ahi1 cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Gene Symbol	ahi1
Entrez Gene ID	562701
Full Name	Abelson helper integration site 1
General protein information	Preferred Names Abelson helper integration site 1 Names jouberin ZAhi-1
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA Protein Name

NM_001319989.1 NP_001306918.1 jouberin
Pathways from BioSystems

mRNA	Protein	Name
NM_001319989.1	NP_001306918.1	jouberin

Homologies

Homo sapiens (human)	AHI1	NP_001128303.1
Rattus norvegicus (Norway rat)	Ahi1	NP_001002277.1
Mus musculus (house mouse)	Ahi1	NP_080479.2
Gallus gallus (chicken)	AHI1	XP_004935779.1
	AHI1	XP_001099136.2
Canis lupus familiaris (dog)	AHI1	XP_005615497.1
Xenopus tropicalis (tropical clawed frog)	ahi1	XP_004914630.1
Pan troglodytes (chimpanzee)	AHI1	XP_527511.3
Bos taurus (cattle)	AHI1	XP_002690311.1
Danio rerio (zebrafish)	ahi1	NP_001071029.1

Gene Ontology
Bibliography

Related articles in PubMed

The Ciliopathy Gene ahi1 Is Required for Zebrafish Cone Photoreceptor Outer Segment Morphogenesis and Survival.
Lessieur EM, Fogerty J, Gaivin RJ, Song P, Perkins BD
Investigative ophthalmology & visual science58(1)448-460(2017 01)

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
Pasquier J, Cabau C, Nguyen T, Jouanno E, Severac D, Braasch I, Journot L, Pontarotti P, Klopp C, Postlethwait JH, Guiguen Y, Bobe J
BMC genomics17368(2016 05)

RFX transcription factors are essential for hearing in mice.
Elkon R, Milon B, Morrison L, Shah M, Vijayakumar S, Racherla M, Leitch CC, Silipino L, Hadi S, Weiss-Gayet M, Barras E, Schmid CD, Ait-Lounis A, Barnes A, Song Y, Eisenman DJ, Eliyahu E, Frolenkov GI, Strome SE, Durand B, Zaghloul NA, Jones SM, Reith W, Hertzano R
Nature communications68549(2015 Oct)

Non-manifesting AHI1 truncations indicate localized loss-of-function tolerance in a severe Mendelian disease gene.
Elsayed SM, Phillips JB, Heller R, Thoenes M, Elsobky E, N?rnberg G, N?rnberg P, Seland S, Ebermann I, Altm?ller J, Thiele H, Toliat M, K?rber F, Hu XJ, Wu YD, Zaki MS, Abdel-Salam G, Gleeson J, Boltshauser E, Westerfield M, Bolz HJ
Human molecular genetics24(9)2594-603(2015 May)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The ahi1lri46 mutation in zebrafish resulted in shorter cone outer segments but did not affect visual behavior at 5 days after fertilization (dpf).
Title: The Ciliopathy Gene ahi1 Is Required for Zebrafish Cone Photoreceptor Outer Segment Morphogenesis and Survival.

a role for Ahi1 in epithelial cell organization and ciliary formation and explain the ciliopathy phenotype of AHI1 mutations in man.
Title: Modelling a ciliopathy: Ahi1 knockdown in model systems reveals an essential role in brain, retinal, and renal development.

Results describe spatiotemporal expression patterns of AHI1 and orthologs throughout development, in human, mouse, and zebrafish, and suggest roles for AHI1 in neurodevelopmental processes behind most of the neuroanatomical defects in Joubert syndrome.
Title: Species differences in the expression of Ahi1, a protein implicated in the neurodevelopmental disorder Joubert syndrome, with preferential accumulation to stigmoid bodies.

We cloned the full cDNA sequence of Ahi-1 homologous in zebrafish.RT-PCR reveal that ZAhi-1 expressed highest in the mature gonad. In situ hybridization results of zebrafish gonad show that ZAhi-1 only expressed in the early stages' gamete cells.[ZAhi-1]
Title: Molecular cloning of a novel gene ZAhi-1 and its expression analysis during zebrafish gametogenesis.

The following ahi1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ahi1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ODa43867	NM_001319989.1 Latest version!	Danio rerio Abelson helper integration site 1 (ahi1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

ahi1 ( NM_001319989.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_001306918.1 Danio rerio Abelson helper integration site 1 (ahi1), mRNA.

Price & Availability↑

CloneID	ODa43867
Clone ID Related Accession (Same CDS sequence)	NM_001319989.1
Accession Version	NM_001319989.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3573bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2020-01-04
Organism	Danio rerio(zebrafish)
Product	jouberin
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from EB855290.1, EH466743.1, BX248131.6, EB931323.1 and CR407704.10. On Feb 9, 2016 this sequence version replaced XM_009292820.1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data because no single transcript from the reference strain was available for the full length of the gene. The extent of this transcript is supported by transcript alignments and orthologous data. ##Evidence-Data-START## Transcript exon combination :: GDQH01019081.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA2168446, SAMEA2168447 [ECO:0000350] ##Evidence-Data-END##

ATGCCAGCAG GTCAAAGTGA TGTGAGAGCT AAGACGAAAG CTCGCTTTGA CGAGGTTTTG 
AAGAGATACA CAAACCCTCC ATCTGAGAAA AAGAAAGCCA AACAGATCTC TGACCAGTCT 
CAGGAAATTA TCGTGCTTCA GACCTTAAAG AAGAACCTAG ATTTGAGTAA AGACACTGAA 
GATGATGAAG CCATCCTACA TAACACATAC GATCCGGCTC AGGGCAGCCC TAGATACACC 
AAAAACAGGC GCAGGGAGAG GGAAGTATCA GAAAAGACTA ACTTAAACAA TGCTGTGAGT 
GAGGAGGAGT CCACATTGAC CAGCGGGAAG AGGAAAAGCA AGAGGAAGCT CCCACAACCA 
CCACCCAGTG TTCAAGACGC AGATGACAAT GACAGAAGGA CTGATGCAGA AACTGATGGA 
AAACCTATTA GGAGGATAAA AAAAGGGAAA AACAAAGCTG CTAATGACTC GAGTCAACAG 
AAAGAGCAGA CGGAGACAGA AGATGATTAT TTACGAGCGT ACCAACATCA GATCTCACAG 
GAAGAAGAGA CGGCGGGAAA GAAATCGACA CGAAAGAAAG CCGCAGAGAA GCTTGAGGAG 
AAGCAGCTGC TAAACAATGA GCTGGAGAAG AAAAAGAAGA AGGCGAAATC AAGCAAACAT 
GATGCTGAAA GAAGTCAGGC TGAAGTGTCA GCAGAAAACC AGACAGAAAT CATCCAGGAT 
GATGTAGAGG AGTTCAAGGA GGATACAGTC AAACCCAAAG TGAAGAAGAA AAAGAAACGA 
AAAGAAGTGC CTGTTGAGGA GGAGCAGACA GAAGAAAAAC CAGCCTTTGA TGACAGTTTG 
GTCCTGGGAG TTTATATCCA CAGAACAGAC AAGCTGAAGA CCGACCTGAT GGTGTCTCAC 
CCCATGGTCA AAGTTCATGT GGTTGATGAG GTCACTGGAC AGTATGTCAA GAAGGAGGAC 
AGCCATCGTC ATGTTTCATC CTTCTATGAG CAAGAAAACA TTGAGCACAT CCTGCCCATT 
ATAACTCAAC CATTTGACTT CAAGAAACAC AAAACAACAG TTCCAGAGTG GGAGGAACAG 
ATCATCTTCA ATGAGCGATT CGGATATTTT CTACAAGATG ACGACGAGAG TCCACGTGTG 
ATGCTGCTTT TTGAGATTCT GGATTTCATC AGTATGGAGG AGGCTCGAAC AAACGTCTCA 
GTTGATCGAC ATGAAAGAGG TTTCAGAAAG ATTGCTTGGG CTTTTTTGAA GCTTGTAGGA 
ACCAATGGGG TGTTGAACAT TGACACCAAA CTGCGTCTGC AGCTTTACTG CCCTCCTCCA 
CGAGCGAAGA AAGTACCACA AACGGTGGAA GTCTTTCAAT GGTGGTCAAA GTACCCAAGG 
AACAAATACA CCTCAACTCT CTATGTCACC GTGAAAGGCC TGAAACCACC AGAACATGTT 
GACCCCGGTA TCCGCTCCAT GATGGCTCTG CAGCAGGAGC GCGGCAGCAC CTCCTACAGT 
GAGCTCAATT CTGAGATGAC CAATAAAACC AGCACTCAGA TACAGGAGAG CAAAGCAGAA 
ACCATGAAGT GGAGCCGTAT GCCCGGACAG GTGTGCCGGA TCCCAAATAA GCCAATGCAC 
TCTTTCCGTG GAGGTCAGAT GGGCTGTTTT ACTCTCTGTT TCTCACATGA TGGACGTGCG 
CTAGCAGCTG CGTGTGCAGA TAGAGATGCT TTTCCTATCA TAATATATGA GATTCCATCT 
GGGAAAGTTC TGTCATCTTT TAATGGCCAT CTGAGTGTGG TTTATGATCT CTGCTGGTCC 
AGAGATGATA AAGGCCTGTT AACTGCATCC TCTGATGGAA CAGTGAGAGT GTGGAATGTT 
GAACGTCTGC AGACTCTTGC GCATAAAACT CTTCCGCACC CTTCATTCGT GTACTGTGCC 
AAGTTCCACC CTCAAGCCCA GAGTCTGGTG GCGACGGGGG GTTACGATGG CGTGCTGCGA 
GTCTGGAACG TAGATGTACA AGATGTGAAT GGTCAACTTT TGCAAGAGTT TGACGGACAC 
AAGACTTTCA TCAACACTCT GTGTTTTGAC CCTGAAGGGA GCAGAATGTT TTCTGCTGAT 
AATTCCGGAC TCATCATTGT GTGGGGAACG AAAGTTATGC CTGGGTCTCG CCGTGGAGCA 
ACCAGTCAGT GGAGCATTGA GAGGGAAATT AAAGAGGCAG ATCTGAACGG AATATCTATT 
AACTCATTAG AGGTGCATCC AAATGGACGA AGACTCTTAA TTCATGCCAA AGACAGCGTT 
TTGAGGGTCA TGGATTTGAG AATTCTGGCA GTGAAGAAAT ACATAGGTGC CACAAACTAC 
AGGGATCGAA TCAACAGTAC GTTCACTCCT TGCGGAAGCT TCATCTTCGC TGGTAGTGAA 
GATGGACTGG CGTATGTCTG GAACTCTGAG ACAGGTGACC AGGTTGCCGT GTACTCAGAG 
CTGTGTTACT CATCTGCACT TCGAGCTGTA GCTTTCCACC CTCATGAGAA CATGGTGGCC 
TTCTGTTGCT TCGGCCAAAA CCAGCCCATC CATCTCTATC TGTATGACCG TAAAGTGGCT 
CAGATGGAGG TGGAGAGTTT GCGAGGCTGG AGTCGCTCAG AGAATCTGGA CAGTAAGACG 
GGCAGGATTT CATCAGAGTT CATGAGCATC CAGAACCCCA CGGCCACCAG CATGGACCGA 
TTTGCCAACA CTGCACGCAT GTCCCTGAAG ATGCAGCGGG TCAAACAGAA ACTAGACTCT 
GTGCTGGAGC CTCATCGCAG TTCCTCTAAT GTGGACATGT ATGAGCAAGG TGGAATGTCT 
TCCTTGGGAA GGAGTATAGA GGGCCTGAAC TCATATCTTC CTCCTCCATC TCTACTGTCT 
CCTCACTCTA AACTGCAGAT GTCTTCTTCA CTGAGCGCTC AGCTTATTCC TCAGGCCACA 
CTGACCTCAC ACACCAGTGG TTACAGTCAA GCCGGACAGC CAATCAGAAG AGCTCCATCC 
CTCAGACAGC AGAAAAGTTT CCATGATTAT GAATTCTCTC AACAAGTAGA GACAGATTTG 
GGTCAAGCTC ATCAGACGGT TGTGTCTTTA TACGAGTACA GAGCCAATAG GTCGGACGAG 
CTGTCGATGC GGCGGGGTGA CGTGGTTCAT GTGCTGTATA AAGACAATGA CAGCTGGTGG 
TTTGGCCGAC TGGTCAGTGG GCAGGAGGGA TATTTCCCAG CATCTTATGT GGCTGATGAA 
GGGAACTTTG ATGAGGAGCT GTCAAGAGCT TTAGAAGCAC AACCGTCACT GATAGATCAA 
CCTGAGGAAG CGGAGCATGC AACACCAGTT CAGATGTCCG CAGCCATCAG TGCCTCTGGA 
GAGCTGAAGA TCATCTCAGA GCTGGACACG GATGCTGAAG CCTCCTCCAC CACAAGAAAA 
AAGAAGAAGA AAAAGGTGAA GCGGATTGAA GTTCCCTCTG AAGATCTGCC TCCATCTTCA 
GGATCTTCCA GAAGAAGGAG ACCTCTTCCC AAACTCGGCC AAGCCAACGG TGCGTTTGAA 
GGTGAAGGAT TGAACTTACG GGATCTGGTC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001306918.1
CDS	143..3715
Translation	MPAGQSDVRAKTKARFDEVLKRYTNPPSEKKKAKQISDQSQEIIVLQTLKKNLDLSKDTEDDEAILHNTYDPAQGSPRYTKNRRREREVSEKTNLNNAVSEEESTLTSGKRKSKRKLPQPPPSVQDADDNDRRTDAETDGKPIRRIKKGKNKAANDSSQQKEQTETEDDYLRAYQHQISQEEETAGKKSTRKKAAEKLEEKQLLNNELEKKKKKAKSSKHDAERSQAEVSAENQTEIIQDDVEEFKEDTVKPKVKKKKKRKEVPVEEEQTEEKPAFDDSLVLGVYIHRTDKLKTDLMVSHPMVKVHVVDEVTGQYVKKEDSHRHVSSFYEQENIEHILPIITQPFDFKKHKTTVPEWEEQIIFNERFGYFLQDDDESPRVMLLFEILDFISMEEARTNVSVDRHERGFRKIAWAFLKLVGTNGVLNIDTKLRLQLYCPPPRAKKVPQTVEVFQWWSKYPRNKYTSTLYVTVKGLKPPEHVDPGIRSMMALQQERGSTSYSELNSEMTNKTSTQIQESKAETMKWSRMPGQVCRIPNKPMHSFRGGQMGCFTLCFSHDGRALAAACADRDAFPIIIYEIPSGKVLSSFNGHLSVVYDLCWSRDDKGLLTASSDGTVRVWNVERLQTLAHKTLPHPSFVYCAKFHPQAQSLVATGGYDGVLRVWNVDVQDVNGQLLQEFDGHKTFINTLCFDPEGSRMFSADNSGLIIVWGTKVMPGSRRGATSQWSIEREIKEADLNGISINSLEVHPNGRRLLIHAKDSVLRVMDLRILAVKKYIGATNYRDRINSTFTPCGSFIFAGSEDGLAYVWNSETGDQVAVYSELCYSSALRAVAFHPHENMVAFCCFGQNQPIHLYLYDRKVAQMEVESLRGWSRSENLDSKTGRISSEFMSIQNPTATSMDRFANTARMSLKMQRVKQKLDSVLEPHRSSSNVDMYEQGGMSSLGRSIEGLNSYLPPPSLLSPHSKLQMSSSLSAQLIPQATLTSHTSGYSQAGQPIRRAPSLRQQKSFHDYEFSQQVETDLGQAHQTVVSLYEYRANRSDELSMRRGDVVHVLYKDNDSWWFGRLVSGQEGYFPASYVADEGNFDEELSRALEAQPSLIDQPEEAEHATPVQMSAAISASGELKIISELDTDAEASSTTRKKKKKKVKRIEVPSEDLPPSSGSSRRRRPLPKLGQANGAFEGEGLNLRDLV

Target ORF information:

RefSeq Version	NM_001319989.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio Abelson helper integration site 1 (ahi1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001319989.1

ORF Insert Sequence:

ATGCCAGCAG GTCAAAGTGA TGTGAGAGCT AAGACGAAAG CTCGCTTTGA CGAGGTTTTG 
AAGAGATACA CAAACCCTCC ATCTGAGAAA AAGAAAGCCA AACAGATCTC TGACCAGTCT 
CAGGAAATTA TCGTGCTTCA GACCTTAAAG AAGAACCTAG ATTTGAGTAA AGACACTGAA 
GATGATGAAG CCATCCTACA TAACACATAC GATCCGGCTC AGGGCAGCCC TAGATACACC 
AAAAACAGGC GCAGGGAGAG GGAAGTATCA GAAAAGACTA ACTTAAACAA TGCTGTGAGT 
GAGGAGGAGT CCACATTGAC CAGCGGGAAG AGGAAAAGCA AGAGGAAGCT CCCACAACCA 
CCACCCAGTG TTCAAGACGC AGATGACAAT GACAGAAGGA CTGATGCAGA AACTGATGGA 
AAACCTATTA GGAGGATAAA AAAAGGGAAA AACAAAGCTG CTAATGACTC GAGTCAACAG 
AAAGAGCAGA CGGAGACAGA AGATGATTAT TTACGAGCGT ACCAACATCA GATCTCACAG 
GAAGAAGAGA CGGCGGGAAA GAAATCGACA CGAAAGAAAG CCGCAGAGAA GCTTGAGGAG 
AAGCAGCTGC TAAACAATGA GCTGGAGAAG AAAAAGAAGA AGGCGAAATC AAGCAAACAT 
GATGCTGAAA GAAGTCAGGC TGAAGTGTCA GCAGAAAACC AGACAGAAAT CATCCAGGAT 
GATGTAGAGG AGTTCAAGGA GGATACAGTC AAACCCAAAG TGAAGAAGAA AAAGAAACGA 
AAAGAAGTGC CTGTTGAGGA GGAGCAGACA GAAGAAAAAC CAGCCTTTGA TGACAGTTTG 
GTCCTGGGAG TTTATATCCA CAGAACAGAC AAGCTGAAGA CCGACCTGAT GGTGTCTCAC 
CCCATGGTCA AAGTTCATGT GGTTGATGAG GTCACTGGAC AGTATGTCAA GAAGGAGGAC 
AGCCATCGTC ATGTTTCATC CTTCTATGAG CAAGAAAACA TTGAGCACAT CCTGCCCATT 
ATAACTCAAC CATTTGACTT CAAGAAACAC AAAACAACAG TTCCAGAGTG GGAGGAACAG 
ATCATCTTCA ATGAGCGATT CGGATATTTT CTACAAGATG ACGACGAGAG TCCACGTGTG 
ATGCTGCTTT TTGAGATTCT GGATTTCATC AGTATGGAGG AGGCTCGAAC AAACGTCTCA 
GTTGATCGAC ATGAAAGAGG TTTCAGAAAG ATTGCTTGGG CTTTTTTGAA GCTTGTAGGA 
ACCAATGGGG TGTTGAACAT TGACACCAAA CTGCGTCTGC AGCTTTACTG CCCTCCTCCA 
CGAGCGAAGA AAGTACCACA AACGGTGGAA GTCTTTCAAT GGTGGTCAAA GTACCCAAGG 
AACAAATACA CCTCAACTCT CTATGTCACC GTGAAAGGCC TGAAACCACC AGAACATGTT 
GACCCCGGTA TCCGCTCCAT GATGGCTCTG CAGCAGGAGC GCGGCAGCAC CTCCTACAGT 
GAGCTCAATT CTGAGATGAC CAATAAAACC AGCACTCAGA TACAGGAGAG CAAAGCAGAA 
ACCATGAAGT GGAGCCGTAT GCCCGGACAG GTGTGCCGGA TCCCAAATAA GCCAATGCAC 
TCTTTCCGTG GAGGTCAGAT GGGCTGTTTT ACTCTCTGTT TCTCACATGA TGGACGTGCG 
CTAGCAGCTG CGTGTGCAGA TAGAGATGCT TTTCCTATCA TAATATATGA GATTCCATCT 
GGGAAAGTTC TGTCATCTTT TAATGGCCAT CTGAGTGTGG TTTATGATCT CTGCTGGTCC 
AGAGATGATA AAGGCCTGTT AACTGCATCC TCTGATGGAA CAGTGAGAGT GTGGAATGTT 
GAACGTCTGC AGACTCTTGC GCATAAAACT CTTCCGCACC CTTCATTCGT GTACTGTGCC 
AAGTTCCACC CTCAAGCCCA GAGTCTGGTG GCGACGGGGG GTTACGATGG CGTGCTGCGA 
GTCTGGAACG TAGATGTACA AGATGTGAAT GGTCAACTTT TGCAAGAGTT TGACGGACAC 
AAGACTTTCA TCAACACTCT GTGTTTTGAC CCTGAAGGGA GCAGAATGTT TTCTGCTGAT 
AATTCCGGAC TCATCATTGT GTGGGGAACG AAAGTTATGC CTGGGTCTCG CCGTGGAGCA 
ACCAGTCAGT GGAGCATTGA GAGGGAAATT AAAGAGGCAG ATCTGAACGG AATATCTATT 
AACTCATTAG AGGTGCATCC AAATGGACGA AGACTCTTAA TTCATGCCAA AGACAGCGTT 
TTGAGGGTCA TGGATTTGAG AATTCTGGCA GTGAAGAAAT ACATAGGTGC CACAAACTAC 
AGGGATCGAA TCAACAGTAC GTTCACTCCT TGCGGAAGCT TCATCTTCGC TGGTAGTGAA 
GATGGACTGG CGTATGTCTG GAACTCTGAG ACAGGTGACC AGGTTGCCGT GTACTCAGAG 
CTGTGTTACT CATCTGCACT TCGAGCTGTA GCTTTCCACC CTCATGAGAA CATGGTGGCC 
TTCTGTTGCT TCGGCCAAAA CCAGCCCATC CATCTCTATC TGTATGACCG TAAAGTGGCT 
CAGATGGAGG TGGAGAGTTT GCGAGGCTGG AGTCGCTCAG AGAATCTGGA CAGTAAGACG 
GGCAGGATTT CATCAGAGTT CATGAGCATC CAGAACCCCA CGGCCACCAG CATGGACCGA 
TTTGCCAACA CTGCACGCAT GTCCCTGAAG ATGCAGCGGG TCAAACAGAA ACTAGACTCT 
GTGCTGGAGC CTCATCGCAG TTCCTCTAAT GTGGACATGT ATGAGCAAGG TGGAATGTCT 
TCCTTGGGAA GGAGTATAGA GGGCCTGAAC TCATATCTTC CTCCTCCATC TCTACTGTCT 
CCTCACTCTA AACTGCAGAT GTCTTCTTCA CTGAGCGCTC AGCTTATTCC TCAGGCCACA 
CTGACCTCAC ACACCAGTGG TTACAGTCAA GCCGGACAGC CAATCAGAAG AGCTCCATCC 
CTCAGACAGC AGAAAAGTTT CCATGATTAT GAATTCTCTC AACAAGTAGA GACAGATTTG 
GGTCAAGCTC ATCAGACGGT TGTGTCTTTA TACGAGTACA GAGCCAATAG GTCGGACGAG 
CTGTCGATGC GGCGGGGTGA CGTGGTTCAT GTGCTGTATA AAGACAATGA CAGCTGGTGG 
TTTGGCCGAC TGGTCAGTGG GCAGGAGGGA TATTTCCCAG CATCTTATGT GGCTGATGAA 
GGGAACTTTG ATGAGGAGCT GTCAAGAGCT TTAGAAGCAC AACCGTCACT GATAGATCAA 
CCTGAGGAAG CGGAGCATGC AACACCAGTT CAGATGTCCG CAGCCATCAG TGCCTCTGGA 
GAGCTGAAGA TCATCTCAGA GCTGGACACG GATGCTGAAG CCTCCTCCAC CACAAGAAAA 
AAGAAGAAGA AAAAGGTGAA GCGGATTGAA GTTCCCTCTG AAGATCTGCC TCCATCTTCA 
GGATCTTCCA GAAGAAGGAG ACCTCTTCCC AAACTCGGCC AAGCCAACGG TGCGTTTGAA 
GGTGAAGGAT TGAACTTACG GGATCTGGTC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The Ciliopathy Gene ahi1 Is Required for Zebrafish Cone Photoreceptor Outer Segment Morphogenesis and Survival.
Investigative ophthalmology & visual science58(1)448-460(2017 01)
Lessieur EM,Fogerty J,Gaivin RJ,Song P,Perkins BD

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
BMC genomics17368(2016 05)
Pasquier J,Cabau C,Nguyen T,Jouanno E,Severac D,Braasch I,Journot L,Pontarotti P,Klopp C,Postlethwait JH,Guiguen Y,Bobe J

RFX transcription factors are essential for hearing in mice.
Nature communications68549(2015 Oct)
Elkon R,Milon B,Morrison L,Shah M,Vijayakumar S,Racherla M,Leitch CC,Silipino L,Hadi S,Weiss-Gayet M,Barras E,Schmid CD,Ait-Lounis A,Barnes A,Song Y,Eisenman DJ,Eliyahu E,Frolenkov GI,Strome SE,Durand B,Zaghloul NA,Jones SM,Reith W,Hertzano R

Non-manifesting AHI1 truncations indicate localized loss-of-function tolerance in a severe Mendelian disease gene.
Human molecular genetics24(9)2594-603(2015 May)
Elsayed SM,Phillips JB,Heller R,Thoenes M,Elsobky E,N?rnberg G,N?rnberg P,Seland S,Ebermann I,Altm?ller J,Thiele H,Toliat M,K?rber F,Hu XJ,Wu YD,Zaki MS,Abdel-Salam G,Gleeson J,Boltshauser E,Westerfield M,Bolz HJ

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ahi1 cDNA ORF clone, Danio rerio(zebrafish)

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gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

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