lamb2l cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	lamb2l
Entrez Gene ID	553337
Full Name	laminin, beta 2-like
Synonyms	fc83a09,si:dkey-32e6.1,wu:fc83a09
General protein information	Preferred Names laminin, beta 2-like Names laminin subunit beta-1
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
XM_687746.9	XP_692838.6	laminin subunit beta-1 isoform X1
XM_005162121.4	XP_005162178.1	laminin subunit beta-1 isoform X1
XM_021473632.1	XP_021329307.1	laminin subunit beta-1 isoform X2
XM_005162121.3	XP_005162178.1	laminin subunit beta-1
XM_687746.8	XP_692838.6	laminin subunit beta-1
XM_021473631.1	XP_021329306.1	laminin subunit beta-1 isoform X2

Pathways from BioSystems

Homologies

Bos taurus (cattle)	LOC530599	XP_002697124.3
Danio rerio (zebrafish)	lamb2l	XP_005162178.1
Xenopus tropicalis (tropical clawed frog)	LOC100170549	NP_001123798.1

Gene Ontology

The following lamb2l gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the lamb2l cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODa146255	XM_021473632.1 Latest version!	Danio rerio laminin, beta 2-like (lamb2l), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
ODa146255	XM_021473631.1 Latest version!	Danio rerio laminin, beta 2-like (lamb2l), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
ODa27654	XM_005162121.4 Latest version!	Danio rerio laminin, beta 2-like (lamb2l), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
ODa27654	XM_687746.9 Latest version!	Danio rerio laminin, beta 2-like (lamb2l), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
View Old Accession Versions >>
ODa27654	XM_005162121.3	Danio rerio laminin, beta 2-like (lamb2l), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
ODa27654	XM_687746.8	Danio rerio laminin, beta 2-like (lamb2l), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

lamb2l ( XM_021473632.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021329307.1 Danio rerio laminin, beta 2-like (lamb2l), transcript variant X4, mRNA.

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CloneID	ODa146255
Clone ID Related Accession (Same CDS sequence)	XM_021473632.1 , XM_021473631.1
Accession Version	XM_021473632.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5511bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	laminin subunit beta-1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_018395143.1) and transcript sequence (GDQQ01003153.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## assembly gap :: added 1976 transcript bases to patch genome assembly gap ##RefSeq-Attributes-END##

ATGATGGAGA GATATTTCAG TGTTCTGCTT CTTGTTCTTC CTGCTTTGGC TCAGGATCTG 
CCCTCTGCCC CACATGGGTG CATGGATGGT AGCTGTTATC CAGCAACAGG CAACCTTCTG 
ATTGGCCGAG CTATAAACCT GACCACTTCC TCCACCTGTG GCCTGGATGG ACCCGAGCCA 
TACTGTATTG TCAGTCATTT GAAGGATTCA GAAAAGTGCT TTATATGTGA CTCTGAGAAT 
CCGTATGATG CCAATTATCA CAGAGACAGC CACAGAGTGG AGAACGTCAT CTACCCGATG 
GACAGCACAG GGGAACTCAC ATGGTGGCAG GCTGTAAATG GAGAGGAGAA TGTCAGTGTC 
AGACTGAATC TTGAGGCCGA GTTCCACTTT ACTCATCTCA TCATGAAGTT TAAGACATTT 
AGACCTGCTG CTATGATAAT TGAGCGTTCC TCTGATTTTG GCCGTACATG GAGGATATAC 
CGCTACTTTG CTTACAACTG CACTAAGACA TTTCCTAGGG TTCCTGCGCA CTCGCTGCGG 
TTCATTGATG AAGTCATTTG TGAAGAGCGC TACTCTGATA TTGAGCCATC CACTGATGGA 
GAGGTCATCT ACAAAGTGCT TGATCCTGCA ATCCATGTCA AAGATCCATA TAGTCTGGAC 
ATTCAGGATT TGTTGCGGAT CACTAACCTA CGGATAAACT TCACTAAACT GCACACTCTG 
GGTGATAACC TGCTTGACCG GCGTCCTGAC GTGCTGCAGA AGTATTACTA TGCATTGTAT 
GAGCTGGTTG TGCGCGGGAG CTGCTTCTGT TATGGCCATG CATCAGAGTG TGCACCAGTA 
CCTGGAGTGA CCACTAGAGA TTCAGGCATG ATTCATGGAC GCTGTGTCTG TAAGCACAAC 
ACTGTGGGAT TAAACTGTGA GCGCTGCAGA GATTTTTATC ATGATGCACC CTGGAGGCCT 
GCTGAGACCG ATAATGCACA CACCTGCAGG GAATGCAACT GTAATGGTCA CTCCAACCAG 
TGTCATTTTG ACATGGCGGT GTATTTGGCG ACGGGGAATG TCAGTGGAGG TGTGTGTAAC 
AACTGTCTTC ACAACACCAT GGGCCGCAAC TGTGAGAGCT GTAAGCCTTT CTACTACCAG 
GATCCCGAGA AAGACATCAG AGACCCGGCA GCGTGTGTCC CCTGTGACTG TGACCCCGTG 
GGCTCTGTGG AGGGAGGAGT GTGTGACAGT CATACAGATC TGGATTTGGG AATGATTGGT 
GGTCAGTGCC GTTGTAAGCA GAATGTCAGA GGTCAGCGCT GCGACTACTG TAAAGAGGGA 
CATTATGGTC TCAGTCTTGA TGACCCATTA GGATGCCAGC CTTGTAACTG TGACCCTCGT 
GGCATCACTA CACTGGGGGT TCCGTGTGAT CAGATTAGTG GGGACTGCTC TTGCAAAAGA 
TATGTAACAG GACGCTACTG CAACCAGTGC CTGCCTGAAT ATTGGGGACT CAGTCATGAT 
CTGGCTGGTT GCAGGCCCTG CAACTGTGAC TTTGGTGGAG CAACAAGCAG CAGATGCATG 
ATGGACAGCG GTCAGTGTGA CTGCCGGCCT CATCTGATTG GACGACAGTG TTCTGAAGTG 
CAGCCTGCAT TTTTCTGTGC TTCTTTGGAT TATTACAAGT ATGAAGCAGA AGCAGTTATG 
GGCCGCTCTC CTGAAGATCT CACACTTCCA GGCAGCCCAA GGCCTCAGGC TGAGACTGAC 
TGTGTGCAGC ATCTGAACAA TCAACTGAGA AGACACCGCC GTCACAGACA TATTGCACAA 
CAGCAGAGGG CAGCACTGAG ACGCATCCGA CAGCTGCAGC AAACACCCAG CGTGAGCTCA 
GTGCACAGAG AGAGACAGCC GGGTCAGATG GTCACCTGGA CCGGGCCGGG CTTCGCTCGG 
GTTAAAGACG GGGCTGGTCT TGAGTTTGCC ATCAGCAACA TCCCTTATGC CATGGAGTAT 
GACATTATGA TCCGATATGA GCCAGAGTCC ACAGAAGACT GGGAGGCTTT AGTGAGCGTC 
ACATCCGTGA ATTTACCCAT AAGCCTCCGC TGTGGCAACA TGCTGCCCAC AGAGCAGCTG 
TATACAGTCC CAATGCCCCA CAACGTGAGG TACATTAAAA TGCCAAAGCC CTTCTGCTTC 
GAGCCAAACA ACCGCTATGT GGTGTCCATT CGGTTCCAGC GCCACGCCGT GTCTCACCGC 
CATCTTACCG CCTTCATCCT GATAGACTCA CTTGTGCTGA TTCCCAGATA TGAAGAGCTT 
ACTGGATTTC AGGGTGATGA AGGTGCAGAT GAGCGTCGTC GTCAGGAGAT GGAGTCGTAC 
ATGTGTTTGG AGTCCTTCAT GACTATGCCA ATGCCAGCGC TGGCTGAACT GTGCACTTCT 
CTTATCTGCA GCATCTCTGC TATCATGCAC AATGGTGCAC TGGGGTGTCA GTGTGACCCT 
CAGGGTTCAT TAAGTGCAGA GTGTGACAGA GTCGGAGGTC AGTGTCGCTG TAAGCCCAAC 
GTGATTGGCC GTAAATGTAA TCAGTGTGCA CCGGGGACAT ATGGATTCGG CCCCTACGGC 
TGCACTGTAT GTGACTGTCA CTCGCAGGGT TCTGTCGGGC ATCATTGTGA CCCTGTGACG 
GGACAGTGTA TGTGTCGGCA TGGGGCGATT GGCCGCCAGT GCTCAGAATG CCAGCCGGGT 
CAGTGGGGAT TTCCTAACTG TAGGCAATGC CAGTGTAACA GCCATGCAGA GAGCTGCGAT 
CCTGAAAGCG GGGCCTGCTA TGACTGCAGA GACAACACAG CTGGACAACT GTGCGAGAGG 
TGTGTGGATG GTTATTATGG CAACCCAGCA CTTGGTTCTG GTGAGCACTG TCGCCCATGT 
CCGTGTCCAG GACACCCTGA CAGTGGACAT TCCAACGGTG CCTCCTGTGA AGTAGACTAC 
GCCTCCAACC AGATCCTTTG CCAGTGTGGG CAAGGCTACG CTGGTCCTCG CTGTGATCGC 
TGCGCCCCCG GTTATTATGG TGACCCCGAG CACCCTGGTG GATCGTGCCG GCCCTGCCAG 
TGCAATGGGA ACATTGACCC TCAGGACCCA GAGTCATGTG ACCCTCGAAC TGGACTGTGC 
CTCAAGTGTC TGTATAACAC AGATGGAGAC TCTTGCTCAG AGTGTAAACT GGGTTATTAT 
GGAAATGCAC TCATTCAGGA CTGTAGGCGA TGTACTTGTG TAACTGCAGG CACTTTGCCA 
AACTCTTGCA CTGATGGAGT GTGTCAATGT GATAAACAGA CAGGACAGTG CCCCTGCAAG 
CAAAACATCA TTGGACATAA CTGTGACCAG TGCGCCACCA ATCACTGGAA CTTCGGCTCT 
GACTGTGGAT GTGAACCCTG TGGATGTGTC CAACCTAATG CCCTCAGCAC CCACTGTAAC 
ATGTTTAGTG GCCAGTGTCA CTGTCAGCCA GGTTTTGGGG GGCGGCAGTG CAATGAGTGT 
GAGGCGTTCC ACTGGGGCGA TCCCAGAGTG CAATGTGAAG AGTGTAACTG CCATCCTCTG 
GGCTCTGAGA CGTCTCAGTG TGACCGCACA ACAGGCGCAT GTGTGTGTAA GGACGGAGCT 
TCAGGACAGC GCTGTGATGA GTGTGCACGC GGCTTTACTG GAAACTTCCC CAAATGTGTT 
CCCTGCCACC CCTGCTTCCA GCTGTGGGAT GATGTGGTGT GCCAGGTCCG CCGAGATCTG 
ACCCACTTCA GGGATATCAT TGCTATGATT CTGGAGAAGG GTGAGGTGCC TGGAGTCAGC 
GACAAGCGCA TACTTGAACT AGAGAAGAAG CTGGCACAGG CTCAAGAACT GGTCCGGGAT 
GGAGACAGAG AGGGAACATT TAACTTAATC AGCCAAACTG TTGATGACTT GAGAGCGGAG 
ATCGCTTTGA CTGATGGACG TCTGATGGGT GTAATACGGG ATCTGAATGT GACAACAGAC 
CATAACAATG ATCTGAAACA AAACCTAACT GCCCTGGAGA AAGAACTGAA AGATCTGAAC 
AATACGCTTC ACCAGCTGCG CAGAGAGCTG GAGAACTACC TCACTGCTGG ACTGGCAGAG 
CAATTTGCAA ATGTGCTGAA GTATTACCAG AGATCCCTGA ATTCAGAGCA GCGCTGCAAT 
GCCTCCGTAT ACGGACCCCA GAGTCCTGTG GAACAGTCTC AGGACACACG TAACCGTACC 
GAAGCCCTGC TGAAGGACAA GAAGGACAGC CTCCTGCGTA CAGCCACAGC TAACAATAAA 
TCCCTGTCTG AACTGGAGGG AAAAGCTCAT GAGATCAACC GAAAGGTCCA TCACCTTAGC 
AACAAGGTCT GCGGTGGCCA CGCCAATGGC AATGTGAATG GTAGCTGTGA AGAGAGCCCC 
TGTGGAGGAG CGGGATGTCG TGGCAGTGAT GGTGTGCTGA AGTGTGGAGG TAGAGGATGT 
AATGGAACTG TTGGTGCTTC AGTGAAGGCT TTAGACAATG CTGATGAGGT CCATAAGAAC 
CTGACTGCTG TTAGCGAGGA GCTCCAAACC ATGGCTAAGA AGCTACGAGA CATTGCTACG 
CTCACTCAAA CTGTGAAAAC ACAAGCCAAA GAAACTCTCG ATAAAGCCCA GAACAAGAAG 
GACATGTTTG AGAAGTCCAA CAAGATGCTC AAAAACTTCA TCCAGAAGAT TAAAGACTTT 
CTGAATGAGG AGGGAGCAGA TCCAGAAAGC ATTGAAAAAG TGGCCCAGCA GGTTCTGGCA 
ATCTCCTTGC CAGTCAACAG GACTGTAATA GTCAACTTAG TGGAGCAAAT CAAAGACAGC 
ATCGTCAACC TCACCAACGT GGAGGGAGTG TTCAATCACA CCTCAGAACA ACTGGCCAAA 
GTCAACGATC TACTCAAAAA AGCTCAGGAT GCAAAGACAC AAGCTGATGG AGTGAGTGAT 
AATATCAATA AAACAAAAGA AGCACTTGAA ACCTCACAGA ATGCCATAAA GAAAGCTGAA 
GAGGAAATGA GCACAGCTCT AAAAAACCTC AAGAATATCC AGAACATCAC TGCCATGGTT 
GACGACAAAC TCTTAAATTT AGACAACAAC CTTACAGATG TGATGATGCG TTTGGTCAAT 
TTGTCAAATG GAGTGGATTT ATTAAAGAAT AAAACGGAGC AGAACAGGGA GATGGCCAAA 
GAAGCCAAAG CCCAGTCGGA CAATGCAACT CGAGAAGCAG AAGGACTTCA AGAGGAGCTG 
ACAAATGCTG AGACTCTGTA CAAACAGCTG AAAGAGAAGG TGGACTCGGC TGGAGGGACA 
GGAGAAGGCA ATGTTAACCA GAAAGCCATA GAGATGAAGA AAGAAGCAGA AGATCTGCTG 
AAGAAGGCCA CCATGGGCAT GGAAACTCTC AAAAAATTGG AGAGAAAGTT CAACAAGAAC 
GAGCAGAAAA TGCAGCAGCA ACGGGATGAA CTGACAGATC TAGAGAAAAA TGTTACAGGC 
ATCAGAGAAT ATATCAGATC AAAAGTATTG GCCTACAACA ACTGTCAGTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021329307.1
CDS	311..5821
Translation	MMERYFSVLLLVLPALAQDLPSAPHGCMDGSCYPATGNLLIGRAINLTTSSTCGLDGPEPYCIVSHLKDSEKCFICDSENPYDANYHRDSHRVENVIYPMDSTGELTWWQAVNGEENVSVRLNLEAEFHFTHLIMKFKTFRPAAMIIERSSDFGRTWRIYRYFAYNCTKTFPRVPAHSLRFIDEVICEERYSDIEPSTDGEVIYKVLDPAIHVKDPYSLDIQDLLRITNLRINFTKLHTLGDNLLDRRPDVLQKYYYALYELVVRGSCFCYGHASECAPVPGVTTRDSGMIHGRCVCKHNTVGLNCERCRDFYHDAPWRPAETDNAHTCRECNCNGHSNQCHFDMAVYLATGNVSGGVCNNCLHNTMGRNCESCKPFYYQDPEKDIRDPAACVPCDCDPVGSVEGGVCDSHTDLDLGMIGGQCRCKQNVRGQRCDYCKEGHYGLSLDDPLGCQPCNCDPRGITTLGVPCDQISGDCSCKRYVTGRYCNQCLPEYWGLSHDLAGCRPCNCDFGGATSSRCMMDSGQCDCRPHLIGRQCSEVQPAFFCASLDYYKYEAEAVMGRSPEDLTLPGSPRPQAETDCVQHLNNQLRRHRRHRHIAQQQRAALRRIRQLQQTPSVSSVHRERQPGQMVTWTGPGFARVKDGAGLEFAISNIPYAMEYDIMIRYEPESTEDWEALVSVTSVNLPISLRCGNMLPTEQLYTVPMPHNVRYIKMPKPFCFEPNNRYVVSIRFQRHAVSHRHLTAFILIDSLVLIPRYEELTGFQGDEGADERRRQEMESYMCLESFMTMPMPALAELCTSLICSISAIMHNGALGCQCDPQGSLSAECDRVGGQCRCKPNVIGRKCNQCAPGTYGFGPYGCTVCDCHSQGSVGHHCDPVTGQCMCRHGAIGRQCSECQPGQWGFPNCRQCQCNSHAESCDPESGACYDCRDNTAGQLCERCVDGYYGNPALGSGEHCRPCPCPGHPDSGHSNGASCEVDYASNQILCQCGQGYAGPRCDRCAPGYYGDPEHPGGSCRPCQCNGNIDPQDPESCDPRTGLCLKCLYNTDGDSCSECKLGYYGNALIQDCRRCTCVTAGTLPNSCTDGVCQCDKQTGQCPCKQNIIGHNCDQCATNHWNFGSDCGCEPCGCVQPNALSTHCNMFSGQCHCQPGFGGRQCNECEAFHWGDPRVQCEECNCHPLGSETSQCDRTTGACVCKDGASGQRCDECARGFTGNFPKCVPCHPCFQLWDDVVCQVRRDLTHFRDIIAMILEKGEVPGVSDKRILELEKKLAQAQELVRDGDREGTFNLISQTVDDLRAEIALTDGRLMGVIRDLNVTTDHNNDLKQNLTALEKELKDLNNTLHQLRRELENYLTAGLAEQFANVLKYYQRSLNSEQRCNASVYGPQSPVEQSQDTRNRTEALLKDKKDSLLRTATANNKSLSELEGKAHEINRKVHHLSNKVCGGHANGNVNGSCEESPCGGAGCRGSDGVLKCGGRGCNGTVGASVKALDNADEVHKNLTAVSEELQTMAKKLRDIATLTQTVKTQAKETLDKAQNKKDMFEKSNKMLKNFIQKIKDFLNEEGADPESIEKVAQQVLAISLPVNRTVIVNLVEQIKDSIVNLTNVEGVFNHTSEQLAKVNDLLKKAQDAKTQADGVSDNINKTKEALETSQNAIKKAEEEMSTALKNLKNIQNITAMVDDKLLNLDNNLTDVMMRLVNLSNGVDLLKNKTEQNREMAKEAKAQSDNATREAEGLQEELTNAETLYKQLKEKVDSAGGTGEGNVNQKAIEMKKEAEDLLKKATMGMETLKKLERKFNKNEQKMQQQRDELTDLEKNVTGIREYIRSKVLAYNNCQ

Target ORF information:

RefSeq Version	XM_021473632.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio laminin, beta 2-like (lamb2l), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021473632.1

ORF Insert Sequence:

ATGATGGAGA GATATTTCAG TGTTCTGCTT CTTGTTCTTC CTGCTTTGGC TCAGGATCTG 
CCCTCTGCCC CACATGGGTG CATGGATGGT AGCTGTTATC CAGCAACAGG CAACCTTCTG 
ATTGGCCGAG CTATAAACCT GACCACTTCC TCCACCTGTG GCCTGGATGG ACCCGAGCCA 
TACTGTATTG TCAGTCATTT GAAGGATTCA GAAAAGTGCT TTATATGTGA CTCTGAGAAT 
CCGTATGATG CCAATTATCA CAGAGACAGC CACAGAGTGG AGAACGTCAT CTACCCGATG 
GACAGCACAG GGGAACTCAC ATGGTGGCAG GCTGTAAATG GAGAGGAGAA TGTCAGTGTC 
AGACTGAATC TTGAGGCCGA GTTCCACTTT ACTCATCTCA TCATGAAGTT TAAGACATTT 
AGACCTGCTG CTATGATAAT TGAGCGTTCC TCTGATTTTG GCCGTACATG GAGGATATAC 
CGCTACTTTG CTTACAACTG CACTAAGACA TTTCCTAGGG TTCCTGCGCA CTCGCTGCGG 
TTCATTGATG AAGTCATTTG TGAAGAGCGC TACTCTGATA TTGAGCCATC CACTGATGGA 
GAGGTCATCT ACAAAGTGCT TGATCCTGCA ATCCATGTCA AAGATCCATA TAGTCTGGAC 
ATTCAGGATT TGTTGCGGAT CACTAACCTA CGGATAAACT TCACTAAACT GCACACTCTG 
GGTGATAACC TGCTTGACCG GCGTCCTGAC GTGCTGCAGA AGTATTACTA TGCATTGTAT 
GAGCTGGTTG TGCGCGGGAG CTGCTTCTGT TATGGCCATG CATCAGAGTG TGCACCAGTA 
CCTGGAGTGA CCACTAGAGA TTCAGGCATG ATTCATGGAC GCTGTGTCTG TAAGCACAAC 
ACTGTGGGAT TAAACTGTGA GCGCTGCAGA GATTTTTATC ATGATGCACC CTGGAGGCCT 
GCTGAGACCG ATAATGCACA CACCTGCAGG GAATGCAACT GTAATGGTCA CTCCAACCAG 
TGTCATTTTG ACATGGCGGT GTATTTGGCG ACGGGGAATG TCAGTGGAGG TGTGTGTAAC 
AACTGTCTTC ACAACACCAT GGGCCGCAAC TGTGAGAGCT GTAAGCCTTT CTACTACCAG 
GATCCCGAGA AAGACATCAG AGACCCGGCA GCGTGTGTCC CCTGTGACTG TGACCCCGTG 
GGCTCTGTGG AGGGAGGAGT GTGTGACAGT CATACAGATC TGGATTTGGG AATGATTGGT 
GGTCAGTGCC GTTGTAAGCA GAATGTCAGA GGTCAGCGCT GCGACTACTG TAAAGAGGGA 
CATTATGGTC TCAGTCTTGA TGACCCATTA GGATGCCAGC CTTGTAACTG TGACCCTCGT 
GGCATCACTA CACTGGGGGT TCCGTGTGAT CAGATTAGTG GGGACTGCTC TTGCAAAAGA 
TATGTAACAG GACGCTACTG CAACCAGTGC CTGCCTGAAT ATTGGGGACT CAGTCATGAT 
CTGGCTGGTT GCAGGCCCTG CAACTGTGAC TTTGGTGGAG CAACAAGCAG CAGATGCATG 
ATGGACAGCG GTCAGTGTGA CTGCCGGCCT CATCTGATTG GACGACAGTG TTCTGAAGTG 
CAGCCTGCAT TTTTCTGTGC TTCTTTGGAT TATTACAAGT ATGAAGCAGA AGCAGTTATG 
GGCCGCTCTC CTGAAGATCT CACACTTCCA GGCAGCCCAA GGCCTCAGGC TGAGACTGAC 
TGTGTGCAGC ATCTGAACAA TCAACTGAGA AGACACCGCC GTCACAGACA TATTGCACAA 
CAGCAGAGGG CAGCACTGAG ACGCATCCGA CAGCTGCAGC AAACACCCAG CGTGAGCTCA 
GTGCACAGAG AGAGACAGCC GGGTCAGATG GTCACCTGGA CCGGGCCGGG CTTCGCTCGG 
GTTAAAGACG GGGCTGGTCT TGAGTTTGCC ATCAGCAACA TCCCTTATGC CATGGAGTAT 
GACATTATGA TCCGATATGA GCCAGAGTCC ACAGAAGACT GGGAGGCTTT AGTGAGCGTC 
ACATCCGTGA ATTTACCCAT AAGCCTCCGC TGTGGCAACA TGCTGCCCAC AGAGCAGCTG 
TATACAGTCC CAATGCCCCA CAACGTGAGG TACATTAAAA TGCCAAAGCC CTTCTGCTTC 
GAGCCAAACA ACCGCTATGT GGTGTCCATT CGGTTCCAGC GCCACGCCGT GTCTCACCGC 
CATCTTACCG CCTTCATCCT GATAGACTCA CTTGTGCTGA TTCCCAGATA TGAAGAGCTT 
ACTGGATTTC AGGGTGATGA AGGTGCAGAT GAGCGTCGTC GTCAGGAGAT GGAGTCGTAC 
ATGTGTTTGG AGTCCTTCAT GACTATGCCA ATGCCAGCGC TGGCTGAACT GTGCACTTCT 
CTTATCTGCA GCATCTCTGC TATCATGCAC AATGGTGCAC TGGGGTGTCA GTGTGACCCT 
CAGGGTTCAT TAAGTGCAGA GTGTGACAGA GTCGGAGGTC AGTGTCGCTG TAAGCCCAAC 
GTGATTGGCC GTAAATGTAA TCAGTGTGCA CCGGGGACAT ATGGATTCGG CCCCTACGGC 
TGCACTGTAT GTGACTGTCA CTCGCAGGGT TCTGTCGGGC ATCATTGTGA CCCTGTGACG 
GGACAGTGTA TGTGTCGGCA TGGGGCGATT GGCCGCCAGT GCTCAGAATG CCAGCCGGGT 
CAGTGGGGAT TTCCTAACTG TAGGCAATGC CAGTGTAACA GCCATGCAGA GAGCTGCGAT 
CCTGAAAGCG GGGCCTGCTA TGACTGCAGA GACAACACAG CTGGACAACT GTGCGAGAGG 
TGTGTGGATG GTTATTATGG CAACCCAGCA CTTGGTTCTG GTGAGCACTG TCGCCCATGT 
CCGTGTCCAG GACACCCTGA CAGTGGACAT TCCAACGGTG CCTCCTGTGA AGTAGACTAC 
GCCTCCAACC AGATCCTTTG CCAGTGTGGG CAAGGCTACG CTGGTCCTCG CTGTGATCGC 
TGCGCCCCCG GTTATTATGG TGACCCCGAG CACCCTGGTG GATCGTGCCG GCCCTGCCAG 
TGCAATGGGA ACATTGACCC TCAGGACCCA GAGTCATGTG ACCCTCGAAC TGGACTGTGC 
CTCAAGTGTC TGTATAACAC AGATGGAGAC TCTTGCTCAG AGTGTAAACT GGGTTATTAT 
GGAAATGCAC TCATTCAGGA CTGTAGGCGA TGTACTTGTG TAACTGCAGG CACTTTGCCA 
AACTCTTGCA CTGATGGAGT GTGTCAATGT GATAAACAGA CAGGACAGTG CCCCTGCAAG 
CAAAACATCA TTGGACATAA CTGTGACCAG TGCGCCACCA ATCACTGGAA CTTCGGCTCT 
GACTGTGGAT GTGAACCCTG TGGATGTGTC CAACCTAATG CCCTCAGCAC CCACTGTAAC 
ATGTTTAGTG GCCAGTGTCA CTGTCAGCCA GGTTTTGGGG GGCGGCAGTG CAATGAGTGT 
GAGGCGTTCC ACTGGGGCGA TCCCAGAGTG CAATGTGAAG AGTGTAACTG CCATCCTCTG 
GGCTCTGAGA CGTCTCAGTG TGACCGCACA ACAGGCGCAT GTGTGTGTAA GGACGGAGCT 
TCAGGACAGC GCTGTGATGA GTGTGCACGC GGCTTTACTG GAAACTTCCC CAAATGTGTT 
CCCTGCCACC CCTGCTTCCA GCTGTGGGAT GATGTGGTGT GCCAGGTCCG CCGAGATCTG 
ACCCACTTCA GGGATATCAT TGCTATGATT CTGGAGAAGG GTGAGGTGCC TGGAGTCAGC 
GACAAGCGCA TACTTGAACT AGAGAAGAAG CTGGCACAGG CTCAAGAACT GGTCCGGGAT 
GGAGACAGAG AGGGAACATT TAACTTAATC AGCCAAACTG TTGATGACTT GAGAGCGGAG 
ATCGCTTTGA CTGATGGACG TCTGATGGGT GTAATACGGG ATCTGAATGT GACAACAGAC 
CATAACAATG ATCTGAAACA AAACCTAACT GCCCTGGAGA AAGAACTGAA AGATCTGAAC 
AATACGCTTC ACCAGCTGCG CAGAGAGCTG GAGAACTACC TCACTGCTGG ACTGGCAGAG 
CAATTTGCAA ATGTGCTGAA GTATTACCAG AGATCCCTGA ATTCAGAGCA GCGCTGCAAT 
GCCTCCGTAT ACGGACCCCA GAGTCCTGTG GAACAGTCTC AGGACACACG TAACCGTACC 
GAAGCCCTGC TGAAGGACAA GAAGGACAGC CTCCTGCGTA CAGCCACAGC TAACAATAAA 
TCCCTGTCTG AACTGGAGGG AAAAGCTCAT GAGATCAACC GAAAGGTCCA TCACCTTAGC 
AACAAGGTCT GCGGTGGCCA CGCCAATGGC AATGTGAATG GTAGCTGTGA AGAGAGCCCC 
TGTGGAGGAG CGGGATGTCG TGGCAGTGAT GGTGTGCTGA AGTGTGGAGG TAGAGGATGT 
AATGGAACTG TTGGTGCTTC AGTGAAGGCT TTAGACAATG CTGATGAGGT CCATAAGAAC 
CTGACTGCTG TTAGCGAGGA GCTCCAAACC ATGGCTAAGA AGCTACGAGA CATTGCTACG 
CTCACTCAAA CTGTGAAAAC ACAAGCCAAA GAAACTCTCG ATAAAGCCCA GAACAAGAAG 
GACATGTTTG AGAAGTCCAA CAAGATGCTC AAAAACTTCA TCCAGAAGAT TAAAGACTTT 
CTGAATGAGG AGGGAGCAGA TCCAGAAAGC ATTGAAAAAG TGGCCCAGCA GGTTCTGGCA 
ATCTCCTTGC CAGTCAACAG GACTGTAATA GTCAACTTAG TGGAGCAAAT CAAAGACAGC 
ATCGTCAACC TCACCAACGT GGAGGGAGTG TTCAATCACA CCTCAGAACA ACTGGCCAAA 
GTCAACGATC TACTCAAAAA AGCTCAGGAT GCAAAGACAC AAGCTGATGG AGTGAGTGAT 
AATATCAATA AAACAAAAGA AGCACTTGAA ACCTCACAGA ATGCCATAAA GAAAGCTGAA 
GAGGAAATGA GCACAGCTCT AAAAAACCTC AAGAATATCC AGAACATCAC TGCCATGGTT 
GACGACAAAC TCTTAAATTT AGACAACAAC CTTACAGATG TGATGATGCG TTTGGTCAAT 
TTGTCAAATG GAGTGGATTT ATTAAAGAAT AAAACGGAGC AGAACAGGGA GATGGCCAAA 
GAAGCCAAAG CCCAGTCGGA CAATGCAACT CGAGAAGCAG AAGGACTTCA AGAGGAGCTG 
ACAAATGCTG AGACTCTGTA CAAACAGCTG AAAGAGAAGG TGGACTCGGC TGGAGGGACA 
GGAGAAGGCA ATGTTAACCA GAAAGCCATA GAGATGAAGA AAGAAGCAGA AGATCTGCTG 
AAGAAGGCCA CCATGGGCAT GGAAACTCTC AAAAAATTGG AGAGAAAGTT CAACAAGAAC 
GAGCAGAAAA TGCAGCAGCA ACGGGATGAA CTGACAGATC TAGAGAAAAA TGTTACAGGC 
ATCAGAGAAT ATATCAGATC AAAAGTATTG GCCTACAACA ACTGTCAGTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

lamb2l ( XM_021473631.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021329306.1 Danio rerio laminin, beta 2-like (lamb2l), transcript variant X3, mRNA.

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CloneID	ODa146255
Clone ID Related Accession (Same CDS sequence)	XM_021473632.1 , XM_021473631.1
Accession Version	XM_021473631.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5511bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	laminin subunit beta-1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_018395143.1) and transcript sequence (GDQQ01003153.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## assembly gap :: added 1976 transcript bases to patch genome assembly gap ##RefSeq-Attributes-END##

ATGATGGAGA GATATTTCAG TGTTCTGCTT CTTGTTCTTC CTGCTTTGGC TCAGGATCTG 
CCCTCTGCCC CACATGGGTG CATGGATGGT AGCTGTTATC CAGCAACAGG CAACCTTCTG 
ATTGGCCGAG CTATAAACCT GACCACTTCC TCCACCTGTG GCCTGGATGG ACCCGAGCCA 
TACTGTATTG TCAGTCATTT GAAGGATTCA GAAAAGTGCT TTATATGTGA CTCTGAGAAT 
CCGTATGATG CCAATTATCA CAGAGACAGC CACAGAGTGG AGAACGTCAT CTACCCGATG 
GACAGCACAG GGGAACTCAC ATGGTGGCAG GCTGTAAATG GAGAGGAGAA TGTCAGTGTC 
AGACTGAATC TTGAGGCCGA GTTCCACTTT ACTCATCTCA TCATGAAGTT TAAGACATTT 
AGACCTGCTG CTATGATAAT TGAGCGTTCC TCTGATTTTG GCCGTACATG GAGGATATAC 
CGCTACTTTG CTTACAACTG CACTAAGACA TTTCCTAGGG TTCCTGCGCA CTCGCTGCGG 
TTCATTGATG AAGTCATTTG TGAAGAGCGC TACTCTGATA TTGAGCCATC CACTGATGGA 
GAGGTCATCT ACAAAGTGCT TGATCCTGCA ATCCATGTCA AAGATCCATA TAGTCTGGAC 
ATTCAGGATT TGTTGCGGAT CACTAACCTA CGGATAAACT TCACTAAACT GCACACTCTG 
GGTGATAACC TGCTTGACCG GCGTCCTGAC GTGCTGCAGA AGTATTACTA TGCATTGTAT 
GAGCTGGTTG TGCGCGGGAG CTGCTTCTGT TATGGCCATG CATCAGAGTG TGCACCAGTA 
CCTGGAGTGA CCACTAGAGA TTCAGGCATG ATTCATGGAC GCTGTGTCTG TAAGCACAAC 
ACTGTGGGAT TAAACTGTGA GCGCTGCAGA GATTTTTATC ATGATGCACC CTGGAGGCCT 
GCTGAGACCG ATAATGCACA CACCTGCAGG GAATGCAACT GTAATGGTCA CTCCAACCAG 
TGTCATTTTG ACATGGCGGT GTATTTGGCG ACGGGGAATG TCAGTGGAGG TGTGTGTAAC 
AACTGTCTTC ACAACACCAT GGGCCGCAAC TGTGAGAGCT GTAAGCCTTT CTACTACCAG 
GATCCCGAGA AAGACATCAG AGACCCGGCA GCGTGTGTCC CCTGTGACTG TGACCCCGTG 
GGCTCTGTGG AGGGAGGAGT GTGTGACAGT CATACAGATC TGGATTTGGG AATGATTGGT 
GGTCAGTGCC GTTGTAAGCA GAATGTCAGA GGTCAGCGCT GCGACTACTG TAAAGAGGGA 
CATTATGGTC TCAGTCTTGA TGACCCATTA GGATGCCAGC CTTGTAACTG TGACCCTCGT 
GGCATCACTA CACTGGGGGT TCCGTGTGAT CAGATTAGTG GGGACTGCTC TTGCAAAAGA 
TATGTAACAG GACGCTACTG CAACCAGTGC CTGCCTGAAT ATTGGGGACT CAGTCATGAT 
CTGGCTGGTT GCAGGCCCTG CAACTGTGAC TTTGGTGGAG CAACAAGCAG CAGATGCATG 
ATGGACAGCG GTCAGTGTGA CTGCCGGCCT CATCTGATTG GACGACAGTG TTCTGAAGTG 
CAGCCTGCAT TTTTCTGTGC TTCTTTGGAT TATTACAAGT ATGAAGCAGA AGCAGTTATG 
GGCCGCTCTC CTGAAGATCT CACACTTCCA GGCAGCCCAA GGCCTCAGGC TGAGACTGAC 
TGTGTGCAGC ATCTGAACAA TCAACTGAGA AGACACCGCC GTCACAGACA TATTGCACAA 
CAGCAGAGGG CAGCACTGAG ACGCATCCGA CAGCTGCAGC AAACACCCAG CGTGAGCTCA 
GTGCACAGAG AGAGACAGCC GGGTCAGATG GTCACCTGGA CCGGGCCGGG CTTCGCTCGG 
GTTAAAGACG GGGCTGGTCT TGAGTTTGCC ATCAGCAACA TCCCTTATGC CATGGAGTAT 
GACATTATGA TCCGATATGA GCCAGAGTCC ACAGAAGACT GGGAGGCTTT AGTGAGCGTC 
ACATCCGTGA ATTTACCCAT AAGCCTCCGC TGTGGCAACA TGCTGCCCAC AGAGCAGCTG 
TATACAGTCC CAATGCCCCA CAACGTGAGG TACATTAAAA TGCCAAAGCC CTTCTGCTTC 
GAGCCAAACA ACCGCTATGT GGTGTCCATT CGGTTCCAGC GCCACGCCGT GTCTCACCGC 
CATCTTACCG CCTTCATCCT GATAGACTCA CTTGTGCTGA TTCCCAGATA TGAAGAGCTT 
ACTGGATTTC AGGGTGATGA AGGTGCAGAT GAGCGTCGTC GTCAGGAGAT GGAGTCGTAC 
ATGTGTTTGG AGTCCTTCAT GACTATGCCA ATGCCAGCGC TGGCTGAACT GTGCACTTCT 
CTTATCTGCA GCATCTCTGC TATCATGCAC AATGGTGCAC TGGGGTGTCA GTGTGACCCT 
CAGGGTTCAT TAAGTGCAGA GTGTGACAGA GTCGGAGGTC AGTGTCGCTG TAAGCCCAAC 
GTGATTGGCC GTAAATGTAA TCAGTGTGCA CCGGGGACAT ATGGATTCGG CCCCTACGGC 
TGCACTGTAT GTGACTGTCA CTCGCAGGGT TCTGTCGGGC ATCATTGTGA CCCTGTGACG 
GGACAGTGTA TGTGTCGGCA TGGGGCGATT GGCCGCCAGT GCTCAGAATG CCAGCCGGGT 
CAGTGGGGAT TTCCTAACTG TAGGCAATGC CAGTGTAACA GCCATGCAGA GAGCTGCGAT 
CCTGAAAGCG GGGCCTGCTA TGACTGCAGA GACAACACAG CTGGACAACT GTGCGAGAGG 
TGTGTGGATG GTTATTATGG CAACCCAGCA CTTGGTTCTG GTGAGCACTG TCGCCCATGT 
CCGTGTCCAG GACACCCTGA CAGTGGACAT TCCAACGGTG CCTCCTGTGA AGTAGACTAC 
GCCTCCAACC AGATCCTTTG CCAGTGTGGG CAAGGCTACG CTGGTCCTCG CTGTGATCGC 
TGCGCCCCCG GTTATTATGG TGACCCCGAG CACCCTGGTG GATCGTGCCG GCCCTGCCAG 
TGCAATGGGA ACATTGACCC TCAGGACCCA GAGTCATGTG ACCCTCGAAC TGGACTGTGC 
CTCAAGTGTC TGTATAACAC AGATGGAGAC TCTTGCTCAG AGTGTAAACT GGGTTATTAT 
GGAAATGCAC TCATTCAGGA CTGTAGGCGA TGTACTTGTG TAACTGCAGG CACTTTGCCA 
AACTCTTGCA CTGATGGAGT GTGTCAATGT GATAAACAGA CAGGACAGTG CCCCTGCAAG 
CAAAACATCA TTGGACATAA CTGTGACCAG TGCGCCACCA ATCACTGGAA CTTCGGCTCT 
GACTGTGGAT GTGAACCCTG TGGATGTGTC CAACCTAATG CCCTCAGCAC CCACTGTAAC 
ATGTTTAGTG GCCAGTGTCA CTGTCAGCCA GGTTTTGGGG GGCGGCAGTG CAATGAGTGT 
GAGGCGTTCC ACTGGGGCGA TCCCAGAGTG CAATGTGAAG AGTGTAACTG CCATCCTCTG 
GGCTCTGAGA CGTCTCAGTG TGACCGCACA ACAGGCGCAT GTGTGTGTAA GGACGGAGCT 
TCAGGACAGC GCTGTGATGA GTGTGCACGC GGCTTTACTG GAAACTTCCC CAAATGTGTT 
CCCTGCCACC CCTGCTTCCA GCTGTGGGAT GATGTGGTGT GCCAGGTCCG CCGAGATCTG 
ACCCACTTCA GGGATATCAT TGCTATGATT CTGGAGAAGG GTGAGGTGCC TGGAGTCAGC 
GACAAGCGCA TACTTGAACT AGAGAAGAAG CTGGCACAGG CTCAAGAACT GGTCCGGGAT 
GGAGACAGAG AGGGAACATT TAACTTAATC AGCCAAACTG TTGATGACTT GAGAGCGGAG 
ATCGCTTTGA CTGATGGACG TCTGATGGGT GTAATACGGG ATCTGAATGT GACAACAGAC 
CATAACAATG ATCTGAAACA AAACCTAACT GCCCTGGAGA AAGAACTGAA AGATCTGAAC 
AATACGCTTC ACCAGCTGCG CAGAGAGCTG GAGAACTACC TCACTGCTGG ACTGGCAGAG 
CAATTTGCAA ATGTGCTGAA GTATTACCAG AGATCCCTGA ATTCAGAGCA GCGCTGCAAT 
GCCTCCGTAT ACGGACCCCA GAGTCCTGTG GAACAGTCTC AGGACACACG TAACCGTACC 
GAAGCCCTGC TGAAGGACAA GAAGGACAGC CTCCTGCGTA CAGCCACAGC TAACAATAAA 
TCCCTGTCTG AACTGGAGGG AAAAGCTCAT GAGATCAACC GAAAGGTCCA TCACCTTAGC 
AACAAGGTCT GCGGTGGCCA CGCCAATGGC AATGTGAATG GTAGCTGTGA AGAGAGCCCC 
TGTGGAGGAG CGGGATGTCG TGGCAGTGAT GGTGTGCTGA AGTGTGGAGG TAGAGGATGT 
AATGGAACTG TTGGTGCTTC AGTGAAGGCT TTAGACAATG CTGATGAGGT CCATAAGAAC 
CTGACTGCTG TTAGCGAGGA GCTCCAAACC ATGGCTAAGA AGCTACGAGA CATTGCTACG 
CTCACTCAAA CTGTGAAAAC ACAAGCCAAA GAAACTCTCG ATAAAGCCCA GAACAAGAAG 
GACATGTTTG AGAAGTCCAA CAAGATGCTC AAAAACTTCA TCCAGAAGAT TAAAGACTTT 
CTGAATGAGG AGGGAGCAGA TCCAGAAAGC ATTGAAAAAG TGGCCCAGCA GGTTCTGGCA 
ATCTCCTTGC CAGTCAACAG GACTGTAATA GTCAACTTAG TGGAGCAAAT CAAAGACAGC 
ATCGTCAACC TCACCAACGT GGAGGGAGTG TTCAATCACA CCTCAGAACA ACTGGCCAAA 
GTCAACGATC TACTCAAAAA AGCTCAGGAT GCAAAGACAC AAGCTGATGG AGTGAGTGAT 
AATATCAATA AAACAAAAGA AGCACTTGAA ACCTCACAGA ATGCCATAAA GAAAGCTGAA 
GAGGAAATGA GCACAGCTCT AAAAAACCTC AAGAATATCC AGAACATCAC TGCCATGGTT 
GACGACAAAC TCTTAAATTT AGACAACAAC CTTACAGATG TGATGATGCG TTTGGTCAAT 
TTGTCAAATG GAGTGGATTT ATTAAAGAAT AAAACGGAGC AGAACAGGGA GATGGCCAAA 
GAAGCCAAAG CCCAGTCGGA CAATGCAACT CGAGAAGCAG AAGGACTTCA AGAGGAGCTG 
ACAAATGCTG AGACTCTGTA CAAACAGCTG AAAGAGAAGG TGGACTCGGC TGGAGGGACA 
GGAGAAGGCA ATGTTAACCA GAAAGCCATA GAGATGAAGA AAGAAGCAGA AGATCTGCTG 
AAGAAGGCCA CCATGGGCAT GGAAACTCTC AAAAAATTGG AGAGAAAGTT CAACAAGAAC 
GAGCAGAAAA TGCAGCAGCA ACGGGATGAA CTGACAGATC TAGAGAAAAA TGTTACAGGC 
ATCAGAGAAT ATATCAGATC AAAAGTATTG GCCTACAACA ACTGTCAGTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021329306.1
CDS	403..5913
Translation	MMERYFSVLLLVLPALAQDLPSAPHGCMDGSCYPATGNLLIGRAINLTTSSTCGLDGPEPYCIVSHLKDSEKCFICDSENPYDANYHRDSHRVENVIYPMDSTGELTWWQAVNGEENVSVRLNLEAEFHFTHLIMKFKTFRPAAMIIERSSDFGRTWRIYRYFAYNCTKTFPRVPAHSLRFIDEVICEERYSDIEPSTDGEVIYKVLDPAIHVKDPYSLDIQDLLRITNLRINFTKLHTLGDNLLDRRPDVLQKYYYALYELVVRGSCFCYGHASECAPVPGVTTRDSGMIHGRCVCKHNTVGLNCERCRDFYHDAPWRPAETDNAHTCRECNCNGHSNQCHFDMAVYLATGNVSGGVCNNCLHNTMGRNCESCKPFYYQDPEKDIRDPAACVPCDCDPVGSVEGGVCDSHTDLDLGMIGGQCRCKQNVRGQRCDYCKEGHYGLSLDDPLGCQPCNCDPRGITTLGVPCDQISGDCSCKRYVTGRYCNQCLPEYWGLSHDLAGCRPCNCDFGGATSSRCMMDSGQCDCRPHLIGRQCSEVQPAFFCASLDYYKYEAEAVMGRSPEDLTLPGSPRPQAETDCVQHLNNQLRRHRRHRHIAQQQRAALRRIRQLQQTPSVSSVHRERQPGQMVTWTGPGFARVKDGAGLEFAISNIPYAMEYDIMIRYEPESTEDWEALVSVTSVNLPISLRCGNMLPTEQLYTVPMPHNVRYIKMPKPFCFEPNNRYVVSIRFQRHAVSHRHLTAFILIDSLVLIPRYEELTGFQGDEGADERRRQEMESYMCLESFMTMPMPALAELCTSLICSISAIMHNGALGCQCDPQGSLSAECDRVGGQCRCKPNVIGRKCNQCAPGTYGFGPYGCTVCDCHSQGSVGHHCDPVTGQCMCRHGAIGRQCSECQPGQWGFPNCRQCQCNSHAESCDPESGACYDCRDNTAGQLCERCVDGYYGNPALGSGEHCRPCPCPGHPDSGHSNGASCEVDYASNQILCQCGQGYAGPRCDRCAPGYYGDPEHPGGSCRPCQCNGNIDPQDPESCDPRTGLCLKCLYNTDGDSCSECKLGYYGNALIQDCRRCTCVTAGTLPNSCTDGVCQCDKQTGQCPCKQNIIGHNCDQCATNHWNFGSDCGCEPCGCVQPNALSTHCNMFSGQCHCQPGFGGRQCNECEAFHWGDPRVQCEECNCHPLGSETSQCDRTTGACVCKDGASGQRCDECARGFTGNFPKCVPCHPCFQLWDDVVCQVRRDLTHFRDIIAMILEKGEVPGVSDKRILELEKKLAQAQELVRDGDREGTFNLISQTVDDLRAEIALTDGRLMGVIRDLNVTTDHNNDLKQNLTALEKELKDLNNTLHQLRRELENYLTAGLAEQFANVLKYYQRSLNSEQRCNASVYGPQSPVEQSQDTRNRTEALLKDKKDSLLRTATANNKSLSELEGKAHEINRKVHHLSNKVCGGHANGNVNGSCEESPCGGAGCRGSDGVLKCGGRGCNGTVGASVKALDNADEVHKNLTAVSEELQTMAKKLRDIATLTQTVKTQAKETLDKAQNKKDMFEKSNKMLKNFIQKIKDFLNEEGADPESIEKVAQQVLAISLPVNRTVIVNLVEQIKDSIVNLTNVEGVFNHTSEQLAKVNDLLKKAQDAKTQADGVSDNINKTKEALETSQNAIKKAEEEMSTALKNLKNIQNITAMVDDKLLNLDNNLTDVMMRLVNLSNGVDLLKNKTEQNREMAKEAKAQSDNATREAEGLQEELTNAETLYKQLKEKVDSAGGTGEGNVNQKAIEMKKEAEDLLKKATMGMETLKKLERKFNKNEQKMQQQRDELTDLEKNVTGIREYIRSKVLAYNNCQ

Target ORF information:

RefSeq Version	XM_021473631.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio laminin, beta 2-like (lamb2l), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021473631.1

ORF Insert Sequence:

ATGATGGAGA GATATTTCAG TGTTCTGCTT CTTGTTCTTC CTGCTTTGGC TCAGGATCTG 
CCCTCTGCCC CACATGGGTG CATGGATGGT AGCTGTTATC CAGCAACAGG CAACCTTCTG 
ATTGGCCGAG CTATAAACCT GACCACTTCC TCCACCTGTG GCCTGGATGG ACCCGAGCCA 
TACTGTATTG TCAGTCATTT GAAGGATTCA GAAAAGTGCT TTATATGTGA CTCTGAGAAT 
CCGTATGATG CCAATTATCA CAGAGACAGC CACAGAGTGG AGAACGTCAT CTACCCGATG 
GACAGCACAG GGGAACTCAC ATGGTGGCAG GCTGTAAATG GAGAGGAGAA TGTCAGTGTC 
AGACTGAATC TTGAGGCCGA GTTCCACTTT ACTCATCTCA TCATGAAGTT TAAGACATTT 
AGACCTGCTG CTATGATAAT TGAGCGTTCC TCTGATTTTG GCCGTACATG GAGGATATAC 
CGCTACTTTG CTTACAACTG CACTAAGACA TTTCCTAGGG TTCCTGCGCA CTCGCTGCGG 
TTCATTGATG AAGTCATTTG TGAAGAGCGC TACTCTGATA TTGAGCCATC CACTGATGGA 
GAGGTCATCT ACAAAGTGCT TGATCCTGCA ATCCATGTCA AAGATCCATA TAGTCTGGAC 
ATTCAGGATT TGTTGCGGAT CACTAACCTA CGGATAAACT TCACTAAACT GCACACTCTG 
GGTGATAACC TGCTTGACCG GCGTCCTGAC GTGCTGCAGA AGTATTACTA TGCATTGTAT 
GAGCTGGTTG TGCGCGGGAG CTGCTTCTGT TATGGCCATG CATCAGAGTG TGCACCAGTA 
CCTGGAGTGA CCACTAGAGA TTCAGGCATG ATTCATGGAC GCTGTGTCTG TAAGCACAAC 
ACTGTGGGAT TAAACTGTGA GCGCTGCAGA GATTTTTATC ATGATGCACC CTGGAGGCCT 
GCTGAGACCG ATAATGCACA CACCTGCAGG GAATGCAACT GTAATGGTCA CTCCAACCAG 
TGTCATTTTG ACATGGCGGT GTATTTGGCG ACGGGGAATG TCAGTGGAGG TGTGTGTAAC 
AACTGTCTTC ACAACACCAT GGGCCGCAAC TGTGAGAGCT GTAAGCCTTT CTACTACCAG 
GATCCCGAGA AAGACATCAG AGACCCGGCA GCGTGTGTCC CCTGTGACTG TGACCCCGTG 
GGCTCTGTGG AGGGAGGAGT GTGTGACAGT CATACAGATC TGGATTTGGG AATGATTGGT 
GGTCAGTGCC GTTGTAAGCA GAATGTCAGA GGTCAGCGCT GCGACTACTG TAAAGAGGGA 
CATTATGGTC TCAGTCTTGA TGACCCATTA GGATGCCAGC CTTGTAACTG TGACCCTCGT 
GGCATCACTA CACTGGGGGT TCCGTGTGAT CAGATTAGTG GGGACTGCTC TTGCAAAAGA 
TATGTAACAG GACGCTACTG CAACCAGTGC CTGCCTGAAT ATTGGGGACT CAGTCATGAT 
CTGGCTGGTT GCAGGCCCTG CAACTGTGAC TTTGGTGGAG CAACAAGCAG CAGATGCATG 
ATGGACAGCG GTCAGTGTGA CTGCCGGCCT CATCTGATTG GACGACAGTG TTCTGAAGTG 
CAGCCTGCAT TTTTCTGTGC TTCTTTGGAT TATTACAAGT ATGAAGCAGA AGCAGTTATG 
GGCCGCTCTC CTGAAGATCT CACACTTCCA GGCAGCCCAA GGCCTCAGGC TGAGACTGAC 
TGTGTGCAGC ATCTGAACAA TCAACTGAGA AGACACCGCC GTCACAGACA TATTGCACAA 
CAGCAGAGGG CAGCACTGAG ACGCATCCGA CAGCTGCAGC AAACACCCAG CGTGAGCTCA 
GTGCACAGAG AGAGACAGCC GGGTCAGATG GTCACCTGGA CCGGGCCGGG CTTCGCTCGG 
GTTAAAGACG GGGCTGGTCT TGAGTTTGCC ATCAGCAACA TCCCTTATGC CATGGAGTAT 
GACATTATGA TCCGATATGA GCCAGAGTCC ACAGAAGACT GGGAGGCTTT AGTGAGCGTC 
ACATCCGTGA ATTTACCCAT AAGCCTCCGC TGTGGCAACA TGCTGCCCAC AGAGCAGCTG 
TATACAGTCC CAATGCCCCA CAACGTGAGG TACATTAAAA TGCCAAAGCC CTTCTGCTTC 
GAGCCAAACA ACCGCTATGT GGTGTCCATT CGGTTCCAGC GCCACGCCGT GTCTCACCGC 
CATCTTACCG CCTTCATCCT GATAGACTCA CTTGTGCTGA TTCCCAGATA TGAAGAGCTT 
ACTGGATTTC AGGGTGATGA AGGTGCAGAT GAGCGTCGTC GTCAGGAGAT GGAGTCGTAC 
ATGTGTTTGG AGTCCTTCAT GACTATGCCA ATGCCAGCGC TGGCTGAACT GTGCACTTCT 
CTTATCTGCA GCATCTCTGC TATCATGCAC AATGGTGCAC TGGGGTGTCA GTGTGACCCT 
CAGGGTTCAT TAAGTGCAGA GTGTGACAGA GTCGGAGGTC AGTGTCGCTG TAAGCCCAAC 
GTGATTGGCC GTAAATGTAA TCAGTGTGCA CCGGGGACAT ATGGATTCGG CCCCTACGGC 
TGCACTGTAT GTGACTGTCA CTCGCAGGGT TCTGTCGGGC ATCATTGTGA CCCTGTGACG 
GGACAGTGTA TGTGTCGGCA TGGGGCGATT GGCCGCCAGT GCTCAGAATG CCAGCCGGGT 
CAGTGGGGAT TTCCTAACTG TAGGCAATGC CAGTGTAACA GCCATGCAGA GAGCTGCGAT 
CCTGAAAGCG GGGCCTGCTA TGACTGCAGA GACAACACAG CTGGACAACT GTGCGAGAGG 
TGTGTGGATG GTTATTATGG CAACCCAGCA CTTGGTTCTG GTGAGCACTG TCGCCCATGT 
CCGTGTCCAG GACACCCTGA CAGTGGACAT TCCAACGGTG CCTCCTGTGA AGTAGACTAC 
GCCTCCAACC AGATCCTTTG CCAGTGTGGG CAAGGCTACG CTGGTCCTCG CTGTGATCGC 
TGCGCCCCCG GTTATTATGG TGACCCCGAG CACCCTGGTG GATCGTGCCG GCCCTGCCAG 
TGCAATGGGA ACATTGACCC TCAGGACCCA GAGTCATGTG ACCCTCGAAC TGGACTGTGC 
CTCAAGTGTC TGTATAACAC AGATGGAGAC TCTTGCTCAG AGTGTAAACT GGGTTATTAT 
GGAAATGCAC TCATTCAGGA CTGTAGGCGA TGTACTTGTG TAACTGCAGG CACTTTGCCA 
AACTCTTGCA CTGATGGAGT GTGTCAATGT GATAAACAGA CAGGACAGTG CCCCTGCAAG 
CAAAACATCA TTGGACATAA CTGTGACCAG TGCGCCACCA ATCACTGGAA CTTCGGCTCT 
GACTGTGGAT GTGAACCCTG TGGATGTGTC CAACCTAATG CCCTCAGCAC CCACTGTAAC 
ATGTTTAGTG GCCAGTGTCA CTGTCAGCCA GGTTTTGGGG GGCGGCAGTG CAATGAGTGT 
GAGGCGTTCC ACTGGGGCGA TCCCAGAGTG CAATGTGAAG AGTGTAACTG CCATCCTCTG 
GGCTCTGAGA CGTCTCAGTG TGACCGCACA ACAGGCGCAT GTGTGTGTAA GGACGGAGCT 
TCAGGACAGC GCTGTGATGA GTGTGCACGC GGCTTTACTG GAAACTTCCC CAAATGTGTT 
CCCTGCCACC CCTGCTTCCA GCTGTGGGAT GATGTGGTGT GCCAGGTCCG CCGAGATCTG 
ACCCACTTCA GGGATATCAT TGCTATGATT CTGGAGAAGG GTGAGGTGCC TGGAGTCAGC 
GACAAGCGCA TACTTGAACT AGAGAAGAAG CTGGCACAGG CTCAAGAACT GGTCCGGGAT 
GGAGACAGAG AGGGAACATT TAACTTAATC AGCCAAACTG TTGATGACTT GAGAGCGGAG 
ATCGCTTTGA CTGATGGACG TCTGATGGGT GTAATACGGG ATCTGAATGT GACAACAGAC 
CATAACAATG ATCTGAAACA AAACCTAACT GCCCTGGAGA AAGAACTGAA AGATCTGAAC 
AATACGCTTC ACCAGCTGCG CAGAGAGCTG GAGAACTACC TCACTGCTGG ACTGGCAGAG 
CAATTTGCAA ATGTGCTGAA GTATTACCAG AGATCCCTGA ATTCAGAGCA GCGCTGCAAT 
GCCTCCGTAT ACGGACCCCA GAGTCCTGTG GAACAGTCTC AGGACACACG TAACCGTACC 
GAAGCCCTGC TGAAGGACAA GAAGGACAGC CTCCTGCGTA CAGCCACAGC TAACAATAAA 
TCCCTGTCTG AACTGGAGGG AAAAGCTCAT GAGATCAACC GAAAGGTCCA TCACCTTAGC 
AACAAGGTCT GCGGTGGCCA CGCCAATGGC AATGTGAATG GTAGCTGTGA AGAGAGCCCC 
TGTGGAGGAG CGGGATGTCG TGGCAGTGAT GGTGTGCTGA AGTGTGGAGG TAGAGGATGT 
AATGGAACTG TTGGTGCTTC AGTGAAGGCT TTAGACAATG CTGATGAGGT CCATAAGAAC 
CTGACTGCTG TTAGCGAGGA GCTCCAAACC ATGGCTAAGA AGCTACGAGA CATTGCTACG 
CTCACTCAAA CTGTGAAAAC ACAAGCCAAA GAAACTCTCG ATAAAGCCCA GAACAAGAAG 
GACATGTTTG AGAAGTCCAA CAAGATGCTC AAAAACTTCA TCCAGAAGAT TAAAGACTTT 
CTGAATGAGG AGGGAGCAGA TCCAGAAAGC ATTGAAAAAG TGGCCCAGCA GGTTCTGGCA 
ATCTCCTTGC CAGTCAACAG GACTGTAATA GTCAACTTAG TGGAGCAAAT CAAAGACAGC 
ATCGTCAACC TCACCAACGT GGAGGGAGTG TTCAATCACA CCTCAGAACA ACTGGCCAAA 
GTCAACGATC TACTCAAAAA AGCTCAGGAT GCAAAGACAC AAGCTGATGG AGTGAGTGAT 
AATATCAATA AAACAAAAGA AGCACTTGAA ACCTCACAGA ATGCCATAAA GAAAGCTGAA 
GAGGAAATGA GCACAGCTCT AAAAAACCTC AAGAATATCC AGAACATCAC TGCCATGGTT 
GACGACAAAC TCTTAAATTT AGACAACAAC CTTACAGATG TGATGATGCG TTTGGTCAAT 
TTGTCAAATG GAGTGGATTT ATTAAAGAAT AAAACGGAGC AGAACAGGGA GATGGCCAAA 
GAAGCCAAAG CCCAGTCGGA CAATGCAACT CGAGAAGCAG AAGGACTTCA AGAGGAGCTG 
ACAAATGCTG AGACTCTGTA CAAACAGCTG AAAGAGAAGG TGGACTCGGC TGGAGGGACA 
GGAGAAGGCA ATGTTAACCA GAAAGCCATA GAGATGAAGA AAGAAGCAGA AGATCTGCTG 
AAGAAGGCCA CCATGGGCAT GGAAACTCTC AAAAAATTGG AGAGAAAGTT CAACAAGAAC 
GAGCAGAAAA TGCAGCAGCA ACGGGATGAA CTGACAGATC TAGAGAAAAA TGTTACAGGC 
ATCAGAGAAT ATATCAGATC AAAAGTATTG GCCTACAACA ACTGTCAGTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

lamb2l ( XM_687746.9 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_692838.6 Danio rerio laminin, beta 2-like (lamb2l), transcript variant X1, mRNA.

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CloneID	ODa27654
Clone ID Related Accession (Same CDS sequence)	XM_687746.9 , XM_005162121.4 , XM_005162121.3 , XM_687746.8
Accession Version	XM_687746.9 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5511bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	laminin subunit beta-1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007134.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_687746.8. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGGAGA GATATTTCAG TGTTCTGCTT CTTGTTCTTC CTGCTTTGGC TCAGGATCTG 
CCCTCTGCCC CACATGGGTG CATGGATGGT AGCTGTTATC CAGCAACAGG CAACCTTCTG 
ATTGGCCGAG CTATAAACCT GACCACTTCC TCCACCTGTG GCCTGGATGG ACCCGAGCCA 
TACTGTATTG TCAGTCATTT GAAGGATTCA GAAAAGTGCT TTATATGTGA CTCTGAGAAT 
CCGTATGATG CCAATTATCA CAGAGACAGC CACAGAGTGG AGAACGTCAT CTACCCGATG 
GACAGCACAG GGGAACTCAC ATGGTGGCAG GCTGTAAATG GAGAGGAGAA TGTCAGTGTC 
AGACTGAATC TTGAGGCCGA GTTCCACTTT ACTCATCTCA TCATGAAGTT TAAGACATTT 
AGACCTGCTG CTATGATAAT TGAGCGTTCC TCTGATTTTG GCCGTACATG GAGGATATAC 
CGCTACTTTG CTTACAACTG CACTAAGACA TTTCCTAGGG TTCCTGCGCA CTCGCTGCGG 
TTCATTGATG AAGTCATTTG TGAAGAGCGC TACTCTGATA TTGAGCCATC CACTGATGGA 
GAGGTCATCT ACAAAGTGCT TGATCCTGCA ATCCATGTCA AAGATCCATA TAGTCTGGAC 
ATTCAGGATT TGTTGCGGAT CACTAACCTA CGGATAAACT TCACTAAACT GCACACTCTG 
GGTGATAACC TGCTTGACCG GCGTCCTGAC GTGCTGCAGA AGTATTACTA TGCATTGTAT 
GAGCTGGTTG TGCGCGGGAG CTGCTTCTGT TATGGCCATG CATCAGAGTG TGCACCAGTA 
CCTGGAGTGA CCACTAGAGA TTCAGGCATG ATTCATGGAC GCTGTGTCTG TAAGCACAAC 
ACTGTGGGAT TAAACTGTGA GCGCTGCAGA GATTTTTATC ATGATGCACC CTGGAGGCCT 
GCTGAGACCG ATAATGCACA CACCTGCAGG GAATGCAACT GTAATGGTCA CTCCAACCAG 
TGTCATTTTG ACATGGCGGT GTATTTGGCG ACGGGGAATG TCAGTGGAGG TGTGTGTAAC 
AACTGTCTTC ACAACACCAT GGGCCGCAAC TGTGAGAGCT GTAAGCCTTT CTACTACCAG 
GATCCCGAGA AAGACATCAG AGACCCGGCA GCGTGTGTCC CCTGTGACTG TGACCCCGTG 
GGCTCTGTGG AGGGAGGAGT GTGTGACAGT CATACAGATC TGGATTTGGG AATGATTGGT 
GGTCAGTGCC GTTGTAAGCA GAATGTCAGA GGTCAGCGCT GCGACTACTG TAAAGAGGGA 
CATTATGGTC TCAGTCTTGA TGACCCATTA GGATGCCAGC CTTGTAACTG TGACCCTCGT 
GGCATCACTA CACTGGGGGT TCCGTGTGAT CAGATTAGTG GGGACTGCTC TTGCAAAAGA 
TATGTAACAG GACGCTACTG CAACCAGTGC CTGCCTGAAT ATTGGGGACT CAGTCATGAT 
CTGGCTGGTT GCAGGCCCTG CAACTGTGAC TTTGGTGGAG CAACAAGCAG CAGATGCATG 
ATGGACAGCG GTCAGTGTGA CTGCCGGCCT CATCTGATTG GACGACAGTG TTCTGAAGTG 
CAGCCTGCAT TTTTCTGTGC TTCTTTGGAT TATTACAAGT ATGAAGCAGA AGCAGTTATG 
GGCCGCTCTC CTGAAGATCT CACACTTCCA GGCAGCCCAA GGCCTCAGGC TGAGACTGAC 
TGTGTGCAGC ATCTGAACAA TCAACTGAGA AGACACCGCC GTCACAGACA TATTGCACAA 
CAGCAGAGGG CAGCACTGAG ACGCATCCGA CAGCTGCAGC AAACACCCAG CGTGAGCTCA 
GTGCACAGAG AGAGACAGCC GGGTCAGATG GTCACCTGGA CCGGGCCGGG CTTCGCTCGG 
GTTAAAGACG GGGCTGGTCT TGAGTTTGCC ATCAGCAACA TCCCTTATGC CATGGAGTAT 
GACATTATGA TCCGATATGA GCCAGAGTCC ACAGAAGACT GGGAGGCTTT AGTGAGCGTC 
ACATCCGTGA ATTTACCCAT AAGCCTCCGC TGTGGCAACA TGCTGCCCAC AGAGCAGCTG 
TATACAGTCC CAATGCCCCA CAACGTGAGG TACATTAAAA TGCCAAAGCC CTTCTGCTTC 
GAGCCAAACA ACCGCTATGT GGTGTCCATT CGGTTCCAGC GCCACGCCGT GTCTCACCGC 
CATCTTACCG CCTTCATCCT GATAGACTCA CTTGTGCTGA TTCCCAGATA TGAAGAGCTT 
ACTGGATTTC AGGGTGATGA AGGTGCAGAT GAGCGTCGTC GTCAGGAGAT GGAGTCGTAC 
ATGTGTTTGG AGTCCTTCAT GACTATGCCA ATGCCAGCGC TGGCTGAACT GTGCACTTCT 
CTTATCTGCA GCATCTCTGC TATCATGCAC AATGGTGCAC TGGGGTGTCA GTGTGACCCT 
CAGGGTTCAT TAAGTGCAGA GTGTGACAGA GTCGGAGGTC AGTGTCGCTG TAAGCCCAAC 
GTGATTGGCC GTAAATGTAA TCAGTGTGCA CCGGGGACAT ATGGATTCGG CCCCTACGGC 
TGCACTGTAT GTGACTGTCA CTCGCAGGGT TCTGTCGGGC ATCATTGTGA CCCTGTGACG 
GGACAGTGTA TGTGTCGGCA TGGGGCGATT GGCCGCCAGT GCTCAGAATG CCAGCCGGGT 
CAGTGGGGAT TTCCTAACTG TAGGCAATGC CAGTGTAACA GCCATGCAGA GAGCTGCGAT 
CCTGAAAGCG GGGCCTGCTA TGACTGCAGA GACAACACAG CTGGACAACT GTGCGAGAGG 
TGTGTGGATG GTTATTATGG CAACCCAGCA CTTGGTTCTG GTGAGCACTG TCGCCCATGT 
CCGTGTCCAG GACACCCTGA CAGTGGACAT TCCAACGGTG CCTCCTGTGA AGTAGACTAC 
GCCTCCAACC AGATCCTTTG CCAGTGTGGG CAAGGCTACG CTGGTCCTCG CTGTGATCGC 
TGCGCCCCCG GTTATTATGG TGACCCCGAG CACCCTGGTG GATCGTGCCG GCCCTGCCAG 
TGCAATGGGA ACATTGACCC TCAGGACCCA GAGTCATGTG ACCCTCGAAC TGGACTGTGC 
CTCAAGTGTC TGTATAACAC AGATGGAGAC TCTTGCTCAG AGTGTAAACT GGGTTATTAT 
GGAAATGCAC TCATTCAGGA CTGTAGGCGA TGTACTTGTG TAACTGCAGG CACTTTGCCA 
AACTCTTGCA CTGATGGAGT GTGTCAATGT GATAAACAGA CAGGACAGTG CCCCTGCAAG 
CAAAACATCA TTGGACATAA CTGTGACCAG TGCGCCACCA ATCACTGGAA CTTCGGCTCT 
GACTGTGGAT GTGAACCCTG TGGATGTGTC CAACCTAATG CCCTCAGCAC CCACTGTAAC 
ATGTTTAGTG GCCAGTGTCA CTGTCAGCCA GGTTTTGGGG GGCGGCAGTG CAATGAGTGT 
GAGGCGTTCC ACTGGGGCGA TCCCAGAGTG CAATGTGAAG AGTGTAACTG CCATCCTCTG 
GGCTCTGAGA CGTCTCAGTG TGACCGCACA ACAGGCGCAT GTGTGTGTAA GGACGGAGCT 
TCAGGACAGC GCTGTGATGA GTGTGCACGC GGCTTTACTG GAAACTTCCC CAAATGTGTT 
CCCTGCCACC CCTGCTTCCA GCTGTGGGAT GATGTGGTGT GCCAGGTCCG CCGAGATCTG 
ACCCACTTCA GGGATATCAT TGCTATGATT CTGGAGAAGG GTGAGGTGCC TGGAGTCAGC 
GACAAGCGCA TACTTGAACT AGAGAAGAAG CTGGCACAGG CTCAAGAACT GGTCCGGGAT 
GGAGACAGAG AGGGAACATT TAACTTAATC AGCCAAACTG TTGATGACTT GAGAGCGGAG 
ATCGCTTTGA CTGATGGACG TCTGATGGGT GTAATACGGG ATCTGAATGT GACAACAGAC 
CATAACAATG ATCTGAAACA AAACCTAACT GCCCTGGAGA AAGAACTGAA AGATCTGAAC 
AATACGCTTC ACCAGCTGCG CAGAGAGCTG GAGAACTACC TCACTGCTGG ACTGGCAGAG 
CAATTTGCAA ATGTGCTGAA GTATTACCAG AGATCCCTGA ATTCAGAGCA GCGCTGCAAT 
GCCTCCGTAT ACGGACCCCA GAGTCCTGTG GAACAGTCTC AGGACACACG TAACCGTACC 
GAAGCCCTGC TGAAGGACAA GAAGGACAGC CTCCTGCGTA CAGCCACAGC TAACAATAAA 
TCCCTGTCTG AACTGGAGGG AAAAGCTCAT GAGATCAACC GAAAGGTCCA TCACCTTAGC 
AACAAGGTCT GCGGTGGCCA CGCCAATGGC AATGTGAATG GTAGCTGTGA AGAGAGCCCC 
TGTGGAGGAG CGGGATGTCG TGGCAGTGAT GGTGTGCTGA AGTGTGGAGG TAGAGGATGT 
AATGGAACTG TTGGTGCTTC AGTGAAGGCT TTAGACAATG CTGATGAGGT CCATAAGAAC 
CTGACTGCTG TTAGCGAGGA GCTCCAAACC ATGGCTAAGA AGCTACGAGA CATTGCTACG 
CTCACTCAAA CTGTGAAAAC ACAAGCCAAA GAAACTCTCG ATAAAGCCCA GAACAAGAAG 
GACATGTTTG AGAAGTCCAA CAAGATGCTC AAAAACTTCA TCCAGAAGAT TAAAGACTTT 
CTGAATGAGG AGGGAGCAGA TCCAGAAAGC ATTGAAAAAG TGGCCCAGCA GGTTCTGGCA 
ATCTCCTTGC CAGTCAACAG GACTGTAATA GTCAACTTAG TGGAGCAAAT CAAAGACAGC 
ATCGTCAACC TCACCAACGT GGAGGGAGTG TTCAATCACA CCTCAGAACA ACTGGCCAAA 
GTCAACGATC TACTCAAAAA AGCTCAGGAT GCAAAGACAC AAGCTGATGG AGTGAGTGAT 
AATATCAATA AAACAAAAGA AGCACTTGAA ACCTCACAGA ATGCCATAAA GAAAGCTGAA 
GAGGAAATGA GCACAGCTCT AAAAAACCTC AAGAATATCC AGAACATCAC TGCCATGGTT 
GACGACAAAC TCTTAAATTT AGACAACAAC CTTACAGATG TGATGATGCG TTTAGTCAAT 
TTGTCAAATG GAGTGGATTT ATTAAAGAAT AAAACGGAGC AGAACAGGGA GATGGCCAAA 
GAAGCCAAAG CCCAGTCGGA CAATGCAACT CGAGAAGCAG AGGGACTTCA AGAGGAGCTG 
ACAAATGCTG AGACTCTGTA CAAACAGCTG AAAGAGAAGG TGGACTCGGC TGGAGGGACA 
GGAGAAGGCA ATGTTAACCA GAAAGCCATA GAGATGAAGA AAGAAGCAGA AGATCTGCTG 
AAGAAGGCCA CCATGGGCAT GGAAACTCTC AAAAAATTGG AGAGAAAGTT CAACAAGAAC 
GAGCAGAAAA TGCAGCAGCA ACGGGATGAA CTGACAGATC TGGAGAAAAA TGTTACAGGC 
ATCAGAGAAT ATATCAGATC AAAAGTATTG GCCTACAACA ACTGTCAGTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_692838.6
CDS	289..5799
Translation	MMERYFSVLLLVLPALAQDLPSAPHGCMDGSCYPATGNLLIGRAINLTTSSTCGLDGPEPYCIVSHLKDSEKCFICDSENPYDANYHRDSHRVENVIYPMDSTGELTWWQAVNGEENVSVRLNLEAEFHFTHLIMKFKTFRPAAMIIERSSDFGRTWRIYRYFAYNCTKTFPRVPAHSLRFIDEVICEERYSDIEPSTDGEVIYKVLDPAIHVKDPYSLDIQDLLRITNLRINFTKLHTLGDNLLDRRPDVLQKYYYALYELVVRGSCFCYGHASECAPVPGVTTRDSGMIHGRCVCKHNTVGLNCERCRDFYHDAPWRPAETDNAHTCRECNCNGHSNQCHFDMAVYLATGNVSGGVCNNCLHNTMGRNCESCKPFYYQDPEKDIRDPAACVPCDCDPVGSVEGGVCDSHTDLDLGMIGGQCRCKQNVRGQRCDYCKEGHYGLSLDDPLGCQPCNCDPRGITTLGVPCDQISGDCSCKRYVTGRYCNQCLPEYWGLSHDLAGCRPCNCDFGGATSSRCMMDSGQCDCRPHLIGRQCSEVQPAFFCASLDYYKYEAEAVMGRSPEDLTLPGSPRPQAETDCVQHLNNQLRRHRRHRHIAQQQRAALRRIRQLQQTPSVSSVHRERQPGQMVTWTGPGFARVKDGAGLEFAISNIPYAMEYDIMIRYEPESTEDWEALVSVTSVNLPISLRCGNMLPTEQLYTVPMPHNVRYIKMPKPFCFEPNNRYVVSIRFQRHAVSHRHLTAFILIDSLVLIPRYEELTGFQGDEGADERRRQEMESYMCLESFMTMPMPALAELCTSLICSISAIMHNGALGCQCDPQGSLSAECDRVGGQCRCKPNVIGRKCNQCAPGTYGFGPYGCTVCDCHSQGSVGHHCDPVTGQCMCRHGAIGRQCSECQPGQWGFPNCRQCQCNSHAESCDPESGACYDCRDNTAGQLCERCVDGYYGNPALGSGEHCRPCPCPGHPDSGHSNGASCEVDYASNQILCQCGQGYAGPRCDRCAPGYYGDPEHPGGSCRPCQCNGNIDPQDPESCDPRTGLCLKCLYNTDGDSCSECKLGYYGNALIQDCRRCTCVTAGTLPNSCTDGVCQCDKQTGQCPCKQNIIGHNCDQCATNHWNFGSDCGCEPCGCVQPNALSTHCNMFSGQCHCQPGFGGRQCNECEAFHWGDPRVQCEECNCHPLGSETSQCDRTTGACVCKDGASGQRCDECARGFTGNFPKCVPCHPCFQLWDDVVCQVRRDLTHFRDIIAMILEKGEVPGVSDKRILELEKKLAQAQELVRDGDREGTFNLISQTVDDLRAEIALTDGRLMGVIRDLNVTTDHNNDLKQNLTALEKELKDLNNTLHQLRRELENYLTAGLAEQFANVLKYYQRSLNSEQRCNASVYGPQSPVEQSQDTRNRTEALLKDKKDSLLRTATANNKSLSELEGKAHEINRKVHHLSNKVCGGHANGNVNGSCEESPCGGAGCRGSDGVLKCGGRGCNGTVGASVKALDNADEVHKNLTAVSEELQTMAKKLRDIATLTQTVKTQAKETLDKAQNKKDMFEKSNKMLKNFIQKIKDFLNEEGADPESIEKVAQQVLAISLPVNRTVIVNLVEQIKDSIVNLTNVEGVFNHTSEQLAKVNDLLKKAQDAKTQADGVSDNINKTKEALETSQNAIKKAEEEMSTALKNLKNIQNITAMVDDKLLNLDNNLTDVMMRLVNLSNGVDLLKNKTEQNREMAKEAKAQSDNATREAEGLQEELTNAETLYKQLKEKVDSAGGTGEGNVNQKAIEMKKEAEDLLKKATMGMETLKKLERKFNKNEQKMQQQRDELTDLEKNVTGIREYIRSKVLAYNNCQ

Target ORF information:

RefSeq Version	XM_687746.9
Organism	Danio rerio(zebrafish)
Definition	Danio rerio laminin, beta 2-like (lamb2l), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_687746.9

ORF Insert Sequence:

ATGATGGAGA GATATTTCAG TGTTCTGCTT CTTGTTCTTC CTGCTTTGGC TCAGGATCTG 
CCCTCTGCCC CACATGGGTG CATGGATGGT AGCTGTTATC CAGCAACAGG CAACCTTCTG 
ATTGGCCGAG CTATAAACCT GACCACTTCC TCCACCTGTG GCCTGGATGG ACCCGAGCCA 
TACTGTATTG TCAGTCATTT GAAGGATTCA GAAAAGTGCT TTATATGTGA CTCTGAGAAT 
CCGTATGATG CCAATTATCA CAGAGACAGC CACAGAGTGG AGAACGTCAT CTACCCGATG 
GACAGCACAG GGGAACTCAC ATGGTGGCAG GCTGTAAATG GAGAGGAGAA TGTCAGTGTC 
AGACTGAATC TTGAGGCCGA GTTCCACTTT ACTCATCTCA TCATGAAGTT TAAGACATTT 
AGACCTGCTG CTATGATAAT TGAGCGTTCC TCTGATTTTG GCCGTACATG GAGGATATAC 
CGCTACTTTG CTTACAACTG CACTAAGACA TTTCCTAGGG TTCCTGCGCA CTCGCTGCGG 
TTCATTGATG AAGTCATTTG TGAAGAGCGC TACTCTGATA TTGAGCCATC CACTGATGGA 
GAGGTCATCT ACAAAGTGCT TGATCCTGCA ATCCATGTCA AAGATCCATA TAGTCTGGAC 
ATTCAGGATT TGTTGCGGAT CACTAACCTA CGGATAAACT TCACTAAACT GCACACTCTG 
GGTGATAACC TGCTTGACCG GCGTCCTGAC GTGCTGCAGA AGTATTACTA TGCATTGTAT 
GAGCTGGTTG TGCGCGGGAG CTGCTTCTGT TATGGCCATG CATCAGAGTG TGCACCAGTA 
CCTGGAGTGA CCACTAGAGA TTCAGGCATG ATTCATGGAC GCTGTGTCTG TAAGCACAAC 
ACTGTGGGAT TAAACTGTGA GCGCTGCAGA GATTTTTATC ATGATGCACC CTGGAGGCCT 
GCTGAGACCG ATAATGCACA CACCTGCAGG GAATGCAACT GTAATGGTCA CTCCAACCAG 
TGTCATTTTG ACATGGCGGT GTATTTGGCG ACGGGGAATG TCAGTGGAGG TGTGTGTAAC 
AACTGTCTTC ACAACACCAT GGGCCGCAAC TGTGAGAGCT GTAAGCCTTT CTACTACCAG 
GATCCCGAGA AAGACATCAG AGACCCGGCA GCGTGTGTCC CCTGTGACTG TGACCCCGTG 
GGCTCTGTGG AGGGAGGAGT GTGTGACAGT CATACAGATC TGGATTTGGG AATGATTGGT 
GGTCAGTGCC GTTGTAAGCA GAATGTCAGA GGTCAGCGCT GCGACTACTG TAAAGAGGGA 
CATTATGGTC TCAGTCTTGA TGACCCATTA GGATGCCAGC CTTGTAACTG TGACCCTCGT 
GGCATCACTA CACTGGGGGT TCCGTGTGAT CAGATTAGTG GGGACTGCTC TTGCAAAAGA 
TATGTAACAG GACGCTACTG CAACCAGTGC CTGCCTGAAT ATTGGGGACT CAGTCATGAT 
CTGGCTGGTT GCAGGCCCTG CAACTGTGAC TTTGGTGGAG CAACAAGCAG CAGATGCATG 
ATGGACAGCG GTCAGTGTGA CTGCCGGCCT CATCTGATTG GACGACAGTG TTCTGAAGTG 
CAGCCTGCAT TTTTCTGTGC TTCTTTGGAT TATTACAAGT ATGAAGCAGA AGCAGTTATG 
GGCCGCTCTC CTGAAGATCT CACACTTCCA GGCAGCCCAA GGCCTCAGGC TGAGACTGAC 
TGTGTGCAGC ATCTGAACAA TCAACTGAGA AGACACCGCC GTCACAGACA TATTGCACAA 
CAGCAGAGGG CAGCACTGAG ACGCATCCGA CAGCTGCAGC AAACACCCAG CGTGAGCTCA 
GTGCACAGAG AGAGACAGCC GGGTCAGATG GTCACCTGGA CCGGGCCGGG CTTCGCTCGG 
GTTAAAGACG GGGCTGGTCT TGAGTTTGCC ATCAGCAACA TCCCTTATGC CATGGAGTAT 
GACATTATGA TCCGATATGA GCCAGAGTCC ACAGAAGACT GGGAGGCTTT AGTGAGCGTC 
ACATCCGTGA ATTTACCCAT AAGCCTCCGC TGTGGCAACA TGCTGCCCAC AGAGCAGCTG 
TATACAGTCC CAATGCCCCA CAACGTGAGG TACATTAAAA TGCCAAAGCC CTTCTGCTTC 
GAGCCAAACA ACCGCTATGT GGTGTCCATT CGGTTCCAGC GCCACGCCGT GTCTCACCGC 
CATCTTACCG CCTTCATCCT GATAGACTCA CTTGTGCTGA TTCCCAGATA TGAAGAGCTT 
ACTGGATTTC AGGGTGATGA AGGTGCAGAT GAGCGTCGTC GTCAGGAGAT GGAGTCGTAC 
ATGTGTTTGG AGTCCTTCAT GACTATGCCA ATGCCAGCGC TGGCTGAACT GTGCACTTCT 
CTTATCTGCA GCATCTCTGC TATCATGCAC AATGGTGCAC TGGGGTGTCA GTGTGACCCT 
CAGGGTTCAT TAAGTGCAGA GTGTGACAGA GTCGGAGGTC AGTGTCGCTG TAAGCCCAAC 
GTGATTGGCC GTAAATGTAA TCAGTGTGCA CCGGGGACAT ATGGATTCGG CCCCTACGGC 
TGCACTGTAT GTGACTGTCA CTCGCAGGGT TCTGTCGGGC ATCATTGTGA CCCTGTGACG 
GGACAGTGTA TGTGTCGGCA TGGGGCGATT GGCCGCCAGT GCTCAGAATG CCAGCCGGGT 
CAGTGGGGAT TTCCTAACTG TAGGCAATGC CAGTGTAACA GCCATGCAGA GAGCTGCGAT 
CCTGAAAGCG GGGCCTGCTA TGACTGCAGA GACAACACAG CTGGACAACT GTGCGAGAGG 
TGTGTGGATG GTTATTATGG CAACCCAGCA CTTGGTTCTG GTGAGCACTG TCGCCCATGT 
CCGTGTCCAG GACACCCTGA CAGTGGACAT TCCAACGGTG CCTCCTGTGA AGTAGACTAC 
GCCTCCAACC AGATCCTTTG CCAGTGTGGG CAAGGCTACG CTGGTCCTCG CTGTGATCGC 
TGCGCCCCCG GTTATTATGG TGACCCCGAG CACCCTGGTG GATCGTGCCG GCCCTGCCAG 
TGCAATGGGA ACATTGACCC TCAGGACCCA GAGTCATGTG ACCCTCGAAC TGGACTGTGC 
CTCAAGTGTC TGTATAACAC AGATGGAGAC TCTTGCTCAG AGTGTAAACT GGGTTATTAT 
GGAAATGCAC TCATTCAGGA CTGTAGGCGA TGTACTTGTG TAACTGCAGG CACTTTGCCA 
AACTCTTGCA CTGATGGAGT GTGTCAATGT GATAAACAGA CAGGACAGTG CCCCTGCAAG 
CAAAACATCA TTGGACATAA CTGTGACCAG TGCGCCACCA ATCACTGGAA CTTCGGCTCT 
GACTGTGGAT GTGAACCCTG TGGATGTGTC CAACCTAATG CCCTCAGCAC CCACTGTAAC 
ATGTTTAGTG GCCAGTGTCA CTGTCAGCCA GGTTTTGGGG GGCGGCAGTG CAATGAGTGT 
GAGGCGTTCC ACTGGGGCGA TCCCAGAGTG CAATGTGAAG AGTGTAACTG CCATCCTCTG 
GGCTCTGAGA CGTCTCAGTG TGACCGCACA ACAGGCGCAT GTGTGTGTAA GGACGGAGCT 
TCAGGACAGC GCTGTGATGA GTGTGCACGC GGCTTTACTG GAAACTTCCC CAAATGTGTT 
CCCTGCCACC CCTGCTTCCA GCTGTGGGAT GATGTGGTGT GCCAGGTCCG CCGAGATCTG 
ACCCACTTCA GGGATATCAT TGCTATGATT CTGGAGAAGG GTGAGGTGCC TGGAGTCAGC 
GACAAGCGCA TACTTGAACT AGAGAAGAAG CTGGCACAGG CTCAAGAACT GGTCCGGGAT 
GGAGACAGAG AGGGAACATT TAACTTAATC AGCCAAACTG TTGATGACTT GAGAGCGGAG 
ATCGCTTTGA CTGATGGACG TCTGATGGGT GTAATACGGG ATCTGAATGT GACAACAGAC 
CATAACAATG ATCTGAAACA AAACCTAACT GCCCTGGAGA AAGAACTGAA AGATCTGAAC 
AATACGCTTC ACCAGCTGCG CAGAGAGCTG GAGAACTACC TCACTGCTGG ACTGGCAGAG 
CAATTTGCAA ATGTGCTGAA GTATTACCAG AGATCCCTGA ATTCAGAGCA GCGCTGCAAT 
GCCTCCGTAT ACGGACCCCA GAGTCCTGTG GAACAGTCTC AGGACACACG TAACCGTACC 
GAAGCCCTGC TGAAGGACAA GAAGGACAGC CTCCTGCGTA CAGCCACAGC TAACAATAAA 
TCCCTGTCTG AACTGGAGGG AAAAGCTCAT GAGATCAACC GAAAGGTCCA TCACCTTAGC 
AACAAGGTCT GCGGTGGCCA CGCCAATGGC AATGTGAATG GTAGCTGTGA AGAGAGCCCC 
TGTGGAGGAG CGGGATGTCG TGGCAGTGAT GGTGTGCTGA AGTGTGGAGG TAGAGGATGT 
AATGGAACTG TTGGTGCTTC AGTGAAGGCT TTAGACAATG CTGATGAGGT CCATAAGAAC 
CTGACTGCTG TTAGCGAGGA GCTCCAAACC ATGGCTAAGA AGCTACGAGA CATTGCTACG 
CTCACTCAAA CTGTGAAAAC ACAAGCCAAA GAAACTCTCG ATAAAGCCCA GAACAAGAAG 
GACATGTTTG AGAAGTCCAA CAAGATGCTC AAAAACTTCA TCCAGAAGAT TAAAGACTTT 
CTGAATGAGG AGGGAGCAGA TCCAGAAAGC ATTGAAAAAG TGGCCCAGCA GGTTCTGGCA 
ATCTCCTTGC CAGTCAACAG GACTGTAATA GTCAACTTAG TGGAGCAAAT CAAAGACAGC 
ATCGTCAACC TCACCAACGT GGAGGGAGTG TTCAATCACA CCTCAGAACA ACTGGCCAAA 
GTCAACGATC TACTCAAAAA AGCTCAGGAT GCAAAGACAC AAGCTGATGG AGTGAGTGAT 
AATATCAATA AAACAAAAGA AGCACTTGAA ACCTCACAGA ATGCCATAAA GAAAGCTGAA 
GAGGAAATGA GCACAGCTCT AAAAAACCTC AAGAATATCC AGAACATCAC TGCCATGGTT 
GACGACAAAC TCTTAAATTT AGACAACAAC CTTACAGATG TGATGATGCG TTTAGTCAAT 
TTGTCAAATG GAGTGGATTT ATTAAAGAAT AAAACGGAGC AGAACAGGGA GATGGCCAAA 
GAAGCCAAAG CCCAGTCGGA CAATGCAACT CGAGAAGCAG AGGGACTTCA AGAGGAGCTG 
ACAAATGCTG AGACTCTGTA CAAACAGCTG AAAGAGAAGG TGGACTCGGC TGGAGGGACA 
GGAGAAGGCA ATGTTAACCA GAAAGCCATA GAGATGAAGA AAGAAGCAGA AGATCTGCTG 
AAGAAGGCCA CCATGGGCAT GGAAACTCTC AAAAAATTGG AGAGAAAGTT CAACAAGAAC 
GAGCAGAAAA TGCAGCAGCA ACGGGATGAA CTGACAGATC TGGAGAAAAA TGTTACAGGC 
ATCAGAGAAT ATATCAGATC AAAAGTATTG GCCTACAACA ACTGTCAGTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

lamb2l ( XM_005162121.4 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005162178.1 Danio rerio laminin, beta 2-like (lamb2l), transcript variant X2, mRNA.

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CloneID	ODa27654
Clone ID Related Accession (Same CDS sequence)	XM_687746.9 , XM_005162121.4 , XM_005162121.3 , XM_687746.8
Accession Version	XM_005162121.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5511bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	laminin subunit beta-1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007134.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005162121.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGGAGA GATATTTCAG TGTTCTGCTT CTTGTTCTTC CTGCTTTGGC TCAGGATCTG 
CCCTCTGCCC CACATGGGTG CATGGATGGT AGCTGTTATC CAGCAACAGG CAACCTTCTG 
ATTGGCCGAG CTATAAACCT GACCACTTCC TCCACCTGTG GCCTGGATGG ACCCGAGCCA 
TACTGTATTG TCAGTCATTT GAAGGATTCA GAAAAGTGCT TTATATGTGA CTCTGAGAAT 
CCGTATGATG CCAATTATCA CAGAGACAGC CACAGAGTGG AGAACGTCAT CTACCCGATG 
GACAGCACAG GGGAACTCAC ATGGTGGCAG GCTGTAAATG GAGAGGAGAA TGTCAGTGTC 
AGACTGAATC TTGAGGCCGA GTTCCACTTT ACTCATCTCA TCATGAAGTT TAAGACATTT 
AGACCTGCTG CTATGATAAT TGAGCGTTCC TCTGATTTTG GCCGTACATG GAGGATATAC 
CGCTACTTTG CTTACAACTG CACTAAGACA TTTCCTAGGG TTCCTGCGCA CTCGCTGCGG 
TTCATTGATG AAGTCATTTG TGAAGAGCGC TACTCTGATA TTGAGCCATC CACTGATGGA 
GAGGTCATCT ACAAAGTGCT TGATCCTGCA ATCCATGTCA AAGATCCATA TAGTCTGGAC 
ATTCAGGATT TGTTGCGGAT CACTAACCTA CGGATAAACT TCACTAAACT GCACACTCTG 
GGTGATAACC TGCTTGACCG GCGTCCTGAC GTGCTGCAGA AGTATTACTA TGCATTGTAT 
GAGCTGGTTG TGCGCGGGAG CTGCTTCTGT TATGGCCATG CATCAGAGTG TGCACCAGTA 
CCTGGAGTGA CCACTAGAGA TTCAGGCATG ATTCATGGAC GCTGTGTCTG TAAGCACAAC 
ACTGTGGGAT TAAACTGTGA GCGCTGCAGA GATTTTTATC ATGATGCACC CTGGAGGCCT 
GCTGAGACCG ATAATGCACA CACCTGCAGG GAATGCAACT GTAATGGTCA CTCCAACCAG 
TGTCATTTTG ACATGGCGGT GTATTTGGCG ACGGGGAATG TCAGTGGAGG TGTGTGTAAC 
AACTGTCTTC ACAACACCAT GGGCCGCAAC TGTGAGAGCT GTAAGCCTTT CTACTACCAG 
GATCCCGAGA AAGACATCAG AGACCCGGCA GCGTGTGTCC CCTGTGACTG TGACCCCGTG 
GGCTCTGTGG AGGGAGGAGT GTGTGACAGT CATACAGATC TGGATTTGGG AATGATTGGT 
GGTCAGTGCC GTTGTAAGCA GAATGTCAGA GGTCAGCGCT GCGACTACTG TAAAGAGGGA 
CATTATGGTC TCAGTCTTGA TGACCCATTA GGATGCCAGC CTTGTAACTG TGACCCTCGT 
GGCATCACTA CACTGGGGGT TCCGTGTGAT CAGATTAGTG GGGACTGCTC TTGCAAAAGA 
TATGTAACAG GACGCTACTG CAACCAGTGC CTGCCTGAAT ATTGGGGACT CAGTCATGAT 
CTGGCTGGTT GCAGGCCCTG CAACTGTGAC TTTGGTGGAG CAACAAGCAG CAGATGCATG 
ATGGACAGCG GTCAGTGTGA CTGCCGGCCT CATCTGATTG GACGACAGTG TTCTGAAGTG 
CAGCCTGCAT TTTTCTGTGC TTCTTTGGAT TATTACAAGT ATGAAGCAGA AGCAGTTATG 
GGCCGCTCTC CTGAAGATCT CACACTTCCA GGCAGCCCAA GGCCTCAGGC TGAGACTGAC 
TGTGTGCAGC ATCTGAACAA TCAACTGAGA AGACACCGCC GTCACAGACA TATTGCACAA 
CAGCAGAGGG CAGCACTGAG ACGCATCCGA CAGCTGCAGC AAACACCCAG CGTGAGCTCA 
GTGCACAGAG AGAGACAGCC GGGTCAGATG GTCACCTGGA CCGGGCCGGG CTTCGCTCGG 
GTTAAAGACG GGGCTGGTCT TGAGTTTGCC ATCAGCAACA TCCCTTATGC CATGGAGTAT 
GACATTATGA TCCGATATGA GCCAGAGTCC ACAGAAGACT GGGAGGCTTT AGTGAGCGTC 
ACATCCGTGA ATTTACCCAT AAGCCTCCGC TGTGGCAACA TGCTGCCCAC AGAGCAGCTG 
TATACAGTCC CAATGCCCCA CAACGTGAGG TACATTAAAA TGCCAAAGCC CTTCTGCTTC 
GAGCCAAACA ACCGCTATGT GGTGTCCATT CGGTTCCAGC GCCACGCCGT GTCTCACCGC 
CATCTTACCG CCTTCATCCT GATAGACTCA CTTGTGCTGA TTCCCAGATA TGAAGAGCTT 
ACTGGATTTC AGGGTGATGA AGGTGCAGAT GAGCGTCGTC GTCAGGAGAT GGAGTCGTAC 
ATGTGTTTGG AGTCCTTCAT GACTATGCCA ATGCCAGCGC TGGCTGAACT GTGCACTTCT 
CTTATCTGCA GCATCTCTGC TATCATGCAC AATGGTGCAC TGGGGTGTCA GTGTGACCCT 
CAGGGTTCAT TAAGTGCAGA GTGTGACAGA GTCGGAGGTC AGTGTCGCTG TAAGCCCAAC 
GTGATTGGCC GTAAATGTAA TCAGTGTGCA CCGGGGACAT ATGGATTCGG CCCCTACGGC 
TGCACTGTAT GTGACTGTCA CTCGCAGGGT TCTGTCGGGC ATCATTGTGA CCCTGTGACG 
GGACAGTGTA TGTGTCGGCA TGGGGCGATT GGCCGCCAGT GCTCAGAATG CCAGCCGGGT 
CAGTGGGGAT TTCCTAACTG TAGGCAATGC CAGTGTAACA GCCATGCAGA GAGCTGCGAT 
CCTGAAAGCG GGGCCTGCTA TGACTGCAGA GACAACACAG CTGGACAACT GTGCGAGAGG 
TGTGTGGATG GTTATTATGG CAACCCAGCA CTTGGTTCTG GTGAGCACTG TCGCCCATGT 
CCGTGTCCAG GACACCCTGA CAGTGGACAT TCCAACGGTG CCTCCTGTGA AGTAGACTAC 
GCCTCCAACC AGATCCTTTG CCAGTGTGGG CAAGGCTACG CTGGTCCTCG CTGTGATCGC 
TGCGCCCCCG GTTATTATGG TGACCCCGAG CACCCTGGTG GATCGTGCCG GCCCTGCCAG 
TGCAATGGGA ACATTGACCC TCAGGACCCA GAGTCATGTG ACCCTCGAAC TGGACTGTGC 
CTCAAGTGTC TGTATAACAC AGATGGAGAC TCTTGCTCAG AGTGTAAACT GGGTTATTAT 
GGAAATGCAC TCATTCAGGA CTGTAGGCGA TGTACTTGTG TAACTGCAGG CACTTTGCCA 
AACTCTTGCA CTGATGGAGT GTGTCAATGT GATAAACAGA CAGGACAGTG CCCCTGCAAG 
CAAAACATCA TTGGACATAA CTGTGACCAG TGCGCCACCA ATCACTGGAA CTTCGGCTCT 
GACTGTGGAT GTGAACCCTG TGGATGTGTC CAACCTAATG CCCTCAGCAC CCACTGTAAC 
ATGTTTAGTG GCCAGTGTCA CTGTCAGCCA GGTTTTGGGG GGCGGCAGTG CAATGAGTGT 
GAGGCGTTCC ACTGGGGCGA TCCCAGAGTG CAATGTGAAG AGTGTAACTG CCATCCTCTG 
GGCTCTGAGA CGTCTCAGTG TGACCGCACA ACAGGCGCAT GTGTGTGTAA GGACGGAGCT 
TCAGGACAGC GCTGTGATGA GTGTGCACGC GGCTTTACTG GAAACTTCCC CAAATGTGTT 
CCCTGCCACC CCTGCTTCCA GCTGTGGGAT GATGTGGTGT GCCAGGTCCG CCGAGATCTG 
ACCCACTTCA GGGATATCAT TGCTATGATT CTGGAGAAGG GTGAGGTGCC TGGAGTCAGC 
GACAAGCGCA TACTTGAACT AGAGAAGAAG CTGGCACAGG CTCAAGAACT GGTCCGGGAT 
GGAGACAGAG AGGGAACATT TAACTTAATC AGCCAAACTG TTGATGACTT GAGAGCGGAG 
ATCGCTTTGA CTGATGGACG TCTGATGGGT GTAATACGGG ATCTGAATGT GACAACAGAC 
CATAACAATG ATCTGAAACA AAACCTAACT GCCCTGGAGA AAGAACTGAA AGATCTGAAC 
AATACGCTTC ACCAGCTGCG CAGAGAGCTG GAGAACTACC TCACTGCTGG ACTGGCAGAG 
CAATTTGCAA ATGTGCTGAA GTATTACCAG AGATCCCTGA ATTCAGAGCA GCGCTGCAAT 
GCCTCCGTAT ACGGACCCCA GAGTCCTGTG GAACAGTCTC AGGACACACG TAACCGTACC 
GAAGCCCTGC TGAAGGACAA GAAGGACAGC CTCCTGCGTA CAGCCACAGC TAACAATAAA 
TCCCTGTCTG AACTGGAGGG AAAAGCTCAT GAGATCAACC GAAAGGTCCA TCACCTTAGC 
AACAAGGTCT GCGGTGGCCA CGCCAATGGC AATGTGAATG GTAGCTGTGA AGAGAGCCCC 
TGTGGAGGAG CGGGATGTCG TGGCAGTGAT GGTGTGCTGA AGTGTGGAGG TAGAGGATGT 
AATGGAACTG TTGGTGCTTC AGTGAAGGCT TTAGACAATG CTGATGAGGT CCATAAGAAC 
CTGACTGCTG TTAGCGAGGA GCTCCAAACC ATGGCTAAGA AGCTACGAGA CATTGCTACG 
CTCACTCAAA CTGTGAAAAC ACAAGCCAAA GAAACTCTCG ATAAAGCCCA GAACAAGAAG 
GACATGTTTG AGAAGTCCAA CAAGATGCTC AAAAACTTCA TCCAGAAGAT TAAAGACTTT 
CTGAATGAGG AGGGAGCAGA TCCAGAAAGC ATTGAAAAAG TGGCCCAGCA GGTTCTGGCA 
ATCTCCTTGC CAGTCAACAG GACTGTAATA GTCAACTTAG TGGAGCAAAT CAAAGACAGC 
ATCGTCAACC TCACCAACGT GGAGGGAGTG TTCAATCACA CCTCAGAACA ACTGGCCAAA 
GTCAACGATC TACTCAAAAA AGCTCAGGAT GCAAAGACAC AAGCTGATGG AGTGAGTGAT 
AATATCAATA AAACAAAAGA AGCACTTGAA ACCTCACAGA ATGCCATAAA GAAAGCTGAA 
GAGGAAATGA GCACAGCTCT AAAAAACCTC AAGAATATCC AGAACATCAC TGCCATGGTT 
GACGACAAAC TCTTAAATTT AGACAACAAC CTTACAGATG TGATGATGCG TTTAGTCAAT 
TTGTCAAATG GAGTGGATTT ATTAAAGAAT AAAACGGAGC AGAACAGGGA GATGGCCAAA 
GAAGCCAAAG CCCAGTCGGA CAATGCAACT CGAGAAGCAG AGGGACTTCA AGAGGAGCTG 
ACAAATGCTG AGACTCTGTA CAAACAGCTG AAAGAGAAGG TGGACTCGGC TGGAGGGACA 
GGAGAAGGCA ATGTTAACCA GAAAGCCATA GAGATGAAGA AAGAAGCAGA AGATCTGCTG 
AAGAAGGCCA CCATGGGCAT GGAAACTCTC AAAAAATTGG AGAGAAAGTT CAACAAGAAC 
GAGCAGAAAA TGCAGCAGCA ACGGGATGAA CTGACAGATC TGGAGAAAAA TGTTACAGGC 
ATCAGAGAAT ATATCAGATC AAAAGTATTG GCCTACAACA ACTGTCAGTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005162178.1
CDS	315..5825
Translation	MMERYFSVLLLVLPALAQDLPSAPHGCMDGSCYPATGNLLIGRAINLTTSSTCGLDGPEPYCIVSHLKDSEKCFICDSENPYDANYHRDSHRVENVIYPMDSTGELTWWQAVNGEENVSVRLNLEAEFHFTHLIMKFKTFRPAAMIIERSSDFGRTWRIYRYFAYNCTKTFPRVPAHSLRFIDEVICEERYSDIEPSTDGEVIYKVLDPAIHVKDPYSLDIQDLLRITNLRINFTKLHTLGDNLLDRRPDVLQKYYYALYELVVRGSCFCYGHASECAPVPGVTTRDSGMIHGRCVCKHNTVGLNCERCRDFYHDAPWRPAETDNAHTCRECNCNGHSNQCHFDMAVYLATGNVSGGVCNNCLHNTMGRNCESCKPFYYQDPEKDIRDPAACVPCDCDPVGSVEGGVCDSHTDLDLGMIGGQCRCKQNVRGQRCDYCKEGHYGLSLDDPLGCQPCNCDPRGITTLGVPCDQISGDCSCKRYVTGRYCNQCLPEYWGLSHDLAGCRPCNCDFGGATSSRCMMDSGQCDCRPHLIGRQCSEVQPAFFCASLDYYKYEAEAVMGRSPEDLTLPGSPRPQAETDCVQHLNNQLRRHRRHRHIAQQQRAALRRIRQLQQTPSVSSVHRERQPGQMVTWTGPGFARVKDGAGLEFAISNIPYAMEYDIMIRYEPESTEDWEALVSVTSVNLPISLRCGNMLPTEQLYTVPMPHNVRYIKMPKPFCFEPNNRYVVSIRFQRHAVSHRHLTAFILIDSLVLIPRYEELTGFQGDEGADERRRQEMESYMCLESFMTMPMPALAELCTSLICSISAIMHNGALGCQCDPQGSLSAECDRVGGQCRCKPNVIGRKCNQCAPGTYGFGPYGCTVCDCHSQGSVGHHCDPVTGQCMCRHGAIGRQCSECQPGQWGFPNCRQCQCNSHAESCDPESGACYDCRDNTAGQLCERCVDGYYGNPALGSGEHCRPCPCPGHPDSGHSNGASCEVDYASNQILCQCGQGYAGPRCDRCAPGYYGDPEHPGGSCRPCQCNGNIDPQDPESCDPRTGLCLKCLYNTDGDSCSECKLGYYGNALIQDCRRCTCVTAGTLPNSCTDGVCQCDKQTGQCPCKQNIIGHNCDQCATNHWNFGSDCGCEPCGCVQPNALSTHCNMFSGQCHCQPGFGGRQCNECEAFHWGDPRVQCEECNCHPLGSETSQCDRTTGACVCKDGASGQRCDECARGFTGNFPKCVPCHPCFQLWDDVVCQVRRDLTHFRDIIAMILEKGEVPGVSDKRILELEKKLAQAQELVRDGDREGTFNLISQTVDDLRAEIALTDGRLMGVIRDLNVTTDHNNDLKQNLTALEKELKDLNNTLHQLRRELENYLTAGLAEQFANVLKYYQRSLNSEQRCNASVYGPQSPVEQSQDTRNRTEALLKDKKDSLLRTATANNKSLSELEGKAHEINRKVHHLSNKVCGGHANGNVNGSCEESPCGGAGCRGSDGVLKCGGRGCNGTVGASVKALDNADEVHKNLTAVSEELQTMAKKLRDIATLTQTVKTQAKETLDKAQNKKDMFEKSNKMLKNFIQKIKDFLNEEGADPESIEKVAQQVLAISLPVNRTVIVNLVEQIKDSIVNLTNVEGVFNHTSEQLAKVNDLLKKAQDAKTQADGVSDNINKTKEALETSQNAIKKAEEEMSTALKNLKNIQNITAMVDDKLLNLDNNLTDVMMRLVNLSNGVDLLKNKTEQNREMAKEAKAQSDNATREAEGLQEELTNAETLYKQLKEKVDSAGGTGEGNVNQKAIEMKKEAEDLLKKATMGMETLKKLERKFNKNEQKMQQQRDELTDLEKNVTGIREYIRSKVLAYNNCQ

Target ORF information:

RefSeq Version	XM_005162121.4
Organism	Danio rerio(zebrafish)
Definition	Danio rerio laminin, beta 2-like (lamb2l), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005162121.4

ORF Insert Sequence:

ATGATGGAGA GATATTTCAG TGTTCTGCTT CTTGTTCTTC CTGCTTTGGC TCAGGATCTG 
CCCTCTGCCC CACATGGGTG CATGGATGGT AGCTGTTATC CAGCAACAGG CAACCTTCTG 
ATTGGCCGAG CTATAAACCT GACCACTTCC TCCACCTGTG GCCTGGATGG ACCCGAGCCA 
TACTGTATTG TCAGTCATTT GAAGGATTCA GAAAAGTGCT TTATATGTGA CTCTGAGAAT 
CCGTATGATG CCAATTATCA CAGAGACAGC CACAGAGTGG AGAACGTCAT CTACCCGATG 
GACAGCACAG GGGAACTCAC ATGGTGGCAG GCTGTAAATG GAGAGGAGAA TGTCAGTGTC 
AGACTGAATC TTGAGGCCGA GTTCCACTTT ACTCATCTCA TCATGAAGTT TAAGACATTT 
AGACCTGCTG CTATGATAAT TGAGCGTTCC TCTGATTTTG GCCGTACATG GAGGATATAC 
CGCTACTTTG CTTACAACTG CACTAAGACA TTTCCTAGGG TTCCTGCGCA CTCGCTGCGG 
TTCATTGATG AAGTCATTTG TGAAGAGCGC TACTCTGATA TTGAGCCATC CACTGATGGA 
GAGGTCATCT ACAAAGTGCT TGATCCTGCA ATCCATGTCA AAGATCCATA TAGTCTGGAC 
ATTCAGGATT TGTTGCGGAT CACTAACCTA CGGATAAACT TCACTAAACT GCACACTCTG 
GGTGATAACC TGCTTGACCG GCGTCCTGAC GTGCTGCAGA AGTATTACTA TGCATTGTAT 
GAGCTGGTTG TGCGCGGGAG CTGCTTCTGT TATGGCCATG CATCAGAGTG TGCACCAGTA 
CCTGGAGTGA CCACTAGAGA TTCAGGCATG ATTCATGGAC GCTGTGTCTG TAAGCACAAC 
ACTGTGGGAT TAAACTGTGA GCGCTGCAGA GATTTTTATC ATGATGCACC CTGGAGGCCT 
GCTGAGACCG ATAATGCACA CACCTGCAGG GAATGCAACT GTAATGGTCA CTCCAACCAG 
TGTCATTTTG ACATGGCGGT GTATTTGGCG ACGGGGAATG TCAGTGGAGG TGTGTGTAAC 
AACTGTCTTC ACAACACCAT GGGCCGCAAC TGTGAGAGCT GTAAGCCTTT CTACTACCAG 
GATCCCGAGA AAGACATCAG AGACCCGGCA GCGTGTGTCC CCTGTGACTG TGACCCCGTG 
GGCTCTGTGG AGGGAGGAGT GTGTGACAGT CATACAGATC TGGATTTGGG AATGATTGGT 
GGTCAGTGCC GTTGTAAGCA GAATGTCAGA GGTCAGCGCT GCGACTACTG TAAAGAGGGA 
CATTATGGTC TCAGTCTTGA TGACCCATTA GGATGCCAGC CTTGTAACTG TGACCCTCGT 
GGCATCACTA CACTGGGGGT TCCGTGTGAT CAGATTAGTG GGGACTGCTC TTGCAAAAGA 
TATGTAACAG GACGCTACTG CAACCAGTGC CTGCCTGAAT ATTGGGGACT CAGTCATGAT 
CTGGCTGGTT GCAGGCCCTG CAACTGTGAC TTTGGTGGAG CAACAAGCAG CAGATGCATG 
ATGGACAGCG GTCAGTGTGA CTGCCGGCCT CATCTGATTG GACGACAGTG TTCTGAAGTG 
CAGCCTGCAT TTTTCTGTGC TTCTTTGGAT TATTACAAGT ATGAAGCAGA AGCAGTTATG 
GGCCGCTCTC CTGAAGATCT CACACTTCCA GGCAGCCCAA GGCCTCAGGC TGAGACTGAC 
TGTGTGCAGC ATCTGAACAA TCAACTGAGA AGACACCGCC GTCACAGACA TATTGCACAA 
CAGCAGAGGG CAGCACTGAG ACGCATCCGA CAGCTGCAGC AAACACCCAG CGTGAGCTCA 
GTGCACAGAG AGAGACAGCC GGGTCAGATG GTCACCTGGA CCGGGCCGGG CTTCGCTCGG 
GTTAAAGACG GGGCTGGTCT TGAGTTTGCC ATCAGCAACA TCCCTTATGC CATGGAGTAT 
GACATTATGA TCCGATATGA GCCAGAGTCC ACAGAAGACT GGGAGGCTTT AGTGAGCGTC 
ACATCCGTGA ATTTACCCAT AAGCCTCCGC TGTGGCAACA TGCTGCCCAC AGAGCAGCTG 
TATACAGTCC CAATGCCCCA CAACGTGAGG TACATTAAAA TGCCAAAGCC CTTCTGCTTC 
GAGCCAAACA ACCGCTATGT GGTGTCCATT CGGTTCCAGC GCCACGCCGT GTCTCACCGC 
CATCTTACCG CCTTCATCCT GATAGACTCA CTTGTGCTGA TTCCCAGATA TGAAGAGCTT 
ACTGGATTTC AGGGTGATGA AGGTGCAGAT GAGCGTCGTC GTCAGGAGAT GGAGTCGTAC 
ATGTGTTTGG AGTCCTTCAT GACTATGCCA ATGCCAGCGC TGGCTGAACT GTGCACTTCT 
CTTATCTGCA GCATCTCTGC TATCATGCAC AATGGTGCAC TGGGGTGTCA GTGTGACCCT 
CAGGGTTCAT TAAGTGCAGA GTGTGACAGA GTCGGAGGTC AGTGTCGCTG TAAGCCCAAC 
GTGATTGGCC GTAAATGTAA TCAGTGTGCA CCGGGGACAT ATGGATTCGG CCCCTACGGC 
TGCACTGTAT GTGACTGTCA CTCGCAGGGT TCTGTCGGGC ATCATTGTGA CCCTGTGACG 
GGACAGTGTA TGTGTCGGCA TGGGGCGATT GGCCGCCAGT GCTCAGAATG CCAGCCGGGT 
CAGTGGGGAT TTCCTAACTG TAGGCAATGC CAGTGTAACA GCCATGCAGA GAGCTGCGAT 
CCTGAAAGCG GGGCCTGCTA TGACTGCAGA GACAACACAG CTGGACAACT GTGCGAGAGG 
TGTGTGGATG GTTATTATGG CAACCCAGCA CTTGGTTCTG GTGAGCACTG TCGCCCATGT 
CCGTGTCCAG GACACCCTGA CAGTGGACAT TCCAACGGTG CCTCCTGTGA AGTAGACTAC 
GCCTCCAACC AGATCCTTTG CCAGTGTGGG CAAGGCTACG CTGGTCCTCG CTGTGATCGC 
TGCGCCCCCG GTTATTATGG TGACCCCGAG CACCCTGGTG GATCGTGCCG GCCCTGCCAG 
TGCAATGGGA ACATTGACCC TCAGGACCCA GAGTCATGTG ACCCTCGAAC TGGACTGTGC 
CTCAAGTGTC TGTATAACAC AGATGGAGAC TCTTGCTCAG AGTGTAAACT GGGTTATTAT 
GGAAATGCAC TCATTCAGGA CTGTAGGCGA TGTACTTGTG TAACTGCAGG CACTTTGCCA 
AACTCTTGCA CTGATGGAGT GTGTCAATGT GATAAACAGA CAGGACAGTG CCCCTGCAAG 
CAAAACATCA TTGGACATAA CTGTGACCAG TGCGCCACCA ATCACTGGAA CTTCGGCTCT 
GACTGTGGAT GTGAACCCTG TGGATGTGTC CAACCTAATG CCCTCAGCAC CCACTGTAAC 
ATGTTTAGTG GCCAGTGTCA CTGTCAGCCA GGTTTTGGGG GGCGGCAGTG CAATGAGTGT 
GAGGCGTTCC ACTGGGGCGA TCCCAGAGTG CAATGTGAAG AGTGTAACTG CCATCCTCTG 
GGCTCTGAGA CGTCTCAGTG TGACCGCACA ACAGGCGCAT GTGTGTGTAA GGACGGAGCT 
TCAGGACAGC GCTGTGATGA GTGTGCACGC GGCTTTACTG GAAACTTCCC CAAATGTGTT 
CCCTGCCACC CCTGCTTCCA GCTGTGGGAT GATGTGGTGT GCCAGGTCCG CCGAGATCTG 
ACCCACTTCA GGGATATCAT TGCTATGATT CTGGAGAAGG GTGAGGTGCC TGGAGTCAGC 
GACAAGCGCA TACTTGAACT AGAGAAGAAG CTGGCACAGG CTCAAGAACT GGTCCGGGAT 
GGAGACAGAG AGGGAACATT TAACTTAATC AGCCAAACTG TTGATGACTT GAGAGCGGAG 
ATCGCTTTGA CTGATGGACG TCTGATGGGT GTAATACGGG ATCTGAATGT GACAACAGAC 
CATAACAATG ATCTGAAACA AAACCTAACT GCCCTGGAGA AAGAACTGAA AGATCTGAAC 
AATACGCTTC ACCAGCTGCG CAGAGAGCTG GAGAACTACC TCACTGCTGG ACTGGCAGAG 
CAATTTGCAA ATGTGCTGAA GTATTACCAG AGATCCCTGA ATTCAGAGCA GCGCTGCAAT 
GCCTCCGTAT ACGGACCCCA GAGTCCTGTG GAACAGTCTC AGGACACACG TAACCGTACC 
GAAGCCCTGC TGAAGGACAA GAAGGACAGC CTCCTGCGTA CAGCCACAGC TAACAATAAA 
TCCCTGTCTG AACTGGAGGG AAAAGCTCAT GAGATCAACC GAAAGGTCCA TCACCTTAGC 
AACAAGGTCT GCGGTGGCCA CGCCAATGGC AATGTGAATG GTAGCTGTGA AGAGAGCCCC 
TGTGGAGGAG CGGGATGTCG TGGCAGTGAT GGTGTGCTGA AGTGTGGAGG TAGAGGATGT 
AATGGAACTG TTGGTGCTTC AGTGAAGGCT TTAGACAATG CTGATGAGGT CCATAAGAAC 
CTGACTGCTG TTAGCGAGGA GCTCCAAACC ATGGCTAAGA AGCTACGAGA CATTGCTACG 
CTCACTCAAA CTGTGAAAAC ACAAGCCAAA GAAACTCTCG ATAAAGCCCA GAACAAGAAG 
GACATGTTTG AGAAGTCCAA CAAGATGCTC AAAAACTTCA TCCAGAAGAT TAAAGACTTT 
CTGAATGAGG AGGGAGCAGA TCCAGAAAGC ATTGAAAAAG TGGCCCAGCA GGTTCTGGCA 
ATCTCCTTGC CAGTCAACAG GACTGTAATA GTCAACTTAG TGGAGCAAAT CAAAGACAGC 
ATCGTCAACC TCACCAACGT GGAGGGAGTG TTCAATCACA CCTCAGAACA ACTGGCCAAA 
GTCAACGATC TACTCAAAAA AGCTCAGGAT GCAAAGACAC AAGCTGATGG AGTGAGTGAT 
AATATCAATA AAACAAAAGA AGCACTTGAA ACCTCACAGA ATGCCATAAA GAAAGCTGAA 
GAGGAAATGA GCACAGCTCT AAAAAACCTC AAGAATATCC AGAACATCAC TGCCATGGTT 
GACGACAAAC TCTTAAATTT AGACAACAAC CTTACAGATG TGATGATGCG TTTAGTCAAT 
TTGTCAAATG GAGTGGATTT ATTAAAGAAT AAAACGGAGC AGAACAGGGA GATGGCCAAA 
GAAGCCAAAG CCCAGTCGGA CAATGCAACT CGAGAAGCAG AGGGACTTCA AGAGGAGCTG 
ACAAATGCTG AGACTCTGTA CAAACAGCTG AAAGAGAAGG TGGACTCGGC TGGAGGGACA 
GGAGAAGGCA ATGTTAACCA GAAAGCCATA GAGATGAAGA AAGAAGCAGA AGATCTGCTG 
AAGAAGGCCA CCATGGGCAT GGAAACTCTC AAAAAATTGG AGAGAAAGTT CAACAAGAAC 
GAGCAGAAAA TGCAGCAGCA ACGGGATGAA CTGACAGATC TGGAGAAAAA TGTTACAGGC 
ATCAGAGAAT ATATCAGATC AAAAGTATTG GCCTACAACA ACTGTCAGTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

lamb2l ( XM_005162121.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005162178.1 Danio rerio laminin, beta 2-like (lamb2l), transcript variant X2, mRNA.

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CloneID	ODa27654
Clone ID Related Accession (Same CDS sequence)	XM_687746.9 , XM_005162121.4 , XM_005162121.3 , XM_687746.8
Accession Version	XM_005162121.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5511bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	laminin subunit beta-1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007134.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005162121.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGGAGA GATATTTCAG TGTTCTGCTT CTTGTTCTTC CTGCTTTGGC TCAGGATCTG 
CCCTCTGCCC CACATGGGTG CATGGATGGT AGCTGTTATC CAGCAACAGG CAACCTTCTG 
ATTGGCCGAG CTATAAACCT GACCACTTCC TCCACCTGTG GCCTGGATGG ACCCGAGCCA 
TACTGTATTG TCAGTCATTT GAAGGATTCA GAAAAGTGCT TTATATGTGA CTCTGAGAAT 
CCGTATGATG CCAATTATCA CAGAGACAGC CACAGAGTGG AGAACGTCAT CTACCCGATG 
GACAGCACAG GGGAACTCAC ATGGTGGCAG GCTGTAAATG GAGAGGAGAA TGTCAGTGTC 
AGACTGAATC TTGAGGCCGA GTTCCACTTT ACTCATCTCA TCATGAAGTT TAAGACATTT 
AGACCTGCTG CTATGATAAT TGAGCGTTCC TCTGATTTTG GCCGTACATG GAGGATATAC 
CGCTACTTTG CTTACAACTG CACTAAGACA TTTCCTAGGG TTCCTGCGCA CTCGCTGCGG 
TTCATTGATG AAGTCATTTG TGAAGAGCGC TACTCTGATA TTGAGCCATC CACTGATGGA 
GAGGTCATCT ACAAAGTGCT TGATCCTGCA ATCCATGTCA AAGATCCATA TAGTCTGGAC 
ATTCAGGATT TGTTGCGGAT CACTAACCTA CGGATAAACT TCACTAAACT GCACACTCTG 
GGTGATAACC TGCTTGACCG GCGTCCTGAC GTGCTGCAGA AGTATTACTA TGCATTGTAT 
GAGCTGGTTG TGCGCGGGAG CTGCTTCTGT TATGGCCATG CATCAGAGTG TGCACCAGTA 
CCTGGAGTGA CCACTAGAGA TTCAGGCATG ATTCATGGAC GCTGTGTCTG TAAGCACAAC 
ACTGTGGGAT TAAACTGTGA GCGCTGCAGA GATTTTTATC ATGATGCACC CTGGAGGCCT 
GCTGAGACCG ATAATGCACA CACCTGCAGG GAATGCAACT GTAATGGTCA CTCCAACCAG 
TGTCATTTTG ACATGGCGGT GTATTTGGCG ACGGGGAATG TCAGTGGAGG TGTGTGTAAC 
AACTGTCTTC ACAACACCAT GGGCCGCAAC TGTGAGAGCT GTAAGCCTTT CTACTACCAG 
GATCCCGAGA AAGACATCAG AGACCCGGCA GCGTGTGTCC CCTGTGACTG TGACCCCGTG 
GGCTCTGTGG AGGGAGGAGT GTGTGACAGT CATACAGATC TGGATTTGGG AATGATTGGT 
GGTCAGTGCC GTTGTAAGCA GAATGTCAGA GGTCAGCGCT GCGACTACTG TAAAGAGGGA 
CATTATGGTC TCAGTCTTGA TGACCCATTA GGATGCCAGC CTTGTAACTG TGACCCTCGT 
GGCATCACTA CACTGGGGGT TCCGTGTGAT CAGATTAGTG GGGACTGCTC TTGCAAAAGA 
TATGTAACAG GACGCTACTG CAACCAGTGC CTGCCTGAAT ATTGGGGACT CAGTCATGAT 
CTGGCTGGTT GCAGGCCCTG CAACTGTGAC TTTGGTGGAG CAACAAGCAG CAGATGCATG 
ATGGACAGCG GTCAGTGTGA CTGCCGGCCT CATCTGATTG GACGACAGTG TTCTGAAGTG 
CAGCCTGCAT TTTTCTGTGC TTCTTTGGAT TATTACAAGT ATGAAGCAGA AGCAGTTATG 
GGCCGCTCTC CTGAAGATCT CACACTTCCA GGCAGCCCAA GGCCTCAGGC TGAGACTGAC 
TGTGTGCAGC ATCTGAACAA TCAACTGAGA AGACACCGCC GTCACAGACA TATTGCACAA 
CAGCAGAGGG CAGCACTGAG ACGCATCCGA CAGCTGCAGC AAACACCCAG CGTGAGCTCA 
GTGCACAGAG AGAGACAGCC GGGTCAGATG GTCACCTGGA CCGGGCCGGG CTTCGCTCGG 
GTTAAAGACG GGGCTGGTCT TGAGTTTGCC ATCAGCAACA TCCCTTATGC CATGGAGTAT 
GACATTATGA TCCGATATGA GCCAGAGTCC ACAGAAGACT GGGAGGCTTT AGTGAGCGTC 
ACATCCGTGA ATTTACCCAT AAGCCTCCGC TGTGGCAACA TGCTGCCCAC AGAGCAGCTG 
TATACAGTCC CAATGCCCCA CAACGTGAGG TACATTAAAA TGCCAAAGCC CTTCTGCTTC 
GAGCCAAACA ACCGCTATGT GGTGTCCATT CGGTTCCAGC GCCACGCCGT GTCTCACCGC 
CATCTTACCG CCTTCATCCT GATAGACTCA CTTGTGCTGA TTCCCAGATA TGAAGAGCTT 
ACTGGATTTC AGGGTGATGA AGGTGCAGAT GAGCGTCGTC GTCAGGAGAT GGAGTCGTAC 
ATGTGTTTGG AGTCCTTCAT GACTATGCCA ATGCCAGCGC TGGCTGAACT GTGCACTTCT 
CTTATCTGCA GCATCTCTGC TATCATGCAC AATGGTGCAC TGGGGTGTCA GTGTGACCCT 
CAGGGTTCAT TAAGTGCAGA GTGTGACAGA GTCGGAGGTC AGTGTCGCTG TAAGCCCAAC 
GTGATTGGCC GTAAATGTAA TCAGTGTGCA CCGGGGACAT ATGGATTCGG CCCCTACGGC 
TGCACTGTAT GTGACTGTCA CTCGCAGGGT TCTGTCGGGC ATCATTGTGA CCCTGTGACG 
GGACAGTGTA TGTGTCGGCA TGGGGCGATT GGCCGCCAGT GCTCAGAATG CCAGCCGGGT 
CAGTGGGGAT TTCCTAACTG TAGGCAATGC CAGTGTAACA GCCATGCAGA GAGCTGCGAT 
CCTGAAAGCG GGGCCTGCTA TGACTGCAGA GACAACACAG CTGGACAACT GTGCGAGAGG 
TGTGTGGATG GTTATTATGG CAACCCAGCA CTTGGTTCTG GTGAGCACTG TCGCCCATGT 
CCGTGTCCAG GACACCCTGA CAGTGGACAT TCCAACGGTG CCTCCTGTGA AGTAGACTAC 
GCCTCCAACC AGATCCTTTG CCAGTGTGGG CAAGGCTACG CTGGTCCTCG CTGTGATCGC 
TGCGCCCCCG GTTATTATGG TGACCCCGAG CACCCTGGTG GATCGTGCCG GCCCTGCCAG 
TGCAATGGGA ACATTGACCC TCAGGACCCA GAGTCATGTG ACCCTCGAAC TGGACTGTGC 
CTCAAGTGTC TGTATAACAC AGATGGAGAC TCTTGCTCAG AGTGTAAACT GGGTTATTAT 
GGAAATGCAC TCATTCAGGA CTGTAGGCGA TGTACTTGTG TAACTGCAGG CACTTTGCCA 
AACTCTTGCA CTGATGGAGT GTGTCAATGT GATAAACAGA CAGGACAGTG CCCCTGCAAG 
CAAAACATCA TTGGACATAA CTGTGACCAG TGCGCCACCA ATCACTGGAA CTTCGGCTCT 
GACTGTGGAT GTGAACCCTG TGGATGTGTC CAACCTAATG CCCTCAGCAC CCACTGTAAC 
ATGTTTAGTG GCCAGTGTCA CTGTCAGCCA GGTTTTGGGG GGCGGCAGTG CAATGAGTGT 
GAGGCGTTCC ACTGGGGCGA TCCCAGAGTG CAATGTGAAG AGTGTAACTG CCATCCTCTG 
GGCTCTGAGA CGTCTCAGTG TGACCGCACA ACAGGCGCAT GTGTGTGTAA GGACGGAGCT 
TCAGGACAGC GCTGTGATGA GTGTGCACGC GGCTTTACTG GAAACTTCCC CAAATGTGTT 
CCCTGCCACC CCTGCTTCCA GCTGTGGGAT GATGTGGTGT GCCAGGTCCG CCGAGATCTG 
ACCCACTTCA GGGATATCAT TGCTATGATT CTGGAGAAGG GTGAGGTGCC TGGAGTCAGC 
GACAAGCGCA TACTTGAACT AGAGAAGAAG CTGGCACAGG CTCAAGAACT GGTCCGGGAT 
GGAGACAGAG AGGGAACATT TAACTTAATC AGCCAAACTG TTGATGACTT GAGAGCGGAG 
ATCGCTTTGA CTGATGGACG TCTGATGGGT GTAATACGGG ATCTGAATGT GACAACAGAC 
CATAACAATG ATCTGAAACA AAACCTAACT GCCCTGGAGA AAGAACTGAA AGATCTGAAC 
AATACGCTTC ACCAGCTGCG CAGAGAGCTG GAGAACTACC TCACTGCTGG ACTGGCAGAG 
CAATTTGCAA ATGTGCTGAA GTATTACCAG AGATCCCTGA ATTCAGAGCA GCGCTGCAAT 
GCCTCCGTAT ACGGACCCCA GAGTCCTGTG GAACAGTCTC AGGACACACG TAACCGTACC 
GAAGCCCTGC TGAAGGACAA GAAGGACAGC CTCCTGCGTA CAGCCACAGC TAACAATAAA 
TCCCTGTCTG AACTGGAGGG AAAAGCTCAT GAGATCAACC GAAAGGTCCA TCACCTTAGC 
AACAAGGTCT GCGGTGGCCA CGCCAATGGC AATGTGAATG GTAGCTGTGA AGAGAGCCCC 
TGTGGAGGAG CGGGATGTCG TGGCAGTGAT GGTGTGCTGA AGTGTGGAGG TAGAGGATGT 
AATGGAACTG TTGGTGCTTC AGTGAAGGCT TTAGACAATG CTGATGAGGT CCATAAGAAC 
CTGACTGCTG TTAGCGAGGA GCTCCAAACC ATGGCTAAGA AGCTACGAGA CATTGCTACG 
CTCACTCAAA CTGTGAAAAC ACAAGCCAAA GAAACTCTCG ATAAAGCCCA GAACAAGAAG 
GACATGTTTG AGAAGTCCAA CAAGATGCTC AAAAACTTCA TCCAGAAGAT TAAAGACTTT 
CTGAATGAGG AGGGAGCAGA TCCAGAAAGC ATTGAAAAAG TGGCCCAGCA GGTTCTGGCA 
ATCTCCTTGC CAGTCAACAG GACTGTAATA GTCAACTTAG TGGAGCAAAT CAAAGACAGC 
ATCGTCAACC TCACCAACGT GGAGGGAGTG TTCAATCACA CCTCAGAACA ACTGGCCAAA 
GTCAACGATC TACTCAAAAA AGCTCAGGAT GCAAAGACAC AAGCTGATGG AGTGAGTGAT 
AATATCAATA AAACAAAAGA AGCACTTGAA ACCTCACAGA ATGCCATAAA GAAAGCTGAA 
GAGGAAATGA GCACAGCTCT AAAAAACCTC AAGAATATCC AGAACATCAC TGCCATGGTT 
GACGACAAAC TCTTAAATTT AGACAACAAC CTTACAGATG TGATGATGCG TTTAGTCAAT 
TTGTCAAATG GAGTGGATTT ATTAAAGAAT AAAACGGAGC AGAACAGGGA GATGGCCAAA 
GAAGCCAAAG CCCAGTCGGA CAATGCAACT CGAGAAGCAG AGGGACTTCA AGAGGAGCTG 
ACAAATGCTG AGACTCTGTA CAAACAGCTG AAAGAGAAGG TGGACTCGGC TGGAGGGACA 
GGAGAAGGCA ATGTTAACCA GAAAGCCATA GAGATGAAGA AAGAAGCAGA AGATCTGCTG 
AAGAAGGCCA CCATGGGCAT GGAAACTCTC AAAAAATTGG AGAGAAAGTT CAACAAGAAC 
GAGCAGAAAA TGCAGCAGCA ACGGGATGAA CTGACAGATC TGGAGAAAAA TGTTACAGGC 
ATCAGAGAAT ATATCAGATC AAAAGTATTG GCCTACAACA ACTGTCAGTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005162178.1
CDS	180..5690
Translation	MMERYFSVLLLVLPALAQDLPSAPHGCMDGSCYPATGNLLIGRAINLTTSSTCGLDGPEPYCIVSHLKDSEKCFICDSENPYDANYHRDSHRVENVIYPMDSTGELTWWQAVNGEENVSVRLNLEAEFHFTHLIMKFKTFRPAAMIIERSSDFGRTWRIYRYFAYNCTKTFPRVPAHSLRFIDEVICEERYSDIEPSTDGEVIYKVLDPAIHVKDPYSLDIQDLLRITNLRINFTKLHTLGDNLLDRRPDVLQKYYYALYELVVRGSCFCYGHASECAPVPGVTTRDSGMIHGRCVCKHNTVGLNCERCRDFYHDAPWRPAETDNAHTCRECNCNGHSNQCHFDMAVYLATGNVSGGVCNNCLHNTMGRNCESCKPFYYQDPEKDIRDPAACVPCDCDPVGSVEGGVCDSHTDLDLGMIGGQCRCKQNVRGQRCDYCKEGHYGLSLDDPLGCQPCNCDPRGITTLGVPCDQISGDCSCKRYVTGRYCNQCLPEYWGLSHDLAGCRPCNCDFGGATSSRCMMDSGQCDCRPHLIGRQCSEVQPAFFCASLDYYKYEAEAVMGRSPEDLTLPGSPRPQAETDCVQHLNNQLRRHRRHRHIAQQQRAALRRIRQLQQTPSVSSVHRERQPGQMVTWTGPGFARVKDGAGLEFAISNIPYAMEYDIMIRYEPESTEDWEALVSVTSVNLPISLRCGNMLPTEQLYTVPMPHNVRYIKMPKPFCFEPNNRYVVSIRFQRHAVSHRHLTAFILIDSLVLIPRYEELTGFQGDEGADERRRQEMESYMCLESFMTMPMPALAELCTSLICSISAIMHNGALGCQCDPQGSLSAECDRVGGQCRCKPNVIGRKCNQCAPGTYGFGPYGCTVCDCHSQGSVGHHCDPVTGQCMCRHGAIGRQCSECQPGQWGFPNCRQCQCNSHAESCDPESGACYDCRDNTAGQLCERCVDGYYGNPALGSGEHCRPCPCPGHPDSGHSNGASCEVDYASNQILCQCGQGYAGPRCDRCAPGYYGDPEHPGGSCRPCQCNGNIDPQDPESCDPRTGLCLKCLYNTDGDSCSECKLGYYGNALIQDCRRCTCVTAGTLPNSCTDGVCQCDKQTGQCPCKQNIIGHNCDQCATNHWNFGSDCGCEPCGCVQPNALSTHCNMFSGQCHCQPGFGGRQCNECEAFHWGDPRVQCEECNCHPLGSETSQCDRTTGACVCKDGASGQRCDECARGFTGNFPKCVPCHPCFQLWDDVVCQVRRDLTHFRDIIAMILEKGEVPGVSDKRILELEKKLAQAQELVRDGDREGTFNLISQTVDDLRAEIALTDGRLMGVIRDLNVTTDHNNDLKQNLTALEKELKDLNNTLHQLRRELENYLTAGLAEQFANVLKYYQRSLNSEQRCNASVYGPQSPVEQSQDTRNRTEALLKDKKDSLLRTATANNKSLSELEGKAHEINRKVHHLSNKVCGGHANGNVNGSCEESPCGGAGCRGSDGVLKCGGRGCNGTVGASVKALDNADEVHKNLTAVSEELQTMAKKLRDIATLTQTVKTQAKETLDKAQNKKDMFEKSNKMLKNFIQKIKDFLNEEGADPESIEKVAQQVLAISLPVNRTVIVNLVEQIKDSIVNLTNVEGVFNHTSEQLAKVNDLLKKAQDAKTQADGVSDNINKTKEALETSQNAIKKAEEEMSTALKNLKNIQNITAMVDDKLLNLDNNLTDVMMRLVNLSNGVDLLKNKTEQNREMAKEAKAQSDNATREAEGLQEELTNAETLYKQLKEKVDSAGGTGEGNVNQKAIEMKKEAEDLLKKATMGMETLKKLERKFNKNEQKMQQQRDELTDLEKNVTGIREYIRSKVLAYNNCQ

Target ORF information:

RefSeq Version	XM_005162121.3
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio laminin, beta 2-like (lamb2l), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005162121.3

ORF Insert Sequence:

ATGATGGAGA GATATTTCAG TGTTCTGCTT CTTGTTCTTC CTGCTTTGGC TCAGGATCTG 
CCCTCTGCCC CACATGGGTG CATGGATGGT AGCTGTTATC CAGCAACAGG CAACCTTCTG 
ATTGGCCGAG CTATAAACCT GACCACTTCC TCCACCTGTG GCCTGGATGG ACCCGAGCCA 
TACTGTATTG TCAGTCATTT GAAGGATTCA GAAAAGTGCT TTATATGTGA CTCTGAGAAT 
CCGTATGATG CCAATTATCA CAGAGACAGC CACAGAGTGG AGAACGTCAT CTACCCGATG 
GACAGCACAG GGGAACTCAC ATGGTGGCAG GCTGTAAATG GAGAGGAGAA TGTCAGTGTC 
AGACTGAATC TTGAGGCCGA GTTCCACTTT ACTCATCTCA TCATGAAGTT TAAGACATTT 
AGACCTGCTG CTATGATAAT TGAGCGTTCC TCTGATTTTG GCCGTACATG GAGGATATAC 
CGCTACTTTG CTTACAACTG CACTAAGACA TTTCCTAGGG TTCCTGCGCA CTCGCTGCGG 
TTCATTGATG AAGTCATTTG TGAAGAGCGC TACTCTGATA TTGAGCCATC CACTGATGGA 
GAGGTCATCT ACAAAGTGCT TGATCCTGCA ATCCATGTCA AAGATCCATA TAGTCTGGAC 
ATTCAGGATT TGTTGCGGAT CACTAACCTA CGGATAAACT TCACTAAACT GCACACTCTG 
GGTGATAACC TGCTTGACCG GCGTCCTGAC GTGCTGCAGA AGTATTACTA TGCATTGTAT 
GAGCTGGTTG TGCGCGGGAG CTGCTTCTGT TATGGCCATG CATCAGAGTG TGCACCAGTA 
CCTGGAGTGA CCACTAGAGA TTCAGGCATG ATTCATGGAC GCTGTGTCTG TAAGCACAAC 
ACTGTGGGAT TAAACTGTGA GCGCTGCAGA GATTTTTATC ATGATGCACC CTGGAGGCCT 
GCTGAGACCG ATAATGCACA CACCTGCAGG GAATGCAACT GTAATGGTCA CTCCAACCAG 
TGTCATTTTG ACATGGCGGT GTATTTGGCG ACGGGGAATG TCAGTGGAGG TGTGTGTAAC 
AACTGTCTTC ACAACACCAT GGGCCGCAAC TGTGAGAGCT GTAAGCCTTT CTACTACCAG 
GATCCCGAGA AAGACATCAG AGACCCGGCA GCGTGTGTCC CCTGTGACTG TGACCCCGTG 
GGCTCTGTGG AGGGAGGAGT GTGTGACAGT CATACAGATC TGGATTTGGG AATGATTGGT 
GGTCAGTGCC GTTGTAAGCA GAATGTCAGA GGTCAGCGCT GCGACTACTG TAAAGAGGGA 
CATTATGGTC TCAGTCTTGA TGACCCATTA GGATGCCAGC CTTGTAACTG TGACCCTCGT 
GGCATCACTA CACTGGGGGT TCCGTGTGAT CAGATTAGTG GGGACTGCTC TTGCAAAAGA 
TATGTAACAG GACGCTACTG CAACCAGTGC CTGCCTGAAT ATTGGGGACT CAGTCATGAT 
CTGGCTGGTT GCAGGCCCTG CAACTGTGAC TTTGGTGGAG CAACAAGCAG CAGATGCATG 
ATGGACAGCG GTCAGTGTGA CTGCCGGCCT CATCTGATTG GACGACAGTG TTCTGAAGTG 
CAGCCTGCAT TTTTCTGTGC TTCTTTGGAT TATTACAAGT ATGAAGCAGA AGCAGTTATG 
GGCCGCTCTC CTGAAGATCT CACACTTCCA GGCAGCCCAA GGCCTCAGGC TGAGACTGAC 
TGTGTGCAGC ATCTGAACAA TCAACTGAGA AGACACCGCC GTCACAGACA TATTGCACAA 
CAGCAGAGGG CAGCACTGAG ACGCATCCGA CAGCTGCAGC AAACACCCAG CGTGAGCTCA 
GTGCACAGAG AGAGACAGCC GGGTCAGATG GTCACCTGGA CCGGGCCGGG CTTCGCTCGG 
GTTAAAGACG GGGCTGGTCT TGAGTTTGCC ATCAGCAACA TCCCTTATGC CATGGAGTAT 
GACATTATGA TCCGATATGA GCCAGAGTCC ACAGAAGACT GGGAGGCTTT AGTGAGCGTC 
ACATCCGTGA ATTTACCCAT AAGCCTCCGC TGTGGCAACA TGCTGCCCAC AGAGCAGCTG 
TATACAGTCC CAATGCCCCA CAACGTGAGG TACATTAAAA TGCCAAAGCC CTTCTGCTTC 
GAGCCAAACA ACCGCTATGT GGTGTCCATT CGGTTCCAGC GCCACGCCGT GTCTCACCGC 
CATCTTACCG CCTTCATCCT GATAGACTCA CTTGTGCTGA TTCCCAGATA TGAAGAGCTT 
ACTGGATTTC AGGGTGATGA AGGTGCAGAT GAGCGTCGTC GTCAGGAGAT GGAGTCGTAC 
ATGTGTTTGG AGTCCTTCAT GACTATGCCA ATGCCAGCGC TGGCTGAACT GTGCACTTCT 
CTTATCTGCA GCATCTCTGC TATCATGCAC AATGGTGCAC TGGGGTGTCA GTGTGACCCT 
CAGGGTTCAT TAAGTGCAGA GTGTGACAGA GTCGGAGGTC AGTGTCGCTG TAAGCCCAAC 
GTGATTGGCC GTAAATGTAA TCAGTGTGCA CCGGGGACAT ATGGATTCGG CCCCTACGGC 
TGCACTGTAT GTGACTGTCA CTCGCAGGGT TCTGTCGGGC ATCATTGTGA CCCTGTGACG 
GGACAGTGTA TGTGTCGGCA TGGGGCGATT GGCCGCCAGT GCTCAGAATG CCAGCCGGGT 
CAGTGGGGAT TTCCTAACTG TAGGCAATGC CAGTGTAACA GCCATGCAGA GAGCTGCGAT 
CCTGAAAGCG GGGCCTGCTA TGACTGCAGA GACAACACAG CTGGACAACT GTGCGAGAGG 
TGTGTGGATG GTTATTATGG CAACCCAGCA CTTGGTTCTG GTGAGCACTG TCGCCCATGT 
CCGTGTCCAG GACACCCTGA CAGTGGACAT TCCAACGGTG CCTCCTGTGA AGTAGACTAC 
GCCTCCAACC AGATCCTTTG CCAGTGTGGG CAAGGCTACG CTGGTCCTCG CTGTGATCGC 
TGCGCCCCCG GTTATTATGG TGACCCCGAG CACCCTGGTG GATCGTGCCG GCCCTGCCAG 
TGCAATGGGA ACATTGACCC TCAGGACCCA GAGTCATGTG ACCCTCGAAC TGGACTGTGC 
CTCAAGTGTC TGTATAACAC AGATGGAGAC TCTTGCTCAG AGTGTAAACT GGGTTATTAT 
GGAAATGCAC TCATTCAGGA CTGTAGGCGA TGTACTTGTG TAACTGCAGG CACTTTGCCA 
AACTCTTGCA CTGATGGAGT GTGTCAATGT GATAAACAGA CAGGACAGTG CCCCTGCAAG 
CAAAACATCA TTGGACATAA CTGTGACCAG TGCGCCACCA ATCACTGGAA CTTCGGCTCT 
GACTGTGGAT GTGAACCCTG TGGATGTGTC CAACCTAATG CCCTCAGCAC CCACTGTAAC 
ATGTTTAGTG GCCAGTGTCA CTGTCAGCCA GGTTTTGGGG GGCGGCAGTG CAATGAGTGT 
GAGGCGTTCC ACTGGGGCGA TCCCAGAGTG CAATGTGAAG AGTGTAACTG CCATCCTCTG 
GGCTCTGAGA CGTCTCAGTG TGACCGCACA ACAGGCGCAT GTGTGTGTAA GGACGGAGCT 
TCAGGACAGC GCTGTGATGA GTGTGCACGC GGCTTTACTG GAAACTTCCC CAAATGTGTT 
CCCTGCCACC CCTGCTTCCA GCTGTGGGAT GATGTGGTGT GCCAGGTCCG CCGAGATCTG 
ACCCACTTCA GGGATATCAT TGCTATGATT CTGGAGAAGG GTGAGGTGCC TGGAGTCAGC 
GACAAGCGCA TACTTGAACT AGAGAAGAAG CTGGCACAGG CTCAAGAACT GGTCCGGGAT 
GGAGACAGAG AGGGAACATT TAACTTAATC AGCCAAACTG TTGATGACTT GAGAGCGGAG 
ATCGCTTTGA CTGATGGACG TCTGATGGGT GTAATACGGG ATCTGAATGT GACAACAGAC 
CATAACAATG ATCTGAAACA AAACCTAACT GCCCTGGAGA AAGAACTGAA AGATCTGAAC 
AATACGCTTC ACCAGCTGCG CAGAGAGCTG GAGAACTACC TCACTGCTGG ACTGGCAGAG 
CAATTTGCAA ATGTGCTGAA GTATTACCAG AGATCCCTGA ATTCAGAGCA GCGCTGCAAT 
GCCTCCGTAT ACGGACCCCA GAGTCCTGTG GAACAGTCTC AGGACACACG TAACCGTACC 
GAAGCCCTGC TGAAGGACAA GAAGGACAGC CTCCTGCGTA CAGCCACAGC TAACAATAAA 
TCCCTGTCTG AACTGGAGGG AAAAGCTCAT GAGATCAACC GAAAGGTCCA TCACCTTAGC 
AACAAGGTCT GCGGTGGCCA CGCCAATGGC AATGTGAATG GTAGCTGTGA AGAGAGCCCC 
TGTGGAGGAG CGGGATGTCG TGGCAGTGAT GGTGTGCTGA AGTGTGGAGG TAGAGGATGT 
AATGGAACTG TTGGTGCTTC AGTGAAGGCT TTAGACAATG CTGATGAGGT CCATAAGAAC 
CTGACTGCTG TTAGCGAGGA GCTCCAAACC ATGGCTAAGA AGCTACGAGA CATTGCTACG 
CTCACTCAAA CTGTGAAAAC ACAAGCCAAA GAAACTCTCG ATAAAGCCCA GAACAAGAAG 
GACATGTTTG AGAAGTCCAA CAAGATGCTC AAAAACTTCA TCCAGAAGAT TAAAGACTTT 
CTGAATGAGG AGGGAGCAGA TCCAGAAAGC ATTGAAAAAG TGGCCCAGCA GGTTCTGGCA 
ATCTCCTTGC CAGTCAACAG GACTGTAATA GTCAACTTAG TGGAGCAAAT CAAAGACAGC 
ATCGTCAACC TCACCAACGT GGAGGGAGTG TTCAATCACA CCTCAGAACA ACTGGCCAAA 
GTCAACGATC TACTCAAAAA AGCTCAGGAT GCAAAGACAC AAGCTGATGG AGTGAGTGAT 
AATATCAATA AAACAAAAGA AGCACTTGAA ACCTCACAGA ATGCCATAAA GAAAGCTGAA 
GAGGAAATGA GCACAGCTCT AAAAAACCTC AAGAATATCC AGAACATCAC TGCCATGGTT 
GACGACAAAC TCTTAAATTT AGACAACAAC CTTACAGATG TGATGATGCG TTTAGTCAAT 
TTGTCAAATG GAGTGGATTT ATTAAAGAAT AAAACGGAGC AGAACAGGGA GATGGCCAAA 
GAAGCCAAAG CCCAGTCGGA CAATGCAACT CGAGAAGCAG AGGGACTTCA AGAGGAGCTG 
ACAAATGCTG AGACTCTGTA CAAACAGCTG AAAGAGAAGG TGGACTCGGC TGGAGGGACA 
GGAGAAGGCA ATGTTAACCA GAAAGCCATA GAGATGAAGA AAGAAGCAGA AGATCTGCTG 
AAGAAGGCCA CCATGGGCAT GGAAACTCTC AAAAAATTGG AGAGAAAGTT CAACAAGAAC 
GAGCAGAAAA TGCAGCAGCA ACGGGATGAA CTGACAGATC TGGAGAAAAA TGTTACAGGC 
ATCAGAGAAT ATATCAGATC AAAAGTATTG GCCTACAACA ACTGTCAGTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

lamb2l ( XM_687746.8 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_692838.6 Danio rerio laminin, beta 2-like (lamb2l), transcript variant X1, mRNA.

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CloneID	ODa27654
Clone ID Related Accession (Same CDS sequence)	XM_687746.9 , XM_005162121.4 , XM_005162121.3 , XM_687746.8
Accession Version	XM_687746.8	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5511bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	laminin subunit beta-1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007134.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_687746.7. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGGAGA GATATTTCAG TGTTCTGCTT CTTGTTCTTC CTGCTTTGGC TCAGGATCTG 
CCCTCTGCCC CACATGGGTG CATGGATGGT AGCTGTTATC CAGCAACAGG CAACCTTCTG 
ATTGGCCGAG CTATAAACCT GACCACTTCC TCCACCTGTG GCCTGGATGG ACCCGAGCCA 
TACTGTATTG TCAGTCATTT GAAGGATTCA GAAAAGTGCT TTATATGTGA CTCTGAGAAT 
CCGTATGATG CCAATTATCA CAGAGACAGC CACAGAGTGG AGAACGTCAT CTACCCGATG 
GACAGCACAG GGGAACTCAC ATGGTGGCAG GCTGTAAATG GAGAGGAGAA TGTCAGTGTC 
AGACTGAATC TTGAGGCCGA GTTCCACTTT ACTCATCTCA TCATGAAGTT TAAGACATTT 
AGACCTGCTG CTATGATAAT TGAGCGTTCC TCTGATTTTG GCCGTACATG GAGGATATAC 
CGCTACTTTG CTTACAACTG CACTAAGACA TTTCCTAGGG TTCCTGCGCA CTCGCTGCGG 
TTCATTGATG AAGTCATTTG TGAAGAGCGC TACTCTGATA TTGAGCCATC CACTGATGGA 
GAGGTCATCT ACAAAGTGCT TGATCCTGCA ATCCATGTCA AAGATCCATA TAGTCTGGAC 
ATTCAGGATT TGTTGCGGAT CACTAACCTA CGGATAAACT TCACTAAACT GCACACTCTG 
GGTGATAACC TGCTTGACCG GCGTCCTGAC GTGCTGCAGA AGTATTACTA TGCATTGTAT 
GAGCTGGTTG TGCGCGGGAG CTGCTTCTGT TATGGCCATG CATCAGAGTG TGCACCAGTA 
CCTGGAGTGA CCACTAGAGA TTCAGGCATG ATTCATGGAC GCTGTGTCTG TAAGCACAAC 
ACTGTGGGAT TAAACTGTGA GCGCTGCAGA GATTTTTATC ATGATGCACC CTGGAGGCCT 
GCTGAGACCG ATAATGCACA CACCTGCAGG GAATGCAACT GTAATGGTCA CTCCAACCAG 
TGTCATTTTG ACATGGCGGT GTATTTGGCG ACGGGGAATG TCAGTGGAGG TGTGTGTAAC 
AACTGTCTTC ACAACACCAT GGGCCGCAAC TGTGAGAGCT GTAAGCCTTT CTACTACCAG 
GATCCCGAGA AAGACATCAG AGACCCGGCA GCGTGTGTCC CCTGTGACTG TGACCCCGTG 
GGCTCTGTGG AGGGAGGAGT GTGTGACAGT CATACAGATC TGGATTTGGG AATGATTGGT 
GGTCAGTGCC GTTGTAAGCA GAATGTCAGA GGTCAGCGCT GCGACTACTG TAAAGAGGGA 
CATTATGGTC TCAGTCTTGA TGACCCATTA GGATGCCAGC CTTGTAACTG TGACCCTCGT 
GGCATCACTA CACTGGGGGT TCCGTGTGAT CAGATTAGTG GGGACTGCTC TTGCAAAAGA 
TATGTAACAG GACGCTACTG CAACCAGTGC CTGCCTGAAT ATTGGGGACT CAGTCATGAT 
CTGGCTGGTT GCAGGCCCTG CAACTGTGAC TTTGGTGGAG CAACAAGCAG CAGATGCATG 
ATGGACAGCG GTCAGTGTGA CTGCCGGCCT CATCTGATTG GACGACAGTG TTCTGAAGTG 
CAGCCTGCAT TTTTCTGTGC TTCTTTGGAT TATTACAAGT ATGAAGCAGA AGCAGTTATG 
GGCCGCTCTC CTGAAGATCT CACACTTCCA GGCAGCCCAA GGCCTCAGGC TGAGACTGAC 
TGTGTGCAGC ATCTGAACAA TCAACTGAGA AGACACCGCC GTCACAGACA TATTGCACAA 
CAGCAGAGGG CAGCACTGAG ACGCATCCGA CAGCTGCAGC AAACACCCAG CGTGAGCTCA 
GTGCACAGAG AGAGACAGCC GGGTCAGATG GTCACCTGGA CCGGGCCGGG CTTCGCTCGG 
GTTAAAGACG GGGCTGGTCT TGAGTTTGCC ATCAGCAACA TCCCTTATGC CATGGAGTAT 
GACATTATGA TCCGATATGA GCCAGAGTCC ACAGAAGACT GGGAGGCTTT AGTGAGCGTC 
ACATCCGTGA ATTTACCCAT AAGCCTCCGC TGTGGCAACA TGCTGCCCAC AGAGCAGCTG 
TATACAGTCC CAATGCCCCA CAACGTGAGG TACATTAAAA TGCCAAAGCC CTTCTGCTTC 
GAGCCAAACA ACCGCTATGT GGTGTCCATT CGGTTCCAGC GCCACGCCGT GTCTCACCGC 
CATCTTACCG CCTTCATCCT GATAGACTCA CTTGTGCTGA TTCCCAGATA TGAAGAGCTT 
ACTGGATTTC AGGGTGATGA AGGTGCAGAT GAGCGTCGTC GTCAGGAGAT GGAGTCGTAC 
ATGTGTTTGG AGTCCTTCAT GACTATGCCA ATGCCAGCGC TGGCTGAACT GTGCACTTCT 
CTTATCTGCA GCATCTCTGC TATCATGCAC AATGGTGCAC TGGGGTGTCA GTGTGACCCT 
CAGGGTTCAT TAAGTGCAGA GTGTGACAGA GTCGGAGGTC AGTGTCGCTG TAAGCCCAAC 
GTGATTGGCC GTAAATGTAA TCAGTGTGCA CCGGGGACAT ATGGATTCGG CCCCTACGGC 
TGCACTGTAT GTGACTGTCA CTCGCAGGGT TCTGTCGGGC ATCATTGTGA CCCTGTGACG 
GGACAGTGTA TGTGTCGGCA TGGGGCGATT GGCCGCCAGT GCTCAGAATG CCAGCCGGGT 
CAGTGGGGAT TTCCTAACTG TAGGCAATGC CAGTGTAACA GCCATGCAGA GAGCTGCGAT 
CCTGAAAGCG GGGCCTGCTA TGACTGCAGA GACAACACAG CTGGACAACT GTGCGAGAGG 
TGTGTGGATG GTTATTATGG CAACCCAGCA CTTGGTTCTG GTGAGCACTG TCGCCCATGT 
CCGTGTCCAG GACACCCTGA CAGTGGACAT TCCAACGGTG CCTCCTGTGA AGTAGACTAC 
GCCTCCAACC AGATCCTTTG CCAGTGTGGG CAAGGCTACG CTGGTCCTCG CTGTGATCGC 
TGCGCCCCCG GTTATTATGG TGACCCCGAG CACCCTGGTG GATCGTGCCG GCCCTGCCAG 
TGCAATGGGA ACATTGACCC TCAGGACCCA GAGTCATGTG ACCCTCGAAC TGGACTGTGC 
CTCAAGTGTC TGTATAACAC AGATGGAGAC TCTTGCTCAG AGTGTAAACT GGGTTATTAT 
GGAAATGCAC TCATTCAGGA CTGTAGGCGA TGTACTTGTG TAACTGCAGG CACTTTGCCA 
AACTCTTGCA CTGATGGAGT GTGTCAATGT GATAAACAGA CAGGACAGTG CCCCTGCAAG 
CAAAACATCA TTGGACATAA CTGTGACCAG TGCGCCACCA ATCACTGGAA CTTCGGCTCT 
GACTGTGGAT GTGAACCCTG TGGATGTGTC CAACCTAATG CCCTCAGCAC CCACTGTAAC 
ATGTTTAGTG GCCAGTGTCA CTGTCAGCCA GGTTTTGGGG GGCGGCAGTG CAATGAGTGT 
GAGGCGTTCC ACTGGGGCGA TCCCAGAGTG CAATGTGAAG AGTGTAACTG CCATCCTCTG 
GGCTCTGAGA CGTCTCAGTG TGACCGCACA ACAGGCGCAT GTGTGTGTAA GGACGGAGCT 
TCAGGACAGC GCTGTGATGA GTGTGCACGC GGCTTTACTG GAAACTTCCC CAAATGTGTT 
CCCTGCCACC CCTGCTTCCA GCTGTGGGAT GATGTGGTGT GCCAGGTCCG CCGAGATCTG 
ACCCACTTCA GGGATATCAT TGCTATGATT CTGGAGAAGG GTGAGGTGCC TGGAGTCAGC 
GACAAGCGCA TACTTGAACT AGAGAAGAAG CTGGCACAGG CTCAAGAACT GGTCCGGGAT 
GGAGACAGAG AGGGAACATT TAACTTAATC AGCCAAACTG TTGATGACTT GAGAGCGGAG 
ATCGCTTTGA CTGATGGACG TCTGATGGGT GTAATACGGG ATCTGAATGT GACAACAGAC 
CATAACAATG ATCTGAAACA AAACCTAACT GCCCTGGAGA AAGAACTGAA AGATCTGAAC 
AATACGCTTC ACCAGCTGCG CAGAGAGCTG GAGAACTACC TCACTGCTGG ACTGGCAGAG 
CAATTTGCAA ATGTGCTGAA GTATTACCAG AGATCCCTGA ATTCAGAGCA GCGCTGCAAT 
GCCTCCGTAT ACGGACCCCA GAGTCCTGTG GAACAGTCTC AGGACACACG TAACCGTACC 
GAAGCCCTGC TGAAGGACAA GAAGGACAGC CTCCTGCGTA CAGCCACAGC TAACAATAAA 
TCCCTGTCTG AACTGGAGGG AAAAGCTCAT GAGATCAACC GAAAGGTCCA TCACCTTAGC 
AACAAGGTCT GCGGTGGCCA CGCCAATGGC AATGTGAATG GTAGCTGTGA AGAGAGCCCC 
TGTGGAGGAG CGGGATGTCG TGGCAGTGAT GGTGTGCTGA AGTGTGGAGG TAGAGGATGT 
AATGGAACTG TTGGTGCTTC AGTGAAGGCT TTAGACAATG CTGATGAGGT CCATAAGAAC 
CTGACTGCTG TTAGCGAGGA GCTCCAAACC ATGGCTAAGA AGCTACGAGA CATTGCTACG 
CTCACTCAAA CTGTGAAAAC ACAAGCCAAA GAAACTCTCG ATAAAGCCCA GAACAAGAAG 
GACATGTTTG AGAAGTCCAA CAAGATGCTC AAAAACTTCA TCCAGAAGAT TAAAGACTTT 
CTGAATGAGG AGGGAGCAGA TCCAGAAAGC ATTGAAAAAG TGGCCCAGCA GGTTCTGGCA 
ATCTCCTTGC CAGTCAACAG GACTGTAATA GTCAACTTAG TGGAGCAAAT CAAAGACAGC 
ATCGTCAACC TCACCAACGT GGAGGGAGTG TTCAATCACA CCTCAGAACA ACTGGCCAAA 
GTCAACGATC TACTCAAAAA AGCTCAGGAT GCAAAGACAC AAGCTGATGG AGTGAGTGAT 
AATATCAATA AAACAAAAGA AGCACTTGAA ACCTCACAGA ATGCCATAAA GAAAGCTGAA 
GAGGAAATGA GCACAGCTCT AAAAAACCTC AAGAATATCC AGAACATCAC TGCCATGGTT 
GACGACAAAC TCTTAAATTT AGACAACAAC CTTACAGATG TGATGATGCG TTTAGTCAAT 
TTGTCAAATG GAGTGGATTT ATTAAAGAAT AAAACGGAGC AGAACAGGGA GATGGCCAAA 
GAAGCCAAAG CCCAGTCGGA CAATGCAACT CGAGAAGCAG AGGGACTTCA AGAGGAGCTG 
ACAAATGCTG AGACTCTGTA CAAACAGCTG AAAGAGAAGG TGGACTCGGC TGGAGGGACA 
GGAGAAGGCA ATGTTAACCA GAAAGCCATA GAGATGAAGA AAGAAGCAGA AGATCTGCTG 
AAGAAGGCCA CCATGGGCAT GGAAACTCTC AAAAAATTGG AGAGAAAGTT CAACAAGAAC 
GAGCAGAAAA TGCAGCAGCA ACGGGATGAA CTGACAGATC TGGAGAAAAA TGTTACAGGC 
ATCAGAGAAT ATATCAGATC AAAAGTATTG GCCTACAACA ACTGTCAGTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_692838.6
CDS	304..5814
Translation	MMERYFSVLLLVLPALAQDLPSAPHGCMDGSCYPATGNLLIGRAINLTTSSTCGLDGPEPYCIVSHLKDSEKCFICDSENPYDANYHRDSHRVENVIYPMDSTGELTWWQAVNGEENVSVRLNLEAEFHFTHLIMKFKTFRPAAMIIERSSDFGRTWRIYRYFAYNCTKTFPRVPAHSLRFIDEVICEERYSDIEPSTDGEVIYKVLDPAIHVKDPYSLDIQDLLRITNLRINFTKLHTLGDNLLDRRPDVLQKYYYALYELVVRGSCFCYGHASECAPVPGVTTRDSGMIHGRCVCKHNTVGLNCERCRDFYHDAPWRPAETDNAHTCRECNCNGHSNQCHFDMAVYLATGNVSGGVCNNCLHNTMGRNCESCKPFYYQDPEKDIRDPAACVPCDCDPVGSVEGGVCDSHTDLDLGMIGGQCRCKQNVRGQRCDYCKEGHYGLSLDDPLGCQPCNCDPRGITTLGVPCDQISGDCSCKRYVTGRYCNQCLPEYWGLSHDLAGCRPCNCDFGGATSSRCMMDSGQCDCRPHLIGRQCSEVQPAFFCASLDYYKYEAEAVMGRSPEDLTLPGSPRPQAETDCVQHLNNQLRRHRRHRHIAQQQRAALRRIRQLQQTPSVSSVHRERQPGQMVTWTGPGFARVKDGAGLEFAISNIPYAMEYDIMIRYEPESTEDWEALVSVTSVNLPISLRCGNMLPTEQLYTVPMPHNVRYIKMPKPFCFEPNNRYVVSIRFQRHAVSHRHLTAFILIDSLVLIPRYEELTGFQGDEGADERRRQEMESYMCLESFMTMPMPALAELCTSLICSISAIMHNGALGCQCDPQGSLSAECDRVGGQCRCKPNVIGRKCNQCAPGTYGFGPYGCTVCDCHSQGSVGHHCDPVTGQCMCRHGAIGRQCSECQPGQWGFPNCRQCQCNSHAESCDPESGACYDCRDNTAGQLCERCVDGYYGNPALGSGEHCRPCPCPGHPDSGHSNGASCEVDYASNQILCQCGQGYAGPRCDRCAPGYYGDPEHPGGSCRPCQCNGNIDPQDPESCDPRTGLCLKCLYNTDGDSCSECKLGYYGNALIQDCRRCTCVTAGTLPNSCTDGVCQCDKQTGQCPCKQNIIGHNCDQCATNHWNFGSDCGCEPCGCVQPNALSTHCNMFSGQCHCQPGFGGRQCNECEAFHWGDPRVQCEECNCHPLGSETSQCDRTTGACVCKDGASGQRCDECARGFTGNFPKCVPCHPCFQLWDDVVCQVRRDLTHFRDIIAMILEKGEVPGVSDKRILELEKKLAQAQELVRDGDREGTFNLISQTVDDLRAEIALTDGRLMGVIRDLNVTTDHNNDLKQNLTALEKELKDLNNTLHQLRRELENYLTAGLAEQFANVLKYYQRSLNSEQRCNASVYGPQSPVEQSQDTRNRTEALLKDKKDSLLRTATANNKSLSELEGKAHEINRKVHHLSNKVCGGHANGNVNGSCEESPCGGAGCRGSDGVLKCGGRGCNGTVGASVKALDNADEVHKNLTAVSEELQTMAKKLRDIATLTQTVKTQAKETLDKAQNKKDMFEKSNKMLKNFIQKIKDFLNEEGADPESIEKVAQQVLAISLPVNRTVIVNLVEQIKDSIVNLTNVEGVFNHTSEQLAKVNDLLKKAQDAKTQADGVSDNINKTKEALETSQNAIKKAEEEMSTALKNLKNIQNITAMVDDKLLNLDNNLTDVMMRLVNLSNGVDLLKNKTEQNREMAKEAKAQSDNATREAEGLQEELTNAETLYKQLKEKVDSAGGTGEGNVNQKAIEMKKEAEDLLKKATMGMETLKKLERKFNKNEQKMQQQRDELTDLEKNVTGIREYIRSKVLAYNNCQ

Target ORF information:

RefSeq Version	XM_687746.8
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio laminin, beta 2-like (lamb2l), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_687746.8

ORF Insert Sequence:

ATGATGGAGA GATATTTCAG TGTTCTGCTT CTTGTTCTTC CTGCTTTGGC TCAGGATCTG 
CCCTCTGCCC CACATGGGTG CATGGATGGT AGCTGTTATC CAGCAACAGG CAACCTTCTG 
ATTGGCCGAG CTATAAACCT GACCACTTCC TCCACCTGTG GCCTGGATGG ACCCGAGCCA 
TACTGTATTG TCAGTCATTT GAAGGATTCA GAAAAGTGCT TTATATGTGA CTCTGAGAAT 
CCGTATGATG CCAATTATCA CAGAGACAGC CACAGAGTGG AGAACGTCAT CTACCCGATG 
GACAGCACAG GGGAACTCAC ATGGTGGCAG GCTGTAAATG GAGAGGAGAA TGTCAGTGTC 
AGACTGAATC TTGAGGCCGA GTTCCACTTT ACTCATCTCA TCATGAAGTT TAAGACATTT 
AGACCTGCTG CTATGATAAT TGAGCGTTCC TCTGATTTTG GCCGTACATG GAGGATATAC 
CGCTACTTTG CTTACAACTG CACTAAGACA TTTCCTAGGG TTCCTGCGCA CTCGCTGCGG 
TTCATTGATG AAGTCATTTG TGAAGAGCGC TACTCTGATA TTGAGCCATC CACTGATGGA 
GAGGTCATCT ACAAAGTGCT TGATCCTGCA ATCCATGTCA AAGATCCATA TAGTCTGGAC 
ATTCAGGATT TGTTGCGGAT CACTAACCTA CGGATAAACT TCACTAAACT GCACACTCTG 
GGTGATAACC TGCTTGACCG GCGTCCTGAC GTGCTGCAGA AGTATTACTA TGCATTGTAT 
GAGCTGGTTG TGCGCGGGAG CTGCTTCTGT TATGGCCATG CATCAGAGTG TGCACCAGTA 
CCTGGAGTGA CCACTAGAGA TTCAGGCATG ATTCATGGAC GCTGTGTCTG TAAGCACAAC 
ACTGTGGGAT TAAACTGTGA GCGCTGCAGA GATTTTTATC ATGATGCACC CTGGAGGCCT 
GCTGAGACCG ATAATGCACA CACCTGCAGG GAATGCAACT GTAATGGTCA CTCCAACCAG 
TGTCATTTTG ACATGGCGGT GTATTTGGCG ACGGGGAATG TCAGTGGAGG TGTGTGTAAC 
AACTGTCTTC ACAACACCAT GGGCCGCAAC TGTGAGAGCT GTAAGCCTTT CTACTACCAG 
GATCCCGAGA AAGACATCAG AGACCCGGCA GCGTGTGTCC CCTGTGACTG TGACCCCGTG 
GGCTCTGTGG AGGGAGGAGT GTGTGACAGT CATACAGATC TGGATTTGGG AATGATTGGT 
GGTCAGTGCC GTTGTAAGCA GAATGTCAGA GGTCAGCGCT GCGACTACTG TAAAGAGGGA 
CATTATGGTC TCAGTCTTGA TGACCCATTA GGATGCCAGC CTTGTAACTG TGACCCTCGT 
GGCATCACTA CACTGGGGGT TCCGTGTGAT CAGATTAGTG GGGACTGCTC TTGCAAAAGA 
TATGTAACAG GACGCTACTG CAACCAGTGC CTGCCTGAAT ATTGGGGACT CAGTCATGAT 
CTGGCTGGTT GCAGGCCCTG CAACTGTGAC TTTGGTGGAG CAACAAGCAG CAGATGCATG 
ATGGACAGCG GTCAGTGTGA CTGCCGGCCT CATCTGATTG GACGACAGTG TTCTGAAGTG 
CAGCCTGCAT TTTTCTGTGC TTCTTTGGAT TATTACAAGT ATGAAGCAGA AGCAGTTATG 
GGCCGCTCTC CTGAAGATCT CACACTTCCA GGCAGCCCAA GGCCTCAGGC TGAGACTGAC 
TGTGTGCAGC ATCTGAACAA TCAACTGAGA AGACACCGCC GTCACAGACA TATTGCACAA 
CAGCAGAGGG CAGCACTGAG ACGCATCCGA CAGCTGCAGC AAACACCCAG CGTGAGCTCA 
GTGCACAGAG AGAGACAGCC GGGTCAGATG GTCACCTGGA CCGGGCCGGG CTTCGCTCGG 
GTTAAAGACG GGGCTGGTCT TGAGTTTGCC ATCAGCAACA TCCCTTATGC CATGGAGTAT 
GACATTATGA TCCGATATGA GCCAGAGTCC ACAGAAGACT GGGAGGCTTT AGTGAGCGTC 
ACATCCGTGA ATTTACCCAT AAGCCTCCGC TGTGGCAACA TGCTGCCCAC AGAGCAGCTG 
TATACAGTCC CAATGCCCCA CAACGTGAGG TACATTAAAA TGCCAAAGCC CTTCTGCTTC 
GAGCCAAACA ACCGCTATGT GGTGTCCATT CGGTTCCAGC GCCACGCCGT GTCTCACCGC 
CATCTTACCG CCTTCATCCT GATAGACTCA CTTGTGCTGA TTCCCAGATA TGAAGAGCTT 
ACTGGATTTC AGGGTGATGA AGGTGCAGAT GAGCGTCGTC GTCAGGAGAT GGAGTCGTAC 
ATGTGTTTGG AGTCCTTCAT GACTATGCCA ATGCCAGCGC TGGCTGAACT GTGCACTTCT 
CTTATCTGCA GCATCTCTGC TATCATGCAC AATGGTGCAC TGGGGTGTCA GTGTGACCCT 
CAGGGTTCAT TAAGTGCAGA GTGTGACAGA GTCGGAGGTC AGTGTCGCTG TAAGCCCAAC 
GTGATTGGCC GTAAATGTAA TCAGTGTGCA CCGGGGACAT ATGGATTCGG CCCCTACGGC 
TGCACTGTAT GTGACTGTCA CTCGCAGGGT TCTGTCGGGC ATCATTGTGA CCCTGTGACG 
GGACAGTGTA TGTGTCGGCA TGGGGCGATT GGCCGCCAGT GCTCAGAATG CCAGCCGGGT 
CAGTGGGGAT TTCCTAACTG TAGGCAATGC CAGTGTAACA GCCATGCAGA GAGCTGCGAT 
CCTGAAAGCG GGGCCTGCTA TGACTGCAGA GACAACACAG CTGGACAACT GTGCGAGAGG 
TGTGTGGATG GTTATTATGG CAACCCAGCA CTTGGTTCTG GTGAGCACTG TCGCCCATGT 
CCGTGTCCAG GACACCCTGA CAGTGGACAT TCCAACGGTG CCTCCTGTGA AGTAGACTAC 
GCCTCCAACC AGATCCTTTG CCAGTGTGGG CAAGGCTACG CTGGTCCTCG CTGTGATCGC 
TGCGCCCCCG GTTATTATGG TGACCCCGAG CACCCTGGTG GATCGTGCCG GCCCTGCCAG 
TGCAATGGGA ACATTGACCC TCAGGACCCA GAGTCATGTG ACCCTCGAAC TGGACTGTGC 
CTCAAGTGTC TGTATAACAC AGATGGAGAC TCTTGCTCAG AGTGTAAACT GGGTTATTAT 
GGAAATGCAC TCATTCAGGA CTGTAGGCGA TGTACTTGTG TAACTGCAGG CACTTTGCCA 
AACTCTTGCA CTGATGGAGT GTGTCAATGT GATAAACAGA CAGGACAGTG CCCCTGCAAG 
CAAAACATCA TTGGACATAA CTGTGACCAG TGCGCCACCA ATCACTGGAA CTTCGGCTCT 
GACTGTGGAT GTGAACCCTG TGGATGTGTC CAACCTAATG CCCTCAGCAC CCACTGTAAC 
ATGTTTAGTG GCCAGTGTCA CTGTCAGCCA GGTTTTGGGG GGCGGCAGTG CAATGAGTGT 
GAGGCGTTCC ACTGGGGCGA TCCCAGAGTG CAATGTGAAG AGTGTAACTG CCATCCTCTG 
GGCTCTGAGA CGTCTCAGTG TGACCGCACA ACAGGCGCAT GTGTGTGTAA GGACGGAGCT 
TCAGGACAGC GCTGTGATGA GTGTGCACGC GGCTTTACTG GAAACTTCCC CAAATGTGTT 
CCCTGCCACC CCTGCTTCCA GCTGTGGGAT GATGTGGTGT GCCAGGTCCG CCGAGATCTG 
ACCCACTTCA GGGATATCAT TGCTATGATT CTGGAGAAGG GTGAGGTGCC TGGAGTCAGC 
GACAAGCGCA TACTTGAACT AGAGAAGAAG CTGGCACAGG CTCAAGAACT GGTCCGGGAT 
GGAGACAGAG AGGGAACATT TAACTTAATC AGCCAAACTG TTGATGACTT GAGAGCGGAG 
ATCGCTTTGA CTGATGGACG TCTGATGGGT GTAATACGGG ATCTGAATGT GACAACAGAC 
CATAACAATG ATCTGAAACA AAACCTAACT GCCCTGGAGA AAGAACTGAA AGATCTGAAC 
AATACGCTTC ACCAGCTGCG CAGAGAGCTG GAGAACTACC TCACTGCTGG ACTGGCAGAG 
CAATTTGCAA ATGTGCTGAA GTATTACCAG AGATCCCTGA ATTCAGAGCA GCGCTGCAAT 
GCCTCCGTAT ACGGACCCCA GAGTCCTGTG GAACAGTCTC AGGACACACG TAACCGTACC 
GAAGCCCTGC TGAAGGACAA GAAGGACAGC CTCCTGCGTA CAGCCACAGC TAACAATAAA 
TCCCTGTCTG AACTGGAGGG AAAAGCTCAT GAGATCAACC GAAAGGTCCA TCACCTTAGC 
AACAAGGTCT GCGGTGGCCA CGCCAATGGC AATGTGAATG GTAGCTGTGA AGAGAGCCCC 
TGTGGAGGAG CGGGATGTCG TGGCAGTGAT GGTGTGCTGA AGTGTGGAGG TAGAGGATGT 
AATGGAACTG TTGGTGCTTC AGTGAAGGCT TTAGACAATG CTGATGAGGT CCATAAGAAC 
CTGACTGCTG TTAGCGAGGA GCTCCAAACC ATGGCTAAGA AGCTACGAGA CATTGCTACG 
CTCACTCAAA CTGTGAAAAC ACAAGCCAAA GAAACTCTCG ATAAAGCCCA GAACAAGAAG 
GACATGTTTG AGAAGTCCAA CAAGATGCTC AAAAACTTCA TCCAGAAGAT TAAAGACTTT 
CTGAATGAGG AGGGAGCAGA TCCAGAAAGC ATTGAAAAAG TGGCCCAGCA GGTTCTGGCA 
ATCTCCTTGC CAGTCAACAG GACTGTAATA GTCAACTTAG TGGAGCAAAT CAAAGACAGC 
ATCGTCAACC TCACCAACGT GGAGGGAGTG TTCAATCACA CCTCAGAACA ACTGGCCAAA 
GTCAACGATC TACTCAAAAA AGCTCAGGAT GCAAAGACAC AAGCTGATGG AGTGAGTGAT 
AATATCAATA AAACAAAAGA AGCACTTGAA ACCTCACAGA ATGCCATAAA GAAAGCTGAA 
GAGGAAATGA GCACAGCTCT AAAAAACCTC AAGAATATCC AGAACATCAC TGCCATGGTT 
GACGACAAAC TCTTAAATTT AGACAACAAC CTTACAGATG TGATGATGCG TTTAGTCAAT 
TTGTCAAATG GAGTGGATTT ATTAAAGAAT AAAACGGAGC AGAACAGGGA GATGGCCAAA 
GAAGCCAAAG CCCAGTCGGA CAATGCAACT CGAGAAGCAG AGGGACTTCA AGAGGAGCTG 
ACAAATGCTG AGACTCTGTA CAAACAGCTG AAAGAGAAGG TGGACTCGGC TGGAGGGACA 
GGAGAAGGCA ATGTTAACCA GAAAGCCATA GAGATGAAGA AAGAAGCAGA AGATCTGCTG 
AAGAAGGCCA CCATGGGCAT GGAAACTCTC AAAAAATTGG AGAGAAAGTT CAACAAGAAC 
GAGCAGAAAA TGCAGCAGCA ACGGGATGAA CTGACAGATC TGGAGAAAAA TGTTACAGGC 
ATCAGAGAAT ATATCAGATC AAAAGTATTG GCCTACAACA ACTGTCAGTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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lamb2l cDNA ORF clone, Danio rerio(zebrafish)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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