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best1 cDNA ORF clone, Danio rerio(zebrafish)

Summary

Gene Symbol best1
Entrez Gene ID 560608
Full Name bestrophin 1
Synonyms si:ch1073-296i8.4
General protein information
Preferred Names

bestrophin 1

Names

bestrophin-1
Gene Type protein-coding
Organism Danio rerio(zebrafish)

Reference Sequences (RefSeq)

mRNA and Protein(s)

mRNA Protein Name
XM_684006.8 XP_689098.3 bestrophin-1 isoform X1
XM_009297704.2 XP_009295979.1 bestrophin-1 isoform X2
XM_684006.9 XP_689098.3 bestrophin-1 isoform X1
XM_009297704.3 XP_009295979.1 bestrophin-1 isoform X2

Pathways from BioSystems


Homologies


Homo sapiens (human) BEST1 NP_004174.1
Bos taurus (cattle) BEST1 NP_001073714.1
Mus musculus (house mouse) Best1 NP_036043.2
Danio rerio (zebrafish) LOC560608 XP_689098.3
Pan troglodytes (chimpanzee) BEST1 XP_001151529.1
LOC718993 XP_001116591.1
Xenopus tropicalis (tropical clawed frog) best1 NP_001155223.1
Canis lupus familiaris (dog) BEST1 NP_001091014.1
Rattus norvegicus (Norway rat) Best1 NP_001011940.1
Gallus gallus (chicken) BEST1 XP_004941545.1

Gene Ontology

The following best1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the best1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
ODa43317 XM_009297704.3
Latest version!		 Danio rerio bestrophin 1 (best1), transcript variant X2, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $258.30
$369.00
ODa29027 XM_684006.9
Latest version!		 Danio rerio bestrophin 1 (best1), transcript variant X1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 12-14 $426.30
$609.00
View Old Accession Versions >>
ODa29027 XM_684006.8 Danio rerio bestrophin 1 (best1), transcript variant X1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 12-14 $426.30
$609.00
ODa43317 XM_009297704.2 Danio rerio bestrophin 1 (best1), transcript variant X2, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $258.30
$369.00
Hide Old Accession Versions >>

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)


CloneID ODa43317
Clone ID Related Accession (Same CDS sequence) XM_009297704.2 , XM_009297704.3
Accession Version XM_009297704.2 Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 1365bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2016-06-23
Organism Danio rerio(Zebrafish)
Product bestrophin-1 isoform X2
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007136.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009297704.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
ATGATTGGAT CAGCTTACCT CTCGTTTATA CACAGTGTCT TCTGCGACAA TCAGTACTTG 
GTGGTAACCG TGGCTGTGTA CAGTTTCTTT TTGACTTGTC TGATTGGTCG GCAGTTTCTT 
GACCCCGCCC AGGGTTACCC GGGTCACAAC CTCGACTTCT ACCTGCCTGT CTTCACTTTA 
CTCCAGTTCT TCTTCTACGT GGGCTGGCTT AAGGTTGCAG AGCAGCTTAT TAATCCATTT 
GGGGAGGATG ATGACGACTT TGAGACTAAC TGGCTGGTGG ACCGTAATTT ACAGGTGTCT 
CTGCTGTCAG TGGATGAGAT GTATGATCTG GTTCCTCTGG TTGAGAGAGA TAAATACTGG 
GATGAAGCTG AGCCTCAGCC GCCGTACACC GCCGCCAGCG CAGAACACCG CAAACCCTCA 
TTCATGGGAT CTGCGCTGGA CATCAGCGTT CCTAAAGAGG AAATGGAATT CCAGCACAAT 
CTGGAGCAGA TTAAAGAGCA CGAGGAGGCC AATCACTCCA CTCCATTCCT GGGCAGCTTG 
AGTCGACTTC TGGGCGTTCA GTCGCCCAAT TTCCCCCGCT CGACCCCTAC ATCAAGAGTC 
TCCCTCCTGC GACGCCGACC CCGTGCTCCG TTCTCTCGTT TCCCACTTTA CCTCCATTCT 
GAGAGCTCAC AGATGCCCAG CCACAGTCGC AACACGGATG TGAGCGAGGA CGCCTTTTCA 
GCAATGCCTC TGTATGAACG ACCCGGTTTT TACAGCTGCC CCCAAACGCC CATCCACTGC 
ATCCCTCCAC CCGTTCCACG ACCAAGACCA CGGAGAGCGC ATGGTGACAG CTCCAGCTCC 
TTAACACAAC CTATGGTGGG CTCACAAATC CTGGCTCTGC CTGACACACC CGCACCTCCT 
TCATCCGCCT TCCCTTGGGT GGGGGAAGAC AGCGAACATC CGGCGTTCTC CTTTCCAGAT 
CCTGTCCCTG AAATATGTCC GTTAACCAAA GCACGACTAA TTCAAGGCTT ACCATCGCGC 
CGTCCTCTCC CTCTGCGACT ATCTGTCGAT AAATCTCCAT CTCCATCTAC ACCAGAGAGG 
GTGTTTTCAT TCACACCGCC ATCTTGGCCG CAAACGCAAA TCAACACCGC TAGTTCAGTG 
AATGTGAACG TGACAAATAG TAATAATAAT CTGTGCAACG GCACAGCAAA CAACCCCTGG 
CCAATCAGCA GAAGCAGCAA TGCAAGCACA GCCAATCCTG TCAGCACGAG CCCTACGGCG 
ACTCAGCACT CAGCCAATCA GCAGAACTCT CCCAACGATT CTGGAATCTC ATTGGCTGAC 
GGGGATCTGT TGGGCGCAGC TGTGAAAACA AGGGAACAGC TCTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_009295979.1
CDS135..1499
Translation

Target ORF information:

RefSeq Version XM_009297704.2
Organism Danio rerio(Zebrafish)
Definition Danio rerio bestrophin 1 (best1), transcript variant X2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 XM_009297704.2

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
ATGATTGGAT CAGCTTACCT CTCGTTTATA CACAGTGTCT TCTGCGACAA TCAGTACTTG 
GTGGTAACCG TGGCTGTGTA CAGTTTCTTT TTGACTTGTC TGATTGGTCG GCAGTTTCTT 
GACCCCGCCC AGGGTTACCC GGGTCACAAC CTCGACTTCT ACCTGCCTGT CTTCACTTTA 
CTCCAGTTCT TCTTCTACGT GGGCTGGCTT AAGGTTGCAG AGCAGCTTAT TAATCCATTT 
GGGGAGGATG ATGACGACTT TGAGACTAAC TGGCTGGTGG ACCGTAATTT ACAGGTGTCT 
CTGCTGTCAG TGGATGAGAT GTATGATCTG GTTCCTCTGG TTGAGAGAGA TAAATACTGG 
GATGAAGCTG AGCCTCAGCC GCCGTACACC GCCGCCAGCG CAGAACACCG CAAACCCTCA 
TTCATGGGAT CTGCGCTGGA CATCAGCGTT CCTAAAGAGG AAATGGAATT CCAGCACAAT 
CTGGAGCAGA TTAAAGAGCA CGAGGAGGCC AATCACTCCA CTCCATTCCT GGGCAGCTTG 
AGTCGACTTC TGGGCGTTCA GTCGCCCAAT TTCCCCCGCT CGACCCCTAC ATCAAGAGTC 
TCCCTCCTGC GACGCCGACC CCGTGCTCCG TTCTCTCGTT TCCCACTTTA CCTCCATTCT 
GAGAGCTCAC AGATGCCCAG CCACAGTCGC AACACGGATG TGAGCGAGGA CGCCTTTTCA 
GCAATGCCTC TGTATGAACG ACCCGGTTTT TACAGCTGCC CCCAAACGCC CATCCACTGC 
ATCCCTCCAC CCGTTCCACG ACCAAGACCA CGGAGAGCGC ATGGTGACAG CTCCAGCTCC 
TTAACACAAC CTATGGTGGG CTCACAAATC CTGGCTCTGC CTGACACACC CGCACCTCCT 
TCATCCGCCT TCCCTTGGGT GGGGGAAGAC AGCGAACATC CGGCGTTCTC CTTTCCAGAT 
CCTGTCCCTG AAATATGTCC GTTAACCAAA GCACGACTAA TTCAAGGCTT ACCATCGCGC 
CGTCCTCTCC CTCTGCGACT ATCTGTCGAT AAATCTCCAT CTCCATCTAC ACCAGAGAGG 
GTGTTTTCAT TCACACCGCC ATCTTGGCCG CAAACGCAAA TCAACACCGC TAGTTCAGTG 
AATGTGAACG TGACAAATAG TAATAATAAT CTGTGCAACG GCACAGCAAA CAACCCCTGG 
CCAATCAGCA GAAGCAGCAA TGCAAGCACA GCCAATCCTG TCAGCACGAG CCCTACGGCG 
ACTCAGCACT CAGCCAATCA GCAGAACTCT CCCAACGATT CTGGAATCTC ATTGGCTGAC 
GGGGATCTGT TGGGCGCAGC TGTGAAAACA AGGGAACAGC TCTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID ODa43317
Clone ID Related Accession (Same CDS sequence) XM_009297704.2 , XM_009297704.3
Accession Version XM_009297704.3 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 1365bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2017-06-14
Organism Danio rerio(zebrafish)
Product bestrophin-1 isoform X2
Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007136.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009297704.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
ATGATTGGAT CAGCTTACCT CTCGTTTATA CACAGTGTCT TCTGCGACAA TCAGTACTTG 
GTGGTAACCG TGGCTGTGTA CAGTTTCTTT TTGACTTGTC TGATTGGTCG GCAGTTTCTT 
GACCCCGCCC AGGGTTACCC GGGTCACAAC CTCGACTTCT ACCTGCCTGT CTTCACTTTA 
CTCCAGTTCT TCTTCTACGT GGGCTGGCTT AAGGTTGCAG AGCAGCTTAT TAATCCATTT 
GGGGAGGATG ATGACGACTT TGAGACTAAC TGGCTGGTGG ACCGTAATTT ACAGGTGTCT 
CTGCTGTCAG TGGATGAGAT GTATGATCTG GTTCCTCTGG TTGAGAGAGA TAAATACTGG 
GATGAAGCTG AGCCTCAGCC GCCGTACACC GCCGCCAGCG CAGAACACCG CAAACCCTCA 
TTCATGGGAT CTGCGCTGGA CATCAGCGTT CCTAAAGAGG AAATGGAATT CCAGCACAAT 
CTGGAGCAGA TTAAAGAGCA CGAGGAGGCC AATCACTCCA CTCCATTCCT GGGCAGCTTG 
AGTCGACTTC TGGGCGTTCA GTCGCCCAAT TTCCCCCGCT CGACCCCTAC ATCAAGAGTC 
TCCCTCCTGC GACGCCGACC CCGTGCTCCG TTCTCTCGTT TCCCACTTTA CCTCCATTCT 
GAGAGCTCAC AGATGCCCAG CCACAGTCGC AACACGGATG TGAGCGAGGA CGCCTTTTCA 
GCAATGCCTC TGTATGAACG ACCCGGTTTT TACAGCTGCC CCCAAACGCC CATCCACTGC 
ATCCCTCCAC CCGTTCCACG ACCAAGACCA CGGAGAGCGC ATGGTGACAG CTCCAGCTCC 
TTAACACAAC CTATGGTGGG CTCACAAATC CTGGCTCTGC CTGACACACC CGCACCTCCT 
TCATCCGCCT TCCCTTGGGT GGGGGAAGAC AGCGAACATC CGGCGTTCTC CTTTCCAGAT 
CCTGTCCCTG AAATATGTCC GTTAACCAAA GCACGACTAA TTCAAGGCTT ACCATCGCGC 
CGTCCTCTCC CTCTGCGACT ATCTGTCGAT AAATCTCCAT CTCCATCTAC ACCAGAGAGG 
GTGTTTTCAT TCACACCGCC ATCTTGGCCG CAAACGCAAA TCAACACCGC TAGTTCAGTG 
AATGTGAACG TGACAAATAG TAATAATAAT CTGTGCAACG GCACAGCAAA CAACCCCTGG 
CCAATCAGCA GAAGCAGCAA TGCAAGCACA GCCAATCCTG TCAGCACGAG CCCTACGGCG 
ACTCAGCACT CAGCCAATCA GCAGAACTCT CCCAACGATT CTGGAATCTC ATTGGCTGAC 
GGGGATCTGT TGGGCGCAGC TGTGAAAACA AGGGAACAGC TCTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_009295979.1
CDS135..1499
Translation

Target ORF information:

RefSeq Version XM_009297704.3
Organism Danio rerio(zebrafish)
Definition Danio rerio bestrophin 1 (best1), transcript variant X2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 XM_009297704.3

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
ATGATTGGAT CAGCTTACCT CTCGTTTATA CACAGTGTCT TCTGCGACAA TCAGTACTTG 
GTGGTAACCG TGGCTGTGTA CAGTTTCTTT TTGACTTGTC TGATTGGTCG GCAGTTTCTT 
GACCCCGCCC AGGGTTACCC GGGTCACAAC CTCGACTTCT ACCTGCCTGT CTTCACTTTA 
CTCCAGTTCT TCTTCTACGT GGGCTGGCTT AAGGTTGCAG AGCAGCTTAT TAATCCATTT 
GGGGAGGATG ATGACGACTT TGAGACTAAC TGGCTGGTGG ACCGTAATTT ACAGGTGTCT 
CTGCTGTCAG TGGATGAGAT GTATGATCTG GTTCCTCTGG TTGAGAGAGA TAAATACTGG 
GATGAAGCTG AGCCTCAGCC GCCGTACACC GCCGCCAGCG CAGAACACCG CAAACCCTCA 
TTCATGGGAT CTGCGCTGGA CATCAGCGTT CCTAAAGAGG AAATGGAATT CCAGCACAAT 
CTGGAGCAGA TTAAAGAGCA CGAGGAGGCC AATCACTCCA CTCCATTCCT GGGCAGCTTG 
AGTCGACTTC TGGGCGTTCA GTCGCCCAAT TTCCCCCGCT CGACCCCTAC ATCAAGAGTC 
TCCCTCCTGC GACGCCGACC CCGTGCTCCG TTCTCTCGTT TCCCACTTTA CCTCCATTCT 
GAGAGCTCAC AGATGCCCAG CCACAGTCGC AACACGGATG TGAGCGAGGA CGCCTTTTCA 
GCAATGCCTC TGTATGAACG ACCCGGTTTT TACAGCTGCC CCCAAACGCC CATCCACTGC 
ATCCCTCCAC CCGTTCCACG ACCAAGACCA CGGAGAGCGC ATGGTGACAG CTCCAGCTCC 
TTAACACAAC CTATGGTGGG CTCACAAATC CTGGCTCTGC CTGACACACC CGCACCTCCT 
TCATCCGCCT TCCCTTGGGT GGGGGAAGAC AGCGAACATC CGGCGTTCTC CTTTCCAGAT 
CCTGTCCCTG AAATATGTCC GTTAACCAAA GCACGACTAA TTCAAGGCTT ACCATCGCGC 
CGTCCTCTCC CTCTGCGACT ATCTGTCGAT AAATCTCCAT CTCCATCTAC ACCAGAGAGG 
GTGTTTTCAT TCACACCGCC ATCTTGGCCG CAAACGCAAA TCAACACCGC TAGTTCAGTG 
AATGTGAACG TGACAAATAG TAATAATAAT CTGTGCAACG GCACAGCAAA CAACCCCTGG 
CCAATCAGCA GAAGCAGCAA TGCAAGCACA GCCAATCCTG TCAGCACGAG CCCTACGGCG 
ACTCAGCACT CAGCCAATCA GCAGAACTCT CCCAACGATT CTGGAATCTC ATTGGCTGAC 
GGGGATCTGT TGGGCGCAGC TGTGAAAACA AGGGAACAGC TCTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID ODa29027
Clone ID Related Accession (Same CDS sequence) XM_684006.8 , XM_684006.9
Accession Version XM_684006.8 Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 2019bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2016-06-23
Organism Danio rerio(Zebrafish)
Product bestrophin-1 isoform X1
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007136.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_684006.7. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
ATGACGGTGA CGTACTCGCG GCGGGTGGCA GATGCGCGTC TGGGCACCTT TTATCGCCTT 
CTTCTGCGAT GGAAAGGAAG CATTTATAAG CTGCTGTATC GCGAGCTGCT GATCTTCACT 
GTGATGTACT GCATTATCAG CATCACATAC AGGTGTGTAC TGACTGAAGA GCAGCGCAGG 
ATGTTTGAGA AGCTCTCCCT GTACTGTGAT CAGTACGCTC AGCTCATTCC TGTGTCATTC 
GTACTGGGTT TCTATGTGAC TCTGGTGGTG TCCCGCTGGT GGGGTCAGTT CGAGAGCGTC 
CCCTGGCCGG ACCGCCTGTC AGCTCTGGTC AGTGGACATG TACGGGGTGC AGATGAAGGA 
GCCAGACTCA TACGCAGGAG TTTAATGCGC TACGCAAACC TGTCCGGCAT CCTCATCTAC 
CGCTCCGTCA GCACGGCTGT CTACAAGAGA TTCCCGACCA TGAGCCACTT GGTTCAAGCA 
GGTCTGATGA CAGCGGAGGA GCTGAGGCAG CTGCAGGAGC TTCCTTCACC TCATAATAAG 
TTCTGGGTTC CCTGCATGTG GTTTGTCAGT CTGGCTATGA GAGCTCGATC TGAAGGACGA 
ATCAATAACG ACGTCGCAAT GACAGCCATC CTCAACGAAC TGAACACGCT TCGGTCTCAA 
TGTATGAGGC TGTACGGATA TGATTGGATC AGCTTACCTC TCGTTTATAC ACAGGTGGTA 
ACCGTGGCTG TGTACAGTTT CTTTTTGACT TGTCTGATTG GTCGGCAGTT TCTTGACCCC 
GCCCAGGGTT ACCCGGGTCA CAACCTCGAC TTCTACCTGC CTGTCTTCAC TTTACTCCAG 
TTCTTCTTCT ACGTGGGCTG GCTTAAGGTT GCAGAGCAGC TTATTAATCC ATTTGGGGAG 
GATGATGACG ACTTTGAGAC TAACTGGCTG GTGGACCGTA ATTTACAGGT GTCTCTGCTG 
TCAGTGGATG AGATGTATGA TCTGGTTCCT CTGGTTGAGA GAGATAAATA CTGGGATGAA 
GCTGAGCCTC AGCCGCCGTA CACCGCCGCC AGCGCAGAAC ACCGCAAACC CTCATTCATG 
GGATCTGCGC TGGACATCAG CGTTCCTAAA GAGGAAATGG AATTCCAGCA CAATCTGGAG 
CAGATTAAAG AGCACGAGGA GGCCAATCAC TCCACTCCAT TCCTGGGCAG CTTGAGTCGA 
CTTCTGGGCG TTCAGTCGCC CAATTTCCCC CGCTCGACCC CTACATCAAG AGTCTCCCTC 
CTGCGACGCC GACCCCGTGC TCCGTTCTCT CGTTTCCCAC TTTACCTCCA TTCTGAGAGC 
TCACAGATGC CCAGCCACAG TCGCAACACG GATGTGAGCG AGGACGCCTT TTCAGCAATG 
CCTCTGTATG AACGACCCGG TTTTTACAGC TGCCCCCAAA CGCCCATCCA CTGCATCCCT 
CCACCCGTTC CACGACCAAG ACCACGGAGA GCGCATGGTG ACAGCTCCAG CTCCTTAACA 
CAACCTATGG TGGGCTCACA AATCCTGGCT CTGCCTGACA CACCCGCACC TCCTTCATCC 
GCCTTCCCTT GGGTGGGGGA AGACAGCGAA CATCCGGCGT TCTCCTTTCC AGATCCTGTC 
CCTGAAATAT GTCCGTTAAC CAAAGCACGA CTAATTCAAG GCTTACCATC GCGCCGTCCT 
CTCCCTCTGC GACTATCTGT CGATAAATCT CCATCTCCAT CTACACCAGA GAGGGTGTTT 
TCATTCACAC CGCCATCTTG GCCGCAAACG CAAATCAACA CCGCTAGTTC AGTGAATGTG 
AACGTGACAA ATAGTAATAA TAATCTGTGC AACGGCACAG CAAACAACCC CTGGCCAATC 
AGCAGAAGCA GCAATGCAAG CACAGCCAAT CCTGTCAGCA CGAGCCCTAC GGCGACTCAG 
CACTCAGCCA ATCAGCAGAA CTCTCCCAAC GATTCTGGAA TCTCATTGGC TGACGGGGAT 
CTGTTGGGCG CAGCTGTGAA AACAAGGGAA CAGCTCTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_689098.3
CDS130..2148
Translation

Target ORF information:

RefSeq Version XM_684006.8
Organism Danio rerio(Zebrafish)
Definition Danio rerio bestrophin 1 (best1), transcript variant X1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 XM_684006.8

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
ATGACGGTGA CGTACTCGCG GCGGGTGGCA GATGCGCGTC TGGGCACCTT TTATCGCCTT 
CTTCTGCGAT GGAAAGGAAG CATTTATAAG CTGCTGTATC GCGAGCTGCT GATCTTCACT 
GTGATGTACT GCATTATCAG CATCACATAC AGGTGTGTAC TGACTGAAGA GCAGCGCAGG 
ATGTTTGAGA AGCTCTCCCT GTACTGTGAT CAGTACGCTC AGCTCATTCC TGTGTCATTC 
GTACTGGGTT TCTATGTGAC TCTGGTGGTG TCCCGCTGGT GGGGTCAGTT CGAGAGCGTC 
CCCTGGCCGG ACCGCCTGTC AGCTCTGGTC AGTGGACATG TACGGGGTGC AGATGAAGGA 
GCCAGACTCA TACGCAGGAG TTTAATGCGC TACGCAAACC TGTCCGGCAT CCTCATCTAC 
CGCTCCGTCA GCACGGCTGT CTACAAGAGA TTCCCGACCA TGAGCCACTT GGTTCAAGCA 
GGTCTGATGA CAGCGGAGGA GCTGAGGCAG CTGCAGGAGC TTCCTTCACC TCATAATAAG 
TTCTGGGTTC CCTGCATGTG GTTTGTCAGT CTGGCTATGA GAGCTCGATC TGAAGGACGA 
ATCAATAACG ACGTCGCAAT GACAGCCATC CTCAACGAAC TGAACACGCT TCGGTCTCAA 
TGTATGAGGC TGTACGGATA TGATTGGATC AGCTTACCTC TCGTTTATAC ACAGGTGGTA 
ACCGTGGCTG TGTACAGTTT CTTTTTGACT TGTCTGATTG GTCGGCAGTT TCTTGACCCC 
GCCCAGGGTT ACCCGGGTCA CAACCTCGAC TTCTACCTGC CTGTCTTCAC TTTACTCCAG 
TTCTTCTTCT ACGTGGGCTG GCTTAAGGTT GCAGAGCAGC TTATTAATCC ATTTGGGGAG 
GATGATGACG ACTTTGAGAC TAACTGGCTG GTGGACCGTA ATTTACAGGT GTCTCTGCTG 
TCAGTGGATG AGATGTATGA TCTGGTTCCT CTGGTTGAGA GAGATAAATA CTGGGATGAA 
GCTGAGCCTC AGCCGCCGTA CACCGCCGCC AGCGCAGAAC ACCGCAAACC CTCATTCATG 
GGATCTGCGC TGGACATCAG CGTTCCTAAA GAGGAAATGG AATTCCAGCA CAATCTGGAG 
CAGATTAAAG AGCACGAGGA GGCCAATCAC TCCACTCCAT TCCTGGGCAG CTTGAGTCGA 
CTTCTGGGCG TTCAGTCGCC CAATTTCCCC CGCTCGACCC CTACATCAAG AGTCTCCCTC 
CTGCGACGCC GACCCCGTGC TCCGTTCTCT CGTTTCCCAC TTTACCTCCA TTCTGAGAGC 
TCACAGATGC CCAGCCACAG TCGCAACACG GATGTGAGCG AGGACGCCTT TTCAGCAATG 
CCTCTGTATG AACGACCCGG TTTTTACAGC TGCCCCCAAA CGCCCATCCA CTGCATCCCT 
CCACCCGTTC CACGACCAAG ACCACGGAGA GCGCATGGTG ACAGCTCCAG CTCCTTAACA 
CAACCTATGG TGGGCTCACA AATCCTGGCT CTGCCTGACA CACCCGCACC TCCTTCATCC 
GCCTTCCCTT GGGTGGGGGA AGACAGCGAA CATCCGGCGT TCTCCTTTCC AGATCCTGTC 
CCTGAAATAT GTCCGTTAAC CAAAGCACGA CTAATTCAAG GCTTACCATC GCGCCGTCCT 
CTCCCTCTGC GACTATCTGT CGATAAATCT CCATCTCCAT CTACACCAGA GAGGGTGTTT 
TCATTCACAC CGCCATCTTG GCCGCAAACG CAAATCAACA CCGCTAGTTC AGTGAATGTG 
AACGTGACAA ATAGTAATAA TAATCTGTGC AACGGCACAG CAAACAACCC CTGGCCAATC 
AGCAGAAGCA GCAATGCAAG CACAGCCAAT CCTGTCAGCA CGAGCCCTAC GGCGACTCAG 
CACTCAGCCA ATCAGCAGAA CTCTCCCAAC GATTCTGGAA TCTCATTGGC TGACGGGGAT 
CTGTTGGGCG CAGCTGTGAA AACAAGGGAA CAGCTCTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID ODa29027
Clone ID Related Accession (Same CDS sequence) XM_684006.8 , XM_684006.9
Accession Version XM_684006.9 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 2019bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2017-06-14
Organism Danio rerio(zebrafish)
Product bestrophin-1 isoform X1
Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007136.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_684006.8. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
ATGACGGTGA CGTACTCGCG GCGGGTGGCA GATGCGCGTC TGGGCACCTT TTATCGCCTT 
CTTCTGCGAT GGAAAGGAAG CATTTATAAG CTGCTGTATC GCGAGCTGCT GATCTTCACT 
GTGATGTACT GCATTATCAG CATCACATAC AGGTGTGTAC TGACTGAAGA GCAGCGCAGG 
ATGTTTGAGA AGCTCTCCCT GTACTGTGAT CAGTACGCTC AGCTCATTCC TGTGTCATTC 
GTACTGGGTT TCTATGTGAC TCTGGTGGTG TCCCGCTGGT GGGGTCAGTT CGAGAGCGTC 
CCCTGGCCGG ACCGCCTGTC AGCTCTGGTC AGTGGACATG TACGGGGTGC AGATGAAGGA 
GCCAGACTCA TACGCAGGAG TTTAATGCGC TACGCAAACC TGTCCGGCAT CCTCATCTAC 
CGCTCCGTCA GCACGGCTGT CTACAAGAGA TTCCCGACCA TGAGCCACTT GGTTCAAGCA 
GGTCTGATGA CAGCGGAGGA GCTGAGGCAG CTGCAGGAGC TTCCTTCACC TCATAATAAG 
TTCTGGGTTC CCTGCATGTG GTTTGTCAGT CTGGCTATGA GAGCTCGATC TGAAGGACGA 
ATCAATAACG ACGTCGCAAT GACAGCCATC CTCAACGAAC TGAACACGCT TCGGTCTCAA 
TGTATGAGGC TGTACGGATA TGATTGGATC AGCTTACCTC TCGTTTATAC ACAGGTGGTA 
ACCGTGGCTG TGTACAGTTT CTTTTTGACT TGTCTGATTG GTCGGCAGTT TCTTGACCCC 
GCCCAGGGTT ACCCGGGTCA CAACCTCGAC TTCTACCTGC CTGTCTTCAC TTTACTCCAG 
TTCTTCTTCT ACGTGGGCTG GCTTAAGGTT GCAGAGCAGC TTATTAATCC ATTTGGGGAG 
GATGATGACG ACTTTGAGAC TAACTGGCTG GTGGACCGTA ATTTACAGGT GTCTCTGCTG 
TCAGTGGATG AGATGTATGA TCTGGTTCCT CTGGTTGAGA GAGATAAATA CTGGGATGAA 
GCTGAGCCTC AGCCGCCGTA CACCGCCGCC AGCGCAGAAC ACCGCAAACC CTCATTCATG 
GGATCTGCGC TGGACATCAG CGTTCCTAAA GAGGAAATGG AATTCCAGCA CAATCTGGAG 
CAGATTAAAG AGCACGAGGA GGCCAATCAC TCCACTCCAT TCCTGGGCAG CTTGAGTCGA 
CTTCTGGGCG TTCAGTCGCC CAATTTCCCC CGCTCGACCC CTACATCAAG AGTCTCCCTC 
CTGCGACGCC GACCCCGTGC TCCGTTCTCT CGTTTCCCAC TTTACCTCCA TTCTGAGAGC 
TCACAGATGC CCAGCCACAG TCGCAACACG GATGTGAGCG AGGACGCCTT TTCAGCAATG 
CCTCTGTATG AACGACCCGG TTTTTACAGC TGCCCCCAAA CGCCCATCCA CTGCATCCCT 
CCACCCGTTC CACGACCAAG ACCACGGAGA GCGCATGGTG ACAGCTCCAG CTCCTTAACA 
CAACCTATGG TGGGCTCACA AATCCTGGCT CTGCCTGACA CACCCGCACC TCCTTCATCC 
GCCTTCCCTT GGGTGGGGGA AGACAGCGAA CATCCGGCGT TCTCCTTTCC AGATCCTGTC 
CCTGAAATAT GTCCGTTAAC CAAAGCACGA CTAATTCAAG GCTTACCATC GCGCCGTCCT 
CTCCCTCTGC GACTATCTGT CGATAAATCT CCATCTCCAT CTACACCAGA GAGGGTGTTT 
TCATTCACAC CGCCATCTTG GCCGCAAACG CAAATCAACA CCGCTAGTTC AGTGAATGTG 
AACGTGACAA ATAGTAATAA TAATCTGTGC AACGGCACAG CAAACAACCC CTGGCCAATC 
AGCAGAAGCA GCAATGCAAG CACAGCCAAT CCTGTCAGCA CGAGCCCTAC GGCGACTCAG 
CACTCAGCCA ATCAGCAGAA CTCTCCCAAC GATTCTGGAA TCTCATTGGC TGACGGGGAT 
CTGTTGGGCG CAGCTGTGAA AACAAGGGAA CAGCTCTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_689098.3
CDS131..2149
Translation

Target ORF information:

RefSeq Version XM_684006.9
Organism Danio rerio(zebrafish)
Definition Danio rerio bestrophin 1 (best1), transcript variant X1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 XM_684006.9

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
ATGACGGTGA CGTACTCGCG GCGGGTGGCA GATGCGCGTC TGGGCACCTT TTATCGCCTT 
CTTCTGCGAT GGAAAGGAAG CATTTATAAG CTGCTGTATC GCGAGCTGCT GATCTTCACT 
GTGATGTACT GCATTATCAG CATCACATAC AGGTGTGTAC TGACTGAAGA GCAGCGCAGG 
ATGTTTGAGA AGCTCTCCCT GTACTGTGAT CAGTACGCTC AGCTCATTCC TGTGTCATTC 
GTACTGGGTT TCTATGTGAC TCTGGTGGTG TCCCGCTGGT GGGGTCAGTT CGAGAGCGTC 
CCCTGGCCGG ACCGCCTGTC AGCTCTGGTC AGTGGACATG TACGGGGTGC AGATGAAGGA 
GCCAGACTCA TACGCAGGAG TTTAATGCGC TACGCAAACC TGTCCGGCAT CCTCATCTAC 
CGCTCCGTCA GCACGGCTGT CTACAAGAGA TTCCCGACCA TGAGCCACTT GGTTCAAGCA 
GGTCTGATGA CAGCGGAGGA GCTGAGGCAG CTGCAGGAGC TTCCTTCACC TCATAATAAG 
TTCTGGGTTC CCTGCATGTG GTTTGTCAGT CTGGCTATGA GAGCTCGATC TGAAGGACGA 
ATCAATAACG ACGTCGCAAT GACAGCCATC CTCAACGAAC TGAACACGCT TCGGTCTCAA 
TGTATGAGGC TGTACGGATA TGATTGGATC AGCTTACCTC TCGTTTATAC ACAGGTGGTA 
ACCGTGGCTG TGTACAGTTT CTTTTTGACT TGTCTGATTG GTCGGCAGTT TCTTGACCCC 
GCCCAGGGTT ACCCGGGTCA CAACCTCGAC TTCTACCTGC CTGTCTTCAC TTTACTCCAG 
TTCTTCTTCT ACGTGGGCTG GCTTAAGGTT GCAGAGCAGC TTATTAATCC ATTTGGGGAG 
GATGATGACG ACTTTGAGAC TAACTGGCTG GTGGACCGTA ATTTACAGGT GTCTCTGCTG 
TCAGTGGATG AGATGTATGA TCTGGTTCCT CTGGTTGAGA GAGATAAATA CTGGGATGAA 
GCTGAGCCTC AGCCGCCGTA CACCGCCGCC AGCGCAGAAC ACCGCAAACC CTCATTCATG 
GGATCTGCGC TGGACATCAG CGTTCCTAAA GAGGAAATGG AATTCCAGCA CAATCTGGAG 
CAGATTAAAG AGCACGAGGA GGCCAATCAC TCCACTCCAT TCCTGGGCAG CTTGAGTCGA 
CTTCTGGGCG TTCAGTCGCC CAATTTCCCC CGCTCGACCC CTACATCAAG AGTCTCCCTC 
CTGCGACGCC GACCCCGTGC TCCGTTCTCT CGTTTCCCAC TTTACCTCCA TTCTGAGAGC 
TCACAGATGC CCAGCCACAG TCGCAACACG GATGTGAGCG AGGACGCCTT TTCAGCAATG 
CCTCTGTATG AACGACCCGG TTTTTACAGC TGCCCCCAAA CGCCCATCCA CTGCATCCCT 
CCACCCGTTC CACGACCAAG ACCACGGAGA GCGCATGGTG ACAGCTCCAG CTCCTTAACA 
CAACCTATGG TGGGCTCACA AATCCTGGCT CTGCCTGACA CACCCGCACC TCCTTCATCC 
GCCTTCCCTT GGGTGGGGGA AGACAGCGAA CATCCGGCGT TCTCCTTTCC AGATCCTGTC 
CCTGAAATAT GTCCGTTAAC CAAAGCACGA CTAATTCAAG GCTTACCATC GCGCCGTCCT 
CTCCCTCTGC GACTATCTGT CGATAAATCT CCATCTCCAT CTACACCAGA GAGGGTGTTT 
TCATTCACAC CGCCATCTTG GCCGCAAACG CAAATCAACA CCGCTAGTTC AGTGAATGTG 
AACGTGACAA ATAGTAATAA TAATCTGTGC AACGGCACAG CAAACAACCC CTGGCCAATC 
AGCAGAAGCA GCAATGCAAG CACAGCCAAT CCTGTCAGCA CGAGCCCTAC GGCGACTCAG 
CACTCAGCCA ATCAGCAGAA CTCTCCCAAC GATTCTGGAA TCTCATTGGC TGACGGGGAT 
CTGTTGGGCG CAGCTGTGAA AACAAGGGAA CAGCTCTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

PubMed