megf6a cDNA ORF clone, Danio rerio(zebrafish)

  • Gene

  • Clones

  • gRNAs

The following megf6a gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the megf6a cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
ODa146261 XM_021473660.1
Latest version!
Danio rerio multiple EGF-like-domains 6a (megf6a), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $797.30
$1139.00
ODa57519 XM_017353739.1
Latest version!
Danio rerio multiple EGF-like-domains 6a (megf6a), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $797.30
$1139.00
ODa146260 XM_021473659.1
Latest version!
Danio rerio multiple EGF-like-domains 6a (megf6a), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $881.30
$1259.00
ODa151555 XM_017353739.2
Latest version!
Danio rerio multiple EGF-like-domains 6a (megf6a), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $881.30
$1259.00
ODa44516 XM_009296882.3
Latest version!
Danio rerio multiple EGF-like-domains 6a (megf6a), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $797.30
$1139.00
View Old Accession Versions >>
ODa44516 XM_009296882.2 Danio rerio multiple EGF-like-domains 6a (megf6a), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $797.30
$1139.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID ODa146261
    Clone ID Related Accession (Same CDS sequence) XM_021473660.1
    Accession Version XM_021473660.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4371bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product multiple epidermal growth factor-like domains protein 6 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_018395144.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    ATGTTATCCG ATTGGTTGAG GTTGGTGCTT GTGTTTACCT TCTGGACTCA GCTGGTACCA 
    AGTTTGGCTG CTGGAAATCC ATATTACCGC TACTATCACA GAAACTGCTA CACCTGCTTC
    AATGGATATC ATGCGTACAA CACAGGACAC CAGGGCGCAG ATATTGATGA GTGCCAGGTT
    CACAACGGAG GCTGCCAGCA CAGATGTGTG AACACTAGAG GCTCGTACTA CTGCGAGTGC
    AATCCAGGCT TTCGGCTCCA CGTTGATGGC CGCACCTGCA TAGCTGTTTT ATCCTGTTCG
    GTAAATAATG GAGGTTGTGA GCATGAATGT GTCCAGCTTT CTCCAGTTCA CTTCCAGTGC
    CGCTGCAGAC GTGGATACCA GCTTACAGCA GATGGCAAGC GCTGTAGATT GAGGAACCCA
    TGTGTGGAGA AGAATGGCGG CTGTGTGCAT TTATGTCGCA GTGATGGCGG TCGGGTTGTG
    TGCAGCTGTC ACCAAGGCTT CATGCTAGCA ATGGACCAGA GGAGCTGTGA AGATGTCAAT
    GAGTGTGAAA CAGGACTGGC AGCATGTGCA CACAGTTGCA GGAACACTCC AGGATCGTTT
    TCCTGCATCT GCAATCCAGG ATACGAGCTG GGCTCTGATG GAAGGAAATG CTACCGTATT
    GAAATGGAGA TTGTCAATAC CTGTGACTCT GATAATGGTG GCTGTTCACA TCGCTGTCAG
    CATGGTGTTC TTGGGCCTGT CTGCAGCTGT AATCATGGTT ATCAGCTCGC CGAAGACCTC
    AAAACCTGTT TGGATCTGGA GGAATGTGAG GAAGGAACGT CATGCTGTGA GCAGGATTGC
    ACAAACTACC CTGGAGGGTA TGAATGCTAC TGCAGGGCAG GTTACAGACT CAATGCAGAT
    GGATGCGGAT GTGACGATGT GGACGAGTGT GCATCTGATA GAGGAGGCTG TGAACACACT
    TGTCAGAATA CGGCTGGCTC ATATCACTGC TTCTGTCGGT TTGGATATCG ACTTGGTGAG
    GACAGACACT CCTGCATTTC TCTCCAGCAA TCGGTTGAGG AATTGCCCGG TCCAGTGGTG
    GTTGAGCGTC CTCTCCTTCA TCTCGCTCTC CTCCAGGATT ACCCACAACC CCTGGAGCGC
    TATCATGACT ATGAGGAGGA CAGTTTTGGA GAGCTGCGGG CAGAGAGCAC CTTCGCTGAG
    AAGTTTGTGT GTTTGAATGG GACGTTTGGC CATGACTGCA GCCTGACATG TGATGATTGC
    TCAAATGGAG GATATTGTAA TGCTGAAGGA GATGCTTGTG ACTGTCCAGA TGGGTGGACG
    GGGATCCTCT GTAACCAAAC CTGTCCAGAG GGAACATTTG GTAAAAACTG CTCCTTCACT
    TGCAAGTGCA GAAACGGGGG CACCTGTGAC CCGATTAGTG GGAAGTGCCG CTGCCCACCT
    GGAGTCAATG GAGACCTGTG CCAGGATGGC TGTCCGAAGG GAATGTATGG AAAGCACTGC
    AACAAGAAAT GTAACTGTGC TAATAACGGC CGCTGCCATC GCACATATGG AGCCTGTCTG
    TGTGATCCAG GACTCTACGG CAGGTTCTGT CACCTCCCTT GCCCCAAGTG GTCGTATGGA
    TCTGGCTGCT CATCCGAGTG TCAGTGTGTG CAACAAAACA CGCTTGAGTG CCATCGACGT
    CATGGAACGT GTGTGTGCAA ACCTGGCTAT CAGGGGAAAA CCTGCAACCG CGAGTGTGAT
    AGTGGATTTT ATGGTCCAGG ATGTAGATTA AAATGCCAGT GTCCAGCGGG AGTCTCTTGC
    CATCCCATGA GTGGGCAATG TCAGCGTCAG TGCCCAGCAG GCCTACATGG GGAGCACTGT
    GACCGAGAGT GCCCCATGGG ACAGTTTGGA GTTGGATGCA CACAGTTTTG TGACTGTAAG
    GGAGCTCCAT GTAACTCAGT GACCGGGAAA TGCCACTGTC CTGCAGGGCA GACAGGACAA
    CACTGTGAAA AAGAGTGTGA AGAGGGCCGT TGGGGTCTTG GCTGTGCAGA ATCATGTCCT
    TCCTGTGACA ATGGTGGAGT ATGTGATAGA CAAAACGGCA CCTGTGTTTG TTCGCCGGGA
    TTCATGGGAA ATCTCTGTCA GAATGTCTGT CCAGATGGGC ACTTTGGCCT GAGCTGCCTG
    TTGCGCTGCT CCTGTCAAAA CGAAGGGAAA TGCCACCATG TGACTGGCCT CTGTGTTTGT
    AAAGACGGCT GGATGGGACC TAACTGCGCA AAAGCTTGTG TGGCTGGACG ATGGGGGCCG
    AACTGTGCCA ACCAGTGTGA CTGCAGGAGC AGCGTGGGCA GCTGTGACCC TGTGATTGGC
    CAGTGTCATT GTGAAGCGGG CTACATGGGA CCACGCTGTG ATCAAAAGTG TCCTAAGGGT
    TTCTTCGGCC AGAGCTGCCA GCACAGATGC CAGTGTGAGA ATGGCGCAGC GTGTGAGCAT
    GTGAGCGGTG CCTGCACTTG TTTGGCTGGC TGGACTGGGA CGTATTGTGA AAAACCATGT
    CCTGAGGGCT TTCATGGCTT GGAGTGCCAG CAGAAGTGCC AGTGCTTGAA TGGTGGCCGT
    TGTCATCCCG TGACTGGAGG CTGCCAGTGT CCATCTGGTT GGGTCGGGCC AGTTTGCAAT
    GCCACGTGTG AAGCTGGCAC CTTTGGGCCG GCCTGCAGTC AAACCTGTAG ATGCCACAAT
    AATGGCACCT GTGACCCTGC TGATGGACGA TGTGAGTGTG GAGCAGGATG GACAGGCAGC
    AGGTGTGAAC AAGAGTGCTC AGCTGGATTG TATGGTGTGG GCTGCAACCA GCATTGCCTG
    TGTCAGAATG GAGGGTCGTG TGACCGAATC AGTGGCCACT GCACATGTGT CAGTGGGTGG
    ACAGGAGTGG CCTGTGAATT GGAATGTGCT GCGGGACACT TTGGCATGGA CTGTCAGCAC
    AAGTGTGAGT GTGTAAATGG CGGCTTGTGT GACCGTCACA GTGGCAGGTG TTTGTGCCCA
    GCAGGTTGGA CAGGCACGCA CTGTGAGACA GCGTGCACTC AAGGTTTTTA TGGTCTGGGC
    TGTAAGGAGC GATGCCGGTG TGATCATAAC GCCGCATGCC ACCACATCAC TGGCACCTGC
    TTGTGTCCCG CTGGCTGGAG AGGGACTCAC TGCGAAAAGG TTTGTTTACC TGGTTCTTAT
    GGGAAGCGTT GTGCTCATCG ATGCCAGTGT CCTCATGGAA CCTCCTGTAA TCATGTGACT
    GGACAGTGTG GTTGCCCAGC GGGATTGACT GGTAGTGGCT GTGAGCGAAT GTGCCAGTCA
    GGTACGTTTG GGGTAAACTG TAATCAGGTG TGCCAGTGCT CTGAGCTCAA CCAGCTCTGC
    CACCCTGTCA CTGGTGTCTG TTACTGTGCT CCCGGATACT ATGGCAGCAA GTGTGAAACA
    GAATGTGAAG CGGGCCGTTA TGGACCCAAC TGCGAGCAGG AATGCCAATG TCTGAATAAC
    GGTGTATGTA AAAGCAGCAC TGGCACCTGC AGCTGCCCAC CGGGATTCAT CGGACCAAAC
    TGCAACATCA CGTGTCCATT AGGGCGTTAT GGTCAGGACT GTTTTGGAGT GTCTCTGTGT
    GGTGAAGGGG CTCGCAGTGA CCCTCTCACA GGCAGATGTG TGTGCAGGGC TGGTCAGCGT
    GGACGGGACT GTGGGAAAGG CTGTGCTGAG GGCTGGTTTG GAGAGAATTG TGATCAGCGC
    TGTAACTGCA GCAATGGAGG AGTGTGTGAC GCTCTTACAG GACACTGCAG CTGTACACTG
    GGCTGGACAG GAGACACCTG TGAGGAAGTG TGTCCCAGAG GACGGTATGG TTCAGACTGC
    AGTGAAGTGT GTGACTGTAA GAATAACAGC ACATGCGACC GAGTGACTGG CGTCTGTCTC
    TGCTCTCCAG GTTTCTACGG ACATCTATGT CAACATGCAT GTCCTGTTGG CTTCCATGGT
    CATCGTTGCC AGCTTCTGTG TGACTGTGGT GCAAATGCAC CTTGTGACCC TGAAACTGGC
    CGATGCCTCT GTCCTGCAGG ATACCAGGGA CTCCGCTGTG AGAGAGAGTG TGAATTGGGC
    ACATTCGGGC CTGATTGTGT CCACAGATGT GACTGTGATG GAGACACCCC ATGTGACCCT
    ATTAATGGCA GGTGTTTGTG TGCACCTGGA AAAACAGGCT CTCGCTGTGA CATAGGCTGT
    GGAGTGAACC GATTTGGACC CGACTGCTCA CAGAGATGTG AATGTCTGAA CGGAGGTCAG
    TGTGATCCCC GAAACGGCCG ATGCACGTGT CTGAACGGCT GGATCGGGCC CAGCTGTCAA
    CAAGGGCTCC TGTCTGCTAG CAAAAGTAAA AGGAAGGACT CGTCAGTATA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_021329335.1
    CDS243..4613
    Translation

    Target ORF information:

    RefSeq Version XM_021473660.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio multiple EGF-like-domains 6a (megf6a), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_021473660.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    ATGTTATCCG ATTGGTTGAG GTTGGTGCTT GTGTTTACCT TCTGGACTCA GCTGGTACCA 
    AGTTTGGCTG CTGGAAATCC ATATTACCGC TACTATCACA GAAACTGCTA CACCTGCTTC
    AATGGATATC ATGCGTACAA CACAGGACAC CAGGGCGCAG ATATTGATGA GTGCCAGGTT
    CACAACGGAG GCTGCCAGCA CAGATGTGTG AACACTAGAG GCTCGTACTA CTGCGAGTGC
    AATCCAGGCT TTCGGCTCCA CGTTGATGGC CGCACCTGCA TAGCTGTTTT ATCCTGTTCG
    GTAAATAATG GAGGTTGTGA GCATGAATGT GTCCAGCTTT CTCCAGTTCA CTTCCAGTGC
    CGCTGCAGAC GTGGATACCA GCTTACAGCA GATGGCAAGC GCTGTAGATT GAGGAACCCA
    TGTGTGGAGA AGAATGGCGG CTGTGTGCAT TTATGTCGCA GTGATGGCGG TCGGGTTGTG
    TGCAGCTGTC ACCAAGGCTT CATGCTAGCA ATGGACCAGA GGAGCTGTGA AGATGTCAAT
    GAGTGTGAAA CAGGACTGGC AGCATGTGCA CACAGTTGCA GGAACACTCC AGGATCGTTT
    TCCTGCATCT GCAATCCAGG ATACGAGCTG GGCTCTGATG GAAGGAAATG CTACCGTATT
    GAAATGGAGA TTGTCAATAC CTGTGACTCT GATAATGGTG GCTGTTCACA TCGCTGTCAG
    CATGGTGTTC TTGGGCCTGT CTGCAGCTGT AATCATGGTT ATCAGCTCGC CGAAGACCTC
    AAAACCTGTT TGGATCTGGA GGAATGTGAG GAAGGAACGT CATGCTGTGA GCAGGATTGC
    ACAAACTACC CTGGAGGGTA TGAATGCTAC TGCAGGGCAG GTTACAGACT CAATGCAGAT
    GGATGCGGAT GTGACGATGT GGACGAGTGT GCATCTGATA GAGGAGGCTG TGAACACACT
    TGTCAGAATA CGGCTGGCTC ATATCACTGC TTCTGTCGGT TTGGATATCG ACTTGGTGAG
    GACAGACACT CCTGCATTTC TCTCCAGCAA TCGGTTGAGG AATTGCCCGG TCCAGTGGTG
    GTTGAGCGTC CTCTCCTTCA TCTCGCTCTC CTCCAGGATT ACCCACAACC CCTGGAGCGC
    TATCATGACT ATGAGGAGGA CAGTTTTGGA GAGCTGCGGG CAGAGAGCAC CTTCGCTGAG
    AAGTTTGTGT GTTTGAATGG GACGTTTGGC CATGACTGCA GCCTGACATG TGATGATTGC
    TCAAATGGAG GATATTGTAA TGCTGAAGGA GATGCTTGTG ACTGTCCAGA TGGGTGGACG
    GGGATCCTCT GTAACCAAAC CTGTCCAGAG GGAACATTTG GTAAAAACTG CTCCTTCACT
    TGCAAGTGCA GAAACGGGGG CACCTGTGAC CCGATTAGTG GGAAGTGCCG CTGCCCACCT
    GGAGTCAATG GAGACCTGTG CCAGGATGGC TGTCCGAAGG GAATGTATGG AAAGCACTGC
    AACAAGAAAT GTAACTGTGC TAATAACGGC CGCTGCCATC GCACATATGG AGCCTGTCTG
    TGTGATCCAG GACTCTACGG CAGGTTCTGT CACCTCCCTT GCCCCAAGTG GTCGTATGGA
    TCTGGCTGCT CATCCGAGTG TCAGTGTGTG CAACAAAACA CGCTTGAGTG CCATCGACGT
    CATGGAACGT GTGTGTGCAA ACCTGGCTAT CAGGGGAAAA CCTGCAACCG CGAGTGTGAT
    AGTGGATTTT ATGGTCCAGG ATGTAGATTA AAATGCCAGT GTCCAGCGGG AGTCTCTTGC
    CATCCCATGA GTGGGCAATG TCAGCGTCAG TGCCCAGCAG GCCTACATGG GGAGCACTGT
    GACCGAGAGT GCCCCATGGG ACAGTTTGGA GTTGGATGCA CACAGTTTTG TGACTGTAAG
    GGAGCTCCAT GTAACTCAGT GACCGGGAAA TGCCACTGTC CTGCAGGGCA GACAGGACAA
    CACTGTGAAA AAGAGTGTGA AGAGGGCCGT TGGGGTCTTG GCTGTGCAGA ATCATGTCCT
    TCCTGTGACA ATGGTGGAGT ATGTGATAGA CAAAACGGCA CCTGTGTTTG TTCGCCGGGA
    TTCATGGGAA ATCTCTGTCA GAATGTCTGT CCAGATGGGC ACTTTGGCCT GAGCTGCCTG
    TTGCGCTGCT CCTGTCAAAA CGAAGGGAAA TGCCACCATG TGACTGGCCT CTGTGTTTGT
    AAAGACGGCT GGATGGGACC TAACTGCGCA AAAGCTTGTG TGGCTGGACG ATGGGGGCCG
    AACTGTGCCA ACCAGTGTGA CTGCAGGAGC AGCGTGGGCA GCTGTGACCC TGTGATTGGC
    CAGTGTCATT GTGAAGCGGG CTACATGGGA CCACGCTGTG ATCAAAAGTG TCCTAAGGGT
    TTCTTCGGCC AGAGCTGCCA GCACAGATGC CAGTGTGAGA ATGGCGCAGC GTGTGAGCAT
    GTGAGCGGTG CCTGCACTTG TTTGGCTGGC TGGACTGGGA CGTATTGTGA AAAACCATGT
    CCTGAGGGCT TTCATGGCTT GGAGTGCCAG CAGAAGTGCC AGTGCTTGAA TGGTGGCCGT
    TGTCATCCCG TGACTGGAGG CTGCCAGTGT CCATCTGGTT GGGTCGGGCC AGTTTGCAAT
    GCCACGTGTG AAGCTGGCAC CTTTGGGCCG GCCTGCAGTC AAACCTGTAG ATGCCACAAT
    AATGGCACCT GTGACCCTGC TGATGGACGA TGTGAGTGTG GAGCAGGATG GACAGGCAGC
    AGGTGTGAAC AAGAGTGCTC AGCTGGATTG TATGGTGTGG GCTGCAACCA GCATTGCCTG
    TGTCAGAATG GAGGGTCGTG TGACCGAATC AGTGGCCACT GCACATGTGT CAGTGGGTGG
    ACAGGAGTGG CCTGTGAATT GGAATGTGCT GCGGGACACT TTGGCATGGA CTGTCAGCAC
    AAGTGTGAGT GTGTAAATGG CGGCTTGTGT GACCGTCACA GTGGCAGGTG TTTGTGCCCA
    GCAGGTTGGA CAGGCACGCA CTGTGAGACA GCGTGCACTC AAGGTTTTTA TGGTCTGGGC
    TGTAAGGAGC GATGCCGGTG TGATCATAAC GCCGCATGCC ACCACATCAC TGGCACCTGC
    TTGTGTCCCG CTGGCTGGAG AGGGACTCAC TGCGAAAAGG TTTGTTTACC TGGTTCTTAT
    GGGAAGCGTT GTGCTCATCG ATGCCAGTGT CCTCATGGAA CCTCCTGTAA TCATGTGACT
    GGACAGTGTG GTTGCCCAGC GGGATTGACT GGTAGTGGCT GTGAGCGAAT GTGCCAGTCA
    GGTACGTTTG GGGTAAACTG TAATCAGGTG TGCCAGTGCT CTGAGCTCAA CCAGCTCTGC
    CACCCTGTCA CTGGTGTCTG TTACTGTGCT CCCGGATACT ATGGCAGCAA GTGTGAAACA
    GAATGTGAAG CGGGCCGTTA TGGACCCAAC TGCGAGCAGG AATGCCAATG TCTGAATAAC
    GGTGTATGTA AAAGCAGCAC TGGCACCTGC AGCTGCCCAC CGGGATTCAT CGGACCAAAC
    TGCAACATCA CGTGTCCATT AGGGCGTTAT GGTCAGGACT GTTTTGGAGT GTCTCTGTGT
    GGTGAAGGGG CTCGCAGTGA CCCTCTCACA GGCAGATGTG TGTGCAGGGC TGGTCAGCGT
    GGACGGGACT GTGGGAAAGG CTGTGCTGAG GGCTGGTTTG GAGAGAATTG TGATCAGCGC
    TGTAACTGCA GCAATGGAGG AGTGTGTGAC GCTCTTACAG GACACTGCAG CTGTACACTG
    GGCTGGACAG GAGACACCTG TGAGGAAGTG TGTCCCAGAG GACGGTATGG TTCAGACTGC
    AGTGAAGTGT GTGACTGTAA GAATAACAGC ACATGCGACC GAGTGACTGG CGTCTGTCTC
    TGCTCTCCAG GTTTCTACGG ACATCTATGT CAACATGCAT GTCCTGTTGG CTTCCATGGT
    CATCGTTGCC AGCTTCTGTG TGACTGTGGT GCAAATGCAC CTTGTGACCC TGAAACTGGC
    CGATGCCTCT GTCCTGCAGG ATACCAGGGA CTCCGCTGTG AGAGAGAGTG TGAATTGGGC
    ACATTCGGGC CTGATTGTGT CCACAGATGT GACTGTGATG GAGACACCCC ATGTGACCCT
    ATTAATGGCA GGTGTTTGTG TGCACCTGGA AAAACAGGCT CTCGCTGTGA CATAGGCTGT
    GGAGTGAACC GATTTGGACC CGACTGCTCA CAGAGATGTG AATGTCTGAA CGGAGGTCAG
    TGTGATCCCC GAAACGGCCG ATGCACGTGT CTGAACGGCT GGATCGGGCC CAGCTGTCAA
    CAAGGGCTCC TGTCTGCTAG CAAAAGTAAA AGGAAGGACT CGTCAGTATA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa57519
    Clone ID Related Accession (Same CDS sequence) XM_017353739.1
    Accession Version XM_017353739.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4383bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product multiple epidermal growth factor-like domains protein 6 isoform X1
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007134.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    ATGGTGAAGG TCTGGAGGCA AGGATGCACA GGTCATGCTT GGTGTATGGA CTTCGAGAGG 
    AGGACTACCT ACTACATAGG ATATAAACAA GTCTACAGGC AGGACTTCAA GATGGCATAC
    AAATGCTGTC CTGGATGGTC CCAGAACAAT TCAAAGATGG GCTGCCTTTA TCATATTGAT
    GAGTGCCAGG TTCACAACGG AGGCTGCCAG CACAGATGTG TGAACACTAG AGGCTCGTAC
    TACTGCGAGT GCAATCCAGG CTTTCGGCTC CACGTTGATG GCCGCACCTG CATAGCTGTT
    TTATCCTGTT CGGTAAATAA TGGAGGTTGT GAGCATGAAT GTGTCCAGCT TTCTCCAGTT
    CACTTCCAGT GCCGCTGCAG ACGTGGATAC CAGCTTACAG CAGATGGCAA GCGCTGTAGA
    TTGAGGAACC CATGTGTGGA GAAGAATGGC GGCTGTGTGC ATTTATGTCG CAGTGATGGT
    GGTCGGGTTG TGTGCAGCTG TCACCAAGGC TTCATGCTAG CAGTGGACCA GAAGAGCTGT
    GAAGATGTCA ATGAGTGTGA AACAGGACTG GCAGCATGTG CACACAGTTG CAGGAACACT
    CCAGGATCGT TTTCCTGCAT CTGCAATCCA GGATACGAGC TGGGCTCTGA TGGAAGGAAA
    TGCTACCGTA TTGAAATGGA GATTGTCAAT ACCTGTGACT CTGATAATGG TGGCTGTTCA
    CATCGCTGTC AGCATGGTGT TCTTGGGCCT GTCTGCAGCT GTAATCATGG TTATCAGCTC
    GCCGAAGACC TCAAAACCTG TTTGGATCTG GAGGAATGTG AGGAAGGAAC GTCATGCTGT
    GAGCAGGATT GCACAAACTA CCCTGGAGGG TATGAATGCT ACTGCAGGGC AGGTTACAGA
    CTCAATGCAG ATGGATGCGG ATGTGACGAT GTGGACGAGT GTGCGTCTGA TAGAGGAGGC
    TGTGAACACA CTTGTCAGAA TACGGCTGGC TCATATCACT GCTTCTGTCG GTTTGGATAT
    CGACTTGGTG AGGACAGACA CTCCTGCATT TCTCTCCAGC GATCGGTTGA GGAATTGCCC
    GGTCCAGTGG TGGTTGAGCG TCCTCTCCTT CATCTCGCTC TCCTCCAGGA TTACCCACAA
    CCCCTGGAGC GCTATCATGA CTATGAGGAG GACAGTTTTG GAGAGCTGCG GGCAGAGAGC
    ACCTTCGCTG AGAAGTTTGT GTGTTTGAAT GGGACGTTTG GCCATGACTG CAGCCTGACG
    TGTGATGATT GCTCAAATGG AGGATATTGT AATGCTGAAG GAGATGCTTG TGACTGTCCA
    GATGGGTGGA CGGGGATCCT TTGTAACCAA ACCTGTCCAG AGGGAACATT TGGTAAAAAC
    TGCTCCTTCA CTTGCAAGTG CAGAAACGGG GGCACCTGTG ACCCGATTAG TGGGAAGTGC
    CGCTGCCCAC CTGGAGTCAA TGGAGACCTG TGCCAGGACG GCTGTCCGAA GGGAATGTAT
    GGAAAGCACT GCAACAAGAA ATGTAACTGT GCTAATAACG GCCGCTGCCA TCGCACATAT
    GGAGCCTGTC TGTGTGATCC AGGACTCTAC GGCAGGTTCT GTCACCTCCC TTGCCCCAAG
    TGGTCGTATG GATCTGGCTG CTCATCCGAG TGTCAGTGTG TGCAACAAAA CACGCTTGAG
    TGCCATCGAC GTCATGGAAC GTGTGTGTGC AAACCTGGCT ATCAGGGGAA AACCTGCAAC
    CGCGAGTGTG ATAGTGGATT TTATGGTCCA GGATGTAGAT TAAAATGCCA GTGTCCAGCG
    GGAGTCTCTT GCCATCCCAT GAGTGGGCAA TGTCAGCGTC AGTGCCCAGC AGGCCTACAT
    GGGGAGCACT GTGACCGAGA GTGCCCCATG GGACAGTTTG GAGTTGGATG CACACAGTTT
    TGTGACTGTA AGGGAGCTCC ATGTAACTCA GTGACCGGGA AATGCCACTG TCCTGCAGGG
    CAGACAGGAC AACACTGTGA AAAAGAGTGT GAAGAGGGCC GTTGGGGTCT TGGCTGTGCA
    GAATCATGTC CTTCCTGTGA CAATGGTGGA GTATGTGATA GACAAAACGG CACCTGTGTT
    TGTTCGCCGG GATTCATGGG AAATCTCTGT CAGAATGTCT GTCCAGATGG GCACTTTGGC
    CTGAGCTGCC TGTTGCGCTG CTCCTGTCAA AACGAAGGGA AATGCCACCA TGTGACTGGC
    CTCTGTGTTT GTAAAGATGG CTGGATGGGA CCCAACTGCG CAAAAGCTTG TGTGGCTGGA
    CGATGGGGGC CGAACTGTGC CAACCAGTGT GACTGCAGGA GCAGCGTGGG CAGCTGTGAC
    CCTGTGATTG GCCTGTGTCA TTGTGAAGCG GGCTACATGG GACCACGCTG TGATCAAAAG
    TGTCCTCAGG GTTTCTTCGG CCAGAGCTGC CAGCACAGAT GCCAGTGTGA GAATGGCGCA
    GTGTGTGAGC ATGTGAGCGG TGCCTGCACT TGTTTGGCTG GCTGGACTGG GACGTATTGT
    GAAAAACCTT GTCCTGAGGG CTTTCATGGC TTGGAGTGCC AGCAGAAGTG CCAGTGCTTG
    AATGGTGGCC GTTGTCATCC CGTGACTGGA GGCTGCCAGT GTCCATCTGG TTGGGTCGGG
    CCAGTTTGCA ATGCCACGTG TGAAGCTGGC ACCTTTGGGC CGGCCTGCAG TCAAACCTGT
    AGATGCCACA ATAATGGCAC CTGTGACCCT GCTGATGGGC GATGTGAGTG TGGAGCAGGA
    TGGACAGGCA GCAGCTGTGA ACAAGAGTGC TCAGCTGGAT TGTATGGTGT GGGCTGCAAC
    CAGCATTGCC TGTGTCAGAA TGGAGGGTCG TGTGACCGAA TCAGTGGCCA CTGCACATGT
    GTCAGTGGGT GGACAGGAGT GGCCTGTGAA TTGGAATGTG CTGCGGGACA CTTTGGCATG
    GACTGTCAGC ACAAGTGTGA GTGTGTAAAT GGCGGCGTGT GTGACCGTCA CAGTGGCAGG
    TGTTTGTGCC CAGCAGGTTG GACAGGCACG CACTGTGAGA CAGCATGCAC TCAAGGTTTT
    TATGGTCCGG GCTGTAAGGA GCGATGCCGG TGTGATCATA ACGCCGCATG CCACCACATC
    ACTGGCACCT GCTTGTGTCC CGCTGGCTGG AGAGGGACTC ACTGCGAAAA GGTTTGTTTA
    CCTGGTTCCT ATGGGAAGCG TTGTGCTCAT CGATGCCAGT GTCCTCATGG AACCTCCTGT
    AACCATGTGA CTGGACAGTG TGGTTGCCCA GCGGGATTAA CTGGTAGTGG CTGTGAGCGA
    ATGTGCCAGT CAGGTACGTT TGGGGTAAAC TGTAATCAGG TGTGCCAGTG CTCTGAGCTC
    AACCAGCTCT GCCACCCCGT CACTGGTGTC TGTTACTGTG CTCCCGGATA CTATGGCAGC
    AAGTGTGAAA CAGAATGTGA AGCGGGCCGT TATGGACCCA ACTGTGAGCA GGAATGCCAG
    TGTCTGAATA ACGGTGTATG TAAAAGCAGC ACTGGCACCT GCAGCTGCCC ACCGGGATTC
    ATCGGACCAA ACTGCAACAT CACATGTCCA TTAGGGCGTT ATGGTCAGGA CTGTGCTGGA
    GTGTCTCTGT GTGGTGAAGG GGCTCGCAGT GACCCTCTCA CAGGCAGATG TGTGTGCAGG
    GCTGGTCAGC GTGGACGGGA CTGTGGGAAA GGCTGTGCTG AGGGCTGGTT TGGAGAGAAT
    TGTGATCAGC GCTGTAACTG CAGCAATGGC GGAGTGTGTG ACGCTCTTAC AGGACACTGC
    AGCTGTACAC TGGGCTGGAC AGGAGACACC TGTGAGGAAG TGTGTCCCAG AGGACGGTAT
    GGTTCAGACT GCAGTGAAGT GTGTGACTGT AAGAATAACA GCACATGCGA CCGAGTGACT
    GGCGTCTGTC TCTGCTCTCC AGGTTTCTAC GGACATCTAT GTCAACATGC ATGTCCTGTT
    GGCTTCCATG GTCATCGTTG CCAGCTTCTG TGTGACTGTG GTGCAAATGC ACCTTGTGAC
    CCTGAAACTG GCCGATGCCT CTGTCCTGCA GGATACCAGG GACTCCGCTG TGAGAGAGAG
    TGTGAATTGG GCACATTTGG GGCTGATTGT GTCCACAGAT GTGACTGTGA TGGAGACACC
    CCATGTGACC CTATTAATGG CAGGTGTTTG TGTGCACCTG GAAAAACAGG CTCTCGCTGT
    GACATAGGCT GTGGAGTGAA CCGATTTGGA CCCGACTGCT CACAGAGATG TGAATGTCTG
    AACGGAGGTC AGTGTGATCC CCGAAACGGC CGATGCACGT GTCTGAACGG CTGGATCGGG
    CCCAGCTGTC AACAAGGGCT CCTGTCTGCT AGCAAAAGTA AAAGGAAGGA CTCGTCAGTA
    TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017209228.1
    CDS57..4439
    Translation

    Target ORF information:

    RefSeq Version XM_017353739.1
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio multiple EGF-like-domains 6a (megf6a), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017353739.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    ATGGTGAAGG TCTGGAGGCA AGGATGCACA GGTCATGCTT GGTGTATGGA CTTCGAGAGG 
    AGGACTACCT ACTACATAGG ATATAAACAA GTCTACAGGC AGGACTTCAA GATGGCATAC
    AAATGCTGTC CTGGATGGTC CCAGAACAAT TCAAAGATGG GCTGCCTTTA TCATATTGAT
    GAGTGCCAGG TTCACAACGG AGGCTGCCAG CACAGATGTG TGAACACTAG AGGCTCGTAC
    TACTGCGAGT GCAATCCAGG CTTTCGGCTC CACGTTGATG GCCGCACCTG CATAGCTGTT
    TTATCCTGTT CGGTAAATAA TGGAGGTTGT GAGCATGAAT GTGTCCAGCT TTCTCCAGTT
    CACTTCCAGT GCCGCTGCAG ACGTGGATAC CAGCTTACAG CAGATGGCAA GCGCTGTAGA
    TTGAGGAACC CATGTGTGGA GAAGAATGGC GGCTGTGTGC ATTTATGTCG CAGTGATGGT
    GGTCGGGTTG TGTGCAGCTG TCACCAAGGC TTCATGCTAG CAGTGGACCA GAAGAGCTGT
    GAAGATGTCA ATGAGTGTGA AACAGGACTG GCAGCATGTG CACACAGTTG CAGGAACACT
    CCAGGATCGT TTTCCTGCAT CTGCAATCCA GGATACGAGC TGGGCTCTGA TGGAAGGAAA
    TGCTACCGTA TTGAAATGGA GATTGTCAAT ACCTGTGACT CTGATAATGG TGGCTGTTCA
    CATCGCTGTC AGCATGGTGT TCTTGGGCCT GTCTGCAGCT GTAATCATGG TTATCAGCTC
    GCCGAAGACC TCAAAACCTG TTTGGATCTG GAGGAATGTG AGGAAGGAAC GTCATGCTGT
    GAGCAGGATT GCACAAACTA CCCTGGAGGG TATGAATGCT ACTGCAGGGC AGGTTACAGA
    CTCAATGCAG ATGGATGCGG ATGTGACGAT GTGGACGAGT GTGCGTCTGA TAGAGGAGGC
    TGTGAACACA CTTGTCAGAA TACGGCTGGC TCATATCACT GCTTCTGTCG GTTTGGATAT
    CGACTTGGTG AGGACAGACA CTCCTGCATT TCTCTCCAGC GATCGGTTGA GGAATTGCCC
    GGTCCAGTGG TGGTTGAGCG TCCTCTCCTT CATCTCGCTC TCCTCCAGGA TTACCCACAA
    CCCCTGGAGC GCTATCATGA CTATGAGGAG GACAGTTTTG GAGAGCTGCG GGCAGAGAGC
    ACCTTCGCTG AGAAGTTTGT GTGTTTGAAT GGGACGTTTG GCCATGACTG CAGCCTGACG
    TGTGATGATT GCTCAAATGG AGGATATTGT AATGCTGAAG GAGATGCTTG TGACTGTCCA
    GATGGGTGGA CGGGGATCCT TTGTAACCAA ACCTGTCCAG AGGGAACATT TGGTAAAAAC
    TGCTCCTTCA CTTGCAAGTG CAGAAACGGG GGCACCTGTG ACCCGATTAG TGGGAAGTGC
    CGCTGCCCAC CTGGAGTCAA TGGAGACCTG TGCCAGGACG GCTGTCCGAA GGGAATGTAT
    GGAAAGCACT GCAACAAGAA ATGTAACTGT GCTAATAACG GCCGCTGCCA TCGCACATAT
    GGAGCCTGTC TGTGTGATCC AGGACTCTAC GGCAGGTTCT GTCACCTCCC TTGCCCCAAG
    TGGTCGTATG GATCTGGCTG CTCATCCGAG TGTCAGTGTG TGCAACAAAA CACGCTTGAG
    TGCCATCGAC GTCATGGAAC GTGTGTGTGC AAACCTGGCT ATCAGGGGAA AACCTGCAAC
    CGCGAGTGTG ATAGTGGATT TTATGGTCCA GGATGTAGAT TAAAATGCCA GTGTCCAGCG
    GGAGTCTCTT GCCATCCCAT GAGTGGGCAA TGTCAGCGTC AGTGCCCAGC AGGCCTACAT
    GGGGAGCACT GTGACCGAGA GTGCCCCATG GGACAGTTTG GAGTTGGATG CACACAGTTT
    TGTGACTGTA AGGGAGCTCC ATGTAACTCA GTGACCGGGA AATGCCACTG TCCTGCAGGG
    CAGACAGGAC AACACTGTGA AAAAGAGTGT GAAGAGGGCC GTTGGGGTCT TGGCTGTGCA
    GAATCATGTC CTTCCTGTGA CAATGGTGGA GTATGTGATA GACAAAACGG CACCTGTGTT
    TGTTCGCCGG GATTCATGGG AAATCTCTGT CAGAATGTCT GTCCAGATGG GCACTTTGGC
    CTGAGCTGCC TGTTGCGCTG CTCCTGTCAA AACGAAGGGA AATGCCACCA TGTGACTGGC
    CTCTGTGTTT GTAAAGATGG CTGGATGGGA CCCAACTGCG CAAAAGCTTG TGTGGCTGGA
    CGATGGGGGC CGAACTGTGC CAACCAGTGT GACTGCAGGA GCAGCGTGGG CAGCTGTGAC
    CCTGTGATTG GCCTGTGTCA TTGTGAAGCG GGCTACATGG GACCACGCTG TGATCAAAAG
    TGTCCTCAGG GTTTCTTCGG CCAGAGCTGC CAGCACAGAT GCCAGTGTGA GAATGGCGCA
    GTGTGTGAGC ATGTGAGCGG TGCCTGCACT TGTTTGGCTG GCTGGACTGG GACGTATTGT
    GAAAAACCTT GTCCTGAGGG CTTTCATGGC TTGGAGTGCC AGCAGAAGTG CCAGTGCTTG
    AATGGTGGCC GTTGTCATCC CGTGACTGGA GGCTGCCAGT GTCCATCTGG TTGGGTCGGG
    CCAGTTTGCA ATGCCACGTG TGAAGCTGGC ACCTTTGGGC CGGCCTGCAG TCAAACCTGT
    AGATGCCACA ATAATGGCAC CTGTGACCCT GCTGATGGGC GATGTGAGTG TGGAGCAGGA
    TGGACAGGCA GCAGCTGTGA ACAAGAGTGC TCAGCTGGAT TGTATGGTGT GGGCTGCAAC
    CAGCATTGCC TGTGTCAGAA TGGAGGGTCG TGTGACCGAA TCAGTGGCCA CTGCACATGT
    GTCAGTGGGT GGACAGGAGT GGCCTGTGAA TTGGAATGTG CTGCGGGACA CTTTGGCATG
    GACTGTCAGC ACAAGTGTGA GTGTGTAAAT GGCGGCGTGT GTGACCGTCA CAGTGGCAGG
    TGTTTGTGCC CAGCAGGTTG GACAGGCACG CACTGTGAGA CAGCATGCAC TCAAGGTTTT
    TATGGTCCGG GCTGTAAGGA GCGATGCCGG TGTGATCATA ACGCCGCATG CCACCACATC
    ACTGGCACCT GCTTGTGTCC CGCTGGCTGG AGAGGGACTC ACTGCGAAAA GGTTTGTTTA
    CCTGGTTCCT ATGGGAAGCG TTGTGCTCAT CGATGCCAGT GTCCTCATGG AACCTCCTGT
    AACCATGTGA CTGGACAGTG TGGTTGCCCA GCGGGATTAA CTGGTAGTGG CTGTGAGCGA
    ATGTGCCAGT CAGGTACGTT TGGGGTAAAC TGTAATCAGG TGTGCCAGTG CTCTGAGCTC
    AACCAGCTCT GCCACCCCGT CACTGGTGTC TGTTACTGTG CTCCCGGATA CTATGGCAGC
    AAGTGTGAAA CAGAATGTGA AGCGGGCCGT TATGGACCCA ACTGTGAGCA GGAATGCCAG
    TGTCTGAATA ACGGTGTATG TAAAAGCAGC ACTGGCACCT GCAGCTGCCC ACCGGGATTC
    ATCGGACCAA ACTGCAACAT CACATGTCCA TTAGGGCGTT ATGGTCAGGA CTGTGCTGGA
    GTGTCTCTGT GTGGTGAAGG GGCTCGCAGT GACCCTCTCA CAGGCAGATG TGTGTGCAGG
    GCTGGTCAGC GTGGACGGGA CTGTGGGAAA GGCTGTGCTG AGGGCTGGTT TGGAGAGAAT
    TGTGATCAGC GCTGTAACTG CAGCAATGGC GGAGTGTGTG ACGCTCTTAC AGGACACTGC
    AGCTGTACAC TGGGCTGGAC AGGAGACACC TGTGAGGAAG TGTGTCCCAG AGGACGGTAT
    GGTTCAGACT GCAGTGAAGT GTGTGACTGT AAGAATAACA GCACATGCGA CCGAGTGACT
    GGCGTCTGTC TCTGCTCTCC AGGTTTCTAC GGACATCTAT GTCAACATGC ATGTCCTGTT
    GGCTTCCATG GTCATCGTTG CCAGCTTCTG TGTGACTGTG GTGCAAATGC ACCTTGTGAC
    CCTGAAACTG GCCGATGCCT CTGTCCTGCA GGATACCAGG GACTCCGCTG TGAGAGAGAG
    TGTGAATTGG GCACATTTGG GGCTGATTGT GTCCACAGAT GTGACTGTGA TGGAGACACC
    CCATGTGACC CTATTAATGG CAGGTGTTTG TGTGCACCTG GAAAAACAGG CTCTCGCTGT
    GACATAGGCT GTGGAGTGAA CCGATTTGGA CCCGACTGCT CACAGAGATG TGAATGTCTG
    AACGGAGGTC AGTGTGATCC CCGAAACGGC CGATGCACGT GTCTGAACGG CTGGATCGGG
    CCCAGCTGTC AACAAGGGCT CCTGTCTGCT AGCAAAAGTA AAAGGAAGGA CTCGTCAGTA
    TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa146260
    Clone ID Related Accession (Same CDS sequence) XM_021473659.1
    Accession Version XM_021473659.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4542bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product multiple epidermal growth factor-like domains protein 6 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_018395144.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    ATGGATTTAC ACGTGTTGTT GTTATTATTA TTCCTGCTAA CCAAGATCCG TCCATCGTGT 
    TGTCTTCGAG CTCTACATCC TTTCATGCCG AATGTTTGCT CAGAGCCAGA GAGAGTCATC
    CGAGATGAGA AACAGCCATG TGTGCAGGCA TTTATACAAA TGGTGAAGGT CTGGAGGCAA
    GGATGCACAG GTCATGCTTG GTGTATGGAC TTCGAGAGGA GGACTACCTA CTACATAGGA
    TATAAACAAG TCTACAGGCA GGACTTCAAG ATGGCATACA AATGCTGTCC TGGATGGTCC
    CAGAACAATT CAAAGATGGG CTGCCTTTAT CATATTGATG AGTGCCAGGT TCACAACGGA
    GGCTGCCAGC ACAGATGTGT GAACACTAGA GGCTCGTACT ACTGCGAGTG CAATCCAGGC
    TTTCGGCTCC ACGTTGATGG CCGCACCTGC ATAGCTGTTT TATCCTGTTC GGTAAATAAT
    GGAGGTTGTG AGCATGAATG TGTCCAGCTT TCTCCAGTTC ACTTCCAGTG CCGCTGCAGA
    CGTGGATACC AGCTTACAGC AGATGGCAAG CGCTGTAGAT TGAGGAACCC ATGTGTGGAG
    AAGAATGGCG GCTGTGTGCA TTTATGTCGC AGTGATGGCG GTCGGGTTGT GTGCAGCTGT
    CACCAAGGCT TCATGCTAGC AATGGACCAG AGGAGCTGTG AAGATGTCAA TGAGTGTGAA
    ACAGGACTGG CAGCATGTGC ACACAGTTGC AGGAACACTC CAGGATCGTT TTCCTGCATC
    TGCAATCCAG GATACGAGCT GGGCTCTGAT GGAAGGAAAT GCTACCGTAT TGAAATGGAG
    ATTGTCAATA CCTGTGACTC TGATAATGGT GGCTGTTCAC ATCGCTGTCA GCATGGTGTT
    CTTGGGCCTG TCTGCAGCTG TAATCATGGT TATCAGCTCG CCGAAGACCT CAAAACCTGT
    TTGGATCTGG AGGAATGTGA GGAAGGAACG TCATGCTGTG AGCAGGATTG CACAAACTAC
    CCTGGAGGGT ATGAATGCTA CTGCAGGGCA GGTTACAGAC TCAATGCAGA TGGATGCGGA
    TGTGACGATG TGGACGAGTG TGCATCTGAT AGAGGAGGCT GTGAACACAC TTGTCAGAAT
    ACGGCTGGCT CATATCACTG CTTCTGTCGG TTTGGATATC GACTTGGTGA GGACAGACAC
    TCCTGCATTT CTCTCCAGCA ATCGGTTGAG GAATTGCCCG GTCCAGTGGT GGTTGAGCGT
    CCTCTCCTTC ATCTCGCTCT CCTCCAGGAT TACCCACAAC CCCTGGAGCG CTATCATGAC
    TATGAGGAGG ACAGTTTTGG AGAGCTGCGG GCAGAGAGCA CCTTCGCTGA GAAGTTTGTG
    TGTTTGAATG GGACGTTTGG CCATGACTGC AGCCTGACAT GTGATGATTG CTCAAATGGA
    GGATATTGTA ATGCTGAAGG AGATGCTTGT GACTGTCCAG ATGGGTGGAC GGGGATCCTC
    TGTAACCAAA CCTGTCCAGA GGGAACATTT GGTAAAAACT GCTCCTTCAC TTGCAAGTGC
    AGAAACGGGG GCACCTGTGA CCCGATTAGT GGGAAGTGCC GCTGCCCACC TGGAGTCAAT
    GGAGACCTGT GCCAGGATGG CTGTCCGAAG GGAATGTATG GAAAGCACTG CAACAAGAAA
    TGTAACTGTG CTAATAACGG CCGCTGCCAT CGCACATATG GAGCCTGTCT GTGTGATCCA
    GGACTCTACG GCAGGTTCTG TCACCTCCCT TGCCCCAAGT GGTCGTATGG ATCTGGCTGC
    TCATCCGAGT GTCAGTGTGT GCAACAAAAC ACGCTTGAGT GCCATCGACG TCATGGAACG
    TGTGTGTGCA AACCTGGCTA TCAGGGGAAA ACCTGCAACC GCGAGTGTGA TAGTGGATTT
    TATGGTCCAG GATGTAGATT AAAATGCCAG TGTCCAGCGG GAGTCTCTTG CCATCCCATG
    AGTGGGCAAT GTCAGCGTCA GTGCCCAGCA GGCCTACATG GGGAGCACTG TGACCGAGAG
    TGCCCCATGG GACAGTTTGG AGTTGGATGC ACACAGTTTT GTGACTGTAA GGGAGCTCCA
    TGTAACTCAG TGACCGGGAA ATGCCACTGT CCTGCAGGGC AGACAGGACA ACACTGTGAA
    AAAGAGTGTG AAGAGGGCCG TTGGGGTCTT GGCTGTGCAG AATCATGTCC TTCCTGTGAC
    AATGGTGGAG TATGTGATAG ACAAAACGGC ACCTGTGTTT GTTCGCCGGG ATTCATGGGA
    AATCTCTGTC AGAATGTCTG TCCAGATGGG CACTTTGGCC TGAGCTGCCT GTTGCGCTGC
    TCCTGTCAAA ACGAAGGGAA ATGCCACCAT GTGACTGGCC TCTGTGTTTG TAAAGACGGC
    TGGATGGGAC CTAACTGCGC AAAAGCTTGT GTGGCTGGAC GATGGGGGCC GAACTGTGCC
    AACCAGTGTG ACTGCAGGAG CAGCGTGGGC AGCTGTGACC CTGTGATTGG CCAGTGTCAT
    TGTGAAGCGG GCTACATGGG ACCACGCTGT GATCAAAAGT GTCCTAAGGG TTTCTTCGGC
    CAGAGCTGCC AGCACAGATG CCAGTGTGAG AATGGCGCAG CGTGTGAGCA TGTGAGCGGT
    GCCTGCACTT GTTTGGCTGG CTGGACTGGG ACGTATTGTG AAAAACCATG TCCTGAGGGC
    TTTCATGGCT TGGAGTGCCA GCAGAAGTGC CAGTGCTTGA ATGGTGGCCG TTGTCATCCC
    GTGACTGGAG GCTGCCAGTG TCCATCTGGT TGGGTCGGGC CAGTTTGCAA TGCCACGTGT
    GAAGCTGGCA CCTTTGGGCC GGCCTGCAGT CAAACCTGTA GATGCCACAA TAATGGCACC
    TGTGACCCTG CTGATGGACG ATGTGAGTGT GGAGCAGGAT GGACAGGCAG CAGGTGTGAA
    CAAGAGTGCT CAGCTGGATT GTATGGTGTG GGCTGCAACC AGCATTGCCT GTGTCAGAAT
    GGAGGGTCGT GTGACCGAAT CAGTGGCCAC TGCACATGTG TCAGTGGGTG GACAGGAGTG
    GCCTGTGAAT TGGAATGTGC TGCGGGACAC TTTGGCATGG ACTGTCAGCA CAAGTGTGAG
    TGTGTAAATG GCGGCTTGTG TGACCGTCAC AGTGGCAGGT GTTTGTGCCC AGCAGGTTGG
    ACAGGCACGC ACTGTGAGAC AGCGTGCACT CAAGGTTTTT ATGGTCTGGG CTGTAAGGAG
    CGATGCCGGT GTGATCATAA CGCCGCATGC CACCACATCA CTGGCACCTG CTTGTGTCCC
    GCTGGCTGGA GAGGGACTCA CTGCGAAAAG GTTTGTTTAC CTGGTTCTTA TGGGAAGCGT
    TGTGCTCATC GATGCCAGTG TCCTCATGGA ACCTCCTGTA ATCATGTGAC TGGACAGTGT
    GGTTGCCCAG CGGGATTGAC TGGTAGTGGC TGTGAGCGAA TGTGCCAGTC AGGTACGTTT
    GGGGTAAACT GTAATCAGGT GTGCCAGTGC TCTGAGCTCA ACCAGCTCTG CCACCCTGTC
    ACTGGTGTCT GTTACTGTGC TCCCGGATAC TATGGCAGCA AGTGTGAAAC AGAATGTGAA
    GCGGGCCGTT ATGGACCCAA CTGCGAGCAG GAATGCCAAT GTCTGAATAA CGGTGTATGT
    AAAAGCAGCA CTGGCACCTG CAGCTGCCCA CCGGGATTCA TCGGACCAAA CTGCAACATC
    ACGTGTCCAT TAGGGCGTTA TGGTCAGGAC TGTTTTGGAG TGTCTCTGTG TGGTGAAGGG
    GCTCGCAGTG ACCCTCTCAC AGGCAGATGT GTGTGCAGGG CTGGTCAGCG TGGACGGGAC
    TGTGGGAAAG GCTGTGCTGA GGGCTGGTTT GGAGAGAATT GTGATCAGCG CTGTAACTGC
    AGCAATGGAG GAGTGTGTGA CGCTCTTACA GGACACTGCA GCTGTACACT GGGCTGGACA
    GGAGACACCT GTGAGGAAGT GTGTCCCAGA GGACGGTATG GTTCAGACTG CAGTGAAGTG
    TGTGACTGTA AGAATAACAG CACATGCGAC CGAGTGACTG GCGTCTGTCT CTGCTCTCCA
    GGTTTCTACG GACATCTATG TCAACATGCA TGTCCTGTTG GCTTCCATGG TCATCGTTGC
    CAGCTTCTGT GTGACTGTGG TGCAAATGCA CCTTGTGACC CTGAAACTGG CCGATGCCTC
    TGTCCTGCAG GATACCAGGG ACTCCGCTGT GAGAGAGAGT GTGAATTGGG CACATTCGGG
    CCTGATTGTG TCCACAGATG TGACTGTGAT GGAGACACCC CATGTGACCC TATTAATGGC
    AGGTGTTTGT GTGCACCTGG AAAAACAGGC TCTCGCTGTG ACATAGGCTG TGGAGTGAAC
    CGATTTGGAC CCGACTGCTC ACAGAGATGT GAATGTCTGA ACGGAGGTCA GTGTGATCCC
    CGAAACGGCC GATGCACGTG TCTGAACGGC TGGATCGGGC CCAGCTGTCA ACAAGGGCTC
    CTGTCTGCTA GCAAAAGTAA AAGGAAGGAC TCGTCAGTAT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_021329334.1
    CDS109..4650
    Translation

    Target ORF information:

    RefSeq Version XM_021473659.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio multiple EGF-like-domains 6a (megf6a), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_021473659.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    ATGGATTTAC ACGTGTTGTT GTTATTATTA TTCCTGCTAA CCAAGATCCG TCCATCGTGT 
    TGTCTTCGAG CTCTACATCC TTTCATGCCG AATGTTTGCT CAGAGCCAGA GAGAGTCATC
    CGAGATGAGA AACAGCCATG TGTGCAGGCA TTTATACAAA TGGTGAAGGT CTGGAGGCAA
    GGATGCACAG GTCATGCTTG GTGTATGGAC TTCGAGAGGA GGACTACCTA CTACATAGGA
    TATAAACAAG TCTACAGGCA GGACTTCAAG ATGGCATACA AATGCTGTCC TGGATGGTCC
    CAGAACAATT CAAAGATGGG CTGCCTTTAT CATATTGATG AGTGCCAGGT TCACAACGGA
    GGCTGCCAGC ACAGATGTGT GAACACTAGA GGCTCGTACT ACTGCGAGTG CAATCCAGGC
    TTTCGGCTCC ACGTTGATGG CCGCACCTGC ATAGCTGTTT TATCCTGTTC GGTAAATAAT
    GGAGGTTGTG AGCATGAATG TGTCCAGCTT TCTCCAGTTC ACTTCCAGTG CCGCTGCAGA
    CGTGGATACC AGCTTACAGC AGATGGCAAG CGCTGTAGAT TGAGGAACCC ATGTGTGGAG
    AAGAATGGCG GCTGTGTGCA TTTATGTCGC AGTGATGGCG GTCGGGTTGT GTGCAGCTGT
    CACCAAGGCT TCATGCTAGC AATGGACCAG AGGAGCTGTG AAGATGTCAA TGAGTGTGAA
    ACAGGACTGG CAGCATGTGC ACACAGTTGC AGGAACACTC CAGGATCGTT TTCCTGCATC
    TGCAATCCAG GATACGAGCT GGGCTCTGAT GGAAGGAAAT GCTACCGTAT TGAAATGGAG
    ATTGTCAATA CCTGTGACTC TGATAATGGT GGCTGTTCAC ATCGCTGTCA GCATGGTGTT
    CTTGGGCCTG TCTGCAGCTG TAATCATGGT TATCAGCTCG CCGAAGACCT CAAAACCTGT
    TTGGATCTGG AGGAATGTGA GGAAGGAACG TCATGCTGTG AGCAGGATTG CACAAACTAC
    CCTGGAGGGT ATGAATGCTA CTGCAGGGCA GGTTACAGAC TCAATGCAGA TGGATGCGGA
    TGTGACGATG TGGACGAGTG TGCATCTGAT AGAGGAGGCT GTGAACACAC TTGTCAGAAT
    ACGGCTGGCT CATATCACTG CTTCTGTCGG TTTGGATATC GACTTGGTGA GGACAGACAC
    TCCTGCATTT CTCTCCAGCA ATCGGTTGAG GAATTGCCCG GTCCAGTGGT GGTTGAGCGT
    CCTCTCCTTC ATCTCGCTCT CCTCCAGGAT TACCCACAAC CCCTGGAGCG CTATCATGAC
    TATGAGGAGG ACAGTTTTGG AGAGCTGCGG GCAGAGAGCA CCTTCGCTGA GAAGTTTGTG
    TGTTTGAATG GGACGTTTGG CCATGACTGC AGCCTGACAT GTGATGATTG CTCAAATGGA
    GGATATTGTA ATGCTGAAGG AGATGCTTGT GACTGTCCAG ATGGGTGGAC GGGGATCCTC
    TGTAACCAAA CCTGTCCAGA GGGAACATTT GGTAAAAACT GCTCCTTCAC TTGCAAGTGC
    AGAAACGGGG GCACCTGTGA CCCGATTAGT GGGAAGTGCC GCTGCCCACC TGGAGTCAAT
    GGAGACCTGT GCCAGGATGG CTGTCCGAAG GGAATGTATG GAAAGCACTG CAACAAGAAA
    TGTAACTGTG CTAATAACGG CCGCTGCCAT CGCACATATG GAGCCTGTCT GTGTGATCCA
    GGACTCTACG GCAGGTTCTG TCACCTCCCT TGCCCCAAGT GGTCGTATGG ATCTGGCTGC
    TCATCCGAGT GTCAGTGTGT GCAACAAAAC ACGCTTGAGT GCCATCGACG TCATGGAACG
    TGTGTGTGCA AACCTGGCTA TCAGGGGAAA ACCTGCAACC GCGAGTGTGA TAGTGGATTT
    TATGGTCCAG GATGTAGATT AAAATGCCAG TGTCCAGCGG GAGTCTCTTG CCATCCCATG
    AGTGGGCAAT GTCAGCGTCA GTGCCCAGCA GGCCTACATG GGGAGCACTG TGACCGAGAG
    TGCCCCATGG GACAGTTTGG AGTTGGATGC ACACAGTTTT GTGACTGTAA GGGAGCTCCA
    TGTAACTCAG TGACCGGGAA ATGCCACTGT CCTGCAGGGC AGACAGGACA ACACTGTGAA
    AAAGAGTGTG AAGAGGGCCG TTGGGGTCTT GGCTGTGCAG AATCATGTCC TTCCTGTGAC
    AATGGTGGAG TATGTGATAG ACAAAACGGC ACCTGTGTTT GTTCGCCGGG ATTCATGGGA
    AATCTCTGTC AGAATGTCTG TCCAGATGGG CACTTTGGCC TGAGCTGCCT GTTGCGCTGC
    TCCTGTCAAA ACGAAGGGAA ATGCCACCAT GTGACTGGCC TCTGTGTTTG TAAAGACGGC
    TGGATGGGAC CTAACTGCGC AAAAGCTTGT GTGGCTGGAC GATGGGGGCC GAACTGTGCC
    AACCAGTGTG ACTGCAGGAG CAGCGTGGGC AGCTGTGACC CTGTGATTGG CCAGTGTCAT
    TGTGAAGCGG GCTACATGGG ACCACGCTGT GATCAAAAGT GTCCTAAGGG TTTCTTCGGC
    CAGAGCTGCC AGCACAGATG CCAGTGTGAG AATGGCGCAG CGTGTGAGCA TGTGAGCGGT
    GCCTGCACTT GTTTGGCTGG CTGGACTGGG ACGTATTGTG AAAAACCATG TCCTGAGGGC
    TTTCATGGCT TGGAGTGCCA GCAGAAGTGC CAGTGCTTGA ATGGTGGCCG TTGTCATCCC
    GTGACTGGAG GCTGCCAGTG TCCATCTGGT TGGGTCGGGC CAGTTTGCAA TGCCACGTGT
    GAAGCTGGCA CCTTTGGGCC GGCCTGCAGT CAAACCTGTA GATGCCACAA TAATGGCACC
    TGTGACCCTG CTGATGGACG ATGTGAGTGT GGAGCAGGAT GGACAGGCAG CAGGTGTGAA
    CAAGAGTGCT CAGCTGGATT GTATGGTGTG GGCTGCAACC AGCATTGCCT GTGTCAGAAT
    GGAGGGTCGT GTGACCGAAT CAGTGGCCAC TGCACATGTG TCAGTGGGTG GACAGGAGTG
    GCCTGTGAAT TGGAATGTGC TGCGGGACAC TTTGGCATGG ACTGTCAGCA CAAGTGTGAG
    TGTGTAAATG GCGGCTTGTG TGACCGTCAC AGTGGCAGGT GTTTGTGCCC AGCAGGTTGG
    ACAGGCACGC ACTGTGAGAC AGCGTGCACT CAAGGTTTTT ATGGTCTGGG CTGTAAGGAG
    CGATGCCGGT GTGATCATAA CGCCGCATGC CACCACATCA CTGGCACCTG CTTGTGTCCC
    GCTGGCTGGA GAGGGACTCA CTGCGAAAAG GTTTGTTTAC CTGGTTCTTA TGGGAAGCGT
    TGTGCTCATC GATGCCAGTG TCCTCATGGA ACCTCCTGTA ATCATGTGAC TGGACAGTGT
    GGTTGCCCAG CGGGATTGAC TGGTAGTGGC TGTGAGCGAA TGTGCCAGTC AGGTACGTTT
    GGGGTAAACT GTAATCAGGT GTGCCAGTGC TCTGAGCTCA ACCAGCTCTG CCACCCTGTC
    ACTGGTGTCT GTTACTGTGC TCCCGGATAC TATGGCAGCA AGTGTGAAAC AGAATGTGAA
    GCGGGCCGTT ATGGACCCAA CTGCGAGCAG GAATGCCAAT GTCTGAATAA CGGTGTATGT
    AAAAGCAGCA CTGGCACCTG CAGCTGCCCA CCGGGATTCA TCGGACCAAA CTGCAACATC
    ACGTGTCCAT TAGGGCGTTA TGGTCAGGAC TGTTTTGGAG TGTCTCTGTG TGGTGAAGGG
    GCTCGCAGTG ACCCTCTCAC AGGCAGATGT GTGTGCAGGG CTGGTCAGCG TGGACGGGAC
    TGTGGGAAAG GCTGTGCTGA GGGCTGGTTT GGAGAGAATT GTGATCAGCG CTGTAACTGC
    AGCAATGGAG GAGTGTGTGA CGCTCTTACA GGACACTGCA GCTGTACACT GGGCTGGACA
    GGAGACACCT GTGAGGAAGT GTGTCCCAGA GGACGGTATG GTTCAGACTG CAGTGAAGTG
    TGTGACTGTA AGAATAACAG CACATGCGAC CGAGTGACTG GCGTCTGTCT CTGCTCTCCA
    GGTTTCTACG GACATCTATG TCAACATGCA TGTCCTGTTG GCTTCCATGG TCATCGTTGC
    CAGCTTCTGT GTGACTGTGG TGCAAATGCA CCTTGTGACC CTGAAACTGG CCGATGCCTC
    TGTCCTGCAG GATACCAGGG ACTCCGCTGT GAGAGAGAGT GTGAATTGGG CACATTCGGG
    CCTGATTGTG TCCACAGATG TGACTGTGAT GGAGACACCC CATGTGACCC TATTAATGGC
    AGGTGTTTGT GTGCACCTGG AAAAACAGGC TCTCGCTGTG ACATAGGCTG TGGAGTGAAC
    CGATTTGGAC CCGACTGCTC ACAGAGATGT GAATGTCTGA ACGGAGGTCA GTGTGATCCC
    CGAAACGGCC GATGCACGTG TCTGAACGGC TGGATCGGGC CCAGCTGTCA ACAAGGGCTC
    CTGTCTGCTA GCAAAAGTAA AAGGAAGGAC TCGTCAGTAT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa151555
    Clone ID Related Accession (Same CDS sequence) XM_017353739.2
    Accession Version XM_017353739.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4542bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product multiple epidermal growth factor-like domains protein 6 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007134.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_017353739.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    ATGGATTTAC ACGTGTTGTT GTTATTATTA TTCCTGCTAA CCAAGATCCG TCCATCGTGT 
    TGTCTTCGAG CTCTACATCC TTTCATGCCG AATGTTTGCT CAGAGCCAGA GAGAGTCATC
    CGAGCTGAGA AACAGCCATG TGTGCAGGCA TTTATACAAA TGGTGAAGGT CTGGAGGCAA
    GGATGCACAG GTCATGCTTG GTGTATGGAC TTCGAGAGGA GGACTACCTA CTACATAGGA
    TATAAACAAG TCTACAGGCA GGACTTCAAG ATGGCATACA AATGCTGTCC TGGATGGTCC
    CAGAACAATT CAAAGATGGG CTGCCTTTAT CATATTGATG AGTGCCAGGT TCACAACGGA
    GGCTGCCAGC ACAGATGTGT GAACACTAGA GGCTCGTACT ACTGCGAGTG CAATCCAGGC
    TTTCGGCTCC ACGTTGATGG CCGCACCTGC ATAGCTGTTT TATCCTGTTC GGTAAATAAT
    GGAGGTTGTG AGCATGAATG TGTCCAGCTT TCTCCAGTTC ACTTCCAGTG CCGCTGCAGA
    CGTGGATACC AGCTTACAGC AGATGGCAAG CGCTGTAGAT TGAGGAACCC ATGTGTGGAG
    AAGAATGGCG GCTGTGTGCA TTTATGTCGC AGTGATGGTG GTCGGGTTGT GTGCAGCTGT
    CACCAAGGCT TCATGCTAGC AGTGGACCAG AAGAGCTGTG AAGATGTCAA TGAGTGTGAA
    ACAGGACTGG CAGCATGTGC ACACAGTTGC AGGAACACTC CAGGATCGTT TTCCTGCATC
    TGCAATCCAG GATACGAGCT GGGCTCTGAT GGAAGGAAAT GCTACCGTAT TGAAATGGAG
    ATTGTCAATA CCTGTGACTC TGATAATGGT GGCTGTTCAC ATCGCTGTCA GCATGGTGTT
    CTTGGGCCTG TCTGCAGCTG TAATCATGGT TATCAGCTCG CCGAAGACCT CAAAACCTGT
    TTGGATCTGG AGGAATGTGA GGAAGGAACG TCATGCTGTG AGCAGGATTG CACAAACTAC
    CCTGGAGGGT ATGAATGCTA CTGCAGGGCA GGTTACAGAC TCAATGCAGA TGGATGCGGA
    TGTGACGATG TGGACGAGTG TGCGTCTGAT AGAGGAGGCT GTGAACACAC TTGTCAGAAT
    ACGGCTGGCT CATATCACTG CTTCTGTCGG TTTGGATATC GACTTGGTGA GGACAGACAC
    TCCTGCATTT CTCTCCAGCG ATCGGTTGAG GAATTGCCCG GTCCAGTGGT GGTTGAGCGT
    CCTCTCCTTC ATCTCGCTCT CCTCCAGGAT TACCCACAAC CCCTGGAGCG CTATCATGAC
    TATGAGGAGG ACAGTTTTGG AGAGCTGCGG GCAGAGAGCA CCTTCGCTGA GAAGTTTGTG
    TGTTTGAATG GGACGTTTGG CCATGACTGC AGCCTGACGT GTGATGATTG CTCAAATGGA
    GGATATTGTA ATGCTGAAGG AGATGCTTGT GACTGTCCAG ATGGGTGGAC GGGGATCCTT
    TGTAACCAAA CCTGTCCAGA GGGAACATTT GGTAAAAACT GCTCCTTCAC TTGCAAGTGC
    AGAAACGGGG GCACCTGTGA CCCGATTAGT GGGAAGTGCC GCTGCCCACC TGGAGTCAAT
    GGAGACCTGT GCCAGGACGG CTGTCCGAAG GGAATGTATG GAAAGCACTG CAACAAGAAA
    TGTAACTGTG CTAATAACGG CCGCTGCCAT CGCACATATG GAGCCTGTCT GTGTGATCCA
    GGACTCTACG GCAGGTTCTG TCACCTCCCT TGCCCCAAGT GGTCGTATGG ATCTGGCTGC
    TCATCCGAGT GTCAGTGTGT GCAACAAAAC ACGCTTGAGT GCCATCGACG TCATGGAACG
    TGTGTGTGCA AACCTGGCTA TCAGGGGAAA ACCTGCAACC GCGAGTGTGA TAGTGGATTT
    TATGGTCCAG GATGTAGATT AAAATGCCAG TGTCCAGCGG GAGTCTCTTG CCATCCCATG
    AGTGGGCAAT GTCAGCGTCA GTGCCCAGCA GGCCTACATG GGGAGCACTG TGACCGAGAG
    TGCCCCATGG GACAGTTTGG AGTTGGATGC ACACAGTTTT GTGACTGTAA GGGAGCTCCA
    TGTAACTCAG TGACCGGGAA ATGCCACTGT CCTGCAGGGC AGACAGGACA ACACTGTGAA
    AAAGAGTGTG AAGAGGGCCG TTGGGGTCTT GGCTGTGCAG AATCATGTCC TTCCTGTGAC
    AATGGTGGAG TATGTGATAG ACAAAACGGC ACCTGTGTTT GTTCGCCGGG ATTCATGGGA
    AATCTCTGTC AGAATGTCTG TCCAGATGGG CACTTTGGCC TGAGCTGCCT GTTGCGCTGC
    TCCTGTCAAA ACGAAGGGAA ATGCCACCAT GTGACTGGCC TCTGTGTTTG TAAAGATGGC
    TGGATGGGAC CCAACTGCGC AAAAGCTTGT GTGGCTGGAC GATGGGGGCC GAACTGTGCC
    AACCAGTGTG ACTGCAGGAG CAGCGTGGGC AGCTGTGACC CTGTGATTGG CCTGTGTCAT
    TGTGAAGCGG GCTACATGGG ACCACGCTGT GATCAAAAGT GTCCTCAGGG TTTCTTCGGC
    CAGAGCTGCC AGCACAGATG CCAGTGTGAG AATGGCGCAG TGTGTGAGCA TGTGAGCGGT
    GCCTGCACTT GTTTGGCTGG CTGGACTGGG ACGTATTGTG AAAAACCTTG TCCTGAGGGC
    TTTCATGGCT TGGAGTGCCA GCAGAAGTGC CAGTGCTTGA ATGGTGGCCG TTGTCATCCC
    GTGACTGGAG GCTGCCAGTG TCCATCTGGT TGGGTCGGGC CAGTTTGCAA TGCCACGTGT
    GAAGCTGGCA CCTTTGGGCC GGCCTGCAGT CAAACCTGTA GATGCCACAA TAATGGCACC
    TGTGACCCTG CTGATGGGCG ATGTGAGTGT GGAGCAGGAT GGACAGGCAG CAGCTGTGAA
    CAAGAGTGCT CAGCTGGATT GTATGGTGTG GGCTGCAACC AGCATTGCCT GTGTCAGAAT
    GGAGGGTCGT GTGACCGAAT CAGTGGCCAC TGCACATGTG TCAGTGGGTG GACAGGAGTG
    GCCTGTGAAT TGGAATGTGC TGCGGGACAC TTTGGCATGG ACTGTCAGCA CAAGTGTGAG
    TGTGTAAATG GCGGCGTGTG TGACCGTCAC AGTGGCAGGT GTTTGTGCCC AGCAGGTTGG
    ACAGGCACGC ACTGTGAGAC AGCATGCACT CAAGGTTTTT ATGGTCCGGG CTGTAAGGAG
    CGATGCCGGT GTGATCATAA CGCCGCATGC CACCACATCA CTGGCACCTG CTTGTGTCCC
    GCTGGCTGGA GAGGGACTCA CTGCGAAAAG GTTTGTTTAC CTGGTTCCTA TGGGAAGCGT
    TGTGCTCATC GATGCCAGTG TCCTCATGGA ACCTCCTGTA ACCATGTGAC TGGACAGTGT
    GGTTGCCCAG CGGGATTAAC TGGTAGTGGC TGTGAGCGAA TGTGCCAGTC AGGTACGTTT
    GGGGTAAACT GTAATCAGGT GTGCCAGTGC TCTGAGCTCA ACCAGCTCTG CCACCCCGTC
    ACTGGTGTCT GTTACTGTGC TCCCGGATAC TATGGCAGCA AGTGTGAAAC AGAATGTGAA
    GCGGGCCGTT ATGGACCCAA CTGTGAGCAG GAATGCCAGT GTCTGAATAA CGGTGTATGT
    AAAAGCAGCA CTGGCACCTG CAGCTGCCCA CCGGGATTCA TCGGACCAAA CTGCAACATC
    ACATGTCCAT TAGGGCGTTA TGGTCAGGAC TGTGCTGGAG TGTCTCTGTG TGGTGAAGGG
    GCTCGCAGTG ACCCTCTCAC AGGCAGATGT GTGTGCAGGG CTGGTCAGCG TGGACGGGAC
    TGTGGGAAAG GCTGTGCTGA GGGCTGGTTT GGAGAGAATT GTGATCAGCG CTGTAACTGC
    AGCAATGGCG GAGTGTGTGA CGCTCTTACA GGACACTGCA GCTGTACACT GGGCTGGACA
    GGAGACACCT GTGAGGAAGT GTGTCCCAGA GGACGGTATG GTTCAGACTG CAGTGAAGTG
    TGTGACTGTA AGAATAACAG CACATGCGAC CGAGTGACTG GCGTCTGTCT CTGCTCTCCA
    GGTTTCTACG GACATCTATG TCAACATGCA TGTCCTGTTG GCTTCCATGG TCATCGTTGC
    CAGCTTCTGT GTGACTGTGG TGCAAATGCA CCTTGTGACC CTGAAACTGG CCGATGCCTC
    TGTCCTGCAG GATACCAGGG ACTCCGCTGT GAGAGAGAGT GTGAATTGGG CACATTTGGG
    GCTGATTGTG TCCACAGATG TGACTGTGAT GGAGACACCC CATGTGACCC TATTAATGGC
    AGGTGTTTGT GTGCACCTGG AAAAACAGGC TCTCGCTGTG ACATAGGCTG TGGAGTGAAC
    CGATTTGGAC CCGACTGCTC ACAGAGATGT GAATGTCTGA ACGGAGGTCA GTGTGATCCC
    CGAAACGGCC GATGCACGTG TCTGAACGGC TGGATCGGGC CCAGCTGTCA ACAAGGGCTC
    CTGTCTGCTA GCAAAAGTAA AAGGAAGGAC TCGTCAGTAT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017209228.2
    CDS111..4652
    Translation

    Target ORF information:

    RefSeq Version XM_017353739.2
    Organism Danio rerio(zebrafish)
    Definition Danio rerio multiple EGF-like-domains 6a (megf6a), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017353739.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    ATGGATTTAC ACGTGTTGTT GTTATTATTA TTCCTGCTAA CCAAGATCCG TCCATCGTGT 
    TGTCTTCGAG CTCTACATCC TTTCATGCCG AATGTTTGCT CAGAGCCAGA GAGAGTCATC
    CGAGCTGAGA AACAGCCATG TGTGCAGGCA TTTATACAAA TGGTGAAGGT CTGGAGGCAA
    GGATGCACAG GTCATGCTTG GTGTATGGAC TTCGAGAGGA GGACTACCTA CTACATAGGA
    TATAAACAAG TCTACAGGCA GGACTTCAAG ATGGCATACA AATGCTGTCC TGGATGGTCC
    CAGAACAATT CAAAGATGGG CTGCCTTTAT CATATTGATG AGTGCCAGGT TCACAACGGA
    GGCTGCCAGC ACAGATGTGT GAACACTAGA GGCTCGTACT ACTGCGAGTG CAATCCAGGC
    TTTCGGCTCC ACGTTGATGG CCGCACCTGC ATAGCTGTTT TATCCTGTTC GGTAAATAAT
    GGAGGTTGTG AGCATGAATG TGTCCAGCTT TCTCCAGTTC ACTTCCAGTG CCGCTGCAGA
    CGTGGATACC AGCTTACAGC AGATGGCAAG CGCTGTAGAT TGAGGAACCC ATGTGTGGAG
    AAGAATGGCG GCTGTGTGCA TTTATGTCGC AGTGATGGTG GTCGGGTTGT GTGCAGCTGT
    CACCAAGGCT TCATGCTAGC AGTGGACCAG AAGAGCTGTG AAGATGTCAA TGAGTGTGAA
    ACAGGACTGG CAGCATGTGC ACACAGTTGC AGGAACACTC CAGGATCGTT TTCCTGCATC
    TGCAATCCAG GATACGAGCT GGGCTCTGAT GGAAGGAAAT GCTACCGTAT TGAAATGGAG
    ATTGTCAATA CCTGTGACTC TGATAATGGT GGCTGTTCAC ATCGCTGTCA GCATGGTGTT
    CTTGGGCCTG TCTGCAGCTG TAATCATGGT TATCAGCTCG CCGAAGACCT CAAAACCTGT
    TTGGATCTGG AGGAATGTGA GGAAGGAACG TCATGCTGTG AGCAGGATTG CACAAACTAC
    CCTGGAGGGT ATGAATGCTA CTGCAGGGCA GGTTACAGAC TCAATGCAGA TGGATGCGGA
    TGTGACGATG TGGACGAGTG TGCGTCTGAT AGAGGAGGCT GTGAACACAC TTGTCAGAAT
    ACGGCTGGCT CATATCACTG CTTCTGTCGG TTTGGATATC GACTTGGTGA GGACAGACAC
    TCCTGCATTT CTCTCCAGCG ATCGGTTGAG GAATTGCCCG GTCCAGTGGT GGTTGAGCGT
    CCTCTCCTTC ATCTCGCTCT CCTCCAGGAT TACCCACAAC CCCTGGAGCG CTATCATGAC
    TATGAGGAGG ACAGTTTTGG AGAGCTGCGG GCAGAGAGCA CCTTCGCTGA GAAGTTTGTG
    TGTTTGAATG GGACGTTTGG CCATGACTGC AGCCTGACGT GTGATGATTG CTCAAATGGA
    GGATATTGTA ATGCTGAAGG AGATGCTTGT GACTGTCCAG ATGGGTGGAC GGGGATCCTT
    TGTAACCAAA CCTGTCCAGA GGGAACATTT GGTAAAAACT GCTCCTTCAC TTGCAAGTGC
    AGAAACGGGG GCACCTGTGA CCCGATTAGT GGGAAGTGCC GCTGCCCACC TGGAGTCAAT
    GGAGACCTGT GCCAGGACGG CTGTCCGAAG GGAATGTATG GAAAGCACTG CAACAAGAAA
    TGTAACTGTG CTAATAACGG CCGCTGCCAT CGCACATATG GAGCCTGTCT GTGTGATCCA
    GGACTCTACG GCAGGTTCTG TCACCTCCCT TGCCCCAAGT GGTCGTATGG ATCTGGCTGC
    TCATCCGAGT GTCAGTGTGT GCAACAAAAC ACGCTTGAGT GCCATCGACG TCATGGAACG
    TGTGTGTGCA AACCTGGCTA TCAGGGGAAA ACCTGCAACC GCGAGTGTGA TAGTGGATTT
    TATGGTCCAG GATGTAGATT AAAATGCCAG TGTCCAGCGG GAGTCTCTTG CCATCCCATG
    AGTGGGCAAT GTCAGCGTCA GTGCCCAGCA GGCCTACATG GGGAGCACTG TGACCGAGAG
    TGCCCCATGG GACAGTTTGG AGTTGGATGC ACACAGTTTT GTGACTGTAA GGGAGCTCCA
    TGTAACTCAG TGACCGGGAA ATGCCACTGT CCTGCAGGGC AGACAGGACA ACACTGTGAA
    AAAGAGTGTG AAGAGGGCCG TTGGGGTCTT GGCTGTGCAG AATCATGTCC TTCCTGTGAC
    AATGGTGGAG TATGTGATAG ACAAAACGGC ACCTGTGTTT GTTCGCCGGG ATTCATGGGA
    AATCTCTGTC AGAATGTCTG TCCAGATGGG CACTTTGGCC TGAGCTGCCT GTTGCGCTGC
    TCCTGTCAAA ACGAAGGGAA ATGCCACCAT GTGACTGGCC TCTGTGTTTG TAAAGATGGC
    TGGATGGGAC CCAACTGCGC AAAAGCTTGT GTGGCTGGAC GATGGGGGCC GAACTGTGCC
    AACCAGTGTG ACTGCAGGAG CAGCGTGGGC AGCTGTGACC CTGTGATTGG CCTGTGTCAT
    TGTGAAGCGG GCTACATGGG ACCACGCTGT GATCAAAAGT GTCCTCAGGG TTTCTTCGGC
    CAGAGCTGCC AGCACAGATG CCAGTGTGAG AATGGCGCAG TGTGTGAGCA TGTGAGCGGT
    GCCTGCACTT GTTTGGCTGG CTGGACTGGG ACGTATTGTG AAAAACCTTG TCCTGAGGGC
    TTTCATGGCT TGGAGTGCCA GCAGAAGTGC CAGTGCTTGA ATGGTGGCCG TTGTCATCCC
    GTGACTGGAG GCTGCCAGTG TCCATCTGGT TGGGTCGGGC CAGTTTGCAA TGCCACGTGT
    GAAGCTGGCA CCTTTGGGCC GGCCTGCAGT CAAACCTGTA GATGCCACAA TAATGGCACC
    TGTGACCCTG CTGATGGGCG ATGTGAGTGT GGAGCAGGAT GGACAGGCAG CAGCTGTGAA
    CAAGAGTGCT CAGCTGGATT GTATGGTGTG GGCTGCAACC AGCATTGCCT GTGTCAGAAT
    GGAGGGTCGT GTGACCGAAT CAGTGGCCAC TGCACATGTG TCAGTGGGTG GACAGGAGTG
    GCCTGTGAAT TGGAATGTGC TGCGGGACAC TTTGGCATGG ACTGTCAGCA CAAGTGTGAG
    TGTGTAAATG GCGGCGTGTG TGACCGTCAC AGTGGCAGGT GTTTGTGCCC AGCAGGTTGG
    ACAGGCACGC ACTGTGAGAC AGCATGCACT CAAGGTTTTT ATGGTCCGGG CTGTAAGGAG
    CGATGCCGGT GTGATCATAA CGCCGCATGC CACCACATCA CTGGCACCTG CTTGTGTCCC
    GCTGGCTGGA GAGGGACTCA CTGCGAAAAG GTTTGTTTAC CTGGTTCCTA TGGGAAGCGT
    TGTGCTCATC GATGCCAGTG TCCTCATGGA ACCTCCTGTA ACCATGTGAC TGGACAGTGT
    GGTTGCCCAG CGGGATTAAC TGGTAGTGGC TGTGAGCGAA TGTGCCAGTC AGGTACGTTT
    GGGGTAAACT GTAATCAGGT GTGCCAGTGC TCTGAGCTCA ACCAGCTCTG CCACCCCGTC
    ACTGGTGTCT GTTACTGTGC TCCCGGATAC TATGGCAGCA AGTGTGAAAC AGAATGTGAA
    GCGGGCCGTT ATGGACCCAA CTGTGAGCAG GAATGCCAGT GTCTGAATAA CGGTGTATGT
    AAAAGCAGCA CTGGCACCTG CAGCTGCCCA CCGGGATTCA TCGGACCAAA CTGCAACATC
    ACATGTCCAT TAGGGCGTTA TGGTCAGGAC TGTGCTGGAG TGTCTCTGTG TGGTGAAGGG
    GCTCGCAGTG ACCCTCTCAC AGGCAGATGT GTGTGCAGGG CTGGTCAGCG TGGACGGGAC
    TGTGGGAAAG GCTGTGCTGA GGGCTGGTTT GGAGAGAATT GTGATCAGCG CTGTAACTGC
    AGCAATGGCG GAGTGTGTGA CGCTCTTACA GGACACTGCA GCTGTACACT GGGCTGGACA
    GGAGACACCT GTGAGGAAGT GTGTCCCAGA GGACGGTATG GTTCAGACTG CAGTGAAGTG
    TGTGACTGTA AGAATAACAG CACATGCGAC CGAGTGACTG GCGTCTGTCT CTGCTCTCCA
    GGTTTCTACG GACATCTATG TCAACATGCA TGTCCTGTTG GCTTCCATGG TCATCGTTGC
    CAGCTTCTGT GTGACTGTGG TGCAAATGCA CCTTGTGACC CTGAAACTGG CCGATGCCTC
    TGTCCTGCAG GATACCAGGG ACTCCGCTGT GAGAGAGAGT GTGAATTGGG CACATTTGGG
    GCTGATTGTG TCCACAGATG TGACTGTGAT GGAGACACCC CATGTGACCC TATTAATGGC
    AGGTGTTTGT GTGCACCTGG AAAAACAGGC TCTCGCTGTG ACATAGGCTG TGGAGTGAAC
    CGATTTGGAC CCGACTGCTC ACAGAGATGT GAATGTCTGA ACGGAGGTCA GTGTGATCCC
    CGAAACGGCC GATGCACGTG TCTGAACGGC TGGATCGGGC CCAGCTGTCA ACAAGGGCTC
    CTGTCTGCTA GCAAAAGTAA AAGGAAGGAC TCGTCAGTAT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa44516
    Clone ID Related Accession (Same CDS sequence) XM_009296882.2 , XM_009296882.3
    Accession Version XM_009296882.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4371bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product multiple epidermal growth factor-like domains protein 6 isoform X2
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007134.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009296882.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    ATGTTATCCG ATTGGTTGAG GTTGGTGCTT GTGTTTACCT TCTGGACTCA GCTGGTACCA 
    AGTTTGGCTG CTGGAAATCC ACATTACCGC TACTATCACA GAAACTGCTA CACCTGCTTC
    AATGGATATC ATGCGTACAA CACAGGACAC CAGGGCGCAG ATATTGATGA GTGCCAGGTT
    CACAACGGAG GCTGCCAGCA CAGATGTGTG AACACTAGAG GCTCGTACTA CTGCGAGTGC
    AATCCAGGCT TTCGGCTCCA CGTTGATGGC CGCACCTGCA TAGCTGTTTT ATCCTGTTCG
    GTAAATAATG GAGGTTGTGA GCATGAATGT GTCCAGCTTT CTCCAGTTCA CTTCCAGTGC
    CGCTGCAGAC GTGGATACCA GCTTACAGCA GATGGCAAGC GCTGTAGATT GAGGAACCCA
    TGTGTGGAGA AGAATGGCGG CTGTGTGCAT TTATGTCGCA GTGATGGTGG TCGGGTTGTG
    TGCAGCTGTC ACCAAGGCTT CATGCTAGCA GTGGACCAGA AGAGCTGTGA AGATGTCAAT
    GAGTGTGAAA CAGGACTGGC AGCATGTGCA CACAGTTGCA GGAACACTCC AGGATCGTTT
    TCCTGCATCT GCAATCCAGG ATACGAGCTG GGCTCTGATG GAAGGAAATG CTACCGTATT
    GAAATGGAGA TTGTCAATAC CTGTGACTCT GATAATGGTG GCTGTTCACA TCGCTGTCAG
    CATGGTGTTC TTGGGCCTGT CTGCAGCTGT AATCATGGTT ATCAGCTCGC CGAAGACCTC
    AAAACCTGTT TGGATCTGGA GGAATGTGAG GAAGGAACGT CATGCTGTGA GCAGGATTGC
    ACAAACTACC CTGGAGGGTA TGAATGCTAC TGCAGGGCAG GTTACAGACT CAATGCAGAT
    GGATGCGGAT GTGACGATGT GGACGAGTGT GCGTCTGATA GAGGAGGCTG TGAACACACT
    TGTCAGAATA CGGCTGGCTC ATATCACTGC TTCTGTCGGT TTGGATATCG ACTTGGTGAG
    GACAGACACT CCTGCATTTC TCTCCAGCGA TCGGTTGAGG AATTGCCCGG TCCAGTGGTG
    GTTGAGCGTC CTCTCCTTCA TCTCGCTCTC CTCCAGGATT ACCCACAACC CCTGGAGCGC
    TATCATGACT ATGAGGAGGA CAGTTTTGGA GAGCTGCGGG CAGAGAGCAC CTTCGCTGAG
    AAGTTTGTGT GTTTGAATGG GACGTTTGGC CATGACTGCA GCCTGACGTG TGATGATTGC
    TCAAATGGAG GATATTGTAA TGCTGAAGGA GATGCTTGTG ACTGTCCAGA TGGGTGGACG
    GGGATCCTTT GTAACCAAAC CTGTCCAGAG GGAACATTTG GTAAAAACTG CTCCTTCACT
    TGCAAGTGCA GAAACGGGGG CACCTGTGAC CCGATTAGTG GGAAGTGCCG CTGCCCACCT
    GGAGTCAATG GAGACCTGTG CCAGGACGGC TGTCCGAAGG GAATGTATGG AAAGCACTGC
    AACAAGAAAT GTAACTGTGC TAATAACGGC CGCTGCCATC GCACATATGG AGCCTGTCTG
    TGTGATCCAG GACTCTACGG CAGGTTCTGT CACCTCCCTT GCCCCAAGTG GTCGTATGGA
    TCTGGCTGCT CATCCGAGTG TCAGTGTGTG CAACAAAACA CGCTTGAGTG CCATCGACGT
    CATGGAACGT GTGTGTGCAA ACCTGGCTAT CAGGGGAAAA CCTGCAACCG CGAGTGTGAT
    AGTGGATTTT ATGGTCCAGG ATGTAGATTA AAATGCCAGT GTCCAGCGGG AGTCTCTTGC
    CATCCCATGA GTGGGCAATG TCAGCGTCAG TGCCCAGCAG GCCTACATGG GGAGCACTGT
    GACCGAGAGT GCCCCATGGG ACAGTTTGGA GTTGGATGCA CACAGTTTTG TGACTGTAAG
    GGAGCTCCAT GTAACTCAGT GACCGGGAAA TGCCACTGTC CTGCAGGGCA GACAGGACAA
    CACTGTGAAA AAGAGTGTGA AGAGGGCCGT TGGGGTCTTG GCTGTGCAGA ATCATGTCCT
    TCCTGTGACA ATGGTGGAGT ATGTGATAGA CAAAACGGCA CCTGTGTTTG TTCGCCGGGA
    TTCATGGGAA ATCTCTGTCA GAATGTCTGT CCAGATGGGC ACTTTGGCCT GAGCTGCCTG
    TTGCGCTGCT CCTGTCAAAA CGAAGGGAAA TGCCACCATG TGACTGGCCT CTGTGTTTGT
    AAAGATGGCT GGATGGGACC CAACTGCGCA AAAGCTTGTG TGGCTGGACG ATGGGGGCCG
    AACTGTGCCA ACCAGTGTGA CTGCAGGAGC AGCGTGGGCA GCTGTGACCC TGTGATTGGC
    CTGTGTCATT GTGAAGCGGG CTACATGGGA CCACGCTGTG ATCAAAAGTG TCCTCAGGGT
    TTCTTCGGCC AGAGCTGCCA GCACAGATGC CAGTGTGAGA ATGGCGCAGT GTGTGAGCAT
    GTGAGCGGTG CCTGCACTTG TTTGGCTGGC TGGACTGGGA CGTATTGTGA AAAACCTTGT
    CCTGAGGGCT TTCATGGCTT GGAGTGCCAG CAGAAGTGCC AGTGCTTGAA TGGTGGCCGT
    TGTCATCCCG TGACTGGAGG CTGCCAGTGT CCATCTGGTT GGGTCGGGCC AGTTTGCAAT
    GCCACGTGTG AAGCTGGCAC CTTTGGGCCG GCCTGCAGTC AAACCTGTAG ATGCCACAAT
    AATGGCACCT GTGACCCTGC TGATGGGCGA TGTGAGTGTG GAGCAGGATG GACAGGCAGC
    AGCTGTGAAC AAGAGTGCTC AGCTGGATTG TATGGTGTGG GCTGCAACCA GCATTGCCTG
    TGTCAGAATG GAGGGTCGTG TGACCGAATC AGTGGCCACT GCACATGTGT CAGTGGGTGG
    ACAGGAGTGG CCTGTGAATT GGAATGTGCT GCGGGACACT TTGGCATGGA CTGTCAGCAC
    AAGTGTGAGT GTGTAAATGG CGGCGTGTGT GACCGTCACA GTGGCAGGTG TTTGTGCCCA
    GCAGGTTGGA CAGGCACGCA CTGTGAGACA GCATGCACTC AAGGTTTTTA TGGTCCGGGC
    TGTAAGGAGC GATGCCGGTG TGATCATAAC GCCGCATGCC ACCACATCAC TGGCACCTGC
    TTGTGTCCCG CTGGCTGGAG AGGGACTCAC TGCGAAAAGG TTTGTTTACC TGGTTCCTAT
    GGGAAGCGTT GTGCTCATCG ATGCCAGTGT CCTCATGGAA CCTCCTGTAA CCATGTGACT
    GGACAGTGTG GTTGCCCAGC GGGATTAACT GGTAGTGGCT GTGAGCGAAT GTGCCAGTCA
    GGTACGTTTG GGGTAAACTG TAATCAGGTG TGCCAGTGCT CTGAGCTCAA CCAGCTCTGC
    CACCCCGTCA CTGGTGTCTG TTACTGTGCT CCCGGATACT ATGGCAGCAA GTGTGAAACA
    GAATGTGAAG CGGGCCGTTA TGGACCCAAC TGTGAGCAGG AATGCCAGTG TCTGAATAAC
    GGTGTATGTA AAAGCAGCAC TGGCACCTGC AGCTGCCCAC CGGGATTCAT CGGACCAAAC
    TGCAACATCA CATGTCCATT AGGGCGTTAT GGTCAGGACT GTGCTGGAGT GTCTCTGTGT
    GGTGAAGGGG CTCGCAGTGA CCCTCTCACA GGCAGATGTG TGTGCAGGGC TGGTCAGCGT
    GGACGGGACT GTGGGAAAGG CTGTGCTGAG GGCTGGTTTG GAGAGAATTG TGATCAGCGC
    TGTAACTGCA GCAATGGCGG AGTGTGTGAC GCTCTTACAG GACACTGCAG CTGTACACTG
    GGCTGGACAG GAGACACCTG TGAGGAAGTG TGTCCCAGAG GACGGTATGG TTCAGACTGC
    AGTGAAGTGT GTGACTGTAA GAATAACAGC ACATGCGACC GAGTGACTGG CGTCTGTCTC
    TGCTCTCCAG GTTTCTACGG ACATCTATGT CAACATGCAT GTCCTGTTGG CTTCCATGGT
    CATCGTTGCC AGCTTCTGTG TGACTGTGGT GCAAATGCAC CTTGTGACCC TGAAACTGGC
    CGATGCCTCT GTCCTGCAGG ATACCAGGGA CTCCGCTGTG AGAGAGAGTG TGAATTGGGC
    ACATTTGGGG CTGATTGTGT CCACAGATGT GACTGTGATG GAGACACCCC ATGTGACCCT
    ATTAATGGCA GGTGTTTGTG TGCACCTGGA AAAACAGGCT CTCGCTGTGA CATAGGCTGT
    GGAGTGAACC GATTTGGACC CGACTGCTCA CAGAGATGTG AATGTCTGAA CGGAGGTCAG
    TGTGATCCCC GAAACGGCCG ATGCACGTGT CTGAACGGCT GGATCGGGCC CAGCTGTCAA
    CAAGGGCTCC TGTCTGCTAG CAAAAGTAAA AGGAAGGACT CGTCAGTATA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_009295157.1
    CDS237..4607
    Translation

    Target ORF information:

    RefSeq Version XM_009296882.2
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio multiple EGF-like-domains 6a (megf6a), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_009296882.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    ATGTTATCCG ATTGGTTGAG GTTGGTGCTT GTGTTTACCT TCTGGACTCA GCTGGTACCA 
    AGTTTGGCTG CTGGAAATCC ACATTACCGC TACTATCACA GAAACTGCTA CACCTGCTTC
    AATGGATATC ATGCGTACAA CACAGGACAC CAGGGCGCAG ATATTGATGA GTGCCAGGTT
    CACAACGGAG GCTGCCAGCA CAGATGTGTG AACACTAGAG GCTCGTACTA CTGCGAGTGC
    AATCCAGGCT TTCGGCTCCA CGTTGATGGC CGCACCTGCA TAGCTGTTTT ATCCTGTTCG
    GTAAATAATG GAGGTTGTGA GCATGAATGT GTCCAGCTTT CTCCAGTTCA CTTCCAGTGC
    CGCTGCAGAC GTGGATACCA GCTTACAGCA GATGGCAAGC GCTGTAGATT GAGGAACCCA
    TGTGTGGAGA AGAATGGCGG CTGTGTGCAT TTATGTCGCA GTGATGGTGG TCGGGTTGTG
    TGCAGCTGTC ACCAAGGCTT CATGCTAGCA GTGGACCAGA AGAGCTGTGA AGATGTCAAT
    GAGTGTGAAA CAGGACTGGC AGCATGTGCA CACAGTTGCA GGAACACTCC AGGATCGTTT
    TCCTGCATCT GCAATCCAGG ATACGAGCTG GGCTCTGATG GAAGGAAATG CTACCGTATT
    GAAATGGAGA TTGTCAATAC CTGTGACTCT GATAATGGTG GCTGTTCACA TCGCTGTCAG
    CATGGTGTTC TTGGGCCTGT CTGCAGCTGT AATCATGGTT ATCAGCTCGC CGAAGACCTC
    AAAACCTGTT TGGATCTGGA GGAATGTGAG GAAGGAACGT CATGCTGTGA GCAGGATTGC
    ACAAACTACC CTGGAGGGTA TGAATGCTAC TGCAGGGCAG GTTACAGACT CAATGCAGAT
    GGATGCGGAT GTGACGATGT GGACGAGTGT GCGTCTGATA GAGGAGGCTG TGAACACACT
    TGTCAGAATA CGGCTGGCTC ATATCACTGC TTCTGTCGGT TTGGATATCG ACTTGGTGAG
    GACAGACACT CCTGCATTTC TCTCCAGCGA TCGGTTGAGG AATTGCCCGG TCCAGTGGTG
    GTTGAGCGTC CTCTCCTTCA TCTCGCTCTC CTCCAGGATT ACCCACAACC CCTGGAGCGC
    TATCATGACT ATGAGGAGGA CAGTTTTGGA GAGCTGCGGG CAGAGAGCAC CTTCGCTGAG
    AAGTTTGTGT GTTTGAATGG GACGTTTGGC CATGACTGCA GCCTGACGTG TGATGATTGC
    TCAAATGGAG GATATTGTAA TGCTGAAGGA GATGCTTGTG ACTGTCCAGA TGGGTGGACG
    GGGATCCTTT GTAACCAAAC CTGTCCAGAG GGAACATTTG GTAAAAACTG CTCCTTCACT
    TGCAAGTGCA GAAACGGGGG CACCTGTGAC CCGATTAGTG GGAAGTGCCG CTGCCCACCT
    GGAGTCAATG GAGACCTGTG CCAGGACGGC TGTCCGAAGG GAATGTATGG AAAGCACTGC
    AACAAGAAAT GTAACTGTGC TAATAACGGC CGCTGCCATC GCACATATGG AGCCTGTCTG
    TGTGATCCAG GACTCTACGG CAGGTTCTGT CACCTCCCTT GCCCCAAGTG GTCGTATGGA
    TCTGGCTGCT CATCCGAGTG TCAGTGTGTG CAACAAAACA CGCTTGAGTG CCATCGACGT
    CATGGAACGT GTGTGTGCAA ACCTGGCTAT CAGGGGAAAA CCTGCAACCG CGAGTGTGAT
    AGTGGATTTT ATGGTCCAGG ATGTAGATTA AAATGCCAGT GTCCAGCGGG AGTCTCTTGC
    CATCCCATGA GTGGGCAATG TCAGCGTCAG TGCCCAGCAG GCCTACATGG GGAGCACTGT
    GACCGAGAGT GCCCCATGGG ACAGTTTGGA GTTGGATGCA CACAGTTTTG TGACTGTAAG
    GGAGCTCCAT GTAACTCAGT GACCGGGAAA TGCCACTGTC CTGCAGGGCA GACAGGACAA
    CACTGTGAAA AAGAGTGTGA AGAGGGCCGT TGGGGTCTTG GCTGTGCAGA ATCATGTCCT
    TCCTGTGACA ATGGTGGAGT ATGTGATAGA CAAAACGGCA CCTGTGTTTG TTCGCCGGGA
    TTCATGGGAA ATCTCTGTCA GAATGTCTGT CCAGATGGGC ACTTTGGCCT GAGCTGCCTG
    TTGCGCTGCT CCTGTCAAAA CGAAGGGAAA TGCCACCATG TGACTGGCCT CTGTGTTTGT
    AAAGATGGCT GGATGGGACC CAACTGCGCA AAAGCTTGTG TGGCTGGACG ATGGGGGCCG
    AACTGTGCCA ACCAGTGTGA CTGCAGGAGC AGCGTGGGCA GCTGTGACCC TGTGATTGGC
    CTGTGTCATT GTGAAGCGGG CTACATGGGA CCACGCTGTG ATCAAAAGTG TCCTCAGGGT
    TTCTTCGGCC AGAGCTGCCA GCACAGATGC CAGTGTGAGA ATGGCGCAGT GTGTGAGCAT
    GTGAGCGGTG CCTGCACTTG TTTGGCTGGC TGGACTGGGA CGTATTGTGA AAAACCTTGT
    CCTGAGGGCT TTCATGGCTT GGAGTGCCAG CAGAAGTGCC AGTGCTTGAA TGGTGGCCGT
    TGTCATCCCG TGACTGGAGG CTGCCAGTGT CCATCTGGTT GGGTCGGGCC AGTTTGCAAT
    GCCACGTGTG AAGCTGGCAC CTTTGGGCCG GCCTGCAGTC AAACCTGTAG ATGCCACAAT
    AATGGCACCT GTGACCCTGC TGATGGGCGA TGTGAGTGTG GAGCAGGATG GACAGGCAGC
    AGCTGTGAAC AAGAGTGCTC AGCTGGATTG TATGGTGTGG GCTGCAACCA GCATTGCCTG
    TGTCAGAATG GAGGGTCGTG TGACCGAATC AGTGGCCACT GCACATGTGT CAGTGGGTGG
    ACAGGAGTGG CCTGTGAATT GGAATGTGCT GCGGGACACT TTGGCATGGA CTGTCAGCAC
    AAGTGTGAGT GTGTAAATGG CGGCGTGTGT GACCGTCACA GTGGCAGGTG TTTGTGCCCA
    GCAGGTTGGA CAGGCACGCA CTGTGAGACA GCATGCACTC AAGGTTTTTA TGGTCCGGGC
    TGTAAGGAGC GATGCCGGTG TGATCATAAC GCCGCATGCC ACCACATCAC TGGCACCTGC
    TTGTGTCCCG CTGGCTGGAG AGGGACTCAC TGCGAAAAGG TTTGTTTACC TGGTTCCTAT
    GGGAAGCGTT GTGCTCATCG ATGCCAGTGT CCTCATGGAA CCTCCTGTAA CCATGTGACT
    GGACAGTGTG GTTGCCCAGC GGGATTAACT GGTAGTGGCT GTGAGCGAAT GTGCCAGTCA
    GGTACGTTTG GGGTAAACTG TAATCAGGTG TGCCAGTGCT CTGAGCTCAA CCAGCTCTGC
    CACCCCGTCA CTGGTGTCTG TTACTGTGCT CCCGGATACT ATGGCAGCAA GTGTGAAACA
    GAATGTGAAG CGGGCCGTTA TGGACCCAAC TGTGAGCAGG AATGCCAGTG TCTGAATAAC
    GGTGTATGTA AAAGCAGCAC TGGCACCTGC AGCTGCCCAC CGGGATTCAT CGGACCAAAC
    TGCAACATCA CATGTCCATT AGGGCGTTAT GGTCAGGACT GTGCTGGAGT GTCTCTGTGT
    GGTGAAGGGG CTCGCAGTGA CCCTCTCACA GGCAGATGTG TGTGCAGGGC TGGTCAGCGT
    GGACGGGACT GTGGGAAAGG CTGTGCTGAG GGCTGGTTTG GAGAGAATTG TGATCAGCGC
    TGTAACTGCA GCAATGGCGG AGTGTGTGAC GCTCTTACAG GACACTGCAG CTGTACACTG
    GGCTGGACAG GAGACACCTG TGAGGAAGTG TGTCCCAGAG GACGGTATGG TTCAGACTGC
    AGTGAAGTGT GTGACTGTAA GAATAACAGC ACATGCGACC GAGTGACTGG CGTCTGTCTC
    TGCTCTCCAG GTTTCTACGG ACATCTATGT CAACATGCAT GTCCTGTTGG CTTCCATGGT
    CATCGTTGCC AGCTTCTGTG TGACTGTGGT GCAAATGCAC CTTGTGACCC TGAAACTGGC
    CGATGCCTCT GTCCTGCAGG ATACCAGGGA CTCCGCTGTG AGAGAGAGTG TGAATTGGGC
    ACATTTGGGG CTGATTGTGT CCACAGATGT GACTGTGATG GAGACACCCC ATGTGACCCT
    ATTAATGGCA GGTGTTTGTG TGCACCTGGA AAAACAGGCT CTCGCTGTGA CATAGGCTGT
    GGAGTGAACC GATTTGGACC CGACTGCTCA CAGAGATGTG AATGTCTGAA CGGAGGTCAG
    TGTGATCCCC GAAACGGCCG ATGCACGTGT CTGAACGGCT GGATCGGGCC CAGCTGTCAA
    CAAGGGCTCC TGTCTGCTAG CAAAAGTAAA AGGAAGGACT CGTCAGTATA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa44516
    Clone ID Related Accession (Same CDS sequence) XM_009296882.2 , XM_009296882.3
    Accession Version XM_009296882.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4371bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product multiple epidermal growth factor-like domains protein 6 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007134.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009296882.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    ATGTTATCCG ATTGGTTGAG GTTGGTGCTT GTGTTTACCT TCTGGACTCA GCTGGTACCA 
    AGTTTGGCTG CTGGAAATCC ACATTACCGC TACTATCACA GAAACTGCTA CACCTGCTTC
    AATGGATATC ATGCGTACAA CACAGGACAC CAGGGCGCAG ATATTGATGA GTGCCAGGTT
    CACAACGGAG GCTGCCAGCA CAGATGTGTG AACACTAGAG GCTCGTACTA CTGCGAGTGC
    AATCCAGGCT TTCGGCTCCA CGTTGATGGC CGCACCTGCA TAGCTGTTTT ATCCTGTTCG
    GTAAATAATG GAGGTTGTGA GCATGAATGT GTCCAGCTTT CTCCAGTTCA CTTCCAGTGC
    CGCTGCAGAC GTGGATACCA GCTTACAGCA GATGGCAAGC GCTGTAGATT GAGGAACCCA
    TGTGTGGAGA AGAATGGCGG CTGTGTGCAT TTATGTCGCA GTGATGGTGG TCGGGTTGTG
    TGCAGCTGTC ACCAAGGCTT CATGCTAGCA GTGGACCAGA AGAGCTGTGA AGATGTCAAT
    GAGTGTGAAA CAGGACTGGC AGCATGTGCA CACAGTTGCA GGAACACTCC AGGATCGTTT
    TCCTGCATCT GCAATCCAGG ATACGAGCTG GGCTCTGATG GAAGGAAATG CTACCGTATT
    GAAATGGAGA TTGTCAATAC CTGTGACTCT GATAATGGTG GCTGTTCACA TCGCTGTCAG
    CATGGTGTTC TTGGGCCTGT CTGCAGCTGT AATCATGGTT ATCAGCTCGC CGAAGACCTC
    AAAACCTGTT TGGATCTGGA GGAATGTGAG GAAGGAACGT CATGCTGTGA GCAGGATTGC
    ACAAACTACC CTGGAGGGTA TGAATGCTAC TGCAGGGCAG GTTACAGACT CAATGCAGAT
    GGATGCGGAT GTGACGATGT GGACGAGTGT GCGTCTGATA GAGGAGGCTG TGAACACACT
    TGTCAGAATA CGGCTGGCTC ATATCACTGC TTCTGTCGGT TTGGATATCG ACTTGGTGAG
    GACAGACACT CCTGCATTTC TCTCCAGCGA TCGGTTGAGG AATTGCCCGG TCCAGTGGTG
    GTTGAGCGTC CTCTCCTTCA TCTCGCTCTC CTCCAGGATT ACCCACAACC CCTGGAGCGC
    TATCATGACT ATGAGGAGGA CAGTTTTGGA GAGCTGCGGG CAGAGAGCAC CTTCGCTGAG
    AAGTTTGTGT GTTTGAATGG GACGTTTGGC CATGACTGCA GCCTGACGTG TGATGATTGC
    TCAAATGGAG GATATTGTAA TGCTGAAGGA GATGCTTGTG ACTGTCCAGA TGGGTGGACG
    GGGATCCTTT GTAACCAAAC CTGTCCAGAG GGAACATTTG GTAAAAACTG CTCCTTCACT
    TGCAAGTGCA GAAACGGGGG CACCTGTGAC CCGATTAGTG GGAAGTGCCG CTGCCCACCT
    GGAGTCAATG GAGACCTGTG CCAGGACGGC TGTCCGAAGG GAATGTATGG AAAGCACTGC
    AACAAGAAAT GTAACTGTGC TAATAACGGC CGCTGCCATC GCACATATGG AGCCTGTCTG
    TGTGATCCAG GACTCTACGG CAGGTTCTGT CACCTCCCTT GCCCCAAGTG GTCGTATGGA
    TCTGGCTGCT CATCCGAGTG TCAGTGTGTG CAACAAAACA CGCTTGAGTG CCATCGACGT
    CATGGAACGT GTGTGTGCAA ACCTGGCTAT CAGGGGAAAA CCTGCAACCG CGAGTGTGAT
    AGTGGATTTT ATGGTCCAGG ATGTAGATTA AAATGCCAGT GTCCAGCGGG AGTCTCTTGC
    CATCCCATGA GTGGGCAATG TCAGCGTCAG TGCCCAGCAG GCCTACATGG GGAGCACTGT
    GACCGAGAGT GCCCCATGGG ACAGTTTGGA GTTGGATGCA CACAGTTTTG TGACTGTAAG
    GGAGCTCCAT GTAACTCAGT GACCGGGAAA TGCCACTGTC CTGCAGGGCA GACAGGACAA
    CACTGTGAAA AAGAGTGTGA AGAGGGCCGT TGGGGTCTTG GCTGTGCAGA ATCATGTCCT
    TCCTGTGACA ATGGTGGAGT ATGTGATAGA CAAAACGGCA CCTGTGTTTG TTCGCCGGGA
    TTCATGGGAA ATCTCTGTCA GAATGTCTGT CCAGATGGGC ACTTTGGCCT GAGCTGCCTG
    TTGCGCTGCT CCTGTCAAAA CGAAGGGAAA TGCCACCATG TGACTGGCCT CTGTGTTTGT
    AAAGATGGCT GGATGGGACC CAACTGCGCA AAAGCTTGTG TGGCTGGACG ATGGGGGCCG
    AACTGTGCCA ACCAGTGTGA CTGCAGGAGC AGCGTGGGCA GCTGTGACCC TGTGATTGGC
    CTGTGTCATT GTGAAGCGGG CTACATGGGA CCACGCTGTG ATCAAAAGTG TCCTCAGGGT
    TTCTTCGGCC AGAGCTGCCA GCACAGATGC CAGTGTGAGA ATGGCGCAGT GTGTGAGCAT
    GTGAGCGGTG CCTGCACTTG TTTGGCTGGC TGGACTGGGA CGTATTGTGA AAAACCTTGT
    CCTGAGGGCT TTCATGGCTT GGAGTGCCAG CAGAAGTGCC AGTGCTTGAA TGGTGGCCGT
    TGTCATCCCG TGACTGGAGG CTGCCAGTGT CCATCTGGTT GGGTCGGGCC AGTTTGCAAT
    GCCACGTGTG AAGCTGGCAC CTTTGGGCCG GCCTGCAGTC AAACCTGTAG ATGCCACAAT
    AATGGCACCT GTGACCCTGC TGATGGGCGA TGTGAGTGTG GAGCAGGATG GACAGGCAGC
    AGCTGTGAAC AAGAGTGCTC AGCTGGATTG TATGGTGTGG GCTGCAACCA GCATTGCCTG
    TGTCAGAATG GAGGGTCGTG TGACCGAATC AGTGGCCACT GCACATGTGT CAGTGGGTGG
    ACAGGAGTGG CCTGTGAATT GGAATGTGCT GCGGGACACT TTGGCATGGA CTGTCAGCAC
    AAGTGTGAGT GTGTAAATGG CGGCGTGTGT GACCGTCACA GTGGCAGGTG TTTGTGCCCA
    GCAGGTTGGA CAGGCACGCA CTGTGAGACA GCATGCACTC AAGGTTTTTA TGGTCCGGGC
    TGTAAGGAGC GATGCCGGTG TGATCATAAC GCCGCATGCC ACCACATCAC TGGCACCTGC
    TTGTGTCCCG CTGGCTGGAG AGGGACTCAC TGCGAAAAGG TTTGTTTACC TGGTTCCTAT
    GGGAAGCGTT GTGCTCATCG ATGCCAGTGT CCTCATGGAA CCTCCTGTAA CCATGTGACT
    GGACAGTGTG GTTGCCCAGC GGGATTAACT GGTAGTGGCT GTGAGCGAAT GTGCCAGTCA
    GGTACGTTTG GGGTAAACTG TAATCAGGTG TGCCAGTGCT CTGAGCTCAA CCAGCTCTGC
    CACCCCGTCA CTGGTGTCTG TTACTGTGCT CCCGGATACT ATGGCAGCAA GTGTGAAACA
    GAATGTGAAG CGGGCCGTTA TGGACCCAAC TGTGAGCAGG AATGCCAGTG TCTGAATAAC
    GGTGTATGTA AAAGCAGCAC TGGCACCTGC AGCTGCCCAC CGGGATTCAT CGGACCAAAC
    TGCAACATCA CATGTCCATT AGGGCGTTAT GGTCAGGACT GTGCTGGAGT GTCTCTGTGT
    GGTGAAGGGG CTCGCAGTGA CCCTCTCACA GGCAGATGTG TGTGCAGGGC TGGTCAGCGT
    GGACGGGACT GTGGGAAAGG CTGTGCTGAG GGCTGGTTTG GAGAGAATTG TGATCAGCGC
    TGTAACTGCA GCAATGGCGG AGTGTGTGAC GCTCTTACAG GACACTGCAG CTGTACACTG
    GGCTGGACAG GAGACACCTG TGAGGAAGTG TGTCCCAGAG GACGGTATGG TTCAGACTGC
    AGTGAAGTGT GTGACTGTAA GAATAACAGC ACATGCGACC GAGTGACTGG CGTCTGTCTC
    TGCTCTCCAG GTTTCTACGG ACATCTATGT CAACATGCAT GTCCTGTTGG CTTCCATGGT
    CATCGTTGCC AGCTTCTGTG TGACTGTGGT GCAAATGCAC CTTGTGACCC TGAAACTGGC
    CGATGCCTCT GTCCTGCAGG ATACCAGGGA CTCCGCTGTG AGAGAGAGTG TGAATTGGGC
    ACATTTGGGG CTGATTGTGT CCACAGATGT GACTGTGATG GAGACACCCC ATGTGACCCT
    ATTAATGGCA GGTGTTTGTG TGCACCTGGA AAAACAGGCT CTCGCTGTGA CATAGGCTGT
    GGAGTGAACC GATTTGGACC CGACTGCTCA CAGAGATGTG AATGTCTGAA CGGAGGTCAG
    TGTGATCCCC GAAACGGCCG ATGCACGTGT CTGAACGGCT GGATCGGGCC CAGCTGTCAA
    CAAGGGCTCC TGTCTGCTAG CAAAAGTAAA AGGAAGGACT CGTCAGTATA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_009295157.1
    CDS243..4613
    Translation

    Target ORF information:

    RefSeq Version XM_009296882.3
    Organism Danio rerio(zebrafish)
    Definition Danio rerio multiple EGF-like-domains 6a (megf6a), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_009296882.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    ATGTTATCCG ATTGGTTGAG GTTGGTGCTT GTGTTTACCT TCTGGACTCA GCTGGTACCA 
    AGTTTGGCTG CTGGAAATCC ACATTACCGC TACTATCACA GAAACTGCTA CACCTGCTTC
    AATGGATATC ATGCGTACAA CACAGGACAC CAGGGCGCAG ATATTGATGA GTGCCAGGTT
    CACAACGGAG GCTGCCAGCA CAGATGTGTG AACACTAGAG GCTCGTACTA CTGCGAGTGC
    AATCCAGGCT TTCGGCTCCA CGTTGATGGC CGCACCTGCA TAGCTGTTTT ATCCTGTTCG
    GTAAATAATG GAGGTTGTGA GCATGAATGT GTCCAGCTTT CTCCAGTTCA CTTCCAGTGC
    CGCTGCAGAC GTGGATACCA GCTTACAGCA GATGGCAAGC GCTGTAGATT GAGGAACCCA
    TGTGTGGAGA AGAATGGCGG CTGTGTGCAT TTATGTCGCA GTGATGGTGG TCGGGTTGTG
    TGCAGCTGTC ACCAAGGCTT CATGCTAGCA GTGGACCAGA AGAGCTGTGA AGATGTCAAT
    GAGTGTGAAA CAGGACTGGC AGCATGTGCA CACAGTTGCA GGAACACTCC AGGATCGTTT
    TCCTGCATCT GCAATCCAGG ATACGAGCTG GGCTCTGATG GAAGGAAATG CTACCGTATT
    GAAATGGAGA TTGTCAATAC CTGTGACTCT GATAATGGTG GCTGTTCACA TCGCTGTCAG
    CATGGTGTTC TTGGGCCTGT CTGCAGCTGT AATCATGGTT ATCAGCTCGC CGAAGACCTC
    AAAACCTGTT TGGATCTGGA GGAATGTGAG GAAGGAACGT CATGCTGTGA GCAGGATTGC
    ACAAACTACC CTGGAGGGTA TGAATGCTAC TGCAGGGCAG GTTACAGACT CAATGCAGAT
    GGATGCGGAT GTGACGATGT GGACGAGTGT GCGTCTGATA GAGGAGGCTG TGAACACACT
    TGTCAGAATA CGGCTGGCTC ATATCACTGC TTCTGTCGGT TTGGATATCG ACTTGGTGAG
    GACAGACACT CCTGCATTTC TCTCCAGCGA TCGGTTGAGG AATTGCCCGG TCCAGTGGTG
    GTTGAGCGTC CTCTCCTTCA TCTCGCTCTC CTCCAGGATT ACCCACAACC CCTGGAGCGC
    TATCATGACT ATGAGGAGGA CAGTTTTGGA GAGCTGCGGG CAGAGAGCAC CTTCGCTGAG
    AAGTTTGTGT GTTTGAATGG GACGTTTGGC CATGACTGCA GCCTGACGTG TGATGATTGC
    TCAAATGGAG GATATTGTAA TGCTGAAGGA GATGCTTGTG ACTGTCCAGA TGGGTGGACG
    GGGATCCTTT GTAACCAAAC CTGTCCAGAG GGAACATTTG GTAAAAACTG CTCCTTCACT
    TGCAAGTGCA GAAACGGGGG CACCTGTGAC CCGATTAGTG GGAAGTGCCG CTGCCCACCT
    GGAGTCAATG GAGACCTGTG CCAGGACGGC TGTCCGAAGG GAATGTATGG AAAGCACTGC
    AACAAGAAAT GTAACTGTGC TAATAACGGC CGCTGCCATC GCACATATGG AGCCTGTCTG
    TGTGATCCAG GACTCTACGG CAGGTTCTGT CACCTCCCTT GCCCCAAGTG GTCGTATGGA
    TCTGGCTGCT CATCCGAGTG TCAGTGTGTG CAACAAAACA CGCTTGAGTG CCATCGACGT
    CATGGAACGT GTGTGTGCAA ACCTGGCTAT CAGGGGAAAA CCTGCAACCG CGAGTGTGAT
    AGTGGATTTT ATGGTCCAGG ATGTAGATTA AAATGCCAGT GTCCAGCGGG AGTCTCTTGC
    CATCCCATGA GTGGGCAATG TCAGCGTCAG TGCCCAGCAG GCCTACATGG GGAGCACTGT
    GACCGAGAGT GCCCCATGGG ACAGTTTGGA GTTGGATGCA CACAGTTTTG TGACTGTAAG
    GGAGCTCCAT GTAACTCAGT GACCGGGAAA TGCCACTGTC CTGCAGGGCA GACAGGACAA
    CACTGTGAAA AAGAGTGTGA AGAGGGCCGT TGGGGTCTTG GCTGTGCAGA ATCATGTCCT
    TCCTGTGACA ATGGTGGAGT ATGTGATAGA CAAAACGGCA CCTGTGTTTG TTCGCCGGGA
    TTCATGGGAA ATCTCTGTCA GAATGTCTGT CCAGATGGGC ACTTTGGCCT GAGCTGCCTG
    TTGCGCTGCT CCTGTCAAAA CGAAGGGAAA TGCCACCATG TGACTGGCCT CTGTGTTTGT
    AAAGATGGCT GGATGGGACC CAACTGCGCA AAAGCTTGTG TGGCTGGACG ATGGGGGCCG
    AACTGTGCCA ACCAGTGTGA CTGCAGGAGC AGCGTGGGCA GCTGTGACCC TGTGATTGGC
    CTGTGTCATT GTGAAGCGGG CTACATGGGA CCACGCTGTG ATCAAAAGTG TCCTCAGGGT
    TTCTTCGGCC AGAGCTGCCA GCACAGATGC CAGTGTGAGA ATGGCGCAGT GTGTGAGCAT
    GTGAGCGGTG CCTGCACTTG TTTGGCTGGC TGGACTGGGA CGTATTGTGA AAAACCTTGT
    CCTGAGGGCT TTCATGGCTT GGAGTGCCAG CAGAAGTGCC AGTGCTTGAA TGGTGGCCGT
    TGTCATCCCG TGACTGGAGG CTGCCAGTGT CCATCTGGTT GGGTCGGGCC AGTTTGCAAT
    GCCACGTGTG AAGCTGGCAC CTTTGGGCCG GCCTGCAGTC AAACCTGTAG ATGCCACAAT
    AATGGCACCT GTGACCCTGC TGATGGGCGA TGTGAGTGTG GAGCAGGATG GACAGGCAGC
    AGCTGTGAAC AAGAGTGCTC AGCTGGATTG TATGGTGTGG GCTGCAACCA GCATTGCCTG
    TGTCAGAATG GAGGGTCGTG TGACCGAATC AGTGGCCACT GCACATGTGT CAGTGGGTGG
    ACAGGAGTGG CCTGTGAATT GGAATGTGCT GCGGGACACT TTGGCATGGA CTGTCAGCAC
    AAGTGTGAGT GTGTAAATGG CGGCGTGTGT GACCGTCACA GTGGCAGGTG TTTGTGCCCA
    GCAGGTTGGA CAGGCACGCA CTGTGAGACA GCATGCACTC AAGGTTTTTA TGGTCCGGGC
    TGTAAGGAGC GATGCCGGTG TGATCATAAC GCCGCATGCC ACCACATCAC TGGCACCTGC
    TTGTGTCCCG CTGGCTGGAG AGGGACTCAC TGCGAAAAGG TTTGTTTACC TGGTTCCTAT
    GGGAAGCGTT GTGCTCATCG ATGCCAGTGT CCTCATGGAA CCTCCTGTAA CCATGTGACT
    GGACAGTGTG GTTGCCCAGC GGGATTAACT GGTAGTGGCT GTGAGCGAAT GTGCCAGTCA
    GGTACGTTTG GGGTAAACTG TAATCAGGTG TGCCAGTGCT CTGAGCTCAA CCAGCTCTGC
    CACCCCGTCA CTGGTGTCTG TTACTGTGCT CCCGGATACT ATGGCAGCAA GTGTGAAACA
    GAATGTGAAG CGGGCCGTTA TGGACCCAAC TGTGAGCAGG AATGCCAGTG TCTGAATAAC
    GGTGTATGTA AAAGCAGCAC TGGCACCTGC AGCTGCCCAC CGGGATTCAT CGGACCAAAC
    TGCAACATCA CATGTCCATT AGGGCGTTAT GGTCAGGACT GTGCTGGAGT GTCTCTGTGT
    GGTGAAGGGG CTCGCAGTGA CCCTCTCACA GGCAGATGTG TGTGCAGGGC TGGTCAGCGT
    GGACGGGACT GTGGGAAAGG CTGTGCTGAG GGCTGGTTTG GAGAGAATTG TGATCAGCGC
    TGTAACTGCA GCAATGGCGG AGTGTGTGAC GCTCTTACAG GACACTGCAG CTGTACACTG
    GGCTGGACAG GAGACACCTG TGAGGAAGTG TGTCCCAGAG GACGGTATGG TTCAGACTGC
    AGTGAAGTGT GTGACTGTAA GAATAACAGC ACATGCGACC GAGTGACTGG CGTCTGTCTC
    TGCTCTCCAG GTTTCTACGG ACATCTATGT CAACATGCAT GTCCTGTTGG CTTCCATGGT
    CATCGTTGCC AGCTTCTGTG TGACTGTGGT GCAAATGCAC CTTGTGACCC TGAAACTGGC
    CGATGCCTCT GTCCTGCAGG ATACCAGGGA CTCCGCTGTG AGAGAGAGTG TGAATTGGGC
    ACATTTGGGG CTGATTGTGT CCACAGATGT GACTGTGATG GAGACACCCC ATGTGACCCT
    ATTAATGGCA GGTGTTTGTG TGCACCTGGA AAAACAGGCT CTCGCTGTGA CATAGGCTGT
    GGAGTGAACC GATTTGGACC CGACTGCTCA CAGAGATGTG AATGTCTGAA CGGAGGTCAG
    TGTGATCCCC GAAACGGCCG ATGCACGTGT CTGAACGGCT GGATCGGGCC CAGCTGTCAA
    CAAGGGCTCC TGTCTGCTAG CAAAAGTAAA AGGAAGGACT CGTCAGTATA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

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