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MELK cDNA ORF clone, Gallus gallus(chicken)

Summary

Gene Symbol MELK
Entrez Gene ID 428391
Full Name maternal embryonic leucine zipper kinase
General protein information
Preferred Names

maternal embryonic leucine zipper kinase

Names

maternal embryonic leucine zipper kinase
Gene Type protein-coding
Organism Gallus gallus(chicken)

Reference Sequences (RefSeq)

mRNA and Protein(s)

mRNA Protein Name
XM_025144751.1 XP_025000519.1 maternal embryonic leucine zipper kinase isoform X1
XM_025144752.1 XP_025000520.1 maternal embryonic leucine zipper kinase isoform X2
XM_015280794.2 XP_015136280.1 maternal embryonic leucine zipper kinase isoform X3
XM_015280794.1 XP_015136280.1 maternal embryonic leucine zipper kinase isoform X1
NM_001031509.1 NP_001026680.1 maternal embryonic leucine zipper kinase

Pathways from BioSystems


Homologies


Canis lupus familiaris (dog) MELK XP_538730.2
Gallus gallus (chicken) MELK NP_001026680.1
Xenopus tropicalis (tropical clawed frog) melk NP_001016390.1
Rattus norvegicus (Norway rat) Melk NP_001102132.1
Pan troglodytes (chimpanzee) MELK XP_001169038.1
Mus musculus (house mouse) Melk NP_034920.2
Danio rerio (zebrafish) melk NP_996771.2
Arabidopsis thaliana (thale cress) CIPK7 NP_188940.1
Homo sapiens (human) MELK NP_055606.1
MELK XP_001115076.2
Bos taurus (cattle) MELK NP_001104730.1
Caenorhabditis elegans (roundworm) pig-1 NP_001023420.2
Arabidopsis thaliana (thale cress) CIPK4 NP_193194.1

Gene Ontology


Bibliography

Related articles in PubMed

Full-length cDNAs from chicken bursal lymphocytes to facilitate gene function analysis.
Caldwell RB, Kierzek AM, Arakawa H, Bezzubov Y, Zaim J, Fiedler P, Kutter S, Blagodatski A, Kostovska D, Koter M, Plachy J, Carninci P, Hayashizaki Y, Buerstedde JM
Genome biology6(1)R6(2005)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following MELK gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the MELK cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OGa00491 NM_001031509.1
Latest version!		 Gallus gallus maternal embryonic leucine zipper kinase (MELK), mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $342.30
$489.00
OGa152997 XM_025144751.1
Latest version!		 Gallus gallus maternal embryonic leucine zipper kinase (MELK), transcript variant X1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 14-16 $307.30
$439.00
OGa152998 XM_025144752.1
Latest version!		 Gallus gallus maternal embryonic leucine zipper kinase (MELK), transcript variant X2, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 14-16 $307.30
$439.00
OGa48364 XM_015280794.2
Latest version!		 Gallus gallus maternal embryonic leucine zipper kinase (MELK), transcript variant X3, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 14-16 $342.30
$489.00
View Old Accession Versions >>
OGa48364 XM_015280794.1 Gallus gallus maternal embryonic leucine zipper kinase (MELK), transcript variant X1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 14-16 $342.30
$489.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)


CloneID OGa00491
Clone ID Related Accession (Same CDS sequence) NM_001031509.1
Accession Version NM_001031509.1 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 1974bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2018-05-20
Organism Gallus gallus(chicken)
Product maternal embryonic leucine zipper kinase
Comment Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AJ719849.1. On Aug 8, 2005 this sequence version replaced XM_425951.1. ##Evidence-Data-START## Transcript exon combination :: AJ719849.1 [ECO:0000332] ##Evidence-Data-END## 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
ATGTCTGTCA GTGACTACGA GGAAATTCTC AAGTACTATG AATTACATGA AACAATTGGA 
ACAGGTGGTT TTGCAAAGGT AAAACTCGGA CGGCATCTTC TTACTGGTGA AAAAGTTGCA 
ATAAAAATCA TGGACAAAGT TGCTTTAGGG GATGACTTGC CTCGAGTTAA AACAGAAATT 
GATGCCATGA AGAACTTAAG TCATCAACAT GTTTGCCGAT TGTACCATGT AATAGAGACA 
TCAAAGAAAA TATTCATGGT CTTAGGGTAT TGTCCCGGAG GAGAGCTGTT TGATTACATT 
GTTTCTAAGG ACCGCCTTTC GGAAGAGGAA GCTCGTGTCT TTTTTCGACA GATTGTTTCA 
GCAATTGCTT ATGTTCACAG TCAGGGTTAT GCCCACAGAG ACCTCAAACC AGAAAATCTG 
CTGATTGATG AGGAACATAA TTTGAAGCTG ATAGACTTCG GACTCTGTGC AAAGCCAAAG 
GGAGGCCTCG ATTACCGCCT GAACACATGC TGTGGAAGCC CAGCATACGC AGCACCAGAG 
CTAATCCAGG GCAAAGCATA TATTGGCTCA GAGGCAGATA TTTGGAGCAT GGGTGTACTG 
CTGTATGCTC TGCTGTGTGG GTTTCTTCCT TTTGATGATG ACAATGTGAT GGCACTCTAC 
AGGAGGATAA CAAGAGGAAA ATACGCTGTT CCAAAATGGC TTTCACCTAG CAGTACGCTG 
CTGCTTAACC AACTGCTACA GGTAGACCCA AAGAAGCGGA TTACAGTTAA ACACCTGTTA 
AGTCACCCTT GGCTCATGCA AGGTTATTCT GATGCTGTTC AGTGGCAAAG TAAATACCCA 
CTGGGACATC TTGATGAAGA CTGTGTAACA GAGCTTTCTG TGTTTCACAA CAAGAGCAGA 
GAGAATATAT CAGCATTAAT ATCAAAGTGG AATTATGACC AGATGTCAGC AACCTTTCTC 
TTGCTTCAAT CCAAGAAGTT TCGTGGCAAG CGCATTCACT TAAGAATTCC ATCACAAACA 
GGACATGCCA GTACAACACA GTCAGCAAGC CTGGGGTCTG AAAAGGCTAT GACATATGAA 
GATGTGCCAG ACTGCAGCGA TGTGCCAGAT GCGTTTGGAT CTATGGAATT CTCTGATGCA 
GCTTCACTGT TTGAGGAATC TCCACTAGAA GAGTTTAGTC TAAATACTCA CAGAACAAAG 
CAGCATTCGA GGCATGATAC TCAACTGGGT GATATGGAAT TCACACTGTC AACTCCAGTG 
ACAAGAAAAG TGGCATCTAA GAAGCATGCA AACAAAGAGA ATGTTGATGC AGAGTCAGCA 
TTAAGAAATG AGCTGCCCTT TGCACTTCCT GCTCCAAAGG CCTCCTTTTC AAAGAGTCAG 
ACAGAGAAGC AAGTACAAGG CATACCCTTT CAGGCACCTA CTTCCAAAGA GAGCCAGTTC 
ACGACAGTCA CCCCAGTTAA GAAGCCCAAA ACACCAACAA ATACATGTGA ACTGACAGCA 
ACTGAGGTTC TTCCTGAGAA AAGGTGTCAT TCTGTGGATT TAGATCTCAA CCGAGGTCAT 
GTGGATAGCA GCCAAAAGAA GAGAAAGGCC AAACTATTTG GAAGTCTCGA AAGAGGCCTA 
GATAAAGTGA TTACAATGCT TACACCAGGC AAAAAGAAGC GATTCAGTAG ACCTGGGCCA 
AGAAAACTTA AGGCGCACTA TAATGTTACC ATGACTCAAC TACTGAATCC AGACCAACTG 
CTGAATGAAA TAATCTCTGT CCTTTCAAAG AAGCAAGTGG AATACGTTAA GAAGGGCTAC 
ACCCTGAAAT GCCAAACAAA GCCAGATTTT GGCAGAGAAT CTATGAAGTT TGAGTTAGAA 
GTGTGTCGGC TCAGTAAGAA TGGAGCGGTT GGTATCAGGA GGCAGAGGCT TAGAGGGGAT 
GCTTGGGTCT ACAAGAGGCT GGTGGAAGAC ATTTTATCCA GCTGCCAAGT GTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001026680.1
CDS163..2136
Translation

Target ORF information:

RefSeq Version NM_001031509.1
Organism Gallus gallus(chicken)
Definition Gallus gallus maternal embryonic leucine zipper kinase (MELK), mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 NM_001031509.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
ATGTCTGTCA GTGACTACGA GGAAATTCTC AAGTACTATG AATTACATGA AACAATTGGA 
ACAGGTGGTT TTGCAAAGGT AAAACTCGGA CGGCATCTTC TTACTGGTGA AAAAGTTGCA 
ATAAAAATCA TGGACAAAGT TGCTTTAGGG GATGACTTGC CTCGAGTTAA AACAGAAATT 
GATGCCATGA AGAACTTAAG TCATCAACAT GTTTGCCGAT TGTACCATGT AATAGAGACA 
TCAAAGAAAA TATTCATGGT CTTAGGGTAT TGTCCCGGAG GAGAGCTGTT TGATTACATT 
GTTTCTAAGG ACCGCCTTTC GGAAGAGGAA GCTCGTGTCT TTTTTCGACA GATTGTTTCA 
GCAATTGCTT ATGTTCACAG TCAGGGTTAT GCCCACAGAG ACCTCAAACC AGAAAATCTG 
CTGATTGATG AGGAACATAA TTTGAAGCTG ATAGACTTCG GACTCTGTGC AAAGCCAAAG 
GGAGGCCTCG ATTACCGCCT GAACACATGC TGTGGAAGCC CAGCATACGC AGCACCAGAG 
CTAATCCAGG GCAAAGCATA TATTGGCTCA GAGGCAGATA TTTGGAGCAT GGGTGTACTG 
CTGTATGCTC TGCTGTGTGG GTTTCTTCCT TTTGATGATG ACAATGTGAT GGCACTCTAC 
AGGAGGATAA CAAGAGGAAA ATACGCTGTT CCAAAATGGC TTTCACCTAG CAGTACGCTG 
CTGCTTAACC AACTGCTACA GGTAGACCCA AAGAAGCGGA TTACAGTTAA ACACCTGTTA 
AGTCACCCTT GGCTCATGCA AGGTTATTCT GATGCTGTTC AGTGGCAAAG TAAATACCCA 
CTGGGACATC TTGATGAAGA CTGTGTAACA GAGCTTTCTG TGTTTCACAA CAAGAGCAGA 
GAGAATATAT CAGCATTAAT ATCAAAGTGG AATTATGACC AGATGTCAGC AACCTTTCTC 
TTGCTTCAAT CCAAGAAGTT TCGTGGCAAG CGCATTCACT TAAGAATTCC ATCACAAACA 
GGACATGCCA GTACAACACA GTCAGCAAGC CTGGGGTCTG AAAAGGCTAT GACATATGAA 
GATGTGCCAG ACTGCAGCGA TGTGCCAGAT GCGTTTGGAT CTATGGAATT CTCTGATGCA 
GCTTCACTGT TTGAGGAATC TCCACTAGAA GAGTTTAGTC TAAATACTCA CAGAACAAAG 
CAGCATTCGA GGCATGATAC TCAACTGGGT GATATGGAAT TCACACTGTC AACTCCAGTG 
ACAAGAAAAG TGGCATCTAA GAAGCATGCA AACAAAGAGA ATGTTGATGC AGAGTCAGCA 
TTAAGAAATG AGCTGCCCTT TGCACTTCCT GCTCCAAAGG CCTCCTTTTC AAAGAGTCAG 
ACAGAGAAGC AAGTACAAGG CATACCCTTT CAGGCACCTA CTTCCAAAGA GAGCCAGTTC 
ACGACAGTCA CCCCAGTTAA GAAGCCCAAA ACACCAACAA ATACATGTGA ACTGACAGCA 
ACTGAGGTTC TTCCTGAGAA AAGGTGTCAT TCTGTGGATT TAGATCTCAA CCGAGGTCAT 
GTGGATAGCA GCCAAAAGAA GAGAAAGGCC AAACTATTTG GAAGTCTCGA AAGAGGCCTA 
GATAAAGTGA TTACAATGCT TACACCAGGC AAAAAGAAGC GATTCAGTAG ACCTGGGCCA 
AGAAAACTTA AGGCGCACTA TAATGTTACC ATGACTCAAC TACTGAATCC AGACCAACTG 
CTGAATGAAA TAATCTCTGT CCTTTCAAAG AAGCAAGTGG AATACGTTAA GAAGGGCTAC 
ACCCTGAAAT GCCAAACAAA GCCAGATTTT GGCAGAGAAT CTATGAAGTT TGAGTTAGAA 
GTGTGTCGGC TCAGTAAGAA TGGAGCGGTT GGTATCAGGA GGCAGAGGCT TAGAGGGGAT 
GCTTGGGTCT ACAAGAGGCT GGTGGAAGAC ATTTTATCCA GCTGCCAAGT GTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OGa152997
Clone ID Related Accession (Same CDS sequence) XM_025144751.1
Accession Version XM_025144751.1 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 1875bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2018-05-16
Organism Gallus gallus(chicken)
Product maternal embryonic leucine zipper kinase isoform X1
Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006127.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
ATGTCTGTCA GTGACTACGA GGAAATTCTC AAGTACTATG AATTACATGA AACAATTGGA 
ACGGGTGGTT TTGCAAAGGT AAAACTCGGA CGGCATCTTC TTACTGGTGA AAAAGTTGCA 
ATAAAAATCA TGGACAAAGT TGCTTTAGGG GATGACTTGC CTCGAGTTAA AACAGAAATT 
GATGCCATGA AGAACTTAAG TCATCAACAT GTTTGCCGAT TGTACCATGT AATAGAGACA 
TCAAAGAAAA TATTCATGGT CTTAGAGTAT TGTCCCGGAG GAGAGCTGTT TGATTACATT 
GTTTCTAAGG ACCGCCTTTC GGAAGAGGAA GCTCGTGTCT TTTTTCGACA GATTGTTTCA 
GCAATTGCTT ATGTTCACAG TCAGGGTTAT GCCCACAGAG ACCTCAAACC AGAAAATCTG 
CTGATTGATG AGGAACATAA TTTGAAGCTG ATAGACTTCG GACTCTGTGC AAAGCCAAAG 
GGAGGCCTCG ATTACCGCCT GAACACATGC TGTGGAAGCC CAGCATACGC AGCACCAGAG 
CTAATCCAGG GCAAAGCGTA TATTGGCTCA GAGGCAGATA TTTGGAGCAT GGGTGTACTG 
CTGTATGCTC TGCTGTGTGG GTTTCTTCCT TTTGATGATG ACAATGTGAT GGCACTCTAC 
AGGAGGATAA CAAGAGGAAA ATACGCTGTT CCAAAATGGC TTTCACCTAG CAGTACGCTG 
CTGCTTAACC AACTGCTACA GCTGGGACAT CTTGATGAAG ACTGTGTAAC AGAGCTTTCT 
GTGTTTCACA ACAAGAGCAG AGAGAATATA TCAGCATTAA TATCAAAGTG GAATTACGAC 
CAGATGTCAG CAACCTATCT CTTGCTTCAA TCCAAGAAGT TTCGTGGCAA GCGCATTCAC 
TTAAGAATTC CATCACAAAC AGGACATGCC AGTACAACAC AGTCAGCAAG CCTGGGGTCT 
GAAAAGGCTA TGACATATGA AGATGTGCCA GACTGCAGCG ATGTGCCAGA TGCGTTTGGA 
TCTATGGAAT TCTCTGATGC AGCTTCACTG TTTGAGGAAT CTCCACTAGA AGAGTTTAGT 
CTAAATACTC ACAGAACAAA GCAGCATTCG AGGCATGATA CTCAACTGGG TGATATGGAA 
TTCACACTGT CAACTCCAGT GACAAGAAAA GTGGCATCGA AGAAGCATGC AAACAAAGAG 
AATGTTGATG CAGAGTCAGC ATTAAGAAAT GAGCTGCCCT TTGCACTTCC TGCTCCAAAG 
GCCTCCTTTT CAAAGAGTCA GACAGAGAAG CAAGTACAAG GCATACCCTT TCAGGCACCT 
ACTTCCAAAG AGAGCCAGTT CACGACAGTC ACCCCAGTTA AGAAGCCCAA AACACCAACA 
AATACATGTG AACTGACAGC AACTGAGGTT CTTACTGAGA AAAGGTGTCA TTCTGTGGAT 
TTAGATCTCA ACCGAGGTCA TGTGGATAGC AGCCAAAAGA AGAGAAAGGC CAAACTATTT 
GGAAGTCTCG AAAGAGGCCT AGATAAAGTG ATTACAATGC TTACACCAGG CAAAAAGAAG 
CGATTCAGTA GACCTGGGCC AAGAAAACTT AAGGCGCACT ATAATGTTAC CATGACTCAA 
CTACTGAATC CAGACCAACT GCTGAATGAA ATAATCTCTA TCCTTTCAAA GAAGCAAGTG 
GAATACGTTA AGAAGGGCTA CACCCTGAAA TGCCAAACAA AGCCAGATTT TGGCAGAGAA 
TCTATGAAGT TTGAGTTAGA AGTGTGTCGG CTCAGTAAGA ATGGAGCGGT TGGTATCAGG 
AGGCAGAGGC TTAGAGGGGA TGCTTGGGTC TACAAGAGGC TGGTGGAAGA CATTTTATCC 
AGCTGCCAAG TGTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_025000519.1
CDS155..2029
Translation

Target ORF information:

RefSeq Version XM_025144751.1
Organism Gallus gallus(chicken)
Definition Gallus gallus maternal embryonic leucine zipper kinase (MELK), transcript variant X1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 XM_025144751.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
ATGTCTGTCA GTGACTACGA GGAAATTCTC AAGTACTATG AATTACATGA AACAATTGGA 
ACGGGTGGTT TTGCAAAGGT AAAACTCGGA CGGCATCTTC TTACTGGTGA AAAAGTTGCA 
ATAAAAATCA TGGACAAAGT TGCTTTAGGG GATGACTTGC CTCGAGTTAA AACAGAAATT 
GATGCCATGA AGAACTTAAG TCATCAACAT GTTTGCCGAT TGTACCATGT AATAGAGACA 
TCAAAGAAAA TATTCATGGT CTTAGAGTAT TGTCCCGGAG GAGAGCTGTT TGATTACATT 
GTTTCTAAGG ACCGCCTTTC GGAAGAGGAA GCTCGTGTCT TTTTTCGACA GATTGTTTCA 
GCAATTGCTT ATGTTCACAG TCAGGGTTAT GCCCACAGAG ACCTCAAACC AGAAAATCTG 
CTGATTGATG AGGAACATAA TTTGAAGCTG ATAGACTTCG GACTCTGTGC AAAGCCAAAG 
GGAGGCCTCG ATTACCGCCT GAACACATGC TGTGGAAGCC CAGCATACGC AGCACCAGAG 
CTAATCCAGG GCAAAGCGTA TATTGGCTCA GAGGCAGATA TTTGGAGCAT GGGTGTACTG 
CTGTATGCTC TGCTGTGTGG GTTTCTTCCT TTTGATGATG ACAATGTGAT GGCACTCTAC 
AGGAGGATAA CAAGAGGAAA ATACGCTGTT CCAAAATGGC TTTCACCTAG CAGTACGCTG 
CTGCTTAACC AACTGCTACA GCTGGGACAT CTTGATGAAG ACTGTGTAAC AGAGCTTTCT 
GTGTTTCACA ACAAGAGCAG AGAGAATATA TCAGCATTAA TATCAAAGTG GAATTACGAC 
CAGATGTCAG CAACCTATCT CTTGCTTCAA TCCAAGAAGT TTCGTGGCAA GCGCATTCAC 
TTAAGAATTC CATCACAAAC AGGACATGCC AGTACAACAC AGTCAGCAAG CCTGGGGTCT 
GAAAAGGCTA TGACATATGA AGATGTGCCA GACTGCAGCG ATGTGCCAGA TGCGTTTGGA 
TCTATGGAAT TCTCTGATGC AGCTTCACTG TTTGAGGAAT CTCCACTAGA AGAGTTTAGT 
CTAAATACTC ACAGAACAAA GCAGCATTCG AGGCATGATA CTCAACTGGG TGATATGGAA 
TTCACACTGT CAACTCCAGT GACAAGAAAA GTGGCATCGA AGAAGCATGC AAACAAAGAG 
AATGTTGATG CAGAGTCAGC ATTAAGAAAT GAGCTGCCCT TTGCACTTCC TGCTCCAAAG 
GCCTCCTTTT CAAAGAGTCA GACAGAGAAG CAAGTACAAG GCATACCCTT TCAGGCACCT 
ACTTCCAAAG AGAGCCAGTT CACGACAGTC ACCCCAGTTA AGAAGCCCAA AACACCAACA 
AATACATGTG AACTGACAGC AACTGAGGTT CTTACTGAGA AAAGGTGTCA TTCTGTGGAT 
TTAGATCTCA ACCGAGGTCA TGTGGATAGC AGCCAAAAGA AGAGAAAGGC CAAACTATTT 
GGAAGTCTCG AAAGAGGCCT AGATAAAGTG ATTACAATGC TTACACCAGG CAAAAAGAAG 
CGATTCAGTA GACCTGGGCC AAGAAAACTT AAGGCGCACT ATAATGTTAC CATGACTCAA 
CTACTGAATC CAGACCAACT GCTGAATGAA ATAATCTCTA TCCTTTCAAA GAAGCAAGTG 
GAATACGTTA AGAAGGGCTA CACCCTGAAA TGCCAAACAA AGCCAGATTT TGGCAGAGAA 
TCTATGAAGT TTGAGTTAGA AGTGTGTCGG CTCAGTAAGA ATGGAGCGGT TGGTATCAGG 
AGGCAGAGGC TTAGAGGGGA TGCTTGGGTC TACAAGAGGC TGGTGGAAGA CATTTTATCC 
AGCTGCCAAG TGTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OGa152998
Clone ID Related Accession (Same CDS sequence) XM_025144752.1
Accession Version XM_025144752.1 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 1827bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2018-05-16
Organism Gallus gallus(chicken)
Product maternal embryonic leucine zipper kinase isoform X2
Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006127.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
ATGTCTGTCA GTGACTACGA GGAAATTCTC AAGTACTATG AATTACATGA AACAATTGGA 
ACGGGTGGTT TTGCAAAGGT AAAACTCGGA CGGCATCTTC TTACTGGTGA AAAAGTTGCA 
ATAAAAATCA TGGACAAAGT TGCTTTAGGG GATGACTTGC CTCGAGTTAA AACAGAAATT 
GATGCCATGA AGAACTTAAG TCATCAACAT GTTTGCCGAT TGTACCATGT AATAGAGACA 
TCAAAGAAAA TATTCATGGT CTTAGAGTAT TGTCCCGGAG GAGAGCTGTT TGATTACATT 
GTTTCTAAGG ACCGCCTTTC GGAAGAGGAA GCTCGTGTCT TTTTTCGACA GATTGTTTCA 
GCAATTGCTT ATGTTCACAG TCAGGGTTAT GCCCACAGAG ACCTCAAACC AGAAAATCTG 
CTGATTGATG AGGAACATAA TTTGAAGCTG ATAGACTTCG GACTCTGTGC AAAGCCAAAG 
GGAGGCCTCG ATTACCGCCT GAACACATGC TGTGGAAGCC CAGCATACGC AGCACCAGAG 
CTAATCCAGG GCAAAGCGTA TATTGGCTCA GAGGCAGATA TTTGGAGCAT GGGTGTACTG 
CTGTATGCTC TGCTGTGTGG GTTTCTTCCT TTTGATGATG ACAATGTGAT GGCACTCTAC 
AGGAGGATAA CAAGAGGAAA ATACGCTGTT CCAAAATGGC TTTCACCTAG CAGTACGCTG 
CTGCTTAACC AACTGCTACA GGTAGACCCA AAGAAGCGGA TTACAGTTAA ACACCTGTTA 
AGTCACCCTT GGCTCATGCA AGGTTATTCT GATGCTGTTC AGTGGCAAAG TAAATACCCA 
CTGGGACATC TTGATGAAGA CTGTGTAACA GAGCTTTCTG TGTTTCACAA CAAGAGCAGA 
GAGAATATAT CAGCATTAAT ATCAAAGTGG AATTACGACC AGATGTCAGC AACCTATCTC 
TTGCTTCAAT CCAAGAAGTT TCGTGGCAAG CGCATTCACT TAAGAATTCC ATCACAAACA 
GGACATGCCA GTACAACACA GTCAGCAAGC CTGGGGCATT CGAGGCATGA TACTCAACTG 
GGTGATATGG AATTCACACT GTCAACTCCA GTGACAAGAA AAGTGGCATC GAAGAAGCAT 
GCAAACAAAG AGAATGTTGA TGCAGAGTCA GCATTAAGAA ATGAGCTGCC CTTTGCACTT 
CCTGCTCCAA AGGCCTCCTT TTCAAAGAGT CAGACAGAGA AGCAAGTACA AGGCATACCC 
TTTCAGGCAC CTACTTCCAA AGAGAGCCAG TTCACGACAG TCACCCCAGT TAAGAAGCCC 
AAAACACCAA CAAATACATG TGAACTGACA GCAACTGAGG TTCTTACTGA GAAAAGGTGT 
CATTCTGTGG ATTTAGATCT CAACCGAGGT CATGTGGATA GCAGCCAAAA GAAGAGAAAG 
GCCAAACTAT TTGGAAGTCT CGAAAGAGGC CTAGATAAAG TGATTACAAT GCTTACACCA 
GGCAAAAAGA AGCGATTCAG TAGACCTGGG CCAAGAAAAC TTAAGGCGCA CTATAATGTT 
ACCATGACTC AACTACTGAA TCCAGACCAA CTGCTGAATG AAATAATCTC TATCCTTTCA 
AAGAAGCAAG TGGAATACGT TAAGAAGGGC TACACCCTGA AATGCCAAAC AAAGCCAGAT 
TTTGGCAGAG AATCTATGAA GTTTGAGTTA GAAGTGTGTC GGCTCAGTAA GAATGGAGCG 
GTTGGTATCA GGAGGCAGAG GCTTAGAGGG GATGCTTGGG TCTACAAGAG GCTGGTGGAA 
GACATTTTAT CCAGCTGCCA AGTGTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_025000520.1
CDS155..1981
Translation

Target ORF information:

RefSeq Version XM_025144752.1
Organism Gallus gallus(chicken)
Definition Gallus gallus maternal embryonic leucine zipper kinase (MELK), transcript variant X2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 XM_025144752.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
ATGTCTGTCA GTGACTACGA GGAAATTCTC AAGTACTATG AATTACATGA AACAATTGGA 
ACGGGTGGTT TTGCAAAGGT AAAACTCGGA CGGCATCTTC TTACTGGTGA AAAAGTTGCA 
ATAAAAATCA TGGACAAAGT TGCTTTAGGG GATGACTTGC CTCGAGTTAA AACAGAAATT 
GATGCCATGA AGAACTTAAG TCATCAACAT GTTTGCCGAT TGTACCATGT AATAGAGACA 
TCAAAGAAAA TATTCATGGT CTTAGAGTAT TGTCCCGGAG GAGAGCTGTT TGATTACATT 
GTTTCTAAGG ACCGCCTTTC GGAAGAGGAA GCTCGTGTCT TTTTTCGACA GATTGTTTCA 
GCAATTGCTT ATGTTCACAG TCAGGGTTAT GCCCACAGAG ACCTCAAACC AGAAAATCTG 
CTGATTGATG AGGAACATAA TTTGAAGCTG ATAGACTTCG GACTCTGTGC AAAGCCAAAG 
GGAGGCCTCG ATTACCGCCT GAACACATGC TGTGGAAGCC CAGCATACGC AGCACCAGAG 
CTAATCCAGG GCAAAGCGTA TATTGGCTCA GAGGCAGATA TTTGGAGCAT GGGTGTACTG 
CTGTATGCTC TGCTGTGTGG GTTTCTTCCT TTTGATGATG ACAATGTGAT GGCACTCTAC 
AGGAGGATAA CAAGAGGAAA ATACGCTGTT CCAAAATGGC TTTCACCTAG CAGTACGCTG 
CTGCTTAACC AACTGCTACA GGTAGACCCA AAGAAGCGGA TTACAGTTAA ACACCTGTTA 
AGTCACCCTT GGCTCATGCA AGGTTATTCT GATGCTGTTC AGTGGCAAAG TAAATACCCA 
CTGGGACATC TTGATGAAGA CTGTGTAACA GAGCTTTCTG TGTTTCACAA CAAGAGCAGA 
GAGAATATAT CAGCATTAAT ATCAAAGTGG AATTACGACC AGATGTCAGC AACCTATCTC 
TTGCTTCAAT CCAAGAAGTT TCGTGGCAAG CGCATTCACT TAAGAATTCC ATCACAAACA 
GGACATGCCA GTACAACACA GTCAGCAAGC CTGGGGCATT CGAGGCATGA TACTCAACTG 
GGTGATATGG AATTCACACT GTCAACTCCA GTGACAAGAA AAGTGGCATC GAAGAAGCAT 
GCAAACAAAG AGAATGTTGA TGCAGAGTCA GCATTAAGAA ATGAGCTGCC CTTTGCACTT 
CCTGCTCCAA AGGCCTCCTT TTCAAAGAGT CAGACAGAGA AGCAAGTACA AGGCATACCC 
TTTCAGGCAC CTACTTCCAA AGAGAGCCAG TTCACGACAG TCACCCCAGT TAAGAAGCCC 
AAAACACCAA CAAATACATG TGAACTGACA GCAACTGAGG TTCTTACTGA GAAAAGGTGT 
CATTCTGTGG ATTTAGATCT CAACCGAGGT CATGTGGATA GCAGCCAAAA GAAGAGAAAG 
GCCAAACTAT TTGGAAGTCT CGAAAGAGGC CTAGATAAAG TGATTACAAT GCTTACACCA 
GGCAAAAAGA AGCGATTCAG TAGACCTGGG CCAAGAAAAC TTAAGGCGCA CTATAATGTT 
ACCATGACTC AACTACTGAA TCCAGACCAA CTGCTGAATG AAATAATCTC TATCCTTTCA 
AAGAAGCAAG TGGAATACGT TAAGAAGGGC TACACCCTGA AATGCCAAAC AAAGCCAGAT 
TTTGGCAGAG AATCTATGAA GTTTGAGTTA GAAGTGTGTC GGCTCAGTAA GAATGGAGCG 
GTTGGTATCA GGAGGCAGAG GCTTAGAGGG GATGCTTGGG TCTACAAGAG GCTGGTGGAA 
GACATTTTAT CCAGCTGCCA AGTGTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OGa48364
Clone ID Related Accession (Same CDS sequence) XM_015280794.2 , XM_015280794.1
Accession Version XM_015280794.2 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 1788bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2018-05-16
Organism Gallus gallus(chicken)
Product maternal embryonic leucine zipper kinase isoform X3
Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006127.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015280794.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
ATGTCTGTCA GTGACTACGA GGAAATTCTC AAGTACTATG AATTACATGA AACAATTGGA 
ACGGGTGGTT TTGCAAAGGT AAAACTCGGA CGGCATCTTC TTACTGGTGA AAAAGTTGCA 
ATAAAAATCA TGGACAAAGT TGCTTTAGGG GATGACTTGC CTCGAGTTAA AACAGAAATT 
GATGCCATGA AGAACTTAAG TCATCAACAT GTTTGCCGAT TGTACCATGT AATAGAGACA 
TCAAAGAAAA TATTCATGGT CTTAGAGTAT TGTCCCGGAG GAGAGCTGTT TGATTACATT 
GTTTCTAAGG ACCGCCTTTC GGAAGAGGAA GCTCGTGTCT TTTTTCGACA GATTGTTTCA 
GCAATTGCTT ATGTTCACAG TCAGGGTTAT GCCCACAGAG ACCTCAAACC AGAAAATCTG 
CTGATTGATG AGGAACATAA TTTGAAGCTG ATAGACTTCG GACTCTGTGC AAAGCCAAAG 
GGAGGCCTCG ATTACCGCCT GAACACATGC TGTGGAAGCC CAGCATACGC AGCACCAGAG 
CTAATCCAGG GCAAAGCGTA TATTGGCTCA GAGGCAGATA TTTGGAGCAT GGGTGTACTG 
CTGTATGCTC TGCTGTGTGG GTTTCTTCCT TTTGATGATG ACAATGTGAT GGCACTCTAC 
AGGAGGATAA CAAGAGGAAA ATACGCTGTT CCAAAATGGC TTTCACCTAG CAGTACGCTG 
CTGCTTAACC AACTGCTACA GTGGAATTAC GACCAGATGT CAGCAACCTA TCTCTTGCTT 
CAATCCAAGA AGTTTCGTGG CAAGCGCATT CACTTAAGAA TTCCATCACA AACAGGACAT 
GCCAGTACAA CACAGTCAGC AAGCCTGGGG TCTGAAAAGG CTATGACATA TGAAGATGTG 
CCAGACTGCA GCGATGTGCC AGATGCGTTT GGATCTATGG AATTCTCTGA TGCAGCTTCA 
CTGTTTGAGG AATCTCCACT AGAAGAGTTT AGTCTAAATA CTCACAGAAC AAAGCAGCAT 
TCGAGGCATG ATACTCAACT GGGTGATATG GAATTCACAC TGTCAACTCC AGTGACAAGA 
AAAGTGGCAT CGAAGAAGCA TGCAAACAAA GAGAATGTTG ATGCAGAGTC AGCATTAAGA 
AATGAGCTGC CCTTTGCACT TCCTGCTCCA AAGGCCTCCT TTTCAAAGAG TCAGACAGAG 
AAGCAAGTAC AAGGCATACC CTTTCAGGCA CCTACTTCCA AAGAGAGCCA GTTCACGACA 
GTCACCCCAG TTAAGAAGCC CAAAACACCA ACAAATACAT GTGAACTGAC AGCAACTGAG 
GTTCTTACTG AGAAAAGGTG TCATTCTGTG GATTTAGATC TCAACCGAGG TCATGTGGAT 
AGCAGCCAAA AGAAGAGAAA GGCCAAACTA TTTGGAAGTC TCGAAAGAGG CCTAGATAAA 
GTGATTACAA TGCTTACACC AGGCAAAAAG AAGCGATTCA GTAGACCTGG GCCAAGAAAA 
CTTAAGGCGC ACTATAATGT TACCATGACT CAACTACTGA ATCCAGACCA ACTGCTGAAT 
GAAATAATCT CTATCCTTTC AAAGAAGCAA GTGGAATACG TTAAGAAGGG CTACACCCTG 
AAATGCCAAA CAAAGCCAGA TTTTGGCAGA GAATCTATGA AGTTTGAGTT AGAAGTGTGT 
CGGCTCAGTA AGAATGGAGC GGTTGGTATC AGGAGGCAGA GGCTTAGAGG GGATGCTTGG 
GTCTACAAGA GGCTGGTGGA AGACATTTTA TCCAGCTGCC AAGTGTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_015136280.1
CDS155..1942
Translation

Target ORF information:

RefSeq Version XM_015280794.2
Organism Gallus gallus(chicken)
Definition Gallus gallus maternal embryonic leucine zipper kinase (MELK), transcript variant X3, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 XM_015280794.2

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
ATGTCTGTCA GTGACTACGA GGAAATTCTC AAGTACTATG AATTACATGA AACAATTGGA 
ACGGGTGGTT TTGCAAAGGT AAAACTCGGA CGGCATCTTC TTACTGGTGA AAAAGTTGCA 
ATAAAAATCA TGGACAAAGT TGCTTTAGGG GATGACTTGC CTCGAGTTAA AACAGAAATT 
GATGCCATGA AGAACTTAAG TCATCAACAT GTTTGCCGAT TGTACCATGT AATAGAGACA 
TCAAAGAAAA TATTCATGGT CTTAGAGTAT TGTCCCGGAG GAGAGCTGTT TGATTACATT 
GTTTCTAAGG ACCGCCTTTC GGAAGAGGAA GCTCGTGTCT TTTTTCGACA GATTGTTTCA 
GCAATTGCTT ATGTTCACAG TCAGGGTTAT GCCCACAGAG ACCTCAAACC AGAAAATCTG 
CTGATTGATG AGGAACATAA TTTGAAGCTG ATAGACTTCG GACTCTGTGC AAAGCCAAAG 
GGAGGCCTCG ATTACCGCCT GAACACATGC TGTGGAAGCC CAGCATACGC AGCACCAGAG 
CTAATCCAGG GCAAAGCGTA TATTGGCTCA GAGGCAGATA TTTGGAGCAT GGGTGTACTG 
CTGTATGCTC TGCTGTGTGG GTTTCTTCCT TTTGATGATG ACAATGTGAT GGCACTCTAC 
AGGAGGATAA CAAGAGGAAA ATACGCTGTT CCAAAATGGC TTTCACCTAG CAGTACGCTG 
CTGCTTAACC AACTGCTACA GTGGAATTAC GACCAGATGT CAGCAACCTA TCTCTTGCTT 
CAATCCAAGA AGTTTCGTGG CAAGCGCATT CACTTAAGAA TTCCATCACA AACAGGACAT 
GCCAGTACAA CACAGTCAGC AAGCCTGGGG TCTGAAAAGG CTATGACATA TGAAGATGTG 
CCAGACTGCA GCGATGTGCC AGATGCGTTT GGATCTATGG AATTCTCTGA TGCAGCTTCA 
CTGTTTGAGG AATCTCCACT AGAAGAGTTT AGTCTAAATA CTCACAGAAC AAAGCAGCAT 
TCGAGGCATG ATACTCAACT GGGTGATATG GAATTCACAC TGTCAACTCC AGTGACAAGA 
AAAGTGGCAT CGAAGAAGCA TGCAAACAAA GAGAATGTTG ATGCAGAGTC AGCATTAAGA 
AATGAGCTGC CCTTTGCACT TCCTGCTCCA AAGGCCTCCT TTTCAAAGAG TCAGACAGAG 
AAGCAAGTAC AAGGCATACC CTTTCAGGCA CCTACTTCCA AAGAGAGCCA GTTCACGACA 
GTCACCCCAG TTAAGAAGCC CAAAACACCA ACAAATACAT GTGAACTGAC AGCAACTGAG 
GTTCTTACTG AGAAAAGGTG TCATTCTGTG GATTTAGATC TCAACCGAGG TCATGTGGAT 
AGCAGCCAAA AGAAGAGAAA GGCCAAACTA TTTGGAAGTC TCGAAAGAGG CCTAGATAAA 
GTGATTACAA TGCTTACACC AGGCAAAAAG AAGCGATTCA GTAGACCTGG GCCAAGAAAA 
CTTAAGGCGC ACTATAATGT TACCATGACT CAACTACTGA ATCCAGACCA ACTGCTGAAT 
GAAATAATCT CTATCCTTTC AAAGAAGCAA GTGGAATACG TTAAGAAGGG CTACACCCTG 
AAATGCCAAA CAAAGCCAGA TTTTGGCAGA GAATCTATGA AGTTTGAGTT AGAAGTGTGT 
CGGCTCAGTA AGAATGGAGC GGTTGGTATC AGGAGGCAGA GGCTTAGAGG GGATGCTTGG 
GTCTACAAGA GGCTGGTGGA AGACATTTTA TCCAGCTGCC AAGTGTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OGa48364
Clone ID Related Accession (Same CDS sequence) XM_015280794.2 , XM_015280794.1
Accession Version XM_015280794.1 Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 1788bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2016-01-03
Organism Gallus gallus(chicken)
Product maternal embryonic leucine zipper kinase isoform X1
Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003764324.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
ATGTCTGTCA GTGACTACGA GGAAATTCTC AAGTACTATG AATTACATGA AACAATTGGA 
ACGGGTGGTT TTGCAAAGGT AAAACTCGGA CGGCATCTTC TTACTGGTGA AAAAGTTGCA 
ATAAAAATCA TGGACAAAGT TGCTTTAGGG GATGACTTGC CTCGAGTTAA AACAGAAATT 
GATGCCATGA AGAACTTAAG TCATCAACAT GTTTGCCGAT TGTACCATGT AATAGAGACA 
TCAAAGAAAA TATTCATGGT CTTAGAGTAT TGTCCCGGAG GAGAGCTGTT TGATTACATT 
GTTTCTAAGG ACCGCCTTTC GGAAGAGGAA GCTCGTGTCT TTTTTCGACA GATTGTTTCA 
GCAATTGCTT ATGTTCACAG TCAGGGTTAT GCCCACAGAG ACCTCAAACC AGAAAATCTG 
CTGATTGATG AGGAACATAA TTTGAAGCTG ATAGACTTCG GACTCTGTGC AAAGCCAAAG 
GGAGGCCTCG ATTACCGCCT GAACACATGC TGTGGAAGCC CAGCATACGC AGCACCAGAG 
CTAATCCAGG GCAAAGCGTA TATTGGCTCA GAGGCAGATA TTTGGAGCAT GGGTGTACTG 
CTGTATGCTC TGCTGTGTGG GTTTCTTCCT TTTGATGATG ACAATGTGAT GGCACTCTAC 
AGGAGGATAA CAAGAGGAAA ATACGCTGTT CCAAAATGGC TTTCACCTAG CAGTACGCTG 
CTGCTTAACC AACTGCTACA GTGGAATTAC GACCAGATGT CAGCAACCTA TCTCTTGCTT 
CAATCCAAGA AGTTTCGTGG CAAGCGCATT CACTTAAGAA TTCCATCACA AACAGGACAT 
GCCAGTACAA CACAGTCAGC AAGCCTGGGG TCTGAAAAGG CTATGACATA TGAAGATGTG 
CCAGACTGCA GCGATGTGCC AGATGCGTTT GGATCTATGG AATTCTCTGA TGCAGCTTCA 
CTGTTTGAGG AATCTCCACT AGAAGAGTTT AGTCTAAATA CTCACAGAAC AAAGCAGCAT 
TCGAGGCATG ATACTCAACT GGGTGATATG GAATTCACAC TGTCAACTCC AGTGACAAGA 
AAAGTGGCAT CGAAGAAGCA TGCAAACAAA GAGAATGTTG ATGCAGAGTC AGCATTAAGA 
AATGAGCTGC CCTTTGCACT TCCTGCTCCA AAGGCCTCCT TTTCAAAGAG TCAGACAGAG 
AAGCAAGTAC AAGGCATACC CTTTCAGGCA CCTACTTCCA AAGAGAGCCA GTTCACGACA 
GTCACCCCAG TTAAGAAGCC CAAAACACCA ACAAATACAT GTGAACTGAC AGCAACTGAG 
GTTCTTACTG AGAAAAGGTG TCATTCTGTG GATTTAGATC TCAACCGAGG TCATGTGGAT 
AGCAGCCAAA AGAAGAGAAA GGCCAAACTA TTTGGAAGTC TCGAAAGAGG CCTAGATAAA 
GTGATTACAA TGCTTACACC AGGCAAAAAG AAGCGATTCA GTAGACCTGG GCCAAGAAAA 
CTTAAGGCGC ACTATAATGT TACCATGACT CAACTACTGA ATCCAGACCA ACTGCTGAAT 
GAAATAATCT CTATCCTTTC AAAGAAGCAA GTGGAATACG TTAAGAAGGG CTACACCCTG 
AAATGCCAAA CAAAGCCAGA TTTTGGCAGA GAATCTATGA AGTTTGAGTT AGAAGTGTGT 
CGGCTCAGTA AGAATGGAGC GGTTGGTATC AGGAGGCAGA GGCTTAGAGG GGATGCTTGG 
GTCTACAAGA GGCTGGTGGA AGACATTTTA TCCAGCTGCC AAGTGTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_015136280.1
CDS174..1961
Translation

Target ORF information:

RefSeq Version XM_015280794.1
Organism Gallus gallus(chicken)
Definition Gallus gallus maternal embryonic leucine zipper kinase (MELK), transcript variant X1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 XM_015280794.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
ATGTCTGTCA GTGACTACGA GGAAATTCTC AAGTACTATG AATTACATGA AACAATTGGA 
ACGGGTGGTT TTGCAAAGGT AAAACTCGGA CGGCATCTTC TTACTGGTGA AAAAGTTGCA 
ATAAAAATCA TGGACAAAGT TGCTTTAGGG GATGACTTGC CTCGAGTTAA AACAGAAATT 
GATGCCATGA AGAACTTAAG TCATCAACAT GTTTGCCGAT TGTACCATGT AATAGAGACA 
TCAAAGAAAA TATTCATGGT CTTAGAGTAT TGTCCCGGAG GAGAGCTGTT TGATTACATT 
GTTTCTAAGG ACCGCCTTTC GGAAGAGGAA GCTCGTGTCT TTTTTCGACA GATTGTTTCA 
GCAATTGCTT ATGTTCACAG TCAGGGTTAT GCCCACAGAG ACCTCAAACC AGAAAATCTG 
CTGATTGATG AGGAACATAA TTTGAAGCTG ATAGACTTCG GACTCTGTGC AAAGCCAAAG 
GGAGGCCTCG ATTACCGCCT GAACACATGC TGTGGAAGCC CAGCATACGC AGCACCAGAG 
CTAATCCAGG GCAAAGCGTA TATTGGCTCA GAGGCAGATA TTTGGAGCAT GGGTGTACTG 
CTGTATGCTC TGCTGTGTGG GTTTCTTCCT TTTGATGATG ACAATGTGAT GGCACTCTAC 
AGGAGGATAA CAAGAGGAAA ATACGCTGTT CCAAAATGGC TTTCACCTAG CAGTACGCTG 
CTGCTTAACC AACTGCTACA GTGGAATTAC GACCAGATGT CAGCAACCTA TCTCTTGCTT 
CAATCCAAGA AGTTTCGTGG CAAGCGCATT CACTTAAGAA TTCCATCACA AACAGGACAT 
GCCAGTACAA CACAGTCAGC AAGCCTGGGG TCTGAAAAGG CTATGACATA TGAAGATGTG 
CCAGACTGCA GCGATGTGCC AGATGCGTTT GGATCTATGG AATTCTCTGA TGCAGCTTCA 
CTGTTTGAGG AATCTCCACT AGAAGAGTTT AGTCTAAATA CTCACAGAAC AAAGCAGCAT 
TCGAGGCATG ATACTCAACT GGGTGATATG GAATTCACAC TGTCAACTCC AGTGACAAGA 
AAAGTGGCAT CGAAGAAGCA TGCAAACAAA GAGAATGTTG ATGCAGAGTC AGCATTAAGA 
AATGAGCTGC CCTTTGCACT TCCTGCTCCA AAGGCCTCCT TTTCAAAGAG TCAGACAGAG 
AAGCAAGTAC AAGGCATACC CTTTCAGGCA CCTACTTCCA AAGAGAGCCA GTTCACGACA 
GTCACCCCAG TTAAGAAGCC CAAAACACCA ACAAATACAT GTGAACTGAC AGCAACTGAG 
GTTCTTACTG AGAAAAGGTG TCATTCTGTG GATTTAGATC TCAACCGAGG TCATGTGGAT 
AGCAGCCAAA AGAAGAGAAA GGCCAAACTA TTTGGAAGTC TCGAAAGAGG CCTAGATAAA 
GTGATTACAA TGCTTACACC AGGCAAAAAG AAGCGATTCA GTAGACCTGG GCCAAGAAAA 
CTTAAGGCGC ACTATAATGT TACCATGACT CAACTACTGA ATCCAGACCA ACTGCTGAAT 
GAAATAATCT CTATCCTTTC AAAGAAGCAA GTGGAATACG TTAAGAAGGG CTACACCCTG 
AAATGCCAAA CAAAGCCAGA TTTTGGCAGA GAATCTATGA AGTTTGAGTT AGAAGTGTGT 
CGGCTCAGTA AGAATGGAGC GGTTGGTATC AGGAGGCAGA GGCTTAGAGG GGATGCTTGG 
GTCTACAAGA GGCTGGTGGA AGACATTTTA TCCAGCTGCC AAGTGTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

PubMed

book

Full-length cDNAs from chicken bursal lymphocytes to facilitate gene function analysis.
Genome biology6(1)R6(2005)
Caldwell RB,Kierzek AM,Arakawa H,Bezzubov Y,Zaim J,Fiedler P,Kutter S,Blagodatski A,Kostovska D,Koter M,Plachy J,Carninci P,Hayashizaki Y,Buerstedde JM