rack1 cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Gene Symbol	rack1
Entrez Gene ID	30722
Full Name	receptor for activated C kinase 1
Synonyms	gnb2l1,wu:fb80d08,wu:fk65d12
General protein information	Preferred Names receptor for activated C kinase 1 Names guanine nucleotide-binding protein subunit beta-2-like 1 RACK guanine nucleotide binding protein (G protein), beta polypeptide 2-like 1 receptor for activated protein kinase C receptor of activated protein kinase C
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA Protein Name

NM_131444.1 NP_571519.1 guanine nucleotide-binding protein subunit beta-2-like 1
Pathways from BioSystems

mRNA	Protein	Name
NM_131444.1	NP_571519.1	guanine nucleotide-binding protein subunit beta-2-like 1

Homologies

Homo sapiens (human)	GNB2L1	NP_006089.1
Pan troglodytes (chimpanzee)	GNB2L1	XP_518165.3
	LOC708526	XP_001105066.1
Caenorhabditis elegans (roundworm)	rack-1	NP_501859.1
Kluyveromyces lactis	KLLA0E12277g	XP_454502.1
Schizosaccharomyces pombe (fission yeast)	cpc2	NP_593770.1
Arabidopsis thaliana (thale cress)	ATARCA	NP_173248.1
Bos taurus (cattle)	GNB2L1	XP_005209294.1
Mus musculus (house mouse)	Gnb2l1	NP_032169.1
Rattus norvegicus (Norway rat)	Gnb2l1	NP_570090.2
Magnaporthe oryzae (rice blast fungus)	MGG_04719	XP_003710816.1
Arabidopsis thaliana (thale cress)	RACK1B_AT	NP_175296.1
Gallus gallus (chicken)	GNB2L1	NP_001004378.1
Drosophila melanogaster (fruit fly)	Rack1	NP_001260218.1
Saccharomyces cerevisiae (baker's yeast)	ASC1	NP_013834.1
Eremothecium gossypii	AGOS_AFR199C	NP_985746.1
Arabidopsis thaliana (thale cress)	RACK1C_AT	NP_188441.1
Oryza sativa (rice)	Os01g0686800	NP_001043910.1
Oryza sativa (rice)	Os05g0552300	NP_001056254.1
Canis lupus familiaris (dog)	GNB2L1	XP_005626337.1
Danio rerio (zebrafish)	gnb2l1	NP_571519.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP010173	XP_319347.2
Neurospora crassa	NCU05810	XP_960070.1
Xenopus tropicalis (tropical clawed frog)	gnb2l1	NP_001004946.1

Gene Ontology
Bibliography

Related articles in PubMed

Evolution of complexity in the zebrafish synapse proteome.
Bay?s ?, Collins MO, Reig-Viader R, Gou G, Goulding D, Izquierdo A, Choudhary JS, Emes RD, Grant SG
Nature communications814613(2017 Mar)

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
Pasquier J, Cabau C, Nguyen T, Jouanno E, Severac D, Braasch I, Journot L, Pontarotti P, Klopp C, Postlethwait JH, Guiguen Y, Bobe J
BMC genomics17368(2016 05)

RFX transcription factors are essential for hearing in mice.
Elkon R, Milon B, Morrison L, Shah M, Vijayakumar S, Racherla M, Leitch CC, Silipino L, Hadi S, Weiss-Gayet M, Barras E, Schmid CD, Ait-Lounis A, Barnes A, Song Y, Eisenman DJ, Eliyahu E, Frolenkov GI, Strome SE, Durand B, Zaghloul NA, Jones SM, Reith W, Hertzano R
Nature communications68549(2015 Oct)

Increased functional protein expression using nucleotide sequence features enriched in highly expressed genes in zebrafish.
Horstick EJ, Jordan DC, Bergeron SA, Tabor KM, Serpe M, Feldman B, Burgess HA
Nucleic acids research43(7)e48(2015 Apr)

Histopathologic alterations associated with global gene expression due to chronic dietary TCDD exposure in juvenile zebrafish.
Liu Q, Spitsbergen JM, Cariou R, Huang CY, Jiang N, Goetz G, Hutz RJ, Tonellato PJ, Carvan MJ 3rd
PloS one9(7)e100910(2014)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Rack1 has a dominant-negative effect on Vangl2 localization and gastrulation.
Title: Rack1 is required for Vangl2 membrane localization and planar cell polarity signaling while attenuating canonical Wnt activity.

The following rack1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the rack1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ODa37605	NM_131444.1 Latest version!	Danio rerio receptor for activated C kinase 1 (rack1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$174.30 $249.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

rack1 ( NM_131444.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_571519.1 Danio rerio receptor for activated C kinase 1 (rack1), mRNA.

Price & Availability↑

CloneID	ODa37605
Clone ID Related Accession (Same CDS sequence)	NM_131444.1
Accession Version	NM_131444.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 954bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-24
Organism	Danio rerio(zebrafish)
Product	guanine nucleotide-binding protein subunit beta-2-like 1
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AF025330.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF025330.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2168446, SAMEA2168447 [ECO:0000348] ##Evidence-Data-END##

ATGACCGAGC AGATGACAGT AAGGGGTACC CTGAAGGGTC ACAGCGGATG GGTCACCCAA 
ATCGCCACCA CACCCCAGTT TCCCGACATG ATTCTGTCCG CGTCCCGGGA CAAGACCATC 
ATCATGTGGA AGCTGACCCG TGACGAGACC AACTATGGCA TCCCCCAGCG TGCTCTGAGG 
GGCCATTCCC ACTTTGTGAG TGATGTTGTA ATCTCATCTG ATGGCCAGTT TGCTCTGTCT 
GGATCCTGGG ACGGGACCCT GCGCCTGTGG GATCTCACAA CTGGCACTAC CACCCGCCGC 
TTTGTTGGGC ACACCAAAGA TGTTCTGAGC GTGGCTTTCT CTGCTGACAA CCGTCAGATC 
GTGTCTGGAT CCAGAGACAA GACCATCAAG CTGTGGAACA CTCTGGGAGT CTGCAAGTAC 
ACCATCCAGG ATGACAGCCA CACTGAGTGG GTGTCCTGTG TGCGCTTCTC TCCCAACAGC 
AGCAACCCCA TCATTGTGTC CTGCGGGTGG GATAAAATGG TCAAGGTATG GAATTTGGCT 
AACTGCAAGC TGAAGACCAA CCACATTGGC CACACCGGGT ACCTGAACAC AGTTACCGTG 
TCTCCTGATG GATCTCTGTG TGCCTCCGGT GGAAAGGATG GACAGGCCAT GCTGTGGGAC 
CTGAATGAGG GCAAGCACCT TTACACGCTG GACGGTGGTG ACACCATCAA CGCCCTGTGC 
TTCAGCCCCA ACCGCTACTG GCTGTGCGCT GCCACTGGAC CCAGCATCAA GATCTGGGAT 
CTGGAGGGCA AGATCATTGT TGATGAGCTG AGGCAGGACA TCATTACCAC CAACAGCAAA 
GCTGAGCCGC CTCAGTGCAC ATCTCTGGCC TGGTCTGCTG ATGGACAGAC TCTGTTTGCT 
GGCTACACTG ACAACCTGAT CAGAGTGTGG CAGGTGACCA TCGGAACCAG ATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_571519.1
CDS	58..1011
Translation	MTEQMTVRGTLKGHSGWVTQIATTPQFPDMILSASRDKTIIMWKLTRDETNYGIPQRALRGHSHFVSDVVISSDGQFALSGSWDGTLRLWDLTTGTTTRRFVGHTKDVLSVAFSADNRQIVSGSRDKTIKLWNTLGVCKYTIQDDSHTEWVSCVRFSPNSSNPIIVSCGWDKMVKVWNLANCKLKTNHIGHTGYLNTVTVSPDGSLCASGGKDGQAMLWDLNEGKHLYTLDGGDTINALCFSPNRYWLCAATGPSIKIWDLEGKIIVDELRQDIITTNSKAEPPQCTSLAWSADGQTLFAGYTDNLIRVWQVTIGTR

Target ORF information:

RefSeq Version	NM_131444.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio receptor for activated C kinase 1 (rack1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_131444.1

ORF Insert Sequence:

ATGACCGAGC AGATGACAGT AAGGGGTACC CTGAAGGGTC ACAGCGGATG GGTCACCCAA 
ATCGCCACCA CACCCCAGTT TCCCGACATG ATTCTGTCCG CGTCCCGGGA CAAGACCATC 
ATCATGTGGA AGCTGACCCG TGACGAGACC AACTATGGCA TCCCCCAGCG TGCTCTGAGG 
GGCCATTCCC ACTTTGTGAG TGATGTTGTA ATCTCATCTG ATGGCCAGTT TGCTCTGTCT 
GGATCCTGGG ACGGGACCCT GCGCCTGTGG GATCTCACAA CTGGCACTAC CACCCGCCGC 
TTTGTTGGGC ACACCAAAGA TGTTCTGAGC GTGGCTTTCT CTGCTGACAA CCGTCAGATC 
GTGTCTGGAT CCAGAGACAA GACCATCAAG CTGTGGAACA CTCTGGGAGT CTGCAAGTAC 
ACCATCCAGG ATGACAGCCA CACTGAGTGG GTGTCCTGTG TGCGCTTCTC TCCCAACAGC 
AGCAACCCCA TCATTGTGTC CTGCGGGTGG GATAAAATGG TCAAGGTATG GAATTTGGCT 
AACTGCAAGC TGAAGACCAA CCACATTGGC CACACCGGGT ACCTGAACAC AGTTACCGTG 
TCTCCTGATG GATCTCTGTG TGCCTCCGGT GGAAAGGATG GACAGGCCAT GCTGTGGGAC 
CTGAATGAGG GCAAGCACCT TTACACGCTG GACGGTGGTG ACACCATCAA CGCCCTGTGC 
TTCAGCCCCA ACCGCTACTG GCTGTGCGCT GCCACTGGAC CCAGCATCAA GATCTGGGAT 
CTGGAGGGCA AGATCATTGT TGATGAGCTG AGGCAGGACA TCATTACCAC CAACAGCAAA 
GCTGAGCCGC CTCAGTGCAC ATCTCTGGCC TGGTCTGCTG ATGGACAGAC TCTGTTTGCT 
GGCTACACTG ACAACCTGAT CAGAGTGTGG CAGGTGACCA TCGGAACCAG ATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Evolution of complexity in the zebrafish synapse proteome.
Nature communications814613(2017 Mar)
Bay?s ?,Collins MO,Reig-Viader R,Gou G,Goulding D,Izquierdo A,Choudhary JS,Emes RD,Grant SG

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
BMC genomics17368(2016 05)
Pasquier J,Cabau C,Nguyen T,Jouanno E,Severac D,Braasch I,Journot L,Pontarotti P,Klopp C,Postlethwait JH,Guiguen Y,Bobe J

RFX transcription factors are essential for hearing in mice.
Nature communications68549(2015 Oct)
Elkon R,Milon B,Morrison L,Shah M,Vijayakumar S,Racherla M,Leitch CC,Silipino L,Hadi S,Weiss-Gayet M,Barras E,Schmid CD,Ait-Lounis A,Barnes A,Song Y,Eisenman DJ,Eliyahu E,Frolenkov GI,Strome SE,Durand B,Zaghloul NA,Jones SM,Reith W,Hertzano R

Increased functional protein expression using nucleotide sequence features enriched in highly expressed genes in zebrafish.
Nucleic acids research43(7)e48(2015 Apr)
Horstick EJ,Jordan DC,Bergeron SA,Tabor KM,Serpe M,Feldman B,Burgess HA

Histopathologic alterations associated with global gene expression due to chronic dietary TCDD exposure in juvenile zebrafish.
PloS one9(7)e100910(2014)
Liu Q,Spitsbergen JM,Cariou R,Huang CY,Jiang N,Goetz G,Hutz RJ,Tonellato PJ,Carvan MJ 3rd

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rack1 cDNA ORF clone, Danio rerio(zebrafish)

Gene

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gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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