cacna2d2b cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Gene Symbol	cacna2d2b
Entrez Gene ID	568759
Full Name	calcium channel, voltage-dependent, alpha 2/delta subunit 2b
Synonyms	si:ch211-11p18.4
General protein information	Preferred Names calcium channel, voltage-dependent, alpha 2/delta subunit 2b Names calcium channel, voltage-dependent, alpha 2/delta subunit 2
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
XM_009296111.1	XP_009294386.1	calcium channel, voltage-dependent, alpha 2/delta subunit 2 isoform X2
XM_009296112.1	XP_009294387.1	calcium channel, voltage-dependent, alpha 2/delta subunit 2 isoform X3
XM_009296112.2	XP_009294387.1	calcium channel, voltage-dependent, alpha 2/delta subunit 2 isoform X3
XM_009296110.1	XP_009294385.1	calcium channel, voltage-dependent, alpha 2/delta subunit 2 isoform X1
XM_009296110.2	XP_009294385.1	calcium channel, voltage-dependent, alpha 2/delta subunit 2 isoform X1
NM_001089420.1	NP_001082889.1	calcium channel, voltage-dependent, alpha 2/delta subunit 2
XM_009296111.2	XP_009294386.1	calcium channel, voltage-dependent, alpha 2/delta subunit 2 isoform X2

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	CACNA2D2	XP_005632558.1
Mus musculus (house mouse)	Cacna2d2	NP_001167518.1
Danio rerio (zebrafish)	LOC100333639	XP_002666248.3
Pan troglodytes (chimpanzee)	CACNA2D2	XP_003309914.1
Rattus norvegicus (Norway rat)	Cacna2d2	NP_783182.1
Gallus gallus (chicken)	CACNA2D2	XP_427707.4
Danio rerio (zebrafish)	si:ch211-11p18.4	NP_001082889.1
Homo sapiens (human)	CACNA2D2	NP_001167522.1
	CACNA2D2	XP_001090735.2
Bos taurus (cattle)	CACNA2D2	XP_002697119.3
Xenopus tropicalis (tropical clawed frog)	cacna2d2	XP_002935678.2

Gene Ontology
Bibliography

Related articles in PubMed

Evolution of complexity in the zebrafish synapse proteome.
Bay?s ?, Collins MO, Reig-Viader R, Gou G, Goulding D, Izquierdo A, Choudhary JS, Emes RD, Grant SG
Nature communications814613(2017 Mar)

Corticotropin-releasing hormone family evolution: five ancestral genes remain in some lineages.
Cardoso JC, Bergqvist CA, F?lix RC, Larhammar D
Journal of molecular endocrinology57(1)73-86(2016 07)

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
Pasquier J, Cabau C, Nguyen T, Jouanno E, Severac D, Braasch I, Journot L, Pontarotti P, Klopp C, Postlethwait JH, Guiguen Y, Bobe J
BMC genomics17368(2016 05)

RFX transcription factors are essential for hearing in mice.
Elkon R, Milon B, Morrison L, Shah M, Vijayakumar S, Racherla M, Leitch CC, Silipino L, Hadi S, Weiss-Gayet M, Barras E, Schmid CD, Ait-Lounis A, Barnes A, Song Y, Eisenman DJ, Eliyahu E, Frolenkov GI, Strome SE, Durand B, Zaghloul NA, Jones SM, Reith W, Hertzano R
Nature communications68549(2015 Oct)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following cacna2d2b gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the cacna2d2b cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODa35274	NM_001089420.1 Latest version!	Danio rerio calcium channel, voltage-dependent, alpha 2/delta subunit 2b (cacna2d2b), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
ODa26699	XM_009296110.2 Latest version!	Danio rerio calcium channel, voltage-dependent, alpha 2/delta subunit 2b (cacna2d2b), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
ODa26700	XM_009296112.2 Latest version!	Danio rerio calcium channel, voltage-dependent, alpha 2/delta subunit 2b (cacna2d2b), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
ODa45532	XM_009296111.2 Latest version!	Danio rerio calcium channel, voltage-dependent, alpha 2/delta subunit 2b (cacna2d2b), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
View Old Accession Versions >>
ODa26700	XM_009296112.1	Danio rerio calcium channel, voltage-dependent, alpha 2/delta subunit 2b (cacna2d2b), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
ODa26699	XM_009296110.1	Danio rerio calcium channel, voltage-dependent, alpha 2/delta subunit 2b (cacna2d2b), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
ODa45532	XM_009296111.1	Danio rerio calcium channel, voltage-dependent, alpha 2/delta subunit 2b (cacna2d2b), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
Hide Old Accession Versions >>

ORF Online Only Promotion

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

cacna2d2b ( NM_001089420.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_001082889.1 Danio rerio calcium channel, voltage-dependent, alpha 2/delta subunit 2b (cacna2d2b), mRNA.

Price & Availability↑

CloneID	ODa35274
Clone ID Related Accession (Same CDS sequence)	NM_001089420.1
Accession Version	NM_001089420.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3159bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-01-26
Organism	Danio rerio(zebrafish)
Product	calcium channel, voltage-dependent, alpha 2/delta subunit 2
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AL845526.25. On May 23, 2007 this sequence version replaced XM_692110.2. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMEA3505377, SAMEA4476734 [ECO:0000350] ##Evidence-Data-END##

ATGCACTGGG CTCGACGCAT TGAACAGGAG ATCGACAGAG TTTTGCAGCA TATTAGTGGC 
GCTCAGCAAC TGAAGGGGAT CTACTTGGAA AAGAAAAAGC ACTTCAGTCT GGTCAGAAAC 
AACCCTCGAG ACATTGTAGA GAAAGTAGCG ACTGACATCG AGAGACTGCT TGCCAAGAAA 
CGCAAAGCGC TGGAGAGACT GGCGAGTGAA GCGGAGCGTC TTCAGAAAGA GCATCGCTGG 
CAGGATGGAA TCAAGGACGG TGAGGACATT GACTCTCAGA TGTCCTTGAA ACTGGATTTT 
GTGTACGACC CAAGCTTCAA AAACCAAGTG AATTACTCGC ACACTGCCGT CCAGATCCCC 
ACAGACATCT ACAAAGGCGC TCCAGTGATT CTGAATGAGC TGAACTGGAC GCAGGCGCTA 
GAGCGAGTCT TCATTGAGAA CAGCAGAGAT GATCCCTCAT TGCTTTGGCA AGCATTTGGT 
AGTGCCACCG GTGTCACCAG ATATTACCCA GCTGCTCCCT GGAGAGCGCC AGATAAGATC 
GACCTTTATG ATGTACGACG AAGACCCTGG TACATTCAAG GAGCGTCGTC ACCCAAAGAC 
ATGGTCATTC TTGTAGACGT GAGCGGCAGC GTCAGCGGGC TCACATTAAA ACTGATCAAA 
GCTTCGGTCA CAGAAATGCT CGACACACTC TCCGATGATG ATTATGTCAA CGTGGCCAGG 
TTTAATGAGA AGGCAGAGGC AGTGGTGCCC TGTTTCGATC ACCTGGTTCA GGCCAATGTG 
CGCAATAAGA AGATCTTCAA AGAGGCTGTG CAGCAGATGC AGGCCAAAGG GACCACCGAT 
TACAAATCCG GCTTCCATTT CGCCTTCAAC CAGCTCCTGA ACAAGACAAA CGTTCCTCGA 
GCCAACTGTA ATAAAATCAT CATGTTGTTC ACTGACGGCG GAGAGGACCG AGCGCAGGAC 
ATCTTCGAGC AGTACAACTG GCCTAATAAG ACGGTCCGCG TGTTCACGTT CTCGGTGGGG 
CAGCACAATT ACGATGTCAC TCCGCTGCAG TGGATTGCAT GCTCTAATAA GGGATACTAT 
TTTGAGATCC GCTCCATCTG TGCTATAAGG ATTAACACCC AGGAATATCT GGATGTATTG 
GGTCGCCCGA TGGTTCTGGC AGGCAGAATC GCCAAACAAG TACAGTGGAC AAACGTCTAT 
CAGGACGCCC TGGGTCTGGG TCTGGTGGTG ACAGGGACAA TGCCGGTGTT CAACCTTACT 
GTGAACGGGG ACTCTCAGAA TCAGCTGATT TTAGGTGTGA TGGGGGTCGA CGTTCATCTG 
GACGAGCTGA AGAGACTCAC ACCACAGTAC AAGCTCGGAG CCAATGGATA CATCTTCGCC 
ATTGACCCGA ACGGATACGT GCTGATGCAT CCAAACCTGC AGCCTCGAGA AGAGGATTTA 
CCGGAGCAGG TGACTCTGGA CTTTCTGGAT GCAGAAGTGG AAGACAGCAG CAAACAGGAG 
ATTCGCCGGC AGATGATTGA CGGCCGATCG GGAGATATGA CCATTAAAAC TCTAATTAAA 
TCAATGGACG AGGCAAGTCG CTACATTGAC GACGTCACCC GGATGTACAC CTGGACGCCC 
ATCAACGACA CAGACTACAG TCTAGGTTTG GTACTGCCTC CGTACAGTGA ACATTACATC 
CAAGCTGATC TGAGTGATGT CATGCTCCAG CTGCAGTATT TACAGTCTCT GCTGCCCAAC 
TCTTTCGAGT CTGCCGGTCA TGTGTTTCTC GCTCCCAGAG ATTATTGCAG GCGCCTGCAC 
ATCTCTGAAA ACAACACACA GTTCCTGGTG GACTTCCTGT CTCTGATGGT GGAGATCTCA 
CCGGAGTCTG AAGACTGTGA TCAGGGTCTC ATTCATAACC TGATCTTGGA CTCCGGGATC 
CTCTGGCAGC TCGCCACACG CGTTTGGCAG AATAAAGACC TGGGCACGTA TGGCTTTCTG 
GCTCTGTTTG CGTCCACCAA TGGAGGCGTC ACACGTGTTT ACCCCAACAT GGCGGCTGAA 
TCATGGGATG AAGATCCTGA ACCACTAAAC TCAAACTTCT ACCGGCGCAG TCTGGATAAC 
AAAGGCTACA TCTTCAGAGC GCCGACACGA ACCTCGATGG ATGATCTGCT GATCTCAGAG 
AATGGCACTG TGGGGATTCT GGTTACCACC GCCGTAGAGG TTGCATTTGG GAGCAAAACC 
ATGAAGCCTG CAGTGGTCGG CGTTAAGCTG GATTTGGAGG CTTGGGTCGA CAAGTTCAAG 
ATCTTGGCCA GCAACGTGTC CGACAGCAGA CAGGCCGCAC ATAAGTGCGG CCCGTCCAGA 
TCCTGTGAGA TGGACTGTGA AGTGAACAGC GATGATTTGC TGTGCTATCT GATCGATGAT 
GGCGGTTTCC TGGTGATGTC CAATCAGAGA GATCACTGGA AGAAGGTTGG GCTGTTTTTC 
GGTGAGGTCG ACCCGTACCT GATGTTCGCC CTCTACAACA ACTCAATCTA CTCCCGTCAT 
CAGACCTTCC AGTATCAGTC GGCCTGCGAA CCCACAGCCA GCAGCCACAC AGGAGCCGCA 
CACAGAGGAT TCTACGTGCC CTCCATAGCG GACTTTCTCA GCCTAGCCTG GTGGACGTCT 
GCAGCCGCGT GGTCTATTTT GCAGCAGTTC TTGTATGGAG TCATGTACAG CAGCTGGTTC 
ACCACAGCAG ACGTCTGGGC TGAGAGTGCA GACAGTAAAG GGAGCAGCAG CTGTGTGACG 
GTTTACAGCC AGTTCTACTT CACCAACACC ACCAACTCCT TCAACGTCCT GCAGGACTGC 
GGCAACTGCT CCAGCATTCT AACCACAAAC TCTCTTTCTG TCAGGTTGTT CCATGCCAAG 
CGTATAGAAA ACACAAACCT TTTGTTTGTG GTGGCTGAGA CACTGCCCTG CAGCTCCTGC 
GAGATCGAGA AACTCCTGCC TGTCAGAACC AAGTCTCAAG AAGAAAACCC ATGTGATGTC 
TTAAACTCGG CCCGCTATAG GAAAGGACCT GACAAATGCT TCGACTACAA CTCAGAGGAG 
AACACATCAC AGTGTGGCAG CGCTCACTCT TTACACTGTC CGCTGCCAGC TCTGCTCTTG 
GTTCAGTTAT TTTTGATTTA CCTCATTCTA CGATCGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001082889.1
CDS	4..3162
Translation	MHWARRIEQEIDRVLQHISGAQQLKGIYLEKKKHFSLVRNNPRDIVEKVATDIERLLAKKRKALERLASEAERLQKEHRWQDGIKDGEDIDSQMSLKLDFVYDPSFKNQVNYSHTAVQIPTDIYKGAPVILNELNWTQALERVFIENSRDDPSLLWQAFGSATGVTRYYPAAPWRAPDKIDLYDVRRRPWYIQGASSPKDMVILVDVSGSVSGLTLKLIKASVTEMLDTLSDDDYVNVARFNEKAEAVVPCFDHLVQANVRNKKIFKEAVQQMQAKGTTDYKSGFHFAFNQLLNKTNVPRANCNKIIMLFTDGGEDRAQDIFEQYNWPNKTVRVFTFSVGQHNYDVTPLQWIACSNKGYYFEIRSICAIRINTQEYLDVLGRPMVLAGRIAKQVQWTNVYQDALGLGLVVTGTMPVFNLTVNGDSQNQLILGVMGVDVHLDELKRLTPQYKLGANGYIFAIDPNGYVLMHPNLQPREEDLPEQVTLDFLDAEVEDSSKQEIRRQMIDGRSGDMTIKTLIKSMDEASRYIDDVTRMYTWTPINDTDYSLGLVLPPYSEHYIQADLSDVMLQLQYLQSLLPNSFESAGHVFLAPRDYCRRLHISENNTQFLVDFLSLMVEISPESEDCDQGLIHNLILDSGILWQLATRVWQNKDLGTYGFLALFASTNGGVTRVYPNMAAESWDEDPEPLNSNFYRRSLDNKGYIFRAPTRTSMDDLLISENGTVGILVTTAVEVAFGSKTMKPAVVGVKLDLEAWVDKFKILASNVSDSRQAAHKCGPSRSCEMDCEVNSDDLLCYLIDDGGFLVMSNQRDHWKKVGLFFGEVDPYLMFALYNNSIYSRHQTFQYQSACEPTASSHTGAAHRGFYVPSIADFLSLAWWTSAAAWSILQQFLYGVMYSSWFTTADVWAESADSKGSSSCVTVYSQFYFTNTTNSFNVLQDCGNCSSILTTNSLSVRLFHAKRIENTNLLFVVAETLPCSSCEIEKLLPVRTKSQEENPCDVLNSARYRKGPDKCFDYNSEENTSQCGSAHSLHCPLPALLLVQLFLIYLILRS

Target ORF information:

RefSeq Version	NM_001089420.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio calcium channel, voltage-dependent, alpha 2/delta subunit 2b (cacna2d2b), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001089420.1

ORF Insert Sequence:

ATGCACTGGG CTCGACGCAT TGAACAGGAG ATCGACAGAG TTTTGCAGCA TATTAGTGGC 
GCTCAGCAAC TGAAGGGGAT CTACTTGGAA AAGAAAAAGC ACTTCAGTCT GGTCAGAAAC 
AACCCTCGAG ACATTGTAGA GAAAGTAGCG ACTGACATCG AGAGACTGCT TGCCAAGAAA 
CGCAAAGCGC TGGAGAGACT GGCGAGTGAA GCGGAGCGTC TTCAGAAAGA GCATCGCTGG 
CAGGATGGAA TCAAGGACGG TGAGGACATT GACTCTCAGA TGTCCTTGAA ACTGGATTTT 
GTGTACGACC CAAGCTTCAA AAACCAAGTG AATTACTCGC ACACTGCCGT CCAGATCCCC 
ACAGACATCT ACAAAGGCGC TCCAGTGATT CTGAATGAGC TGAACTGGAC GCAGGCGCTA 
GAGCGAGTCT TCATTGAGAA CAGCAGAGAT GATCCCTCAT TGCTTTGGCA AGCATTTGGT 
AGTGCCACCG GTGTCACCAG ATATTACCCA GCTGCTCCCT GGAGAGCGCC AGATAAGATC 
GACCTTTATG ATGTACGACG AAGACCCTGG TACATTCAAG GAGCGTCGTC ACCCAAAGAC 
ATGGTCATTC TTGTAGACGT GAGCGGCAGC GTCAGCGGGC TCACATTAAA ACTGATCAAA 
GCTTCGGTCA CAGAAATGCT CGACACACTC TCCGATGATG ATTATGTCAA CGTGGCCAGG 
TTTAATGAGA AGGCAGAGGC AGTGGTGCCC TGTTTCGATC ACCTGGTTCA GGCCAATGTG 
CGCAATAAGA AGATCTTCAA AGAGGCTGTG CAGCAGATGC AGGCCAAAGG GACCACCGAT 
TACAAATCCG GCTTCCATTT CGCCTTCAAC CAGCTCCTGA ACAAGACAAA CGTTCCTCGA 
GCCAACTGTA ATAAAATCAT CATGTTGTTC ACTGACGGCG GAGAGGACCG AGCGCAGGAC 
ATCTTCGAGC AGTACAACTG GCCTAATAAG ACGGTCCGCG TGTTCACGTT CTCGGTGGGG 
CAGCACAATT ACGATGTCAC TCCGCTGCAG TGGATTGCAT GCTCTAATAA GGGATACTAT 
TTTGAGATCC GCTCCATCTG TGCTATAAGG ATTAACACCC AGGAATATCT GGATGTATTG 
GGTCGCCCGA TGGTTCTGGC AGGCAGAATC GCCAAACAAG TACAGTGGAC AAACGTCTAT 
CAGGACGCCC TGGGTCTGGG TCTGGTGGTG ACAGGGACAA TGCCGGTGTT CAACCTTACT 
GTGAACGGGG ACTCTCAGAA TCAGCTGATT TTAGGTGTGA TGGGGGTCGA CGTTCATCTG 
GACGAGCTGA AGAGACTCAC ACCACAGTAC AAGCTCGGAG CCAATGGATA CATCTTCGCC 
ATTGACCCGA ACGGATACGT GCTGATGCAT CCAAACCTGC AGCCTCGAGA AGAGGATTTA 
CCGGAGCAGG TGACTCTGGA CTTTCTGGAT GCAGAAGTGG AAGACAGCAG CAAACAGGAG 
ATTCGCCGGC AGATGATTGA CGGCCGATCG GGAGATATGA CCATTAAAAC TCTAATTAAA 
TCAATGGACG AGGCAAGTCG CTACATTGAC GACGTCACCC GGATGTACAC CTGGACGCCC 
ATCAACGACA CAGACTACAG TCTAGGTTTG GTACTGCCTC CGTACAGTGA ACATTACATC 
CAAGCTGATC TGAGTGATGT CATGCTCCAG CTGCAGTATT TACAGTCTCT GCTGCCCAAC 
TCTTTCGAGT CTGCCGGTCA TGTGTTTCTC GCTCCCAGAG ATTATTGCAG GCGCCTGCAC 
ATCTCTGAAA ACAACACACA GTTCCTGGTG GACTTCCTGT CTCTGATGGT GGAGATCTCA 
CCGGAGTCTG AAGACTGTGA TCAGGGTCTC ATTCATAACC TGATCTTGGA CTCCGGGATC 
CTCTGGCAGC TCGCCACACG CGTTTGGCAG AATAAAGACC TGGGCACGTA TGGCTTTCTG 
GCTCTGTTTG CGTCCACCAA TGGAGGCGTC ACACGTGTTT ACCCCAACAT GGCGGCTGAA 
TCATGGGATG AAGATCCTGA ACCACTAAAC TCAAACTTCT ACCGGCGCAG TCTGGATAAC 
AAAGGCTACA TCTTCAGAGC GCCGACACGA ACCTCGATGG ATGATCTGCT GATCTCAGAG 
AATGGCACTG TGGGGATTCT GGTTACCACC GCCGTAGAGG TTGCATTTGG GAGCAAAACC 
ATGAAGCCTG CAGTGGTCGG CGTTAAGCTG GATTTGGAGG CTTGGGTCGA CAAGTTCAAG 
ATCTTGGCCA GCAACGTGTC CGACAGCAGA CAGGCCGCAC ATAAGTGCGG CCCGTCCAGA 
TCCTGTGAGA TGGACTGTGA AGTGAACAGC GATGATTTGC TGTGCTATCT GATCGATGAT 
GGCGGTTTCC TGGTGATGTC CAATCAGAGA GATCACTGGA AGAAGGTTGG GCTGTTTTTC 
GGTGAGGTCG ACCCGTACCT GATGTTCGCC CTCTACAACA ACTCAATCTA CTCCCGTCAT 
CAGACCTTCC AGTATCAGTC GGCCTGCGAA CCCACAGCCA GCAGCCACAC AGGAGCCGCA 
CACAGAGGAT TCTACGTGCC CTCCATAGCG GACTTTCTCA GCCTAGCCTG GTGGACGTCT 
GCAGCCGCGT GGTCTATTTT GCAGCAGTTC TTGTATGGAG TCATGTACAG CAGCTGGTTC 
ACCACAGCAG ACGTCTGGGC TGAGAGTGCA GACAGTAAAG GGAGCAGCAG CTGTGTGACG 
GTTTACAGCC AGTTCTACTT CACCAACACC ACCAACTCCT TCAACGTCCT GCAGGACTGC 
GGCAACTGCT CCAGCATTCT AACCACAAAC TCTCTTTCTG TCAGGTTGTT CCATGCCAAG 
CGTATAGAAA ACACAAACCT TTTGTTTGTG GTGGCTGAGA CACTGCCCTG CAGCTCCTGC 
GAGATCGAGA AACTCCTGCC TGTCAGAACC AAGTCTCAAG AAGAAAACCC ATGTGATGTC 
TTAAACTCGG CCCGCTATAG GAAAGGACCT GACAAATGCT TCGACTACAA CTCAGAGGAG 
AACACATCAC AGTGTGGCAG CGCTCACTCT TTACACTGTC CGCTGCCAGC TCTGCTCTTG 
GTTCAGTTAT TTTTGATTTA CCTCATTCTA CGATCGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

cacna2d2b ( XM_009296110.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009294385.1 Danio rerio calcium channel, voltage-dependent, alpha 2/delta subunit 2b (cacna2d2b), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa26699
Clone ID Related Accession (Same CDS sequence)	XM_009296110.1 , XM_009296110.2
Accession Version	XM_009296110.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3273bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	calcium channel, voltage-dependent, alpha 2/delta subunit 2 isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007133.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGAGAT GGGCTCACGC GTGTCTGCTG CTGCTGCTGA TGGTGATTCT CGGCGGATCC 
TGGACCGCAG TGAACGCGCT CACATTTCCT CAGCATTACA CGATTATGCA CTGGGCTCGA 
CGCATTGAAC AGGAGATCGA CAGAGTTTTG CAGCATATTA GTGGCGCTCA GCAACTGAAG 
GGGATCTACT TGGAAAAGAA AAAGCACTTC AGTCTGGTCA GAAACAACCC TCGAGACATT 
GTAGAGAAAG TAGCGACTGA CATCGAGAGA CTGCTTGCCA AGAAACGCAA AGCGCTGGAG 
AGACTGGCGA GTGAAGCGGA GCGTCTTCAG AAAGAGCATC GCTGGCAGGA TGGAATCAAG 
GAGGAAAATA TTGAGTATTA TAACTCTAAA GCAGAGATGG ATTATGACGG TGAGGACATT 
GACTCTCAGA TGTCCTTGAA ACTGGATTTT GTGTACGACC CAAGCTTCAA AAACCAAGTG 
AATTACTCGC ACACTGCCGT CCAGATCCCC ACAGACATCT ACAAAGGCGC TCCAGTGATT 
CTGAATGAGC TGAACTGGAC GCAGGCGCTA GAGCGAGTCT TCATTGAGAA CAGCAGAGAT 
GATCCCTCAT TGCTTTGGCA AGCATTTGGT AGTGCCACCG GTGTCACCAG ATATTACCCA 
GCTGCTCCCT GGAGAGCGCC AGATAAGATC GACCTTTATG ATGTACGACG AAGACCCTGG 
TACATTCAAG GAGCGTCGTC ACCCAAAGAC ATGGTCATTC TTGTAGACGT GAGCGGCAGC 
GTCAGCGGGC TCACATTAAA ACTGATCAAA GCTTCGGTCA CAGAAATGCT CGACACACTC 
TCCGATGATG ATTATGTCAA CGTGGCCAGG TTTAATGAGA AGGCAGAGGC AGTGGTGCCC 
TGTTTCGATC ACCTGGTTCA GGCCAATGTG CGCAATAAGA AGATCTTCAA AGAGGCTGTG 
CAGCAGATGC AGGCCAAAGG GACCACCGAT TACAAATCCG GCTTCCATTT CGCCTTCAAC 
CAGCTCCTGA ACAAGACAAA CGTTCCTCGA GCCAACTGTA ATAAAATCAT CATGTTGTTC 
ACTGACGGCG GAGAGGACCG AGCGCAGGAC ATCTTCGAGC AGTACAACTG GCCTAATAAG 
ACGGTCCGCG TGTTCACGTT CTCGGTGGGG CAGCACAATT ACGATGTCAC TCCGCTGCAG 
TGGATTGCAT GCTCTAATAA GGGATACTAT TTTGAGATCC GCTCCATCTG TGCTATAAGG 
ATTAACACCC AGGAATATCT GGATGTATTG GGTCGCCCGA TGGTTCTGGC AGGCAGAATC 
GCCAAACAAG TACAGTGGAC AAACGTCTAT CAGGACGCCC TGGGTCTGGG TCTGGTGGTG 
ACAGGGACAA TGCCGGTGTT CAACCTTACT GTGAACGGGG ACTCTCAGAA TCAGCTGATT 
TTAGGTGTGA TGGGGGTCGA CGTTCATCTG GACGAGCTGA AGAGACTCAC ACCACAGTAC 
AAGCTCGGAG CCAATGGATA CATCTTCGCC ATTGACCCGA ACGGATACGT GCTGATGCAT 
CCAAACCTGC AGCCTCGAGA AGAGGATTTA CCGGAGCAGG TGACTCTGGA CTTTCTGGAT 
GCAGAAGTGG AAGACAGCAG CAAACAGGAG ATTCGCCGGC AGATGATTGA CGGCCGATCG 
GGAGATATGA CCATTAAAAC TCTAATTAAA TCAATGGACG AGCGCTACAT TGACGACGTC 
ACCCGGATGT ACACCTGGAC GCCCATCAAC GACACAGACT ACAGTCTAGG TTTGGTACTG 
CCTCCGTACA GTGAACATTA CATCCAAGCT GATCTGAGTG ATGTCATGCT CCAGCTGCAG 
TATTTACAGT CTCTGCTGCC CAACTCTTTC GAGTCTGCCG GTCATGTGTT TCTCGCTCCC 
AGAGATTATT GCAGGCGCCT GCACATCTCT GAAAACAACA CACAGTTCCT GGTGGACTTC 
CTGTCTCTGA TGGTGGAGAT CTCACCGGAG TCTGAAGACT GTGATCAGGG TCTCATTCAT 
AACCTGATCT TGGACTCCGG GATCCTCTGG CAGCTCGCCA CACGCGTTTG GCAGAATAAA 
GACCTGGGCA CGTATGGCTT TCTGGCTCTG TTTGCGTCCA CCAATGGAGG CGTCACACGT 
GTTTACCCCA ACATGGCGGC TGAATCATGG GATGAAGATC CTGAACCACT AAACTCAAAC 
TTCTACCGGC GCAGTCTGGA TAACAAAGGC TACATCTTCA GAGCGCCGAC ACGAACCTCG 
ATGGATGATC TGCTGATCTC AGAGAATGGC ACTGTGGGGA TTCTGGTTAC CACCGCCGTA 
GAGGTTGCAT TTGGGAGCAA AACCATGAAG CCTGCAGTGG TCGGCGTTAA GCTGGATTTG 
GAGGCTTGGG TCGACAAGTT CAAGATCTTG GCCAGCAACG TGTCCGACAG CAGACAGGCC 
GCACATAAGT GCGGCCCGTC CAGATCCTGT GAGATGGACT GTGAAGTGAA CAGCGATGAT 
TTGCTGTGCT ATCTGATCGA TGATGGCGGT TTCCTGGTGA TGTCCAATCA GAGAGATCAC 
TGGAAGAAGG TTGGGCTGTT TTTCGGTGAG GTCGACCCGT ACCTGATGTT CGCCCTCTAC 
AACAACTCAA TCTACTCCCG TCATCAGACC TTCCAGTATC AGTCGGCCTG CGAACCCACA 
GCCAGCAGCC ACACAGGAGC CGCACACAGA GGATTCTACG TGCCCTCCAT AGCGGACTTT 
CTCAGCCTAG CCTGGTGGAC GTCTGCAGCC GCGTGGTCTA TTTTGCAGCA GTTCTTGTAT 
GGAGTCATGT ACAGCAGCTG GTTCACCACA GCAGACGTCT GGGCTGAGAG TGCAGACAGT 
AAAGGGAGCA GCAGCTGTGT GACGGTTTAC AGCCAGTTCT ACTTCACCAA CACCACCAAC 
TCCTTCAACG TCCTGCAGGA CTGCGGCAAC TGCTCCAGGT TGTTCCATGC CAAGCGTATA 
GAAAACACAA ACCTTTTGTT TGTGGTGGCT GAGACACTGC CCTGCAGCTC CTGCGAGATC 
GAGAAACTCC TGCCTGTCAG AACCAAGTCT CAAGAAGAAA ACCCATGTGA TGTCTTAAAC 
TCGGCCCGCT ATAGGAAAGG ACCTGACAAA TGCTTCGACT ACAACTCAGA GGAGAACACA 
TCACAGTGTG GCAGCGCTCA CTCTTTACAC TGTCCGCTGC CAGCTCTGCT CTTGGTTCAG 
TTATTTTTGA TTTACCTCAT TCTACGATCG TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009294385.1
CDS	190..3462
Translation	MARWAHACLLLLLMVILGGSWTAVNALTFPQHYTIMHWARRIEQEIDRVLQHISGAQQLKGIYLEKKKHFSLVRNNPRDIVEKVATDIERLLAKKRKALERLASEAERLQKEHRWQDGIKEENIEYYNSKAEMDYDGEDIDSQMSLKLDFVYDPSFKNQVNYSHTAVQIPTDIYKGAPVILNELNWTQALERVFIENSRDDPSLLWQAFGSATGVTRYYPAAPWRAPDKIDLYDVRRRPWYIQGASSPKDMVILVDVSGSVSGLTLKLIKASVTEMLDTLSDDDYVNVARFNEKAEAVVPCFDHLVQANVRNKKIFKEAVQQMQAKGTTDYKSGFHFAFNQLLNKTNVPRANCNKIIMLFTDGGEDRAQDIFEQYNWPNKTVRVFTFSVGQHNYDVTPLQWIACSNKGYYFEIRSICAIRINTQEYLDVLGRPMVLAGRIAKQVQWTNVYQDALGLGLVVTGTMPVFNLTVNGDSQNQLILGVMGVDVHLDELKRLTPQYKLGANGYIFAIDPNGYVLMHPNLQPREEDLPEQVTLDFLDAEVEDSSKQEIRRQMIDGRSGDMTIKTLIKSMDERYIDDVTRMYTWTPINDTDYSLGLVLPPYSEHYIQADLSDVMLQLQYLQSLLPNSFESAGHVFLAPRDYCRRLHISENNTQFLVDFLSLMVEISPESEDCDQGLIHNLILDSGILWQLATRVWQNKDLGTYGFLALFASTNGGVTRVYPNMAAESWDEDPEPLNSNFYRRSLDNKGYIFRAPTRTSMDDLLISENGTVGILVTTAVEVAFGSKTMKPAVVGVKLDLEAWVDKFKILASNVSDSRQAAHKCGPSRSCEMDCEVNSDDLLCYLIDDGGFLVMSNQRDHWKKVGLFFGEVDPYLMFALYNNSIYSRHQTFQYQSACEPTASSHTGAAHRGFYVPSIADFLSLAWWTSAAAWSILQQFLYGVMYSSWFTTADVWAESADSKGSSSCVTVYSQFYFTNTTNSFNVLQDCGNCSRLFHAKRIENTNLLFVVAETLPCSSCEIEKLLPVRTKSQEENPCDVLNSARYRKGPDKCFDYNSEENTSQCGSAHSLHCPLPALLLVQLFLIYLILRS

Target ORF information:

RefSeq Version	XM_009296110.1
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio calcium channel, voltage-dependent, alpha 2/delta subunit 2b (cacna2d2b), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009296110.1

ORF Insert Sequence:

ATGGCGAGAT GGGCTCACGC GTGTCTGCTG CTGCTGCTGA TGGTGATTCT CGGCGGATCC 
TGGACCGCAG TGAACGCGCT CACATTTCCT CAGCATTACA CGATTATGCA CTGGGCTCGA 
CGCATTGAAC AGGAGATCGA CAGAGTTTTG CAGCATATTA GTGGCGCTCA GCAACTGAAG 
GGGATCTACT TGGAAAAGAA AAAGCACTTC AGTCTGGTCA GAAACAACCC TCGAGACATT 
GTAGAGAAAG TAGCGACTGA CATCGAGAGA CTGCTTGCCA AGAAACGCAA AGCGCTGGAG 
AGACTGGCGA GTGAAGCGGA GCGTCTTCAG AAAGAGCATC GCTGGCAGGA TGGAATCAAG 
GAGGAAAATA TTGAGTATTA TAACTCTAAA GCAGAGATGG ATTATGACGG TGAGGACATT 
GACTCTCAGA TGTCCTTGAA ACTGGATTTT GTGTACGACC CAAGCTTCAA AAACCAAGTG 
AATTACTCGC ACACTGCCGT CCAGATCCCC ACAGACATCT ACAAAGGCGC TCCAGTGATT 
CTGAATGAGC TGAACTGGAC GCAGGCGCTA GAGCGAGTCT TCATTGAGAA CAGCAGAGAT 
GATCCCTCAT TGCTTTGGCA AGCATTTGGT AGTGCCACCG GTGTCACCAG ATATTACCCA 
GCTGCTCCCT GGAGAGCGCC AGATAAGATC GACCTTTATG ATGTACGACG AAGACCCTGG 
TACATTCAAG GAGCGTCGTC ACCCAAAGAC ATGGTCATTC TTGTAGACGT GAGCGGCAGC 
GTCAGCGGGC TCACATTAAA ACTGATCAAA GCTTCGGTCA CAGAAATGCT CGACACACTC 
TCCGATGATG ATTATGTCAA CGTGGCCAGG TTTAATGAGA AGGCAGAGGC AGTGGTGCCC 
TGTTTCGATC ACCTGGTTCA GGCCAATGTG CGCAATAAGA AGATCTTCAA AGAGGCTGTG 
CAGCAGATGC AGGCCAAAGG GACCACCGAT TACAAATCCG GCTTCCATTT CGCCTTCAAC 
CAGCTCCTGA ACAAGACAAA CGTTCCTCGA GCCAACTGTA ATAAAATCAT CATGTTGTTC 
ACTGACGGCG GAGAGGACCG AGCGCAGGAC ATCTTCGAGC AGTACAACTG GCCTAATAAG 
ACGGTCCGCG TGTTCACGTT CTCGGTGGGG CAGCACAATT ACGATGTCAC TCCGCTGCAG 
TGGATTGCAT GCTCTAATAA GGGATACTAT TTTGAGATCC GCTCCATCTG TGCTATAAGG 
ATTAACACCC AGGAATATCT GGATGTATTG GGTCGCCCGA TGGTTCTGGC AGGCAGAATC 
GCCAAACAAG TACAGTGGAC AAACGTCTAT CAGGACGCCC TGGGTCTGGG TCTGGTGGTG 
ACAGGGACAA TGCCGGTGTT CAACCTTACT GTGAACGGGG ACTCTCAGAA TCAGCTGATT 
TTAGGTGTGA TGGGGGTCGA CGTTCATCTG GACGAGCTGA AGAGACTCAC ACCACAGTAC 
AAGCTCGGAG CCAATGGATA CATCTTCGCC ATTGACCCGA ACGGATACGT GCTGATGCAT 
CCAAACCTGC AGCCTCGAGA AGAGGATTTA CCGGAGCAGG TGACTCTGGA CTTTCTGGAT 
GCAGAAGTGG AAGACAGCAG CAAACAGGAG ATTCGCCGGC AGATGATTGA CGGCCGATCG 
GGAGATATGA CCATTAAAAC TCTAATTAAA TCAATGGACG AGCGCTACAT TGACGACGTC 
ACCCGGATGT ACACCTGGAC GCCCATCAAC GACACAGACT ACAGTCTAGG TTTGGTACTG 
CCTCCGTACA GTGAACATTA CATCCAAGCT GATCTGAGTG ATGTCATGCT CCAGCTGCAG 
TATTTACAGT CTCTGCTGCC CAACTCTTTC GAGTCTGCCG GTCATGTGTT TCTCGCTCCC 
AGAGATTATT GCAGGCGCCT GCACATCTCT GAAAACAACA CACAGTTCCT GGTGGACTTC 
CTGTCTCTGA TGGTGGAGAT CTCACCGGAG TCTGAAGACT GTGATCAGGG TCTCATTCAT 
AACCTGATCT TGGACTCCGG GATCCTCTGG CAGCTCGCCA CACGCGTTTG GCAGAATAAA 
GACCTGGGCA CGTATGGCTT TCTGGCTCTG TTTGCGTCCA CCAATGGAGG CGTCACACGT 
GTTTACCCCA ACATGGCGGC TGAATCATGG GATGAAGATC CTGAACCACT AAACTCAAAC 
TTCTACCGGC GCAGTCTGGA TAACAAAGGC TACATCTTCA GAGCGCCGAC ACGAACCTCG 
ATGGATGATC TGCTGATCTC AGAGAATGGC ACTGTGGGGA TTCTGGTTAC CACCGCCGTA 
GAGGTTGCAT TTGGGAGCAA AACCATGAAG CCTGCAGTGG TCGGCGTTAA GCTGGATTTG 
GAGGCTTGGG TCGACAAGTT CAAGATCTTG GCCAGCAACG TGTCCGACAG CAGACAGGCC 
GCACATAAGT GCGGCCCGTC CAGATCCTGT GAGATGGACT GTGAAGTGAA CAGCGATGAT 
TTGCTGTGCT ATCTGATCGA TGATGGCGGT TTCCTGGTGA TGTCCAATCA GAGAGATCAC 
TGGAAGAAGG TTGGGCTGTT TTTCGGTGAG GTCGACCCGT ACCTGATGTT CGCCCTCTAC 
AACAACTCAA TCTACTCCCG TCATCAGACC TTCCAGTATC AGTCGGCCTG CGAACCCACA 
GCCAGCAGCC ACACAGGAGC CGCACACAGA GGATTCTACG TGCCCTCCAT AGCGGACTTT 
CTCAGCCTAG CCTGGTGGAC GTCTGCAGCC GCGTGGTCTA TTTTGCAGCA GTTCTTGTAT 
GGAGTCATGT ACAGCAGCTG GTTCACCACA GCAGACGTCT GGGCTGAGAG TGCAGACAGT 
AAAGGGAGCA GCAGCTGTGT GACGGTTTAC AGCCAGTTCT ACTTCACCAA CACCACCAAC 
TCCTTCAACG TCCTGCAGGA CTGCGGCAAC TGCTCCAGGT TGTTCCATGC CAAGCGTATA 
GAAAACACAA ACCTTTTGTT TGTGGTGGCT GAGACACTGC CCTGCAGCTC CTGCGAGATC 
GAGAAACTCC TGCCTGTCAG AACCAAGTCT CAAGAAGAAA ACCCATGTGA TGTCTTAAAC 
TCGGCCCGCT ATAGGAAAGG ACCTGACAAA TGCTTCGACT ACAACTCAGA GGAGAACACA 
TCACAGTGTG GCAGCGCTCA CTCTTTACAC TGTCCGCTGC CAGCTCTGCT CTTGGTTCAG 
TTATTTTTGA TTTACCTCAT TCTACGATCG TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

cacna2d2b ( XM_009296110.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009294385.1 Danio rerio calcium channel, voltage-dependent, alpha 2/delta subunit 2b (cacna2d2b), transcript variant X1, mRNA.

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CloneID	ODa26699
Clone ID Related Accession (Same CDS sequence)	XM_009296110.1 , XM_009296110.2
Accession Version	XM_009296110.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3273bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	calcium channel, voltage-dependent, alpha 2/delta subunit 2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007133.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009296110.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGAGAT GGGCTCACGC GTGTCTGCTG CTGCTGCTGA TGGTGATTCT CGGCGGATCC 
TGGACCGCAG TGAACGCGCT CACATTTCCT CAGCATTACA CGATTATGCA CTGGGCTCGA 
CGCATTGAAC AGGAGATCGA CAGAGTTTTG CAGCATATTA GTGGCGCTCA GCAACTGAAG 
GGGATCTACT TGGAAAAGAA AAAGCACTTC AGTCTGGTCA GAAACAACCC TCGAGACATT 
GTAGAGAAAG TAGCGACTGA CATCGAGAGA CTGCTTGCCA AGAAACGCAA AGCGCTGGAG 
AGACTGGCGA GTGAAGCGGA GCGTCTTCAG AAAGAGCATC GCTGGCAGGA TGGAATCAAG 
GAGGAAAATA TTGAGTATTA TAACTCTAAA GCAGAGATGG ATTATGACGG TGAGGACATT 
GACTCTCAGA TGTCCTTGAA ACTGGATTTT GTGTACGACC CAAGCTTCAA AAACCAAGTG 
AATTACTCGC ACACTGCCGT CCAGATCCCC ACAGACATCT ACAAAGGCGC TCCAGTGATT 
CTGAATGAGC TGAACTGGAC GCAGGCGCTA GAGCGAGTCT TCATTGAGAA CAGCAGAGAT 
GATCCCTCAT TGCTTTGGCA AGCATTTGGT AGTGCCACCG GTGTCACCAG ATATTACCCA 
GCTGCTCCCT GGAGAGCGCC AGATAAGATC GACCTTTATG ATGTACGACG AAGACCCTGG 
TACATTCAAG GAGCGTCGTC ACCCAAAGAC ATGGTCATTC TTGTAGACGT GAGCGGCAGC 
GTCAGCGGGC TCACATTAAA ACTGATCAAA GCTTCGGTCA CAGAAATGCT CGACACACTC 
TCCGATGATG ATTATGTCAA CGTGGCCAGG TTTAATGAGA AGGCAGAGGC AGTGGTGCCC 
TGTTTCGATC ACCTGGTTCA GGCCAATGTG CGCAATAAGA AGATCTTCAA AGAGGCTGTG 
CAGCAGATGC AGGCCAAAGG GACCACCGAT TACAAATCCG GCTTCCATTT CGCCTTCAAC 
CAGCTCCTGA ACAAGACAAA CGTTCCTCGA GCCAACTGTA ATAAAATCAT CATGTTGTTC 
ACTGACGGCG GAGAGGACCG AGCGCAGGAC ATCTTCGAGC AGTACAACTG GCCTAATAAG 
ACGGTCCGCG TGTTCACGTT CTCGGTGGGG CAGCACAATT ACGATGTCAC TCCGCTGCAG 
TGGATTGCAT GCTCTAATAA GGGATACTAT TTTGAGATCC GCTCCATCTG TGCTATAAGG 
ATTAACACCC AGGAATATCT GGATGTATTG GGTCGCCCGA TGGTTCTGGC AGGCAGAATC 
GCCAAACAAG TACAGTGGAC AAACGTCTAT CAGGACGCCC TGGGTCTGGG TCTGGTGGTG 
ACAGGGACAA TGCCGGTGTT CAACCTTACT GTGAACGGGG ACTCTCAGAA TCAGCTGATT 
TTAGGTGTGA TGGGGGTCGA CGTTCATCTG GACGAGCTGA AGAGACTCAC ACCACAGTAC 
AAGCTCGGAG CCAATGGATA CATCTTCGCC ATTGACCCGA ACGGATACGT GCTGATGCAT 
CCAAACCTGC AGCCTCGAGA AGAGGATTTA CCGGAGCAGG TGACTCTGGA CTTTCTGGAT 
GCAGAAGTGG AAGACAGCAG CAAACAGGAG ATTCGCCGGC AGATGATTGA CGGCCGATCG 
GGAGATATGA CCATTAAAAC TCTAATTAAA TCAATGGACG AGCGCTACAT TGACGACGTC 
ACCCGGATGT ACACCTGGAC GCCCATCAAC GACACAGACT ACAGTCTAGG TTTGGTACTG 
CCTCCGTACA GTGAACATTA CATCCAAGCT GATCTGAGTG ATGTCATGCT CCAGCTGCAG 
TATTTACAGT CTCTGCTGCC CAACTCTTTC GAGTCTGCCG GTCATGTGTT TCTCGCTCCC 
AGAGATTATT GCAGGCGCCT GCACATCTCT GAAAACAACA CACAGTTCCT GGTGGACTTC 
CTGTCTCTGA TGGTGGAGAT CTCACCGGAG TCTGAAGACT GTGATCAGGG TCTCATTCAT 
AACCTGATCT TGGACTCCGG GATCCTCTGG CAGCTCGCCA CACGCGTTTG GCAGAATAAA 
GACCTGGGCA CGTATGGCTT TCTGGCTCTG TTTGCGTCCA CCAATGGAGG CGTCACACGT 
GTTTACCCCA ACATGGCGGC TGAATCATGG GATGAAGATC CTGAACCACT AAACTCAAAC 
TTCTACCGGC GCAGTCTGGA TAACAAAGGC TACATCTTCA GAGCGCCGAC ACGAACCTCG 
ATGGATGATC TGCTGATCTC AGAGAATGGC ACTGTGGGGA TTCTGGTTAC CACCGCCGTA 
GAGGTTGCAT TTGGGAGCAA AACCATGAAG CCTGCAGTGG TCGGCGTTAA GCTGGATTTG 
GAGGCTTGGG TCGACAAGTT CAAGATCTTG GCCAGCAACG TGTCCGACAG CAGACAGGCC 
GCACATAAGT GCGGCCCGTC CAGATCCTGT GAGATGGACT GTGAAGTGAA CAGCGATGAT 
TTGCTGTGCT ATCTGATCGA TGATGGCGGT TTCCTGGTGA TGTCCAATCA GAGAGATCAC 
TGGAAGAAGG TTGGGCTGTT TTTCGGTGAG GTCGACCCGT ACCTGATGTT CGCCCTCTAC 
AACAACTCAA TCTACTCCCG TCATCAGACC TTCCAGTATC AGTCGGCCTG CGAACCCACA 
GCCAGCAGCC ACACAGGAGC CGCACACAGA GGATTCTACG TGCCCTCCAT AGCGGACTTT 
CTCAGCCTAG CCTGGTGGAC GTCTGCAGCC GCGTGGTCTA TTTTGCAGCA GTTCTTGTAT 
GGAGTCATGT ACAGCAGCTG GTTCACCACA GCAGACGTCT GGGCTGAGAG TGCAGACAGT 
AAAGGGAGCA GCAGCTGTGT GACGGTTTAC AGCCAGTTCT ACTTCACCAA CACCACCAAC 
TCCTTCAACG TCCTGCAGGA CTGCGGCAAC TGCTCCAGGT TGTTCCATGC CAAGCGTATA 
GAAAACACAA ACCTTTTGTT TGTGGTGGCT GAGACACTGC CCTGCAGCTC CTGCGAGATC 
GAGAAACTCC TGCCTGTCAG AACCAAGTCT CAAGAAGAAA ACCCATGTGA TGTCTTAAAC 
TCGGCCCGCT ATAGGAAAGG ACCTGACAAA TGCTTCGACT ACAACTCAGA GGAGAACACA 
TCACAGTGTG GCAGCGCTCA CTCTTTACAC TGTCCGCTGC CAGCTCTGCT CTTGGTTCAG 
TTATTTTTGA TTTACCTCAT TCTACGATCG TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009294385.1
CDS	207..3479
Translation	MARWAHACLLLLLMVILGGSWTAVNALTFPQHYTIMHWARRIEQEIDRVLQHISGAQQLKGIYLEKKKHFSLVRNNPRDIVEKVATDIERLLAKKRKALERLASEAERLQKEHRWQDGIKEENIEYYNSKAEMDYDGEDIDSQMSLKLDFVYDPSFKNQVNYSHTAVQIPTDIYKGAPVILNELNWTQALERVFIENSRDDPSLLWQAFGSATGVTRYYPAAPWRAPDKIDLYDVRRRPWYIQGASSPKDMVILVDVSGSVSGLTLKLIKASVTEMLDTLSDDDYVNVARFNEKAEAVVPCFDHLVQANVRNKKIFKEAVQQMQAKGTTDYKSGFHFAFNQLLNKTNVPRANCNKIIMLFTDGGEDRAQDIFEQYNWPNKTVRVFTFSVGQHNYDVTPLQWIACSNKGYYFEIRSICAIRINTQEYLDVLGRPMVLAGRIAKQVQWTNVYQDALGLGLVVTGTMPVFNLTVNGDSQNQLILGVMGVDVHLDELKRLTPQYKLGANGYIFAIDPNGYVLMHPNLQPREEDLPEQVTLDFLDAEVEDSSKQEIRRQMIDGRSGDMTIKTLIKSMDERYIDDVTRMYTWTPINDTDYSLGLVLPPYSEHYIQADLSDVMLQLQYLQSLLPNSFESAGHVFLAPRDYCRRLHISENNTQFLVDFLSLMVEISPESEDCDQGLIHNLILDSGILWQLATRVWQNKDLGTYGFLALFASTNGGVTRVYPNMAAESWDEDPEPLNSNFYRRSLDNKGYIFRAPTRTSMDDLLISENGTVGILVTTAVEVAFGSKTMKPAVVGVKLDLEAWVDKFKILASNVSDSRQAAHKCGPSRSCEMDCEVNSDDLLCYLIDDGGFLVMSNQRDHWKKVGLFFGEVDPYLMFALYNNSIYSRHQTFQYQSACEPTASSHTGAAHRGFYVPSIADFLSLAWWTSAAAWSILQQFLYGVMYSSWFTTADVWAESADSKGSSSCVTVYSQFYFTNTTNSFNVLQDCGNCSRLFHAKRIENTNLLFVVAETLPCSSCEIEKLLPVRTKSQEENPCDVLNSARYRKGPDKCFDYNSEENTSQCGSAHSLHCPLPALLLVQLFLIYLILRS

Target ORF information:

RefSeq Version	XM_009296110.2
Organism	Danio rerio(zebrafish)
Definition	Danio rerio calcium channel, voltage-dependent, alpha 2/delta subunit 2b (cacna2d2b), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009296110.2

ORF Insert Sequence:

ATGGCGAGAT GGGCTCACGC GTGTCTGCTG CTGCTGCTGA TGGTGATTCT CGGCGGATCC 
TGGACCGCAG TGAACGCGCT CACATTTCCT CAGCATTACA CGATTATGCA CTGGGCTCGA 
CGCATTGAAC AGGAGATCGA CAGAGTTTTG CAGCATATTA GTGGCGCTCA GCAACTGAAG 
GGGATCTACT TGGAAAAGAA AAAGCACTTC AGTCTGGTCA GAAACAACCC TCGAGACATT 
GTAGAGAAAG TAGCGACTGA CATCGAGAGA CTGCTTGCCA AGAAACGCAA AGCGCTGGAG 
AGACTGGCGA GTGAAGCGGA GCGTCTTCAG AAAGAGCATC GCTGGCAGGA TGGAATCAAG 
GAGGAAAATA TTGAGTATTA TAACTCTAAA GCAGAGATGG ATTATGACGG TGAGGACATT 
GACTCTCAGA TGTCCTTGAA ACTGGATTTT GTGTACGACC CAAGCTTCAA AAACCAAGTG 
AATTACTCGC ACACTGCCGT CCAGATCCCC ACAGACATCT ACAAAGGCGC TCCAGTGATT 
CTGAATGAGC TGAACTGGAC GCAGGCGCTA GAGCGAGTCT TCATTGAGAA CAGCAGAGAT 
GATCCCTCAT TGCTTTGGCA AGCATTTGGT AGTGCCACCG GTGTCACCAG ATATTACCCA 
GCTGCTCCCT GGAGAGCGCC AGATAAGATC GACCTTTATG ATGTACGACG AAGACCCTGG 
TACATTCAAG GAGCGTCGTC ACCCAAAGAC ATGGTCATTC TTGTAGACGT GAGCGGCAGC 
GTCAGCGGGC TCACATTAAA ACTGATCAAA GCTTCGGTCA CAGAAATGCT CGACACACTC 
TCCGATGATG ATTATGTCAA CGTGGCCAGG TTTAATGAGA AGGCAGAGGC AGTGGTGCCC 
TGTTTCGATC ACCTGGTTCA GGCCAATGTG CGCAATAAGA AGATCTTCAA AGAGGCTGTG 
CAGCAGATGC AGGCCAAAGG GACCACCGAT TACAAATCCG GCTTCCATTT CGCCTTCAAC 
CAGCTCCTGA ACAAGACAAA CGTTCCTCGA GCCAACTGTA ATAAAATCAT CATGTTGTTC 
ACTGACGGCG GAGAGGACCG AGCGCAGGAC ATCTTCGAGC AGTACAACTG GCCTAATAAG 
ACGGTCCGCG TGTTCACGTT CTCGGTGGGG CAGCACAATT ACGATGTCAC TCCGCTGCAG 
TGGATTGCAT GCTCTAATAA GGGATACTAT TTTGAGATCC GCTCCATCTG TGCTATAAGG 
ATTAACACCC AGGAATATCT GGATGTATTG GGTCGCCCGA TGGTTCTGGC AGGCAGAATC 
GCCAAACAAG TACAGTGGAC AAACGTCTAT CAGGACGCCC TGGGTCTGGG TCTGGTGGTG 
ACAGGGACAA TGCCGGTGTT CAACCTTACT GTGAACGGGG ACTCTCAGAA TCAGCTGATT 
TTAGGTGTGA TGGGGGTCGA CGTTCATCTG GACGAGCTGA AGAGACTCAC ACCACAGTAC 
AAGCTCGGAG CCAATGGATA CATCTTCGCC ATTGACCCGA ACGGATACGT GCTGATGCAT 
CCAAACCTGC AGCCTCGAGA AGAGGATTTA CCGGAGCAGG TGACTCTGGA CTTTCTGGAT 
GCAGAAGTGG AAGACAGCAG CAAACAGGAG ATTCGCCGGC AGATGATTGA CGGCCGATCG 
GGAGATATGA CCATTAAAAC TCTAATTAAA TCAATGGACG AGCGCTACAT TGACGACGTC 
ACCCGGATGT ACACCTGGAC GCCCATCAAC GACACAGACT ACAGTCTAGG TTTGGTACTG 
CCTCCGTACA GTGAACATTA CATCCAAGCT GATCTGAGTG ATGTCATGCT CCAGCTGCAG 
TATTTACAGT CTCTGCTGCC CAACTCTTTC GAGTCTGCCG GTCATGTGTT TCTCGCTCCC 
AGAGATTATT GCAGGCGCCT GCACATCTCT GAAAACAACA CACAGTTCCT GGTGGACTTC 
CTGTCTCTGA TGGTGGAGAT CTCACCGGAG TCTGAAGACT GTGATCAGGG TCTCATTCAT 
AACCTGATCT TGGACTCCGG GATCCTCTGG CAGCTCGCCA CACGCGTTTG GCAGAATAAA 
GACCTGGGCA CGTATGGCTT TCTGGCTCTG TTTGCGTCCA CCAATGGAGG CGTCACACGT 
GTTTACCCCA ACATGGCGGC TGAATCATGG GATGAAGATC CTGAACCACT AAACTCAAAC 
TTCTACCGGC GCAGTCTGGA TAACAAAGGC TACATCTTCA GAGCGCCGAC ACGAACCTCG 
ATGGATGATC TGCTGATCTC AGAGAATGGC ACTGTGGGGA TTCTGGTTAC CACCGCCGTA 
GAGGTTGCAT TTGGGAGCAA AACCATGAAG CCTGCAGTGG TCGGCGTTAA GCTGGATTTG 
GAGGCTTGGG TCGACAAGTT CAAGATCTTG GCCAGCAACG TGTCCGACAG CAGACAGGCC 
GCACATAAGT GCGGCCCGTC CAGATCCTGT GAGATGGACT GTGAAGTGAA CAGCGATGAT 
TTGCTGTGCT ATCTGATCGA TGATGGCGGT TTCCTGGTGA TGTCCAATCA GAGAGATCAC 
TGGAAGAAGG TTGGGCTGTT TTTCGGTGAG GTCGACCCGT ACCTGATGTT CGCCCTCTAC 
AACAACTCAA TCTACTCCCG TCATCAGACC TTCCAGTATC AGTCGGCCTG CGAACCCACA 
GCCAGCAGCC ACACAGGAGC CGCACACAGA GGATTCTACG TGCCCTCCAT AGCGGACTTT 
CTCAGCCTAG CCTGGTGGAC GTCTGCAGCC GCGTGGTCTA TTTTGCAGCA GTTCTTGTAT 
GGAGTCATGT ACAGCAGCTG GTTCACCACA GCAGACGTCT GGGCTGAGAG TGCAGACAGT 
AAAGGGAGCA GCAGCTGTGT GACGGTTTAC AGCCAGTTCT ACTTCACCAA CACCACCAAC 
TCCTTCAACG TCCTGCAGGA CTGCGGCAAC TGCTCCAGGT TGTTCCATGC CAAGCGTATA 
GAAAACACAA ACCTTTTGTT TGTGGTGGCT GAGACACTGC CCTGCAGCTC CTGCGAGATC 
GAGAAACTCC TGCCTGTCAG AACCAAGTCT CAAGAAGAAA ACCCATGTGA TGTCTTAAAC 
TCGGCCCGCT ATAGGAAAGG ACCTGACAAA TGCTTCGACT ACAACTCAGA GGAGAACACA 
TCACAGTGTG GCAGCGCTCA CTCTTTACAC TGTCCGCTGC CAGCTCTGCT CTTGGTTCAG 
TTATTTTTGA TTTACCTCAT TCTACGATCG TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

cacna2d2b ( XM_009296112.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009294387.1 Danio rerio calcium channel, voltage-dependent, alpha 2/delta subunit 2b (cacna2d2b), transcript variant X3, mRNA.

Price & Availability↑

CloneID	ODa26700
Clone ID Related Accession (Same CDS sequence)	XM_009296112.1 , XM_009296112.2
Accession Version	XM_009296112.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3228bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	calcium channel, voltage-dependent, alpha 2/delta subunit 2 isoform X3
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007133.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGAGAT GGGCTCACGC GTGTCTGCTG CTGCTGCTGA TGGTGATTCT CGGCGGATCC 
TGGACCGCAG TGAACGCGCT CACATTTCCT CAGCATTACA CGATTATGCA CTGGGCTCGA 
CGCATTGAAC AGGAGATCGA CAGAGTTTTG CAGCATATTA GTGGCGCTCA GCAACTGAAG 
GGGATCTACT TGGAAAAGAA AAAGCACTTC AGTCTGGTCA GAAACAACCC TCGAGACATT 
GTAGAGAAAG TAGCGACTGA CATCGAGAGA CTGCTTGCCA AGAAACGCAA AGCGCTGGAG 
AGACTGGCGA GTGAAGCGGA GCGTCTTCAG AAAGAGCATC GCTGGCAGGA TGGAATCAAG 
GACGGTGAGG ACATTGACTC TCAGATGTCC TTGAAACTGG ATTTTGTGTA CGACCCAAGC 
TTCAAAAACC AAGTGAATTA CTCGCACACT GCCGTCCAGA TCCCCACAGA CATCTACAAA 
GGCGCTCCAG TGATTCTGAA TGAGCTGAAC TGGACGCAGG CGCTAGAGCG AGTCTTCATT 
GAGAACAGCA GAGATGATCC CTCATTGCTT TGGCAAGCAT TTGGTAGTGC CACCGGTGTC 
ACCAGATATT ACCCAGCTGC TCCCTGGAGA GCGCCAGATA AGATCGACCT TTATGATGTA 
CGACGAAGAC CCTGGTACAT TCAAGGAGCG TCGTCACCCA AAGACATGGT CATTCTTGTA 
GACGTGAGCG GCAGCGTCAG CGGGCTCACA TTAAAACTGA TCAAAGCTTC GGTCACAGAA 
ATGCTCGACA CACTCTCCGA TGATGATTAT GTCAACGTGG CCAGGTTTAA TGAGAAGGCA 
GAGGCAGTGG TGCCCTGTTT CGATCACCTG GTTCAGGCCA ATGTGCGCAA TAAGAAGATC 
TTCAAAGAGG CTGTGCAGCA GATGCAGGCC AAAGGGACCA CCGATTACAA ATCCGGCTTC 
CATTTCGCCT TCAACCAGCT CCTGAACAAG ACAAACGTTC CTCGAGCCAA CTGTAATAAA 
ATCATCATGT TGTTCACTGA CGGCGGAGAG GACCGAGCGC AGGACATCTT CGAGCAGTAC 
AACTGGCCTA ATAAGACGGT CCGCGTGTTC ACGTTCTCGG TGGGGCAGCA CAATTACGAT 
GTCACTCCGC TGCAGTGGAT TGCATGCTCT AATAAGGGAT ACTATTTTGA GATCCGCTCC 
ATCTGTGCTA TAAGGATTAA CACCCAGGAA TATCTGGATG TATTGGGTCG CCCGATGGTT 
CTGGCAGGCA GAATCGCCAA ACAAGTACAG TGGACAAACG TCTATCAGGA CGCCCTGGGT 
CTGGGTCTGG TGGTGACAGG GACAATGCCG GTGTTCAACC TTACTGTGAA CGGGGACTCT 
CAGAATCAGC TGATTTTAGG TGTGATGGGG GTCGACGTTC ATCTGGACGA GCTGAAGAGA 
CTCACACCAC AGTACAAGCT CGGAGCCAAT GGATACATCT TCGCCATTGA CCCGAACGGA 
TACGTGCTGA TGCATCCAAA CCTGCAGCCT CGAGAAGAGG ATTTACCGGA GCAGGTGACT 
CTGGACTTTC TGGATGCAGA AGTGGAAGAC AGCAGCAAAC AGGAGATTCG CCGGCAGATG 
ATTGACGGCC GATCGGGAGA TATGACCATT AAAACTCTAA TTAAATCAAT GGACGAGCGC 
TACATTGACG ACGTCACCCG GATGTACACC TGGACGCCCA TCAACGACAC AGACTACAGT 
CTAGGTTTGG TACTGCCTCC GTACAGTGAA CATTACATCC AAGCTGATCT GAGTGATGTC 
ATGCTCCAGC TGCAGTATTT ACAGTCTCTG CTGCCCAACT CTTTCGAGTC TGCCGGTCAT 
GTGTTTCTCG CTCCCAGAGA TTATTGCAGG CGCCTGCACA TCTCTGAAAA CAACACACAG 
TTCCTGGTGG ACTTCCTGTC TCTGATGGTG GAGATCTCAC CGGAGTCTGA AGACTGTGAT 
CAGGGTCTCA TTCATAACCT GATCTTGGAC TCCGGGATCC TCTGGCAGCT CGCCACACGC 
GTTTGGCAGA ATAAAGACCT GGGCACGTAT GGCTTTCTGG CTCTGTTTGC GTCCACCAAT 
GGAGGCGTCA CACGTGTTTA CCCCAACATG GCGGCTGAAT CATGGGATGA AGATCCTGAA 
CCACTAAACT CAAACTTCTA CCGGCGCAGT CTGGATAACA AAGGCTACAT CTTCAGAGCG 
CCGACACGAA CCTCGATGGA TGATCTGCTG ATCTCAGAGA ATGGCACTGT GGGGATTCTG 
GTTACCACCG CCGTAGAGGT TGCATTTGGG AGCAAAACCA TGAAGCCTGC AGTGGTCGGC 
GTTAAGCTGG ATTTGGAGGC TTGGGTCGAC AAGTTCAAGA TCTTGGCCAG CAACGTGTCC 
GACAGCAGAC AGGCCGCACA TAAGTGCGGC CCGTCCAGAT CCTGTGAGAT GGACTGTGAA 
GTGAACAGCG ATGATTTGCT GTGCTATCTG ATCGATGATG GCGGTTTCCT GGTGATGTCC 
AATCAGAGAG ATCACTGGAA GAAGGTTGGG CTGTTTTTCG GTGAGGTCGA CCCGTACCTG 
ATGTTCGCCC TCTACAACAA CTCAATCTAC TCCCGTCATC AGACCTTCCA GTATCAGTCG 
GCCTGCGAAC CCACAGCCAG CAGCCACACA GGAGCCGCAC ACAGAGGATT CTACGTGCCC 
TCCATAGCGG ACTTTCTCAG CCTAGCCTGG TGGACGTCTG CAGCCGCGTG GTCTATTTTG 
CAGCAGTTCT TGTATGGAGT CATGTACAGC AGCTGGTTCA CCACAGCAGA CGTCTGGGCT 
GAGAGTGCAG ACAGTAAAGG GAGCAGCAGC TGTGTGACGG TTTACAGCCA GTTCTACTTC 
ACCAACACCA CCAACTCCTT CAACGTCCTG CAGGACTGCG GCAACTGCTC CAGGTTGTTC 
CATGCCAAGC GTATAGAAAA CACAAACCTT TTGTTTGTGG TGGCTGAGAC ACTGCCCTGC 
AGCTCCTGCG AGATCGAGAA ACTCCTGCCT GTCAGAACCA AGTCTCAAGA AGAAAACCCA 
TGTGATGTCT TAAACTCGGC CCGCTATAGG AAAGGACCTG ACAAATGCTT CGACTACAAC 
TCAGAGGAGA ACACATCACA GTGTGGCAGC GCTCACTCTT TACACTGTCC GCTGCCAGCT 
CTGCTCTTGG TTCAGTTATT TTTGATTTAC CTCATTCTAC GATCGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009294387.1
CDS	190..3417
Translation	MARWAHACLLLLLMVILGGSWTAVNALTFPQHYTIMHWARRIEQEIDRVLQHISGAQQLKGIYLEKKKHFSLVRNNPRDIVEKVATDIERLLAKKRKALERLASEAERLQKEHRWQDGIKDGEDIDSQMSLKLDFVYDPSFKNQVNYSHTAVQIPTDIYKGAPVILNELNWTQALERVFIENSRDDPSLLWQAFGSATGVTRYYPAAPWRAPDKIDLYDVRRRPWYIQGASSPKDMVILVDVSGSVSGLTLKLIKASVTEMLDTLSDDDYVNVARFNEKAEAVVPCFDHLVQANVRNKKIFKEAVQQMQAKGTTDYKSGFHFAFNQLLNKTNVPRANCNKIIMLFTDGGEDRAQDIFEQYNWPNKTVRVFTFSVGQHNYDVTPLQWIACSNKGYYFEIRSICAIRINTQEYLDVLGRPMVLAGRIAKQVQWTNVYQDALGLGLVVTGTMPVFNLTVNGDSQNQLILGVMGVDVHLDELKRLTPQYKLGANGYIFAIDPNGYVLMHPNLQPREEDLPEQVTLDFLDAEVEDSSKQEIRRQMIDGRSGDMTIKTLIKSMDERYIDDVTRMYTWTPINDTDYSLGLVLPPYSEHYIQADLSDVMLQLQYLQSLLPNSFESAGHVFLAPRDYCRRLHISENNTQFLVDFLSLMVEISPESEDCDQGLIHNLILDSGILWQLATRVWQNKDLGTYGFLALFASTNGGVTRVYPNMAAESWDEDPEPLNSNFYRRSLDNKGYIFRAPTRTSMDDLLISENGTVGILVTTAVEVAFGSKTMKPAVVGVKLDLEAWVDKFKILASNVSDSRQAAHKCGPSRSCEMDCEVNSDDLLCYLIDDGGFLVMSNQRDHWKKVGLFFGEVDPYLMFALYNNSIYSRHQTFQYQSACEPTASSHTGAAHRGFYVPSIADFLSLAWWTSAAAWSILQQFLYGVMYSSWFTTADVWAESADSKGSSSCVTVYSQFYFTNTTNSFNVLQDCGNCSRLFHAKRIENTNLLFVVAETLPCSSCEIEKLLPVRTKSQEENPCDVLNSARYRKGPDKCFDYNSEENTSQCGSAHSLHCPLPALLLVQLFLIYLILRS

Target ORF information:

RefSeq Version	XM_009296112.1
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio calcium channel, voltage-dependent, alpha 2/delta subunit 2b (cacna2d2b), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009296112.1

ORF Insert Sequence:

ATGGCGAGAT GGGCTCACGC GTGTCTGCTG CTGCTGCTGA TGGTGATTCT CGGCGGATCC 
TGGACCGCAG TGAACGCGCT CACATTTCCT CAGCATTACA CGATTATGCA CTGGGCTCGA 
CGCATTGAAC AGGAGATCGA CAGAGTTTTG CAGCATATTA GTGGCGCTCA GCAACTGAAG 
GGGATCTACT TGGAAAAGAA AAAGCACTTC AGTCTGGTCA GAAACAACCC TCGAGACATT 
GTAGAGAAAG TAGCGACTGA CATCGAGAGA CTGCTTGCCA AGAAACGCAA AGCGCTGGAG 
AGACTGGCGA GTGAAGCGGA GCGTCTTCAG AAAGAGCATC GCTGGCAGGA TGGAATCAAG 
GACGGTGAGG ACATTGACTC TCAGATGTCC TTGAAACTGG ATTTTGTGTA CGACCCAAGC 
TTCAAAAACC AAGTGAATTA CTCGCACACT GCCGTCCAGA TCCCCACAGA CATCTACAAA 
GGCGCTCCAG TGATTCTGAA TGAGCTGAAC TGGACGCAGG CGCTAGAGCG AGTCTTCATT 
GAGAACAGCA GAGATGATCC CTCATTGCTT TGGCAAGCAT TTGGTAGTGC CACCGGTGTC 
ACCAGATATT ACCCAGCTGC TCCCTGGAGA GCGCCAGATA AGATCGACCT TTATGATGTA 
CGACGAAGAC CCTGGTACAT TCAAGGAGCG TCGTCACCCA AAGACATGGT CATTCTTGTA 
GACGTGAGCG GCAGCGTCAG CGGGCTCACA TTAAAACTGA TCAAAGCTTC GGTCACAGAA 
ATGCTCGACA CACTCTCCGA TGATGATTAT GTCAACGTGG CCAGGTTTAA TGAGAAGGCA 
GAGGCAGTGG TGCCCTGTTT CGATCACCTG GTTCAGGCCA ATGTGCGCAA TAAGAAGATC 
TTCAAAGAGG CTGTGCAGCA GATGCAGGCC AAAGGGACCA CCGATTACAA ATCCGGCTTC 
CATTTCGCCT TCAACCAGCT CCTGAACAAG ACAAACGTTC CTCGAGCCAA CTGTAATAAA 
ATCATCATGT TGTTCACTGA CGGCGGAGAG GACCGAGCGC AGGACATCTT CGAGCAGTAC 
AACTGGCCTA ATAAGACGGT CCGCGTGTTC ACGTTCTCGG TGGGGCAGCA CAATTACGAT 
GTCACTCCGC TGCAGTGGAT TGCATGCTCT AATAAGGGAT ACTATTTTGA GATCCGCTCC 
ATCTGTGCTA TAAGGATTAA CACCCAGGAA TATCTGGATG TATTGGGTCG CCCGATGGTT 
CTGGCAGGCA GAATCGCCAA ACAAGTACAG TGGACAAACG TCTATCAGGA CGCCCTGGGT 
CTGGGTCTGG TGGTGACAGG GACAATGCCG GTGTTCAACC TTACTGTGAA CGGGGACTCT 
CAGAATCAGC TGATTTTAGG TGTGATGGGG GTCGACGTTC ATCTGGACGA GCTGAAGAGA 
CTCACACCAC AGTACAAGCT CGGAGCCAAT GGATACATCT TCGCCATTGA CCCGAACGGA 
TACGTGCTGA TGCATCCAAA CCTGCAGCCT CGAGAAGAGG ATTTACCGGA GCAGGTGACT 
CTGGACTTTC TGGATGCAGA AGTGGAAGAC AGCAGCAAAC AGGAGATTCG CCGGCAGATG 
ATTGACGGCC GATCGGGAGA TATGACCATT AAAACTCTAA TTAAATCAAT GGACGAGCGC 
TACATTGACG ACGTCACCCG GATGTACACC TGGACGCCCA TCAACGACAC AGACTACAGT 
CTAGGTTTGG TACTGCCTCC GTACAGTGAA CATTACATCC AAGCTGATCT GAGTGATGTC 
ATGCTCCAGC TGCAGTATTT ACAGTCTCTG CTGCCCAACT CTTTCGAGTC TGCCGGTCAT 
GTGTTTCTCG CTCCCAGAGA TTATTGCAGG CGCCTGCACA TCTCTGAAAA CAACACACAG 
TTCCTGGTGG ACTTCCTGTC TCTGATGGTG GAGATCTCAC CGGAGTCTGA AGACTGTGAT 
CAGGGTCTCA TTCATAACCT GATCTTGGAC TCCGGGATCC TCTGGCAGCT CGCCACACGC 
GTTTGGCAGA ATAAAGACCT GGGCACGTAT GGCTTTCTGG CTCTGTTTGC GTCCACCAAT 
GGAGGCGTCA CACGTGTTTA CCCCAACATG GCGGCTGAAT CATGGGATGA AGATCCTGAA 
CCACTAAACT CAAACTTCTA CCGGCGCAGT CTGGATAACA AAGGCTACAT CTTCAGAGCG 
CCGACACGAA CCTCGATGGA TGATCTGCTG ATCTCAGAGA ATGGCACTGT GGGGATTCTG 
GTTACCACCG CCGTAGAGGT TGCATTTGGG AGCAAAACCA TGAAGCCTGC AGTGGTCGGC 
GTTAAGCTGG ATTTGGAGGC TTGGGTCGAC AAGTTCAAGA TCTTGGCCAG CAACGTGTCC 
GACAGCAGAC AGGCCGCACA TAAGTGCGGC CCGTCCAGAT CCTGTGAGAT GGACTGTGAA 
GTGAACAGCG ATGATTTGCT GTGCTATCTG ATCGATGATG GCGGTTTCCT GGTGATGTCC 
AATCAGAGAG ATCACTGGAA GAAGGTTGGG CTGTTTTTCG GTGAGGTCGA CCCGTACCTG 
ATGTTCGCCC TCTACAACAA CTCAATCTAC TCCCGTCATC AGACCTTCCA GTATCAGTCG 
GCCTGCGAAC CCACAGCCAG CAGCCACACA GGAGCCGCAC ACAGAGGATT CTACGTGCCC 
TCCATAGCGG ACTTTCTCAG CCTAGCCTGG TGGACGTCTG CAGCCGCGTG GTCTATTTTG 
CAGCAGTTCT TGTATGGAGT CATGTACAGC AGCTGGTTCA CCACAGCAGA CGTCTGGGCT 
GAGAGTGCAG ACAGTAAAGG GAGCAGCAGC TGTGTGACGG TTTACAGCCA GTTCTACTTC 
ACCAACACCA CCAACTCCTT CAACGTCCTG CAGGACTGCG GCAACTGCTC CAGGTTGTTC 
CATGCCAAGC GTATAGAAAA CACAAACCTT TTGTTTGTGG TGGCTGAGAC ACTGCCCTGC 
AGCTCCTGCG AGATCGAGAA ACTCCTGCCT GTCAGAACCA AGTCTCAAGA AGAAAACCCA 
TGTGATGTCT TAAACTCGGC CCGCTATAGG AAAGGACCTG ACAAATGCTT CGACTACAAC 
TCAGAGGAGA ACACATCACA GTGTGGCAGC GCTCACTCTT TACACTGTCC GCTGCCAGCT 
CTGCTCTTGG TTCAGTTATT TTTGATTTAC CTCATTCTAC GATCGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

cacna2d2b ( XM_009296112.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009294387.1 Danio rerio calcium channel, voltage-dependent, alpha 2/delta subunit 2b (cacna2d2b), transcript variant X3, mRNA.

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CloneID	ODa26700
Clone ID Related Accession (Same CDS sequence)	XM_009296112.1 , XM_009296112.2
Accession Version	XM_009296112.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3228bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	calcium channel, voltage-dependent, alpha 2/delta subunit 2 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007133.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009296112.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGAGAT GGGCTCACGC GTGTCTGCTG CTGCTGCTGA TGGTGATTCT CGGCGGATCC 
TGGACCGCAG TGAACGCGCT CACATTTCCT CAGCATTACA CGATTATGCA CTGGGCTCGA 
CGCATTGAAC AGGAGATCGA CAGAGTTTTG CAGCATATTA GTGGCGCTCA GCAACTGAAG 
GGGATCTACT TGGAAAAGAA AAAGCACTTC AGTCTGGTCA GAAACAACCC TCGAGACATT 
GTAGAGAAAG TAGCGACTGA CATCGAGAGA CTGCTTGCCA AGAAACGCAA AGCGCTGGAG 
AGACTGGCGA GTGAAGCGGA GCGTCTTCAG AAAGAGCATC GCTGGCAGGA TGGAATCAAG 
GACGGTGAGG ACATTGACTC TCAGATGTCC TTGAAACTGG ATTTTGTGTA CGACCCAAGC 
TTCAAAAACC AAGTGAATTA CTCGCACACT GCCGTCCAGA TCCCCACAGA CATCTACAAA 
GGCGCTCCAG TGATTCTGAA TGAGCTGAAC TGGACGCAGG CGCTAGAGCG AGTCTTCATT 
GAGAACAGCA GAGATGATCC CTCATTGCTT TGGCAAGCAT TTGGTAGTGC CACCGGTGTC 
ACCAGATATT ACCCAGCTGC TCCCTGGAGA GCGCCAGATA AGATCGACCT TTATGATGTA 
CGACGAAGAC CCTGGTACAT TCAAGGAGCG TCGTCACCCA AAGACATGGT CATTCTTGTA 
GACGTGAGCG GCAGCGTCAG CGGGCTCACA TTAAAACTGA TCAAAGCTTC GGTCACAGAA 
ATGCTCGACA CACTCTCCGA TGATGATTAT GTCAACGTGG CCAGGTTTAA TGAGAAGGCA 
GAGGCAGTGG TGCCCTGTTT CGATCACCTG GTTCAGGCCA ATGTGCGCAA TAAGAAGATC 
TTCAAAGAGG CTGTGCAGCA GATGCAGGCC AAAGGGACCA CCGATTACAA ATCCGGCTTC 
CATTTCGCCT TCAACCAGCT CCTGAACAAG ACAAACGTTC CTCGAGCCAA CTGTAATAAA 
ATCATCATGT TGTTCACTGA CGGCGGAGAG GACCGAGCGC AGGACATCTT CGAGCAGTAC 
AACTGGCCTA ATAAGACGGT CCGCGTGTTC ACGTTCTCGG TGGGGCAGCA CAATTACGAT 
GTCACTCCGC TGCAGTGGAT TGCATGCTCT AATAAGGGAT ACTATTTTGA GATCCGCTCC 
ATCTGTGCTA TAAGGATTAA CACCCAGGAA TATCTGGATG TATTGGGTCG CCCGATGGTT 
CTGGCAGGCA GAATCGCCAA ACAAGTACAG TGGACAAACG TCTATCAGGA CGCCCTGGGT 
CTGGGTCTGG TGGTGACAGG GACAATGCCG GTGTTCAACC TTACTGTGAA CGGGGACTCT 
CAGAATCAGC TGATTTTAGG TGTGATGGGG GTCGACGTTC ATCTGGACGA GCTGAAGAGA 
CTCACACCAC AGTACAAGCT CGGAGCCAAT GGATACATCT TCGCCATTGA CCCGAACGGA 
TACGTGCTGA TGCATCCAAA CCTGCAGCCT CGAGAAGAGG ATTTACCGGA GCAGGTGACT 
CTGGACTTTC TGGATGCAGA AGTGGAAGAC AGCAGCAAAC AGGAGATTCG CCGGCAGATG 
ATTGACGGCC GATCGGGAGA TATGACCATT AAAACTCTAA TTAAATCAAT GGACGAGCGC 
TACATTGACG ACGTCACCCG GATGTACACC TGGACGCCCA TCAACGACAC AGACTACAGT 
CTAGGTTTGG TACTGCCTCC GTACAGTGAA CATTACATCC AAGCTGATCT GAGTGATGTC 
ATGCTCCAGC TGCAGTATTT ACAGTCTCTG CTGCCCAACT CTTTCGAGTC TGCCGGTCAT 
GTGTTTCTCG CTCCCAGAGA TTATTGCAGG CGCCTGCACA TCTCTGAAAA CAACACACAG 
TTCCTGGTGG ACTTCCTGTC TCTGATGGTG GAGATCTCAC CGGAGTCTGA AGACTGTGAT 
CAGGGTCTCA TTCATAACCT GATCTTGGAC TCCGGGATCC TCTGGCAGCT CGCCACACGC 
GTTTGGCAGA ATAAAGACCT GGGCACGTAT GGCTTTCTGG CTCTGTTTGC GTCCACCAAT 
GGAGGCGTCA CACGTGTTTA CCCCAACATG GCGGCTGAAT CATGGGATGA AGATCCTGAA 
CCACTAAACT CAAACTTCTA CCGGCGCAGT CTGGATAACA AAGGCTACAT CTTCAGAGCG 
CCGACACGAA CCTCGATGGA TGATCTGCTG ATCTCAGAGA ATGGCACTGT GGGGATTCTG 
GTTACCACCG CCGTAGAGGT TGCATTTGGG AGCAAAACCA TGAAGCCTGC AGTGGTCGGC 
GTTAAGCTGG ATTTGGAGGC TTGGGTCGAC AAGTTCAAGA TCTTGGCCAG CAACGTGTCC 
GACAGCAGAC AGGCCGCACA TAAGTGCGGC CCGTCCAGAT CCTGTGAGAT GGACTGTGAA 
GTGAACAGCG ATGATTTGCT GTGCTATCTG ATCGATGATG GCGGTTTCCT GGTGATGTCC 
AATCAGAGAG ATCACTGGAA GAAGGTTGGG CTGTTTTTCG GTGAGGTCGA CCCGTACCTG 
ATGTTCGCCC TCTACAACAA CTCAATCTAC TCCCGTCATC AGACCTTCCA GTATCAGTCG 
GCCTGCGAAC CCACAGCCAG CAGCCACACA GGAGCCGCAC ACAGAGGATT CTACGTGCCC 
TCCATAGCGG ACTTTCTCAG CCTAGCCTGG TGGACGTCTG CAGCCGCGTG GTCTATTTTG 
CAGCAGTTCT TGTATGGAGT CATGTACAGC AGCTGGTTCA CCACAGCAGA CGTCTGGGCT 
GAGAGTGCAG ACAGTAAAGG GAGCAGCAGC TGTGTGACGG TTTACAGCCA GTTCTACTTC 
ACCAACACCA CCAACTCCTT CAACGTCCTG CAGGACTGCG GCAACTGCTC CAGGTTGTTC 
CATGCCAAGC GTATAGAAAA CACAAACCTT TTGTTTGTGG TGGCTGAGAC ACTGCCCTGC 
AGCTCCTGCG AGATCGAGAA ACTCCTGCCT GTCAGAACCA AGTCTCAAGA AGAAAACCCA 
TGTGATGTCT TAAACTCGGC CCGCTATAGG AAAGGACCTG ACAAATGCTT CGACTACAAC 
TCAGAGGAGA ACACATCACA GTGTGGCAGC GCTCACTCTT TACACTGTCC GCTGCCAGCT 
CTGCTCTTGG TTCAGTTATT TTTGATTTAC CTCATTCTAC GATCGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009294387.1
CDS	207..3434
Translation	MARWAHACLLLLLMVILGGSWTAVNALTFPQHYTIMHWARRIEQEIDRVLQHISGAQQLKGIYLEKKKHFSLVRNNPRDIVEKVATDIERLLAKKRKALERLASEAERLQKEHRWQDGIKDGEDIDSQMSLKLDFVYDPSFKNQVNYSHTAVQIPTDIYKGAPVILNELNWTQALERVFIENSRDDPSLLWQAFGSATGVTRYYPAAPWRAPDKIDLYDVRRRPWYIQGASSPKDMVILVDVSGSVSGLTLKLIKASVTEMLDTLSDDDYVNVARFNEKAEAVVPCFDHLVQANVRNKKIFKEAVQQMQAKGTTDYKSGFHFAFNQLLNKTNVPRANCNKIIMLFTDGGEDRAQDIFEQYNWPNKTVRVFTFSVGQHNYDVTPLQWIACSNKGYYFEIRSICAIRINTQEYLDVLGRPMVLAGRIAKQVQWTNVYQDALGLGLVVTGTMPVFNLTVNGDSQNQLILGVMGVDVHLDELKRLTPQYKLGANGYIFAIDPNGYVLMHPNLQPREEDLPEQVTLDFLDAEVEDSSKQEIRRQMIDGRSGDMTIKTLIKSMDERYIDDVTRMYTWTPINDTDYSLGLVLPPYSEHYIQADLSDVMLQLQYLQSLLPNSFESAGHVFLAPRDYCRRLHISENNTQFLVDFLSLMVEISPESEDCDQGLIHNLILDSGILWQLATRVWQNKDLGTYGFLALFASTNGGVTRVYPNMAAESWDEDPEPLNSNFYRRSLDNKGYIFRAPTRTSMDDLLISENGTVGILVTTAVEVAFGSKTMKPAVVGVKLDLEAWVDKFKILASNVSDSRQAAHKCGPSRSCEMDCEVNSDDLLCYLIDDGGFLVMSNQRDHWKKVGLFFGEVDPYLMFALYNNSIYSRHQTFQYQSACEPTASSHTGAAHRGFYVPSIADFLSLAWWTSAAAWSILQQFLYGVMYSSWFTTADVWAESADSKGSSSCVTVYSQFYFTNTTNSFNVLQDCGNCSRLFHAKRIENTNLLFVVAETLPCSSCEIEKLLPVRTKSQEENPCDVLNSARYRKGPDKCFDYNSEENTSQCGSAHSLHCPLPALLLVQLFLIYLILRS

Target ORF information:

RefSeq Version	XM_009296112.2
Organism	Danio rerio(zebrafish)
Definition	Danio rerio calcium channel, voltage-dependent, alpha 2/delta subunit 2b (cacna2d2b), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009296112.2

ORF Insert Sequence:

ATGGCGAGAT GGGCTCACGC GTGTCTGCTG CTGCTGCTGA TGGTGATTCT CGGCGGATCC 
TGGACCGCAG TGAACGCGCT CACATTTCCT CAGCATTACA CGATTATGCA CTGGGCTCGA 
CGCATTGAAC AGGAGATCGA CAGAGTTTTG CAGCATATTA GTGGCGCTCA GCAACTGAAG 
GGGATCTACT TGGAAAAGAA AAAGCACTTC AGTCTGGTCA GAAACAACCC TCGAGACATT 
GTAGAGAAAG TAGCGACTGA CATCGAGAGA CTGCTTGCCA AGAAACGCAA AGCGCTGGAG 
AGACTGGCGA GTGAAGCGGA GCGTCTTCAG AAAGAGCATC GCTGGCAGGA TGGAATCAAG 
GACGGTGAGG ACATTGACTC TCAGATGTCC TTGAAACTGG ATTTTGTGTA CGACCCAAGC 
TTCAAAAACC AAGTGAATTA CTCGCACACT GCCGTCCAGA TCCCCACAGA CATCTACAAA 
GGCGCTCCAG TGATTCTGAA TGAGCTGAAC TGGACGCAGG CGCTAGAGCG AGTCTTCATT 
GAGAACAGCA GAGATGATCC CTCATTGCTT TGGCAAGCAT TTGGTAGTGC CACCGGTGTC 
ACCAGATATT ACCCAGCTGC TCCCTGGAGA GCGCCAGATA AGATCGACCT TTATGATGTA 
CGACGAAGAC CCTGGTACAT TCAAGGAGCG TCGTCACCCA AAGACATGGT CATTCTTGTA 
GACGTGAGCG GCAGCGTCAG CGGGCTCACA TTAAAACTGA TCAAAGCTTC GGTCACAGAA 
ATGCTCGACA CACTCTCCGA TGATGATTAT GTCAACGTGG CCAGGTTTAA TGAGAAGGCA 
GAGGCAGTGG TGCCCTGTTT CGATCACCTG GTTCAGGCCA ATGTGCGCAA TAAGAAGATC 
TTCAAAGAGG CTGTGCAGCA GATGCAGGCC AAAGGGACCA CCGATTACAA ATCCGGCTTC 
CATTTCGCCT TCAACCAGCT CCTGAACAAG ACAAACGTTC CTCGAGCCAA CTGTAATAAA 
ATCATCATGT TGTTCACTGA CGGCGGAGAG GACCGAGCGC AGGACATCTT CGAGCAGTAC 
AACTGGCCTA ATAAGACGGT CCGCGTGTTC ACGTTCTCGG TGGGGCAGCA CAATTACGAT 
GTCACTCCGC TGCAGTGGAT TGCATGCTCT AATAAGGGAT ACTATTTTGA GATCCGCTCC 
ATCTGTGCTA TAAGGATTAA CACCCAGGAA TATCTGGATG TATTGGGTCG CCCGATGGTT 
CTGGCAGGCA GAATCGCCAA ACAAGTACAG TGGACAAACG TCTATCAGGA CGCCCTGGGT 
CTGGGTCTGG TGGTGACAGG GACAATGCCG GTGTTCAACC TTACTGTGAA CGGGGACTCT 
CAGAATCAGC TGATTTTAGG TGTGATGGGG GTCGACGTTC ATCTGGACGA GCTGAAGAGA 
CTCACACCAC AGTACAAGCT CGGAGCCAAT GGATACATCT TCGCCATTGA CCCGAACGGA 
TACGTGCTGA TGCATCCAAA CCTGCAGCCT CGAGAAGAGG ATTTACCGGA GCAGGTGACT 
CTGGACTTTC TGGATGCAGA AGTGGAAGAC AGCAGCAAAC AGGAGATTCG CCGGCAGATG 
ATTGACGGCC GATCGGGAGA TATGACCATT AAAACTCTAA TTAAATCAAT GGACGAGCGC 
TACATTGACG ACGTCACCCG GATGTACACC TGGACGCCCA TCAACGACAC AGACTACAGT 
CTAGGTTTGG TACTGCCTCC GTACAGTGAA CATTACATCC AAGCTGATCT GAGTGATGTC 
ATGCTCCAGC TGCAGTATTT ACAGTCTCTG CTGCCCAACT CTTTCGAGTC TGCCGGTCAT 
GTGTTTCTCG CTCCCAGAGA TTATTGCAGG CGCCTGCACA TCTCTGAAAA CAACACACAG 
TTCCTGGTGG ACTTCCTGTC TCTGATGGTG GAGATCTCAC CGGAGTCTGA AGACTGTGAT 
CAGGGTCTCA TTCATAACCT GATCTTGGAC TCCGGGATCC TCTGGCAGCT CGCCACACGC 
GTTTGGCAGA ATAAAGACCT GGGCACGTAT GGCTTTCTGG CTCTGTTTGC GTCCACCAAT 
GGAGGCGTCA CACGTGTTTA CCCCAACATG GCGGCTGAAT CATGGGATGA AGATCCTGAA 
CCACTAAACT CAAACTTCTA CCGGCGCAGT CTGGATAACA AAGGCTACAT CTTCAGAGCG 
CCGACACGAA CCTCGATGGA TGATCTGCTG ATCTCAGAGA ATGGCACTGT GGGGATTCTG 
GTTACCACCG CCGTAGAGGT TGCATTTGGG AGCAAAACCA TGAAGCCTGC AGTGGTCGGC 
GTTAAGCTGG ATTTGGAGGC TTGGGTCGAC AAGTTCAAGA TCTTGGCCAG CAACGTGTCC 
GACAGCAGAC AGGCCGCACA TAAGTGCGGC CCGTCCAGAT CCTGTGAGAT GGACTGTGAA 
GTGAACAGCG ATGATTTGCT GTGCTATCTG ATCGATGATG GCGGTTTCCT GGTGATGTCC 
AATCAGAGAG ATCACTGGAA GAAGGTTGGG CTGTTTTTCG GTGAGGTCGA CCCGTACCTG 
ATGTTCGCCC TCTACAACAA CTCAATCTAC TCCCGTCATC AGACCTTCCA GTATCAGTCG 
GCCTGCGAAC CCACAGCCAG CAGCCACACA GGAGCCGCAC ACAGAGGATT CTACGTGCCC 
TCCATAGCGG ACTTTCTCAG CCTAGCCTGG TGGACGTCTG CAGCCGCGTG GTCTATTTTG 
CAGCAGTTCT TGTATGGAGT CATGTACAGC AGCTGGTTCA CCACAGCAGA CGTCTGGGCT 
GAGAGTGCAG ACAGTAAAGG GAGCAGCAGC TGTGTGACGG TTTACAGCCA GTTCTACTTC 
ACCAACACCA CCAACTCCTT CAACGTCCTG CAGGACTGCG GCAACTGCTC CAGGTTGTTC 
CATGCCAAGC GTATAGAAAA CACAAACCTT TTGTTTGTGG TGGCTGAGAC ACTGCCCTGC 
AGCTCCTGCG AGATCGAGAA ACTCCTGCCT GTCAGAACCA AGTCTCAAGA AGAAAACCCA 
TGTGATGTCT TAAACTCGGC CCGCTATAGG AAAGGACCTG ACAAATGCTT CGACTACAAC 
TCAGAGGAGA ACACATCACA GTGTGGCAGC GCTCACTCTT TACACTGTCC GCTGCCAGCT 
CTGCTCTTGG TTCAGTTATT TTTGATTTAC CTCATTCTAC GATCGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

cacna2d2b ( XM_009296111.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009294386.1 Danio rerio calcium channel, voltage-dependent, alpha 2/delta subunit 2b (cacna2d2b), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ODa45532
Clone ID Related Accession (Same CDS sequence)	XM_009296111.1 , XM_009296111.2
Accession Version	XM_009296111.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3270bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	calcium channel, voltage-dependent, alpha 2/delta subunit 2 isoform X2
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007133.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGAGAT GGGCTCACGC GTGTCTGCTG CTGCTGCTGA TGGTGATTCT CGGCGGATCC 
TGGACCGCAG TGAACGCGCT CACATTTCCT CAGCATTACA CGATTATGCA CTGGGCTCGA 
CGCATTGAAC AGGAGATCGA CAGAGTTTTG CAGCATATTA GTGGCGCTCA GCAACTGAAG 
GGGATCTACT TGGAAAAGAA AAAGCACTTC AGTCTGGTCA GAAACAACCC TCGAGACATT 
GTAGAGAAAG TAGCGACTGA CATCGAGAGA CTGCTTGCCA AGAAACGCAA AGCGCTGGAG 
AGACTGGCGA GTGAAGCGGA GCGTCTTCAG AAAGAGCATC GCTGGCAGGA TGGAATCAAG 
GAGGAAAATA TTGAGTATTA TAACTCTAAA GCAGAGATGG ATTATGACGG TGAGGACATT 
GACTCTCAGA TGTCCTTGAA ACTGGATTTT GTGTACGACC CAAGCTTCAA AAACCAAGTG 
AATTACTCGC ACACTGCCGT CCAGATCCCC ACAGACATCT ACAAAGGCGC TCCAGTGATT 
CTGAATGAGC TGAACTGGAC GCAGGCGCTA GAGCGAGTCT TCATTGAGAA CAGCAGAGAT 
GATCCCTCAT TGCTTTGGCA AGCATTTGGT AGTGCCACCG GTGTCACCAG ATATTACCCA 
GCTGCTCCCT GGAGAGCGCC AGATAAGATC GACCTTTATG ATGTACGACG AAGACCCTGG 
TACATTCAAG GAGCGTCGTC ACCCAAAGAC ATGGTCATTC TTGTAGACGT GAGCGGCAGC 
GTCAGCGGGC TCACATTAAA ACTGATCAAA GCTTCGGTCA CAGAAATGCT CGACACACTC 
TCCGATGATG ATTATGTCAA CGTGGCCAGG TTTAATGAGA AGGCAGAGGC AGTGGTGCCC 
TGTTTCGATC ACCTGGTTCA GGCCAATGTG CGCAATAAGA AGATCTTCAA AGAGGCTGTG 
CAGCAGATGC AGGCCAAAGG GACCACCGAT TACAAATCCG GCTTCCATTT CGCCTTCAAC 
CAGCTCCTGA ACAAGACAAA CGTTCCTCGA GCCAACTGTA ATAAAATCAT CATGTTGTTC 
ACTGACGGCG GAGAGGACCG AGCGCAGGAC ATCTTCGAGC AGTACAACTG GCCTAATAAG 
ACGGTCCGCG TGTTCACGTT CTCGGTGGGG CAGCACAATT ACGATGTCAC TCCGCTGCAG 
TGGATTGCAT GCTCTAATAA GGGATACTAT TTTGAGATCC GCTCCATCTG TGCTATAAGG 
ATTAACACCC AGGAATATCT GGATGTATTG GGTCGCCCGA TGGTTCTGGC AGGCAGAATC 
GCCAAACAAG TACAGTGGAC AAACGTCTAT CAGGACGCCC TGGGTCTGGG TCTGGTGGTG 
ACAGGGACAA TGCCGGTGTT CAACCTTACT GTGAACGGGG ACTCTCAGAA TCAGCTGATT 
TTAGGTGTGA TGGGGGTCGA CGTTCATCTG GACGAGCTGA AGAGACTCAC ACCACAGTAC 
AAGCTCGGAG CCAATGGATA CATCTTCGCC ATTGACCCGA ACGGATACGT GCTGATGCAT 
CCAAACCTGC AGCCTCGAGA AGAGGATTTA CCGGAGCAGG TGACTCTGGA CTTTCTGGAT 
GCAGAAGTGG AAGACAGCAG CAAACAGGAG ATTCGCCGGC AGATGATTGA CGGCCGATCG 
GGAGATATGA CCATTAAAAC TCTAATTAAA TCAATGGACG AGCGCTACAT TGACGACGTC 
ACCCGGATGT ACACCTGGAC GCCCATCAAC GACACAGACT ACAGTCTAGG TTTGGTACTG 
CCTCCGTACA GTGAACATTA CATCCAAGCT GATCTGAGTG ATGTCATGCT CCAGCTGCAG 
TATTTACAGT CTCTGCTGCC CAACTCTTTC GAGTCTGCCG GTCATGTGTT TCTCGCTCCC 
AGAGATTATT GCAGGCGCCT GCACATCTCT GAAAACAACA CACAGTTCCT GGTGGACTTC 
CTGTCTCTGA TGGTGGAGAT CTCACCGGAG TCTGAAGACT GTGATCAGGG TCTCATTCAT 
AACCTGATCT TGGACTCCGG GATCCTCTGG CAGCTCGCCA CACGCGTTTG GCAGAATAAA 
GACCTGGGCA CGTATGGCTT TCTGGCTCTG TTTGCGTCCA CCAATGGAGG CGTCACACGT 
GTTTACCCCA ACATGGCGGC TGAATCATGG GATGAAGATC CTGAACCACT AAACTCAAAC 
TTCTACCGGC GCAGTCTGGA TAACAAAGGC TACATCTTCA GAGCGCCGAC ACGAACCTCG 
ATGGATGATC TGCTGATCTC AGAGAATGGC ACTGTGGGGA TTCTGGTTAC CACCGCCGTA 
GAGGTTGCAT TTGGGAGCAA AACCATGAAG CCTGCAGTGG TCGGCGTTAA GCTGGATTTG 
GAGGCTTGGG TCGACAAGTT CAAGATCTTG GCCAGCAACG TGTCCGACAG CAGACAGGCC 
GCACATAAGT GCGGCCCGTC CAGATCCTGT GAGATGGACT GTGAAGTGAA CAGCGATGAT 
TTGCTGTGCT ATCTGATCGA TGATGGCGGT TTCCTGGTGA TGTCCAATCA GAGAGATCAC 
TGGAAGAAGG TTGGGCTGTT TTTCGGTGAG GTCGACCCGT ACCTGATGTT CGCCCTCTAC 
AACAACTCAA TCTACTCCCG TCATCAGACC TTCCAGTATC AGTCGGCCTG CGAACCCACA 
GCCAGCAGCC ACACAGGAGC CGCACACAGA GGATTCTACG TGCCCTCCAT AGCGGACTTT 
CTCAGCCTAG CCTGGTGGAC GTCTGCAGCC GCGTGGTCTA TTTTGCAGCA GTTCTTGTAT 
GGAGTCATGT ACAGCAGCTG GTTCACCACA GACGTCTGGG CTGAGAGTGC AGACAGTAAA 
GGGAGCAGCA GCTGTGTGAC GGTTTACAGC CAGTTCTACT TCACCAACAC CACCAACTCC 
TTCAACGTCC TGCAGGACTG CGGCAACTGC TCCAGGTTGT TCCATGCCAA GCGTATAGAA 
AACACAAACC TTTTGTTTGT GGTGGCTGAG ACACTGCCCT GCAGCTCCTG CGAGATCGAG 
AAACTCCTGC CTGTCAGAAC CAAGTCTCAA GAAGAAAACC CATGTGATGT CTTAAACTCG 
GCCCGCTATA GGAAAGGACC TGACAAATGC TTCGACTACA ACTCAGAGGA GAACACATCA 
CAGTGTGGCA GCGCTCACTC TTTACACTGT CCGCTGCCAG CTCTGCTCTT GGTTCAGTTA 
TTTTTGATTT ACCTCATTCT ACGATCGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009294386.1
CDS	190..3459
Translation	MARWAHACLLLLLMVILGGSWTAVNALTFPQHYTIMHWARRIEQEIDRVLQHISGAQQLKGIYLEKKKHFSLVRNNPRDIVEKVATDIERLLAKKRKALERLASEAERLQKEHRWQDGIKEENIEYYNSKAEMDYDGEDIDSQMSLKLDFVYDPSFKNQVNYSHTAVQIPTDIYKGAPVILNELNWTQALERVFIENSRDDPSLLWQAFGSATGVTRYYPAAPWRAPDKIDLYDVRRRPWYIQGASSPKDMVILVDVSGSVSGLTLKLIKASVTEMLDTLSDDDYVNVARFNEKAEAVVPCFDHLVQANVRNKKIFKEAVQQMQAKGTTDYKSGFHFAFNQLLNKTNVPRANCNKIIMLFTDGGEDRAQDIFEQYNWPNKTVRVFTFSVGQHNYDVTPLQWIACSNKGYYFEIRSICAIRINTQEYLDVLGRPMVLAGRIAKQVQWTNVYQDALGLGLVVTGTMPVFNLTVNGDSQNQLILGVMGVDVHLDELKRLTPQYKLGANGYIFAIDPNGYVLMHPNLQPREEDLPEQVTLDFLDAEVEDSSKQEIRRQMIDGRSGDMTIKTLIKSMDERYIDDVTRMYTWTPINDTDYSLGLVLPPYSEHYIQADLSDVMLQLQYLQSLLPNSFESAGHVFLAPRDYCRRLHISENNTQFLVDFLSLMVEISPESEDCDQGLIHNLILDSGILWQLATRVWQNKDLGTYGFLALFASTNGGVTRVYPNMAAESWDEDPEPLNSNFYRRSLDNKGYIFRAPTRTSMDDLLISENGTVGILVTTAVEVAFGSKTMKPAVVGVKLDLEAWVDKFKILASNVSDSRQAAHKCGPSRSCEMDCEVNSDDLLCYLIDDGGFLVMSNQRDHWKKVGLFFGEVDPYLMFALYNNSIYSRHQTFQYQSACEPTASSHTGAAHRGFYVPSIADFLSLAWWTSAAAWSILQQFLYGVMYSSWFTTDVWAESADSKGSSSCVTVYSQFYFTNTTNSFNVLQDCGNCSRLFHAKRIENTNLLFVVAETLPCSSCEIEKLLPVRTKSQEENPCDVLNSARYRKGPDKCFDYNSEENTSQCGSAHSLHCPLPALLLVQLFLIYLILRS

Target ORF information:

RefSeq Version	XM_009296111.1
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio calcium channel, voltage-dependent, alpha 2/delta subunit 2b (cacna2d2b), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009296111.1

ORF Insert Sequence:

ATGGCGAGAT GGGCTCACGC GTGTCTGCTG CTGCTGCTGA TGGTGATTCT CGGCGGATCC 
TGGACCGCAG TGAACGCGCT CACATTTCCT CAGCATTACA CGATTATGCA CTGGGCTCGA 
CGCATTGAAC AGGAGATCGA CAGAGTTTTG CAGCATATTA GTGGCGCTCA GCAACTGAAG 
GGGATCTACT TGGAAAAGAA AAAGCACTTC AGTCTGGTCA GAAACAACCC TCGAGACATT 
GTAGAGAAAG TAGCGACTGA CATCGAGAGA CTGCTTGCCA AGAAACGCAA AGCGCTGGAG 
AGACTGGCGA GTGAAGCGGA GCGTCTTCAG AAAGAGCATC GCTGGCAGGA TGGAATCAAG 
GAGGAAAATA TTGAGTATTA TAACTCTAAA GCAGAGATGG ATTATGACGG TGAGGACATT 
GACTCTCAGA TGTCCTTGAA ACTGGATTTT GTGTACGACC CAAGCTTCAA AAACCAAGTG 
AATTACTCGC ACACTGCCGT CCAGATCCCC ACAGACATCT ACAAAGGCGC TCCAGTGATT 
CTGAATGAGC TGAACTGGAC GCAGGCGCTA GAGCGAGTCT TCATTGAGAA CAGCAGAGAT 
GATCCCTCAT TGCTTTGGCA AGCATTTGGT AGTGCCACCG GTGTCACCAG ATATTACCCA 
GCTGCTCCCT GGAGAGCGCC AGATAAGATC GACCTTTATG ATGTACGACG AAGACCCTGG 
TACATTCAAG GAGCGTCGTC ACCCAAAGAC ATGGTCATTC TTGTAGACGT GAGCGGCAGC 
GTCAGCGGGC TCACATTAAA ACTGATCAAA GCTTCGGTCA CAGAAATGCT CGACACACTC 
TCCGATGATG ATTATGTCAA CGTGGCCAGG TTTAATGAGA AGGCAGAGGC AGTGGTGCCC 
TGTTTCGATC ACCTGGTTCA GGCCAATGTG CGCAATAAGA AGATCTTCAA AGAGGCTGTG 
CAGCAGATGC AGGCCAAAGG GACCACCGAT TACAAATCCG GCTTCCATTT CGCCTTCAAC 
CAGCTCCTGA ACAAGACAAA CGTTCCTCGA GCCAACTGTA ATAAAATCAT CATGTTGTTC 
ACTGACGGCG GAGAGGACCG AGCGCAGGAC ATCTTCGAGC AGTACAACTG GCCTAATAAG 
ACGGTCCGCG TGTTCACGTT CTCGGTGGGG CAGCACAATT ACGATGTCAC TCCGCTGCAG 
TGGATTGCAT GCTCTAATAA GGGATACTAT TTTGAGATCC GCTCCATCTG TGCTATAAGG 
ATTAACACCC AGGAATATCT GGATGTATTG GGTCGCCCGA TGGTTCTGGC AGGCAGAATC 
GCCAAACAAG TACAGTGGAC AAACGTCTAT CAGGACGCCC TGGGTCTGGG TCTGGTGGTG 
ACAGGGACAA TGCCGGTGTT CAACCTTACT GTGAACGGGG ACTCTCAGAA TCAGCTGATT 
TTAGGTGTGA TGGGGGTCGA CGTTCATCTG GACGAGCTGA AGAGACTCAC ACCACAGTAC 
AAGCTCGGAG CCAATGGATA CATCTTCGCC ATTGACCCGA ACGGATACGT GCTGATGCAT 
CCAAACCTGC AGCCTCGAGA AGAGGATTTA CCGGAGCAGG TGACTCTGGA CTTTCTGGAT 
GCAGAAGTGG AAGACAGCAG CAAACAGGAG ATTCGCCGGC AGATGATTGA CGGCCGATCG 
GGAGATATGA CCATTAAAAC TCTAATTAAA TCAATGGACG AGCGCTACAT TGACGACGTC 
ACCCGGATGT ACACCTGGAC GCCCATCAAC GACACAGACT ACAGTCTAGG TTTGGTACTG 
CCTCCGTACA GTGAACATTA CATCCAAGCT GATCTGAGTG ATGTCATGCT CCAGCTGCAG 
TATTTACAGT CTCTGCTGCC CAACTCTTTC GAGTCTGCCG GTCATGTGTT TCTCGCTCCC 
AGAGATTATT GCAGGCGCCT GCACATCTCT GAAAACAACA CACAGTTCCT GGTGGACTTC 
CTGTCTCTGA TGGTGGAGAT CTCACCGGAG TCTGAAGACT GTGATCAGGG TCTCATTCAT 
AACCTGATCT TGGACTCCGG GATCCTCTGG CAGCTCGCCA CACGCGTTTG GCAGAATAAA 
GACCTGGGCA CGTATGGCTT TCTGGCTCTG TTTGCGTCCA CCAATGGAGG CGTCACACGT 
GTTTACCCCA ACATGGCGGC TGAATCATGG GATGAAGATC CTGAACCACT AAACTCAAAC 
TTCTACCGGC GCAGTCTGGA TAACAAAGGC TACATCTTCA GAGCGCCGAC ACGAACCTCG 
ATGGATGATC TGCTGATCTC AGAGAATGGC ACTGTGGGGA TTCTGGTTAC CACCGCCGTA 
GAGGTTGCAT TTGGGAGCAA AACCATGAAG CCTGCAGTGG TCGGCGTTAA GCTGGATTTG 
GAGGCTTGGG TCGACAAGTT CAAGATCTTG GCCAGCAACG TGTCCGACAG CAGACAGGCC 
GCACATAAGT GCGGCCCGTC CAGATCCTGT GAGATGGACT GTGAAGTGAA CAGCGATGAT 
TTGCTGTGCT ATCTGATCGA TGATGGCGGT TTCCTGGTGA TGTCCAATCA GAGAGATCAC 
TGGAAGAAGG TTGGGCTGTT TTTCGGTGAG GTCGACCCGT ACCTGATGTT CGCCCTCTAC 
AACAACTCAA TCTACTCCCG TCATCAGACC TTCCAGTATC AGTCGGCCTG CGAACCCACA 
GCCAGCAGCC ACACAGGAGC CGCACACAGA GGATTCTACG TGCCCTCCAT AGCGGACTTT 
CTCAGCCTAG CCTGGTGGAC GTCTGCAGCC GCGTGGTCTA TTTTGCAGCA GTTCTTGTAT 
GGAGTCATGT ACAGCAGCTG GTTCACCACA GACGTCTGGG CTGAGAGTGC AGACAGTAAA 
GGGAGCAGCA GCTGTGTGAC GGTTTACAGC CAGTTCTACT TCACCAACAC CACCAACTCC 
TTCAACGTCC TGCAGGACTG CGGCAACTGC TCCAGGTTGT TCCATGCCAA GCGTATAGAA 
AACACAAACC TTTTGTTTGT GGTGGCTGAG ACACTGCCCT GCAGCTCCTG CGAGATCGAG 
AAACTCCTGC CTGTCAGAAC CAAGTCTCAA GAAGAAAACC CATGTGATGT CTTAAACTCG 
GCCCGCTATA GGAAAGGACC TGACAAATGC TTCGACTACA ACTCAGAGGA GAACACATCA 
CAGTGTGGCA GCGCTCACTC TTTACACTGT CCGCTGCCAG CTCTGCTCTT GGTTCAGTTA 
TTTTTGATTT ACCTCATTCT ACGATCGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

cacna2d2b ( XM_009296111.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009294386.1 Danio rerio calcium channel, voltage-dependent, alpha 2/delta subunit 2b (cacna2d2b), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ODa45532
Clone ID Related Accession (Same CDS sequence)	XM_009296111.1 , XM_009296111.2
Accession Version	XM_009296111.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3270bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	calcium channel, voltage-dependent, alpha 2/delta subunit 2 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007133.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009296111.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGAGAT GGGCTCACGC GTGTCTGCTG CTGCTGCTGA TGGTGATTCT CGGCGGATCC 
TGGACCGCAG TGAACGCGCT CACATTTCCT CAGCATTACA CGATTATGCA CTGGGCTCGA 
CGCATTGAAC AGGAGATCGA CAGAGTTTTG CAGCATATTA GTGGCGCTCA GCAACTGAAG 
GGGATCTACT TGGAAAAGAA AAAGCACTTC AGTCTGGTCA GAAACAACCC TCGAGACATT 
GTAGAGAAAG TAGCGACTGA CATCGAGAGA CTGCTTGCCA AGAAACGCAA AGCGCTGGAG 
AGACTGGCGA GTGAAGCGGA GCGTCTTCAG AAAGAGCATC GCTGGCAGGA TGGAATCAAG 
GAGGAAAATA TTGAGTATTA TAACTCTAAA GCAGAGATGG ATTATGACGG TGAGGACATT 
GACTCTCAGA TGTCCTTGAA ACTGGATTTT GTGTACGACC CAAGCTTCAA AAACCAAGTG 
AATTACTCGC ACACTGCCGT CCAGATCCCC ACAGACATCT ACAAAGGCGC TCCAGTGATT 
CTGAATGAGC TGAACTGGAC GCAGGCGCTA GAGCGAGTCT TCATTGAGAA CAGCAGAGAT 
GATCCCTCAT TGCTTTGGCA AGCATTTGGT AGTGCCACCG GTGTCACCAG ATATTACCCA 
GCTGCTCCCT GGAGAGCGCC AGATAAGATC GACCTTTATG ATGTACGACG AAGACCCTGG 
TACATTCAAG GAGCGTCGTC ACCCAAAGAC ATGGTCATTC TTGTAGACGT GAGCGGCAGC 
GTCAGCGGGC TCACATTAAA ACTGATCAAA GCTTCGGTCA CAGAAATGCT CGACACACTC 
TCCGATGATG ATTATGTCAA CGTGGCCAGG TTTAATGAGA AGGCAGAGGC AGTGGTGCCC 
TGTTTCGATC ACCTGGTTCA GGCCAATGTG CGCAATAAGA AGATCTTCAA AGAGGCTGTG 
CAGCAGATGC AGGCCAAAGG GACCACCGAT TACAAATCCG GCTTCCATTT CGCCTTCAAC 
CAGCTCCTGA ACAAGACAAA CGTTCCTCGA GCCAACTGTA ATAAAATCAT CATGTTGTTC 
ACTGACGGCG GAGAGGACCG AGCGCAGGAC ATCTTCGAGC AGTACAACTG GCCTAATAAG 
ACGGTCCGCG TGTTCACGTT CTCGGTGGGG CAGCACAATT ACGATGTCAC TCCGCTGCAG 
TGGATTGCAT GCTCTAATAA GGGATACTAT TTTGAGATCC GCTCCATCTG TGCTATAAGG 
ATTAACACCC AGGAATATCT GGATGTATTG GGTCGCCCGA TGGTTCTGGC AGGCAGAATC 
GCCAAACAAG TACAGTGGAC AAACGTCTAT CAGGACGCCC TGGGTCTGGG TCTGGTGGTG 
ACAGGGACAA TGCCGGTGTT CAACCTTACT GTGAACGGGG ACTCTCAGAA TCAGCTGATT 
TTAGGTGTGA TGGGGGTCGA CGTTCATCTG GACGAGCTGA AGAGACTCAC ACCACAGTAC 
AAGCTCGGAG CCAATGGATA CATCTTCGCC ATTGACCCGA ACGGATACGT GCTGATGCAT 
CCAAACCTGC AGCCTCGAGA AGAGGATTTA CCGGAGCAGG TGACTCTGGA CTTTCTGGAT 
GCAGAAGTGG AAGACAGCAG CAAACAGGAG ATTCGCCGGC AGATGATTGA CGGCCGATCG 
GGAGATATGA CCATTAAAAC TCTAATTAAA TCAATGGACG AGCGCTACAT TGACGACGTC 
ACCCGGATGT ACACCTGGAC GCCCATCAAC GACACAGACT ACAGTCTAGG TTTGGTACTG 
CCTCCGTACA GTGAACATTA CATCCAAGCT GATCTGAGTG ATGTCATGCT CCAGCTGCAG 
TATTTACAGT CTCTGCTGCC CAACTCTTTC GAGTCTGCCG GTCATGTGTT TCTCGCTCCC 
AGAGATTATT GCAGGCGCCT GCACATCTCT GAAAACAACA CACAGTTCCT GGTGGACTTC 
CTGTCTCTGA TGGTGGAGAT CTCACCGGAG TCTGAAGACT GTGATCAGGG TCTCATTCAT 
AACCTGATCT TGGACTCCGG GATCCTCTGG CAGCTCGCCA CACGCGTTTG GCAGAATAAA 
GACCTGGGCA CGTATGGCTT TCTGGCTCTG TTTGCGTCCA CCAATGGAGG CGTCACACGT 
GTTTACCCCA ACATGGCGGC TGAATCATGG GATGAAGATC CTGAACCACT AAACTCAAAC 
TTCTACCGGC GCAGTCTGGA TAACAAAGGC TACATCTTCA GAGCGCCGAC ACGAACCTCG 
ATGGATGATC TGCTGATCTC AGAGAATGGC ACTGTGGGGA TTCTGGTTAC CACCGCCGTA 
GAGGTTGCAT TTGGGAGCAA AACCATGAAG CCTGCAGTGG TCGGCGTTAA GCTGGATTTG 
GAGGCTTGGG TCGACAAGTT CAAGATCTTG GCCAGCAACG TGTCCGACAG CAGACAGGCC 
GCACATAAGT GCGGCCCGTC CAGATCCTGT GAGATGGACT GTGAAGTGAA CAGCGATGAT 
TTGCTGTGCT ATCTGATCGA TGATGGCGGT TTCCTGGTGA TGTCCAATCA GAGAGATCAC 
TGGAAGAAGG TTGGGCTGTT TTTCGGTGAG GTCGACCCGT ACCTGATGTT CGCCCTCTAC 
AACAACTCAA TCTACTCCCG TCATCAGACC TTCCAGTATC AGTCGGCCTG CGAACCCACA 
GCCAGCAGCC ACACAGGAGC CGCACACAGA GGATTCTACG TGCCCTCCAT AGCGGACTTT 
CTCAGCCTAG CCTGGTGGAC GTCTGCAGCC GCGTGGTCTA TTTTGCAGCA GTTCTTGTAT 
GGAGTCATGT ACAGCAGCTG GTTCACCACA GACGTCTGGG CTGAGAGTGC AGACAGTAAA 
GGGAGCAGCA GCTGTGTGAC GGTTTACAGC CAGTTCTACT TCACCAACAC CACCAACTCC 
TTCAACGTCC TGCAGGACTG CGGCAACTGC TCCAGGTTGT TCCATGCCAA GCGTATAGAA 
AACACAAACC TTTTGTTTGT GGTGGCTGAG ACACTGCCCT GCAGCTCCTG CGAGATCGAG 
AAACTCCTGC CTGTCAGAAC CAAGTCTCAA GAAGAAAACC CATGTGATGT CTTAAACTCG 
GCCCGCTATA GGAAAGGACC TGACAAATGC TTCGACTACA ACTCAGAGGA GAACACATCA 
CAGTGTGGCA GCGCTCACTC TTTACACTGT CCGCTGCCAG CTCTGCTCTT GGTTCAGTTA 
TTTTTGATTT ACCTCATTCT ACGATCGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009294386.1
CDS	207..3476
Translation	MARWAHACLLLLLMVILGGSWTAVNALTFPQHYTIMHWARRIEQEIDRVLQHISGAQQLKGIYLEKKKHFSLVRNNPRDIVEKVATDIERLLAKKRKALERLASEAERLQKEHRWQDGIKEENIEYYNSKAEMDYDGEDIDSQMSLKLDFVYDPSFKNQVNYSHTAVQIPTDIYKGAPVILNELNWTQALERVFIENSRDDPSLLWQAFGSATGVTRYYPAAPWRAPDKIDLYDVRRRPWYIQGASSPKDMVILVDVSGSVSGLTLKLIKASVTEMLDTLSDDDYVNVARFNEKAEAVVPCFDHLVQANVRNKKIFKEAVQQMQAKGTTDYKSGFHFAFNQLLNKTNVPRANCNKIIMLFTDGGEDRAQDIFEQYNWPNKTVRVFTFSVGQHNYDVTPLQWIACSNKGYYFEIRSICAIRINTQEYLDVLGRPMVLAGRIAKQVQWTNVYQDALGLGLVVTGTMPVFNLTVNGDSQNQLILGVMGVDVHLDELKRLTPQYKLGANGYIFAIDPNGYVLMHPNLQPREEDLPEQVTLDFLDAEVEDSSKQEIRRQMIDGRSGDMTIKTLIKSMDERYIDDVTRMYTWTPINDTDYSLGLVLPPYSEHYIQADLSDVMLQLQYLQSLLPNSFESAGHVFLAPRDYCRRLHISENNTQFLVDFLSLMVEISPESEDCDQGLIHNLILDSGILWQLATRVWQNKDLGTYGFLALFASTNGGVTRVYPNMAAESWDEDPEPLNSNFYRRSLDNKGYIFRAPTRTSMDDLLISENGTVGILVTTAVEVAFGSKTMKPAVVGVKLDLEAWVDKFKILASNVSDSRQAAHKCGPSRSCEMDCEVNSDDLLCYLIDDGGFLVMSNQRDHWKKVGLFFGEVDPYLMFALYNNSIYSRHQTFQYQSACEPTASSHTGAAHRGFYVPSIADFLSLAWWTSAAAWSILQQFLYGVMYSSWFTTDVWAESADSKGSSSCVTVYSQFYFTNTTNSFNVLQDCGNCSRLFHAKRIENTNLLFVVAETLPCSSCEIEKLLPVRTKSQEENPCDVLNSARYRKGPDKCFDYNSEENTSQCGSAHSLHCPLPALLLVQLFLIYLILRS

Target ORF information:

RefSeq Version	XM_009296111.2
Organism	Danio rerio(zebrafish)
Definition	Danio rerio calcium channel, voltage-dependent, alpha 2/delta subunit 2b (cacna2d2b), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009296111.2

ORF Insert Sequence:

ATGGCGAGAT GGGCTCACGC GTGTCTGCTG CTGCTGCTGA TGGTGATTCT CGGCGGATCC 
TGGACCGCAG TGAACGCGCT CACATTTCCT CAGCATTACA CGATTATGCA CTGGGCTCGA 
CGCATTGAAC AGGAGATCGA CAGAGTTTTG CAGCATATTA GTGGCGCTCA GCAACTGAAG 
GGGATCTACT TGGAAAAGAA AAAGCACTTC AGTCTGGTCA GAAACAACCC TCGAGACATT 
GTAGAGAAAG TAGCGACTGA CATCGAGAGA CTGCTTGCCA AGAAACGCAA AGCGCTGGAG 
AGACTGGCGA GTGAAGCGGA GCGTCTTCAG AAAGAGCATC GCTGGCAGGA TGGAATCAAG 
GAGGAAAATA TTGAGTATTA TAACTCTAAA GCAGAGATGG ATTATGACGG TGAGGACATT 
GACTCTCAGA TGTCCTTGAA ACTGGATTTT GTGTACGACC CAAGCTTCAA AAACCAAGTG 
AATTACTCGC ACACTGCCGT CCAGATCCCC ACAGACATCT ACAAAGGCGC TCCAGTGATT 
CTGAATGAGC TGAACTGGAC GCAGGCGCTA GAGCGAGTCT TCATTGAGAA CAGCAGAGAT 
GATCCCTCAT TGCTTTGGCA AGCATTTGGT AGTGCCACCG GTGTCACCAG ATATTACCCA 
GCTGCTCCCT GGAGAGCGCC AGATAAGATC GACCTTTATG ATGTACGACG AAGACCCTGG 
TACATTCAAG GAGCGTCGTC ACCCAAAGAC ATGGTCATTC TTGTAGACGT GAGCGGCAGC 
GTCAGCGGGC TCACATTAAA ACTGATCAAA GCTTCGGTCA CAGAAATGCT CGACACACTC 
TCCGATGATG ATTATGTCAA CGTGGCCAGG TTTAATGAGA AGGCAGAGGC AGTGGTGCCC 
TGTTTCGATC ACCTGGTTCA GGCCAATGTG CGCAATAAGA AGATCTTCAA AGAGGCTGTG 
CAGCAGATGC AGGCCAAAGG GACCACCGAT TACAAATCCG GCTTCCATTT CGCCTTCAAC 
CAGCTCCTGA ACAAGACAAA CGTTCCTCGA GCCAACTGTA ATAAAATCAT CATGTTGTTC 
ACTGACGGCG GAGAGGACCG AGCGCAGGAC ATCTTCGAGC AGTACAACTG GCCTAATAAG 
ACGGTCCGCG TGTTCACGTT CTCGGTGGGG CAGCACAATT ACGATGTCAC TCCGCTGCAG 
TGGATTGCAT GCTCTAATAA GGGATACTAT TTTGAGATCC GCTCCATCTG TGCTATAAGG 
ATTAACACCC AGGAATATCT GGATGTATTG GGTCGCCCGA TGGTTCTGGC AGGCAGAATC 
GCCAAACAAG TACAGTGGAC AAACGTCTAT CAGGACGCCC TGGGTCTGGG TCTGGTGGTG 
ACAGGGACAA TGCCGGTGTT CAACCTTACT GTGAACGGGG ACTCTCAGAA TCAGCTGATT 
TTAGGTGTGA TGGGGGTCGA CGTTCATCTG GACGAGCTGA AGAGACTCAC ACCACAGTAC 
AAGCTCGGAG CCAATGGATA CATCTTCGCC ATTGACCCGA ACGGATACGT GCTGATGCAT 
CCAAACCTGC AGCCTCGAGA AGAGGATTTA CCGGAGCAGG TGACTCTGGA CTTTCTGGAT 
GCAGAAGTGG AAGACAGCAG CAAACAGGAG ATTCGCCGGC AGATGATTGA CGGCCGATCG 
GGAGATATGA CCATTAAAAC TCTAATTAAA TCAATGGACG AGCGCTACAT TGACGACGTC 
ACCCGGATGT ACACCTGGAC GCCCATCAAC GACACAGACT ACAGTCTAGG TTTGGTACTG 
CCTCCGTACA GTGAACATTA CATCCAAGCT GATCTGAGTG ATGTCATGCT CCAGCTGCAG 
TATTTACAGT CTCTGCTGCC CAACTCTTTC GAGTCTGCCG GTCATGTGTT TCTCGCTCCC 
AGAGATTATT GCAGGCGCCT GCACATCTCT GAAAACAACA CACAGTTCCT GGTGGACTTC 
CTGTCTCTGA TGGTGGAGAT CTCACCGGAG TCTGAAGACT GTGATCAGGG TCTCATTCAT 
AACCTGATCT TGGACTCCGG GATCCTCTGG CAGCTCGCCA CACGCGTTTG GCAGAATAAA 
GACCTGGGCA CGTATGGCTT TCTGGCTCTG TTTGCGTCCA CCAATGGAGG CGTCACACGT 
GTTTACCCCA ACATGGCGGC TGAATCATGG GATGAAGATC CTGAACCACT AAACTCAAAC 
TTCTACCGGC GCAGTCTGGA TAACAAAGGC TACATCTTCA GAGCGCCGAC ACGAACCTCG 
ATGGATGATC TGCTGATCTC AGAGAATGGC ACTGTGGGGA TTCTGGTTAC CACCGCCGTA 
GAGGTTGCAT TTGGGAGCAA AACCATGAAG CCTGCAGTGG TCGGCGTTAA GCTGGATTTG 
GAGGCTTGGG TCGACAAGTT CAAGATCTTG GCCAGCAACG TGTCCGACAG CAGACAGGCC 
GCACATAAGT GCGGCCCGTC CAGATCCTGT GAGATGGACT GTGAAGTGAA CAGCGATGAT 
TTGCTGTGCT ATCTGATCGA TGATGGCGGT TTCCTGGTGA TGTCCAATCA GAGAGATCAC 
TGGAAGAAGG TTGGGCTGTT TTTCGGTGAG GTCGACCCGT ACCTGATGTT CGCCCTCTAC 
AACAACTCAA TCTACTCCCG TCATCAGACC TTCCAGTATC AGTCGGCCTG CGAACCCACA 
GCCAGCAGCC ACACAGGAGC CGCACACAGA GGATTCTACG TGCCCTCCAT AGCGGACTTT 
CTCAGCCTAG CCTGGTGGAC GTCTGCAGCC GCGTGGTCTA TTTTGCAGCA GTTCTTGTAT 
GGAGTCATGT ACAGCAGCTG GTTCACCACA GACGTCTGGG CTGAGAGTGC AGACAGTAAA 
GGGAGCAGCA GCTGTGTGAC GGTTTACAGC CAGTTCTACT TCACCAACAC CACCAACTCC 
TTCAACGTCC TGCAGGACTG CGGCAACTGC TCCAGGTTGT TCCATGCCAA GCGTATAGAA 
AACACAAACC TTTTGTTTGT GGTGGCTGAG ACACTGCCCT GCAGCTCCTG CGAGATCGAG 
AAACTCCTGC CTGTCAGAAC CAAGTCTCAA GAAGAAAACC CATGTGATGT CTTAAACTCG 
GCCCGCTATA GGAAAGGACC TGACAAATGC TTCGACTACA ACTCAGAGGA GAACACATCA 
CAGTGTGGCA GCGCTCACTC TTTACACTGT CCGCTGCCAG CTCTGCTCTT GGTTCAGTTA 
TTTTTGATTT ACCTCATTCT ACGATCGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Evolution of complexity in the zebrafish synapse proteome.
Nature communications814613(2017 Mar)
Bay?s ?,Collins MO,Reig-Viader R,Gou G,Goulding D,Izquierdo A,Choudhary JS,Emes RD,Grant SG

Corticotropin-releasing hormone family evolution: five ancestral genes remain in some lineages.
Journal of molecular endocrinology57(1)73-86(2016 07)
Cardoso JC,Bergqvist CA,F?lix RC,Larhammar D

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
BMC genomics17368(2016 05)
Pasquier J,Cabau C,Nguyen T,Jouanno E,Severac D,Braasch I,Journot L,Pontarotti P,Klopp C,Postlethwait JH,Guiguen Y,Bobe J

RFX transcription factors are essential for hearing in mice.
Nature communications68549(2015 Oct)
Elkon R,Milon B,Morrison L,Shah M,Vijayakumar S,Racherla M,Leitch CC,Silipino L,Hadi S,Weiss-Gayet M,Barras E,Schmid CD,Ait-Lounis A,Barnes A,Song Y,Eisenman DJ,Eliyahu E,Frolenkov GI,Strome SE,Durand B,Zaghloul NA,Jones SM,Reith W,Hertzano R

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cacna2d2b cDNA ORF clone, Danio rerio(zebrafish)

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gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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