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srsf1a cDNA ORF clone, Danio rerio(zebrafish)

Summary

Gene Symbol srsf1a
Entrez Gene ID 406288
Full Name serine/arginine-rich splicing factor 1a
Synonyms sfrs1a,sfrs1l,wu:fb52g10,wu:fb97g12,zgc:66146
General protein information
Preferred Names

serine/arginine-rich splicing factor 1a

Names

serine/arginine-rich splicing factor 1A
splicing factor, arginine/serine-rich 1, like
splicing factor, arginine/serine-rich 1-like protein 1
splicing factor, arginine/serine-rich 1a
Gene Type protein-coding
Organism Danio rerio(zebrafish)

Reference Sequences (RefSeq)

mRNA and Protein(s)

mRNA Protein Name
XM_005157414.3 XP_005157471.1 serine/arginine-rich splicing factor 1A isoform X1
XM_005157415.3 XP_005157472.1 serine/arginine-rich splicing factor 1A isoform X1
XM_005157415.4 XP_005157472.1 serine/arginine-rich splicing factor 1A isoform X1
XM_005157414.4 XP_005157471.1 serine/arginine-rich splicing factor 1A isoform X1
NM_213015.1 NP_998180.1 serine/arginine-rich splicing factor 1A

Pathways from BioSystems


Homologies


Pan troglodytes (chimpanzee) SRSF1 NP_001182476.1
Gallus gallus (chicken) SRSF1 NP_001107213.1
Xenopus tropicalis (tropical clawed frog) srsf1 NP_001006919.1
Homo sapiens (human) SRSF1 NP_008855.1
Danio rerio (zebrafish) srsf1a NP_998180.1
Drosophila melanogaster (fruit fly) SF2 NP_652611.1
Mus musculus (house mouse) Srsf1 NP_775550.2
Arabidopsis thaliana (thale cress) SR1 NP_850934.1
Canis lupus familiaris (dog) SRSF1 XP_005624772.1
Bos taurus (cattle) SRSF1 NP_001069862.1
SRSF1 XP_001103473.2
Rattus norvegicus (Norway rat) Srsf1 NP_001103022.1
Anopheles gambiae (African malaria mosquito) AgaP_AGAP010496 XP_314469.4
Caenorhabditis elegans (roundworm) rsp-3 NP_499649.2
Arabidopsis thaliana (thale cress) AT4G02430 NP_567235.3

Gene Ontology


Bibliography

Related articles in PubMed

Number of inadvertent RNA targets for morpholino knockdown in Danio rerio is largely underestimated: evidence from the study of Ser/Arg-rich splicing factors.
Joris M, Schloesser M, Baurain D, Hanikenne M, Muller M, Motte P
Nucleic acids research45(16)9547-9557(2017 Sep)

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
Pasquier J, Cabau C, Nguyen T, Jouanno E, Severac D, Braasch I, Journot L, Pontarotti P, Klopp C, Postlethwait JH, Guiguen Y, Bobe J
BMC genomics17368(2016 05)

Genome wide analysis reveals Zic3 interaction with distal regulatory elements of stage specific developmental genes in zebrafish.
Winata CL, Kondrychyn I, Kumar V, Srinivasan KG, Orlov Y, Ravishankar A, Prabhakar S, Stanton LW, Korzh V, Mathavan S
PLoS genetics9(10)e1003852(2013 Oct)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following srsf1a gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the srsf1a cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
ODa00342 NM_213015.1
Latest version!		 Danio rerio serine and arginine rich splicing factor 1a (srsf1a), mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $174.30
$249.00
ODa20305 XM_005157414.4
Latest version!		 Danio rerio serine/arginine-rich splicing factor 1a (srsf1a), transcript variant X1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $174.30
$249.00
ODa20305 XM_005157415.4
Latest version!		 Danio rerio serine/arginine-rich splicing factor 1a (srsf1a), transcript variant X2, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $174.30
$249.00
View Old Accession Versions >>
ODa20305 XM_005157415.3 Danio rerio serine/arginine-rich splicing factor 1a (srsf1a), transcript variant X2, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $174.30
$249.00
ODa20305 XM_005157414.3 Danio rerio serine/arginine-rich splicing factor 1a (srsf1a), transcript variant X1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $174.30
$249.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)


CloneID ODa00342
Clone ID Related Accession (Same CDS sequence) NM_213015.1
Accession Version NM_213015.1 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 738bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2019-04-07
Organism Danio rerio(zebrafish)
Product serine/arginine-rich splicing factor 1A
Comment Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC055511.1. Sequence Note: This sequence has been modified as follows: removed 32 bp suspected to be vector contamination from the 5' end. ##Evidence-Data-START## Transcript exon combination :: BC055511.1 [ECO:0000332] ##Evidence-Data-END## 

1
61
121
181
241
301
361
421
481
541
601
661
721
ATGTCCGGTG GTGTGATCCG CGGCCCTGCT GGAAGCAACG ATTGCCGCAT CTATGTGGGA 
AACCTGCCGC CCGATATTCG CACCAAAGAT GTCGAAGATG TGTTTTATAA GTACGGAGCC 
ATCCGAGACA TCGATCTGAA AAACCGACGA GGCGGACCTC CATTCGCCTT TGTCGAGTTT 
GAAGACCCCA GAGATGCAGA GGATGCTGTT TATGCTCGTG ATGGCTATGA CTATGATGGC 
TATCGACTTC GAGTGGAATT TCCTAGGAGT GGACGAGGAA TGGGCAGAGG AGGTTTTGGT 
GGGGGTGGTG GAGGCGGTGG CGGCGGAGGA GGAGGAGGTG GTGGTGGGGC TCCTAGAGGT 
AGATATGGAC CCCCATCCAG ACGTTCAGAG TACAGAGTAA TTGTTTCAGG ACTTCCACCA 
AGCGGCAGCT GGCAGGATCT GAAGGATCAC ATGCGTGAAG CAGGTGATGT ATGTTACGCT 
GACGTTTTCC GAGATGGAAC TGGTGTGGTG GAGTTTGTTC GCAAAGAGGA CATGACCTAC 
GCCGTTCGCA AACTGGATAA CACAAAATTC CGGTCTCATG AGGGAGAAAC TGCGTACATC 
CGCGTAAAGG TGGACGGACC CCGCAGTCCA AGCTATGGAA GATCAAGGTC AAGGAGCCGC 
AGTCGCAGCA GGAGTCGCAG CCGCAGCAAC AACCGTAGCC GAAGCTACTC TCCACGCCGC 
AGCCGAGGAT CCCCTTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_998180.1
CDS48..785
Translation

Target ORF information:

RefSeq Version NM_213015.1
Organism Danio rerio(zebrafish)
Definition Danio rerio serine and arginine rich splicing factor 1a (srsf1a), mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 NM_213015.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
ATGTCCGGTG GTGTGATCCG CGGCCCTGCT GGAAGCAACG ATTGCCGCAT CTATGTGGGA 
AACCTGCCGC CCGATATTCG CACCAAAGAT GTCGAAGATG TGTTTTATAA GTACGGAGCC 
ATCCGAGACA TCGATCTGAA AAACCGACGA GGCGGACCTC CATTCGCCTT TGTCGAGTTT 
GAAGACCCCA GAGATGCAGA GGATGCTGTT TATGCTCGTG ATGGCTATGA CTATGATGGC 
TATCGACTTC GAGTGGAATT TCCTAGGAGT GGACGAGGAA TGGGCAGAGG AGGTTTTGGT 
GGGGGTGGTG GAGGCGGTGG CGGCGGAGGA GGAGGAGGTG GTGGTGGGGC TCCTAGAGGT 
AGATATGGAC CCCCATCCAG ACGTTCAGAG TACAGAGTAA TTGTTTCAGG ACTTCCACCA 
AGCGGCAGCT GGCAGGATCT GAAGGATCAC ATGCGTGAAG CAGGTGATGT ATGTTACGCT 
GACGTTTTCC GAGATGGAAC TGGTGTGGTG GAGTTTGTTC GCAAAGAGGA CATGACCTAC 
GCCGTTCGCA AACTGGATAA CACAAAATTC CGGTCTCATG AGGGAGAAAC TGCGTACATC 
CGCGTAAAGG TGGACGGACC CCGCAGTCCA AGCTATGGAA GATCAAGGTC AAGGAGCCGC 
AGTCGCAGCA GGAGTCGCAG CCGCAGCAAC AACCGTAGCC GAAGCTACTC TCCACGCCGC 
AGCCGAGGAT CCCCTTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID ODa20305
Clone ID Related Accession (Same CDS sequence) XM_005157414.3 , XM_005157415.3 , XM_005157415.4 , XM_005157414.4
Accession Version XM_005157414.3 Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 774bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2016-06-23
Organism Danio rerio(Zebrafish)
Product serine/arginine-rich splicing factor 1A isoform X1
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007126.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005157414.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

1
61
121
181
241
301
361
421
481
541
601
661
721
ATGTCCGGTG GTGTGATCCG CGGCCCTGCT GGAAGCAACG ATTGCCGCAT CTATGTGGGA 
AACCTGCCGC CCGATATTCG CACCAAAGAT GTCGAAGATG TGTTTTATAA GTACGGAGCC 
ATCCGAGACA TCGATCTGAA AAACCGACGA GGCGGACCTC CATTCGCCTT TGTCGAGTTT 
GAAGACCCCA GAGATGCAGA GGATGCTGTT TATGCTCGTG ATGGCTATGA CTATGATGGC 
TATCGACTTC GAGTGGAATT TCCTAGGAGT GGACGAGGAA TGGGCAGAGG AGGTTTTGGT 
GGGGGTGGTG GAGGCGGTGG CGGCGGAGGA GGAGGTGGTG GTGGGGCTCC TAGAGGTAGA 
TATGGACCCC CATCCAGACG TTCAGAGTAC AGAGTAATTG TTTCAGGACT TCCACCAAGC 
GGCAGCTGGC AGGATCTGAA GGATCACATG CGTGAAGCAG GTGATGTATG TTACGCTGAC 
GTTTTCCGAG ATGGAACTGG TGTGGTGGAG TTTGTTCGCA AAGAGGACAT GACCTACGCC 
GTTCGCAAAC TGGATAACAC AAAATTCCGG TCCCATGAGG GAGAAACTGC GTACATCCGC 
GTAAAGGTGG ACGGACCCCG CAGTCCAAGC TATGGAAGAT CAAGGTCAAG GAGCCGCAGT 
CGCAGCAGGA GTCGCAGCCG CAGCAACAAC CGTAGCCGAA GCTACTCTCC ACGCCGCAGC 
CGAGGATCCC CGCAGTACTC GCCACGTCAT AGCCGTTCCC GCTCTCGCTC CTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_005157471.1
CDS138..911
Translation

Target ORF information:

RefSeq Version XM_005157414.3
Organism Danio rerio(Zebrafish)
Definition Danio rerio serine/arginine-rich splicing factor 1a (srsf1a), transcript variant X1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 XM_005157414.3

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
ATGTCCGGTG GTGTGATCCG CGGCCCTGCT GGAAGCAACG ATTGCCGCAT CTATGTGGGA 
AACCTGCCGC CCGATATTCG CACCAAAGAT GTCGAAGATG TGTTTTATAA GTACGGAGCC 
ATCCGAGACA TCGATCTGAA AAACCGACGA GGCGGACCTC CATTCGCCTT TGTCGAGTTT 
GAAGACCCCA GAGATGCAGA GGATGCTGTT TATGCTCGTG ATGGCTATGA CTATGATGGC 
TATCGACTTC GAGTGGAATT TCCTAGGAGT GGACGAGGAA TGGGCAGAGG AGGTTTTGGT 
GGGGGTGGTG GAGGCGGTGG CGGCGGAGGA GGAGGTGGTG GTGGGGCTCC TAGAGGTAGA 
TATGGACCCC CATCCAGACG TTCAGAGTAC AGAGTAATTG TTTCAGGACT TCCACCAAGC 
GGCAGCTGGC AGGATCTGAA GGATCACATG CGTGAAGCAG GTGATGTATG TTACGCTGAC 
GTTTTCCGAG ATGGAACTGG TGTGGTGGAG TTTGTTCGCA AAGAGGACAT GACCTACGCC 
GTTCGCAAAC TGGATAACAC AAAATTCCGG TCCCATGAGG GAGAAACTGC GTACATCCGC 
GTAAAGGTGG ACGGACCCCG CAGTCCAAGC TATGGAAGAT CAAGGTCAAG GAGCCGCAGT 
CGCAGCAGGA GTCGCAGCCG CAGCAACAAC CGTAGCCGAA GCTACTCTCC ACGCCGCAGC 
CGAGGATCCC CGCAGTACTC GCCACGTCAT AGCCGTTCCC GCTCTCGCTC CTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID ODa20305
Clone ID Related Accession (Same CDS sequence) XM_005157414.3 , XM_005157415.3 , XM_005157415.4 , XM_005157414.4
Accession Version XM_005157415.3 Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 774bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2016-06-23
Organism Danio rerio(Zebrafish)
Product serine/arginine-rich splicing factor 1A isoform X1
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007126.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005157415.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

1
61
121
181
241
301
361
421
481
541
601
661
721
ATGTCCGGTG GTGTGATCCG CGGCCCTGCT GGAAGCAACG ATTGCCGCAT CTATGTGGGA 
AACCTGCCGC CCGATATTCG CACCAAAGAT GTCGAAGATG TGTTTTATAA GTACGGAGCC 
ATCCGAGACA TCGATCTGAA AAACCGACGA GGCGGACCTC CATTCGCCTT TGTCGAGTTT 
GAAGACCCCA GAGATGCAGA GGATGCTGTT TATGCTCGTG ATGGCTATGA CTATGATGGC 
TATCGACTTC GAGTGGAATT TCCTAGGAGT GGACGAGGAA TGGGCAGAGG AGGTTTTGGT 
GGGGGTGGTG GAGGCGGTGG CGGCGGAGGA GGAGGTGGTG GTGGGGCTCC TAGAGGTAGA 
TATGGACCCC CATCCAGACG TTCAGAGTAC AGAGTAATTG TTTCAGGACT TCCACCAAGC 
GGCAGCTGGC AGGATCTGAA GGATCACATG CGTGAAGCAG GTGATGTATG TTACGCTGAC 
GTTTTCCGAG ATGGAACTGG TGTGGTGGAG TTTGTTCGCA AAGAGGACAT GACCTACGCC 
GTTCGCAAAC TGGATAACAC AAAATTCCGG TCCCATGAGG GAGAAACTGC GTACATCCGC 
GTAAAGGTGG ACGGACCCCG CAGTCCAAGC TATGGAAGAT CAAGGTCAAG GAGCCGCAGT 
CGCAGCAGGA GTCGCAGCCG CAGCAACAAC CGTAGCCGAA GCTACTCTCC ACGCCGCAGC 
CGAGGATCCC CGCAGTACTC GCCACGTCAT AGCCGTTCCC GCTCTCGCTC CTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_005157472.1
CDS187..960
Translation

Target ORF information:

RefSeq Version XM_005157415.3
Organism Danio rerio(Zebrafish)
Definition Danio rerio serine/arginine-rich splicing factor 1a (srsf1a), transcript variant X2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 XM_005157415.3

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
ATGTCCGGTG GTGTGATCCG CGGCCCTGCT GGAAGCAACG ATTGCCGCAT CTATGTGGGA 
AACCTGCCGC CCGATATTCG CACCAAAGAT GTCGAAGATG TGTTTTATAA GTACGGAGCC 
ATCCGAGACA TCGATCTGAA AAACCGACGA GGCGGACCTC CATTCGCCTT TGTCGAGTTT 
GAAGACCCCA GAGATGCAGA GGATGCTGTT TATGCTCGTG ATGGCTATGA CTATGATGGC 
TATCGACTTC GAGTGGAATT TCCTAGGAGT GGACGAGGAA TGGGCAGAGG AGGTTTTGGT 
GGGGGTGGTG GAGGCGGTGG CGGCGGAGGA GGAGGTGGTG GTGGGGCTCC TAGAGGTAGA 
TATGGACCCC CATCCAGACG TTCAGAGTAC AGAGTAATTG TTTCAGGACT TCCACCAAGC 
GGCAGCTGGC AGGATCTGAA GGATCACATG CGTGAAGCAG GTGATGTATG TTACGCTGAC 
GTTTTCCGAG ATGGAACTGG TGTGGTGGAG TTTGTTCGCA AAGAGGACAT GACCTACGCC 
GTTCGCAAAC TGGATAACAC AAAATTCCGG TCCCATGAGG GAGAAACTGC GTACATCCGC 
GTAAAGGTGG ACGGACCCCG CAGTCCAAGC TATGGAAGAT CAAGGTCAAG GAGCCGCAGT 
CGCAGCAGGA GTCGCAGCCG CAGCAACAAC CGTAGCCGAA GCTACTCTCC ACGCCGCAGC 
CGAGGATCCC CGCAGTACTC GCCACGTCAT AGCCGTTCCC GCTCTCGCTC CTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID ODa20305
Clone ID Related Accession (Same CDS sequence) XM_005157414.3 , XM_005157415.3 , XM_005157415.4 , XM_005157414.4
Accession Version XM_005157415.4 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 774bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2017-06-14
Organism Danio rerio(zebrafish)
Product serine/arginine-rich splicing factor 1A isoform X1
Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007126.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005157415.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

1
61
121
181
241
301
361
421
481
541
601
661
721
ATGTCCGGTG GTGTGATCCG CGGCCCTGCT GGAAGCAACG ATTGCCGCAT CTATGTGGGA 
AACCTGCCGC CCGATATTCG CACCAAAGAT GTCGAAGATG TGTTTTATAA GTACGGAGCC 
ATCCGAGACA TCGATCTGAA AAACCGACGA GGCGGACCTC CATTCGCCTT TGTCGAGTTT 
GAAGACCCCA GAGATGCAGA GGATGCTGTT TATGCTCGTG ATGGCTATGA CTATGATGGC 
TATCGACTTC GAGTGGAATT TCCTAGGAGT GGACGAGGAA TGGGCAGAGG AGGTTTTGGT 
GGGGGTGGTG GAGGCGGTGG CGGCGGAGGA GGAGGTGGTG GTGGGGCTCC TAGAGGTAGA 
TATGGACCCC CATCCAGACG TTCAGAGTAC AGAGTAATTG TTTCAGGACT TCCACCAAGC 
GGCAGCTGGC AGGATCTGAA GGATCACATG CGTGAAGCAG GTGATGTATG TTACGCTGAC 
GTTTTCCGAG ATGGAACTGG TGTGGTGGAG TTTGTTCGCA AAGAGGACAT GACCTACGCC 
GTTCGCAAAC TGGATAACAC AAAATTCCGG TCCCATGAGG GAGAAACTGC GTACATCCGC 
GTAAAGGTGG ACGGACCCCG CAGTCCAAGC TATGGAAGAT CAAGGTCAAG GAGCCGCAGT 
CGCAGCAGGA GTCGCAGCCG CAGCAACAAC CGTAGCCGAA GCTACTCTCC ACGCCGCAGC 
CGAGGATCCC CGCAGTACTC GCCACGTCAT AGCCGTTCCC GCTCTCGCTC CTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_005157472.1
CDS187..960
Translation

Target ORF information:

RefSeq Version XM_005157415.4
Organism Danio rerio(zebrafish)
Definition Danio rerio serine/arginine-rich splicing factor 1a (srsf1a), transcript variant X2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 XM_005157415.4

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
ATGTCCGGTG GTGTGATCCG CGGCCCTGCT GGAAGCAACG ATTGCCGCAT CTATGTGGGA 
AACCTGCCGC CCGATATTCG CACCAAAGAT GTCGAAGATG TGTTTTATAA GTACGGAGCC 
ATCCGAGACA TCGATCTGAA AAACCGACGA GGCGGACCTC CATTCGCCTT TGTCGAGTTT 
GAAGACCCCA GAGATGCAGA GGATGCTGTT TATGCTCGTG ATGGCTATGA CTATGATGGC 
TATCGACTTC GAGTGGAATT TCCTAGGAGT GGACGAGGAA TGGGCAGAGG AGGTTTTGGT 
GGGGGTGGTG GAGGCGGTGG CGGCGGAGGA GGAGGTGGTG GTGGGGCTCC TAGAGGTAGA 
TATGGACCCC CATCCAGACG TTCAGAGTAC AGAGTAATTG TTTCAGGACT TCCACCAAGC 
GGCAGCTGGC AGGATCTGAA GGATCACATG CGTGAAGCAG GTGATGTATG TTACGCTGAC 
GTTTTCCGAG ATGGAACTGG TGTGGTGGAG TTTGTTCGCA AAGAGGACAT GACCTACGCC 
GTTCGCAAAC TGGATAACAC AAAATTCCGG TCCCATGAGG GAGAAACTGC GTACATCCGC 
GTAAAGGTGG ACGGACCCCG CAGTCCAAGC TATGGAAGAT CAAGGTCAAG GAGCCGCAGT 
CGCAGCAGGA GTCGCAGCCG CAGCAACAAC CGTAGCCGAA GCTACTCTCC ACGCCGCAGC 
CGAGGATCCC CGCAGTACTC GCCACGTCAT AGCCGTTCCC GCTCTCGCTC CTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID ODa20305
Clone ID Related Accession (Same CDS sequence) XM_005157414.3 , XM_005157415.3 , XM_005157415.4 , XM_005157414.4
Accession Version XM_005157414.4 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 774bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2017-06-14
Organism Danio rerio(zebrafish)
Product serine/arginine-rich splicing factor 1A isoform X1
Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007126.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005157414.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

1
61
121
181
241
301
361
421
481
541
601
661
721
ATGTCCGGTG GTGTGATCCG CGGCCCTGCT GGAAGCAACG ATTGCCGCAT CTATGTGGGA 
AACCTGCCGC CCGATATTCG CACCAAAGAT GTCGAAGATG TGTTTTATAA GTACGGAGCC 
ATCCGAGACA TCGATCTGAA AAACCGACGA GGCGGACCTC CATTCGCCTT TGTCGAGTTT 
GAAGACCCCA GAGATGCAGA GGATGCTGTT TATGCTCGTG ATGGCTATGA CTATGATGGC 
TATCGACTTC GAGTGGAATT TCCTAGGAGT GGACGAGGAA TGGGCAGAGG AGGTTTTGGT 
GGGGGTGGTG GAGGCGGTGG CGGCGGAGGA GGAGGTGGTG GTGGGGCTCC TAGAGGTAGA 
TATGGACCCC CATCCAGACG TTCAGAGTAC AGAGTAATTG TTTCAGGACT TCCACCAAGC 
GGCAGCTGGC AGGATCTGAA GGATCACATG CGTGAAGCAG GTGATGTATG TTACGCTGAC 
GTTTTCCGAG ATGGAACTGG TGTGGTGGAG TTTGTTCGCA AAGAGGACAT GACCTACGCC 
GTTCGCAAAC TGGATAACAC AAAATTCCGG TCCCATGAGG GAGAAACTGC GTACATCCGC 
GTAAAGGTGG ACGGACCCCG CAGTCCAAGC TATGGAAGAT CAAGGTCAAG GAGCCGCAGT 
CGCAGCAGGA GTCGCAGCCG CAGCAACAAC CGTAGCCGAA GCTACTCTCC ACGCCGCAGC 
CGAGGATCCC CGCAGTACTC GCCACGTCAT AGCCGTTCCC GCTCTCGCTC CTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_005157471.1
CDS139..912
Translation

Target ORF information:

RefSeq Version XM_005157414.4
Organism Danio rerio(zebrafish)
Definition Danio rerio serine/arginine-rich splicing factor 1a (srsf1a), transcript variant X1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 XM_005157414.4

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
ATGTCCGGTG GTGTGATCCG CGGCCCTGCT GGAAGCAACG ATTGCCGCAT CTATGTGGGA 
AACCTGCCGC CCGATATTCG CACCAAAGAT GTCGAAGATG TGTTTTATAA GTACGGAGCC 
ATCCGAGACA TCGATCTGAA AAACCGACGA GGCGGACCTC CATTCGCCTT TGTCGAGTTT 
GAAGACCCCA GAGATGCAGA GGATGCTGTT TATGCTCGTG ATGGCTATGA CTATGATGGC 
TATCGACTTC GAGTGGAATT TCCTAGGAGT GGACGAGGAA TGGGCAGAGG AGGTTTTGGT 
GGGGGTGGTG GAGGCGGTGG CGGCGGAGGA GGAGGTGGTG GTGGGGCTCC TAGAGGTAGA 
TATGGACCCC CATCCAGACG TTCAGAGTAC AGAGTAATTG TTTCAGGACT TCCACCAAGC 
GGCAGCTGGC AGGATCTGAA GGATCACATG CGTGAAGCAG GTGATGTATG TTACGCTGAC 
GTTTTCCGAG ATGGAACTGG TGTGGTGGAG TTTGTTCGCA AAGAGGACAT GACCTACGCC 
GTTCGCAAAC TGGATAACAC AAAATTCCGG TCCCATGAGG GAGAAACTGC GTACATCCGC 
GTAAAGGTGG ACGGACCCCG CAGTCCAAGC TATGGAAGAT CAAGGTCAAG GAGCCGCAGT 
CGCAGCAGGA GTCGCAGCCG CAGCAACAAC CGTAGCCGAA GCTACTCTCC ACGCCGCAGC 
CGAGGATCCC CGCAGTACTC GCCACGTCAT AGCCGTTCCC GCTCTCGCTC CTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

PubMed

book

Number of inadvertent RNA targets for morpholino knockdown in Danio rerio is largely underestimated: evidence from the study of Ser/Arg-rich splicing factors.
Nucleic acids research45(16)9547-9557(2017 Sep)
Joris M,Schloesser M,Baurain D,Hanikenne M,Muller M,Motte P


book

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
BMC genomics17368(2016 05)
Pasquier J,Cabau C,Nguyen T,Jouanno E,Severac D,Braasch I,Journot L,Pontarotti P,Klopp C,Postlethwait JH,Guiguen Y,Bobe J


book

Genome wide analysis reveals Zic3 interaction with distal regulatory elements of stage specific developmental genes in zebrafish.
PLoS genetics9(10)e1003852(2013 Oct)
Winata CL,Kondrychyn I,Kumar V,Srinivasan KG,Orlov Y,Ravishankar A,Prabhakar S,Stanton LW,Korzh V,Mathavan S