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necab2 cDNA ORF clone, Danio rerio(zebrafish)

Summary

Gene Symbol necab2
Entrez Gene ID 565258
Full Name N-terminal EF-hand calcium binding protein 2
Synonyms im:6912315,zgc:112232
General protein information
Preferred Names

N-terminal EF-hand calcium binding protein 2

Names

N-terminal EF-hand calcium-binding protein 2
Gene Type protein-coding
Organism Danio rerio(zebrafish)

Reference Sequences (RefSeq)

mRNA and Protein(s)

mRNA Protein Name
XM_009293572.2 XP_009291847.1 N-terminal EF-hand calcium-binding protein 2 isoform X1
XM_009293572.3 XP_009291847.1 N-terminal EF-hand calcium-binding protein 2 isoform X1
NM_001328213.1 NP_001315142.1 N-terminal EF-hand calcium-binding protein 2

Pathways from BioSystems


Homologies


Homo sapiens (human) NECAB2 NP_061938.2
Bos taurus (cattle) NECAB2 NP_001092447.1
Danio rerio (zebrafish) necab2 NP_001025366.1
Gallus gallus (chicken) NECAB2 XP_414072.1
Xenopus tropicalis (tropical clawed frog) LOC101731915 XP_004913648.1
Pan troglodytes (chimpanzee) NECAB2 XP_001150555.1
LOC718169 XP_001110489.2
Mus musculus (house mouse) Necab2 NP_473436.1
Rattus norvegicus (Norway rat) Necab2 NP_596906.1
Canis lupus familiaris (dog) NECAB2 XP_005620942.1

Gene Ontology


Bibliography

Related articles in PubMed

Evolution of complexity in the zebrafish synapse proteome.
Bay?s ?, Collins MO, Reig-Viader R, Gou G, Goulding D, Izquierdo A, Choudhary JS, Emes RD, Grant SG
Nature communications814613(2017 Mar)

Identification of p53-target genes in Danio rerio.
Mandriani B, Castellana S, Rinaldi C, Manzoni M, Venuto S, Rodriguez-Aznar E, Galceran J, Nieto MA, Borsani G, Monti E, Mazza T, Merla G, Micale L
Scientific reports632474(2016 Sep)

Effects of chronic tramadol exposure on the zebrafish brain: a proteomic study.
Zhuo HQ, Huang L, Huang HQ, Cai Z
Journal of proteomics75(11)3351-64(2012 Jun)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following necab2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the necab2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
ODa23281 NM_001328213.1
Latest version!		 Danio rerio N-terminal EF-hand calcium binding protein 2 (necab2), mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $258.30
$369.00
ODa44556 XM_009293572.3
Latest version!		 Danio rerio N-terminal EF-hand calcium binding protein 2 (necab2), transcript variant X1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $258.30
$369.00
View Old Accession Versions >>
ODa44556 XM_009293572.2 Danio rerio N-terminal EF-hand calcium binding protein 2 (necab2), transcript variant X1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $258.30
$369.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)


CloneID ODa23281
Clone ID Related Accession (Same CDS sequence) NM_001328213.1
Accession Version NM_001328213.1 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 1287bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2019-01-26
Organism Danio rerio(zebrafish)
Product N-terminal EF-hand calcium-binding protein 2
Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from CT025853.14 and BX908396.13. On Jun 9, 2016 this sequence version replaced XM_005159117.2. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## Transcript exon combination :: GFIL01018930.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2168446, SAMEA2168447 [ECO:0000348] ##Evidence-Data-END## 

1
61
121
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241
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361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
ATGTGTGAAC AAGCAGCGCG CTACTGCAGG GACGGCGTCC ACAAACTGCT CCACAAAGAA 
CAAGCAAAAC TCCGGGCACA GGACAGCAAA GACCAGCCCA CACCCGGGGC TCAGGACAGC 
GAGACAGCGA CGGCTGCACA GTCCGGAAAC AGCGGCGCAC AGCCCGGTGG AATAGACGGG 
CTCGTCCGCT GGATAGTCAC CAAGATGAGC GACAATAAAA GCATCTCTAC CCGGGAATAC 
GCGAAGGAGG AGGCGGCGGT GGCTCAGGAG CAGTTCTTCG CGCCGGAGCC TCGAAAGGGC 
ATCTCAGTCA TTTTGGATAT TTTCCGGAGA GCTGATAAGG ATGATGATGG AAAGCTTTCC 
CTTGATGAGT TCCAGGCTTT CTTCTCTGAT GGGACTCTGA ACGAAGAGGA GCTGGAAAAG 
CTGTTTCACA CCATTGACTC TGATAACACC AGTAATGTTG ACACCAAGGA ACTGTGCGAC 
TACTTTGCCA ACCACATGGG GGATTATGAA GGAGTGCTGG CCTCCTTAGA GACACTCAAC 
CTGGCCATTC TCAAAGCCAT GGACTTCACC AAGAAGGTGT ATGAACGTGG AACTAATGTT 
GAGCAGTTTG TTACCCGCTT CCTCTTGAAG GAATCAGCCA ATCAGATCCA GTCTCTGCTG 
AGCTCTGTAG AAAGTGCTGT CGATGCCATT GATGAGCAGC ATTCTTCAGT GGGATCTTAC 
CCCAGTAAGA CCAGCCCACG CGTCCCAGAT CGACGCTATG AGGCACCGGT GCCAGATTAT 
CCTCCCAACA ACAGAGTCAG TGCAAAGGAG AGCATGAGGA ACATCAACAC AGGTGCAGGG 
GCTGTGGAGG TGAGAAAAGA AGGACTTGAG GCACAGATCA ATCGACTGGC TGAGCTGATT 
GGACGTCTAG AGAACAAGAC AGTTTGGTTT GACCTACATC AGCGACTAAC AGACACTGAT 
GGCACAACTA GCGCATCCCT GTACCTGATC CGCCAGGAGA TGAAAGTTTC CCAGAAGCAG 
TTGGGTGATT TCTGTGAGGC CCTGAAACAA TACCTGAAGA ATGTGTCTGC TCAGAGAGAC 
TGTTTCCATG TGACGGCTGT CAGGCTGCCT GATGGCCTTT CCTTTGTGGT GTATGAGTTC 
TGGGATGGAG AGGAGGAGTG GAAGAGGCAT CTTCTGACTG CAGCCTGCAA GGCGTTCCAG 
CATGTGAAAG TGGATACTCT GTGTCAGCCT GAGGCGGTGT CCACTGTCGC TGTGCCCGCC 
GCCTGGTGCA GCCTGAACAG GGACTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001315142.1
CDS15..1301
Translation

Target ORF information:

RefSeq Version NM_001328213.1
Organism Danio rerio(zebrafish)
Definition Danio rerio N-terminal EF-hand calcium binding protein 2 (necab2), mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 NM_001328213.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
ATGTGTGAAC AAGCAGCGCG CTACTGCAGG GACGGCGTCC ACAAACTGCT CCACAAAGAA 
CAAGCAAAAC TCCGGGCACA GGACAGCAAA GACCAGCCCA CACCCGGGGC TCAGGACAGC 
GAGACAGCGA CGGCTGCACA GTCCGGAAAC AGCGGCGCAC AGCCCGGTGG AATAGACGGG 
CTCGTCCGCT GGATAGTCAC CAAGATGAGC GACAATAAAA GCATCTCTAC CCGGGAATAC 
GCGAAGGAGG AGGCGGCGGT GGCTCAGGAG CAGTTCTTCG CGCCGGAGCC TCGAAAGGGC 
ATCTCAGTCA TTTTGGATAT TTTCCGGAGA GCTGATAAGG ATGATGATGG AAAGCTTTCC 
CTTGATGAGT TCCAGGCTTT CTTCTCTGAT GGGACTCTGA ACGAAGAGGA GCTGGAAAAG 
CTGTTTCACA CCATTGACTC TGATAACACC AGTAATGTTG ACACCAAGGA ACTGTGCGAC 
TACTTTGCCA ACCACATGGG GGATTATGAA GGAGTGCTGG CCTCCTTAGA GACACTCAAC 
CTGGCCATTC TCAAAGCCAT GGACTTCACC AAGAAGGTGT ATGAACGTGG AACTAATGTT 
GAGCAGTTTG TTACCCGCTT CCTCTTGAAG GAATCAGCCA ATCAGATCCA GTCTCTGCTG 
AGCTCTGTAG AAAGTGCTGT CGATGCCATT GATGAGCAGC ATTCTTCAGT GGGATCTTAC 
CCCAGTAAGA CCAGCCCACG CGTCCCAGAT CGACGCTATG AGGCACCGGT GCCAGATTAT 
CCTCCCAACA ACAGAGTCAG TGCAAAGGAG AGCATGAGGA ACATCAACAC AGGTGCAGGG 
GCTGTGGAGG TGAGAAAAGA AGGACTTGAG GCACAGATCA ATCGACTGGC TGAGCTGATT 
GGACGTCTAG AGAACAAGAC AGTTTGGTTT GACCTACATC AGCGACTAAC AGACACTGAT 
GGCACAACTA GCGCATCCCT GTACCTGATC CGCCAGGAGA TGAAAGTTTC CCAGAAGCAG 
TTGGGTGATT TCTGTGAGGC CCTGAAACAA TACCTGAAGA ATGTGTCTGC TCAGAGAGAC 
TGTTTCCATG TGACGGCTGT CAGGCTGCCT GATGGCCTTT CCTTTGTGGT GTATGAGTTC 
TGGGATGGAG AGGAGGAGTG GAAGAGGCAT CTTCTGACTG CAGCCTGCAA GGCGTTCCAG 
CATGTGAAAG TGGATACTCT GTGTCAGCCT GAGGCGGTGT CCACTGTCGC TGTGCCCGCC 
GCCTGGTGCA GCCTGAACAG GGACTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID ODa44556
Clone ID Related Accession (Same CDS sequence) XM_009293572.2 , XM_009293572.3
Accession Version XM_009293572.2 Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 1230bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2016-06-23
Organism Danio rerio(Zebrafish)
Product N-terminal EF-hand calcium-binding protein 2 isoform X1
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007129.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009293572.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
ATGTGTGAAC AAGCAGCGCG CTACTGCAGG GACGGCGTCC ACAAACTGCT CCACAAAGAA 
CAAGCAAAAC TCCGGGCACA GGACAGCAAA GACCAGCCCA CACCCGGGGC TCAGGACAGC 
GAGACAGCGA CGGCTGCACA GTCCGGAAAC AGCGGCGCAC AGCCCGGTGG AATAGACGGG 
CTCGTCCGCT GGATAGTCAC CAAGATGAGC GACAATAAAA GCATCTCTAC CCGGGAATAC 
GCGAAGGAGG AGGCGGCGGT GGCTCAGGAG CAGTTCTTCG CGCCGGAGCC TCGAAAGGGC 
ATCTCAGTCA TTTTGGATAT TTTCCGGAGA GCTGATAAGG ATGATGATGG AAAGCTTTCC 
CTTGATGAGT TCCAGGCTTT CTTCTCTGAT GGGACTCTGA ACGAAGAGGA GCTGGAAAAG 
CTGTTTCACA CCATTGACTC TGATAACACC AGTAATGTTG ACACCAAGGA ACTGTGCGAC 
TACTTTGCCA ACCACATGGG GGATTATGAA GGAGTGCTGG CCTCCTTAGA GACACTCAAC 
CTGGCCATTC TCAAAGCCAT GGACTTCACC AAGAAGGTGT ATGAACGTGG AACTAATGTT 
GAGCAGTTTG TTACCCGCTT CCTCTTGAAG GAATCAGCCA ATCAGATCCA GTCTCTGCTG 
AGCTCTGTAG AAAGTGCTGT CGATGCCATT GATGAGCAGC ATTCTTCAGT GGGATCTTAC 
CCCAGTAAGA CCAGCCCACG CGTCCCAGAT CGACGCTATG AGGCACCGGT GCCAGATTAT 
CCTCCCAACA ACAGAGTCAG TGCAAAGGAG AGCATGAGGA ACATCAACAC AGGTGCAGGG 
GCTGTGGAGG TGAGAAAAGA AGGACTTGAG GCACAGATCA ATCGACTGGC TGAGCTGATT 
GGACGTCTAG AGAACAAGTC CCTGTACCTG ATCCGCCAGG AGATGAAAGT TTCCCAGAAG 
CAGTTGGGTG ATTTCTGTGA GGCCCTGAAA CAATACCTGA AGAATGTGTC TGCTCAGAGA 
GACTGTTTCC ATGTGACGGC TGTCAGGCTG CCTGATGGCC TTTCCTTTGT GGTGTATGAG 
TTCTGGGATG GAGAGGAGGA GTGGAAGAGG CATCTTCTGA CTGCAGCCTG CAAGGCGTTC 
CAGCATGTGA AAGTGGATAC TCTGTGTCAG CCTGAGGCGG TGTCCACTGT CGCTGTGCCC 
GCCGCCTGGT GCAGCCTGAA CAGGGACTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_009291847.1
CDS41..1270
Translation

Target ORF information:

RefSeq Version XM_009293572.2
Organism Danio rerio(Zebrafish)
Definition Danio rerio N-terminal EF-hand calcium binding protein 2 (necab2), transcript variant X1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 XM_009293572.2

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
ATGTGTGAAC AAGCAGCGCG CTACTGCAGG GACGGCGTCC ACAAACTGCT CCACAAAGAA 
CAAGCAAAAC TCCGGGCACA GGACAGCAAA GACCAGCCCA CACCCGGGGC TCAGGACAGC 
GAGACAGCGA CGGCTGCACA GTCCGGAAAC AGCGGCGCAC AGCCCGGTGG AATAGACGGG 
CTCGTCCGCT GGATAGTCAC CAAGATGAGC GACAATAAAA GCATCTCTAC CCGGGAATAC 
GCGAAGGAGG AGGCGGCGGT GGCTCAGGAG CAGTTCTTCG CGCCGGAGCC TCGAAAGGGC 
ATCTCAGTCA TTTTGGATAT TTTCCGGAGA GCTGATAAGG ATGATGATGG AAAGCTTTCC 
CTTGATGAGT TCCAGGCTTT CTTCTCTGAT GGGACTCTGA ACGAAGAGGA GCTGGAAAAG 
CTGTTTCACA CCATTGACTC TGATAACACC AGTAATGTTG ACACCAAGGA ACTGTGCGAC 
TACTTTGCCA ACCACATGGG GGATTATGAA GGAGTGCTGG CCTCCTTAGA GACACTCAAC 
CTGGCCATTC TCAAAGCCAT GGACTTCACC AAGAAGGTGT ATGAACGTGG AACTAATGTT 
GAGCAGTTTG TTACCCGCTT CCTCTTGAAG GAATCAGCCA ATCAGATCCA GTCTCTGCTG 
AGCTCTGTAG AAAGTGCTGT CGATGCCATT GATGAGCAGC ATTCTTCAGT GGGATCTTAC 
CCCAGTAAGA CCAGCCCACG CGTCCCAGAT CGACGCTATG AGGCACCGGT GCCAGATTAT 
CCTCCCAACA ACAGAGTCAG TGCAAAGGAG AGCATGAGGA ACATCAACAC AGGTGCAGGG 
GCTGTGGAGG TGAGAAAAGA AGGACTTGAG GCACAGATCA ATCGACTGGC TGAGCTGATT 
GGACGTCTAG AGAACAAGTC CCTGTACCTG ATCCGCCAGG AGATGAAAGT TTCCCAGAAG 
CAGTTGGGTG ATTTCTGTGA GGCCCTGAAA CAATACCTGA AGAATGTGTC TGCTCAGAGA 
GACTGTTTCC ATGTGACGGC TGTCAGGCTG CCTGATGGCC TTTCCTTTGT GGTGTATGAG 
TTCTGGGATG GAGAGGAGGA GTGGAAGAGG CATCTTCTGA CTGCAGCCTG CAAGGCGTTC 
CAGCATGTGA AAGTGGATAC TCTGTGTCAG CCTGAGGCGG TGTCCACTGT CGCTGTGCCC 
GCCGCCTGGT GCAGCCTGAA CAGGGACTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID ODa44556
Clone ID Related Accession (Same CDS sequence) XM_009293572.2 , XM_009293572.3
Accession Version XM_009293572.3 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 1230bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2017-06-14
Organism Danio rerio(zebrafish)
Product N-terminal EF-hand calcium-binding protein 2 isoform X1
Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007129.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009293572.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
ATGTGTGAAC AAGCAGCGCG CTACTGCAGG GACGGCGTCC ACAAACTGCT CCACAAAGAA 
CAAGCAAAAC TCCGGGCACA GGACAGCAAA GACCAGCCCA CACCCGGGGC TCAGGACAGC 
GAGACAGCGA CGGCTGCACA GTCCGGAAAC AGCGGCGCAC AGCCCGGTGG AATAGACGGG 
CTCGTCCGCT GGATAGTCAC CAAGATGAGC GACAATAAAA GCATCTCTAC CCGGGAATAC 
GCGAAGGAGG AGGCGGCGGT GGCTCAGGAG CAGTTCTTCG CGCCGGAGCC TCGAAAGGGC 
ATCTCAGTCA TTTTGGATAT TTTCCGGAGA GCTGATAAGG ATGATGATGG AAAGCTTTCC 
CTTGATGAGT TCCAGGCTTT CTTCTCTGAT GGGACTCTGA ACGAAGAGGA GCTGGAAAAG 
CTGTTTCACA CCATTGACTC TGATAACACC AGTAATGTTG ACACCAAGGA ACTGTGCGAC 
TACTTTGCCA ACCACATGGG GGATTATGAA GGAGTGCTGG CCTCCTTAGA GACACTCAAC 
CTGGCCATTC TCAAAGCCAT GGACTTCACC AAGAAGGTGT ATGAACGTGG AACTAATGTT 
GAGCAGTTTG TTACCCGCTT CCTCTTGAAG GAATCAGCCA ATCAGATCCA GTCTCTGCTG 
AGCTCTGTAG AAAGTGCTGT CGATGCCATT GATGAGCAGC ATTCTTCAGT GGGATCTTAC 
CCCAGTAAGA CCAGCCCACG CGTCCCAGAT CGACGCTATG AGGCACCGGT GCCAGATTAT 
CCTCCCAACA ACAGAGTCAG TGCAAAGGAG AGCATGAGGA ACATCAACAC AGGTGCAGGG 
GCTGTGGAGG TGAGAAAAGA AGGACTTGAG GCACAGATCA ATCGACTGGC TGAGCTGATT 
GGACGTCTAG AGAACAAGTC CCTGTACCTG ATCCGCCAGG AGATGAAAGT TTCCCAGAAG 
CAGTTGGGTG ATTTCTGTGA GGCCCTGAAA CAATACCTGA AGAATGTGTC TGCTCAGAGA 
GACTGTTTCC ATGTGACGGC TGTCAGGCTG CCTGATGGCC TTTCCTTTGT GGTGTATGAG 
TTCTGGGATG GAGAGGAGGA GTGGAAGAGG CATCTTCTGA CTGCAGCCTG CAAGGCGTTC 
CAGCATGTGA AAGTGGATAC TCTGTGTCAG CCTGAGGCGG TGTCCACTGT CGCTGTGCCC 
GCCGCCTGGT GCAGCCTGAA CAGGGACTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_009291847.1
CDS50..1279
Translation

Target ORF information:

RefSeq Version XM_009293572.3
Organism Danio rerio(zebrafish)
Definition Danio rerio N-terminal EF-hand calcium binding protein 2 (necab2), transcript variant X1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 XM_009293572.3

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
ATGTGTGAAC AAGCAGCGCG CTACTGCAGG GACGGCGTCC ACAAACTGCT CCACAAAGAA 
CAAGCAAAAC TCCGGGCACA GGACAGCAAA GACCAGCCCA CACCCGGGGC TCAGGACAGC 
GAGACAGCGA CGGCTGCACA GTCCGGAAAC AGCGGCGCAC AGCCCGGTGG AATAGACGGG 
CTCGTCCGCT GGATAGTCAC CAAGATGAGC GACAATAAAA GCATCTCTAC CCGGGAATAC 
GCGAAGGAGG AGGCGGCGGT GGCTCAGGAG CAGTTCTTCG CGCCGGAGCC TCGAAAGGGC 
ATCTCAGTCA TTTTGGATAT TTTCCGGAGA GCTGATAAGG ATGATGATGG AAAGCTTTCC 
CTTGATGAGT TCCAGGCTTT CTTCTCTGAT GGGACTCTGA ACGAAGAGGA GCTGGAAAAG 
CTGTTTCACA CCATTGACTC TGATAACACC AGTAATGTTG ACACCAAGGA ACTGTGCGAC 
TACTTTGCCA ACCACATGGG GGATTATGAA GGAGTGCTGG CCTCCTTAGA GACACTCAAC 
CTGGCCATTC TCAAAGCCAT GGACTTCACC AAGAAGGTGT ATGAACGTGG AACTAATGTT 
GAGCAGTTTG TTACCCGCTT CCTCTTGAAG GAATCAGCCA ATCAGATCCA GTCTCTGCTG 
AGCTCTGTAG AAAGTGCTGT CGATGCCATT GATGAGCAGC ATTCTTCAGT GGGATCTTAC 
CCCAGTAAGA CCAGCCCACG CGTCCCAGAT CGACGCTATG AGGCACCGGT GCCAGATTAT 
CCTCCCAACA ACAGAGTCAG TGCAAAGGAG AGCATGAGGA ACATCAACAC AGGTGCAGGG 
GCTGTGGAGG TGAGAAAAGA AGGACTTGAG GCACAGATCA ATCGACTGGC TGAGCTGATT 
GGACGTCTAG AGAACAAGTC CCTGTACCTG ATCCGCCAGG AGATGAAAGT TTCCCAGAAG 
CAGTTGGGTG ATTTCTGTGA GGCCCTGAAA CAATACCTGA AGAATGTGTC TGCTCAGAGA 
GACTGTTTCC ATGTGACGGC TGTCAGGCTG CCTGATGGCC TTTCCTTTGT GGTGTATGAG 
TTCTGGGATG GAGAGGAGGA GTGGAAGAGG CATCTTCTGA CTGCAGCCTG CAAGGCGTTC 
CAGCATGTGA AAGTGGATAC TCTGTGTCAG CCTGAGGCGG TGTCCACTGT CGCTGTGCCC 
GCCGCCTGGT GCAGCCTGAA CAGGGACTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

PubMed

book

Evolution of complexity in the zebrafish synapse proteome.
Nature communications814613(2017 Mar)
Bay?s ?,Collins MO,Reig-Viader R,Gou G,Goulding D,Izquierdo A,Choudhary JS,Emes RD,Grant SG


book

Identification of p53-target genes in Danio rerio.
Scientific reports632474(2016 Sep)
Mandriani B,Castellana S,Rinaldi C,Manzoni M,Venuto S,Rodriguez-Aznar E,Galceran J,Nieto MA,Borsani G,Monti E,Mazza T,Merla G,Micale L


book

Effects of chronic tramadol exposure on the zebrafish brain: a proteomic study.
Journal of proteomics75(11)3351-64(2012 Jun)
Zhuo HQ,Huang L,Huang HQ,Cai Z