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chrna6 cDNA ORF clone, Danio rerio(zebrafish)

Summary

Gene Symbol chrna6
Entrez Gene ID 555747
Full Name cholinergic receptor, nicotinic, alpha 6
Synonyms si:dkey-234d14.1
General protein information
Preferred Names

cholinergic receptor, nicotinic, alpha 6

Names

neuronal acetylcholine receptor subunit alpha-6
neuronal nicotinic acetylcholine receptor alpha 6
Gene Type protein-coding
Organism Danio rerio(zebrafish)

Reference Sequences (RefSeq)

mRNA and Protein(s)

mRNA Protein Name
NM_001042684.2 NP_001036149.1 neuronal acetylcholine receptor subunit alpha-6 precursor

Pathways from BioSystems


Homologies


Pan troglodytes (chimpanzee) CHRNA6 NP_001029266.1
Drosophila melanogaster (fruit fly) nAcRalpha-7E NP_525079.3
Anopheles gambiae (African malaria mosquito) AgaP_AGAP000329 XP_310786.3
Homo sapiens (human) CHRNA6 NP_004189.1
Bos taurus (cattle) CHRNA6 XP_002698774.2
Gallus gallus (chicken) CHRNA6 NP_990695.1
CHRNA6 XP_001099152.1
Rattus norvegicus (Norway rat) Chrna6 NP_476532.1
Caenorhabditis elegans (roundworm) unc-38 NP_491472.1
Xenopus tropicalis (tropical clawed frog) LOC100495287 XP_002942944.2
Canis lupus familiaris (dog) CHRNA6 XP_539951.2
Mus musculus (house mouse) Chrna6 NP_067344.2
Danio rerio (zebrafish) chrna6 NP_001036149.1

Gene Ontology


Bibliography

Related articles in PubMed

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
Pasquier J, Cabau C, Nguyen T, Jouanno E, Severac D, Braasch I, Journot L, Pontarotti P, Klopp C, Postlethwait JH, Guiguen Y, Bobe J
BMC genomics17368(2016 05)

Apela Regulates Fluid Homeostasis by Binding to the APJ Receptor to Activate Gi Signaling.
Deng C, Chen H, Yang N, Feng Y, Hsueh AJ
The Journal of biological chemistry290(30)18261-8(2015 Jul)

Developmental role of acetylcholinesterase in impulse control in zebrafish.
Parker MO, Brock AJ, Sudwarts A, Teh MT, Combe FJ, Brennan CH
Frontiers in behavioral neuroscience9271(2015)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

and chrna4 RNAs were each expressed in a unique pattern, which changed during development
Title: Cloning and spatiotemporal expression of zebrafish neuronal nicotinic acetylcholine receptor alpha 6 and alpha 4 subunit RNAs.

The following chrna6 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the chrna6 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
ODa35800 NM_001042684.2
Latest version!		 Danio rerio cholinergic receptor, nicotinic, alpha 6 (chrna6), mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $342.30
$489.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)


CloneID ODa35800
Clone ID Related Accession (Same CDS sequence) NM_001042684.2
Accession Version NM_001042684.2 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 1539bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2019-09-14
Organism Danio rerio(zebrafish)
Product neuronal acetylcholine receptor subunit alpha-6 precursor
Comment Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from DQ822507.1. On Aug 31, 2012 this sequence version replaced NM_001042684.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: DQ822507.1, BC171403.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA3505370, SAMEA3505371 [ECO:0000350] ##Evidence-Data-END## 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
ATGAATTCTG CGGGGAGAGT CACTTTGTTT TTTCTTTTAA TTACCCTGAT AACGCAAGAT 
TGTTTCTCGT CTAAAGGAGA AGACAGGCTC TTTCGTCGTT TATTCAGGAG GTATAATCAG 
TTCATTCGGC CAGTGGAAAA TGTGTCTGAC CCTGTTACTG TGGAGTTTGA GGTCTCCATC 
TCACAGCTTG TTAAAGTGGA TGAAGTCAAT CAGATCATGG AAACAAACCT TTGGCTGAGA 
CATATATGGA ATGACTACAA GCTGAAGTGG TCACCAGCAG AGTTTGATGG AATTGAGTTC 
ATCAGAGTCC CATCCAATAA AATCTGGAGG CCTGACATTG TACTTTACAA CAATGCTGTT 
GGGGACTTCC TGGTGGAGGA CAAAACCAAA GCCCTTTTGA AATTTGATGG AACCATCACT 
TGGGTCCCAC CTGCAATTTT CAAATCTTCC TGCCCTATGG ACATCACTTA CTTTCCTTTT 
GACTATCAGA ACTGCTCTAT GAAGTTTGGT TCCTGGACGT ATGATAAGGC CAAAATTGAC 
CTGGTTCTTA TCGGCTCTAA GGTCAACCTT AAAGACTTCT GGGAAAGTGG AGAGTGGGAG 
ATCATTGATG CACCGGGTTA TAAGCATGAC ATCAAGTACA ACTGCTGTGA AGAGATCTAC 
CCAGATATAA CCTACAGTTT TTATATACGC CGACTACCCC TCTTCTACAC TATCAACCTT 
ATAATCCCCT GCCTCCTCAT CTCTTTTCTG ACAGTACTGG TTTTCTACCT TCCATCAGAC 
TGTGGCGAGA AGGTGACCTT GTGCATCTCT GTACTTCTAT CTCTCACTGT GTTCCTTCTT 
GTCATTACGG AAACCATCCC ATCTACTTCA CTTGTGATCC CTCTGATTGG CGAGTACCTT 
CTCTTCACCA TGATCTTCGT AACCCTCTCT ATTGTTATCA CTGTCTTTGT ACTGAATGTG 
CACTATCGCA CCCCTATGAC CCACACTATG CCCAGCTGGG TCCACACTGT CTTCCTTCGA 
GCTTTACCCC GCATCATGCT CATGCGCAGG CCTCTTGATC TATCAGAGTC CTCTGGAAAA 
GGAGGACTAG AAACTGGGGG CTCGTCCGGG ACAGGAGCAG GGCGCGGAGG AGAGGGGAAG 
AAGAGAAAGA ATAGTGGATC CCAACAGGGT GCAATGAACT CTCTGGAATT TGGAGAGGGG 
AAAGCAGCAT TGGAAGGAAA GAAAGGAGGA TGCCCATGTC ATCCACTGAA AGAAGCAACT 
GAGGGAGATT GTGGGAAAGT CAGCCGTCAA TTAAGTCCAC AGGCTATTAA TACAGTTGTG 
GCTTTCTCTG TCGTGTCACC GGAGATCAAA CAGGCTATTG AAAGTGTGAA GTACATTGCT 
GAGAACATGA GGAGCCGCAA CAAAGCTAAA GAGGTGGAGG ATGATTGGAA GTATGTTGCC 
ATGGTGATTG ACCGCATCTT CCTCTGGGTC TTTGTGCTGG TGTGTGTGTT AGGAACTTTG 
GGCCTCTTCC TGCAACCTTT GATTGGCTTC CTCTCGTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001036149.1
CDS159..1697
Translation

Target ORF information:

RefSeq Version NM_001042684.2
Organism Danio rerio(zebrafish)
Definition Danio rerio cholinergic receptor, nicotinic, alpha 6 (chrna6), mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 NM_001042684.2

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
ATGAATTCTG CGGGGAGAGT CACTTTGTTT TTTCTTTTAA TTACCCTGAT AACGCAAGAT 
TGTTTCTCGT CTAAAGGAGA AGACAGGCTC TTTCGTCGTT TATTCAGGAG GTATAATCAG 
TTCATTCGGC CAGTGGAAAA TGTGTCTGAC CCTGTTACTG TGGAGTTTGA GGTCTCCATC 
TCACAGCTTG TTAAAGTGGA TGAAGTCAAT CAGATCATGG AAACAAACCT TTGGCTGAGA 
CATATATGGA ATGACTACAA GCTGAAGTGG TCACCAGCAG AGTTTGATGG AATTGAGTTC 
ATCAGAGTCC CATCCAATAA AATCTGGAGG CCTGACATTG TACTTTACAA CAATGCTGTT 
GGGGACTTCC TGGTGGAGGA CAAAACCAAA GCCCTTTTGA AATTTGATGG AACCATCACT 
TGGGTCCCAC CTGCAATTTT CAAATCTTCC TGCCCTATGG ACATCACTTA CTTTCCTTTT 
GACTATCAGA ACTGCTCTAT GAAGTTTGGT TCCTGGACGT ATGATAAGGC CAAAATTGAC 
CTGGTTCTTA TCGGCTCTAA GGTCAACCTT AAAGACTTCT GGGAAAGTGG AGAGTGGGAG 
ATCATTGATG CACCGGGTTA TAAGCATGAC ATCAAGTACA ACTGCTGTGA AGAGATCTAC 
CCAGATATAA CCTACAGTTT TTATATACGC CGACTACCCC TCTTCTACAC TATCAACCTT 
ATAATCCCCT GCCTCCTCAT CTCTTTTCTG ACAGTACTGG TTTTCTACCT TCCATCAGAC 
TGTGGCGAGA AGGTGACCTT GTGCATCTCT GTACTTCTAT CTCTCACTGT GTTCCTTCTT 
GTCATTACGG AAACCATCCC ATCTACTTCA CTTGTGATCC CTCTGATTGG CGAGTACCTT 
CTCTTCACCA TGATCTTCGT AACCCTCTCT ATTGTTATCA CTGTCTTTGT ACTGAATGTG 
CACTATCGCA CCCCTATGAC CCACACTATG CCCAGCTGGG TCCACACTGT CTTCCTTCGA 
GCTTTACCCC GCATCATGCT CATGCGCAGG CCTCTTGATC TATCAGAGTC CTCTGGAAAA 
GGAGGACTAG AAACTGGGGG CTCGTCCGGG ACAGGAGCAG GGCGCGGAGG AGAGGGGAAG 
AAGAGAAAGA ATAGTGGATC CCAACAGGGT GCAATGAACT CTCTGGAATT TGGAGAGGGG 
AAAGCAGCAT TGGAAGGAAA GAAAGGAGGA TGCCCATGTC ATCCACTGAA AGAAGCAACT 
GAGGGAGATT GTGGGAAAGT CAGCCGTCAA TTAAGTCCAC AGGCTATTAA TACAGTTGTG 
GCTTTCTCTG TCGTGTCACC GGAGATCAAA CAGGCTATTG AAAGTGTGAA GTACATTGCT 
GAGAACATGA GGAGCCGCAA CAAAGCTAAA GAGGTGGAGG ATGATTGGAA GTATGTTGCC 
ATGGTGATTG ACCGCATCTT CCTCTGGGTC TTTGTGCTGG TGTGTGTGTT AGGAACTTTG 
GGCCTCTTCC TGCAACCTTT GATTGGCTTC CTCTCGTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

PubMed

book

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
BMC genomics17368(2016 05)
Pasquier J,Cabau C,Nguyen T,Jouanno E,Severac D,Braasch I,Journot L,Pontarotti P,Klopp C,Postlethwait JH,Guiguen Y,Bobe J


book

Apela Regulates Fluid Homeostasis by Binding to the APJ Receptor to Activate Gi Signaling.
The Journal of biological chemistry290(30)18261-8(2015 Jul)
Deng C,Chen H,Yang N,Feng Y,Hsueh AJ


book

Developmental role of acetylcholinesterase in impulse control in zebrafish.
Frontiers in behavioral neuroscience9271(2015)
Parker MO,Brock AJ,Sudwarts A,Teh MT,Combe FJ,Brennan CH