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Ubr3 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Summary

Gene Symbol Ubr3
Entrez Gene ID 31713
Full Name Ubr3 ubiquitin ligase
Synonyms CG1530,CG1531,CG42593,Dmel\CG42593,Dmel_CG1530,Dmel_CG1531,ubr3
General protein information
Preferred Names

Ubr3 ubiquitin ligase

Names

CG42593 gene product from transcript CG42593-RA
CG42593-PA
CG42593-PB
CG42593-PC
Ubr3-PA
Ubr3-PB
Ubr3-PC
Gene Type protein-coding
Organism Drosophila melanogaster(fruit fly)
Genome

X

7C8-7C9

Reference Sequences (RefSeq)

mRNA and Protein(s)

mRNA Protein Name
NM_132200.4 NP_572428.3 Ubr3 ubiquitin ligase, isoform A
NM_001272388.1 NP_001259317.1 Ubr3 ubiquitin ligase, isoform B
NM_001272389.1 NP_001259318.1 Ubr3 ubiquitin ligase, isoform C

Pathways from BioSystems


Homologies


Bos taurus (cattle) UBR3 XP_005202447.1
Mus musculus (house mouse) Ubr3 NP_808451.2
Drosophila melanogaster (fruit fly) CG42593 NP_001259318.1
UBR3 XP_001099162.2
Anopheles gambiae (African malaria mosquito) AgaP_AGAP000384 XP_310723.5
Homo sapiens (human) UBR3 NP_742067.3
Pan troglodytes (chimpanzee) UBR3 XP_003309461.1
Canis lupus familiaris (dog) UBR3 XP_005640460.1
Rattus norvegicus (Norway rat) Ubr3 XP_006234401.1
Gallus gallus (chicken) ZNF650 XP_004942807.1
Danio rerio (zebrafish) ubr3 XP_001919629.4
Xenopus tropicalis (tropical clawed frog) ubr3 XP_002934668.2

Gene Ontology


Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Ubr3 negatively regulates the mono-ubiquitination of non-muscle Myosin II, a protein associated with hearing loss in humans.
Title: The E3 ligase Ubr3 regulates Usher syndrome and MYH9 disorder proteins in the auditory organs of Drosophila and mammals.

We identified Ubr3, an E3 ubiquitin ligase, as a novel, positive regulator of Hh signaling in Drosophila and vertebrates. Hh signaling regulates the Ubr3-mediated poly-ubiquitination and degradation of Cos2, a central component of Hh signaling. In developing Drosophila eye discs, loss of ubr3 leads to a delayed differentiation of photoreceptors and a reduction in Hh signaling.
Title: Ubr3, a Novel Modulator of Hh Signaling Affects the Degradation of Costal-2 and Kif7 through Poly-ubiquitination.

Ubr3 interacts with DIAP1 and positively regulates DIAP1 activity, possibly by maintaining its active conformation in the apoptosis pathway.
Title: Ubr3 E3 ligase regulates apoptosis by controlling the activity of DIAP1 in Drosophila.

The following Ubr3 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Ubr3 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OFa01058 NM_132200.4
Latest version!		 Drosophila melanogaster Ubr3 ubiquitin ligase (Ubr3), transcript variant A, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 30 $1161.30
$1659.00
OFa03020 NM_001272388.1
Latest version!		 Drosophila melanogaster Ubr3 ubiquitin ligase (Ubr3), transcript variant B, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 30 $1161.30
$1659.00
OFa03021 NM_001272389.1
Latest version!		 Drosophila melanogaster Ubr3 ubiquitin ligase (Ubr3), transcript variant C, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 30 $1161.30
$1659.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone 30% OFF
2-4 Clone 40% OFF
5 or more Clone 50% OFF
All Other ORF Clones
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)


CloneID OFa01058
Clone ID Related Accession (Same CDS sequence) NM_132200.4
Accession Version NM_132200.4 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 6660bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2019-04-24
Organism Drosophila melanogaster(fruit fly)
Product Ubr3 ubiquitin ligase, isoform A
Comment Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). On Jan 16, 2013 this sequence version replaced NM_132200.3. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END## 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
3721
3781
3841
3901
3961
4021
4081
4141
4201
4261
4321
4381
4441
4501
4561
4621
4681
4741
4801
4861
4921
4981
5041
5101
5161
5221
5281
5341
5401
5461
5521
5581
5641
5701
5761
5821
5881
5941
6001
6061
6121
6181
6241
6301
6361
6421
6481
6541
6601
ATGGACGAGG ATGACAACCT GTCCAATGCC GACATAAGCA TAGATGACGA GGAGGTGGTG 
CGGGAGCAGA CCCACCATCC GCCCATGCAA GAGGATCAGG AGCTGGATAA CGAAGATGGG 
TCATCCGACG TGGATCTGTC TTCGGTGTAC GTCGTGCCAC CCATAGTCTC GGCACCATCG 
CCCGCATCAG TGCGCATCGC TGGTGGATCC ACAATTGGTG GCGGAGGGGT CGCAGCTGGA 
GCAGTTCCAA CAACCACCGA TAACTCGCAT TCCCCCTTCC ACGATTGGGA TTCCAGTGTG 
GCGGCCGCTG CTGCTCCACC GCCGCCACCG CCCAGGACAG GACCAACTAC AACTACAAGC 
TCCGGCACAG CCGCGGAATC GGGAGCCGCG TCCGCCTTAT CCGACGATGC CGGCGCCAAG 
CCAAGTTTCT TCTTTGGCGC CTCCTCGTTC AGTTTGCGCA GCCGCAAGGA GGTGGCCGCC 
CTGATCAACA CGGAATGCTG CCGTGGCAGC CCCACACCCG ATCTCGACTC CATAATGGAT 
ACCCTATTCA ATCCGGGGAC GCCCATCGAC AATCTGGACA ACATCGAGTG GATACGATGG 
CTCATTGCCG GCGGACGGAC GCCGCAGGAG TTTGTTAAAA TTGTGCGCAG CTATGACAAC 
CACGCCAAAT GCGGTCTGGT ATGGGTACCG CATGTGGTGG CCTACAGGTG CCGCACTTGC 
GGCATATCGC CCTGCATGTC CATATGCCGG GACTGCTTCA AGAAGGGCAA CCACACGAAC 
CACGACTTCA ATATGTTCCT GTCACAGGCG GGCGGTGCCT GCGATTGTGG TGACACCTCG 
GTCATGAAGG CGGAGGGTTT TTGCAGTGAT CATGGCATCA ATAATCGGGT GAATCGCGAT 
CCGGTGCCAA ACAATCTTTT GGCAGTGGCC GAGGCCATAA TGCCCAAGCT GCTCTTCCGC 
CTGCTGCAGC ACTTCCGTGA GCACAGCGAC ACCCCACTGG AGGTGCAAGC GATAACGTCG 
TACTCCTGCG AGGAGTTCGC CAACATGCTG ATCGATCTGA ACAACATGGG CGAAATAATG 
CGCAAAGTGA TGACGCGCAC GCTGATAAAT CCGGAGGTGT ATGCCTTCTT CATGGAAGCA 
CCGTGCCAGG ACACCCGGAA TGGACGCTTC TTGAAGGCGA ACCGAGAGAA GTACGAGGAT 
GCCGTCAATA GATTCCCAAA CCCAGAGCCA CCCGATGAGT ACAGAGATCT GCCCGCGCTG 
GGCGACAAAT TGGTCCACAC CACGCTACTC GAAGAGTTCA TCTTCTGGAC ATTTAAGTTC 
GAGTTTCCAC AGACTCTGGT CTGCTTTCTG CTGAACATGC TGCCTGATCA GGATTACAAG 
GAACACCTCA CCCGCACATT TGTGATGCAC TACAGCCGCA TACCGTCCGT GTTGGAAATG 
TCCCGTGATC CGGACACGCT CAGCAACCGG GTGGTCCACA TGAGCGTCCA ACTCTTCTCT 
AACGAAAGTC TGGCCCTCAA GATGGTCAAC GAGCTGTCGC TGCTGCACGT AATGATTATT 
AGTCTGAAGC TAATGATGTC CAAGATACTC ATACAGAATA CATTGCATGA TCCCAACAAG 
AATTTTCATT TCGTCATCGA CTGCACGCGT CAGGTGATGA AGGACCACTG CTACTGGCCA 
CTAGTCTCGG ACTTTAACAA CGTCCTTTCA CACGAATCGG TGGCCCTGGT CTTTCTGCGG 
GACGACAATC TCATCGATAT GTGGTTCCAG TTTCTCCAGA TGCTCCAGGG CATGAATGTC 
AATGTGCGCG AGACGGCTTC TCATGTGGAA TTCGAGCCAA ATAGCTACTA TGCGGCGTTC 
TCCTGTGAGC TTGAGGCCAG TGCCTATCCC ATGTGGTCGA TTATCTCGCA TCTGCAGGAT 
GGCACACATG CCCATCTGGC AAAGAAGATC ATCAACTACT GTGTGACGAC GCTGCACGAG 
TGGCTAGACT CTATTTACTT TATGGAGGCG CGTCTATCTA TGGAGGAGAT GATGCAGGCT 
TCGTTCCACT TTCCCCTGCA TCGTTATCTG GCTGCCTTTG TTTGCCAGGC GGTAACTAAA 
ATGGGAATCA GTCTGAACGA TGTGCTGCCC TCACGCCCCT ATCTGCTGCC CCTGCTGATG 
ATCCATCCAC TTCGCGTCCA GAGTTTTTTC TACGAGATCC TGGCCGGAAA ATGGGTGCGC 
AATGGTCTCC AAATCAAGGG ACAAGCGATG ACCTATATCC AGGCGAACTT CTGCAACTCG 
ATGGCCGACA TGGACCTGTT CTTCCTGCAA ATCTGTGCCA CCAACCTGCC GCAGTACTTC 
TTTCTGCAGA ACACCATCGA GTTGTTCGAC GTAGGTCAAT GGCTGGAGAC AGCTCCCTTG 
AAGCAGCCTC AAAAGGCGGA GCAATCCTCG ATGCTGGAGG GATTCCTAAC CTTCTTGGCC 
ACCCTGGTGA CGAGTCGCAC TAATCTGGGA AACGATGAAG CCACCCAATG CATTATTGAG 
ATCAGTGCCT TGTTGGCTAC CGAGAACAAG ACGCATTCGC AGCTCCTTGA ACTAATGCCG 
GAGCGGTCGG GCAATGTGCA TACCAAAAAC TTTGAGACCT TCCTTAAGAA GCTATCGGTT 
TACAAGGCTC CGTCGAGTGG GAGCGAGAAC CTGGAGCAGG GTCTCTTCAC GCCCATCGAC 
GAGGTGTGGG AGAAGCACTA CGATCCGCTG CACGTCCTTT TGCGAGCAGT TCATCGTCGA 
GACTTTCAAT CATCGCTGGA CCGCTTTACA AACTATGTGA AGAGCAAGGA TAAGATGCCG 
GCAAGTGGTA ACCTATGGCC ACCCTTCCGA TTGCCTCATG CTCTGCCCGC AACGAGCTCC 
TTCAGTGATC CCTGCAAGAT TTTGAACTCG CGCATCTTTC ACTCCACCAT CCTGTCGATA 
TTTTTCCGTG CAGTTCACAC ACGTGATGTT TCCGAGCATC TGCTGGCTCT GGCTGTGTTC 
CTCTTAGAGA TTGCTGTGGA AACAAGTGAT GATGTTGGCA GTGGAACTGG AGATCGGGCC 
CCTCCTGCGC TGGCTGCTGT GGTGGAGTCA TCTAGCGGGC CGGGCTATCA TGGCTACGGC 
ACGGGACGGC ATGAGCCGCC CAAGCTTTTC CACTGCTATC CCACGGACAA TCTAAGCTGC 
AATCTGCGCC ACGTGGTCAA GAAAGTGTCG CTGAAGTCAC GCGATCCGCA AGTGATCACC 
TCCAGCTATC GGTCGAACCC ATTTTACTCA GATCTGGACT TTGAGGTTGA AGCGGATCCA 
GAGCAATCGA TGCGCATGAT TGGTCAAGGG GACCCCGAAG GAGATGAGGC AACGGGTGGA 
GCTGGACTGG GAATGGGAGC TCATAGCAGA AGGAATGTCT CTCAGGCGCT GGTGCCGATG 
AGAGTGCCCG GCATGGAGGT CGCCCTACCA CCGGATCTTT CAGTAGTTGC CGAAACCGGT 
GTGGTTATCC GCCAGGATAG CAACGAAGAT GATCTACTGA GAGAAGGCAA TCATGCCATG 
GATATGAGTC CACCGGCTGC TCTCGATTTC CACTTTCCGC TGCAGCAGAT CACTTTGCCG 
GAGAGCGGAA TGGAGGTGGC TATTCGGAGA GATCTTCTGC TAGCCGAAAC GAATAATATG 
GGCGCAATTG CAGGTGGAGC TGGAGGTGGC GCCAATGCAT CGGCCACACC ACCAGCAGGG 
GCTTCCAATG AGATGTTCTC GCCTACCACA CCCACAGGCA GCGGTATGCT CCTGCCTTTC 
CAGCGGGTTC AGCCTGTAGC CGTACCCAGT AGCGGTAATA TGGATATTGT ACCATCAAAT 
GCCATGGGTG CCGGCGGAAG TTTCACATCC GGCGTAAGTG GTAGCGGCAC TGGACGTAGA 
ATGAACTACG AAACGGGTGG AGCACGTAAG CGATCCGTGG ACATTGCCAT CGGTGGCAGC 
AATAAGGATG AGCTGCATTT GGACGAAAGC ATTTTGTCCC TGCTACTAAA GCTTCATTCT 
CAGCTCTCAG GCACACTAGA TAGCTTCTCG TTGAGCGACG GTGAAGATCA ATCATCTGAC 
GACGATTCGA CCATGGATGT GGACTGTAAC GAAGCCAGCA CCTCCATGGC AGCAGCGGAG 
AGCACTGCCC TGGCTGAGAG AGGACGCAGT AAGCGCAACT ACAAGAACAT TCATGTATCC 
TCCTCGCGCA TCGGTGACGG ACCATTCTTT ATTGGTAATC TGCTGCGAAA GATCGCCAAG 
CAGGATGAGC AGTGCGCCCA GAGCATCGAC GACATCCGTG CCAGACTCTG GCCGAATCAG 
CGTGAAAAAC AAGCAGAGGC GAAAGCACGC GAGGCCAAGG AGAAGGAGGA GCGCCGCAAA 
AAAGCACGTG AGCGCCAGCA GAAGATGATG CAAGACTTCG CCAACAAGCA GAAGCTCTTC 
ATGCAATCCG CTGCAGCGTC ATCCAGTGGC ATGGGCTACG GACCGGAGGA CGAGGACGAT 
GAGGAGTTGT ACGAGGAGCA GCCGCGTGAG AAAGAGTACG ATTGCATAAT CTGCAATTGC 
ACTACGCCCA GTACCGAATC GAATCCTATC GGCTTGGTCG TTCTGGTCGA GTCCAGCGGC 
ATTGTTGGTC ATCGGCGGCG CATCGCCGAG CGATTGCCGC TGCCGATAAA CGCGGAGGAT 
GAGTCCCGAC TGGCGCACAC CACTCGACTG GCGGCTGAAT TCAACAGGCG CACGGAACTG 
CTCAGTCTGA AGTTTGGCGA TGAGTCCTGG TATCTGTCCA ATAATATGGC CTACGACAAC 
GGTGTACATG TGCAGTCGTG TGGCCACCAC GTTCATCTCA GTTGCCTAGA GGCATACCTT 
AAGACGCTGT ACACCACCCA ACGTCAGCCA GTTCAGGATC GTGGCGAGTT CTACTGTCCC 
GTGTGCCGAC AGCTCTCAAA CTCGGTGCTG CCGCTGAGTC CACAACTGGA CAGACCCACG 
CACCTGGTCC GCTCTGGTAA TCAGCCATTC GAACGTCTGG TCGCCGATCT AACAGATCTG 
ATTAAAGAGA ACGAAACGAT ACCGCAGCCA ACAAAACTGA CAGAGGCCAT GGGCCATGCC 
ATGGAGGTGA TGACAAACAT TGCCCAGCGC AAGGTTAAAT GCTCCTCGAT AACGTTCCGC 
AAGCTGTTCA TTTTCGTTAC CTCGATTGCG CGTACAAACC TGGAGGCGGA GATCATCCAG 
CGGGGCGGAT CACTATGTAC AGCGAATGCC ACGCGGTACA AGCCAAAGCG GGAATGCATC 
GTACCGCTAC TGCACGTGCT ATCGGTACAT GTGCGTGTCT TGGTTGAATG GCCGCTGTGG 
AGCAGCTGGG CCTCGTTAGC TGGCCTGCCC GTTACCGCTA CGGAACCGTT GCCGGCGCAC 
TGCCTCGAAT TGATTCCCAG CATATTAGCG GATCCCATTG CTCTGCTGCT CAAGTTTATC 
CTGCTGGCGC CACTGCAACT CGATCAGGAT TACTTTACGT GCATGGTGAA AGTCATGTAC 
AATCTGCTGT ACTATCAAAT TGTGGTTCAG CTGTGCGTTA CTCTCACCGA TTTAGAGTGC 
GATCACATTG TCAAAGTTTA CGGCAGCACT AGCGTTGGCA GTGACAACTC TGCTGCGGAA 
TCCCAACAGC AGGAGTCTGC CGCCGGGACA ACGAACAACC GTCGCCGTGC TGGACAACAG 
CAGCAGAGCT CCTCGCAGCT AGGAAAGGCC ATGGCCCTGG TGCTGAGCCA GACAAATGAC 
CTAGTTCACT TGCGACGCGA CTGCATACCC AGTACCAGCA GCTCGGCGGC AGCGTCGGCG 
GCTGGTTCCT CCTCCACAAC GTCCACAAAC CATGGAGCTA GCGCAGCCAC AGCCTCCTCT 
GCCACGACGA TCGAGGTTAA TCTGAAGTCC ATGGAATTAC AGCTGCAGGC GCTATGCTTG 
CCGTTTTTGC GAGTAGCTGC CCTGCTGCGC CAGCATCTCT ATCGCCATGA GATGCCGGAG 
ATCTCGGCAC CGGGACTGGA ATTTGTGCGC CTTGTTTACT ATTTGGAACT CGTAACAGAT 
TCTATGGACT GGGATTGCTT TAATGCCAGC AAGGGGCTAT GCTTTATTCC CGGCACTGAG 
ACAACGCTGC CCCAGTTCTG GTGCCAGCAG TTGATGGAGG TGCGTCCGCC GGCCGATACG 
GTCAGAGAAT TGGTGCTCAT CAATCAGCAT TCGTTGTGGC AGCAGCCGCG ACTTCTTGAA 
TTGCCGCGCG AGTACGAACG CTTGTTCACG TACTACCATG AGCGGCCGTG CCTCAATTGC 
TATAAAGTGC CCAAGGAGAG CTCCATCTGC CTGCTGTGCG GGACGATCGT ATGCCTCAAG 
CAGAACTGCT GCGCGGAGAA TGACTGCTGC GAGGCTGTTC GCCATACGCT ATCCTGTGGC 
GGTGGCATAG GCATCTTTTT GGTGGTCACC TCCACGTACA TCATTGTTAT CCGCGGACGA 
CGCGCCTGCC TTTGGGGCTC ACTCTATCTG GACGACTTCG ATGAGGAGGA TCGCGATCTC 
AAGCGCGGCA AACCTTTGTA TTTGAGCAAA GACCGCTTCA ATCTCCTGGA GTCGCAGTGG 
CTGTCCCATA AGTTTGCTCA TACAAAACAC ACATGGGTGT TTCACCGGGA TCTCTTGTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_572428.3
CDS1074..7733
Translation

Target ORF information:

RefSeq Version NM_132200.4
Organism Drosophila melanogaster(fruit fly)
Definition Drosophila melanogaster Ubr3 ubiquitin ligase (Ubr3), transcript variant A, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 NM_132200.4

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
3721
3781
3841
3901
3961
4021
4081
4141
4201
4261
4321
4381
4441
4501
4561
4621
4681
4741
4801
4861
4921
4981
5041
5101
5161
5221
5281
5341
5401
5461
5521
5581
5641
5701
5761
5821
5881
5941
6001
6061
6121
6181
6241
6301
6361
6421
6481
6541
6601
ATGGACGAGG ATGACAACCT GTCCAATGCC GACATAAGCA TAGATGACGA GGAGGTGGTG 
CGGGAGCAGA CCCACCATCC GCCCATGCAA GAGGATCAGG AGCTGGATAA CGAAGATGGG 
TCATCCGACG TGGATCTGTC TTCGGTGTAC GTCGTGCCAC CCATAGTCTC GGCACCATCG 
CCCGCATCAG TGCGCATCGC TGGTGGATCC ACAATTGGTG GCGGAGGGGT CGCAGCTGGA 
GCAGTTCCAA CAACCACCGA TAACTCGCAT TCCCCCTTCC ACGATTGGGA TTCCAGTGTG 
GCGGCCGCTG CTGCTCCACC GCCGCCACCG CCCAGGACAG GACCAACTAC AACTACAAGC 
TCCGGCACAG CCGCGGAATC GGGAGCCGCG TCCGCCTTAT CCGACGATGC CGGCGCCAAG 
CCAAGTTTCT TCTTTGGCGC CTCCTCGTTC AGTTTGCGCA GCCGCAAGGA GGTGGCCGCC 
CTGATCAACA CGGAATGCTG CCGTGGCAGC CCCACACCCG ATCTCGACTC CATAATGGAT 
ACCCTATTCA ATCCGGGGAC GCCCATCGAC AATCTGGACA ACATCGAGTG GATACGATGG 
CTCATTGCCG GCGGACGGAC GCCGCAGGAG TTTGTTAAAA TTGTGCGCAG CTATGACAAC 
CACGCCAAAT GCGGTCTGGT ATGGGTACCG CATGTGGTGG CCTACAGGTG CCGCACTTGC 
GGCATATCGC CCTGCATGTC CATATGCCGG GACTGCTTCA AGAAGGGCAA CCACACGAAC 
CACGACTTCA ATATGTTCCT GTCACAGGCG GGCGGTGCCT GCGATTGTGG TGACACCTCG 
GTCATGAAGG CGGAGGGTTT TTGCAGTGAT CATGGCATCA ATAATCGGGT GAATCGCGAT 
CCGGTGCCAA ACAATCTTTT GGCAGTGGCC GAGGCCATAA TGCCCAAGCT GCTCTTCCGC 
CTGCTGCAGC ACTTCCGTGA GCACAGCGAC ACCCCACTGG AGGTGCAAGC GATAACGTCG 
TACTCCTGCG AGGAGTTCGC CAACATGCTG ATCGATCTGA ACAACATGGG CGAAATAATG 
CGCAAAGTGA TGACGCGCAC GCTGATAAAT CCGGAGGTGT ATGCCTTCTT CATGGAAGCA 
CCGTGCCAGG ACACCCGGAA TGGACGCTTC TTGAAGGCGA ACCGAGAGAA GTACGAGGAT 
GCCGTCAATA GATTCCCAAA CCCAGAGCCA CCCGATGAGT ACAGAGATCT GCCCGCGCTG 
GGCGACAAAT TGGTCCACAC CACGCTACTC GAAGAGTTCA TCTTCTGGAC ATTTAAGTTC 
GAGTTTCCAC AGACTCTGGT CTGCTTTCTG CTGAACATGC TGCCTGATCA GGATTACAAG 
GAACACCTCA CCCGCACATT TGTGATGCAC TACAGCCGCA TACCGTCCGT GTTGGAAATG 
TCCCGTGATC CGGACACGCT CAGCAACCGG GTGGTCCACA TGAGCGTCCA ACTCTTCTCT 
AACGAAAGTC TGGCCCTCAA GATGGTCAAC GAGCTGTCGC TGCTGCACGT AATGATTATT 
AGTCTGAAGC TAATGATGTC CAAGATACTC ATACAGAATA CATTGCATGA TCCCAACAAG 
AATTTTCATT TCGTCATCGA CTGCACGCGT CAGGTGATGA AGGACCACTG CTACTGGCCA 
CTAGTCTCGG ACTTTAACAA CGTCCTTTCA CACGAATCGG TGGCCCTGGT CTTTCTGCGG 
GACGACAATC TCATCGATAT GTGGTTCCAG TTTCTCCAGA TGCTCCAGGG CATGAATGTC 
AATGTGCGCG AGACGGCTTC TCATGTGGAA TTCGAGCCAA ATAGCTACTA TGCGGCGTTC 
TCCTGTGAGC TTGAGGCCAG TGCCTATCCC ATGTGGTCGA TTATCTCGCA TCTGCAGGAT 
GGCACACATG CCCATCTGGC AAAGAAGATC ATCAACTACT GTGTGACGAC GCTGCACGAG 
TGGCTAGACT CTATTTACTT TATGGAGGCG CGTCTATCTA TGGAGGAGAT GATGCAGGCT 
TCGTTCCACT TTCCCCTGCA TCGTTATCTG GCTGCCTTTG TTTGCCAGGC GGTAACTAAA 
ATGGGAATCA GTCTGAACGA TGTGCTGCCC TCACGCCCCT ATCTGCTGCC CCTGCTGATG 
ATCCATCCAC TTCGCGTCCA GAGTTTTTTC TACGAGATCC TGGCCGGAAA ATGGGTGCGC 
AATGGTCTCC AAATCAAGGG ACAAGCGATG ACCTATATCC AGGCGAACTT CTGCAACTCG 
ATGGCCGACA TGGACCTGTT CTTCCTGCAA ATCTGTGCCA CCAACCTGCC GCAGTACTTC 
TTTCTGCAGA ACACCATCGA GTTGTTCGAC GTAGGTCAAT GGCTGGAGAC AGCTCCCTTG 
AAGCAGCCTC AAAAGGCGGA GCAATCCTCG ATGCTGGAGG GATTCCTAAC CTTCTTGGCC 
ACCCTGGTGA CGAGTCGCAC TAATCTGGGA AACGATGAAG CCACCCAATG CATTATTGAG 
ATCAGTGCCT TGTTGGCTAC CGAGAACAAG ACGCATTCGC AGCTCCTTGA ACTAATGCCG 
GAGCGGTCGG GCAATGTGCA TACCAAAAAC TTTGAGACCT TCCTTAAGAA GCTATCGGTT 
TACAAGGCTC CGTCGAGTGG GAGCGAGAAC CTGGAGCAGG GTCTCTTCAC GCCCATCGAC 
GAGGTGTGGG AGAAGCACTA CGATCCGCTG CACGTCCTTT TGCGAGCAGT TCATCGTCGA 
GACTTTCAAT CATCGCTGGA CCGCTTTACA AACTATGTGA AGAGCAAGGA TAAGATGCCG 
GCAAGTGGTA ACCTATGGCC ACCCTTCCGA TTGCCTCATG CTCTGCCCGC AACGAGCTCC 
TTCAGTGATC CCTGCAAGAT TTTGAACTCG CGCATCTTTC ACTCCACCAT CCTGTCGATA 
TTTTTCCGTG CAGTTCACAC ACGTGATGTT TCCGAGCATC TGCTGGCTCT GGCTGTGTTC 
CTCTTAGAGA TTGCTGTGGA AACAAGTGAT GATGTTGGCA GTGGAACTGG AGATCGGGCC 
CCTCCTGCGC TGGCTGCTGT GGTGGAGTCA TCTAGCGGGC CGGGCTATCA TGGCTACGGC 
ACGGGACGGC ATGAGCCGCC CAAGCTTTTC CACTGCTATC CCACGGACAA TCTAAGCTGC 
AATCTGCGCC ACGTGGTCAA GAAAGTGTCG CTGAAGTCAC GCGATCCGCA AGTGATCACC 
TCCAGCTATC GGTCGAACCC ATTTTACTCA GATCTGGACT TTGAGGTTGA AGCGGATCCA 
GAGCAATCGA TGCGCATGAT TGGTCAAGGG GACCCCGAAG GAGATGAGGC AACGGGTGGA 
GCTGGACTGG GAATGGGAGC TCATAGCAGA AGGAATGTCT CTCAGGCGCT GGTGCCGATG 
AGAGTGCCCG GCATGGAGGT CGCCCTACCA CCGGATCTTT CAGTAGTTGC CGAAACCGGT 
GTGGTTATCC GCCAGGATAG CAACGAAGAT GATCTACTGA GAGAAGGCAA TCATGCCATG 
GATATGAGTC CACCGGCTGC TCTCGATTTC CACTTTCCGC TGCAGCAGAT CACTTTGCCG 
GAGAGCGGAA TGGAGGTGGC TATTCGGAGA GATCTTCTGC TAGCCGAAAC GAATAATATG 
GGCGCAATTG CAGGTGGAGC TGGAGGTGGC GCCAATGCAT CGGCCACACC ACCAGCAGGG 
GCTTCCAATG AGATGTTCTC GCCTACCACA CCCACAGGCA GCGGTATGCT CCTGCCTTTC 
CAGCGGGTTC AGCCTGTAGC CGTACCCAGT AGCGGTAATA TGGATATTGT ACCATCAAAT 
GCCATGGGTG CCGGCGGAAG TTTCACATCC GGCGTAAGTG GTAGCGGCAC TGGACGTAGA 
ATGAACTACG AAACGGGTGG AGCACGTAAG CGATCCGTGG ACATTGCCAT CGGTGGCAGC 
AATAAGGATG AGCTGCATTT GGACGAAAGC ATTTTGTCCC TGCTACTAAA GCTTCATTCT 
CAGCTCTCAG GCACACTAGA TAGCTTCTCG TTGAGCGACG GTGAAGATCA ATCATCTGAC 
GACGATTCGA CCATGGATGT GGACTGTAAC GAAGCCAGCA CCTCCATGGC AGCAGCGGAG 
AGCACTGCCC TGGCTGAGAG AGGACGCAGT AAGCGCAACT ACAAGAACAT TCATGTATCC 
TCCTCGCGCA TCGGTGACGG ACCATTCTTT ATTGGTAATC TGCTGCGAAA GATCGCCAAG 
CAGGATGAGC AGTGCGCCCA GAGCATCGAC GACATCCGTG CCAGACTCTG GCCGAATCAG 
CGTGAAAAAC AAGCAGAGGC GAAAGCACGC GAGGCCAAGG AGAAGGAGGA GCGCCGCAAA 
AAAGCACGTG AGCGCCAGCA GAAGATGATG CAAGACTTCG CCAACAAGCA GAAGCTCTTC 
ATGCAATCCG CTGCAGCGTC ATCCAGTGGC ATGGGCTACG GACCGGAGGA CGAGGACGAT 
GAGGAGTTGT ACGAGGAGCA GCCGCGTGAG AAAGAGTACG ATTGCATAAT CTGCAATTGC 
ACTACGCCCA GTACCGAATC GAATCCTATC GGCTTGGTCG TTCTGGTCGA GTCCAGCGGC 
ATTGTTGGTC ATCGGCGGCG CATCGCCGAG CGATTGCCGC TGCCGATAAA CGCGGAGGAT 
GAGTCCCGAC TGGCGCACAC CACTCGACTG GCGGCTGAAT TCAACAGGCG CACGGAACTG 
CTCAGTCTGA AGTTTGGCGA TGAGTCCTGG TATCTGTCCA ATAATATGGC CTACGACAAC 
GGTGTACATG TGCAGTCGTG TGGCCACCAC GTTCATCTCA GTTGCCTAGA GGCATACCTT 
AAGACGCTGT ACACCACCCA ACGTCAGCCA GTTCAGGATC GTGGCGAGTT CTACTGTCCC 
GTGTGCCGAC AGCTCTCAAA CTCGGTGCTG CCGCTGAGTC CACAACTGGA CAGACCCACG 
CACCTGGTCC GCTCTGGTAA TCAGCCATTC GAACGTCTGG TCGCCGATCT AACAGATCTG 
ATTAAAGAGA ACGAAACGAT ACCGCAGCCA ACAAAACTGA CAGAGGCCAT GGGCCATGCC 
ATGGAGGTGA TGACAAACAT TGCCCAGCGC AAGGTTAAAT GCTCCTCGAT AACGTTCCGC 
AAGCTGTTCA TTTTCGTTAC CTCGATTGCG CGTACAAACC TGGAGGCGGA GATCATCCAG 
CGGGGCGGAT CACTATGTAC AGCGAATGCC ACGCGGTACA AGCCAAAGCG GGAATGCATC 
GTACCGCTAC TGCACGTGCT ATCGGTACAT GTGCGTGTCT TGGTTGAATG GCCGCTGTGG 
AGCAGCTGGG CCTCGTTAGC TGGCCTGCCC GTTACCGCTA CGGAACCGTT GCCGGCGCAC 
TGCCTCGAAT TGATTCCCAG CATATTAGCG GATCCCATTG CTCTGCTGCT CAAGTTTATC 
CTGCTGGCGC CACTGCAACT CGATCAGGAT TACTTTACGT GCATGGTGAA AGTCATGTAC 
AATCTGCTGT ACTATCAAAT TGTGGTTCAG CTGTGCGTTA CTCTCACCGA TTTAGAGTGC 
GATCACATTG TCAAAGTTTA CGGCAGCACT AGCGTTGGCA GTGACAACTC TGCTGCGGAA 
TCCCAACAGC AGGAGTCTGC CGCCGGGACA ACGAACAACC GTCGCCGTGC TGGACAACAG 
CAGCAGAGCT CCTCGCAGCT AGGAAAGGCC ATGGCCCTGG TGCTGAGCCA GACAAATGAC 
CTAGTTCACT TGCGACGCGA CTGCATACCC AGTACCAGCA GCTCGGCGGC AGCGTCGGCG 
GCTGGTTCCT CCTCCACAAC GTCCACAAAC CATGGAGCTA GCGCAGCCAC AGCCTCCTCT 
GCCACGACGA TCGAGGTTAA TCTGAAGTCC ATGGAATTAC AGCTGCAGGC GCTATGCTTG 
CCGTTTTTGC GAGTAGCTGC CCTGCTGCGC CAGCATCTCT ATCGCCATGA GATGCCGGAG 
ATCTCGGCAC CGGGACTGGA ATTTGTGCGC CTTGTTTACT ATTTGGAACT CGTAACAGAT 
TCTATGGACT GGGATTGCTT TAATGCCAGC AAGGGGCTAT GCTTTATTCC CGGCACTGAG 
ACAACGCTGC CCCAGTTCTG GTGCCAGCAG TTGATGGAGG TGCGTCCGCC GGCCGATACG 
GTCAGAGAAT TGGTGCTCAT CAATCAGCAT TCGTTGTGGC AGCAGCCGCG ACTTCTTGAA 
TTGCCGCGCG AGTACGAACG CTTGTTCACG TACTACCATG AGCGGCCGTG CCTCAATTGC 
TATAAAGTGC CCAAGGAGAG CTCCATCTGC CTGCTGTGCG GGACGATCGT ATGCCTCAAG 
CAGAACTGCT GCGCGGAGAA TGACTGCTGC GAGGCTGTTC GCCATACGCT ATCCTGTGGC 
GGTGGCATAG GCATCTTTTT GGTGGTCACC TCCACGTACA TCATTGTTAT CCGCGGACGA 
CGCGCCTGCC TTTGGGGCTC ACTCTATCTG GACGACTTCG ATGAGGAGGA TCGCGATCTC 
AAGCGCGGCA AACCTTTGTA TTTGAGCAAA GACCGCTTCA ATCTCCTGGA GTCGCAGTGG 
CTGTCCCATA AGTTTGCTCA TACAAAACAC ACATGGGTGT TTCACCGGGA TCTCTTGTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OFa03020
Clone ID Related Accession (Same CDS sequence) NM_001272388.1
Accession Version NM_001272388.1 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 6711bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2019-04-24
Organism Drosophila melanogaster(fruit fly)
Product Ubr3 ubiquitin ligase, isoform B
Comment Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END## 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
3721
3781
3841
3901
3961
4021
4081
4141
4201
4261
4321
4381
4441
4501
4561
4621
4681
4741
4801
4861
4921
4981
5041
5101
5161
5221
5281
5341
5401
5461
5521
5581
5641
5701
5761
5821
5881
5941
6001
6061
6121
6181
6241
6301
6361
6421
6481
6541
6601
6661
ATGGACGAGG ATGACAACCT GTCCAATGCC GACATAAGCA TAGATGACGA GGAGGTGGTG 
CGGGAGCAGA CCCACCATCC GCCCATGCAA GAGGATCAGG AGCTGGATAA CGAAGATGGG 
TCATCCGACG TGGATCTGTC TTCGGTGTAC GTCGTGCCAC CCATAGTCTC GGCACCATCG 
CCCGCATCAG TGCGCATCGC TGGTGGATCC ACAATTGGTG GCGGAGGGGT CGCAGCTGGA 
GCAGTTCCAA CAACCACCGA TAACTCGCAT TCCCCCTTCC ACGATTGGGA TTCCAGTGTG 
GCGGCCGCTG CTGCTCCACC GCCGCCACCG CCCAGGACAG GACCAACTAC AACTACAAGC 
TCCGGCACAG CCGCGGAATC GGGAGCCGCG TCCGCCTTAT CCGACGATGC CGGCGCCAAG 
CCAAGTTTCT TCTTTGGCGC CTCCTCGTTC AGTTTGCGCA GCCGCAAGGA GGTGGCCGCC 
CTGATCAACA CGGAATGCTG CCGTGGCAGC CCCACACCCG ATCTCGACTC CATAATGGAT 
ACCCTATTCA ATCCGGGGAC GCCCATCGAC AATCTGGACA ACATCGAGTG GATACGATGG 
CTCATTGCCG GCGGACGGAC GCCGCAGGAG TTTGTTAAAA TTGTGCGCAG CTATGACAAC 
CACGCCAAAT GCGGTCTGGT ATGGGTACCG CATGTGGTGG CCTACAGGTG CCGCACTTGC 
GGCATATCGC CCTGCATGTC CATATGCCGG GACTGCTTCA AGAAGGGCAA CCACACGAAC 
CACGACTTCA ATATGTTCCT GTCACAGGCG GGCGGTGCCT GCGATTGTGG TGACACCTCG 
GTCATGAAGG CGGAGGGTTT TTGCAGTGAT CATGGCATCA ATAATCGGGT GAATCGCGAT 
CCGGTGCCAA ACAATCTTTT GGCAGTGGCC GAGGCCATAA TGCCCAAGCT GCTCTTCCGC 
CTGCTGCAGC ACTTCCGTGA GCACAGCGAC ACCCCACTGG AGGTGCAAGC GATAACGTCG 
TACTCCTGCG AGGAGTTCGC CAACATGCTG ATCGATCTGA ACAACATGGG CGAAATAATG 
CGCAAAGTGA TGACGCGCAC GCTGATAAAT CCGGAGGTGT ATGCCTTCTT CATGGAAGCA 
CCGTGCCAGG ACACCCGGAA TGGACGCTTC TTGAAGGCGA ACCGAGAGAA GTACGAGGAT 
GCCGTCAATA GATTCCCAAA CCCAGAGCCA CCCGATGAGT ACAGAGATCT GCCCGCGCTG 
GGCGACAAAT TGGTCCACAC CACGCTACTC GAAGAGTTCA TCTTCTGGAC ATTTAAGTTC 
GAGTTTCCAC AGACTCTGGT CTGCTTTCTG CTGAACATGC TGCCTGATCA GGATTACAAG 
GAACACCTCA CCCGCACATT TGTGATGCAC TACAGCCGCA TACCGTCCGT GTTGGAAATG 
TCCCGTGATC CGGACACGCT CAGCAACCGG GTGGTCCACA TGAGCGTCCA ACTCTTCTCT 
AACGAAAGTC TGGCCCTCAA GATGGTCAAC GAGCTGTCGC TGCTGCACGT AATGATTATT 
AGTCTGAAGC TAATGATGTC CAAGATACTC ATACAGAATA CATTGCATGA TCCCAACAAG 
AATTTTCATT TCGTCATCGA CTGCACGCGT CAGGTGATGA AGGACCACTG CTACTGGCCA 
CTAGTCTCGG ACTTTAACAA CGTCCTTTCA CACGAATCGG TGGCCCTGGT CTTTCTGCGG 
GACGACAATC TCATCGATAT GTGGTTCCAG TTTCTCCAGA TGCTCCAGGG CATGAATGTC 
AATGTGCGCG AGACGGCTTC TCATGTGGAA TTCGAGCCAA ATAGCTACTA TGCGGCGTTC 
TCCTGTGAGC TTGAGGCCAG TGCCTATCCC ATGTGGTCGA TTATCTCGCA TCTGCAGGAT 
GGCACACATG CCCATCTGGC AAAGAAGATC ATCAACTACT GTGTGACGAC GCTGCACGAG 
TGGCTAGACT CTATTTACTT TATGGAGGCG CGTCTATCTA TGGAGGAGAT GATGCAGGCT 
TCGTTCCACT TTCCCCTGCA TCGTTATCTG GCTGCCTTTG TTTGCCAGGC GGTAACTAAA 
ATGGGAATCA GTCTGAACGA TGTGCTGCCC TCACGCCCCT ATCTGCTGCC CCTGCTGATG 
ATCCATCCAC TTCGCGTCCA GAGTTTTTTC TACGAGATCC TGGCCGGAAA ATGGGTGCGC 
AATGGTCTCC AAATCAAGGG ACAAGCGATG ACCTATATCC AGGCGAACTT CTGCAACTCG 
ATGGCCGACA TGGACCTGTT CTTCCTGCAA ATCTGTGCCA CCAACCTGCC GCAGTACTTC 
TTTCTGCAGA ACACCATCGA GTTGTTCGAC GTAGGTCAAT GGCTGGAGAC AGCTCCCTTG 
AAGCAGCCTC AAAAGGCGGA GCAATCCTCG ATGCTGGAGG GATTCCTAAC CTTCTTGGCC 
ACCCTGGTGA CGAGTCGCAC TAATCTGGGA AACGATGAAG CCACCCAATG CATTATTGAG 
ATCAGTGCCT TGTTGGCTAC CGAGAACAAG ACGCATTCGC AGCTCCTTGA ACTAATGCCG 
GAGCGGTCGG GCAATGTGCA TACCAAAAAC TTTGAGACCT TCCTTAAGAA GCTATCGGTT 
TACAAGGCTC CGTCGAGTGG GAGCGAGAAC CTGGAGCAGG GTCTCTTCAC GCCCATCGAC 
GAGGTGTGGG AGAAGCACTA CGATCCGCTG CACGTCCTTT TGCGAGCAGT TCATCGTCGA 
GACTTTCAAT CATCGCTGGA CCGCTTTACA AACTATGTGA AGAGCAAGGA TAAGATGCCG 
GCAAGTGGTA ACCTATGGCC ACCCTTCCGA TTGCCTCATG CTCTGCCCGC AACGAGCTCC 
TTCAGTGATC CCTGCAAGAT TTTGAACTCG CGCATCTTTC ACTCCACCAT CCTGTCGATA 
TTTTTCCGTG CAGTTCACAC ACGTGATGTT TCCGAGCATC TGCTGGCTCT GGCTGTGTTC 
CTCTTAGAGA TTGCTGTGGA AACAAGTGAT GATGTTGGCA GTGGAACTGG AGATCGGGCC 
CCTCCTGCGC TGGCTGCTGT GGTGGAGTCA TCTAGCGGGC CGGGCTATCA TGGCTACGGC 
ACGGGACGGC ATGAGCCGCC CAAGCTTTTC CACTGCTATC CCACGGACAA TCTAAGCTGC 
AATCTGCGCC ACGTGGTCAA GAAAGTGTCG CTGAAGTCAC GCGATCCGCA AGTGATCACC 
TCCAGCTATC GGTCGAACCC ATTTTACTCA GATCTGGACT TTGAGGTTGA AGCGGATCCA 
GAGCAATCGA TGCGCATGAT TGGTCAAGGG GACCCCGAAG GAGATGAGGC AACGGGTGGA 
GCTGGACTGG GAATGGGAGC TCATAGCAGA AGGAATGTCT CTCAGGCGCT GGTGCCGATG 
AGAGTGCCCG GCATGGAGGT CGCCCTACCA CCGGATCTTT CAGTAGTTGC CGAAACCGGT 
GTGGTTATCC GCCAGGATAG CAACGAAGAT GATCTACTGA GAGAAGGCAA TCATGCCATG 
GATATGAGTC CACCGGCTGC TCTCGATTTC CACTTTCCGC TGCAGCAGAT CACTTTGCCG 
GAGAGCGGAA TGGAGGTGGC TATTCGGAGA GATCTTCTGC TAGCCGAAAC GAATAATATG 
GGCGCAATTG CAGGTGGAGC TGGAGGTGGC GCCAATGCAT CGGCCACACC ACCAGCAGGG 
GCTTCCAATG AGATGTTCTC GCCTACCACA CCCACAGGCA GCGGTATGCT CCTGCCTTTC 
CAGCGGGTTC AGCCTGTAGC CGTACCCAGT AGCGGTAATA TGGATATTGT ACCATCAAAT 
GCCATGGGTG CCGGCGGAAG TTTCACATCC GGCGTAAGTG GTAGCGGCAC TGGACGTAGA 
ATGAACTACG AAACGGGTGG AGCACGTAAG CGATCCGTGG ACATTGCCAT CGGTGGCAGC 
AATAAGGATG AGCTGCATTT GGACGAAAGC ATTTTGTCCC TGCTACTAAA GCTTCATTCT 
CAGCTCTCAG GCACACTAGA TAGCTTCTCG TTGAGCGACG GTGAAGATCA ATCATCTGAC 
GACGATTCGA CCATGGATGT GGACTGTAAC GAAGCCAGCA CCTCCATGGC AGCAGCGGAG 
AGCACTGCCC TGGCTGAGAG AGGACGCAGT AAGCGCAACT ACAAGAACAT TCATGTATCC 
TCCTCGCGCA TCGGTGACGG ACCATTCTTT ATTGGTAATC TGCTGCGAAA GATCGCCAAG 
CAGGATGAGC AGTGCGCCCA GAGCATCGAC GACATCCGTG CCAGACTCTG GCCGAATCAG 
CGTGAAAAAC AAGCAGAGGC GAAAGCACGC GAGGCCAAGG AGAAGGAGGA GCGCCGCAAA 
AAAGCACGTG AGCGCCAGCA GAAGATGATG CAAGACTTCG CCAACAAGCA GAAGCTCTTC 
ATGCAATCCG CTGCAGCGTC ATCCAGTGGC ATGGGCTACG GACCGGAGGA CGAGGACGAT 
GAGGAGTTGT ACGAGGAGCA GCCGCGTGAG AAAGAGTACG ATTGCATAAT CTGCAATTGC 
ACTACGCCCA GTACCGAATC GAATCCTATC GGCTTGGTCG TTCTGGTCGA GTCCAGCGGC 
ATTGTTGGTC ATCGGCGGCG CATCGCCGAG CGATTGCCGC TGCCGATAAA CGCGGAGGAT 
GAGTCCCGAC TGGCGCACAC CACTCGACTG GCGGCTGAAT TCAACAGGCG CACGGAACTG 
CTCAGTCTGA AGTTTGGCGA TGAGTCCTGG TATCTGTCCA ATAATATGGC CTACGACAAC 
GGTGTACATG TGCAGTCGTG TGGCCACCAC GTTCATCTCA GTTGCCTAGA GGCATACCTT 
AAGACGCTGT ACACCACCCA ACGTCAGCCA GTTCAGGATC GTGGCGAGTT CTACTGTCCC 
GTGTGCCGAC AGCTCTCAAA CTCGGTGCTG CCGCTGAGTC CACAACTGGA CAGACCCACG 
CACCTGGTCC GCTCTGGTAA TCAGCCATTC GAACGTCTGG TCGCCGATCT AACAGATCTG 
ATTAAAGAGA ACGAAACGAT ACCGCAGCCA ACAAAACTGA CAGAGGCCAT GGGCCATGCC 
ATGGAGGTGA TGACAAACAT TGCCCAGCGC AAGGTTAAAT GCTCCTCGAT AACGTTCCGC 
AAGCTGTTCA TTTTCGTTAC CTCGATTGCG CGTACAAACC TGGAGGCGGA GATCATCCAG 
CGGGGCGGAT CACTATGTAC AGCGAATGCC ACGCGGTACA AGCCAAAGCG GGAATGCATC 
GTACCGCTAC TGCACGTGCT ATCGGTACAT GTGCGTGTCT TGGTTGAATG GCCGCTGTGG 
AGCAGCTGGG CCTCGTTAGC TGGCCTGCCC GTTACCGCTA CGGAACCGTT GCCGGCGCAC 
TGCCTCGAAT TGATTCCCAG CATATTAGCG GATCCCATTG CTCTGCTGCT CAAGTTTATC 
CTGCTGGCGC CACTGCAACT CGATCAGGAT TACTTTACGT GCATGGTGAA AGTCATGTAC 
AATCTGCTGT ACTATCAAAT TGTGGTTCAG CTGTGCGTTA CTCTCACCGA TTTAGAGTGC 
GATCACATTG TCAAAGTTTA CGGCAGCACT AGCGTTGGCA GTGACAACTC TGCTGCGGAA 
TCCCAACAGC AGGAGTCTGC CGCCGGGACA ACGAACAACC GTCGCCGTGC TGGACAACAG 
CAGCAGAGCT CCTCGCAGCT AGGAAAGGCC ATGGCCCTGG TGCTGAGCCA GACAAATGAC 
CTAGTTCACT TGCGACGCGA CTGCATACCC AGTACCAGCA GCTCGGCGGC AGCGTCGGCG 
GCTGGTTCCT CCTCCACAAC GTCCACAAAC CATGGAGCTA GCGCAGCCAC AGCCTCCTCT 
GCCACGACGA TCGAGGTTAA TCTGAAGTCC ATGGAATTAC AGCTGCAGGC GCTATGCTTG 
CCGTTTTTGC GAGTAGCTGC CCTGCTGCGC CAGCATCTCT ATCGCCATGA GATGCCGGAG 
ATCTCGGCAC CGGGACTGGA ATTTGTGCGC CTTGTTTACT ATTTGGAACT CGTAACAGAT 
TCTATGGACT GGGATTGCTT TAATGCCAGC AAGGGGCTAT GCTTTATTCC CGGCACTGAG 
ACAACGCTGC CCCAGTTCTG GTGCCAGCAG TTGATGGAGG TGCGTCCGCC GGCCGATACG 
GTCAGAGAAT TGGTGCTCAT CAATCAGCAT TCGTTGTGGC AGCAGCCGCG ACTTCTTGAA 
TTGCCGCGCG AGTACGAACG CTTGTTCACG TACTACCATG AGCGGCCGTG CCTCAATTGC 
TATAAAGTGC CCAAGGAGAG CTCCATCTGC CTGCTGTGCG GGACGATCGT ATGCCTCAAG 
CAGAACTGCT GCGCGGAGAA TGACTGCTGC GAGGCTGTTC GCCATACGCT ATCCTGTGGC 
GGTGGCATAG GCATCTTTTT GGTGGTCACC TCCACGTACA TCATTGTTAT CCGCGGACGA 
CGCGCCTGCC TTTGGGGCTC ACTCTATCTG GACGACTTCG ATGAGGAGGA TCGCGATCTC 
AAGCGCGGCA AACCTTTGTA TTTGAGCAAA GACCGCTTCA ATCTCCTGGA GTCGCAGTGG 
CTGTCCCATA AGTTTGCTCA TACAAAACAC ACATGGGTGT TTCACCGGGA TCTCTTGTGA 
AATATGGAAG ATCTACTGCG CAGCATCCAG GAGCTGGTTG GCCAGATCTG A

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001259317.1
CDS1074..7784
Translation

Target ORF information:

RefSeq Version NM_001272388.1
Organism Drosophila melanogaster(fruit fly)
Definition Drosophila melanogaster Ubr3 ubiquitin ligase (Ubr3), transcript variant B, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 NM_001272388.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
3721
3781
3841
3901
3961
4021
4081
4141
4201
4261
4321
4381
4441
4501
4561
4621
4681
4741
4801
4861
4921
4981
5041
5101
5161
5221
5281
5341
5401
5461
5521
5581
5641
5701
5761
5821
5881
5941
6001
6061
6121
6181
6241
6301
6361
6421
6481
6541
6601
6661
ATGGACGAGG ATGACAACCT GTCCAATGCC GACATAAGCA TAGATGACGA GGAGGTGGTG 
CGGGAGCAGA CCCACCATCC GCCCATGCAA GAGGATCAGG AGCTGGATAA CGAAGATGGG 
TCATCCGACG TGGATCTGTC TTCGGTGTAC GTCGTGCCAC CCATAGTCTC GGCACCATCG 
CCCGCATCAG TGCGCATCGC TGGTGGATCC ACAATTGGTG GCGGAGGGGT CGCAGCTGGA 
GCAGTTCCAA CAACCACCGA TAACTCGCAT TCCCCCTTCC ACGATTGGGA TTCCAGTGTG 
GCGGCCGCTG CTGCTCCACC GCCGCCACCG CCCAGGACAG GACCAACTAC AACTACAAGC 
TCCGGCACAG CCGCGGAATC GGGAGCCGCG TCCGCCTTAT CCGACGATGC CGGCGCCAAG 
CCAAGTTTCT TCTTTGGCGC CTCCTCGTTC AGTTTGCGCA GCCGCAAGGA GGTGGCCGCC 
CTGATCAACA CGGAATGCTG CCGTGGCAGC CCCACACCCG ATCTCGACTC CATAATGGAT 
ACCCTATTCA ATCCGGGGAC GCCCATCGAC AATCTGGACA ACATCGAGTG GATACGATGG 
CTCATTGCCG GCGGACGGAC GCCGCAGGAG TTTGTTAAAA TTGTGCGCAG CTATGACAAC 
CACGCCAAAT GCGGTCTGGT ATGGGTACCG CATGTGGTGG CCTACAGGTG CCGCACTTGC 
GGCATATCGC CCTGCATGTC CATATGCCGG GACTGCTTCA AGAAGGGCAA CCACACGAAC 
CACGACTTCA ATATGTTCCT GTCACAGGCG GGCGGTGCCT GCGATTGTGG TGACACCTCG 
GTCATGAAGG CGGAGGGTTT TTGCAGTGAT CATGGCATCA ATAATCGGGT GAATCGCGAT 
CCGGTGCCAA ACAATCTTTT GGCAGTGGCC GAGGCCATAA TGCCCAAGCT GCTCTTCCGC 
CTGCTGCAGC ACTTCCGTGA GCACAGCGAC ACCCCACTGG AGGTGCAAGC GATAACGTCG 
TACTCCTGCG AGGAGTTCGC CAACATGCTG ATCGATCTGA ACAACATGGG CGAAATAATG 
CGCAAAGTGA TGACGCGCAC GCTGATAAAT CCGGAGGTGT ATGCCTTCTT CATGGAAGCA 
CCGTGCCAGG ACACCCGGAA TGGACGCTTC TTGAAGGCGA ACCGAGAGAA GTACGAGGAT 
GCCGTCAATA GATTCCCAAA CCCAGAGCCA CCCGATGAGT ACAGAGATCT GCCCGCGCTG 
GGCGACAAAT TGGTCCACAC CACGCTACTC GAAGAGTTCA TCTTCTGGAC ATTTAAGTTC 
GAGTTTCCAC AGACTCTGGT CTGCTTTCTG CTGAACATGC TGCCTGATCA GGATTACAAG 
GAACACCTCA CCCGCACATT TGTGATGCAC TACAGCCGCA TACCGTCCGT GTTGGAAATG 
TCCCGTGATC CGGACACGCT CAGCAACCGG GTGGTCCACA TGAGCGTCCA ACTCTTCTCT 
AACGAAAGTC TGGCCCTCAA GATGGTCAAC GAGCTGTCGC TGCTGCACGT AATGATTATT 
AGTCTGAAGC TAATGATGTC CAAGATACTC ATACAGAATA CATTGCATGA TCCCAACAAG 
AATTTTCATT TCGTCATCGA CTGCACGCGT CAGGTGATGA AGGACCACTG CTACTGGCCA 
CTAGTCTCGG ACTTTAACAA CGTCCTTTCA CACGAATCGG TGGCCCTGGT CTTTCTGCGG 
GACGACAATC TCATCGATAT GTGGTTCCAG TTTCTCCAGA TGCTCCAGGG CATGAATGTC 
AATGTGCGCG AGACGGCTTC TCATGTGGAA TTCGAGCCAA ATAGCTACTA TGCGGCGTTC 
TCCTGTGAGC TTGAGGCCAG TGCCTATCCC ATGTGGTCGA TTATCTCGCA TCTGCAGGAT 
GGCACACATG CCCATCTGGC AAAGAAGATC ATCAACTACT GTGTGACGAC GCTGCACGAG 
TGGCTAGACT CTATTTACTT TATGGAGGCG CGTCTATCTA TGGAGGAGAT GATGCAGGCT 
TCGTTCCACT TTCCCCTGCA TCGTTATCTG GCTGCCTTTG TTTGCCAGGC GGTAACTAAA 
ATGGGAATCA GTCTGAACGA TGTGCTGCCC TCACGCCCCT ATCTGCTGCC CCTGCTGATG 
ATCCATCCAC TTCGCGTCCA GAGTTTTTTC TACGAGATCC TGGCCGGAAA ATGGGTGCGC 
AATGGTCTCC AAATCAAGGG ACAAGCGATG ACCTATATCC AGGCGAACTT CTGCAACTCG 
ATGGCCGACA TGGACCTGTT CTTCCTGCAA ATCTGTGCCA CCAACCTGCC GCAGTACTTC 
TTTCTGCAGA ACACCATCGA GTTGTTCGAC GTAGGTCAAT GGCTGGAGAC AGCTCCCTTG 
AAGCAGCCTC AAAAGGCGGA GCAATCCTCG ATGCTGGAGG GATTCCTAAC CTTCTTGGCC 
ACCCTGGTGA CGAGTCGCAC TAATCTGGGA AACGATGAAG CCACCCAATG CATTATTGAG 
ATCAGTGCCT TGTTGGCTAC CGAGAACAAG ACGCATTCGC AGCTCCTTGA ACTAATGCCG 
GAGCGGTCGG GCAATGTGCA TACCAAAAAC TTTGAGACCT TCCTTAAGAA GCTATCGGTT 
TACAAGGCTC CGTCGAGTGG GAGCGAGAAC CTGGAGCAGG GTCTCTTCAC GCCCATCGAC 
GAGGTGTGGG AGAAGCACTA CGATCCGCTG CACGTCCTTT TGCGAGCAGT TCATCGTCGA 
GACTTTCAAT CATCGCTGGA CCGCTTTACA AACTATGTGA AGAGCAAGGA TAAGATGCCG 
GCAAGTGGTA ACCTATGGCC ACCCTTCCGA TTGCCTCATG CTCTGCCCGC AACGAGCTCC 
TTCAGTGATC CCTGCAAGAT TTTGAACTCG CGCATCTTTC ACTCCACCAT CCTGTCGATA 
TTTTTCCGTG CAGTTCACAC ACGTGATGTT TCCGAGCATC TGCTGGCTCT GGCTGTGTTC 
CTCTTAGAGA TTGCTGTGGA AACAAGTGAT GATGTTGGCA GTGGAACTGG AGATCGGGCC 
CCTCCTGCGC TGGCTGCTGT GGTGGAGTCA TCTAGCGGGC CGGGCTATCA TGGCTACGGC 
ACGGGACGGC ATGAGCCGCC CAAGCTTTTC CACTGCTATC CCACGGACAA TCTAAGCTGC 
AATCTGCGCC ACGTGGTCAA GAAAGTGTCG CTGAAGTCAC GCGATCCGCA AGTGATCACC 
TCCAGCTATC GGTCGAACCC ATTTTACTCA GATCTGGACT TTGAGGTTGA AGCGGATCCA 
GAGCAATCGA TGCGCATGAT TGGTCAAGGG GACCCCGAAG GAGATGAGGC AACGGGTGGA 
GCTGGACTGG GAATGGGAGC TCATAGCAGA AGGAATGTCT CTCAGGCGCT GGTGCCGATG 
AGAGTGCCCG GCATGGAGGT CGCCCTACCA CCGGATCTTT CAGTAGTTGC CGAAACCGGT 
GTGGTTATCC GCCAGGATAG CAACGAAGAT GATCTACTGA GAGAAGGCAA TCATGCCATG 
GATATGAGTC CACCGGCTGC TCTCGATTTC CACTTTCCGC TGCAGCAGAT CACTTTGCCG 
GAGAGCGGAA TGGAGGTGGC TATTCGGAGA GATCTTCTGC TAGCCGAAAC GAATAATATG 
GGCGCAATTG CAGGTGGAGC TGGAGGTGGC GCCAATGCAT CGGCCACACC ACCAGCAGGG 
GCTTCCAATG AGATGTTCTC GCCTACCACA CCCACAGGCA GCGGTATGCT CCTGCCTTTC 
CAGCGGGTTC AGCCTGTAGC CGTACCCAGT AGCGGTAATA TGGATATTGT ACCATCAAAT 
GCCATGGGTG CCGGCGGAAG TTTCACATCC GGCGTAAGTG GTAGCGGCAC TGGACGTAGA 
ATGAACTACG AAACGGGTGG AGCACGTAAG CGATCCGTGG ACATTGCCAT CGGTGGCAGC 
AATAAGGATG AGCTGCATTT GGACGAAAGC ATTTTGTCCC TGCTACTAAA GCTTCATTCT 
CAGCTCTCAG GCACACTAGA TAGCTTCTCG TTGAGCGACG GTGAAGATCA ATCATCTGAC 
GACGATTCGA CCATGGATGT GGACTGTAAC GAAGCCAGCA CCTCCATGGC AGCAGCGGAG 
AGCACTGCCC TGGCTGAGAG AGGACGCAGT AAGCGCAACT ACAAGAACAT TCATGTATCC 
TCCTCGCGCA TCGGTGACGG ACCATTCTTT ATTGGTAATC TGCTGCGAAA GATCGCCAAG 
CAGGATGAGC AGTGCGCCCA GAGCATCGAC GACATCCGTG CCAGACTCTG GCCGAATCAG 
CGTGAAAAAC AAGCAGAGGC GAAAGCACGC GAGGCCAAGG AGAAGGAGGA GCGCCGCAAA 
AAAGCACGTG AGCGCCAGCA GAAGATGATG CAAGACTTCG CCAACAAGCA GAAGCTCTTC 
ATGCAATCCG CTGCAGCGTC ATCCAGTGGC ATGGGCTACG GACCGGAGGA CGAGGACGAT 
GAGGAGTTGT ACGAGGAGCA GCCGCGTGAG AAAGAGTACG ATTGCATAAT CTGCAATTGC 
ACTACGCCCA GTACCGAATC GAATCCTATC GGCTTGGTCG TTCTGGTCGA GTCCAGCGGC 
ATTGTTGGTC ATCGGCGGCG CATCGCCGAG CGATTGCCGC TGCCGATAAA CGCGGAGGAT 
GAGTCCCGAC TGGCGCACAC CACTCGACTG GCGGCTGAAT TCAACAGGCG CACGGAACTG 
CTCAGTCTGA AGTTTGGCGA TGAGTCCTGG TATCTGTCCA ATAATATGGC CTACGACAAC 
GGTGTACATG TGCAGTCGTG TGGCCACCAC GTTCATCTCA GTTGCCTAGA GGCATACCTT 
AAGACGCTGT ACACCACCCA ACGTCAGCCA GTTCAGGATC GTGGCGAGTT CTACTGTCCC 
GTGTGCCGAC AGCTCTCAAA CTCGGTGCTG CCGCTGAGTC CACAACTGGA CAGACCCACG 
CACCTGGTCC GCTCTGGTAA TCAGCCATTC GAACGTCTGG TCGCCGATCT AACAGATCTG 
ATTAAAGAGA ACGAAACGAT ACCGCAGCCA ACAAAACTGA CAGAGGCCAT GGGCCATGCC 
ATGGAGGTGA TGACAAACAT TGCCCAGCGC AAGGTTAAAT GCTCCTCGAT AACGTTCCGC 
AAGCTGTTCA TTTTCGTTAC CTCGATTGCG CGTACAAACC TGGAGGCGGA GATCATCCAG 
CGGGGCGGAT CACTATGTAC AGCGAATGCC ACGCGGTACA AGCCAAAGCG GGAATGCATC 
GTACCGCTAC TGCACGTGCT ATCGGTACAT GTGCGTGTCT TGGTTGAATG GCCGCTGTGG 
AGCAGCTGGG CCTCGTTAGC TGGCCTGCCC GTTACCGCTA CGGAACCGTT GCCGGCGCAC 
TGCCTCGAAT TGATTCCCAG CATATTAGCG GATCCCATTG CTCTGCTGCT CAAGTTTATC 
CTGCTGGCGC CACTGCAACT CGATCAGGAT TACTTTACGT GCATGGTGAA AGTCATGTAC 
AATCTGCTGT ACTATCAAAT TGTGGTTCAG CTGTGCGTTA CTCTCACCGA TTTAGAGTGC 
GATCACATTG TCAAAGTTTA CGGCAGCACT AGCGTTGGCA GTGACAACTC TGCTGCGGAA 
TCCCAACAGC AGGAGTCTGC CGCCGGGACA ACGAACAACC GTCGCCGTGC TGGACAACAG 
CAGCAGAGCT CCTCGCAGCT AGGAAAGGCC ATGGCCCTGG TGCTGAGCCA GACAAATGAC 
CTAGTTCACT TGCGACGCGA CTGCATACCC AGTACCAGCA GCTCGGCGGC AGCGTCGGCG 
GCTGGTTCCT CCTCCACAAC GTCCACAAAC CATGGAGCTA GCGCAGCCAC AGCCTCCTCT 
GCCACGACGA TCGAGGTTAA TCTGAAGTCC ATGGAATTAC AGCTGCAGGC GCTATGCTTG 
CCGTTTTTGC GAGTAGCTGC CCTGCTGCGC CAGCATCTCT ATCGCCATGA GATGCCGGAG 
ATCTCGGCAC CGGGACTGGA ATTTGTGCGC CTTGTTTACT ATTTGGAACT CGTAACAGAT 
TCTATGGACT GGGATTGCTT TAATGCCAGC AAGGGGCTAT GCTTTATTCC CGGCACTGAG 
ACAACGCTGC CCCAGTTCTG GTGCCAGCAG TTGATGGAGG TGCGTCCGCC GGCCGATACG 
GTCAGAGAAT TGGTGCTCAT CAATCAGCAT TCGTTGTGGC AGCAGCCGCG ACTTCTTGAA 
TTGCCGCGCG AGTACGAACG CTTGTTCACG TACTACCATG AGCGGCCGTG CCTCAATTGC 
TATAAAGTGC CCAAGGAGAG CTCCATCTGC CTGCTGTGCG GGACGATCGT ATGCCTCAAG 
CAGAACTGCT GCGCGGAGAA TGACTGCTGC GAGGCTGTTC GCCATACGCT ATCCTGTGGC 
GGTGGCATAG GCATCTTTTT GGTGGTCACC TCCACGTACA TCATTGTTAT CCGCGGACGA 
CGCGCCTGCC TTTGGGGCTC ACTCTATCTG GACGACTTCG ATGAGGAGGA TCGCGATCTC 
AAGCGCGGCA AACCTTTGTA TTTGAGCAAA GACCGCTTCA ATCTCCTGGA GTCGCAGTGG 
CTGTCCCATA AGTTTGCTCA TACAAAACAC ACATGGGTGT TTCACCGGGA TCTCTTGTGA 
AATATGGAAG ATCTACTGCG CAGCATCCAG GAGCTGGTTG GCCAGATCTG A

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OFa03021
Clone ID Related Accession (Same CDS sequence) NM_001272389.1
Accession Version NM_001272389.1 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 6657bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2019-04-24
Organism Drosophila melanogaster(fruit fly)
Product Ubr3 ubiquitin ligase, isoform C
Comment Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END## 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
3721
3781
3841
3901
3961
4021
4081
4141
4201
4261
4321
4381
4441
4501
4561
4621
4681
4741
4801
4861
4921
4981
5041
5101
5161
5221
5281
5341
5401
5461
5521
5581
5641
5701
5761
5821
5881
5941
6001
6061
6121
6181
6241
6301
6361
6421
6481
6541
6601
ATGGACGAGG ATGACAACCT GTCCAATGCC GACATAAGCA TAGATGACGA GGAGGTGGTG 
CGGGAGCAGA CCCACCATCC GCCCATGCAA GAGGATCAGG AGCTGGATAA CGAAGATGGG 
TCATCCGACG TGGATCTGTC TTCGGTGTAC GTCGTGCCAC CCATAGTCTC GGCACCATCG 
CCCGCATCAG TGCGCATCGC TGGTGGATCC ACAATTGGTG GCGGAGGGGT CGCAGCTGGA 
GCAGTTCCAA CAACCACCGA TAACTCGCAT TCCCCCTTCC ACGATTGGGA TTCCAGTGTG 
GCGGCCGCTG CTGCTCCACC GCCGCCACCG CCCAGGACAG GACCAACTAC AACTACAAGC 
TCCGGCACAG CCGCGGAATC GGGAGCCGCG TCCGCCTTAT CCGACGATGC CGGCGCCAAG 
CCAAGTTTCT TCTTTGGCGC CTCCTCGTTC AGTTTGCGCA GCCGCAAGGA GGTGGCCGCC 
CTGATCAACA CGGAATGCTG CCGTGGCAGC CCCACACCCG ATCTCGACTC CATAATGGAT 
ACCCTATTCA ATCCGGGGAC GCCCATCGAC AATCTGGACA ACATCGAGTG GATACGATGG 
CTCATTGCCG GCGGACGGAC GCCGCAGGAG TTTGTTAAAA TTGTGCGCAG CTATGACAAC 
CACGCCAAAT GCGGTCTGGT ATGGGTACCG CATGTGGTGG CCTACAGGTG CCGCACTTGC 
GGCATATCGC CCTGCATGTC CATATGCCGG GACTGCTTCA AGAAGGGCAA CCACACGAAC 
CACGACTTCA ATATGTTCCT GTCACAGGCG GGCGGTGCCT GCGATTGTGG TGACACCTCG 
GTCATGAAGG CGGAGGGTTT TTGCAGTGAT CATGGCATCA ATAATCGGGT GAATCGCGAT 
CCGGTGCCAA ACAATCTTTT GGCAGTGGCC GAGGCCATAA TGCCCAAGCT GCTCTTCCGC 
CTGCTGCAGC ACTTCCGTGA GCACAGCGAC ACCCCACTGG AGGTGCAAGC GATAACGTCG 
TACTCCTGCG AGGAGTTCGC CAACATGCTG ATCGATCTGA ACAACATGGG CGAAATAATG 
CGCAAAGTGA TGACGCGCAC GCTGATAAAT CCGGAGGTGT ATGCCTTCTT CATGGAAGCA 
CCGTGCCAGG ACACCCGGAA TGGACGCTTC TTGAAGGCGA ACCGAGAGAA GTACGAGGAT 
GCCGTCAATA GATTCCCAAA CCCAGAGCCA CCCGATGAGT ACAGAGATCT GCCCGCGCTG 
GGCGACAAAT TGGTCCACAC CACGCTACTC GAAGAGTTCA TCTTCTGGAC ATTTAAGTTC 
GAGTTTCCAC AGACTCTGGT CTGCTTTCTG CTGAACATGC TGCCTGATCA GGATTACAAG 
GAACACCTCA CCCGCACATT TGTGATGCAC TACAGCCGCA TACCGTCCGT GTTGGAAATG 
TCCCGTGATC CGGACACGCT CAGCAACCGG GTGGTCCACA TGAGCGTCCA ACTCTTCTCT 
AACGAAAGTC TGGCCCTCAA GATGGTCAAC GAGCTGTCGC TGCTGCACGT AATGATTATT 
AGTCTGAAGC TAATGATGTC CAAGATACTC ATACAGAATA CATTGCATGA TCCCAACAAG 
AATTTTCATT TCGTCATCGA CTGCACGCGT CAGGTGATGA AGGACCACTG CTACTGGCCA 
CTAGTCTCGG ACTTTAACAA CGTCCTTTCA CACGAATCGG TGGCCCTGGT CTTTCTGCGG 
GACGACAATC TCATCGATAT GTGGTTCCAG TTTCTCCAGA TGCTCCAGGG CATGAATGTC 
AATGTGCGCG AGACGGCTTC TCATGTGGAA TTCGAGCCAA ATAGCTACTA TGCGGCGTTC 
TCCTGTGAGC TTGAGGCCAG TGCCTATCCC ATGTGGTCGA TTATCTCGCA TCTGCAGGAT 
GGCACACATG CCCATCTGGC AAAGAAGATC ATCAACTACT GTGTGACGAC GCTGCACGAG 
TGGCTAGACT CTATTTACTT TATGGAGGCG CGTCTATCTA TGGAGGAGAT GATGCAGGCT 
TCGTTCCACT TTCCCCTGCA TCGTTATCTG GCTGCCTTTG TTTGCCAGGC GGTAACTAAA 
ATGGGAATCA GTCTGAACGA TGTGCTGCCC TCACGCCCCT ATCTGCTGCC CCTGCTGATG 
ATCCATCCAC TTCGCGTCCA GAGTTTTTTC TACGAGATCC TGGCCGGAAA ATGGGTGCGC 
AATGGTCTCC AAATCAAGGG ACAAGCGATG ACCTATATCC AGGCGAACTT CTGCAACTCG 
ATGGCCGACA TGGACCTGTT CTTCCTGCAA ATCTGTGCCA CCAACCTGCC GCAGTACTTC 
TTTCTGCAGA ACACCATCGA GTTGTTCGAC GTAGGTCAAT GGCTGGAGAC AGCTCCCTTG 
AAGCAGCCTC AAAAGGCGGA GCAATCCTCG ATGCTGGAGG GATTCCTAAC CTTCTTGGCC 
ACCCTGGTGA CGAGTCGCAC TAATCTGGGA AACGATGAAG CCACCCAATG CATTATTGAG 
ATCAGTGCCT TGTTGGCTAC CGAGAACAAG ACGCATTCGC AGCTCCTTGA ACTAATGCCG 
GAGCGGTCGG GCAATGTGCA TACCAAAAAC TTTGAGACCT TCCTTAAGAA GCTATCGGTT 
TACAAGGCTC CGTCGAGTGG GAGCGAGAAC CTGGAGCAGG GTCTCTTCAC GCCCATCGAC 
GAGGTGTGGG AGAAGCACTA CGATCCGCTG CACGTCCTTT TGCGAGCAGT TCATCGTCGA 
GACTTTCAAT CATCGCTGGA CCGCTTTACA AACTATGTGA AGAGCAAGGA TAAGATGCCG 
GCAAGTGGTA ACCTATGGCC ACCCTTCCGA TTGCCTCATG CTCTGCCCGC AACGAGCTCC 
TTCAGTGATC CCTGCAAGAT TTTGAACTCG CGCATCTTTC ACTCCACCAT CCTGTCGATA 
TTTTTCCGTG CAGTTCACAC ACGTGATGTT TCCGAGCATC TGCTGGCTCT GGCTGTGTTC 
CTCTTAGAGA TTGCTGTGGA AACAAGTGAT GATGTTGGCA GTGGAACTGG AGATCGGGCC 
CCTCCTGCGC TGGCTGCTGT GGTGGAGTCA TCTAGCGGGC CGGGCTATCA TGGCTACGGC 
ACGGGACGGC ATGAGCCGCC CAAGCTTTTC CACTGCTATC CCACGGACAA TCTAAGCTGC 
AATCTGCGCC ACGTGGTCAA GAAAGTGTCG CTGAAGTCAC GCGATCCGCA AGTGATCACC 
TCCAGCTATC GGTCGAACCC ATTTTACTCA GATCTGGACT TTGAGGTTGA AGCGGATCCA 
GAGCAATCGA TGCGCATGAT TGGTCAAGGG GACCCCGAAG GAGATGAGGC AACGGGTGGA 
GCTGGACTGG GAATGGGAGC TCATAGCAGA AGGAATGTCT CTCAGGCGCT GGTGCCGATG 
AGAGTGCCCG GCATGGAGGT CGCCCTACCA CCGGATCTTT CAGTAGTTGC CGAAACCGGT 
GTGGTTATCC GCCAGGATAG CAACGAAGAT GATCTACTGA GAGAAGGCAA TCATGCCATG 
GATATGAGTC CACCGGCTGC TCTCGATTTC CACTTTCCGC TGCAGCAGAT CACTTTGCCG 
GAGAGCGGAA TGGAGGTGGC TATTCGGAGA GATCTTCTGC TAGCCGAAAC GAATAATATG 
GGCGCAATTG CAGGTGGAGC TGGAGGTGGC GCCAATGCAT CGGCCACACC ACCAGCAGGG 
GCTTCCAATG AGATGTTCTC GCCTACCACA CCCACAGGCA GCGGTATGCT CCTGCCTTTC 
CAGCGGGTTC AGCCTGTAGC CGTACCCAGT AGCGGTAATA TGGATATTGT ACCATCAAAT 
GCCATGGGTG CCGGCGGAAG TTTCACATCC GGCGTAAGTG GTAGCGGCAC TGGACGTAGA 
ATGAACTACG AAACGGGTGG AGCACGTAAG CGATCCGTGG ACATTGCCAT CGGTGGCAGC 
AATAAGGATG AGCTGCATTT GGACGAAAGC ATTTTGTCCC TGCTACTAAA GCTTCATTCT 
CAGCTCTCAG GCACACTAGA TAGCTTCTCG TTGAGCGACG GTGAAGATCA ATCATCTGAC 
GACGATTCGA CCATGGATGT GGACTGTAAC GAAGCCAGCA CCTCCATGGC AGCAGCGGAG 
AGCACTGCCC TGGCTGAGAG AGGACGCAGT AAGCGCAACT ACAAGAACAT TCATGTATCC 
TCCTCGCGCA TCGGTGACGG ACCATTCTTT ATTGGTAATC TGCTGCGAAA GATCGCCAAG 
CAGGATGAGC AGTGCGCCCA GAGCATCGAC GACATCCGTG CCAGACTCTG GCCGAATCAG 
CGTGAAAAAC AAGCAGAGGC GAAAGCACGC GAGGCCAAGG AGAAGGAGGA GCGCCGCAAA 
AAAGCACGTG AGCGCCAGCA GAAGATGATG CAAGACTTCG CCAACAAGCA GAAGCTCTTC 
ATGCAATCCG CTGCAGCGTC ATCCAGTGGC ATGGGCTACG GACCGGAGGA CGAGGACGAT 
GAGGAGTTGT ACGAGGAGCA GCCGCGTGAG AAAGAGTACG ATTGCATAAT CTGCAATTGC 
ACTACGCCCA GTACCGAATC GAATCCTATC GGCTTGGTCG TTCTGGTCGA GTCCAGCGGC 
ATTGTTGGTC ATCGGCGGCG CATCGCCGAG CGATTGCCGC TGCCGATAAA CGCGGAGGAT 
GAGTCCCGAC TGGCGCACAC CACTCGACTG GCGGCTGAAT TCAACAGGCG CACGGAACTG 
CTCAGTCTGA AGTTTGGCGA TGAGTCCTGG TATCTGTCCA ATAATATGGC CTACGACAAC 
GGTGTACATG TGCAGTCGTG TGGCCACCAC GTTCATCTCA GTTGCCTAGA GGCATACCTT 
AAGACGCTGT ACACCACCCA ACGTCAGCCA GTTCAGGATC GTGGCGAGTT CTACTGTCCC 
GTGTGCCGAC AGCTCTCAAA CTCGGTGCTG CCGCTGAGTC CACAACTGGA CAGACCCACG 
CACCTGGTCC GCTCTGGTAA TCAGCCATTC GAACGTCTGG TCGCCGATCT AACAGATCTG 
ATTAAAGAGA ACGAAACGAT ACCGCCAACA AAACTGACAG AGGCCATGGG CCATGCCATG 
GAGGTGATGA CAAACATTGC CCAGCGCAAG GTTAAATGCT CCTCGATAAC GTTCCGCAAG 
CTGTTCATTT TCGTTACCTC GATTGCGCGT ACAAACCTGG AGGCGGAGAT CATCCAGCGG 
GGCGGATCAC TATGTACAGC GAATGCCACG CGGTACAAGC CAAAGCGGGA ATGCATCGTA 
CCGCTACTGC ACGTGCTATC GGTACATGTG CGTGTCTTGG TTGAATGGCC GCTGTGGAGC 
AGCTGGGCCT CGTTAGCTGG CCTGCCCGTT ACCGCTACGG AACCGTTGCC GGCGCACTGC 
CTCGAATTGA TTCCCAGCAT ATTAGCGGAT CCCATTGCTC TGCTGCTCAA GTTTATCCTG 
CTGGCGCCAC TGCAACTCGA TCAGGATTAC TTTACGTGCA TGGTGAAAGT CATGTACAAT 
CTGCTGTACT ATCAAATTGT GGTTCAGCTG TGCGTTACTC TCACCGATTT AGAGTGCGAT 
CACATTGTCA AAGTTTACGG CAGCACTAGC GTTGGCAGTG ACAACTCTGC TGCGGAATCC 
CAACAGCAGG AGTCTGCCGC CGGGACAACG AACAACCGTC GCCGTGCTGG ACAACAGCAG 
CAGAGCTCCT CGCAGCTAGG AAAGGCCATG GCCCTGGTGC TGAGCCAGAC AAATGACCTA 
GTTCACTTGC GACGCGACTG CATACCCAGT ACCAGCAGCT CGGCGGCAGC GTCGGCGGCT 
GGTTCCTCCT CCACAACGTC CACAAACCAT GGAGCTAGCG CAGCCACAGC CTCCTCTGCC 
ACGACGATCG AGGTTAATCT GAAGTCCATG GAATTACAGC TGCAGGCGCT ATGCTTGCCG 
TTTTTGCGAG TAGCTGCCCT GCTGCGCCAG CATCTCTATC GCCATGAGAT GCCGGAGATC 
TCGGCACCGG GACTGGAATT TGTGCGCCTT GTTTACTATT TGGAACTCGT AACAGATTCT 
ATGGACTGGG ATTGCTTTAA TGCCAGCAAG GGGCTATGCT TTATTCCCGG CACTGAGACA 
ACGCTGCCCC AGTTCTGGTG CCAGCAGTTG ATGGAGGTGC GTCCGCCGGC CGATACGGTC 
AGAGAATTGG TGCTCATCAA TCAGCATTCG TTGTGGCAGC AGCCGCGACT TCTTGAATTG 
CCGCGCGAGT ACGAACGCTT GTTCACGTAC TACCATGAGC GGCCGTGCCT CAATTGCTAT 
AAAGTGCCCA AGGAGAGCTC CATCTGCCTG CTGTGCGGGA CGATCGTATG CCTCAAGCAG 
AACTGCTGCG CGGAGAATGA CTGCTGCGAG GCTGTTCGCC ATACGCTATC CTGTGGCGGT 
GGCATAGGCA TCTTTTTGGT GGTCACCTCC ACGTACATCA TTGTTATCCG CGGACGACGC 
GCCTGCCTTT GGGGCTCACT CTATCTGGAC GACTTCGATG AGGAGGATCG CGATCTCAAG 
CGCGGCAAAC CTTTGTATTT GAGCAAAGAC CGCTTCAATC TCCTGGAGTC GCAGTGGCTG 
TCCCATAAGT TTGCTCATAC AAAACACACA TGGGTGTTTC ACCGGGATCT CTTGTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001259318.1
CDS1014..7670
Translation

Target ORF information:

RefSeq Version NM_001272389.1
Organism Drosophila melanogaster(fruit fly)
Definition Drosophila melanogaster Ubr3 ubiquitin ligase (Ubr3), transcript variant C, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 NM_001272389.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
3721
3781
3841
3901
3961
4021
4081
4141
4201
4261
4321
4381
4441
4501
4561
4621
4681
4741
4801
4861
4921
4981
5041
5101
5161
5221
5281
5341
5401
5461
5521
5581
5641
5701
5761
5821
5881
5941
6001
6061
6121
6181
6241
6301
6361
6421
6481
6541
6601
ATGGACGAGG ATGACAACCT GTCCAATGCC GACATAAGCA TAGATGACGA GGAGGTGGTG 
CGGGAGCAGA CCCACCATCC GCCCATGCAA GAGGATCAGG AGCTGGATAA CGAAGATGGG 
TCATCCGACG TGGATCTGTC TTCGGTGTAC GTCGTGCCAC CCATAGTCTC GGCACCATCG 
CCCGCATCAG TGCGCATCGC TGGTGGATCC ACAATTGGTG GCGGAGGGGT CGCAGCTGGA 
GCAGTTCCAA CAACCACCGA TAACTCGCAT TCCCCCTTCC ACGATTGGGA TTCCAGTGTG 
GCGGCCGCTG CTGCTCCACC GCCGCCACCG CCCAGGACAG GACCAACTAC AACTACAAGC 
TCCGGCACAG CCGCGGAATC GGGAGCCGCG TCCGCCTTAT CCGACGATGC CGGCGCCAAG 
CCAAGTTTCT TCTTTGGCGC CTCCTCGTTC AGTTTGCGCA GCCGCAAGGA GGTGGCCGCC 
CTGATCAACA CGGAATGCTG CCGTGGCAGC CCCACACCCG ATCTCGACTC CATAATGGAT 
ACCCTATTCA ATCCGGGGAC GCCCATCGAC AATCTGGACA ACATCGAGTG GATACGATGG 
CTCATTGCCG GCGGACGGAC GCCGCAGGAG TTTGTTAAAA TTGTGCGCAG CTATGACAAC 
CACGCCAAAT GCGGTCTGGT ATGGGTACCG CATGTGGTGG CCTACAGGTG CCGCACTTGC 
GGCATATCGC CCTGCATGTC CATATGCCGG GACTGCTTCA AGAAGGGCAA CCACACGAAC 
CACGACTTCA ATATGTTCCT GTCACAGGCG GGCGGTGCCT GCGATTGTGG TGACACCTCG 
GTCATGAAGG CGGAGGGTTT TTGCAGTGAT CATGGCATCA ATAATCGGGT GAATCGCGAT 
CCGGTGCCAA ACAATCTTTT GGCAGTGGCC GAGGCCATAA TGCCCAAGCT GCTCTTCCGC 
CTGCTGCAGC ACTTCCGTGA GCACAGCGAC ACCCCACTGG AGGTGCAAGC GATAACGTCG 
TACTCCTGCG AGGAGTTCGC CAACATGCTG ATCGATCTGA ACAACATGGG CGAAATAATG 
CGCAAAGTGA TGACGCGCAC GCTGATAAAT CCGGAGGTGT ATGCCTTCTT CATGGAAGCA 
CCGTGCCAGG ACACCCGGAA TGGACGCTTC TTGAAGGCGA ACCGAGAGAA GTACGAGGAT 
GCCGTCAATA GATTCCCAAA CCCAGAGCCA CCCGATGAGT ACAGAGATCT GCCCGCGCTG 
GGCGACAAAT TGGTCCACAC CACGCTACTC GAAGAGTTCA TCTTCTGGAC ATTTAAGTTC 
GAGTTTCCAC AGACTCTGGT CTGCTTTCTG CTGAACATGC TGCCTGATCA GGATTACAAG 
GAACACCTCA CCCGCACATT TGTGATGCAC TACAGCCGCA TACCGTCCGT GTTGGAAATG 
TCCCGTGATC CGGACACGCT CAGCAACCGG GTGGTCCACA TGAGCGTCCA ACTCTTCTCT 
AACGAAAGTC TGGCCCTCAA GATGGTCAAC GAGCTGTCGC TGCTGCACGT AATGATTATT 
AGTCTGAAGC TAATGATGTC CAAGATACTC ATACAGAATA CATTGCATGA TCCCAACAAG 
AATTTTCATT TCGTCATCGA CTGCACGCGT CAGGTGATGA AGGACCACTG CTACTGGCCA 
CTAGTCTCGG ACTTTAACAA CGTCCTTTCA CACGAATCGG TGGCCCTGGT CTTTCTGCGG 
GACGACAATC TCATCGATAT GTGGTTCCAG TTTCTCCAGA TGCTCCAGGG CATGAATGTC 
AATGTGCGCG AGACGGCTTC TCATGTGGAA TTCGAGCCAA ATAGCTACTA TGCGGCGTTC 
TCCTGTGAGC TTGAGGCCAG TGCCTATCCC ATGTGGTCGA TTATCTCGCA TCTGCAGGAT 
GGCACACATG CCCATCTGGC AAAGAAGATC ATCAACTACT GTGTGACGAC GCTGCACGAG 
TGGCTAGACT CTATTTACTT TATGGAGGCG CGTCTATCTA TGGAGGAGAT GATGCAGGCT 
TCGTTCCACT TTCCCCTGCA TCGTTATCTG GCTGCCTTTG TTTGCCAGGC GGTAACTAAA 
ATGGGAATCA GTCTGAACGA TGTGCTGCCC TCACGCCCCT ATCTGCTGCC CCTGCTGATG 
ATCCATCCAC TTCGCGTCCA GAGTTTTTTC TACGAGATCC TGGCCGGAAA ATGGGTGCGC 
AATGGTCTCC AAATCAAGGG ACAAGCGATG ACCTATATCC AGGCGAACTT CTGCAACTCG 
ATGGCCGACA TGGACCTGTT CTTCCTGCAA ATCTGTGCCA CCAACCTGCC GCAGTACTTC 
TTTCTGCAGA ACACCATCGA GTTGTTCGAC GTAGGTCAAT GGCTGGAGAC AGCTCCCTTG 
AAGCAGCCTC AAAAGGCGGA GCAATCCTCG ATGCTGGAGG GATTCCTAAC CTTCTTGGCC 
ACCCTGGTGA CGAGTCGCAC TAATCTGGGA AACGATGAAG CCACCCAATG CATTATTGAG 
ATCAGTGCCT TGTTGGCTAC CGAGAACAAG ACGCATTCGC AGCTCCTTGA ACTAATGCCG 
GAGCGGTCGG GCAATGTGCA TACCAAAAAC TTTGAGACCT TCCTTAAGAA GCTATCGGTT 
TACAAGGCTC CGTCGAGTGG GAGCGAGAAC CTGGAGCAGG GTCTCTTCAC GCCCATCGAC 
GAGGTGTGGG AGAAGCACTA CGATCCGCTG CACGTCCTTT TGCGAGCAGT TCATCGTCGA 
GACTTTCAAT CATCGCTGGA CCGCTTTACA AACTATGTGA AGAGCAAGGA TAAGATGCCG 
GCAAGTGGTA ACCTATGGCC ACCCTTCCGA TTGCCTCATG CTCTGCCCGC AACGAGCTCC 
TTCAGTGATC CCTGCAAGAT TTTGAACTCG CGCATCTTTC ACTCCACCAT CCTGTCGATA 
TTTTTCCGTG CAGTTCACAC ACGTGATGTT TCCGAGCATC TGCTGGCTCT GGCTGTGTTC 
CTCTTAGAGA TTGCTGTGGA AACAAGTGAT GATGTTGGCA GTGGAACTGG AGATCGGGCC 
CCTCCTGCGC TGGCTGCTGT GGTGGAGTCA TCTAGCGGGC CGGGCTATCA TGGCTACGGC 
ACGGGACGGC ATGAGCCGCC CAAGCTTTTC CACTGCTATC CCACGGACAA TCTAAGCTGC 
AATCTGCGCC ACGTGGTCAA GAAAGTGTCG CTGAAGTCAC GCGATCCGCA AGTGATCACC 
TCCAGCTATC GGTCGAACCC ATTTTACTCA GATCTGGACT TTGAGGTTGA AGCGGATCCA 
GAGCAATCGA TGCGCATGAT TGGTCAAGGG GACCCCGAAG GAGATGAGGC AACGGGTGGA 
GCTGGACTGG GAATGGGAGC TCATAGCAGA AGGAATGTCT CTCAGGCGCT GGTGCCGATG 
AGAGTGCCCG GCATGGAGGT CGCCCTACCA CCGGATCTTT CAGTAGTTGC CGAAACCGGT 
GTGGTTATCC GCCAGGATAG CAACGAAGAT GATCTACTGA GAGAAGGCAA TCATGCCATG 
GATATGAGTC CACCGGCTGC TCTCGATTTC CACTTTCCGC TGCAGCAGAT CACTTTGCCG 
GAGAGCGGAA TGGAGGTGGC TATTCGGAGA GATCTTCTGC TAGCCGAAAC GAATAATATG 
GGCGCAATTG CAGGTGGAGC TGGAGGTGGC GCCAATGCAT CGGCCACACC ACCAGCAGGG 
GCTTCCAATG AGATGTTCTC GCCTACCACA CCCACAGGCA GCGGTATGCT CCTGCCTTTC 
CAGCGGGTTC AGCCTGTAGC CGTACCCAGT AGCGGTAATA TGGATATTGT ACCATCAAAT 
GCCATGGGTG CCGGCGGAAG TTTCACATCC GGCGTAAGTG GTAGCGGCAC TGGACGTAGA 
ATGAACTACG AAACGGGTGG AGCACGTAAG CGATCCGTGG ACATTGCCAT CGGTGGCAGC 
AATAAGGATG AGCTGCATTT GGACGAAAGC ATTTTGTCCC TGCTACTAAA GCTTCATTCT 
CAGCTCTCAG GCACACTAGA TAGCTTCTCG TTGAGCGACG GTGAAGATCA ATCATCTGAC 
GACGATTCGA CCATGGATGT GGACTGTAAC GAAGCCAGCA CCTCCATGGC AGCAGCGGAG 
AGCACTGCCC TGGCTGAGAG AGGACGCAGT AAGCGCAACT ACAAGAACAT TCATGTATCC 
TCCTCGCGCA TCGGTGACGG ACCATTCTTT ATTGGTAATC TGCTGCGAAA GATCGCCAAG 
CAGGATGAGC AGTGCGCCCA GAGCATCGAC GACATCCGTG CCAGACTCTG GCCGAATCAG 
CGTGAAAAAC AAGCAGAGGC GAAAGCACGC GAGGCCAAGG AGAAGGAGGA GCGCCGCAAA 
AAAGCACGTG AGCGCCAGCA GAAGATGATG CAAGACTTCG CCAACAAGCA GAAGCTCTTC 
ATGCAATCCG CTGCAGCGTC ATCCAGTGGC ATGGGCTACG GACCGGAGGA CGAGGACGAT 
GAGGAGTTGT ACGAGGAGCA GCCGCGTGAG AAAGAGTACG ATTGCATAAT CTGCAATTGC 
ACTACGCCCA GTACCGAATC GAATCCTATC GGCTTGGTCG TTCTGGTCGA GTCCAGCGGC 
ATTGTTGGTC ATCGGCGGCG CATCGCCGAG CGATTGCCGC TGCCGATAAA CGCGGAGGAT 
GAGTCCCGAC TGGCGCACAC CACTCGACTG GCGGCTGAAT TCAACAGGCG CACGGAACTG 
CTCAGTCTGA AGTTTGGCGA TGAGTCCTGG TATCTGTCCA ATAATATGGC CTACGACAAC 
GGTGTACATG TGCAGTCGTG TGGCCACCAC GTTCATCTCA GTTGCCTAGA GGCATACCTT 
AAGACGCTGT ACACCACCCA ACGTCAGCCA GTTCAGGATC GTGGCGAGTT CTACTGTCCC 
GTGTGCCGAC AGCTCTCAAA CTCGGTGCTG CCGCTGAGTC CACAACTGGA CAGACCCACG 
CACCTGGTCC GCTCTGGTAA TCAGCCATTC GAACGTCTGG TCGCCGATCT AACAGATCTG 
ATTAAAGAGA ACGAAACGAT ACCGCCAACA AAACTGACAG AGGCCATGGG CCATGCCATG 
GAGGTGATGA CAAACATTGC CCAGCGCAAG GTTAAATGCT CCTCGATAAC GTTCCGCAAG 
CTGTTCATTT TCGTTACCTC GATTGCGCGT ACAAACCTGG AGGCGGAGAT CATCCAGCGG 
GGCGGATCAC TATGTACAGC GAATGCCACG CGGTACAAGC CAAAGCGGGA ATGCATCGTA 
CCGCTACTGC ACGTGCTATC GGTACATGTG CGTGTCTTGG TTGAATGGCC GCTGTGGAGC 
AGCTGGGCCT CGTTAGCTGG CCTGCCCGTT ACCGCTACGG AACCGTTGCC GGCGCACTGC 
CTCGAATTGA TTCCCAGCAT ATTAGCGGAT CCCATTGCTC TGCTGCTCAA GTTTATCCTG 
CTGGCGCCAC TGCAACTCGA TCAGGATTAC TTTACGTGCA TGGTGAAAGT CATGTACAAT 
CTGCTGTACT ATCAAATTGT GGTTCAGCTG TGCGTTACTC TCACCGATTT AGAGTGCGAT 
CACATTGTCA AAGTTTACGG CAGCACTAGC GTTGGCAGTG ACAACTCTGC TGCGGAATCC 
CAACAGCAGG AGTCTGCCGC CGGGACAACG AACAACCGTC GCCGTGCTGG ACAACAGCAG 
CAGAGCTCCT CGCAGCTAGG AAAGGCCATG GCCCTGGTGC TGAGCCAGAC AAATGACCTA 
GTTCACTTGC GACGCGACTG CATACCCAGT ACCAGCAGCT CGGCGGCAGC GTCGGCGGCT 
GGTTCCTCCT CCACAACGTC CACAAACCAT GGAGCTAGCG CAGCCACAGC CTCCTCTGCC 
ACGACGATCG AGGTTAATCT GAAGTCCATG GAATTACAGC TGCAGGCGCT ATGCTTGCCG 
TTTTTGCGAG TAGCTGCCCT GCTGCGCCAG CATCTCTATC GCCATGAGAT GCCGGAGATC 
TCGGCACCGG GACTGGAATT TGTGCGCCTT GTTTACTATT TGGAACTCGT AACAGATTCT 
ATGGACTGGG ATTGCTTTAA TGCCAGCAAG GGGCTATGCT TTATTCCCGG CACTGAGACA 
ACGCTGCCCC AGTTCTGGTG CCAGCAGTTG ATGGAGGTGC GTCCGCCGGC CGATACGGTC 
AGAGAATTGG TGCTCATCAA TCAGCATTCG TTGTGGCAGC AGCCGCGACT TCTTGAATTG 
CCGCGCGAGT ACGAACGCTT GTTCACGTAC TACCATGAGC GGCCGTGCCT CAATTGCTAT 
AAAGTGCCCA AGGAGAGCTC CATCTGCCTG CTGTGCGGGA CGATCGTATG CCTCAAGCAG 
AACTGCTGCG CGGAGAATGA CTGCTGCGAG GCTGTTCGCC ATACGCTATC CTGTGGCGGT 
GGCATAGGCA TCTTTTTGGT GGTCACCTCC ACGTACATCA TTGTTATCCG CGGACGACGC 
GCCTGCCTTT GGGGCTCACT CTATCTGGAC GACTTCGATG AGGAGGATCG CGATCTCAAG 
CGCGGCAAAC CTTTGTATTT GAGCAAAGAC CGCTTCAATC TCCTGGAGTC GCAGTGGCTG 
TCCCATAAGT TTGCTCATAC AAAACACACA TGGGTGTTTC ACCGGGATCT CTTGTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

PubMed

book

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,


book

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,


book

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE


book

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH


book

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D