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Wdr59 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Summary

Gene Symbol Wdr59
Entrez Gene ID 34540
Full Name WD repeat domain 59
Synonyms CG4705,Dmel\CG4705,FBtr0080196,SEA3,Sea3
General protein information
Preferred Names

WD repeat domain 59

Names

CG4705 gene product from transcript CG4705-RD
CG4705-PB
CG4705-PD
Wdr59-PB
Wdr59-PD
Gene Type protein-coding
Organism Drosophila melanogaster(fruit fly)
Genome

2L

32D2-32D3

Reference Sequences (RefSeq)

mRNA and Protein(s)

mRNA Protein Name
NM_205960.4 NP_995682.1 WD repeat domain 59, isoform B
NM_135641.4 NP_609485.4 WD repeat domain 59, isoform D

Pathways from BioSystems


Homologies


Homo sapiens (human) WDR59 NP_085058.3
Saccharomyces cerevisiae (baker's yeast) MTC5 NP_010413.3
Anopheles gambiae (African malaria mosquito) AgaP_AGAP008331 XP_317129.4
Kluyveromyces lactis KLLA0F19734g XP_455966.1
WDR59 XP_001109464.1
Rattus norvegicus (Norway rat) Wdr59 XP_006255704.1
Gallus gallus (chicken) WDR59 NP_001012794.1
Schizosaccharomyces pombe (fission yeast) SPAC11E3.05 NP_594930.1
Bos taurus (cattle) WDR59 NP_001094600.1
Xenopus tropicalis (tropical clawed frog) wdr59 XP_004914115.1
Pan troglodytes (chimpanzee) WDR59 XP_511107.2
Canis lupus familiaris (dog) WDR59 XP_536785.3
Mus musculus (house mouse) Wdr59 NP_001164214.1
Drosophila melanogaster (fruit fly) CG4705 NP_995682.1
Eremothecium gossypii AGOS_AGR067W NP_986732.2

Gene Ontology


Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Wdr59 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Wdr59 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OFa16652 NM_135641.4
Latest version!		 Drosophila melanogaster WD repeat domain 59 (Wdr59), transcript variant D, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 12-14 $510.30
$729.00
OFa16652 NM_205960.4
Latest version!		 Drosophila melanogaster WD repeat domain 59 (Wdr59), transcript variant B, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 12-14 $510.30
$729.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone 30% OFF
2-4 Clone 40% OFF
5 or more Clone 50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)


CloneID OFa16652
Clone ID Related Accession (Same CDS sequence) NM_205960.4 , NM_135641.4
Accession Version NM_205960.4 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 2910bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2019-04-24
Organism Drosophila melanogaster(fruit fly)
Product WD repeat domain 59, isoform B
Comment Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). On Jul 15, 2014 this sequence version replaced NM_205960.3. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END## 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
ATGCCGCCCA CGGAGACACT GCGTCCGGGA GAACGCGGAA CAGCCGGTGG ACCGGGAGCA 
GGAGCACCCG AACAAACGTA CATCGTCAGG CAGAGCAACA AATACTATGA ACACCGAGAT 
TCCCAGGCCA CGGCCATGTC GGTTGATTAC TCGGGTCAGT GGGTGCTCCT CGCGGGCCGT 
GGTCACCTGG CTTTGCAGCG TTTGGGACAG GACGATGGGT CTCTGCGACG ACATGAACGG 
CAATCGAAGT ACGAGGTGTC CGTAGCAGAG TTTGCCATAT GTCCCAGTCG CAAAGAGTAC 
TGCGCCATAG CGACTAGCCA GCACATAGAC ATCGTTCGCT GGGGCACTGC GGAACCACAC 
TACGAGATGT CCTTGCGTGG GCATACTCGA ACAGTGACCG ACATTGACTG GCACGGCAAG 
GATCCGAATC TGCTGGTCAG CTGCTCAATA GACACATTTT CTCATATTTG GGACTTAAGG 
GAGCCCAGGA AACCAGCCCT GTCCTTGAAT GCCGTGTGCA TGTCTGGTGC TACACAAGTG 
GGATTCAACC GCGTTTCGGG CAACCTTTTG GCTGCTGCCC ATGATGGAGA TTTACGCATC 
TGGGATATTC GCAAGGGTAG TTGCCCGACG CACTATATCA CAGCACATTT AAATAGAGTG 
CATGGCATCA ATTGGAGCCA CAAAAGGGAG ACCTGCTTGG CAACCGCCAG TCAGGATGGA 
ACGGTCAAGT ACTTTGATGT ATGTAATCCT CGCAGGGCCG AGAAGATCAT TACAACCATG 
TCGCCCGTTT GGAGAGCGAG ATACACGCCT ATTGGCAACG GTCTGGTTAG CATTGTGGTG 
CCCCATTTGG GACGCGGAGA GAATAGTTTG CTGCTCTGGA GCAACAGCAA GCAAACGGAC 
CCTATTTGTT CCTTCGTGGG GCACACCGAT GTCATTCTTG ACTTTGCCTG GCGTCCAAAT 
CGTGAAAGTT CCAATGAAAT AGAACTGGTC ACTTGGTCCA GGGATCGCAC GCTGCGGGTG 
TGGAAGATCG ATGATAATAT GCTAAAGCTT TGCGAACCGT CTGCGGAAGA AATAGAATCC 
CGTTTCGAGC CCGATCTCAG TGAGTTAAGA GTGCCTACTC CTCCAGAATT CCTGCATCCA 
CGATCAATTT TGGTTGCCGC TTCGTTGCCC ATATCCACGG GTGACGGTGC TTGTAATACC 
TTGCCCATGG CTCGCTCCCC GAGCTTTGGA GGTGGCTACT ATCGCAGGGA AGAGCCCCAC 
ATAGCCAGAT CACTAACAGA TCAACCCACG TGTTCGCTGC ACCACGAATT CTCGCTGCTC 
AACACAAACA TGCCTCATGT GGAGGTAGAC ACACTAGATG CCATAAAGCG GTACGCCTGC 
TTCAAGATTT GCGCCGGTGG ACACACGGTT ATTTTACAAG TGACTTTCAC GACATCGTAT 
CCAAGTCCTA GTGCTCCGCC GGACTTTCAG CTTTGCCAGG GAACCACTCT GTCCAGTGAA 
GTTAGTGGAG TTCTGCTAAA AGTGCTCCGT TGCAATGCAT TGCAACGGGT CAAGAAATCC 
CGCACGTGCC TGGAACAATG CCTACGTGCT CTAGTGGCGG CTATGAAGAA AAAGGTGGCC 
GCTGTAGGCG GAGCGGATCG TAGCCAACTG CTCCTGCAAT CCCCTCGGCT GGAAGGAGCT 
TTGTCGAGCA CCCTGCACGA TGCCTGCATT CCATATCCGC GGACGTCAGG GGTCCATTTC 
AATGCCATTG GTATGCTGAC CACTTTCGCC CAACCAGTGA ACAACAAAAG GCTGACCCTG 
CGGCAACACA CCGCATTGAC TCCACGGACT TTTTCATCGA TTAATGGTAG TGGATTGCTA 
GGCAATGTGA TGGCCACCGC CCAGAGGGAA GCTAACGCCT CCTTTTACCT CCAGGAGAGG 
ATGATTTCGG GAAAACCGGG CAAGCAGCGA GCCATCCGAC AGATGAATGG AAGTCCGGTG 
GTTCACGTAT ACGACACAAG TAGCTTACTC CACATTAGTC GGAGAATGGC TCGGGAGTTC 
TCCCTCGATA AGTGCAACAT AGCAGAAACG TGTCGTAAAA ATGGTGAGAT CTGTCGCCAG 
CATGGGCGCT TGGACTTGGT GCCCGTGTGG CTATTGGCGG AACTGATAGC CACACCCCAA 
ATTCCACATG AAACACTCAA CGATCTGCTG TTCTACAAAG ATCCCTTCAA GAAATCTCTG 
CTGGAATCAC TAATAATGCA TTATGCTATC TCCGGTGACA TACAGACTGC TGTGCTGCTG 
GCTTGTTTGT TTGACAAGTG CCCCACGGGT GGCGGTGCCA TTATGGAGAA GACAGTTCGA 
CTTCCGCCGC AGCTTAACTC GCAAATTTCA CCGTATCACA CGGTCCTGCC GCTTGACGTC 
AAGTCATCTT CTTCATCGAA CACTTGGCAG CAACTTAAGC AACTACGCAG CAACTCTTGG 
TCCGACTCCC TGGATTTGGA GATTAAGCAA ATCCAGTCTG ATGCATACGC TTGCTCATTG 
ATTAGACGGA CTAAGATGCC ACTGTTTGAT CAATTTAAGC GCGCCTATGC CGAAATTTTA 
TTTGGCTGGC AGTTGCTTTC GAAGCGGGCT TTGATCCTCA AGCACACGCA GAATACACCA 
CCTCCAGTCC AGGGTGTGGA ATTCGTTACC GAGTGCAGGA AGTGTGCCAA GCCCAAACGG 
ACGCCCAAAT GCGAGCCCTG CAAGCGACCC GTACTCTTCT GCGTCCTCTG CCGTTTGCCT 
GTGAAAGGAG CAGCCAATGC GTGTCTGGCT TGTGGCCATG GCGGTCACAT TGATCACATG 
ATGCAGTGGT TTGAGAAACA CAATGTCTGT GCTACGTGTG GCTGCAAGTG CTTGGAGCGC 
ACCTCAGAGC TGCTAGCCCT ACTCAGCTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_995682.1
CDS124..3033
Translation

Target ORF information:

RefSeq Version NM_205960.4
Organism Drosophila melanogaster(fruit fly)
Definition Drosophila melanogaster WD repeat domain 59 (Wdr59), transcript variant B, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 NM_205960.4

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
ATGCCGCCCA CGGAGACACT GCGTCCGGGA GAACGCGGAA CAGCCGGTGG ACCGGGAGCA 
GGAGCACCCG AACAAACGTA CATCGTCAGG CAGAGCAACA AATACTATGA ACACCGAGAT 
TCCCAGGCCA CGGCCATGTC GGTTGATTAC TCGGGTCAGT GGGTGCTCCT CGCGGGCCGT 
GGTCACCTGG CTTTGCAGCG TTTGGGACAG GACGATGGGT CTCTGCGACG ACATGAACGG 
CAATCGAAGT ACGAGGTGTC CGTAGCAGAG TTTGCCATAT GTCCCAGTCG CAAAGAGTAC 
TGCGCCATAG CGACTAGCCA GCACATAGAC ATCGTTCGCT GGGGCACTGC GGAACCACAC 
TACGAGATGT CCTTGCGTGG GCATACTCGA ACAGTGACCG ACATTGACTG GCACGGCAAG 
GATCCGAATC TGCTGGTCAG CTGCTCAATA GACACATTTT CTCATATTTG GGACTTAAGG 
GAGCCCAGGA AACCAGCCCT GTCCTTGAAT GCCGTGTGCA TGTCTGGTGC TACACAAGTG 
GGATTCAACC GCGTTTCGGG CAACCTTTTG GCTGCTGCCC ATGATGGAGA TTTACGCATC 
TGGGATATTC GCAAGGGTAG TTGCCCGACG CACTATATCA CAGCACATTT AAATAGAGTG 
CATGGCATCA ATTGGAGCCA CAAAAGGGAG ACCTGCTTGG CAACCGCCAG TCAGGATGGA 
ACGGTCAAGT ACTTTGATGT ATGTAATCCT CGCAGGGCCG AGAAGATCAT TACAACCATG 
TCGCCCGTTT GGAGAGCGAG ATACACGCCT ATTGGCAACG GTCTGGTTAG CATTGTGGTG 
CCCCATTTGG GACGCGGAGA GAATAGTTTG CTGCTCTGGA GCAACAGCAA GCAAACGGAC 
CCTATTTGTT CCTTCGTGGG GCACACCGAT GTCATTCTTG ACTTTGCCTG GCGTCCAAAT 
CGTGAAAGTT CCAATGAAAT AGAACTGGTC ACTTGGTCCA GGGATCGCAC GCTGCGGGTG 
TGGAAGATCG ATGATAATAT GCTAAAGCTT TGCGAACCGT CTGCGGAAGA AATAGAATCC 
CGTTTCGAGC CCGATCTCAG TGAGTTAAGA GTGCCTACTC CTCCAGAATT CCTGCATCCA 
CGATCAATTT TGGTTGCCGC TTCGTTGCCC ATATCCACGG GTGACGGTGC TTGTAATACC 
TTGCCCATGG CTCGCTCCCC GAGCTTTGGA GGTGGCTACT ATCGCAGGGA AGAGCCCCAC 
ATAGCCAGAT CACTAACAGA TCAACCCACG TGTTCGCTGC ACCACGAATT CTCGCTGCTC 
AACACAAACA TGCCTCATGT GGAGGTAGAC ACACTAGATG CCATAAAGCG GTACGCCTGC 
TTCAAGATTT GCGCCGGTGG ACACACGGTT ATTTTACAAG TGACTTTCAC GACATCGTAT 
CCAAGTCCTA GTGCTCCGCC GGACTTTCAG CTTTGCCAGG GAACCACTCT GTCCAGTGAA 
GTTAGTGGAG TTCTGCTAAA AGTGCTCCGT TGCAATGCAT TGCAACGGGT CAAGAAATCC 
CGCACGTGCC TGGAACAATG CCTACGTGCT CTAGTGGCGG CTATGAAGAA AAAGGTGGCC 
GCTGTAGGCG GAGCGGATCG TAGCCAACTG CTCCTGCAAT CCCCTCGGCT GGAAGGAGCT 
TTGTCGAGCA CCCTGCACGA TGCCTGCATT CCATATCCGC GGACGTCAGG GGTCCATTTC 
AATGCCATTG GTATGCTGAC CACTTTCGCC CAACCAGTGA ACAACAAAAG GCTGACCCTG 
CGGCAACACA CCGCATTGAC TCCACGGACT TTTTCATCGA TTAATGGTAG TGGATTGCTA 
GGCAATGTGA TGGCCACCGC CCAGAGGGAA GCTAACGCCT CCTTTTACCT CCAGGAGAGG 
ATGATTTCGG GAAAACCGGG CAAGCAGCGA GCCATCCGAC AGATGAATGG AAGTCCGGTG 
GTTCACGTAT ACGACACAAG TAGCTTACTC CACATTAGTC GGAGAATGGC TCGGGAGTTC 
TCCCTCGATA AGTGCAACAT AGCAGAAACG TGTCGTAAAA ATGGTGAGAT CTGTCGCCAG 
CATGGGCGCT TGGACTTGGT GCCCGTGTGG CTATTGGCGG AACTGATAGC CACACCCCAA 
ATTCCACATG AAACACTCAA CGATCTGCTG TTCTACAAAG ATCCCTTCAA GAAATCTCTG 
CTGGAATCAC TAATAATGCA TTATGCTATC TCCGGTGACA TACAGACTGC TGTGCTGCTG 
GCTTGTTTGT TTGACAAGTG CCCCACGGGT GGCGGTGCCA TTATGGAGAA GACAGTTCGA 
CTTCCGCCGC AGCTTAACTC GCAAATTTCA CCGTATCACA CGGTCCTGCC GCTTGACGTC 
AAGTCATCTT CTTCATCGAA CACTTGGCAG CAACTTAAGC AACTACGCAG CAACTCTTGG 
TCCGACTCCC TGGATTTGGA GATTAAGCAA ATCCAGTCTG ATGCATACGC TTGCTCATTG 
ATTAGACGGA CTAAGATGCC ACTGTTTGAT CAATTTAAGC GCGCCTATGC CGAAATTTTA 
TTTGGCTGGC AGTTGCTTTC GAAGCGGGCT TTGATCCTCA AGCACACGCA GAATACACCA 
CCTCCAGTCC AGGGTGTGGA ATTCGTTACC GAGTGCAGGA AGTGTGCCAA GCCCAAACGG 
ACGCCCAAAT GCGAGCCCTG CAAGCGACCC GTACTCTTCT GCGTCCTCTG CCGTTTGCCT 
GTGAAAGGAG CAGCCAATGC GTGTCTGGCT TGTGGCCATG GCGGTCACAT TGATCACATG 
ATGCAGTGGT TTGAGAAACA CAATGTCTGT GCTACGTGTG GCTGCAAGTG CTTGGAGCGC 
ACCTCAGAGC TGCTAGCCCT ACTCAGCTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OFa16652
Clone ID Related Accession (Same CDS sequence) NM_205960.4 , NM_135641.4
Accession Version NM_135641.4 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 2910bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2019-04-24
Organism Drosophila melanogaster(fruit fly)
Product WD repeat domain 59, isoform D
Comment Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). On Jul 15, 2014 this sequence version replaced NM_135641.3. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END## 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
ATGCCGCCCA CGGAGACACT GCGTCCGGGA GAACGCGGAA CAGCCGGTGG ACCGGGAGCA 
GGAGCACCCG AACAAACGTA CATCGTCAGG CAGAGCAACA AATACTATGA ACACCGAGAT 
TCCCAGGCCA CGGCCATGTC GGTTGATTAC TCGGGTCAGT GGGTGCTCCT CGCGGGCCGT 
GGTCACCTGG CTTTGCAGCG TTTGGGACAG GACGATGGGT CTCTGCGACG ACATGAACGG 
CAATCGAAGT ACGAGGTGTC CGTAGCAGAG TTTGCCATAT GTCCCAGTCG CAAAGAGTAC 
TGCGCCATAG CGACTAGCCA GCACATAGAC ATCGTTCGCT GGGGCACTGC GGAACCACAC 
TACGAGATGT CCTTGCGTGG GCATACTCGA ACAGTGACCG ACATTGACTG GCACGGCAAG 
GATCCGAATC TGCTGGTCAG CTGCTCAATA GACACATTTT CTCATATTTG GGACTTAAGG 
GAGCCCAGGA AACCAGCCCT GTCCTTGAAT GCCGTGTGCA TGTCTGGTGC TACACAAGTG 
GGATTCAACC GCGTTTCGGG CAACCTTTTG GCTGCTGCCC ATGATGGAGA TTTACGCATC 
TGGGATATTC GCAAGGGTAG TTGCCCGACG CACTATATCA CAGCACATTT AAATAGAGTG 
CATGGCATCA ATTGGAGCCA CAAAAGGGAG ACCTGCTTGG CAACCGCCAG TCAGGATGGA 
ACGGTCAAGT ACTTTGATGT ATGTAATCCT CGCAGGGCCG AGAAGATCAT TACAACCATG 
TCGCCCGTTT GGAGAGCGAG ATACACGCCT ATTGGCAACG GTCTGGTTAG CATTGTGGTG 
CCCCATTTGG GACGCGGAGA GAATAGTTTG CTGCTCTGGA GCAACAGCAA GCAAACGGAC 
CCTATTTGTT CCTTCGTGGG GCACACCGAT GTCATTCTTG ACTTTGCCTG GCGTCCAAAT 
CGTGAAAGTT CCAATGAAAT AGAACTGGTC ACTTGGTCCA GGGATCGCAC GCTGCGGGTG 
TGGAAGATCG ATGATAATAT GCTAAAGCTT TGCGAACCGT CTGCGGAAGA AATAGAATCC 
CGTTTCGAGC CCGATCTCAG TGAGTTAAGA GTGCCTACTC CTCCAGAATT CCTGCATCCA 
CGATCAATTT TGGTTGCCGC TTCGTTGCCC ATATCCACGG GTGACGGTGC TTGTAATACC 
TTGCCCATGG CTCGCTCCCC GAGCTTTGGA GGTGGCTACT ATCGCAGGGA AGAGCCCCAC 
ATAGCCAGAT CACTAACAGA TCAACCCACG TGTTCGCTGC ACCACGAATT CTCGCTGCTC 
AACACAAACA TGCCTCATGT GGAGGTAGAC ACACTAGATG CCATAAAGCG GTACGCCTGC 
TTCAAGATTT GCGCCGGTGG ACACACGGTT ATTTTACAAG TGACTTTCAC GACATCGTAT 
CCAAGTCCTA GTGCTCCGCC GGACTTTCAG CTTTGCCAGG GAACCACTCT GTCCAGTGAA 
GTTAGTGGAG TTCTGCTAAA AGTGCTCCGT TGCAATGCAT TGCAACGGGT CAAGAAATCC 
CGCACGTGCC TGGAACAATG CCTACGTGCT CTAGTGGCGG CTATGAAGAA AAAGGTGGCC 
GCTGTAGGCG GAGCGGATCG TAGCCAACTG CTCCTGCAAT CCCCTCGGCT GGAAGGAGCT 
TTGTCGAGCA CCCTGCACGA TGCCTGCATT CCATATCCGC GGACGTCAGG GGTCCATTTC 
AATGCCATTG GTATGCTGAC CACTTTCGCC CAACCAGTGA ACAACAAAAG GCTGACCCTG 
CGGCAACACA CCGCATTGAC TCCACGGACT TTTTCATCGA TTAATGGTAG TGGATTGCTA 
GGCAATGTGA TGGCCACCGC CCAGAGGGAA GCTAACGCCT CCTTTTACCT CCAGGAGAGG 
ATGATTTCGG GAAAACCGGG CAAGCAGCGA GCCATCCGAC AGATGAATGG AAGTCCGGTG 
GTTCACGTAT ACGACACAAG TAGCTTACTC CACATTAGTC GGAGAATGGC TCGGGAGTTC 
TCCCTCGATA AGTGCAACAT AGCAGAAACG TGTCGTAAAA ATGGTGAGAT CTGTCGCCAG 
CATGGGCGCT TGGACTTGGT GCCCGTGTGG CTATTGGCGG AACTGATAGC CACACCCCAA 
ATTCCACATG AAACACTCAA CGATCTGCTG TTCTACAAAG ATCCCTTCAA GAAATCTCTG 
CTGGAATCAC TAATAATGCA TTATGCTATC TCCGGTGACA TACAGACTGC TGTGCTGCTG 
GCTTGTTTGT TTGACAAGTG CCCCACGGGT GGCGGTGCCA TTATGGAGAA GACAGTTCGA 
CTTCCGCCGC AGCTTAACTC GCAAATTTCA CCGTATCACA CGGTCCTGCC GCTTGACGTC 
AAGTCATCTT CTTCATCGAA CACTTGGCAG CAACTTAAGC AACTACGCAG CAACTCTTGG 
TCCGACTCCC TGGATTTGGA GATTAAGCAA ATCCAGTCTG ATGCATACGC TTGCTCATTG 
ATTAGACGGA CTAAGATGCC ACTGTTTGAT CAATTTAAGC GCGCCTATGC CGAAATTTTA 
TTTGGCTGGC AGTTGCTTTC GAAGCGGGCT TTGATCCTCA AGCACACGCA GAATACACCA 
CCTCCAGTCC AGGGTGTGGA ATTCGTTACC GAGTGCAGGA AGTGTGCCAA GCCCAAACGG 
ACGCCCAAAT GCGAGCCCTG CAAGCGACCC GTACTCTTCT GCGTCCTCTG CCGTTTGCCT 
GTGAAAGGAG CAGCCAATGC GTGTCTGGCT TGTGGCCATG GCGGTCACAT TGATCACATG 
ATGCAGTGGT TTGAGAAACA CAATGTCTGT GCTACGTGTG GCTGCAAGTG CTTGGAGCGC 
ACCTCAGAGC TGCTAGCCCT ACTCAGCTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_609485.4
CDS124..3033
Translation

Target ORF information:

RefSeq Version NM_135641.4
Organism Drosophila melanogaster(fruit fly)
Definition Drosophila melanogaster WD repeat domain 59 (Wdr59), transcript variant D, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 NM_135641.4

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
ATGCCGCCCA CGGAGACACT GCGTCCGGGA GAACGCGGAA CAGCCGGTGG ACCGGGAGCA 
GGAGCACCCG AACAAACGTA CATCGTCAGG CAGAGCAACA AATACTATGA ACACCGAGAT 
TCCCAGGCCA CGGCCATGTC GGTTGATTAC TCGGGTCAGT GGGTGCTCCT CGCGGGCCGT 
GGTCACCTGG CTTTGCAGCG TTTGGGACAG GACGATGGGT CTCTGCGACG ACATGAACGG 
CAATCGAAGT ACGAGGTGTC CGTAGCAGAG TTTGCCATAT GTCCCAGTCG CAAAGAGTAC 
TGCGCCATAG CGACTAGCCA GCACATAGAC ATCGTTCGCT GGGGCACTGC GGAACCACAC 
TACGAGATGT CCTTGCGTGG GCATACTCGA ACAGTGACCG ACATTGACTG GCACGGCAAG 
GATCCGAATC TGCTGGTCAG CTGCTCAATA GACACATTTT CTCATATTTG GGACTTAAGG 
GAGCCCAGGA AACCAGCCCT GTCCTTGAAT GCCGTGTGCA TGTCTGGTGC TACACAAGTG 
GGATTCAACC GCGTTTCGGG CAACCTTTTG GCTGCTGCCC ATGATGGAGA TTTACGCATC 
TGGGATATTC GCAAGGGTAG TTGCCCGACG CACTATATCA CAGCACATTT AAATAGAGTG 
CATGGCATCA ATTGGAGCCA CAAAAGGGAG ACCTGCTTGG CAACCGCCAG TCAGGATGGA 
ACGGTCAAGT ACTTTGATGT ATGTAATCCT CGCAGGGCCG AGAAGATCAT TACAACCATG 
TCGCCCGTTT GGAGAGCGAG ATACACGCCT ATTGGCAACG GTCTGGTTAG CATTGTGGTG 
CCCCATTTGG GACGCGGAGA GAATAGTTTG CTGCTCTGGA GCAACAGCAA GCAAACGGAC 
CCTATTTGTT CCTTCGTGGG GCACACCGAT GTCATTCTTG ACTTTGCCTG GCGTCCAAAT 
CGTGAAAGTT CCAATGAAAT AGAACTGGTC ACTTGGTCCA GGGATCGCAC GCTGCGGGTG 
TGGAAGATCG ATGATAATAT GCTAAAGCTT TGCGAACCGT CTGCGGAAGA AATAGAATCC 
CGTTTCGAGC CCGATCTCAG TGAGTTAAGA GTGCCTACTC CTCCAGAATT CCTGCATCCA 
CGATCAATTT TGGTTGCCGC TTCGTTGCCC ATATCCACGG GTGACGGTGC TTGTAATACC 
TTGCCCATGG CTCGCTCCCC GAGCTTTGGA GGTGGCTACT ATCGCAGGGA AGAGCCCCAC 
ATAGCCAGAT CACTAACAGA TCAACCCACG TGTTCGCTGC ACCACGAATT CTCGCTGCTC 
AACACAAACA TGCCTCATGT GGAGGTAGAC ACACTAGATG CCATAAAGCG GTACGCCTGC 
TTCAAGATTT GCGCCGGTGG ACACACGGTT ATTTTACAAG TGACTTTCAC GACATCGTAT 
CCAAGTCCTA GTGCTCCGCC GGACTTTCAG CTTTGCCAGG GAACCACTCT GTCCAGTGAA 
GTTAGTGGAG TTCTGCTAAA AGTGCTCCGT TGCAATGCAT TGCAACGGGT CAAGAAATCC 
CGCACGTGCC TGGAACAATG CCTACGTGCT CTAGTGGCGG CTATGAAGAA AAAGGTGGCC 
GCTGTAGGCG GAGCGGATCG TAGCCAACTG CTCCTGCAAT CCCCTCGGCT GGAAGGAGCT 
TTGTCGAGCA CCCTGCACGA TGCCTGCATT CCATATCCGC GGACGTCAGG GGTCCATTTC 
AATGCCATTG GTATGCTGAC CACTTTCGCC CAACCAGTGA ACAACAAAAG GCTGACCCTG 
CGGCAACACA CCGCATTGAC TCCACGGACT TTTTCATCGA TTAATGGTAG TGGATTGCTA 
GGCAATGTGA TGGCCACCGC CCAGAGGGAA GCTAACGCCT CCTTTTACCT CCAGGAGAGG 
ATGATTTCGG GAAAACCGGG CAAGCAGCGA GCCATCCGAC AGATGAATGG AAGTCCGGTG 
GTTCACGTAT ACGACACAAG TAGCTTACTC CACATTAGTC GGAGAATGGC TCGGGAGTTC 
TCCCTCGATA AGTGCAACAT AGCAGAAACG TGTCGTAAAA ATGGTGAGAT CTGTCGCCAG 
CATGGGCGCT TGGACTTGGT GCCCGTGTGG CTATTGGCGG AACTGATAGC CACACCCCAA 
ATTCCACATG AAACACTCAA CGATCTGCTG TTCTACAAAG ATCCCTTCAA GAAATCTCTG 
CTGGAATCAC TAATAATGCA TTATGCTATC TCCGGTGACA TACAGACTGC TGTGCTGCTG 
GCTTGTTTGT TTGACAAGTG CCCCACGGGT GGCGGTGCCA TTATGGAGAA GACAGTTCGA 
CTTCCGCCGC AGCTTAACTC GCAAATTTCA CCGTATCACA CGGTCCTGCC GCTTGACGTC 
AAGTCATCTT CTTCATCGAA CACTTGGCAG CAACTTAAGC AACTACGCAG CAACTCTTGG 
TCCGACTCCC TGGATTTGGA GATTAAGCAA ATCCAGTCTG ATGCATACGC TTGCTCATTG 
ATTAGACGGA CTAAGATGCC ACTGTTTGAT CAATTTAAGC GCGCCTATGC CGAAATTTTA 
TTTGGCTGGC AGTTGCTTTC GAAGCGGGCT TTGATCCTCA AGCACACGCA GAATACACCA 
CCTCCAGTCC AGGGTGTGGA ATTCGTTACC GAGTGCAGGA AGTGTGCCAA GCCCAAACGG 
ACGCCCAAAT GCGAGCCCTG CAAGCGACCC GTACTCTTCT GCGTCCTCTG CCGTTTGCCT 
GTGAAAGGAG CAGCCAATGC GTGTCTGGCT TGTGGCCATG GCGGTCACAT TGATCACATG 
ATGCAGTGGT TTGAGAAACA CAATGTCTGT GCTACGTGTG GCTGCAAGTG CTTGGAGCGC 
ACCTCAGAGC TGCTAGCCCT ACTCAGCTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

PubMed

book

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,


book

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,


book

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE


book

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH


book

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D