ccdc28b cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Gene Symbol	ccdc28b
Entrez Gene ID	559814
Full Name	coiled-coil domain containing 28B
Synonyms	si:dkey-71p21.10
General protein information	Preferred Names coiled-coil domain containing 28B Names coiled-coil domain-containing protein 28B
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA Protein Name

NM_001145573.1 NP_001139045.1 coiled-coil domain-containing protein 28B
Pathways from BioSystems

mRNA	Protein	Name
NM_001145573.1	NP_001139045.1	coiled-coil domain-containing protein 28B

Homologies

Canis lupus familiaris (dog)	CCDC28B	XP_544438.4
Homo sapiens (human)	CCDC28B	NP_077272.2
	CCDC28B	XP_001102480.2
Gallus gallus (chicken)	CCDC28B	XP_004947881.1
Pan troglodytes (chimpanzee)	CCDC28B	XP_513278.1
Mus musculus (house mouse)	Ccdc28b	NP_079731.2
Rattus norvegicus (Norway rat)	Ccdc28b	NP_001128161.1
Danio rerio (zebrafish)	ccdc28b	NP_001139045.1
Xenopus tropicalis (tropical clawed frog)	ccdc28b	NP_001016093.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
Pasquier J, Cabau C, Nguyen T, Jouanno E, Severac D, Braasch I, Journot L, Pontarotti P, Klopp C, Postlethwait JH, Guiguen Y, Bobe J
BMC genomics17368(2016 05)

RFX transcription factors are essential for hearing in mice.
Elkon R, Milon B, Morrison L, Shah M, Vijayakumar S, Racherla M, Leitch CC, Silipino L, Hadi S, Weiss-Gayet M, Barras E, Schmid CD, Ait-Lounis A, Barnes A, Song Y, Eisenman DJ, Eliyahu E, Frolenkov GI, Strome SE, Durand B, Zaghloul NA, Jones SM, Reith W, Hertzano R
Nature communications68549(2015 Oct)

The Bardet-Biedl syndrome-related protein CCDC28B modulates mTORC2 function and interacts with SIN1 to control cilia length independently of the mTOR complex.
Cardenas-Rodriguez M, Irigo?n F, Osborn DP, Gascue C, Katsanis N, Beales PL, Badano JL
Human molecular genetics22(20)4031-42(2013 Oct)

Characterization of CCDC28B reveals its role in ciliogenesis and provides insight to understand its modifier effect on Bardet-Biedl syndrome.
Cardenas-Rodriguez M, Osborn DP, Irigo?n F, Gra?a M, Romero H, Beales PL, Badano JL
Human genetics132(1)91-105(2013 Jan)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Findings implicate CCDC28B in the regulation of mTORC2, and uncover a novel function of SIN1 regulating cilia length that is likely independent of mTOR signaling.
Title: The Bardet-Biedl syndrome-related protein CCDC28B modulates mTORC2 function and interacts with SIN1 to control cilia length independently of the mTOR complex.

Depletion of Ccdc28b results in defective ciliogenesis and causes phenotypes that are characteristic of Bardet-Biedl syndrome and other ciliopathy mutants including hydrocephalus, left-right axis determination defects and renal function impairment.
Title: Characterization of CCDC28B reveals its role in ciliogenesis and provides insight to understand its modifier effect on Bardet-Biedl syndrome.

The following ccdc28b gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ccdc28b cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ODa01465	NM_001145573.1 Latest version!	Danio rerio coiled-coil domain containing 28B (ccdc28b), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$174.30 $249.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

ccdc28b ( NM_001145573.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_001139045.1 Danio rerio coiled-coil domain containing 28B (ccdc28b), mRNA.

Price & Availability↑

CloneID	ODa01465
Clone ID Related Accession (Same CDS sequence)	NM_001145573.1
Accession Version	NM_001145573.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 642bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-11-23
Organism	Danio rerio(zebrafish)
Product	coiled-coil domain-containing protein 28B
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BX284673.9. On Mar 5, 2009 this sequence version replaced XM_683187.3. ##Evidence-Data-START## Transcript exon combination :: GFIL01003237.1, GDQH01008469.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2168446, SAMEA2168447 [ECO:0000348] ##Evidence-Data-END##

ATGGAGGACA AGAGGAAGAA AAGAAGTCCC AAAGTGTCTC TTCCCCAGCC CCCTCCTCCA 
CCAATCAACC CTCGAAAGCT CACCGTCCTC CCCGCGAGCA AGAGCGCCAC ATTCTCTCTG 
GGCCTACCCC AGCCTCCCTC CCCCAAACCC AGGGGCAAAT ACAAGAGATC GGTGGGGGCA 
ATGGGTCCAT CCAAAGAAGC TCTTGCAGTT CCTGTGGTTC CCCCTAAAAA CACAAGGCCT 
CATAGAGAGA AGCCCAGAGC TCCTCAGCCT GCTGGTCCAA GCCGAGTGTC CCAGTCGTCC 
TCACCGCTCC AGCACACATT TCTCACCGAC GTCTCCGATG TGAGAGAGAT GGAGGGAGGA 
CTGCTCAACC TGCTCAATGA CTTTCACTCA GGAAAACTGC AGGCATTTGG TAAAGTGTGT 
TCTTTCGAGC AACTAGAGCA TGTGAGGGAG ATGCAGGAAC GTCTGGCTCG GTTGCATTTC 
AGTCTGGACA GTCATGTGGA AGAGCTGTCT GAAGACCAGA GGAAAAACGC TTCAGATCGC 
AATCTAGAAC ACCTGCTTTC TAACCTGGAA GAGTTAAGCA CCTCAATACA GAAACTACAT 
CTGGCAGAAA ATCAAGACTT ACCCAAGACC TCCAACACCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001139045.1
CDS	186..827
Translation	MEDKRKKRSPKVSLPQPPPPPINPRKLTVLPASKSATFSLGLPQPPSPKPRGKYKRSVGAMGPSKEALAVPVVPPKNTRPHREKPRAPQPAGPSRVSQSSSPLQHTFLTDVSDVREMEGGLLNLLNDFHSGKLQAFGKVCSFEQLEHVREMQERLARLHFSLDSHVEELSEDQRKNASDRNLEHLLSNLEELSTSIQKLHLAENQDLPKTSNT

Target ORF information:

RefSeq Version	NM_001145573.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio coiled-coil domain containing 28B (ccdc28b), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001145573.1

ORF Insert Sequence:

ATGGAGGACA AGAGGAAGAA AAGAAGTCCC AAAGTGTCTC TTCCCCAGCC CCCTCCTCCA 
CCAATCAACC CTCGAAAGCT CACCGTCCTC CCCGCGAGCA AGAGCGCCAC ATTCTCTCTG 
GGCCTACCCC AGCCTCCCTC CCCCAAACCC AGGGGCAAAT ACAAGAGATC GGTGGGGGCA 
ATGGGTCCAT CCAAAGAAGC TCTTGCAGTT CCTGTGGTTC CCCCTAAAAA CACAAGGCCT 
CATAGAGAGA AGCCCAGAGC TCCTCAGCCT GCTGGTCCAA GCCGAGTGTC CCAGTCGTCC 
TCACCGCTCC AGCACACATT TCTCACCGAC GTCTCCGATG TGAGAGAGAT GGAGGGAGGA 
CTGCTCAACC TGCTCAATGA CTTTCACTCA GGAAAACTGC AGGCATTTGG TAAAGTGTGT 
TCTTTCGAGC AACTAGAGCA TGTGAGGGAG ATGCAGGAAC GTCTGGCTCG GTTGCATTTC 
AGTCTGGACA GTCATGTGGA AGAGCTGTCT GAAGACCAGA GGAAAAACGC TTCAGATCGC 
AATCTAGAAC ACCTGCTTTC TAACCTGGAA GAGTTAAGCA CCTCAATACA GAAACTACAT 
CTGGCAGAAA ATCAAGACTT ACCCAAGACC TCCAACACCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
BMC genomics17368(2016 05)
Pasquier J,Cabau C,Nguyen T,Jouanno E,Severac D,Braasch I,Journot L,Pontarotti P,Klopp C,Postlethwait JH,Guiguen Y,Bobe J

RFX transcription factors are essential for hearing in mice.
Nature communications68549(2015 Oct)
Elkon R,Milon B,Morrison L,Shah M,Vijayakumar S,Racherla M,Leitch CC,Silipino L,Hadi S,Weiss-Gayet M,Barras E,Schmid CD,Ait-Lounis A,Barnes A,Song Y,Eisenman DJ,Eliyahu E,Frolenkov GI,Strome SE,Durand B,Zaghloul NA,Jones SM,Reith W,Hertzano R

The Bardet-Biedl syndrome-related protein CCDC28B modulates mTORC2 function and interacts with SIN1 to control cilia length independently of the mTOR complex.
Human molecular genetics22(20)4031-42(2013 Oct)
Cardenas-Rodriguez M,Irigo?n F,Osborn DP,Gascue C,Katsanis N,Beales PL,Badano JL

Characterization of CCDC28B reveals its role in ciliogenesis and provides insight to understand its modifier effect on Bardet-Biedl syndrome.
Human genetics132(1)91-105(2013 Jan)
Cardenas-Rodriguez M,Osborn DP,Irigo?n F,Gra?a M,Romero H,Beales PL,Badano JL

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ccdc28b cDNA ORF clone, Danio rerio(zebrafish)

Gene

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Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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