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epn3a cDNA ORF clone, Danio rerio(zebrafish)

Summary

Gene Symbol epn3a
Entrez Gene ID 794862
Full Name epsin 3a
Synonyms si:dkey-120c6.4
General protein information
Preferred Names

epsin 3a

Names

epsin-3
Gene Type protein-coding
Organism Danio rerio(zebrafish)

Reference Sequences (RefSeq)

mRNA and Protein(s)

mRNA Protein Name
XM_021471467.1 XP_021327142.1 epsin-3 isoform X1
XM_009299752.2 XP_009298027.1 epsin-3-like isoform X1
XM_009299752.3 XP_009298027.1 epsin-3 isoform X1
XM_005164380.2 XP_005164437.1 epsin-3-like isoform X2
XM_005164380.3 XP_005164437.1 epsin-3 isoform X2

Pathways from BioSystems


Homologies


Mus musculus (house mouse) Epn3 NP_082260.1
Gallus gallus (chicken) EPN3 XP_003642413.2
Canis lupus familiaris (dog) EPN3 XP_005624698.1
Rattus norvegicus (Norway rat) Epn3 NP_001019962.1
Pan troglodytes (chimpanzee) EPN3 XP_003315664.1
Bos taurus (cattle) EPN3 NP_001039899.1
Danio rerio (zebrafish) LOC794862 XP_005164437.1
Homo sapiens (human) EPN3 NP_060427.2
Danio rerio (zebrafish) LOC571453 XP_700135.5
Xenopus tropicalis (tropical clawed frog) epn3 NP_001005693.1

Gene Ontology

The following epn3a gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the epn3a cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
ODa08737 XM_005164380.3
Latest version!		 Danio rerio epsin 3a (epn3a), transcript variant X2, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $342.30
$489.00
ODa46946 XM_009299752.3
Latest version!		 Danio rerio epsin 3a (epn3a), transcript variant X1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $342.30
$489.00
ODa144460 XM_021471467.1
Latest version!		 Danio rerio epsin 3a (epn3a), transcript variant X1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 14-16 $342.30
$489.00
View Old Accession Versions >>
ODa46946 XM_009299752.2 Danio rerio epsin 3a (epn3a), transcript variant X1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $342.30
$489.00
ODa08737 XM_005164380.2 Danio rerio epsin 3a (epn3a), transcript variant X2, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $342.30
$489.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)


CloneID ODa08737
Clone ID Related Accession (Same CDS sequence) XM_005164380.2 , XM_005164380.3
Accession Version XM_005164380.2 Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 1914bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2016-06-23
Organism Danio rerio(Zebrafish)
Product epsin-3-like isoform X2
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007114.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 24, 2014 this sequence version replaced XM_005164380.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
ATGACGACCT CTTCGTTACG AAGGCAGGTC AAAAACATTG TAAACAACTA CACAGATGCT 
GAAATCAAGG TCAGAGAGGC AACTTCCAAT GACCCCTGGG GTCCTCCTTC CTCACTCATG 
ATGGAAATCT CTGATCTGAC CTTCAACGTG GTGGCTTTCA CCGAGGTCAT GGGCATCATC 
TGGAAGAGGC TAAATGACCA TGGCAAGAAC TGGCGGCACG TGTACAAGGC GCTTAACCTG 
CTGGAATATC TGATCAAGAC TGGGTCAGAG AGAGTGGCCC AGCAGTGCAA GGAGAACATC 
TACGCCATCC AGACCCTTCG GGACTTTCAG TACATTGACC GGGATGGACA AGACCAGGGC 
ATGAGTGTGC GGGAAAAGTC TAAGCAGCTG GTGGCTCTGC TGAGGGACGA TGAGCGGCTC 
AAGCAGGAAA GATCGCAGGC ACACAAAACC CGTGAGCGTG TTACAGGGAG CACCAGTGCC 
ATGGGTTACG GGTCTTTACC GCCACCCTAT CCGGGACGCC ACCCTAGTCA GCCCAGCATG 
CAAGCAGTTC ATGTGGACGA GTTTGCCAGG CCTAGGGACT CACCAGCCTC CTTCAACTCC 
TCGTCTTCCT CCCCTCATCT GGCCCCAGAT CTGGAGCGGG CCCGTCCTCA GACCAGTGGA 
GAGGAGGAAC TTCAGCTACA GCTGGCCTTG GCTATGAGCC GAGAGGAGAG CGAGAAGCCG 
ACTCCAGCTG TGGATATTGA TGAACAGACG CAGCTTCAGA TTGCTATGAG TCTTAGTAAG 
GAGGAGACAC ATAAGCCTAC TCCTCTTCCT CCTCCCCCTC CTCCTCCTGC TGCTGCGGTT 
ATGGAGATGG ATGAAGAGGC ACAGCTTCAG CTCGCCCTCA CTCTCAGTAA AGAGGAACAT 
CAGCAGGAGG AGAGGAGCCG TCGGGGGGAT GAGTCTCTGC TCCAGAAAGC ACTTGAGGAG 
AGCAAACGAG AAATGGAGGC CAAAGGAGGA TCTGCCATGC TGGATCTTGC AGATATTTTC 
ACTTCGGCTC CTGAACTGCC CTCTGCTGCC AGTGCTTGGG ATTCATCTGG AGGCCAAGGT 
GCTTTAGCGC AGGCTGGTCC TCTGCGGTCT GACCCATGGG ATAGTCTAGA GCCCAGCTCT 
TCAATCCGCC GGCATGGAAG CTCATGGAAT GCACCTCCTG CATCAAGTGA ATATTCCCAA 
CCCTGGGCAC ATCGACAGCA AACTTCTGAC CCATGGGATG CCCCTCAGAA TGCCTCCAGC 
CCTGTGTCCA CGAATCACAC CTGGATCTCC CCTGCACATG GGGCTGGAGT GGATCCTTTT 
TCTCCCCCCG TAGTGGACAG ACGAGGATCT ATTGGCCCTC TCAAAACTTC ACCCCCTCGT 
GCAGGAAGTC CCTCAGATGA TGATCTTTTT GATGAGGCTA TGGATGGAGG CCAGGCAAAT 
GTGAATGGCC ATGGTAGTCC TGAGCCTTTC AATTTGTCTT CCCTTGGCGA AGGTCTACCA 
AAACCAGCTC CTCGAACCTG CAGTACTCCT GAGGCATTTC TGGGTCCATC TGGAGCTTCA 
CTTGTCAACT TGGATTCTCT GATACCCCCA AATCCTACTT CCAAGAACTT CAACCCATTT 
TTATCAGGAG TGCCTGCTCC GACAACCTCT AATCCATTCC AAAATGAAGC ACCCCGCCTG 
ACGCTTAATC AGATGCGTCC CAGCTCCACG TCTCCCGCAC CCTCATCACT TCCATTCAAT 
GCGTCTCTGG CCATGGCATC CAGCCAGCAG CCCAGCTCTT TGCCCAACAC CTTCACACAG 
TCTGCACAGG TGATGCCGCT GATCCCTGGA CCCCTACCGC AACCACTGCT ACCACTGTCC 
AGCAAAACCA TGCTTGCAGA TCCAGATACA CACAATCAGA ACCCGTTCCT CTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_005164437.1
CDS430..2343
Translation

Target ORF information:

RefSeq Version XM_005164380.2
Organism Danio rerio(Zebrafish)
Definition Danio rerio epsin 3a (epn3a), transcript variant X2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 XM_005164380.2

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
ATGACGACCT CTTCGTTACG AAGGCAGGTC AAAAACATTG TAAACAACTA CACAGATGCT 
GAAATCAAGG TCAGAGAGGC AACTTCCAAT GACCCCTGGG GTCCTCCTTC CTCACTCATG 
ATGGAAATCT CTGATCTGAC CTTCAACGTG GTGGCTTTCA CCGAGGTCAT GGGCATCATC 
TGGAAGAGGC TAAATGACCA TGGCAAGAAC TGGCGGCACG TGTACAAGGC GCTTAACCTG 
CTGGAATATC TGATCAAGAC TGGGTCAGAG AGAGTGGCCC AGCAGTGCAA GGAGAACATC 
TACGCCATCC AGACCCTTCG GGACTTTCAG TACATTGACC GGGATGGACA AGACCAGGGC 
ATGAGTGTGC GGGAAAAGTC TAAGCAGCTG GTGGCTCTGC TGAGGGACGA TGAGCGGCTC 
AAGCAGGAAA GATCGCAGGC ACACAAAACC CGTGAGCGTG TTACAGGGAG CACCAGTGCC 
ATGGGTTACG GGTCTTTACC GCCACCCTAT CCGGGACGCC ACCCTAGTCA GCCCAGCATG 
CAAGCAGTTC ATGTGGACGA GTTTGCCAGG CCTAGGGACT CACCAGCCTC CTTCAACTCC 
TCGTCTTCCT CCCCTCATCT GGCCCCAGAT CTGGAGCGGG CCCGTCCTCA GACCAGTGGA 
GAGGAGGAAC TTCAGCTACA GCTGGCCTTG GCTATGAGCC GAGAGGAGAG CGAGAAGCCG 
ACTCCAGCTG TGGATATTGA TGAACAGACG CAGCTTCAGA TTGCTATGAG TCTTAGTAAG 
GAGGAGACAC ATAAGCCTAC TCCTCTTCCT CCTCCCCCTC CTCCTCCTGC TGCTGCGGTT 
ATGGAGATGG ATGAAGAGGC ACAGCTTCAG CTCGCCCTCA CTCTCAGTAA AGAGGAACAT 
CAGCAGGAGG AGAGGAGCCG TCGGGGGGAT GAGTCTCTGC TCCAGAAAGC ACTTGAGGAG 
AGCAAACGAG AAATGGAGGC CAAAGGAGGA TCTGCCATGC TGGATCTTGC AGATATTTTC 
ACTTCGGCTC CTGAACTGCC CTCTGCTGCC AGTGCTTGGG ATTCATCTGG AGGCCAAGGT 
GCTTTAGCGC AGGCTGGTCC TCTGCGGTCT GACCCATGGG ATAGTCTAGA GCCCAGCTCT 
TCAATCCGCC GGCATGGAAG CTCATGGAAT GCACCTCCTG CATCAAGTGA ATATTCCCAA 
CCCTGGGCAC ATCGACAGCA AACTTCTGAC CCATGGGATG CCCCTCAGAA TGCCTCCAGC 
CCTGTGTCCA CGAATCACAC CTGGATCTCC CCTGCACATG GGGCTGGAGT GGATCCTTTT 
TCTCCCCCCG TAGTGGACAG ACGAGGATCT ATTGGCCCTC TCAAAACTTC ACCCCCTCGT 
GCAGGAAGTC CCTCAGATGA TGATCTTTTT GATGAGGCTA TGGATGGAGG CCAGGCAAAT 
GTGAATGGCC ATGGTAGTCC TGAGCCTTTC AATTTGTCTT CCCTTGGCGA AGGTCTACCA 
AAACCAGCTC CTCGAACCTG CAGTACTCCT GAGGCATTTC TGGGTCCATC TGGAGCTTCA 
CTTGTCAACT TGGATTCTCT GATACCCCCA AATCCTACTT CCAAGAACTT CAACCCATTT 
TTATCAGGAG TGCCTGCTCC GACAACCTCT AATCCATTCC AAAATGAAGC ACCCCGCCTG 
ACGCTTAATC AGATGCGTCC CAGCTCCACG TCTCCCGCAC CCTCATCACT TCCATTCAAT 
GCGTCTCTGG CCATGGCATC CAGCCAGCAG CCCAGCTCTT TGCCCAACAC CTTCACACAG 
TCTGCACAGG TGATGCCGCT GATCCCTGGA CCCCTACCGC AACCACTGCT ACCACTGTCC 
AGCAAAACCA TGCTTGCAGA TCCAGATACA CACAATCAGA ACCCGTTCCT CTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID ODa08737
Clone ID Related Accession (Same CDS sequence) XM_005164380.2 , XM_005164380.3
Accession Version XM_005164380.3 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 1914bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2017-06-14
Organism Danio rerio(zebrafish)
Product epsin-3 isoform X2
Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007114.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005164380.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
ATGACGACCT CTTCGTTACG AAGGCAGGTC AAAAACATTG TAAACAACTA CACAGATGCT 
GAAATCAAGG TCAGAGAGGC AACTTCCAAT GACCCCTGGG GTCCTCCTTC CTCACTCATG 
ATGGAAATCT CTGATCTGAC CTTCAACGTG GTGGCTTTCA CCGAGGTCAT GGGCATCATC 
TGGAAGAGGC TAAATGACCA TGGCAAGAAC TGGCGGCACG TGTACAAGGC GCTTAACCTG 
CTGGAATATC TGATCAAGAC TGGGTCAGAG AGAGTGGCCC AGCAGTGCAA GGAGAACATC 
TACGCCATCC AGACCCTTCG GGACTTTCAG TACATTGACC GGGATGGACA AGACCAGGGC 
ATGAGTGTGC GGGAAAAGTC TAAGCAGCTG GTGGCTCTGC TGAGGGACGA TGAGCGGCTC 
AAGCAGGAAA GATCGCAGGC ACACAAAACC CGTGAGCGTG TTACAGGGAG CACCAGTGCC 
ATGGGTTACG GGTCTTTACC GCCACCCTAT CCGGGACGCC ACCCTAGTCA GCCCAGCATG 
CAAGCAGTTC ATGTGGACGA GTTTGCCAGG CCTAGGGACT CACCAGCCTC CTTCAACTCC 
TCGTCTTCCT CCCCTCATCT GGCCCCAGAT CTGGAGCGGG CCCGTCCTCA GACCAGTGGA 
GAGGAGGAAC TTCAGCTACA GCTGGCCTTG GCTATGAGCC GAGAGGAGAG CGAGAAGCCG 
ACTCCAGCTG TGGATATTGA TGAACAGACG CAGCTTCAGA TTGCTATGAG TCTTAGTAAG 
GAGGAGACAC ATAAGCCTAC TCCTCTTCCT CCTCCCCCTC CTCCTCCTGC TGCTGCGGTT 
ATGGAGATGG ATGAAGAGGC ACAGCTTCAG CTCGCCCTCA CTCTCAGTAA AGAGGAACAT 
CAGCAGGAGG AGAGGAGCCG TCGGGGGGAT GAGTCTCTGC TCCAGAAAGC ACTTGAGGAG 
AGCAAACGAG AAATGGAGGC CAAAGGAGGA TCTGCCATGC TGGATCTTGC AGATATTTTC 
ACTTCGGCTC CTGAACTGCC CTCTGCTGCC AGTGCTTGGG ATTCATCTGG AGGCCAAGGT 
GCTTTAGCGC AGGCTGGTCC TCTGCGGTCT GACCCATGGG ATAGTCTAGA GCCCAGCTCT 
TCAATCCGCC GGCATGGAAG CTCATGGAAT GCACCTCCTG CATCAAGTGA ATATTCCCAA 
CCCTGGGCAC ATCGACAGCA AACTTCTGAC CCATGGGATG CCCCTCAGAA TGCCTCCAGC 
CCTGTGTCCA CGAATCACAC CTGGATCTCC CCTGCACATG GGGCTGGAGT GGATCCTTTT 
TCTCCCCCCG TAGTGGACAG ACGAGGATCT ATTGGCCCTC TCAAAACTTC ACCCCCTCGT 
GCAGGAAGTC CCTCAGATGA TGATCTTTTT GATGAGGCTA TGGATGGAGG CCAGGCAAAT 
GTGAATGGCC ATGGTAGTCC TGAGCCTTTC AATTTGTCTT CCCTTGGCGA AGGTCTACCA 
AAACCAGCTC CTCGAACCTG CAGTACTCCT GAGGCATTTC TGGGTCCATC TGGAGCTTCA 
CTTGTCAACT TGGATTCTCT GATACCCCCA AATCCTACTT CCAAGAACTT CAACCCATTT 
TTATCAGGAG TGCCTGCTCC GACAACCTCT AATCCATTCC AAAATGAAGC ACCCCGCCTG 
ACGCTTAATC AGATGCGTCC CAGCTCCACG TCTCCCGCAC CCTCATCACT TCCATTCAAT 
GCGTCTCTGG CCATGGCATC CAGCCAGCAG CCCAGCTCTT TGCCCAACAC CTTCACACAG 
TCTGCACAGG TGATGCCGCT GATCCCTGGA CCCCTACCGC AACCACTGCT ACCACTGTCC 
AGCAAAACCA TGCTTGCAGA TCCAGATACA CACAATCAGA ACCCGTTCCT CTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_005164437.1
CDS398..2311
Translation

Target ORF information:

RefSeq Version XM_005164380.3
Organism Danio rerio(zebrafish)
Definition Danio rerio epsin 3a (epn3a), transcript variant X2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 XM_005164380.3

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
ATGACGACCT CTTCGTTACG AAGGCAGGTC AAAAACATTG TAAACAACTA CACAGATGCT 
GAAATCAAGG TCAGAGAGGC AACTTCCAAT GACCCCTGGG GTCCTCCTTC CTCACTCATG 
ATGGAAATCT CTGATCTGAC CTTCAACGTG GTGGCTTTCA CCGAGGTCAT GGGCATCATC 
TGGAAGAGGC TAAATGACCA TGGCAAGAAC TGGCGGCACG TGTACAAGGC GCTTAACCTG 
CTGGAATATC TGATCAAGAC TGGGTCAGAG AGAGTGGCCC AGCAGTGCAA GGAGAACATC 
TACGCCATCC AGACCCTTCG GGACTTTCAG TACATTGACC GGGATGGACA AGACCAGGGC 
ATGAGTGTGC GGGAAAAGTC TAAGCAGCTG GTGGCTCTGC TGAGGGACGA TGAGCGGCTC 
AAGCAGGAAA GATCGCAGGC ACACAAAACC CGTGAGCGTG TTACAGGGAG CACCAGTGCC 
ATGGGTTACG GGTCTTTACC GCCACCCTAT CCGGGACGCC ACCCTAGTCA GCCCAGCATG 
CAAGCAGTTC ATGTGGACGA GTTTGCCAGG CCTAGGGACT CACCAGCCTC CTTCAACTCC 
TCGTCTTCCT CCCCTCATCT GGCCCCAGAT CTGGAGCGGG CCCGTCCTCA GACCAGTGGA 
GAGGAGGAAC TTCAGCTACA GCTGGCCTTG GCTATGAGCC GAGAGGAGAG CGAGAAGCCG 
ACTCCAGCTG TGGATATTGA TGAACAGACG CAGCTTCAGA TTGCTATGAG TCTTAGTAAG 
GAGGAGACAC ATAAGCCTAC TCCTCTTCCT CCTCCCCCTC CTCCTCCTGC TGCTGCGGTT 
ATGGAGATGG ATGAAGAGGC ACAGCTTCAG CTCGCCCTCA CTCTCAGTAA AGAGGAACAT 
CAGCAGGAGG AGAGGAGCCG TCGGGGGGAT GAGTCTCTGC TCCAGAAAGC ACTTGAGGAG 
AGCAAACGAG AAATGGAGGC CAAAGGAGGA TCTGCCATGC TGGATCTTGC AGATATTTTC 
ACTTCGGCTC CTGAACTGCC CTCTGCTGCC AGTGCTTGGG ATTCATCTGG AGGCCAAGGT 
GCTTTAGCGC AGGCTGGTCC TCTGCGGTCT GACCCATGGG ATAGTCTAGA GCCCAGCTCT 
TCAATCCGCC GGCATGGAAG CTCATGGAAT GCACCTCCTG CATCAAGTGA ATATTCCCAA 
CCCTGGGCAC ATCGACAGCA AACTTCTGAC CCATGGGATG CCCCTCAGAA TGCCTCCAGC 
CCTGTGTCCA CGAATCACAC CTGGATCTCC CCTGCACATG GGGCTGGAGT GGATCCTTTT 
TCTCCCCCCG TAGTGGACAG ACGAGGATCT ATTGGCCCTC TCAAAACTTC ACCCCCTCGT 
GCAGGAAGTC CCTCAGATGA TGATCTTTTT GATGAGGCTA TGGATGGAGG CCAGGCAAAT 
GTGAATGGCC ATGGTAGTCC TGAGCCTTTC AATTTGTCTT CCCTTGGCGA AGGTCTACCA 
AAACCAGCTC CTCGAACCTG CAGTACTCCT GAGGCATTTC TGGGTCCATC TGGAGCTTCA 
CTTGTCAACT TGGATTCTCT GATACCCCCA AATCCTACTT CCAAGAACTT CAACCCATTT 
TTATCAGGAG TGCCTGCTCC GACAACCTCT AATCCATTCC AAAATGAAGC ACCCCGCCTG 
ACGCTTAATC AGATGCGTCC CAGCTCCACG TCTCCCGCAC CCTCATCACT TCCATTCAAT 
GCGTCTCTGG CCATGGCATC CAGCCAGCAG CCCAGCTCTT TGCCCAACAC CTTCACACAG 
TCTGCACAGG TGATGCCGCT GATCCCTGGA CCCCTACCGC AACCACTGCT ACCACTGTCC 
AGCAAAACCA TGCTTGCAGA TCCAGATACA CACAATCAGA ACCCGTTCCT CTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID ODa46946
Clone ID Related Accession (Same CDS sequence) XM_009299752.2 , XM_009299752.3
Accession Version XM_009299752.2 Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 1938bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2016-06-23
Organism Danio rerio(Zebrafish)
Product epsin-3-like isoform X1
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007114.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009299752.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
ATGACGACCT CTTCGTTACG AAGGCAGGTC AAAAACATTG TAAACAACTA CACAGATGCT 
GAAATCAAGG TCAGAGAGGC AACTTCCAAT GACCCCTGGG GTCCTCCTTC CTCACTCATG 
ATGGAAATCT CTGATCTGAC CTTCAACGTG GTGGCTTTCA CCGAGGTCAT GGGCATCATC 
TGGAAGAGGC TAAATGACCA TGGCAAGAAC TGGCGGCACG TGTACAAGGC GCTTAACCTG 
CTGGAATATC TGATCAAGAC TGGGTCAGAG AGAGTGGCCC AGCAGTGCAA GGAGAACATC 
TACGCCATCC AGACCCTTCG GGACTTTCAG TACATTGACC GGGATGGACA AGACCAGGGC 
ATGAGTGTGC GGGAAAAGTC TAAGCAGCTG GTGGCTCTGC TGAGGGACGA TGAGCGGCTC 
AAGCAGGAAA GATCGCAGGC ACACAAAACC CGTGAGCGTG TTACAGGGAG CACCAGTGCC 
ATGGGTTACG GGTCTTTACC GCCACCCTAT CCGGGACGCC ACCCTAGTCA GCCCAGCATG 
CAAGCAGTTC ATGTGGACGA GTTTGCCAGG CCTAGGGACT CACCAGCCTC CTTCAACTCC 
TCGTCTTCCT CCCCTCATCT GGCCCCAGAT CTGGAGCGGG CCCGTCCTCA GACCAGTGGA 
GAGGAGGAAC TTCAGCTACA GCTGGCCTTG GCTATGAGCC GAGAGGAGAG CGAGAAGACC 
CATGAATCTG TTCTCCCCCA GCCGACTCCA GCTGTGGATA TTGATGAACA GACGCAGCTT 
CAGATTGCTA TGAGTCTTAG TAAGGAGGAG ACACATAAGC CTACTCCTCT TCCTCCTCCC 
CCTCCTCCTC CTGCTGCTGC GGTTATGGAG ATGGATGAAG AGGCACAGCT TCAGCTCGCC 
CTCACTCTCA GTAAAGAGGA ACATCAGCAG GAGGAGAGGA GCCGTCGGGG GGATGAGTCT 
CTGCTCCAGA AAGCACTTGA GGAGAGCAAA CGAGAAATGG AGGCCAAAGG AGGATCTGCC 
ATGCTGGATC TTGCAGATAT TTTCACTTCG GCTCCTGAAC TGCCCTCTGC TGCCAGTGCT 
TGGGATTCAT CTGGAGGCCA AGGTGCTTTA GCGCAGGCTG GTCCTCTGCG GTCTGACCCA 
TGGGATAGTC TAGAGCCCAG CTCTTCAATC CGCCGGCATG GAAGCTCATG GAATGCACCT 
CCTGCATCAA GTGAATATTC CCAACCCTGG GCACATCGAC AGCAAACTTC TGACCCATGG 
GATGCCCCTC AGAATGCCTC CAGCCCTGTG TCCACGAATC ACACCTGGAT CTCCCCTGCA 
CATGGGGCTG GAGTGGATCC TTTTTCTCCC CCCGTAGTGG ACAGACGAGG ATCTATTGGC 
CCTCTCAAAA CTTCACCCCC TCGTGCAGGA AGTCCCTCAG ATGATGATCT TTTTGATGAG 
GCTATGGATG GAGGCCAGGC AAATGTGAAT GGCCATGGTA GTCCTGAGCC TTTCAATTTG 
TCTTCCCTTG GCGAAGGTCT ACCAAAACCA GCTCCTCGAA CCTGCAGTAC TCCTGAGGCA 
TTTCTGGGTC CATCTGGAGC TTCACTTGTC AACTTGGATT CTCTGATACC CCCAAATCCT 
ACTTCCAAGA ACTTCAACCC ATTTTTATCA GGAGTGCCTG CTCCGACAAC CTCTAATCCA 
TTCCAAAATG AAGCACCCCG CCTGACGCTT AATCAGATGC GTCCCAGCTC CACGTCTCCC 
GCACCCTCAT CACTTCCATT CAATGCGTCT CTGGCCATGG CATCCAGCCA GCAGCCCAGC 
TCTTTGCCCA ACACCTTCAC ACAGTCTGCA CAGGTGATGC CGCTGATCCC TGGACCCCTA 
CCGCAACCAC TGCTACCACT GTCCAGCAAA ACCATGCTTG CAGATCCAGA TACACACAAT 
CAGAACCCGT TCCTCTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_009298027.1
CDS429..2366
Translation

Target ORF information:

RefSeq Version XM_009299752.2
Organism Danio rerio(Zebrafish)
Definition Danio rerio epsin 3a (epn3a), transcript variant X1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 XM_009299752.2

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
ATGACGACCT CTTCGTTACG AAGGCAGGTC AAAAACATTG TAAACAACTA CACAGATGCT 
GAAATCAAGG TCAGAGAGGC AACTTCCAAT GACCCCTGGG GTCCTCCTTC CTCACTCATG 
ATGGAAATCT CTGATCTGAC CTTCAACGTG GTGGCTTTCA CCGAGGTCAT GGGCATCATC 
TGGAAGAGGC TAAATGACCA TGGCAAGAAC TGGCGGCACG TGTACAAGGC GCTTAACCTG 
CTGGAATATC TGATCAAGAC TGGGTCAGAG AGAGTGGCCC AGCAGTGCAA GGAGAACATC 
TACGCCATCC AGACCCTTCG GGACTTTCAG TACATTGACC GGGATGGACA AGACCAGGGC 
ATGAGTGTGC GGGAAAAGTC TAAGCAGCTG GTGGCTCTGC TGAGGGACGA TGAGCGGCTC 
AAGCAGGAAA GATCGCAGGC ACACAAAACC CGTGAGCGTG TTACAGGGAG CACCAGTGCC 
ATGGGTTACG GGTCTTTACC GCCACCCTAT CCGGGACGCC ACCCTAGTCA GCCCAGCATG 
CAAGCAGTTC ATGTGGACGA GTTTGCCAGG CCTAGGGACT CACCAGCCTC CTTCAACTCC 
TCGTCTTCCT CCCCTCATCT GGCCCCAGAT CTGGAGCGGG CCCGTCCTCA GACCAGTGGA 
GAGGAGGAAC TTCAGCTACA GCTGGCCTTG GCTATGAGCC GAGAGGAGAG CGAGAAGACC 
CATGAATCTG TTCTCCCCCA GCCGACTCCA GCTGTGGATA TTGATGAACA GACGCAGCTT 
CAGATTGCTA TGAGTCTTAG TAAGGAGGAG ACACATAAGC CTACTCCTCT TCCTCCTCCC 
CCTCCTCCTC CTGCTGCTGC GGTTATGGAG ATGGATGAAG AGGCACAGCT TCAGCTCGCC 
CTCACTCTCA GTAAAGAGGA ACATCAGCAG GAGGAGAGGA GCCGTCGGGG GGATGAGTCT 
CTGCTCCAGA AAGCACTTGA GGAGAGCAAA CGAGAAATGG AGGCCAAAGG AGGATCTGCC 
ATGCTGGATC TTGCAGATAT TTTCACTTCG GCTCCTGAAC TGCCCTCTGC TGCCAGTGCT 
TGGGATTCAT CTGGAGGCCA AGGTGCTTTA GCGCAGGCTG GTCCTCTGCG GTCTGACCCA 
TGGGATAGTC TAGAGCCCAG CTCTTCAATC CGCCGGCATG GAAGCTCATG GAATGCACCT 
CCTGCATCAA GTGAATATTC CCAACCCTGG GCACATCGAC AGCAAACTTC TGACCCATGG 
GATGCCCCTC AGAATGCCTC CAGCCCTGTG TCCACGAATC ACACCTGGAT CTCCCCTGCA 
CATGGGGCTG GAGTGGATCC TTTTTCTCCC CCCGTAGTGG ACAGACGAGG ATCTATTGGC 
CCTCTCAAAA CTTCACCCCC TCGTGCAGGA AGTCCCTCAG ATGATGATCT TTTTGATGAG 
GCTATGGATG GAGGCCAGGC AAATGTGAAT GGCCATGGTA GTCCTGAGCC TTTCAATTTG 
TCTTCCCTTG GCGAAGGTCT ACCAAAACCA GCTCCTCGAA CCTGCAGTAC TCCTGAGGCA 
TTTCTGGGTC CATCTGGAGC TTCACTTGTC AACTTGGATT CTCTGATACC CCCAAATCCT 
ACTTCCAAGA ACTTCAACCC ATTTTTATCA GGAGTGCCTG CTCCGACAAC CTCTAATCCA 
TTCCAAAATG AAGCACCCCG CCTGACGCTT AATCAGATGC GTCCCAGCTC CACGTCTCCC 
GCACCCTCAT CACTTCCATT CAATGCGTCT CTGGCCATGG CATCCAGCCA GCAGCCCAGC 
TCTTTGCCCA ACACCTTCAC ACAGTCTGCA CAGGTGATGC CGCTGATCCC TGGACCCCTA 
CCGCAACCAC TGCTACCACT GTCCAGCAAA ACCATGCTTG CAGATCCAGA TACACACAAT 
CAGAACCCGT TCCTCTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID ODa46946
Clone ID Related Accession (Same CDS sequence) XM_009299752.2 , XM_009299752.3
Accession Version XM_009299752.3 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 1938bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2017-06-14
Organism Danio rerio(zebrafish)
Product epsin-3 isoform X1
Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007114.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009299752.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
ATGACGACCT CTTCGTTACG AAGGCAGGTC AAAAACATTG TAAACAACTA CACAGATGCT 
GAAATCAAGG TCAGAGAGGC AACTTCCAAT GACCCCTGGG GTCCTCCTTC CTCACTCATG 
ATGGAAATCT CTGATCTGAC CTTCAACGTG GTGGCTTTCA CCGAGGTCAT GGGCATCATC 
TGGAAGAGGC TAAATGACCA TGGCAAGAAC TGGCGGCACG TGTACAAGGC GCTTAACCTG 
CTGGAATATC TGATCAAGAC TGGGTCAGAG AGAGTGGCCC AGCAGTGCAA GGAGAACATC 
TACGCCATCC AGACCCTTCG GGACTTTCAG TACATTGACC GGGATGGACA AGACCAGGGC 
ATGAGTGTGC GGGAAAAGTC TAAGCAGCTG GTGGCTCTGC TGAGGGACGA TGAGCGGCTC 
AAGCAGGAAA GATCGCAGGC ACACAAAACC CGTGAGCGTG TTACAGGGAG CACCAGTGCC 
ATGGGTTACG GGTCTTTACC GCCACCCTAT CCGGGACGCC ACCCTAGTCA GCCCAGCATG 
CAAGCAGTTC ATGTGGACGA GTTTGCCAGG CCTAGGGACT CACCAGCCTC CTTCAACTCC 
TCGTCTTCCT CCCCTCATCT GGCCCCAGAT CTGGAGCGGG CCCGTCCTCA GACCAGTGGA 
GAGGAGGAAC TTCAGCTACA GCTGGCCTTG GCTATGAGCC GAGAGGAGAG CGAGAAGACC 
CATGAATCTG TTCTCCCCCA GCCGACTCCA GCTGTGGATA TTGATGAACA GACGCAGCTT 
CAGATTGCTA TGAGTCTTAG TAAGGAGGAG ACACATAAGC CTACTCCTCT TCCTCCTCCC 
CCTCCTCCTC CTGCTGCTGC GGTTATGGAG ATGGATGAAG AGGCACAGCT TCAGCTCGCC 
CTCACTCTCA GTAAAGAGGA ACATCAGCAG GAGGAGAGGA GCCGTCGGGG GGATGAGTCT 
CTGCTCCAGA AAGCACTTGA GGAGAGCAAA CGAGAAATGG AGGCCAAAGG AGGATCTGCC 
ATGCTGGATC TTGCAGATAT TTTCACTTCG GCTCCTGAAC TGCCCTCTGC TGCCAGTGCT 
TGGGATTCAT CTGGAGGCCA AGGTGCTTTA GCGCAGGCTG GTCCTCTGCG GTCTGACCCA 
TGGGATAGTC TAGAGCCCAG CTCTTCAATC CGCCGGCATG GAAGCTCATG GAATGCACCT 
CCTGCATCAA GTGAATATTC CCAACCCTGG GCACATCGAC AGCAAACTTC TGACCCATGG 
GATGCCCCTC AGAATGCCTC CAGCCCTGTG TCCACGAATC ACACCTGGAT CTCCCCTGCA 
CATGGGGCTG GAGTGGATCC TTTTTCTCCC CCCGTAGTGG ACAGACGAGG ATCTATTGGC 
CCTCTCAAAA CTTCACCCCC TCGTGCAGGA AGTCCCTCAG ATGATGATCT TTTTGATGAG 
GCTATGGATG GAGGCCAGGC AAATGTGAAT GGCCATGGTA GTCCTGAGCC TTTCAATTTG 
TCTTCCCTTG GCGAAGGTCT ACCAAAACCA GCTCCTCGAA CCTGCAGTAC TCCTGAGGCA 
TTTCTGGGTC CATCTGGAGC TTCACTTGTC AACTTGGATT CTCTGATACC CCCAAATCCT 
ACTTCCAAGA ACTTCAACCC ATTTTTATCA GGAGTGCCTG CTCCGACAAC CTCTAATCCA 
TTCCAAAATG AAGCACCCCG CCTGACGCTT AATCAGATGC GTCCCAGCTC CACGTCTCCC 
GCACCCTCAT CACTTCCATT CAATGCGTCT CTGGCCATGG CATCCAGCCA GCAGCCCAGC 
TCTTTGCCCA ACACCTTCAC ACAGTCTGCA CAGGTGATGC CGCTGATCCC TGGACCCCTA 
CCGCAACCAC TGCTACCACT GTCCAGCAAA ACCATGCTTG CAGATCCAGA TACACACAAT 
CAGAACCCGT TCCTCTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_009298027.1
CDS397..2334
Translation

Target ORF information:

RefSeq Version XM_009299752.3
Organism Danio rerio(zebrafish)
Definition Danio rerio epsin 3a (epn3a), transcript variant X1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 XM_009299752.3

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
ATGACGACCT CTTCGTTACG AAGGCAGGTC AAAAACATTG TAAACAACTA CACAGATGCT 
GAAATCAAGG TCAGAGAGGC AACTTCCAAT GACCCCTGGG GTCCTCCTTC CTCACTCATG 
ATGGAAATCT CTGATCTGAC CTTCAACGTG GTGGCTTTCA CCGAGGTCAT GGGCATCATC 
TGGAAGAGGC TAAATGACCA TGGCAAGAAC TGGCGGCACG TGTACAAGGC GCTTAACCTG 
CTGGAATATC TGATCAAGAC TGGGTCAGAG AGAGTGGCCC AGCAGTGCAA GGAGAACATC 
TACGCCATCC AGACCCTTCG GGACTTTCAG TACATTGACC GGGATGGACA AGACCAGGGC 
ATGAGTGTGC GGGAAAAGTC TAAGCAGCTG GTGGCTCTGC TGAGGGACGA TGAGCGGCTC 
AAGCAGGAAA GATCGCAGGC ACACAAAACC CGTGAGCGTG TTACAGGGAG CACCAGTGCC 
ATGGGTTACG GGTCTTTACC GCCACCCTAT CCGGGACGCC ACCCTAGTCA GCCCAGCATG 
CAAGCAGTTC ATGTGGACGA GTTTGCCAGG CCTAGGGACT CACCAGCCTC CTTCAACTCC 
TCGTCTTCCT CCCCTCATCT GGCCCCAGAT CTGGAGCGGG CCCGTCCTCA GACCAGTGGA 
GAGGAGGAAC TTCAGCTACA GCTGGCCTTG GCTATGAGCC GAGAGGAGAG CGAGAAGACC 
CATGAATCTG TTCTCCCCCA GCCGACTCCA GCTGTGGATA TTGATGAACA GACGCAGCTT 
CAGATTGCTA TGAGTCTTAG TAAGGAGGAG ACACATAAGC CTACTCCTCT TCCTCCTCCC 
CCTCCTCCTC CTGCTGCTGC GGTTATGGAG ATGGATGAAG AGGCACAGCT TCAGCTCGCC 
CTCACTCTCA GTAAAGAGGA ACATCAGCAG GAGGAGAGGA GCCGTCGGGG GGATGAGTCT 
CTGCTCCAGA AAGCACTTGA GGAGAGCAAA CGAGAAATGG AGGCCAAAGG AGGATCTGCC 
ATGCTGGATC TTGCAGATAT TTTCACTTCG GCTCCTGAAC TGCCCTCTGC TGCCAGTGCT 
TGGGATTCAT CTGGAGGCCA AGGTGCTTTA GCGCAGGCTG GTCCTCTGCG GTCTGACCCA 
TGGGATAGTC TAGAGCCCAG CTCTTCAATC CGCCGGCATG GAAGCTCATG GAATGCACCT 
CCTGCATCAA GTGAATATTC CCAACCCTGG GCACATCGAC AGCAAACTTC TGACCCATGG 
GATGCCCCTC AGAATGCCTC CAGCCCTGTG TCCACGAATC ACACCTGGAT CTCCCCTGCA 
CATGGGGCTG GAGTGGATCC TTTTTCTCCC CCCGTAGTGG ACAGACGAGG ATCTATTGGC 
CCTCTCAAAA CTTCACCCCC TCGTGCAGGA AGTCCCTCAG ATGATGATCT TTTTGATGAG 
GCTATGGATG GAGGCCAGGC AAATGTGAAT GGCCATGGTA GTCCTGAGCC TTTCAATTTG 
TCTTCCCTTG GCGAAGGTCT ACCAAAACCA GCTCCTCGAA CCTGCAGTAC TCCTGAGGCA 
TTTCTGGGTC CATCTGGAGC TTCACTTGTC AACTTGGATT CTCTGATACC CCCAAATCCT 
ACTTCCAAGA ACTTCAACCC ATTTTTATCA GGAGTGCCTG CTCCGACAAC CTCTAATCCA 
TTCCAAAATG AAGCACCCCG CCTGACGCTT AATCAGATGC GTCCCAGCTC CACGTCTCCC 
GCACCCTCAT CACTTCCATT CAATGCGTCT CTGGCCATGG CATCCAGCCA GCAGCCCAGC 
TCTTTGCCCA ACACCTTCAC ACAGTCTGCA CAGGTGATGC CGCTGATCCC TGGACCCCTA 
CCGCAACCAC TGCTACCACT GTCCAGCAAA ACCATGCTTG CAGATCCAGA TACACACAAT 
CAGAACCCGT TCCTCTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID ODa144460
Clone ID Related Accession (Same CDS sequence) XM_021471467.1
Accession Version XM_021471467.1 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 1938bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2017-06-14
Organism Danio rerio(zebrafish)
Product epsin-3 isoform X1
Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_018394539.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
ATGACGACCT CTTCGTTACG AAGGCAGGTC AAAAACATTG TAAACAACTA CACAGATGCT 
GAAATCAAGG TCAGAGAGGC AACTTCCAAT GACCCCTGGG GTCCTCCTTC CTCACTCATG 
ATGGAAATCT CTGATCTGAC CTTCAACGTG GTGGCTTTCA CCGAGGTCAT GGGCATCATC 
TGGAAGAGGC TAAATGACCA TGGCAAGAAC TGGCGGCACG TGTACAAGGC GCTTAACCTG 
CTGGAATATC TGATCAAGAC TGGGTCAGAG AGAGTGGCCC AGCAGTGCAA GGAGAACATC 
TACGCCATCC AGACCCTTCG GGACTTTCAG TACATTGACC GGGATGGACA AGACCAGGGC 
ATGAGTGTGC GGGAAAAGTC TAAGCAGCTG GTGGCTCTGC TGAGGGACGA TGAGCGGCTC 
AAGCAGGAAA GATCGCAGGC ACACAAAACC CGTGAGCGTG TTACAGGGAG CACCAGTGCC 
ATGGGTTACG GGTCTTTACC GCCACCCTAT CCGGGACGCC ACCCTAGTCA GCCCAGCATG 
CAAGCAGTTC ATGTGGACGA GTTTGCCAGG CCTAGGGACT CACCAGCCTC CTTCAACTCC 
TCGTCTTCCT CCCCTCATCT GGCCCCAGAT CTGGAGCGGG CCCGTCCTCA GACCAGTGGA 
GAGGAGGAAC TTCAGCTACA GCTGGCCTTG GCTATGAGCC GGGAGGAGAG TGAGAAGACC 
CATGAATCTG TTCTCCCCCA GCCGACTCCA GCTGTGGATA TTGATGAACA GACGCAGCTT 
CAGATTGCTA TGAGTCTTAG TAAGGAGGAG ACACATAAGC CTACTCCTCT TCCTCCTCCC 
CCTCCTCCTC CTGCTGCTGC GGTTATGGAG ATGGATGAAG AGGCACAGCT TCAGCTCGCC 
CTCACTCTCA GTAAAGAGGA ACATCAGCAG GAGGAGAGAA GCCGTCGGGG GGATGAGTCT 
CTGCTCCAGA AAGCACTTGA GGAGAGCAAA CGAGAAATGG AGGCCAAAGG AGGATCTGCC 
ATGCTGGATC TTGCAGATAT TTTCACTTCG GCTCCTGAAC TGCCCTCTGC TGCCAGTGCT 
TGGGATTCAT CTGGAGGCCA AGGTGCTTTA GCGCAGGCTG GTCCTCTGCG GTCTGACCCA 
TGGGATAGTC TAGAGCCCAG CTCTTCAATC CGCCGGCATG GAAGCTCATG GAATGCACCT 
CCTGCATCAA GTGAATATTC CCAACCCTGG GCACATCGAC AGCAAACTTC TGACCCATGG 
GATGCCCCTC AGAATGCCTC CAGCCCTGTG TCCACGAATC ACACCTGGAT CTCCCCTGCA 
CATGGGGCTG GAGTGGATCC TTTTTCTCCC CCCGTAGTGG ACAGACGAGG ATCTATTGGC 
CCTCTCAAAA CTTCACCCCC TCGTGCAGGA AGTCCCTCAG ATGATGATCT TTTTGATGAG 
GCTATGGATG GAGGCCAGGC AAATGTGAAT GGCCATGGTA GTCCTGAGCC TTTCAATTTG 
TCTTCCCTTG GCGAAGGTCT ACCAAAACCA GCTCCTCGAA CCTGCAGTAC TCCTGAGGCA 
TTTCTGGGTC CATCTGGAGC TTCACTTGTC AACTTGGATT CTCTGATACC CCCAAATCCT 
ACTTCCAAGA ACTTCAACCC ATTTTTATCA GGAGTGCCTG CTCCGACAAC CTCTAATCCA 
TTCCAAAATG AAGCACCCCG CCTGACGCTT AATCAGATGC GTCCCAGCTC CACGTCTCCC 
GCACCCTCAT CACTTCCATT CAATGCGTCT CTGGCCATGG CATCCAGCCA GCAGCCCAGC 
TCTTTGCCCA ACACCTTCAC ACAGTCTGCA CAGGTGATGC CGCTGATCCC TGGACCCCTA 
CCGCAACCAC TGCTACCACT GTCCAGCAAA ACCATGCTTG CAGATCCAGA TACACACAAT 
CAGAACCCGT TCCTCTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_021327142.1
CDS397..2334
Translation

Target ORF information:

RefSeq Version XM_021471467.1
Organism Danio rerio(zebrafish)
Definition Danio rerio epsin 3a (epn3a), transcript variant X1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 XM_021471467.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
ATGACGACCT CTTCGTTACG AAGGCAGGTC AAAAACATTG TAAACAACTA CACAGATGCT 
GAAATCAAGG TCAGAGAGGC AACTTCCAAT GACCCCTGGG GTCCTCCTTC CTCACTCATG 
ATGGAAATCT CTGATCTGAC CTTCAACGTG GTGGCTTTCA CCGAGGTCAT GGGCATCATC 
TGGAAGAGGC TAAATGACCA TGGCAAGAAC TGGCGGCACG TGTACAAGGC GCTTAACCTG 
CTGGAATATC TGATCAAGAC TGGGTCAGAG AGAGTGGCCC AGCAGTGCAA GGAGAACATC 
TACGCCATCC AGACCCTTCG GGACTTTCAG TACATTGACC GGGATGGACA AGACCAGGGC 
ATGAGTGTGC GGGAAAAGTC TAAGCAGCTG GTGGCTCTGC TGAGGGACGA TGAGCGGCTC 
AAGCAGGAAA GATCGCAGGC ACACAAAACC CGTGAGCGTG TTACAGGGAG CACCAGTGCC 
ATGGGTTACG GGTCTTTACC GCCACCCTAT CCGGGACGCC ACCCTAGTCA GCCCAGCATG 
CAAGCAGTTC ATGTGGACGA GTTTGCCAGG CCTAGGGACT CACCAGCCTC CTTCAACTCC 
TCGTCTTCCT CCCCTCATCT GGCCCCAGAT CTGGAGCGGG CCCGTCCTCA GACCAGTGGA 
GAGGAGGAAC TTCAGCTACA GCTGGCCTTG GCTATGAGCC GGGAGGAGAG TGAGAAGACC 
CATGAATCTG TTCTCCCCCA GCCGACTCCA GCTGTGGATA TTGATGAACA GACGCAGCTT 
CAGATTGCTA TGAGTCTTAG TAAGGAGGAG ACACATAAGC CTACTCCTCT TCCTCCTCCC 
CCTCCTCCTC CTGCTGCTGC GGTTATGGAG ATGGATGAAG AGGCACAGCT TCAGCTCGCC 
CTCACTCTCA GTAAAGAGGA ACATCAGCAG GAGGAGAGAA GCCGTCGGGG GGATGAGTCT 
CTGCTCCAGA AAGCACTTGA GGAGAGCAAA CGAGAAATGG AGGCCAAAGG AGGATCTGCC 
ATGCTGGATC TTGCAGATAT TTTCACTTCG GCTCCTGAAC TGCCCTCTGC TGCCAGTGCT 
TGGGATTCAT CTGGAGGCCA AGGTGCTTTA GCGCAGGCTG GTCCTCTGCG GTCTGACCCA 
TGGGATAGTC TAGAGCCCAG CTCTTCAATC CGCCGGCATG GAAGCTCATG GAATGCACCT 
CCTGCATCAA GTGAATATTC CCAACCCTGG GCACATCGAC AGCAAACTTC TGACCCATGG 
GATGCCCCTC AGAATGCCTC CAGCCCTGTG TCCACGAATC ACACCTGGAT CTCCCCTGCA 
CATGGGGCTG GAGTGGATCC TTTTTCTCCC CCCGTAGTGG ACAGACGAGG ATCTATTGGC 
CCTCTCAAAA CTTCACCCCC TCGTGCAGGA AGTCCCTCAG ATGATGATCT TTTTGATGAG 
GCTATGGATG GAGGCCAGGC AAATGTGAAT GGCCATGGTA GTCCTGAGCC TTTCAATTTG 
TCTTCCCTTG GCGAAGGTCT ACCAAAACCA GCTCCTCGAA CCTGCAGTAC TCCTGAGGCA 
TTTCTGGGTC CATCTGGAGC TTCACTTGTC AACTTGGATT CTCTGATACC CCCAAATCCT 
ACTTCCAAGA ACTTCAACCC ATTTTTATCA GGAGTGCCTG CTCCGACAAC CTCTAATCCA 
TTCCAAAATG AAGCACCCCG CCTGACGCTT AATCAGATGC GTCCCAGCTC CACGTCTCCC 
GCACCCTCAT CACTTCCATT CAATGCGTCT CTGGCCATGG CATCCAGCCA GCAGCCCAGC 
TCTTTGCCCA ACACCTTCAC ACAGTCTGCA CAGGTGATGC CGCTGATCCC TGGACCCCTA 
CCGCAACCAC TGCTACCACT GTCCAGCAAA ACCATGCTTG CAGATCCAGA TACACACAAT 
CAGAACCCGT TCCTCTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

PubMed