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hcn4 cDNA ORF clone, Danio rerio(zebrafish)

Summary

Gene Symbol hcn4
Entrez Gene ID 557843
Full Name hyperpolarization activated cyclic nucleotide-gated potassium channel 4
Synonyms si:ch211-203b17.1
General protein information
Preferred Names

hyperpolarization activated cyclic nucleotide-gated potassium channel 4

Names

potassium/sodium hyperpolarization-activated cyclic nucleotide-gated channel 3
Gene Type protein-coding
Organism Danio rerio(zebrafish)

Reference Sequences (RefSeq)

mRNA and Protein(s)

mRNA Protein Name
XM_680986.6 XP_686078.4 potassium/sodium hyperpolarization-activated cyclic nucleotide-gated channel 3
XM_680986.7 XP_686078.4 potassium/sodium hyperpolarization-activated cyclic nucleotide-gated channel 3

Pathways from BioSystems


Homologies


HCN4 XP_002804905.1
Mus musculus (house mouse) Hcn4 NP_001074661.1
Danio rerio (zebrafish) hcn4 XP_686078.4
Xenopus tropicalis (tropical clawed frog) hcn4 XP_004916024.1
Pan troglodytes (chimpanzee) HCN4 XP_003952750.1
Bos taurus (cattle) HCN4 XP_002690539.1
Gallus gallus (chicken) HCN4 XP_425050.3
Homo sapiens (human) HCN4 NP_005468.1
Danio rerio (zebrafish) hcn4l XP_684730.5
Rattus norvegicus (Norway rat) Hcn4 NP_067690.1

Gene Ontology

The following hcn4 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the hcn4 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
ODa23006 XM_680986.7
Latest version!		 Danio rerio hyperpolarization activated cyclic nucleotide-gated potassium channel 4 (hcn4), mRNA. pcDNA3.1+/C-(K)DYK or customized vector TBD $629.30
$899.00
View Old Accession Versions >>
ODa23006 XM_680986.6 Danio rerio hyperpolarization activated cyclic nucleotide-gated potassium channel 4 (hcn4), mRNA. pcDNA3.1+/C-(K)DYK or customized vector TBD $629.30
$899.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)


CloneID ODa23006
Clone ID Related Accession (Same CDS sequence) XM_680986.6 , XM_680986.7
Accession Version XM_680986.6 Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 3423bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2016-06-23
Organism Danio rerio(Zebrafish)
Product potassium/sodium hyperpolarization-activated cyclic nucleotide-gated channel 3
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007129.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_680986.5. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
ATGGACAGGT TGCATTCGTC CATGCGTAAA AGGCTCTACA GTTTGCCACA GCACATCGGG 
CAGAAAGCGT CCATCATGGG TGATGCTGAA GACTCAGACA AGGACACCCG GAGGAAAAGC 
ATCCGGATGA AGCCTTTACC GACCAGCGGG GAGATTAAAA GCGGAGAATC CGCGATCATG 
GAGACGGACA TCGGGAGACC CGTGAAGACC AGCTCCAACG GGGACTGCCG GAGGTTCCGC 
GGCAGCCTTT CGTCCATCAC CAGTCGGCAC GTCCACGACT CGGATGTAGC CGAGGAAAGG 
CGTCTGATCA CGGACGGAGA GGTCACTCCT AGCGAGGAGA GCCCTCCTGG AGCCGCGGGT 
GGCGGCGCGG AGCCCGGTGC GCAGAGCGGC GGGGGAGGCG GAGAGGAAGC CGCCGGTTCG 
AGCCAGCATC AGGGCATCCC GGATCAGCAG GCTGGCTTTA TTAAGCTGGA AGGCATCGAG 
CAGATACTGC CGGACGACGA GAGGTATTAT CAAGCGGGCT TCATGCACAG GCAGTTCGGG 
GCGATGCTGC AGCCGGGGGT CAACAAGTTT TCCCTGAGGA TGTTTGGGAG CGAGAAGGCG 
GTGGAGCGGG AGCAGGAACG GGTCAAATCC GCCGGCTTCT GGATTATTCA CCCCTACAGC 
GATTTCAGGT TTTACTGGGA TCTGACTATG CTGCTCCTGA TGGTGGGCAA TCTAATCATC 
ATCCCGGTCG GCATCACCTT CTTTAAAGAT GAGCACACGC CGCCGTGGAT CGTCTTTAAC 
GTGGTCTCCG ACACTTTCTT CCTGATGGAT CTGGTGCTGA ACTTCCGCAC GGGGATCGTA 
AAGGAGGACA ACGCAGAAAT CATCCTGGAC CCTCAGCAGA TCAAGATCAA GTATCTGCGC 
TCGTGGTTCG TGGTGGACTT CATCTCCTCC ATTCCCGTGG ACTATATCTT CCTCATAGTG 
GAGACGCGCA TCGATTCGGA TTTCTACAAG ACGGCGCGAG CTCTGCGGAT CGTCAGATTC 
ACTAAAATCC TCAGTCTGCT GCGGCTGCTG CGGCTCTCGC GCCTCATACG ATACATCCAC 
CAGTGGGAGG AGATTTTTCA TATGACCTAT GACCTGGCCA GTGCAATGGT GCGAATCGTC 
AACTTGATAG GGATGATGCT GCTGCTCTGC CACTGGGACG GATGTCTGCA GTTTCTGGTG 
CCCATGCTGC AAGACTTCCC TGCTGACTGC TGGGTGGCCA AAAACAAGAT GGTGAACGAC 
ACGTGGGGTC AGCAGTATTC CTACGCTCTC TTCAAGGCCA TGAGCCACAT GCTGTGTATA 
GGATATGGCA TGTATCCTCC CGTGGGCATG ACAGACGTGT GGTTGACCAT CCTCAGCATG 
ATCGTGGGTG CCACGTGTTA CGCCATGTTT GTTGGGCACG CCACAGCGCT GATCCAGTCC 
CTGGACTCCT CGCGCAGGCA GTATCAGGAG AAGTATAAGC AGGTGGAGCA GTACATGTCC 
TTCCATAAGC TGCCAGCAGA CATGAGACAG CGGATCCACG ATTACTACGA GCACAGATAC 
CAGGGCAAGA TGTTTGATGA AGAGAGCATT CTGGGAGAAC TCAATGAGCC ACTGAGAGAG 
GAGATCATAA ACTTCAACTG TCGGAAGCTG GTAGCGTCGA TGCCACTGTT CGCCAATGCA 
GATCCTAATT TTGTGACATC GATGCTCACT AAACTTCGCT TCGAGGTCTT CCAGCCGGGC 
GACTACATCA TACGAGAAGG AACCATCGGG AAGAAGATGT ACTTCATCCA GCACGGTGTG 
GTGAGCGTCC TTACCAAAGG AAACAAGGAG ACCAAGCTGT CGGACGGATC CTACTTTGGA 
GAGATCTGTC TGCTGACGCG GGGTAGACGC ACAGCCAGTG TCAGGGCCGA CACATACTGC 
CGTCTGTACT CTCTGTCAGT GGACAACTTC AACGAGGTGC TGGAGGAGTA TCCCATGATG 
AGGAGAGCGT TTGAGACTGT TGCCCTCGAC CGGCTCGACC GCATCGGAAA GAAGAACTCA 
ATTCTCCAGC ACAAAGTCCA GCACGACTTG AATTCAGGAG TGCTGAACTA CCAGGAAAGT 
GAGATCATCC AGCAGATCGT GCAGCATGAT CGGGACATGG CCCACTGTGC AAACCTGCTG 
CAGAGTCCGT CTCCGCCTGC CCCTCCGTCC CCGACTCCGG TCATCTGGGC TCCACTGATC 
CAGGCTCCTC TACAAGCCGC TGCAGCCACC ACATCGGTCG CTATTGCACT AACACACCAC 
CCACATCTAC CCGCCACGCT CTTCCGACCT CCCGTTTCCC TGCTCGGATC CAGAAACGAG 
CCTCCCAGCA GACTCAAGAG ATTTCAGACG GTTGCACCAA GAACAGGATC AACCACTGAC 
TCTCCATCCA CCTCCCCATC AAAGCTTCAT TCAGGAGTGG ATACTCCTCT TCTAGCATCC 
CTTCATACTC AACACTCATC AGTCACCATC ACTCCCAGTG CTACCACCAA TCAACCAGTG 
TCATTCAGAA GCTTCAGTTC TCCATCAGCA TCTCCGACGT TAAGCACGGC GCAGCTTCAT 
CCACAACCGA GACAAAAACA GCCGAGCACG CCCCCTCTAT CAGCACGCCT GCAAGCAGCA 
GGAGCACATC CGCCCGGCAT CCTCACTACA GCCTCATCAA ACACTAGTGC ACTTTCAGCA 
GGACTATCCA CTCATTCCCC TACTCACAGT TATCCTCCAC CAAAACACCC TCAAACGGGC 
TCCCTACAGT TCGCATCCGG AGGAGGGAAG AGTGGGTTAA CCCTGTTTCA CAGTCCGCCA 
CCCGGATCTC CAATCTCCAT CCATTCCCAA GCCCCCGAGA GCTCCACGTC ATCCCAGACG 
CAGCCCTCTT TTTCCAGTTA CCTTGCTTTG GAAAGGTCAG CGCTGGCCTC GATCGCTCAG 
TACGGCTCAG CGAACGCCTC GCCGAGCTAC ACGCCTTTAG CCCTCAGCCC GACTGTTCAG 
AGTCCTGTCA CAGGGAGAAC TTTCCAGTAC AGTGACCCAT CCAGCGCCGC TGGCTCCCAC 
ACGTCCCTGC TCCTCCCGCA GTCCTCCTGT CCAGGCCAGC TCCCGGGTCA CCACTCCTCC 
GGGACGGACT CTCCTCTGGG CCGCTTTTAT GAGGATTTAA ACATACTGTC CAGCTCGCAC 
CCGTCTCTCG GCGTTGAGGG AGCGGGTCAG TCTTCCCCTG GTTATTTATC ACCGTATTTA 
TCACCGACAC TAACGCCCAA ACCCTGTAGC TCGGTTTCAG GGCACATGTC GAGCCCCGTA 
CAGACTTCTC CAGGCCCTCG AGGTCAGGCT CTTTCAGTAG GGACGTCCCC CGCACTTTCT 
CTCCCGCGCA CCTCAGATGG AGATCTAGAA CCGCTGAGAT CCAAACTCCC CTCCAATTTG 
TGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_686078.4
CDS941..4363
Translation

Target ORF information:

RefSeq Version XM_680986.6
Organism Danio rerio(Zebrafish)
Definition Danio rerio hyperpolarization activated cyclic nucleotide-gated potassium channel 4 (hcn4), mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 XM_680986.6

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
ATGGACAGGT TGCATTCGTC CATGCGTAAA AGGCTCTACA GTTTGCCACA GCACATCGGG 
CAGAAAGCGT CCATCATGGG TGATGCTGAA GACTCAGACA AGGACACCCG GAGGAAAAGC 
ATCCGGATGA AGCCTTTACC GACCAGCGGG GAGATTAAAA GCGGAGAATC CGCGATCATG 
GAGACGGACA TCGGGAGACC CGTGAAGACC AGCTCCAACG GGGACTGCCG GAGGTTCCGC 
GGCAGCCTTT CGTCCATCAC CAGTCGGCAC GTCCACGACT CGGATGTAGC CGAGGAAAGG 
CGTCTGATCA CGGACGGAGA GGTCACTCCT AGCGAGGAGA GCCCTCCTGG AGCCGCGGGT 
GGCGGCGCGG AGCCCGGTGC GCAGAGCGGC GGGGGAGGCG GAGAGGAAGC CGCCGGTTCG 
AGCCAGCATC AGGGCATCCC GGATCAGCAG GCTGGCTTTA TTAAGCTGGA AGGCATCGAG 
CAGATACTGC CGGACGACGA GAGGTATTAT CAAGCGGGCT TCATGCACAG GCAGTTCGGG 
GCGATGCTGC AGCCGGGGGT CAACAAGTTT TCCCTGAGGA TGTTTGGGAG CGAGAAGGCG 
GTGGAGCGGG AGCAGGAACG GGTCAAATCC GCCGGCTTCT GGATTATTCA CCCCTACAGC 
GATTTCAGGT TTTACTGGGA TCTGACTATG CTGCTCCTGA TGGTGGGCAA TCTAATCATC 
ATCCCGGTCG GCATCACCTT CTTTAAAGAT GAGCACACGC CGCCGTGGAT CGTCTTTAAC 
GTGGTCTCCG ACACTTTCTT CCTGATGGAT CTGGTGCTGA ACTTCCGCAC GGGGATCGTA 
AAGGAGGACA ACGCAGAAAT CATCCTGGAC CCTCAGCAGA TCAAGATCAA GTATCTGCGC 
TCGTGGTTCG TGGTGGACTT CATCTCCTCC ATTCCCGTGG ACTATATCTT CCTCATAGTG 
GAGACGCGCA TCGATTCGGA TTTCTACAAG ACGGCGCGAG CTCTGCGGAT CGTCAGATTC 
ACTAAAATCC TCAGTCTGCT GCGGCTGCTG CGGCTCTCGC GCCTCATACG ATACATCCAC 
CAGTGGGAGG AGATTTTTCA TATGACCTAT GACCTGGCCA GTGCAATGGT GCGAATCGTC 
AACTTGATAG GGATGATGCT GCTGCTCTGC CACTGGGACG GATGTCTGCA GTTTCTGGTG 
CCCATGCTGC AAGACTTCCC TGCTGACTGC TGGGTGGCCA AAAACAAGAT GGTGAACGAC 
ACGTGGGGTC AGCAGTATTC CTACGCTCTC TTCAAGGCCA TGAGCCACAT GCTGTGTATA 
GGATATGGCA TGTATCCTCC CGTGGGCATG ACAGACGTGT GGTTGACCAT CCTCAGCATG 
ATCGTGGGTG CCACGTGTTA CGCCATGTTT GTTGGGCACG CCACAGCGCT GATCCAGTCC 
CTGGACTCCT CGCGCAGGCA GTATCAGGAG AAGTATAAGC AGGTGGAGCA GTACATGTCC 
TTCCATAAGC TGCCAGCAGA CATGAGACAG CGGATCCACG ATTACTACGA GCACAGATAC 
CAGGGCAAGA TGTTTGATGA AGAGAGCATT CTGGGAGAAC TCAATGAGCC ACTGAGAGAG 
GAGATCATAA ACTTCAACTG TCGGAAGCTG GTAGCGTCGA TGCCACTGTT CGCCAATGCA 
GATCCTAATT TTGTGACATC GATGCTCACT AAACTTCGCT TCGAGGTCTT CCAGCCGGGC 
GACTACATCA TACGAGAAGG AACCATCGGG AAGAAGATGT ACTTCATCCA GCACGGTGTG 
GTGAGCGTCC TTACCAAAGG AAACAAGGAG ACCAAGCTGT CGGACGGATC CTACTTTGGA 
GAGATCTGTC TGCTGACGCG GGGTAGACGC ACAGCCAGTG TCAGGGCCGA CACATACTGC 
CGTCTGTACT CTCTGTCAGT GGACAACTTC AACGAGGTGC TGGAGGAGTA TCCCATGATG 
AGGAGAGCGT TTGAGACTGT TGCCCTCGAC CGGCTCGACC GCATCGGAAA GAAGAACTCA 
ATTCTCCAGC ACAAAGTCCA GCACGACTTG AATTCAGGAG TGCTGAACTA CCAGGAAAGT 
GAGATCATCC AGCAGATCGT GCAGCATGAT CGGGACATGG CCCACTGTGC AAACCTGCTG 
CAGAGTCCGT CTCCGCCTGC CCCTCCGTCC CCGACTCCGG TCATCTGGGC TCCACTGATC 
CAGGCTCCTC TACAAGCCGC TGCAGCCACC ACATCGGTCG CTATTGCACT AACACACCAC 
CCACATCTAC CCGCCACGCT CTTCCGACCT CCCGTTTCCC TGCTCGGATC CAGAAACGAG 
CCTCCCAGCA GACTCAAGAG ATTTCAGACG GTTGCACCAA GAACAGGATC AACCACTGAC 
TCTCCATCCA CCTCCCCATC AAAGCTTCAT TCAGGAGTGG ATACTCCTCT TCTAGCATCC 
CTTCATACTC AACACTCATC AGTCACCATC ACTCCCAGTG CTACCACCAA TCAACCAGTG 
TCATTCAGAA GCTTCAGTTC TCCATCAGCA TCTCCGACGT TAAGCACGGC GCAGCTTCAT 
CCACAACCGA GACAAAAACA GCCGAGCACG CCCCCTCTAT CAGCACGCCT GCAAGCAGCA 
GGAGCACATC CGCCCGGCAT CCTCACTACA GCCTCATCAA ACACTAGTGC ACTTTCAGCA 
GGACTATCCA CTCATTCCCC TACTCACAGT TATCCTCCAC CAAAACACCC TCAAACGGGC 
TCCCTACAGT TCGCATCCGG AGGAGGGAAG AGTGGGTTAA CCCTGTTTCA CAGTCCGCCA 
CCCGGATCTC CAATCTCCAT CCATTCCCAA GCCCCCGAGA GCTCCACGTC ATCCCAGACG 
CAGCCCTCTT TTTCCAGTTA CCTTGCTTTG GAAAGGTCAG CGCTGGCCTC GATCGCTCAG 
TACGGCTCAG CGAACGCCTC GCCGAGCTAC ACGCCTTTAG CCCTCAGCCC GACTGTTCAG 
AGTCCTGTCA CAGGGAGAAC TTTCCAGTAC AGTGACCCAT CCAGCGCCGC TGGCTCCCAC 
ACGTCCCTGC TCCTCCCGCA GTCCTCCTGT CCAGGCCAGC TCCCGGGTCA CCACTCCTCC 
GGGACGGACT CTCCTCTGGG CCGCTTTTAT GAGGATTTAA ACATACTGTC CAGCTCGCAC 
CCGTCTCTCG GCGTTGAGGG AGCGGGTCAG TCTTCCCCTG GTTATTTATC ACCGTATTTA 
TCACCGACAC TAACGCCCAA ACCCTGTAGC TCGGTTTCAG GGCACATGTC GAGCCCCGTA 
CAGACTTCTC CAGGCCCTCG AGGTCAGGCT CTTTCAGTAG GGACGTCCCC CGCACTTTCT 
CTCCCGCGCA CCTCAGATGG AGATCTAGAA CCGCTGAGAT CCAAACTCCC CTCCAATTTG 
TGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID ODa23006
Clone ID Related Accession (Same CDS sequence) XM_680986.6 , XM_680986.7
Accession Version XM_680986.7 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 3423bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2017-06-14
Organism Danio rerio(zebrafish)
Product potassium/sodium hyperpolarization-activated cyclic nucleotide-gated channel 3
Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007129.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_680986.6. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
ATGGACAGGT TGCATTCGTC CATGCGTAAA AGGCTCTACA GTTTGCCACA GCACATCGGG 
CAGAAAGCGT CCATCATGGG TGATGCTGAA GACTCAGACA AGGACACCCG GAGGAAAAGC 
ATCCGGATGA AGCCTTTACC GACCAGCGGG GAGATTAAAA GCGGAGAATC CGCGATCATG 
GAGACGGACA TCGGGAGACC CGTGAAGACC AGCTCCAACG GGGACTGCCG GAGGTTCCGC 
GGCAGCCTTT CGTCCATCAC CAGTCGGCAC GTCCACGACT CGGATGTAGC CGAGGAAAGG 
CGTCTGATCA CGGACGGAGA GGTCACTCCT AGCGAGGAGA GCCCTCCTGG AGCCGCGGGT 
GGCGGCGCGG AGCCCGGTGC GCAGAGCGGC GGGGGAGGCG GAGAGGAAGC CGCCGGTTCG 
AGCCAGCATC AGGGCATCCC GGATCAGCAG GCTGGCTTTA TTAAGCTGGA AGGCATCGAG 
CAGATACTGC CGGACGACGA GAGGTATTAT CAAGCGGGCT TCATGCACAG GCAGTTCGGG 
GCGATGCTGC AGCCGGGGGT CAACAAGTTT TCCCTGAGGA TGTTTGGGAG CGAGAAGGCG 
GTGGAGCGGG AGCAGGAACG GGTCAAATCC GCCGGCTTCT GGATTATTCA CCCCTACAGC 
GATTTCAGGT TTTACTGGGA TCTGACTATG CTGCTCCTGA TGGTGGGCAA TCTAATCATC 
ATCCCGGTCG GCATCACCTT CTTTAAAGAT GAGCACACGC CGCCGTGGAT CGTCTTTAAC 
GTGGTCTCCG ACACTTTCTT CCTGATGGAT CTGGTGCTGA ACTTCCGCAC GGGGATCGTA 
AAGGAGGACA ACGCAGAAAT CATCCTGGAC CCTCAGCAGA TCAAGATCAA GTATCTGCGC 
TCGTGGTTCG TGGTGGACTT CATCTCCTCC ATTCCCGTGG ACTATATCTT CCTCATAGTG 
GAGACGCGCA TCGATTCGGA TTTCTACAAG ACGGCGCGAG CTCTGCGGAT CGTCAGATTC 
ACTAAAATCC TCAGTCTGCT GCGGCTGCTG CGGCTCTCGC GCCTCATACG ATACATCCAC 
CAGTGGGAGG AGATTTTTCA TATGACCTAT GACCTGGCCA GTGCAATGGT GCGAATCGTC 
AACTTGATAG GGATGATGCT GCTGCTCTGC CACTGGGACG GATGTCTGCA GTTTCTGGTG 
CCCATGCTGC AAGACTTCCC TGCTGACTGC TGGGTGGCCA AAAACAAGAT GGTGAACGAC 
ACGTGGGGTC AGCAGTATTC CTACGCTCTC TTCAAGGCCA TGAGCCACAT GCTGTGTATA 
GGATATGGCA TGTATCCTCC CGTGGGCATG ACAGACGTGT GGTTGACCAT CCTCAGCATG 
ATCGTGGGTG CCACGTGTTA CGCCATGTTT GTTGGGCACG CCACAGCGCT GATCCAGTCC 
CTGGACTCCT CGCGCAGGCA GTATCAGGAG AAGTATAAGC AGGTGGAGCA GTACATGTCC 
TTCCATAAGC TGCCAGCAGA CATGAGACAG CGGATCCACG ATTACTACGA GCACAGATAC 
CAGGGCAAGA TGTTTGATGA AGAGAGCATT CTGGGAGAAC TCAATGAGCC ACTGAGAGAG 
GAGATCATAA ACTTCAACTG TCGGAAGCTG GTAGCGTCGA TGCCACTGTT CGCCAATGCA 
GATCCTAATT TTGTGACATC GATGCTCACT AAACTTCGCT TCGAGGTCTT CCAGCCGGGC 
GACTACATCA TACGAGAAGG AACCATCGGG AAGAAGATGT ACTTCATCCA GCACGGTGTG 
GTGAGCGTCC TTACCAAAGG AAACAAGGAG ACCAAGCTGT CGGACGGATC CTACTTTGGA 
GAGATCTGTC TGCTGACGCG GGGTAGACGC ACAGCCAGTG TCAGGGCCGA CACATACTGC 
CGTCTGTACT CTCTGTCAGT GGACAACTTC AACGAGGTGC TGGAGGAGTA TCCCATGATG 
AGGAGAGCGT TTGAGACTGT TGCCCTCGAC CGGCTCGACC GCATCGGAAA GAAGAACTCA 
ATTCTCCAGC ACAAAGTCCA GCACGACTTG AATTCAGGAG TGCTGAACTA CCAGGAAAGT 
GAGATCATCC AGCAGATCGT GCAGCATGAT CGGGACATGG CCCACTGTGC AAACCTGCTG 
CAGAGTCCGT CTCCGCCTGC CCCTCCGTCC CCGACTCCGG TCATCTGGGC TCCACTGATC 
CAGGCTCCTC TACAAGCCGC TGCAGCCACC ACATCGGTCG CTATTGCACT AACACACCAC 
CCACATCTAC CCGCCACGCT CTTCCGACCT CCCGTTTCCC TGCTCGGATC CAGAAACGAG 
CCTCCCAGCA GACTCAAGAG ATTTCAGACG GTTGCACCAA GAACAGGATC AACCACTGAC 
TCTCCATCCA CCTCCCCATC AAAGCTTCAT TCAGGAGTGG ATACTCCTCT TCTAGCATCC 
CTTCATACTC AACACTCATC AGTCACCATC ACTCCCAGTG CTACCACCAA TCAACCAGTG 
TCATTCAGAA GCTTCAGTTC TCCATCAGCA TCTCCGACGT TAAGCACGGC GCAGCTTCAT 
CCACAACCGA GACAAAAACA GCCGAGCACG CCCCCTCTAT CAGCACGCCT GCAAGCAGCA 
GGAGCACATC CGCCCGGCAT CCTCACTACA GCCTCATCAA ACACTAGTGC ACTTTCAGCA 
GGACTATCCA CTCATTCCCC TACTCACAGT TATCCTCCAC CAAAACACCC TCAAACGGGC 
TCCCTACAGT TCGCATCCGG AGGAGGGAAG AGTGGGTTAA CCCTGTTTCA CAGTCCGCCA 
CCCGGATCTC CAATCTCCAT CCATTCCCAA GCCCCCGAGA GCTCCACGTC ATCCCAGACG 
CAGCCCTCTT TTTCCAGTTA CCTTGCTTTG GAAAGGTCAG CGCTGGCCTC GATCGCTCAG 
TACGGCTCAG CGAACGCCTC GCCGAGCTAC ACGCCTTTAG CCCTCAGCCC GACTGTTCAG 
AGTCCTGTCA CAGGGAGAAC TTTCCAGTAC AGTGACCCAT CCAGCGCCGC TGGCTCCCAC 
ACGTCCCTGC TCCTCCCGCA GTCCTCCTGT CCAGGCCAGC TCCCGGGTCA CCACTCCTCC 
GGGACGGACT CTCCTCTGGG CCGCTTTTAT GAGGATTTAA ACATACTGTC CAGCTCGCAC 
CCGTCTCTCG GCGTTGAGGG AGCGGGTCAG TCTTCCCCTG GTTATTTATC ACCGTATTTA 
TCACCGACAC TAACGCCCAA ACCCTGTAGC TCGGTTTCAG GGCACATGTC GAGCCCCGTA 
CAGACTTCTC CAGGCCCTCG AGGTCAGGCT CTTTCAGTAG GGACGTCCCC CGCACTTTCT 
CTCCCGCGCA CCTCAGATGG AGATCTAGAA CCGCTGAGAT CCAAACTCCC CTCCAATTTG 
TGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_686078.4
CDS933..4355
Translation

Target ORF information:

RefSeq Version XM_680986.7
Organism Danio rerio(zebrafish)
Definition Danio rerio hyperpolarization activated cyclic nucleotide-gated potassium channel 4 (hcn4), mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 XM_680986.7

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
ATGGACAGGT TGCATTCGTC CATGCGTAAA AGGCTCTACA GTTTGCCACA GCACATCGGG 
CAGAAAGCGT CCATCATGGG TGATGCTGAA GACTCAGACA AGGACACCCG GAGGAAAAGC 
ATCCGGATGA AGCCTTTACC GACCAGCGGG GAGATTAAAA GCGGAGAATC CGCGATCATG 
GAGACGGACA TCGGGAGACC CGTGAAGACC AGCTCCAACG GGGACTGCCG GAGGTTCCGC 
GGCAGCCTTT CGTCCATCAC CAGTCGGCAC GTCCACGACT CGGATGTAGC CGAGGAAAGG 
CGTCTGATCA CGGACGGAGA GGTCACTCCT AGCGAGGAGA GCCCTCCTGG AGCCGCGGGT 
GGCGGCGCGG AGCCCGGTGC GCAGAGCGGC GGGGGAGGCG GAGAGGAAGC CGCCGGTTCG 
AGCCAGCATC AGGGCATCCC GGATCAGCAG GCTGGCTTTA TTAAGCTGGA AGGCATCGAG 
CAGATACTGC CGGACGACGA GAGGTATTAT CAAGCGGGCT TCATGCACAG GCAGTTCGGG 
GCGATGCTGC AGCCGGGGGT CAACAAGTTT TCCCTGAGGA TGTTTGGGAG CGAGAAGGCG 
GTGGAGCGGG AGCAGGAACG GGTCAAATCC GCCGGCTTCT GGATTATTCA CCCCTACAGC 
GATTTCAGGT TTTACTGGGA TCTGACTATG CTGCTCCTGA TGGTGGGCAA TCTAATCATC 
ATCCCGGTCG GCATCACCTT CTTTAAAGAT GAGCACACGC CGCCGTGGAT CGTCTTTAAC 
GTGGTCTCCG ACACTTTCTT CCTGATGGAT CTGGTGCTGA ACTTCCGCAC GGGGATCGTA 
AAGGAGGACA ACGCAGAAAT CATCCTGGAC CCTCAGCAGA TCAAGATCAA GTATCTGCGC 
TCGTGGTTCG TGGTGGACTT CATCTCCTCC ATTCCCGTGG ACTATATCTT CCTCATAGTG 
GAGACGCGCA TCGATTCGGA TTTCTACAAG ACGGCGCGAG CTCTGCGGAT CGTCAGATTC 
ACTAAAATCC TCAGTCTGCT GCGGCTGCTG CGGCTCTCGC GCCTCATACG ATACATCCAC 
CAGTGGGAGG AGATTTTTCA TATGACCTAT GACCTGGCCA GTGCAATGGT GCGAATCGTC 
AACTTGATAG GGATGATGCT GCTGCTCTGC CACTGGGACG GATGTCTGCA GTTTCTGGTG 
CCCATGCTGC AAGACTTCCC TGCTGACTGC TGGGTGGCCA AAAACAAGAT GGTGAACGAC 
ACGTGGGGTC AGCAGTATTC CTACGCTCTC TTCAAGGCCA TGAGCCACAT GCTGTGTATA 
GGATATGGCA TGTATCCTCC CGTGGGCATG ACAGACGTGT GGTTGACCAT CCTCAGCATG 
ATCGTGGGTG CCACGTGTTA CGCCATGTTT GTTGGGCACG CCACAGCGCT GATCCAGTCC 
CTGGACTCCT CGCGCAGGCA GTATCAGGAG AAGTATAAGC AGGTGGAGCA GTACATGTCC 
TTCCATAAGC TGCCAGCAGA CATGAGACAG CGGATCCACG ATTACTACGA GCACAGATAC 
CAGGGCAAGA TGTTTGATGA AGAGAGCATT CTGGGAGAAC TCAATGAGCC ACTGAGAGAG 
GAGATCATAA ACTTCAACTG TCGGAAGCTG GTAGCGTCGA TGCCACTGTT CGCCAATGCA 
GATCCTAATT TTGTGACATC GATGCTCACT AAACTTCGCT TCGAGGTCTT CCAGCCGGGC 
GACTACATCA TACGAGAAGG AACCATCGGG AAGAAGATGT ACTTCATCCA GCACGGTGTG 
GTGAGCGTCC TTACCAAAGG AAACAAGGAG ACCAAGCTGT CGGACGGATC CTACTTTGGA 
GAGATCTGTC TGCTGACGCG GGGTAGACGC ACAGCCAGTG TCAGGGCCGA CACATACTGC 
CGTCTGTACT CTCTGTCAGT GGACAACTTC AACGAGGTGC TGGAGGAGTA TCCCATGATG 
AGGAGAGCGT TTGAGACTGT TGCCCTCGAC CGGCTCGACC GCATCGGAAA GAAGAACTCA 
ATTCTCCAGC ACAAAGTCCA GCACGACTTG AATTCAGGAG TGCTGAACTA CCAGGAAAGT 
GAGATCATCC AGCAGATCGT GCAGCATGAT CGGGACATGG CCCACTGTGC AAACCTGCTG 
CAGAGTCCGT CTCCGCCTGC CCCTCCGTCC CCGACTCCGG TCATCTGGGC TCCACTGATC 
CAGGCTCCTC TACAAGCCGC TGCAGCCACC ACATCGGTCG CTATTGCACT AACACACCAC 
CCACATCTAC CCGCCACGCT CTTCCGACCT CCCGTTTCCC TGCTCGGATC CAGAAACGAG 
CCTCCCAGCA GACTCAAGAG ATTTCAGACG GTTGCACCAA GAACAGGATC AACCACTGAC 
TCTCCATCCA CCTCCCCATC AAAGCTTCAT TCAGGAGTGG ATACTCCTCT TCTAGCATCC 
CTTCATACTC AACACTCATC AGTCACCATC ACTCCCAGTG CTACCACCAA TCAACCAGTG 
TCATTCAGAA GCTTCAGTTC TCCATCAGCA TCTCCGACGT TAAGCACGGC GCAGCTTCAT 
CCACAACCGA GACAAAAACA GCCGAGCACG CCCCCTCTAT CAGCACGCCT GCAAGCAGCA 
GGAGCACATC CGCCCGGCAT CCTCACTACA GCCTCATCAA ACACTAGTGC ACTTTCAGCA 
GGACTATCCA CTCATTCCCC TACTCACAGT TATCCTCCAC CAAAACACCC TCAAACGGGC 
TCCCTACAGT TCGCATCCGG AGGAGGGAAG AGTGGGTTAA CCCTGTTTCA CAGTCCGCCA 
CCCGGATCTC CAATCTCCAT CCATTCCCAA GCCCCCGAGA GCTCCACGTC ATCCCAGACG 
CAGCCCTCTT TTTCCAGTTA CCTTGCTTTG GAAAGGTCAG CGCTGGCCTC GATCGCTCAG 
TACGGCTCAG CGAACGCCTC GCCGAGCTAC ACGCCTTTAG CCCTCAGCCC GACTGTTCAG 
AGTCCTGTCA CAGGGAGAAC TTTCCAGTAC AGTGACCCAT CCAGCGCCGC TGGCTCCCAC 
ACGTCCCTGC TCCTCCCGCA GTCCTCCTGT CCAGGCCAGC TCCCGGGTCA CCACTCCTCC 
GGGACGGACT CTCCTCTGGG CCGCTTTTAT GAGGATTTAA ACATACTGTC CAGCTCGCAC 
CCGTCTCTCG GCGTTGAGGG AGCGGGTCAG TCTTCCCCTG GTTATTTATC ACCGTATTTA 
TCACCGACAC TAACGCCCAA ACCCTGTAGC TCGGTTTCAG GGCACATGTC GAGCCCCGTA 
CAGACTTCTC CAGGCCCTCG AGGTCAGGCT CTTTCAGTAG GGACGTCCCC CGCACTTTCT 
CTCCCGCGCA CCTCAGATGG AGATCTAGAA CCGCTGAGAT CCAAACTCCC CTCCAATTTG 
TGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

PubMed