ttpa cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Gene Symbol	ttpa
Entrez Gene ID	325906
Full Name	tocopherol (alpha) transfer protein
Synonyms	wu:fd44e05,zgc:66361
General protein information	Preferred Names tocopherol (alpha) transfer protein Names alpha-tocopherol transfer protein tocopherol (alpha) transfer protein (ataxia (Friedreich-like) with vitamin E deficiency)
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA Protein Name

NM_199731.2 NP_956025.2 alpha-tocopherol transfer protein
Pathways from BioSystems

mRNA	Protein	Name
NM_199731.2	NP_956025.2	alpha-tocopherol transfer protein

Homologies

Pan troglodytes (chimpanzee)	TTPA	XP_528150.2
Rattus norvegicus (Norway rat)	Ttpa	NP_037180.1
Gallus gallus (chicken)	TTPA	XP_001234376.2
Bos taurus (cattle)	TTPA	NP_001193605.1
Danio rerio (zebrafish)	ttpa	NP_956025.2
	TTPA	XP_001092014.1
Mus musculus (house mouse)	Ttpa	NP_056582.1
Homo sapiens (human)	TTPA	NP_000361.1
Canis lupus familiaris (dog)	TTPA	XP_005638207.1

Gene Ontology
Bibliography

Related articles in PubMed

RFX transcription factors are essential for hearing in mice.
Elkon R, Milon B, Morrison L, Shah M, Vijayakumar S, Racherla M, Leitch CC, Silipino L, Hadi S, Weiss-Gayet M, Barras E, Schmid CD, Ait-Lounis A, Barnes A, Song Y, Eisenman DJ, Eliyahu E, Frolenkov GI, Strome SE, Durand B, Zaghloul NA, Jones SM, Reith W, Hertzano R
Nature communications68549(2015 Oct)

Zebrafish (Danio rerio) fed vitamin E-deficient diets produce embryos with increased morphologic abnormalities and mortality.
Miller GW, Labut EM, Lebold KM, Floeter A, Tanguay RL, Traber MG
The Journal of nutritional biochemistry23(5)478-86(2012 May)

The ?-tocopherol transfer protein is essential for vertebrate embryogenesis.
Miller GW, Ulatowski L, Labut EM, Lebold KM, Manor D, Atkinson J, Barton CL, Tanguay RL, Traber MG
PloS one7(10)e47402(2012)

Hepatic gene expression profiling using Genechips in zebrafish exposed to 17alpha-ethynylestradiol.
Hoffmann JL, Torontali SP, Thomason RG, Lee DM, Brill JL, Price BB, Carr GJ, Versteeg DJ
Aquatic toxicology (Amsterdam, Netherlands)79(3)233-46(2006 Sep)

Hematopoietic gene expression profile in zebrafish kidney marrow.
Song HD, Sun XJ, Deng M, Zhang GW, Zhou Y, Wu XY, Sheng Y, Chen Y, Ruan Z, Jiang CL, Fan HY, Zon LI, Kanki JP, Liu TX, Look AT, Chen Z
Proceedings of the National Academy of Sciences of the United States of America101(46)16240-5(2004 Nov)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

We conclude that TTP is essential for early development of the vertebrate central nervous system.
Title: The ?-tocopherol transfer protein is essential for vertebrate embryogenesis.

The following ttpa gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ttpa cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ODa31241	NM_199731.2 Latest version!	Danio rerio tocopherol (alpha) transfer protein (ttpa), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$174.30 $249.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

ttpa ( NM_199731.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_956025.2 Danio rerio tocopherol (alpha) transfer protein (ttpa), mRNA.

Price & Availability↑

CloneID	ODa31241
Clone ID Related Accession (Same CDS sequence)	NM_199731.2
Accession Version	NM_199731.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 858bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-11-23
Organism	Danio rerio(zebrafish)
Product	alpha-tocopherol transfer protein
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BX248497.11. On Feb 4, 2011 this sequence version replaced NM_199731.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## Transcript exon combination :: GDQH01015346.1, GFIL01042243.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2168446, SAMEA2168447 [ECO:0000348] ##Evidence-Data-END##

ATGAAGTCCG AAGAAGTAGA CGAGACTGAA GAATTAAACA ATTTACCTGT TGATTCCTCC 
CGGATTGCAC CATATTTGTC AGAGTTGAAA GAAAAAGCAG AGGCAGAATT GCGCATTCGA 
GACTTGGACT TGTCGAAAAC TTTTTTGATA CGGTTTTTGC AGGCGAGAGA CTTTGATGTG 
GCGCTGGCGC TCAAGCTGTT GATTAACTAT CATAAATGGA GACAAGAATG TCCAGAAATA 
ACAGCCGATC TGCGTCCCTC ATCCGTCATT GGACTTCTCC AAAACAACTA CCATGGAGTT 
TTGAGATCGA GAGATGATGC TGGAAGTCGA GTTTTAATCT ATCGGATTGG TAAATGGAAC 
CCCAAAGAGT TCACAGCCTA TGAGGTTTTT CGTGTCAGCC TCATTACGTC AGAGCTGATA 
GTTCAAGAAT GGGAGACGCA AAGAAATGGA CTCAAAGCGA TTTTTGATCT CCAAGATTGG 
TGTTTTGCTC ATGCTCTGCA AATAAATCCT TCTTTGGCAA AAAAGATATC CTCTGTTCTT 
ACGGATTCCT TTCCATTGAA AGTGCGTGGC ATCCACTTGA TAAACGAGCC AATTTTTTTC 
CGTCCTGTCT TTGCTATGAT CCGACCATTT CTTCCAGACA AAATCAAACA ACGGATTCAT 
ATGCATGGCT GTTCATATGC CCGAAGTCTC TGCAATTACT TTCCAAAGGC AGTACTCCCT 
CCTGTGTATG GAGGAACTGG ACCCAGCGTA GATGAAGTGT GTCAAGAGTG GACCGAGTAC 
ATCATGCAGT CTGAAGACTA TCTGCACAGG CTCTCTGTTG ACTTAGGTGG AGAAGGAGGA 
CATGCTTCTC AGTCATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_956025.2
CDS	75..932
Translation	MKSEEVDETEELNNLPVDSSRIAPYLSELKEKAEAELRIRDLDLSKTFLIRFLQARDFDVALALKLLINYHKWRQECPEITADLRPSSVIGLLQNNYHGVLRSRDDAGSRVLIYRIGKWNPKEFTAYEVFRVSLITSELIVQEWETQRNGLKAIFDLQDWCFAHALQINPSLAKKISSVLTDSFPLKVRGIHLINEPIFFRPVFAMIRPFLPDKIKQRIHMHGCSYARSLCNYFPKAVLPPVYGGTGPSVDEVCQEWTEYIMQSEDYLHRLSVDLGGEGGHASQS

Target ORF information:

RefSeq Version	NM_199731.2
Organism	Danio rerio(zebrafish)
Definition	Danio rerio tocopherol (alpha) transfer protein (ttpa), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_199731.2

ORF Insert Sequence:

ATGAAGTCCG AAGAAGTAGA CGAGACTGAA GAATTAAACA ATTTACCTGT TGATTCCTCC 
CGGATTGCAC CATATTTGTC AGAGTTGAAA GAAAAAGCAG AGGCAGAATT GCGCATTCGA 
GACTTGGACT TGTCGAAAAC TTTTTTGATA CGGTTTTTGC AGGCGAGAGA CTTTGATGTG 
GCGCTGGCGC TCAAGCTGTT GATTAACTAT CATAAATGGA GACAAGAATG TCCAGAAATA 
ACAGCCGATC TGCGTCCCTC ATCCGTCATT GGACTTCTCC AAAACAACTA CCATGGAGTT 
TTGAGATCGA GAGATGATGC TGGAAGTCGA GTTTTAATCT ATCGGATTGG TAAATGGAAC 
CCCAAAGAGT TCACAGCCTA TGAGGTTTTT CGTGTCAGCC TCATTACGTC AGAGCTGATA 
GTTCAAGAAT GGGAGACGCA AAGAAATGGA CTCAAAGCGA TTTTTGATCT CCAAGATTGG 
TGTTTTGCTC ATGCTCTGCA AATAAATCCT TCTTTGGCAA AAAAGATATC CTCTGTTCTT 
ACGGATTCCT TTCCATTGAA AGTGCGTGGC ATCCACTTGA TAAACGAGCC AATTTTTTTC 
CGTCCTGTCT TTGCTATGAT CCGACCATTT CTTCCAGACA AAATCAAACA ACGGATTCAT 
ATGCATGGCT GTTCATATGC CCGAAGTCTC TGCAATTACT TTCCAAAGGC AGTACTCCCT 
CCTGTGTATG GAGGAACTGG ACCCAGCGTA GATGAAGTGT GTCAAGAGTG GACCGAGTAC 
ATCATGCAGT CTGAAGACTA TCTGCACAGG CTCTCTGTTG ACTTAGGTGG AGAAGGAGGA 
CATGCTTCTC AGTCATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

RFX transcription factors are essential for hearing in mice.
Nature communications68549(2015 Oct)
Elkon R,Milon B,Morrison L,Shah M,Vijayakumar S,Racherla M,Leitch CC,Silipino L,Hadi S,Weiss-Gayet M,Barras E,Schmid CD,Ait-Lounis A,Barnes A,Song Y,Eisenman DJ,Eliyahu E,Frolenkov GI,Strome SE,Durand B,Zaghloul NA,Jones SM,Reith W,Hertzano R

Zebrafish (Danio rerio) fed vitamin E-deficient diets produce embryos with increased morphologic abnormalities and mortality.
The Journal of nutritional biochemistry23(5)478-86(2012 May)
Miller GW,Labut EM,Lebold KM,Floeter A,Tanguay RL,Traber MG

The ?-tocopherol transfer protein is essential for vertebrate embryogenesis.
PloS one7(10)e47402(2012)
Miller GW,Ulatowski L,Labut EM,Lebold KM,Manor D,Atkinson J,Barton CL,Tanguay RL,Traber MG

Hepatic gene expression profiling using Genechips in zebrafish exposed to 17alpha-ethynylestradiol.
Aquatic toxicology (Amsterdam, Netherlands)79(3)233-46(2006 Sep)
Hoffmann JL,Torontali SP,Thomason RG,Lee DM,Brill JL,Price BB,Carr GJ,Versteeg DJ

Hematopoietic gene expression profile in zebrafish kidney marrow.
Proceedings of the National Academy of Sciences of the United States of America101(46)16240-5(2004 Nov)
Song HD,Sun XJ,Deng M,Zhang GW,Zhou Y,Wu XY,Sheng Y,Chen Y,Ruan Z,Jiang CL,Fan HY,Zon LI,Kanki JP,Liu TX,Look AT,Chen Z

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ttpa cDNA ORF clone, Danio rerio(zebrafish)

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Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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