAATCA GAGTTAGAGA GCGACTGGCC CGCGATGAGC CATTTCACAG GGGTCAAACA 
ACAGAGGAAC CTGCTTACAA CCAGTCATAT CTTCGTTATT ACCCTCACTC ACAGAGCCTC 
CTAGATGGTC CTGTCAAACA GCAGCAACTG ACCCGTTCTG AAAAAAAGGA ACGCCATGAT 
ATGGGTTGCC AGAGTTACTT CTCCATGGGC AGCGAATCCA CTTCCAGGAA TAAGCAAGAG 
ATTCAGGAGG TGGTTGAAGA AATGGAGGGA AAACATGTTG CGAGGACTGA AGGTCTTCTG 
CGGAGCAGAA AGGCAGTTTT ACCTTCTGAG ATTCGAAGGA AGGAACGGAA CACAGACGAT 
TCATGCTCTG GAAGTGGTGA CAACAGGGAG GTAAGCTCAG CTATTGCTGG GCACAGGAGC 
AGCGAGGGTG CTGCAAATGA CCCCAGAGGG TGGCATGGAA GTCGGGCAAG ACGGGAAGTT 
TATACAGAGC ACGTGTCTCG ACTTCAGGCT GCAGAGTCTT TGCAGGCAAG AGCTCGAGAA 
GCTCATAGAA GGCCTGATGC TAGCTGCTGC GCCCCAATCC AGACCTCCAG TGCAAGACCA 
ATGACAAATT CATCTAAACC CATGGATACT GGAAATACAC AAAAAACTGT TCTCCCCATC 
CAGGAGCCAA TCAGAGAGGC TGTCCAACAA GAAAAAGTGC CAAATGTTGA TCACCTGTCC 
CATGATGCCA GGGTTTCAGT AGCCCATCTC CGCCACACAT ACCTGCAAAG TGTTGCAAGT 
CCCCGGAAAG CCGAACTGGC AGAAGAGCTG CCTGCAGTTA CAGATGTACC CTGGAGAAGC 
ATGCGAGTCA GAGATCGGTC ATCTCGTCGC CCTTTGCGAT ATATATGTCC CACTGAAAGT 
AGAAAGACAT CTGAGAGGTT TAGAACACAG CCAATCACAT CAACCGAGCG TGAGCAGTCG 
GACCGGTCTC ATCTAAGCTC TGAACCATCA AGCCCTGAGG TGGATGAAGA GAAACTAGAT 
GAGAGAGCAA AGCTGAGCGT AGCTGCCAAG CGTTCCCTCT TCAGGGAGCT TGAAAAGACT 
TCAGATGGTT CCGTTTTAAA GACATGGAGT CGTAATCCTG CTGTTGAGCG GAGATTGAGG 
AGAGGTCAAG ATCGATCCCG CACTCAGCCA GTCACCACAG AGGAAGTAGT TATTGCTGCT 
ACTGCCCCTG GCCATTTGTC ACAGTCCGTG ACTTTACAAA CCTCTATAAC GCGCGTTCCT 
AGTCCCACTC TAGTCAGCAC CTCTGTGACC AAACACAGCT TGCAGGCTTC GTCTCAGCAA 
AGCTCCATGG TGAGAGAGCA GGCCAGAGAG GTTCGTCTGG CTCAGGAGGC AACGGAGACA 
GATCGAGCTC GCCACGCTTC TCCGGGGGAA GGCGGACACG AGGAGCCAGA CCTTTCCACA 
CTCACTCTAG CAGAAAAGAT GGCACTTTTT AACCGTCTTG CCCAGCCGTC CAGCCACGGC 
TCTAGGGCCA GAGGAGACAC GCGTGCACGG AGGAGTAACG CACGCTATCA GACTCAACCA 
ATCACACTAG GAGAAGTCGA ACAGGATGGT TCTGGTTCTG AGATGTTCGA TGAGGAATCT 
GAGTTCTCAA TGTGTCTCAG TGATCTGGAA CATTTTCAGC CTGCAGGTGG AGCTCGTTTT 
GCACCCAGCT CCAACTCAGC CATGAGATCC TCCACTGTTT CCACTGAACA TGCGGGCGAC 
ATCCATCTGA CCATGCCAAA TGTATCTGGG CCTGCATATT ATGAGCAGAC CGTTTCTCCA 
CCGCATCACC CTCAGCCTGC CCATGAGATC CAGACACATC AGAGTCTGGC TGAAGGGGAA 
AGGCTGCTGT TGAGCACTGG GAAGAGGAAG CTTCCCTCCC CAGACAGCGT GAAACGGACA 
CCGCATGCTC AGCCGGCCAG AGACTGGGAC AGTAAGGCTG TGGCGGTGAC CTCTCAAGAC 
CTTTCACAAA CAGATGGAGA GCACACAACA GATCTTCCTG TACACACTGC CGCTGCTGTA 
TCTGCATTAC AGAGCCATTC TCTCCATGAG GACCACACCA GAGAGGAAGA GCAGGAGAGC 
GACTTCAAAA CAGAAGACGG AGACGAGCTC TCGGACGTCA TGTCTGCCAG ACAGATGTCC 
ATCAAAGAGA GGCTGGCTCT GCTGAAGAAG AGTGGAGAGG AAGACTGGAG GAACAGGATA 
AATAAAAAAC AGGATGTGGT GAAGGTTGCT GTGTCTGAAA GACATGCCCA GCTATGGGAG 
GTGGAGCAGA GCTTCAAGAA GAAGGATGAT GGGATGATGA TAGATGACTT TGCTGTGTCT 
GATCAACTCT GGGATCCTGT CTTTTCCTCC TCCCTTTTGT GTGCCTCTGA ACCACTGGAT 
CAAATAGCAA ACCTTCCCAA GGCTACCCTC CTGCCCGTGC AGGTGGATGA GCGTCACCCC 
TGGAGACGGA AGAAGATGGG AAATGAAGTT GTGGAAAGCC AACAGCTTGA GCGAGTAGAG 
ATGTCTATTC AGGAACGCAA GCAGCTCATC ACTTCACAAG AGGAGGCCTG GAAAGCCAAA 
GGTTACGGAG CAGCCAACGA CTCCACTCAG TTTACAGTGG CTGGACGTAT GGTCAAAAAA 
GGCCTGGCAG CCCCTTCTGT TCTCACAAAT CCCGTGTTGT CTCCTCTGTC TTCTAAAAAC 
AAGAATACAT CCCATACCAT CACAAAACCA CATGAAGAGA TTGAAGTCAA ACAGAACATG 
AAGCTGGAGT CAGACATGAA GTTAGAAAAG TTGGAAACAT TCCTTGGTCG TTTGAATAGT 
AAAGTTCCAG GACTTCAGGA GGCCACAATT GCTGTCACGG AGAAGTCTGT AAAAGAGGTC 
ATGACTCTGG ACGATGAGGT CTTTGATAAG TTTTATCGAC ATGCAGAGGA TATGGCAAAG 
TTCACCAGCA GAGTGGAAAT TAGTGATGAT TTTGATGACA TCTTTGGAGT ACAGCTGCCC 
AGGTTGACTT CGGAAATGGT GCAGCACAAG CGGGCAGTTC GGCCTACCAG AAATGTTCAG 
TCATCTAAAA ACCCGCTGAA AATGCTGGCT GTTCGGGAGG ACATCAGACA TGAGTACACG 
GAACAAAGAC TGAACATCGG CCTTCTGGAG AGCAAGAGGA TGAAACAGGA AAAGATGAAT 
AAGAACAGCG GTTTCTCTGA GGTTGCTCTC GCCGGATTGG CCAGTAAGGA GAACTTCAGC 
AGTGTGAGTC TGCGAAGTGT GAATGCCTCT GAACAAAGCT CCAACAACAG TGCCATGCCC 
TATAAAAAAC TCATGCTCAT TCAGGTCAAA GGTCGCAGGC ACGTTCAGAC CAGACTGGTT 
GAGCCCAGAG CTTCCTCCTT GAACAGTGGC GACTGCTTCC TCCTGATCAC ACCACATCAT 
TGCTTCATCT GGATTGGAGA ATTCGCTAAT GTTATTGAGA AGGCCAAAGC TGCAGAGTTG 
GCCACGTTCG TTCAAACCAA GCATGACCTG GGATGCAGAG CCTCGTATGT TCAGACAATT 
GAGGAGGGTG CAAACACTCA CACACACGCT GCCAAAGACT TTTGGAAGAT TCTCGGTGGC 
CAAACCAGTT ATCAGTCCGC TGGCACTCCT GAACAAGATG AGTTTTATGA GAGTGCAGTC 
GTAGAAACCA ACTGCATATA CAGACTCATG GAGGATAAAC TTGTTCCCCA TGATGACTAC 
TGGGGTCGAG TCCCTCGCTG CTCCATGCTG AACCCTAAAG AGGTGCTGGT ATTTGATTTT 
GGTAGTGAGG TCTATGTATG GCATGGCAAG GAGGTGACGT TGGCACAGAG AAAAGTGGCA 
TTTCAGCTGG CGAAGCACTT GTGGAATGGC ACCTTTGACT ACACCAACTG TGACATTAAT 
CCTCTGGACC CTGGAGAGTG CAATGCTCTC ATACCCAGGA AAGGCCAGGG ACGACCAGAC 
TGGGCTGTGT TTGGCAGGTT AACTCAGCAC AATGAAACTA CTCTTTTTAA AGAGAAGTTT 
TTGGACTGGA GTGATTCCAA GAATTCTAAG AAAAATGGTG ACTTGCATGT TGAAAACAAG 
GAGCTTCGGA GTGGTGAATG TCGGCCCTAT GATGCGTCTC TGATGCTATC TGCATTACGG 
GCGCCAGTCA GGACCGTTCT GGATGGGGTG AACATTGGAC GAGGATATGG ATTAGTGGAA 
GGTGAAGAGG GACGTAATTT TGAGATCAGC ACATTGTCTG TGGATGTCTG GCATATTTTA 
GAGTTTGACT ACAGTCGACT ACCCAAACAG AGCATTGGTC AGTTTCATGA AGGTGACACA 
TACGTGGTCA AATGGAAGTA CATGATCAGC AGAGCAGTTG GCAAGAGACT GCATTCAGAA 
AGAATCATCG GTCCAGGGAA GGAGAAATGT TGTTATTTCT TCTGGCAGGG TCGTAACTCT 
ACGGTGAATG AAAAGGGAAC GTCAGCACTG ATGACTGTTG AGCTGGATGA GGAAAGAGGA 
GCTCAGGTCC AGGTACAGCA AGGAAAGGAG CCTCCTTGTT TCCTACAGTG CTTCAATGGA 
GGAATGATTG TTCATGCTGG AAAAAGAGAA GAGGAGGAAG ACAATACACA AAACGACTGG 
CGATTGTACT GTGTGAGAGG AGAGAAACCC ATAGAGGGCC ATCTGCTGGA GGCAGTGTGT 
CACTGCAGCA GCTTGCGATC CCGGACCTCT ATGATCCTCC TGAACATCCC CAAAGCCAGC 
CTATACCTGT GGCATGGCTG CAAGGCACAA GTGCACACAC GTGACGTGGG CCGTACCACT 
GCAAACAAAA TCAAAGAGCA ATGTCCTCTG GAGGCAGGGC TTCACAGCAG CAGTAAAGTG 
TCCATCCAGG AGTGTGATGA GGGAGCTGAA CCACAGGGCT TCTGGGAGGC TTTGGGCAAA 
AGAGATCGAA AAGCGTACGA CTGCATGCTG CAGGATCCAG GAAAGTTCAA CTTCACTCCT 
CGGCTTTTCC AGTTAAGCAG TGCCTCGGGA GAGTTTGCAG CAGTGGAGTT TGTATATCCA 
TCCAGAGAAC CAAATTTGGT TAACTCGATG CCTTTCCTCC AGGAGGATTT GTATACAGCT 
ACTCAGCCAG CCCTGTTTTT GGTGGATAAT CATCATGAAG TTTACCTGTG GCAGGGCTGG 
TGGCCTCAGG ACAGTGAAAG CACAGGCTCA GCTCGCATAC GGTGGGACTC TGACAGGAAG 
TGTGCAATGG AAACTGTGCT CCAGTATAGC AAAGAAAAAA ATGAGAAAAA GACACCAAAA 
TCCTACCTGA TCCATGCAGG ACTGGAGCCT CTTACATTCA CCAACATGTT TCCCAGCTGG 
GAACACAGAG AGGACATCGC TGAAATCACA GAGAAGGAGG CCGAAGTATG TCATCAGATC 
ATTCTTGTTG AAGATGTCTT GAGCCGACTC TGCAAGACCA CTTACCCACT GGCTGAACTA 
AAAGCACGTC CCTTACCGGA AGGTGTCGAC CCTCTACGTC TTGAAACCTA CTTATCAGAT 
GAGGACTTTG AGGGTGTAGG GGCTTCTGGG AAGAAAGCTT TGGAGATGAC GAGAAGTGAA 
TATGAGGCAC TGCCTGGATG GAAGCAAGTG AATGTGAAGA AAGCCAAAGG ATTGTTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021335688.1
CDS	343..7062
Translation	MYRNPWISNYINHVKLCQGFTRETAPKVGNTKQRRALSFQKEFSFEEKGEGANKEVDVSLLGNTPKVSELRKRFESISTSQSWDMNRKERIARRLEGIDNDVLQSLPGCGGLVSNRLLEEDTPRYTRAADTCDPCTVSLQHYGKSEPSSQSTDVPSRSYAVHPESVFSTGSSHVAELDSKAERIARYKAERRRQLAERYGISLDQEMETDYTTRFSRPSRELDGSDRHHRSKSEGRDGVEQNAYTSSHHVDKSSSDYHSSPEYSRERTDSISERERMMNLENQRRAQERDRLHGGGGVAPDPSAYMDVSGSARVPGREPGMMGVPSSPNSGRRGLMLSPKQGASPGDLFIEQQAHSILQRHGIRVRERLARDEPFHRGQTTEEPAYNQSYLRYYPHSQSLLDGPVKQQQLTRSEKKERHDMGCQSYFSMGSESTSRNKQEIQEVVEEMEGKHVARTEGLLRSRKAVLPSEIRRKERNTDDSCSGSGDNREVSSAIAGHRSSEGAANDPRGWHGSRARREVYTEHVSRLQAAESLQARAREAHRRPDASCCAPIQTSSARPMTNSSKPMDTGNTQKTVLPIQEPIREAVQQEKVPNVDHLSHDARVSVAHLRHTYLQSVASPRKAELAEELPAVTDVPWRSMRVRDRSSRRPLRYICPTESRKTSERFRTQPITSTEREQSDRSHLSSEPSSPEVDEEKLDERAKLSVAAKRSLFRELEKTSDGSVLKTWSRNPAVERRLRRGQDRSRTQPVTTEEVVIAATAPGHLSQSVTLQTSITRVPSPTLVSTSVTKHSLQASSQQSSMVREQAREVRLAQEATETDRARHASPGEGGHEEPDLSTLTLAEKMALFNRLAQPSSHGSRARGDTRARRSNARYQTQPITLGEVEQDGSGSEMFDEESEFSMCLSDLEHFQPAGGARFAPSSNSAMRSSTVSTEHAGDIHLTMPNVSGPAYYEQTVSPPHHPQPAHEIQTHQSLAEGERLLLSTGKRKLPSPDSVKRTPHAQPARDWDSKAVAVTSQDLSQTDGEHTTDLPVHTAAAVSALQSHSLHEDHTREEEQESDFKTEDGDELSDVMSARQMSIKERLALLKKSGEEDWRNRINKKQDVVKVAVSERHAQLWEVEQSFKKKDDGMMIDDFAVSDQLWDPVFSSSLLCASEPLDQIANLPKATLLPVQVDERHPWRRKKMGNEVVESQQLERVEMSIQERKQLITSQEEAWKAKGYGAANDSTQFTVAGRMVKKGLAAPSVLTNPVLSPLSSKNKNTSHTITKPHEEIEVKQNMKLESDMKLEKLETFLGRLNSKVPGLQEATIAVTEKSVKEVMTLDDEVFDKFYRHAEDMAKFTSRVEISDDFDDIFGVQLPRLTSEMVQHKRAVRPTRNVQSSKNPLKMLAVREDIRHEYTEQRLNIGLLESKRMKQEKMNKNSGFSEVALAGLASKENFSSVSLRSVNASEQSSNNSAMPYKKLMLIQVKGRRHVQTRLVEPRASSLNSGDCFLLITPHHCFIWIGEFANVIEKAKAAELATFVQTKHDLGCRASYVQTIEEGANTHTHAAKDFWKILGGQTSYQSAGTPEQDEFYESAVVETNCIYRLMEDKLVPHDDYWGRVPRCSMLNPKEVLVFDFGSEVYVWHGKEVTLAQRKVAFQLAKHLWNGTFDYTNCDINPLDPGECNALIPRKGQGRPDWAVFGRLTQHNETTLFKEKFLDWSDSKNSKKNGDLHVENKELRSGECRPYDASLMLSALRAPVRTVLDGVNIGRGYGLVEGEEGRNFEISTLSVDVWHILEFDYSRLPKQSIGQFHEGDTYVVKWKYMISRAVGKRLHSERIIGPGKEKCCYFFWQGRNSTVNEKGTSALMTVELDEERGAQVQVQQGKEPPCFLQCFNGGMIVHAGKREEEEDNTQNDWRLYCVRGEKPIEGHLLEAVCHCSSLRSRTSMILLNIPKASLYLWHGCKAQVHTRDVGRTTANKIKEQCPLEAGLHSSSKVSIQECDEGAEPQGFWEALGKRDRKAYDCMLQDPGKFNFTPRLFQLSSASGEFAAVEFVYPSREPNLVNSMPFLQEDLYTATQPALFLVDNHHEVYLWQGWWPQDSESTGSARIRWDSDRKCAMETVLQYSKEKNEKKTPKSYLIHAGLEPLTFTNMFPSWEHREDIAEITEKEAEVCHQIILVEDVLSRLCKTTYPLAELKARPLPEGVDPLRLETYLSDEDFEGVGASGKKALEMTRSEYEALPGWKQVNVKKAKGLF

Target ORF information:

RefSeq Version	XM_021480013.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio supervillin a (svila), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021480013.1

ORF Insert Sequence:

ATGTATCGGA ACCCTTGGAT CTCCAACTAT ATCAACCATG TCAAGTTGTG CCAAGGCTTC 
ACCAGAGAAA CAGCCCCTAA AGTTGGCAAT ACCAAGCAAC GGAGAGCTCT TAGTTTTCAG 
AAAGAGTTCT CGTTTGAGGA GAAAGGTGAG GGAGCCAACA AAGAAGTAGA TGTTAGTCTG 
CTGGGGAATA CACCCAAGGT CTCAGAATTA AGGAAACGTT TTGAAAGTAT AAGCACAAGT 
CAGAGTTGGG ACATGAACAG GAAGGAGCGA ATTGCACGGC GCCTGGAAGG AATCGATAAT 
GATGTTCTCC AAAGTCTTCC AGGTTGTGGT GGTCTAGTTT CTAATCGACT GCTGGAGGAG 
GACACTCCAA GATATACCCG TGCAGCAGAC ACCTGTGACC CCTGTACAGT TTCTCTTCAG 
CACTATGGCA AAAGTGAACC AAGTTCACAA AGCACAGATG TTCCATCCAG AAGTTACGCA 
GTCCACCCGG AGTCTGTCTT CAGCACTGGC TCCTCTCATG TGGCAGAGCT GGACTCTAAA 
GCAGAAAGGA TTGCACGCTA CAAGGCTGAA CGCCGCCGTC AGTTAGCAGA GCGCTATGGC 
ATCTCTCTAG ATCAGGAGAT GGAAACAGAC TATACAACTC GCTTCTCCAG ACCCTCCAGA 
GAGCTTGATG GTTCAGACAG ACACCACAGG TCAAAATCAG AAGGGAGAGA TGGAGTCGAG 
CAAAATGCTT ACACCAGCAG CCATCATGTG GATAAGAGCA GCTCAGACTA TCACTCCAGT 
CCAGAGTACA GCCGCGAGCG CACAGACTCC ATCTCAGAAC GTGAAAGAAT GATGAATCTG 
GAGAACCAGA GGAGGGCACA GGAGCGTGAC CGATTACATG GAGGTGGAGG GGTTGCACCT 
GACCCATCTG CGTATATGGA TGTGTCTGGA TCTGCAAGGG TACCAGGAAG GGAGCCAGGG 
ATGATGGGAG TTCCCAGCTC ACCCAATTCA GGCCGAAGGG GCTTGATGCT CTCTCCCAAA 
CAGGGAGCGT CTCCTGGTGA TCTGTTTATT GAGCAGCAGG CTCACAGCAT CCTCCAAAGA 
CACGGAATCA GAGTTAGAGA GCGACTGGCC CGCGATGAGC CATTTCACAG GGGTCAAACA 
ACAGAGGAAC CTGCTTACAA CCAGTCATAT CTTCGTTATT ACCCTCACTC ACAGAGCCTC 
CTAGATGGTC CTGTCAAACA GCAGCAACTG ACCCGTTCTG AAAAAAAGGA ACGCCATGAT 
ATGGGTTGCC AGAGTTACTT CTCCATGGGC AGCGAATCCA CTTCCAGGAA TAAGCAAGAG 
ATTCAGGAGG TGGTTGAAGA AATGGAGGGA AAACATGTTG CGAGGACTGA AGGTCTTCTG 
CGGAGCAGAA AGGCAGTTTT ACCTTCTGAG ATTCGAAGGA AGGAACGGAA CACAGACGAT 
TCATGCTCTG GAAGTGGTGA CAACAGGGAG GTAAGCTCAG CTATTGCTGG GCACAGGAGC 
AGCGAGGGTG CTGCAAATGA CCCCAGAGGG TGGCATGGAA GTCGGGCAAG ACGGGAAGTT 
TATACAGAGC ACGTGTCTCG ACTTCAGGCT GCAGAGTCTT TGCAGGCAAG AGCTCGAGAA 
GCTCATAGAA GGCCTGATGC TAGCTGCTGC GCCCCAATCC AGACCTCCAG TGCAAGACCA 
ATGACAAATT CATCTAAACC CATGGATACT GGAAATACAC AAAAAACTGT TCTCCCCATC 
CAGGAGCCAA TCAGAGAGGC TGTCCAACAA GAAAAAGTGC CAAATGTTGA TCACCTGTCC 
CATGATGCCA GGGTTTCAGT AGCCCATCTC CGCCACACAT ACCTGCAAAG TGTTGCAAGT 
CCCCGGAAAG CCGAACTGGC AGAAGAGCTG CCTGCAGTTA CAGATGTACC CTGGAGAAGC 
ATGCGAGTCA GAGATCGGTC ATCTCGTCGC CCTTTGCGAT ATATATGTCC CACTGAAAGT 
AGAAAGACAT CTGAGAGGTT TAGAACACAG CCAATCACAT CAACCGAGCG TGAGCAGTCG 
GACCGGTCTC ATCTAAGCTC TGAACCATCA AGCCCTGAGG TGGATGAAGA GAAACTAGAT 
GAGAGAGCAA AGCTGAGCGT AGCTGCCAAG CGTTCCCTCT TCAGGGAGCT TGAAAAGACT 
TCAGATGGTT CCGTTTTAAA GACATGGAGT CGTAATCCTG CTGTTGAGCG GAGATTGAGG 
AGAGGTCAAG ATCGATCCCG CACTCAGCCA GTCACCACAG AGGAAGTAGT TATTGCTGCT 
ACTGCCCCTG GCCATTTGTC ACAGTCCGTG ACTTTACAAA CCTCTATAAC GCGCGTTCCT 
AGTCCCACTC TAGTCAGCAC CTCTGTGACC AAACACAGCT TGCAGGCTTC GTCTCAGCAA 
AGCTCCATGG TGAGAGAGCA GGCCAGAGAG GTTCGTCTGG CTCAGGAGGC AACGGAGACA 
GATCGAGCTC GCCACGCTTC TCCGGGGGAA GGCGGACACG AGGAGCCAGA CCTTTCCACA 
CTCACTCTAG CAGAAAAGAT GGCACTTTTT AACCGTCTTG CCCAGCCGTC CAGCCACGGC 
TCTAGGGCCA GAGGAGACAC GCGTGCACGG AGGAGTAACG CACGCTATCA GACTCAACCA 
ATCACACTAG GAGAAGTCGA ACAGGATGGT TCTGGTTCTG AGATGTTCGA TGAGGAATCT 
GAGTTCTCAA TGTGTCTCAG TGATCTGGAA CATTTTCAGC CTGCAGGTGG AGCTCGTTTT 
GCACCCAGCT CCAACTCAGC CATGAGATCC TCCACTGTTT CCACTGAACA TGCGGGCGAC 
ATCCATCTGA CCATGCCAAA TGTATCTGGG CCTGCATATT ATGAGCAGAC CGTTTCTCCA 
CCGCATCACC CTCAGCCTGC CCATGAGATC CAGACACATC AGAGTCTGGC TGAAGGGGAA 
AGGCTGCTGT TGAGCACTGG GAAGAGGAAG CTTCCCTCCC CAGACAGCGT GAAACGGACA 
CCGCATGCTC AGCCGGCCAG AGACTGGGAC AGTAAGGCTG TGGCGGTGAC CTCTCAAGAC 
CTTTCACAAA CAGATGGAGA GCACACAACA GATCTTCCTG TACACACTGC CGCTGCTGTA 
TCTGCATTAC AGAGCCATTC TCTCCATGAG GACCACACCA GAGAGGAAGA GCAGGAGAGC 
GACTTCAAAA CAGAAGACGG AGACGAGCTC TCGGACGTCA TGTCTGCCAG ACAGATGTCC 
ATCAAAGAGA GGCTGGCTCT GCTGAAGAAG AGTGGAGAGG AAGACTGGAG GAACAGGATA 
AATAAAAAAC AGGATGTGGT GAAGGTTGCT GTGTCTGAAA GACATGCCCA GCTATGGGAG 
GTGGAGCAGA GCTTCAAGAA GAAGGATGAT GGGATGATGA TAGATGACTT TGCTGTGTCT 
GATCAACTCT GGGATCCTGT CTTTTCCTCC TCCCTTTTGT GTGCCTCTGA ACCACTGGAT 
CAAATAGCAA ACCTTCCCAA GGCTACCCTC CTGCCCGTGC AGGTGGATGA GCGTCACCCC 
TGGAGACGGA AGAAGATGGG AAATGAAGTT GTGGAAAGCC AACAGCTTGA GCGAGTAGAG 
ATGTCTATTC AGGAACGCAA GCAGCTCATC ACTTCACAAG AGGAGGCCTG GAAAGCCAAA 
GGTTACGGAG CAGCCAACGA CTCCACTCAG TTTACAGTGG CTGGACGTAT GGTCAAAAAA 
GGCCTGGCAG CCCCTTCTGT TCTCACAAAT CCCGTGTTGT CTCCTCTGTC TTCTAAAAAC 
AAGAATACAT CCCATACCAT CACAAAACCA CATGAAGAGA TTGAAGTCAA ACAGAACATG 
AAGCTGGAGT CAGACATGAA GTTAGAAAAG TTGGAAACAT TCCTTGGTCG TTTGAATAGT 
AAAGTTCCAG GACTTCAGGA GGCCACAATT GCTGTCACGG AGAAGTCTGT AAAAGAGGTC 
ATGACTCTGG ACGATGAGGT CTTTGATAAG TTTTATCGAC ATGCAGAGGA TATGGCAAAG 
TTCACCAGCA GAGTGGAAAT TAGTGATGAT TTTGATGACA TCTTTGGAGT ACAGCTGCCC 
AGGTTGACTT CGGAAATGGT GCAGCACAAG CGGGCAGTTC GGCCTACCAG AAATGTTCAG 
TCATCTAAAA ACCCGCTGAA AATGCTGGCT GTTCGGGAGG ACATCAGACA TGAGTACACG 
GAACAAAGAC TGAACATCGG CCTTCTGGAG AGCAAGAGGA TGAAACAGGA AAAGATGAAT 
AAGAACAGCG GTTTCTCTGA GGTTGCTCTC GCCGGATTGG CCAGTAAGGA GAACTTCAGC 
AGTGTGAGTC TGCGAAGTGT GAATGCCTCT GAACAAAGCT CCAACAACAG TGCCATGCCC 
TATAAAAAAC TCATGCTCAT TCAGGTCAAA GGTCGCAGGC ACGTTCAGAC CAGACTGGTT 
GAGCCCAGAG CTTCCTCCTT GAACAGTGGC GACTGCTTCC TCCTGATCAC ACCACATCAT 
TGCTTCATCT GGATTGGAGA ATTCGCTAAT GTTATTGAGA AGGCCAAAGC TGCAGAGTTG 
GCCACGTTCG TTCAAACCAA GCATGACCTG GGATGCAGAG CCTCGTATGT TCAGACAATT 
GAGGAGGGTG CAAACACTCA CACACACGCT GCCAAAGACT TTTGGAAGAT TCTCGGTGGC 
CAAACCAGTT ATCAGTCCGC TGGCACTCCT GAACAAGATG AGTTTTATGA GAGTGCAGTC 
GTAGAAACCA ACTGCATATA CAGACTCATG GAGGATAAAC TTGTTCCCCA TGATGACTAC 
TGGGGTCGAG TCCCTCGCTG CTCCATGCTG AACCCTAAAG AGGTGCTGGT ATTTGATTTT 
GGTAGTGAGG TCTATGTATG GCATGGCAAG GAGGTGACGT TGGCACAGAG AAAAGTGGCA 
TTTCAGCTGG CGAAGCACTT GTGGAATGGC ACCTTTGACT ACACCAACTG TGACATTAAT 
CCTCTGGACC CTGGAGAGTG CAATGCTCTC ATACCCAGGA AAGGCCAGGG ACGACCAGAC 
TGGGCTGTGT TTGGCAGGTT AACTCAGCAC AATGAAACTA CTCTTTTTAA AGAGAAGTTT 
TTGGACTGGA GTGATTCCAA GAATTCTAAG AAAAATGGTG ACTTGCATGT TGAAAACAAG 
GAGCTTCGGA GTGGTGAATG TCGGCCCTAT GATGCGTCTC TGATGCTATC TGCATTACGG 
GCGCCAGTCA GGACCGTTCT GGATGGGGTG AACATTGGAC GAGGATATGG ATTAGTGGAA 
GGTGAAGAGG GACGTAATTT TGAGATCAGC ACATTGTCTG TGGATGTCTG GCATATTTTA 
GAGTTTGACT ACAGTCGACT ACCCAAACAG AGCATTGGTC AGTTTCATGA AGGTGACACA 
TACGTGGTCA AATGGAAGTA CATGATCAGC AGAGCAGTTG GCAAGAGACT GCATTCAGAA 
AGAATCATCG GTCCAGGGAA GGAGAAATGT TGTTATTTCT TCTGGCAGGG TCGTAACTCT 
ACGGTGAATG AAAAGGGAAC GTCAGCACTG ATGACTGTTG AGCTGGATGA GGAAAGAGGA 
GCTCAGGTCC AGGTACAGCA AGGAAAGGAG CCTCCTTGTT TCCTACAGTG CTTCAATGGA 
GGAATGATTG TTCATGCTGG AAAAAGAGAA GAGGAGGAAG ACAATACACA AAACGACTGG 
CGATTGTACT GTGTGAGAGG AGAGAAACCC ATAGAGGGCC ATCTGCTGGA GGCAGTGTGT 
CACTGCAGCA GCTTGCGATC CCGGACCTCT ATGATCCTCC TGAACATCCC CAAAGCCAGC 
CTATACCTGT GGCATGGCTG CAAGGCACAA GTGCACACAC GTGACGTGGG CCGTACCACT 
GCAAACAAAA TCAAAGAGCA ATGTCCTCTG GAGGCAGGGC TTCACAGCAG CAGTAAAGTG 
TCCATCCAGG AGTGTGATGA GGGAGCTGAA CCACAGGGCT TCTGGGAGGC TTTGGGCAAA 
AGAGATCGAA AAGCGTACGA CTGCATGCTG CAGGATCCAG GAAAGTTCAA CTTCACTCCT 
CGGCTTTTCC AGTTAAGCAG TGCCTCGGGA GAGTTTGCAG CAGTGGAGTT TGTATATCCA 
TCCAGAGAAC CAAATTTGGT TAACTCGATG CCTTTCCTCC AGGAGGATTT GTATACAGCT 
ACTCAGCCAG CCCTGTTTTT GGTGGATAAT CATCATGAAG TTTACCTGTG GCAGGGCTGG 
TGGCCTCAGG ACAGTGAAAG CACAGGCTCA GCTCGCATAC GGTGGGACTC TGACAGGAAG 
TGTGCAATGG AAACTGTGCT CCAGTATAGC AAAGAAAAAA ATGAGAAAAA GACACCAAAA 
TCCTACCTGA TCCATGCAGG ACTGGAGCCT CTTACATTCA CCAACATGTT TCCCAGCTGG 
GAACACAGAG AGGACATCGC TGAAATCACA GAGAAGGAGG CCGAAGTATG TCATCAGATC 
ATTCTTGTTG AAGATGTCTT GAGCCGACTC TGCAAGACCA CTTACCCACT GGCTGAACTA 
AAAGCACGTC CCTTACCGGA AGGTGTCGAC CCTCTACGTC TTGAAACCTA CTTATCAGAT 
GAGGACTTTG AGGGTGTAGG GGCTTCTGGG AAGAAAGCTT TGGAGATGAC GAGAAGTGAA 
TATGAGGCAC TGCCTGGATG GAAGCAAGTG AATGTGAAGA AAGCCAAAGG ATTGTTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

svila ( XM_021480014.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021335689.1 Danio rerio supervillin a (svila), transcript variant X4, mRNA.

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CloneID	ODa148132
Clone ID Related Accession (Same CDS sequence)	XM_021480014.1
Accession Version	XM_021480014.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6627bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	supervillin isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007123.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTATCGGA ACCCTTGGAT CTCCAACTAT ATCAACCATG TCAAGTTGTG CCAAGGCTTC 
ACCAGAGAAA CAGCCCCTAA AGTTGGCAAT ACCAAGCAAC GGAGAGCTCT TAGTTTTCAG 
AAAGAGTTCT CGTTTGAGGA GAAAGGTGAG GGAGCCAACA AAGAAGTAGA TGTTAGTCTG 
CTGGGGAATA CACCCAAGGT CTCAGAATTA AGGAAACGTT TTGAAAGTAT AAGCACAAGT 
CAGAGTTGGG ACATGAACAG GAAGGAGCGA ATTGCACGGC GCCTGGAAGG AATCGATAAT 
GATGTTCTCC AAAGTCTTCC AGGTTGTGGT GGTCTAGTTT CTAATCGACT GCTGGAGGAG 
GACACTCCAA GATATACCCG TGCAGCAGAC ACCTGTGACC CCTGTACAGT TTCTCTTCAG 
CACTATGGCA AAAGTGAACC AAGTTCACAA AGCACAGATG TTCCATCCAG AAGTTACGCA 
GTCCACCCGG AGTCTGTCTT CAGCACTGGC TCCTCTCATG TGGCAGAGCT GGACTCTAAA 
GCAGAAAGGA TTGCACGCTA CAAGGCTGAA CGCCGCCGTC AGTTAGCAGA GCGCTATGGC 
ATCTCTCTAG ATCAGGAGAT GGAAACAGAC TATACAACTC GCTTCTCCAG ACCCTCCAGA 
GAGCTTGATG GTTCAGACAG ACACCACAGG TCAAAATCAG AAGGGAGAGA TGGAGTCGAG 
CAAAATGCTT ACACCAGCAG CCATCATGTG GATAAGAGCA GCTCAGACTA TCACTCCAGT 
CCAGAGTACA GCCGCGAGCG CACAGACTCC ATCTCAGAAC GTGAAAGAAT GATGAATCTG 
GAGAACCAGA GGAGGGCACA GGAGCGTGAC CGATTACATG GAGGTGGAGG GGTTGCACCT 
GACCCATCTG CGTATATGGA TGTGTCTGGA TCTGCAAGGG TACCAGGAAG GGAGCCAGGG 
ATGATGGGAG TTCCCAGCTC ACCCAATTCA GGCCGAAGGG GCTTGATGCT CTCTCCCAAA 
CAGGGAGCGT CTCCTGGTGA TCTGTTTATT GAGCAGCAGG CTCACAGCAT CCTCCAAAGA 
CACGGTCAGT CATCCAACCA ATGGTCCCTT CAGACCGATT CAGAAGGAGA CACCGTCTCT 
CCAATCAACT GGCCTTCCAG AATCAGAGTT AGAGAGCGAC TGGCCCGCGA TGAGCCATTT 
CACAGGGGTC AAACAACAGA GGAACCTGCT TACAACCAGT CATATCTTCG TTATTACCCT 
CACTCACAGA GCCTCCTAGA TGGTCCTGTC AAACAGCAGC AACTGACCCG TTCTGAAAAA 
AAGGAACGCC ATGATATGGG TTGCCAGAGT TACTTCTCCA TGGGCAGCGA ATCCACTTCC 
AGGAATAAGC AAGAGATTCA GGAGGTGGTT GAAGAAATGG AGGGAAAACA TGTTGCGAGG 
ACTGAAGGTC TTCTGCGGAG CAGAAAGGCA GTTTTACCTT CTGAGATTCG AAGGAAGGAA 
CGGAACACAG ACGATTCATG CTCTGGAAGT GGTGACAACA GGGAGGTAAG CTCAGCTATT 
GCTGGGCACA GGAGCAGCGA GGGTGCTGCA AATGACCCCA GAGGGTGGCA TGGAAGTCGG 
GCAAGACGGG AAGTTTATAC AGAGCACGTG TCTCGACTTC AGGCTGCAGA GTCTTTGCAG 
GCAAGAGCTC GAGAAGCTCA TAGAAGGCCT GATGCTAGCT GCTGCGCCCC AATCCAGACC 
TCCAGTGCAA GACCAATGAC AAATTCATCT AAACCCATGG ATACTGGAAA TACACAAAAA 
ACTGTTCTCC CCATCCAGGA GCCAATCAGA GAGGCTGTCC AACAAGAAAA AGTGCCAAAT 
GTTGATCACC TGTCCCATGA TGCCAGGGTT TCAGTAGCCC ATCTCCGCCA CACATACCTG 
CAAAGTGTTG CAAGTCCCCG GAAAGCCGAA CTGTCTCATC TAAGCTCTGA ACCATCAAGC 
CCTGAGGTGG ATGAAGAGAA ACTAGATGAG AGAGCAAAGC TGAGCGTAGC TGCCAAGCGT 
TCCCTCTTCA GGGAGCTTGA AAAGACTTCA GATGGTTCCG TTTTAAAGAC ATGGAGTCGT 
AATCCTGCTG TTGAGCGGAG ATTGAGGAGA GGTCAAGATC GATCCCGCAC TCAGCCAGTC 
ACCACAGAGG AAGTAGTTAT TGCTGCTACT GCCCCTGGCC ATTTGTCACA GTCCGTGACT 
TTACAAACCT CTATAACGCG CGTTCCTAGT CCCACTCTAG TCAGCACCTC TGTGACCAAA 
CACAGCTTGC AGGCTTCGTC TCAGCAAAGC TCCATGGTGA GAGAGCAGGC CAGAGAGGTT 
CGTCTGGCTC AGGAGGCAAC GGAGACAGAT CGAGCTCGCC ACGCTTCTCC GGGGGAAGGC 
GGACACGAGG AGCCAGACCT TTCCACACTC ACTCTAGCAG AAAAGATGGC ACTTTTTAAC 
CGTCTTGCCC AGCCGTCCAG CCACGGCTCT AGGGCCAGAG GAGACACGCG TGCACGGAGG 
AGTAACGCAC GCTATCAGAC TCAACCAATC ACACTAGGAG AAGTCGAACA GGATGGTTCT 
GGTTCTGAGA TGTTCGATGA GGAATCTGAG TTCTCAATGT GTCTCAGTGA TCTGGAACAT 
TTTCAGCCTG CAGGTGGAGC TCGTTTTGCA CCCAGCTCCA ACTCAGCCAT GAGATCCTCC 
ACTGTTTCCA CTGAACATGC GGGCGACATC CATCTGACCA TGCCAAATGT ATCTGGGCCT 
GCATATTATG AGCAGACCGT TTCTCCACCG CATCACCCTC AGCCTGCCCA TGAGATCCAG 
ACACATCAGA GTCTGGCTGA AGGGGAAAGG CTGCTGTTGA GCACTGGGAA GAGGAAGCTT 
CCCTCCCCAG ACAGCGTGAA ACGGACACCG CATGCTCAGC CGGCCAGAGA CTGGGACAGT 
AAGGCTGTGG CGGTGACCTC TCAAGACCTT TCACAAACAG ATGGAGAGCA CACAACAGAT 
CTTCCTGTAC ACACTGCCGC TGCTGTATCT GCATTACAGA GCCATTCTCT CCATGAGGAC 
CACACCAGAG AGGAAGAGCA GGAGAGCGAC TTCAAAACAG AAGACGGAGA CGAGCTCTCG 
GACGTCATGT CTGCCAGACA GATGTCCATC AAAGAGAGGC TGGCTCTGCT GAAGAAGAGT 
GGAGAGGAAG ACTGGAGGAA CAGGATAAAT AAAAAACAGG ATGTGGTGAA GGTTGCTGTG 
TCTGAAAGAC ATGCCCAGCT ATGGGAGGTG GAGCAGAGCT TCAAGAAGAA GGATGATGGG 
ATGATGATAG ATGACTTTGC TGTGTCTGAT CAACTCTGGG ATCCTGTCTT TTCCTCCTCC 
CTTTTGTGTG CCTCTGAACC ACTGGATCAA ATAGCAAACC TTCCCAAGGC TACCCTCCTG 
CCCGTGCAGG TGGATGAGCG TCACCCCTGG AGACGGAAGA AGATGGGAAA TGAAGTTGTG 
GAAAGCCAAC AGCTTGAGCG AGTAGAGATG TCTATTCAGG AACGCAAGCA GCTCATCACT 
TCACAAGAGG AGGCCTGGAA AGCCAAAGGT TACGGAGCAG CCAACGACTC CACTCAGTTT 
ACAGTGGCTG GACGTATGGT CAAAAAAGGC CTGGCAGCCC CTTCTGTTCT CACAAATCCC 
GTGTTGTCTC CTCTGTCTTC TAAAAACAAG AATACATCCC ATACCATCAC AAAACCACAT 
GAAGAGATTG AAGTCAAACA GAACATGAAG CTGGAGTCAG ACATGAAGTT AGAAAAGTTG 
GAAACATTCC TTGGTCGTTT GAATAGTAAA GTTCCAGGAC TTCAGGAGGC CACAATTGCT 
GTCACGGAGA AGTCTGTAAA AGAGGTCATG ACTCTGGACG ATGAGGTCTT TGATAAGTTT 
TATCGACATG CAGAGGATAT GGCAAAGTTC ACCAGCAGAG TGGAAATTAG TGATGATTTT 
GATGACATCT TTGGAGTACA GCTGCCCAGG TTGACTTCGG AAATGGTGCA GCACAAGCGG 
GCAGTTCGGC CTACCAGAAA TGTTCAGTCA TCTAAAAACC CGCTGAAAAT GCTGGCTGTT 
CGGGAGGACA TCAGACATGA GTACACGGAA CAAAGACTGA ACATCGGCCT TCTGGAGAGC 
AAGAGGATGA AACAGGAAAA GATGAATAAG AACAGCGGTT TCTCTGAGGT TGCTCTCGCC 
GGATTGGCCA GTAAGGAGAA CTTCAGCAGT GTGAGTCTGC GAAGTGTGAA TGCCTCTGAA 
CAAAGCTCCA ACAACAGTGC CATGCCCTAT AAAAAACTCA TGCTCATTCA GGTCAAAGGT 
CGCAGGCACG TTCAGACCAG ACTGGTTGAG CCCAGAGCTT CCTCCTTGAA CAGTGGCGAC 
TGCTTCCTCC TGATCACACC ACATCATTGC TTCATCTGGA TTGGAGAATT CGCTAATGTT 
ATTGAGAAGG CCAAAGCTGC AGAGTTGGCC ACGTTCGTTC AAACCAAGCA TGACCTGGGA 
TGCAGAGCCT CGTATGTTCA GACAATTGAG GAGGGTGCAA ACACTCACAC ACACGCTGCC 
AAAGACTTTT GGAAGATTCT CGGTGGCCAA ACCAGTTATC AGTCCGCTGG CACTCCTGAA 
CAAGATGAGT TTTATGAGAG TGCAGTCGTA GAAACCAACT GCATATACAG ACTCATGGAG 
GATAAACTTG TTCCCCATGA TGACTACTGG GGTCGAGTCC CTCGCTGCTC CATGCTGAAC 
CCTAAAGAGG TGCTGGTATT TGATTTTGGT AGTGAGGTCT ATGTATGGCA TGGCAAGGAG 
GTGACGTTGG CACAGAGAAA AGTGGCATTT CAGCTGGCGA AGCACTTGTG GAATGGCACC 
TTTGACTACA CCAACTGTGA CATTAATCCT CTGGACCCTG GAGAGTGCAA TGCTCTCATA 
CCCAGGAAAG GCCAGGGACG ACCAGACTGG GCTGTGTTTG GCAGGTTAAC TCAGCACAAT 
GAAACTACTC TTTTTAAAGA GAAGTTTTTG GACTGGAGTG ATTCCAAGAA TTCTAAGAAA 
AATGGTGACT TGCATGTTGA AAACAAGGAG CTTCGGAGTG GTGAATGTCG GCCCTATGAT 
GCGTCTCTGA TGCTATCTGC ATTACGGGCG CCAGTCAGGA CCGTTCTGGA TGGGGTGAAC 
ATTGGACGAG GATATGGATT AGTGGAAGGT GAAGAGGGAC GTAATTTTGA GATCAGCACA 
TTGTCTGTGG ATGTCTGGCA TATTTTAGAG TTTGACTACA GTCGACTACC CAAACAGAGC 
ATTGGTCAGT TTCATGAAGG TGACACATAC GTGGTCAAAT GGAAGTACAT GATCAGCAGA 
GCAGTTGGCA AGAGACTGCA TTCAGAAAGA ATCATCGGTC CAGGGAAGGA GAAATGTTGT 
TATTTCTTCT GGCAGGGTCG TAACTCTACG GTGAATGAAA AGGGAACGTC AGCACTGATG 
ACTGTTGAGC TGGATGAGGA AAGAGGAGCT CAGGTCCAGG TACAGCAAGG AAAGGAGCCT 
CCTTGTTTCC TACAGTGCTT CAATGGAGGA ATGATTGTTC ATGCTGGAAA AAGAGAAGAG 
GAGGAAGACA ATACACAAAA CGACTGGCGA TTGTACTGTG TGAGAGGAGA GAAACCCATA 
GAGGGCCATC TGCTGGAGGC AGTGTGTCAC TGCAGCAGCT TGCGATCCCG GACCTCTATG 
ATCCTCCTGA ACATCCCCAA AGCCAGCCTA TACCTGTGGC ATGGCTGCAA GGCACAAGTG 
CACACACGTG ACGTGGGCCG TACCACTGCA AACAAAATCA AAGAGCAATG TCCTCTGGAG 
GCAGGGCTTC ACAGCAGCAG TAAAGTGTCC ATCCAGGAGT GTGATGAGGG AGCTGAACCA 
CAGGGCTTCT GGGAGGCTTT GGGCAAAAGA GATCGAAAAG CGTACGACTG CATGCTGCAG 
GATCCAGGAA AGTTCAACTT CACTCCTCGG CTTTTCCAGT TAAGCAGTGC CTCGGGAGAG 
TTTGCAGCAG TGGAGTTTGT ATATCCATCC AGAGAACCAA ATTTGGTTAA CTCGATGCCT 
TTCCTCCAGG AGGATTTGTA TACAGCTACT CAGCCAGCCC TGTTTTTGGT GGATAATCAT 
CATGAAGTTT ACCTGTGGCA GGGCTGGTGG CCTCAGGACA GTGAAAGCAC AGGCTCAGCT 
CGCATACGGT GGGACTCTGA CAGGAAGTGT GCAATGGAAA CTGTGCTCCA GTATAGCAAA 
GAAAAAAATG AGAAAAAGAC ACCAAAATCC TACCTGATCC ATGCAGGACT GGAGCCTCTT 
ACATTCACCA ACATGTTTCC CAGCTGGGAA CACAGAGAGG ACATCGCTGA AATCACAGAG 
AAGGAGGCCG AAGTATGTCA TCAGATCATT CTTGTTGAAG ATGTCTTGAG CCGACTCTGC 
AAGACCACTT ACCCACTGGC TGAACTAAAA GCACGTCCCT TACCGGAAGG TGTCGACCCT 
CTACGTCTTG AAACCTACTT ATCAGATGAG GACTTTGAGG GTGTAGGGGC TTCTGGGAAG 
AAAGCTTTGG AGATGACGAG AAGTGAATAT GAGGCACTGC CTGGATGGAA GCAAGTGAAT 
GTGAAGAAAG CCAAAGGATT GTTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021335689.1
CDS	343..6969
Translation	MYRNPWISNYINHVKLCQGFTRETAPKVGNTKQRRALSFQKEFSFEEKGEGANKEVDVSLLGNTPKVSELRKRFESISTSQSWDMNRKERIARRLEGIDNDVLQSLPGCGGLVSNRLLEEDTPRYTRAADTCDPCTVSLQHYGKSEPSSQSTDVPSRSYAVHPESVFSTGSSHVAELDSKAERIARYKAERRRQLAERYGISLDQEMETDYTTRFSRPSRELDGSDRHHRSKSEGRDGVEQNAYTSSHHVDKSSSDYHSSPEYSRERTDSISERERMMNLENQRRAQERDRLHGGGGVAPDPSAYMDVSGSARVPGREPGMMGVPSSPNSGRRGLMLSPKQGASPGDLFIEQQAHSILQRHGQSSNQWSLQTDSEGDTVSPINWPSRIRVRERLARDEPFHRGQTTEEPAYNQSYLRYYPHSQSLLDGPVKQQQLTRSEKKERHDMGCQSYFSMGSESTSRNKQEIQEVVEEMEGKHVARTEGLLRSRKAVLPSEIRRKERNTDDSCSGSGDNREVSSAIAGHRSSEGAANDPRGWHGSRARREVYTEHVSRLQAAESLQARAREAHRRPDASCCAPIQTSSARPMTNSSKPMDTGNTQKTVLPIQEPIREAVQQEKVPNVDHLSHDARVSVAHLRHTYLQSVASPRKAELSHLSSEPSSPEVDEEKLDERAKLSVAAKRSLFRELEKTSDGSVLKTWSRNPAVERRLRRGQDRSRTQPVTTEEVVIAATAPGHLSQSVTLQTSITRVPSPTLVSTSVTKHSLQASSQQSSMVREQAREVRLAQEATETDRARHASPGEGGHEEPDLSTLTLAEKMALFNRLAQPSSHGSRARGDTRARRSNARYQTQPITLGEVEQDGSGSEMFDEESEFSMCLSDLEHFQPAGGARFAPSSNSAMRSSTVSTEHAGDIHLTMPNVSGPAYYEQTVSPPHHPQPAHEIQTHQSLAEGERLLLSTGKRKLPSPDSVKRTPHAQPARDWDSKAVAVTSQDLSQTDGEHTTDLPVHTAAAVSALQSHSLHEDHTREEEQESDFKTEDGDELSDVMSARQMSIKERLALLKKSGEEDWRNRINKKQDVVKVAVSERHAQLWEVEQSFKKKDDGMMIDDFAVSDQLWDPVFSSSLLCASEPLDQIANLPKATLLPVQVDERHPWRRKKMGNEVVESQQLERVEMSIQERKQLITSQEEAWKAKGYGAANDSTQFTVAGRMVKKGLAAPSVLTNPVLSPLSSKNKNTSHTITKPHEEIEVKQNMKLESDMKLEKLETFLGRLNSKVPGLQEATIAVTEKSVKEVMTLDDEVFDKFYRHAEDMAKFTSRVEISDDFDDIFGVQLPRLTSEMVQHKRAVRPTRNVQSSKNPLKMLAVREDIRHEYTEQRLNIGLLESKRMKQEKMNKNSGFSEVALAGLASKENFSSVSLRSVNASEQSSNNSAMPYKKLMLIQVKGRRHVQTRLVEPRASSLNSGDCFLLITPHHCFIWIGEFANVIEKAKAAELATFVQTKHDLGCRASYVQTIEEGANTHTHAAKDFWKILGGQTSYQSAGTPEQDEFYESAVVETNCIYRLMEDKLVPHDDYWGRVPRCSMLNPKEVLVFDFGSEVYVWHGKEVTLAQRKVAFQLAKHLWNGTFDYTNCDINPLDPGECNALIPRKGQGRPDWAVFGRLTQHNETTLFKEKFLDWSDSKNSKKNGDLHVENKELRSGECRPYDASLMLSALRAPVRTVLDGVNIGRGYGLVEGEEGRNFEISTLSVDVWHILEFDYSRLPKQSIGQFHEGDTYVVKWKYMISRAVGKRLHSERIIGPGKEKCCYFFWQGRNSTVNEKGTSALMTVELDEERGAQVQVQQGKEPPCFLQCFNGGMIVHAGKREEEEDNTQNDWRLYCVRGEKPIEGHLLEAVCHCSSLRSRTSMILLNIPKASLYLWHGCKAQVHTRDVGRTTANKIKEQCPLEAGLHSSSKVSIQECDEGAEPQGFWEALGKRDRKAYDCMLQDPGKFNFTPRLFQLSSASGEFAAVEFVYPSREPNLVNSMPFLQEDLYTATQPALFLVDNHHEVYLWQGWWPQDSESTGSARIRWDSDRKCAMETVLQYSKEKNEKKTPKSYLIHAGLEPLTFTNMFPSWEHREDIAEITEKEAEVCHQIILVEDVLSRLCKTTYPLAELKARPLPEGVDPLRLETYLSDEDFEGVGASGKKALEMTRSEYEALPGWKQVNVKKAKGLF

Target ORF information:

RefSeq Version	XM_021480014.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio supervillin a (svila), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021480014.1

ORF Insert Sequence:

ATGTATCGGA ACCCTTGGAT CTCCAACTAT ATCAACCATG TCAAGTTGTG CCAAGGCTTC 
ACCAGAGAAA CAGCCCCTAA AGTTGGCAAT ACCAAGCAAC GGAGAGCTCT TAGTTTTCAG 
AAAGAGTTCT CGTTTGAGGA GAAAGGTGAG GGAGCCAACA AAGAAGTAGA TGTTAGTCTG 
CTGGGGAATA CACCCAAGGT CTCAGAATTA AGGAAACGTT TTGAAAGTAT AAGCACAAGT 
CAGAGTTGGG ACATGAACAG GAAGGAGCGA ATTGCACGGC GCCTGGAAGG AATCGATAAT 
GATGTTCTCC AAAGTCTTCC AGGTTGTGGT GGTCTAGTTT CTAATCGACT GCTGGAGGAG 
GACACTCCAA GATATACCCG TGCAGCAGAC ACCTGTGACC CCTGTACAGT TTCTCTTCAG 
CACTATGGCA AAAGTGAACC AAGTTCACAA AGCACAGATG TTCCATCCAG AAGTTACGCA 
GTCCACCCGG AGTCTGTCTT CAGCACTGGC TCCTCTCATG TGGCAGAGCT GGACTCTAAA 
GCAGAAAGGA TTGCACGCTA CAAGGCTGAA CGCCGCCGTC AGTTAGCAGA GCGCTATGGC 
ATCTCTCTAG ATCAGGAGAT GGAAACAGAC TATACAACTC GCTTCTCCAG ACCCTCCAGA 
GAGCTTGATG GTTCAGACAG ACACCACAGG TCAAAATCAG AAGGGAGAGA TGGAGTCGAG 
CAAAATGCTT ACACCAGCAG CCATCATGTG GATAAGAGCA GCTCAGACTA TCACTCCAGT 
CCAGAGTACA GCCGCGAGCG CACAGACTCC ATCTCAGAAC GTGAAAGAAT GATGAATCTG 
GAGAACCAGA GGAGGGCACA GGAGCGTGAC CGATTACATG GAGGTGGAGG GGTTGCACCT 
GACCCATCTG CGTATATGGA TGTGTCTGGA TCTGCAAGGG TACCAGGAAG GGAGCCAGGG 
ATGATGGGAG TTCCCAGCTC ACCCAATTCA GGCCGAAGGG GCTTGATGCT CTCTCCCAAA 
CAGGGAGCGT CTCCTGGTGA TCTGTTTATT GAGCAGCAGG CTCACAGCAT CCTCCAAAGA 
CACGGTCAGT CATCCAACCA ATGGTCCCTT CAGACCGATT CAGAAGGAGA CACCGTCTCT 
CCAATCAACT GGCCTTCCAG AATCAGAGTT AGAGAGCGAC TGGCCCGCGA TGAGCCATTT 
CACAGGGGTC AAACAACAGA GGAACCTGCT TACAACCAGT CATATCTTCG TTATTACCCT 
CACTCACAGA GCCTCCTAGA TGGTCCTGTC AAACAGCAGC AACTGACCCG TTCTGAAAAA 
AAGGAACGCC ATGATATGGG TTGCCAGAGT TACTTCTCCA TGGGCAGCGA ATCCACTTCC 
AGGAATAAGC AAGAGATTCA GGAGGTGGTT GAAGAAATGG AGGGAAAACA TGTTGCGAGG 
ACTGAAGGTC TTCTGCGGAG CAGAAAGGCA GTTTTACCTT CTGAGATTCG AAGGAAGGAA 
CGGAACACAG ACGATTCATG CTCTGGAAGT GGTGACAACA GGGAGGTAAG CTCAGCTATT 
GCTGGGCACA GGAGCAGCGA GGGTGCTGCA AATGACCCCA GAGGGTGGCA TGGAAGTCGG 
GCAAGACGGG AAGTTTATAC AGAGCACGTG TCTCGACTTC AGGCTGCAGA GTCTTTGCAG 
GCAAGAGCTC GAGAAGCTCA TAGAAGGCCT GATGCTAGCT GCTGCGCCCC AATCCAGACC 
TCCAGTGCAA GACCAATGAC AAATTCATCT AAACCCATGG ATACTGGAAA TACACAAAAA 
ACTGTTCTCC CCATCCAGGA GCCAATCAGA GAGGCTGTCC AACAAGAAAA AGTGCCAAAT 
GTTGATCACC TGTCCCATGA TGCCAGGGTT TCAGTAGCCC ATCTCCGCCA CACATACCTG 
CAAAGTGTTG CAAGTCCCCG GAAAGCCGAA CTGTCTCATC TAAGCTCTGA ACCATCAAGC 
CCTGAGGTGG ATGAAGAGAA ACTAGATGAG AGAGCAAAGC TGAGCGTAGC TGCCAAGCGT 
TCCCTCTTCA GGGAGCTTGA AAAGACTTCA GATGGTTCCG TTTTAAAGAC ATGGAGTCGT 
AATCCTGCTG TTGAGCGGAG ATTGAGGAGA GGTCAAGATC GATCCCGCAC TCAGCCAGTC 
ACCACAGAGG AAGTAGTTAT TGCTGCTACT GCCCCTGGCC ATTTGTCACA GTCCGTGACT 
TTACAAACCT CTATAACGCG CGTTCCTAGT CCCACTCTAG TCAGCACCTC TGTGACCAAA 
CACAGCTTGC AGGCTTCGTC TCAGCAAAGC TCCATGGTGA GAGAGCAGGC CAGAGAGGTT 
CGTCTGGCTC AGGAGGCAAC GGAGACAGAT CGAGCTCGCC ACGCTTCTCC GGGGGAAGGC 
GGACACGAGG AGCCAGACCT TTCCACACTC ACTCTAGCAG AAAAGATGGC ACTTTTTAAC 
CGTCTTGCCC AGCCGTCCAG CCACGGCTCT AGGGCCAGAG GAGACACGCG TGCACGGAGG 
AGTAACGCAC GCTATCAGAC TCAACCAATC ACACTAGGAG AAGTCGAACA GGATGGTTCT 
GGTTCTGAGA TGTTCGATGA GGAATCTGAG TTCTCAATGT GTCTCAGTGA TCTGGAACAT 
TTTCAGCCTG CAGGTGGAGC TCGTTTTGCA CCCAGCTCCA ACTCAGCCAT GAGATCCTCC 
ACTGTTTCCA CTGAACATGC GGGCGACATC CATCTGACCA TGCCAAATGT ATCTGGGCCT 
GCATATTATG AGCAGACCGT TTCTCCACCG CATCACCCTC AGCCTGCCCA TGAGATCCAG 
ACACATCAGA GTCTGGCTGA AGGGGAAAGG CTGCTGTTGA GCACTGGGAA GAGGAAGCTT 
CCCTCCCCAG ACAGCGTGAA ACGGACACCG CATGCTCAGC CGGCCAGAGA CTGGGACAGT 
AAGGCTGTGG CGGTGACCTC TCAAGACCTT TCACAAACAG ATGGAGAGCA CACAACAGAT 
CTTCCTGTAC ACACTGCCGC TGCTGTATCT GCATTACAGA GCCATTCTCT CCATGAGGAC 
CACACCAGAG AGGAAGAGCA GGAGAGCGAC TTCAAAACAG AAGACGGAGA CGAGCTCTCG 
GACGTCATGT CTGCCAGACA GATGTCCATC AAAGAGAGGC TGGCTCTGCT GAAGAAGAGT 
GGAGAGGAAG ACTGGAGGAA CAGGATAAAT AAAAAACAGG ATGTGGTGAA GGTTGCTGTG 
TCTGAAAGAC ATGCCCAGCT ATGGGAGGTG GAGCAGAGCT TCAAGAAGAA GGATGATGGG 
ATGATGATAG ATGACTTTGC TGTGTCTGAT CAACTCTGGG ATCCTGTCTT TTCCTCCTCC 
CTTTTGTGTG CCTCTGAACC ACTGGATCAA ATAGCAAACC TTCCCAAGGC TACCCTCCTG 
CCCGTGCAGG TGGATGAGCG TCACCCCTGG AGACGGAAGA AGATGGGAAA TGAAGTTGTG 
GAAAGCCAAC AGCTTGAGCG AGTAGAGATG TCTATTCAGG AACGCAAGCA GCTCATCACT 
TCACAAGAGG AGGCCTGGAA AGCCAAAGGT TACGGAGCAG CCAACGACTC CACTCAGTTT 
ACAGTGGCTG GACGTATGGT CAAAAAAGGC CTGGCAGCCC CTTCTGTTCT CACAAATCCC 
GTGTTGTCTC CTCTGTCTTC TAAAAACAAG AATACATCCC ATACCATCAC AAAACCACAT 
GAAGAGATTG AAGTCAAACA GAACATGAAG CTGGAGTCAG ACATGAAGTT AGAAAAGTTG 
GAAACATTCC TTGGTCGTTT GAATAGTAAA GTTCCAGGAC TTCAGGAGGC CACAATTGCT 
GTCACGGAGA AGTCTGTAAA AGAGGTCATG ACTCTGGACG ATGAGGTCTT TGATAAGTTT 
TATCGACATG CAGAGGATAT GGCAAAGTTC ACCAGCAGAG TGGAAATTAG TGATGATTTT 
GATGACATCT TTGGAGTACA GCTGCCCAGG TTGACTTCGG AAATGGTGCA GCACAAGCGG 
GCAGTTCGGC CTACCAGAAA TGTTCAGTCA TCTAAAAACC CGCTGAAAAT GCTGGCTGTT 
CGGGAGGACA TCAGACATGA GTACACGGAA CAAAGACTGA ACATCGGCCT TCTGGAGAGC 
AAGAGGATGA AACAGGAAAA GATGAATAAG AACAGCGGTT TCTCTGAGGT TGCTCTCGCC 
GGATTGGCCA GTAAGGAGAA CTTCAGCAGT GTGAGTCTGC GAAGTGTGAA TGCCTCTGAA 
CAAAGCTCCA ACAACAGTGC CATGCCCTAT AAAAAACTCA TGCTCATTCA GGTCAAAGGT 
CGCAGGCACG TTCAGACCAG ACTGGTTGAG CCCAGAGCTT CCTCCTTGAA CAGTGGCGAC 
TGCTTCCTCC TGATCACACC ACATCATTGC TTCATCTGGA TTGGAGAATT CGCTAATGTT 
ATTGAGAAGG CCAAAGCTGC AGAGTTGGCC ACGTTCGTTC AAACCAAGCA TGACCTGGGA 
TGCAGAGCCT CGTATGTTCA GACAATTGAG GAGGGTGCAA ACACTCACAC ACACGCTGCC 
AAAGACTTTT GGAAGATTCT CGGTGGCCAA ACCAGTTATC AGTCCGCTGG CACTCCTGAA 
CAAGATGAGT TTTATGAGAG TGCAGTCGTA GAAACCAACT GCATATACAG ACTCATGGAG 
GATAAACTTG TTCCCCATGA TGACTACTGG GGTCGAGTCC CTCGCTGCTC CATGCTGAAC 
CCTAAAGAGG TGCTGGTATT TGATTTTGGT AGTGAGGTCT ATGTATGGCA TGGCAAGGAG 
GTGACGTTGG CACAGAGAAA AGTGGCATTT CAGCTGGCGA AGCACTTGTG GAATGGCACC 
TTTGACTACA CCAACTGTGA CATTAATCCT CTGGACCCTG GAGAGTGCAA TGCTCTCATA 
CCCAGGAAAG GCCAGGGACG ACCAGACTGG GCTGTGTTTG GCAGGTTAAC TCAGCACAAT 
GAAACTACTC TTTTTAAAGA GAAGTTTTTG GACTGGAGTG ATTCCAAGAA TTCTAAGAAA 
AATGGTGACT TGCATGTTGA AAACAAGGAG CTTCGGAGTG GTGAATGTCG GCCCTATGAT 
GCGTCTCTGA TGCTATCTGC ATTACGGGCG CCAGTCAGGA CCGTTCTGGA TGGGGTGAAC 
ATTGGACGAG GATATGGATT AGTGGAAGGT GAAGAGGGAC GTAATTTTGA GATCAGCACA 
TTGTCTGTGG ATGTCTGGCA TATTTTAGAG TTTGACTACA GTCGACTACC CAAACAGAGC 
ATTGGTCAGT TTCATGAAGG TGACACATAC GTGGTCAAAT GGAAGTACAT GATCAGCAGA 
GCAGTTGGCA AGAGACTGCA TTCAGAAAGA ATCATCGGTC CAGGGAAGGA GAAATGTTGT 
TATTTCTTCT GGCAGGGTCG TAACTCTACG GTGAATGAAA AGGGAACGTC AGCACTGATG 
ACTGTTGAGC TGGATGAGGA AAGAGGAGCT CAGGTCCAGG TACAGCAAGG AAAGGAGCCT 
CCTTGTTTCC TACAGTGCTT CAATGGAGGA ATGATTGTTC ATGCTGGAAA AAGAGAAGAG 
GAGGAAGACA ATACACAAAA CGACTGGCGA TTGTACTGTG TGAGAGGAGA GAAACCCATA 
GAGGGCCATC TGCTGGAGGC AGTGTGTCAC TGCAGCAGCT TGCGATCCCG GACCTCTATG 
ATCCTCCTGA ACATCCCCAA AGCCAGCCTA TACCTGTGGC ATGGCTGCAA GGCACAAGTG 
CACACACGTG ACGTGGGCCG TACCACTGCA AACAAAATCA AAGAGCAATG TCCTCTGGAG 
GCAGGGCTTC ACAGCAGCAG TAAAGTGTCC ATCCAGGAGT GTGATGAGGG AGCTGAACCA 
CAGGGCTTCT GGGAGGCTTT GGGCAAAAGA GATCGAAAAG CGTACGACTG CATGCTGCAG 
GATCCAGGAA AGTTCAACTT CACTCCTCGG CTTTTCCAGT TAAGCAGTGC CTCGGGAGAG 
TTTGCAGCAG TGGAGTTTGT ATATCCATCC AGAGAACCAA ATTTGGTTAA CTCGATGCCT 
TTCCTCCAGG AGGATTTGTA TACAGCTACT CAGCCAGCCC TGTTTTTGGT GGATAATCAT 
CATGAAGTTT ACCTGTGGCA GGGCTGGTGG CCTCAGGACA GTGAAAGCAC AGGCTCAGCT 
CGCATACGGT GGGACTCTGA CAGGAAGTGT GCAATGGAAA CTGTGCTCCA GTATAGCAAA 
GAAAAAAATG AGAAAAAGAC ACCAAAATCC TACCTGATCC ATGCAGGACT GGAGCCTCTT 
ACATTCACCA ACATGTTTCC CAGCTGGGAA CACAGAGAGG ACATCGCTGA AATCACAGAG 
AAGGAGGCCG AAGTATGTCA TCAGATCATT CTTGTTGAAG ATGTCTTGAG CCGACTCTGC 
AAGACCACTT ACCCACTGGC TGAACTAAAA GCACGTCCCT TACCGGAAGG TGTCGACCCT 
CTACGTCTTG AAACCTACTT ATCAGATGAG GACTTTGAGG GTGTAGGGGC TTCTGGGAAG 
AAAGCTTTGG AGATGACGAG AAGTGAATAT GAGGCACTGC CTGGATGGAA GCAAGTGAAT 
GTGAAGAAAG CCAAAGGATT GTTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

svila ( XM_017358423.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_017213912.1 Danio rerio supervillin a (svila), transcript variant X1, mRNA.

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CloneID	ODa54437
Clone ID Related Accession (Same CDS sequence)	XM_017358423.1
Accession Version	XM_017358423.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6651bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	supervillin isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007123.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTATCGGA ACCCTTGGAT CTCCAACTAT ATCAACCATG TCAAGTTGTG CCAAGGCTTC 
ACCAGAGAAA CAGCCCCTAA AGTTGGCAAT ACCAAGCAAC GGAGAGCTCT TAGTTTTCAG 
AAAGAGTTCT CGTTTGAGGA GAAAGGTGAG GGAGCCAACA AAGAAGTAGA TGTTAGTCTG 
CTGGGGAATA CACCCAAGGT CTCAGAATTA AGGAAACGTT TTGAAAGTAT AAGCACAAGT 
CAGAGTTGGG ACATGAACAG GAAGGAGCGA ATTGCACGGC GCCTGGAAGG AATCGATAAT 
GATGTTCTCC AAAGTCTTCC AGGTTGTGGT GGTCTAGTTT CTAATCGACT GCTGGAGGAG 
GACACTCCAA GATATACCCG TGCAGCAGAC ACCTGTGACC CCTGTACAGT TTCTCTTCAG 
CACTATGGCA AAAGTGAACC AAGTTCACAA AGCACAGATG TTCCATCCAG AAGTTACGCA 
GTCCACCCGG AGTCTGTCTT CAGCACTGGC TCCTCTCATG TGGCAGAGCT GGACTCTAAA 
GCAGAAAGGA TTGCACGCTA CAAGGCTGAA CGCCGCCGTC AGTTAGCAGA GCGCTATGGC 
ATCTCTCTAG ATCAGGAGAT GGAAACAGAC TATACAACTC GCTTCTCCAG ACCCTCCAGA 
GAGCTTGATG GTTCAGACAG ACACCACAGG TCAAAATCAG AAGGGAGAGA TGGAGTCGAG 
CAAAATGCTT ACACCAGCAG CCATCATGTG GATAAGAGCA GCTCAGACTA TCACTCCAGT 
CCAGAGTACA GCCGCGAGCG CACAGACTCC ATCTCAGAAC GTGAAAGAAT GATGAATCTG 
GAGAACCAGA GGAGGGCACA GGAGCGTGAC CGATTACATG GAGGTGGAGG GGTTGCACCT 
GACCCATCTG CGTATATGGA TGTGTCTGGA TCTGCAAGGG TACCAGGAAG GGAGCCAGGG 
ATGATGGGAG TTCCCAGCTC ACCCAATTCA GGCCGAAGGG GCTTGATGCT CTCTCCCAAA 
CAGGGAGCGT CTCCTGGTGA TCTGTTTATT GAGCAGCAGG CTCACAGCAT CCTCCAAAGA 
CACGGAATCA GAGTTAGAGA GCGACTGGCC CGCGATGAGC CATTTCACAG GGGTCAAACA 
ACAGAGGAAC CTGCTTACAA CCAGTCATAT CTTCGTTATT ACCCTCACTC ACAGAGCCTC 
CTAGATGGTC CTGTCAAACA GCAGCAACTG ACCCGTTCTG AAAAAAAGGA ACGCCATGAT 
ATGGGTTGCC AGAGTTACTT CTCCATGGGC AGCGAATCCA CTTCCAGGAA TAAGCAAGAG 
ATTCAGGAGG TGGTTGAAGA AATGGAGGGA AAACATGTTG CGAGGACTGA AGGTCTTCTG 
CGGAGCAGAA AGGCAGTTTT ACCTTCTGAG ATTCGAAGGA AGGAACGGAA CACAGACGAT 
TCATGCTCTG GAAGTGGTGA CAACAGGGAG GTAAGCTCAG CTATTGCTGG GCACAGGAGC 
AGCGAGGGTG CTGCAAATGA CCCCAGAGGG TGGCATGGAA GTCGGGCAAG ACGGGAAGTT 
TATACAGAGC ACGTGTCTCG ACTTCAGGCT GCAGAGTCTT TGCAGGCAAG AGCTCGAGAA 
GCTCATAGAA GGCCTGATGC TAGCTGCTGC GCCCCAATCC AGACCTCCAG TGCAAGACCA 
ATGACAAATT CATCTAAACC CATGGATACT GGAAATACAC AAAAAACTGT TCTCCCCATC 
CAGGAGCCAA TCAGAGAGGC TGTCCAACAA GAAAAAGTGC CAAATGTTGA TCACCTGTCC 
CATGATGCCA GGGTTTCAGT AGCCCATCTC CGCCACACAT ACCTGCAAAG TGTTGCAAGT 
CCCCGGAAAG CCGAACTGGC AGAAGAGCTG CCTGCAGTTA CAGATGTACC CTGGAGAAGC 
ATGCGAGTCA GAGATCGGTC ATCTCGTCGC CCTTTGCGAT ATATATGTCC CACTGAAAGT 
AGAAAGACAT CTGAGAGGTT TAGAACACAG CCAATCACAT CAACCGAGCG TGAGCAGTCG 
GACCGGTCTC ATCTAAGCTC TGAACCATCA AGCCCTGAGG TGGATGAAGA GAAACTAGAT 
GAGAGAGCAA AGCTGAGCGT AGCTGCCAAG CGTTCCCTCT TCAGGGAGCT TGAAAAGACT 
TCAGATGGTT CCGTTTTAAA GACATGGAGT CGTAATCCTG CTGTTGAGCG GAGATTGAGG 
AGAGGTCAAG ATCGATCCCG CACTCAGCCA GTCACCACAG AGGAAGTAGT TATTGCTGCT 
ACTGCCCCTG GCCATTTGTC ACAGTCCGTG ACTTTACAAA CCTCTATAAC GCGCGTTCCT 
AGTCCCACTC TAGTCAGCAC CTCTGTGACC AAACACAGCT TGCAGGCTTC GTCTCAGCAA 
AGCTCCATGG TGAGAGAGCA GGCCAGAGAG GTTCGTCTGG CTCAGGAGGC AACGGAGACA 
GATCGAGCTC GCCACGCTTC TCCGGGGGAA GGCGGACACG AGGAGCCAGA CCTTTCCACA 
CTCACTCTAG CAGAAAAGAT GGCACTTTTT AACCGTCTTG CCCAGCCGTC CAGCCACGGC 
TCTAGGGCCA GAGGAGACAC GCGTGCACGG AGGAGTAACG CACGCTATCA GACTCAACCA 
ATCACACTAG GAGAAGTCGA ACAGTTTCAG CCTGCAGGTG GAGCTCGTTT TGCACCCAGC 
TCCAACTCAG CCATGAGATC CTCCACTGTT TCCACTGAAC ATGCGGGCGA CATCCATCTG 
ACCATGCCAA ATGTATCTGG GCCTGCATAT TATGAGCAGA CCGTTTCTCC ACCGCATCAC 
CCTCAGCCTG CCCATGAGAT CCAGACACAT CAGAGTCTGG CTGAAGGGGA AAGGCTGCTG 
TTGAGCACTG GGAAGAGGAA GCTTCCCTCC CCAGACAGCG TGAAACGGAC ACCGCATGCT 
CAGCCGGCCA GAGACTGGGA CAGTAAGGCT GTGGCGGTGA CCTCTCAAGA CCTTTCACAA 
ACAGATGGAG AGCACACAAC AGATCTTCCT GTACACACTG CCGCTGCTGT ATCTGCATTA 
CAGAGCCATT CTCTCCATGA GGACCACACC AGAGAGGAAG AGCAGGAGAG CGACTTCAAA 
ACAGAAGACG GAGACGAGCT CTCGGACGTC ATGTCTGCCA GACAGATGTC CATCAAAGAG 
AGGCTGGCTC TGCTGAAGAA GAGTGGAGAG GAAGACTGGA GGAACAGGAT AAATAAAAAA 
CAGGATGTGG TGAAGGTTGC TGTGTCTGAA AGACATGCCC AGCTATGGGA GGTGGAGCAG 
AGCTTCAAGA AGAAGGATGA TGGGATGATG ATAGATGACT TTGCTGTGTC TGATCAACTC 
TGGGATCCTG TCTTTTCCTC CTCCCTTTTG TGTGCCTCTG AACCACTGGA TCAAATAGCA 
AACCTTCCCA AGGCTACCCT CCTGCCCGTG CAGGTGGATG AGCGTCACCC CTGGAGACGG 
AAGAAGATGG GAAATGAAGT TGTGGAAAGC CAACAGCTTG AGCGAGTAGA GATGTCTATT 
CAGGAACGCA AGCAGCTCAT CACTTCACAA GAGGAGGCCT GGAAAGCCAA AGGTTACGGA 
GCAGCCAACG ACTCCACTCA GTTTACAGTG GCTGGACGTA TGGTCAAAAA AGGCCTGGCA 
GCCCCTTCTG TTCTCACAAA TCCCGTGTTG TCTCCTCTGT CTTCTAAAAA CAAGAATACA 
TCCCATACCA TCACAAAACC ACATGAAGAG ATTGAAGTCA AACAGAACAT GAAGCTGGAG 
TCAGACATGA AGTTAGAAAA GTTGGAAACA TTCCTTGGTC GTTTGAATAG TAAAGTTCCA 
GGACTTCAGG AGGCCACAAT TGCTGTCACG GAGAAGTCTG TAAAAGAGGT CATGACTCTG 
GACGATGAGG TCTTTGATAA GTTTTATCGA CATGCAGAGG ATATGGCAAA GTTCACCAGC 
AGAGTGGAAA TTAGTGATGA TTTTGATGAC ATCTTTGGAG TACAGCTGCC CAGGTTGACT 
TCGGAAATGG TGCAGCACAA GCGGGCAGTT CGGCCTACCA GAAATGTTCA GTCATCTAAA 
AACCCGCTGA AAATGCTGGC TGTTCGGGAG GACATCAGAC ATGAGTACAC GGAACAAAGA 
CTGAACATCG GCCTTCTGGA GAGCAAGAGG ATGAAACAGG AAAAGATGAA TAAGAACAGC 
GGTTTCTCTG AGGTTGCTCT CGCCGGATTG GCCAGTAAGG AGAACTTCAG CAGTGTGAGT 
CTGCGAAGTG TGAATGCCTC TGAACAAAGC TCCAACAACA GTGCCATGCC CTATAAAAAA 
CTCATGCTCA TTCAGGTCAA AGGTCGCAGG CACGTTCAGA CCAGACTGGT TGAGCCCAGA 
GCTTCCTCCT TGAACAGTGG CGACTGCTTC CTCCTGATCA CACCACATCA TTGCTTCATC 
TGGATTGGAG AATTCGCTAA TGTTATTGAG AAGGCCAAAG CTGCAGAGTT GGCCACGTTC 
GTTCAAACCA AGCATGACCT GGGATGCAGA GCCTCGTATG TTCAGACAAT TGAGGAGGGT 
GCAAACACTC ACACACACGC TGCCAAAGAC TTTTGGAAGA TTCTCGGTGG CCAAACCAGT 
TATCAGTCCG CTGGCACTCC TGAACAAGAT GAGTTTTATG AGAGTGCAGT CGTAGAAACC 
AACTGCATAT ACAGACTCAT GGAGGATAAA CTTGTTCCCC ATGATGACTA CTGGGGTCGA 
GTCCCTCGCT GCTCCATGCT GAACCCTAAA GAGGTGCTGG TATTTGATTT TGGTAGTGAG 
GTCTATGTAT GGCATGGCAA GGAGGTGACG TTGGCACAGA GAAAAGTGGC ATTTCAGCTG 
GCGAAGCACT TGTGGAATGG CACCTTTGAC TACACCAACT GTGACATTAA TCCTCTGGAC 
CCTGGAGAGT GCAATGCTCT CATACCCAGG AAAGGCCAGG GACGACCAGA CTGGGCTGTG 
TTTGGCAGGT TAACTCAGCA CAATGAAACT ACTCTTTTTA AAGAGAAGTT TTTGGACTGG 
AGTGATTCCA AGAATTCTAA GAAAAATGGT GACTTGCATG TTGAAAACAA GGAGCTTCGG 
AGTGGTGAAT GTCGGCCCTA TGATGCGTCT CTGATGCTAT CTGCATTACG GGCGCCAGTC 
AGGACCGTTC TGGATGGGGT GAACATTGGA CGAGGATATG GATTAGTGGA AGGTGAAGAG 
GGACGTAATT TTGAGATCAG CACATTGTCT GTGGATGTCT GGCATATTTT AGAGTTTGAC 
TACAGTCGAC TACCCAAACA GAGCATTGGT CAGTTTCATG AAGGTGACAC ATACGTGGTC 
AAATGGAAGT ACATGATCAG CAGAGCAGTT GGCAAGAGAC TGCATTCAGA AAGAATCATC 
GGTCCAGGGA AGGAGAAATG TTGTTATTTC TTCTGGCAGG GTCGTAACTC TACGGTGAAT 
GAAAAGGGAA CGTCAGCACT GATGACTGTT GAGCTGGATG AGGAAAGAGG AGCTCAGGTC 
CAGGTACAGC AAGGAAAGGA GCCTCCTTGT TTCCTACAGT GCTTCAATGG AGGAATGATT 
GTTCATGCTG GAAAAAGAGA AGAGGAGGAA GACAATACAC AAAACGACTG GCGATTGTAC 
TGTGTGAGAG GAGAGAAACC CATAGAGGGC CATCTGCTGG AGGCAGTGTG TCACTGCAGC 
AGCTTGCGAT CCCGGACCTC TATGATCCTC CTGAACATCC CCAAAGCCAG CCTATACCTG 
TGGCATGGCT GCAAGGCACA AGTGCACACA CGTGACGTGG GCCGTACCAC TGCAAACAAA 
ATCAAAGAGC AATGTCCTCT GGAGGCAGGG CTTCACAGCA GCAGTAAAGT GTCCATCCAG 
GAGTGTGATG AGGGAGCTGA ACCACAGGGC TTCTGGGAGG CTTTGGGCAA AAGAGATCGA 
AAAGCGTACG ACTGCATGCT GCAGGATCCA GGAAAGTTCA ACTTCACTCC TCGGCTTTTC 
CAGTTAAGCA GTGCCTCGGG AGAGTTTGCA GCAGTGGAGT TTGTATATCC ATCCAGAGAA 
CCAAATTTGG TTAACTCGAT GCCTTTCCTC CAGGAGGATT TGTATACAGC TACTCAGCCA 
GCCCTGTTTT TGGTGGATAA TCATCATGAA GTTTACCTGT GGCAGGGCTG GTGGCCTCAG 
GACAGTGAAA GCACAGGCTC AGCTCGCATA CGGTGGGACT CTGACAGGAA GTGTGCAATG 
GAAACTGTGC TCCAGTATAG CAAAGAAAAA AATGAGAAAA AGACACCAAA ATCCTACCTG 
ATCCATGCAG GACTGGAGCC TCTTACATTC ACCAACATGT TTCCCAGCTG GGAACACAGA 
GAGGACATCG CTGAAATCAC AGAGAAGGAG GCCGAAGTAT GTCATCAGAT CATTCTTGTT 
GAAGATGTCT TGAGCCGACT CTGCAAGACC ACTTACCCAC TGGCTGAACT AAAAGCACGT 
CCCTTACCGG AAGGTGTCGA CCCTCTACGT CTTGAAACCT ACTTATCAGA TGAGGACTTT 
GAGGGTGTAG GGGCTTCTGG GAAGAAAGCT TTGGAGATGA CGAGAAGTGA ATATGAGGCA 
CTGCCTGGAT GGAAGCAAGT GAATGTGAAG AAAGCCAAAG GATTGTTTTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017213912.1
CDS	340..6990
Translation	MYRNPWISNYINHVKLCQGFTRETAPKVGNTKQRRALSFQKEFSFEEKGEGANKEVDVSLLGNTPKVSELRKRFESISTSQSWDMNRKERIARRLEGIDNDVLQSLPGCGGLVSNRLLEEDTPRYTRAADTCDPCTVSLQHYGKSEPSSQSTDVPSRSYAVHPESVFSTGSSHVAELDSKAERIARYKAERRRQLAERYGISLDQEMETDYTTRFSRPSRELDGSDRHHRSKSEGRDGVEQNAYTSSHHVDKSSSDYHSSPEYSRERTDSISERERMMNLENQRRAQERDRLHGGGGVAPDPSAYMDVSGSARVPGREPGMMGVPSSPNSGRRGLMLSPKQGASPGDLFIEQQAHSILQRHGIRVRERLARDEPFHRGQTTEEPAYNQSYLRYYPHSQSLLDGPVKQQQLTRSEKKERHDMGCQSYFSMGSESTSRNKQEIQEVVEEMEGKHVARTEGLLRSRKAVLPSEIRRKERNTDDSCSGSGDNREVSSAIAGHRSSEGAANDPRGWHGSRARREVYTEHVSRLQAAESLQARAREAHRRPDASCCAPIQTSSARPMTNSSKPMDTGNTQKTVLPIQEPIREAVQQEKVPNVDHLSHDARVSVAHLRHTYLQSVASPRKAELAEELPAVTDVPWRSMRVRDRSSRRPLRYICPTESRKTSERFRTQPITSTEREQSDRSHLSSEPSSPEVDEEKLDERAKLSVAAKRSLFRELEKTSDGSVLKTWSRNPAVERRLRRGQDRSRTQPVTTEEVVIAATAPGHLSQSVTLQTSITRVPSPTLVSTSVTKHSLQASSQQSSMVREQAREVRLAQEATETDRARHASPGEGGHEEPDLSTLTLAEKMALFNRLAQPSSHGSRARGDTRARRSNARYQTQPITLGEVEQFQPAGGARFAPSSNSAMRSSTVSTEHAGDIHLTMPNVSGPAYYEQTVSPPHHPQPAHEIQTHQSLAEGERLLLSTGKRKLPSPDSVKRTPHAQPARDWDSKAVAVTSQDLSQTDGEHTTDLPVHTAAAVSALQSHSLHEDHTREEEQESDFKTEDGDELSDVMSARQMSIKERLALLKKSGEEDWRNRINKKQDVVKVAVSERHAQLWEVEQSFKKKDDGMMIDDFAVSDQLWDPVFSSSLLCASEPLDQIANLPKATLLPVQVDERHPWRRKKMGNEVVESQQLERVEMSIQERKQLITSQEEAWKAKGYGAANDSTQFTVAGRMVKKGLAAPSVLTNPVLSPLSSKNKNTSHTITKPHEEIEVKQNMKLESDMKLEKLETFLGRLNSKVPGLQEATIAVTEKSVKEVMTLDDEVFDKFYRHAEDMAKFTSRVEISDDFDDIFGVQLPRLTSEMVQHKRAVRPTRNVQSSKNPLKMLAVREDIRHEYTEQRLNIGLLESKRMKQEKMNKNSGFSEVALAGLASKENFSSVSLRSVNASEQSSNNSAMPYKKLMLIQVKGRRHVQTRLVEPRASSLNSGDCFLLITPHHCFIWIGEFANVIEKAKAAELATFVQTKHDLGCRASYVQTIEEGANTHTHAAKDFWKILGGQTSYQSAGTPEQDEFYESAVVETNCIYRLMEDKLVPHDDYWGRVPRCSMLNPKEVLVFDFGSEVYVWHGKEVTLAQRKVAFQLAKHLWNGTFDYTNCDINPLDPGECNALIPRKGQGRPDWAVFGRLTQHNETTLFKEKFLDWSDSKNSKKNGDLHVENKELRSGECRPYDASLMLSALRAPVRTVLDGVNIGRGYGLVEGEEGRNFEISTLSVDVWHILEFDYSRLPKQSIGQFHEGDTYVVKWKYMISRAVGKRLHSERIIGPGKEKCCYFFWQGRNSTVNEKGTSALMTVELDEERGAQVQVQQGKEPPCFLQCFNGGMIVHAGKREEEEDNTQNDWRLYCVRGEKPIEGHLLEAVCHCSSLRSRTSMILLNIPKASLYLWHGCKAQVHTRDVGRTTANKIKEQCPLEAGLHSSSKVSIQECDEGAEPQGFWEALGKRDRKAYDCMLQDPGKFNFTPRLFQLSSASGEFAAVEFVYPSREPNLVNSMPFLQEDLYTATQPALFLVDNHHEVYLWQGWWPQDSESTGSARIRWDSDRKCAMETVLQYSKEKNEKKTPKSYLIHAGLEPLTFTNMFPSWEHREDIAEITEKEAEVCHQIILVEDVLSRLCKTTYPLAELKARPLPEGVDPLRLETYLSDEDFEGVGASGKKALEMTRSEYEALPGWKQVNVKKAKGLF

Target ORF information:

RefSeq Version	XM_017358423.1
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio supervillin a (svila), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017358423.1

ORF Insert Sequence:

ATGTATCGGA ACCCTTGGAT CTCCAACTAT ATCAACCATG TCAAGTTGTG CCAAGGCTTC 
ACCAGAGAAA CAGCCCCTAA AGTTGGCAAT ACCAAGCAAC GGAGAGCTCT TAGTTTTCAG 
AAAGAGTTCT CGTTTGAGGA GAAAGGTGAG GGAGCCAACA AAGAAGTAGA TGTTAGTCTG 
CTGGGGAATA CACCCAAGGT CTCAGAATTA AGGAAACGTT TTGAAAGTAT AAGCACAAGT 
CAGAGTTGGG ACATGAACAG GAAGGAGCGA ATTGCACGGC GCCTGGAAGG AATCGATAAT 
GATGTTCTCC AAAGTCTTCC AGGTTGTGGT GGTCTAGTTT CTAATCGACT GCTGGAGGAG 
GACACTCCAA GATATACCCG TGCAGCAGAC ACCTGTGACC CCTGTACAGT TTCTCTTCAG 
CACTATGGCA AAAGTGAACC AAGTTCACAA AGCACAGATG TTCCATCCAG AAGTTACGCA 
GTCCACCCGG AGTCTGTCTT CAGCACTGGC TCCTCTCATG TGGCAGAGCT GGACTCTAAA 
GCAGAAAGGA TTGCACGCTA CAAGGCTGAA CGCCGCCGTC AGTTAGCAGA GCGCTATGGC 
ATCTCTCTAG ATCAGGAGAT GGAAACAGAC TATACAACTC GCTTCTCCAG ACCCTCCAGA 
GAGCTTGATG GTTCAGACAG ACACCACAGG TCAAAATCAG AAGGGAGAGA TGGAGTCGAG 
CAAAATGCTT ACACCAGCAG CCATCATGTG GATAAGAGCA GCTCAGACTA TCACTCCAGT 
CCAGAGTACA GCCGCGAGCG CACAGACTCC ATCTCAGAAC GTGAAAGAAT GATGAATCTG 
GAGAACCAGA GGAGGGCACA GGAGCGTGAC CGATTACATG GAGGTGGAGG GGTTGCACCT 
GACCCATCTG CGTATATGGA TGTGTCTGGA TCTGCAAGGG TACCAGGAAG GGAGCCAGGG 
ATGATGGGAG TTCCCAGCTC ACCCAATTCA GGCCGAAGGG GCTTGATGCT CTCTCCCAAA 
CAGGGAGCGT CTCCTGGTGA TCTGTTTATT GAGCAGCAGG CTCACAGCAT CCTCCAAAGA 
CACGGAATCA GAGTTAGAGA GCGACTGGCC CGCGATGAGC CATTTCACAG GGGTCAAACA 
ACAGAGGAAC CTGCTTACAA CCAGTCATAT CTTCGTTATT ACCCTCACTC ACAGAGCCTC 
CTAGATGGTC CTGTCAAACA GCAGCAACTG ACCCGTTCTG AAAAAAAGGA ACGCCATGAT 
ATGGGTTGCC AGAGTTACTT CTCCATGGGC AGCGAATCCA CTTCCAGGAA TAAGCAAGAG 
ATTCAGGAGG TGGTTGAAGA AATGGAGGGA AAACATGTTG CGAGGACTGA AGGTCTTCTG 
CGGAGCAGAA AGGCAGTTTT ACCTTCTGAG ATTCGAAGGA AGGAACGGAA CACAGACGAT 
TCATGCTCTG GAAGTGGTGA CAACAGGGAG GTAAGCTCAG CTATTGCTGG GCACAGGAGC 
AGCGAGGGTG CTGCAAATGA CCCCAGAGGG TGGCATGGAA GTCGGGCAAG ACGGGAAGTT 
TATACAGAGC ACGTGTCTCG ACTTCAGGCT GCAGAGTCTT TGCAGGCAAG AGCTCGAGAA 
GCTCATAGAA GGCCTGATGC TAGCTGCTGC GCCCCAATCC AGACCTCCAG TGCAAGACCA 
ATGACAAATT CATCTAAACC CATGGATACT GGAAATACAC AAAAAACTGT TCTCCCCATC 
CAGGAGCCAA TCAGAGAGGC TGTCCAACAA GAAAAAGTGC CAAATGTTGA TCACCTGTCC 
CATGATGCCA GGGTTTCAGT AGCCCATCTC CGCCACACAT ACCTGCAAAG TGTTGCAAGT 
CCCCGGAAAG CCGAACTGGC AGAAGAGCTG CCTGCAGTTA CAGATGTACC CTGGAGAAGC 
ATGCGAGTCA GAGATCGGTC ATCTCGTCGC CCTTTGCGAT ATATATGTCC CACTGAAAGT 
AGAAAGACAT CTGAGAGGTT TAGAACACAG CCAATCACAT CAACCGAGCG TGAGCAGTCG 
GACCGGTCTC ATCTAAGCTC TGAACCATCA AGCCCTGAGG TGGATGAAGA GAAACTAGAT 
GAGAGAGCAA AGCTGAGCGT AGCTGCCAAG CGTTCCCTCT TCAGGGAGCT TGAAAAGACT 
TCAGATGGTT CCGTTTTAAA GACATGGAGT CGTAATCCTG CTGTTGAGCG GAGATTGAGG 
AGAGGTCAAG ATCGATCCCG CACTCAGCCA GTCACCACAG AGGAAGTAGT TATTGCTGCT 
ACTGCCCCTG GCCATTTGTC ACAGTCCGTG ACTTTACAAA CCTCTATAAC GCGCGTTCCT 
AGTCCCACTC TAGTCAGCAC CTCTGTGACC AAACACAGCT TGCAGGCTTC GTCTCAGCAA 
AGCTCCATGG TGAGAGAGCA GGCCAGAGAG GTTCGTCTGG CTCAGGAGGC AACGGAGACA 
GATCGAGCTC GCCACGCTTC TCCGGGGGAA GGCGGACACG AGGAGCCAGA CCTTTCCACA 
CTCACTCTAG CAGAAAAGAT GGCACTTTTT AACCGTCTTG CCCAGCCGTC CAGCCACGGC 
TCTAGGGCCA GAGGAGACAC GCGTGCACGG AGGAGTAACG CACGCTATCA GACTCAACCA 
ATCACACTAG GAGAAGTCGA ACAGTTTCAG CCTGCAGGTG GAGCTCGTTT TGCACCCAGC 
TCCAACTCAG CCATGAGATC CTCCACTGTT TCCACTGAAC ATGCGGGCGA CATCCATCTG 
ACCATGCCAA ATGTATCTGG GCCTGCATAT TATGAGCAGA CCGTTTCTCC ACCGCATCAC 
CCTCAGCCTG CCCATGAGAT CCAGACACAT CAGAGTCTGG CTGAAGGGGA AAGGCTGCTG 
TTGAGCACTG GGAAGAGGAA GCTTCCCTCC CCAGACAGCG TGAAACGGAC ACCGCATGCT 
CAGCCGGCCA GAGACTGGGA CAGTAAGGCT GTGGCGGTGA CCTCTCAAGA CCTTTCACAA 
ACAGATGGAG AGCACACAAC AGATCTTCCT GTACACACTG CCGCTGCTGT ATCTGCATTA 
CAGAGCCATT CTCTCCATGA GGACCACACC AGAGAGGAAG AGCAGGAGAG CGACTTCAAA 
ACAGAAGACG GAGACGAGCT CTCGGACGTC ATGTCTGCCA GACAGATGTC CATCAAAGAG 
AGGCTGGCTC TGCTGAAGAA GAGTGGAGAG GAAGACTGGA GGAACAGGAT AAATAAAAAA 
CAGGATGTGG TGAAGGTTGC TGTGTCTGAA AGACATGCCC AGCTATGGGA GGTGGAGCAG 
AGCTTCAAGA AGAAGGATGA TGGGATGATG ATAGATGACT TTGCTGTGTC TGATCAACTC 
TGGGATCCTG TCTTTTCCTC CTCCCTTTTG TGTGCCTCTG AACCACTGGA TCAAATAGCA 
AACCTTCCCA AGGCTACCCT CCTGCCCGTG CAGGTGGATG AGCGTCACCC CTGGAGACGG 
AAGAAGATGG GAAATGAAGT TGTGGAAAGC CAACAGCTTG AGCGAGTAGA GATGTCTATT 
CAGGAACGCA AGCAGCTCAT CACTTCACAA GAGGAGGCCT GGAAAGCCAA AGGTTACGGA 
GCAGCCAACG ACTCCACTCA GTTTACAGTG GCTGGACGTA TGGTCAAAAA AGGCCTGGCA 
GCCCCTTCTG TTCTCACAAA TCCCGTGTTG TCTCCTCTGT CTTCTAAAAA CAAGAATACA 
TCCCATACCA TCACAAAACC ACATGAAGAG ATTGAAGTCA AACAGAACAT GAAGCTGGAG 
TCAGACATGA AGTTAGAAAA GTTGGAAACA TTCCTTGGTC GTTTGAATAG TAAAGTTCCA 
GGACTTCAGG AGGCCACAAT TGCTGTCACG GAGAAGTCTG TAAAAGAGGT CATGACTCTG 
GACGATGAGG TCTTTGATAA GTTTTATCGA CATGCAGAGG ATATGGCAAA GTTCACCAGC 
AGAGTGGAAA TTAGTGATGA TTTTGATGAC ATCTTTGGAG TACAGCTGCC CAGGTTGACT 
TCGGAAATGG TGCAGCACAA GCGGGCAGTT CGGCCTACCA GAAATGTTCA GTCATCTAAA 
AACCCGCTGA AAATGCTGGC TGTTCGGGAG GACATCAGAC ATGAGTACAC GGAACAAAGA 
CTGAACATCG GCCTTCTGGA GAGCAAGAGG ATGAAACAGG AAAAGATGAA TAAGAACAGC 
GGTTTCTCTG AGGTTGCTCT CGCCGGATTG GCCAGTAAGG AGAACTTCAG CAGTGTGAGT 
CTGCGAAGTG TGAATGCCTC TGAACAAAGC TCCAACAACA GTGCCATGCC CTATAAAAAA 
CTCATGCTCA TTCAGGTCAA AGGTCGCAGG CACGTTCAGA CCAGACTGGT TGAGCCCAGA 
GCTTCCTCCT TGAACAGTGG CGACTGCTTC CTCCTGATCA CACCACATCA TTGCTTCATC 
TGGATTGGAG AATTCGCTAA TGTTATTGAG AAGGCCAAAG CTGCAGAGTT GGCCACGTTC 
GTTCAAACCA AGCATGACCT GGGATGCAGA GCCTCGTATG TTCAGACAAT TGAGGAGGGT 
GCAAACACTC ACACACACGC TGCCAAAGAC TTTTGGAAGA TTCTCGGTGG CCAAACCAGT 
TATCAGTCCG CTGGCACTCC TGAACAAGAT GAGTTTTATG AGAGTGCAGT CGTAGAAACC 
AACTGCATAT ACAGACTCAT GGAGGATAAA CTTGTTCCCC ATGATGACTA CTGGGGTCGA 
GTCCCTCGCT GCTCCATGCT GAACCCTAAA GAGGTGCTGG TATTTGATTT TGGTAGTGAG 
GTCTATGTAT GGCATGGCAA GGAGGTGACG TTGGCACAGA GAAAAGTGGC ATTTCAGCTG 
GCGAAGCACT TGTGGAATGG CACCTTTGAC TACACCAACT GTGACATTAA TCCTCTGGAC 
CCTGGAGAGT GCAATGCTCT CATACCCAGG AAAGGCCAGG GACGACCAGA CTGGGCTGTG 
TTTGGCAGGT TAACTCAGCA CAATGAAACT ACTCTTTTTA AAGAGAAGTT TTTGGACTGG 
AGTGATTCCA AGAATTCTAA GAAAAATGGT GACTTGCATG TTGAAAACAA GGAGCTTCGG 
AGTGGTGAAT GTCGGCCCTA TGATGCGTCT CTGATGCTAT CTGCATTACG GGCGCCAGTC 
AGGACCGTTC TGGATGGGGT GAACATTGGA CGAGGATATG GATTAGTGGA AGGTGAAGAG 
GGACGTAATT TTGAGATCAG CACATTGTCT GTGGATGTCT GGCATATTTT AGAGTTTGAC 
TACAGTCGAC TACCCAAACA GAGCATTGGT CAGTTTCATG AAGGTGACAC ATACGTGGTC 
AAATGGAAGT ACATGATCAG CAGAGCAGTT GGCAAGAGAC TGCATTCAGA AAGAATCATC 
GGTCCAGGGA AGGAGAAATG TTGTTATTTC TTCTGGCAGG GTCGTAACTC TACGGTGAAT 
GAAAAGGGAA CGTCAGCACT GATGACTGTT GAGCTGGATG AGGAAAGAGG AGCTCAGGTC 
CAGGTACAGC AAGGAAAGGA GCCTCCTTGT TTCCTACAGT GCTTCAATGG AGGAATGATT 
GTTCATGCTG GAAAAAGAGA AGAGGAGGAA GACAATACAC AAAACGACTG GCGATTGTAC 
TGTGTGAGAG GAGAGAAACC CATAGAGGGC CATCTGCTGG AGGCAGTGTG TCACTGCAGC 
AGCTTGCGAT CCCGGACCTC TATGATCCTC CTGAACATCC CCAAAGCCAG CCTATACCTG 
TGGCATGGCT GCAAGGCACA AGTGCACACA CGTGACGTGG GCCGTACCAC TGCAAACAAA 
ATCAAAGAGC AATGTCCTCT GGAGGCAGGG CTTCACAGCA GCAGTAAAGT GTCCATCCAG 
GAGTGTGATG AGGGAGCTGA ACCACAGGGC TTCTGGGAGG CTTTGGGCAA AAGAGATCGA 
AAAGCGTACG ACTGCATGCT GCAGGATCCA GGAAAGTTCA ACTTCACTCC TCGGCTTTTC 
CAGTTAAGCA GTGCCTCGGG AGAGTTTGCA GCAGTGGAGT TTGTATATCC ATCCAGAGAA 
CCAAATTTGG TTAACTCGAT GCCTTTCCTC CAGGAGGATT TGTATACAGC TACTCAGCCA 
GCCCTGTTTT TGGTGGATAA TCATCATGAA GTTTACCTGT GGCAGGGCTG GTGGCCTCAG 
GACAGTGAAA GCACAGGCTC AGCTCGCATA CGGTGGGACT CTGACAGGAA GTGTGCAATG 
GAAACTGTGC TCCAGTATAG CAAAGAAAAA AATGAGAAAA AGACACCAAA ATCCTACCTG 
ATCCATGCAG GACTGGAGCC TCTTACATTC ACCAACATGT TTCCCAGCTG GGAACACAGA 
GAGGACATCG CTGAAATCAC AGAGAAGGAG GCCGAAGTAT GTCATCAGAT CATTCTTGTT 
GAAGATGTCT TGAGCCGACT CTGCAAGACC ACTTACCCAC TGGCTGAACT AAAAGCACGT 
CCCTTACCGG AAGGTGTCGA CCCTCTACGT CTTGAAACCT ACTTATCAGA TGAGGACTTT 
GAGGGTGTAG GGGCTTCTGG GAAGAAAGCT TTGGAGATGA CGAGAAGTGA ATATGAGGCA 
CTGCCTGGAT GGAAGCAAGT GAATGTGAAG AAAGCCAAAG GATTGTTTTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Evolution of complexity in the zebrafish synapse proteome.
Nature communications814613(2017 Mar)
Bay?s ?,Collins MO,Reig-Viader R,Gou G,Goulding D,Izquierdo A,Choudhary JS,Emes RD,Grant SG

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
BMC genomics17368(2016 05)
Pasquier J,Cabau C,Nguyen T,Jouanno E,Severac D,Braasch I,Journot L,Pontarotti P,Klopp C,Postlethwait JH,Guiguen Y,Bobe J

Supervillin Is a Component of the Hair Cell's Cuticular Plate and the Head Plates of Organ of Corti Supporting Cells.
PloS one11(7)e0158349(2016)
Pollock LM,Gupta N,Chen X,Luna EJ,McDermott BM Jr

RFX transcription factors are essential for hearing in mice.
Nature communications68549(2015 Oct)
Elkon R,Milon B,Morrison L,Shah M,Vijayakumar S,Racherla M,Leitch CC,Silipino L,Hadi S,Weiss-Gayet M,Barras E,Schmid CD,Ait-Lounis A,Barnes A,Song Y,Eisenman DJ,Eliyahu E,Frolenkov GI,Strome SE,Durand B,Zaghloul NA,Jones SM,Reith W,Hertzano R

Prioritizing causal disease genes using unbiased genomic features.
Genome biology15(12)534(2014 Dec)
Deo RC,Musso G,Tasan M,Tang P,Poon A,Yuan C,Felix JF,Vasan RS,Beroukhim R,De Marco T,Kwok PY,MacRae CA,Roth FP

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svila cDNA ORF clone, Danio rerio(zebrafish)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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