EEF2 cDNA ORF clone, Gallus gallus(chicken)

Gene
Clones
gRNAs

Gene Symbol	EEF2
Entrez Gene ID	396325
Full Name	eukaryotic translation elongation factor 2
General protein information	Preferred Names eukaryotic translation elongation factor 2 Names elongation factor 2 EF-2
Gene Type	protein-coding
Organism	Gallus gallus(chicken)

mRNA and Protein(s)

mRNA Protein Name

NM_205368.1 NP_990699.1 elongation factor 2
Pathways from BioSystems

mRNA	Protein	Name
NM_205368.1	NP_990699.1	elongation factor 2

Homologies

Pan troglodytes (chimpanzee)	LOC100614367	XP_003316060.2
	EEF2	XP_001118006.2
Drosophila melanogaster (fruit fly)	Ef2b	NP_525105.2
Mus musculus (house mouse)	Eef2	NP_031933.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP009441	XP_307854.2
Danio rerio (zebrafish)	eef2b	NP_956752.2
Arabidopsis thaliana (thale cress)	LOS1	NP_849818.1
Oryza sativa (rice)	Os01g0742200	NP_001044211.1
Bos taurus (cattle)	EEF2	NP_001068589.1
Gallus gallus (chicken)	EEF2	NP_990699.1
Caenorhabditis elegans (roundworm)	eef-2	NP_001251010.1
Homo sapiens (human)	EEF2	NP_001952.1
Canis lupus familiaris (dog)	EEF2	XP_533949.3
Rattus norvegicus (Norway rat)	Eef2	NP_058941.1
Xenopus tropicalis (tropical clawed frog)	eef2.1	NP_989255.1

Gene Ontology
Bibliography

Related articles in PubMed

Afferent regulation of chicken auditory brainstem neurons: rapid changes in phosphorylation of elongation factor 2.
McBride EG, Rubel EW, Wang Y
The Journal of comparative neurology521(5)1165-83(2013 Apr)

Regulation of neurite outgrowth mediated by localized phosphorylation of protein translational factor eEF2 in growth cones.
Iketani M, Iizuka A, Sengoku K, Kurihara Y, Nakamura F, Sasaki Y, Sato Y, Yamane M, Matsushita M, Nairn AC, Takamatsu K, Goshima Y, Takei K
Developmental neurobiology73(3)230-46(2013 Mar)

Glutamate-dependent elongation factor-2 phosphorylation in Bergmann glial cells.
Barrera I, Hern?ndez-Kelly LC, Castel?n F, Ortega A
Neurochemistry international52(6)1167-75(2008 May)

Functional characterization of the promoter region of the chicken elongation factor-2 gene.
Lim EJ, Kim CW
Gene386(1-2)183-90(2007 Jan)

Elongation factor-2 in chick embryo is phosphorylated on tyrosine as well as serine and threonine.
Kim YW, Kim CW, Kang KR, Byun SM, Kang YS
Biochemical and biophysical research communications175(2)400-6(1991 Mar)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

This study demonstrated that a reduced level of p-eEF2 is not responsible for immediate responses of a neuron to compromised afferent input but may impair the neuron's ability to initiate recovery signaling for survival.
Title: Afferent regulation of chicken auditory brainstem neurons: rapid changes in phosphorylation of elongation factor 2.

These findings suggest that an eEF2 phosphorylation state localized to the growth cone regulates neurite outgrowth
Title: Regulation of neurite outgrowth mediated by localized phosphorylation of protein translational factor eEF2 in growth cones.

Glutamate receptors regulate eukaryotic elongation factor-2 kinase phosphorylation through the involvement of Ca2+/calmodulin/extracellular-regulated protein kinases 1/2.
Title: Glutamate-dependent elongation factor-2 phosphorylation in Bergmann glial cells.

EF-2 gene promoter is localized to the region -86 and +102 of the 5'-non-coding and upstream flanking region.
Title: Functional characterization of the promoter region of the chicken elongation factor-2 gene.

The following EEF2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the EEF2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OGa26795	NM_205368.1 Latest version!	Gallus gallus eukaryotic translation elongation factor 2 (EEF2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

EEF2 ( NM_205368.1 ) cDNA ORF clone, Gallus gallus(chicken) -> NP_990699.1 Gallus gallus eukaryotic translation elongation factor 2 (EEF2), mRNA.

Price & Availability↑

CloneID	OGa26795
Clone ID Related Accession (Same CDS sequence)	NM_205368.1
Accession Version	NM_205368.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2577bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-22
Organism	Gallus gallus(chicken)
Product	elongation factor 2
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from U46663.1. ##Evidence-Data-START## Transcript exon combination :: U46663.1 [ECO:0000332] ##Evidence-Data-END##

ATGGTGAACT TCACAGTAGA TCAGATACGG GCCATCATGG ACAAAAAGGC CAACATCAGA 
AACATGTCGG TGATCGCCCA CGTTGATCAC GGCAAGTCCA CCCTGACCGA TTCTCTGGTG 
TGCAAAGCTG GGATCATCGC CTCTGCGCGC GCGGGGGAGA CCCGTTTCAC TGACACGAGG 
AAGGACGAGC AGGAACGGTG CATCACCATC AAATCTACAG CTATTTCTCT GTTCTACGAG 
CTCTCGGAGA ACGATCTGGC GTTCATCAAG CAAAGCAAGG ATGGTTCTGG TTTCTTGATC 
AACCTGATCG ACTCTCCTGG GCACGTGGAT TTCTCTTCAG AGGTCACCGC TGCTCTGCGT 
GTCACTGATG GTGCCCTGGT TGTTGTCGAC TGCGTCTCTG GTGTGTGCGT GCAGACAGAA 
ACTGTGCTGC GTCAGGCCAT TGCTGAGAGG ATCAAACCTG TCCTGATGAT GAACAAGATG 
GATCGGGCGC TGCTGGAGCT GCAGCTGGAT CCTGAGGAGC TGTACCAGAC CTTCCAGCGG 
ATTGTGGAGA ACGTGAACGT CATCATCTCC ACGTATGGAG AAGGAGAAAG CGGCCCGATG 
GGAAATATCA TGATCGATCC TGTGCTTGGT ACAGTTGGCT TTGGTTCTGG CCTGCATGGC 
TGGGCTTTCA CTTTGAAACA GTTTGCTGAA ATGTATGTTG CAAAGTTTGC TGCCAAGGGT 
GATGCCCAGA TGAATCCAAC TGAACGTGCC AAGAAAGTAG AAGACATGAT GAAGAAGCTG 
TGGGGAGACA GATACTTTGA TCCTGCTACT GGCAAGTTCA GCAAATCCGC TACTGGCCCC 
GATGGAAAGA AACTGCCTAG GACCTTCTGC CAGCTCATCC TCGATCCCAT CTTCAAGGTG 
TTTGATGCGA TCATGACCTT CAAGAAAGAG GAGGCAGCTA AACTGATCGA GAAACTGGAC 
ATCAAGCTTG ACAGTGAAGA TAAGGACAAG GAGGGCAAAC CCCTGCTGAA GGCTGTGATG 
AGGCGGTGGC TGCCTGCCGG AGATGCCCTG CTCCAGATGA TCACCATTCA CCTGCCTTCT 
CCTGTCACAG CCCAGAAGTA CCGCTGTGAG CTGCTCTATG AGGGGCCCCC TGATGATGAG 
GCCGCCATAG GTATTAAGAA CTGTGATCCA AGGGGATCTC TGATGATGTA CATTTCTAAA 
ATGGTGCCAA CCTCCGACAA GGGACGTTTC TATGCTTTCG GCCGTGTTTT CTCTGGTCTC 
GTATCAACTG GCTTGAAAGT CAGAATCATG GGACCCAACT ACACGCCCGG CAAGAAGGAG 
GATCTGTACC TGAAGCCAAT CCAAAGGACC ATCCTCATGA TGGGCCGCTA CGTTGAACCC 
ATTGAGGACG TGCCTTGTGG AAACATTGTC GGTCTGGTTG GTGTTGATCA GTTCCTGGTG 
AAGACTGGAA CCATCACCAC CTTCGAGCAC GCTCACAACA TGCGGGTCAT GAAGTTCAGC 
GTCAGCCCTG TGGTGCGCGT GGCCGTGGAA GCCAAGAACC CTGCAGACCT GCCCAAGCTG 
GTGGAGGGGC TGAAGCGCCT TGCCAAGTCT GACCCTATGG TGCAGTGCAT CATTGAGGAG 
TCCGGAGAGC ACATCATTGC TGGTGCAGGG GAGCTGCACT TGGAAATCTG CCTGAAGGAT 
CTGGAGGAGG ACCACGCTTG CATTCCTATC AAGAAATCCG ATCCCGTCGT GTCTTACCGG 
GAGACGGTCA GCGAGGAGTC CAACGTGATG TGCCTTTCCA AGTCCCCCAA CAAACACAAC 
AGGCTGTACA TGAAGGCCCG GCCCTTCCCG GATGGTTTGG CTGAAGACAT CGACAAGGGC 
GAGGTCTCTG CTCGCCAGGA GCTGAAGCAG AGGGCTCGCT ACCTGGCTGA GAAGTACGAG 
TGGGACGTCA CCGAGGCCAG GAAGATCTGG TGCTTCGGTC CTGACGGCAC CGGGCCCAAC 
ATCCTCACCG ACATCACCAA GGGAGTGCAG TACCTGAATG AGATCAAGGA CAGCGTGGTG 
GCCGGCTTCC AGTGGGCGAC CAAGGAGGGC GTCCTGTGTG AGGAGAACAT GCGCGGTGTC 
CGCTTCGATG TGCACGATGT GACCCTGCAT GCCGACGCCA TCCACCGTGG GGGTGGGCAG 
ATCATCCCCA CTGCCAGGAG GTGCCTGTAT GCCTGCGTGC TCACCGCCCA GCCCAGGCTC 
ATGGAGCCCA TCTACCTTGT GGAAATCCAG TGCCCAGAGC AGGTGGTTGG TGGCATCTAC 
GGCGTGCTGA ACAGGAAGCG TGGCCACGTC TTTGAGGAGT CCCAGGTGGC CGGCACCCCC 
ATGTTCGTGG TGAAGGCCTA CCTGCCCGTC AACGAGTCCT TCGGTTTCAC AGCCGACCTG 
AGGTCCAACA CGGGCGGCCA GGCTTTCCCC CAGTGTGTGT TCGACCACTG GCAGATCCTG 
CCCGGCGATC CCTTCGACAG CGCCAGCCGC CCCTCCCAGG TGGTGGCCGA GACCCGCAAA 
CGTAAGGGGC TGAAGGAGGG CATCCCGGCC CTGGACAACT TCCTGGACAA ATTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_990699.1
CDS	31..2607
Translation	MVNFTVDQIRAIMDKKANIRNMSVIAHVDHGKSTLTDSLVCKAGIIASARAGETRFTDTRKDEQERCITIKSTAISLFYELSENDLAFIKQSKDGSGFLINLIDSPGHVDFSSEVTAALRVTDGALVVVDCVSGVCVQTETVLRQAIAERIKPVLMMNKMDRALLELQLDPEELYQTFQRIVENVNVIISTYGEGESGPMGNIMIDPVLGTVGFGSGLHGWAFTLKQFAEMYVAKFAAKGDAQMNPTERAKKVEDMMKKLWGDRYFDPATGKFSKSATGPDGKKLPRTFCQLILDPIFKVFDAIMTFKKEEAAKLIEKLDIKLDSEDKDKEGKPLLKAVMRRWLPAGDALLQMITIHLPSPVTAQKYRCELLYEGPPDDEAAIGIKNCDPRGSLMMYISKMVPTSDKGRFYAFGRVFSGLVSTGLKVRIMGPNYTPGKKEDLYLKPIQRTILMMGRYVEPIEDVPCGNIVGLVGVDQFLVKTGTITTFEHAHNMRVMKFSVSPVVRVAVEAKNPADLPKLVEGLKRLAKSDPMVQCIIEESGEHIIAGAGELHLEICLKDLEEDHACIPIKKSDPVVSYRETVSEESNVMCLSKSPNKHNRLYMKARPFPDGLAEDIDKGEVSARQELKQRARYLAEKYEWDVTEARKIWCFGPDGTGPNILTDITKGVQYLNEIKDSVVAGFQWATKEGVLCEENMRGVRFDVHDVTLHADAIHRGGGQIIPTARRCLYACVLTAQPRLMEPIYLVEIQCPEQVVGGIYGVLNRKRGHVFEESQVAGTPMFVVKAYLPVNESFGFTADLRSNTGGQAFPQCVFDHWQILPGDPFDSASRPSQVVAETRKRKGLKEGIPALDNFLDKL

Target ORF information:

RefSeq Version	NM_205368.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus eukaryotic translation elongation factor 2 (EEF2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_205368.1

ORF Insert Sequence:

ATGGTGAACT TCACAGTAGA TCAGATACGG GCCATCATGG ACAAAAAGGC CAACATCAGA 
AACATGTCGG TGATCGCCCA CGTTGATCAC GGCAAGTCCA CCCTGACCGA TTCTCTGGTG 
TGCAAAGCTG GGATCATCGC CTCTGCGCGC GCGGGGGAGA CCCGTTTCAC TGACACGAGG 
AAGGACGAGC AGGAACGGTG CATCACCATC AAATCTACAG CTATTTCTCT GTTCTACGAG 
CTCTCGGAGA ACGATCTGGC GTTCATCAAG CAAAGCAAGG ATGGTTCTGG TTTCTTGATC 
AACCTGATCG ACTCTCCTGG GCACGTGGAT TTCTCTTCAG AGGTCACCGC TGCTCTGCGT 
GTCACTGATG GTGCCCTGGT TGTTGTCGAC TGCGTCTCTG GTGTGTGCGT GCAGACAGAA 
ACTGTGCTGC GTCAGGCCAT TGCTGAGAGG ATCAAACCTG TCCTGATGAT GAACAAGATG 
GATCGGGCGC TGCTGGAGCT GCAGCTGGAT CCTGAGGAGC TGTACCAGAC CTTCCAGCGG 
ATTGTGGAGA ACGTGAACGT CATCATCTCC ACGTATGGAG AAGGAGAAAG CGGCCCGATG 
GGAAATATCA TGATCGATCC TGTGCTTGGT ACAGTTGGCT TTGGTTCTGG CCTGCATGGC 
TGGGCTTTCA CTTTGAAACA GTTTGCTGAA ATGTATGTTG CAAAGTTTGC TGCCAAGGGT 
GATGCCCAGA TGAATCCAAC TGAACGTGCC AAGAAAGTAG AAGACATGAT GAAGAAGCTG 
TGGGGAGACA GATACTTTGA TCCTGCTACT GGCAAGTTCA GCAAATCCGC TACTGGCCCC 
GATGGAAAGA AACTGCCTAG GACCTTCTGC CAGCTCATCC TCGATCCCAT CTTCAAGGTG 
TTTGATGCGA TCATGACCTT CAAGAAAGAG GAGGCAGCTA AACTGATCGA GAAACTGGAC 
ATCAAGCTTG ACAGTGAAGA TAAGGACAAG GAGGGCAAAC CCCTGCTGAA GGCTGTGATG 
AGGCGGTGGC TGCCTGCCGG AGATGCCCTG CTCCAGATGA TCACCATTCA CCTGCCTTCT 
CCTGTCACAG CCCAGAAGTA CCGCTGTGAG CTGCTCTATG AGGGGCCCCC TGATGATGAG 
GCCGCCATAG GTATTAAGAA CTGTGATCCA AGGGGATCTC TGATGATGTA CATTTCTAAA 
ATGGTGCCAA CCTCCGACAA GGGACGTTTC TATGCTTTCG GCCGTGTTTT CTCTGGTCTC 
GTATCAACTG GCTTGAAAGT CAGAATCATG GGACCCAACT ACACGCCCGG CAAGAAGGAG 
GATCTGTACC TGAAGCCAAT CCAAAGGACC ATCCTCATGA TGGGCCGCTA CGTTGAACCC 
ATTGAGGACG TGCCTTGTGG AAACATTGTC GGTCTGGTTG GTGTTGATCA GTTCCTGGTG 
AAGACTGGAA CCATCACCAC CTTCGAGCAC GCTCACAACA TGCGGGTCAT GAAGTTCAGC 
GTCAGCCCTG TGGTGCGCGT GGCCGTGGAA GCCAAGAACC CTGCAGACCT GCCCAAGCTG 
GTGGAGGGGC TGAAGCGCCT TGCCAAGTCT GACCCTATGG TGCAGTGCAT CATTGAGGAG 
TCCGGAGAGC ACATCATTGC TGGTGCAGGG GAGCTGCACT TGGAAATCTG CCTGAAGGAT 
CTGGAGGAGG ACCACGCTTG CATTCCTATC AAGAAATCCG ATCCCGTCGT GTCTTACCGG 
GAGACGGTCA GCGAGGAGTC CAACGTGATG TGCCTTTCCA AGTCCCCCAA CAAACACAAC 
AGGCTGTACA TGAAGGCCCG GCCCTTCCCG GATGGTTTGG CTGAAGACAT CGACAAGGGC 
GAGGTCTCTG CTCGCCAGGA GCTGAAGCAG AGGGCTCGCT ACCTGGCTGA GAAGTACGAG 
TGGGACGTCA CCGAGGCCAG GAAGATCTGG TGCTTCGGTC CTGACGGCAC CGGGCCCAAC 
ATCCTCACCG ACATCACCAA GGGAGTGCAG TACCTGAATG AGATCAAGGA CAGCGTGGTG 
GCCGGCTTCC AGTGGGCGAC CAAGGAGGGC GTCCTGTGTG AGGAGAACAT GCGCGGTGTC 
CGCTTCGATG TGCACGATGT GACCCTGCAT GCCGACGCCA TCCACCGTGG GGGTGGGCAG 
ATCATCCCCA CTGCCAGGAG GTGCCTGTAT GCCTGCGTGC TCACCGCCCA GCCCAGGCTC 
ATGGAGCCCA TCTACCTTGT GGAAATCCAG TGCCCAGAGC AGGTGGTTGG TGGCATCTAC 
GGCGTGCTGA ACAGGAAGCG TGGCCACGTC TTTGAGGAGT CCCAGGTGGC CGGCACCCCC 
ATGTTCGTGG TGAAGGCCTA CCTGCCCGTC AACGAGTCCT TCGGTTTCAC AGCCGACCTG 
AGGTCCAACA CGGGCGGCCA GGCTTTCCCC CAGTGTGTGT TCGACCACTG GCAGATCCTG 
CCCGGCGATC CCTTCGACAG CGCCAGCCGC CCCTCCCAGG TGGTGGCCGA GACCCGCAAA 
CGTAAGGGGC TGAAGGAGGG CATCCCGGCC CTGGACAACT TCCTGGACAA ATTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Afferent regulation of chicken auditory brainstem neurons: rapid changes in phosphorylation of elongation factor 2.
The Journal of comparative neurology521(5)1165-83(2013 Apr)
McBride EG,Rubel EW,Wang Y

Regulation of neurite outgrowth mediated by localized phosphorylation of protein translational factor eEF2 in growth cones.
Developmental neurobiology73(3)230-46(2013 Mar)
Iketani M,Iizuka A,Sengoku K,Kurihara Y,Nakamura F,Sasaki Y,Sato Y,Yamane M,Matsushita M,Nairn AC,Takamatsu K,Goshima Y,Takei K

Glutamate-dependent elongation factor-2 phosphorylation in Bergmann glial cells.
Neurochemistry international52(6)1167-75(2008 May)
Barrera I,Hern?ndez-Kelly LC,Castel?n F,Ortega A

Functional characterization of the promoter region of the chicken elongation factor-2 gene.
Gene386(1-2)183-90(2007 Jan)
Lim EJ,Kim CW

Elongation factor-2 in chick embryo is phosphorylated on tyrosine as well as serine and threonine.
Biochemical and biophysical research communications175(2)400-6(1991 Mar)
Kim YW,Kim CW,Kang KR,Byun SM,Kang YS

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EEF2 cDNA ORF clone, Gallus gallus(chicken)

Gene

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gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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