ORF cDNA Clones and Custom Clones » Species Summary » Danio rerio » harbi1 cDNA ORF clone

harbi1 cDNA ORF clone, Danio rerio(zebrafish)

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Summary

Gene Symbol	harbi1
Entrez Gene ID	445279
Full Name	harbinger transposase derived 1
Synonyms	flj32675,zgc:91866
General protein information	Preferred Names harbinger transposase derived 1 Names putative nuclease HARBI1 harbinger transposase-derived nuclease
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

Reference Sequences (RefSeq)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001003734.1	NP_001003734.1	putative nuclease HARBI1

Homologies

Pan troglodytes (chimpanzee)	HARBI1	XP_521904.2
	HARBI1	XP_001111962.1
Gallus gallus (chicken)	HARBI1	XP_421117.1
Arabidopsis thaliana (thale cress)	AT3G55350	NP_191095.1
Rattus norvegicus (Norway rat)	Harbi1	NP_001107265.2
Oryza sativa (rice)	Os01g0838900	NP_001044749.1
Canis lupus familiaris (dog)	HARBI1	XP_005631343.1
Bos taurus (cattle)	HARBI1	NP_001069136.1
Xenopus tropicalis (tropical clawed frog)	LOC100493758	XP_004913441.1
Homo sapiens (human)	HARBI1	NP_776172.1
Mus musculus (house mouse)	Harbi1	NP_848839.2
Danio rerio (zebrafish)	harbi1	NP_001003734.1
Oryza sativa (rice)	Os11g0702700	NP_001068533.1

RFX transcription factors are essential for hearing in mice.
Elkon R, Milon B, Morrison L, Shah M, Vijayakumar S, Racherla M, Leitch CC, Silipino L, Hadi S, Weiss-Gayet M, Barras E, Schmid CD, Ait-Lounis A, Barnes A, Song Y, Eisenman DJ, Eliyahu E, Frolenkov GI, Strome SE, Durand B, Zaghloul NA, Jones SM, Reith W, Hertzano R
Nature communications68549(2015 Oct)

Harbinger transposons and an ancient HARBI1 gene derived from a transposase.
Kapitonov VV, Jurka J
DNA and cell biology23(5)311-24(2004 May)

Runx1 is required for zebrafish blood and vessel development and expression of a human RUNX1-CBF2T1 transgene advances a model for studies of leukemogenesis.
Kalev-Zylinska ML, Horsfield JA, Flores MV, Postlethwait JH, Vitas MR, Baas AM, Crosier PS, Crosier KE
Development (Cambridge, England)129(8)2015-30(2002 Apr)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

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The following harbi1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the harbi1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

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***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ODa38112	NM_001003734.1 Latest version!	Danio rerio harbinger transposase derived 1 (harbi1), mRNA.	pcDNA3.1⁺/C-(K)DYK or customized vector	7-9	$258.30 $369.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

harbi1 ( NM_001003734.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_001003734.1 Danio rerio harbinger transposase derived 1 (harbi1), mRNA.

Price & Availability↑

CloneID	ODa38112
Clone ID Related Accession (Same CDS sequence)	NM_001003734.1
Accession Version	NM_001003734.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1050bp) Protein sequence SNP
Vector	pcDNA3.1⁺/C-(K)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-11-23
Organism	Danio rerio(zebrafish)
Product	putative nuclease HARBI1
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC078390.1. ##Evidence-Data-START## Transcript exon combination :: BC078390.1, BC100116.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2168446, SAMEA2168447 [ECO:0000348] ##Evidence-Data-END##

ATGGCACTCT CTATTGCAAT TCTAGACTGT GACCTGCTTC TGCATGGTCG TGGTCACAAA 
ACTCTTGATC GATTTGATAT CGAGACCGTG TCGGATGACT TTCTGCTGAA CACATTTGGC 
TTCCCACGTG AGTTCATTTA TTACCTGGTG GAACTGCTAA AGGATAGCTT GCTGAGACGC 
ACACAGCGGT CGCGAGCAAT TAGCCCAGAT GTTCAGATTC TTGCAGCCCT GGGATTCTAT 
ACATCCGGCT CTTTCCAGAG TAAGATGGGA GACGCGATTG GCATCAGCCA GGCTTCCATG 
AGTCGCTGCG TTTCTAACGT CACCAAAGCT CTGATCGAGA AAGCACCAGA GTTCATCGGC 
TTTACTAGGG ATGAGGCCAC CAAGCAGCAG TTTAAAGATG AGTTTTACCG AATTGCAGGA 
ATACCTAATG TGACGGGAGT TGTGGACTGT GCACATATAG CCATTAAAGC TCCCAATGCG 
GATGACTCTT CCTATGTAAA TAAAAAGGGA TTTCACTCGA TCAATTGCCA GTTAGTATGT 
GATGCCAGAG GACTGCTGTT GAGTGCTGAG ACACACTGGC CTGGTAGTTT GACAGACAGA 
GCGGTCTTTA AGCAGTCCAA TGTGGCAAAG CTGTTTGAAG AGCAAGAGAA TGATGATGAA 
GGTTGGCTTT TAGGTGACAA CCGATATCCT CTAAAGAAGT GGCTGATGAC TCCTGTGCAG 
AGTCCAGAGT CTCCTGCTGA CTACCGCTAT AACTTGGCTC ACACAACAAC GCATGAGATT 
GTAGACCGCA CATTTAGAGC CATTCAGACA CGTTTTCGGT GTTTGGATGG GGCGAAGGGT 
TACCTGCAGT ATTCCCCAGA GAAATGCTCT CATATAATTC AGGCCTGTTG TGTGCTGCAT 
AACATTTCCC TGCAGTCCGG CCTTGATGCC TGGACCTTTG AGAGGACAGA GGCCACCGAC 
CAATCAGGGG AAGATATCGA TCCATCTGAC ACTGATGACC CAGAGGCTCT GAGAGTTCGA 
CAGGAACTCA TCCAGAATCA TTTCAGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001003734.1
CDS	46..1095
Translation	MALSIAILDCDLLLHGRGHKTLDRFDIETVSDDFLLNTFGFPREFIYYLVELLKDSLLRRTQRSRAISPDVQILAALGFYTSGSFQSKMGDAIGISQASMSRCVSNVTKALIEKAPEFIGFTRDEATKQQFKDEFYRIAGIPNVTGVVDCAHIAIKAPNADDSSYVNKKGFHSINCQLVCDARGLLLSAETHWPGSLTDRAVFKQSNVAKLFEEQENDDEGWLLGDNRYPLKKWLMTPVQSPESPADYRYNLAHTTTHEIVDRTFRAIQTRFRCLDGAKGYLQYSPEKCSHIIQACCVLHNISLQSGLDAWTFERTEATDQSGEDIDPSDTDDPEALRVRQELIQNHFS

Target ORF information:

RefSeq Version	NM_001003734.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio harbinger transposase derived 1 (harbi1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001003734.1

ORF Insert Sequence:

ATGGCACTCT CTATTGCAAT TCTAGACTGT GACCTGCTTC TGCATGGTCG TGGTCACAAA 
ACTCTTGATC GATTTGATAT CGAGACCGTG TCGGATGACT TTCTGCTGAA CACATTTGGC 
TTCCCACGTG AGTTCATTTA TTACCTGGTG GAACTGCTAA AGGATAGCTT GCTGAGACGC 
ACACAGCGGT CGCGAGCAAT TAGCCCAGAT GTTCAGATTC TTGCAGCCCT GGGATTCTAT 
ACATCCGGCT CTTTCCAGAG TAAGATGGGA GACGCGATTG GCATCAGCCA GGCTTCCATG 
AGTCGCTGCG TTTCTAACGT CACCAAAGCT CTGATCGAGA AAGCACCAGA GTTCATCGGC 
TTTACTAGGG ATGAGGCCAC CAAGCAGCAG TTTAAAGATG AGTTTTACCG AATTGCAGGA 
ATACCTAATG TGACGGGAGT TGTGGACTGT GCACATATAG CCATTAAAGC TCCCAATGCG 
GATGACTCTT CCTATGTAAA TAAAAAGGGA TTTCACTCGA TCAATTGCCA GTTAGTATGT 
GATGCCAGAG GACTGCTGTT GAGTGCTGAG ACACACTGGC CTGGTAGTTT GACAGACAGA 
GCGGTCTTTA AGCAGTCCAA TGTGGCAAAG CTGTTTGAAG AGCAAGAGAA TGATGATGAA 
GGTTGGCTTT TAGGTGACAA CCGATATCCT CTAAAGAAGT GGCTGATGAC TCCTGTGCAG 
AGTCCAGAGT CTCCTGCTGA CTACCGCTAT AACTTGGCTC ACACAACAAC GCATGAGATT 
GTAGACCGCA CATTTAGAGC CATTCAGACA CGTTTTCGGT GTTTGGATGG GGCGAAGGGT 
TACCTGCAGT ATTCCCCAGA GAAATGCTCT CATATAATTC AGGCCTGTTG TGTGCTGCAT 
AACATTTCCC TGCAGTCCGG CCTTGATGCC TGGACCTTTG AGAGGACAGA GGCCACCGAC 
CAATCAGGGG AAGATATCGA TCCATCTGAC ACTGATGACC CAGAGGCTCT GAGAGTTCGA 
CAGGAACTCA TCCAGAATCA TTTCAGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

RFX transcription factors are essential for hearing in mice.
Nature communications68549(2015 Oct)
Elkon R,Milon B,Morrison L,Shah M,Vijayakumar S,Racherla M,Leitch CC,Silipino L,Hadi S,Weiss-Gayet M,Barras E,Schmid CD,Ait-Lounis A,Barnes A,Song Y,Eisenman DJ,Eliyahu E,Frolenkov GI,Strome SE,Durand B,Zaghloul NA,Jones SM,Reith W,Hertzano R

Harbinger transposons and an ancient HARBI1 gene derived from a transposase.
DNA and cell biology23(5)311-24(2004 May)
Kapitonov VV,Jurka J

Runx1 is required for zebrafish blood and vessel development and expression of a human RUNX1-CBF2T1 transgene advances a model for studies of leukemogenesis.
Development (Cambridge, England)129(8)2015-30(2002 Apr)
Kalev-Zylinska ML,Horsfield JA,Flores MV,Postlethwait JH,Vitas MR,Baas AM,Crosier PS,Crosier KE

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harbi1 cDNA ORF clone, Danio rerio(zebrafish)

Summary

Reference Sequences (RefSeq)

Pathways from BioSystems

Homologies

Gene Ontology

Bibliography

Reference Sequences (Refseq)

Target ORF information:

Target ORF information:

ORF Insert Sequence:

PubMed

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