mprip cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	mprip
Entrez Gene ID	558831
Full Name	myosin phosphatase Rho interacting protein
Synonyms	wu:fd08a03,zgc:175222
General protein information	Preferred Names myosin phosphatase Rho interacting protein Names myosin phosphatase Rho-interacting protein myosin phosphatase-Rho interacting protein
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
XM_009306362.2	XP_009304637.1	myosin phosphatase Rho-interacting protein isoform X2
XM_021480198.1	XP_021335873.1	myosin phosphatase Rho-interacting protein isoform X2
XM_009306364.3	XP_009304639.1	myosin phosphatase Rho-interacting protein isoform X3
XM_009306361.2	XP_009304636.1	myosin phosphatase Rho-interacting protein isoform X1
XM_009306364.2	XP_009304639.1	myosin phosphatase Rho-interacting protein isoform X4
XM_021480197.1	XP_021335872.1	myosin phosphatase Rho-interacting protein isoform X2
XM_009306363.2	XP_009304638.1	myosin phosphatase Rho-interacting protein isoform X3
XM_009306361.3	XP_009304636.1	myosin phosphatase Rho-interacting protein isoform X1

Pathways from BioSystems
Gene Ontology

The following mprip gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the mprip cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODa17461	XM_009306364.3 Latest version!	Danio rerio myosin phosphatase Rho interacting protein (mprip), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
ODa42923	XM_009306363.2 Latest version!	Danio rerio myosin phosphatase Rho interacting protein (mprip), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
ODa42921	XM_009306361.3 Latest version!	Danio rerio myosin phosphatase Rho interacting protein (mprip), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
ODa42922	XM_009306362.2 Latest version!	Danio rerio myosin phosphatase Rho interacting protein (mprip), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
ODa42922	XM_021480197.1 Latest version!	Danio rerio myosin phosphatase Rho interacting protein (mprip), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
ODa42922	XM_021480198.1 Latest version!	Danio rerio myosin phosphatase Rho interacting protein (mprip), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
View Old Accession Versions >>
ODa17461	XM_009306364.2	Danio rerio myosin phosphatase Rho interacting protein (mprip), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
ODa42921	XM_009306361.2	Danio rerio myosin phosphatase Rho interacting protein (mprip), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

mprip ( XM_009306364.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009304639.1 Danio rerio myosin phosphatase Rho interacting protein (mprip), transcript variant X4, mRNA.

Price & Availability↑

CloneID	ODa17461
Clone ID Related Accession (Same CDS sequence)	XM_009306364.3 , XM_009306364.2
Accession Version	XM_009306364.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3186bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	myosin phosphatase Rho-interacting protein isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007123.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009306364.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGAGCG CGAAGGAAAA CACGTGTAGG AAATTTCAGG CGAACATTTT CAACAAAAGC 
AAGTGTCAGA ACTGCTTCAA GCCGCGAGAG CTGCATCTGC TGACGGATCA GGATCTCAAT 
CAGGCCAAGC CCATCTATGG TGGATGGCTG TGTTTGGCTC CGGAGGGGAC GGATTTTGAC 
AACCCCATGC AGAGATCACG GAAATGGCAG CGGCGATTCT TCGTCCTGTA TGAGCACGGC 
TGCTTGCGCT TCGCTCTGGA TGAATCGCCC AGCACGCTGC CGCAGGGAAC GGTGAACATG 
AACCAGTGCA CAGACGTGGT GGACGCGGAG CCGCGCACGG GCCAGAAGAA CGCGCTGTGC 
ATCGTGACGC CCGAGCAGGA GTATTTCATC CGCGGAGACA ATAAAGAGAT CATCAACGGA 
TGGAGTGAGC AGCTAGCGGT GTATCCCAGA ACCAACAAGC AGAACCAGAA GAAGAAGCGC 
AAGGTGGAGC CGGCCACCTC ACAGGAGCCG GGTCCGGCTA AGGTGGCGGT GACGGGCGCC 
GGCATCCCTG ACGCAGAGAA AACTCCAGAC TCCAGCTCCA TCATCTGGCA GGAGGAGCTG 
CAGAGCAGAG AAGCAGACGC CGCTCACACC TGGAGCAGCG AGATTCCTCC TGTCGGCTCA 
CCGCTGCCCT CTACAGGCGA GGGGTCGACG GTGAGCTCAG TGAATGGGGA TGAGGTTGAC 
CGCTGCGGAC TTTCCTTCCA CTCGCCGGTC TCTCAGGCTC CTGTAGACCC GCTGTCCCCG 
ACAGGCAGCA GCTCCAGCAG GCAGAGTGCA GAGACGGGCG GGGTCCCTCG CTGCCTATCC 
CCGGCTCCCA GCGACCCCTT CCCCTCAGGC AGCAGCCTCC TGTCCAACGG CTCGCACATC 
AGCGGCTCTA TGAGCTCTCT GGACTCGGAG GCTAGCGGCA GCACCGTCAC GAGCAGCGAC 
AGCCACCCGC CGGAGCCCCG CGCCCTCAAA CGAAGCCGCC TACGGAGCCC ACAGACCCGG 
GAGCGGGAGC TAGTGCTGAG CCCTGAGAGA AGCGGCCGCT CGAATGTGAT TGAGAAGCTG 
GAGGCGCTGG AGCTGGAGCA GCAGGAGCGC ATGGAGGTTC AGGAGTCCAG CAGCACTGAG 
GCCCGACAGG GGCGGAGTCA GAACAGGAGA TTCCAGCGGC AGGAGCAGAA GACGGACGCA 
GCACAGGCTC TAGACTTCCA TCCTCCTCTA AGAAGAGCCA AATCTCTGGA CCGCAGGACC 
ACTGAGTCCG TCATGACCCC TGATCTGCTG AACTTTAAGA AAGGCTGGAT GGTGAAGCTG 
GATGAAGAAG GACAGTGGAA GAAGTATTGG TTTGTGCTGA CGGATCACAG TCTGCGATAC 
TACAAGGACT CCATCGCTGA AGAGGCGTCA GATCTGGATG GAGAGATCGA CCTCAGCACA 
TGCTATAATG TTACAGAGTA TCAGGCGCAG AGGAACTACG GCTTCCAGAT TTACACTCAG 
GAGGCGGTGC ACACACTCTC CGCCATGACT GCAGGAATCC GCCGTAACTG GATCCAGGCA 
GTGATGAAGA ATGTCCGGCC AGCCACTGCT CCAGATGTAG CCAGTCTGAC GGATGAGCAT 
GGGTCGTTTG AGGGTGTCTG TCGACCGGAC GTGACGCAGG ACTCTCCCTC CTCTGAGGCG 
TCTGTGGAGC GGGAAACAGG GGCCAAAAAA AGCCGTGCGC GGGAACGCAG GCGAGAGGGG 
CGGAGCAAAA CTTTCGACTG GGCGGAGTTT CGTCCCATCG CTCAGGCATT GGCTCAGCAG 
AGAGCGCAGG AGGCGGAGCT TACAGAGCGC AGCAAGAGGA GAGAGGAGCG AAGGCGGAGG 
TATGAGAGCG TGACCGGAGC AACCTTTGAT CCTGCACTGA TGGAGCAAGA GGGGGGCGTG 
GCTCAGACTG AAGCTCCGCC CCTGTCGGCT GAATGGCAGC AGCGAGTGGA GGATGAGATC 
GAGCAACGCT GGCAGCAGGT GGAGAAAACA CCAATCAGAG ACGAGCGCAG GGTTCCGTTA 
GGCTCCGCCC ACAGCAGAGA CGCCACAGAC ATGGAGAAGC TGCTGGAGGG ATACAAGAGA 
GGGGTGGAGG AGCTCAAGGC TCAGCTGGAG AACTGCCATC AGCAGCTTCT GGACTCCAAT 
CGGCACAAGC AGGATCTGGA GGGAAAACTG AAGATGGCGG TGGAGCGCGA GCAGCAGACG 
CGAGCCGGGT ACATCTCTCC GGCCACATGT GAGCGAGGTT TCGCCGCTAT GGAGGAGTCG 
CACCAGAAGG TGATCGAGGA GCTGCAGAAG AAACACCAGA GAGAGCTGGA GAAGCTGCAG 
GAGGAGAAGG AGAGACTGCT GGAGGAGGAG ACGGCTGCTA CCATCGCCGC TATCGAGGCG 
ATGAAGAACG CACACCGGTC AGAGCTGGAG CGAGAGCTGG AGAAAGCACG CAAAGCCACC 
AGCAGCACAG AGAACGCAGA CATCGAGGAG ATCCACAAAC AGCACGAGGA GGAGCTGGTG 
TCGTATCAGC GGGAGATCGA GGTGCTGTCA GAGCAGTATT CACAGAAGTG TCTGGAGAAC 
GCTCATCTGG CTCAGGCGCT GGAGGCCGAG CGGCAGGCGC TCAGACAGTG CCAGAGGGAG 
AACCAGGAGC TCAATGCACA CAACCAGGAG CTCAACAATC GTCTGGCTGC AGAGATCACC 
AAGATGCGCT CGATGGCGAC TGAAGACGGA GCTGACACTG GAGGACTGAT TCAAGGGAAG 
GAGCTGTACG AGCTGGAGGT GATGCTGAGG GTGAAGGAGT CAGAAGTGCA GTATCTGAAG 
CAGGAGATCA ACTCTCTGAA AGATGAGCTC CAGACGGCAC AAAGAGATAA GAAATATGCA 
ACAGATAAAT ACAAGGACAT CTACACTGAG CTGAGCATCG TGCGGGCCAA AGCGGAGCGG 
GATCTGGGCC GCCTGCGGGA GCAGCTGCAG CTGGCCCACA ATGCACTGGG GGAGCCGACG 
CTGGAGGACA TGGAGCGCGG AGGATACGAT ATCATGAAGT CCAAGAGCAA CCCGGATATA 
TTAAAGATGG CGGCAGCAGC GGCAAAACGC TCCGAGAGGA CAATGAGGTC AAAGAGTCTT 
AAAGAAGGCC TGACGGCGGA GCAGAGGCTT CACTTGTTCG ACAACAAGGA CACGAAGGAC 
TTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009304639.1
CDS	673..3858
Translation	MSSAKENTCRKFQANIFNKSKCQNCFKPRELHLLTDQDLNQAKPIYGGWLCLAPEGTDFDNPMQRSRKWQRRFFVLYEHGCLRFALDESPSTLPQGTVNMNQCTDVVDAEPRTGQKNALCIVTPEQEYFIRGDNKEIINGWSEQLAVYPRTNKQNQKKKRKVEPATSQEPGPAKVAVTGAGIPDAEKTPDSSSIIWQEELQSREADAAHTWSSEIPPVGSPLPSTGEGSTVSSVNGDEVDRCGLSFHSPVSQAPVDPLSPTGSSSSRQSAETGGVPRCLSPAPSDPFPSGSSLLSNGSHISGSMSSLDSEASGSTVTSSDSHPPEPRALKRSRLRSPQTRERELVLSPERSGRSNVIEKLEALELEQQERMEVQESSSTEARQGRSQNRRFQRQEQKTDAAQALDFHPPLRRAKSLDRRTTESVMTPDLLNFKKGWMVKLDEEGQWKKYWFVLTDHSLRYYKDSIAEEASDLDGEIDLSTCYNVTEYQAQRNYGFQIYTQEAVHTLSAMTAGIRRNWIQAVMKNVRPATAPDVASLTDEHGSFEGVCRPDVTQDSPSSEASVERETGAKKSRARERRREGRSKTFDWAEFRPIAQALAQQRAQEAELTERSKRREERRRRYESVTGATFDPALMEQEGGVAQTEAPPLSAEWQQRVEDEIEQRWQQVEKTPIRDERRVPLGSAHSRDATDMEKLLEGYKRGVEELKAQLENCHQQLLDSNRHKQDLEGKLKMAVEREQQTRAGYISPATCERGFAAMEESHQKVIEELQKKHQRELEKLQEEKERLLEEETAATIAAIEAMKNAHRSELERELEKARKATSSTENADIEEIHKQHEEELVSYQREIEVLSEQYSQKCLENAHLAQALEAERQALRQCQRENQELNAHNQELNNRLAAEITKMRSMATEDGADTGGLIQGKELYELEVMLRVKESEVQYLKQEINSLKDELQTAQRDKKYATDKYKDIYTELSIVRAKAERDLGRLREQLQLAHNALGEPTLEDMERGGYDIMKSKSNPDILKMAAAAAKRSERTMRSKSLKEGLTAEQRLHLFDNKDTKDF

Target ORF information:

RefSeq Version	XM_009306364.3
Organism	Danio rerio(zebrafish)
Definition	Danio rerio myosin phosphatase Rho interacting protein (mprip), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009306364.3

ORF Insert Sequence:

ATGTCGAGCG CGAAGGAAAA CACGTGTAGG AAATTTCAGG CGAACATTTT CAACAAAAGC 
AAGTGTCAGA ACTGCTTCAA GCCGCGAGAG CTGCATCTGC TGACGGATCA GGATCTCAAT 
CAGGCCAAGC CCATCTATGG TGGATGGCTG TGTTTGGCTC CGGAGGGGAC GGATTTTGAC 
AACCCCATGC AGAGATCACG GAAATGGCAG CGGCGATTCT TCGTCCTGTA TGAGCACGGC 
TGCTTGCGCT TCGCTCTGGA TGAATCGCCC AGCACGCTGC CGCAGGGAAC GGTGAACATG 
AACCAGTGCA CAGACGTGGT GGACGCGGAG CCGCGCACGG GCCAGAAGAA CGCGCTGTGC 
ATCGTGACGC CCGAGCAGGA GTATTTCATC CGCGGAGACA ATAAAGAGAT CATCAACGGA 
TGGAGTGAGC AGCTAGCGGT GTATCCCAGA ACCAACAAGC AGAACCAGAA GAAGAAGCGC 
AAGGTGGAGC CGGCCACCTC ACAGGAGCCG GGTCCGGCTA AGGTGGCGGT GACGGGCGCC 
GGCATCCCTG ACGCAGAGAA AACTCCAGAC TCCAGCTCCA TCATCTGGCA GGAGGAGCTG 
CAGAGCAGAG AAGCAGACGC CGCTCACACC TGGAGCAGCG AGATTCCTCC TGTCGGCTCA 
CCGCTGCCCT CTACAGGCGA GGGGTCGACG GTGAGCTCAG TGAATGGGGA TGAGGTTGAC 
CGCTGCGGAC TTTCCTTCCA CTCGCCGGTC TCTCAGGCTC CTGTAGACCC GCTGTCCCCG 
ACAGGCAGCA GCTCCAGCAG GCAGAGTGCA GAGACGGGCG GGGTCCCTCG CTGCCTATCC 
CCGGCTCCCA GCGACCCCTT CCCCTCAGGC AGCAGCCTCC TGTCCAACGG CTCGCACATC 
AGCGGCTCTA TGAGCTCTCT GGACTCGGAG GCTAGCGGCA GCACCGTCAC GAGCAGCGAC 
AGCCACCCGC CGGAGCCCCG CGCCCTCAAA CGAAGCCGCC TACGGAGCCC ACAGACCCGG 
GAGCGGGAGC TAGTGCTGAG CCCTGAGAGA AGCGGCCGCT CGAATGTGAT TGAGAAGCTG 
GAGGCGCTGG AGCTGGAGCA GCAGGAGCGC ATGGAGGTTC AGGAGTCCAG CAGCACTGAG 
GCCCGACAGG GGCGGAGTCA GAACAGGAGA TTCCAGCGGC AGGAGCAGAA GACGGACGCA 
GCACAGGCTC TAGACTTCCA TCCTCCTCTA AGAAGAGCCA AATCTCTGGA CCGCAGGACC 
ACTGAGTCCG TCATGACCCC TGATCTGCTG AACTTTAAGA AAGGCTGGAT GGTGAAGCTG 
GATGAAGAAG GACAGTGGAA GAAGTATTGG TTTGTGCTGA CGGATCACAG TCTGCGATAC 
TACAAGGACT CCATCGCTGA AGAGGCGTCA GATCTGGATG GAGAGATCGA CCTCAGCACA 
TGCTATAATG TTACAGAGTA TCAGGCGCAG AGGAACTACG GCTTCCAGAT TTACACTCAG 
GAGGCGGTGC ACACACTCTC CGCCATGACT GCAGGAATCC GCCGTAACTG GATCCAGGCA 
GTGATGAAGA ATGTCCGGCC AGCCACTGCT CCAGATGTAG CCAGTCTGAC GGATGAGCAT 
GGGTCGTTTG AGGGTGTCTG TCGACCGGAC GTGACGCAGG ACTCTCCCTC CTCTGAGGCG 
TCTGTGGAGC GGGAAACAGG GGCCAAAAAA AGCCGTGCGC GGGAACGCAG GCGAGAGGGG 
CGGAGCAAAA CTTTCGACTG GGCGGAGTTT CGTCCCATCG CTCAGGCATT GGCTCAGCAG 
AGAGCGCAGG AGGCGGAGCT TACAGAGCGC AGCAAGAGGA GAGAGGAGCG AAGGCGGAGG 
TATGAGAGCG TGACCGGAGC AACCTTTGAT CCTGCACTGA TGGAGCAAGA GGGGGGCGTG 
GCTCAGACTG AAGCTCCGCC CCTGTCGGCT GAATGGCAGC AGCGAGTGGA GGATGAGATC 
GAGCAACGCT GGCAGCAGGT GGAGAAAACA CCAATCAGAG ACGAGCGCAG GGTTCCGTTA 
GGCTCCGCCC ACAGCAGAGA CGCCACAGAC ATGGAGAAGC TGCTGGAGGG ATACAAGAGA 
GGGGTGGAGG AGCTCAAGGC TCAGCTGGAG AACTGCCATC AGCAGCTTCT GGACTCCAAT 
CGGCACAAGC AGGATCTGGA GGGAAAACTG AAGATGGCGG TGGAGCGCGA GCAGCAGACG 
CGAGCCGGGT ACATCTCTCC GGCCACATGT GAGCGAGGTT TCGCCGCTAT GGAGGAGTCG 
CACCAGAAGG TGATCGAGGA GCTGCAGAAG AAACACCAGA GAGAGCTGGA GAAGCTGCAG 
GAGGAGAAGG AGAGACTGCT GGAGGAGGAG ACGGCTGCTA CCATCGCCGC TATCGAGGCG 
ATGAAGAACG CACACCGGTC AGAGCTGGAG CGAGAGCTGG AGAAAGCACG CAAAGCCACC 
AGCAGCACAG AGAACGCAGA CATCGAGGAG ATCCACAAAC AGCACGAGGA GGAGCTGGTG 
TCGTATCAGC GGGAGATCGA GGTGCTGTCA GAGCAGTATT CACAGAAGTG TCTGGAGAAC 
GCTCATCTGG CTCAGGCGCT GGAGGCCGAG CGGCAGGCGC TCAGACAGTG CCAGAGGGAG 
AACCAGGAGC TCAATGCACA CAACCAGGAG CTCAACAATC GTCTGGCTGC AGAGATCACC 
AAGATGCGCT CGATGGCGAC TGAAGACGGA GCTGACACTG GAGGACTGAT TCAAGGGAAG 
GAGCTGTACG AGCTGGAGGT GATGCTGAGG GTGAAGGAGT CAGAAGTGCA GTATCTGAAG 
CAGGAGATCA ACTCTCTGAA AGATGAGCTC CAGACGGCAC AAAGAGATAA GAAATATGCA 
ACAGATAAAT ACAAGGACAT CTACACTGAG CTGAGCATCG TGCGGGCCAA AGCGGAGCGG 
GATCTGGGCC GCCTGCGGGA GCAGCTGCAG CTGGCCCACA ATGCACTGGG GGAGCCGACG 
CTGGAGGACA TGGAGCGCGG AGGATACGAT ATCATGAAGT CCAAGAGCAA CCCGGATATA 
TTAAAGATGG CGGCAGCAGC GGCAAAACGC TCCGAGAGGA CAATGAGGTC AAAGAGTCTT 
AAAGAAGGCC TGACGGCGGA GCAGAGGCTT CACTTGTTCG ACAACAAGGA CACGAAGGAC 
TTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

mprip ( XM_009306364.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009304639.1 Danio rerio myosin phosphatase Rho interacting protein (mprip), transcript variant X6, mRNA.

Price & Availability↑

CloneID	ODa17461
Clone ID Related Accession (Same CDS sequence)	XM_009306364.3 , XM_009306364.2
Accession Version	XM_009306364.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3186bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	myosin phosphatase Rho-interacting protein isoform X4
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007123.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009306364.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGAGCG CGAAGGAAAA CACGTGTAGG AAATTTCAGG CGAACATTTT CAACAAAAGC 
AAGTGTCAGA ACTGCTTCAA GCCGCGAGAG CTGCATCTGC TGACGGATCA GGATCTCAAT 
CAGGCCAAGC CCATCTATGG TGGATGGCTG TGTTTGGCTC CGGAGGGGAC GGATTTTGAC 
AACCCCATGC AGAGATCACG GAAATGGCAG CGGCGATTCT TCGTCCTGTA TGAGCACGGC 
TGCTTGCGCT TCGCTCTGGA TGAATCGCCC AGCACGCTGC CGCAGGGAAC GGTGAACATG 
AACCAGTGCA CAGACGTGGT GGACGCGGAG CCGCGCACGG GCCAGAAGAA CGCGCTGTGC 
ATCGTGACGC CCGAGCAGGA GTATTTCATC CGCGGAGACA ATAAAGAGAT CATCAACGGA 
TGGAGTGAGC AGCTAGCGGT GTATCCCAGA ACCAACAAGC AGAACCAGAA GAAGAAGCGC 
AAGGTGGAGC CGGCCACCTC ACAGGAGCCG GGTCCGGCTA AGGTGGCGGT GACGGGCGCC 
GGCATCCCTG ACGCAGAGAA AACTCCAGAC TCCAGCTCCA TCATCTGGCA GGAGGAGCTG 
CAGAGCAGAG AAGCAGACGC CGCTCACACC TGGAGCAGCG AGATTCCTCC TGTCGGCTCA 
CCGCTGCCCT CTACAGGCGA GGGGTCGACG GTGAGCTCAG TGAATGGGGA TGAGGTTGAC 
CGCTGCGGAC TTTCCTTCCA CTCGCCGGTC TCTCAGGCTC CTGTAGACCC GCTGTCCCCG 
ACAGGCAGCA GCTCCAGCAG GCAGAGTGCA GAGACGGGCG GGGTCCCTCG CTGCCTATCC 
CCGGCTCCCA GCGACCCCTT CCCCTCAGGC AGCAGCCTCC TGTCCAACGG CTCGCACATC 
AGCGGCTCTA TGAGCTCTCT GGACTCGGAG GCTAGCGGCA GCACCGTCAC GAGCAGCGAC 
AGCCACCCGC CGGAGCCCCG CGCCCTCAAA CGAAGCCGCC TACGGAGCCC ACAGACCCGG 
GAGCGGGAGC TAGTGCTGAG CCCTGAGAGA AGCGGCCGCT CGAATGTGAT TGAGAAGCTG 
GAGGCGCTGG AGCTGGAGCA GCAGGAGCGC ATGGAGGTTC AGGAGTCCAG CAGCACTGAG 
GCCCGACAGG GGCGGAGTCA GAACAGGAGA TTCCAGCGGC AGGAGCAGAA GACGGACGCA 
GCACAGGCTC TAGACTTCCA TCCTCCTCTA AGAAGAGCCA AATCTCTGGA CCGCAGGACC 
ACTGAGTCCG TCATGACCCC TGATCTGCTG AACTTTAAGA AAGGCTGGAT GGTGAAGCTG 
GATGAAGAAG GACAGTGGAA GAAGTATTGG TTTGTGCTGA CGGATCACAG TCTGCGATAC 
TACAAGGACT CCATCGCTGA AGAGGCGTCA GATCTGGATG GAGAGATCGA CCTCAGCACA 
TGCTATAATG TTACAGAGTA TCAGGCGCAG AGGAACTACG GCTTCCAGAT TTACACTCAG 
GAGGCGGTGC ACACACTCTC CGCCATGACT GCAGGAATCC GCCGTAACTG GATCCAGGCA 
GTGATGAAGA ATGTCCGGCC AGCCACTGCT CCAGATGTAG CCAGTCTGAC GGATGAGCAT 
GGGTCGTTTG AGGGTGTCTG TCGACCGGAC GTGACGCAGG ACTCTCCCTC CTCTGAGGCG 
TCTGTGGAGC GGGAAACAGG GGCCAAAAAA AGCCGTGCGC GGGAACGCAG GCGAGAGGGG 
CGGAGCAAAA CTTTCGACTG GGCGGAGTTT CGTCCCATCG CTCAGGCATT GGCTCAGCAG 
AGAGCGCAGG AGGCGGAGCT TACAGAGCGC AGCAAGAGGA GAGAGGAGCG AAGGCGGAGG 
TATGAGAGCG TGACCGGAGC AACCTTTGAT CCTGCACTGA TGGAGCAAGA GGGGGGCGTG 
GCTCAGACTG AAGCTCCGCC CCTGTCGGCT GAATGGCAGC AGCGAGTGGA GGATGAGATC 
GAGCAACGCT GGCAGCAGGT GGAGAAAACA CCAATCAGAG ACGAGCGCAG GGTTCCGTTA 
GGCTCCGCCC ACAGCAGAGA CGCCACAGAC ATGGAGAAGC TGCTGGAGGG ATACAAGAGA 
GGGGTGGAGG AGCTCAAGGC TCAGCTGGAG AACTGCCATC AGCAGCTTCT GGACTCCAAT 
CGGCACAAGC AGGATCTGGA GGGAAAACTG AAGATGGCGG TGGAGCGCGA GCAGCAGACG 
CGAGCCGGGT ACATCTCTCC GGCCACATGT GAGCGAGGTT TCGCCGCTAT GGAGGAGTCG 
CACCAGAAGG TGATCGAGGA GCTGCAGAAG AAACACCAGA GAGAGCTGGA GAAGCTGCAG 
GAGGAGAAGG AGAGACTGCT GGAGGAGGAG ACGGCTGCTA CCATCGCCGC TATCGAGGCG 
ATGAAGAACG CACACCGGTC AGAGCTGGAG CGAGAGCTGG AGAAAGCACG CAAAGCCACC 
AGCAGCACAG AGAACGCAGA CATCGAGGAG ATCCACAAAC AGCACGAGGA GGAGCTGGTG 
TCGTATCAGC GGGAGATCGA GGTGCTGTCA GAGCAGTATT CACAGAAGTG TCTGGAGAAC 
GCTCATCTGG CTCAGGCGCT GGAGGCCGAG CGGCAGGCGC TCAGACAGTG CCAGAGGGAG 
AACCAGGAGC TCAATGCACA CAACCAGGAG CTCAACAATC GTCTGGCTGC AGAGATCACC 
AAGATGCGCT CGATGGCGAC TGAAGACGGA GCTGACACTG GAGGACTGAT TCAAGGGAAG 
GAGCTGTACG AGCTGGAGGT GATGCTGAGG GTGAAGGAGT CAGAAGTGCA GTATCTGAAG 
CAGGAGATCA ACTCTCTGAA AGATGAGCTC CAGACGGCAC AAAGAGATAA GAAATATGCA 
ACAGATAAAT ACAAGGACAT CTACACTGAG CTGAGCATCG TGCGGGCCAA AGCGGAGCGG 
GATCTGGGCC GCCTGCGGGA GCAGCTGCAG CTGGCCCACA ATGCACTGGG GGAGCCGACG 
CTGGAGGACA TGGAGCGCGG AGGATACGAT ATCATGAAGT CCAAGAGCAA CCCGGATATA 
TTAAAGATGG CGGCAGCAGC GGCAAAACGC TCCGAGAGGA CAATGAGGTC AAAGAGTCTT 
AAAGAAGGCC TGACGGCGGA GCAGAGGCTT CACTTGTTCG ACAACAAGGA CACGAAGGAC 
TTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009304639.1
CDS	702..3887
Translation	MSSAKENTCRKFQANIFNKSKCQNCFKPRELHLLTDQDLNQAKPIYGGWLCLAPEGTDFDNPMQRSRKWQRRFFVLYEHGCLRFALDESPSTLPQGTVNMNQCTDVVDAEPRTGQKNALCIVTPEQEYFIRGDNKEIINGWSEQLAVYPRTNKQNQKKKRKVEPATSQEPGPAKVAVTGAGIPDAEKTPDSSSIIWQEELQSREADAAHTWSSEIPPVGSPLPSTGEGSTVSSVNGDEVDRCGLSFHSPVSQAPVDPLSPTGSSSSRQSAETGGVPRCLSPAPSDPFPSGSSLLSNGSHISGSMSSLDSEASGSTVTSSDSHPPEPRALKRSRLRSPQTRERELVLSPERSGRSNVIEKLEALELEQQERMEVQESSSTEARQGRSQNRRFQRQEQKTDAAQALDFHPPLRRAKSLDRRTTESVMTPDLLNFKKGWMVKLDEEGQWKKYWFVLTDHSLRYYKDSIAEEASDLDGEIDLSTCYNVTEYQAQRNYGFQIYTQEAVHTLSAMTAGIRRNWIQAVMKNVRPATAPDVASLTDEHGSFEGVCRPDVTQDSPSSEASVERETGAKKSRARERRREGRSKTFDWAEFRPIAQALAQQRAQEAELTERSKRREERRRRYESVTGATFDPALMEQEGGVAQTEAPPLSAEWQQRVEDEIEQRWQQVEKTPIRDERRVPLGSAHSRDATDMEKLLEGYKRGVEELKAQLENCHQQLLDSNRHKQDLEGKLKMAVEREQQTRAGYISPATCERGFAAMEESHQKVIEELQKKHQRELEKLQEEKERLLEEETAATIAAIEAMKNAHRSELERELEKARKATSSTENADIEEIHKQHEEELVSYQREIEVLSEQYSQKCLENAHLAQALEAERQALRQCQRENQELNAHNQELNNRLAAEITKMRSMATEDGADTGGLIQGKELYELEVMLRVKESEVQYLKQEINSLKDELQTAQRDKKYATDKYKDIYTELSIVRAKAERDLGRLREQLQLAHNALGEPTLEDMERGGYDIMKSKSNPDILKMAAAAAKRSERTMRSKSLKEGLTAEQRLHLFDNKDTKDF

Target ORF information:

RefSeq Version	XM_009306364.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio myosin phosphatase Rho interacting protein (mprip), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009306364.2

ORF Insert Sequence:

ATGTCGAGCG CGAAGGAAAA CACGTGTAGG AAATTTCAGG CGAACATTTT CAACAAAAGC 
AAGTGTCAGA ACTGCTTCAA GCCGCGAGAG CTGCATCTGC TGACGGATCA GGATCTCAAT 
CAGGCCAAGC CCATCTATGG TGGATGGCTG TGTTTGGCTC CGGAGGGGAC GGATTTTGAC 
AACCCCATGC AGAGATCACG GAAATGGCAG CGGCGATTCT TCGTCCTGTA TGAGCACGGC 
TGCTTGCGCT TCGCTCTGGA TGAATCGCCC AGCACGCTGC CGCAGGGAAC GGTGAACATG 
AACCAGTGCA CAGACGTGGT GGACGCGGAG CCGCGCACGG GCCAGAAGAA CGCGCTGTGC 
ATCGTGACGC CCGAGCAGGA GTATTTCATC CGCGGAGACA ATAAAGAGAT CATCAACGGA 
TGGAGTGAGC AGCTAGCGGT GTATCCCAGA ACCAACAAGC AGAACCAGAA GAAGAAGCGC 
AAGGTGGAGC CGGCCACCTC ACAGGAGCCG GGTCCGGCTA AGGTGGCGGT GACGGGCGCC 
GGCATCCCTG ACGCAGAGAA AACTCCAGAC TCCAGCTCCA TCATCTGGCA GGAGGAGCTG 
CAGAGCAGAG AAGCAGACGC CGCTCACACC TGGAGCAGCG AGATTCCTCC TGTCGGCTCA 
CCGCTGCCCT CTACAGGCGA GGGGTCGACG GTGAGCTCAG TGAATGGGGA TGAGGTTGAC 
CGCTGCGGAC TTTCCTTCCA CTCGCCGGTC TCTCAGGCTC CTGTAGACCC GCTGTCCCCG 
ACAGGCAGCA GCTCCAGCAG GCAGAGTGCA GAGACGGGCG GGGTCCCTCG CTGCCTATCC 
CCGGCTCCCA GCGACCCCTT CCCCTCAGGC AGCAGCCTCC TGTCCAACGG CTCGCACATC 
AGCGGCTCTA TGAGCTCTCT GGACTCGGAG GCTAGCGGCA GCACCGTCAC GAGCAGCGAC 
AGCCACCCGC CGGAGCCCCG CGCCCTCAAA CGAAGCCGCC TACGGAGCCC ACAGACCCGG 
GAGCGGGAGC TAGTGCTGAG CCCTGAGAGA AGCGGCCGCT CGAATGTGAT TGAGAAGCTG 
GAGGCGCTGG AGCTGGAGCA GCAGGAGCGC ATGGAGGTTC AGGAGTCCAG CAGCACTGAG 
GCCCGACAGG GGCGGAGTCA GAACAGGAGA TTCCAGCGGC AGGAGCAGAA GACGGACGCA 
GCACAGGCTC TAGACTTCCA TCCTCCTCTA AGAAGAGCCA AATCTCTGGA CCGCAGGACC 
ACTGAGTCCG TCATGACCCC TGATCTGCTG AACTTTAAGA AAGGCTGGAT GGTGAAGCTG 
GATGAAGAAG GACAGTGGAA GAAGTATTGG TTTGTGCTGA CGGATCACAG TCTGCGATAC 
TACAAGGACT CCATCGCTGA AGAGGCGTCA GATCTGGATG GAGAGATCGA CCTCAGCACA 
TGCTATAATG TTACAGAGTA TCAGGCGCAG AGGAACTACG GCTTCCAGAT TTACACTCAG 
GAGGCGGTGC ACACACTCTC CGCCATGACT GCAGGAATCC GCCGTAACTG GATCCAGGCA 
GTGATGAAGA ATGTCCGGCC AGCCACTGCT CCAGATGTAG CCAGTCTGAC GGATGAGCAT 
GGGTCGTTTG AGGGTGTCTG TCGACCGGAC GTGACGCAGG ACTCTCCCTC CTCTGAGGCG 
TCTGTGGAGC GGGAAACAGG GGCCAAAAAA AGCCGTGCGC GGGAACGCAG GCGAGAGGGG 
CGGAGCAAAA CTTTCGACTG GGCGGAGTTT CGTCCCATCG CTCAGGCATT GGCTCAGCAG 
AGAGCGCAGG AGGCGGAGCT TACAGAGCGC AGCAAGAGGA GAGAGGAGCG AAGGCGGAGG 
TATGAGAGCG TGACCGGAGC AACCTTTGAT CCTGCACTGA TGGAGCAAGA GGGGGGCGTG 
GCTCAGACTG AAGCTCCGCC CCTGTCGGCT GAATGGCAGC AGCGAGTGGA GGATGAGATC 
GAGCAACGCT GGCAGCAGGT GGAGAAAACA CCAATCAGAG ACGAGCGCAG GGTTCCGTTA 
GGCTCCGCCC ACAGCAGAGA CGCCACAGAC ATGGAGAAGC TGCTGGAGGG ATACAAGAGA 
GGGGTGGAGG AGCTCAAGGC TCAGCTGGAG AACTGCCATC AGCAGCTTCT GGACTCCAAT 
CGGCACAAGC AGGATCTGGA GGGAAAACTG AAGATGGCGG TGGAGCGCGA GCAGCAGACG 
CGAGCCGGGT ACATCTCTCC GGCCACATGT GAGCGAGGTT TCGCCGCTAT GGAGGAGTCG 
CACCAGAAGG TGATCGAGGA GCTGCAGAAG AAACACCAGA GAGAGCTGGA GAAGCTGCAG 
GAGGAGAAGG AGAGACTGCT GGAGGAGGAG ACGGCTGCTA CCATCGCCGC TATCGAGGCG 
ATGAAGAACG CACACCGGTC AGAGCTGGAG CGAGAGCTGG AGAAAGCACG CAAAGCCACC 
AGCAGCACAG AGAACGCAGA CATCGAGGAG ATCCACAAAC AGCACGAGGA GGAGCTGGTG 
TCGTATCAGC GGGAGATCGA GGTGCTGTCA GAGCAGTATT CACAGAAGTG TCTGGAGAAC 
GCTCATCTGG CTCAGGCGCT GGAGGCCGAG CGGCAGGCGC TCAGACAGTG CCAGAGGGAG 
AACCAGGAGC TCAATGCACA CAACCAGGAG CTCAACAATC GTCTGGCTGC AGAGATCACC 
AAGATGCGCT CGATGGCGAC TGAAGACGGA GCTGACACTG GAGGACTGAT TCAAGGGAAG 
GAGCTGTACG AGCTGGAGGT GATGCTGAGG GTGAAGGAGT CAGAAGTGCA GTATCTGAAG 
CAGGAGATCA ACTCTCTGAA AGATGAGCTC CAGACGGCAC AAAGAGATAA GAAATATGCA 
ACAGATAAAT ACAAGGACAT CTACACTGAG CTGAGCATCG TGCGGGCCAA AGCGGAGCGG 
GATCTGGGCC GCCTGCGGGA GCAGCTGCAG CTGGCCCACA ATGCACTGGG GGAGCCGACG 
CTGGAGGACA TGGAGCGCGG AGGATACGAT ATCATGAAGT CCAAGAGCAA CCCGGATATA 
TTAAAGATGG CGGCAGCAGC GGCAAAACGC TCCGAGAGGA CAATGAGGTC AAAGAGTCTT 
AAAGAAGGCC TGACGGCGGA GCAGAGGCTT CACTTGTTCG ACAACAAGGA CACGAAGGAC 
TTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

mprip ( XM_009306363.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009304638.1 Danio rerio myosin phosphatase Rho interacting protein (mprip), transcript variant X5, mRNA.

Price & Availability↑

CloneID	ODa42923
Clone ID Related Accession (Same CDS sequence)	XM_009306363.2
Accession Version	XM_009306363.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3240bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	myosin phosphatase Rho-interacting protein isoform X3
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007123.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009306363.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGAGCG CGAAGGAAAA CACGTGTAGG AAATTTCAGG CGAACATTTT CAACAAAAGC 
AAGTGTCAGA ACTGCTTCAA GCCGCGAGAG CTGCATCTGC TGACGGATCA GGATCTCAAT 
CAGGCCAAGC CCATCTATGG TGGATGGCTG TGTTTGGCTC CGGAGGGGAC GGATTTTGAC 
AACCCCATGC AGAGATCACG GAAATGGCAG CGGCGATTCT TCGTCCTGTA TGAGCACGGC 
TGCTTGCGCT TCGCTCTGGA TGAATCGCCC AGCACGCTGC CGCAGGGAAC GGTGAACATG 
AACCAGTGCA CAGACGTGGT GGACGCGGAG CCGCGCACGG GCCAGAAGAA CGCGCTGTGC 
ATCGTGACGC CCGAGCAGGA GTATTTCATC CGCGGAGACA ATAAAGAGAT CATCAACGGA 
TGGAGTGAGC AGCTAGCGGT GTATCCCAGA ACCAACAAGC AGAACCAGAA GAAGAAGCGC 
AAGGTGGAGC CGGCCACCTC ACAGGAGCCG GGTCCGGCTA AGGTGGCGGT GACGGGCGCC 
GGCATCCCTG ACGCAGAGAA AACTCCAGAC TCCAGCTCCA TCATCTGGCA GGAGGAGCTG 
CAGAGCAGAG AAGCAGACGC CGCTCACACC TGGAGCAGCG AGATTCCTCC TGTCGGCTCA 
CCGCTGCCCT CTACAGGCGA GGGGTCGACG GTGAGCTCAG TGAATGGGGA TGAGGTTGAC 
CGCTGCGGAC TTTCCTTCCA CTCGCCGGTC TCTCAGGCTC CTGTAGACCC GCTGTCCCCG 
ACAGGCAGCA GCTCCAGCAG GCAGAGTGCA GAGACGGGCG GGGTCCCTCG CTGCCTATCC 
CCGGCTCCCA GCGACCCCTT CCCCTCAGGC AGCAGCCTCC TGTCCAACGG CTCGCACATC 
AGCGGCTCTA TGAGCTCTCT GGACTCGGAG GCTAGCGGCA GCACCGTCAC GAGCAGCGAC 
AGCCACCCGC CGGAGCCCCG CGCCCTCAAA CGAAGCCGCC TACGGAGCCC ACAGACCCGG 
GAGCGGGAGC TAGTGCTGAG CCCTGAGAGA AGCGGCCGCT CGAATGTGAT TGAGAAGCTG 
GAGGCGCTGG AGCTGGAGCA GCAGGAGCGC ATGGAGGTTC AGGAGTCCAG CAGCACTGAG 
GCCCGACAGG GGCGGAGTCA GAACAGGAGA TTCCAGCGGC AGGAGCAGAA GACGGACGCA 
GCACAGGCTC TAGACTTCCA TCCTCCTCTA AGAAGAGCCA AATCTCTGGA CCGCAGGACC 
ACTGAGTCCG TCATGACCCC TGATCTGCTG AACTTTAAGA AAGGCTGGAT GGTGAAGCTG 
GATGAAGAAG GACAGTGGAA GAAGTATTGG TTTGTGCTGA CGGATCACAG TCTGCGATAC 
TACAAGGACT CCATCGCTGA AGAGGCGTCA GATCTGGATG GAGAGATCGA CCTCAGCACA 
TGCTATAATG TTACAGAGTA TCAGGCGCAG AGGAACTACG GCTTCCAGAT TTACACTCAG 
GAGGCGGTGC ACACACTCTC CGCCATGACT GCAGGAATCC GCCGTAACTG GATCCAGGCA 
GTGATGAAGA ATGTCCGGCC AGCCACTGCT CCAGATGTAG CCAGTCTGAC GGATGAGCAT 
GGGTCGTTTG AGGGTGTCTG TCGACCGGAC GTGACGCAGG ACTCTCCCTC CTCTGAGGCG 
TCTGTGGAGC GGGAAACAGG GGCCAAAAAA AGCCGTGCGC GGGAACGCAG GCGAGAGGGG 
CGGAGCAAAA CTTTCGACTG GGCGGAGTTT CGTCCCATCG CTCAGGCATT GGCTCAGCAG 
AGAGCGCAGG AGGCGGAGCT TACAGAGCGC AGCAAGAGGA GAGAGGAGCG AAGGCGGAGG 
TATGAGAGCG TGACCGGAGC AACCTTTGAT CCTGCACTGA TGGAGCAAGA GGGGGGCGTG 
GCTCAGACTG AAGCTCCGCC CCTGTCGGCT GAATGGCAGC AGCGAGTGGA GGATGAGATC 
GAGCAACGCT GGCAGCAGGT GGAGAAAACA CCAATCAGAG ACGAGCGCAG GGTTCCGTTA 
GGCTCCGCCC ACAGCAGAGA CGCCACAGAC ATGGAGAAGC TGCTGGAGGG ATACAAGAGA 
GGGGTGGAGG AGCTCAAGGC TCAGCTGGAG AACTGCCATC AGCAGCTTCT GGACTCCAAT 
CGGCACAAGC AGGATCTGGA GGGAAAACTG AAGATGGCGG TGGAGCGCGA GCAGCAGACG 
CGAGCCGGGT ACATCTCTCC GCTGGAGTCC CCTTTGAGTG TTGAGACAGA GCCGCAGATG 
AAGAGGACTG AACTGGCCAC ATGTGAGCGA GGTTTCGCCG CTATGGAGGA GTCGCACCAG 
AAGGTGATCG AGGAGCTGCA GAAGAAACAC CAGAGAGAGC TGGAGAAGCT GCAGGAGGAG 
AAGGAGAGAC TGCTGGAGGA GGAGACGGCT GCTACCATCG CCGCTATCGA GGCGATGAAG 
AACGCACACC GGTCAGAGCT GGAGCGAGAG CTGGAGAAAG CACGCAAAGC CACCAGCAGC 
ACAGAGAACG CAGACATCGA GGAGATCCAC AAACAGCACG AGGAGGAGCT GGTGTCGTAT 
CAGCGGGAGA TCGAGGTGCT GTCAGAGCAG TATTCACAGA AGTGTCTGGA GAACGCTCAT 
CTGGCTCAGG CGCTGGAGGC CGAGCGGCAG GCGCTCAGAC AGTGCCAGAG GGAGAACCAG 
GAGCTCAATG CACACAACCA GGAGCTCAAC AATCGTCTGG CTGCAGAGAT CACCAAGATG 
CGCTCGATGG CGACTGAAGA CGGAGCTGAC ACTGGAGGAC TGATTCAAGG GAAGGAGCTG 
TACGAGCTGG AGGTGATGCT GAGGGTGAAG GAGTCAGAAG TGCAGTATCT GAAGCAGGAG 
ATCAACTCTC TGAAAGATGA GCTCCAGACG GCACAAAGAG ATAAGAAATA TGCAACAGAT 
AAATACAAGG ACATCTACAC TGAGCTGAGC ATCGTGCGGG CCAAAGCGGA GCGGGATCTG 
GGCCGCCTGC GGGAGCAGCT GCAGCTGGCC CACAATGCAC TGGGGGAGCC GACGCTGGAG 
GACATGGAGC GCGGAGGATA CGATATCATG AAGTCCAAGA GCAACCCGGA TATATTAAAG 
ATGGCGGCAG CAGCGGCAAA ACGCTCCGAG AGGACAATGA GGTCAAAGAG TCTTAAAGAA 
GGCCTGACGG CGGAGCAGAG GCTTCACTTG TTCGACAACA AGGACACGAA GGACTTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009304638.1
CDS	703..3942
Translation	MSSAKENTCRKFQANIFNKSKCQNCFKPRELHLLTDQDLNQAKPIYGGWLCLAPEGTDFDNPMQRSRKWQRRFFVLYEHGCLRFALDESPSTLPQGTVNMNQCTDVVDAEPRTGQKNALCIVTPEQEYFIRGDNKEIINGWSEQLAVYPRTNKQNQKKKRKVEPATSQEPGPAKVAVTGAGIPDAEKTPDSSSIIWQEELQSREADAAHTWSSEIPPVGSPLPSTGEGSTVSSVNGDEVDRCGLSFHSPVSQAPVDPLSPTGSSSSRQSAETGGVPRCLSPAPSDPFPSGSSLLSNGSHISGSMSSLDSEASGSTVTSSDSHPPEPRALKRSRLRSPQTRERELVLSPERSGRSNVIEKLEALELEQQERMEVQESSSTEARQGRSQNRRFQRQEQKTDAAQALDFHPPLRRAKSLDRRTTESVMTPDLLNFKKGWMVKLDEEGQWKKYWFVLTDHSLRYYKDSIAEEASDLDGEIDLSTCYNVTEYQAQRNYGFQIYTQEAVHTLSAMTAGIRRNWIQAVMKNVRPATAPDVASLTDEHGSFEGVCRPDVTQDSPSSEASVERETGAKKSRARERRREGRSKTFDWAEFRPIAQALAQQRAQEAELTERSKRREERRRRYESVTGATFDPALMEQEGGVAQTEAPPLSAEWQQRVEDEIEQRWQQVEKTPIRDERRVPLGSAHSRDATDMEKLLEGYKRGVEELKAQLENCHQQLLDSNRHKQDLEGKLKMAVEREQQTRAGYISPLESPLSVETEPQMKRTELATCERGFAAMEESHQKVIEELQKKHQRELEKLQEEKERLLEEETAATIAAIEAMKNAHRSELERELEKARKATSSTENADIEEIHKQHEEELVSYQREIEVLSEQYSQKCLENAHLAQALEAERQALRQCQRENQELNAHNQELNNRLAAEITKMRSMATEDGADTGGLIQGKELYELEVMLRVKESEVQYLKQEINSLKDELQTAQRDKKYATDKYKDIYTELSIVRAKAERDLGRLREQLQLAHNALGEPTLEDMERGGYDIMKSKSNPDILKMAAAAAKRSERTMRSKSLKEGLTAEQRLHLFDNKDTKDF

Target ORF information:

RefSeq Version	XM_009306363.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio myosin phosphatase Rho interacting protein (mprip), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009306363.2

ORF Insert Sequence:

ATGTCGAGCG CGAAGGAAAA CACGTGTAGG AAATTTCAGG CGAACATTTT CAACAAAAGC 
AAGTGTCAGA ACTGCTTCAA GCCGCGAGAG CTGCATCTGC TGACGGATCA GGATCTCAAT 
CAGGCCAAGC CCATCTATGG TGGATGGCTG TGTTTGGCTC CGGAGGGGAC GGATTTTGAC 
AACCCCATGC AGAGATCACG GAAATGGCAG CGGCGATTCT TCGTCCTGTA TGAGCACGGC 
TGCTTGCGCT TCGCTCTGGA TGAATCGCCC AGCACGCTGC CGCAGGGAAC GGTGAACATG 
AACCAGTGCA CAGACGTGGT GGACGCGGAG CCGCGCACGG GCCAGAAGAA CGCGCTGTGC 
ATCGTGACGC CCGAGCAGGA GTATTTCATC CGCGGAGACA ATAAAGAGAT CATCAACGGA 
TGGAGTGAGC AGCTAGCGGT GTATCCCAGA ACCAACAAGC AGAACCAGAA GAAGAAGCGC 
AAGGTGGAGC CGGCCACCTC ACAGGAGCCG GGTCCGGCTA AGGTGGCGGT GACGGGCGCC 
GGCATCCCTG ACGCAGAGAA AACTCCAGAC TCCAGCTCCA TCATCTGGCA GGAGGAGCTG 
CAGAGCAGAG AAGCAGACGC CGCTCACACC TGGAGCAGCG AGATTCCTCC TGTCGGCTCA 
CCGCTGCCCT CTACAGGCGA GGGGTCGACG GTGAGCTCAG TGAATGGGGA TGAGGTTGAC 
CGCTGCGGAC TTTCCTTCCA CTCGCCGGTC TCTCAGGCTC CTGTAGACCC GCTGTCCCCG 
ACAGGCAGCA GCTCCAGCAG GCAGAGTGCA GAGACGGGCG GGGTCCCTCG CTGCCTATCC 
CCGGCTCCCA GCGACCCCTT CCCCTCAGGC AGCAGCCTCC TGTCCAACGG CTCGCACATC 
AGCGGCTCTA TGAGCTCTCT GGACTCGGAG GCTAGCGGCA GCACCGTCAC GAGCAGCGAC 
AGCCACCCGC CGGAGCCCCG CGCCCTCAAA CGAAGCCGCC TACGGAGCCC ACAGACCCGG 
GAGCGGGAGC TAGTGCTGAG CCCTGAGAGA AGCGGCCGCT CGAATGTGAT TGAGAAGCTG 
GAGGCGCTGG AGCTGGAGCA GCAGGAGCGC ATGGAGGTTC AGGAGTCCAG CAGCACTGAG 
GCCCGACAGG GGCGGAGTCA GAACAGGAGA TTCCAGCGGC AGGAGCAGAA GACGGACGCA 
GCACAGGCTC TAGACTTCCA TCCTCCTCTA AGAAGAGCCA AATCTCTGGA CCGCAGGACC 
ACTGAGTCCG TCATGACCCC TGATCTGCTG AACTTTAAGA AAGGCTGGAT GGTGAAGCTG 
GATGAAGAAG GACAGTGGAA GAAGTATTGG TTTGTGCTGA CGGATCACAG TCTGCGATAC 
TACAAGGACT CCATCGCTGA AGAGGCGTCA GATCTGGATG GAGAGATCGA CCTCAGCACA 
TGCTATAATG TTACAGAGTA TCAGGCGCAG AGGAACTACG GCTTCCAGAT TTACACTCAG 
GAGGCGGTGC ACACACTCTC CGCCATGACT GCAGGAATCC GCCGTAACTG GATCCAGGCA 
GTGATGAAGA ATGTCCGGCC AGCCACTGCT CCAGATGTAG CCAGTCTGAC GGATGAGCAT 
GGGTCGTTTG AGGGTGTCTG TCGACCGGAC GTGACGCAGG ACTCTCCCTC CTCTGAGGCG 
TCTGTGGAGC GGGAAACAGG GGCCAAAAAA AGCCGTGCGC GGGAACGCAG GCGAGAGGGG 
CGGAGCAAAA CTTTCGACTG GGCGGAGTTT CGTCCCATCG CTCAGGCATT GGCTCAGCAG 
AGAGCGCAGG AGGCGGAGCT TACAGAGCGC AGCAAGAGGA GAGAGGAGCG AAGGCGGAGG 
TATGAGAGCG TGACCGGAGC AACCTTTGAT CCTGCACTGA TGGAGCAAGA GGGGGGCGTG 
GCTCAGACTG AAGCTCCGCC CCTGTCGGCT GAATGGCAGC AGCGAGTGGA GGATGAGATC 
GAGCAACGCT GGCAGCAGGT GGAGAAAACA CCAATCAGAG ACGAGCGCAG GGTTCCGTTA 
GGCTCCGCCC ACAGCAGAGA CGCCACAGAC ATGGAGAAGC TGCTGGAGGG ATACAAGAGA 
GGGGTGGAGG AGCTCAAGGC TCAGCTGGAG AACTGCCATC AGCAGCTTCT GGACTCCAAT 
CGGCACAAGC AGGATCTGGA GGGAAAACTG AAGATGGCGG TGGAGCGCGA GCAGCAGACG 
CGAGCCGGGT ACATCTCTCC GCTGGAGTCC CCTTTGAGTG TTGAGACAGA GCCGCAGATG 
AAGAGGACTG AACTGGCCAC ATGTGAGCGA GGTTTCGCCG CTATGGAGGA GTCGCACCAG 
AAGGTGATCG AGGAGCTGCA GAAGAAACAC CAGAGAGAGC TGGAGAAGCT GCAGGAGGAG 
AAGGAGAGAC TGCTGGAGGA GGAGACGGCT GCTACCATCG CCGCTATCGA GGCGATGAAG 
AACGCACACC GGTCAGAGCT GGAGCGAGAG CTGGAGAAAG CACGCAAAGC CACCAGCAGC 
ACAGAGAACG CAGACATCGA GGAGATCCAC AAACAGCACG AGGAGGAGCT GGTGTCGTAT 
CAGCGGGAGA TCGAGGTGCT GTCAGAGCAG TATTCACAGA AGTGTCTGGA GAACGCTCAT 
CTGGCTCAGG CGCTGGAGGC CGAGCGGCAG GCGCTCAGAC AGTGCCAGAG GGAGAACCAG 
GAGCTCAATG CACACAACCA GGAGCTCAAC AATCGTCTGG CTGCAGAGAT CACCAAGATG 
CGCTCGATGG CGACTGAAGA CGGAGCTGAC ACTGGAGGAC TGATTCAAGG GAAGGAGCTG 
TACGAGCTGG AGGTGATGCT GAGGGTGAAG GAGTCAGAAG TGCAGTATCT GAAGCAGGAG 
ATCAACTCTC TGAAAGATGA GCTCCAGACG GCACAAAGAG ATAAGAAATA TGCAACAGAT 
AAATACAAGG ACATCTACAC TGAGCTGAGC ATCGTGCGGG CCAAAGCGGA GCGGGATCTG 
GGCCGCCTGC GGGAGCAGCT GCAGCTGGCC CACAATGCAC TGGGGGAGCC GACGCTGGAG 
GACATGGAGC GCGGAGGATA CGATATCATG AAGTCCAAGA GCAACCCGGA TATATTAAAG 
ATGGCGGCAG CAGCGGCAAA ACGCTCCGAG AGGACAATGA GGTCAAAGAG TCTTAAAGAA 
GGCCTGACGG CGGAGCAGAG GCTTCACTTG TTCGACAACA AGGACACGAA GGACTTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

mprip ( XM_009306361.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009304636.1 Danio rerio myosin phosphatase Rho interacting protein (mprip), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa42921
Clone ID Related Accession (Same CDS sequence)	XM_009306361.2 , XM_009306361.3
Accession Version	XM_009306361.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5322bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	myosin phosphatase Rho-interacting protein isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007123.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009306361.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGAGCG CGAAGGAAAA CACGTGTAGG AAATTTCAGG CGAACATTTT CAACAAAAGC 
AAGTGTCAGA ACTGCTTCAA GCCGCGAGAG CTGCATCTGC TGACGGATCA GGATCTCAAT 
CAGGCCAAGC CCATCTATGG TGGATGGCTG TGTTTGGCTC CGGAGGGGAC GGATTTTGAC 
AACCCCATGC AGAGATCACG GAAATGGCAG CGGCGATTCT TCGTCCTGTA TGAGCACGGC 
TGCTTGCGCT TCGCTCTGGA TGAATCGCCC AGCACGCTGC CGCAGGGAAC GGTGAACATG 
AACCAGTGCA CAGACGTGGT GGACGCGGAG CCGCGCACGG GCCAGAAGAA CGCGCTGTGC 
ATCGTGACGC CCGAGCAGGA GTATTTCATC CGCGGAGACA ATAAAGAGAT CATCAACGGA 
TGGAGTGAGC AGCTAGCGGT GTATCCCAGA ACCAACAAGC AGAACCAGAA GAAGAAGCGC 
AAGGTGGAGC CGGCCACCTC ACAGGAGCCG GGTCCGGCTA AGGTGGCGGT GACGGGCGCC 
GGCATCCCTG ACGCAGAGAA AACTCCAGAC TCCAGCTCCA TCATCTGGCA GGAGGAGCTG 
CAGAGCAGAG AAGCAGACGC CGCTCACACC TGGAGCAGCG AGATTCCTCC TGTCGGCTCA 
CCGCTGCCCT CTACAGGCGA GGGGTCGACG GTGAGCTCAG TGAATGGGGA TGAGGTTGAC 
CGCTGCGGAC TTTCCTTCCA CTCGCCGGTC TCTCAGGCTC CTGTAGACCC GCTGTCCCCG 
ACAGGCAGCA GCTCCAGCAG GCAGAGTGCA GAGACGGGCG GGGTCCCTCG CTGCCTATCC 
CCGGCTCCCA GCGACCCCTT CCCCTCAGGC AGCAGCCTCC TGTCCAACGG CTCGCACATC 
AGCGGCTCTA TGAGCTCTCT GGACTCGGAG GCTAGCGGCA GCACCGTCAC GAGCAGCGAC 
AGCCACCCGC CGGAGCCCCG CGCCCTCAAA CGAAGCCGCC TACGGAGCCC ACAGACCCGG 
GAGCGGGAGC TAGTGCTGAG CCCTGAGAGA AGCGGCCGCT CGAATGTGAT TGAGAAGCTG 
GAGGCGCTGG AGCTGGAGCA GCAGGAGCGC ATGGAGGTTC AGGAGTCCAG CAGCACTGAG 
GCCCGACAGG GGCGGAGTCA GAACAGGAGA TTCCAGCGGC AGGAGCAGAA GACGGACGCA 
GCACAGGCTC TAGACTTCCA TCCTCCTCTA AGAAGAGCCA AATCTCTGGA CCGCAGGACC 
ACTGAGTCCG TCATGACCCC TGATCTGCTG AACTTTAAGA AAGGCTGGAT GGTGAAGCTG 
GATGAAGAAG GACAGTGGAA GAAGTATTGG TTTGTGCTGA CGGATCACAG TCTGCGATAC 
TACAAGGACT CCATCGCTGA AGAGGCGTCA GATCTGGATG GAGAGATCGA CCTCAGCACA 
TGCTATAATG TTACAGAGTA TCAGGCGCAG AGGAACTACG GCTTCCAGAT TTACACTCAG 
GAGGCGGTGC ACACACTCTC CGCCATGACT GCAGGAATCC GCCGTAACTG GATCCAGGCA 
GTGATGAAGA ATGTCCGGCC AGCCACTGCT CCAGATGTAG CCAGTCTGAC GGATGAGCAT 
GGGTCGTTTG AGGGTGTCTG TCGACCGGAC GTGACGCAGG ACTCTCCCTC CTCTGAGGCG 
TCTGTGGAGC GGGAAACAGG GGCCAAAAAA AGCCGTGCGC GGGAACGCAG GCGAGAGGGG 
CGGAGCAAAA CTTTCGACTG GGCGGAGTTT CGTCCCATCG CTCAGGCATT GGCTCAGCAG 
AGAGCGCAGG AGGCGGAGCT TACAGAGCGC AGCAAGAGGA GAGAGGAGCG AAGGCGGAGG 
TATGAGAGCG TGACCGGAGC AACCTTTGAT CCTGCACTGA TGGAGCAAGA GGGGGGCGTG 
GCTCAGACTG AAGCTCCGCC CCTGTCGGCT GAATGGCAGC AGCGAGTGGA GGATGAGATC 
GAGCAACGCT GGCAGCAGGT GGAGAAAACA CCAATCAGAG ACGAGCGCAG GGTTCCGTTA 
GGCTCCGCCC ACAGCAGAGA CGCCACAGAC ATGGAGAAGC TGCTGGAGGG ATACAAGAGA 
GGGGTGGAGG AGCTCAAGGC TCAGCTGGAG AACTGCCATC AGCAGCTTCT GGACTCCAAT 
CGGCACAAGC AGGATCTGGA GGGAAAACTG AAGATGGCGG TGGAGCGCGA GCAGCAGACG 
CGAGCCGGGT ACATCTCTCC GCTGGAGTCC CCTTTGAGTG TTGAGACAGA GCCGCAGATG 
AAGAGGACTG AACTGGTTAG TTCTCAAGCT CAGAGTTTAA CAAAGAAGTA CCAGGAAACC 
AAAGAGCTCC TGCAGCTGCA AGAACTGAAA AAGCGCGACA TGCAGGCACA GCTTGGCCTT 
TCTCTCTCAC ACTGGCCCGC AAAGGACCCT TCGCTTTCTG ACACCCAAAA ACACGAGGTC 
TGTCCCTCGA AGTGCACTCG GCGAACGCTC ACGCTTACTC TTCAGGATAG CGAGGGAACG 
ATTCGAGAGC TTGAGCACTT AATAAACACT GACGATCCTT TAACGTTGAG GGAGTTTATA 
AAGCTCGTCC AGTTGCATAG TGAACATGTT TGCGTGGTAG AATCTCCTCC AGGGATGGAG 
CAAAGTGAAC ACAGCCGCTC AGTTGGAGCG GAGTGCCAGA AACTGCTGGA AAGCTTGAAG 
AACCAGCAGG AGATGGAGAG CGAAGCTGTC AGGAGAAGTT TGGAAAAGGC TGGAGATTGC 
ATTCGAGACT ATGAAGCGCG TTTGCTCACT ATGGAGGACA TGCTAGGACG AGCTCAGCAG 
CAAAAAGCGG AGATTCTCCT GCAGTCCCCA TCGAGAGTAG AAGAGTTCAG CGCTGATATG 
AGCAGCAGAG ATCTGTCGCA GCAGGTGGGG ATTCTGGCCA ATGAAAATGT AGCGTTGAAT 
CAACGCTATC AGGAGATCGT GAACCAACTG AGAGAAGCAG ATCGAGAGAT AGACAGGCTG 
AAGGCGGAGG TGTATAGACT GCGACACAGT AAGGAAGACG ATGAAGAGTC CGCAGATAAG 
AGAGAGCTCT GCGAAAAATC CCACAAGCTT CAAGAAGCTC TGATTAAATT AGAGACGCTT 
GGTAGTAATC TGAAAGACAC TGAGAAAAGA TTGCAGCTAA AAGAAGCCAC TCTTCGTGGA 
CTTGGATTTC AGGTGGCGGA AAGTGAAGCT GAAGACGATC TCAAGTGTCA GTTGGAGATC 
CTTCAATGCA AGCTTTCTGA GAAAGAGAAG ATGTGCGAAG AACTCCAGTC GCAGAATCCA 
AGACAAGAGG ACGCGGTGAG GCTCAATCAG ATGCTTGTAG AAGCTCAGGA GGAGATCGCA 
GGGTTGAAAC GGAAGCTAGA AAGTCGAGCA GGGTTCAGTA GCGAAAGTGA GTTTATGGAA 
GAGGAAGGTG CGAGTGAGTT AAAGAGAAAG TCAGAGTCTC TAATGCTTGT GTTTGGTGCG 
TTAGTAAACG GCAGGGAGGC AGAATGCGAT GCGTTTATTG TGGATGAGGA TCACGCAGGA 
TTGATGACGG AGAGAAGGAT CTTATGCAGG ATTGTAGACG GTCTGCAGAA AGACGAGCAA 
TCTGTGAGGC AAGTGGTAGA GCGAATGATG GAGGATAATA ACATGCTGAT TCATGCAAAC 
AAGCTCTCCG ACATTCAACC AGGAGAGACG ATGCATGATG AAGATGACGC AGACATCATG 
AAGAGTCTCT CTGAAGCGGT TCAGAGCAAA GTGGCGTTAT TAAATCAGAC TTCTTTAGCG 
AGAACGGGTG ACAAACTCAC TTTGAGAAGC AGATCTGACT ACATACGTGA AGCAATCACA 
GACATAACAC ACATGTACCA AACGCTCAGG CTGAAGAAAG AGTTTGCACG TGCTGAAATG 
AGGACGCAAC AGGAATTAGC AGTGACGCGC ATTCATATCG AAGGAGAGCC GATCGACTCT 
TTGGATAAAA GCATTCAGCT GCAGGATCTG ATGGCGAGAC ATAAGAAGGA GCTTCGAGAG 
CTGAAGGAGG TTTACGAGAC GGATGTGGAA AAGCTTAAAA AGGAGTTGGA GAAAGCCGGA 
GAAACCCTGA AGATGAGATC TGAAGAGAAC GTCCGAGAGA TCGACTCGCT GACCGTGTGC 
ATGGAGAACC TGGAGAAAAA GCACGAAGCC GAAAGGCGAG AATTACAGAC ACGCTTCAGC 
CTGCAAATGG AGGAGCTCTG GGAAGCTGTC GGGTTTGAGG ATAAAGATTG GAGTTCGGGA 
TCCCTCAAAG AGCAAATCCT GAAGCTGGTG CGCCGAGAGT CTGAACTGAC CCAGCTAATG 
CATCAGAAAG ATGGAGACCT GCGTCTGAGA TACGAGAAAG ATCTGGAAAA GCTAAAGGCC 
ACATGTGAGC GAGGTTTCGC CGCTATGGAG GAGTCGCACC AGAAGGTGAT CGAGGAGCTG 
CAGAAGAAAC ACCAGAGAGA GCTGGAGAAG CTGCAGGAGG AGAAGGAGAG ACTGCTGGAG 
GAGGAGACGG CTGCTACCAT CGCCGCTATC GAGGCGATGA AGAACGCACA CCGGTCAGAG 
CTGGAGCGAG AGCTGGAGAA AGCACGCAAA GCCACCAGCA GCACAGAGAA CGCAGACATC 
GAGGAGATCC ACAAACAGCA CGAGGAGGAG CTGGTGTCGT ATCAGCGGGA GATCGAGGTG 
CTGTCAGAGC AGTATTCACA GAAGTGTCTG GAGAACGCTC ATCTGGCTCA GGCGCTGGAG 
GCCGAGCGGC AGGCGCTCAG ACAGTGCCAG AGGGAGAACC AGGAGCTCAA TGCACACAAC 
CAGGAGCTCA ACAATCGTCT GGCTGCAGAG ATCACCAAGA TGCGCTCGAT GGCGACTGAA 
GACGGAGCTG ACACTGGAGG ACTGATTCAA GGGAAGGAGC TGTACGAGCT GGAGGTGATG 
CTGAGGGTGA AGGAGTCAGA AGTGCAGTAT CTGAAGCAGG AGATCAACTC TCTGAAAGAT 
GAGCTCCAGA CGGCACAAAG AGATAAGAAA TATGCAACAG ATAAATACAA GGACATCTAC 
ACTGAGCTGA GCATCGTGCG GGCCAAAGCG GAGCGGGATC TGGGCCGCCT GCGGGAGCAG 
CTGCAGCTGG CCCACAATGC ACTGGGGGAG CCGACGCTGG AGGACATGGA GCGCGGAGGA 
TACGATATCA TGAAGTCCAA GAGCAACCCG GATATATTAA AGATGGCGGC AGCAGCGGCA 
AAACGCTCCG AGAGGACAAT GAGGTCAAAG AGTCTTAAAG AAGGCCTGAC GGCGGAGCAG 
AGGCTTCACT TGTTCGACAA CAAGGACACG AAGGACTTCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009304636.1
CDS	710..6031
Translation	MSSAKENTCRKFQANIFNKSKCQNCFKPRELHLLTDQDLNQAKPIYGGWLCLAPEGTDFDNPMQRSRKWQRRFFVLYEHGCLRFALDESPSTLPQGTVNMNQCTDVVDAEPRTGQKNALCIVTPEQEYFIRGDNKEIINGWSEQLAVYPRTNKQNQKKKRKVEPATSQEPGPAKVAVTGAGIPDAEKTPDSSSIIWQEELQSREADAAHTWSSEIPPVGSPLPSTGEGSTVSSVNGDEVDRCGLSFHSPVSQAPVDPLSPTGSSSSRQSAETGGVPRCLSPAPSDPFPSGSSLLSNGSHISGSMSSLDSEASGSTVTSSDSHPPEPRALKRSRLRSPQTRERELVLSPERSGRSNVIEKLEALELEQQERMEVQESSSTEARQGRSQNRRFQRQEQKTDAAQALDFHPPLRRAKSLDRRTTESVMTPDLLNFKKGWMVKLDEEGQWKKYWFVLTDHSLRYYKDSIAEEASDLDGEIDLSTCYNVTEYQAQRNYGFQIYTQEAVHTLSAMTAGIRRNWIQAVMKNVRPATAPDVASLTDEHGSFEGVCRPDVTQDSPSSEASVERETGAKKSRARERRREGRSKTFDWAEFRPIAQALAQQRAQEAELTERSKRREERRRRYESVTGATFDPALMEQEGGVAQTEAPPLSAEWQQRVEDEIEQRWQQVEKTPIRDERRVPLGSAHSRDATDMEKLLEGYKRGVEELKAQLENCHQQLLDSNRHKQDLEGKLKMAVEREQQTRAGYISPLESPLSVETEPQMKRTELVSSQAQSLTKKYQETKELLQLQELKKRDMQAQLGLSLSHWPAKDPSLSDTQKHEVCPSKCTRRTLTLTLQDSEGTIRELEHLINTDDPLTLREFIKLVQLHSEHVCVVESPPGMEQSEHSRSVGAECQKLLESLKNQQEMESEAVRRSLEKAGDCIRDYEARLLTMEDMLGRAQQQKAEILLQSPSRVEEFSADMSSRDLSQQVGILANENVALNQRYQEIVNQLREADREIDRLKAEVYRLRHSKEDDEESADKRELCEKSHKLQEALIKLETLGSNLKDTEKRLQLKEATLRGLGFQVAESEAEDDLKCQLEILQCKLSEKEKMCEELQSQNPRQEDAVRLNQMLVEAQEEIAGLKRKLESRAGFSSESEFMEEEGASELKRKSESLMLVFGALVNGREAECDAFIVDEDHAGLMTERRILCRIVDGLQKDEQSVRQVVERMMEDNNMLIHANKLSDIQPGETMHDEDDADIMKSLSEAVQSKVALLNQTSLARTGDKLTLRSRSDYIREAITDITHMYQTLRLKKEFARAEMRTQQELAVTRIHIEGEPIDSLDKSIQLQDLMARHKKELRELKEVYETDVEKLKKELEKAGETLKMRSEENVREIDSLTVCMENLEKKHEAERRELQTRFSLQMEELWEAVGFEDKDWSSGSLKEQILKLVRRESELTQLMHQKDGDLRLRYEKDLEKLKATCERGFAAMEESHQKVIEELQKKHQRELEKLQEEKERLLEEETAATIAAIEAMKNAHRSELERELEKARKATSSTENADIEEIHKQHEEELVSYQREIEVLSEQYSQKCLENAHLAQALEAERQALRQCQRENQELNAHNQELNNRLAAEITKMRSMATEDGADTGGLIQGKELYELEVMLRVKESEVQYLKQEINSLKDELQTAQRDKKYATDKYKDIYTELSIVRAKAERDLGRLREQLQLAHNALGEPTLEDMERGGYDIMKSKSNPDILKMAAAAAKRSERTMRSKSLKEGLTAEQRLHLFDNKDTKDF

Target ORF information:

RefSeq Version	XM_009306361.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio myosin phosphatase Rho interacting protein (mprip), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009306361.2

ORF Insert Sequence:

ATGTCGAGCG CGAAGGAAAA CACGTGTAGG AAATTTCAGG CGAACATTTT CAACAAAAGC 
AAGTGTCAGA ACTGCTTCAA GCCGCGAGAG CTGCATCTGC TGACGGATCA GGATCTCAAT 
CAGGCCAAGC CCATCTATGG TGGATGGCTG TGTTTGGCTC CGGAGGGGAC GGATTTTGAC 
AACCCCATGC AGAGATCACG GAAATGGCAG CGGCGATTCT TCGTCCTGTA TGAGCACGGC 
TGCTTGCGCT TCGCTCTGGA TGAATCGCCC AGCACGCTGC CGCAGGGAAC GGTGAACATG 
AACCAGTGCA CAGACGTGGT GGACGCGGAG CCGCGCACGG GCCAGAAGAA CGCGCTGTGC 
ATCGTGACGC CCGAGCAGGA GTATTTCATC CGCGGAGACA ATAAAGAGAT CATCAACGGA 
TGGAGTGAGC AGCTAGCGGT GTATCCCAGA ACCAACAAGC AGAACCAGAA GAAGAAGCGC 
AAGGTGGAGC CGGCCACCTC ACAGGAGCCG GGTCCGGCTA AGGTGGCGGT GACGGGCGCC 
GGCATCCCTG ACGCAGAGAA AACTCCAGAC TCCAGCTCCA TCATCTGGCA GGAGGAGCTG 
CAGAGCAGAG AAGCAGACGC CGCTCACACC TGGAGCAGCG AGATTCCTCC TGTCGGCTCA 
CCGCTGCCCT CTACAGGCGA GGGGTCGACG GTGAGCTCAG TGAATGGGGA TGAGGTTGAC 
CGCTGCGGAC TTTCCTTCCA CTCGCCGGTC TCTCAGGCTC CTGTAGACCC GCTGTCCCCG 
ACAGGCAGCA GCTCCAGCAG GCAGAGTGCA GAGACGGGCG GGGTCCCTCG CTGCCTATCC 
CCGGCTCCCA GCGACCCCTT CCCCTCAGGC AGCAGCCTCC TGTCCAACGG CTCGCACATC 
AGCGGCTCTA TGAGCTCTCT GGACTCGGAG GCTAGCGGCA GCACCGTCAC GAGCAGCGAC 
AGCCACCCGC CGGAGCCCCG CGCCCTCAAA CGAAGCCGCC TACGGAGCCC ACAGACCCGG 
GAGCGGGAGC TAGTGCTGAG CCCTGAGAGA AGCGGCCGCT CGAATGTGAT TGAGAAGCTG 
GAGGCGCTGG AGCTGGAGCA GCAGGAGCGC ATGGAGGTTC AGGAGTCCAG CAGCACTGAG 
GCCCGACAGG GGCGGAGTCA GAACAGGAGA TTCCAGCGGC AGGAGCAGAA GACGGACGCA 
GCACAGGCTC TAGACTTCCA TCCTCCTCTA AGAAGAGCCA AATCTCTGGA CCGCAGGACC 
ACTGAGTCCG TCATGACCCC TGATCTGCTG AACTTTAAGA AAGGCTGGAT GGTGAAGCTG 
GATGAAGAAG GACAGTGGAA GAAGTATTGG TTTGTGCTGA CGGATCACAG TCTGCGATAC 
TACAAGGACT CCATCGCTGA AGAGGCGTCA GATCTGGATG GAGAGATCGA CCTCAGCACA 
TGCTATAATG TTACAGAGTA TCAGGCGCAG AGGAACTACG GCTTCCAGAT TTACACTCAG 
GAGGCGGTGC ACACACTCTC CGCCATGACT GCAGGAATCC GCCGTAACTG GATCCAGGCA 
GTGATGAAGA ATGTCCGGCC AGCCACTGCT CCAGATGTAG CCAGTCTGAC GGATGAGCAT 
GGGTCGTTTG AGGGTGTCTG TCGACCGGAC GTGACGCAGG ACTCTCCCTC CTCTGAGGCG 
TCTGTGGAGC GGGAAACAGG GGCCAAAAAA AGCCGTGCGC GGGAACGCAG GCGAGAGGGG 
CGGAGCAAAA CTTTCGACTG GGCGGAGTTT CGTCCCATCG CTCAGGCATT GGCTCAGCAG 
AGAGCGCAGG AGGCGGAGCT TACAGAGCGC AGCAAGAGGA GAGAGGAGCG AAGGCGGAGG 
TATGAGAGCG TGACCGGAGC AACCTTTGAT CCTGCACTGA TGGAGCAAGA GGGGGGCGTG 
GCTCAGACTG AAGCTCCGCC CCTGTCGGCT GAATGGCAGC AGCGAGTGGA GGATGAGATC 
GAGCAACGCT GGCAGCAGGT GGAGAAAACA CCAATCAGAG ACGAGCGCAG GGTTCCGTTA 
GGCTCCGCCC ACAGCAGAGA CGCCACAGAC ATGGAGAAGC TGCTGGAGGG ATACAAGAGA 
GGGGTGGAGG AGCTCAAGGC TCAGCTGGAG AACTGCCATC AGCAGCTTCT GGACTCCAAT 
CGGCACAAGC AGGATCTGGA GGGAAAACTG AAGATGGCGG TGGAGCGCGA GCAGCAGACG 
CGAGCCGGGT ACATCTCTCC GCTGGAGTCC CCTTTGAGTG TTGAGACAGA GCCGCAGATG 
AAGAGGACTG AACTGGTTAG TTCTCAAGCT CAGAGTTTAA CAAAGAAGTA CCAGGAAACC 
AAAGAGCTCC TGCAGCTGCA AGAACTGAAA AAGCGCGACA TGCAGGCACA GCTTGGCCTT 
TCTCTCTCAC ACTGGCCCGC AAAGGACCCT TCGCTTTCTG ACACCCAAAA ACACGAGGTC 
TGTCCCTCGA AGTGCACTCG GCGAACGCTC ACGCTTACTC TTCAGGATAG CGAGGGAACG 
ATTCGAGAGC TTGAGCACTT AATAAACACT GACGATCCTT TAACGTTGAG GGAGTTTATA 
AAGCTCGTCC AGTTGCATAG TGAACATGTT TGCGTGGTAG AATCTCCTCC AGGGATGGAG 
CAAAGTGAAC ACAGCCGCTC AGTTGGAGCG GAGTGCCAGA AACTGCTGGA AAGCTTGAAG 
AACCAGCAGG AGATGGAGAG CGAAGCTGTC AGGAGAAGTT TGGAAAAGGC TGGAGATTGC 
ATTCGAGACT ATGAAGCGCG TTTGCTCACT ATGGAGGACA TGCTAGGACG AGCTCAGCAG 
CAAAAAGCGG AGATTCTCCT GCAGTCCCCA TCGAGAGTAG AAGAGTTCAG CGCTGATATG 
AGCAGCAGAG ATCTGTCGCA GCAGGTGGGG ATTCTGGCCA ATGAAAATGT AGCGTTGAAT 
CAACGCTATC AGGAGATCGT GAACCAACTG AGAGAAGCAG ATCGAGAGAT AGACAGGCTG 
AAGGCGGAGG TGTATAGACT GCGACACAGT AAGGAAGACG ATGAAGAGTC CGCAGATAAG 
AGAGAGCTCT GCGAAAAATC CCACAAGCTT CAAGAAGCTC TGATTAAATT AGAGACGCTT 
GGTAGTAATC TGAAAGACAC TGAGAAAAGA TTGCAGCTAA AAGAAGCCAC TCTTCGTGGA 
CTTGGATTTC AGGTGGCGGA AAGTGAAGCT GAAGACGATC TCAAGTGTCA GTTGGAGATC 
CTTCAATGCA AGCTTTCTGA GAAAGAGAAG ATGTGCGAAG AACTCCAGTC GCAGAATCCA 
AGACAAGAGG ACGCGGTGAG GCTCAATCAG ATGCTTGTAG AAGCTCAGGA GGAGATCGCA 
GGGTTGAAAC GGAAGCTAGA AAGTCGAGCA GGGTTCAGTA GCGAAAGTGA GTTTATGGAA 
GAGGAAGGTG CGAGTGAGTT AAAGAGAAAG TCAGAGTCTC TAATGCTTGT GTTTGGTGCG 
TTAGTAAACG GCAGGGAGGC AGAATGCGAT GCGTTTATTG TGGATGAGGA TCACGCAGGA 
TTGATGACGG AGAGAAGGAT CTTATGCAGG ATTGTAGACG GTCTGCAGAA AGACGAGCAA 
TCTGTGAGGC AAGTGGTAGA GCGAATGATG GAGGATAATA ACATGCTGAT TCATGCAAAC 
AAGCTCTCCG ACATTCAACC AGGAGAGACG ATGCATGATG AAGATGACGC AGACATCATG 
AAGAGTCTCT CTGAAGCGGT TCAGAGCAAA GTGGCGTTAT TAAATCAGAC TTCTTTAGCG 
AGAACGGGTG ACAAACTCAC TTTGAGAAGC AGATCTGACT ACATACGTGA AGCAATCACA 
GACATAACAC ACATGTACCA AACGCTCAGG CTGAAGAAAG AGTTTGCACG TGCTGAAATG 
AGGACGCAAC AGGAATTAGC AGTGACGCGC ATTCATATCG AAGGAGAGCC GATCGACTCT 
TTGGATAAAA GCATTCAGCT GCAGGATCTG ATGGCGAGAC ATAAGAAGGA GCTTCGAGAG 
CTGAAGGAGG TTTACGAGAC GGATGTGGAA AAGCTTAAAA AGGAGTTGGA GAAAGCCGGA 
GAAACCCTGA AGATGAGATC TGAAGAGAAC GTCCGAGAGA TCGACTCGCT GACCGTGTGC 
ATGGAGAACC TGGAGAAAAA GCACGAAGCC GAAAGGCGAG AATTACAGAC ACGCTTCAGC 
CTGCAAATGG AGGAGCTCTG GGAAGCTGTC GGGTTTGAGG ATAAAGATTG GAGTTCGGGA 
TCCCTCAAAG AGCAAATCCT GAAGCTGGTG CGCCGAGAGT CTGAACTGAC CCAGCTAATG 
CATCAGAAAG ATGGAGACCT GCGTCTGAGA TACGAGAAAG ATCTGGAAAA GCTAAAGGCC 
ACATGTGAGC GAGGTTTCGC CGCTATGGAG GAGTCGCACC AGAAGGTGAT CGAGGAGCTG 
CAGAAGAAAC ACCAGAGAGA GCTGGAGAAG CTGCAGGAGG AGAAGGAGAG ACTGCTGGAG 
GAGGAGACGG CTGCTACCAT CGCCGCTATC GAGGCGATGA AGAACGCACA CCGGTCAGAG 
CTGGAGCGAG AGCTGGAGAA AGCACGCAAA GCCACCAGCA GCACAGAGAA CGCAGACATC 
GAGGAGATCC ACAAACAGCA CGAGGAGGAG CTGGTGTCGT ATCAGCGGGA GATCGAGGTG 
CTGTCAGAGC AGTATTCACA GAAGTGTCTG GAGAACGCTC ATCTGGCTCA GGCGCTGGAG 
GCCGAGCGGC AGGCGCTCAG ACAGTGCCAG AGGGAGAACC AGGAGCTCAA TGCACACAAC 
CAGGAGCTCA ACAATCGTCT GGCTGCAGAG ATCACCAAGA TGCGCTCGAT GGCGACTGAA 
GACGGAGCTG ACACTGGAGG ACTGATTCAA GGGAAGGAGC TGTACGAGCT GGAGGTGATG 
CTGAGGGTGA AGGAGTCAGA AGTGCAGTAT CTGAAGCAGG AGATCAACTC TCTGAAAGAT 
GAGCTCCAGA CGGCACAAAG AGATAAGAAA TATGCAACAG ATAAATACAA GGACATCTAC 
ACTGAGCTGA GCATCGTGCG GGCCAAAGCG GAGCGGGATC TGGGCCGCCT GCGGGAGCAG 
CTGCAGCTGG CCCACAATGC ACTGGGGGAG CCGACGCTGG AGGACATGGA GCGCGGAGGA 
TACGATATCA TGAAGTCCAA GAGCAACCCG GATATATTAA AGATGGCGGC AGCAGCGGCA 
AAACGCTCCG AGAGGACAAT GAGGTCAAAG AGTCTTAAAG AAGGCCTGAC GGCGGAGCAG 
AGGCTTCACT TGTTCGACAA CAAGGACACG AAGGACTTCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

mprip ( XM_009306361.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009304636.1 Danio rerio myosin phosphatase Rho interacting protein (mprip), transcript variant X1, mRNA.

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CloneID	ODa42921
Clone ID Related Accession (Same CDS sequence)	XM_009306361.2 , XM_009306361.3
Accession Version	XM_009306361.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5322bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	myosin phosphatase Rho-interacting protein isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007123.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009306361.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGAGCG CGAAGGAAAA CACGTGTAGG AAATTTCAGG CGAACATTTT CAACAAAAGC 
AAGTGTCAGA ACTGCTTCAA GCCGCGAGAG CTGCATCTGC TGACGGATCA GGATCTCAAT 
CAGGCCAAGC CCATCTATGG TGGATGGCTG TGTTTGGCTC CGGAGGGGAC GGATTTTGAC 
AACCCCATGC AGAGATCACG GAAATGGCAG CGGCGATTCT TCGTCCTGTA TGAGCACGGC 
TGCTTGCGCT TCGCTCTGGA TGAATCGCCC AGCACGCTGC CGCAGGGAAC GGTGAACATG 
AACCAGTGCA CAGACGTGGT GGACGCGGAG CCGCGCACGG GCCAGAAGAA CGCGCTGTGC 
ATCGTGACGC CCGAGCAGGA GTATTTCATC CGCGGAGACA ATAAAGAGAT CATCAACGGA 
TGGAGTGAGC AGCTAGCGGT GTATCCCAGA ACCAACAAGC AGAACCAGAA GAAGAAGCGC 
AAGGTGGAGC CGGCCACCTC ACAGGAGCCG GGTCCGGCTA AGGTGGCGGT GACGGGCGCC 
GGCATCCCTG ACGCAGAGAA AACTCCAGAC TCCAGCTCCA TCATCTGGCA GGAGGAGCTG 
CAGAGCAGAG AAGCAGACGC CGCTCACACC TGGAGCAGCG AGATTCCTCC TGTCGGCTCA 
CCGCTGCCCT CTACAGGCGA GGGGTCGACG GTGAGCTCAG TGAATGGGGA TGAGGTTGAC 
CGCTGCGGAC TTTCCTTCCA CTCGCCGGTC TCTCAGGCTC CTGTAGACCC GCTGTCCCCG 
ACAGGCAGCA GCTCCAGCAG GCAGAGTGCA GAGACGGGCG GGGTCCCTCG CTGCCTATCC 
CCGGCTCCCA GCGACCCCTT CCCCTCAGGC AGCAGCCTCC TGTCCAACGG CTCGCACATC 
AGCGGCTCTA TGAGCTCTCT GGACTCGGAG GCTAGCGGCA GCACCGTCAC GAGCAGCGAC 
AGCCACCCGC CGGAGCCCCG CGCCCTCAAA CGAAGCCGCC TACGGAGCCC ACAGACCCGG 
GAGCGGGAGC TAGTGCTGAG CCCTGAGAGA AGCGGCCGCT CGAATGTGAT TGAGAAGCTG 
GAGGCGCTGG AGCTGGAGCA GCAGGAGCGC ATGGAGGTTC AGGAGTCCAG CAGCACTGAG 
GCCCGACAGG GGCGGAGTCA GAACAGGAGA TTCCAGCGGC AGGAGCAGAA GACGGACGCA 
GCACAGGCTC TAGACTTCCA TCCTCCTCTA AGAAGAGCCA AATCTCTGGA CCGCAGGACC 
ACTGAGTCCG TCATGACCCC TGATCTGCTG AACTTTAAGA AAGGCTGGAT GGTGAAGCTG 
GATGAAGAAG GACAGTGGAA GAAGTATTGG TTTGTGCTGA CGGATCACAG TCTGCGATAC 
TACAAGGACT CCATCGCTGA AGAGGCGTCA GATCTGGATG GAGAGATCGA CCTCAGCACA 
TGCTATAATG TTACAGAGTA TCAGGCGCAG AGGAACTACG GCTTCCAGAT TTACACTCAG 
GAGGCGGTGC ACACACTCTC CGCCATGACT GCAGGAATCC GCCGTAACTG GATCCAGGCA 
GTGATGAAGA ATGTCCGGCC AGCCACTGCT CCAGATGTAG CCAGTCTGAC GGATGAGCAT 
GGGTCGTTTG AGGGTGTCTG TCGACCGGAC GTGACGCAGG ACTCTCCCTC CTCTGAGGCG 
TCTGTGGAGC GGGAAACAGG GGCCAAAAAA AGCCGTGCGC GGGAACGCAG GCGAGAGGGG 
CGGAGCAAAA CTTTCGACTG GGCGGAGTTT CGTCCCATCG CTCAGGCATT GGCTCAGCAG 
AGAGCGCAGG AGGCGGAGCT TACAGAGCGC AGCAAGAGGA GAGAGGAGCG AAGGCGGAGG 
TATGAGAGCG TGACCGGAGC AACCTTTGAT CCTGCACTGA TGGAGCAAGA GGGGGGCGTG 
GCTCAGACTG AAGCTCCGCC CCTGTCGGCT GAATGGCAGC AGCGAGTGGA GGATGAGATC 
GAGCAACGCT GGCAGCAGGT GGAGAAAACA CCAATCAGAG ACGAGCGCAG GGTTCCGTTA 
GGCTCCGCCC ACAGCAGAGA CGCCACAGAC ATGGAGAAGC TGCTGGAGGG ATACAAGAGA 
GGGGTGGAGG AGCTCAAGGC TCAGCTGGAG AACTGCCATC AGCAGCTTCT GGACTCCAAT 
CGGCACAAGC AGGATCTGGA GGGAAAACTG AAGATGGCGG TGGAGCGCGA GCAGCAGACG 
CGAGCCGGGT ACATCTCTCC GCTGGAGTCC CCTTTGAGTG TTGAGACAGA GCCGCAGATG 
AAGAGGACTG AACTGGTTAG TTCTCAAGCT CAGAGTTTAA CAAAGAAGTA CCAGGAAACC 
AAAGAGCTCC TGCAGCTGCA AGAACTGAAA AAGCGCGACA TGCAGGCACA GCTTGGCCTT 
TCTCTCTCAC ACTGGCCCGC AAAGGACCCT TCGCTTTCTG ACACCCAAAA ACACGAGGTC 
TGTCCCTCGA AGTGCACTCG GCGAACGCTC ACGCTTACTC TTCAGGATAG CGAGGGAACG 
ATTCGAGAGC TTGAGCACTT AATAAACACT GACGATCCTT TAACGTTGAG GGAGTTTATA 
AAGCTCGTCC AGTTGCATAG TGAACATGTT TGCGTGGTAG AATCTCCTCC AGGGATGGAG 
CAAAGTGAAC ACAGCCGCTC AGTTGGAGCG GAGTGCCAGA AACTGCTGGA AAGCTTGAAG 
AACCAGCAGG AGATGGAGAG CGAAGCTGTC AGGAGAAGTT TGGAAAAGGC TGGAGATTGC 
ATTCGAGACT ATGAAGCGCG TTTGCTCACT ATGGAGGACA TGCTAGGACG AGCTCAGCAG 
CAAAAAGCGG AGATTCTCCT GCAGTCCCCA TCGAGAGTAG AAGAGTTCAG CGCTGATATG 
AGCAGCAGAG ATCTGTCGCA GCAGGTGGGG ATTCTGGCCA ATGAAAATGT AGCGTTGAAT 
CAACGCTATC AGGAGATCGT GAACCAACTG AGAGAAGCAG ATCGAGAGAT AGACAGGCTG 
AAGGCGGAGG TGTATAGACT GCGACACAGT AAGGAAGACG ATGAAGAGTC CGCAGATAAG 
AGAGAGCTCT GCGAAAAATC CCACAAGCTT CAAGAAGCTC TGATTAAATT AGAGACGCTT 
GGTAGTAATC TGAAAGACAC TGAGAAAAGA TTGCAGCTAA AAGAAGCCAC TCTTCGTGGA 
CTTGGATTTC AGGTGGCGGA AAGTGAAGCT GAAGACGATC TCAAGTGTCA GTTGGAGATC 
CTTCAATGCA AGCTTTCTGA GAAAGAGAAG ATGTGCGAAG AACTCCAGTC GCAGAATCCA 
AGACAAGAGG ACGCGGTGAG GCTCAATCAG ATGCTTGTAG AAGCTCAGGA GGAGATCGCA 
GGGTTGAAAC GGAAGCTAGA AAGTCGAGCA GGGTTCAGTA GCGAAAGTGA GTTTATGGAA 
GAGGAAGGTG CGAGTGAGTT AAAGAGAAAG TCAGAGTCTC TAATGCTTGT GTTTGGTGCG 
TTAGTAAACG GCAGGGAGGC AGAATGCGAT GCGTTTATTG TGGATGAGGA TCACGCAGGA 
TTGATGACGG AGAGAAGGAT CTTATGCAGG ATTGTAGACG GTCTGCAGAA AGACGAGCAA 
TCTGTGAGGC AAGTGGTAGA GCGAATGATG GAGGATAATA ACATGCTGAT TCATGCAAAC 
AAGCTCTCCG ACATTCAACC AGGAGAGACG ATGCATGATG AAGATGACGC AGACATCATG 
AAGAGTCTCT CTGAAGCGGT TCAGAGCAAA GTGGCGTTAT TAAATCAGAC TTCTTTAGCG 
AGAACGGGTG ACAAACTCAC TTTGAGAAGC AGATCTGACT ACATACGTGA AGCAATCACA 
GACATAACAC ACATGTACCA AACGCTCAGG CTGAAGAAAG AGTTTGCACG TGCTGAAATG 
AGGACGCAAC AGGAATTAGC AGTGACGCGC ATTCATATCG AAGGAGAGCC GATCGACTCT 
TTGGATAAAA GCATTCAGCT GCAGGATCTG ATGGCGAGAC ATAAGAAGGA GCTTCGAGAG 
CTGAAGGAGG TTTACGAGAC GGATGTGGAA AAGCTTAAAA AGGAGTTGGA GAAAGCCGGA 
GAAACCCTGA AGATGAGATC TGAAGAGAAC GTCCGAGAGA TCGACTCGCT GACCGTGTGC 
ATGGAGAACC TGGAGAAAAA GCACGAAGCC GAAAGGCGAG AATTACAGAC ACGCTTCAGC 
CTGCAAATGG AGGAGCTCTG GGAAGCTGTC GGGTTTGAGG ATAAAGATTG GAGTTCGGGA 
TCCCTCAAAG AGCAAATCCT GAAGCTGGTG CGCCGAGAGT CTGAACTGAC CCAGCTAATG 
CATCAGAAAG ATGGAGACCT GCGTCTGAGA TACGAGAAAG ATCTGGAAAA GCTAAAGGCC 
ACATGTGAGC GAGGTTTCGC CGCTATGGAG GAGTCGCACC AGAAGGTGAT CGAGGAGCTG 
CAGAAGAAAC ACCAGAGAGA GCTGGAGAAG CTGCAGGAGG AGAAGGAGAG ACTGCTGGAG 
GAGGAGACGG CTGCTACCAT CGCCGCTATC GAGGCGATGA AGAACGCACA CCGGTCAGAG 
CTGGAGCGAG AGCTGGAGAA AGCACGCAAA GCCACCAGCA GCACAGAGAA CGCAGACATC 
GAGGAGATCC ACAAACAGCA CGAGGAGGAG CTGGTGTCGT ATCAGCGGGA GATCGAGGTG 
CTGTCAGAGC AGTATTCACA GAAGTGTCTG GAGAACGCTC ATCTGGCTCA GGCGCTGGAG 
GCCGAGCGGC AGGCGCTCAG ACAGTGCCAG AGGGAGAACC AGGAGCTCAA TGCACACAAC 
CAGGAGCTCA ACAATCGTCT GGCTGCAGAG ATCACCAAGA TGCGCTCGAT GGCGACTGAA 
GACGGAGCTG ACACTGGAGG ACTGATTCAA GGGAAGGAGC TGTACGAGCT GGAGGTGATG 
CTGAGGGTGA AGGAGTCAGA AGTGCAGTAT CTGAAGCAGG AGATCAACTC TCTGAAAGAT 
GAGCTCCAGA CGGCACAAAG AGATAAGAAA TATGCAACAG ATAAATACAA GGACATCTAC 
ACTGAGCTGA GCATCGTGCG GGCCAAAGCG GAGCGGGATC TGGGCCGCCT GCGGGAGCAG 
CTGCAGCTGG CCCACAATGC ACTGGGGGAG CCGACGCTGG AGGACATGGA GCGCGGAGGA 
TACGATATCA TGAAGTCCAA GAGCAACCCG GATATATTAA AGATGGCGGC AGCAGCGGCA 
AAACGCTCCG AGAGGACAAT GAGGTCAAAG AGTCTTAAAG AAGGCCTGAC GGCGGAGCAG 
AGGCTTCACT TGTTCGACAA CAAGGACACG AAGGACTTCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009304636.1
CDS	680..6001
Translation	MSSAKENTCRKFQANIFNKSKCQNCFKPRELHLLTDQDLNQAKPIYGGWLCLAPEGTDFDNPMQRSRKWQRRFFVLYEHGCLRFALDESPSTLPQGTVNMNQCTDVVDAEPRTGQKNALCIVTPEQEYFIRGDNKEIINGWSEQLAVYPRTNKQNQKKKRKVEPATSQEPGPAKVAVTGAGIPDAEKTPDSSSIIWQEELQSREADAAHTWSSEIPPVGSPLPSTGEGSTVSSVNGDEVDRCGLSFHSPVSQAPVDPLSPTGSSSSRQSAETGGVPRCLSPAPSDPFPSGSSLLSNGSHISGSMSSLDSEASGSTVTSSDSHPPEPRALKRSRLRSPQTRERELVLSPERSGRSNVIEKLEALELEQQERMEVQESSSTEARQGRSQNRRFQRQEQKTDAAQALDFHPPLRRAKSLDRRTTESVMTPDLLNFKKGWMVKLDEEGQWKKYWFVLTDHSLRYYKDSIAEEASDLDGEIDLSTCYNVTEYQAQRNYGFQIYTQEAVHTLSAMTAGIRRNWIQAVMKNVRPATAPDVASLTDEHGSFEGVCRPDVTQDSPSSEASVERETGAKKSRARERRREGRSKTFDWAEFRPIAQALAQQRAQEAELTERSKRREERRRRYESVTGATFDPALMEQEGGVAQTEAPPLSAEWQQRVEDEIEQRWQQVEKTPIRDERRVPLGSAHSRDATDMEKLLEGYKRGVEELKAQLENCHQQLLDSNRHKQDLEGKLKMAVEREQQTRAGYISPLESPLSVETEPQMKRTELVSSQAQSLTKKYQETKELLQLQELKKRDMQAQLGLSLSHWPAKDPSLSDTQKHEVCPSKCTRRTLTLTLQDSEGTIRELEHLINTDDPLTLREFIKLVQLHSEHVCVVESPPGMEQSEHSRSVGAECQKLLESLKNQQEMESEAVRRSLEKAGDCIRDYEARLLTMEDMLGRAQQQKAEILLQSPSRVEEFSADMSSRDLSQQVGILANENVALNQRYQEIVNQLREADREIDRLKAEVYRLRHSKEDDEESADKRELCEKSHKLQEALIKLETLGSNLKDTEKRLQLKEATLRGLGFQVAESEAEDDLKCQLEILQCKLSEKEKMCEELQSQNPRQEDAVRLNQMLVEAQEEIAGLKRKLESRAGFSSESEFMEEEGASELKRKSESLMLVFGALVNGREAECDAFIVDEDHAGLMTERRILCRIVDGLQKDEQSVRQVVERMMEDNNMLIHANKLSDIQPGETMHDEDDADIMKSLSEAVQSKVALLNQTSLARTGDKLTLRSRSDYIREAITDITHMYQTLRLKKEFARAEMRTQQELAVTRIHIEGEPIDSLDKSIQLQDLMARHKKELRELKEVYETDVEKLKKELEKAGETLKMRSEENVREIDSLTVCMENLEKKHEAERRELQTRFSLQMEELWEAVGFEDKDWSSGSLKEQILKLVRRESELTQLMHQKDGDLRLRYEKDLEKLKATCERGFAAMEESHQKVIEELQKKHQRELEKLQEEKERLLEEETAATIAAIEAMKNAHRSELERELEKARKATSSTENADIEEIHKQHEEELVSYQREIEVLSEQYSQKCLENAHLAQALEAERQALRQCQRENQELNAHNQELNNRLAAEITKMRSMATEDGADTGGLIQGKELYELEVMLRVKESEVQYLKQEINSLKDELQTAQRDKKYATDKYKDIYTELSIVRAKAERDLGRLREQLQLAHNALGEPTLEDMERGGYDIMKSKSNPDILKMAAAAAKRSERTMRSKSLKEGLTAEQRLHLFDNKDTKDF

Target ORF information:

RefSeq Version	XM_009306361.3
Organism	Danio rerio(zebrafish)
Definition	Danio rerio myosin phosphatase Rho interacting protein (mprip), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009306361.3

ORF Insert Sequence:

ATGTCGAGCG CGAAGGAAAA CACGTGTAGG AAATTTCAGG CGAACATTTT CAACAAAAGC 
AAGTGTCAGA ACTGCTTCAA GCCGCGAGAG CTGCATCTGC TGACGGATCA GGATCTCAAT 
CAGGCCAAGC CCATCTATGG TGGATGGCTG TGTTTGGCTC CGGAGGGGAC GGATTTTGAC 
AACCCCATGC AGAGATCACG GAAATGGCAG CGGCGATTCT TCGTCCTGTA TGAGCACGGC 
TGCTTGCGCT TCGCTCTGGA TGAATCGCCC AGCACGCTGC CGCAGGGAAC GGTGAACATG 
AACCAGTGCA CAGACGTGGT GGACGCGGAG CCGCGCACGG GCCAGAAGAA CGCGCTGTGC 
ATCGTGACGC CCGAGCAGGA GTATTTCATC CGCGGAGACA ATAAAGAGAT CATCAACGGA 
TGGAGTGAGC AGCTAGCGGT GTATCCCAGA ACCAACAAGC AGAACCAGAA GAAGAAGCGC 
AAGGTGGAGC CGGCCACCTC ACAGGAGCCG GGTCCGGCTA AGGTGGCGGT GACGGGCGCC 
GGCATCCCTG ACGCAGAGAA AACTCCAGAC TCCAGCTCCA TCATCTGGCA GGAGGAGCTG 
CAGAGCAGAG AAGCAGACGC CGCTCACACC TGGAGCAGCG AGATTCCTCC TGTCGGCTCA 
CCGCTGCCCT CTACAGGCGA GGGGTCGACG GTGAGCTCAG TGAATGGGGA TGAGGTTGAC 
CGCTGCGGAC TTTCCTTCCA CTCGCCGGTC TCTCAGGCTC CTGTAGACCC GCTGTCCCCG 
ACAGGCAGCA GCTCCAGCAG GCAGAGTGCA GAGACGGGCG GGGTCCCTCG CTGCCTATCC 
CCGGCTCCCA GCGACCCCTT CCCCTCAGGC AGCAGCCTCC TGTCCAACGG CTCGCACATC 
AGCGGCTCTA TGAGCTCTCT GGACTCGGAG GCTAGCGGCA GCACCGTCAC GAGCAGCGAC 
AGCCACCCGC CGGAGCCCCG CGCCCTCAAA CGAAGCCGCC TACGGAGCCC ACAGACCCGG 
GAGCGGGAGC TAGTGCTGAG CCCTGAGAGA AGCGGCCGCT CGAATGTGAT TGAGAAGCTG 
GAGGCGCTGG AGCTGGAGCA GCAGGAGCGC ATGGAGGTTC AGGAGTCCAG CAGCACTGAG 
GCCCGACAGG GGCGGAGTCA GAACAGGAGA TTCCAGCGGC AGGAGCAGAA GACGGACGCA 
GCACAGGCTC TAGACTTCCA TCCTCCTCTA AGAAGAGCCA AATCTCTGGA CCGCAGGACC 
ACTGAGTCCG TCATGACCCC TGATCTGCTG AACTTTAAGA AAGGCTGGAT GGTGAAGCTG 
GATGAAGAAG GACAGTGGAA GAAGTATTGG TTTGTGCTGA CGGATCACAG TCTGCGATAC 
TACAAGGACT CCATCGCTGA AGAGGCGTCA GATCTGGATG GAGAGATCGA CCTCAGCACA 
TGCTATAATG TTACAGAGTA TCAGGCGCAG AGGAACTACG GCTTCCAGAT TTACACTCAG 
GAGGCGGTGC ACACACTCTC CGCCATGACT GCAGGAATCC GCCGTAACTG GATCCAGGCA 
GTGATGAAGA ATGTCCGGCC AGCCACTGCT CCAGATGTAG CCAGTCTGAC GGATGAGCAT 
GGGTCGTTTG AGGGTGTCTG TCGACCGGAC GTGACGCAGG ACTCTCCCTC CTCTGAGGCG 
TCTGTGGAGC GGGAAACAGG GGCCAAAAAA AGCCGTGCGC GGGAACGCAG GCGAGAGGGG 
CGGAGCAAAA CTTTCGACTG GGCGGAGTTT CGTCCCATCG CTCAGGCATT GGCTCAGCAG 
AGAGCGCAGG AGGCGGAGCT TACAGAGCGC AGCAAGAGGA GAGAGGAGCG AAGGCGGAGG 
TATGAGAGCG TGACCGGAGC AACCTTTGAT CCTGCACTGA TGGAGCAAGA GGGGGGCGTG 
GCTCAGACTG AAGCTCCGCC CCTGTCGGCT GAATGGCAGC AGCGAGTGGA GGATGAGATC 
GAGCAACGCT GGCAGCAGGT GGAGAAAACA CCAATCAGAG ACGAGCGCAG GGTTCCGTTA 
GGCTCCGCCC ACAGCAGAGA CGCCACAGAC ATGGAGAAGC TGCTGGAGGG ATACAAGAGA 
GGGGTGGAGG AGCTCAAGGC TCAGCTGGAG AACTGCCATC AGCAGCTTCT GGACTCCAAT 
CGGCACAAGC AGGATCTGGA GGGAAAACTG AAGATGGCGG TGGAGCGCGA GCAGCAGACG 
CGAGCCGGGT ACATCTCTCC GCTGGAGTCC CCTTTGAGTG TTGAGACAGA GCCGCAGATG 
AAGAGGACTG AACTGGTTAG TTCTCAAGCT CAGAGTTTAA CAAAGAAGTA CCAGGAAACC 
AAAGAGCTCC TGCAGCTGCA AGAACTGAAA AAGCGCGACA TGCAGGCACA GCTTGGCCTT 
TCTCTCTCAC ACTGGCCCGC AAAGGACCCT TCGCTTTCTG ACACCCAAAA ACACGAGGTC 
TGTCCCTCGA AGTGCACTCG GCGAACGCTC ACGCTTACTC TTCAGGATAG CGAGGGAACG 
ATTCGAGAGC TTGAGCACTT AATAAACACT GACGATCCTT TAACGTTGAG GGAGTTTATA 
AAGCTCGTCC AGTTGCATAG TGAACATGTT TGCGTGGTAG AATCTCCTCC AGGGATGGAG 
CAAAGTGAAC ACAGCCGCTC AGTTGGAGCG GAGTGCCAGA AACTGCTGGA AAGCTTGAAG 
AACCAGCAGG AGATGGAGAG CGAAGCTGTC AGGAGAAGTT TGGAAAAGGC TGGAGATTGC 
ATTCGAGACT ATGAAGCGCG TTTGCTCACT ATGGAGGACA TGCTAGGACG AGCTCAGCAG 
CAAAAAGCGG AGATTCTCCT GCAGTCCCCA TCGAGAGTAG AAGAGTTCAG CGCTGATATG 
AGCAGCAGAG ATCTGTCGCA GCAGGTGGGG ATTCTGGCCA ATGAAAATGT AGCGTTGAAT 
CAACGCTATC AGGAGATCGT GAACCAACTG AGAGAAGCAG ATCGAGAGAT AGACAGGCTG 
AAGGCGGAGG TGTATAGACT GCGACACAGT AAGGAAGACG ATGAAGAGTC CGCAGATAAG 
AGAGAGCTCT GCGAAAAATC CCACAAGCTT CAAGAAGCTC TGATTAAATT AGAGACGCTT 
GGTAGTAATC TGAAAGACAC TGAGAAAAGA TTGCAGCTAA AAGAAGCCAC TCTTCGTGGA 
CTTGGATTTC AGGTGGCGGA AAGTGAAGCT GAAGACGATC TCAAGTGTCA GTTGGAGATC 
CTTCAATGCA AGCTTTCTGA GAAAGAGAAG ATGTGCGAAG AACTCCAGTC GCAGAATCCA 
AGACAAGAGG ACGCGGTGAG GCTCAATCAG ATGCTTGTAG AAGCTCAGGA GGAGATCGCA 
GGGTTGAAAC GGAAGCTAGA AAGTCGAGCA GGGTTCAGTA GCGAAAGTGA GTTTATGGAA 
GAGGAAGGTG CGAGTGAGTT AAAGAGAAAG TCAGAGTCTC TAATGCTTGT GTTTGGTGCG 
TTAGTAAACG GCAGGGAGGC AGAATGCGAT GCGTTTATTG TGGATGAGGA TCACGCAGGA 
TTGATGACGG AGAGAAGGAT CTTATGCAGG ATTGTAGACG GTCTGCAGAA AGACGAGCAA 
TCTGTGAGGC AAGTGGTAGA GCGAATGATG GAGGATAATA ACATGCTGAT TCATGCAAAC 
AAGCTCTCCG ACATTCAACC AGGAGAGACG ATGCATGATG AAGATGACGC AGACATCATG 
AAGAGTCTCT CTGAAGCGGT TCAGAGCAAA GTGGCGTTAT TAAATCAGAC TTCTTTAGCG 
AGAACGGGTG ACAAACTCAC TTTGAGAAGC AGATCTGACT ACATACGTGA AGCAATCACA 
GACATAACAC ACATGTACCA AACGCTCAGG CTGAAGAAAG AGTTTGCACG TGCTGAAATG 
AGGACGCAAC AGGAATTAGC AGTGACGCGC ATTCATATCG AAGGAGAGCC GATCGACTCT 
TTGGATAAAA GCATTCAGCT GCAGGATCTG ATGGCGAGAC ATAAGAAGGA GCTTCGAGAG 
CTGAAGGAGG TTTACGAGAC GGATGTGGAA AAGCTTAAAA AGGAGTTGGA GAAAGCCGGA 
GAAACCCTGA AGATGAGATC TGAAGAGAAC GTCCGAGAGA TCGACTCGCT GACCGTGTGC 
ATGGAGAACC TGGAGAAAAA GCACGAAGCC GAAAGGCGAG AATTACAGAC ACGCTTCAGC 
CTGCAAATGG AGGAGCTCTG GGAAGCTGTC GGGTTTGAGG ATAAAGATTG GAGTTCGGGA 
TCCCTCAAAG AGCAAATCCT GAAGCTGGTG CGCCGAGAGT CTGAACTGAC CCAGCTAATG 
CATCAGAAAG ATGGAGACCT GCGTCTGAGA TACGAGAAAG ATCTGGAAAA GCTAAAGGCC 
ACATGTGAGC GAGGTTTCGC CGCTATGGAG GAGTCGCACC AGAAGGTGAT CGAGGAGCTG 
CAGAAGAAAC ACCAGAGAGA GCTGGAGAAG CTGCAGGAGG AGAAGGAGAG ACTGCTGGAG 
GAGGAGACGG CTGCTACCAT CGCCGCTATC GAGGCGATGA AGAACGCACA CCGGTCAGAG 
CTGGAGCGAG AGCTGGAGAA AGCACGCAAA GCCACCAGCA GCACAGAGAA CGCAGACATC 
GAGGAGATCC ACAAACAGCA CGAGGAGGAG CTGGTGTCGT ATCAGCGGGA GATCGAGGTG 
CTGTCAGAGC AGTATTCACA GAAGTGTCTG GAGAACGCTC ATCTGGCTCA GGCGCTGGAG 
GCCGAGCGGC AGGCGCTCAG ACAGTGCCAG AGGGAGAACC AGGAGCTCAA TGCACACAAC 
CAGGAGCTCA ACAATCGTCT GGCTGCAGAG ATCACCAAGA TGCGCTCGAT GGCGACTGAA 
GACGGAGCTG ACACTGGAGG ACTGATTCAA GGGAAGGAGC TGTACGAGCT GGAGGTGATG 
CTGAGGGTGA AGGAGTCAGA AGTGCAGTAT CTGAAGCAGG AGATCAACTC TCTGAAAGAT 
GAGCTCCAGA CGGCACAAAG AGATAAGAAA TATGCAACAG ATAAATACAA GGACATCTAC 
ACTGAGCTGA GCATCGTGCG GGCCAAAGCG GAGCGGGATC TGGGCCGCCT GCGGGAGCAG 
CTGCAGCTGG CCCACAATGC ACTGGGGGAG CCGACGCTGG AGGACATGGA GCGCGGAGGA 
TACGATATCA TGAAGTCCAA GAGCAACCCG GATATATTAA AGATGGCGGC AGCAGCGGCA 
AAACGCTCCG AGAGGACAAT GAGGTCAAAG AGTCTTAAAG AAGGCCTGAC GGCGGAGCAG 
AGGCTTCACT TGTTCGACAA CAAGGACACG AAGGACTTCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

mprip ( XM_009306362.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009304637.1 Danio rerio myosin phosphatase Rho interacting protein (mprip), transcript variant X4, mRNA.

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CloneID	ODa42922
Clone ID Related Accession (Same CDS sequence)	XM_009306362.2 , XM_021480198.1 , XM_021480197.1
Accession Version	XM_009306362.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5283bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	myosin phosphatase Rho-interacting protein isoform X2
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007123.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009306362.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGAGCG CGAAGGAAAA CACGTGTAGG AAATTTCAGG CGAACATTTT CAACAAAAGC 
AAGTGTCAGA ACTGCTTCAA GCCGCGAGAG CTGCATCTGC TGACGGATCA GGATCTCAAT 
CAGGCCAAGC CCATCTATGG TGGATGGCTG TGTTTGGCTC CGGAGGGGAC GGATTTTGAC 
AACCCCATGC AGAGATCACG GAAATGGCAG CGGCGATTCT TCGTCCTGTA TGAGCACGGC 
TGCTTGCGCT TCGCTCTGGA TGAATCGCCC AGCACGCTGC CGCAGGGAAC GGTGAACATG 
AACCAGTGCA CAGACGTGGT GGACGCGGAG CCGCGCACGG GCCAGAAGAA CGCGCTGTGC 
ATCGTGACGC CCGAGCAGGA GTATTTCATC CGCGGAGACA ATAAAGAGAT CATCAACGGA 
TGGAGTGAGC AGCTAGCGGT GTATCCCAGA ACCAACAAGC AGAACCAGAA GAAGAAGCGC 
AAGGTGGAGC CGGCCACCTC ACAGGAGCCG GGTCCGGCTA AGGTGGCGGT GACGGGCGCC 
GGCATCCCTG ACGCAGAGAA AACTCCAGAC TCCAGCTCCA TCATCTGGCA GGAGGAGCTG 
CAGAGCAGAG AAGCAGACGC CGCTCACACC TGGAGCAGCG AGATTCCTCC TGTCGGCTCA 
CCGCTGCCCT CTACAGGCGA GGGGTCGACG GTGAGCTCAG TGAATGGGGA TGAGGTTGAC 
CGCTGCGGAC TTTCCTTCCA CTCGCCGGTC TCTCAGGCTC CTGTAGACCC GCTGTCCCCG 
ACAGGCAGCA GCTCCAGCAG GCAGAGTGCA GAGACGGGCG GGGTCCCTCG CTGCCTATCC 
CCGGCTCCCA GCGACCCCTT CCCCTCAGGC AGCAGCCTCC TGTCCAACGG CTCGCACATC 
AGCGGCTCTA TGAGCTCTCT GGACTCGGAG GCTAGCGGCA GCACCGTCAC GAGCAGCGAC 
AGCCACCCGC CGGAGCCCCG CGCCCTCAAA CGAAGCCGCC TACGGAGCCC ACAGACCCGG 
GAGCGGGAGC TAGTGCTGAG CCCTGAGAGA AGCGGCCGCT CGAATGTGAT TGAGAAGCTG 
GAGGCGCTGG AGCTGGAGCA GCAGGAGCGC ATGGAGGTTC AGGAGTCCAG CAGCACTGAG 
GCCCGACAGG GGCGGAGTCA GAACAGGAGA TTCCAGCGGC AGGAGCAGAA GACGGACGCA 
GCACAGGCTC TAGACTTCCA TCCTCCTCTA AGAAGAGCCA AATCTCTGGA CCGCAGGACC 
ACTGAGTCCG TCATGACCCC TGATCTGCTG AACTTTAAGA AAGGCTGGAT GGTGAAGCTG 
GATGAAGAAG GACAGTGGAA GAAGTATTGG TTTGTGCTGA CGGATCACAG TCTGCGATAC 
TACAAGGACT CCATCGCTGA AGAGGCGTCA GATCTGGATG GAGAGATCGA CCTCAGCACA 
TGCTATAATG TTACAGAGTA TCAGGCGCAG AGGAACTACG GCTTCCAGAT TTACACTCAG 
GAGGCGGTGC ACACACTCTC CGCCATGACT GCAGGAATCC GCCGTAACTG GATCCAGGCA 
GTGATGAAGA ATGTCCGGCC AGCCACTGCT CCAGATGTAG CCAGTCTGAC GGATGAGCAT 
GGGTCGTTTG AGGGTGTCTG TCGACCGGAC GTGACGCAGG ACTCTCCCTC CTCTGAGGCG 
TCTGTGGAGC GGGAAACAGG GGCCAAAAAA AGCCGTGCGC GGGAACGCAG GCGAGAGGGG 
CGGAGCAAAA CTTTCGACTG GGCGGAGTTT CGTCCCATCG CTCAGGCATT GGCTCAGCAG 
AGAGCGCAGG AGGCGGAGCT TACAGAGCGC AGCAAGAGGA GAGAGGAGCG AAGGCGGAGG 
TATGAGAGCG TGACCGGAGC AACCTTTGAT CCTGCACTGA TGGAGCAAGA GGGGGGCGTG 
GCTCAGACTG AAGCTCCGCC CCTGTCGGCT GAATGGCAGC AGCGAGTGGA GGATGAGATC 
GAGCAACGCT GGCAGCAGGT GGAGAAAACA CCAATCAGAG ACGAGCGCAG GGTTCCGTTA 
GGCTCCGCCC ACAGCAGAGA CGCCACAGAC ATGGAGAAGC TGCTGGAGGG ATACAAGAGA 
GGGGTGGAGG AGCTCAAGGC TCAGCTGGAG AACTGCCATC AGCAGCTTCT GGACTCCAAT 
CGGCACAAGC AGGATCTGGA GGGAAAACTG AAGATGGCGG TGGAGCGCGA GCAGCAGACG 
CGAGCCGGGT ACATCTCTCC GCTGGAGTCC CCTTTGAGTG TTGAGACAGA GCCGCAGATG 
AAGAGGACTG AACTGGTTAG TTCTCAAGCT CAGAGTTTAA CAAAGAAGTA CCAGGAAACC 
AAAGAGCTCC TGCAGCTGCA AGAACTGAAA AAGCGCGACA TGCAGGCACA GCTTGGCCTT 
TCTCTCTCAC ACTGGCCCGC AAAGGACCCT TCGCTTTCTG ACACCCAAAA ACACGAGGTC 
TGTCCCTCGA AGTGCACTCG GCGAACGCTC ACGCTTACTC TTCAGGATAG CGAGGGAACG 
ATTCGAGAGC TTGAGCACTT AATAAACACT GACGATCCTT TAACGTTGAG GGAGTTTATA 
AAGCTCGTCC AGTTGCATAG TGAACATGTT TGCGTGGTAG AATCTCCTCC AGGGATGGAG 
CAAAGTGAAC ACAGCCGCTC AGTTGGAGCG GAGTGCCAGA AACTGCTGGA AAGCTTGAAG 
AACCAGCAGG AGATGGAGAG CGAAGCTGTC AGGAGAAGTT TGGAAAAGGC TGGAGATTGC 
ATTCGAGACT ATGAAGCGCG TTTGCTCACT ATGGAGGACA TGCTAGGACG AGCTCAGCAG 
CAAAAAGCGG AGATTCTCCT GCAGTCCCCA TCGAGAGTAG AAGAGTTCAG CGCTGATATG 
AGCAGCAGAG ATCTGTCGCA GCAGGTGGGG ATTCTGGCCA ATGAAAATGT AGCGTTGAAT 
CAACGCTATC AGGAGATCGT GAACCAACTG AGAGAAGCAG ATCGAGAGAT AGACAGGCTG 
AAGGCGGAGG TGTATAGACT GCGACACAGT AAGGAAGACG ATGAAGAGTC CGCAGATAAG 
AGAGAGCTCT GCGAAAAATC CCACAAGCTT CAAGAAGCTC TGATTAAATT AGAGACGCTT 
GGTAGTAATC TGAAAGACAC TGAGAAAAGA TTGCAGCTAA AAGAAGCCAC TCTTCGTGGA 
CTTGGATTTC AGGTGGCGGA AAGTGAAGCT GAAGACGATC TCAAGTGTCA GTTGGAGATC 
CTTCAATGCA AGCTTTCTGA GAAAGAGAAG ATGTGCGAAG AACTCCAGTC GCAGAATCCA 
AGACAAGAGG ACGCGGTGAG GCTCAATCAG ATGCTTGTAG AAGCTCAGGA GGAGATCGCA 
GGGTTGAAAC GGAAGCTAGA AAGTCGAGCA GGGTTCAGTA GCGAAAGTGA GTTTATGGAA 
GAGGAAGGTG CGAGTGAGTT AAAGAGAAAG TCAGAGTCTC TAATGCTTGT GTTTGGTGCG 
TTAGTAAACG GCAGGGAGGC AGAATGCGAT GCGTTTATTG TGGATGAGGA TCACGCAGGA 
TTGATGACGG AGAGAAGGAT CTTATGCAGG ATTGTAGACG GTCTGCAGAA AGACGAGCAA 
TCTGTGAGGC AAGTGGTAGA GCGAATGATG GAGGATAATA ACATGCTGAT TCATGCAAAC 
AAGCTCTCCG ACATTCAACC AGGAGAGACG ATGCATGATG AAGATGACGC AGACATCATG 
AAGAGTCTCT CTGAAGCGGT TCAGAGCAAA GTGGCGTTAT TAAATCAGAC TTCTTTAGCG 
AGAACGGGTG ACAAACTCAC TTTGAGAAGC AGATCTGACT ACATACGTGA AGCAATCACA 
GACATAACAC ACATGTACCA AACGCTCAGG CTGAAGAAAG AGTTTGCACG TGCTGAAATG 
AGGACGCAAC AGGAATTAGC AGTGACGCGC ATTCATATCG AAGGAGAGCC GATCGACTCT 
TTGGATAAAA GCATTCAGCT GCAGGATCTG ATGGCGAGAC ATAAGAAGGA GCTTCGAGAG 
CTGAAGGAGG TTTACGAGAC GGATGTGGAA AAGCTTAAAA AGGAGTTGGA GAAAGCCGGA 
GAAACCCTGA AGATGAGATC TGAAGAGAAC GTCCGAGAGA TCGACTCGCT GACCGTGTGC 
ATGGAGAACC TGGAGAAAAA GCACGAAGCC GAAAGGCGAG AATTACAGAC ACGCTTCAGC 
CTGCAAATGG AGGAGCTCTG GGAAGCTGTC GGGTTTGAGG ATAAAGATTG GAGTTCGGGA 
TCCCTCAAAG AGCAAATCCT GAAGCTGGTG CGCCGAGAGT CTGAACTGAC CCAGCTAATG 
CATCAGAAAG ATGGAGACCT GCGTCTGAGA TACGAGAAAG ATCTGGAAAA GCTAAAGGCC 
ACATGTGAGC GAGGTTTCGC CGCTATGGAG GAGTCGCACC AGAAGGTGAT CGAGGAGCTG 
CAGAAGAAAC ACCAGAGAGA GCTGGAGAAG CTGCAGGAGG AGAAGGAGAG ACTGCTGGAG 
GAGGAGACGG CTGCTACCAT CGCCGCTATC GAGGCGATGA AGAACGCACA CCGGTCAGAG 
CTGGAGCGAG AGCTGGAGAA AGCACGCAAA GCCACCAGCA GCACAGAGAA CGCAGACATC 
GAGGAGATCC ACAAACAGCA CGAGGAGGAG CTGGTGTCGT ATCAGCGGGA GATCGAGGTG 
CTGTCAGAGC AGTATTCACA GAAGTGTCTG GAGAACGCTC ATCTGGCTCA GGCGCTGGAG 
GCCGAGCGGC AGGCGCTCAG ACAGTGCCAG AGGGAGAACC AGGAGCTCAA TGCACACAAC 
CAGGAGCTCA ACAATCGTCT GGCTGCAGAG ATCACCAAGA TGCGCTCGAT GGCGACTGAA 
GACGGAGCTG ACACTGGAGG ACTGATTCAA GGGAAGGAGC TGTACGAGCT GGAGGTGATG 
CTGAGGGTGA AGGAGTCAGA AGTGCAGTAT CTGAAGCAGG AGATCAACTC TCTGAAAGAT 
GAGCTCCAGA CGGCACAAAG AGATAAGAAA TATGCAACAG ATAAATACAA GGACATCTAC 
ACTGAGCTGA GCATCGTGCG GGCCAAAGCG GAGCGGGATC TGGGCCGCCT GCGGGAGCAG 
CTGCAGCTGG CCCACAATGC ACTGGGGGAG CCGACGCTGG AGGACATGGA GCGCGGAGGA 
TACGATATCA TGAAGTCCAA GAGCAACCCG GATATATTAA AGATGGCGGC AGCAGCGGCA 
AAACGCTCCG AGAGGACAAT GAGGTCAAAG TCTGTGAGTC GTGATGTGCC CTGCGACAGC 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009304637.1
CDS	711..5993
Translation	MSSAKENTCRKFQANIFNKSKCQNCFKPRELHLLTDQDLNQAKPIYGGWLCLAPEGTDFDNPMQRSRKWQRRFFVLYEHGCLRFALDESPSTLPQGTVNMNQCTDVVDAEPRTGQKNALCIVTPEQEYFIRGDNKEIINGWSEQLAVYPRTNKQNQKKKRKVEPATSQEPGPAKVAVTGAGIPDAEKTPDSSSIIWQEELQSREADAAHTWSSEIPPVGSPLPSTGEGSTVSSVNGDEVDRCGLSFHSPVSQAPVDPLSPTGSSSSRQSAETGGVPRCLSPAPSDPFPSGSSLLSNGSHISGSMSSLDSEASGSTVTSSDSHPPEPRALKRSRLRSPQTRERELVLSPERSGRSNVIEKLEALELEQQERMEVQESSSTEARQGRSQNRRFQRQEQKTDAAQALDFHPPLRRAKSLDRRTTESVMTPDLLNFKKGWMVKLDEEGQWKKYWFVLTDHSLRYYKDSIAEEASDLDGEIDLSTCYNVTEYQAQRNYGFQIYTQEAVHTLSAMTAGIRRNWIQAVMKNVRPATAPDVASLTDEHGSFEGVCRPDVTQDSPSSEASVERETGAKKSRARERRREGRSKTFDWAEFRPIAQALAQQRAQEAELTERSKRREERRRRYESVTGATFDPALMEQEGGVAQTEAPPLSAEWQQRVEDEIEQRWQQVEKTPIRDERRVPLGSAHSRDATDMEKLLEGYKRGVEELKAQLENCHQQLLDSNRHKQDLEGKLKMAVEREQQTRAGYISPLESPLSVETEPQMKRTELVSSQAQSLTKKYQETKELLQLQELKKRDMQAQLGLSLSHWPAKDPSLSDTQKHEVCPSKCTRRTLTLTLQDSEGTIRELEHLINTDDPLTLREFIKLVQLHSEHVCVVESPPGMEQSEHSRSVGAECQKLLESLKNQQEMESEAVRRSLEKAGDCIRDYEARLLTMEDMLGRAQQQKAEILLQSPSRVEEFSADMSSRDLSQQVGILANENVALNQRYQEIVNQLREADREIDRLKAEVYRLRHSKEDDEESADKRELCEKSHKLQEALIKLETLGSNLKDTEKRLQLKEATLRGLGFQVAESEAEDDLKCQLEILQCKLSEKEKMCEELQSQNPRQEDAVRLNQMLVEAQEEIAGLKRKLESRAGFSSESEFMEEEGASELKRKSESLMLVFGALVNGREAECDAFIVDEDHAGLMTERRILCRIVDGLQKDEQSVRQVVERMMEDNNMLIHANKLSDIQPGETMHDEDDADIMKSLSEAVQSKVALLNQTSLARTGDKLTLRSRSDYIREAITDITHMYQTLRLKKEFARAEMRTQQELAVTRIHIEGEPIDSLDKSIQLQDLMARHKKELRELKEVYETDVEKLKKELEKAGETLKMRSEENVREIDSLTVCMENLEKKHEAERRELQTRFSLQMEELWEAVGFEDKDWSSGSLKEQILKLVRRESELTQLMHQKDGDLRLRYEKDLEKLKATCERGFAAMEESHQKVIEELQKKHQRELEKLQEEKERLLEEETAATIAAIEAMKNAHRSELERELEKARKATSSTENADIEEIHKQHEEELVSYQREIEVLSEQYSQKCLENAHLAQALEAERQALRQCQRENQELNAHNQELNNRLAAEITKMRSMATEDGADTGGLIQGKELYELEVMLRVKESEVQYLKQEINSLKDELQTAQRDKKYATDKYKDIYTELSIVRAKAERDLGRLREQLQLAHNALGEPTLEDMERGGYDIMKSKSNPDILKMAAAAAKRSERTMRSKSVSRDVPCDS

Target ORF information:

RefSeq Version	XM_009306362.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio myosin phosphatase Rho interacting protein (mprip), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009306362.2

ORF Insert Sequence:

ATGTCGAGCG CGAAGGAAAA CACGTGTAGG AAATTTCAGG CGAACATTTT CAACAAAAGC 
AAGTGTCAGA ACTGCTTCAA GCCGCGAGAG CTGCATCTGC TGACGGATCA GGATCTCAAT 
CAGGCCAAGC CCATCTATGG TGGATGGCTG TGTTTGGCTC CGGAGGGGAC GGATTTTGAC 
AACCCCATGC AGAGATCACG GAAATGGCAG CGGCGATTCT TCGTCCTGTA TGAGCACGGC 
TGCTTGCGCT TCGCTCTGGA TGAATCGCCC AGCACGCTGC CGCAGGGAAC GGTGAACATG 
AACCAGTGCA CAGACGTGGT GGACGCGGAG CCGCGCACGG GCCAGAAGAA CGCGCTGTGC 
ATCGTGACGC CCGAGCAGGA GTATTTCATC CGCGGAGACA ATAAAGAGAT CATCAACGGA 
TGGAGTGAGC AGCTAGCGGT GTATCCCAGA ACCAACAAGC AGAACCAGAA GAAGAAGCGC 
AAGGTGGAGC CGGCCACCTC ACAGGAGCCG GGTCCGGCTA AGGTGGCGGT GACGGGCGCC 
GGCATCCCTG ACGCAGAGAA AACTCCAGAC TCCAGCTCCA TCATCTGGCA GGAGGAGCTG 
CAGAGCAGAG AAGCAGACGC CGCTCACACC TGGAGCAGCG AGATTCCTCC TGTCGGCTCA 
CCGCTGCCCT CTACAGGCGA GGGGTCGACG GTGAGCTCAG TGAATGGGGA TGAGGTTGAC 
CGCTGCGGAC TTTCCTTCCA CTCGCCGGTC TCTCAGGCTC CTGTAGACCC GCTGTCCCCG 
ACAGGCAGCA GCTCCAGCAG GCAGAGTGCA GAGACGGGCG GGGTCCCTCG CTGCCTATCC 
CCGGCTCCCA GCGACCCCTT CCCCTCAGGC AGCAGCCTCC TGTCCAACGG CTCGCACATC 
AGCGGCTCTA TGAGCTCTCT GGACTCGGAG GCTAGCGGCA GCACCGTCAC GAGCAGCGAC 
AGCCACCCGC CGGAGCCCCG CGCCCTCAAA CGAAGCCGCC TACGGAGCCC ACAGACCCGG 
GAGCGGGAGC TAGTGCTGAG CCCTGAGAGA AGCGGCCGCT CGAATGTGAT TGAGAAGCTG 
GAGGCGCTGG AGCTGGAGCA GCAGGAGCGC ATGGAGGTTC AGGAGTCCAG CAGCACTGAG 
GCCCGACAGG GGCGGAGTCA GAACAGGAGA TTCCAGCGGC AGGAGCAGAA GACGGACGCA 
GCACAGGCTC TAGACTTCCA TCCTCCTCTA AGAAGAGCCA AATCTCTGGA CCGCAGGACC 
ACTGAGTCCG TCATGACCCC TGATCTGCTG AACTTTAAGA AAGGCTGGAT GGTGAAGCTG 
GATGAAGAAG GACAGTGGAA GAAGTATTGG TTTGTGCTGA CGGATCACAG TCTGCGATAC 
TACAAGGACT CCATCGCTGA AGAGGCGTCA GATCTGGATG GAGAGATCGA CCTCAGCACA 
TGCTATAATG TTACAGAGTA TCAGGCGCAG AGGAACTACG GCTTCCAGAT TTACACTCAG 
GAGGCGGTGC ACACACTCTC CGCCATGACT GCAGGAATCC GCCGTAACTG GATCCAGGCA 
GTGATGAAGA ATGTCCGGCC AGCCACTGCT CCAGATGTAG CCAGTCTGAC GGATGAGCAT 
GGGTCGTTTG AGGGTGTCTG TCGACCGGAC GTGACGCAGG ACTCTCCCTC CTCTGAGGCG 
TCTGTGGAGC GGGAAACAGG GGCCAAAAAA AGCCGTGCGC GGGAACGCAG GCGAGAGGGG 
CGGAGCAAAA CTTTCGACTG GGCGGAGTTT CGTCCCATCG CTCAGGCATT GGCTCAGCAG 
AGAGCGCAGG AGGCGGAGCT TACAGAGCGC AGCAAGAGGA GAGAGGAGCG AAGGCGGAGG 
TATGAGAGCG TGACCGGAGC AACCTTTGAT CCTGCACTGA TGGAGCAAGA GGGGGGCGTG 
GCTCAGACTG AAGCTCCGCC CCTGTCGGCT GAATGGCAGC AGCGAGTGGA GGATGAGATC 
GAGCAACGCT GGCAGCAGGT GGAGAAAACA CCAATCAGAG ACGAGCGCAG GGTTCCGTTA 
GGCTCCGCCC ACAGCAGAGA CGCCACAGAC ATGGAGAAGC TGCTGGAGGG ATACAAGAGA 
GGGGTGGAGG AGCTCAAGGC TCAGCTGGAG AACTGCCATC AGCAGCTTCT GGACTCCAAT 
CGGCACAAGC AGGATCTGGA GGGAAAACTG AAGATGGCGG TGGAGCGCGA GCAGCAGACG 
CGAGCCGGGT ACATCTCTCC GCTGGAGTCC CCTTTGAGTG TTGAGACAGA GCCGCAGATG 
AAGAGGACTG AACTGGTTAG TTCTCAAGCT CAGAGTTTAA CAAAGAAGTA CCAGGAAACC 
AAAGAGCTCC TGCAGCTGCA AGAACTGAAA AAGCGCGACA TGCAGGCACA GCTTGGCCTT 
TCTCTCTCAC ACTGGCCCGC AAAGGACCCT TCGCTTTCTG ACACCCAAAA ACACGAGGTC 
TGTCCCTCGA AGTGCACTCG GCGAACGCTC ACGCTTACTC TTCAGGATAG CGAGGGAACG 
ATTCGAGAGC TTGAGCACTT AATAAACACT GACGATCCTT TAACGTTGAG GGAGTTTATA 
AAGCTCGTCC AGTTGCATAG TGAACATGTT TGCGTGGTAG AATCTCCTCC AGGGATGGAG 
CAAAGTGAAC ACAGCCGCTC AGTTGGAGCG GAGTGCCAGA AACTGCTGGA AAGCTTGAAG 
AACCAGCAGG AGATGGAGAG CGAAGCTGTC AGGAGAAGTT TGGAAAAGGC TGGAGATTGC 
ATTCGAGACT ATGAAGCGCG TTTGCTCACT ATGGAGGACA TGCTAGGACG AGCTCAGCAG 
CAAAAAGCGG AGATTCTCCT GCAGTCCCCA TCGAGAGTAG AAGAGTTCAG CGCTGATATG 
AGCAGCAGAG ATCTGTCGCA GCAGGTGGGG ATTCTGGCCA ATGAAAATGT AGCGTTGAAT 
CAACGCTATC AGGAGATCGT GAACCAACTG AGAGAAGCAG ATCGAGAGAT AGACAGGCTG 
AAGGCGGAGG TGTATAGACT GCGACACAGT AAGGAAGACG ATGAAGAGTC CGCAGATAAG 
AGAGAGCTCT GCGAAAAATC CCACAAGCTT CAAGAAGCTC TGATTAAATT AGAGACGCTT 
GGTAGTAATC TGAAAGACAC TGAGAAAAGA TTGCAGCTAA AAGAAGCCAC TCTTCGTGGA 
CTTGGATTTC AGGTGGCGGA AAGTGAAGCT GAAGACGATC TCAAGTGTCA GTTGGAGATC 
CTTCAATGCA AGCTTTCTGA GAAAGAGAAG ATGTGCGAAG AACTCCAGTC GCAGAATCCA 
AGACAAGAGG ACGCGGTGAG GCTCAATCAG ATGCTTGTAG AAGCTCAGGA GGAGATCGCA 
GGGTTGAAAC GGAAGCTAGA AAGTCGAGCA GGGTTCAGTA GCGAAAGTGA GTTTATGGAA 
GAGGAAGGTG CGAGTGAGTT AAAGAGAAAG TCAGAGTCTC TAATGCTTGT GTTTGGTGCG 
TTAGTAAACG GCAGGGAGGC AGAATGCGAT GCGTTTATTG TGGATGAGGA TCACGCAGGA 
TTGATGACGG AGAGAAGGAT CTTATGCAGG ATTGTAGACG GTCTGCAGAA AGACGAGCAA 
TCTGTGAGGC AAGTGGTAGA GCGAATGATG GAGGATAATA ACATGCTGAT TCATGCAAAC 
AAGCTCTCCG ACATTCAACC AGGAGAGACG ATGCATGATG AAGATGACGC AGACATCATG 
AAGAGTCTCT CTGAAGCGGT TCAGAGCAAA GTGGCGTTAT TAAATCAGAC TTCTTTAGCG 
AGAACGGGTG ACAAACTCAC TTTGAGAAGC AGATCTGACT ACATACGTGA AGCAATCACA 
GACATAACAC ACATGTACCA AACGCTCAGG CTGAAGAAAG AGTTTGCACG TGCTGAAATG 
AGGACGCAAC AGGAATTAGC AGTGACGCGC ATTCATATCG AAGGAGAGCC GATCGACTCT 
TTGGATAAAA GCATTCAGCT GCAGGATCTG ATGGCGAGAC ATAAGAAGGA GCTTCGAGAG 
CTGAAGGAGG TTTACGAGAC GGATGTGGAA AAGCTTAAAA AGGAGTTGGA GAAAGCCGGA 
GAAACCCTGA AGATGAGATC TGAAGAGAAC GTCCGAGAGA TCGACTCGCT GACCGTGTGC 
ATGGAGAACC TGGAGAAAAA GCACGAAGCC GAAAGGCGAG AATTACAGAC ACGCTTCAGC 
CTGCAAATGG AGGAGCTCTG GGAAGCTGTC GGGTTTGAGG ATAAAGATTG GAGTTCGGGA 
TCCCTCAAAG AGCAAATCCT GAAGCTGGTG CGCCGAGAGT CTGAACTGAC CCAGCTAATG 
CATCAGAAAG ATGGAGACCT GCGTCTGAGA TACGAGAAAG ATCTGGAAAA GCTAAAGGCC 
ACATGTGAGC GAGGTTTCGC CGCTATGGAG GAGTCGCACC AGAAGGTGAT CGAGGAGCTG 
CAGAAGAAAC ACCAGAGAGA GCTGGAGAAG CTGCAGGAGG AGAAGGAGAG ACTGCTGGAG 
GAGGAGACGG CTGCTACCAT CGCCGCTATC GAGGCGATGA AGAACGCACA CCGGTCAGAG 
CTGGAGCGAG AGCTGGAGAA AGCACGCAAA GCCACCAGCA GCACAGAGAA CGCAGACATC 
GAGGAGATCC ACAAACAGCA CGAGGAGGAG CTGGTGTCGT ATCAGCGGGA GATCGAGGTG 
CTGTCAGAGC AGTATTCACA GAAGTGTCTG GAGAACGCTC ATCTGGCTCA GGCGCTGGAG 
GCCGAGCGGC AGGCGCTCAG ACAGTGCCAG AGGGAGAACC AGGAGCTCAA TGCACACAAC 
CAGGAGCTCA ACAATCGTCT GGCTGCAGAG ATCACCAAGA TGCGCTCGAT GGCGACTGAA 
GACGGAGCTG ACACTGGAGG ACTGATTCAA GGGAAGGAGC TGTACGAGCT GGAGGTGATG 
CTGAGGGTGA AGGAGTCAGA AGTGCAGTAT CTGAAGCAGG AGATCAACTC TCTGAAAGAT 
GAGCTCCAGA CGGCACAAAG AGATAAGAAA TATGCAACAG ATAAATACAA GGACATCTAC 
ACTGAGCTGA GCATCGTGCG GGCCAAAGCG GAGCGGGATC TGGGCCGCCT GCGGGAGCAG 
CTGCAGCTGG CCCACAATGC ACTGGGGGAG CCGACGCTGG AGGACATGGA GCGCGGAGGA 
TACGATATCA TGAAGTCCAA GAGCAACCCG GATATATTAA AGATGGCGGC AGCAGCGGCA 
AAACGCTCCG AGAGGACAAT GAGGTCAAAG TCTGTGAGTC GTGATGTGCC CTGCGACAGC 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

mprip ( XM_021480198.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021335873.1 Danio rerio myosin phosphatase Rho interacting protein (mprip), transcript variant X3, mRNA.

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CloneID	ODa42922
Clone ID Related Accession (Same CDS sequence)	XM_009306362.2 , XM_021480198.1 , XM_021480197.1
Accession Version	XM_021480198.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5283bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	myosin phosphatase Rho-interacting protein isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007123.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGAGCG CGAAGGAAAA CACGTGTAGG AAATTTCAGG CGAACATTTT CAACAAAAGC 
AAGTGTCAGA ACTGCTTCAA GCCGCGAGAG CTGCATCTGC TGACGGATCA GGATCTCAAT 
CAGGCCAAGC CCATCTATGG TGGATGGCTG TGTTTGGCTC CGGAGGGGAC GGATTTTGAC 
AACCCCATGC AGAGATCACG GAAATGGCAG CGGCGATTCT TCGTCCTGTA TGAGCACGGC 
TGCTTGCGCT TCGCTCTGGA TGAATCGCCC AGCACGCTGC CGCAGGGAAC GGTGAACATG 
AACCAGTGCA CAGACGTGGT GGACGCGGAG CCGCGCACGG GCCAGAAGAA CGCGCTGTGC 
ATCGTGACGC CCGAGCAGGA GTATTTCATC CGCGGAGACA ATAAAGAGAT CATCAACGGA 
TGGAGTGAGC AGCTAGCGGT GTATCCCAGA ACCAACAAGC AGAACCAGAA GAAGAAGCGC 
AAGGTGGAGC CGGCCACCTC ACAGGAGCCG GGTCCGGCTA AGGTGGCGGT GACGGGCGCC 
GGCATCCCTG ACGCAGAGAA AACTCCAGAC TCCAGCTCCA TCATCTGGCA GGAGGAGCTG 
CAGAGCAGAG AAGCAGACGC CGCTCACACC TGGAGCAGCG AGATTCCTCC TGTCGGCTCA 
CCGCTGCCCT CTACAGGCGA GGGGTCGACG GTGAGCTCAG TGAATGGGGA TGAGGTTGAC 
CGCTGCGGAC TTTCCTTCCA CTCGCCGGTC TCTCAGGCTC CTGTAGACCC GCTGTCCCCG 
ACAGGCAGCA GCTCCAGCAG GCAGAGTGCA GAGACGGGCG GGGTCCCTCG CTGCCTATCC 
CCGGCTCCCA GCGACCCCTT CCCCTCAGGC AGCAGCCTCC TGTCCAACGG CTCGCACATC 
AGCGGCTCTA TGAGCTCTCT GGACTCGGAG GCTAGCGGCA GCACCGTCAC GAGCAGCGAC 
AGCCACCCGC CGGAGCCCCG CGCCCTCAAA CGAAGCCGCC TACGGAGCCC ACAGACCCGG 
GAGCGGGAGC TAGTGCTGAG CCCTGAGAGA AGCGGCCGCT CGAATGTGAT TGAGAAGCTG 
GAGGCGCTGG AGCTGGAGCA GCAGGAGCGC ATGGAGGTTC AGGAGTCCAG CAGCACTGAG 
GCCCGACAGG GGCGGAGTCA GAACAGGAGA TTCCAGCGGC AGGAGCAGAA GACGGACGCA 
GCACAGGCTC TAGACTTCCA TCCTCCTCTA AGAAGAGCCA AATCTCTGGA CCGCAGGACC 
ACTGAGTCCG TCATGACCCC TGATCTGCTG AACTTTAAGA AAGGCTGGAT GGTGAAGCTG 
GATGAAGAAG GACAGTGGAA GAAGTATTGG TTTGTGCTGA CGGATCACAG TCTGCGATAC 
TACAAGGACT CCATCGCTGA AGAGGCGTCA GATCTGGATG GAGAGATCGA CCTCAGCACA 
TGCTATAATG TTACAGAGTA TCAGGCGCAG AGGAACTACG GCTTCCAGAT TTACACTCAG 
GAGGCGGTGC ACACACTCTC CGCCATGACT GCAGGAATCC GCCGTAACTG GATCCAGGCA 
GTGATGAAGA ATGTCCGGCC AGCCACTGCT CCAGATGTAG CCAGTCTGAC GGATGAGCAT 
GGGTCGTTTG AGGGTGTCTG TCGACCGGAC GTGACGCAGG ACTCTCCCTC CTCTGAGGCG 
TCTGTGGAGC GGGAAACAGG GGCCAAAAAA AGCCGTGCGC GGGAACGCAG GCGAGAGGGG 
CGGAGCAAAA CTTTCGACTG GGCGGAGTTT CGTCCCATCG CTCAGGCATT GGCTCAGCAG 
AGAGCGCAGG AGGCGGAGCT TACAGAGCGC AGCAAGAGGA GAGAGGAGCG AAGGCGGAGG 
TATGAGAGCG TGACCGGAGC AACCTTTGAT CCTGCACTGA TGGAGCAAGA GGGGGGCGTG 
GCTCAGACTG AAGCTCCGCC CCTGTCGGCT GAATGGCAGC AGCGAGTGGA GGATGAGATC 
GAGCAACGCT GGCAGCAGGT GGAGAAAACA CCAATCAGAG ACGAGCGCAG GGTTCCGTTA 
GGCTCCGCCC ACAGCAGAGA CGCCACAGAC ATGGAGAAGC TGCTGGAGGG ATACAAGAGA 
GGGGTGGAGG AGCTCAAGGC TCAGCTGGAG AACTGCCATC AGCAGCTTCT GGACTCCAAT 
CGGCACAAGC AGGATCTGGA GGGAAAACTG AAGATGGCGG TGGAGCGCGA GCAGCAGACG 
CGAGCCGGGT ACATCTCTCC GCTGGAGTCC CCTTTGAGTG TTGAGACAGA GCCGCAGATG 
AAGAGGACTG AACTGGTTAG TTCTCAAGCT CAGAGTTTAA CAAAGAAGTA CCAGGAAACC 
AAAGAGCTCC TGCAGCTGCA AGAACTGAAA AAGCGCGACA TGCAGGCACA GCTTGGCCTT 
TCTCTCTCAC ACTGGCCCGC AAAGGACCCT TCGCTTTCTG ACACCCAAAA ACACGAGGTC 
TGTCCCTCGA AGTGCACTCG GCGAACGCTC ACGCTTACTC TTCAGGATAG CGAGGGAACG 
ATTCGAGAGC TTGAGCACTT AATAAACACT GACGATCCTT TAACGTTGAG GGAGTTTATA 
AAGCTCGTCC AGTTGCATAG TGAACATGTT TGCGTGGTAG AATCTCCTCC AGGGATGGAG 
CAAAGTGAAC ACAGCCGCTC AGTTGGAGCG GAGTGCCAGA AACTGCTGGA AAGCTTGAAG 
AACCAGCAGG AGATGGAGAG CGAAGCTGTC AGGAGAAGTT TGGAAAAGGC TGGAGATTGC 
ATTCGAGACT ATGAAGCGCG TTTGCTCACT ATGGAGGACA TGCTAGGACG AGCTCAGCAG 
CAAAAAGCGG AGATTCTCCT GCAGTCCCCA TCGAGAGTAG AAGAGTTCAG CGCTGATATG 
AGCAGCAGAG ATCTGTCGCA GCAGGTGGGG ATTCTGGCCA ATGAAAATGT AGCGTTGAAT 
CAACGCTATC AGGAGATCGT GAACCAACTG AGAGAAGCAG ATCGAGAGAT AGACAGGCTG 
AAGGCGGAGG TGTATAGACT GCGACACAGT AAGGAAGACG ATGAAGAGTC CGCAGATAAG 
AGAGAGCTCT GCGAAAAATC CCACAAGCTT CAAGAAGCTC TGATTAAATT AGAGACGCTT 
GGTAGTAATC TGAAAGACAC TGAGAAAAGA TTGCAGCTAA AAGAAGCCAC TCTTCGTGGA 
CTTGGATTTC AGGTGGCGGA AAGTGAAGCT GAAGACGATC TCAAGTGTCA GTTGGAGATC 
CTTCAATGCA AGCTTTCTGA GAAAGAGAAG ATGTGCGAAG AACTCCAGTC GCAGAATCCA 
AGACAAGAGG ACGCGGTGAG GCTCAATCAG ATGCTTGTAG AAGCTCAGGA GGAGATCGCA 
GGGTTGAAAC GGAAGCTAGA AAGTCGAGCA GGGTTCAGTA GCGAAAGTGA GTTTATGGAA 
GAGGAAGGTG CGAGTGAGTT AAAGAGAAAG TCAGAGTCTC TAATGCTTGT GTTTGGTGCG 
TTAGTAAACG GCAGGGAGGC AGAATGCGAT GCGTTTATTG TGGATGAGGA TCACGCAGGA 
TTGATGACGG AGAGAAGGAT CTTATGCAGG ATTGTAGACG GTCTGCAGAA AGACGAGCAA 
TCTGTGAGGC AAGTGGTAGA GCGAATGATG GAGGATAATA ACATGCTGAT TCATGCAAAC 
AAGCTCTCCG ACATTCAACC AGGAGAGACG ATGCATGATG AAGATGACGC AGACATCATG 
AAGAGTCTCT CTGAAGCGGT TCAGAGCAAA GTGGCGTTAT TAAATCAGAC TTCTTTAGCG 
AGAACGGGTG ACAAACTCAC TTTGAGAAGC AGATCTGACT ACATACGTGA AGCAATCACA 
GACATAACAC ACATGTACCA AACGCTCAGG CTGAAGAAAG AGTTTGCACG TGCTGAAATG 
AGGACGCAAC AGGAATTAGC AGTGACGCGC ATTCATATCG AAGGAGAGCC GATCGACTCT 
TTGGATAAAA GCATTCAGCT GCAGGATCTG ATGGCGAGAC ATAAGAAGGA GCTTCGAGAG 
CTGAAGGAGG TTTACGAGAC GGATGTGGAA AAGCTTAAAA AGGAGTTGGA GAAAGCCGGA 
GAAACCCTGA AGATGAGATC TGAAGAGAAC GTCCGAGAGA TCGACTCGCT GACCGTGTGC 
ATGGAGAACC TGGAGAAAAA GCACGAAGCC GAAAGGCGAG AATTACAGAC ACGCTTCAGC 
CTGCAAATGG AGGAGCTCTG GGAAGCTGTC GGGTTTGAGG ATAAAGATTG GAGTTCGGGA 
TCCCTCAAAG AGCAAATCCT GAAGCTGGTG CGCCGAGAGT CTGAACTGAC CCAGCTAATG 
CATCAGAAAG ATGGAGACCT GCGTCTGAGA TACGAGAAAG ATCTGGAAAA GCTAAAGGCC 
ACATGTGAGC GAGGTTTCGC CGCTATGGAG GAGTCGCACC AGAAGGTGAT CGAGGAGCTG 
CAGAAGAAAC ACCAGAGAGA GCTGGAGAAG CTGCAGGAGG AGAAGGAGAG ACTGCTGGAG 
GAGGAGACGG CTGCTACCAT CGCCGCTATC GAGGCGATGA AGAACGCACA CCGGTCAGAG 
CTGGAGCGAG AGCTGGAGAA AGCACGCAAA GCCACCAGCA GCACAGAGAA CGCAGACATC 
GAGGAGATCC ACAAACAGCA CGAGGAGGAG CTGGTGTCGT ATCAGCGGGA GATCGAGGTG 
CTGTCAGAGC AGTATTCACA GAAGTGTCTG GAGAACGCTC ATCTGGCTCA GGCGCTGGAG 
GCCGAGCGGC AGGCGCTCAG ACAGTGCCAG AGGGAGAACC AGGAGCTCAA TGCACACAAC 
CAGGAGCTCA ACAATCGTCT GGCTGCAGAG ATCACCAAGA TGCGCTCGAT GGCGACTGAA 
GACGGAGCTG ACACTGGAGG ACTGATTCAA GGGAAGGAGC TGTACGAGCT GGAGGTGATG 
CTGAGGGTGA AGGAGTCAGA AGTGCAGTAT CTGAAGCAGG AGATCAACTC TCTGAAAGAT 
GAGCTCCAGA CGGCACAAAG AGATAAGAAA TATGCAACAG ATAAATACAA GGACATCTAC 
ACTGAGCTGA GCATCGTGCG GGCCAAAGCG GAGCGGGATC TGGGCCGCCT GCGGGAGCAG 
CTGCAGCTGG CCCACAATGC ACTGGGGGAG CCGACGCTGG AGGACATGGA GCGCGGAGGA 
TACGATATCA TGAAGTCCAA GAGCAACCCG GATATATTAA AGATGGCGGC AGCAGCGGCA 
AAACGCTCCG AGAGGACAAT GAGGTCAAAG TCTGTGAGTC GTGATGTGCC CTGCGACAGC 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021335873.1
CDS	680..5962
Translation	MSSAKENTCRKFQANIFNKSKCQNCFKPRELHLLTDQDLNQAKPIYGGWLCLAPEGTDFDNPMQRSRKWQRRFFVLYEHGCLRFALDESPSTLPQGTVNMNQCTDVVDAEPRTGQKNALCIVTPEQEYFIRGDNKEIINGWSEQLAVYPRTNKQNQKKKRKVEPATSQEPGPAKVAVTGAGIPDAEKTPDSSSIIWQEELQSREADAAHTWSSEIPPVGSPLPSTGEGSTVSSVNGDEVDRCGLSFHSPVSQAPVDPLSPTGSSSSRQSAETGGVPRCLSPAPSDPFPSGSSLLSNGSHISGSMSSLDSEASGSTVTSSDSHPPEPRALKRSRLRSPQTRERELVLSPERSGRSNVIEKLEALELEQQERMEVQESSSTEARQGRSQNRRFQRQEQKTDAAQALDFHPPLRRAKSLDRRTTESVMTPDLLNFKKGWMVKLDEEGQWKKYWFVLTDHSLRYYKDSIAEEASDLDGEIDLSTCYNVTEYQAQRNYGFQIYTQEAVHTLSAMTAGIRRNWIQAVMKNVRPATAPDVASLTDEHGSFEGVCRPDVTQDSPSSEASVERETGAKKSRARERRREGRSKTFDWAEFRPIAQALAQQRAQEAELTERSKRREERRRRYESVTGATFDPALMEQEGGVAQTEAPPLSAEWQQRVEDEIEQRWQQVEKTPIRDERRVPLGSAHSRDATDMEKLLEGYKRGVEELKAQLENCHQQLLDSNRHKQDLEGKLKMAVEREQQTRAGYISPLESPLSVETEPQMKRTELVSSQAQSLTKKYQETKELLQLQELKKRDMQAQLGLSLSHWPAKDPSLSDTQKHEVCPSKCTRRTLTLTLQDSEGTIRELEHLINTDDPLTLREFIKLVQLHSEHVCVVESPPGMEQSEHSRSVGAECQKLLESLKNQQEMESEAVRRSLEKAGDCIRDYEARLLTMEDMLGRAQQQKAEILLQSPSRVEEFSADMSSRDLSQQVGILANENVALNQRYQEIVNQLREADREIDRLKAEVYRLRHSKEDDEESADKRELCEKSHKLQEALIKLETLGSNLKDTEKRLQLKEATLRGLGFQVAESEAEDDLKCQLEILQCKLSEKEKMCEELQSQNPRQEDAVRLNQMLVEAQEEIAGLKRKLESRAGFSSESEFMEEEGASELKRKSESLMLVFGALVNGREAECDAFIVDEDHAGLMTERRILCRIVDGLQKDEQSVRQVVERMMEDNNMLIHANKLSDIQPGETMHDEDDADIMKSLSEAVQSKVALLNQTSLARTGDKLTLRSRSDYIREAITDITHMYQTLRLKKEFARAEMRTQQELAVTRIHIEGEPIDSLDKSIQLQDLMARHKKELRELKEVYETDVEKLKKELEKAGETLKMRSEENVREIDSLTVCMENLEKKHEAERRELQTRFSLQMEELWEAVGFEDKDWSSGSLKEQILKLVRRESELTQLMHQKDGDLRLRYEKDLEKLKATCERGFAAMEESHQKVIEELQKKHQRELEKLQEEKERLLEEETAATIAAIEAMKNAHRSELERELEKARKATSSTENADIEEIHKQHEEELVSYQREIEVLSEQYSQKCLENAHLAQALEAERQALRQCQRENQELNAHNQELNNRLAAEITKMRSMATEDGADTGGLIQGKELYELEVMLRVKESEVQYLKQEINSLKDELQTAQRDKKYATDKYKDIYTELSIVRAKAERDLGRLREQLQLAHNALGEPTLEDMERGGYDIMKSKSNPDILKMAAAAAKRSERTMRSKSVSRDVPCDS

Target ORF information:

RefSeq Version	XM_021480198.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio myosin phosphatase Rho interacting protein (mprip), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021480198.1

ORF Insert Sequence:

ATGTCGAGCG CGAAGGAAAA CACGTGTAGG AAATTTCAGG CGAACATTTT CAACAAAAGC 
AAGTGTCAGA ACTGCTTCAA GCCGCGAGAG CTGCATCTGC TGACGGATCA GGATCTCAAT 
CAGGCCAAGC CCATCTATGG TGGATGGCTG TGTTTGGCTC CGGAGGGGAC GGATTTTGAC 
AACCCCATGC AGAGATCACG GAAATGGCAG CGGCGATTCT TCGTCCTGTA TGAGCACGGC 
TGCTTGCGCT TCGCTCTGGA TGAATCGCCC AGCACGCTGC CGCAGGGAAC GGTGAACATG 
AACCAGTGCA CAGACGTGGT GGACGCGGAG CCGCGCACGG GCCAGAAGAA CGCGCTGTGC 
ATCGTGACGC CCGAGCAGGA GTATTTCATC CGCGGAGACA ATAAAGAGAT CATCAACGGA 
TGGAGTGAGC AGCTAGCGGT GTATCCCAGA ACCAACAAGC AGAACCAGAA GAAGAAGCGC 
AAGGTGGAGC CGGCCACCTC ACAGGAGCCG GGTCCGGCTA AGGTGGCGGT GACGGGCGCC 
GGCATCCCTG ACGCAGAGAA AACTCCAGAC TCCAGCTCCA TCATCTGGCA GGAGGAGCTG 
CAGAGCAGAG AAGCAGACGC CGCTCACACC TGGAGCAGCG AGATTCCTCC TGTCGGCTCA 
CCGCTGCCCT CTACAGGCGA GGGGTCGACG GTGAGCTCAG TGAATGGGGA TGAGGTTGAC 
CGCTGCGGAC TTTCCTTCCA CTCGCCGGTC TCTCAGGCTC CTGTAGACCC GCTGTCCCCG 
ACAGGCAGCA GCTCCAGCAG GCAGAGTGCA GAGACGGGCG GGGTCCCTCG CTGCCTATCC 
CCGGCTCCCA GCGACCCCTT CCCCTCAGGC AGCAGCCTCC TGTCCAACGG CTCGCACATC 
AGCGGCTCTA TGAGCTCTCT GGACTCGGAG GCTAGCGGCA GCACCGTCAC GAGCAGCGAC 
AGCCACCCGC CGGAGCCCCG CGCCCTCAAA CGAAGCCGCC TACGGAGCCC ACAGACCCGG 
GAGCGGGAGC TAGTGCTGAG CCCTGAGAGA AGCGGCCGCT CGAATGTGAT TGAGAAGCTG 
GAGGCGCTGG AGCTGGAGCA GCAGGAGCGC ATGGAGGTTC AGGAGTCCAG CAGCACTGAG 
GCCCGACAGG GGCGGAGTCA GAACAGGAGA TTCCAGCGGC AGGAGCAGAA GACGGACGCA 
GCACAGGCTC TAGACTTCCA TCCTCCTCTA AGAAGAGCCA AATCTCTGGA CCGCAGGACC 
ACTGAGTCCG TCATGACCCC TGATCTGCTG AACTTTAAGA AAGGCTGGAT GGTGAAGCTG 
GATGAAGAAG GACAGTGGAA GAAGTATTGG TTTGTGCTGA CGGATCACAG TCTGCGATAC 
TACAAGGACT CCATCGCTGA AGAGGCGTCA GATCTGGATG GAGAGATCGA CCTCAGCACA 
TGCTATAATG TTACAGAGTA TCAGGCGCAG AGGAACTACG GCTTCCAGAT TTACACTCAG 
GAGGCGGTGC ACACACTCTC CGCCATGACT GCAGGAATCC GCCGTAACTG GATCCAGGCA 
GTGATGAAGA ATGTCCGGCC AGCCACTGCT CCAGATGTAG CCAGTCTGAC GGATGAGCAT 
GGGTCGTTTG AGGGTGTCTG TCGACCGGAC GTGACGCAGG ACTCTCCCTC CTCTGAGGCG 
TCTGTGGAGC GGGAAACAGG GGCCAAAAAA AGCCGTGCGC GGGAACGCAG GCGAGAGGGG 
CGGAGCAAAA CTTTCGACTG GGCGGAGTTT CGTCCCATCG CTCAGGCATT GGCTCAGCAG 
AGAGCGCAGG AGGCGGAGCT TACAGAGCGC AGCAAGAGGA GAGAGGAGCG AAGGCGGAGG 
TATGAGAGCG TGACCGGAGC AACCTTTGAT CCTGCACTGA TGGAGCAAGA GGGGGGCGTG 
GCTCAGACTG AAGCTCCGCC CCTGTCGGCT GAATGGCAGC AGCGAGTGGA GGATGAGATC 
GAGCAACGCT GGCAGCAGGT GGAGAAAACA CCAATCAGAG ACGAGCGCAG GGTTCCGTTA 
GGCTCCGCCC ACAGCAGAGA CGCCACAGAC ATGGAGAAGC TGCTGGAGGG ATACAAGAGA 
GGGGTGGAGG AGCTCAAGGC TCAGCTGGAG AACTGCCATC AGCAGCTTCT GGACTCCAAT 
CGGCACAAGC AGGATCTGGA GGGAAAACTG AAGATGGCGG TGGAGCGCGA GCAGCAGACG 
CGAGCCGGGT ACATCTCTCC GCTGGAGTCC CCTTTGAGTG TTGAGACAGA GCCGCAGATG 
AAGAGGACTG AACTGGTTAG TTCTCAAGCT CAGAGTTTAA CAAAGAAGTA CCAGGAAACC 
AAAGAGCTCC TGCAGCTGCA AGAACTGAAA AAGCGCGACA TGCAGGCACA GCTTGGCCTT 
TCTCTCTCAC ACTGGCCCGC AAAGGACCCT TCGCTTTCTG ACACCCAAAA ACACGAGGTC 
TGTCCCTCGA AGTGCACTCG GCGAACGCTC ACGCTTACTC TTCAGGATAG CGAGGGAACG 
ATTCGAGAGC TTGAGCACTT AATAAACACT GACGATCCTT TAACGTTGAG GGAGTTTATA 
AAGCTCGTCC AGTTGCATAG TGAACATGTT TGCGTGGTAG AATCTCCTCC AGGGATGGAG 
CAAAGTGAAC ACAGCCGCTC AGTTGGAGCG GAGTGCCAGA AACTGCTGGA AAGCTTGAAG 
AACCAGCAGG AGATGGAGAG CGAAGCTGTC AGGAGAAGTT TGGAAAAGGC TGGAGATTGC 
ATTCGAGACT ATGAAGCGCG TTTGCTCACT ATGGAGGACA TGCTAGGACG AGCTCAGCAG 
CAAAAAGCGG AGATTCTCCT GCAGTCCCCA TCGAGAGTAG AAGAGTTCAG CGCTGATATG 
AGCAGCAGAG ATCTGTCGCA GCAGGTGGGG ATTCTGGCCA ATGAAAATGT AGCGTTGAAT 
CAACGCTATC AGGAGATCGT GAACCAACTG AGAGAAGCAG ATCGAGAGAT AGACAGGCTG 
AAGGCGGAGG TGTATAGACT GCGACACAGT AAGGAAGACG ATGAAGAGTC CGCAGATAAG 
AGAGAGCTCT GCGAAAAATC CCACAAGCTT CAAGAAGCTC TGATTAAATT AGAGACGCTT 
GGTAGTAATC TGAAAGACAC TGAGAAAAGA TTGCAGCTAA AAGAAGCCAC TCTTCGTGGA 
CTTGGATTTC AGGTGGCGGA AAGTGAAGCT GAAGACGATC TCAAGTGTCA GTTGGAGATC 
CTTCAATGCA AGCTTTCTGA GAAAGAGAAG ATGTGCGAAG AACTCCAGTC GCAGAATCCA 
AGACAAGAGG ACGCGGTGAG GCTCAATCAG ATGCTTGTAG AAGCTCAGGA GGAGATCGCA 
GGGTTGAAAC GGAAGCTAGA AAGTCGAGCA GGGTTCAGTA GCGAAAGTGA GTTTATGGAA 
GAGGAAGGTG CGAGTGAGTT AAAGAGAAAG TCAGAGTCTC TAATGCTTGT GTTTGGTGCG 
TTAGTAAACG GCAGGGAGGC AGAATGCGAT GCGTTTATTG TGGATGAGGA TCACGCAGGA 
TTGATGACGG AGAGAAGGAT CTTATGCAGG ATTGTAGACG GTCTGCAGAA AGACGAGCAA 
TCTGTGAGGC AAGTGGTAGA GCGAATGATG GAGGATAATA ACATGCTGAT TCATGCAAAC 
AAGCTCTCCG ACATTCAACC AGGAGAGACG ATGCATGATG AAGATGACGC AGACATCATG 
AAGAGTCTCT CTGAAGCGGT TCAGAGCAAA GTGGCGTTAT TAAATCAGAC TTCTTTAGCG 
AGAACGGGTG ACAAACTCAC TTTGAGAAGC AGATCTGACT ACATACGTGA AGCAATCACA 
GACATAACAC ACATGTACCA AACGCTCAGG CTGAAGAAAG AGTTTGCACG TGCTGAAATG 
AGGACGCAAC AGGAATTAGC AGTGACGCGC ATTCATATCG AAGGAGAGCC GATCGACTCT 
TTGGATAAAA GCATTCAGCT GCAGGATCTG ATGGCGAGAC ATAAGAAGGA GCTTCGAGAG 
CTGAAGGAGG TTTACGAGAC GGATGTGGAA AAGCTTAAAA AGGAGTTGGA GAAAGCCGGA 
GAAACCCTGA AGATGAGATC TGAAGAGAAC GTCCGAGAGA TCGACTCGCT GACCGTGTGC 
ATGGAGAACC TGGAGAAAAA GCACGAAGCC GAAAGGCGAG AATTACAGAC ACGCTTCAGC 
CTGCAAATGG AGGAGCTCTG GGAAGCTGTC GGGTTTGAGG ATAAAGATTG GAGTTCGGGA 
TCCCTCAAAG AGCAAATCCT GAAGCTGGTG CGCCGAGAGT CTGAACTGAC CCAGCTAATG 
CATCAGAAAG ATGGAGACCT GCGTCTGAGA TACGAGAAAG ATCTGGAAAA GCTAAAGGCC 
ACATGTGAGC GAGGTTTCGC CGCTATGGAG GAGTCGCACC AGAAGGTGAT CGAGGAGCTG 
CAGAAGAAAC ACCAGAGAGA GCTGGAGAAG CTGCAGGAGG AGAAGGAGAG ACTGCTGGAG 
GAGGAGACGG CTGCTACCAT CGCCGCTATC GAGGCGATGA AGAACGCACA CCGGTCAGAG 
CTGGAGCGAG AGCTGGAGAA AGCACGCAAA GCCACCAGCA GCACAGAGAA CGCAGACATC 
GAGGAGATCC ACAAACAGCA CGAGGAGGAG CTGGTGTCGT ATCAGCGGGA GATCGAGGTG 
CTGTCAGAGC AGTATTCACA GAAGTGTCTG GAGAACGCTC ATCTGGCTCA GGCGCTGGAG 
GCCGAGCGGC AGGCGCTCAG ACAGTGCCAG AGGGAGAACC AGGAGCTCAA TGCACACAAC 
CAGGAGCTCA ACAATCGTCT GGCTGCAGAG ATCACCAAGA TGCGCTCGAT GGCGACTGAA 
GACGGAGCTG ACACTGGAGG ACTGATTCAA GGGAAGGAGC TGTACGAGCT GGAGGTGATG 
CTGAGGGTGA AGGAGTCAGA AGTGCAGTAT CTGAAGCAGG AGATCAACTC TCTGAAAGAT 
GAGCTCCAGA CGGCACAAAG AGATAAGAAA TATGCAACAG ATAAATACAA GGACATCTAC 
ACTGAGCTGA GCATCGTGCG GGCCAAAGCG GAGCGGGATC TGGGCCGCCT GCGGGAGCAG 
CTGCAGCTGG CCCACAATGC ACTGGGGGAG CCGACGCTGG AGGACATGGA GCGCGGAGGA 
TACGATATCA TGAAGTCCAA GAGCAACCCG GATATATTAA AGATGGCGGC AGCAGCGGCA 
AAACGCTCCG AGAGGACAAT GAGGTCAAAG TCTGTGAGTC GTGATGTGCC CTGCGACAGC 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

mprip ( XM_021480197.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021335872.1 Danio rerio myosin phosphatase Rho interacting protein (mprip), transcript variant X2, mRNA.

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CloneID	ODa42922
Clone ID Related Accession (Same CDS sequence)	XM_009306362.2 , XM_021480198.1 , XM_021480197.1
Accession Version	XM_021480197.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5283bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	myosin phosphatase Rho-interacting protein isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007123.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGAGCG CGAAGGAAAA CACGTGTAGG AAATTTCAGG CGAACATTTT CAACAAAAGC 
AAGTGTCAGA ACTGCTTCAA GCCGCGAGAG CTGCATCTGC TGACGGATCA GGATCTCAAT 
CAGGCCAAGC CCATCTATGG TGGATGGCTG TGTTTGGCTC CGGAGGGGAC GGATTTTGAC 
AACCCCATGC AGAGATCACG GAAATGGCAG CGGCGATTCT TCGTCCTGTA TGAGCACGGC 
TGCTTGCGCT TCGCTCTGGA TGAATCGCCC AGCACGCTGC CGCAGGGAAC GGTGAACATG 
AACCAGTGCA CAGACGTGGT GGACGCGGAG CCGCGCACGG GCCAGAAGAA CGCGCTGTGC 
ATCGTGACGC CCGAGCAGGA GTATTTCATC CGCGGAGACA ATAAAGAGAT CATCAACGGA 
TGGAGTGAGC AGCTAGCGGT GTATCCCAGA ACCAACAAGC AGAACCAGAA GAAGAAGCGC 
AAGGTGGAGC CGGCCACCTC ACAGGAGCCG GGTCCGGCTA AGGTGGCGGT GACGGGCGCC 
GGCATCCCTG ACGCAGAGAA AACTCCAGAC TCCAGCTCCA TCATCTGGCA GGAGGAGCTG 
CAGAGCAGAG AAGCAGACGC CGCTCACACC TGGAGCAGCG AGATTCCTCC TGTCGGCTCA 
CCGCTGCCCT CTACAGGCGA GGGGTCGACG GTGAGCTCAG TGAATGGGGA TGAGGTTGAC 
CGCTGCGGAC TTTCCTTCCA CTCGCCGGTC TCTCAGGCTC CTGTAGACCC GCTGTCCCCG 
ACAGGCAGCA GCTCCAGCAG GCAGAGTGCA GAGACGGGCG GGGTCCCTCG CTGCCTATCC 
CCGGCTCCCA GCGACCCCTT CCCCTCAGGC AGCAGCCTCC TGTCCAACGG CTCGCACATC 
AGCGGCTCTA TGAGCTCTCT GGACTCGGAG GCTAGCGGCA GCACCGTCAC GAGCAGCGAC 
AGCCACCCGC CGGAGCCCCG CGCCCTCAAA CGAAGCCGCC TACGGAGCCC ACAGACCCGG 
GAGCGGGAGC TAGTGCTGAG CCCTGAGAGA AGCGGCCGCT CGAATGTGAT TGAGAAGCTG 
GAGGCGCTGG AGCTGGAGCA GCAGGAGCGC ATGGAGGTTC AGGAGTCCAG CAGCACTGAG 
GCCCGACAGG GGCGGAGTCA GAACAGGAGA TTCCAGCGGC AGGAGCAGAA GACGGACGCA 
GCACAGGCTC TAGACTTCCA TCCTCCTCTA AGAAGAGCCA AATCTCTGGA CCGCAGGACC 
ACTGAGTCCG TCATGACCCC TGATCTGCTG AACTTTAAGA AAGGCTGGAT GGTGAAGCTG 
GATGAAGAAG GACAGTGGAA GAAGTATTGG TTTGTGCTGA CGGATCACAG TCTGCGATAC 
TACAAGGACT CCATCGCTGA AGAGGCGTCA GATCTGGATG GAGAGATCGA CCTCAGCACA 
TGCTATAATG TTACAGAGTA TCAGGCGCAG AGGAACTACG GCTTCCAGAT TTACACTCAG 
GAGGCGGTGC ACACACTCTC CGCCATGACT GCAGGAATCC GCCGTAACTG GATCCAGGCA 
GTGATGAAGA ATGTCCGGCC AGCCACTGCT CCAGATGTAG CCAGTCTGAC GGATGAGCAT 
GGGTCGTTTG AGGGTGTCTG TCGACCGGAC GTGACGCAGG ACTCTCCCTC CTCTGAGGCG 
TCTGTGGAGC GGGAAACAGG GGCCAAAAAA AGCCGTGCGC GGGAACGCAG GCGAGAGGGG 
CGGAGCAAAA CTTTCGACTG GGCGGAGTTT CGTCCCATCG CTCAGGCATT GGCTCAGCAG 
AGAGCGCAGG AGGCGGAGCT TACAGAGCGC AGCAAGAGGA GAGAGGAGCG AAGGCGGAGG 
TATGAGAGCG TGACCGGAGC AACCTTTGAT CCTGCACTGA TGGAGCAAGA GGGGGGCGTG 
GCTCAGACTG AAGCTCCGCC CCTGTCGGCT GAATGGCAGC AGCGAGTGGA GGATGAGATC 
GAGCAACGCT GGCAGCAGGT GGAGAAAACA CCAATCAGAG ACGAGCGCAG GGTTCCGTTA 
GGCTCCGCCC ACAGCAGAGA CGCCACAGAC ATGGAGAAGC TGCTGGAGGG ATACAAGAGA 
GGGGTGGAGG AGCTCAAGGC TCAGCTGGAG AACTGCCATC AGCAGCTTCT GGACTCCAAT 
CGGCACAAGC AGGATCTGGA GGGAAAACTG AAGATGGCGG TGGAGCGCGA GCAGCAGACG 
CGAGCCGGGT ACATCTCTCC GCTGGAGTCC CCTTTGAGTG TTGAGACAGA GCCGCAGATG 
AAGAGGACTG AACTGGTTAG TTCTCAAGCT CAGAGTTTAA CAAAGAAGTA CCAGGAAACC 
AAAGAGCTCC TGCAGCTGCA AGAACTGAAA AAGCGCGACA TGCAGGCACA GCTTGGCCTT 
TCTCTCTCAC ACTGGCCCGC AAAGGACCCT TCGCTTTCTG ACACCCAAAA ACACGAGGTC 
TGTCCCTCGA AGTGCACTCG GCGAACGCTC ACGCTTACTC TTCAGGATAG CGAGGGAACG 
ATTCGAGAGC TTGAGCACTT AATAAACACT GACGATCCTT TAACGTTGAG GGAGTTTATA 
AAGCTCGTCC AGTTGCATAG TGAACATGTT TGCGTGGTAG AATCTCCTCC AGGGATGGAG 
CAAAGTGAAC ACAGCCGCTC AGTTGGAGCG GAGTGCCAGA AACTGCTGGA AAGCTTGAAG 
AACCAGCAGG AGATGGAGAG CGAAGCTGTC AGGAGAAGTT TGGAAAAGGC TGGAGATTGC 
ATTCGAGACT ATGAAGCGCG TTTGCTCACT ATGGAGGACA TGCTAGGACG AGCTCAGCAG 
CAAAAAGCGG AGATTCTCCT GCAGTCCCCA TCGAGAGTAG AAGAGTTCAG CGCTGATATG 
AGCAGCAGAG ATCTGTCGCA GCAGGTGGGG ATTCTGGCCA ATGAAAATGT AGCGTTGAAT 
CAACGCTATC AGGAGATCGT GAACCAACTG AGAGAAGCAG ATCGAGAGAT AGACAGGCTG 
AAGGCGGAGG TGTATAGACT GCGACACAGT AAGGAAGACG ATGAAGAGTC CGCAGATAAG 
AGAGAGCTCT GCGAAAAATC CCACAAGCTT CAAGAAGCTC TGATTAAATT AGAGACGCTT 
GGTAGTAATC TGAAAGACAC TGAGAAAAGA TTGCAGCTAA AAGAAGCCAC TCTTCGTGGA 
CTTGGATTTC AGGTGGCGGA AAGTGAAGCT GAAGACGATC TCAAGTGTCA GTTGGAGATC 
CTTCAATGCA AGCTTTCTGA GAAAGAGAAG ATGTGCGAAG AACTCCAGTC GCAGAATCCA 
AGACAAGAGG ACGCGGTGAG GCTCAATCAG ATGCTTGTAG AAGCTCAGGA GGAGATCGCA 
GGGTTGAAAC GGAAGCTAGA AAGTCGAGCA GGGTTCAGTA GCGAAAGTGA GTTTATGGAA 
GAGGAAGGTG CGAGTGAGTT AAAGAGAAAG TCAGAGTCTC TAATGCTTGT GTTTGGTGCG 
TTAGTAAACG GCAGGGAGGC AGAATGCGAT GCGTTTATTG TGGATGAGGA TCACGCAGGA 
TTGATGACGG AGAGAAGGAT CTTATGCAGG ATTGTAGACG GTCTGCAGAA AGACGAGCAA 
TCTGTGAGGC AAGTGGTAGA GCGAATGATG GAGGATAATA ACATGCTGAT TCATGCAAAC 
AAGCTCTCCG ACATTCAACC AGGAGAGACG ATGCATGATG AAGATGACGC AGACATCATG 
AAGAGTCTCT CTGAAGCGGT TCAGAGCAAA GTGGCGTTAT TAAATCAGAC TTCTTTAGCG 
AGAACGGGTG ACAAACTCAC TTTGAGAAGC AGATCTGACT ACATACGTGA AGCAATCACA 
GACATAACAC ACATGTACCA AACGCTCAGG CTGAAGAAAG AGTTTGCACG TGCTGAAATG 
AGGACGCAAC AGGAATTAGC AGTGACGCGC ATTCATATCG AAGGAGAGCC GATCGACTCT 
TTGGATAAAA GCATTCAGCT GCAGGATCTG ATGGCGAGAC ATAAGAAGGA GCTTCGAGAG 
CTGAAGGAGG TTTACGAGAC GGATGTGGAA AAGCTTAAAA AGGAGTTGGA GAAAGCCGGA 
GAAACCCTGA AGATGAGATC TGAAGAGAAC GTCCGAGAGA TCGACTCGCT GACCGTGTGC 
ATGGAGAACC TGGAGAAAAA GCACGAAGCC GAAAGGCGAG AATTACAGAC ACGCTTCAGC 
CTGCAAATGG AGGAGCTCTG GGAAGCTGTC GGGTTTGAGG ATAAAGATTG GAGTTCGGGA 
TCCCTCAAAG AGCAAATCCT GAAGCTGGTG CGCCGAGAGT CTGAACTGAC CCAGCTAATG 
CATCAGAAAG ATGGAGACCT GCGTCTGAGA TACGAGAAAG ATCTGGAAAA GCTAAAGGCC 
ACATGTGAGC GAGGTTTCGC CGCTATGGAG GAGTCGCACC AGAAGGTGAT CGAGGAGCTG 
CAGAAGAAAC ACCAGAGAGA GCTGGAGAAG CTGCAGGAGG AGAAGGAGAG ACTGCTGGAG 
GAGGAGACGG CTGCTACCAT CGCCGCTATC GAGGCGATGA AGAACGCACA CCGGTCAGAG 
CTGGAGCGAG AGCTGGAGAA AGCACGCAAA GCCACCAGCA GCACAGAGAA CGCAGACATC 
GAGGAGATCC ACAAACAGCA CGAGGAGGAG CTGGTGTCGT ATCAGCGGGA GATCGAGGTG 
CTGTCAGAGC AGTATTCACA GAAGTGTCTG GAGAACGCTC ATCTGGCTCA GGCGCTGGAG 
GCCGAGCGGC AGGCGCTCAG ACAGTGCCAG AGGGAGAACC AGGAGCTCAA TGCACACAAC 
CAGGAGCTCA ACAATCGTCT GGCTGCAGAG ATCACCAAGA TGCGCTCGAT GGCGACTGAA 
GACGGAGCTG ACACTGGAGG ACTGATTCAA GGGAAGGAGC TGTACGAGCT GGAGGTGATG 
CTGAGGGTGA AGGAGTCAGA AGTGCAGTAT CTGAAGCAGG AGATCAACTC TCTGAAAGAT 
GAGCTCCAGA CGGCACAAAG AGATAAGAAA TATGCAACAG ATAAATACAA GGACATCTAC 
ACTGAGCTGA GCATCGTGCG GGCCAAAGCG GAGCGGGATC TGGGCCGCCT GCGGGAGCAG 
CTGCAGCTGG CCCACAATGC ACTGGGGGAG CCGACGCTGG AGGACATGGA GCGCGGAGGA 
TACGATATCA TGAAGTCCAA GAGCAACCCG GATATATTAA AGATGGCGGC AGCAGCGGCA 
AAACGCTCCG AGAGGACAAT GAGGTCAAAG TCTGTGAGTC GTGATGTGCC CTGCGACAGC 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021335872.1
CDS	680..5962
Translation	MSSAKENTCRKFQANIFNKSKCQNCFKPRELHLLTDQDLNQAKPIYGGWLCLAPEGTDFDNPMQRSRKWQRRFFVLYEHGCLRFALDESPSTLPQGTVNMNQCTDVVDAEPRTGQKNALCIVTPEQEYFIRGDNKEIINGWSEQLAVYPRTNKQNQKKKRKVEPATSQEPGPAKVAVTGAGIPDAEKTPDSSSIIWQEELQSREADAAHTWSSEIPPVGSPLPSTGEGSTVSSVNGDEVDRCGLSFHSPVSQAPVDPLSPTGSSSSRQSAETGGVPRCLSPAPSDPFPSGSSLLSNGSHISGSMSSLDSEASGSTVTSSDSHPPEPRALKRSRLRSPQTRERELVLSPERSGRSNVIEKLEALELEQQERMEVQESSSTEARQGRSQNRRFQRQEQKTDAAQALDFHPPLRRAKSLDRRTTESVMTPDLLNFKKGWMVKLDEEGQWKKYWFVLTDHSLRYYKDSIAEEASDLDGEIDLSTCYNVTEYQAQRNYGFQIYTQEAVHTLSAMTAGIRRNWIQAVMKNVRPATAPDVASLTDEHGSFEGVCRPDVTQDSPSSEASVERETGAKKSRARERRREGRSKTFDWAEFRPIAQALAQQRAQEAELTERSKRREERRRRYESVTGATFDPALMEQEGGVAQTEAPPLSAEWQQRVEDEIEQRWQQVEKTPIRDERRVPLGSAHSRDATDMEKLLEGYKRGVEELKAQLENCHQQLLDSNRHKQDLEGKLKMAVEREQQTRAGYISPLESPLSVETEPQMKRTELVSSQAQSLTKKYQETKELLQLQELKKRDMQAQLGLSLSHWPAKDPSLSDTQKHEVCPSKCTRRTLTLTLQDSEGTIRELEHLINTDDPLTLREFIKLVQLHSEHVCVVESPPGMEQSEHSRSVGAECQKLLESLKNQQEMESEAVRRSLEKAGDCIRDYEARLLTMEDMLGRAQQQKAEILLQSPSRVEEFSADMSSRDLSQQVGILANENVALNQRYQEIVNQLREADREIDRLKAEVYRLRHSKEDDEESADKRELCEKSHKLQEALIKLETLGSNLKDTEKRLQLKEATLRGLGFQVAESEAEDDLKCQLEILQCKLSEKEKMCEELQSQNPRQEDAVRLNQMLVEAQEEIAGLKRKLESRAGFSSESEFMEEEGASELKRKSESLMLVFGALVNGREAECDAFIVDEDHAGLMTERRILCRIVDGLQKDEQSVRQVVERMMEDNNMLIHANKLSDIQPGETMHDEDDADIMKSLSEAVQSKVALLNQTSLARTGDKLTLRSRSDYIREAITDITHMYQTLRLKKEFARAEMRTQQELAVTRIHIEGEPIDSLDKSIQLQDLMARHKKELRELKEVYETDVEKLKKELEKAGETLKMRSEENVREIDSLTVCMENLEKKHEAERRELQTRFSLQMEELWEAVGFEDKDWSSGSLKEQILKLVRRESELTQLMHQKDGDLRLRYEKDLEKLKATCERGFAAMEESHQKVIEELQKKHQRELEKLQEEKERLLEEETAATIAAIEAMKNAHRSELERELEKARKATSSTENADIEEIHKQHEEELVSYQREIEVLSEQYSQKCLENAHLAQALEAERQALRQCQRENQELNAHNQELNNRLAAEITKMRSMATEDGADTGGLIQGKELYELEVMLRVKESEVQYLKQEINSLKDELQTAQRDKKYATDKYKDIYTELSIVRAKAERDLGRLREQLQLAHNALGEPTLEDMERGGYDIMKSKSNPDILKMAAAAAKRSERTMRSKSVSRDVPCDS

Target ORF information:

RefSeq Version	XM_021480197.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio myosin phosphatase Rho interacting protein (mprip), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021480197.1

ORF Insert Sequence:

ATGTCGAGCG CGAAGGAAAA CACGTGTAGG AAATTTCAGG CGAACATTTT CAACAAAAGC 
AAGTGTCAGA ACTGCTTCAA GCCGCGAGAG CTGCATCTGC TGACGGATCA GGATCTCAAT 
CAGGCCAAGC CCATCTATGG TGGATGGCTG TGTTTGGCTC CGGAGGGGAC GGATTTTGAC 
AACCCCATGC AGAGATCACG GAAATGGCAG CGGCGATTCT TCGTCCTGTA TGAGCACGGC 
TGCTTGCGCT TCGCTCTGGA TGAATCGCCC AGCACGCTGC CGCAGGGAAC GGTGAACATG 
AACCAGTGCA CAGACGTGGT GGACGCGGAG CCGCGCACGG GCCAGAAGAA CGCGCTGTGC 
ATCGTGACGC CCGAGCAGGA GTATTTCATC CGCGGAGACA ATAAAGAGAT CATCAACGGA 
TGGAGTGAGC AGCTAGCGGT GTATCCCAGA ACCAACAAGC AGAACCAGAA GAAGAAGCGC 
AAGGTGGAGC CGGCCACCTC ACAGGAGCCG GGTCCGGCTA AGGTGGCGGT GACGGGCGCC 
GGCATCCCTG ACGCAGAGAA AACTCCAGAC TCCAGCTCCA TCATCTGGCA GGAGGAGCTG 
CAGAGCAGAG AAGCAGACGC CGCTCACACC TGGAGCAGCG AGATTCCTCC TGTCGGCTCA 
CCGCTGCCCT CTACAGGCGA GGGGTCGACG GTGAGCTCAG TGAATGGGGA TGAGGTTGAC 
CGCTGCGGAC TTTCCTTCCA CTCGCCGGTC TCTCAGGCTC CTGTAGACCC GCTGTCCCCG 
ACAGGCAGCA GCTCCAGCAG GCAGAGTGCA GAGACGGGCG GGGTCCCTCG CTGCCTATCC 
CCGGCTCCCA GCGACCCCTT CCCCTCAGGC AGCAGCCTCC TGTCCAACGG CTCGCACATC 
AGCGGCTCTA TGAGCTCTCT GGACTCGGAG GCTAGCGGCA GCACCGTCAC GAGCAGCGAC 
AGCCACCCGC CGGAGCCCCG CGCCCTCAAA CGAAGCCGCC TACGGAGCCC ACAGACCCGG 
GAGCGGGAGC TAGTGCTGAG CCCTGAGAGA AGCGGCCGCT CGAATGTGAT TGAGAAGCTG 
GAGGCGCTGG AGCTGGAGCA GCAGGAGCGC ATGGAGGTTC AGGAGTCCAG CAGCACTGAG 
GCCCGACAGG GGCGGAGTCA GAACAGGAGA TTCCAGCGGC AGGAGCAGAA GACGGACGCA 
GCACAGGCTC TAGACTTCCA TCCTCCTCTA AGAAGAGCCA AATCTCTGGA CCGCAGGACC 
ACTGAGTCCG TCATGACCCC TGATCTGCTG AACTTTAAGA AAGGCTGGAT GGTGAAGCTG 
GATGAAGAAG GACAGTGGAA GAAGTATTGG TTTGTGCTGA CGGATCACAG TCTGCGATAC 
TACAAGGACT CCATCGCTGA AGAGGCGTCA GATCTGGATG GAGAGATCGA CCTCAGCACA 
TGCTATAATG TTACAGAGTA TCAGGCGCAG AGGAACTACG GCTTCCAGAT TTACACTCAG 
GAGGCGGTGC ACACACTCTC CGCCATGACT GCAGGAATCC GCCGTAACTG GATCCAGGCA 
GTGATGAAGA ATGTCCGGCC AGCCACTGCT CCAGATGTAG CCAGTCTGAC GGATGAGCAT 
GGGTCGTTTG AGGGTGTCTG TCGACCGGAC GTGACGCAGG ACTCTCCCTC CTCTGAGGCG 
TCTGTGGAGC GGGAAACAGG GGCCAAAAAA AGCCGTGCGC GGGAACGCAG GCGAGAGGGG 
CGGAGCAAAA CTTTCGACTG GGCGGAGTTT CGTCCCATCG CTCAGGCATT GGCTCAGCAG 
AGAGCGCAGG AGGCGGAGCT TACAGAGCGC AGCAAGAGGA GAGAGGAGCG AAGGCGGAGG 
TATGAGAGCG TGACCGGAGC AACCTTTGAT CCTGCACTGA TGGAGCAAGA GGGGGGCGTG 
GCTCAGACTG AAGCTCCGCC CCTGTCGGCT GAATGGCAGC AGCGAGTGGA GGATGAGATC 
GAGCAACGCT GGCAGCAGGT GGAGAAAACA CCAATCAGAG ACGAGCGCAG GGTTCCGTTA 
GGCTCCGCCC ACAGCAGAGA CGCCACAGAC ATGGAGAAGC TGCTGGAGGG ATACAAGAGA 
GGGGTGGAGG AGCTCAAGGC TCAGCTGGAG AACTGCCATC AGCAGCTTCT GGACTCCAAT 
CGGCACAAGC AGGATCTGGA GGGAAAACTG AAGATGGCGG TGGAGCGCGA GCAGCAGACG 
CGAGCCGGGT ACATCTCTCC GCTGGAGTCC CCTTTGAGTG TTGAGACAGA GCCGCAGATG 
AAGAGGACTG AACTGGTTAG TTCTCAAGCT CAGAGTTTAA CAAAGAAGTA CCAGGAAACC 
AAAGAGCTCC TGCAGCTGCA AGAACTGAAA AAGCGCGACA TGCAGGCACA GCTTGGCCTT 
TCTCTCTCAC ACTGGCCCGC AAAGGACCCT TCGCTTTCTG ACACCCAAAA ACACGAGGTC 
TGTCCCTCGA AGTGCACTCG GCGAACGCTC ACGCTTACTC TTCAGGATAG CGAGGGAACG 
ATTCGAGAGC TTGAGCACTT AATAAACACT GACGATCCTT TAACGTTGAG GGAGTTTATA 
AAGCTCGTCC AGTTGCATAG TGAACATGTT TGCGTGGTAG AATCTCCTCC AGGGATGGAG 
CAAAGTGAAC ACAGCCGCTC AGTTGGAGCG GAGTGCCAGA AACTGCTGGA AAGCTTGAAG 
AACCAGCAGG AGATGGAGAG CGAAGCTGTC AGGAGAAGTT TGGAAAAGGC TGGAGATTGC 
ATTCGAGACT ATGAAGCGCG TTTGCTCACT ATGGAGGACA TGCTAGGACG AGCTCAGCAG 
CAAAAAGCGG AGATTCTCCT GCAGTCCCCA TCGAGAGTAG AAGAGTTCAG CGCTGATATG 
AGCAGCAGAG ATCTGTCGCA GCAGGTGGGG ATTCTGGCCA ATGAAAATGT AGCGTTGAAT 
CAACGCTATC AGGAGATCGT GAACCAACTG AGAGAAGCAG ATCGAGAGAT AGACAGGCTG 
AAGGCGGAGG TGTATAGACT GCGACACAGT AAGGAAGACG ATGAAGAGTC CGCAGATAAG 
AGAGAGCTCT GCGAAAAATC CCACAAGCTT CAAGAAGCTC TGATTAAATT AGAGACGCTT 
GGTAGTAATC TGAAAGACAC TGAGAAAAGA TTGCAGCTAA AAGAAGCCAC TCTTCGTGGA 
CTTGGATTTC AGGTGGCGGA AAGTGAAGCT GAAGACGATC TCAAGTGTCA GTTGGAGATC 
CTTCAATGCA AGCTTTCTGA GAAAGAGAAG ATGTGCGAAG AACTCCAGTC GCAGAATCCA 
AGACAAGAGG ACGCGGTGAG GCTCAATCAG ATGCTTGTAG AAGCTCAGGA GGAGATCGCA 
GGGTTGAAAC GGAAGCTAGA AAGTCGAGCA GGGTTCAGTA GCGAAAGTGA GTTTATGGAA 
GAGGAAGGTG CGAGTGAGTT AAAGAGAAAG TCAGAGTCTC TAATGCTTGT GTTTGGTGCG 
TTAGTAAACG GCAGGGAGGC AGAATGCGAT GCGTTTATTG TGGATGAGGA TCACGCAGGA 
TTGATGACGG AGAGAAGGAT CTTATGCAGG ATTGTAGACG GTCTGCAGAA AGACGAGCAA 
TCTGTGAGGC AAGTGGTAGA GCGAATGATG GAGGATAATA ACATGCTGAT TCATGCAAAC 
AAGCTCTCCG ACATTCAACC AGGAGAGACG ATGCATGATG AAGATGACGC AGACATCATG 
AAGAGTCTCT CTGAAGCGGT TCAGAGCAAA GTGGCGTTAT TAAATCAGAC TTCTTTAGCG 
AGAACGGGTG ACAAACTCAC TTTGAGAAGC AGATCTGACT ACATACGTGA AGCAATCACA 
GACATAACAC ACATGTACCA AACGCTCAGG CTGAAGAAAG AGTTTGCACG TGCTGAAATG 
AGGACGCAAC AGGAATTAGC AGTGACGCGC ATTCATATCG AAGGAGAGCC GATCGACTCT 
TTGGATAAAA GCATTCAGCT GCAGGATCTG ATGGCGAGAC ATAAGAAGGA GCTTCGAGAG 
CTGAAGGAGG TTTACGAGAC GGATGTGGAA AAGCTTAAAA AGGAGTTGGA GAAAGCCGGA 
GAAACCCTGA AGATGAGATC TGAAGAGAAC GTCCGAGAGA TCGACTCGCT GACCGTGTGC 
ATGGAGAACC TGGAGAAAAA GCACGAAGCC GAAAGGCGAG AATTACAGAC ACGCTTCAGC 
CTGCAAATGG AGGAGCTCTG GGAAGCTGTC GGGTTTGAGG ATAAAGATTG GAGTTCGGGA 
TCCCTCAAAG AGCAAATCCT GAAGCTGGTG CGCCGAGAGT CTGAACTGAC CCAGCTAATG 
CATCAGAAAG ATGGAGACCT GCGTCTGAGA TACGAGAAAG ATCTGGAAAA GCTAAAGGCC 
ACATGTGAGC GAGGTTTCGC CGCTATGGAG GAGTCGCACC AGAAGGTGAT CGAGGAGCTG 
CAGAAGAAAC ACCAGAGAGA GCTGGAGAAG CTGCAGGAGG AGAAGGAGAG ACTGCTGGAG 
GAGGAGACGG CTGCTACCAT CGCCGCTATC GAGGCGATGA AGAACGCACA CCGGTCAGAG 
CTGGAGCGAG AGCTGGAGAA AGCACGCAAA GCCACCAGCA GCACAGAGAA CGCAGACATC 
GAGGAGATCC ACAAACAGCA CGAGGAGGAG CTGGTGTCGT ATCAGCGGGA GATCGAGGTG 
CTGTCAGAGC AGTATTCACA GAAGTGTCTG GAGAACGCTC ATCTGGCTCA GGCGCTGGAG 
GCCGAGCGGC AGGCGCTCAG ACAGTGCCAG AGGGAGAACC AGGAGCTCAA TGCACACAAC 
CAGGAGCTCA ACAATCGTCT GGCTGCAGAG ATCACCAAGA TGCGCTCGAT GGCGACTGAA 
GACGGAGCTG ACACTGGAGG ACTGATTCAA GGGAAGGAGC TGTACGAGCT GGAGGTGATG 
CTGAGGGTGA AGGAGTCAGA AGTGCAGTAT CTGAAGCAGG AGATCAACTC TCTGAAAGAT 
GAGCTCCAGA CGGCACAAAG AGATAAGAAA TATGCAACAG ATAAATACAA GGACATCTAC 
ACTGAGCTGA GCATCGTGCG GGCCAAAGCG GAGCGGGATC TGGGCCGCCT GCGGGAGCAG 
CTGCAGCTGG CCCACAATGC ACTGGGGGAG CCGACGCTGG AGGACATGGA GCGCGGAGGA 
TACGATATCA TGAAGTCCAA GAGCAACCCG GATATATTAA AGATGGCGGC AGCAGCGGCA 
AAACGCTCCG AGAGGACAAT GAGGTCAAAG TCTGTGAGTC GTGATGTGCC CTGCGACAGC 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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1-732-210-0262

mprip cDNA ORF clone, Danio rerio(zebrafish)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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