phb2a cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Gene Symbol	phb2a
Entrez Gene ID	324421
Full Name	prohibitin 2a
Synonyms	bcap37,phb2,wu:fc28c08,zgc:73150
General protein information	Preferred Names prohibitin 2a Names prohibitin 2a B-cell receptor-associated protein 37 prohibitin 2
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA Protein Name

XM_017357679.2 XP_017213168.1 prohibitin 2a isoform X1

XM_017357679.1 XP_017213168.1 prohibitin 2a isoform X1

NM_199681.2 NP_955975.1 prohibitin 2a
Pathways from BioSystems

mRNA	Protein	Name
XM_017357679.2	XP_017213168.1	prohibitin 2a isoform X1
XM_017357679.1	XP_017213168.1	prohibitin 2a isoform X1
NM_199681.2	NP_955975.1	prohibitin 2a

Homologies

Danio rerio (zebrafish)	phb2b	NP_001002681.2
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP009642	XP_318676.3
Saccharomyces cerevisiae (baker's yeast)	PHB2	NP_011747.2
Kluyveromyces lactis	KLLA0E15731g	XP_454659.1
Arabidopsis thaliana (thale cress)	PHB2	NP_973756.1
Eremothecium gossypii	AGOS_ADL141W	NP_983955.1
Magnaporthe oryzae (rice blast fungus)	MGG_09886	XP_003709979.1
Arabidopsis thaliana (thale cress)	PHB1	NP_194580.1
Homo sapiens (human)	PHB2	NP_001138303.1
	PHB2	XP_001111957.1
Bos taurus (cattle)	PHB2	NP_001039663.1
Mus musculus (house mouse)	Phb2	NP_031557.2
Rattus norvegicus (Norway rat)	Phb2	NP_001013053.1
Drosophila melanogaster (fruit fly)	l(2)03709	NP_001097372.1
Xenopus tropicalis (tropical clawed frog)	phb2	NP_001016551.1
Canis lupus familiaris (dog)	PHB2	XP_543843.3
Gallus gallus (chicken)	PHB2	NP_001074354.1
Caenorhabditis elegans (roundworm)	phb-2	NP_495250.2
Schizosaccharomyces pombe (fission yeast)	phb2	NP_588144.2
Arabidopsis thaliana (thale cress)	PHB6	NP_001077924.1
Arabidopsis thaliana (thale cress)	PHB7	NP_199227.1
Oryza sativa (rice)	Os03g0841700	NP_001051853.1
Pan troglodytes (chimpanzee)	PHB2	XP_508977.2
Danio rerio (zebrafish)	phb2a	NP_955975.1
Neurospora crassa	NCU03310	XP_964487.1
Oryza sativa (rice)	Os07g0262200	NP_001059325.1

Gene Ontology
Bibliography

Related articles in PubMed

Evolution of complexity in the zebrafish synapse proteome.
Bay?s ?, Collins MO, Reig-Viader R, Gou G, Goulding D, Izquierdo A, Choudhary JS, Emes RD, Grant SG
Nature communications814613(2017 Mar)

RFX transcription factors are essential for hearing in mice.
Elkon R, Milon B, Morrison L, Shah M, Vijayakumar S, Racherla M, Leitch CC, Silipino L, Hadi S, Weiss-Gayet M, Barras E, Schmid CD, Ait-Lounis A, Barnes A, Song Y, Eisenman DJ, Eliyahu E, Frolenkov GI, Strome SE, Durand B, Zaghloul NA, Jones SM, Reith W, Hertzano R
Nature communications68549(2015 Oct)

Chronic unpredictable stress (CUS)-induced anxiety and related mood disorders in a zebrafish model: altered brain proteome profile implicates mitochondrial dysfunction.
Chakravarty S, Reddy BR, Sudhakar SR, Saxena S, Das T, Meghah V, Brahmendra Swamy CV, Kumar A, Idris MM
PloS one8(5)e63302(2013)

The zebrafish gene map defines ancestral vertebrate chromosomes.
Woods IG, Wilson C, Friedlander B, Chang P, Reyes DK, Nix R, Kelly PD, Chu F, Postlethwait JH, Talbot WS
Genome research15(9)1307-14(2005 Sep)

Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences.
Strausberg RL, Feingold EA, Grouse LH, Derge JG, Klausner RD, Collins FS, Wagner L, Shenmen CM, Schuler GD, Altschul SF, Zeeberg B, Buetow KH, Schaefer CF, Bhat NK, Hopkins RF, Jordan H, Moore T, Max SI, Wang J, Hsieh F, Diatchenko L, Marusina K, Farmer AA, Rubin GM, Hong L, Stapleton M, Soares MB, Bonaldo MF, Casavant TL, Scheetz TE, Brownstein MJ, Usdin TB, Toshiyuki S, Carninci P, Prange C, Raha SS, Loquellano NA, Peters GJ, Abramson RD, Mullahy SJ, Bosak SA, McEwan PJ, McKernan KJ, Malek JA, Gunaratne PH, Richards S, Worley KC, Hale S, Garcia AM, Gay LJ, Hulyk SW, Villalon DK, Muzny DM, Sodergren EJ, Lu X, Gibbs RA, Fahey J, Helton E, Ketteman M, Madan A, Rodrigues S, Sanchez A, Whiting M, Madan A, Young AC, Shevchenko Y, Bouffard GG, Blakesley RW, Touchman JW, Green ED, Dickson MC, Rodriguez AC, Grimwood J, Schmutz J, Myers RM, Butterfield YS, Krzywinski MI, Skalska U, Smailus DE, Schnerch A, Schein JE, Jones SJ, Marra MA,
Proceedings of the National Academy of Sciences of the United States of America99(26)16899-903(2002 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following phb2a gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the phb2a cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODa39812	NM_199681.2 Latest version!	Danio rerio prohibitin 2a (phb2a), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$174.30 $249.00
ODa39812	XM_017357679.2 Latest version!	Danio rerio prohibitin 2a (phb2a), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$174.30 $249.00
View Old Accession Versions >>
ODa39812	XM_017357679.1	Danio rerio prohibitin 2a (phb2a), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$174.30 $249.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

phb2a ( NM_199681.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_955975.1 Danio rerio prohibitin 2a (phb2a), mRNA.

Price & Availability↑

CloneID	ODa39812
Clone ID Related Accession (Same CDS sequence)	XM_017357679.2 , XM_017357679.1 , NM_199681.2
Accession Version	NM_199681.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 909bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-01-26
Organism	Danio rerio(zebrafish)
Product	prohibitin 2a
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from CABZ01087191.1, EH546958.1 and CABZ01087190.1. On May 17, 2016 this sequence version replaced NM_199681.1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. ##Evidence-Data-START## Transcript exon combination :: GDQQ01003246.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA2168446, SAMEA2168447 [ECO:0000350] ##Evidence-Data-END##

ATGGCGAGCA AAGATCCCGG AAATTTTTTG CAGCAGCTCA GGCAGATTGC CAGCAGAATG 
GGCTCTGGTC CTCGAGGCGC TGGTTTGGGT GTGAAGCTGC TCATTGGAGC TGGAGCCCTC 
GCCTATGGGG TCAAAGAAGC CACATATACA GTGGAGGGTG GTCAGAGAGC CGTTATCTTC 
AGCAGAATCG GAGGGATGCA GATGGACACT GTACTTGCAG AAGGATTGCA CTTCAGGATG 
CCGTGGTTCC AGTATCCTAT TATCTATGAT ATCCGAGCCA GGCCCAGGAA GATTTCCTCT 
CTCACTGGAA GCAAAGATCT TCAGATGGTA AACATTGGTC TACGTGTGTT GTCTCGCCCG 
GTTGCCTCCC AATTGCCAAT CATGTACCAG CAGCTGGGGA AGGACTACGA TGAGCGTGTG 
CTTCCTTCCA TCGTCAACGA AGTGCTCAAG AGCGTCGTGG CCAAGTTTAA CGCCTCCCAG 
CTGATCACAC AGAGAGCTCA GGTGTCACTG CTCATAAGGC GAGATCTGAT CGAGAGGGCT 
AAAGACTTTA ACATCATCCT GGATGACGTG GCTATCACTG AGCTCAGCTT CAGCAAAGAA 
TACACAGCTG CTGTGGAGGC CAAACAAGTC GCCCAGCAGG AGGCGCAGAG GGCTCAGTTC 
TTCGTAGAGA AGGCCAAGCA GGACCAGAGA CAGAAGATTA TCCAGGCTGA AGGTGAAGCT 
GAGGCTGCTA AAATGTTGGG TCAAGCTGTT ACGAAGAACC CGGGTTACCT GAAACTCAGA 
CGCATCAGAG CAGCACAAAA TATTGCCAAA ACGGTGGCAG CTTCTCAGAA CAAGGTGTAC 
CTAAGTGCAG ACAGCCTGGT TCTGAACCTC CAGGACAGCT CCTTTAACAA CCTGTCACTG 
GGGAAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_955975.1
CDS	54..962
Translation	MASKDPGNFLQQLRQIASRMGSGPRGAGLGVKLLIGAGALAYGVKEATYTVEGGQRAVIFSRIGGMQMDTVLAEGLHFRMPWFQYPIIYDIRARPRKISSLTGSKDLQMVNIGLRVLSRPVASQLPIMYQQLGKDYDERVLPSIVNEVLKSVVAKFNASQLITQRAQVSLLIRRDLIERAKDFNIILDDVAITELSFSKEYTAAVEAKQVAQQEAQRAQFFVEKAKQDQRQKIIQAEGEAEAAKMLGQAVTKNPGYLKLRRIRAAQNIAKTVAASQNKVYLSADSLVLNLQDSSFNNLSLGK

Target ORF information:

RefSeq Version	NM_199681.2
Organism	Danio rerio(zebrafish)
Definition	Danio rerio prohibitin 2a (phb2a), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_199681.2

ORF Insert Sequence:

ATGGCGAGCA AAGATCCCGG AAATTTTTTG CAGCAGCTCA GGCAGATTGC CAGCAGAATG 
GGCTCTGGTC CTCGAGGCGC TGGTTTGGGT GTGAAGCTGC TCATTGGAGC TGGAGCCCTC 
GCCTATGGGG TCAAAGAAGC CACATATACA GTGGAGGGTG GTCAGAGAGC CGTTATCTTC 
AGCAGAATCG GAGGGATGCA GATGGACACT GTACTTGCAG AAGGATTGCA CTTCAGGATG 
CCGTGGTTCC AGTATCCTAT TATCTATGAT ATCCGAGCCA GGCCCAGGAA GATTTCCTCT 
CTCACTGGAA GCAAAGATCT TCAGATGGTA AACATTGGTC TACGTGTGTT GTCTCGCCCG 
GTTGCCTCCC AATTGCCAAT CATGTACCAG CAGCTGGGGA AGGACTACGA TGAGCGTGTG 
CTTCCTTCCA TCGTCAACGA AGTGCTCAAG AGCGTCGTGG CCAAGTTTAA CGCCTCCCAG 
CTGATCACAC AGAGAGCTCA GGTGTCACTG CTCATAAGGC GAGATCTGAT CGAGAGGGCT 
AAAGACTTTA ACATCATCCT GGATGACGTG GCTATCACTG AGCTCAGCTT CAGCAAAGAA 
TACACAGCTG CTGTGGAGGC CAAACAAGTC GCCCAGCAGG AGGCGCAGAG GGCTCAGTTC 
TTCGTAGAGA AGGCCAAGCA GGACCAGAGA CAGAAGATTA TCCAGGCTGA AGGTGAAGCT 
GAGGCTGCTA AAATGTTGGG TCAAGCTGTT ACGAAGAACC CGGGTTACCT GAAACTCAGA 
CGCATCAGAG CAGCACAAAA TATTGCCAAA ACGGTGGCAG CTTCTCAGAA CAAGGTGTAC 
CTAAGTGCAG ACAGCCTGGT TCTGAACCTC CAGGACAGCT CCTTTAACAA CCTGTCACTG 
GGGAAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

phb2a ( XM_017357679.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_017213168.1 Danio rerio prohibitin 2a (phb2a), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa39812
Clone ID Related Accession (Same CDS sequence)	XM_017357679.2 , XM_017357679.1 , NM_199681.2
Accession Version	XM_017357679.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 909bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	prohibitin 2a isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007113.7) and transcript sequence (BC059510.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_017357679.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## assembly gap :: added 41 transcript bases to patch genome assembly gap ##RefSeq-Attributes-END##

ATGGCGAGCA AAGATCCCGG AAATTTTTTG CAGCAGCTCA GGCAGATTGC CAGCAGAATG 
GGCTCTGGTC CTCGAGGCGC TGGTTTGGGT GTGAAGCTGC TCATTGGAGC TGGAGCCCTC 
GCCTATGGGG TCAAAGAAGC CACATATACA GTGGAGGGTG GTCAGAGAGC CGTTATCTTC 
AGCAGAATCG GAGGGATGCA GATGGACACT GTACTTGCAG AAGGATTGCA CTTCAGGATG 
CCGTGGTTCC AGTATCCTAT TATCTATGAT ATCCGAGCCA GGCCCAGGAA GATTTCCTCT 
CTCACTGGAA GCAAAGATCT TCAGATGGTA AACATTGGTC TACGTGTGTT GTCTCGCCCG 
GTTGCCTCCC AATTGCCAAT CATGTACCAG CAGCTGGGGA AGGACTACGA TGAGCGTGTG 
CTTCCTTCCA TCGTCAACGA AGTGCTCAAG AGCGTCGTGG CCAAGTTTAA CGCCTCCCAG 
CTGATCACAC AGAGAGCTCA GGTGTCACTG CTCATAAGGC GAGATCTGAT CGAGAGGGCT 
AAAGACTTTA ACATCATCCT GGATGACGTG GCTATCACTG AGCTCAGCTT CAGCAAAGAA 
TACACAGCTG CTGTGGAGGC CAAACAAGTC GCCCAGCAGG AGGCGCAGAG GGCTCAGTTC 
TTCGTAGAGA AGGCCAAGCA GGACCAGAGA CAGAAGATTA TCCAGGCTGA AGGTGAAGCT 
GAGGCTGCTA AAATGTTGGG TCAAGCTGTT ACGAAGAACC CGGGTTACCT GAAACTCAGA 
CGCATCAGAG CAGCACAAAA TATTGCCAAA ACGGTGGCAG CTTCTCAGAA CAAGGTGTAC 
CTAAGTGCAG ACAGCCTGGT TCTGAACCTC CAGGACAGCT CCTTTAACAA CCTGTCACTG 
GGGAAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017213168.1
CDS	79..987
Translation	MASKDPGNFLQQLRQIASRMGSGPRGAGLGVKLLIGAGALAYGVKEATYTVEGGQRAVIFSRIGGMQMDTVLAEGLHFRMPWFQYPIIYDIRARPRKISSLTGSKDLQMVNIGLRVLSRPVASQLPIMYQQLGKDYDERVLPSIVNEVLKSVVAKFNASQLITQRAQVSLLIRRDLIERAKDFNIILDDVAITELSFSKEYTAAVEAKQVAQQEAQRAQFFVEKAKQDQRQKIIQAEGEAEAAKMLGQAVTKNPGYLKLRRIRAAQNIAKTVAASQNKVYLSADSLVLNLQDSSFNNLSLGK

Target ORF information:

RefSeq Version	XM_017357679.2
Organism	Danio rerio(zebrafish)
Definition	Danio rerio prohibitin 2a (phb2a), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017357679.2

ORF Insert Sequence:

ATGGCGAGCA AAGATCCCGG AAATTTTTTG CAGCAGCTCA GGCAGATTGC CAGCAGAATG 
GGCTCTGGTC CTCGAGGCGC TGGTTTGGGT GTGAAGCTGC TCATTGGAGC TGGAGCCCTC 
GCCTATGGGG TCAAAGAAGC CACATATACA GTGGAGGGTG GTCAGAGAGC CGTTATCTTC 
AGCAGAATCG GAGGGATGCA GATGGACACT GTACTTGCAG AAGGATTGCA CTTCAGGATG 
CCGTGGTTCC AGTATCCTAT TATCTATGAT ATCCGAGCCA GGCCCAGGAA GATTTCCTCT 
CTCACTGGAA GCAAAGATCT TCAGATGGTA AACATTGGTC TACGTGTGTT GTCTCGCCCG 
GTTGCCTCCC AATTGCCAAT CATGTACCAG CAGCTGGGGA AGGACTACGA TGAGCGTGTG 
CTTCCTTCCA TCGTCAACGA AGTGCTCAAG AGCGTCGTGG CCAAGTTTAA CGCCTCCCAG 
CTGATCACAC AGAGAGCTCA GGTGTCACTG CTCATAAGGC GAGATCTGAT CGAGAGGGCT 
AAAGACTTTA ACATCATCCT GGATGACGTG GCTATCACTG AGCTCAGCTT CAGCAAAGAA 
TACACAGCTG CTGTGGAGGC CAAACAAGTC GCCCAGCAGG AGGCGCAGAG GGCTCAGTTC 
TTCGTAGAGA AGGCCAAGCA GGACCAGAGA CAGAAGATTA TCCAGGCTGA AGGTGAAGCT 
GAGGCTGCTA AAATGTTGGG TCAAGCTGTT ACGAAGAACC CGGGTTACCT GAAACTCAGA 
CGCATCAGAG CAGCACAAAA TATTGCCAAA ACGGTGGCAG CTTCTCAGAA CAAGGTGTAC 
CTAAGTGCAG ACAGCCTGGT TCTGAACCTC CAGGACAGCT CCTTTAACAA CCTGTCACTG 
GGGAAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

phb2a ( XM_017357679.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_017213168.1 Danio rerio prohibitin 2a (phb2a), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa39812
Clone ID Related Accession (Same CDS sequence)	XM_017357679.2 , XM_017357679.1 , NM_199681.2
Accession Version	XM_017357679.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 909bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	prohibitin 2a isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007113.6) and transcript sequence (BC059510.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## assembly gap :: added 41 transcript bases to patch genome assembly gap ##RefSeq-Attributes-END##

ATGGCGAGCA AAGATCCCGG AAATTTTTTG CAGCAGCTCA GGCAGATTGC CAGCAGAATG 
GGCTCTGGTC CTCGAGGCGC TGGTTTGGGT GTGAAGCTGC TCATTGGAGC TGGAGCCCTC 
GCCTATGGGG TCAAAGAAGC CACATATACA GTGGAGGGTG GTCAGAGAGC CGTTATCTTC 
AGCAGAATCG GAGGGATGCA GATGGACACT GTACTTGCAG AAGGATTGCA CTTCAGGATG 
CCGTGGTTCC AGTATCCTAT TATCTATGAT ATCCGAGCCA GGCCCAGGAA GATTTCCTCT 
CTCACTGGAA GCAAAGATCT TCAGATGGTA AACATTGGTC TACGTGTGTT GTCTCGCCCG 
GTTGCCTCCC AATTGCCAAT CATGTACCAG CAGCTGGGGA AGGACTACGA TGAGCGTGTG 
CTTCCTTCCA TCGTCAACGA AGTGCTCAAG AGCGTCGTGG CCAAGTTTAA CGCCTCCCAG 
CTGATCACAC AGAGAGCTCA GGTGTCACTG CTCATAAGGC GAGATCTGAT CGAGAGGGCT 
AAAGACTTTA ACATCATCCT GGATGACGTG GCTATCACTG AGCTCAGCTT CAGCAAAGAA 
TACACAGCTG CTGTGGAGGC CAAACAAGTC GCCCAGCAGG AGGCGCAGAG GGCTCAGTTC 
TTCGTAGAGA AGGCCAAGCA GGACCAGAGA CAGAAGATTA TCCAGGCTGA AGGTGAAGCT 
GAGGCTGCTA AAATGTTGGG TCAAGCTGTT ACGAAGAACC CGGGTTACCT GAAACTCAGA 
CGCATCAGAG CAGCACAAAA TATTGCCAAA ACGGTGGCAG CTTCTCAGAA CAAGGTGTAC 
CTAAGTGCAG ACAGCCTGGT TCTGAACCTC CAGGACAGCT CCTTTAACAA CCTGTCACTG 
GGGAAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017213168.1
CDS	66..974
Translation	MASKDPGNFLQQLRQIASRMGSGPRGAGLGVKLLIGAGALAYGVKEATYTVEGGQRAVIFSRIGGMQMDTVLAEGLHFRMPWFQYPIIYDIRARPRKISSLTGSKDLQMVNIGLRVLSRPVASQLPIMYQQLGKDYDERVLPSIVNEVLKSVVAKFNASQLITQRAQVSLLIRRDLIERAKDFNIILDDVAITELSFSKEYTAAVEAKQVAQQEAQRAQFFVEKAKQDQRQKIIQAEGEAEAAKMLGQAVTKNPGYLKLRRIRAAQNIAKTVAASQNKVYLSADSLVLNLQDSSFNNLSLGK

Target ORF information:

RefSeq Version	XM_017357679.1
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio prohibitin 2a (phb2a), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017357679.1

ORF Insert Sequence:

ATGGCGAGCA AAGATCCCGG AAATTTTTTG CAGCAGCTCA GGCAGATTGC CAGCAGAATG 
GGCTCTGGTC CTCGAGGCGC TGGTTTGGGT GTGAAGCTGC TCATTGGAGC TGGAGCCCTC 
GCCTATGGGG TCAAAGAAGC CACATATACA GTGGAGGGTG GTCAGAGAGC CGTTATCTTC 
AGCAGAATCG GAGGGATGCA GATGGACACT GTACTTGCAG AAGGATTGCA CTTCAGGATG 
CCGTGGTTCC AGTATCCTAT TATCTATGAT ATCCGAGCCA GGCCCAGGAA GATTTCCTCT 
CTCACTGGAA GCAAAGATCT TCAGATGGTA AACATTGGTC TACGTGTGTT GTCTCGCCCG 
GTTGCCTCCC AATTGCCAAT CATGTACCAG CAGCTGGGGA AGGACTACGA TGAGCGTGTG 
CTTCCTTCCA TCGTCAACGA AGTGCTCAAG AGCGTCGTGG CCAAGTTTAA CGCCTCCCAG 
CTGATCACAC AGAGAGCTCA GGTGTCACTG CTCATAAGGC GAGATCTGAT CGAGAGGGCT 
AAAGACTTTA ACATCATCCT GGATGACGTG GCTATCACTG AGCTCAGCTT CAGCAAAGAA 
TACACAGCTG CTGTGGAGGC CAAACAAGTC GCCCAGCAGG AGGCGCAGAG GGCTCAGTTC 
TTCGTAGAGA AGGCCAAGCA GGACCAGAGA CAGAAGATTA TCCAGGCTGA AGGTGAAGCT 
GAGGCTGCTA AAATGTTGGG TCAAGCTGTT ACGAAGAACC CGGGTTACCT GAAACTCAGA 
CGCATCAGAG CAGCACAAAA TATTGCCAAA ACGGTGGCAG CTTCTCAGAA CAAGGTGTAC 
CTAAGTGCAG ACAGCCTGGT TCTGAACCTC CAGGACAGCT CCTTTAACAA CCTGTCACTG 
GGGAAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Evolution of complexity in the zebrafish synapse proteome.
Nature communications814613(2017 Mar)
Bay?s ?,Collins MO,Reig-Viader R,Gou G,Goulding D,Izquierdo A,Choudhary JS,Emes RD,Grant SG

RFX transcription factors are essential for hearing in mice.
Nature communications68549(2015 Oct)
Elkon R,Milon B,Morrison L,Shah M,Vijayakumar S,Racherla M,Leitch CC,Silipino L,Hadi S,Weiss-Gayet M,Barras E,Schmid CD,Ait-Lounis A,Barnes A,Song Y,Eisenman DJ,Eliyahu E,Frolenkov GI,Strome SE,Durand B,Zaghloul NA,Jones SM,Reith W,Hertzano R

Chronic unpredictable stress (CUS)-induced anxiety and related mood disorders in a zebrafish model: altered brain proteome profile implicates mitochondrial dysfunction.
PloS one8(5)e63302(2013)
Chakravarty S,Reddy BR,Sudhakar SR,Saxena S,Das T,Meghah V,Brahmendra Swamy CV,Kumar A,Idris MM

The zebrafish gene map defines ancestral vertebrate chromosomes.
Genome research15(9)1307-14(2005 Sep)
Woods IG,Wilson C,Friedlander B,Chang P,Reyes DK,Nix R,Kelly PD,Chu F,Postlethwait JH,Talbot WS

Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences.
Proceedings of the National Academy of Sciences of the United States of America99(26)16899-903(2002 Dec)
Strausberg RL,Feingold EA,Grouse LH,Derge JG,Klausner RD,Collins FS,Wagner L,Shenmen CM,Schuler GD,Altschul SF,Zeeberg B,Buetow KH,Schaefer CF,Bhat NK,Hopkins RF,Jordan H,Moore T,Max SI,Wang J,Hsieh F,Diatchenko L,Marusina K,Farmer AA,Rubin GM,Hong L,Stapleton M,Soares MB,Bonaldo MF,Casavant TL,Scheetz TE,Brownstein MJ,Usdin TB,Toshiyuki S,Carninci P,Prange C,Raha SS,Loquellano NA,Peters GJ,Abramson RD,Mullahy SJ,Bosak SA,McEwan PJ,McKernan KJ,Malek JA,Gunaratne PH,Richards S,Worley KC,Hale S,Garcia AM,Gay LJ,Hulyk SW,Villalon DK,Muzny DM,Sodergren EJ,Lu X,Gibbs RA,Fahey J,Helton E,Ketteman M,Madan A,Rodrigues S,Sanchez A,Whiting M,Madan A,Young AC,Shevchenko Y,Bouffard GG,Blakesley RW,Touchman JW,Green ED,Dickson MC,Rodriguez AC,Grimwood J,Schmutz J,Myers RM,Butterfield YS,Krzywinski MI,Skalska U,Smailus DE,Schnerch A,Schein JE,Jones SJ,Marra MA,

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