nhsb cDNA ORF clone, Danio rerio(zebrafish)

The following nhsb gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the nhsb cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
ODa148024 XM_021479857.1
Latest version!
Danio rerio Nance-Horan syndrome b (congenital cataracts and dental anomalies) (nhsb), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $881.30
$1259.00
ODa148025 XM_021479858.1
Latest version!
Danio rerio Nance-Horan syndrome b (congenital cataracts and dental anomalies) (nhsb), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $881.30
$1259.00
ODa148026 XM_021479859.1
Latest version!
Danio rerio Nance-Horan syndrome b (congenital cataracts and dental anomalies) (nhsb), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $881.30
$1259.00
ODa148027 XM_021479860.1
Latest version!
Danio rerio Nance-Horan syndrome b (congenital cataracts and dental anomalies) (nhsb), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $881.30
$1259.00
ODa43148 XM_009306118.3
Latest version!
Danio rerio Nance-Horan syndrome b (congenital cataracts and dental anomalies) (nhsb), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $881.30
$1259.00
ODa43149 XM_009306119.3
Latest version!
Danio rerio Nance-Horan syndrome b (congenital cataracts and dental anomalies) (nhsb), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $881.30
$1259.00
ODa43150 XM_009306120.3
Latest version!
Danio rerio Nance-Horan syndrome b (congenital cataracts and dental anomalies) (nhsb), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $881.30
$1259.00
View Old Accession Versions >>
ODa43149 XM_009306119.2 Danio rerio Nance-Horan syndrome b (congenital cataracts and dental anomalies) (nhsb), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $881.30
$1259.00
ODa43148 XM_009306118.2 Danio rerio Nance-Horan syndrome b (congenital cataracts and dental anomalies) (nhsb), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $881.30
$1259.00
ODa43150 XM_009306120.2 Danio rerio Nance-Horan syndrome b (congenital cataracts and dental anomalies) (nhsb), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $881.30
$1259.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID ODa148024
    Clone ID Related Accession (Same CDS sequence) XM_021479857.1
    Accession Version XM_021479857.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4893bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product Nance-Horan syndrome protein isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007122.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    ATGCCTTTTG CCAAACGGAT CGTTGAGCCC CAGCTGCTGG TTAGACATCC CATCCCTAAT 
    GATGAAGGGT TACTGTTCGA GGATCTGTGC TCCATCACCA ATGTGGCTCT ATCCCGGACC
    CTGCGGCAGC TCTCCGACCT GTCCAAACAC GCTTGCTCCT TATTTCAGGA GCTTGAGAAC
    GAAATAGTGT CCACAAACCA ACGGGTTTGG GCTTTGCAGA ACAAAATAGG CAAAATTCAG
    CAAACCGCCA GTGCGCTGGA TCCAAAAATG GAGGCTGTGC GTTTCAGGAG CATCTCTATA
    GCGGTCTCGA ATCTGGACAT TGAGAGTAAA CTGACGTCGC ACTATCAGGC TCCATGGCAT
    CAGCAACACA ATCTGTTCCA CACTTGCACA CGGCCAGCGT GTCTGGAGGA GCTGCACCGA
    CATGCCAAGC TCAACCTGCG GGCGCTGCAC AGAGACCAGC AGCAGCGTCA GCGTTCAACC
    AGCAGAGAGC GCAGTCGGGT CACTATATCA ATATCTGTGG CACCGCCAAT GCCAACCTTC
    CCTTCAACTC ACAAACTCAG ACGGCGAGAG CAGAGAGGTC GACATTCACG CATGGAGAGG
    AGCGTTAGAG AGAGTGATGT GCAAACTATC CAGAGGAAGG AGAGACCAGC CAAGGATTCT
    GAATTCAAAC CTGTCCTCAG AAAGTATCAG CGCTCCCGCT CCCCCTCCCC TGTTCAGTGT
    TGTTACTTCA TTCCCTGGAT TAGAAAGGCT GGAACCAACA CAGAAACGGA TGGAGAACTA
    CAAGTGATGA GCCATAGGCC TAAATGTCCT GTTCCCAATG CACCTACCAC CCTGGACAAA
    CAGACTAACT GGTCTAAAGC CCTGCCTCTT CCCACTCCAG AAGAAAGAAT AAAGAATGAC
    TCTCAAGTGA TTTCATCCTG TATCATTCCA ATAAATGTTT CGGGTGTTGG ATTTGACAGA
    GAAGCCAGTG CTCGCTGCTC TCTTGTTCAC TCGCAATCGG TTCTACAGAG ACGGCGGAAA
    CTCAGGAGGC GAAAAACGAT CACTGGGATC CCCAAACGAG TTCAACAAGA CATGGATTCT
    GACGAATCCC CTGTTGCTAG AGAAAGAACA GTGATAATCC ACGCCAATCC ACACAAGTCC
    CACGAGTGGC ATGAGGAGCT CTCCTTGAGT GGCAGAGTAT TGCATACTAA GGATTCGGGC
    TGTCAGACAG ATGACTTCTT GATTACTGCT CCATCCCGAA GACGCATCCG TGCCCAAAGA
    GGTCAGGGAA TTCCAGCGTC TCTCTCTCAC TCCACTGGAA ACATTACCTC CCTCCCGGAT
    CGTTCTGATA CGGTTTATGC TGCAGCATCA GCCACTCGTG TCCGTTCTAG GAGCCTTCCA
    CGTGAGGGTG GTCGGCTTCT GGATGAGGAC CAAGATGACA GTGATGATGA TGATGATGAA
    GATGATGATG AAGATGATGA AGAGGAAGAT GAGGAGCTCT CTCCATATGA AGCCGAAGAC
    TTCCTGCCAG GTCCCGGACA GAGAATTCCA AAGGATGAGG AAGAGAGTAC TGACGACCAA
    GCCATGCCAG AGCTACAGTT TGGAAGTCTT AAGCGTATGC AGCATTCAGA GAGCCCTGAC
    CACACATGGA TTGAGAGAGG CAGATCCAGA CTCCCACGGA AAGTAGACAT GGGGAGCTGT
    GAGATTTCCT CTAGTTCAGA TACTTTCAGC AGCCCCATAC ACTCTGCATC CACTACAGGA
    GTGCTTGGCA GCCAGATTGA CCATAAAGAG GATCACCAGT CCTCAAGTGG CAACTGGAGT
    GGAAGCAGCT CCACTTGCCC ATCGCAGACA TCCGAGACTC TTCCACCTGC TGCCTCCCCT
    CCCTTGACGG GATCCTCACA CTGTGATTCA GAACTTTCCC TGAATACTGG GTCCCATGTC
    ATAGATGACA ACACTGGTTT CATAATTGAT CCCTATCACA TAGACAAGCC ACAGGGACAG
    GGGCAACGAT CCAATTCATT TACCTCATCA GCTACTGATC AGATGGATGA TGCAGGGGTC
    AGCACTGCTA GTGATGGGGA GTGGAACTGT GCCCAGGACC AGCAGGATCA GTCCTGCACC
    CAAGACTTCA GCCCAAAGCG TTCCAGAGAG GATGAGAGTG GTGTCAGCTG CCCTAGTTAT
    TCTAGTGGGG AAACTTACGA GAGCTTCAGT GACCAAGCAT CCGCTAGAAA ATCTGAGATG
    CACTACATTG TCGACACTGA AGGATTCTAT TCCTCCATGC ACTTTGACTC TGGTCTTAAG
    GGTAGCAGAA GCTACTACAA CTATGCAGCC ATTGACCCCG ACTGTGGCCC AAGTGGTAAT
    ATAGCACCTG GCATGACTGA ATACCCTCAG CCAGAGTACA GAGCCACCAG GACACTGGGC
    AGACCCTGTC TCTCCTTAAG AAAACCAAAG GCCAAGCCAC CCCCACCAAA ACGTAGCTCT
    TCATTAAAGG AAACAAGCAG TGATGTGAAC GTTCCAGAGC AGAACGAACC AAAGATTACT
    TGTGAGTTGC CACTGCCCTT GTCTTCCAGG GAGATGAAAC TGCAGCTGGA CTTGGCTGAT
    TCTGCAGGGC ACCTTGAGAC TGCAGGTCTT GAATCACTTG GTGCATGGGG AGTGGAAAAT
    GTTAGGGACA TGCTTGACTG CTCCTCCCCA TTCAGTTCCT CAGACACACA TTCCTTCAAA
    GATGAAGGTG CTGTGCAAGC AGACTATGCA GACCTCTGGC TTCACAATGA CTTGAAATCA
    AATGATCCTT ATCGGTCCCT CTCTAACTCT AGCTCTGCTA CAGGTACAAC TGTTATTGAA
    TGCATCAAGT CACCAGAAAG ATCAGAAACG CACACCTGTC AACCCAGGTC CAGACCTTCT
    TCCCCAACTC TTCCTCCACC TGAAAGTGAA TTTAAGCTTG CTTCTCCCGA AAAGCTGGCA
    GGCTTGGCGT CCCCTTCCAG TGGATATTCC AGTCAGTCTG AAACACCTAC ATCTTCCTTC
    CCCTCTGCTT TCTTCCCAGG GCCCCTGTCT CCAACCAGTG GGAAGAGGAA GCCCAAAGTC
    CCTGAAAGGA AGTCCTCACT TTGTTCCTTA CAGCAGCAAC AGCTTTCAGT CAGAGACCCA
    GGCATTTCCT GTAGGAGAGA AACTGACTTC TATGCCATAC CCCCAAGTCA CCTTGACCTA
    AGTGCTCTTC ACAGTAAGCA CTTTCCTCAC AGAAATCAAA TGCACATCCT CCACCAGAAT
    AAGCAGAAAG CTGCCATAGC AGCAGCTGCT GCAGCAGAAG CTCGCAGTGC AGCAGCTGCT
    GCAGCAGAAG CTCGCAGTGC AGCAGCTGCT GCAGCAGAAG CTCGCAGTGC AGCAACTGCA
    GCCGCTGCTG CCGAGGCCTG CACTGCAGCT ACAGCTTCTA CTAAAGAGAG TGCAACAAAT
    ACAGCACCCA TCTCAGCCCA TTTGGCTATT ACTCCAACGG TTCTTAGATC AGTGCAACTG
    CGATCTATAT GTAGACCATC TGATGGCAAC CAAGGGCTTG ATCAAGACAG TTCAAATCTC
    ATAACTCGTC CTAAGTGTCC CACAATAATA ACTGATGCCC CATCATCTAG CAACAGGCAC
    AACAGGAAGC CACCAGCCTA CAAACCCCCT GCTGCACAGG TTTGTGATTC ACAGATTCCA
    CTGGTGGAAA ACATTGTTTT TCCACAAGAA GAAGTGAGAG TGAGACAGGA GAGGTTTGGT
    CCTGGTACTT ACTGGGCAAT GACTGCTTTC AGAACTCCTG TGGACCCTAA TTGTGAAAAA
    GAAGTCTCTT TAAAAAGGTC ATCTCAGTGT TTTCAGCAAG AACAAGACAG CAGAAGGTGC
    CTAGATGTGC AAAAGACATT GTCACCAGAG AGACTAAAAC AAGATTTGAA TCAACTATCG
    CGGCCAGACC CTTCAACACA GTCCATGTGT TTGGCCAGCA CAATGCCACC CCCTGCTTAT
    AGTGAGATAC AGAGGAGCAA TTTTGTACTG TGCAACAGTC CTGACAAAGT GCCTGTTTCA
    ACTCAAGAGG CATCGCATTC TTACACAATC AGCGATGTAC AAGGCAGAGA GGAGGATTAT
    GAGACATCAG GTGTCGCAAC CAGAAGTGCC TCACAGGACA TAAGGGAAGA AGAGTCAACC
    CCAGATACAG AGGACTATTT CAGTAAAGAC TCTACTCCCA GTGATAATTC ATCCTCTCCT
    TTGACCGATG ACTCCAGACT TGATGATGAT GTTTTCCCAT CTCCCAATAA ACTCCGCACA
    ACTGAAGATC TTTTTGCTAT GATACACAGA TCTAAAAGGA AAGTGCTGGG ACGCAAAGAT
    TCAGGGGAAG TCAGTGGAAA AGGTCGGCCT GTTGTACCAC CTGTGACCAC CCCTGTAAGC
    AATCCTACTG TATGCCCGGT CATCCCTGCT CCCCCAGTTC CTTCAAACAA CTCCCAGCGA
    GCCTCAGGAC CCATCTACAG GAGTGCCAAA AAGTCTAGTA CATCCAGTGA GGAGTTCAAA
    CTCCTTCTGC TTAAGAAGGG TAGTCGCACA GACTCAAGTT ATCGCATGTC TGCTACAGAG
    ATCCTTAAGA GCCCCATCAC ACCCAAGACT CAAGCAGAGC TGCTGTTAGA GGCCATGAGG
    CAACCAGAGG AGATCCCCTT ACCCCAACTG GATTCCACCA GCAGTGGAGA TCCACTTCCA
    AGTCCATTCC CTAAGGCCAA CAGTGAGGGA TTCTCCCCAA AAACACTCAC CATGTCAGCT
    GCCTCCAGAC AAGGACGTTC TAGAATTCCA CCTGCAGCGA ACAGCAGTCG CTATAGCACG
    CGGAGTCGGC TGTACACTGC CCCAATGCAG GCCATATCAG AAGGAGAGAC TGAGAACTCG
    GATGGAAGTC CACACGATGA CCGCTCCTCC TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_021335532.1
    CDS321..5213
    Translation

    Target ORF information:

    RefSeq Version XM_021479857.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio Nance-Horan syndrome b (congenital cataracts and dental anomalies) (nhsb), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_021479857.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    ATGCCTTTTG CCAAACGGAT CGTTGAGCCC CAGCTGCTGG TTAGACATCC CATCCCTAAT 
    GATGAAGGGT TACTGTTCGA GGATCTGTGC TCCATCACCA ATGTGGCTCT ATCCCGGACC
    CTGCGGCAGC TCTCCGACCT GTCCAAACAC GCTTGCTCCT TATTTCAGGA GCTTGAGAAC
    GAAATAGTGT CCACAAACCA ACGGGTTTGG GCTTTGCAGA ACAAAATAGG CAAAATTCAG
    CAAACCGCCA GTGCGCTGGA TCCAAAAATG GAGGCTGTGC GTTTCAGGAG CATCTCTATA
    GCGGTCTCGA ATCTGGACAT TGAGAGTAAA CTGACGTCGC ACTATCAGGC TCCATGGCAT
    CAGCAACACA ATCTGTTCCA CACTTGCACA CGGCCAGCGT GTCTGGAGGA GCTGCACCGA
    CATGCCAAGC TCAACCTGCG GGCGCTGCAC AGAGACCAGC AGCAGCGTCA GCGTTCAACC
    AGCAGAGAGC GCAGTCGGGT CACTATATCA ATATCTGTGG CACCGCCAAT GCCAACCTTC
    CCTTCAACTC ACAAACTCAG ACGGCGAGAG CAGAGAGGTC GACATTCACG CATGGAGAGG
    AGCGTTAGAG AGAGTGATGT GCAAACTATC CAGAGGAAGG AGAGACCAGC CAAGGATTCT
    GAATTCAAAC CTGTCCTCAG AAAGTATCAG CGCTCCCGCT CCCCCTCCCC TGTTCAGTGT
    TGTTACTTCA TTCCCTGGAT TAGAAAGGCT GGAACCAACA CAGAAACGGA TGGAGAACTA
    CAAGTGATGA GCCATAGGCC TAAATGTCCT GTTCCCAATG CACCTACCAC CCTGGACAAA
    CAGACTAACT GGTCTAAAGC CCTGCCTCTT CCCACTCCAG AAGAAAGAAT AAAGAATGAC
    TCTCAAGTGA TTTCATCCTG TATCATTCCA ATAAATGTTT CGGGTGTTGG ATTTGACAGA
    GAAGCCAGTG CTCGCTGCTC TCTTGTTCAC TCGCAATCGG TTCTACAGAG ACGGCGGAAA
    CTCAGGAGGC GAAAAACGAT CACTGGGATC CCCAAACGAG TTCAACAAGA CATGGATTCT
    GACGAATCCC CTGTTGCTAG AGAAAGAACA GTGATAATCC ACGCCAATCC ACACAAGTCC
    CACGAGTGGC ATGAGGAGCT CTCCTTGAGT GGCAGAGTAT TGCATACTAA GGATTCGGGC
    TGTCAGACAG ATGACTTCTT GATTACTGCT CCATCCCGAA GACGCATCCG TGCCCAAAGA
    GGTCAGGGAA TTCCAGCGTC TCTCTCTCAC TCCACTGGAA ACATTACCTC CCTCCCGGAT
    CGTTCTGATA CGGTTTATGC TGCAGCATCA GCCACTCGTG TCCGTTCTAG GAGCCTTCCA
    CGTGAGGGTG GTCGGCTTCT GGATGAGGAC CAAGATGACA GTGATGATGA TGATGATGAA
    GATGATGATG AAGATGATGA AGAGGAAGAT GAGGAGCTCT CTCCATATGA AGCCGAAGAC
    TTCCTGCCAG GTCCCGGACA GAGAATTCCA AAGGATGAGG AAGAGAGTAC TGACGACCAA
    GCCATGCCAG AGCTACAGTT TGGAAGTCTT AAGCGTATGC AGCATTCAGA GAGCCCTGAC
    CACACATGGA TTGAGAGAGG CAGATCCAGA CTCCCACGGA AAGTAGACAT GGGGAGCTGT
    GAGATTTCCT CTAGTTCAGA TACTTTCAGC AGCCCCATAC ACTCTGCATC CACTACAGGA
    GTGCTTGGCA GCCAGATTGA CCATAAAGAG GATCACCAGT CCTCAAGTGG CAACTGGAGT
    GGAAGCAGCT CCACTTGCCC ATCGCAGACA TCCGAGACTC TTCCACCTGC TGCCTCCCCT
    CCCTTGACGG GATCCTCACA CTGTGATTCA GAACTTTCCC TGAATACTGG GTCCCATGTC
    ATAGATGACA ACACTGGTTT CATAATTGAT CCCTATCACA TAGACAAGCC ACAGGGACAG
    GGGCAACGAT CCAATTCATT TACCTCATCA GCTACTGATC AGATGGATGA TGCAGGGGTC
    AGCACTGCTA GTGATGGGGA GTGGAACTGT GCCCAGGACC AGCAGGATCA GTCCTGCACC
    CAAGACTTCA GCCCAAAGCG TTCCAGAGAG GATGAGAGTG GTGTCAGCTG CCCTAGTTAT
    TCTAGTGGGG AAACTTACGA GAGCTTCAGT GACCAAGCAT CCGCTAGAAA ATCTGAGATG
    CACTACATTG TCGACACTGA AGGATTCTAT TCCTCCATGC ACTTTGACTC TGGTCTTAAG
    GGTAGCAGAA GCTACTACAA CTATGCAGCC ATTGACCCCG ACTGTGGCCC AAGTGGTAAT
    ATAGCACCTG GCATGACTGA ATACCCTCAG CCAGAGTACA GAGCCACCAG GACACTGGGC
    AGACCCTGTC TCTCCTTAAG AAAACCAAAG GCCAAGCCAC CCCCACCAAA ACGTAGCTCT
    TCATTAAAGG AAACAAGCAG TGATGTGAAC GTTCCAGAGC AGAACGAACC AAAGATTACT
    TGTGAGTTGC CACTGCCCTT GTCTTCCAGG GAGATGAAAC TGCAGCTGGA CTTGGCTGAT
    TCTGCAGGGC ACCTTGAGAC TGCAGGTCTT GAATCACTTG GTGCATGGGG AGTGGAAAAT
    GTTAGGGACA TGCTTGACTG CTCCTCCCCA TTCAGTTCCT CAGACACACA TTCCTTCAAA
    GATGAAGGTG CTGTGCAAGC AGACTATGCA GACCTCTGGC TTCACAATGA CTTGAAATCA
    AATGATCCTT ATCGGTCCCT CTCTAACTCT AGCTCTGCTA CAGGTACAAC TGTTATTGAA
    TGCATCAAGT CACCAGAAAG ATCAGAAACG CACACCTGTC AACCCAGGTC CAGACCTTCT
    TCCCCAACTC TTCCTCCACC TGAAAGTGAA TTTAAGCTTG CTTCTCCCGA AAAGCTGGCA
    GGCTTGGCGT CCCCTTCCAG TGGATATTCC AGTCAGTCTG AAACACCTAC ATCTTCCTTC
    CCCTCTGCTT TCTTCCCAGG GCCCCTGTCT CCAACCAGTG GGAAGAGGAA GCCCAAAGTC
    CCTGAAAGGA AGTCCTCACT TTGTTCCTTA CAGCAGCAAC AGCTTTCAGT CAGAGACCCA
    GGCATTTCCT GTAGGAGAGA AACTGACTTC TATGCCATAC CCCCAAGTCA CCTTGACCTA
    AGTGCTCTTC ACAGTAAGCA CTTTCCTCAC AGAAATCAAA TGCACATCCT CCACCAGAAT
    AAGCAGAAAG CTGCCATAGC AGCAGCTGCT GCAGCAGAAG CTCGCAGTGC AGCAGCTGCT
    GCAGCAGAAG CTCGCAGTGC AGCAGCTGCT GCAGCAGAAG CTCGCAGTGC AGCAACTGCA
    GCCGCTGCTG CCGAGGCCTG CACTGCAGCT ACAGCTTCTA CTAAAGAGAG TGCAACAAAT
    ACAGCACCCA TCTCAGCCCA TTTGGCTATT ACTCCAACGG TTCTTAGATC AGTGCAACTG
    CGATCTATAT GTAGACCATC TGATGGCAAC CAAGGGCTTG ATCAAGACAG TTCAAATCTC
    ATAACTCGTC CTAAGTGTCC CACAATAATA ACTGATGCCC CATCATCTAG CAACAGGCAC
    AACAGGAAGC CACCAGCCTA CAAACCCCCT GCTGCACAGG TTTGTGATTC ACAGATTCCA
    CTGGTGGAAA ACATTGTTTT TCCACAAGAA GAAGTGAGAG TGAGACAGGA GAGGTTTGGT
    CCTGGTACTT ACTGGGCAAT GACTGCTTTC AGAACTCCTG TGGACCCTAA TTGTGAAAAA
    GAAGTCTCTT TAAAAAGGTC ATCTCAGTGT TTTCAGCAAG AACAAGACAG CAGAAGGTGC
    CTAGATGTGC AAAAGACATT GTCACCAGAG AGACTAAAAC AAGATTTGAA TCAACTATCG
    CGGCCAGACC CTTCAACACA GTCCATGTGT TTGGCCAGCA CAATGCCACC CCCTGCTTAT
    AGTGAGATAC AGAGGAGCAA TTTTGTACTG TGCAACAGTC CTGACAAAGT GCCTGTTTCA
    ACTCAAGAGG CATCGCATTC TTACACAATC AGCGATGTAC AAGGCAGAGA GGAGGATTAT
    GAGACATCAG GTGTCGCAAC CAGAAGTGCC TCACAGGACA TAAGGGAAGA AGAGTCAACC
    CCAGATACAG AGGACTATTT CAGTAAAGAC TCTACTCCCA GTGATAATTC ATCCTCTCCT
    TTGACCGATG ACTCCAGACT TGATGATGAT GTTTTCCCAT CTCCCAATAA ACTCCGCACA
    ACTGAAGATC TTTTTGCTAT GATACACAGA TCTAAAAGGA AAGTGCTGGG ACGCAAAGAT
    TCAGGGGAAG TCAGTGGAAA AGGTCGGCCT GTTGTACCAC CTGTGACCAC CCCTGTAAGC
    AATCCTACTG TATGCCCGGT CATCCCTGCT CCCCCAGTTC CTTCAAACAA CTCCCAGCGA
    GCCTCAGGAC CCATCTACAG GAGTGCCAAA AAGTCTAGTA CATCCAGTGA GGAGTTCAAA
    CTCCTTCTGC TTAAGAAGGG TAGTCGCACA GACTCAAGTT ATCGCATGTC TGCTACAGAG
    ATCCTTAAGA GCCCCATCAC ACCCAAGACT CAAGCAGAGC TGCTGTTAGA GGCCATGAGG
    CAACCAGAGG AGATCCCCTT ACCCCAACTG GATTCCACCA GCAGTGGAGA TCCACTTCCA
    AGTCCATTCC CTAAGGCCAA CAGTGAGGGA TTCTCCCCAA AAACACTCAC CATGTCAGCT
    GCCTCCAGAC AAGGACGTTC TAGAATTCCA CCTGCAGCGA ACAGCAGTCG CTATAGCACG
    CGGAGTCGGC TGTACACTGC CCCAATGCAG GCCATATCAG AAGGAGAGAC TGAGAACTCG
    GATGGAAGTC CACACGATGA CCGCTCCTCC TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa148025
    Clone ID Related Accession (Same CDS sequence) XM_021479858.1
    Accession Version XM_021479858.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4848bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product Nance-Horan syndrome protein isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007122.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    ATGCCTTTTG CCAAACGGAT CGTTGAGCCC CAGCTGCTGG TTAGACATCC CATCCCTAAT 
    GATGAAGGGT TACTGTTCGA GGATCTGTGC TCCATCACCA ATGTGGCTCT ATCCCGGACC
    CTGCGGCAGC TCTCCGACCT GTCCAAACAC GCTTGCTCCT TATTTCAGGA GCTTGAGAAC
    GAAATAGTGT CCACAAACCA ACGGGTTTGG GCTTTGCAGA ACAAAATAGG CAAAATTCAG
    CAAACCGCCA GTGCGCTGGA TCCAAAAATG GAGGCTGTGC GTTTCAGGAG CATCTCTATA
    GCGGTCTCGA ATCTGGACAT TGAGAGTAAA CTGACGTCGC ACTATCAGGC TCCATGGCAT
    CAGCAACACA ATCTGTTCCA CACTTGCACA CGGCCAGCGT GTCTGGAGGA GCTGCACCGA
    CATGCCAAGC TCAACCTGCG GGCGCTGCAC AGAGACCAGC AGCAGCGTCA GCGTTCAACC
    AGCAGAGAGC GCAGTCGGGT CACTATATCA ATATCTGTGG CACCGCCAAT GCCAACCTTC
    CCTTCAACTC ACAAACTCAG ACGGCGAGAG CAGAGAGGTC GACATTCACG CATGGAGAGA
    CCAGCCAAGG ATTCTGAATT CAAACCTGTC CTCAGAAAGT ATCAGCGCTC CCGCTCCCCC
    TCCCCTGTTC AGTGTTGTTA CTTCATTCCC TGGATTAGAA AGGCTGGAAC CAACACAGAA
    ACGGATGGAG AACTACAAGT GATGAGCCAT AGGCCTAAAT GTCCTGTTCC CAATGCACCT
    ACCACCCTGG ACAAACAGAC TAACTGGTCT AAAGCCCTGC CTCTTCCCAC TCCAGAAGAA
    AGAATAAAGA ATGACTCTCA AGTGATTTCA TCCTGTATCA TTCCAATAAA TGTTTCGGGT
    GTTGGATTTG ACAGAGAAGC CAGTGCTCGC TGCTCTCTTG TTCACTCGCA ATCGGTTCTA
    CAGAGACGGC GGAAACTCAG GAGGCGAAAA ACGATCACTG GGATCCCCAA ACGAGTTCAA
    CAAGACATGG ATTCTGACGA ATCCCCTGTT GCTAGAGAAA GAACAGTGAT AATCCACGCC
    AATCCACACA AGTCCCACGA GTGGCATGAG GAGCTCTCCT TGAGTGGCAG AGTATTGCAT
    ACTAAGGATT CGGGCTGTCA GACAGATGAC TTCTTGATTA CTGCTCCATC CCGAAGACGC
    ATCCGTGCCC AAAGAGGTCA GGGAATTCCA GCGTCTCTCT CTCACTCCAC TGGAAACATT
    ACCTCCCTCC CGGATCGTTC TGATACGGTT TATGCTGCAG CATCAGCCAC TCGTGTCCGT
    TCTAGGAGCC TTCCACGTGA GGGTGGTCGG CTTCTGGATG AGGACCAAGA TGACAGTGAT
    GATGATGATG ATGAAGATGA TGATGAAGAT GATGAAGAGG AAGATGAGGA GCTCTCTCCA
    TATGAAGCCG AAGACTTCCT GCCAGGTCCC GGACAGAGAA TTCCAAAGGA TGAGGAAGAG
    AGTACTGACG ACCAAGCCAT GCCAGAGCTA CAGTTTGGAA GTCTTAAGCG TATGCAGCAT
    TCAGAGAGCC CTGACCACAC ATGGATTGAG AGAGGCAGAT CCAGACTCCC ACGGAAAGTA
    GACATGGGGA GCTGTGAGAT TTCCTCTAGT TCAGATACTT TCAGCAGCCC CATACACTCT
    GCATCCACTA CAGGAGTGCT TGGCAGCCAG ATTGACCATA AAGAGGATCA CCAGTCCTCA
    AGTGGCAACT GGAGTGGAAG CAGCTCCACT TGCCCATCGC AGACATCCGA GACTCTTCCA
    CCTGCTGCCT CCCCTCCCTT GACGGGATCC TCACACTGTG ATTCAGAACT TTCCCTGAAT
    ACTGGGTCCC ATGTCATAGA TGACAACACT GGTTTCATAA TTGATCCCTA TCACATAGAC
    AAGCCACAGG GACAGGGGCA ACGATCCAAT TCATTTACCT CATCAGCTAC TGATCAGATG
    GATGATGCAG GGGTCAGCAC TGCTAGTGAT GGGGAGTGGA ACTGTGCCCA GGACCAGCAG
    GATCAGTCCT GCACCCAAGA CTTCAGCCCA AAGCGTTCCA GAGAGGATGA GAGTGGTGTC
    AGCTGCCCTA GTTATTCTAG TGGGGAAACT TACGAGAGCT TCAGTGACCA AGCATCCGCT
    AGAAAATCTG AGATGCACTA CATTGTCGAC ACTGAAGGAT TCTATTCCTC CATGCACTTT
    GACTCTGGTC TTAAGGGTAG CAGAAGCTAC TACAACTATG CAGCCATTGA CCCCGACTGT
    GGCCCAAGTG GTAATATAGC ACCTGGCATG ACTGAATACC CTCAGCCAGA GTACAGAGCC
    ACCAGGACAC TGGGCAGACC CTGTCTCTCC TTAAGAAAAC CAAAGGCCAA GCCACCCCCA
    CCAAAACGTA GCTCTTCATT AAAGGAAACA AGCAGTGATG TGAACGTTCC AGAGCAGAAC
    GAACCAAAGA TTACTTGTGA GTTGCCACTG CCCTTGTCTT CCAGGGAGAT GAAACTGCAG
    CTGGACTTGG CTGATTCTGC AGGGCACCTT GAGACTGCAG GTCTTGAATC ACTTGGTGCA
    TGGGGAGTGG AAAATGTTAG GGACATGCTT GACTGCTCCT CCCCATTCAG TTCCTCAGAC
    ACACATTCCT TCAAAGATGA AGGTGCTGTG CAAGCAGACT ATGCAGACCT CTGGCTTCAC
    AATGACTTGA AATCAAATGA TCCTTATCGG TCCCTCTCTA ACTCTAGCTC TGCTACAGGT
    ACAACTGTTA TTGAATGCAT CAAGTCACCA GAAAGATCAG AAACGCACAC CTGTCAACCC
    AGGTCCAGAC CTTCTTCCCC AACTCTTCCT CCACCTGAAA GTGAATTTAA GCTTGCTTCT
    CCCGAAAAGC TGGCAGGCTT GGCGTCCCCT TCCAGTGGAT ATTCCAGTCA GTCTGAAACA
    CCTACATCTT CCTTCCCCTC TGCTTTCTTC CCAGGGCCCC TGTCTCCAAC CAGTGGGAAG
    AGGAAGCCCA AAGTCCCTGA AAGGAAGTCC TCACTTTGTT CCTTACAGCA GCAACAGCTT
    TCAGTCAGAG ACCCAGGCAT TTCCTGTAGG AGAGAAACTG ACTTCTATGC CATACCCCCA
    AGTCACCTTG ACCTAAGTGC TCTTCACAGT AAGCACTTTC CTCACAGAAA TCAAATGCAC
    ATCCTCCACC AGAATAAGCA GAAAGCTGCC ATAGCAGCAG CTGCTGCAGC AGAAGCTCGC
    AGTGCAGCAG CTGCTGCAGC AGAAGCTCGC AGTGCAGCAG CTGCTGCAGC AGAAGCTCGC
    AGTGCAGCAA CTGCAGCCGC TGCTGCCGAG GCCTGCACTG CAGCTACAGC TTCTACTAAA
    GAGAGTGCAA CAAATACAGC ACCCATCTCA GCCCATTTGG CTATTACTCC AACGGTTCTT
    AGATCAGTGC AACTGCGATC TATATGTAGA CCATCTGATG GCAACCAAGG GCTTGATCAA
    GACAGTTCAA ATCTCATAAC TCGTCCTAAG TGTCCCACAA TAATAACTGA TGCCCCATCA
    TCTAGCAACA GGCACAACAG GAAGCCACCA GCCTACAAAC CCCCTGCTGC ACAGGTTTGT
    GATTCACAGA TTCCACTGGT GGAAAACATT GTTTTTCCAC AAGAAGAAGT GAGAGTGAGA
    CAGGAGAGGT TTGGTCCTGG TACTTACTGG GCAATGACTG CTTTCAGAAC TCCTGTGGAC
    CCTAATTGTG AAAAAGAAGT CTCTTTAAAA AGGTCATCTC AGTGTTTTCA GCAAGAACAA
    GACAGCAGAA GGTGCCTAGA TGTGCAAAAG ACATTGTCAC CAGAGAGACT AAAACAAGAT
    TTGAATCAAC TATCGCGGCC AGACCCTTCA ACACAGTCCA TGTGTTTGGC CAGCACAATG
    CCACCCCCTG CTTATAGTGA GATACAGAGG AGCAATTTTG TACTGTGCAA CAGTCCTGAC
    AAAGTGCCTG TTTCAACTCA AGAGGCATCG CATTCTTACA CAATCAGCGA TGTACAAGGC
    AGAGAGGAGG ATTATGAGAC ATCAGGTGTC GCAACCAGAA GTGCCTCACA GGACATAAGG
    GAAGAAGAGT CAACCCCAGA TACAGAGGAC TATTTCAGTA AAGACTCTAC TCCCAGTGAT
    AATTCATCCT CTCCTTTGAC CGATGACTCC AGACTTGATG ATGATGTTTT CCCATCTCCC
    AATAAACTCC GCACAACTGA AGATCTTTTT GCTATGATAC ACAGATCTAA AAGGAAAGTG
    CTGGGACGCA AAGATTCAGG GGAAGTCAGT GGAAAAGGTC GGCCTGTTGT ACCACCTGTG
    ACCACCCCTG TAAGCAATCC TACTGTATGC CCGGTCATCC CTGCTCCCCC AGTTCCTTCA
    AACAACTCCC AGCGAGCCTC AGGACCCATC TACAGGAGTG CCAAAAAGTC TAGTACATCC
    AGTGAGGAGT TCAAACTCCT TCTGCTTAAG AAGGGTAGTC GCACAGACTC AAGTTATCGC
    ATGTCTGCTA CAGAGATCCT TAAGAGCCCC ATCACACCCA AGACTCAAGC AGAGCTGCTG
    TTAGAGGCCA TGAGGCAACC AGAGGAGATC CCCTTACCCC AACTGGATTC CACCAGCAGT
    GGAGATCCAC TTCCAAGTCC ATTCCCTAAG GCCAACAGTG AGGGATTCTC CCCAAAAACA
    CTCACCATGT CAGCTGCCTC CAGACAAGGA CGTTCTAGAA TTCCACCTGC AGCGAACAGC
    AGTCGCTATA GCACGCGGAG TCGGCTGTAC ACTGCCCCAA TGCAGGCCAT ATCAGAAGGA
    GAGACTGAGA ACTCGGATGG AAGTCCACAC GATGACCGCT CCTCCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_021335533.1
    CDS321..5168
    Translation

    Target ORF information:

    RefSeq Version XM_021479858.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio Nance-Horan syndrome b (congenital cataracts and dental anomalies) (nhsb), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_021479858.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    ATGCCTTTTG CCAAACGGAT CGTTGAGCCC CAGCTGCTGG TTAGACATCC CATCCCTAAT 
    GATGAAGGGT TACTGTTCGA GGATCTGTGC TCCATCACCA ATGTGGCTCT ATCCCGGACC
    CTGCGGCAGC TCTCCGACCT GTCCAAACAC GCTTGCTCCT TATTTCAGGA GCTTGAGAAC
    GAAATAGTGT CCACAAACCA ACGGGTTTGG GCTTTGCAGA ACAAAATAGG CAAAATTCAG
    CAAACCGCCA GTGCGCTGGA TCCAAAAATG GAGGCTGTGC GTTTCAGGAG CATCTCTATA
    GCGGTCTCGA ATCTGGACAT TGAGAGTAAA CTGACGTCGC ACTATCAGGC TCCATGGCAT
    CAGCAACACA ATCTGTTCCA CACTTGCACA CGGCCAGCGT GTCTGGAGGA GCTGCACCGA
    CATGCCAAGC TCAACCTGCG GGCGCTGCAC AGAGACCAGC AGCAGCGTCA GCGTTCAACC
    AGCAGAGAGC GCAGTCGGGT CACTATATCA ATATCTGTGG CACCGCCAAT GCCAACCTTC
    CCTTCAACTC ACAAACTCAG ACGGCGAGAG CAGAGAGGTC GACATTCACG CATGGAGAGA
    CCAGCCAAGG ATTCTGAATT CAAACCTGTC CTCAGAAAGT ATCAGCGCTC CCGCTCCCCC
    TCCCCTGTTC AGTGTTGTTA CTTCATTCCC TGGATTAGAA AGGCTGGAAC CAACACAGAA
    ACGGATGGAG AACTACAAGT GATGAGCCAT AGGCCTAAAT GTCCTGTTCC CAATGCACCT
    ACCACCCTGG ACAAACAGAC TAACTGGTCT AAAGCCCTGC CTCTTCCCAC TCCAGAAGAA
    AGAATAAAGA ATGACTCTCA AGTGATTTCA TCCTGTATCA TTCCAATAAA TGTTTCGGGT
    GTTGGATTTG ACAGAGAAGC CAGTGCTCGC TGCTCTCTTG TTCACTCGCA ATCGGTTCTA
    CAGAGACGGC GGAAACTCAG GAGGCGAAAA ACGATCACTG GGATCCCCAA ACGAGTTCAA
    CAAGACATGG ATTCTGACGA ATCCCCTGTT GCTAGAGAAA GAACAGTGAT AATCCACGCC
    AATCCACACA AGTCCCACGA GTGGCATGAG GAGCTCTCCT TGAGTGGCAG AGTATTGCAT
    ACTAAGGATT CGGGCTGTCA GACAGATGAC TTCTTGATTA CTGCTCCATC CCGAAGACGC
    ATCCGTGCCC AAAGAGGTCA GGGAATTCCA GCGTCTCTCT CTCACTCCAC TGGAAACATT
    ACCTCCCTCC CGGATCGTTC TGATACGGTT TATGCTGCAG CATCAGCCAC TCGTGTCCGT
    TCTAGGAGCC TTCCACGTGA GGGTGGTCGG CTTCTGGATG AGGACCAAGA TGACAGTGAT
    GATGATGATG ATGAAGATGA TGATGAAGAT GATGAAGAGG AAGATGAGGA GCTCTCTCCA
    TATGAAGCCG AAGACTTCCT GCCAGGTCCC GGACAGAGAA TTCCAAAGGA TGAGGAAGAG
    AGTACTGACG ACCAAGCCAT GCCAGAGCTA CAGTTTGGAA GTCTTAAGCG TATGCAGCAT
    TCAGAGAGCC CTGACCACAC ATGGATTGAG AGAGGCAGAT CCAGACTCCC ACGGAAAGTA
    GACATGGGGA GCTGTGAGAT TTCCTCTAGT TCAGATACTT TCAGCAGCCC CATACACTCT
    GCATCCACTA CAGGAGTGCT TGGCAGCCAG ATTGACCATA AAGAGGATCA CCAGTCCTCA
    AGTGGCAACT GGAGTGGAAG CAGCTCCACT TGCCCATCGC AGACATCCGA GACTCTTCCA
    CCTGCTGCCT CCCCTCCCTT GACGGGATCC TCACACTGTG ATTCAGAACT TTCCCTGAAT
    ACTGGGTCCC ATGTCATAGA TGACAACACT GGTTTCATAA TTGATCCCTA TCACATAGAC
    AAGCCACAGG GACAGGGGCA ACGATCCAAT TCATTTACCT CATCAGCTAC TGATCAGATG
    GATGATGCAG GGGTCAGCAC TGCTAGTGAT GGGGAGTGGA ACTGTGCCCA GGACCAGCAG
    GATCAGTCCT GCACCCAAGA CTTCAGCCCA AAGCGTTCCA GAGAGGATGA GAGTGGTGTC
    AGCTGCCCTA GTTATTCTAG TGGGGAAACT TACGAGAGCT TCAGTGACCA AGCATCCGCT
    AGAAAATCTG AGATGCACTA CATTGTCGAC ACTGAAGGAT TCTATTCCTC CATGCACTTT
    GACTCTGGTC TTAAGGGTAG CAGAAGCTAC TACAACTATG CAGCCATTGA CCCCGACTGT
    GGCCCAAGTG GTAATATAGC ACCTGGCATG ACTGAATACC CTCAGCCAGA GTACAGAGCC
    ACCAGGACAC TGGGCAGACC CTGTCTCTCC TTAAGAAAAC CAAAGGCCAA GCCACCCCCA
    CCAAAACGTA GCTCTTCATT AAAGGAAACA AGCAGTGATG TGAACGTTCC AGAGCAGAAC
    GAACCAAAGA TTACTTGTGA GTTGCCACTG CCCTTGTCTT CCAGGGAGAT GAAACTGCAG
    CTGGACTTGG CTGATTCTGC AGGGCACCTT GAGACTGCAG GTCTTGAATC ACTTGGTGCA
    TGGGGAGTGG AAAATGTTAG GGACATGCTT GACTGCTCCT CCCCATTCAG TTCCTCAGAC
    ACACATTCCT TCAAAGATGA AGGTGCTGTG CAAGCAGACT ATGCAGACCT CTGGCTTCAC
    AATGACTTGA AATCAAATGA TCCTTATCGG TCCCTCTCTA ACTCTAGCTC TGCTACAGGT
    ACAACTGTTA TTGAATGCAT CAAGTCACCA GAAAGATCAG AAACGCACAC CTGTCAACCC
    AGGTCCAGAC CTTCTTCCCC AACTCTTCCT CCACCTGAAA GTGAATTTAA GCTTGCTTCT
    CCCGAAAAGC TGGCAGGCTT GGCGTCCCCT TCCAGTGGAT ATTCCAGTCA GTCTGAAACA
    CCTACATCTT CCTTCCCCTC TGCTTTCTTC CCAGGGCCCC TGTCTCCAAC CAGTGGGAAG
    AGGAAGCCCA AAGTCCCTGA AAGGAAGTCC TCACTTTGTT CCTTACAGCA GCAACAGCTT
    TCAGTCAGAG ACCCAGGCAT TTCCTGTAGG AGAGAAACTG ACTTCTATGC CATACCCCCA
    AGTCACCTTG ACCTAAGTGC TCTTCACAGT AAGCACTTTC CTCACAGAAA TCAAATGCAC
    ATCCTCCACC AGAATAAGCA GAAAGCTGCC ATAGCAGCAG CTGCTGCAGC AGAAGCTCGC
    AGTGCAGCAG CTGCTGCAGC AGAAGCTCGC AGTGCAGCAG CTGCTGCAGC AGAAGCTCGC
    AGTGCAGCAA CTGCAGCCGC TGCTGCCGAG GCCTGCACTG CAGCTACAGC TTCTACTAAA
    GAGAGTGCAA CAAATACAGC ACCCATCTCA GCCCATTTGG CTATTACTCC AACGGTTCTT
    AGATCAGTGC AACTGCGATC TATATGTAGA CCATCTGATG GCAACCAAGG GCTTGATCAA
    GACAGTTCAA ATCTCATAAC TCGTCCTAAG TGTCCCACAA TAATAACTGA TGCCCCATCA
    TCTAGCAACA GGCACAACAG GAAGCCACCA GCCTACAAAC CCCCTGCTGC ACAGGTTTGT
    GATTCACAGA TTCCACTGGT GGAAAACATT GTTTTTCCAC AAGAAGAAGT GAGAGTGAGA
    CAGGAGAGGT TTGGTCCTGG TACTTACTGG GCAATGACTG CTTTCAGAAC TCCTGTGGAC
    CCTAATTGTG AAAAAGAAGT CTCTTTAAAA AGGTCATCTC AGTGTTTTCA GCAAGAACAA
    GACAGCAGAA GGTGCCTAGA TGTGCAAAAG ACATTGTCAC CAGAGAGACT AAAACAAGAT
    TTGAATCAAC TATCGCGGCC AGACCCTTCA ACACAGTCCA TGTGTTTGGC CAGCACAATG
    CCACCCCCTG CTTATAGTGA GATACAGAGG AGCAATTTTG TACTGTGCAA CAGTCCTGAC
    AAAGTGCCTG TTTCAACTCA AGAGGCATCG CATTCTTACA CAATCAGCGA TGTACAAGGC
    AGAGAGGAGG ATTATGAGAC ATCAGGTGTC GCAACCAGAA GTGCCTCACA GGACATAAGG
    GAAGAAGAGT CAACCCCAGA TACAGAGGAC TATTTCAGTA AAGACTCTAC TCCCAGTGAT
    AATTCATCCT CTCCTTTGAC CGATGACTCC AGACTTGATG ATGATGTTTT CCCATCTCCC
    AATAAACTCC GCACAACTGA AGATCTTTTT GCTATGATAC ACAGATCTAA AAGGAAAGTG
    CTGGGACGCA AAGATTCAGG GGAAGTCAGT GGAAAAGGTC GGCCTGTTGT ACCACCTGTG
    ACCACCCCTG TAAGCAATCC TACTGTATGC CCGGTCATCC CTGCTCCCCC AGTTCCTTCA
    AACAACTCCC AGCGAGCCTC AGGACCCATC TACAGGAGTG CCAAAAAGTC TAGTACATCC
    AGTGAGGAGT TCAAACTCCT TCTGCTTAAG AAGGGTAGTC GCACAGACTC AAGTTATCGC
    ATGTCTGCTA CAGAGATCCT TAAGAGCCCC ATCACACCCA AGACTCAAGC AGAGCTGCTG
    TTAGAGGCCA TGAGGCAACC AGAGGAGATC CCCTTACCCC AACTGGATTC CACCAGCAGT
    GGAGATCCAC TTCCAAGTCC ATTCCCTAAG GCCAACAGTG AGGGATTCTC CCCAAAAACA
    CTCACCATGT CAGCTGCCTC CAGACAAGGA CGTTCTAGAA TTCCACCTGC AGCGAACAGC
    AGTCGCTATA GCACGCGGAG TCGGCTGTAC ACTGCCCCAA TGCAGGCCAT ATCAGAAGGA
    GAGACTGAGA ACTCGGATGG AAGTCCACAC GATGACCGCT CCTCCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa148026
    Clone ID Related Accession (Same CDS sequence) XM_021479859.1
    Accession Version XM_021479859.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4785bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product Nance-Horan syndrome protein isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007122.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    ATGCCTTTTG CCAAACGGAT CGTTGAGCCC CAGCTGCTGG TTAGACATCC CATCCCTAAT 
    GATGAAGGGT TACTGTTCGA GGATCTGTGC TCCATCACCA ATGTGGCTCT ATCCCGGACC
    CTGCGGCAGC TCTCCGACCT GTCCAAACAC GCTTGCTCCT TATTTCAGGA GCTTGAGAAC
    GAAATAGTGT CCACAAACCA ACGGGTTTGG GCTTTGCAGA ACAAAATAGG CAAAATTCAG
    CAAACCGCCA GTGCGCTGGA TCCAAAAATG GAGGCTGTGC GTTTCAGGAG CATCTCTATA
    GCGGTCTCGA ATCTGGACAT TGAGAGTAAA CTGACGTCGC ACTATCAGGC TCCATGGCAT
    CAGCAACACA ATCTGTTCCA CACTTGCACA CGGCCAGCGT GTCTGGAGGA GCTGCACCGA
    CATGCCAAGC TCAACCTGCG GGCGCTGCAC AGAGACCAGC AGCAGCGTCA GCGTTCAACC
    AGCAGAGAGC GCAGTCGGGT CACTATATCA ATATCTGTGG CACCGCCAAT GCCAACCTTC
    CCTTCAACTC ACAAACTCAG ACGGCGAGAG CAGAGAGGTC GACATTCACG CATGGAGAGA
    CCAGCCAAGG ATTCTGAATT CAAACCTGTC CTCAGAAAGG CTGGAACCAA CACAGAAACG
    GATGGAGAAC TACAAGTGAT GAGCCATAGG CCTAAATGTC CTGTTCCCAA TGCACCTACC
    ACCCTGGACA AACAGACTAA CTGGTCTAAA GCCCTGCCTC TTCCCACTCC AGAAGAAAGA
    ATAAAGAATG ACTCTCAAGT GATTTCATCC TGTATCATTC CAATAAATGT TTCGGGTGTT
    GGATTTGACA GAGAAGCCAG TGCTCGCTGC TCTCTTGTTC ACTCGCAATC GGTTCTACAG
    AGACGGCGGA AACTCAGGAG GCGAAAAACG ATCACTGGGA TCCCCAAACG AGTTCAACAA
    GACATGGATT CTGACGAATC CCCTGTTGCT AGAGAAAGAA CAGTGATAAT CCACGCCAAT
    CCACACAAGT CCCACGAGTG GCATGAGGAG CTCTCCTTGA GTGGCAGAGT ATTGCATACT
    AAGGATTCGG GCTGTCAGAC AGATGACTTC TTGATTACTG CTCCATCCCG AAGACGCATC
    CGTGCCCAAA GAGGTCAGGG AATTCCAGCG TCTCTCTCTC ACTCCACTGG AAACATTACC
    TCCCTCCCGG ATCGTTCTGA TACGGTTTAT GCTGCAGCAT CAGCCACTCG TGTCCGTTCT
    AGGAGCCTTC CACGTGAGGG TGGTCGGCTT CTGGATGAGG ACCAAGATGA CAGTGATGAT
    GATGATGATG AAGATGATGA TGAAGATGAT GAAGAGGAAG ATGAGGAGCT CTCTCCATAT
    GAAGCCGAAG ACTTCCTGCC AGGTCCCGGA CAGAGAATTC CAAAGGATGA GGAAGAGAGT
    ACTGACGACC AAGCCATGCC AGAGCTACAG TTTGGAAGTC TTAAGCGTAT GCAGCATTCA
    GAGAGCCCTG ACCACACATG GATTGAGAGA GGCAGATCCA GACTCCCACG GAAAGTAGAC
    ATGGGGAGCT GTGAGATTTC CTCTAGTTCA GATACTTTCA GCAGCCCCAT ACACTCTGCA
    TCCACTACAG GAGTGCTTGG CAGCCAGATT GACCATAAAG AGGATCACCA GTCCTCAAGT
    GGCAACTGGA GTGGAAGCAG CTCCACTTGC CCATCGCAGA CATCCGAGAC TCTTCCACCT
    GCTGCCTCCC CTCCCTTGAC GGGATCCTCA CACTGTGATT CAGAACTTTC CCTGAATACT
    GGGTCCCATG TCATAGATGA CAACACTGGT TTCATAATTG ATCCCTATCA CATAGACAAG
    CCACAGGGAC AGGGGCAACG ATCCAATTCA TTTACCTCAT CAGCTACTGA TCAGATGGAT
    GATGCAGGGG TCAGCACTGC TAGTGATGGG GAGTGGAACT GTGCCCAGGA CCAGCAGGAT
    CAGTCCTGCA CCCAAGACTT CAGCCCAAAG CGTTCCAGAG AGGATGAGAG TGGTGTCAGC
    TGCCCTAGTT ATTCTAGTGG GGAAACTTAC GAGAGCTTCA GTGACCAAGC ATCCGCTAGA
    AAATCTGAGA TGCACTACAT TGTCGACACT GAAGGATTCT ATTCCTCCAT GCACTTTGAC
    TCTGGTCTTA AGGGTAGCAG AAGCTACTAC AACTATGCAG CCATTGACCC CGACTGTGGC
    CCAAGTGGTA ATATAGCACC TGGCATGACT GAATACCCTC AGCCAGAGTA CAGAGCCACC
    AGGACACTGG GCAGACCCTG TCTCTCCTTA AGAAAACCAA AGGCCAAGCC ACCCCCACCA
    AAACGTAGCT CTTCATTAAA GGAAACAAGC AGTGATGTGA ACGTTCCAGA GCAGAACGAA
    CCAAAGATTA CTTGTGAGTT GCCACTGCCC TTGTCTTCCA GGGAGATGAA ACTGCAGCTG
    GACTTGGCTG ATTCTGCAGG GCACCTTGAG ACTGCAGGTC TTGAATCACT TGGTGCATGG
    GGAGTGGAAA ATGTTAGGGA CATGCTTGAC TGCTCCTCCC CATTCAGTTC CTCAGACACA
    CATTCCTTCA AAGATGAAGG TGCTGTGCAA GCAGACTATG CAGACCTCTG GCTTCACAAT
    GACTTGAAAT CAAATGATCC TTATCGGTCC CTCTCTAACT CTAGCTCTGC TACAGGTACA
    ACTGTTATTG AATGCATCAA GTCACCAGAA AGATCAGAAA CGCACACCTG TCAACCCAGG
    TCCAGACCTT CTTCCCCAAC TCTTCCTCCA CCTGAAAGTG AATTTAAGCT TGCTTCTCCC
    GAAAAGCTGG CAGGCTTGGC GTCCCCTTCC AGTGGATATT CCAGTCAGTC TGAAACACCT
    ACATCTTCCT TCCCCTCTGC TTTCTTCCCA GGGCCCCTGT CTCCAACCAG TGGGAAGAGG
    AAGCCCAAAG TCCCTGAAAG GAAGTCCTCA CTTTGTTCCT TACAGCAGCA ACAGCTTTCA
    GTCAGAGACC CAGGCATTTC CTGTAGGAGA GAAACTGACT TCTATGCCAT ACCCCCAAGT
    CACCTTGACC TAAGTGCTCT TCACAGTAAG CACTTTCCTC ACAGAAATCA AATGCACATC
    CTCCACCAGA ATAAGCAGAA AGCTGCCATA GCAGCAGCTG CTGCAGCAGA AGCTCGCAGT
    GCAGCAGCTG CTGCAGCAGA AGCTCGCAGT GCAGCAGCTG CTGCAGCAGA AGCTCGCAGT
    GCAGCAACTG CAGCCGCTGC TGCCGAGGCC TGCACTGCAG CTACAGCTTC TACTAAAGAG
    AGTGCAACAA ATACAGCACC CATCTCAGCC CATTTGGCTA TTACTCCAAC GGTTCTTAGA
    TCAGTGCAAC TGCGATCTAT ATGTAGACCA TCTGATGGCA ACCAAGGGCT TGATCAAGAC
    AGTTCAAATC TCATAACTCG TCCTAAGTGT CCCACAATAA TAACTGATGC CCCATCATCT
    AGCAACAGGC ACAACAGGAA GCCACCAGCC TACAAACCCC CTGCTGCACA GGTTTGTGAT
    TCACAGATTC CACTGGTGGA AAACATTGTT TTTCCACAAG AAGAAGTGAG AGTGAGACAG
    GAGAGGTTTG GTCCTGGTAC TTACTGGGCA ATGACTGCTT TCAGAACTCC TGTGGACCCT
    AATTGTGAAA AAGAAGTCTC TTTAAAAAGG TCATCTCAGT GTTTTCAGCA AGAACAAGAC
    AGCAGAAGGT GCCTAGATGT GCAAAAGACA TTGTCACCAG AGAGACTAAA ACAAGATTTG
    AATCAACTAT CGCGGCCAGA CCCTTCAACA CAGTCCATGT GTTTGGCCAG CACAATGCCA
    CCCCCTGCTT ATAGTGAGAT ACAGAGGAGC AATTTTGTAC TGTGCAACAG TCCTGACAAA
    GTGCCTGTTT CAACTCAAGA GGCATCGCAT TCTTACACAA TCAGCGATGT ACAAGGCAGA
    GAGGAGGATT ATGAGACATC AGGTGTCGCA ACCAGAAGTG CCTCACAGGA CATAAGGGAA
    GAAGAGTCAA CCCCAGATAC AGAGGACTAT TTCAGTAAAG ACTCTACTCC CAGTGATAAT
    TCATCCTCTC CTTTGACCGA TGACTCCAGA CTTGATGATG ATGTTTTCCC ATCTCCCAAT
    AAACTCCGCA CAACTGAAGA TCTTTTTGCT ATGATACACA GATCTAAAAG GAAAGTGCTG
    GGACGCAAAG ATTCAGGGGA AGTCAGTGGA AAAGGTCGGC CTGTTGTACC ACCTGTGACC
    ACCCCTGTAA GCAATCCTAC TGTATGCCCG GTCATCCCTG CTCCCCCAGT TCCTTCAAAC
    AACTCCCAGC GAGCCTCAGG ACCCATCTAC AGGAGTGCCA AAAAGTCTAG TACATCCAGT
    GAGGAGTTCA AACTCCTTCT GCTTAAGAAG GGTAGTCGCA CAGACTCAAG TTATCGCATG
    TCTGCTACAG AGATCCTTAA GAGCCCCATC ACACCCAAGA CTCAAGCAGA GCTGCTGTTA
    GAGGCCATGA GGCAACCAGA GGAGATCCCC TTACCCCAAC TGGATTCCAC CAGCAGTGGA
    GATCCACTTC CAAGTCCATT CCCTAAGGCC AACAGTGAGG GATTCTCCCC AAAAACACTC
    ACCATGTCAG CTGCCTCCAG ACAAGGACGT TCTAGAATTC CACCTGCAGC GAACAGCAGT
    CGCTATAGCA CGCGGAGTCG GCTGTACACT GCCCCAATGC AGGCCATATC AGAAGGAGAG
    ACTGAGAACT CGGATGGAAG TCCACACGAT GACCGCTCCT CCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_021335534.1
    CDS320..5104
    Translation

    Target ORF information:

    RefSeq Version XM_021479859.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio Nance-Horan syndrome b (congenital cataracts and dental anomalies) (nhsb), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_021479859.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    ATGCCTTTTG CCAAACGGAT CGTTGAGCCC CAGCTGCTGG TTAGACATCC CATCCCTAAT 
    GATGAAGGGT TACTGTTCGA GGATCTGTGC TCCATCACCA ATGTGGCTCT ATCCCGGACC
    CTGCGGCAGC TCTCCGACCT GTCCAAACAC GCTTGCTCCT TATTTCAGGA GCTTGAGAAC
    GAAATAGTGT CCACAAACCA ACGGGTTTGG GCTTTGCAGA ACAAAATAGG CAAAATTCAG
    CAAACCGCCA GTGCGCTGGA TCCAAAAATG GAGGCTGTGC GTTTCAGGAG CATCTCTATA
    GCGGTCTCGA ATCTGGACAT TGAGAGTAAA CTGACGTCGC ACTATCAGGC TCCATGGCAT
    CAGCAACACA ATCTGTTCCA CACTTGCACA CGGCCAGCGT GTCTGGAGGA GCTGCACCGA
    CATGCCAAGC TCAACCTGCG GGCGCTGCAC AGAGACCAGC AGCAGCGTCA GCGTTCAACC
    AGCAGAGAGC GCAGTCGGGT CACTATATCA ATATCTGTGG CACCGCCAAT GCCAACCTTC
    CCTTCAACTC ACAAACTCAG ACGGCGAGAG CAGAGAGGTC GACATTCACG CATGGAGAGA
    CCAGCCAAGG ATTCTGAATT CAAACCTGTC CTCAGAAAGG CTGGAACCAA CACAGAAACG
    GATGGAGAAC TACAAGTGAT GAGCCATAGG CCTAAATGTC CTGTTCCCAA TGCACCTACC
    ACCCTGGACA AACAGACTAA CTGGTCTAAA GCCCTGCCTC TTCCCACTCC AGAAGAAAGA
    ATAAAGAATG ACTCTCAAGT GATTTCATCC TGTATCATTC CAATAAATGT TTCGGGTGTT
    GGATTTGACA GAGAAGCCAG TGCTCGCTGC TCTCTTGTTC ACTCGCAATC GGTTCTACAG
    AGACGGCGGA AACTCAGGAG GCGAAAAACG ATCACTGGGA TCCCCAAACG AGTTCAACAA
    GACATGGATT CTGACGAATC CCCTGTTGCT AGAGAAAGAA CAGTGATAAT CCACGCCAAT
    CCACACAAGT CCCACGAGTG GCATGAGGAG CTCTCCTTGA GTGGCAGAGT ATTGCATACT
    AAGGATTCGG GCTGTCAGAC AGATGACTTC TTGATTACTG CTCCATCCCG AAGACGCATC
    CGTGCCCAAA GAGGTCAGGG AATTCCAGCG TCTCTCTCTC ACTCCACTGG AAACATTACC
    TCCCTCCCGG ATCGTTCTGA TACGGTTTAT GCTGCAGCAT CAGCCACTCG TGTCCGTTCT
    AGGAGCCTTC CACGTGAGGG TGGTCGGCTT CTGGATGAGG ACCAAGATGA CAGTGATGAT
    GATGATGATG AAGATGATGA TGAAGATGAT GAAGAGGAAG ATGAGGAGCT CTCTCCATAT
    GAAGCCGAAG ACTTCCTGCC AGGTCCCGGA CAGAGAATTC CAAAGGATGA GGAAGAGAGT
    ACTGACGACC AAGCCATGCC AGAGCTACAG TTTGGAAGTC TTAAGCGTAT GCAGCATTCA
    GAGAGCCCTG ACCACACATG GATTGAGAGA GGCAGATCCA GACTCCCACG GAAAGTAGAC
    ATGGGGAGCT GTGAGATTTC CTCTAGTTCA GATACTTTCA GCAGCCCCAT ACACTCTGCA
    TCCACTACAG GAGTGCTTGG CAGCCAGATT GACCATAAAG AGGATCACCA GTCCTCAAGT
    GGCAACTGGA GTGGAAGCAG CTCCACTTGC CCATCGCAGA CATCCGAGAC TCTTCCACCT
    GCTGCCTCCC CTCCCTTGAC GGGATCCTCA CACTGTGATT CAGAACTTTC CCTGAATACT
    GGGTCCCATG TCATAGATGA CAACACTGGT TTCATAATTG ATCCCTATCA CATAGACAAG
    CCACAGGGAC AGGGGCAACG ATCCAATTCA TTTACCTCAT CAGCTACTGA TCAGATGGAT
    GATGCAGGGG TCAGCACTGC TAGTGATGGG GAGTGGAACT GTGCCCAGGA CCAGCAGGAT
    CAGTCCTGCA CCCAAGACTT CAGCCCAAAG CGTTCCAGAG AGGATGAGAG TGGTGTCAGC
    TGCCCTAGTT ATTCTAGTGG GGAAACTTAC GAGAGCTTCA GTGACCAAGC ATCCGCTAGA
    AAATCTGAGA TGCACTACAT TGTCGACACT GAAGGATTCT ATTCCTCCAT GCACTTTGAC
    TCTGGTCTTA AGGGTAGCAG AAGCTACTAC AACTATGCAG CCATTGACCC CGACTGTGGC
    CCAAGTGGTA ATATAGCACC TGGCATGACT GAATACCCTC AGCCAGAGTA CAGAGCCACC
    AGGACACTGG GCAGACCCTG TCTCTCCTTA AGAAAACCAA AGGCCAAGCC ACCCCCACCA
    AAACGTAGCT CTTCATTAAA GGAAACAAGC AGTGATGTGA ACGTTCCAGA GCAGAACGAA
    CCAAAGATTA CTTGTGAGTT GCCACTGCCC TTGTCTTCCA GGGAGATGAA ACTGCAGCTG
    GACTTGGCTG ATTCTGCAGG GCACCTTGAG ACTGCAGGTC TTGAATCACT TGGTGCATGG
    GGAGTGGAAA ATGTTAGGGA CATGCTTGAC TGCTCCTCCC CATTCAGTTC CTCAGACACA
    CATTCCTTCA AAGATGAAGG TGCTGTGCAA GCAGACTATG CAGACCTCTG GCTTCACAAT
    GACTTGAAAT CAAATGATCC TTATCGGTCC CTCTCTAACT CTAGCTCTGC TACAGGTACA
    ACTGTTATTG AATGCATCAA GTCACCAGAA AGATCAGAAA CGCACACCTG TCAACCCAGG
    TCCAGACCTT CTTCCCCAAC TCTTCCTCCA CCTGAAAGTG AATTTAAGCT TGCTTCTCCC
    GAAAAGCTGG CAGGCTTGGC GTCCCCTTCC AGTGGATATT CCAGTCAGTC TGAAACACCT
    ACATCTTCCT TCCCCTCTGC TTTCTTCCCA GGGCCCCTGT CTCCAACCAG TGGGAAGAGG
    AAGCCCAAAG TCCCTGAAAG GAAGTCCTCA CTTTGTTCCT TACAGCAGCA ACAGCTTTCA
    GTCAGAGACC CAGGCATTTC CTGTAGGAGA GAAACTGACT TCTATGCCAT ACCCCCAAGT
    CACCTTGACC TAAGTGCTCT TCACAGTAAG CACTTTCCTC ACAGAAATCA AATGCACATC
    CTCCACCAGA ATAAGCAGAA AGCTGCCATA GCAGCAGCTG CTGCAGCAGA AGCTCGCAGT
    GCAGCAGCTG CTGCAGCAGA AGCTCGCAGT GCAGCAGCTG CTGCAGCAGA AGCTCGCAGT
    GCAGCAACTG CAGCCGCTGC TGCCGAGGCC TGCACTGCAG CTACAGCTTC TACTAAAGAG
    AGTGCAACAA ATACAGCACC CATCTCAGCC CATTTGGCTA TTACTCCAAC GGTTCTTAGA
    TCAGTGCAAC TGCGATCTAT ATGTAGACCA TCTGATGGCA ACCAAGGGCT TGATCAAGAC
    AGTTCAAATC TCATAACTCG TCCTAAGTGT CCCACAATAA TAACTGATGC CCCATCATCT
    AGCAACAGGC ACAACAGGAA GCCACCAGCC TACAAACCCC CTGCTGCACA GGTTTGTGAT
    TCACAGATTC CACTGGTGGA AAACATTGTT TTTCCACAAG AAGAAGTGAG AGTGAGACAG
    GAGAGGTTTG GTCCTGGTAC TTACTGGGCA ATGACTGCTT TCAGAACTCC TGTGGACCCT
    AATTGTGAAA AAGAAGTCTC TTTAAAAAGG TCATCTCAGT GTTTTCAGCA AGAACAAGAC
    AGCAGAAGGT GCCTAGATGT GCAAAAGACA TTGTCACCAG AGAGACTAAA ACAAGATTTG
    AATCAACTAT CGCGGCCAGA CCCTTCAACA CAGTCCATGT GTTTGGCCAG CACAATGCCA
    CCCCCTGCTT ATAGTGAGAT ACAGAGGAGC AATTTTGTAC TGTGCAACAG TCCTGACAAA
    GTGCCTGTTT CAACTCAAGA GGCATCGCAT TCTTACACAA TCAGCGATGT ACAAGGCAGA
    GAGGAGGATT ATGAGACATC AGGTGTCGCA ACCAGAAGTG CCTCACAGGA CATAAGGGAA
    GAAGAGTCAA CCCCAGATAC AGAGGACTAT TTCAGTAAAG ACTCTACTCC CAGTGATAAT
    TCATCCTCTC CTTTGACCGA TGACTCCAGA CTTGATGATG ATGTTTTCCC ATCTCCCAAT
    AAACTCCGCA CAACTGAAGA TCTTTTTGCT ATGATACACA GATCTAAAAG GAAAGTGCTG
    GGACGCAAAG ATTCAGGGGA AGTCAGTGGA AAAGGTCGGC CTGTTGTACC ACCTGTGACC
    ACCCCTGTAA GCAATCCTAC TGTATGCCCG GTCATCCCTG CTCCCCCAGT TCCTTCAAAC
    AACTCCCAGC GAGCCTCAGG ACCCATCTAC AGGAGTGCCA AAAAGTCTAG TACATCCAGT
    GAGGAGTTCA AACTCCTTCT GCTTAAGAAG GGTAGTCGCA CAGACTCAAG TTATCGCATG
    TCTGCTACAG AGATCCTTAA GAGCCCCATC ACACCCAAGA CTCAAGCAGA GCTGCTGTTA
    GAGGCCATGA GGCAACCAGA GGAGATCCCC TTACCCCAAC TGGATTCCAC CAGCAGTGGA
    GATCCACTTC CAAGTCCATT CCCTAAGGCC AACAGTGAGG GATTCTCCCC AAAAACACTC
    ACCATGTCAG CTGCCTCCAG ACAAGGACGT TCTAGAATTC CACCTGCAGC GAACAGCAGT
    CGCTATAGCA CGCGGAGTCG GCTGTACACT GCCCCAATGC AGGCCATATC AGAAGGAGAG
    ACTGAGAACT CGGATGGAAG TCCACACGAT GACCGCTCCT CCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa148027
    Clone ID Related Accession (Same CDS sequence) XM_021479860.1
    Accession Version XM_021479860.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4629bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product Nance-Horan syndrome protein isoform X7
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007122.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    ATGGCTTTTG CCTGCTGTGT TCCTGTCAGA TCGAGAGCGG TCTCGAATCT GGACATTGAG 
    AGTAAACTGA CGTCGCACTA TCAGGCTCCA TGGCATCAGC AACACAATCT GTTCCACACT
    TGCACACGGC CAGCGTGTCT GGAGGAGCTG CACCGACATG CCAAGCTCAA CCTGCGGGCG
    CTGCACAGAG ACCAGCAGCA GCGTCAGCGT TCAACCAGCA GAGAGCGCAG TCGGGTCACT
    ATATCAATAT CTGTGGCACC GCCAATGCCA ACCTTCCCTT CAACTCACAA ACTCAGACGG
    CGAGAGCAGA GAGGTCGACA TTCACGCATG GAGAGGAGCG TTAGAGAGAG TGATGTGCAA
    ACTATCCAGA GGAAGGAGAG ACCAGCCAAG GATTCTGAAT TCAAACCTGT CCTCAGAAAG
    TATCAGCGCT CCCGCTCCCC CTCCCCTGTT CAGTGTTGTT ACTTCATTCC CTGGATTAGA
    AAGGCTGGAA CCAACACAGA AACGGATGGA GAACTACAAG TGATGAGCCA TAGGCCTAAA
    TGTCCTGTTC CCAATGCACC TACCACCCTG GACAAACAGA CTAACTGGTC TAAAGCCCTG
    CCTCTTCCCA CTCCAGAAGA AAGAATAAAG AATGACTCTC AAGTGATTTC ATCCTGTATC
    ATTCCAATAA ATGTTTCGGG TGTTGGATTT GACAGAGAAG CCAGTGCTCG CTGCTCTCTT
    GTTCACTCGC AATCGGTTCT ACAGAGACGG CGGAAACTCA GGAGGCGAAA AACGATCACT
    GGGATCCCCA AACGAGTTCA ACAAGACATG GATTCTGACG AATCCCCTGT TGCTAGAGAA
    AGAACAGTGA TAATCCACGC CAATCCACAC AAGTCCCACG AGTGGCATGA GGAGCTCTCC
    TTGAGTGGCA GAGTATTGCA TACTAAGGAT TCGGGCTGTC AGACAGATGA CTTCTTGATT
    ACTGCTCCAT CCCGAAGACG CATCCGTGCC CAAAGAGGTC AGGGAATTCC AGCGTCTCTC
    TCTCACTCCA CTGGAAACAT TACCTCCCTC CCGGATCGTT CTGATACGGT TTATGCTGCA
    GCATCAGCCA CTCGTGTCCG TTCTAGGAGC CTTCCACGTG AGGGTGGTCG GCTTCTGGAT
    GAGGACCAAG ATGACAGTGA TGATGATGAT GATGAAGATG ATGATGAAGA TGATGAAGAG
    GAAGATGAGG AGCTCTCTCC ATATGAAGCC GAAGACTTCC TGCCAGGTCC CGGACAGAGA
    ATTCCAAAGG ATGAGGAAGA GAGTACTGAC GACCAAGCCA TGCCAGAGCT ACAGTTTGGA
    AGTCTTAAGC GTATGCAGCA TTCAGAGAGC CCTGACCACA CATGGATTGA GAGAGGCAGA
    TCCAGACTCC CACGGAAAGT AGACATGGGG AGCTGTGAGA TTTCCTCTAG TTCAGATACT
    TTCAGCAGCC CCATACACTC TGCATCCACT ACAGGAGTGC TTGGCAGCCA GATTGACCAT
    AAAGAGGATC ACCAGTCCTC AAGTGGCAAC TGGAGTGGAA GCAGCTCCAC TTGCCCATCG
    CAGACATCCG AGACTCTTCC ACCTGCTGCC TCCCCTCCCT TGACGGGATC CTCACACTGT
    GATTCAGAAC TTTCCCTGAA TACTGGGTCC CATGTCATAG ATGACAACAC TGGTTTCATA
    ATTGATCCCT ATCACATAGA CAAGCCACAG GGACAGGGGC AACGATCCAA TTCATTTACC
    TCATCAGCTA CTGATCAGAT GGATGATGCA GGGGTCAGCA CTGCTAGTGA TGGGGAGTGG
    AACTGTGCCC AGGACCAGCA GGATCAGTCC TGCACCCAAG ACTTCAGCCC AAAGCGTTCC
    AGAGAGGATG AGAGTGGTGT CAGCTGCCCT AGTTATTCTA GTGGGGAAAC TTACGAGAGC
    TTCAGTGACC AAGCATCCGC TAGAAAATCT GAGATGCACT ACATTGTCGA CACTGAAGGA
    TTCTATTCCT CCATGCACTT TGACTCTGGT CTTAAGGGTA GCAGAAGCTA CTACAACTAT
    GCAGCCATTG ACCCCGACTG TGGCCCAAGT GGTAATATAG CACCTGGCAT GACTGAATAC
    CCTCAGCCAG AGTACAGAGC CACCAGGACA CTGGGCAGAC CCTGTCTCTC CTTAAGAAAA
    CCAAAGGCCA AGCCACCCCC ACCAAAACGT AGCTCTTCAT TAAAGGAAAC AAGCAGTGAT
    GTGAACGTTC CAGAGCAGAA CGAACCAAAG ATTACTTGTG AGTTGCCACT GCCCTTGTCT
    TCCAGGGAGA TGAAACTGCA GCTGGACTTG GCTGATTCTG CAGGGCACCT TGAGACTGCA
    GGTCTTGAAT CACTTGGTGC ATGGGGAGTG GAAAATGTTA GGGACATGCT TGACTGCTCC
    TCCCCATTCA GTTCCTCAGA CACACATTCC TTCAAAGATG AAGGTGCTGT GCAAGCAGAC
    TATGCAGACC TCTGGCTTCA CAATGACTTG AAATCAAATG ATCCTTATCG GTCCCTCTCT
    AACTCTAGCT CTGCTACAGG TACAACTGTT ATTGAATGCA TCAAGTCACC AGAAAGATCA
    GAAACGCACA CCTGTCAACC CAGGTCCAGA CCTTCTTCCC CAACTCTTCC TCCACCTGAA
    AGTGAATTTA AGCTTGCTTC TCCCGAAAAG CTGGCAGGCT TGGCGTCCCC TTCCAGTGGA
    TATTCCAGTC AGTCTGAAAC ACCTACATCT TCCTTCCCCT CTGCTTTCTT CCCAGGGCCC
    CTGTCTCCAA CCAGTGGGAA GAGGAAGCCC AAAGTCCCTG AAAGGAAGTC CTCACTTTGT
    TCCTTACAGC AGCAACAGCT TTCAGTCAGA GACCCAGGCA TTTCCTGTAG GAGAGAAACT
    GACTTCTATG CCATACCCCC AAGTCACCTT GACCTAAGTG CTCTTCACAG TAAGCACTTT
    CCTCACAGAA ATCAAATGCA CATCCTCCAC CAGAATAAGC AGAAAGCTGC CATAGCAGCA
    GCTGCTGCAG CAGAAGCTCG CAGTGCAGCA GCTGCTGCAG CAGAAGCTCG CAGTGCAGCA
    GCTGCTGCAG CAGAAGCTCG CAGTGCAGCA ACTGCAGCCG CTGCTGCCGA GGCCTGCACT
    GCAGCTACAG CTTCTACTAA AGAGAGTGCA ACAAATACAG CACCCATCTC AGCCCATTTG
    GCTATTACTC CAACGGTTCT TAGATCAGTG CAACTGCGAT CTATATGTAG ACCATCTGAT
    GGCAACCAAG GGCTTGATCA AGACAGTTCA AATCTCATAA CTCGTCCTAA GTGTCCCACA
    ATAATAACTG ATGCCCCATC ATCTAGCAAC AGGCACAACA GGAAGCCACC AGCCTACAAA
    CCCCCTGCTG CACAGGTTTG TGATTCACAG ATTCCACTGG TGGAAAACAT TGTTTTTCCA
    CAAGAAGAAG TGAGAGTGAG ACAGGAGAGG TTTGGTCCTG GTACTTACTG GGCAATGACT
    GCTTTCAGAA CTCCTGTGGA CCCTAATTGT GAAAAAGAAG TCTCTTTAAA AAGGTCATCT
    CAGTGTTTTC AGCAAGAACA AGACAGCAGA AGGTGCCTAG ATGTGCAAAA GACATTGTCA
    CCAGAGAGAC TAAAACAAGA TTTGAATCAA CTATCGCGGC CAGACCCTTC AACACAGTCC
    ATGTGTTTGG CCAGCACAAT GCCACCCCCT GCTTATAGTG AGATACAGAG GAGCAATTTT
    GTACTGTGCA ACAGTCCTGA CAAAGTGCCT GTTTCAACTC AAGAGGCATC GCATTCTTAC
    ACAATCAGCG ATGTACAAGG CAGAGAGGAG GATTATGAGA CATCAGGTGT CGCAACCAGA
    AGTGCCTCAC AGGACATAAG GGAAGAAGAG TCAACCCCAG ATACAGAGGA CTATTTCAGT
    AAAGACTCTA CTCCCAGTGA TAATTCATCC TCTCCTTTGA CCGATGACTC CAGACTTGAT
    GATGATGTTT TCCCATCTCC CAATAAACTC CGCACAACTG AAGATCTTTT TGCTATGATA
    CACAGATCTA AAAGGAAAGT GCTGGGACGC AAAGATTCAG GGGAAGTCAG TGGAAAAGGT
    CGGCCTGTTG TACCACCTGT GACCACCCCT GTAAGCAATC CTACTGTATG CCCGGTCATC
    CCTGCTCCCC CAGTTCCTTC AAACAACTCC CAGCGAGCCT CAGGACCCAT CTACAGGAGT
    GCCAAAAAGT CTAGTACATC CAGTGAGGAG TTCAAACTCC TTCTGCTTAA GAAGGGTAGT
    CGCACAGACT CAAGTTATCG CATGTCTGCT ACAGAGATCC TTAAGAGCCC CATCACACCC
    AAGACTCAAG CAGAGCTGCT GTTAGAGGCC ATGAGGCAAC CAGAGGAGAT CCCCTTACCC
    CAACTGGATT CCACCAGCAG TGGAGATCCA CTTCCAAGTC CATTCCCTAA GGCCAACAGT
    GAGGGATTCT CCCCAAAAAC ACTCACCATG TCAGCTGCCT CCAGACAAGG ACGTTCTAGA
    ATTCCACCTG CAGCGAACAG CAGTCGCTAT AGCACGCGGA GTCGGCTGTA CACTGCCCCA
    ATGCAGGCCA TATCAGAAGG AGAGACTGAG AACTCGGATG GAAGTCCACA CGATGACCGC
    TCCTCCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_021335535.1
    CDS195..4823
    Translation

    Target ORF information:

    RefSeq Version XM_021479860.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio Nance-Horan syndrome b (congenital cataracts and dental anomalies) (nhsb), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_021479860.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    ATGGCTTTTG CCTGCTGTGT TCCTGTCAGA TCGAGAGCGG TCTCGAATCT GGACATTGAG 
    AGTAAACTGA CGTCGCACTA TCAGGCTCCA TGGCATCAGC AACACAATCT GTTCCACACT
    TGCACACGGC CAGCGTGTCT GGAGGAGCTG CACCGACATG CCAAGCTCAA CCTGCGGGCG
    CTGCACAGAG ACCAGCAGCA GCGTCAGCGT TCAACCAGCA GAGAGCGCAG TCGGGTCACT
    ATATCAATAT CTGTGGCACC GCCAATGCCA ACCTTCCCTT CAACTCACAA ACTCAGACGG
    CGAGAGCAGA GAGGTCGACA TTCACGCATG GAGAGGAGCG TTAGAGAGAG TGATGTGCAA
    ACTATCCAGA GGAAGGAGAG ACCAGCCAAG GATTCTGAAT TCAAACCTGT CCTCAGAAAG
    TATCAGCGCT CCCGCTCCCC CTCCCCTGTT CAGTGTTGTT ACTTCATTCC CTGGATTAGA
    AAGGCTGGAA CCAACACAGA AACGGATGGA GAACTACAAG TGATGAGCCA TAGGCCTAAA
    TGTCCTGTTC CCAATGCACC TACCACCCTG GACAAACAGA CTAACTGGTC TAAAGCCCTG
    CCTCTTCCCA CTCCAGAAGA AAGAATAAAG AATGACTCTC AAGTGATTTC ATCCTGTATC
    ATTCCAATAA ATGTTTCGGG TGTTGGATTT GACAGAGAAG CCAGTGCTCG CTGCTCTCTT
    GTTCACTCGC AATCGGTTCT ACAGAGACGG CGGAAACTCA GGAGGCGAAA AACGATCACT
    GGGATCCCCA AACGAGTTCA ACAAGACATG GATTCTGACG AATCCCCTGT TGCTAGAGAA
    AGAACAGTGA TAATCCACGC CAATCCACAC AAGTCCCACG AGTGGCATGA GGAGCTCTCC
    TTGAGTGGCA GAGTATTGCA TACTAAGGAT TCGGGCTGTC AGACAGATGA CTTCTTGATT
    ACTGCTCCAT CCCGAAGACG CATCCGTGCC CAAAGAGGTC AGGGAATTCC AGCGTCTCTC
    TCTCACTCCA CTGGAAACAT TACCTCCCTC CCGGATCGTT CTGATACGGT TTATGCTGCA
    GCATCAGCCA CTCGTGTCCG TTCTAGGAGC CTTCCACGTG AGGGTGGTCG GCTTCTGGAT
    GAGGACCAAG ATGACAGTGA TGATGATGAT GATGAAGATG ATGATGAAGA TGATGAAGAG
    GAAGATGAGG AGCTCTCTCC ATATGAAGCC GAAGACTTCC TGCCAGGTCC CGGACAGAGA
    ATTCCAAAGG ATGAGGAAGA GAGTACTGAC GACCAAGCCA TGCCAGAGCT ACAGTTTGGA
    AGTCTTAAGC GTATGCAGCA TTCAGAGAGC CCTGACCACA CATGGATTGA GAGAGGCAGA
    TCCAGACTCC CACGGAAAGT AGACATGGGG AGCTGTGAGA TTTCCTCTAG TTCAGATACT
    TTCAGCAGCC CCATACACTC TGCATCCACT ACAGGAGTGC TTGGCAGCCA GATTGACCAT
    AAAGAGGATC ACCAGTCCTC AAGTGGCAAC TGGAGTGGAA GCAGCTCCAC TTGCCCATCG
    CAGACATCCG AGACTCTTCC ACCTGCTGCC TCCCCTCCCT TGACGGGATC CTCACACTGT
    GATTCAGAAC TTTCCCTGAA TACTGGGTCC CATGTCATAG ATGACAACAC TGGTTTCATA
    ATTGATCCCT ATCACATAGA CAAGCCACAG GGACAGGGGC AACGATCCAA TTCATTTACC
    TCATCAGCTA CTGATCAGAT GGATGATGCA GGGGTCAGCA CTGCTAGTGA TGGGGAGTGG
    AACTGTGCCC AGGACCAGCA GGATCAGTCC TGCACCCAAG ACTTCAGCCC AAAGCGTTCC
    AGAGAGGATG AGAGTGGTGT CAGCTGCCCT AGTTATTCTA GTGGGGAAAC TTACGAGAGC
    TTCAGTGACC AAGCATCCGC TAGAAAATCT GAGATGCACT ACATTGTCGA CACTGAAGGA
    TTCTATTCCT CCATGCACTT TGACTCTGGT CTTAAGGGTA GCAGAAGCTA CTACAACTAT
    GCAGCCATTG ACCCCGACTG TGGCCCAAGT GGTAATATAG CACCTGGCAT GACTGAATAC
    CCTCAGCCAG AGTACAGAGC CACCAGGACA CTGGGCAGAC CCTGTCTCTC CTTAAGAAAA
    CCAAAGGCCA AGCCACCCCC ACCAAAACGT AGCTCTTCAT TAAAGGAAAC AAGCAGTGAT
    GTGAACGTTC CAGAGCAGAA CGAACCAAAG ATTACTTGTG AGTTGCCACT GCCCTTGTCT
    TCCAGGGAGA TGAAACTGCA GCTGGACTTG GCTGATTCTG CAGGGCACCT TGAGACTGCA
    GGTCTTGAAT CACTTGGTGC ATGGGGAGTG GAAAATGTTA GGGACATGCT TGACTGCTCC
    TCCCCATTCA GTTCCTCAGA CACACATTCC TTCAAAGATG AAGGTGCTGT GCAAGCAGAC
    TATGCAGACC TCTGGCTTCA CAATGACTTG AAATCAAATG ATCCTTATCG GTCCCTCTCT
    AACTCTAGCT CTGCTACAGG TACAACTGTT ATTGAATGCA TCAAGTCACC AGAAAGATCA
    GAAACGCACA CCTGTCAACC CAGGTCCAGA CCTTCTTCCC CAACTCTTCC TCCACCTGAA
    AGTGAATTTA AGCTTGCTTC TCCCGAAAAG CTGGCAGGCT TGGCGTCCCC TTCCAGTGGA
    TATTCCAGTC AGTCTGAAAC ACCTACATCT TCCTTCCCCT CTGCTTTCTT CCCAGGGCCC
    CTGTCTCCAA CCAGTGGGAA GAGGAAGCCC AAAGTCCCTG AAAGGAAGTC CTCACTTTGT
    TCCTTACAGC AGCAACAGCT TTCAGTCAGA GACCCAGGCA TTTCCTGTAG GAGAGAAACT
    GACTTCTATG CCATACCCCC AAGTCACCTT GACCTAAGTG CTCTTCACAG TAAGCACTTT
    CCTCACAGAA ATCAAATGCA CATCCTCCAC CAGAATAAGC AGAAAGCTGC CATAGCAGCA
    GCTGCTGCAG CAGAAGCTCG CAGTGCAGCA GCTGCTGCAG CAGAAGCTCG CAGTGCAGCA
    GCTGCTGCAG CAGAAGCTCG CAGTGCAGCA ACTGCAGCCG CTGCTGCCGA GGCCTGCACT
    GCAGCTACAG CTTCTACTAA AGAGAGTGCA ACAAATACAG CACCCATCTC AGCCCATTTG
    GCTATTACTC CAACGGTTCT TAGATCAGTG CAACTGCGAT CTATATGTAG ACCATCTGAT
    GGCAACCAAG GGCTTGATCA AGACAGTTCA AATCTCATAA CTCGTCCTAA GTGTCCCACA
    ATAATAACTG ATGCCCCATC ATCTAGCAAC AGGCACAACA GGAAGCCACC AGCCTACAAA
    CCCCCTGCTG CACAGGTTTG TGATTCACAG ATTCCACTGG TGGAAAACAT TGTTTTTCCA
    CAAGAAGAAG TGAGAGTGAG ACAGGAGAGG TTTGGTCCTG GTACTTACTG GGCAATGACT
    GCTTTCAGAA CTCCTGTGGA CCCTAATTGT GAAAAAGAAG TCTCTTTAAA AAGGTCATCT
    CAGTGTTTTC AGCAAGAACA AGACAGCAGA AGGTGCCTAG ATGTGCAAAA GACATTGTCA
    CCAGAGAGAC TAAAACAAGA TTTGAATCAA CTATCGCGGC CAGACCCTTC AACACAGTCC
    ATGTGTTTGG CCAGCACAAT GCCACCCCCT GCTTATAGTG AGATACAGAG GAGCAATTTT
    GTACTGTGCA ACAGTCCTGA CAAAGTGCCT GTTTCAACTC AAGAGGCATC GCATTCTTAC
    ACAATCAGCG ATGTACAAGG CAGAGAGGAG GATTATGAGA CATCAGGTGT CGCAACCAGA
    AGTGCCTCAC AGGACATAAG GGAAGAAGAG TCAACCCCAG ATACAGAGGA CTATTTCAGT
    AAAGACTCTA CTCCCAGTGA TAATTCATCC TCTCCTTTGA CCGATGACTC CAGACTTGAT
    GATGATGTTT TCCCATCTCC CAATAAACTC CGCACAACTG AAGATCTTTT TGCTATGATA
    CACAGATCTA AAAGGAAAGT GCTGGGACGC AAAGATTCAG GGGAAGTCAG TGGAAAAGGT
    CGGCCTGTTG TACCACCTGT GACCACCCCT GTAAGCAATC CTACTGTATG CCCGGTCATC
    CCTGCTCCCC CAGTTCCTTC AAACAACTCC CAGCGAGCCT CAGGACCCAT CTACAGGAGT
    GCCAAAAAGT CTAGTACATC CAGTGAGGAG TTCAAACTCC TTCTGCTTAA GAAGGGTAGT
    CGCACAGACT CAAGTTATCG CATGTCTGCT ACAGAGATCC TTAAGAGCCC CATCACACCC
    AAGACTCAAG CAGAGCTGCT GTTAGAGGCC ATGAGGCAAC CAGAGGAGAT CCCCTTACCC
    CAACTGGATT CCACCAGCAG TGGAGATCCA CTTCCAAGTC CATTCCCTAA GGCCAACAGT
    GAGGGATTCT CCCCAAAAAC ACTCACCATG TCAGCTGCCT CCAGACAAGG ACGTTCTAGA
    ATTCCACCTG CAGCGAACAG CAGTCGCTAT AGCACGCGGA GTCGGCTGTA CACTGCCCCA
    ATGCAGGCCA TATCAGAAGG AGAGACTGAG AACTCGGATG GAAGTCCACA CGATGACCGC
    TCCTCCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa43148
    Clone ID Related Accession (Same CDS sequence) XM_009306118.3 , XM_009306118.2
    Accession Version XM_009306118.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4875bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product Nance-Horan syndrome protein isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007122.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009306118.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    ATGCCTTTTG CCAAACGGAT CGTTGAGCCC CAGCTGCTGG TTAGACATCC CATCCCTAAT 
    GATGAAGGGT TACTGTTCGA GGATCTGTGC TCCATCACCA ATGTGGCTCT ATCCCGGACC
    CTGCGGCAGC TCTCCGACCT GTCCAAACAC GCTTGCTCCT TATTTCAGGA GCTTGAGAAC
    GAAATAGTGT CCACAAACCA ACGGGTTTGG GCTTTGCAGA ACAAAATAGG CAAAATTCAG
    CAAACCGCCA GTGCGCTGGA TCCAAAAATG GAGGCTGTGC GTTTCAGGAG CATCTCTATA
    GCGGTCTCGA ATCTGGACAT TGAGAGTAAA CTGACGTCGC ACTATCAGGC TCCATGGCAT
    CAGCAACACA ATCTGTTCCA CACTTGCACA CGGCCAGCGT GTCTGGAGGA GCTGCACCGA
    CATGCCAAGC TCAACCTGCG GGCGCTGCAC AGAGACCAGC AGCAGCGTCA GCGTTCAACC
    AGCAGAGAGC GCAGTCGGGT CACTATATCA ATATCTGTGG CACCGCCAAT GCCAACCTTC
    CCTTCAACTC ACAAACTCAG ACGGCGAGAG CAGAGAGGTC GACATTCACG CATGGAGAGG
    AGCGTTAGAG AGAGTGATGT GCAAACTATC CAGAGGAAGG AGAGACCAGC CAAGGATTCT
    GAATTCAAAC CTGTCCTCAG AAAGCTGCAT TTCTTATCAA CACAGAAAAG TGGAAATTTG
    GCAATACATG CTGGAACCAA CACAGAAACG GATGGAGAAC TACAAGTGAT GAGCCATAGG
    CCTAAATGTC CTGTTCCCAA TGCACCTACC ACCCTGGACA AACAGACTAA CTGGTCTAAA
    GCCCTGCCTC TTCCCACTCC AGAAGAAAGA ATAAAGAATG ACTCTCAAGT GATTTCATCC
    TGTATCATTC CAATAAATGT TTCGGGTGTT GGATTTGACA GAGAAGCCAG TGCTCGCTGC
    TCTCTTGTTC ACTCGCAATC GGTTCTACAG AGACGGCGGA AACTCAGGAG GCGAAAAACG
    ATCACTGGGA TCCCCAAACG AGTTCAACAA GACATGGATT CTGACGAATC CCCTGTTGCT
    AGAGAAAGAA CAGTGATAAT CCACGCCAAT CCACACAAGT CCCACGAGTG GCATGAGGAG
    CTCTCCTTGA GTGGCAGAGT ATTGCATACT AAGGATTCGG GCTGTCAGAC AGATGACTTC
    TTGATTACTG CTCCATCCCG AAGACGCATC CGTGCCCAAA GAGGTCAGGG AATTCCAGCG
    TCTCTCTCTC ACTCCACTGG AAACATTACC TCCCTCCCGG ATCGTTCTGA TACGGTTTAT
    GCTGCAGCAT CAGCCACTCG TGTCCGTTCT AGGAGCCTTC CACGTGAGGG TGGTCGGCTT
    CTGGATGAGG ACCAAGATGA CAGTGATGAT GATGATGATG AAGATGATGA TGAAGATGAT
    GAAGAGGAAG ATGAGGAGCT CTCTCCATAT GAAGCCGAAG ACTTCCTGCC AGGTCCCGGA
    CAGAGAATTC CAAAGGATGA GGAAGAGAGT ACTGACGACC AAGCCATGCC AGAGCTACAG
    TTTGGAAGTC TTAAGCGTAT GCAGCATTCA GAGAGCCCTG ACCACACATG GATTGAGAGA
    GGCAGATCCA GACTCCCACG GAAAGTAGAC ATGGGGAGCT GTGAGATTTC CTCTAGTTCA
    GATACTTTCA GCAGCCCCAT ACACTCTGCA TCCACTACAG GAGTGCTTGG CAGCCAGATT
    GACCATAAAG AGGATCACCA GTCCTCAAGT GGCAACTGGA GTGGAAGCAG CTCCACTTGC
    CCATCGCAGA CATCCGAGAC TCTTCCACCT GCTGCCTCCC CTCCCTTGAC GGGATCCTCA
    CACTGTGATT CAGAACTTTC CCTGAATACT GGGTCCCATG TCATAGATGA CAACACTGGT
    TTCATAATTG ATCCCTATCA CATAGACAAG CCACAGGGAC AGGGGCAACG ATCCAATTCA
    TTTACCTCAT CAGCTACTGA TCAGATGGAT GATGCAGGGG TCAGCACTGC TAGTGATGGG
    GAGTGGAACT GTGCCCAGGA CCAGCAGGAT CAGTCCTGCA CCCAAGACTT CAGCCCAAAG
    CGTTCCAGAG AGGATGAGAG TGGTGTCAGC TGCCCTAGTT ATTCTAGTGG GGAAACTTAC
    GAGAGCTTCA GTGACCAAGC ATCCGCTAGA AAATCTGAGA TGCACTACAT TGTCGACACT
    GAAGGATTCT ATTCCTCCAT GCACTTTGAC TCTGGTCTTA AGGGTAGCAG AAGCTACTAC
    AACTATGCAG CCATTGACCC CGACTGTGGC CCAAGTGGTA ATATAGCACC TGGCATGACT
    GAATACCCTC AGCCAGAGTA CAGAGCCACC AGGACACTGG GCAGACCCTG TCTCTCCTTA
    AGAAAACCAA AGGCCAAGCC ACCCCCACCA AAACGTAGCT CTTCATTAAA GGAAACAAGC
    AGTGATGTGA ACGTTCCAGA GCAGAACGAA CCAAAGATTA CTTGTGAGTT GCCACTGCCC
    TTGTCTTCCA GGGAGATGAA ACTGCAGCTG GACTTGGCTG ATTCTGCAGG GCACCTTGAG
    ACTGCAGGTC TTGAATCACT TGGTGCATGG GGAGTGGAAA ATGTTAGGGA CATGCTTGAC
    TGCTCCTCCC CATTCAGTTC CTCAGACACA CATTCCTTCA AAGATGAAGG TGCTGTGCAA
    GCAGACTATG CAGACCTCTG GCTTCACAAT GACTTGAAAT CAAATGATCC TTATCGGTCC
    CTCTCTAACT CTAGCTCTGC TACAGGTACA ACTGTTATTG AATGCATCAA GTCACCAGAA
    AGATCAGAAA CGCACACCTG TCAACCCAGG TCCAGACCTT CTTCCCCAAC TCTTCCTCCA
    CCTGAAAGTG AATTTAAGCT TGCTTCTCCC GAAAAGCTGG CAGGCTTGGC GTCCCCTTCC
    AGTGGATATT CCAGTCAGTC TGAAACACCT ACATCTTCCT TCCCCTCTGC TTTCTTCCCA
    GGGCCCCTGT CTCCAACCAG TGGGAAGAGG AAGCCCAAAG TCCCTGAAAG GAAGTCCTCA
    CTTTGTTCCT TACAGCAGCA ACAGCTTTCA GTCAGAGACC CAGGCATTTC CTGTAGGAGA
    GAAACTGACT TCTATGCCAT ACCCCCAAGT CACCTTGACC TAAGTGCTCT TCACAGTAAG
    CACTTTCCTC ACAGAAATCA AATGCACATC CTCCACCAGA ATAAGCAGAA AGCTGCCATA
    GCAGCAGCTG CTGCAGCAGA AGCTCGCAGT GCAGCAGCTG CTGCAGCAGA AGCTCGCAGT
    GCAGCAGCTG CTGCAGCAGA AGCTCGCAGT GCAGCAACTG CAGCCGCTGC TGCCGAGGCC
    TGCACTGCAG CTACAGCTTC TACTAAAGAG AGTGCAACAA ATACAGCACC CATCTCAGCC
    CATTTGGCTA TTACTCCAAC GGTTCTTAGA TCAGTGCAAC TGCGATCTAT ATGTAGACCA
    TCTGATGGCA ACCAAGGGCT TGATCAAGAC AGTTCAAATC TCATAACTCG TCCTAAGTGT
    CCCACAATAA TAACTGATGC CCCATCATCT AGCAACAGGC ACAACAGGAA GCCACCAGCC
    TACAAACCCC CTGCTGCACA GGTTTGTGAT TCACAGATTC CACTGGTGGA AAACATTGTT
    TTTCCACAAG AAGAAGTGAG AGTGAGACAG GAGAGGTTTG GTCCTGGTAC TTACTGGGCA
    ATGACTGCTT TCAGAACTCC TGTGGACCCT AATTGTGAAA AAGAAGTCTC TTTAAAAAGG
    TCATCTCAGT GTTTTCAGCA AGAACAAGAC AGCAGAAGGT GCCTAGATGT GCAAAAGACA
    TTGTCACCAG AGAGACTAAA ACAAGATTTG AATCAACTAT CGCGGCCAGA CCCTTCAACA
    CAGTCCATGT GTTTGGCCAG CACAATGCCA CCCCCTGCTT ATAGTGAGAT ACAGAGGAGC
    AATTTTGTAC TGTGCAACAG TCCTGACAAA GTGCCTGTTT CAACTCAAGA GGCATCGCAT
    TCTTACACAA TCAGCGATGT ACAAGGCAGA GAGGAGGATT ATGAGACATC AGGTGTCGCA
    ACCAGAAGTG CCTCACAGGA CATAAGGGAA GAAGAGTCAA CCCCAGATAC AGAGGACTAT
    TTCAGTAAAG ACTCTACTCC CAGTGATAAT TCATCCTCTC CTTTGACCGA TGACTCCAGA
    CTTGATGATG ATGTTTTCCC ATCTCCCAAT AAACTCCGCA CAACTGAAGA TCTTTTTGCT
    ATGATACACA GATCTAAAAG GAAAGTGCTG GGACGCAAAG ATTCAGGGGA AGTCAGTGGA
    AAAGGTCGGC CTGTTGTACC ACCTGTGACC ACCCCTGTAA GCAATCCTAC TGTATGCCCG
    GTCATCCCTG CTCCCCCAGT TCCTTCAAAC AACTCCCAGC GAGCCTCAGG ACCCATCTAC
    AGGAGTGCCA AAAAGTCTAG TACATCCAGT GAGGAGTTCA AACTCCTTCT GCTTAAGAAG
    GGTAGTCGCA CAGACTCAAG TTATCGCATG TCTGCTACAG AGATCCTTAA GAGCCCCATC
    ACACCCAAGA CTCAAGCAGA GCTGCTGTTA GAGGCCATGA GGCAACCAGA GGAGATCCCC
    TTACCCCAAC TGGATTCCAC CAGCAGTGGA GATCCACTTC CAAGTCCATT CCCTAAGGCC
    AACAGTGAGG GATTCTCCCC AAAAACACTC ACCATGTCAG CTGCCTCCAG ACAAGGACGT
    TCTAGAATTC CACCTGCAGC GAACAGCAGT CGCTATAGCA CGCGGAGTCG GCTGTACACT
    GCCCCAATGC AGGCCATATC AGAAGGAGAG ACTGAGAACT CGGATGGAAG TCCACACGAT
    GACCGCTCCT CCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_009304393.1
    CDS321..5195
    Translation

    Target ORF information:

    RefSeq Version XM_009306118.3
    Organism Danio rerio(zebrafish)
    Definition Danio rerio Nance-Horan syndrome b (congenital cataracts and dental anomalies) (nhsb), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_009306118.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    ATGCCTTTTG CCAAACGGAT CGTTGAGCCC CAGCTGCTGG TTAGACATCC CATCCCTAAT 
    GATGAAGGGT TACTGTTCGA GGATCTGTGC TCCATCACCA ATGTGGCTCT ATCCCGGACC
    CTGCGGCAGC TCTCCGACCT GTCCAAACAC GCTTGCTCCT TATTTCAGGA GCTTGAGAAC
    GAAATAGTGT CCACAAACCA ACGGGTTTGG GCTTTGCAGA ACAAAATAGG CAAAATTCAG
    CAAACCGCCA GTGCGCTGGA TCCAAAAATG GAGGCTGTGC GTTTCAGGAG CATCTCTATA
    GCGGTCTCGA ATCTGGACAT TGAGAGTAAA CTGACGTCGC ACTATCAGGC TCCATGGCAT
    CAGCAACACA ATCTGTTCCA CACTTGCACA CGGCCAGCGT GTCTGGAGGA GCTGCACCGA
    CATGCCAAGC TCAACCTGCG GGCGCTGCAC AGAGACCAGC AGCAGCGTCA GCGTTCAACC
    AGCAGAGAGC GCAGTCGGGT CACTATATCA ATATCTGTGG CACCGCCAAT GCCAACCTTC
    CCTTCAACTC ACAAACTCAG ACGGCGAGAG CAGAGAGGTC GACATTCACG CATGGAGAGG
    AGCGTTAGAG AGAGTGATGT GCAAACTATC CAGAGGAAGG AGAGACCAGC CAAGGATTCT
    GAATTCAAAC CTGTCCTCAG AAAGCTGCAT TTCTTATCAA CACAGAAAAG TGGAAATTTG
    GCAATACATG CTGGAACCAA CACAGAAACG GATGGAGAAC TACAAGTGAT GAGCCATAGG
    CCTAAATGTC CTGTTCCCAA TGCACCTACC ACCCTGGACA AACAGACTAA CTGGTCTAAA
    GCCCTGCCTC TTCCCACTCC AGAAGAAAGA ATAAAGAATG ACTCTCAAGT GATTTCATCC
    TGTATCATTC CAATAAATGT TTCGGGTGTT GGATTTGACA GAGAAGCCAG TGCTCGCTGC
    TCTCTTGTTC ACTCGCAATC GGTTCTACAG AGACGGCGGA AACTCAGGAG GCGAAAAACG
    ATCACTGGGA TCCCCAAACG AGTTCAACAA GACATGGATT CTGACGAATC CCCTGTTGCT
    AGAGAAAGAA CAGTGATAAT CCACGCCAAT CCACACAAGT CCCACGAGTG GCATGAGGAG
    CTCTCCTTGA GTGGCAGAGT ATTGCATACT AAGGATTCGG GCTGTCAGAC AGATGACTTC
    TTGATTACTG CTCCATCCCG AAGACGCATC CGTGCCCAAA GAGGTCAGGG AATTCCAGCG
    TCTCTCTCTC ACTCCACTGG AAACATTACC TCCCTCCCGG ATCGTTCTGA TACGGTTTAT
    GCTGCAGCAT CAGCCACTCG TGTCCGTTCT AGGAGCCTTC CACGTGAGGG TGGTCGGCTT
    CTGGATGAGG ACCAAGATGA CAGTGATGAT GATGATGATG AAGATGATGA TGAAGATGAT
    GAAGAGGAAG ATGAGGAGCT CTCTCCATAT GAAGCCGAAG ACTTCCTGCC AGGTCCCGGA
    CAGAGAATTC CAAAGGATGA GGAAGAGAGT ACTGACGACC AAGCCATGCC AGAGCTACAG
    TTTGGAAGTC TTAAGCGTAT GCAGCATTCA GAGAGCCCTG ACCACACATG GATTGAGAGA
    GGCAGATCCA GACTCCCACG GAAAGTAGAC ATGGGGAGCT GTGAGATTTC CTCTAGTTCA
    GATACTTTCA GCAGCCCCAT ACACTCTGCA TCCACTACAG GAGTGCTTGG CAGCCAGATT
    GACCATAAAG AGGATCACCA GTCCTCAAGT GGCAACTGGA GTGGAAGCAG CTCCACTTGC
    CCATCGCAGA CATCCGAGAC TCTTCCACCT GCTGCCTCCC CTCCCTTGAC GGGATCCTCA
    CACTGTGATT CAGAACTTTC CCTGAATACT GGGTCCCATG TCATAGATGA CAACACTGGT
    TTCATAATTG ATCCCTATCA CATAGACAAG CCACAGGGAC AGGGGCAACG ATCCAATTCA
    TTTACCTCAT CAGCTACTGA TCAGATGGAT GATGCAGGGG TCAGCACTGC TAGTGATGGG
    GAGTGGAACT GTGCCCAGGA CCAGCAGGAT CAGTCCTGCA CCCAAGACTT CAGCCCAAAG
    CGTTCCAGAG AGGATGAGAG TGGTGTCAGC TGCCCTAGTT ATTCTAGTGG GGAAACTTAC
    GAGAGCTTCA GTGACCAAGC ATCCGCTAGA AAATCTGAGA TGCACTACAT TGTCGACACT
    GAAGGATTCT ATTCCTCCAT GCACTTTGAC TCTGGTCTTA AGGGTAGCAG AAGCTACTAC
    AACTATGCAG CCATTGACCC CGACTGTGGC CCAAGTGGTA ATATAGCACC TGGCATGACT
    GAATACCCTC AGCCAGAGTA CAGAGCCACC AGGACACTGG GCAGACCCTG TCTCTCCTTA
    AGAAAACCAA AGGCCAAGCC ACCCCCACCA AAACGTAGCT CTTCATTAAA GGAAACAAGC
    AGTGATGTGA ACGTTCCAGA GCAGAACGAA CCAAAGATTA CTTGTGAGTT GCCACTGCCC
    TTGTCTTCCA GGGAGATGAA ACTGCAGCTG GACTTGGCTG ATTCTGCAGG GCACCTTGAG
    ACTGCAGGTC TTGAATCACT TGGTGCATGG GGAGTGGAAA ATGTTAGGGA CATGCTTGAC
    TGCTCCTCCC CATTCAGTTC CTCAGACACA CATTCCTTCA AAGATGAAGG TGCTGTGCAA
    GCAGACTATG CAGACCTCTG GCTTCACAAT GACTTGAAAT CAAATGATCC TTATCGGTCC
    CTCTCTAACT CTAGCTCTGC TACAGGTACA ACTGTTATTG AATGCATCAA GTCACCAGAA
    AGATCAGAAA CGCACACCTG TCAACCCAGG TCCAGACCTT CTTCCCCAAC TCTTCCTCCA
    CCTGAAAGTG AATTTAAGCT TGCTTCTCCC GAAAAGCTGG CAGGCTTGGC GTCCCCTTCC
    AGTGGATATT CCAGTCAGTC TGAAACACCT ACATCTTCCT TCCCCTCTGC TTTCTTCCCA
    GGGCCCCTGT CTCCAACCAG TGGGAAGAGG AAGCCCAAAG TCCCTGAAAG GAAGTCCTCA
    CTTTGTTCCT TACAGCAGCA ACAGCTTTCA GTCAGAGACC CAGGCATTTC CTGTAGGAGA
    GAAACTGACT TCTATGCCAT ACCCCCAAGT CACCTTGACC TAAGTGCTCT TCACAGTAAG
    CACTTTCCTC ACAGAAATCA AATGCACATC CTCCACCAGA ATAAGCAGAA AGCTGCCATA
    GCAGCAGCTG CTGCAGCAGA AGCTCGCAGT GCAGCAGCTG CTGCAGCAGA AGCTCGCAGT
    GCAGCAGCTG CTGCAGCAGA AGCTCGCAGT GCAGCAACTG CAGCCGCTGC TGCCGAGGCC
    TGCACTGCAG CTACAGCTTC TACTAAAGAG AGTGCAACAA ATACAGCACC CATCTCAGCC
    CATTTGGCTA TTACTCCAAC GGTTCTTAGA TCAGTGCAAC TGCGATCTAT ATGTAGACCA
    TCTGATGGCA ACCAAGGGCT TGATCAAGAC AGTTCAAATC TCATAACTCG TCCTAAGTGT
    CCCACAATAA TAACTGATGC CCCATCATCT AGCAACAGGC ACAACAGGAA GCCACCAGCC
    TACAAACCCC CTGCTGCACA GGTTTGTGAT TCACAGATTC CACTGGTGGA AAACATTGTT
    TTTCCACAAG AAGAAGTGAG AGTGAGACAG GAGAGGTTTG GTCCTGGTAC TTACTGGGCA
    ATGACTGCTT TCAGAACTCC TGTGGACCCT AATTGTGAAA AAGAAGTCTC TTTAAAAAGG
    TCATCTCAGT GTTTTCAGCA AGAACAAGAC AGCAGAAGGT GCCTAGATGT GCAAAAGACA
    TTGTCACCAG AGAGACTAAA ACAAGATTTG AATCAACTAT CGCGGCCAGA CCCTTCAACA
    CAGTCCATGT GTTTGGCCAG CACAATGCCA CCCCCTGCTT ATAGTGAGAT ACAGAGGAGC
    AATTTTGTAC TGTGCAACAG TCCTGACAAA GTGCCTGTTT CAACTCAAGA GGCATCGCAT
    TCTTACACAA TCAGCGATGT ACAAGGCAGA GAGGAGGATT ATGAGACATC AGGTGTCGCA
    ACCAGAAGTG CCTCACAGGA CATAAGGGAA GAAGAGTCAA CCCCAGATAC AGAGGACTAT
    TTCAGTAAAG ACTCTACTCC CAGTGATAAT TCATCCTCTC CTTTGACCGA TGACTCCAGA
    CTTGATGATG ATGTTTTCCC ATCTCCCAAT AAACTCCGCA CAACTGAAGA TCTTTTTGCT
    ATGATACACA GATCTAAAAG GAAAGTGCTG GGACGCAAAG ATTCAGGGGA AGTCAGTGGA
    AAAGGTCGGC CTGTTGTACC ACCTGTGACC ACCCCTGTAA GCAATCCTAC TGTATGCCCG
    GTCATCCCTG CTCCCCCAGT TCCTTCAAAC AACTCCCAGC GAGCCTCAGG ACCCATCTAC
    AGGAGTGCCA AAAAGTCTAG TACATCCAGT GAGGAGTTCA AACTCCTTCT GCTTAAGAAG
    GGTAGTCGCA CAGACTCAAG TTATCGCATG TCTGCTACAG AGATCCTTAA GAGCCCCATC
    ACACCCAAGA CTCAAGCAGA GCTGCTGTTA GAGGCCATGA GGCAACCAGA GGAGATCCCC
    TTACCCCAAC TGGATTCCAC CAGCAGTGGA GATCCACTTC CAAGTCCATT CCCTAAGGCC
    AACAGTGAGG GATTCTCCCC AAAAACACTC ACCATGTCAG CTGCCTCCAG ACAAGGACGT
    TCTAGAATTC CACCTGCAGC GAACAGCAGT CGCTATAGCA CGCGGAGTCG GCTGTACACT
    GCCCCAATGC AGGCCATATC AGAAGGAGAG ACTGAGAACT CGGATGGAAG TCCACACGAT
    GACCGCTCCT CCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa43148
    Clone ID Related Accession (Same CDS sequence) XM_009306118.3 , XM_009306118.2
    Accession Version XM_009306118.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4875bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product Nance-Horan syndrome protein isoform X1
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007122.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009306118.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    ATGCCTTTTG CCAAACGGAT CGTTGAGCCC CAGCTGCTGG TTAGACATCC CATCCCTAAT 
    GATGAAGGGT TACTGTTCGA GGATCTGTGC TCCATCACCA ATGTGGCTCT ATCCCGGACC
    CTGCGGCAGC TCTCCGACCT GTCCAAACAC GCTTGCTCCT TATTTCAGGA GCTTGAGAAC
    GAAATAGTGT CCACAAACCA ACGGGTTTGG GCTTTGCAGA ACAAAATAGG CAAAATTCAG
    CAAACCGCCA GTGCGCTGGA TCCAAAAATG GAGGCTGTGC GTTTCAGGAG CATCTCTATA
    GCGGTCTCGA ATCTGGACAT TGAGAGTAAA CTGACGTCGC ACTATCAGGC TCCATGGCAT
    CAGCAACACA ATCTGTTCCA CACTTGCACA CGGCCAGCGT GTCTGGAGGA GCTGCACCGA
    CATGCCAAGC TCAACCTGCG GGCGCTGCAC AGAGACCAGC AGCAGCGTCA GCGTTCAACC
    AGCAGAGAGC GCAGTCGGGT CACTATATCA ATATCTGTGG CACCGCCAAT GCCAACCTTC
    CCTTCAACTC ACAAACTCAG ACGGCGAGAG CAGAGAGGTC GACATTCACG CATGGAGAGG
    AGCGTTAGAG AGAGTGATGT GCAAACTATC CAGAGGAAGG AGAGACCAGC CAAGGATTCT
    GAATTCAAAC CTGTCCTCAG AAAGCTGCAT TTCTTATCAA CACAGAAAAG TGGAAATTTG
    GCAATACATG CTGGAACCAA CACAGAAACG GATGGAGAAC TACAAGTGAT GAGCCATAGG
    CCTAAATGTC CTGTTCCCAA TGCACCTACC ACCCTGGACA AACAGACTAA CTGGTCTAAA
    GCCCTGCCTC TTCCCACTCC AGAAGAAAGA ATAAAGAATG ACTCTCAAGT GATTTCATCC
    TGTATCATTC CAATAAATGT TTCGGGTGTT GGATTTGACA GAGAAGCCAG TGCTCGCTGC
    TCTCTTGTTC ACTCGCAATC GGTTCTACAG AGACGGCGGA AACTCAGGAG GCGAAAAACG
    ATCACTGGGA TCCCCAAACG AGTTCAACAA GACATGGATT CTGACGAATC CCCTGTTGCT
    AGAGAAAGAA CAGTGATAAT CCACGCCAAT CCACACAAGT CCCACGAGTG GCATGAGGAG
    CTCTCCTTGA GTGGCAGAGT ATTGCATACT AAGGATTCGG GCTGTCAGAC AGATGACTTC
    TTGATTACTG CTCCATCCCG AAGACGCATC CGTGCCCAAA GAGGTCAGGG AATTCCAGCG
    TCTCTCTCTC ACTCCACTGG AAACATTACC TCCCTCCCGG ATCGTTCTGA TACGGTTTAT
    GCTGCAGCAT CAGCCACTCG TGTCCGTTCT AGGAGCCTTC CACGTGAGGG TGGTCGGCTT
    CTGGATGAGG ACCAAGATGA CAGTGATGAT GATGATGATG AAGATGATGA TGAAGATGAT
    GAAGAGGAAG ATGAGGAGCT CTCTCCATAT GAAGCCGAAG ACTTCCTGCC AGGTCCCGGA
    CAGAGAATTC CAAAGGATGA GGAAGAGAGT ACTGACGACC AAGCCATGCC AGAGCTACAG
    TTTGGAAGTC TTAAGCGTAT GCAGCATTCA GAGAGCCCTG ACCACACATG GATTGAGAGA
    GGCAGATCCA GACTCCCACG GAAAGTAGAC ATGGGGAGCT GTGAGATTTC CTCTAGTTCA
    GATACTTTCA GCAGCCCCAT ACACTCTGCA TCCACTACAG GAGTGCTTGG CAGCCAGATT
    GACCATAAAG AGGATCACCA GTCCTCAAGT GGCAACTGGA GTGGAAGCAG CTCCACTTGC
    CCATCGCAGA CATCCGAGAC TCTTCCACCT GCTGCCTCCC CTCCCTTGAC GGGATCCTCA
    CACTGTGATT CAGAACTTTC CCTGAATACT GGGTCCCATG TCATAGATGA CAACACTGGT
    TTCATAATTG ATCCCTATCA CATAGACAAG CCACAGGGAC AGGGGCAACG ATCCAATTCA
    TTTACCTCAT CAGCTACTGA TCAGATGGAT GATGCAGGGG TCAGCACTGC TAGTGATGGG
    GAGTGGAACT GTGCCCAGGA CCAGCAGGAT CAGTCCTGCA CCCAAGACTT CAGCCCAAAG
    CGTTCCAGAG AGGATGAGAG TGGTGTCAGC TGCCCTAGTT ATTCTAGTGG GGAAACTTAC
    GAGAGCTTCA GTGACCAAGC ATCCGCTAGA AAATCTGAGA TGCACTACAT TGTCGACACT
    GAAGGATTCT ATTCCTCCAT GCACTTTGAC TCTGGTCTTA AGGGTAGCAG AAGCTACTAC
    AACTATGCAG CCATTGACCC CGACTGTGGC CCAAGTGGTA ATATAGCACC TGGCATGACT
    GAATACCCTC AGCCAGAGTA CAGAGCCACC AGGACACTGG GCAGACCCTG TCTCTCCTTA
    AGAAAACCAA AGGCCAAGCC ACCCCCACCA AAACGTAGCT CTTCATTAAA GGAAACAAGC
    AGTGATGTGA ACGTTCCAGA GCAGAACGAA CCAAAGATTA CTTGTGAGTT GCCACTGCCC
    TTGTCTTCCA GGGAGATGAA ACTGCAGCTG GACTTGGCTG ATTCTGCAGG GCACCTTGAG
    ACTGCAGGTC TTGAATCACT TGGTGCATGG GGAGTGGAAA ATGTTAGGGA CATGCTTGAC
    TGCTCCTCCC CATTCAGTTC CTCAGACACA CATTCCTTCA AAGATGAAGG TGCTGTGCAA
    GCAGACTATG CAGACCTCTG GCTTCACAAT GACTTGAAAT CAAATGATCC TTATCGGTCC
    CTCTCTAACT CTAGCTCTGC TACAGGTACA ACTGTTATTG AATGCATCAA GTCACCAGAA
    AGATCAGAAA CGCACACCTG TCAACCCAGG TCCAGACCTT CTTCCCCAAC TCTTCCTCCA
    CCTGAAAGTG AATTTAAGCT TGCTTCTCCC GAAAAGCTGG CAGGCTTGGC GTCCCCTTCC
    AGTGGATATT CCAGTCAGTC TGAAACACCT ACATCTTCCT TCCCCTCTGC TTTCTTCCCA
    GGGCCCCTGT CTCCAACCAG TGGGAAGAGG AAGCCCAAAG TCCCTGAAAG GAAGTCCTCA
    CTTTGTTCCT TACAGCAGCA ACAGCTTTCA GTCAGAGACC CAGGCATTTC CTGTAGGAGA
    GAAACTGACT TCTATGCCAT ACCCCCAAGT CACCTTGACC TAAGTGCTCT TCACAGTAAG
    CACTTTCCTC ACAGAAATCA AATGCACATC CTCCACCAGA ATAAGCAGAA AGCTGCCATA
    GCAGCAGCTG CTGCAGCAGA AGCTCGCAGT GCAGCAGCTG CTGCAGCAGA AGCTCGCAGT
    GCAGCAGCTG CTGCAGCAGA AGCTCGCAGT GCAGCAACTG CAGCCGCTGC TGCCGAGGCC
    TGCACTGCAG CTACAGCTTC TACTAAAGAG AGTGCAACAA ATACAGCACC CATCTCAGCC
    CATTTGGCTA TTACTCCAAC GGTTCTTAGA TCAGTGCAAC TGCGATCTAT ATGTAGACCA
    TCTGATGGCA ACCAAGGGCT TGATCAAGAC AGTTCAAATC TCATAACTCG TCCTAAGTGT
    CCCACAATAA TAACTGATGC CCCATCATCT AGCAACAGGC ACAACAGGAA GCCACCAGCC
    TACAAACCCC CTGCTGCACA GGTTTGTGAT TCACAGATTC CACTGGTGGA AAACATTGTT
    TTTCCACAAG AAGAAGTGAG AGTGAGACAG GAGAGGTTTG GTCCTGGTAC TTACTGGGCA
    ATGACTGCTT TCAGAACTCC TGTGGACCCT AATTGTGAAA AAGAAGTCTC TTTAAAAAGG
    TCATCTCAGT GTTTTCAGCA AGAACAAGAC AGCAGAAGGT GCCTAGATGT GCAAAAGACA
    TTGTCACCAG AGAGACTAAA ACAAGATTTG AATCAACTAT CGCGGCCAGA CCCTTCAACA
    CAGTCCATGT GTTTGGCCAG CACAATGCCA CCCCCTGCTT ATAGTGAGAT ACAGAGGAGC
    AATTTTGTAC TGTGCAACAG TCCTGACAAA GTGCCTGTTT CAACTCAAGA GGCATCGCAT
    TCTTACACAA TCAGCGATGT ACAAGGCAGA GAGGAGGATT ATGAGACATC AGGTGTCGCA
    ACCAGAAGTG CCTCACAGGA CATAAGGGAA GAAGAGTCAA CCCCAGATAC AGAGGACTAT
    TTCAGTAAAG ACTCTACTCC CAGTGATAAT TCATCCTCTC CTTTGACCGA TGACTCCAGA
    CTTGATGATG ATGTTTTCCC ATCTCCCAAT AAACTCCGCA CAACTGAAGA TCTTTTTGCT
    ATGATACACA GATCTAAAAG GAAAGTGCTG GGACGCAAAG ATTCAGGGGA AGTCAGTGGA
    AAAGGTCGGC CTGTTGTACC ACCTGTGACC ACCCCTGTAA GCAATCCTAC TGTATGCCCG
    GTCATCCCTG CTCCCCCAGT TCCTTCAAAC AACTCCCAGC GAGCCTCAGG ACCCATCTAC
    AGGAGTGCCA AAAAGTCTAG TACATCCAGT GAGGAGTTCA AACTCCTTCT GCTTAAGAAG
    GGTAGTCGCA CAGACTCAAG TTATCGCATG TCTGCTACAG AGATCCTTAA GAGCCCCATC
    ACACCCAAGA CTCAAGCAGA GCTGCTGTTA GAGGCCATGA GGCAACCAGA GGAGATCCCC
    TTACCCCAAC TGGATTCCAC CAGCAGTGGA GATCCACTTC CAAGTCCATT CCCTAAGGCC
    AACAGTGAGG GATTCTCCCC AAAAACACTC ACCATGTCAG CTGCCTCCAG ACAAGGACGT
    TCTAGAATTC CACCTGCAGC GAACAGCAGT CGCTATAGCA CGCGGAGTCG GCTGTACACT
    GCCCCAATGC AGGCCATATC AGAAGGAGAG ACTGAGAACT CGGATGGAAG TCCACACGAT
    GACCGCTCCT CCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_009304393.1
    CDS291..5165
    Translation

    Target ORF information:

    RefSeq Version XM_009306118.2
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio Nance-Horan syndrome b (congenital cataracts and dental anomalies) (nhsb), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_009306118.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    ATGCCTTTTG CCAAACGGAT CGTTGAGCCC CAGCTGCTGG TTAGACATCC CATCCCTAAT 
    GATGAAGGGT TACTGTTCGA GGATCTGTGC TCCATCACCA ATGTGGCTCT ATCCCGGACC
    CTGCGGCAGC TCTCCGACCT GTCCAAACAC GCTTGCTCCT TATTTCAGGA GCTTGAGAAC
    GAAATAGTGT CCACAAACCA ACGGGTTTGG GCTTTGCAGA ACAAAATAGG CAAAATTCAG
    CAAACCGCCA GTGCGCTGGA TCCAAAAATG GAGGCTGTGC GTTTCAGGAG CATCTCTATA
    GCGGTCTCGA ATCTGGACAT TGAGAGTAAA CTGACGTCGC ACTATCAGGC TCCATGGCAT
    CAGCAACACA ATCTGTTCCA CACTTGCACA CGGCCAGCGT GTCTGGAGGA GCTGCACCGA
    CATGCCAAGC TCAACCTGCG GGCGCTGCAC AGAGACCAGC AGCAGCGTCA GCGTTCAACC
    AGCAGAGAGC GCAGTCGGGT CACTATATCA ATATCTGTGG CACCGCCAAT GCCAACCTTC
    CCTTCAACTC ACAAACTCAG ACGGCGAGAG CAGAGAGGTC GACATTCACG CATGGAGAGG
    AGCGTTAGAG AGAGTGATGT GCAAACTATC CAGAGGAAGG AGAGACCAGC CAAGGATTCT
    GAATTCAAAC CTGTCCTCAG AAAGCTGCAT TTCTTATCAA CACAGAAAAG TGGAAATTTG
    GCAATACATG CTGGAACCAA CACAGAAACG GATGGAGAAC TACAAGTGAT GAGCCATAGG
    CCTAAATGTC CTGTTCCCAA TGCACCTACC ACCCTGGACA AACAGACTAA CTGGTCTAAA
    GCCCTGCCTC TTCCCACTCC AGAAGAAAGA ATAAAGAATG ACTCTCAAGT GATTTCATCC
    TGTATCATTC CAATAAATGT TTCGGGTGTT GGATTTGACA GAGAAGCCAG TGCTCGCTGC
    TCTCTTGTTC ACTCGCAATC GGTTCTACAG AGACGGCGGA AACTCAGGAG GCGAAAAACG
    ATCACTGGGA TCCCCAAACG AGTTCAACAA GACATGGATT CTGACGAATC CCCTGTTGCT
    AGAGAAAGAA CAGTGATAAT CCACGCCAAT CCACACAAGT CCCACGAGTG GCATGAGGAG
    CTCTCCTTGA GTGGCAGAGT ATTGCATACT AAGGATTCGG GCTGTCAGAC AGATGACTTC
    TTGATTACTG CTCCATCCCG AAGACGCATC CGTGCCCAAA GAGGTCAGGG AATTCCAGCG
    TCTCTCTCTC ACTCCACTGG AAACATTACC TCCCTCCCGG ATCGTTCTGA TACGGTTTAT
    GCTGCAGCAT CAGCCACTCG TGTCCGTTCT AGGAGCCTTC CACGTGAGGG TGGTCGGCTT
    CTGGATGAGG ACCAAGATGA CAGTGATGAT GATGATGATG AAGATGATGA TGAAGATGAT
    GAAGAGGAAG ATGAGGAGCT CTCTCCATAT GAAGCCGAAG ACTTCCTGCC AGGTCCCGGA
    CAGAGAATTC CAAAGGATGA GGAAGAGAGT ACTGACGACC AAGCCATGCC AGAGCTACAG
    TTTGGAAGTC TTAAGCGTAT GCAGCATTCA GAGAGCCCTG ACCACACATG GATTGAGAGA
    GGCAGATCCA GACTCCCACG GAAAGTAGAC ATGGGGAGCT GTGAGATTTC CTCTAGTTCA
    GATACTTTCA GCAGCCCCAT ACACTCTGCA TCCACTACAG GAGTGCTTGG CAGCCAGATT
    GACCATAAAG AGGATCACCA GTCCTCAAGT GGCAACTGGA GTGGAAGCAG CTCCACTTGC
    CCATCGCAGA CATCCGAGAC TCTTCCACCT GCTGCCTCCC CTCCCTTGAC GGGATCCTCA
    CACTGTGATT CAGAACTTTC CCTGAATACT GGGTCCCATG TCATAGATGA CAACACTGGT
    TTCATAATTG ATCCCTATCA CATAGACAAG CCACAGGGAC AGGGGCAACG ATCCAATTCA
    TTTACCTCAT CAGCTACTGA TCAGATGGAT GATGCAGGGG TCAGCACTGC TAGTGATGGG
    GAGTGGAACT GTGCCCAGGA CCAGCAGGAT CAGTCCTGCA CCCAAGACTT CAGCCCAAAG
    CGTTCCAGAG AGGATGAGAG TGGTGTCAGC TGCCCTAGTT ATTCTAGTGG GGAAACTTAC
    GAGAGCTTCA GTGACCAAGC ATCCGCTAGA AAATCTGAGA TGCACTACAT TGTCGACACT
    GAAGGATTCT ATTCCTCCAT GCACTTTGAC TCTGGTCTTA AGGGTAGCAG AAGCTACTAC
    AACTATGCAG CCATTGACCC CGACTGTGGC CCAAGTGGTA ATATAGCACC TGGCATGACT
    GAATACCCTC AGCCAGAGTA CAGAGCCACC AGGACACTGG GCAGACCCTG TCTCTCCTTA
    AGAAAACCAA AGGCCAAGCC ACCCCCACCA AAACGTAGCT CTTCATTAAA GGAAACAAGC
    AGTGATGTGA ACGTTCCAGA GCAGAACGAA CCAAAGATTA CTTGTGAGTT GCCACTGCCC
    TTGTCTTCCA GGGAGATGAA ACTGCAGCTG GACTTGGCTG ATTCTGCAGG GCACCTTGAG
    ACTGCAGGTC TTGAATCACT TGGTGCATGG GGAGTGGAAA ATGTTAGGGA CATGCTTGAC
    TGCTCCTCCC CATTCAGTTC CTCAGACACA CATTCCTTCA AAGATGAAGG TGCTGTGCAA
    GCAGACTATG CAGACCTCTG GCTTCACAAT GACTTGAAAT CAAATGATCC TTATCGGTCC
    CTCTCTAACT CTAGCTCTGC TACAGGTACA ACTGTTATTG AATGCATCAA GTCACCAGAA
    AGATCAGAAA CGCACACCTG TCAACCCAGG TCCAGACCTT CTTCCCCAAC TCTTCCTCCA
    CCTGAAAGTG AATTTAAGCT TGCTTCTCCC GAAAAGCTGG CAGGCTTGGC GTCCCCTTCC
    AGTGGATATT CCAGTCAGTC TGAAACACCT ACATCTTCCT TCCCCTCTGC TTTCTTCCCA
    GGGCCCCTGT CTCCAACCAG TGGGAAGAGG AAGCCCAAAG TCCCTGAAAG GAAGTCCTCA
    CTTTGTTCCT TACAGCAGCA ACAGCTTTCA GTCAGAGACC CAGGCATTTC CTGTAGGAGA
    GAAACTGACT TCTATGCCAT ACCCCCAAGT CACCTTGACC TAAGTGCTCT TCACAGTAAG
    CACTTTCCTC ACAGAAATCA AATGCACATC CTCCACCAGA ATAAGCAGAA AGCTGCCATA
    GCAGCAGCTG CTGCAGCAGA AGCTCGCAGT GCAGCAGCTG CTGCAGCAGA AGCTCGCAGT
    GCAGCAGCTG CTGCAGCAGA AGCTCGCAGT GCAGCAACTG CAGCCGCTGC TGCCGAGGCC
    TGCACTGCAG CTACAGCTTC TACTAAAGAG AGTGCAACAA ATACAGCACC CATCTCAGCC
    CATTTGGCTA TTACTCCAAC GGTTCTTAGA TCAGTGCAAC TGCGATCTAT ATGTAGACCA
    TCTGATGGCA ACCAAGGGCT TGATCAAGAC AGTTCAAATC TCATAACTCG TCCTAAGTGT
    CCCACAATAA TAACTGATGC CCCATCATCT AGCAACAGGC ACAACAGGAA GCCACCAGCC
    TACAAACCCC CTGCTGCACA GGTTTGTGAT TCACAGATTC CACTGGTGGA AAACATTGTT
    TTTCCACAAG AAGAAGTGAG AGTGAGACAG GAGAGGTTTG GTCCTGGTAC TTACTGGGCA
    ATGACTGCTT TCAGAACTCC TGTGGACCCT AATTGTGAAA AAGAAGTCTC TTTAAAAAGG
    TCATCTCAGT GTTTTCAGCA AGAACAAGAC AGCAGAAGGT GCCTAGATGT GCAAAAGACA
    TTGTCACCAG AGAGACTAAA ACAAGATTTG AATCAACTAT CGCGGCCAGA CCCTTCAACA
    CAGTCCATGT GTTTGGCCAG CACAATGCCA CCCCCTGCTT ATAGTGAGAT ACAGAGGAGC
    AATTTTGTAC TGTGCAACAG TCCTGACAAA GTGCCTGTTT CAACTCAAGA GGCATCGCAT
    TCTTACACAA TCAGCGATGT ACAAGGCAGA GAGGAGGATT ATGAGACATC AGGTGTCGCA
    ACCAGAAGTG CCTCACAGGA CATAAGGGAA GAAGAGTCAA CCCCAGATAC AGAGGACTAT
    TTCAGTAAAG ACTCTACTCC CAGTGATAAT TCATCCTCTC CTTTGACCGA TGACTCCAGA
    CTTGATGATG ATGTTTTCCC ATCTCCCAAT AAACTCCGCA CAACTGAAGA TCTTTTTGCT
    ATGATACACA GATCTAAAAG GAAAGTGCTG GGACGCAAAG ATTCAGGGGA AGTCAGTGGA
    AAAGGTCGGC CTGTTGTACC ACCTGTGACC ACCCCTGTAA GCAATCCTAC TGTATGCCCG
    GTCATCCCTG CTCCCCCAGT TCCTTCAAAC AACTCCCAGC GAGCCTCAGG ACCCATCTAC
    AGGAGTGCCA AAAAGTCTAG TACATCCAGT GAGGAGTTCA AACTCCTTCT GCTTAAGAAG
    GGTAGTCGCA CAGACTCAAG TTATCGCATG TCTGCTACAG AGATCCTTAA GAGCCCCATC
    ACACCCAAGA CTCAAGCAGA GCTGCTGTTA GAGGCCATGA GGCAACCAGA GGAGATCCCC
    TTACCCCAAC TGGATTCCAC CAGCAGTGGA GATCCACTTC CAAGTCCATT CCCTAAGGCC
    AACAGTGAGG GATTCTCCCC AAAAACACTC ACCATGTCAG CTGCCTCCAG ACAAGGACGT
    TCTAGAATTC CACCTGCAGC GAACAGCAGT CGCTATAGCA CGCGGAGTCG GCTGTACACT
    GCCCCAATGC AGGCCATATC AGAAGGAGAG ACTGAGAACT CGGATGGAAG TCCACACGAT
    GACCGCTCCT CCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa43149
    Clone ID Related Accession (Same CDS sequence) XM_009306119.2 , XM_009306119.3
    Accession Version XM_009306119.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4872bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product Nance-Horan syndrome protein isoform X2
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007122.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009306119.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    ATGCCTTTTG CCAAACGGAT CGTTGAGCCC CAGCTGCTGG TTAGACATCC CATCCCTAAT 
    GATGAAGGGT TACTGTTCGA GGATCTGTGC TCCATCACCA ATGTGGCTCT ATCCCGGACC
    CTGCGGCAGC TCTCCGACCT GTCCAAACAC GCTTGCTCCT TATTTCAGGA GCTTGAGAAC
    GAAATAGTGT CCACAAACCA ACGGGTTTGG GCTTTGCAGA ACAAAATAGG CAAAATTCAG
    CAAACCGCCA GTGCGCTGGA TCCAAAAATG GAGGCTGTGC CGGTCTCGAA TCTGGACATT
    GAGAGTAAAC TGACGTCGCA CTATCAGGCT CCATGGCATC AGCAACACAA TCTGTTCCAC
    ACTTGCACAC GGCCAGCGTG TCTGGAGGAG CTGCACCGAC ATGCCAAGCT CAACCTGCGG
    GCGCTGCACA GAGACCAGCA GCAGCGTCAG CGTTCAACCA GCAGAGAGCG CAGTCGGGTC
    ACTATATCAA TATCTGTGGC ACCGCCAATG CCAACCTTCC CTTCAACTCA CAAACTCAGA
    CGGCGAGAGC AGAGAGGTCG ACATTCACGC ATGGAGAGGA GCGTTAGAGA GAGTGATGTG
    CAAACTATCC AGAGGAAGGA GAGACCAGCC AAGGATTCTG AATTCAAACC TGTCCTCAGA
    AAGTATCAGC GCTCCCGCTC CCCCTCCCCT GTTCAGTGTT GTTACTTCAT TCCCTGGATT
    AGAAAGGCTG GAACCAACAC AGAAACGGAT GGAGAACTAC AAGTGATGAG CCATAGGCCT
    AAATGTCCTG TTCCCAATGC ACCTACCACC CTGGACAAAC AGACTAACTG GTCTAAAGCC
    CTGCCTCTTC CCACTCCAGA AGAAAGAATA AAGAATGACT CTCAAGTGAT TTCATCCTGT
    ATCATTCCAA TAAATGTTTC GGGTGTTGGA TTTGACAGAG AAGCCAGTGC TCGCTGCTCT
    CTTGTTCACT CGCAATCGGT TCTACAGAGA CGGCGGAAAC TCAGGAGGCG AAAAACGATC
    ACTGGGATCC CCAAACGAGT TCAACAAGAC ATGGATTCTG ACGAATCCCC TGTTGCTAGA
    GAAAGAACAG TGATAATCCA CGCCAATCCA CACAAGTCCC ACGAGTGGCA TGAGGAGCTC
    TCCTTGAGTG GCAGAGTATT GCATACTAAG GATTCGGGCT GTCAGACAGA TGACTTCTTG
    ATTACTGCTC CATCCCGAAG ACGCATCCGT GCCCAAAGAG GTCAGGGAAT TCCAGCGTCT
    CTCTCTCACT CCACTGGAAA CATTACCTCC CTCCCGGATC GTTCTGATAC GGTTTATGCT
    GCAGCATCAG CCACTCGTGT CCGTTCTAGG AGCCTTCCAC GTGAGGGTGG TCGGCTTCTG
    GATGAGGACC AAGATGACAG TGATGATGAT GATGATGAAG ATGATGATGA AGATGATGAA
    GAGGAAGATG AGGAGCTCTC TCCATATGAA GCCGAAGACT TCCTGCCAGG TCCCGGACAG
    AGAATTCCAA AGGATGAGGA AGAGAGTACT GACGACCAAG CCATGCCAGA GCTACAGTTT
    GGAAGTCTTA AGCGTATGCA GCATTCAGAG AGCCCTGACC ACACATGGAT TGAGAGAGGC
    AGATCCAGAC TCCCACGGAA AGTAGACATG GGGAGCTGTG AGATTTCCTC TAGTTCAGAT
    ACTTTCAGCA GCCCCATACA CTCTGCATCC ACTACAGGAG TGCTTGGCAG CCAGATTGAC
    CATAAAGAGG ATCACCAGTC CTCAAGTGGC AACTGGAGTG GAAGCAGCTC CACTTGCCCA
    TCGCAGACAT CCGAGACTCT TCCACCTGCT GCCTCCCCTC CCTTGACGGG ATCCTCACAC
    TGTGATTCAG AACTTTCCCT GAATACTGGG TCCCATGTCA TAGATGACAA CACTGGTTTC
    ATAATTGATC CCTATCACAT AGACAAGCCA CAGGGACAGG GGCAACGATC CAATTCATTT
    ACCTCATCAG CTACTGATCA GATGGATGAT GCAGGGGTCA GCACTGCTAG TGATGGGGAG
    TGGAACTGTG CCCAGGACCA GCAGGATCAG TCCTGCACCC AAGACTTCAG CCCAAAGCGT
    TCCAGAGAGG ATGAGAGTGG TGTCAGCTGC CCTAGTTATT CTAGTGGGGA AACTTACGAG
    AGCTTCAGTG ACCAAGCATC CGCTAGAAAA TCTGAGATGC ACTACATTGT CGACACTGAA
    GGATTCTATT CCTCCATGCA CTTTGACTCT GGTCTTAAGG GTAGCAGAAG CTACTACAAC
    TATGCAGCCA TTGACCCCGA CTGTGGCCCA AGTGGTAATA TAGCACCTGG CATGACTGAA
    TACCCTCAGC CAGAGTACAG AGCCACCAGG ACACTGGGCA GACCCTGTCT CTCCTTAAGA
    AAACCAAAGG CCAAGCCACC CCCACCAAAA CGTAGCTCTT CATTAAAGGA AACAAGCAGT
    GATGTGAACG TTCCAGAGCA GAACGAACCA AAGATTACTT GTGAGTTGCC ACTGCCCTTG
    TCTTCCAGGG AGATGAAACT GCAGCTGGAC TTGGCTGATT CTGCAGGGCA CCTTGAGACT
    GCAGGTCTTG AATCACTTGG TGCATGGGGA GTGGAAAATG TTAGGGACAT GCTTGACTGC
    TCCTCCCCAT TCAGTTCCTC AGACACACAT TCCTTCAAAG ATGAAGGTGC TGTGCAAGCA
    GACTATGCAG ACCTCTGGCT TCACAATGAC TTGAAATCAA ATGATCCTTA TCGGTCCCTC
    TCTAACTCTA GCTCTGCTAC AGGTACAACT GTTATTGAAT GCATCAAGTC ACCAGAAAGA
    TCAGAAACGC ACACCTGTCA ACCCAGGTCC AGACCTTCTT CCCCAACTCT TCCTCCACCT
    GAAAGTGAAT TTAAGCTTGC TTCTCCCGAA AAGCTGGCAG GCTTGGCGTC CCCTTCCAGT
    GGATATTCCA GTCAGTCTGA AACACCTACA TCTTCCTTCC CCTCTGCTTT CTTCCCAGGG
    CCCCTGTCTC CAACCAGTGG GAAGAGGAAG CCCAAAGTCC CTGAAAGGAA GTCCTCACTT
    TGTTCCTTAC AGCAGCAACA GCTTTCAGTC AGAGACCCAG GCATTTCCTG TAGGAGAGAA
    ACTGACTTCT ATGCCATACC CCCAAGTCAC CTTGACCTAA GTGCTCTTCA CAGTAAGCAC
    TTTCCTCACA GAAATCAAAT GCACATCCTC CACCAGAATA AGCAGAAAGC TGCCATAGCA
    GCAGCTGCTG CAGCAGAAGC TCGCAGTGCA GCAGCTGCTG CAGCAGAAGC TCGCAGTGCA
    GCAGCTGCTG CAGCAGAAGC TCGCAGTGCA GCAACTGCAG CCGCTGCTGC CGAGGCCTGC
    ACTGCAGCTA CAGCTTCTAC TAAAGAGAGT GCAACAAATA CAGCACCCAT CTCAGCCCAT
    TTGGCTATTA CTCCAACGGT TCTTAGATCA GTGCAACTGC GATCTATATG TAGACCATCT
    GATGGCAACC AAGGGCTTGA TCAAGACAGT TCAAATCTCA TAACTCGTCC TAAGTGTCCC
    ACAATAATAA CTGATGCCCC ATCATCTAGC AACAGGCACA ACAGGAAGCC ACCAGCCTAC
    AAACCCCCTG CTGCACAGGT TTGTGATTCA CAGATTCCAC TGGTGGAAAA CATTGTTTTT
    CCACAAGAAG AAGTGAGAGT GAGACAGGAG AGGTTTGGTC CTGGTACTTA CTGGGCAATG
    ACTGCTTTCA GAACTCCTGT GGACCCTAAT TGTGAAAAAG AAGTCTCTTT AAAAAGGTCA
    TCTCAGTGTT TTCAGCAAGA ACAAGACAGC AGAAGGTGCC TAGATGTGCA AAAGACATTG
    TCACCAGAGA GACTAAAACA AGATTTGAAT CAACTATCGC GGCCAGACCC TTCAACACAG
    TCCATGTGTT TGGCCAGCAC AATGCCACCC CCTGCTTATA GTGAGATACA GAGGAGCAAT
    TTTGTACTGT GCAACAGTCC TGACAAAGTG CCTGTTTCAA CTCAAGAGGC ATCGCATTCT
    TACACAATCA GCGATGTACA AGGCAGAGAG GAGGATTATG AGACATCAGG TGTCGCAACC
    AGAAGTGCCT CACAGGACAT AAGGGAAGAA GAGTCAACCC CAGATACAGA GGACTATTTC
    AGTAAAGACT CTACTCCCAG TGATAATTCA TCCTCTCCTT TGACCGATGA CTCCAGACTT
    GATGATGATG TTTTCCCATC TCCCAATAAA CTCCGCACAA CTGAAGATCT TTTTGCTATG
    ATACACAGAT CTAAAAGGAA AGTGCTGGGA CGCAAAGATT CAGGGGAAGT CAGTGGAAAA
    GGTCGGCCTG TTGTACCACC TGTGACCACC CCTGTAAGCA ATCCTACTGT ATGCCCGGTC
    ATCCCTGCTC CCCCAGTTCC TTCAAACAAC TCCCAGCGAG CCTCAGGACC CATCTACAGG
    AGTGCCAAAA AGTCTAGTAC ATCCAGTGAG GAGTTCAAAC TCCTTCTGCT TAAGAAGGGT
    AGTCGCACAG ACTCAAGTTA TCGCATGTCT GCTACAGAGA TCCTTAAGAG CCCCATCACA
    CCCAAGACTC AAGCAGAGCT GCTGTTAGAG GCCATGAGGC AACCAGAGGA GATCCCCTTA
    CCCCAACTGG ATTCCACCAG CAGTGGAGAT CCACTTCCAA GTCCATTCCC TAAGGCCAAC
    AGTGAGGGAT TCTCCCCAAA AACACTCACC ATGTCAGCTG CCTCCAGACA AGGACGTTCT
    AGAATTCCAC CTGCAGCGAA CAGCAGTCGC TATAGCACGC GGAGTCGGCT GTACACTGCC
    CCAATGCAGG CCATATCAGA AGGAGAGACT GAGAACTCGG ATGGAAGTCC ACACGATGAC
    CGCTCCTCCT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_009304394.1
    CDS291..5162
    Translation

    Target ORF information:

    RefSeq Version XM_009306119.2
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio Nance-Horan syndrome b (congenital cataracts and dental anomalies) (nhsb), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_009306119.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    ATGCCTTTTG CCAAACGGAT CGTTGAGCCC CAGCTGCTGG TTAGACATCC CATCCCTAAT 
    GATGAAGGGT TACTGTTCGA GGATCTGTGC TCCATCACCA ATGTGGCTCT ATCCCGGACC
    CTGCGGCAGC TCTCCGACCT GTCCAAACAC GCTTGCTCCT TATTTCAGGA GCTTGAGAAC
    GAAATAGTGT CCACAAACCA ACGGGTTTGG GCTTTGCAGA ACAAAATAGG CAAAATTCAG
    CAAACCGCCA GTGCGCTGGA TCCAAAAATG GAGGCTGTGC CGGTCTCGAA TCTGGACATT
    GAGAGTAAAC TGACGTCGCA CTATCAGGCT CCATGGCATC AGCAACACAA TCTGTTCCAC
    ACTTGCACAC GGCCAGCGTG TCTGGAGGAG CTGCACCGAC ATGCCAAGCT CAACCTGCGG
    GCGCTGCACA GAGACCAGCA GCAGCGTCAG CGTTCAACCA GCAGAGAGCG CAGTCGGGTC
    ACTATATCAA TATCTGTGGC ACCGCCAATG CCAACCTTCC CTTCAACTCA CAAACTCAGA
    CGGCGAGAGC AGAGAGGTCG ACATTCACGC ATGGAGAGGA GCGTTAGAGA GAGTGATGTG
    CAAACTATCC AGAGGAAGGA GAGACCAGCC AAGGATTCTG AATTCAAACC TGTCCTCAGA
    AAGTATCAGC GCTCCCGCTC CCCCTCCCCT GTTCAGTGTT GTTACTTCAT TCCCTGGATT
    AGAAAGGCTG GAACCAACAC AGAAACGGAT GGAGAACTAC AAGTGATGAG CCATAGGCCT
    AAATGTCCTG TTCCCAATGC ACCTACCACC CTGGACAAAC AGACTAACTG GTCTAAAGCC
    CTGCCTCTTC CCACTCCAGA AGAAAGAATA AAGAATGACT CTCAAGTGAT TTCATCCTGT
    ATCATTCCAA TAAATGTTTC GGGTGTTGGA TTTGACAGAG AAGCCAGTGC TCGCTGCTCT
    CTTGTTCACT CGCAATCGGT TCTACAGAGA CGGCGGAAAC TCAGGAGGCG AAAAACGATC
    ACTGGGATCC CCAAACGAGT TCAACAAGAC ATGGATTCTG ACGAATCCCC TGTTGCTAGA
    GAAAGAACAG TGATAATCCA CGCCAATCCA CACAAGTCCC ACGAGTGGCA TGAGGAGCTC
    TCCTTGAGTG GCAGAGTATT GCATACTAAG GATTCGGGCT GTCAGACAGA TGACTTCTTG
    ATTACTGCTC CATCCCGAAG ACGCATCCGT GCCCAAAGAG GTCAGGGAAT TCCAGCGTCT
    CTCTCTCACT CCACTGGAAA CATTACCTCC CTCCCGGATC GTTCTGATAC GGTTTATGCT
    GCAGCATCAG CCACTCGTGT CCGTTCTAGG AGCCTTCCAC GTGAGGGTGG TCGGCTTCTG
    GATGAGGACC AAGATGACAG TGATGATGAT GATGATGAAG ATGATGATGA AGATGATGAA
    GAGGAAGATG AGGAGCTCTC TCCATATGAA GCCGAAGACT TCCTGCCAGG TCCCGGACAG
    AGAATTCCAA AGGATGAGGA AGAGAGTACT GACGACCAAG CCATGCCAGA GCTACAGTTT
    GGAAGTCTTA AGCGTATGCA GCATTCAGAG AGCCCTGACC ACACATGGAT TGAGAGAGGC
    AGATCCAGAC TCCCACGGAA AGTAGACATG GGGAGCTGTG AGATTTCCTC TAGTTCAGAT
    ACTTTCAGCA GCCCCATACA CTCTGCATCC ACTACAGGAG TGCTTGGCAG CCAGATTGAC
    CATAAAGAGG ATCACCAGTC CTCAAGTGGC AACTGGAGTG GAAGCAGCTC CACTTGCCCA
    TCGCAGACAT CCGAGACTCT TCCACCTGCT GCCTCCCCTC CCTTGACGGG ATCCTCACAC
    TGTGATTCAG AACTTTCCCT GAATACTGGG TCCCATGTCA TAGATGACAA CACTGGTTTC
    ATAATTGATC CCTATCACAT AGACAAGCCA CAGGGACAGG GGCAACGATC CAATTCATTT
    ACCTCATCAG CTACTGATCA GATGGATGAT GCAGGGGTCA GCACTGCTAG TGATGGGGAG
    TGGAACTGTG CCCAGGACCA GCAGGATCAG TCCTGCACCC AAGACTTCAG CCCAAAGCGT
    TCCAGAGAGG ATGAGAGTGG TGTCAGCTGC CCTAGTTATT CTAGTGGGGA AACTTACGAG
    AGCTTCAGTG ACCAAGCATC CGCTAGAAAA TCTGAGATGC ACTACATTGT CGACACTGAA
    GGATTCTATT CCTCCATGCA CTTTGACTCT GGTCTTAAGG GTAGCAGAAG CTACTACAAC
    TATGCAGCCA TTGACCCCGA CTGTGGCCCA AGTGGTAATA TAGCACCTGG CATGACTGAA
    TACCCTCAGC CAGAGTACAG AGCCACCAGG ACACTGGGCA GACCCTGTCT CTCCTTAAGA
    AAACCAAAGG CCAAGCCACC CCCACCAAAA CGTAGCTCTT CATTAAAGGA AACAAGCAGT
    GATGTGAACG TTCCAGAGCA GAACGAACCA AAGATTACTT GTGAGTTGCC ACTGCCCTTG
    TCTTCCAGGG AGATGAAACT GCAGCTGGAC TTGGCTGATT CTGCAGGGCA CCTTGAGACT
    GCAGGTCTTG AATCACTTGG TGCATGGGGA GTGGAAAATG TTAGGGACAT GCTTGACTGC
    TCCTCCCCAT TCAGTTCCTC AGACACACAT TCCTTCAAAG ATGAAGGTGC TGTGCAAGCA
    GACTATGCAG ACCTCTGGCT TCACAATGAC TTGAAATCAA ATGATCCTTA TCGGTCCCTC
    TCTAACTCTA GCTCTGCTAC AGGTACAACT GTTATTGAAT GCATCAAGTC ACCAGAAAGA
    TCAGAAACGC ACACCTGTCA ACCCAGGTCC AGACCTTCTT CCCCAACTCT TCCTCCACCT
    GAAAGTGAAT TTAAGCTTGC TTCTCCCGAA AAGCTGGCAG GCTTGGCGTC CCCTTCCAGT
    GGATATTCCA GTCAGTCTGA AACACCTACA TCTTCCTTCC CCTCTGCTTT CTTCCCAGGG
    CCCCTGTCTC CAACCAGTGG GAAGAGGAAG CCCAAAGTCC CTGAAAGGAA GTCCTCACTT
    TGTTCCTTAC AGCAGCAACA GCTTTCAGTC AGAGACCCAG GCATTTCCTG TAGGAGAGAA
    ACTGACTTCT ATGCCATACC CCCAAGTCAC CTTGACCTAA GTGCTCTTCA CAGTAAGCAC
    TTTCCTCACA GAAATCAAAT GCACATCCTC CACCAGAATA AGCAGAAAGC TGCCATAGCA
    GCAGCTGCTG CAGCAGAAGC TCGCAGTGCA GCAGCTGCTG CAGCAGAAGC TCGCAGTGCA
    GCAGCTGCTG CAGCAGAAGC TCGCAGTGCA GCAACTGCAG CCGCTGCTGC CGAGGCCTGC
    ACTGCAGCTA CAGCTTCTAC TAAAGAGAGT GCAACAAATA CAGCACCCAT CTCAGCCCAT
    TTGGCTATTA CTCCAACGGT TCTTAGATCA GTGCAACTGC GATCTATATG TAGACCATCT
    GATGGCAACC AAGGGCTTGA TCAAGACAGT TCAAATCTCA TAACTCGTCC TAAGTGTCCC
    ACAATAATAA CTGATGCCCC ATCATCTAGC AACAGGCACA ACAGGAAGCC ACCAGCCTAC
    AAACCCCCTG CTGCACAGGT TTGTGATTCA CAGATTCCAC TGGTGGAAAA CATTGTTTTT
    CCACAAGAAG AAGTGAGAGT GAGACAGGAG AGGTTTGGTC CTGGTACTTA CTGGGCAATG
    ACTGCTTTCA GAACTCCTGT GGACCCTAAT TGTGAAAAAG AAGTCTCTTT AAAAAGGTCA
    TCTCAGTGTT TTCAGCAAGA ACAAGACAGC AGAAGGTGCC TAGATGTGCA AAAGACATTG
    TCACCAGAGA GACTAAAACA AGATTTGAAT CAACTATCGC GGCCAGACCC TTCAACACAG
    TCCATGTGTT TGGCCAGCAC AATGCCACCC CCTGCTTATA GTGAGATACA GAGGAGCAAT
    TTTGTACTGT GCAACAGTCC TGACAAAGTG CCTGTTTCAA CTCAAGAGGC ATCGCATTCT
    TACACAATCA GCGATGTACA AGGCAGAGAG GAGGATTATG AGACATCAGG TGTCGCAACC
    AGAAGTGCCT CACAGGACAT AAGGGAAGAA GAGTCAACCC CAGATACAGA GGACTATTTC
    AGTAAAGACT CTACTCCCAG TGATAATTCA TCCTCTCCTT TGACCGATGA CTCCAGACTT
    GATGATGATG TTTTCCCATC TCCCAATAAA CTCCGCACAA CTGAAGATCT TTTTGCTATG
    ATACACAGAT CTAAAAGGAA AGTGCTGGGA CGCAAAGATT CAGGGGAAGT CAGTGGAAAA
    GGTCGGCCTG TTGTACCACC TGTGACCACC CCTGTAAGCA ATCCTACTGT ATGCCCGGTC
    ATCCCTGCTC CCCCAGTTCC TTCAAACAAC TCCCAGCGAG CCTCAGGACC CATCTACAGG
    AGTGCCAAAA AGTCTAGTAC ATCCAGTGAG GAGTTCAAAC TCCTTCTGCT TAAGAAGGGT
    AGTCGCACAG ACTCAAGTTA TCGCATGTCT GCTACAGAGA TCCTTAAGAG CCCCATCACA
    CCCAAGACTC AAGCAGAGCT GCTGTTAGAG GCCATGAGGC AACCAGAGGA GATCCCCTTA
    CCCCAACTGG ATTCCACCAG CAGTGGAGAT CCACTTCCAA GTCCATTCCC TAAGGCCAAC
    AGTGAGGGAT TCTCCCCAAA AACACTCACC ATGTCAGCTG CCTCCAGACA AGGACGTTCT
    AGAATTCCAC CTGCAGCGAA CAGCAGTCGC TATAGCACGC GGAGTCGGCT GTACACTGCC
    CCAATGCAGG CCATATCAGA AGGAGAGACT GAGAACTCGG ATGGAAGTCC ACACGATGAC
    CGCTCCTCCT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa43149
    Clone ID Related Accession (Same CDS sequence) XM_009306119.2 , XM_009306119.3
    Accession Version XM_009306119.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4872bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product Nance-Horan syndrome protein isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007122.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009306119.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    ATGCCTTTTG CCAAACGGAT CGTTGAGCCC CAGCTGCTGG TTAGACATCC CATCCCTAAT 
    GATGAAGGGT TACTGTTCGA GGATCTGTGC TCCATCACCA ATGTGGCTCT ATCCCGGACC
    CTGCGGCAGC TCTCCGACCT GTCCAAACAC GCTTGCTCCT TATTTCAGGA GCTTGAGAAC
    GAAATAGTGT CCACAAACCA ACGGGTTTGG GCTTTGCAGA ACAAAATAGG CAAAATTCAG
    CAAACCGCCA GTGCGCTGGA TCCAAAAATG GAGGCTGTGC CGGTCTCGAA TCTGGACATT
    GAGAGTAAAC TGACGTCGCA CTATCAGGCT CCATGGCATC AGCAACACAA TCTGTTCCAC
    ACTTGCACAC GGCCAGCGTG TCTGGAGGAG CTGCACCGAC ATGCCAAGCT CAACCTGCGG
    GCGCTGCACA GAGACCAGCA GCAGCGTCAG CGTTCAACCA GCAGAGAGCG CAGTCGGGTC
    ACTATATCAA TATCTGTGGC ACCGCCAATG CCAACCTTCC CTTCAACTCA CAAACTCAGA
    CGGCGAGAGC AGAGAGGTCG ACATTCACGC ATGGAGAGGA GCGTTAGAGA GAGTGATGTG
    CAAACTATCC AGAGGAAGGA GAGACCAGCC AAGGATTCTG AATTCAAACC TGTCCTCAGA
    AAGTATCAGC GCTCCCGCTC CCCCTCCCCT GTTCAGTGTT GTTACTTCAT TCCCTGGATT
    AGAAAGGCTG GAACCAACAC AGAAACGGAT GGAGAACTAC AAGTGATGAG CCATAGGCCT
    AAATGTCCTG TTCCCAATGC ACCTACCACC CTGGACAAAC AGACTAACTG GTCTAAAGCC
    CTGCCTCTTC CCACTCCAGA AGAAAGAATA AAGAATGACT CTCAAGTGAT TTCATCCTGT
    ATCATTCCAA TAAATGTTTC GGGTGTTGGA TTTGACAGAG AAGCCAGTGC TCGCTGCTCT
    CTTGTTCACT CGCAATCGGT TCTACAGAGA CGGCGGAAAC TCAGGAGGCG AAAAACGATC
    ACTGGGATCC CCAAACGAGT TCAACAAGAC ATGGATTCTG ACGAATCCCC TGTTGCTAGA
    GAAAGAACAG TGATAATCCA CGCCAATCCA CACAAGTCCC ACGAGTGGCA TGAGGAGCTC
    TCCTTGAGTG GCAGAGTATT GCATACTAAG GATTCGGGCT GTCAGACAGA TGACTTCTTG
    ATTACTGCTC CATCCCGAAG ACGCATCCGT GCCCAAAGAG GTCAGGGAAT TCCAGCGTCT
    CTCTCTCACT CCACTGGAAA CATTACCTCC CTCCCGGATC GTTCTGATAC GGTTTATGCT
    GCAGCATCAG CCACTCGTGT CCGTTCTAGG AGCCTTCCAC GTGAGGGTGG TCGGCTTCTG
    GATGAGGACC AAGATGACAG TGATGATGAT GATGATGAAG ATGATGATGA AGATGATGAA
    GAGGAAGATG AGGAGCTCTC TCCATATGAA GCCGAAGACT TCCTGCCAGG TCCCGGACAG
    AGAATTCCAA AGGATGAGGA AGAGAGTACT GACGACCAAG CCATGCCAGA GCTACAGTTT
    GGAAGTCTTA AGCGTATGCA GCATTCAGAG AGCCCTGACC ACACATGGAT TGAGAGAGGC
    AGATCCAGAC TCCCACGGAA AGTAGACATG GGGAGCTGTG AGATTTCCTC TAGTTCAGAT
    ACTTTCAGCA GCCCCATACA CTCTGCATCC ACTACAGGAG TGCTTGGCAG CCAGATTGAC
    CATAAAGAGG ATCACCAGTC CTCAAGTGGC AACTGGAGTG GAAGCAGCTC CACTTGCCCA
    TCGCAGACAT CCGAGACTCT TCCACCTGCT GCCTCCCCTC CCTTGACGGG ATCCTCACAC
    TGTGATTCAG AACTTTCCCT GAATACTGGG TCCCATGTCA TAGATGACAA CACTGGTTTC
    ATAATTGATC CCTATCACAT AGACAAGCCA CAGGGACAGG GGCAACGATC CAATTCATTT
    ACCTCATCAG CTACTGATCA GATGGATGAT GCAGGGGTCA GCACTGCTAG TGATGGGGAG
    TGGAACTGTG CCCAGGACCA GCAGGATCAG TCCTGCACCC AAGACTTCAG CCCAAAGCGT
    TCCAGAGAGG ATGAGAGTGG TGTCAGCTGC CCTAGTTATT CTAGTGGGGA AACTTACGAG
    AGCTTCAGTG ACCAAGCATC CGCTAGAAAA TCTGAGATGC ACTACATTGT CGACACTGAA
    GGATTCTATT CCTCCATGCA CTTTGACTCT GGTCTTAAGG GTAGCAGAAG CTACTACAAC
    TATGCAGCCA TTGACCCCGA CTGTGGCCCA AGTGGTAATA TAGCACCTGG CATGACTGAA
    TACCCTCAGC CAGAGTACAG AGCCACCAGG ACACTGGGCA GACCCTGTCT CTCCTTAAGA
    AAACCAAAGG CCAAGCCACC CCCACCAAAA CGTAGCTCTT CATTAAAGGA AACAAGCAGT
    GATGTGAACG TTCCAGAGCA GAACGAACCA AAGATTACTT GTGAGTTGCC ACTGCCCTTG
    TCTTCCAGGG AGATGAAACT GCAGCTGGAC TTGGCTGATT CTGCAGGGCA CCTTGAGACT
    GCAGGTCTTG AATCACTTGG TGCATGGGGA GTGGAAAATG TTAGGGACAT GCTTGACTGC
    TCCTCCCCAT TCAGTTCCTC AGACACACAT TCCTTCAAAG ATGAAGGTGC TGTGCAAGCA
    GACTATGCAG ACCTCTGGCT TCACAATGAC TTGAAATCAA ATGATCCTTA TCGGTCCCTC
    TCTAACTCTA GCTCTGCTAC AGGTACAACT GTTATTGAAT GCATCAAGTC ACCAGAAAGA
    TCAGAAACGC ACACCTGTCA ACCCAGGTCC AGACCTTCTT CCCCAACTCT TCCTCCACCT
    GAAAGTGAAT TTAAGCTTGC TTCTCCCGAA AAGCTGGCAG GCTTGGCGTC CCCTTCCAGT
    GGATATTCCA GTCAGTCTGA AACACCTACA TCTTCCTTCC CCTCTGCTTT CTTCCCAGGG
    CCCCTGTCTC CAACCAGTGG GAAGAGGAAG CCCAAAGTCC CTGAAAGGAA GTCCTCACTT
    TGTTCCTTAC AGCAGCAACA GCTTTCAGTC AGAGACCCAG GCATTTCCTG TAGGAGAGAA
    ACTGACTTCT ATGCCATACC CCCAAGTCAC CTTGACCTAA GTGCTCTTCA CAGTAAGCAC
    TTTCCTCACA GAAATCAAAT GCACATCCTC CACCAGAATA AGCAGAAAGC TGCCATAGCA
    GCAGCTGCTG CAGCAGAAGC TCGCAGTGCA GCAGCTGCTG CAGCAGAAGC TCGCAGTGCA
    GCAGCTGCTG CAGCAGAAGC TCGCAGTGCA GCAACTGCAG CCGCTGCTGC CGAGGCCTGC
    ACTGCAGCTA CAGCTTCTAC TAAAGAGAGT GCAACAAATA CAGCACCCAT CTCAGCCCAT
    TTGGCTATTA CTCCAACGGT TCTTAGATCA GTGCAACTGC GATCTATATG TAGACCATCT
    GATGGCAACC AAGGGCTTGA TCAAGACAGT TCAAATCTCA TAACTCGTCC TAAGTGTCCC
    ACAATAATAA CTGATGCCCC ATCATCTAGC AACAGGCACA ACAGGAAGCC ACCAGCCTAC
    AAACCCCCTG CTGCACAGGT TTGTGATTCA CAGATTCCAC TGGTGGAAAA CATTGTTTTT
    CCACAAGAAG AAGTGAGAGT GAGACAGGAG AGGTTTGGTC CTGGTACTTA CTGGGCAATG
    ACTGCTTTCA GAACTCCTGT GGACCCTAAT TGTGAAAAAG AAGTCTCTTT AAAAAGGTCA
    TCTCAGTGTT TTCAGCAAGA ACAAGACAGC AGAAGGTGCC TAGATGTGCA AAAGACATTG
    TCACCAGAGA GACTAAAACA AGATTTGAAT CAACTATCGC GGCCAGACCC TTCAACACAG
    TCCATGTGTT TGGCCAGCAC AATGCCACCC CCTGCTTATA GTGAGATACA GAGGAGCAAT
    TTTGTACTGT GCAACAGTCC TGACAAAGTG CCTGTTTCAA CTCAAGAGGC ATCGCATTCT
    TACACAATCA GCGATGTACA AGGCAGAGAG GAGGATTATG AGACATCAGG TGTCGCAACC
    AGAAGTGCCT CACAGGACAT AAGGGAAGAA GAGTCAACCC CAGATACAGA GGACTATTTC
    AGTAAAGACT CTACTCCCAG TGATAATTCA TCCTCTCCTT TGACCGATGA CTCCAGACTT
    GATGATGATG TTTTCCCATC TCCCAATAAA CTCCGCACAA CTGAAGATCT TTTTGCTATG
    ATACACAGAT CTAAAAGGAA AGTGCTGGGA CGCAAAGATT CAGGGGAAGT CAGTGGAAAA
    GGTCGGCCTG TTGTACCACC TGTGACCACC CCTGTAAGCA ATCCTACTGT ATGCCCGGTC
    ATCCCTGCTC CCCCAGTTCC TTCAAACAAC TCCCAGCGAG CCTCAGGACC CATCTACAGG
    AGTGCCAAAA AGTCTAGTAC ATCCAGTGAG GAGTTCAAAC TCCTTCTGCT TAAGAAGGGT
    AGTCGCACAG ACTCAAGTTA TCGCATGTCT GCTACAGAGA TCCTTAAGAG CCCCATCACA
    CCCAAGACTC AAGCAGAGCT GCTGTTAGAG GCCATGAGGC AACCAGAGGA GATCCCCTTA
    CCCCAACTGG ATTCCACCAG CAGTGGAGAT CCACTTCCAA GTCCATTCCC TAAGGCCAAC
    AGTGAGGGAT TCTCCCCAAA AACACTCACC ATGTCAGCTG CCTCCAGACA AGGACGTTCT
    AGAATTCCAC CTGCAGCGAA CAGCAGTCGC TATAGCACGC GGAGTCGGCT GTACACTGCC
    CCAATGCAGG CCATATCAGA AGGAGAGACT GAGAACTCGG ATGGAAGTCC ACACGATGAC
    CGCTCCTCCT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_009304394.1
    CDS321..5192
    Translation

    Target ORF information:

    RefSeq Version XM_009306119.3
    Organism Danio rerio(zebrafish)
    Definition Danio rerio Nance-Horan syndrome b (congenital cataracts and dental anomalies) (nhsb), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_009306119.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    ATGCCTTTTG CCAAACGGAT CGTTGAGCCC CAGCTGCTGG TTAGACATCC CATCCCTAAT 
    GATGAAGGGT TACTGTTCGA GGATCTGTGC TCCATCACCA ATGTGGCTCT ATCCCGGACC
    CTGCGGCAGC TCTCCGACCT GTCCAAACAC GCTTGCTCCT TATTTCAGGA GCTTGAGAAC
    GAAATAGTGT CCACAAACCA ACGGGTTTGG GCTTTGCAGA ACAAAATAGG CAAAATTCAG
    CAAACCGCCA GTGCGCTGGA TCCAAAAATG GAGGCTGTGC CGGTCTCGAA TCTGGACATT
    GAGAGTAAAC TGACGTCGCA CTATCAGGCT CCATGGCATC AGCAACACAA TCTGTTCCAC
    ACTTGCACAC GGCCAGCGTG TCTGGAGGAG CTGCACCGAC ATGCCAAGCT CAACCTGCGG
    GCGCTGCACA GAGACCAGCA GCAGCGTCAG CGTTCAACCA GCAGAGAGCG CAGTCGGGTC
    ACTATATCAA TATCTGTGGC ACCGCCAATG CCAACCTTCC CTTCAACTCA CAAACTCAGA
    CGGCGAGAGC AGAGAGGTCG ACATTCACGC ATGGAGAGGA GCGTTAGAGA GAGTGATGTG
    CAAACTATCC AGAGGAAGGA GAGACCAGCC AAGGATTCTG AATTCAAACC TGTCCTCAGA
    AAGTATCAGC GCTCCCGCTC CCCCTCCCCT GTTCAGTGTT GTTACTTCAT TCCCTGGATT
    AGAAAGGCTG GAACCAACAC AGAAACGGAT GGAGAACTAC AAGTGATGAG CCATAGGCCT
    AAATGTCCTG TTCCCAATGC ACCTACCACC CTGGACAAAC AGACTAACTG GTCTAAAGCC
    CTGCCTCTTC CCACTCCAGA AGAAAGAATA AAGAATGACT CTCAAGTGAT TTCATCCTGT
    ATCATTCCAA TAAATGTTTC GGGTGTTGGA TTTGACAGAG AAGCCAGTGC TCGCTGCTCT
    CTTGTTCACT CGCAATCGGT TCTACAGAGA CGGCGGAAAC TCAGGAGGCG AAAAACGATC
    ACTGGGATCC CCAAACGAGT TCAACAAGAC ATGGATTCTG ACGAATCCCC TGTTGCTAGA
    GAAAGAACAG TGATAATCCA CGCCAATCCA CACAAGTCCC ACGAGTGGCA TGAGGAGCTC
    TCCTTGAGTG GCAGAGTATT GCATACTAAG GATTCGGGCT GTCAGACAGA TGACTTCTTG
    ATTACTGCTC CATCCCGAAG ACGCATCCGT GCCCAAAGAG GTCAGGGAAT TCCAGCGTCT
    CTCTCTCACT CCACTGGAAA CATTACCTCC CTCCCGGATC GTTCTGATAC GGTTTATGCT
    GCAGCATCAG CCACTCGTGT CCGTTCTAGG AGCCTTCCAC GTGAGGGTGG TCGGCTTCTG
    GATGAGGACC AAGATGACAG TGATGATGAT GATGATGAAG ATGATGATGA AGATGATGAA
    GAGGAAGATG AGGAGCTCTC TCCATATGAA GCCGAAGACT TCCTGCCAGG TCCCGGACAG
    AGAATTCCAA AGGATGAGGA AGAGAGTACT GACGACCAAG CCATGCCAGA GCTACAGTTT
    GGAAGTCTTA AGCGTATGCA GCATTCAGAG AGCCCTGACC ACACATGGAT TGAGAGAGGC
    AGATCCAGAC TCCCACGGAA AGTAGACATG GGGAGCTGTG AGATTTCCTC TAGTTCAGAT
    ACTTTCAGCA GCCCCATACA CTCTGCATCC ACTACAGGAG TGCTTGGCAG CCAGATTGAC
    CATAAAGAGG ATCACCAGTC CTCAAGTGGC AACTGGAGTG GAAGCAGCTC CACTTGCCCA
    TCGCAGACAT CCGAGACTCT TCCACCTGCT GCCTCCCCTC CCTTGACGGG ATCCTCACAC
    TGTGATTCAG AACTTTCCCT GAATACTGGG TCCCATGTCA TAGATGACAA CACTGGTTTC
    ATAATTGATC CCTATCACAT AGACAAGCCA CAGGGACAGG GGCAACGATC CAATTCATTT
    ACCTCATCAG CTACTGATCA GATGGATGAT GCAGGGGTCA GCACTGCTAG TGATGGGGAG
    TGGAACTGTG CCCAGGACCA GCAGGATCAG TCCTGCACCC AAGACTTCAG CCCAAAGCGT
    TCCAGAGAGG ATGAGAGTGG TGTCAGCTGC CCTAGTTATT CTAGTGGGGA AACTTACGAG
    AGCTTCAGTG ACCAAGCATC CGCTAGAAAA TCTGAGATGC ACTACATTGT CGACACTGAA
    GGATTCTATT CCTCCATGCA CTTTGACTCT GGTCTTAAGG GTAGCAGAAG CTACTACAAC
    TATGCAGCCA TTGACCCCGA CTGTGGCCCA AGTGGTAATA TAGCACCTGG CATGACTGAA
    TACCCTCAGC CAGAGTACAG AGCCACCAGG ACACTGGGCA GACCCTGTCT CTCCTTAAGA
    AAACCAAAGG CCAAGCCACC CCCACCAAAA CGTAGCTCTT CATTAAAGGA AACAAGCAGT
    GATGTGAACG TTCCAGAGCA GAACGAACCA AAGATTACTT GTGAGTTGCC ACTGCCCTTG
    TCTTCCAGGG AGATGAAACT GCAGCTGGAC TTGGCTGATT CTGCAGGGCA CCTTGAGACT
    GCAGGTCTTG AATCACTTGG TGCATGGGGA GTGGAAAATG TTAGGGACAT GCTTGACTGC
    TCCTCCCCAT TCAGTTCCTC AGACACACAT TCCTTCAAAG ATGAAGGTGC TGTGCAAGCA
    GACTATGCAG ACCTCTGGCT TCACAATGAC TTGAAATCAA ATGATCCTTA TCGGTCCCTC
    TCTAACTCTA GCTCTGCTAC AGGTACAACT GTTATTGAAT GCATCAAGTC ACCAGAAAGA
    TCAGAAACGC ACACCTGTCA ACCCAGGTCC AGACCTTCTT CCCCAACTCT TCCTCCACCT
    GAAAGTGAAT TTAAGCTTGC TTCTCCCGAA AAGCTGGCAG GCTTGGCGTC CCCTTCCAGT
    GGATATTCCA GTCAGTCTGA AACACCTACA TCTTCCTTCC CCTCTGCTTT CTTCCCAGGG
    CCCCTGTCTC CAACCAGTGG GAAGAGGAAG CCCAAAGTCC CTGAAAGGAA GTCCTCACTT
    TGTTCCTTAC AGCAGCAACA GCTTTCAGTC AGAGACCCAG GCATTTCCTG TAGGAGAGAA
    ACTGACTTCT ATGCCATACC CCCAAGTCAC CTTGACCTAA GTGCTCTTCA CAGTAAGCAC
    TTTCCTCACA GAAATCAAAT GCACATCCTC CACCAGAATA AGCAGAAAGC TGCCATAGCA
    GCAGCTGCTG CAGCAGAAGC TCGCAGTGCA GCAGCTGCTG CAGCAGAAGC TCGCAGTGCA
    GCAGCTGCTG CAGCAGAAGC TCGCAGTGCA GCAACTGCAG CCGCTGCTGC CGAGGCCTGC
    ACTGCAGCTA CAGCTTCTAC TAAAGAGAGT GCAACAAATA CAGCACCCAT CTCAGCCCAT
    TTGGCTATTA CTCCAACGGT TCTTAGATCA GTGCAACTGC GATCTATATG TAGACCATCT
    GATGGCAACC AAGGGCTTGA TCAAGACAGT TCAAATCTCA TAACTCGTCC TAAGTGTCCC
    ACAATAATAA CTGATGCCCC ATCATCTAGC AACAGGCACA ACAGGAAGCC ACCAGCCTAC
    AAACCCCCTG CTGCACAGGT TTGTGATTCA CAGATTCCAC TGGTGGAAAA CATTGTTTTT
    CCACAAGAAG AAGTGAGAGT GAGACAGGAG AGGTTTGGTC CTGGTACTTA CTGGGCAATG
    ACTGCTTTCA GAACTCCTGT GGACCCTAAT TGTGAAAAAG AAGTCTCTTT AAAAAGGTCA
    TCTCAGTGTT TTCAGCAAGA ACAAGACAGC AGAAGGTGCC TAGATGTGCA AAAGACATTG
    TCACCAGAGA GACTAAAACA AGATTTGAAT CAACTATCGC GGCCAGACCC TTCAACACAG
    TCCATGTGTT TGGCCAGCAC AATGCCACCC CCTGCTTATA GTGAGATACA GAGGAGCAAT
    TTTGTACTGT GCAACAGTCC TGACAAAGTG CCTGTTTCAA CTCAAGAGGC ATCGCATTCT
    TACACAATCA GCGATGTACA AGGCAGAGAG GAGGATTATG AGACATCAGG TGTCGCAACC
    AGAAGTGCCT CACAGGACAT AAGGGAAGAA GAGTCAACCC CAGATACAGA GGACTATTTC
    AGTAAAGACT CTACTCCCAG TGATAATTCA TCCTCTCCTT TGACCGATGA CTCCAGACTT
    GATGATGATG TTTTCCCATC TCCCAATAAA CTCCGCACAA CTGAAGATCT TTTTGCTATG
    ATACACAGAT CTAAAAGGAA AGTGCTGGGA CGCAAAGATT CAGGGGAAGT CAGTGGAAAA
    GGTCGGCCTG TTGTACCACC TGTGACCACC CCTGTAAGCA ATCCTACTGT ATGCCCGGTC
    ATCCCTGCTC CCCCAGTTCC TTCAAACAAC TCCCAGCGAG CCTCAGGACC CATCTACAGG
    AGTGCCAAAA AGTCTAGTAC ATCCAGTGAG GAGTTCAAAC TCCTTCTGCT TAAGAAGGGT
    AGTCGCACAG ACTCAAGTTA TCGCATGTCT GCTACAGAGA TCCTTAAGAG CCCCATCACA
    CCCAAGACTC AAGCAGAGCT GCTGTTAGAG GCCATGAGGC AACCAGAGGA GATCCCCTTA
    CCCCAACTGG ATTCCACCAG CAGTGGAGAT CCACTTCCAA GTCCATTCCC TAAGGCCAAC
    AGTGAGGGAT TCTCCCCAAA AACACTCACC ATGTCAGCTG CCTCCAGACA AGGACGTTCT
    AGAATTCCAC CTGCAGCGAA CAGCAGTCGC TATAGCACGC GGAGTCGGCT GTACACTGCC
    CCAATGCAGG CCATATCAGA AGGAGAGACT GAGAACTCGG ATGGAAGTCC ACACGATGAC
    CGCTCCTCCT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa43150
    Clone ID Related Accession (Same CDS sequence) XM_009306120.2 , XM_009306120.3
    Accession Version XM_009306120.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4830bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product Nance-Horan syndrome protein isoform X3
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007122.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009306120.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    ATGCCTTTTG CCAAACGGAT CGTTGAGCCC CAGCTGCTGG TTAGACATCC CATCCCTAAT 
    GATGAAGGGT TACTGTTCGA GGATCTGTGC TCCATCACCA ATGTGGCTCT ATCCCGGACC
    CTGCGGCAGC TCTCCGACCT GTCCAAACAC GCTTGCTCCT TATTTCAGGA GCTTGAGAAC
    GAAATAGTGT CCACAAACCA ACGGGTTTGG GCTTTGCAGA ACAAAATAGG CAAAATTCAG
    CAAACCGCCA GTGCGCTGGA TCCAAAAATG GAGGCTGTGC GTTTCAGGAG CATCTCTATA
    GCGGTCTCGA ATCTGGACAT TGAGAGTAAA CTGACGTCGC ACTATCAGGC TCCATGGCAT
    CAGCAACACA ATCTGTTCCA CACTTGCACA CGGCCAGCGT GTCTGGAGGA GCTGCACCGA
    CATGCCAAGC TCAACCTGCG GGCGCTGCAC AGAGACCAGC AGCAGCGTCA GCGTTCAACC
    AGCAGAGAGC GCAGTCGGGT CACTATATCA ATATCTGTGG CACCGCCAAT GCCAACCTTC
    CCTTCAACTC ACAAACTCAG ACGGCGAGAG CAGAGAGGTC GACATTCACG CATGGAGAGG
    AGCGTTAGAG AGAGTGATGT GCAAACTATC CAGAGGAAGG AGAGACCAGC CAAGGATTCT
    GAATTCAAAC CTGTCCTCAG AAAGGCTGGA ACCAACACAG AAACGGATGG AGAACTACAA
    GTGATGAGCC ATAGGCCTAA ATGTCCTGTT CCCAATGCAC CTACCACCCT GGACAAACAG
    ACTAACTGGT CTAAAGCCCT GCCTCTTCCC ACTCCAGAAG AAAGAATAAA GAATGACTCT
    CAAGTGATTT CATCCTGTAT CATTCCAATA AATGTTTCGG GTGTTGGATT TGACAGAGAA
    GCCAGTGCTC GCTGCTCTCT TGTTCACTCG CAATCGGTTC TACAGAGACG GCGGAAACTC
    AGGAGGCGAA AAACGATCAC TGGGATCCCC AAACGAGTTC AACAAGACAT GGATTCTGAC
    GAATCCCCTG TTGCTAGAGA AAGAACAGTG ATAATCCACG CCAATCCACA CAAGTCCCAC
    GAGTGGCATG AGGAGCTCTC CTTGAGTGGC AGAGTATTGC ATACTAAGGA TTCGGGCTGT
    CAGACAGATG ACTTCTTGAT TACTGCTCCA TCCCGAAGAC GCATCCGTGC CCAAAGAGGT
    CAGGGAATTC CAGCGTCTCT CTCTCACTCC ACTGGAAACA TTACCTCCCT CCCGGATCGT
    TCTGATACGG TTTATGCTGC AGCATCAGCC ACTCGTGTCC GTTCTAGGAG CCTTCCACGT
    GAGGGTGGTC GGCTTCTGGA TGAGGACCAA GATGACAGTG ATGATGATGA TGATGAAGAT
    GATGATGAAG ATGATGAAGA GGAAGATGAG GAGCTCTCTC CATATGAAGC CGAAGACTTC
    CTGCCAGGTC CCGGACAGAG AATTCCAAAG GATGAGGAAG AGAGTACTGA CGACCAAGCC
    ATGCCAGAGC TACAGTTTGG AAGTCTTAAG CGTATGCAGC ATTCAGAGAG CCCTGACCAC
    ACATGGATTG AGAGAGGCAG ATCCAGACTC CCACGGAAAG TAGACATGGG GAGCTGTGAG
    ATTTCCTCTA GTTCAGATAC TTTCAGCAGC CCCATACACT CTGCATCCAC TACAGGAGTG
    CTTGGCAGCC AGATTGACCA TAAAGAGGAT CACCAGTCCT CAAGTGGCAA CTGGAGTGGA
    AGCAGCTCCA CTTGCCCATC GCAGACATCC GAGACTCTTC CACCTGCTGC CTCCCCTCCC
    TTGACGGGAT CCTCACACTG TGATTCAGAA CTTTCCCTGA ATACTGGGTC CCATGTCATA
    GATGACAACA CTGGTTTCAT AATTGATCCC TATCACATAG ACAAGCCACA GGGACAGGGG
    CAACGATCCA ATTCATTTAC CTCATCAGCT ACTGATCAGA TGGATGATGC AGGGGTCAGC
    ACTGCTAGTG ATGGGGAGTG GAACTGTGCC CAGGACCAGC AGGATCAGTC CTGCACCCAA
    GACTTCAGCC CAAAGCGTTC CAGAGAGGAT GAGAGTGGTG TCAGCTGCCC TAGTTATTCT
    AGTGGGGAAA CTTACGAGAG CTTCAGTGAC CAAGCATCCG CTAGAAAATC TGAGATGCAC
    TACATTGTCG ACACTGAAGG ATTCTATTCC TCCATGCACT TTGACTCTGG TCTTAAGGGT
    AGCAGAAGCT ACTACAACTA TGCAGCCATT GACCCCGACT GTGGCCCAAG TGGTAATATA
    GCACCTGGCA TGACTGAATA CCCTCAGCCA GAGTACAGAG CCACCAGGAC ACTGGGCAGA
    CCCTGTCTCT CCTTAAGAAA ACCAAAGGCC AAGCCACCCC CACCAAAACG TAGCTCTTCA
    TTAAAGGAAA CAAGCAGTGA TGTGAACGTT CCAGAGCAGA ACGAACCAAA GATTACTTGT
    GAGTTGCCAC TGCCCTTGTC TTCCAGGGAG ATGAAACTGC AGCTGGACTT GGCTGATTCT
    GCAGGGCACC TTGAGACTGC AGGTCTTGAA TCACTTGGTG CATGGGGAGT GGAAAATGTT
    AGGGACATGC TTGACTGCTC CTCCCCATTC AGTTCCTCAG ACACACATTC CTTCAAAGAT
    GAAGGTGCTG TGCAAGCAGA CTATGCAGAC CTCTGGCTTC ACAATGACTT GAAATCAAAT
    GATCCTTATC GGTCCCTCTC TAACTCTAGC TCTGCTACAG GTACAACTGT TATTGAATGC
    ATCAAGTCAC CAGAAAGATC AGAAACGCAC ACCTGTCAAC CCAGGTCCAG ACCTTCTTCC
    CCAACTCTTC CTCCACCTGA AAGTGAATTT AAGCTTGCTT CTCCCGAAAA GCTGGCAGGC
    TTGGCGTCCC CTTCCAGTGG ATATTCCAGT CAGTCTGAAA CACCTACATC TTCCTTCCCC
    TCTGCTTTCT TCCCAGGGCC CCTGTCTCCA ACCAGTGGGA AGAGGAAGCC CAAAGTCCCT
    GAAAGGAAGT CCTCACTTTG TTCCTTACAG CAGCAACAGC TTTCAGTCAG AGACCCAGGC
    ATTTCCTGTA GGAGAGAAAC TGACTTCTAT GCCATACCCC CAAGTCACCT TGACCTAAGT
    GCTCTTCACA GTAAGCACTT TCCTCACAGA AATCAAATGC ACATCCTCCA CCAGAATAAG
    CAGAAAGCTG CCATAGCAGC AGCTGCTGCA GCAGAAGCTC GCAGTGCAGC AGCTGCTGCA
    GCAGAAGCTC GCAGTGCAGC AGCTGCTGCA GCAGAAGCTC GCAGTGCAGC AACTGCAGCC
    GCTGCTGCCG AGGCCTGCAC TGCAGCTACA GCTTCTACTA AAGAGAGTGC AACAAATACA
    GCACCCATCT CAGCCCATTT GGCTATTACT CCAACGGTTC TTAGATCAGT GCAACTGCGA
    TCTATATGTA GACCATCTGA TGGCAACCAA GGGCTTGATC AAGACAGTTC AAATCTCATA
    ACTCGTCCTA AGTGTCCCAC AATAATAACT GATGCCCCAT CATCTAGCAA CAGGCACAAC
    AGGAAGCCAC CAGCCTACAA ACCCCCTGCT GCACAGGTTT GTGATTCACA GATTCCACTG
    GTGGAAAACA TTGTTTTTCC ACAAGAAGAA GTGAGAGTGA GACAGGAGAG GTTTGGTCCT
    GGTACTTACT GGGCAATGAC TGCTTTCAGA ACTCCTGTGG ACCCTAATTG TGAAAAAGAA
    GTCTCTTTAA AAAGGTCATC TCAGTGTTTT CAGCAAGAAC AAGACAGCAG AAGGTGCCTA
    GATGTGCAAA AGACATTGTC ACCAGAGAGA CTAAAACAAG ATTTGAATCA ACTATCGCGG
    CCAGACCCTT CAACACAGTC CATGTGTTTG GCCAGCACAA TGCCACCCCC TGCTTATAGT
    GAGATACAGA GGAGCAATTT TGTACTGTGC AACAGTCCTG ACAAAGTGCC TGTTTCAACT
    CAAGAGGCAT CGCATTCTTA CACAATCAGC GATGTACAAG GCAGAGAGGA GGATTATGAG
    ACATCAGGTG TCGCAACCAG AAGTGCCTCA CAGGACATAA GGGAAGAAGA GTCAACCCCA
    GATACAGAGG ACTATTTCAG TAAAGACTCT ACTCCCAGTG ATAATTCATC CTCTCCTTTG
    ACCGATGACT CCAGACTTGA TGATGATGTT TTCCCATCTC CCAATAAACT CCGCACAACT
    GAAGATCTTT TTGCTATGAT ACACAGATCT AAAAGGAAAG TGCTGGGACG CAAAGATTCA
    GGGGAAGTCA GTGGAAAAGG TCGGCCTGTT GTACCACCTG TGACCACCCC TGTAAGCAAT
    CCTACTGTAT GCCCGGTCAT CCCTGCTCCC CCAGTTCCTT CAAACAACTC CCAGCGAGCC
    TCAGGACCCA TCTACAGGAG TGCCAAAAAG TCTAGTACAT CCAGTGAGGA GTTCAAACTC
    CTTCTGCTTA AGAAGGGTAG TCGCACAGAC TCAAGTTATC GCATGTCTGC TACAGAGATC
    CTTAAGAGCC CCATCACACC CAAGACTCAA GCAGAGCTGC TGTTAGAGGC CATGAGGCAA
    CCAGAGGAGA TCCCCTTACC CCAACTGGAT TCCACCAGCA GTGGAGATCC ACTTCCAAGT
    CCATTCCCTA AGGCCAACAG TGAGGGATTC TCCCCAAAAA CACTCACCAT GTCAGCTGCC
    TCCAGACAAG GACGTTCTAG AATTCCACCT GCAGCGAACA GCAGTCGCTA TAGCACGCGG
    AGTCGGCTGT ACACTGCCCC AATGCAGGCC ATATCAGAAG GAGAGACTGA GAACTCGGAT
    GGAAGTCCAC ACGATGACCG CTCCTCCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_009304395.1
    CDS291..5120
    Translation

    Target ORF information:

    RefSeq Version XM_009306120.2
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio Nance-Horan syndrome b (congenital cataracts and dental anomalies) (nhsb), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_009306120.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    ATGCCTTTTG CCAAACGGAT CGTTGAGCCC CAGCTGCTGG TTAGACATCC CATCCCTAAT 
    GATGAAGGGT TACTGTTCGA GGATCTGTGC TCCATCACCA ATGTGGCTCT ATCCCGGACC
    CTGCGGCAGC TCTCCGACCT GTCCAAACAC GCTTGCTCCT TATTTCAGGA GCTTGAGAAC
    GAAATAGTGT CCACAAACCA ACGGGTTTGG GCTTTGCAGA ACAAAATAGG CAAAATTCAG
    CAAACCGCCA GTGCGCTGGA TCCAAAAATG GAGGCTGTGC GTTTCAGGAG CATCTCTATA
    GCGGTCTCGA ATCTGGACAT TGAGAGTAAA CTGACGTCGC ACTATCAGGC TCCATGGCAT
    CAGCAACACA ATCTGTTCCA CACTTGCACA CGGCCAGCGT GTCTGGAGGA GCTGCACCGA
    CATGCCAAGC TCAACCTGCG GGCGCTGCAC AGAGACCAGC AGCAGCGTCA GCGTTCAACC
    AGCAGAGAGC GCAGTCGGGT CACTATATCA ATATCTGTGG CACCGCCAAT GCCAACCTTC
    CCTTCAACTC ACAAACTCAG ACGGCGAGAG CAGAGAGGTC GACATTCACG CATGGAGAGG
    AGCGTTAGAG AGAGTGATGT GCAAACTATC CAGAGGAAGG AGAGACCAGC CAAGGATTCT
    GAATTCAAAC CTGTCCTCAG AAAGGCTGGA ACCAACACAG AAACGGATGG AGAACTACAA
    GTGATGAGCC ATAGGCCTAA ATGTCCTGTT CCCAATGCAC CTACCACCCT GGACAAACAG
    ACTAACTGGT CTAAAGCCCT GCCTCTTCCC ACTCCAGAAG AAAGAATAAA GAATGACTCT
    CAAGTGATTT CATCCTGTAT CATTCCAATA AATGTTTCGG GTGTTGGATT TGACAGAGAA
    GCCAGTGCTC GCTGCTCTCT TGTTCACTCG CAATCGGTTC TACAGAGACG GCGGAAACTC
    AGGAGGCGAA AAACGATCAC TGGGATCCCC AAACGAGTTC AACAAGACAT GGATTCTGAC
    GAATCCCCTG TTGCTAGAGA AAGAACAGTG ATAATCCACG CCAATCCACA CAAGTCCCAC
    GAGTGGCATG AGGAGCTCTC CTTGAGTGGC AGAGTATTGC ATACTAAGGA TTCGGGCTGT
    CAGACAGATG ACTTCTTGAT TACTGCTCCA TCCCGAAGAC GCATCCGTGC CCAAAGAGGT
    CAGGGAATTC CAGCGTCTCT CTCTCACTCC ACTGGAAACA TTACCTCCCT CCCGGATCGT
    TCTGATACGG TTTATGCTGC AGCATCAGCC ACTCGTGTCC GTTCTAGGAG CCTTCCACGT
    GAGGGTGGTC GGCTTCTGGA TGAGGACCAA GATGACAGTG ATGATGATGA TGATGAAGAT
    GATGATGAAG ATGATGAAGA GGAAGATGAG GAGCTCTCTC CATATGAAGC CGAAGACTTC
    CTGCCAGGTC CCGGACAGAG AATTCCAAAG GATGAGGAAG AGAGTACTGA CGACCAAGCC
    ATGCCAGAGC TACAGTTTGG AAGTCTTAAG CGTATGCAGC ATTCAGAGAG CCCTGACCAC
    ACATGGATTG AGAGAGGCAG ATCCAGACTC CCACGGAAAG TAGACATGGG GAGCTGTGAG
    ATTTCCTCTA GTTCAGATAC TTTCAGCAGC CCCATACACT CTGCATCCAC TACAGGAGTG
    CTTGGCAGCC AGATTGACCA TAAAGAGGAT CACCAGTCCT CAAGTGGCAA CTGGAGTGGA
    AGCAGCTCCA CTTGCCCATC GCAGACATCC GAGACTCTTC CACCTGCTGC CTCCCCTCCC
    TTGACGGGAT CCTCACACTG TGATTCAGAA CTTTCCCTGA ATACTGGGTC CCATGTCATA
    GATGACAACA CTGGTTTCAT AATTGATCCC TATCACATAG ACAAGCCACA GGGACAGGGG
    CAACGATCCA ATTCATTTAC CTCATCAGCT ACTGATCAGA TGGATGATGC AGGGGTCAGC
    ACTGCTAGTG ATGGGGAGTG GAACTGTGCC CAGGACCAGC AGGATCAGTC CTGCACCCAA
    GACTTCAGCC CAAAGCGTTC CAGAGAGGAT GAGAGTGGTG TCAGCTGCCC TAGTTATTCT
    AGTGGGGAAA CTTACGAGAG CTTCAGTGAC CAAGCATCCG CTAGAAAATC TGAGATGCAC
    TACATTGTCG ACACTGAAGG ATTCTATTCC TCCATGCACT TTGACTCTGG TCTTAAGGGT
    AGCAGAAGCT ACTACAACTA TGCAGCCATT GACCCCGACT GTGGCCCAAG TGGTAATATA
    GCACCTGGCA TGACTGAATA CCCTCAGCCA GAGTACAGAG CCACCAGGAC ACTGGGCAGA
    CCCTGTCTCT CCTTAAGAAA ACCAAAGGCC AAGCCACCCC CACCAAAACG TAGCTCTTCA
    TTAAAGGAAA CAAGCAGTGA TGTGAACGTT CCAGAGCAGA ACGAACCAAA GATTACTTGT
    GAGTTGCCAC TGCCCTTGTC TTCCAGGGAG ATGAAACTGC AGCTGGACTT GGCTGATTCT
    GCAGGGCACC TTGAGACTGC AGGTCTTGAA TCACTTGGTG CATGGGGAGT GGAAAATGTT
    AGGGACATGC TTGACTGCTC CTCCCCATTC AGTTCCTCAG ACACACATTC CTTCAAAGAT
    GAAGGTGCTG TGCAAGCAGA CTATGCAGAC CTCTGGCTTC ACAATGACTT GAAATCAAAT
    GATCCTTATC GGTCCCTCTC TAACTCTAGC TCTGCTACAG GTACAACTGT TATTGAATGC
    ATCAAGTCAC CAGAAAGATC AGAAACGCAC ACCTGTCAAC CCAGGTCCAG ACCTTCTTCC
    CCAACTCTTC CTCCACCTGA AAGTGAATTT AAGCTTGCTT CTCCCGAAAA GCTGGCAGGC
    TTGGCGTCCC CTTCCAGTGG ATATTCCAGT CAGTCTGAAA CACCTACATC TTCCTTCCCC
    TCTGCTTTCT TCCCAGGGCC CCTGTCTCCA ACCAGTGGGA AGAGGAAGCC CAAAGTCCCT
    GAAAGGAAGT CCTCACTTTG TTCCTTACAG CAGCAACAGC TTTCAGTCAG AGACCCAGGC
    ATTTCCTGTA GGAGAGAAAC TGACTTCTAT GCCATACCCC CAAGTCACCT TGACCTAAGT
    GCTCTTCACA GTAAGCACTT TCCTCACAGA AATCAAATGC ACATCCTCCA CCAGAATAAG
    CAGAAAGCTG CCATAGCAGC AGCTGCTGCA GCAGAAGCTC GCAGTGCAGC AGCTGCTGCA
    GCAGAAGCTC GCAGTGCAGC AGCTGCTGCA GCAGAAGCTC GCAGTGCAGC AACTGCAGCC
    GCTGCTGCCG AGGCCTGCAC TGCAGCTACA GCTTCTACTA AAGAGAGTGC AACAAATACA
    GCACCCATCT CAGCCCATTT GGCTATTACT CCAACGGTTC TTAGATCAGT GCAACTGCGA
    TCTATATGTA GACCATCTGA TGGCAACCAA GGGCTTGATC AAGACAGTTC AAATCTCATA
    ACTCGTCCTA AGTGTCCCAC AATAATAACT GATGCCCCAT CATCTAGCAA CAGGCACAAC
    AGGAAGCCAC CAGCCTACAA ACCCCCTGCT GCACAGGTTT GTGATTCACA GATTCCACTG
    GTGGAAAACA TTGTTTTTCC ACAAGAAGAA GTGAGAGTGA GACAGGAGAG GTTTGGTCCT
    GGTACTTACT GGGCAATGAC TGCTTTCAGA ACTCCTGTGG ACCCTAATTG TGAAAAAGAA
    GTCTCTTTAA AAAGGTCATC TCAGTGTTTT CAGCAAGAAC AAGACAGCAG AAGGTGCCTA
    GATGTGCAAA AGACATTGTC ACCAGAGAGA CTAAAACAAG ATTTGAATCA ACTATCGCGG
    CCAGACCCTT CAACACAGTC CATGTGTTTG GCCAGCACAA TGCCACCCCC TGCTTATAGT
    GAGATACAGA GGAGCAATTT TGTACTGTGC AACAGTCCTG ACAAAGTGCC TGTTTCAACT
    CAAGAGGCAT CGCATTCTTA CACAATCAGC GATGTACAAG GCAGAGAGGA GGATTATGAG
    ACATCAGGTG TCGCAACCAG AAGTGCCTCA CAGGACATAA GGGAAGAAGA GTCAACCCCA
    GATACAGAGG ACTATTTCAG TAAAGACTCT ACTCCCAGTG ATAATTCATC CTCTCCTTTG
    ACCGATGACT CCAGACTTGA TGATGATGTT TTCCCATCTC CCAATAAACT CCGCACAACT
    GAAGATCTTT TTGCTATGAT ACACAGATCT AAAAGGAAAG TGCTGGGACG CAAAGATTCA
    GGGGAAGTCA GTGGAAAAGG TCGGCCTGTT GTACCACCTG TGACCACCCC TGTAAGCAAT
    CCTACTGTAT GCCCGGTCAT CCCTGCTCCC CCAGTTCCTT CAAACAACTC CCAGCGAGCC
    TCAGGACCCA TCTACAGGAG TGCCAAAAAG TCTAGTACAT CCAGTGAGGA GTTCAAACTC
    CTTCTGCTTA AGAAGGGTAG TCGCACAGAC TCAAGTTATC GCATGTCTGC TACAGAGATC
    CTTAAGAGCC CCATCACACC CAAGACTCAA GCAGAGCTGC TGTTAGAGGC CATGAGGCAA
    CCAGAGGAGA TCCCCTTACC CCAACTGGAT TCCACCAGCA GTGGAGATCC ACTTCCAAGT
    CCATTCCCTA AGGCCAACAG TGAGGGATTC TCCCCAAAAA CACTCACCAT GTCAGCTGCC
    TCCAGACAAG GACGTTCTAG AATTCCACCT GCAGCGAACA GCAGTCGCTA TAGCACGCGG
    AGTCGGCTGT ACACTGCCCC AATGCAGGCC ATATCAGAAG GAGAGACTGA GAACTCGGAT
    GGAAGTCCAC ACGATGACCG CTCCTCCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa43150
    Clone ID Related Accession (Same CDS sequence) XM_009306120.2 , XM_009306120.3
    Accession Version XM_009306120.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4830bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product Nance-Horan syndrome protein isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007122.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009306120.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    ATGCCTTTTG CCAAACGGAT CGTTGAGCCC CAGCTGCTGG TTAGACATCC CATCCCTAAT 
    GATGAAGGGT TACTGTTCGA GGATCTGTGC TCCATCACCA ATGTGGCTCT ATCCCGGACC
    CTGCGGCAGC TCTCCGACCT GTCCAAACAC GCTTGCTCCT TATTTCAGGA GCTTGAGAAC
    GAAATAGTGT CCACAAACCA ACGGGTTTGG GCTTTGCAGA ACAAAATAGG CAAAATTCAG
    CAAACCGCCA GTGCGCTGGA TCCAAAAATG GAGGCTGTGC GTTTCAGGAG CATCTCTATA
    GCGGTCTCGA ATCTGGACAT TGAGAGTAAA CTGACGTCGC ACTATCAGGC TCCATGGCAT
    CAGCAACACA ATCTGTTCCA CACTTGCACA CGGCCAGCGT GTCTGGAGGA GCTGCACCGA
    CATGCCAAGC TCAACCTGCG GGCGCTGCAC AGAGACCAGC AGCAGCGTCA GCGTTCAACC
    AGCAGAGAGC GCAGTCGGGT CACTATATCA ATATCTGTGG CACCGCCAAT GCCAACCTTC
    CCTTCAACTC ACAAACTCAG ACGGCGAGAG CAGAGAGGTC GACATTCACG CATGGAGAGG
    AGCGTTAGAG AGAGTGATGT GCAAACTATC CAGAGGAAGG AGAGACCAGC CAAGGATTCT
    GAATTCAAAC CTGTCCTCAG AAAGGCTGGA ACCAACACAG AAACGGATGG AGAACTACAA
    GTGATGAGCC ATAGGCCTAA ATGTCCTGTT CCCAATGCAC CTACCACCCT GGACAAACAG
    ACTAACTGGT CTAAAGCCCT GCCTCTTCCC ACTCCAGAAG AAAGAATAAA GAATGACTCT
    CAAGTGATTT CATCCTGTAT CATTCCAATA AATGTTTCGG GTGTTGGATT TGACAGAGAA
    GCCAGTGCTC GCTGCTCTCT TGTTCACTCG CAATCGGTTC TACAGAGACG GCGGAAACTC
    AGGAGGCGAA AAACGATCAC TGGGATCCCC AAACGAGTTC AACAAGACAT GGATTCTGAC
    GAATCCCCTG TTGCTAGAGA AAGAACAGTG ATAATCCACG CCAATCCACA CAAGTCCCAC
    GAGTGGCATG AGGAGCTCTC CTTGAGTGGC AGAGTATTGC ATACTAAGGA TTCGGGCTGT
    CAGACAGATG ACTTCTTGAT TACTGCTCCA TCCCGAAGAC GCATCCGTGC CCAAAGAGGT
    CAGGGAATTC CAGCGTCTCT CTCTCACTCC ACTGGAAACA TTACCTCCCT CCCGGATCGT
    TCTGATACGG TTTATGCTGC AGCATCAGCC ACTCGTGTCC GTTCTAGGAG CCTTCCACGT
    GAGGGTGGTC GGCTTCTGGA TGAGGACCAA GATGACAGTG ATGATGATGA TGATGAAGAT
    GATGATGAAG ATGATGAAGA GGAAGATGAG GAGCTCTCTC CATATGAAGC CGAAGACTTC
    CTGCCAGGTC CCGGACAGAG AATTCCAAAG GATGAGGAAG AGAGTACTGA CGACCAAGCC
    ATGCCAGAGC TACAGTTTGG AAGTCTTAAG CGTATGCAGC ATTCAGAGAG CCCTGACCAC
    ACATGGATTG AGAGAGGCAG ATCCAGACTC CCACGGAAAG TAGACATGGG GAGCTGTGAG
    ATTTCCTCTA GTTCAGATAC TTTCAGCAGC CCCATACACT CTGCATCCAC TACAGGAGTG
    CTTGGCAGCC AGATTGACCA TAAAGAGGAT CACCAGTCCT CAAGTGGCAA CTGGAGTGGA
    AGCAGCTCCA CTTGCCCATC GCAGACATCC GAGACTCTTC CACCTGCTGC CTCCCCTCCC
    TTGACGGGAT CCTCACACTG TGATTCAGAA CTTTCCCTGA ATACTGGGTC CCATGTCATA
    GATGACAACA CTGGTTTCAT AATTGATCCC TATCACATAG ACAAGCCACA GGGACAGGGG
    CAACGATCCA ATTCATTTAC CTCATCAGCT ACTGATCAGA TGGATGATGC AGGGGTCAGC
    ACTGCTAGTG ATGGGGAGTG GAACTGTGCC CAGGACCAGC AGGATCAGTC CTGCACCCAA
    GACTTCAGCC CAAAGCGTTC CAGAGAGGAT GAGAGTGGTG TCAGCTGCCC TAGTTATTCT
    AGTGGGGAAA CTTACGAGAG CTTCAGTGAC CAAGCATCCG CTAGAAAATC TGAGATGCAC
    TACATTGTCG ACACTGAAGG ATTCTATTCC TCCATGCACT TTGACTCTGG TCTTAAGGGT
    AGCAGAAGCT ACTACAACTA TGCAGCCATT GACCCCGACT GTGGCCCAAG TGGTAATATA
    GCACCTGGCA TGACTGAATA CCCTCAGCCA GAGTACAGAG CCACCAGGAC ACTGGGCAGA
    CCCTGTCTCT CCTTAAGAAA ACCAAAGGCC AAGCCACCCC CACCAAAACG TAGCTCTTCA
    TTAAAGGAAA CAAGCAGTGA TGTGAACGTT CCAGAGCAGA ACGAACCAAA GATTACTTGT
    GAGTTGCCAC TGCCCTTGTC TTCCAGGGAG ATGAAACTGC AGCTGGACTT GGCTGATTCT
    GCAGGGCACC TTGAGACTGC AGGTCTTGAA TCACTTGGTG CATGGGGAGT GGAAAATGTT
    AGGGACATGC TTGACTGCTC CTCCCCATTC AGTTCCTCAG ACACACATTC CTTCAAAGAT
    GAAGGTGCTG TGCAAGCAGA CTATGCAGAC CTCTGGCTTC ACAATGACTT GAAATCAAAT
    GATCCTTATC GGTCCCTCTC TAACTCTAGC TCTGCTACAG GTACAACTGT TATTGAATGC
    ATCAAGTCAC CAGAAAGATC AGAAACGCAC ACCTGTCAAC CCAGGTCCAG ACCTTCTTCC
    CCAACTCTTC CTCCACCTGA AAGTGAATTT AAGCTTGCTT CTCCCGAAAA GCTGGCAGGC
    TTGGCGTCCC CTTCCAGTGG ATATTCCAGT CAGTCTGAAA CACCTACATC TTCCTTCCCC
    TCTGCTTTCT TCCCAGGGCC CCTGTCTCCA ACCAGTGGGA AGAGGAAGCC CAAAGTCCCT
    GAAAGGAAGT CCTCACTTTG TTCCTTACAG CAGCAACAGC TTTCAGTCAG AGACCCAGGC
    ATTTCCTGTA GGAGAGAAAC TGACTTCTAT GCCATACCCC CAAGTCACCT TGACCTAAGT
    GCTCTTCACA GTAAGCACTT TCCTCACAGA AATCAAATGC ACATCCTCCA CCAGAATAAG
    CAGAAAGCTG CCATAGCAGC AGCTGCTGCA GCAGAAGCTC GCAGTGCAGC AGCTGCTGCA
    GCAGAAGCTC GCAGTGCAGC AGCTGCTGCA GCAGAAGCTC GCAGTGCAGC AACTGCAGCC
    GCTGCTGCCG AGGCCTGCAC TGCAGCTACA GCTTCTACTA AAGAGAGTGC AACAAATACA
    GCACCCATCT CAGCCCATTT GGCTATTACT CCAACGGTTC TTAGATCAGT GCAACTGCGA
    TCTATATGTA GACCATCTGA TGGCAACCAA GGGCTTGATC AAGACAGTTC AAATCTCATA
    ACTCGTCCTA AGTGTCCCAC AATAATAACT GATGCCCCAT CATCTAGCAA CAGGCACAAC
    AGGAAGCCAC CAGCCTACAA ACCCCCTGCT GCACAGGTTT GTGATTCACA GATTCCACTG
    GTGGAAAACA TTGTTTTTCC ACAAGAAGAA GTGAGAGTGA GACAGGAGAG GTTTGGTCCT
    GGTACTTACT GGGCAATGAC TGCTTTCAGA ACTCCTGTGG ACCCTAATTG TGAAAAAGAA
    GTCTCTTTAA AAAGGTCATC TCAGTGTTTT CAGCAAGAAC AAGACAGCAG AAGGTGCCTA
    GATGTGCAAA AGACATTGTC ACCAGAGAGA CTAAAACAAG ATTTGAATCA ACTATCGCGG
    CCAGACCCTT CAACACAGTC CATGTGTTTG GCCAGCACAA TGCCACCCCC TGCTTATAGT
    GAGATACAGA GGAGCAATTT TGTACTGTGC AACAGTCCTG ACAAAGTGCC TGTTTCAACT
    CAAGAGGCAT CGCATTCTTA CACAATCAGC GATGTACAAG GCAGAGAGGA GGATTATGAG
    ACATCAGGTG TCGCAACCAG AAGTGCCTCA CAGGACATAA GGGAAGAAGA GTCAACCCCA
    GATACAGAGG ACTATTTCAG TAAAGACTCT ACTCCCAGTG ATAATTCATC CTCTCCTTTG
    ACCGATGACT CCAGACTTGA TGATGATGTT TTCCCATCTC CCAATAAACT CCGCACAACT
    GAAGATCTTT TTGCTATGAT ACACAGATCT AAAAGGAAAG TGCTGGGACG CAAAGATTCA
    GGGGAAGTCA GTGGAAAAGG TCGGCCTGTT GTACCACCTG TGACCACCCC TGTAAGCAAT
    CCTACTGTAT GCCCGGTCAT CCCTGCTCCC CCAGTTCCTT CAAACAACTC CCAGCGAGCC
    TCAGGACCCA TCTACAGGAG TGCCAAAAAG TCTAGTACAT CCAGTGAGGA GTTCAAACTC
    CTTCTGCTTA AGAAGGGTAG TCGCACAGAC TCAAGTTATC GCATGTCTGC TACAGAGATC
    CTTAAGAGCC CCATCACACC CAAGACTCAA GCAGAGCTGC TGTTAGAGGC CATGAGGCAA
    CCAGAGGAGA TCCCCTTACC CCAACTGGAT TCCACCAGCA GTGGAGATCC ACTTCCAAGT
    CCATTCCCTA AGGCCAACAG TGAGGGATTC TCCCCAAAAA CACTCACCAT GTCAGCTGCC
    TCCAGACAAG GACGTTCTAG AATTCCACCT GCAGCGAACA GCAGTCGCTA TAGCACGCGG
    AGTCGGCTGT ACACTGCCCC AATGCAGGCC ATATCAGAAG GAGAGACTGA GAACTCGGAT
    GGAAGTCCAC ACGATGACCG CTCCTCCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_009304395.1
    CDS320..5149
    Translation

    Target ORF information:

    RefSeq Version XM_009306120.3
    Organism Danio rerio(zebrafish)
    Definition Danio rerio Nance-Horan syndrome b (congenital cataracts and dental anomalies) (nhsb), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_009306120.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    ATGCCTTTTG CCAAACGGAT CGTTGAGCCC CAGCTGCTGG TTAGACATCC CATCCCTAAT 
    GATGAAGGGT TACTGTTCGA GGATCTGTGC TCCATCACCA ATGTGGCTCT ATCCCGGACC
    CTGCGGCAGC TCTCCGACCT GTCCAAACAC GCTTGCTCCT TATTTCAGGA GCTTGAGAAC
    GAAATAGTGT CCACAAACCA ACGGGTTTGG GCTTTGCAGA ACAAAATAGG CAAAATTCAG
    CAAACCGCCA GTGCGCTGGA TCCAAAAATG GAGGCTGTGC GTTTCAGGAG CATCTCTATA
    GCGGTCTCGA ATCTGGACAT TGAGAGTAAA CTGACGTCGC ACTATCAGGC TCCATGGCAT
    CAGCAACACA ATCTGTTCCA CACTTGCACA CGGCCAGCGT GTCTGGAGGA GCTGCACCGA
    CATGCCAAGC TCAACCTGCG GGCGCTGCAC AGAGACCAGC AGCAGCGTCA GCGTTCAACC
    AGCAGAGAGC GCAGTCGGGT CACTATATCA ATATCTGTGG CACCGCCAAT GCCAACCTTC
    CCTTCAACTC ACAAACTCAG ACGGCGAGAG CAGAGAGGTC GACATTCACG CATGGAGAGG
    AGCGTTAGAG AGAGTGATGT GCAAACTATC CAGAGGAAGG AGAGACCAGC CAAGGATTCT
    GAATTCAAAC CTGTCCTCAG AAAGGCTGGA ACCAACACAG AAACGGATGG AGAACTACAA
    GTGATGAGCC ATAGGCCTAA ATGTCCTGTT CCCAATGCAC CTACCACCCT GGACAAACAG
    ACTAACTGGT CTAAAGCCCT GCCTCTTCCC ACTCCAGAAG AAAGAATAAA GAATGACTCT
    CAAGTGATTT CATCCTGTAT CATTCCAATA AATGTTTCGG GTGTTGGATT TGACAGAGAA
    GCCAGTGCTC GCTGCTCTCT TGTTCACTCG CAATCGGTTC TACAGAGACG GCGGAAACTC
    AGGAGGCGAA AAACGATCAC TGGGATCCCC AAACGAGTTC AACAAGACAT GGATTCTGAC
    GAATCCCCTG TTGCTAGAGA AAGAACAGTG ATAATCCACG CCAATCCACA CAAGTCCCAC
    GAGTGGCATG AGGAGCTCTC CTTGAGTGGC AGAGTATTGC ATACTAAGGA TTCGGGCTGT
    CAGACAGATG ACTTCTTGAT TACTGCTCCA TCCCGAAGAC GCATCCGTGC CCAAAGAGGT
    CAGGGAATTC CAGCGTCTCT CTCTCACTCC ACTGGAAACA TTACCTCCCT CCCGGATCGT
    TCTGATACGG TTTATGCTGC AGCATCAGCC ACTCGTGTCC GTTCTAGGAG CCTTCCACGT
    GAGGGTGGTC GGCTTCTGGA TGAGGACCAA GATGACAGTG ATGATGATGA TGATGAAGAT
    GATGATGAAG ATGATGAAGA GGAAGATGAG GAGCTCTCTC CATATGAAGC CGAAGACTTC
    CTGCCAGGTC CCGGACAGAG AATTCCAAAG GATGAGGAAG AGAGTACTGA CGACCAAGCC
    ATGCCAGAGC TACAGTTTGG AAGTCTTAAG CGTATGCAGC ATTCAGAGAG CCCTGACCAC
    ACATGGATTG AGAGAGGCAG ATCCAGACTC CCACGGAAAG TAGACATGGG GAGCTGTGAG
    ATTTCCTCTA GTTCAGATAC TTTCAGCAGC CCCATACACT CTGCATCCAC TACAGGAGTG
    CTTGGCAGCC AGATTGACCA TAAAGAGGAT CACCAGTCCT CAAGTGGCAA CTGGAGTGGA
    AGCAGCTCCA CTTGCCCATC GCAGACATCC GAGACTCTTC CACCTGCTGC CTCCCCTCCC
    TTGACGGGAT CCTCACACTG TGATTCAGAA CTTTCCCTGA ATACTGGGTC CCATGTCATA
    GATGACAACA CTGGTTTCAT AATTGATCCC TATCACATAG ACAAGCCACA GGGACAGGGG
    CAACGATCCA ATTCATTTAC CTCATCAGCT ACTGATCAGA TGGATGATGC AGGGGTCAGC
    ACTGCTAGTG ATGGGGAGTG GAACTGTGCC CAGGACCAGC AGGATCAGTC CTGCACCCAA
    GACTTCAGCC CAAAGCGTTC CAGAGAGGAT GAGAGTGGTG TCAGCTGCCC TAGTTATTCT
    AGTGGGGAAA CTTACGAGAG CTTCAGTGAC CAAGCATCCG CTAGAAAATC TGAGATGCAC
    TACATTGTCG ACACTGAAGG ATTCTATTCC TCCATGCACT TTGACTCTGG TCTTAAGGGT
    AGCAGAAGCT ACTACAACTA TGCAGCCATT GACCCCGACT GTGGCCCAAG TGGTAATATA
    GCACCTGGCA TGACTGAATA CCCTCAGCCA GAGTACAGAG CCACCAGGAC ACTGGGCAGA
    CCCTGTCTCT CCTTAAGAAA ACCAAAGGCC AAGCCACCCC CACCAAAACG TAGCTCTTCA
    TTAAAGGAAA CAAGCAGTGA TGTGAACGTT CCAGAGCAGA ACGAACCAAA GATTACTTGT
    GAGTTGCCAC TGCCCTTGTC TTCCAGGGAG ATGAAACTGC AGCTGGACTT GGCTGATTCT
    GCAGGGCACC TTGAGACTGC AGGTCTTGAA TCACTTGGTG CATGGGGAGT GGAAAATGTT
    AGGGACATGC TTGACTGCTC CTCCCCATTC AGTTCCTCAG ACACACATTC CTTCAAAGAT
    GAAGGTGCTG TGCAAGCAGA CTATGCAGAC CTCTGGCTTC ACAATGACTT GAAATCAAAT
    GATCCTTATC GGTCCCTCTC TAACTCTAGC TCTGCTACAG GTACAACTGT TATTGAATGC
    ATCAAGTCAC CAGAAAGATC AGAAACGCAC ACCTGTCAAC CCAGGTCCAG ACCTTCTTCC
    CCAACTCTTC CTCCACCTGA AAGTGAATTT AAGCTTGCTT CTCCCGAAAA GCTGGCAGGC
    TTGGCGTCCC CTTCCAGTGG ATATTCCAGT CAGTCTGAAA CACCTACATC TTCCTTCCCC
    TCTGCTTTCT TCCCAGGGCC CCTGTCTCCA ACCAGTGGGA AGAGGAAGCC CAAAGTCCCT
    GAAAGGAAGT CCTCACTTTG TTCCTTACAG CAGCAACAGC TTTCAGTCAG AGACCCAGGC
    ATTTCCTGTA GGAGAGAAAC TGACTTCTAT GCCATACCCC CAAGTCACCT TGACCTAAGT
    GCTCTTCACA GTAAGCACTT TCCTCACAGA AATCAAATGC ACATCCTCCA CCAGAATAAG
    CAGAAAGCTG CCATAGCAGC AGCTGCTGCA GCAGAAGCTC GCAGTGCAGC AGCTGCTGCA
    GCAGAAGCTC GCAGTGCAGC AGCTGCTGCA GCAGAAGCTC GCAGTGCAGC AACTGCAGCC
    GCTGCTGCCG AGGCCTGCAC TGCAGCTACA GCTTCTACTA AAGAGAGTGC AACAAATACA
    GCACCCATCT CAGCCCATTT GGCTATTACT CCAACGGTTC TTAGATCAGT GCAACTGCGA
    TCTATATGTA GACCATCTGA TGGCAACCAA GGGCTTGATC AAGACAGTTC AAATCTCATA
    ACTCGTCCTA AGTGTCCCAC AATAATAACT GATGCCCCAT CATCTAGCAA CAGGCACAAC
    AGGAAGCCAC CAGCCTACAA ACCCCCTGCT GCACAGGTTT GTGATTCACA GATTCCACTG
    GTGGAAAACA TTGTTTTTCC ACAAGAAGAA GTGAGAGTGA GACAGGAGAG GTTTGGTCCT
    GGTACTTACT GGGCAATGAC TGCTTTCAGA ACTCCTGTGG ACCCTAATTG TGAAAAAGAA
    GTCTCTTTAA AAAGGTCATC TCAGTGTTTT CAGCAAGAAC AAGACAGCAG AAGGTGCCTA
    GATGTGCAAA AGACATTGTC ACCAGAGAGA CTAAAACAAG ATTTGAATCA ACTATCGCGG
    CCAGACCCTT CAACACAGTC CATGTGTTTG GCCAGCACAA TGCCACCCCC TGCTTATAGT
    GAGATACAGA GGAGCAATTT TGTACTGTGC AACAGTCCTG ACAAAGTGCC TGTTTCAACT
    CAAGAGGCAT CGCATTCTTA CACAATCAGC GATGTACAAG GCAGAGAGGA GGATTATGAG
    ACATCAGGTG TCGCAACCAG AAGTGCCTCA CAGGACATAA GGGAAGAAGA GTCAACCCCA
    GATACAGAGG ACTATTTCAG TAAAGACTCT ACTCCCAGTG ATAATTCATC CTCTCCTTTG
    ACCGATGACT CCAGACTTGA TGATGATGTT TTCCCATCTC CCAATAAACT CCGCACAACT
    GAAGATCTTT TTGCTATGAT ACACAGATCT AAAAGGAAAG TGCTGGGACG CAAAGATTCA
    GGGGAAGTCA GTGGAAAAGG TCGGCCTGTT GTACCACCTG TGACCACCCC TGTAAGCAAT
    CCTACTGTAT GCCCGGTCAT CCCTGCTCCC CCAGTTCCTT CAAACAACTC CCAGCGAGCC
    TCAGGACCCA TCTACAGGAG TGCCAAAAAG TCTAGTACAT CCAGTGAGGA GTTCAAACTC
    CTTCTGCTTA AGAAGGGTAG TCGCACAGAC TCAAGTTATC GCATGTCTGC TACAGAGATC
    CTTAAGAGCC CCATCACACC CAAGACTCAA GCAGAGCTGC TGTTAGAGGC CATGAGGCAA
    CCAGAGGAGA TCCCCTTACC CCAACTGGAT TCCACCAGCA GTGGAGATCC ACTTCCAAGT
    CCATTCCCTA AGGCCAACAG TGAGGGATTC TCCCCAAAAA CACTCACCAT GTCAGCTGCC
    TCCAGACAAG GACGTTCTAG AATTCCACCT GCAGCGAACA GCAGTCGCTA TAGCACGCGG
    AGTCGGCTGT ACACTGCCCC AATGCAGGCC ATATCAGAAG GAGAGACTGA GAACTCGGAT
    GGAAGTCCAC ACGATGACCG CTCCTCCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

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