AFF4 cDNA ORF clone, Danio rerio(Zebrafish)

The following AFF4 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the AFF4 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
ODa26232 XM_005173899.4
Latest version!
Danio rerio AF4/FMR2 family, member 4 (aff4), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $629.30
$899.00
ODa26233 XM_005173900.4
Latest version!
Danio rerio AF4/FMR2 family, member 4 (aff4), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $629.30
$899.00
ODa26234 XM_679235.10
Latest version!
Danio rerio AF4/FMR2 family, member 4 (aff4), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $629.30
$899.00
View Old Accession Versions >>
ODa26234 XM_679235.9 Danio rerio AF4/FMR2 family, member 4 (aff4), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $629.30
$899.00
ODa26232 XM_005173899.3 Danio rerio AF4/FMR2 family, member 4 (aff4), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $629.30
$899.00
ODa26233 XM_005173900.3 Danio rerio AF4/FMR2 family, member 4 (aff4), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $629.30
$899.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID ODa26232
    Clone ID Related Accession (Same CDS sequence) XM_005173899.3 , XM_005173899.4
    Accession Version XM_005173899.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3258bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product AF4/FMR2 family member 4 isoform X1
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007132.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005173899.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    ATGGCCTCTC AGTCAGGCAA CATGAACCGT GAAGACCGGA ATGTGCTCCG TATGAAAGAA 
    AGGGAAAGGA GAAATCAAGA AATCCAGCAG GGAGGAGAGG CCTTTCCAGC TAACTCCCCC
    CTCTTTCCTG AACCTATTAA AGTTGCCAAG GAAGATAAAC TCTCAAGTCG CATCCAGAGC
    ATGCTGGGAA ATTACGACGA GATGAAGGAA ACCATTGGTG AACCGCCAAT GTCCAAGCTC
    ATTTCCAAAG CTTCCAGCTC TTCCTCCGAG GATAAATCTG GCCAGTATGG TGACCAGCGC
    GGAGGCTCTC AGACTCAAAG CAGCAAGTGG ACCCCGGTGG GTCCGGCCTC AGGAGGTGGC
    AACTCTTCAT CGTCTTCTCA GAAACGCTCA ACTGTGCAGA GCGTCAGCCA GAAGAGCAGC
    TCCAGCGGGC AGCGGCACGA GCGGGACTAC AGCGGCAGCA AGAAATCCAG CAAGCACAGC
    AACGAGCACA AGTCCCACTC CAGTTCCAGC CCGGCCAAAG TCTCCAGCAA CCACTCGCGC
    CGAGTCCCCA AGTCCCCCAG AGACAGAGAG GCCAATTGGG ACTCGCCCTC CATCCCGCAT
    TCCTTCCCCA GTGGACATCC CAACCAAACC TTCCCACCCT CGCTCATCAA ATCCAGCTCC
    ATGCCCCAGA AGCCCACCGC TTACGTTCGG CCCATGGACG GCCAGGAGAC GACCGAGGCA
    AAGACGTCTT CAGATTCGTA CAGCGGCCAA TCGCACAGCA GCACAGTTGG AGAAATGAAG
    CCCAATGGAA AGGGCCCACT CCCCAAGCTC AAGATCCCAA CACAGCCTCC AGAGGCGGGG
    GATGCGAGCT GTGTTGATGA GATTTTAAAG GAAATGACTC AGGTCTGGCC TCCTCCATTA
    ACAGCCATTC ACACCCCCTG CAAGACCGAG CCGCCTTCCA AATTCCCCTT CTCCAACGCT
    AAGGATGCCC AGCACCAGTC TGTTGGGGCT CAAAAAAAGT TCTCAGGGAA GAGCGCATCA
    ACCAGTCACC CGTCAAAGCC ATGTGACAGC GAGCAGTCCA ACATGCTTCA TGATGATCTT
    AAGCTCAGCA GCAGTGAAGA CAGTGACGGA GAGCAAGATC CTGCCAAAAA CACCCCTCAA
    AACAACTCCA CAAGTAACAA CAGTAATAAC AGTGAGGGAG CGGAGCGAGA GGACACCAGC
    AGTCACAGCG GCTCCGAGAG CAGCTCCGGG TCCGACAGCG AGAGCGAGAG CAGCTCCACC
    GACAGCGAGA CCACCGAACC GCCACGAGCT GCGACCCCCG AGCGGGAACA GCCTACGGCT
    AACAAATGGC TTCTGGACAA CTGGTTAAAT AAGGTCAACA AGCTGTCTCC TGCTTCTCCG
    GTGGAAAACA GCAGCAGCAG CACGCCGGGA ACCAAGTATA AGAAGGAGGG TCGAGATACC
    AGCTCCAGCA GGAGTTACAG TGGGCAGGGG TCTAAAGAAG CTGCAACGTC AGCCGGCCGA
    GAGTTGCGAC CCAATCAGAA GAGCTCCGAA AGCTGGCGAG GACGCAGTCA GAAATCTCCT
    GCTCAGAGCG AGGGCACAAG TCAATCCGGG CGACGGGTCG TAGGCAAAAA ACAGCCCAAA
    AAACCTGAGA AGCCGCCGGT GGTTGAAGAG CCCAAGGGTG GACTAAAAGT AGAAACCGAA
    CCATCGCCGG AAATCCCGTC ACACAGAGCT CGCGCTCCCA ATAAAGGCCT GCGCAAACCC
    AACATTAAAA AAGAACCCAA AACCTCCTCT CCACGACCTC CGCCTGATAA GCGCAAGACG
    AAAGCGGCAA AAGTGCCCAG CAAGTCTCGC GAGTTCATCG AATCGGATTC GTCGACGTCG
    GATTCTGAAG GCAACGAAAG CGACCCATTG CCTTCTCAGA CGCCGAAAGA TTACAGCGAA
    AACCCGTTGG TGCCACTCTC GCCCATTTAT GACCCGCCCA GACTGCTGGT GCAGATCAAT
    CTCAGCCTGC TGACTAGAGT CCCGGGACAG CTCTACAATG AGACGGAGGT TAAGGTGGAG
    CGAGGGTCTC CAGCGGAGGG AAAAAGCAAC CAGAAGCAGC CCGGAGAAAA GATAAGCAAG
    GAAGGAAGGA AGAGGAAACA CAAGAATGAA GATGATGGTG TGAAACCAGA GAGTAAGAAG
    TCCAAACTGG ATGAGAAGTC TTCACGCAAA TCAAGCAGTA ATGATTCTTC CAAGCGTGCT
    CAGGAAAAAG AGAAGGAGTC TGCGCCGTCT CCCTCCATCA CACTGCAGCG GACGCCCAAA
    TCTGAGCAGC AGGGGCGCAA GCGGACCCTC AGCCAGTCCT CCAGCACCGT CCCCAGCAAA
    GACAAGAGCA GCTCCAGCTC CAGACACCGC AGAGGAGACG ACAAACCCCA GCGCAGAGAG
    CCCAAGGAGA AAAGCTCCAA ATCAGAGAAT CCCGTTTCAG TGCCGCCGCT CTCTGAGGGC
    TCCAAATCTA GATCTAAACT TCTGTTTGAG GACAGGGTTC ATTCGGCTGA TCATTACCTT
    CAAGAAGCCA AGAAGTTGAA ACACAATGCG GATGCTCTGG TGAGGATGGA CAGATTTGAG
    AAGGCGGTGT ATTACCTGGA CGCCGTGGTG TCTTTCATCG AGTGTGGAAA TGCCCTGGAG
    AAAAACGCCC AGGAGGCCAA ATCCCCCTTT CCCATGTATG CAGAAACCGT GGAGCTCATC
    AAGTACACTA TGAAGCTAAA GAGCTACATG GCACCAGATG CAACTTCTCA AGACAAAAGG
    CTAGCAGTGC TATGTCTTCG ATGTCAAGCG CTCCTGTATT TGAGGCTTTT CAAGCTGCGG
    AAAGAAAGTG CACTGAAATA CTCTAAAACA CTCACTGAAC ACTTGAAGAA TTCATTGAGT
    AATACCCAAG CTCCGTCTCC TGGAGTAGCA AATAAAAGCG CAGGGATGCC GTCTCCGGTC
    TCCCCCAAAC TCTCTCCTGG CAGTGCGGGC AGCTACTCCT CCAGCAGCTC CAGCCAAAGC
    GCCAGCTCCT CCGTCACCAT CCCGCAGCGC ATCCACCAGA TGGCGGCAAG CTACGTGCAA
    GTCACCTCCA ACTTCCTGTA CGCCACCGAG GTGTGGGACC AGGCTGAGCA GCTCGCCAAA
    GAGCAGAGAG AGTTCTTCAC TGAGCTGGAC AAGGCGATGG GTCCGCTGAT CTTCAACACC
    AGCAGCATGA CTGACCTGGT GCGGTTCACG CGGCAGGGCC TGCACTGGCT ACGACTGGAC
    GCCAAGCTCA TCCAGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005173956.1
    CDS528..3785
    Translation

    Target ORF information:

    RefSeq Version XM_005173899.3
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio AF4/FMR2 family, member 4 (aff4), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005173899.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    ATGGCCTCTC AGTCAGGCAA CATGAACCGT GAAGACCGGA ATGTGCTCCG TATGAAAGAA 
    AGGGAAAGGA GAAATCAAGA AATCCAGCAG GGAGGAGAGG CCTTTCCAGC TAACTCCCCC
    CTCTTTCCTG AACCTATTAA AGTTGCCAAG GAAGATAAAC TCTCAAGTCG CATCCAGAGC
    ATGCTGGGAA ATTACGACGA GATGAAGGAA ACCATTGGTG AACCGCCAAT GTCCAAGCTC
    ATTTCCAAAG CTTCCAGCTC TTCCTCCGAG GATAAATCTG GCCAGTATGG TGACCAGCGC
    GGAGGCTCTC AGACTCAAAG CAGCAAGTGG ACCCCGGTGG GTCCGGCCTC AGGAGGTGGC
    AACTCTTCAT CGTCTTCTCA GAAACGCTCA ACTGTGCAGA GCGTCAGCCA GAAGAGCAGC
    TCCAGCGGGC AGCGGCACGA GCGGGACTAC AGCGGCAGCA AGAAATCCAG CAAGCACAGC
    AACGAGCACA AGTCCCACTC CAGTTCCAGC CCGGCCAAAG TCTCCAGCAA CCACTCGCGC
    CGAGTCCCCA AGTCCCCCAG AGACAGAGAG GCCAATTGGG ACTCGCCCTC CATCCCGCAT
    TCCTTCCCCA GTGGACATCC CAACCAAACC TTCCCACCCT CGCTCATCAA ATCCAGCTCC
    ATGCCCCAGA AGCCCACCGC TTACGTTCGG CCCATGGACG GCCAGGAGAC GACCGAGGCA
    AAGACGTCTT CAGATTCGTA CAGCGGCCAA TCGCACAGCA GCACAGTTGG AGAAATGAAG
    CCCAATGGAA AGGGCCCACT CCCCAAGCTC AAGATCCCAA CACAGCCTCC AGAGGCGGGG
    GATGCGAGCT GTGTTGATGA GATTTTAAAG GAAATGACTC AGGTCTGGCC TCCTCCATTA
    ACAGCCATTC ACACCCCCTG CAAGACCGAG CCGCCTTCCA AATTCCCCTT CTCCAACGCT
    AAGGATGCCC AGCACCAGTC TGTTGGGGCT CAAAAAAAGT TCTCAGGGAA GAGCGCATCA
    ACCAGTCACC CGTCAAAGCC ATGTGACAGC GAGCAGTCCA ACATGCTTCA TGATGATCTT
    AAGCTCAGCA GCAGTGAAGA CAGTGACGGA GAGCAAGATC CTGCCAAAAA CACCCCTCAA
    AACAACTCCA CAAGTAACAA CAGTAATAAC AGTGAGGGAG CGGAGCGAGA GGACACCAGC
    AGTCACAGCG GCTCCGAGAG CAGCTCCGGG TCCGACAGCG AGAGCGAGAG CAGCTCCACC
    GACAGCGAGA CCACCGAACC GCCACGAGCT GCGACCCCCG AGCGGGAACA GCCTACGGCT
    AACAAATGGC TTCTGGACAA CTGGTTAAAT AAGGTCAACA AGCTGTCTCC TGCTTCTCCG
    GTGGAAAACA GCAGCAGCAG CACGCCGGGA ACCAAGTATA AGAAGGAGGG TCGAGATACC
    AGCTCCAGCA GGAGTTACAG TGGGCAGGGG TCTAAAGAAG CTGCAACGTC AGCCGGCCGA
    GAGTTGCGAC CCAATCAGAA GAGCTCCGAA AGCTGGCGAG GACGCAGTCA GAAATCTCCT
    GCTCAGAGCG AGGGCACAAG TCAATCCGGG CGACGGGTCG TAGGCAAAAA ACAGCCCAAA
    AAACCTGAGA AGCCGCCGGT GGTTGAAGAG CCCAAGGGTG GACTAAAAGT AGAAACCGAA
    CCATCGCCGG AAATCCCGTC ACACAGAGCT CGCGCTCCCA ATAAAGGCCT GCGCAAACCC
    AACATTAAAA AAGAACCCAA AACCTCCTCT CCACGACCTC CGCCTGATAA GCGCAAGACG
    AAAGCGGCAA AAGTGCCCAG CAAGTCTCGC GAGTTCATCG AATCGGATTC GTCGACGTCG
    GATTCTGAAG GCAACGAAAG CGACCCATTG CCTTCTCAGA CGCCGAAAGA TTACAGCGAA
    AACCCGTTGG TGCCACTCTC GCCCATTTAT GACCCGCCCA GACTGCTGGT GCAGATCAAT
    CTCAGCCTGC TGACTAGAGT CCCGGGACAG CTCTACAATG AGACGGAGGT TAAGGTGGAG
    CGAGGGTCTC CAGCGGAGGG AAAAAGCAAC CAGAAGCAGC CCGGAGAAAA GATAAGCAAG
    GAAGGAAGGA AGAGGAAACA CAAGAATGAA GATGATGGTG TGAAACCAGA GAGTAAGAAG
    TCCAAACTGG ATGAGAAGTC TTCACGCAAA TCAAGCAGTA ATGATTCTTC CAAGCGTGCT
    CAGGAAAAAG AGAAGGAGTC TGCGCCGTCT CCCTCCATCA CACTGCAGCG GACGCCCAAA
    TCTGAGCAGC AGGGGCGCAA GCGGACCCTC AGCCAGTCCT CCAGCACCGT CCCCAGCAAA
    GACAAGAGCA GCTCCAGCTC CAGACACCGC AGAGGAGACG ACAAACCCCA GCGCAGAGAG
    CCCAAGGAGA AAAGCTCCAA ATCAGAGAAT CCCGTTTCAG TGCCGCCGCT CTCTGAGGGC
    TCCAAATCTA GATCTAAACT TCTGTTTGAG GACAGGGTTC ATTCGGCTGA TCATTACCTT
    CAAGAAGCCA AGAAGTTGAA ACACAATGCG GATGCTCTGG TGAGGATGGA CAGATTTGAG
    AAGGCGGTGT ATTACCTGGA CGCCGTGGTG TCTTTCATCG AGTGTGGAAA TGCCCTGGAG
    AAAAACGCCC AGGAGGCCAA ATCCCCCTTT CCCATGTATG CAGAAACCGT GGAGCTCATC
    AAGTACACTA TGAAGCTAAA GAGCTACATG GCACCAGATG CAACTTCTCA AGACAAAAGG
    CTAGCAGTGC TATGTCTTCG ATGTCAAGCG CTCCTGTATT TGAGGCTTTT CAAGCTGCGG
    AAAGAAAGTG CACTGAAATA CTCTAAAACA CTCACTGAAC ACTTGAAGAA TTCATTGAGT
    AATACCCAAG CTCCGTCTCC TGGAGTAGCA AATAAAAGCG CAGGGATGCC GTCTCCGGTC
    TCCCCCAAAC TCTCTCCTGG CAGTGCGGGC AGCTACTCCT CCAGCAGCTC CAGCCAAAGC
    GCCAGCTCCT CCGTCACCAT CCCGCAGCGC ATCCACCAGA TGGCGGCAAG CTACGTGCAA
    GTCACCTCCA ACTTCCTGTA CGCCACCGAG GTGTGGGACC AGGCTGAGCA GCTCGCCAAA
    GAGCAGAGAG AGTTCTTCAC TGAGCTGGAC AAGGCGATGG GTCCGCTGAT CTTCAACACC
    AGCAGCATGA CTGACCTGGT GCGGTTCACG CGGCAGGGCC TGCACTGGCT ACGACTGGAC
    GCCAAGCTCA TCCAGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa26232
    Clone ID Related Accession (Same CDS sequence) XM_005173899.3 , XM_005173899.4
    Accession Version XM_005173899.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3258bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product AF4/FMR2 family member 4 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007132.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005173899.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    ATGGCCTCTC AGTCAGGCAA CATGAACCGT GAAGACCGGA ATGTGCTCCG TATGAAAGAA 
    AGGGAAAGGA GAAATCAAGA AATCCAGCAG GGAGGAGAGG CCTTTCCAGC TAACTCCCCC
    CTCTTTCCTG AACCTATTAA AGTTGCCAAG GAAGATAAAC TCTCAAGTCG CATCCAGAGC
    ATGCTGGGAA ATTACGACGA GATGAAGGAA ACCATTGGTG AACCGCCAAT GTCCAAGCTC
    ATTTCCAAAG CTTCCAGCTC TTCCTCCGAG GATAAATCTG GCCAGTATGG TGACCAGCGC
    GGAGGCTCTC AGACTCAAAG CAGCAAGTGG ACCCCGGTGG GTCCGGCCTC AGGAGGTGGC
    AACTCTTCAT CGTCTTCTCA GAAACGCTCA ACTGTGCAGA GCGTCAGCCA GAAGAGCAGC
    TCCAGCGGGC AGCGGCACGA GCGGGACTAC AGCGGCAGCA AGAAATCCAG CAAGCACAGC
    AACGAGCACA AGTCCCACTC CAGTTCCAGC CCGGCCAAAG TCTCCAGCAA CCACTCGCGC
    CGAGTCCCCA AGTCCCCCAG AGACAGAGAG GCCAATTGGG ACTCGCCCTC CATCCCGCAT
    TCCTTCCCCA GTGGACATCC CAACCAAACC TTCCCACCCT CGCTCATCAA ATCCAGCTCC
    ATGCCCCAGA AGCCCACCGC TTACGTTCGG CCCATGGACG GCCAGGAGAC GACCGAGGCA
    AAGACGTCTT CAGATTCGTA CAGCGGCCAA TCGCACAGCA GCACAGTTGG AGAAATGAAG
    CCCAATGGAA AGGGCCCACT CCCCAAGCTC AAGATCCCAA CACAGCCTCC AGAGGCGGGG
    GATGCGAGCT GTGTTGATGA GATTTTAAAG GAAATGACTC AGGTCTGGCC TCCTCCATTA
    ACAGCCATTC ACACCCCCTG CAAGACCGAG CCGCCTTCCA AATTCCCCTT CTCCAACGCT
    AAGGATGCCC AGCACCAGTC TGTTGGGGCT CAAAAAAAGT TCTCAGGGAA GAGCGCATCA
    ACCAGTCACC CGTCAAAGCC ATGTGACAGC GAGCAGTCCA ACATGCTTCA TGATGATCTT
    AAGCTCAGCA GCAGTGAAGA CAGTGACGGA GAGCAAGATC CTGCCAAAAA CACCCCTCAA
    AACAACTCCA CAAGTAACAA CAGTAATAAC AGTGAGGGAG CGGAGCGAGA GGACACCAGC
    AGTCACAGCG GCTCCGAGAG CAGCTCCGGG TCCGACAGCG AGAGCGAGAG CAGCTCCACC
    GACAGCGAGA CCACCGAACC GCCACGAGCT GCGACCCCCG AGCGGGAACA GCCTACGGCT
    AACAAATGGC TTCTGGACAA CTGGTTAAAT AAGGTCAACA AGCTGTCTCC TGCTTCTCCG
    GTGGAAAACA GCAGCAGCAG CACGCCGGGA ACCAAGTATA AGAAGGAGGG TCGAGATACC
    AGCTCCAGCA GGAGTTACAG TGGGCAGGGG TCTAAAGAAG CTGCAACGTC AGCCGGCCGA
    GAGTTGCGAC CCAATCAGAA GAGCTCCGAA AGCTGGCGAG GACGCAGTCA GAAATCTCCT
    GCTCAGAGCG AGGGCACAAG TCAATCCGGG CGACGGGTCG TAGGCAAAAA ACAGCCCAAA
    AAACCTGAGA AGCCGCCGGT GGTTGAAGAG CCCAAGGGTG GACTAAAAGT AGAAACCGAA
    CCATCGCCGG AAATCCCGTC ACACAGAGCT CGCGCTCCCA ATAAAGGCCT GCGCAAACCC
    AACATTAAAA AAGAACCCAA AACCTCCTCT CCACGACCTC CGCCTGATAA GCGCAAGACG
    AAAGCGGCAA AAGTGCCCAG CAAGTCTCGC GAGTTCATCG AATCGGATTC GTCGACGTCG
    GATTCTGAAG GCAACGAAAG CGACCCATTG CCTTCTCAGA CGCCGAAAGA TTACAGCGAA
    AACCCGTTGG TGCCACTCTC GCCCATTTAT GACCCGCCCA GACTGCTGGT GCAGATCAAT
    CTCAGCCTGC TGACTAGAGT CCCGGGACAG CTCTACAATG AGACGGAGGT TAAGGTGGAG
    CGAGGGTCTC CAGCGGAGGG AAAAAGCAAC CAGAAGCAGC CCGGAGAAAA GATAAGCAAG
    GAAGGAAGGA AGAGGAAACA CAAGAATGAA GATGATGGTG TGAAACCAGA GAGTAAGAAG
    TCCAAACTGG ATGAGAAGTC TTCACGCAAA TCAAGCAGTA ATGATTCTTC CAAGCGTGCT
    CAGGAAAAAG AGAAGGAGTC TGCGCCGTCT CCCTCCATCA CACTGCAGCG GACGCCCAAA
    TCTGAGCAGC AGGGGCGCAA GCGGACCCTC AGCCAGTCCT CCAGCACCGT CCCCAGCAAA
    GACAAGAGCA GCTCCAGCTC CAGACACCGC AGAGGAGACG ACAAACCCCA GCGCAGAGAG
    CCCAAGGAGA AAAGCTCCAA ATCAGAGAAT CCCGTTTCAG TGCCGCCGCT CTCTGAGGGC
    TCCAAATCTA GATCTAAACT TCTGTTTGAG GACAGGGTTC ATTCGGCTGA TCATTACCTT
    CAAGAAGCCA AGAAGTTGAA ACACAATGCG GATGCTCTGG TGAGGATGGA CAGATTTGAG
    AAGGCGGTGT ATTACCTGGA CGCCGTGGTG TCTTTCATCG AGTGTGGAAA TGCCCTGGAG
    AAAAACGCCC AGGAGGCCAA ATCCCCCTTT CCCATGTATG CAGAAACCGT GGAGCTCATC
    AAGTACACTA TGAAGCTAAA GAGCTACATG GCACCAGATG CAACTTCTCA AGACAAAAGG
    CTAGCAGTGC TATGTCTTCG ATGTCAAGCG CTCCTGTATT TGAGGCTTTT CAAGCTGCGG
    AAAGAAAGTG CACTGAAATA CTCTAAAACA CTCACTGAAC ACTTGAAGAA TTCATTGAGT
    AATACCCAAG CTCCGTCTCC TGGAGTAGCA AATAAAAGCG CAGGGATGCC GTCTCCGGTC
    TCCCCCAAAC TCTCTCCTGG CAGTGCGGGC AGCTACTCCT CCAGCAGCTC CAGCCAAAGC
    GCCAGCTCCT CCGTCACCAT CCCGCAGCGC ATCCACCAGA TGGCGGCAAG CTACGTGCAA
    GTCACCTCCA ACTTCCTGTA CGCCACCGAG GTGTGGGACC AGGCTGAGCA GCTCGCCAAA
    GAGCAGAGAG AGTTCTTCAC TGAGCTGGAC AAGGCGATGG GTCCGCTGAT CTTCAACACC
    AGCAGCATGA CTGACCTGGT GCGGTTCACG CGGCAGGGCC TGCACTGGCT ACGACTGGAC
    GCCAAGCTCA TCCAGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005173956.1
    CDS476..3733
    Translation

    Target ORF information:

    RefSeq Version XM_005173899.4
    Organism Danio rerio(zebrafish)
    Definition Danio rerio AF4/FMR2 family, member 4 (aff4), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005173899.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    ATGGCCTCTC AGTCAGGCAA CATGAACCGT GAAGACCGGA ATGTGCTCCG TATGAAAGAA 
    AGGGAAAGGA GAAATCAAGA AATCCAGCAG GGAGGAGAGG CCTTTCCAGC TAACTCCCCC
    CTCTTTCCTG AACCTATTAA AGTTGCCAAG GAAGATAAAC TCTCAAGTCG CATCCAGAGC
    ATGCTGGGAA ATTACGACGA GATGAAGGAA ACCATTGGTG AACCGCCAAT GTCCAAGCTC
    ATTTCCAAAG CTTCCAGCTC TTCCTCCGAG GATAAATCTG GCCAGTATGG TGACCAGCGC
    GGAGGCTCTC AGACTCAAAG CAGCAAGTGG ACCCCGGTGG GTCCGGCCTC AGGAGGTGGC
    AACTCTTCAT CGTCTTCTCA GAAACGCTCA ACTGTGCAGA GCGTCAGCCA GAAGAGCAGC
    TCCAGCGGGC AGCGGCACGA GCGGGACTAC AGCGGCAGCA AGAAATCCAG CAAGCACAGC
    AACGAGCACA AGTCCCACTC CAGTTCCAGC CCGGCCAAAG TCTCCAGCAA CCACTCGCGC
    CGAGTCCCCA AGTCCCCCAG AGACAGAGAG GCCAATTGGG ACTCGCCCTC CATCCCGCAT
    TCCTTCCCCA GTGGACATCC CAACCAAACC TTCCCACCCT CGCTCATCAA ATCCAGCTCC
    ATGCCCCAGA AGCCCACCGC TTACGTTCGG CCCATGGACG GCCAGGAGAC GACCGAGGCA
    AAGACGTCTT CAGATTCGTA CAGCGGCCAA TCGCACAGCA GCACAGTTGG AGAAATGAAG
    CCCAATGGAA AGGGCCCACT CCCCAAGCTC AAGATCCCAA CACAGCCTCC AGAGGCGGGG
    GATGCGAGCT GTGTTGATGA GATTTTAAAG GAAATGACTC AGGTCTGGCC TCCTCCATTA
    ACAGCCATTC ACACCCCCTG CAAGACCGAG CCGCCTTCCA AATTCCCCTT CTCCAACGCT
    AAGGATGCCC AGCACCAGTC TGTTGGGGCT CAAAAAAAGT TCTCAGGGAA GAGCGCATCA
    ACCAGTCACC CGTCAAAGCC ATGTGACAGC GAGCAGTCCA ACATGCTTCA TGATGATCTT
    AAGCTCAGCA GCAGTGAAGA CAGTGACGGA GAGCAAGATC CTGCCAAAAA CACCCCTCAA
    AACAACTCCA CAAGTAACAA CAGTAATAAC AGTGAGGGAG CGGAGCGAGA GGACACCAGC
    AGTCACAGCG GCTCCGAGAG CAGCTCCGGG TCCGACAGCG AGAGCGAGAG CAGCTCCACC
    GACAGCGAGA CCACCGAACC GCCACGAGCT GCGACCCCCG AGCGGGAACA GCCTACGGCT
    AACAAATGGC TTCTGGACAA CTGGTTAAAT AAGGTCAACA AGCTGTCTCC TGCTTCTCCG
    GTGGAAAACA GCAGCAGCAG CACGCCGGGA ACCAAGTATA AGAAGGAGGG TCGAGATACC
    AGCTCCAGCA GGAGTTACAG TGGGCAGGGG TCTAAAGAAG CTGCAACGTC AGCCGGCCGA
    GAGTTGCGAC CCAATCAGAA GAGCTCCGAA AGCTGGCGAG GACGCAGTCA GAAATCTCCT
    GCTCAGAGCG AGGGCACAAG TCAATCCGGG CGACGGGTCG TAGGCAAAAA ACAGCCCAAA
    AAACCTGAGA AGCCGCCGGT GGTTGAAGAG CCCAAGGGTG GACTAAAAGT AGAAACCGAA
    CCATCGCCGG AAATCCCGTC ACACAGAGCT CGCGCTCCCA ATAAAGGCCT GCGCAAACCC
    AACATTAAAA AAGAACCCAA AACCTCCTCT CCACGACCTC CGCCTGATAA GCGCAAGACG
    AAAGCGGCAA AAGTGCCCAG CAAGTCTCGC GAGTTCATCG AATCGGATTC GTCGACGTCG
    GATTCTGAAG GCAACGAAAG CGACCCATTG CCTTCTCAGA CGCCGAAAGA TTACAGCGAA
    AACCCGTTGG TGCCACTCTC GCCCATTTAT GACCCGCCCA GACTGCTGGT GCAGATCAAT
    CTCAGCCTGC TGACTAGAGT CCCGGGACAG CTCTACAATG AGACGGAGGT TAAGGTGGAG
    CGAGGGTCTC CAGCGGAGGG AAAAAGCAAC CAGAAGCAGC CCGGAGAAAA GATAAGCAAG
    GAAGGAAGGA AGAGGAAACA CAAGAATGAA GATGATGGTG TGAAACCAGA GAGTAAGAAG
    TCCAAACTGG ATGAGAAGTC TTCACGCAAA TCAAGCAGTA ATGATTCTTC CAAGCGTGCT
    CAGGAAAAAG AGAAGGAGTC TGCGCCGTCT CCCTCCATCA CACTGCAGCG GACGCCCAAA
    TCTGAGCAGC AGGGGCGCAA GCGGACCCTC AGCCAGTCCT CCAGCACCGT CCCCAGCAAA
    GACAAGAGCA GCTCCAGCTC CAGACACCGC AGAGGAGACG ACAAACCCCA GCGCAGAGAG
    CCCAAGGAGA AAAGCTCCAA ATCAGAGAAT CCCGTTTCAG TGCCGCCGCT CTCTGAGGGC
    TCCAAATCTA GATCTAAACT TCTGTTTGAG GACAGGGTTC ATTCGGCTGA TCATTACCTT
    CAAGAAGCCA AGAAGTTGAA ACACAATGCG GATGCTCTGG TGAGGATGGA CAGATTTGAG
    AAGGCGGTGT ATTACCTGGA CGCCGTGGTG TCTTTCATCG AGTGTGGAAA TGCCCTGGAG
    AAAAACGCCC AGGAGGCCAA ATCCCCCTTT CCCATGTATG CAGAAACCGT GGAGCTCATC
    AAGTACACTA TGAAGCTAAA GAGCTACATG GCACCAGATG CAACTTCTCA AGACAAAAGG
    CTAGCAGTGC TATGTCTTCG ATGTCAAGCG CTCCTGTATT TGAGGCTTTT CAAGCTGCGG
    AAAGAAAGTG CACTGAAATA CTCTAAAACA CTCACTGAAC ACTTGAAGAA TTCATTGAGT
    AATACCCAAG CTCCGTCTCC TGGAGTAGCA AATAAAAGCG CAGGGATGCC GTCTCCGGTC
    TCCCCCAAAC TCTCTCCTGG CAGTGCGGGC AGCTACTCCT CCAGCAGCTC CAGCCAAAGC
    GCCAGCTCCT CCGTCACCAT CCCGCAGCGC ATCCACCAGA TGGCGGCAAG CTACGTGCAA
    GTCACCTCCA ACTTCCTGTA CGCCACCGAG GTGTGGGACC AGGCTGAGCA GCTCGCCAAA
    GAGCAGAGAG AGTTCTTCAC TGAGCTGGAC AAGGCGATGG GTCCGCTGAT CTTCAACACC
    AGCAGCATGA CTGACCTGGT GCGGTTCACG CGGCAGGGCC TGCACTGGCT ACGACTGGAC
    GCCAAGCTCA TCCAGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa26233
    Clone ID Related Accession (Same CDS sequence) XM_005173900.4 , XM_005173900.3
    Accession Version XM_005173900.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3252bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product AF4/FMR2 family member 4 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007132.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005173900.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    ATGGCCTCTC AGTCAGGCAA CATGAACCGT GAAGACCGGA ATGTGCTCCG TATGAAAGAA 
    AGGGAAAGGA GAAATCAAGA AATCCAGCAG GGAGGAGAGG CCTTTCCAGC TAACTCCCCC
    CTCTTTCCTG AACCTATTAA AGTTGCCAAG GAAGATAAAC TCTCAAGTCG CATCCAGAGC
    ATGCTGGGAA ATTACGACGA GATGAAGGAA ACCATTGGTG AACCGCCAAT GTCCAAGCTC
    ATTTCCAAAG CTTCCAGCTC TTCCTCCGAG GATAAATCTG GCCAGTATGG TGACCAGCGC
    GGAGGCTCTC AGACTCAAAG CAGCAAGTGG ACCCCGGTGG GTCCGGCCTC AGGAGGTGGC
    AACTCTTCAT CGTCTTCTCA GAAACGCTCA ACTGTGCAGA GCGTCAGCCA GAAGAGCAGC
    TCCAGCGGGC AGCGGCACGA GCGGGACTAC AGCGGCAGCA AGAAATCCAG CAAGCACAGC
    AACGAGCACA AGTCCCACTC CAGTTCCAGC CCGGCCAAAG TCTCCAGCAA CCACTCGCGC
    CGAGTCCCCA AGTCCCCCAG AGACAGAGAG GCCAATTGGG ACTCGCCCTC CATCCCGCAT
    TCCTTCCCCA GTGGACATCC CAACCAAACC TTCCCACCCT CGCTCATCAA ATCCAGCTCC
    ATGCCCCAGA AGCCCACCGC TTACGTTCGG CCCATGGACG GCCAGGAGAC GACCGAGGCA
    AAGACGTCTT CAGATTCGTA CAGCGGCCAA TCGCACAGCA GCACAGTTGG AGAAATGAAG
    CCCAATGGAA AGGGCCCACT CCCCAAGCTC AAGATCCCAA CACAGCCTCC AGAGGCGGGG
    GATGCGAGCT GTGTTGATGA GATTTTAAAG GAAATGACTC AGGTCTGGCC TCCTCCATTA
    ACAGCCATTC ACACCCCCTG CAAGACCGAG CCGCCTTCCA AATTCCCCTT CTCCAACGCT
    AAGGATGCCC AGCACCAGTC TGTTGGGGCT CAAAAAAAGT TCTCAGGGAA GAGCGCATCA
    ACCAGTCACC CGTCAAAGCC ATGTGACAGC GAGCAGTCCA ACATGCTTCA TGATGATCTT
    AAGCTCAGCA GCAGTGAAGA CAGTGACGGA GAGCAAGATC CTGCCAAAAA CACCCCTCAA
    AACAACTCCA CAAGTAACAA CAGTAATAAC AGTGAGGGAG CGGAGCGAGA GGACACCAGC
    AGTCACAGCG GCTCCGAGAG CAGCTCCGGG TCCGACAGCG AGAGCGAGAG CAGCTCCACC
    GACAGCGAGA CCACCGAACC GCCACGAGCT GCGACCCCCG AGCGGGAACA GCCTACGGCT
    AACAAATGGC TTCTGGACAA CTGGTTAAAT AAGGTCAACA AGCTGTCTCC TGCTTCTCCG
    GTGGAAAACA GCAGCAGCAG CACGCCGGGA ACCAAGTATA AGAAGGAGGG TCGAGATACC
    AGCTCCAGCA GGAGTTACAG TGGGCAGGGG TCTAAAGAAG CTGCAACGTC AGCCGGCCGA
    GAGTTGCGAC CCAATCAGAA GAGCTCCGAA AGCTGGCGAG GACGCAGTCA GAAATCTCCT
    GCTCAGAGCG AGGGCACAAG TCAATCCGGG CGACGGGTCG TAGGCAAAAA ACAGCCCAAA
    AAACCTGAGA AGCCGCCGGT GGTTGAAGAG CCCAAGGGTG GACTAAAAGT AGAAACCGAA
    CCATCGCCGG AAATCCCGTC ACACAGAGCT CGCGCTCCCA ATAAAGGCCT GCGCAAACCC
    AACATTAAAA AAGAACCCAA AACCTCCTCT CCACGACCTC CGCCTGATAA GCGCAAGACG
    AAAGCGGCAA AAGTGCCCAG CAAGTCTCGC GAGTTCATCG AATCGGATTC GTCGACGTCG
    GATTCTGAAG GCAACGAAAG CGACCCATTG CCTTCTCAGA CGCCGAAAGA TTACAGCGAA
    AACCCGTTGG TGCCACTCTC GCCCATTTAT GACCCGCCCA GACTGCTGGT GCAGATCAAT
    CTCAGCCTGC TGACTAGAGT CCCGGGACAG CTCTACAATG AGACGGAGGT TAAGGTGGAG
    CGAGGGTCTC CAGCGGAGGG AAAAAGCAAC CAGAAGCAGC CCGGAGAAAA GATAAGCAAG
    GAAGGAAGGA AGAGGAAACA CAAGAATGAA GATGATGGTG TGAAACCAGA GAGTAAGAAG
    TCCAAACTGG ATGAGAAGTC TTCACGCAAA TCAAGCAGTA ATGATTCTTC CAAGCGTGCT
    CAGGAAAAAG AGAAGGAGTC TGCGCCGTCT CCCTCCATCA CACTGCAGCG GACGCCCAAA
    TCTGAGCAGC AGGGGCGCAA GCGGACCCTC AGCCAGTCCT CCAGCACCGT CCCCAGCAAA
    GACAAGAGCA GCTCCAGCTC CAGACACCGC AGAGGAGACG ACAAACCCCA GCGCAGAGAG
    CCCAAGGAGA AAAGCTCCAA ATCAGAGAAT CCCGTTTCAG TGCCGCCGCT CTCTGAGGGC
    TCCAAATCTA GATCTAAACT TCTGTTTGAG GACAGGGTTC ATTCGGCTGA TCATTACCTT
    CAAGAAGCCA AGAAGTTGAA ACACAATGCG GATGCTCTGA TGGACAGATT TGAGAAGGCG
    GTGTATTACC TGGACGCCGT GGTGTCTTTC ATCGAGTGTG GAAATGCCCT GGAGAAAAAC
    GCCCAGGAGG CCAAATCCCC CTTTCCCATG TATGCAGAAA CCGTGGAGCT CATCAAGTAC
    ACTATGAAGC TAAAGAGCTA CATGGCACCA GATGCAACTT CTCAAGACAA AAGGCTAGCA
    GTGCTATGTC TTCGATGTCA AGCGCTCCTG TATTTGAGGC TTTTCAAGCT GCGGAAAGAA
    AGTGCACTGA AATACTCTAA AACACTCACT GAACACTTGA AGAATTCATT GAGTAATACC
    CAAGCTCCGT CTCCTGGAGT AGCAAATAAA AGCGCAGGGA TGCCGTCTCC GGTCTCCCCC
    AAACTCTCTC CTGGCAGTGC GGGCAGCTAC TCCTCCAGCA GCTCCAGCCA AAGCGCCAGC
    TCCTCCGTCA CCATCCCGCA GCGCATCCAC CAGATGGCGG CAAGCTACGT GCAAGTCACC
    TCCAACTTCC TGTACGCCAC CGAGGTGTGG GACCAGGCTG AGCAGCTCGC CAAAGAGCAG
    AGAGAGTTCT TCACTGAGCT GGACAAGGCG ATGGGTCCGC TGATCTTCAA CACCAGCAGC
    ATGACTGACC TGGTGCGGTT CACGCGGCAG GGCCTGCACT GGCTACGACT GGACGCCAAG
    CTCATCCAGT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005173957.1
    CDS476..3727
    Translation

    Target ORF information:

    RefSeq Version XM_005173900.4
    Organism Danio rerio(zebrafish)
    Definition Danio rerio AF4/FMR2 family, member 4 (aff4), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005173900.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    ATGGCCTCTC AGTCAGGCAA CATGAACCGT GAAGACCGGA ATGTGCTCCG TATGAAAGAA 
    AGGGAAAGGA GAAATCAAGA AATCCAGCAG GGAGGAGAGG CCTTTCCAGC TAACTCCCCC
    CTCTTTCCTG AACCTATTAA AGTTGCCAAG GAAGATAAAC TCTCAAGTCG CATCCAGAGC
    ATGCTGGGAA ATTACGACGA GATGAAGGAA ACCATTGGTG AACCGCCAAT GTCCAAGCTC
    ATTTCCAAAG CTTCCAGCTC TTCCTCCGAG GATAAATCTG GCCAGTATGG TGACCAGCGC
    GGAGGCTCTC AGACTCAAAG CAGCAAGTGG ACCCCGGTGG GTCCGGCCTC AGGAGGTGGC
    AACTCTTCAT CGTCTTCTCA GAAACGCTCA ACTGTGCAGA GCGTCAGCCA GAAGAGCAGC
    TCCAGCGGGC AGCGGCACGA GCGGGACTAC AGCGGCAGCA AGAAATCCAG CAAGCACAGC
    AACGAGCACA AGTCCCACTC CAGTTCCAGC CCGGCCAAAG TCTCCAGCAA CCACTCGCGC
    CGAGTCCCCA AGTCCCCCAG AGACAGAGAG GCCAATTGGG ACTCGCCCTC CATCCCGCAT
    TCCTTCCCCA GTGGACATCC CAACCAAACC TTCCCACCCT CGCTCATCAA ATCCAGCTCC
    ATGCCCCAGA AGCCCACCGC TTACGTTCGG CCCATGGACG GCCAGGAGAC GACCGAGGCA
    AAGACGTCTT CAGATTCGTA CAGCGGCCAA TCGCACAGCA GCACAGTTGG AGAAATGAAG
    CCCAATGGAA AGGGCCCACT CCCCAAGCTC AAGATCCCAA CACAGCCTCC AGAGGCGGGG
    GATGCGAGCT GTGTTGATGA GATTTTAAAG GAAATGACTC AGGTCTGGCC TCCTCCATTA
    ACAGCCATTC ACACCCCCTG CAAGACCGAG CCGCCTTCCA AATTCCCCTT CTCCAACGCT
    AAGGATGCCC AGCACCAGTC TGTTGGGGCT CAAAAAAAGT TCTCAGGGAA GAGCGCATCA
    ACCAGTCACC CGTCAAAGCC ATGTGACAGC GAGCAGTCCA ACATGCTTCA TGATGATCTT
    AAGCTCAGCA GCAGTGAAGA CAGTGACGGA GAGCAAGATC CTGCCAAAAA CACCCCTCAA
    AACAACTCCA CAAGTAACAA CAGTAATAAC AGTGAGGGAG CGGAGCGAGA GGACACCAGC
    AGTCACAGCG GCTCCGAGAG CAGCTCCGGG TCCGACAGCG AGAGCGAGAG CAGCTCCACC
    GACAGCGAGA CCACCGAACC GCCACGAGCT GCGACCCCCG AGCGGGAACA GCCTACGGCT
    AACAAATGGC TTCTGGACAA CTGGTTAAAT AAGGTCAACA AGCTGTCTCC TGCTTCTCCG
    GTGGAAAACA GCAGCAGCAG CACGCCGGGA ACCAAGTATA AGAAGGAGGG TCGAGATACC
    AGCTCCAGCA GGAGTTACAG TGGGCAGGGG TCTAAAGAAG CTGCAACGTC AGCCGGCCGA
    GAGTTGCGAC CCAATCAGAA GAGCTCCGAA AGCTGGCGAG GACGCAGTCA GAAATCTCCT
    GCTCAGAGCG AGGGCACAAG TCAATCCGGG CGACGGGTCG TAGGCAAAAA ACAGCCCAAA
    AAACCTGAGA AGCCGCCGGT GGTTGAAGAG CCCAAGGGTG GACTAAAAGT AGAAACCGAA
    CCATCGCCGG AAATCCCGTC ACACAGAGCT CGCGCTCCCA ATAAAGGCCT GCGCAAACCC
    AACATTAAAA AAGAACCCAA AACCTCCTCT CCACGACCTC CGCCTGATAA GCGCAAGACG
    AAAGCGGCAA AAGTGCCCAG CAAGTCTCGC GAGTTCATCG AATCGGATTC GTCGACGTCG
    GATTCTGAAG GCAACGAAAG CGACCCATTG CCTTCTCAGA CGCCGAAAGA TTACAGCGAA
    AACCCGTTGG TGCCACTCTC GCCCATTTAT GACCCGCCCA GACTGCTGGT GCAGATCAAT
    CTCAGCCTGC TGACTAGAGT CCCGGGACAG CTCTACAATG AGACGGAGGT TAAGGTGGAG
    CGAGGGTCTC CAGCGGAGGG AAAAAGCAAC CAGAAGCAGC CCGGAGAAAA GATAAGCAAG
    GAAGGAAGGA AGAGGAAACA CAAGAATGAA GATGATGGTG TGAAACCAGA GAGTAAGAAG
    TCCAAACTGG ATGAGAAGTC TTCACGCAAA TCAAGCAGTA ATGATTCTTC CAAGCGTGCT
    CAGGAAAAAG AGAAGGAGTC TGCGCCGTCT CCCTCCATCA CACTGCAGCG GACGCCCAAA
    TCTGAGCAGC AGGGGCGCAA GCGGACCCTC AGCCAGTCCT CCAGCACCGT CCCCAGCAAA
    GACAAGAGCA GCTCCAGCTC CAGACACCGC AGAGGAGACG ACAAACCCCA GCGCAGAGAG
    CCCAAGGAGA AAAGCTCCAA ATCAGAGAAT CCCGTTTCAG TGCCGCCGCT CTCTGAGGGC
    TCCAAATCTA GATCTAAACT TCTGTTTGAG GACAGGGTTC ATTCGGCTGA TCATTACCTT
    CAAGAAGCCA AGAAGTTGAA ACACAATGCG GATGCTCTGA TGGACAGATT TGAGAAGGCG
    GTGTATTACC TGGACGCCGT GGTGTCTTTC ATCGAGTGTG GAAATGCCCT GGAGAAAAAC
    GCCCAGGAGG CCAAATCCCC CTTTCCCATG TATGCAGAAA CCGTGGAGCT CATCAAGTAC
    ACTATGAAGC TAAAGAGCTA CATGGCACCA GATGCAACTT CTCAAGACAA AAGGCTAGCA
    GTGCTATGTC TTCGATGTCA AGCGCTCCTG TATTTGAGGC TTTTCAAGCT GCGGAAAGAA
    AGTGCACTGA AATACTCTAA AACACTCACT GAACACTTGA AGAATTCATT GAGTAATACC
    CAAGCTCCGT CTCCTGGAGT AGCAAATAAA AGCGCAGGGA TGCCGTCTCC GGTCTCCCCC
    AAACTCTCTC CTGGCAGTGC GGGCAGCTAC TCCTCCAGCA GCTCCAGCCA AAGCGCCAGC
    TCCTCCGTCA CCATCCCGCA GCGCATCCAC CAGATGGCGG CAAGCTACGT GCAAGTCACC
    TCCAACTTCC TGTACGCCAC CGAGGTGTGG GACCAGGCTG AGCAGCTCGC CAAAGAGCAG
    AGAGAGTTCT TCACTGAGCT GGACAAGGCG ATGGGTCCGC TGATCTTCAA CACCAGCAGC
    ATGACTGACC TGGTGCGGTT CACGCGGCAG GGCCTGCACT GGCTACGACT GGACGCCAAG
    CTCATCCAGT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa26233
    Clone ID Related Accession (Same CDS sequence) XM_005173900.4 , XM_005173900.3
    Accession Version XM_005173900.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3252bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product AF4/FMR2 family member 4 isoform X2
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007132.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005173900.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    ATGGCCTCTC AGTCAGGCAA CATGAACCGT GAAGACCGGA ATGTGCTCCG TATGAAAGAA 
    AGGGAAAGGA GAAATCAAGA AATCCAGCAG GGAGGAGAGG CCTTTCCAGC TAACTCCCCC
    CTCTTTCCTG AACCTATTAA AGTTGCCAAG GAAGATAAAC TCTCAAGTCG CATCCAGAGC
    ATGCTGGGAA ATTACGACGA GATGAAGGAA ACCATTGGTG AACCGCCAAT GTCCAAGCTC
    ATTTCCAAAG CTTCCAGCTC TTCCTCCGAG GATAAATCTG GCCAGTATGG TGACCAGCGC
    GGAGGCTCTC AGACTCAAAG CAGCAAGTGG ACCCCGGTGG GTCCGGCCTC AGGAGGTGGC
    AACTCTTCAT CGTCTTCTCA GAAACGCTCA ACTGTGCAGA GCGTCAGCCA GAAGAGCAGC
    TCCAGCGGGC AGCGGCACGA GCGGGACTAC AGCGGCAGCA AGAAATCCAG CAAGCACAGC
    AACGAGCACA AGTCCCACTC CAGTTCCAGC CCGGCCAAAG TCTCCAGCAA CCACTCGCGC
    CGAGTCCCCA AGTCCCCCAG AGACAGAGAG GCCAATTGGG ACTCGCCCTC CATCCCGCAT
    TCCTTCCCCA GTGGACATCC CAACCAAACC TTCCCACCCT CGCTCATCAA ATCCAGCTCC
    ATGCCCCAGA AGCCCACCGC TTACGTTCGG CCCATGGACG GCCAGGAGAC GACCGAGGCA
    AAGACGTCTT CAGATTCGTA CAGCGGCCAA TCGCACAGCA GCACAGTTGG AGAAATGAAG
    CCCAATGGAA AGGGCCCACT CCCCAAGCTC AAGATCCCAA CACAGCCTCC AGAGGCGGGG
    GATGCGAGCT GTGTTGATGA GATTTTAAAG GAAATGACTC AGGTCTGGCC TCCTCCATTA
    ACAGCCATTC ACACCCCCTG CAAGACCGAG CCGCCTTCCA AATTCCCCTT CTCCAACGCT
    AAGGATGCCC AGCACCAGTC TGTTGGGGCT CAAAAAAAGT TCTCAGGGAA GAGCGCATCA
    ACCAGTCACC CGTCAAAGCC ATGTGACAGC GAGCAGTCCA ACATGCTTCA TGATGATCTT
    AAGCTCAGCA GCAGTGAAGA CAGTGACGGA GAGCAAGATC CTGCCAAAAA CACCCCTCAA
    AACAACTCCA CAAGTAACAA CAGTAATAAC AGTGAGGGAG CGGAGCGAGA GGACACCAGC
    AGTCACAGCG GCTCCGAGAG CAGCTCCGGG TCCGACAGCG AGAGCGAGAG CAGCTCCACC
    GACAGCGAGA CCACCGAACC GCCACGAGCT GCGACCCCCG AGCGGGAACA GCCTACGGCT
    AACAAATGGC TTCTGGACAA CTGGTTAAAT AAGGTCAACA AGCTGTCTCC TGCTTCTCCG
    GTGGAAAACA GCAGCAGCAG CACGCCGGGA ACCAAGTATA AGAAGGAGGG TCGAGATACC
    AGCTCCAGCA GGAGTTACAG TGGGCAGGGG TCTAAAGAAG CTGCAACGTC AGCCGGCCGA
    GAGTTGCGAC CCAATCAGAA GAGCTCCGAA AGCTGGCGAG GACGCAGTCA GAAATCTCCT
    GCTCAGAGCG AGGGCACAAG TCAATCCGGG CGACGGGTCG TAGGCAAAAA ACAGCCCAAA
    AAACCTGAGA AGCCGCCGGT GGTTGAAGAG CCCAAGGGTG GACTAAAAGT AGAAACCGAA
    CCATCGCCGG AAATCCCGTC ACACAGAGCT CGCGCTCCCA ATAAAGGCCT GCGCAAACCC
    AACATTAAAA AAGAACCCAA AACCTCCTCT CCACGACCTC CGCCTGATAA GCGCAAGACG
    AAAGCGGCAA AAGTGCCCAG CAAGTCTCGC GAGTTCATCG AATCGGATTC GTCGACGTCG
    GATTCTGAAG GCAACGAAAG CGACCCATTG CCTTCTCAGA CGCCGAAAGA TTACAGCGAA
    AACCCGTTGG TGCCACTCTC GCCCATTTAT GACCCGCCCA GACTGCTGGT GCAGATCAAT
    CTCAGCCTGC TGACTAGAGT CCCGGGACAG CTCTACAATG AGACGGAGGT TAAGGTGGAG
    CGAGGGTCTC CAGCGGAGGG AAAAAGCAAC CAGAAGCAGC CCGGAGAAAA GATAAGCAAG
    GAAGGAAGGA AGAGGAAACA CAAGAATGAA GATGATGGTG TGAAACCAGA GAGTAAGAAG
    TCCAAACTGG ATGAGAAGTC TTCACGCAAA TCAAGCAGTA ATGATTCTTC CAAGCGTGCT
    CAGGAAAAAG AGAAGGAGTC TGCGCCGTCT CCCTCCATCA CACTGCAGCG GACGCCCAAA
    TCTGAGCAGC AGGGGCGCAA GCGGACCCTC AGCCAGTCCT CCAGCACCGT CCCCAGCAAA
    GACAAGAGCA GCTCCAGCTC CAGACACCGC AGAGGAGACG ACAAACCCCA GCGCAGAGAG
    CCCAAGGAGA AAAGCTCCAA ATCAGAGAAT CCCGTTTCAG TGCCGCCGCT CTCTGAGGGC
    TCCAAATCTA GATCTAAACT TCTGTTTGAG GACAGGGTTC ATTCGGCTGA TCATTACCTT
    CAAGAAGCCA AGAAGTTGAA ACACAATGCG GATGCTCTGA TGGACAGATT TGAGAAGGCG
    GTGTATTACC TGGACGCCGT GGTGTCTTTC ATCGAGTGTG GAAATGCCCT GGAGAAAAAC
    GCCCAGGAGG CCAAATCCCC CTTTCCCATG TATGCAGAAA CCGTGGAGCT CATCAAGTAC
    ACTATGAAGC TAAAGAGCTA CATGGCACCA GATGCAACTT CTCAAGACAA AAGGCTAGCA
    GTGCTATGTC TTCGATGTCA AGCGCTCCTG TATTTGAGGC TTTTCAAGCT GCGGAAAGAA
    AGTGCACTGA AATACTCTAA AACACTCACT GAACACTTGA AGAATTCATT GAGTAATACC
    CAAGCTCCGT CTCCTGGAGT AGCAAATAAA AGCGCAGGGA TGCCGTCTCC GGTCTCCCCC
    AAACTCTCTC CTGGCAGTGC GGGCAGCTAC TCCTCCAGCA GCTCCAGCCA AAGCGCCAGC
    TCCTCCGTCA CCATCCCGCA GCGCATCCAC CAGATGGCGG CAAGCTACGT GCAAGTCACC
    TCCAACTTCC TGTACGCCAC CGAGGTGTGG GACCAGGCTG AGCAGCTCGC CAAAGAGCAG
    AGAGAGTTCT TCACTGAGCT GGACAAGGCG ATGGGTCCGC TGATCTTCAA CACCAGCAGC
    ATGACTGACC TGGTGCGGTT CACGCGGCAG GGCCTGCACT GGCTACGACT GGACGCCAAG
    CTCATCCAGT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005173957.1
    CDS528..3779
    Translation

    Target ORF information:

    RefSeq Version XM_005173900.3
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio AF4/FMR2 family, member 4 (aff4), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005173900.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    ATGGCCTCTC AGTCAGGCAA CATGAACCGT GAAGACCGGA ATGTGCTCCG TATGAAAGAA 
    AGGGAAAGGA GAAATCAAGA AATCCAGCAG GGAGGAGAGG CCTTTCCAGC TAACTCCCCC
    CTCTTTCCTG AACCTATTAA AGTTGCCAAG GAAGATAAAC TCTCAAGTCG CATCCAGAGC
    ATGCTGGGAA ATTACGACGA GATGAAGGAA ACCATTGGTG AACCGCCAAT GTCCAAGCTC
    ATTTCCAAAG CTTCCAGCTC TTCCTCCGAG GATAAATCTG GCCAGTATGG TGACCAGCGC
    GGAGGCTCTC AGACTCAAAG CAGCAAGTGG ACCCCGGTGG GTCCGGCCTC AGGAGGTGGC
    AACTCTTCAT CGTCTTCTCA GAAACGCTCA ACTGTGCAGA GCGTCAGCCA GAAGAGCAGC
    TCCAGCGGGC AGCGGCACGA GCGGGACTAC AGCGGCAGCA AGAAATCCAG CAAGCACAGC
    AACGAGCACA AGTCCCACTC CAGTTCCAGC CCGGCCAAAG TCTCCAGCAA CCACTCGCGC
    CGAGTCCCCA AGTCCCCCAG AGACAGAGAG GCCAATTGGG ACTCGCCCTC CATCCCGCAT
    TCCTTCCCCA GTGGACATCC CAACCAAACC TTCCCACCCT CGCTCATCAA ATCCAGCTCC
    ATGCCCCAGA AGCCCACCGC TTACGTTCGG CCCATGGACG GCCAGGAGAC GACCGAGGCA
    AAGACGTCTT CAGATTCGTA CAGCGGCCAA TCGCACAGCA GCACAGTTGG AGAAATGAAG
    CCCAATGGAA AGGGCCCACT CCCCAAGCTC AAGATCCCAA CACAGCCTCC AGAGGCGGGG
    GATGCGAGCT GTGTTGATGA GATTTTAAAG GAAATGACTC AGGTCTGGCC TCCTCCATTA
    ACAGCCATTC ACACCCCCTG CAAGACCGAG CCGCCTTCCA AATTCCCCTT CTCCAACGCT
    AAGGATGCCC AGCACCAGTC TGTTGGGGCT CAAAAAAAGT TCTCAGGGAA GAGCGCATCA
    ACCAGTCACC CGTCAAAGCC ATGTGACAGC GAGCAGTCCA ACATGCTTCA TGATGATCTT
    AAGCTCAGCA GCAGTGAAGA CAGTGACGGA GAGCAAGATC CTGCCAAAAA CACCCCTCAA
    AACAACTCCA CAAGTAACAA CAGTAATAAC AGTGAGGGAG CGGAGCGAGA GGACACCAGC
    AGTCACAGCG GCTCCGAGAG CAGCTCCGGG TCCGACAGCG AGAGCGAGAG CAGCTCCACC
    GACAGCGAGA CCACCGAACC GCCACGAGCT GCGACCCCCG AGCGGGAACA GCCTACGGCT
    AACAAATGGC TTCTGGACAA CTGGTTAAAT AAGGTCAACA AGCTGTCTCC TGCTTCTCCG
    GTGGAAAACA GCAGCAGCAG CACGCCGGGA ACCAAGTATA AGAAGGAGGG TCGAGATACC
    AGCTCCAGCA GGAGTTACAG TGGGCAGGGG TCTAAAGAAG CTGCAACGTC AGCCGGCCGA
    GAGTTGCGAC CCAATCAGAA GAGCTCCGAA AGCTGGCGAG GACGCAGTCA GAAATCTCCT
    GCTCAGAGCG AGGGCACAAG TCAATCCGGG CGACGGGTCG TAGGCAAAAA ACAGCCCAAA
    AAACCTGAGA AGCCGCCGGT GGTTGAAGAG CCCAAGGGTG GACTAAAAGT AGAAACCGAA
    CCATCGCCGG AAATCCCGTC ACACAGAGCT CGCGCTCCCA ATAAAGGCCT GCGCAAACCC
    AACATTAAAA AAGAACCCAA AACCTCCTCT CCACGACCTC CGCCTGATAA GCGCAAGACG
    AAAGCGGCAA AAGTGCCCAG CAAGTCTCGC GAGTTCATCG AATCGGATTC GTCGACGTCG
    GATTCTGAAG GCAACGAAAG CGACCCATTG CCTTCTCAGA CGCCGAAAGA TTACAGCGAA
    AACCCGTTGG TGCCACTCTC GCCCATTTAT GACCCGCCCA GACTGCTGGT GCAGATCAAT
    CTCAGCCTGC TGACTAGAGT CCCGGGACAG CTCTACAATG AGACGGAGGT TAAGGTGGAG
    CGAGGGTCTC CAGCGGAGGG AAAAAGCAAC CAGAAGCAGC CCGGAGAAAA GATAAGCAAG
    GAAGGAAGGA AGAGGAAACA CAAGAATGAA GATGATGGTG TGAAACCAGA GAGTAAGAAG
    TCCAAACTGG ATGAGAAGTC TTCACGCAAA TCAAGCAGTA ATGATTCTTC CAAGCGTGCT
    CAGGAAAAAG AGAAGGAGTC TGCGCCGTCT CCCTCCATCA CACTGCAGCG GACGCCCAAA
    TCTGAGCAGC AGGGGCGCAA GCGGACCCTC AGCCAGTCCT CCAGCACCGT CCCCAGCAAA
    GACAAGAGCA GCTCCAGCTC CAGACACCGC AGAGGAGACG ACAAACCCCA GCGCAGAGAG
    CCCAAGGAGA AAAGCTCCAA ATCAGAGAAT CCCGTTTCAG TGCCGCCGCT CTCTGAGGGC
    TCCAAATCTA GATCTAAACT TCTGTTTGAG GACAGGGTTC ATTCGGCTGA TCATTACCTT
    CAAGAAGCCA AGAAGTTGAA ACACAATGCG GATGCTCTGA TGGACAGATT TGAGAAGGCG
    GTGTATTACC TGGACGCCGT GGTGTCTTTC ATCGAGTGTG GAAATGCCCT GGAGAAAAAC
    GCCCAGGAGG CCAAATCCCC CTTTCCCATG TATGCAGAAA CCGTGGAGCT CATCAAGTAC
    ACTATGAAGC TAAAGAGCTA CATGGCACCA GATGCAACTT CTCAAGACAA AAGGCTAGCA
    GTGCTATGTC TTCGATGTCA AGCGCTCCTG TATTTGAGGC TTTTCAAGCT GCGGAAAGAA
    AGTGCACTGA AATACTCTAA AACACTCACT GAACACTTGA AGAATTCATT GAGTAATACC
    CAAGCTCCGT CTCCTGGAGT AGCAAATAAA AGCGCAGGGA TGCCGTCTCC GGTCTCCCCC
    AAACTCTCTC CTGGCAGTGC GGGCAGCTAC TCCTCCAGCA GCTCCAGCCA AAGCGCCAGC
    TCCTCCGTCA CCATCCCGCA GCGCATCCAC CAGATGGCGG CAAGCTACGT GCAAGTCACC
    TCCAACTTCC TGTACGCCAC CGAGGTGTGG GACCAGGCTG AGCAGCTCGC CAAAGAGCAG
    AGAGAGTTCT TCACTGAGCT GGACAAGGCG ATGGGTCCGC TGATCTTCAA CACCAGCAGC
    ATGACTGACC TGGTGCGGTT CACGCGGCAG GGCCTGCACT GGCTACGACT GGACGCCAAG
    CTCATCCAGT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa26234
    Clone ID Related Accession (Same CDS sequence) XM_679235.10 , XM_679235.9
    Accession Version XM_679235.10 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3237bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product AF4/FMR2 family member 4 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007132.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_679235.9. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    ATGAACCGTG AAGACCGGAA TGTGCTCCGT ATGAAAGAAA GGGAAAGGAG AAATCAAGAA 
    ATCCAGCAGG GAGGAGAGGC CTTTCCAGCT AACTCCCCCC TCTTTCCTGA ACCTATTAAA
    GTTGCCAAGG AAGATAAACT CTCAAGTCGC ATCCAGAGCA TGCTGGGAAA TTACGACGAG
    ATGAAGGAAA CCATTGGTGA ACCGCCAATG TCCAAGCTCA TTTCCAAAGC TTCCAGCTCT
    TCCTCCGAGG ATAAATCTGG CCAGTATGGT GACCAGCGCG GAGGCTCTCA GACTCAAAGC
    AGCAAGTGGA CCCCGGTGGG TCCGGCCTCA GGAGGTGGCA ACTCTTCATC GTCTTCTCAG
    AAACGCTCAA CTGTGCAGAG CGTCAGCCAG AAGAGCAGCT CCAGCGGGCA GCGGCACGAG
    CGGGACTACA GCGGCAGCAA GAAATCCAGC AAGCACAGCA ACGAGCACAA GTCCCACTCC
    AGTTCCAGCC CGGCCAAAGT CTCCAGCAAC CACTCGCGCC GAGTCCCCAA GTCCCCCAGA
    GACAGAGAGG CCAATTGGGA CTCGCCCTCC ATCCCGCATT CCTTCCCCAG TGGACATCCC
    AACCAAACCT TCCCACCCTC GCTCATCAAA TCCAGCTCCA TGCCCCAGAA GCCCACCGCT
    TACGTTCGGC CCATGGACGG CCAGGAGACG ACCGAGGCAA AGACGTCTTC AGATTCGTAC
    AGCGGCCAAT CGCACAGCAG CACAGTTGGA GAAATGAAGC CCAATGGAAA GGGCCCACTC
    CCCAAGCTCA AGATCCCAAC ACAGCCTCCA GAGGCGGGGG ATGCGAGCTG TGTTGATGAG
    ATTTTAAAGG AAATGACTCA GGTCTGGCCT CCTCCATTAA CAGCCATTCA CACCCCCTGC
    AAGACCGAGC CGCCTTCCAA ATTCCCCTTC TCCAACGCTA AGGATGCCCA GCACCAGTCT
    GTTGGGGCTC AAAAAAAGTT CTCAGGGAAG AGCGCATCAA CCAGTCACCC GTCAAAGCCA
    TGTGACAGCG AGCAGTCCAA CATGCTTCAT GATGATCTTA AGCTCAGCAG CAGTGAAGAC
    AGTGACGGAG AGCAAGATCC TGCCAAAAAC ACCCCTCAAA ACAACTCCAC AAGTAACAAC
    AGTAATAACA GTGAGGGAGC GGAGCGAGAG GACACCAGCA GTCACAGCGG CTCCGAGAGC
    AGCTCCGGGT CCGACAGCGA GAGCGAGAGC AGCTCCACCG ACAGCGAGAC CACCGAACCG
    CCACGAGCTG CGACCCCCGA GCGGGAACAG CCTACGGCTA ACAAATGGCT TCTGGACAAC
    TGGTTAAATA AGGTCAACAA GCTGTCTCCT GCTTCTCCGG TGGAAAACAG CAGCAGCAGC
    ACGCCGGGAA CCAAGTATAA GAAGGAGGGT CGAGATACCA GCTCCAGCAG GAGTTACAGT
    GGGCAGGGGT CTAAAGAAGC TGCAACGTCA GCCGGCCGAG AGTTGCGACC CAATCAGAAG
    AGCTCCGAAA GCTGGCGAGG ACGCAGTCAG AAATCTCCTG CTCAGAGCGA GGGCACAAGT
    CAATCCGGGC GACGGGTCGT AGGCAAAAAA CAGCCCAAAA AACCTGAGAA GCCGCCGGTG
    GTTGAAGAGC CCAAGGGTGG ACTAAAAGTA GAAACCGAAC CATCGCCGGA AATCCCGTCA
    CACAGAGCTC GCGCTCCCAA TAAAGGCCTG CGCAAACCCA ACATTAAAAA AGAACCCAAA
    ACCTCCTCTC CACGACCTCC GCCTGATAAG CGCAAGACGA AAGCGGCAAA AGTGCCCAGC
    AAGTCTCGCG AGTTCATCGA ATCGGATTCG TCGACGTCGG ATTCTGAAGG CAACGAAAGC
    GACCCATTGC CTTCTCAGAC GCCGAAAGAT TACAGCGAAA ACCCGTTGGT GCCACTCTCG
    CCCATTTATG ACCCGCCCAG ACTGCTGGTG CAGATCAATC TCAGCCTGCT GACTAGAGTC
    CCGGGACAGC TCTACAATGA GACGGAGGTT AAGGTGGAGC GAGGGTCTCC AGCGGAGGGA
    AAAAGCAACC AGAAGCAGCC CGGAGAAAAG ATAAGCAAGG AAGGAAGGAA GAGGAAACAC
    AAGAATGAAG ATGATGGTGT GAAACCAGAG AGTAAGAAGT CCAAACTGGA TGAGAAGTCT
    TCACGCAAAT CAAGCAGTAA TGATTCTTCC AAGCGTGCTC AGGAAAAAGA GAAGGAGTCT
    GCGCCGTCTC CCTCCATCAC ACTGCAGCGG ACGCCCAAAT CTGAGCAGCA GGGGCGCAAG
    CGGACCCTCA GCCAGTCCTC CAGCACCGTC CCCAGCAAAG ACAAGAGCAG CTCCAGCTCC
    AGACACCGCA GAGGAGACGA CAAACCCCAG CGCAGAGAGC CCAAGGAGAA AAGCTCCAAA
    TCAGAGAATC CCGTTTCAGT GCCGCCGCTC TCTGAGGGCT CCAAATCTAG ATCTAAACTT
    CTGTTTGAGG ACAGGGTTCA TTCGGCTGAT CATTACCTTC AAGAAGCCAA GAAGTTGAAA
    CACAATGCGG ATGCTCTGGT GAGGATGGAC AGATTTGAGA AGGCGGTGTA TTACCTGGAC
    GCCGTGGTGT CTTTCATCGA GTGTGGAAAT GCCCTGGAGA AAAACGCCCA GGAGGCCAAA
    TCCCCCTTTC CCATGTATGC AGAAACCGTG GAGCTCATCA AGTACACTAT GAAGCTAAAG
    AGCTACATGG CACCAGATGC AACTTCTCAA GACAAAAGGC TAGCAGTGCT ATGTCTTCGA
    TGTCAAGCGC TCCTGTATTT GAGGCTTTTC AAGCTGCGGA AAGAAAGTGC ACTGAAATAC
    TCTAAAACAC TCACTGAACA CTTGAAGAAT TCATTGAGTA ATACCCAAGC TCCGTCTCCT
    GGAGTAGCAA ATAAAAGCGC AGGGATGCCG TCTCCGGTCT CCCCCAAACT CTCTCCTGGC
    AGTGCGGGCA GCTACTCCTC CAGCAGCTCC AGCCAAAGCG CCAGCTCCTC CGTCACCATC
    CCGCAGCGCA TCCACCAGAT GGCGGCAAGC TACGTGCAAG TCACCTCCAA CTTCCTGTAC
    GCCACCGAGG TGTGGGACCA GGCTGAGCAG CTCGCCAAAG AGCAGAGAGA GTTCTTCACT
    GAGCTGGACA AGGCGATGGG TCCGCTGATC TTCAACACCA GCAGCATGAC TGACCTGGTG
    CGGTTCACGC GGCAGGGCCT GCACTGGCTA CGACTGGACG CCAAGCTCAT CCAGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_684327.6
    CDS300..3536
    Translation

    Target ORF information:

    RefSeq Version XM_679235.10
    Organism Danio rerio(zebrafish)
    Definition Danio rerio AF4/FMR2 family, member 4 (aff4), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_679235.10

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    ATGAACCGTG AAGACCGGAA TGTGCTCCGT ATGAAAGAAA GGGAAAGGAG AAATCAAGAA 
    ATCCAGCAGG GAGGAGAGGC CTTTCCAGCT AACTCCCCCC TCTTTCCTGA ACCTATTAAA
    GTTGCCAAGG AAGATAAACT CTCAAGTCGC ATCCAGAGCA TGCTGGGAAA TTACGACGAG
    ATGAAGGAAA CCATTGGTGA ACCGCCAATG TCCAAGCTCA TTTCCAAAGC TTCCAGCTCT
    TCCTCCGAGG ATAAATCTGG CCAGTATGGT GACCAGCGCG GAGGCTCTCA GACTCAAAGC
    AGCAAGTGGA CCCCGGTGGG TCCGGCCTCA GGAGGTGGCA ACTCTTCATC GTCTTCTCAG
    AAACGCTCAA CTGTGCAGAG CGTCAGCCAG AAGAGCAGCT CCAGCGGGCA GCGGCACGAG
    CGGGACTACA GCGGCAGCAA GAAATCCAGC AAGCACAGCA ACGAGCACAA GTCCCACTCC
    AGTTCCAGCC CGGCCAAAGT CTCCAGCAAC CACTCGCGCC GAGTCCCCAA GTCCCCCAGA
    GACAGAGAGG CCAATTGGGA CTCGCCCTCC ATCCCGCATT CCTTCCCCAG TGGACATCCC
    AACCAAACCT TCCCACCCTC GCTCATCAAA TCCAGCTCCA TGCCCCAGAA GCCCACCGCT
    TACGTTCGGC CCATGGACGG CCAGGAGACG ACCGAGGCAA AGACGTCTTC AGATTCGTAC
    AGCGGCCAAT CGCACAGCAG CACAGTTGGA GAAATGAAGC CCAATGGAAA GGGCCCACTC
    CCCAAGCTCA AGATCCCAAC ACAGCCTCCA GAGGCGGGGG ATGCGAGCTG TGTTGATGAG
    ATTTTAAAGG AAATGACTCA GGTCTGGCCT CCTCCATTAA CAGCCATTCA CACCCCCTGC
    AAGACCGAGC CGCCTTCCAA ATTCCCCTTC TCCAACGCTA AGGATGCCCA GCACCAGTCT
    GTTGGGGCTC AAAAAAAGTT CTCAGGGAAG AGCGCATCAA CCAGTCACCC GTCAAAGCCA
    TGTGACAGCG AGCAGTCCAA CATGCTTCAT GATGATCTTA AGCTCAGCAG CAGTGAAGAC
    AGTGACGGAG AGCAAGATCC TGCCAAAAAC ACCCCTCAAA ACAACTCCAC AAGTAACAAC
    AGTAATAACA GTGAGGGAGC GGAGCGAGAG GACACCAGCA GTCACAGCGG CTCCGAGAGC
    AGCTCCGGGT CCGACAGCGA GAGCGAGAGC AGCTCCACCG ACAGCGAGAC CACCGAACCG
    CCACGAGCTG CGACCCCCGA GCGGGAACAG CCTACGGCTA ACAAATGGCT TCTGGACAAC
    TGGTTAAATA AGGTCAACAA GCTGTCTCCT GCTTCTCCGG TGGAAAACAG CAGCAGCAGC
    ACGCCGGGAA CCAAGTATAA GAAGGAGGGT CGAGATACCA GCTCCAGCAG GAGTTACAGT
    GGGCAGGGGT CTAAAGAAGC TGCAACGTCA GCCGGCCGAG AGTTGCGACC CAATCAGAAG
    AGCTCCGAAA GCTGGCGAGG ACGCAGTCAG AAATCTCCTG CTCAGAGCGA GGGCACAAGT
    CAATCCGGGC GACGGGTCGT AGGCAAAAAA CAGCCCAAAA AACCTGAGAA GCCGCCGGTG
    GTTGAAGAGC CCAAGGGTGG ACTAAAAGTA GAAACCGAAC CATCGCCGGA AATCCCGTCA
    CACAGAGCTC GCGCTCCCAA TAAAGGCCTG CGCAAACCCA ACATTAAAAA AGAACCCAAA
    ACCTCCTCTC CACGACCTCC GCCTGATAAG CGCAAGACGA AAGCGGCAAA AGTGCCCAGC
    AAGTCTCGCG AGTTCATCGA ATCGGATTCG TCGACGTCGG ATTCTGAAGG CAACGAAAGC
    GACCCATTGC CTTCTCAGAC GCCGAAAGAT TACAGCGAAA ACCCGTTGGT GCCACTCTCG
    CCCATTTATG ACCCGCCCAG ACTGCTGGTG CAGATCAATC TCAGCCTGCT GACTAGAGTC
    CCGGGACAGC TCTACAATGA GACGGAGGTT AAGGTGGAGC GAGGGTCTCC AGCGGAGGGA
    AAAAGCAACC AGAAGCAGCC CGGAGAAAAG ATAAGCAAGG AAGGAAGGAA GAGGAAACAC
    AAGAATGAAG ATGATGGTGT GAAACCAGAG AGTAAGAAGT CCAAACTGGA TGAGAAGTCT
    TCACGCAAAT CAAGCAGTAA TGATTCTTCC AAGCGTGCTC AGGAAAAAGA GAAGGAGTCT
    GCGCCGTCTC CCTCCATCAC ACTGCAGCGG ACGCCCAAAT CTGAGCAGCA GGGGCGCAAG
    CGGACCCTCA GCCAGTCCTC CAGCACCGTC CCCAGCAAAG ACAAGAGCAG CTCCAGCTCC
    AGACACCGCA GAGGAGACGA CAAACCCCAG CGCAGAGAGC CCAAGGAGAA AAGCTCCAAA
    TCAGAGAATC CCGTTTCAGT GCCGCCGCTC TCTGAGGGCT CCAAATCTAG ATCTAAACTT
    CTGTTTGAGG ACAGGGTTCA TTCGGCTGAT CATTACCTTC AAGAAGCCAA GAAGTTGAAA
    CACAATGCGG ATGCTCTGGT GAGGATGGAC AGATTTGAGA AGGCGGTGTA TTACCTGGAC
    GCCGTGGTGT CTTTCATCGA GTGTGGAAAT GCCCTGGAGA AAAACGCCCA GGAGGCCAAA
    TCCCCCTTTC CCATGTATGC AGAAACCGTG GAGCTCATCA AGTACACTAT GAAGCTAAAG
    AGCTACATGG CACCAGATGC AACTTCTCAA GACAAAAGGC TAGCAGTGCT ATGTCTTCGA
    TGTCAAGCGC TCCTGTATTT GAGGCTTTTC AAGCTGCGGA AAGAAAGTGC ACTGAAATAC
    TCTAAAACAC TCACTGAACA CTTGAAGAAT TCATTGAGTA ATACCCAAGC TCCGTCTCCT
    GGAGTAGCAA ATAAAAGCGC AGGGATGCCG TCTCCGGTCT CCCCCAAACT CTCTCCTGGC
    AGTGCGGGCA GCTACTCCTC CAGCAGCTCC AGCCAAAGCG CCAGCTCCTC CGTCACCATC
    CCGCAGCGCA TCCACCAGAT GGCGGCAAGC TACGTGCAAG TCACCTCCAA CTTCCTGTAC
    GCCACCGAGG TGTGGGACCA GGCTGAGCAG CTCGCCAAAG AGCAGAGAGA GTTCTTCACT
    GAGCTGGACA AGGCGATGGG TCCGCTGATC TTCAACACCA GCAGCATGAC TGACCTGGTG
    CGGTTCACGC GGCAGGGCCT GCACTGGCTA CGACTGGACG CCAAGCTCAT CCAGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa26234
    Clone ID Related Accession (Same CDS sequence) XM_679235.10 , XM_679235.9
    Accession Version XM_679235.9 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3237bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product AF4/FMR2 family member 4 isoform X3
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007132.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_679235.8. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    ATGAACCGTG AAGACCGGAA TGTGCTCCGT ATGAAAGAAA GGGAAAGGAG AAATCAAGAA 
    ATCCAGCAGG GAGGAGAGGC CTTTCCAGCT AACTCCCCCC TCTTTCCTGA ACCTATTAAA
    GTTGCCAAGG AAGATAAACT CTCAAGTCGC ATCCAGAGCA TGCTGGGAAA TTACGACGAG
    ATGAAGGAAA CCATTGGTGA ACCGCCAATG TCCAAGCTCA TTTCCAAAGC TTCCAGCTCT
    TCCTCCGAGG ATAAATCTGG CCAGTATGGT GACCAGCGCG GAGGCTCTCA GACTCAAAGC
    AGCAAGTGGA CCCCGGTGGG TCCGGCCTCA GGAGGTGGCA ACTCTTCATC GTCTTCTCAG
    AAACGCTCAA CTGTGCAGAG CGTCAGCCAG AAGAGCAGCT CCAGCGGGCA GCGGCACGAG
    CGGGACTACA GCGGCAGCAA GAAATCCAGC AAGCACAGCA ACGAGCACAA GTCCCACTCC
    AGTTCCAGCC CGGCCAAAGT CTCCAGCAAC CACTCGCGCC GAGTCCCCAA GTCCCCCAGA
    GACAGAGAGG CCAATTGGGA CTCGCCCTCC ATCCCGCATT CCTTCCCCAG TGGACATCCC
    AACCAAACCT TCCCACCCTC GCTCATCAAA TCCAGCTCCA TGCCCCAGAA GCCCACCGCT
    TACGTTCGGC CCATGGACGG CCAGGAGACG ACCGAGGCAA AGACGTCTTC AGATTCGTAC
    AGCGGCCAAT CGCACAGCAG CACAGTTGGA GAAATGAAGC CCAATGGAAA GGGCCCACTC
    CCCAAGCTCA AGATCCCAAC ACAGCCTCCA GAGGCGGGGG ATGCGAGCTG TGTTGATGAG
    ATTTTAAAGG AAATGACTCA GGTCTGGCCT CCTCCATTAA CAGCCATTCA CACCCCCTGC
    AAGACCGAGC CGCCTTCCAA ATTCCCCTTC TCCAACGCTA AGGATGCCCA GCACCAGTCT
    GTTGGGGCTC AAAAAAAGTT CTCAGGGAAG AGCGCATCAA CCAGTCACCC GTCAAAGCCA
    TGTGACAGCG AGCAGTCCAA CATGCTTCAT GATGATCTTA AGCTCAGCAG CAGTGAAGAC
    AGTGACGGAG AGCAAGATCC TGCCAAAAAC ACCCCTCAAA ACAACTCCAC AAGTAACAAC
    AGTAATAACA GTGAGGGAGC GGAGCGAGAG GACACCAGCA GTCACAGCGG CTCCGAGAGC
    AGCTCCGGGT CCGACAGCGA GAGCGAGAGC AGCTCCACCG ACAGCGAGAC CACCGAACCG
    CCACGAGCTG CGACCCCCGA GCGGGAACAG CCTACGGCTA ACAAATGGCT TCTGGACAAC
    TGGTTAAATA AGGTCAACAA GCTGTCTCCT GCTTCTCCGG TGGAAAACAG CAGCAGCAGC
    ACGCCGGGAA CCAAGTATAA GAAGGAGGGT CGAGATACCA GCTCCAGCAG GAGTTACAGT
    GGGCAGGGGT CTAAAGAAGC TGCAACGTCA GCCGGCCGAG AGTTGCGACC CAATCAGAAG
    AGCTCCGAAA GCTGGCGAGG ACGCAGTCAG AAATCTCCTG CTCAGAGCGA GGGCACAAGT
    CAATCCGGGC GACGGGTCGT AGGCAAAAAA CAGCCCAAAA AACCTGAGAA GCCGCCGGTG
    GTTGAAGAGC CCAAGGGTGG ACTAAAAGTA GAAACCGAAC CATCGCCGGA AATCCCGTCA
    CACAGAGCTC GCGCTCCCAA TAAAGGCCTG CGCAAACCCA ACATTAAAAA AGAACCCAAA
    ACCTCCTCTC CACGACCTCC GCCTGATAAG CGCAAGACGA AAGCGGCAAA AGTGCCCAGC
    AAGTCTCGCG AGTTCATCGA ATCGGATTCG TCGACGTCGG ATTCTGAAGG CAACGAAAGC
    GACCCATTGC CTTCTCAGAC GCCGAAAGAT TACAGCGAAA ACCCGTTGGT GCCACTCTCG
    CCCATTTATG ACCCGCCCAG ACTGCTGGTG CAGATCAATC TCAGCCTGCT GACTAGAGTC
    CCGGGACAGC TCTACAATGA GACGGAGGTT AAGGTGGAGC GAGGGTCTCC AGCGGAGGGA
    AAAAGCAACC AGAAGCAGCC CGGAGAAAAG ATAAGCAAGG AAGGAAGGAA GAGGAAACAC
    AAGAATGAAG ATGATGGTGT GAAACCAGAG AGTAAGAAGT CCAAACTGGA TGAGAAGTCT
    TCACGCAAAT CAAGCAGTAA TGATTCTTCC AAGCGTGCTC AGGAAAAAGA GAAGGAGTCT
    GCGCCGTCTC CCTCCATCAC ACTGCAGCGG ACGCCCAAAT CTGAGCAGCA GGGGCGCAAG
    CGGACCCTCA GCCAGTCCTC CAGCACCGTC CCCAGCAAAG ACAAGAGCAG CTCCAGCTCC
    AGACACCGCA GAGGAGACGA CAAACCCCAG CGCAGAGAGC CCAAGGAGAA AAGCTCCAAA
    TCAGAGAATC CCGTTTCAGT GCCGCCGCTC TCTGAGGGCT CCAAATCTAG ATCTAAACTT
    CTGTTTGAGG ACAGGGTTCA TTCGGCTGAT CATTACCTTC AAGAAGCCAA GAAGTTGAAA
    CACAATGCGG ATGCTCTGGT GAGGATGGAC AGATTTGAGA AGGCGGTGTA TTACCTGGAC
    GCCGTGGTGT CTTTCATCGA GTGTGGAAAT GCCCTGGAGA AAAACGCCCA GGAGGCCAAA
    TCCCCCTTTC CCATGTATGC AGAAACCGTG GAGCTCATCA AGTACACTAT GAAGCTAAAG
    AGCTACATGG CACCAGATGC AACTTCTCAA GACAAAAGGC TAGCAGTGCT ATGTCTTCGA
    TGTCAAGCGC TCCTGTATTT GAGGCTTTTC AAGCTGCGGA AAGAAAGTGC ACTGAAATAC
    TCTAAAACAC TCACTGAACA CTTGAAGAAT TCATTGAGTA ATACCCAAGC TCCGTCTCCT
    GGAGTAGCAA ATAAAAGCGC AGGGATGCCG TCTCCGGTCT CCCCCAAACT CTCTCCTGGC
    AGTGCGGGCA GCTACTCCTC CAGCAGCTCC AGCCAAAGCG CCAGCTCCTC CGTCACCATC
    CCGCAGCGCA TCCACCAGAT GGCGGCAAGC TACGTGCAAG TCACCTCCAA CTTCCTGTAC
    GCCACCGAGG TGTGGGACCA GGCTGAGCAG CTCGCCAAAG AGCAGAGAGA GTTCTTCACT
    GAGCTGGACA AGGCGATGGG TCCGCTGATC TTCAACACCA GCAGCATGAC TGACCTGGTG
    CGGTTCACGC GGCAGGGCCT GCACTGGCTA CGACTGGACG CCAAGCTCAT CCAGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_684327.6
    CDS352..3588
    Translation

    Target ORF information:

    RefSeq Version XM_679235.9
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio AF4/FMR2 family, member 4 (aff4), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_679235.9

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    ATGAACCGTG AAGACCGGAA TGTGCTCCGT ATGAAAGAAA GGGAAAGGAG AAATCAAGAA 
    ATCCAGCAGG GAGGAGAGGC CTTTCCAGCT AACTCCCCCC TCTTTCCTGA ACCTATTAAA
    GTTGCCAAGG AAGATAAACT CTCAAGTCGC ATCCAGAGCA TGCTGGGAAA TTACGACGAG
    ATGAAGGAAA CCATTGGTGA ACCGCCAATG TCCAAGCTCA TTTCCAAAGC TTCCAGCTCT
    TCCTCCGAGG ATAAATCTGG CCAGTATGGT GACCAGCGCG GAGGCTCTCA GACTCAAAGC
    AGCAAGTGGA CCCCGGTGGG TCCGGCCTCA GGAGGTGGCA ACTCTTCATC GTCTTCTCAG
    AAACGCTCAA CTGTGCAGAG CGTCAGCCAG AAGAGCAGCT CCAGCGGGCA GCGGCACGAG
    CGGGACTACA GCGGCAGCAA GAAATCCAGC AAGCACAGCA ACGAGCACAA GTCCCACTCC
    AGTTCCAGCC CGGCCAAAGT CTCCAGCAAC CACTCGCGCC GAGTCCCCAA GTCCCCCAGA
    GACAGAGAGG CCAATTGGGA CTCGCCCTCC ATCCCGCATT CCTTCCCCAG TGGACATCCC
    AACCAAACCT TCCCACCCTC GCTCATCAAA TCCAGCTCCA TGCCCCAGAA GCCCACCGCT
    TACGTTCGGC CCATGGACGG CCAGGAGACG ACCGAGGCAA AGACGTCTTC AGATTCGTAC
    AGCGGCCAAT CGCACAGCAG CACAGTTGGA GAAATGAAGC CCAATGGAAA GGGCCCACTC
    CCCAAGCTCA AGATCCCAAC ACAGCCTCCA GAGGCGGGGG ATGCGAGCTG TGTTGATGAG
    ATTTTAAAGG AAATGACTCA GGTCTGGCCT CCTCCATTAA CAGCCATTCA CACCCCCTGC
    AAGACCGAGC CGCCTTCCAA ATTCCCCTTC TCCAACGCTA AGGATGCCCA GCACCAGTCT
    GTTGGGGCTC AAAAAAAGTT CTCAGGGAAG AGCGCATCAA CCAGTCACCC GTCAAAGCCA
    TGTGACAGCG AGCAGTCCAA CATGCTTCAT GATGATCTTA AGCTCAGCAG CAGTGAAGAC
    AGTGACGGAG AGCAAGATCC TGCCAAAAAC ACCCCTCAAA ACAACTCCAC AAGTAACAAC
    AGTAATAACA GTGAGGGAGC GGAGCGAGAG GACACCAGCA GTCACAGCGG CTCCGAGAGC
    AGCTCCGGGT CCGACAGCGA GAGCGAGAGC AGCTCCACCG ACAGCGAGAC CACCGAACCG
    CCACGAGCTG CGACCCCCGA GCGGGAACAG CCTACGGCTA ACAAATGGCT TCTGGACAAC
    TGGTTAAATA AGGTCAACAA GCTGTCTCCT GCTTCTCCGG TGGAAAACAG CAGCAGCAGC
    ACGCCGGGAA CCAAGTATAA GAAGGAGGGT CGAGATACCA GCTCCAGCAG GAGTTACAGT
    GGGCAGGGGT CTAAAGAAGC TGCAACGTCA GCCGGCCGAG AGTTGCGACC CAATCAGAAG
    AGCTCCGAAA GCTGGCGAGG ACGCAGTCAG AAATCTCCTG CTCAGAGCGA GGGCACAAGT
    CAATCCGGGC GACGGGTCGT AGGCAAAAAA CAGCCCAAAA AACCTGAGAA GCCGCCGGTG
    GTTGAAGAGC CCAAGGGTGG ACTAAAAGTA GAAACCGAAC CATCGCCGGA AATCCCGTCA
    CACAGAGCTC GCGCTCCCAA TAAAGGCCTG CGCAAACCCA ACATTAAAAA AGAACCCAAA
    ACCTCCTCTC CACGACCTCC GCCTGATAAG CGCAAGACGA AAGCGGCAAA AGTGCCCAGC
    AAGTCTCGCG AGTTCATCGA ATCGGATTCG TCGACGTCGG ATTCTGAAGG CAACGAAAGC
    GACCCATTGC CTTCTCAGAC GCCGAAAGAT TACAGCGAAA ACCCGTTGGT GCCACTCTCG
    CCCATTTATG ACCCGCCCAG ACTGCTGGTG CAGATCAATC TCAGCCTGCT GACTAGAGTC
    CCGGGACAGC TCTACAATGA GACGGAGGTT AAGGTGGAGC GAGGGTCTCC AGCGGAGGGA
    AAAAGCAACC AGAAGCAGCC CGGAGAAAAG ATAAGCAAGG AAGGAAGGAA GAGGAAACAC
    AAGAATGAAG ATGATGGTGT GAAACCAGAG AGTAAGAAGT CCAAACTGGA TGAGAAGTCT
    TCACGCAAAT CAAGCAGTAA TGATTCTTCC AAGCGTGCTC AGGAAAAAGA GAAGGAGTCT
    GCGCCGTCTC CCTCCATCAC ACTGCAGCGG ACGCCCAAAT CTGAGCAGCA GGGGCGCAAG
    CGGACCCTCA GCCAGTCCTC CAGCACCGTC CCCAGCAAAG ACAAGAGCAG CTCCAGCTCC
    AGACACCGCA GAGGAGACGA CAAACCCCAG CGCAGAGAGC CCAAGGAGAA AAGCTCCAAA
    TCAGAGAATC CCGTTTCAGT GCCGCCGCTC TCTGAGGGCT CCAAATCTAG ATCTAAACTT
    CTGTTTGAGG ACAGGGTTCA TTCGGCTGAT CATTACCTTC AAGAAGCCAA GAAGTTGAAA
    CACAATGCGG ATGCTCTGGT GAGGATGGAC AGATTTGAGA AGGCGGTGTA TTACCTGGAC
    GCCGTGGTGT CTTTCATCGA GTGTGGAAAT GCCCTGGAGA AAAACGCCCA GGAGGCCAAA
    TCCCCCTTTC CCATGTATGC AGAAACCGTG GAGCTCATCA AGTACACTAT GAAGCTAAAG
    AGCTACATGG CACCAGATGC AACTTCTCAA GACAAAAGGC TAGCAGTGCT ATGTCTTCGA
    TGTCAAGCGC TCCTGTATTT GAGGCTTTTC AAGCTGCGGA AAGAAAGTGC ACTGAAATAC
    TCTAAAACAC TCACTGAACA CTTGAAGAAT TCATTGAGTA ATACCCAAGC TCCGTCTCCT
    GGAGTAGCAA ATAAAAGCGC AGGGATGCCG TCTCCGGTCT CCCCCAAACT CTCTCCTGGC
    AGTGCGGGCA GCTACTCCTC CAGCAGCTCC AGCCAAAGCG CCAGCTCCTC CGTCACCATC
    CCGCAGCGCA TCCACCAGAT GGCGGCAAGC TACGTGCAAG TCACCTCCAA CTTCCTGTAC
    GCCACCGAGG TGTGGGACCA GGCTGAGCAG CTCGCCAAAG AGCAGAGAGA GTTCTTCACT
    GAGCTGGACA AGGCGATGGG TCCGCTGATC TTCAACACCA GCAGCATGAC TGACCTGGTG
    CGGTTCACGC GGCAGGGCCT GCACTGGCTA CGACTGGACG CCAAGCTCAT CCAGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

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