ORF cDNA Clones and Custom Clones » Species Summary » Danio rerio » srd5a3 cDNA ORF clone

srd5a3 cDNA ORF clone, Danio rerio(zebrafish)

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Summary

Gene Symbol	srd5a3
Entrez Gene ID	560717
Full Name	steroid 5 alpha-reductase 3
Synonyms	S5AR 3,fj40g12,si:ch211-278f21.3,srd5a2l,wu:fj40g12
General protein information	Preferred Names steroid 5 alpha-reductase 3 Names polyprenol reductase 3-oxo-5-alpha-steroid 4-dehydrogenase 3 SR type 3 probable polyprenol reductase
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

Reference Sequences (RefSeq)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001044939.1	NP_001038404.1	polyprenol reductase precursor

Homologies

Pan troglodytes (chimpanzee)	SRD5A3	XP_526623.1
Canis lupus familiaris (dog)	SRD5A3	XP_539274.2
Mus musculus (house mouse)	Srd5a3	NP_065636.2
Gallus gallus (chicken)	SRD5A3	XP_420703.2
Homo sapiens (human)	SRD5A3	NP_078868.1
	SRD5A3	XP_001088853.1
Rattus norvegicus (Norway rat)	Srd5a3	NP_001014012.1
Caenorhabditis elegans (roundworm)	B0024.13	NP_001256209.1
Bos taurus (cattle)	SRD5A3	NP_001193019.1
Danio rerio (zebrafish)	srd5a3	NP_001038404.1
Drosophila melanogaster (fruit fly)	CG7840	NP_609203.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP007493	XP_308382.3
Xenopus tropicalis (tropical clawed frog)	srd5a3	NP_001072539.1

trappc11 is required for protein glycosylation in zebrafish and humans.
DeRossi C, Vacaru A, Rafiq R, Cinaroglu A, Imrie D, Nayar S, Baryshnikova A, Milev MP, Stanga D, Kadakia D, Gao N, Chu J, Freeze HH, Lehrman MA, Sacher M, Sadler KC
Molecular biology of the cell27(8)1220-34(2016 Apr)

RFX transcription factors are essential for hearing in mice.
Elkon R, Milon B, Morrison L, Shah M, Vijayakumar S, Racherla M, Leitch CC, Silipino L, Hadi S, Weiss-Gayet M, Barras E, Schmid CD, Ait-Lounis A, Barnes A, Song Y, Eisenman DJ, Eliyahu E, Frolenkov GI, Strome SE, Durand B, Zaghloul NA, Jones SM, Reith W, Hertzano R
Nature communications68549(2015 Oct)

kitb, a second zebrafish ortholog of mouse Kit.
Mellgren EM, Johnson SL
Development genes and evolution215(9)470-77(2005 Sep)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

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The following srd5a3 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the srd5a3 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

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***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ODa00115	NM_001044939.1 Latest version!	Danio rerio steroid 5 alpha-reductase 3 (srd5a3), mRNA.	pcDNA3.1⁺/C-(K)DYK or customized vector	7-9	$174.30 $249.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

srd5a3 ( NM_001044939.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_001038404.1 Danio rerio steroid 5 alpha-reductase 3 (srd5a3), mRNA.

Price & Availability↑

CloneID	ODa00115
Clone ID Related Accession (Same CDS sequence)	NM_001044939.1
Accession Version	NM_001044939.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 930bp) Protein sequence SNP
Vector	pcDNA3.1⁺/C-(K)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-28
Organism	Danio rerio(zebrafish)
Product	polyprenol reductase precursor
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BX088688.6. On Sep 1, 2006 this sequence version replaced XM_684116.1. ##Evidence-Data-START## Transcript exon combination :: BC117626.1, GFIL01039642.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA4476770, SAMN01765705 [ECO:0000348] ##Evidence-Data-END##

ATGTTTCATA TTTTAAGTAT AGTTAATATA ATATGGCTTT TGTTAGCGCT ATGTTTTGGT 
GCAGCCTTTT GCCTCAATAA GTTTTCAGTG AAACTGCCTA ACAGAGTCGA GCACGTGTTC 
CAGGATTTTA TCCGGTATGG AAAAACTAAG GAAAACATCA AACGCGCTAG CTGGCAGCTC 
GTTTTTGACT TATCAAAGAG GTATTTCTAT CATTTCTATG TGGTGTCTGT CATGTGGAAT 
GGGCTCCTCC TACTGTTTTC CATACGGTCT GTCGTAATGT CTGAGGCGTT CCCTGATTGG 
ATCATTGACG TACTTGGGAG TTTGACTGGC AGATCAAGGG GTGCTTGGAA CGAGATACAT 
CTGTCCACTC TGCTTTTACA AGTGCTCCTA TGGGTTCATA CACTAAGACG ACTGTTGGAG 
TGCCTGTTTG TCAGCGTATT CTCTGATGGT GTGATCAATG TGGTTCAATA TGCATTTGGC 
TTGAGTTATT ATATCATACT TGGACTAACT GTGCTGTGTA CAAATGATTC TCTTCCACAG 
TCAGAGTCAG TTTCTTTCTT TAATCAGCTA ACATGGTATC ATGTAGTCGG GACTCTTCTC 
TTCTTCTGGG CCTCATTTCT CCAGCACCAG TCCCTCTCTC TGCTGGCCAA GATGAGGACT 
GACAGCTCAG GTAAAGTAGA GACTCTGGCC CACAAGATGC CGTGTGGCGG TTGGTTTGAG 
CTGGTCTCTT GTCCACATTA CCTGGCTGAG CTTCTGATTT ATGCTGCCAT GTGTGTTTGC 
TGTGGATGTG CTTCACTAAC CTGGTGGATG GTGGTGCTGT ACGTTCTCTG TAACCAAGCA 
CTTGCTGCAC AGCTCTGTCA TGAGTACTAC AGGAGCAAGT TTAAGACATA TCCACATCAC 
CGAAAAGCTT TCATCCCTTT TGTCCTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001038404.1
CDS	39..968
Translation	MFHILSIVNIIWLLLALCFGAAFCLNKFSVKLPNRVEHVFQDFIRYGKTKENIKRASWQLVFDLSKRYFYHFYVVSVMWNGLLLLFSIRSVVMSEAFPDWIIDVLGSLTGRSRGAWNEIHLSTLLLQVLLWVHTLRRLLECLFVSVFSDGVINVVQYAFGLSYYIILGLTVLCTNDSLPQSESVSFFNQLTWYHVVGTLLFFWASFLQHQSLSLLAKMRTDSSGKVETLAHKMPCGGWFELVSCPHYLAELLIYAAMCVCCGCASLTWWMVVLYVLCNQALAAQLCHEYYRSKFKTYPHHRKAFIPFVL

Target ORF information:

RefSeq Version	NM_001044939.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio steroid 5 alpha-reductase 3 (srd5a3), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001044939.1

ORF Insert Sequence:

ATGTTTCATA TTTTAAGTAT AGTTAATATA ATATGGCTTT TGTTAGCGCT ATGTTTTGGT 
GCAGCCTTTT GCCTCAATAA GTTTTCAGTG AAACTGCCTA ACAGAGTCGA GCACGTGTTC 
CAGGATTTTA TCCGGTATGG AAAAACTAAG GAAAACATCA AACGCGCTAG CTGGCAGCTC 
GTTTTTGACT TATCAAAGAG GTATTTCTAT CATTTCTATG TGGTGTCTGT CATGTGGAAT 
GGGCTCCTCC TACTGTTTTC CATACGGTCT GTCGTAATGT CTGAGGCGTT CCCTGATTGG 
ATCATTGACG TACTTGGGAG TTTGACTGGC AGATCAAGGG GTGCTTGGAA CGAGATACAT 
CTGTCCACTC TGCTTTTACA AGTGCTCCTA TGGGTTCATA CACTAAGACG ACTGTTGGAG 
TGCCTGTTTG TCAGCGTATT CTCTGATGGT GTGATCAATG TGGTTCAATA TGCATTTGGC 
TTGAGTTATT ATATCATACT TGGACTAACT GTGCTGTGTA CAAATGATTC TCTTCCACAG 
TCAGAGTCAG TTTCTTTCTT TAATCAGCTA ACATGGTATC ATGTAGTCGG GACTCTTCTC 
TTCTTCTGGG CCTCATTTCT CCAGCACCAG TCCCTCTCTC TGCTGGCCAA GATGAGGACT 
GACAGCTCAG GTAAAGTAGA GACTCTGGCC CACAAGATGC CGTGTGGCGG TTGGTTTGAG 
CTGGTCTCTT GTCCACATTA CCTGGCTGAG CTTCTGATTT ATGCTGCCAT GTGTGTTTGC 
TGTGGATGTG CTTCACTAAC CTGGTGGATG GTGGTGCTGT ACGTTCTCTG TAACCAAGCA 
CTTGCTGCAC AGCTCTGTCA TGAGTACTAC AGGAGCAAGT TTAAGACATA TCCACATCAC 
CGAAAAGCTT TCATCCCTTT TGTCCTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

trappc11 is required for protein glycosylation in zebrafish and humans.
Molecular biology of the cell27(8)1220-34(2016 Apr)
DeRossi C,Vacaru A,Rafiq R,Cinaroglu A,Imrie D,Nayar S,Baryshnikova A,Milev MP,Stanga D,Kadakia D,Gao N,Chu J,Freeze HH,Lehrman MA,Sacher M,Sadler KC

RFX transcription factors are essential for hearing in mice.
Nature communications68549(2015 Oct)
Elkon R,Milon B,Morrison L,Shah M,Vijayakumar S,Racherla M,Leitch CC,Silipino L,Hadi S,Weiss-Gayet M,Barras E,Schmid CD,Ait-Lounis A,Barnes A,Song Y,Eisenman DJ,Eliyahu E,Frolenkov GI,Strome SE,Durand B,Zaghloul NA,Jones SM,Reith W,Hertzano R

kitb, a second zebrafish ortholog of mouse Kit.
Development genes and evolution215(9)470-77(2005 Sep)
Mellgren EM,Johnson SL

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srd5a3 cDNA ORF clone, Danio rerio(zebrafish)

Summary

Reference Sequences (RefSeq)

Pathways from BioSystems

Homologies

Gene Ontology

Bibliography

Reference Sequences (Refseq)

Target ORF information:

Target ORF information:

ORF Insert Sequence:

PubMed

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