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KLLN cDNA ORF clone, Homo sapiens(Human)

Gene Symbol KLLN
Entrez Gene ID 100144748
Full Name killin, p53-regulated DNA replication inhibitor
Synonyms CWS4,KILLIN
General protein information
Preferred Names

killin, p53-regulated DNA replication inhibitor

Names

killin

Gene Type protein-coding
Organism Homo sapiens(Human)
Genome

10

10q23.31

Summary The protein encoded by this intronless gene is found in the nucleus, where it can inhibit DNA synthesis and promote S phase arrest coupled to apoptosis. The expression of this DNA binding protein is upregulated by transcription factor p53. [provided by RefSeq, Dec 2012].
Disorder MIM:

612105


mRNA and Protein(s)

mRNA Protein Name
NM_001126049.1 NP_001119521.1 killin



Macaca mulatta (rhesus monkey) LOC100426503 XP_002805787.1
Pan troglodytes (chimpanzee) KLLN XP_003312729.1
Homo sapiens (human) KLLN NP_001119521.1


Related articles in PubMed

KLLN epigenotype-phenotype associations in Cowden syndrome.
Nizialek EA, Mester JL, Dhiman VK, Smiraglia DJ, Eng C
European journal of human genetics : EJHG23(11)1538-43(2015 Nov)

Cell cycle specific distribution of killin: evidence for negative regulation of both DNA and RNA synthesis.
Qiao M, Luo D, Kuang Y, Feng H, Luo G, Liang P
Cell cycle (Georgetown, Tex.)14(12)1823-9(2015)

Nuclear KLLN expression associates with improved relapse-free survival for prostate carcinoma.
Wang Y, Roma A, Nolley R, Abdul-Karim F, Peehl DM, Eng C
Endocrine-related cancer21(4)579-86(2014 Aug)

Activation of AR sensitizes breast carcinomas to NVP-BEZ235's therapeutic effect mediated by PTEN and KLLN upregulation.
Wang Y, Yu Q, He X, Romigh T, Altemus J, Eng C
Molecular cancer therapeutics13(2)517-27(2014 Feb)

Germline and somatic KLLN alterations in breast cancer dysregulate G2 arrest.
Nizialek EA, Peterson C, Mester JL, Downes-Kelly E, Eng C
Human molecular genetics22(12)2451-61(2013 Jun)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

overexpression of microRNA-224 enhanced the proliferation abilities of HO8910 and knockdown of microRNA-224 suppressed the proliferation abilities of HO8910PM by KLLN-cyclin A pathway.
Title: Upregulated microRNA-224 promotes ovarian cancer cell proliferation by targeting KLLN.

The results suggest a critical role for KLLN as a potential regulator of pericentric heterochromatin formation, genomic stability and gene expression.
Title: Cancer-predisposition gene KLLN maintains pericentric H3K9 trimethylation protecting genomic stability.

Germline promoter hypermethylation of KLLN is associated with particular malignant and benign Cowden syndrome features, which is dependent on the PTEN mutation status.
Title: KLLN epigenotype-phenotype associations in Cowden syndrome.

Killin has a role in competitively inhibiting the formation of DNA replication forks during S-phase, as well as potentially negatively regulate RNA synthesis during other cell cycle phases
Title: Cell cycle specific distribution of killin: evidence for negative regulation of both DNA and RNA synthesis.

Nuclear KLLN expression associates with improved relapse-free survival for prostate carcinoma
Title: Nuclear KLLN expression associates with improved relapse-free survival for prostate carcinoma.

The following KLLN gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the KLLN cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

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***CloneID RefSeq Accession Definition **Vector *Turnaround time Price (USD) Select
OHu18226
NM_001126049.1
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Homo sapiens killin, p53-regulated DNA replication inhibitor (KLLN), mRNA. pcDNA3.1+/C-(K)DYK or customized vector
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.


CloneID OHu18226
Clone ID Related Accession (Same CDS sequence) NM_001126049.1
Accession Version NM_001126049.1 Latest version! Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 537bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2017-05-03
Organism Homo sapiens(Human)
Product killin
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from EU552092.1. This sequence is a reference standard in the RefSeqGene project. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript is intronless :: EU552092.1 [ECO:0000345] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
ATGGATCGCC CGGGGCCAGG CTCCGCGCGC CCCGGCCGGA CCGTGCACGT TTGGGGTTAC 
CGGGTTGAGT GGAAAGTACG GAACGGTAGG AAGCTGCAGC CCAGCGAGTG GGCGGGGCGA
GGAGACCTAG GAGGGTTCAA AAGGAGGTGG AAGGATACAC GGGCCACAGT CGGAACTACT
TTCCGAAGGA GGTCACGTGT GTCCCTAGTT GGGGAACTTT CCAAATTCCC ACTCCCCAGT
GATAGCTCCG GAGGCAAGTC GTCTTCTTCC TTTGCTCGGG GTGCTCTTGC CTGGTGCAGG
CAGCGGAACC CCAACCCTTC CTGCGCCGCG GCGGAAACAG GGGCTCGGAC CAGCCTCCCG
AAGGAGCGCT GTCGGGGCTG GCGCTTGGGG AACTGGTTAC ACAAGCACCC ACATCCAAAC
ACGTGCCCCC GCCTCCCCGC CTGCTGGCTG CCGCCGATTC TTACAGAACG CGGGGAGAGA
GTCCCCAAAC TGGTGCCACT CCTCGCCTGC TACCCTAAGA GCAAGCCAAA GGACTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001119521.1
CDS951..1487
Misc Feature(1)936..938
Exon (1)1..4277
Translation

Target ORF information:

RefSeq Version NM_001126049.1
Organism Homo sapiens(Human)
Definition Homo sapiens killin, p53-regulated DNA replication inhibitor (KLLN), mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_001126049.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
ATGGATCGCC CGGGGCCAGG CTCCGCGCGC CCCGGCCGGA CCGTGCACGT TTGGGGTTAC 
CGGGTTGAGT GGAAAGTACG GAACGGTAGG AAGCTGCAGC CCAGCGAGTG GGCGGGGCGA
GGAGACCTAG GAGGGTTCAA AAGGAGGTGG AAGGATACAC GGGCCACAGT CGGAACTACT
TTCCGAAGGA GGTCACGTGT GTCCCTAGTT GGGGAACTTT CCAAATTCCC ACTCCCCAGT
GATAGCTCCG GAGGCAAGTC GTCTTCTTCC TTTGCTCGGG GTGCTCTTGC CTGGTGCAGG
CAGCGGAACC CCAACCCTTC CTGCGCCGCG GCGGAAACAG GGGCTCGGAC CAGCCTCCCG
AAGGAGCGCT GTCGGGGCTG GCGCTTGGGG AACTGGTTAC ACAAGCACCC ACATCCAAAC
ACGTGCCCCC GCCTCCCCGC CTGCTGGCTG CCGCCGATTC TTACAGAACG CGGGGAGAGA
GTCCCCAAAC TGGTGCCACT CCTCGCCTGC TACCCTAAGA GCAAGCCAAA GGACTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

book

KLLN epigenotype-phenotype associations in Cowden syndrome.
European journal of human genetics : EJHG23(11)1538-43(2015 Nov)
Nizialek EA,Mester JL,Dhiman VK,Smiraglia DJ,Eng C


book

Cell cycle specific distribution of killin: evidence for negative regulation of both DNA and RNA synthesis.
Cell cycle (Georgetown, Tex.)14(12)1823-9(2015)
Qiao M,Luo D,Kuang Y,Feng H,Luo G,Liang P


book

Nuclear KLLN expression associates with improved relapse-free survival for prostate carcinoma.
Endocrine-related cancer21(4)579-86(2014 Aug)
Wang Y,Roma A,Nolley R,Abdul-Karim F,Peehl DM,Eng C


book

Activation of AR sensitizes breast carcinomas to NVP-BEZ235's therapeutic effect mediated by PTEN and KLLN upregulation.
Molecular cancer therapeutics13(2)517-27(2014 Feb)
Wang Y,Yu Q,He X,Romigh T,Altemus J,Eng C


book

Germline and somatic KLLN alterations in breast cancer dysregulate G2 arrest.
Human molecular genetics22(12)2451-61(2013 Jun)
Nizialek EA,Peterson C,Mester JL,Downes-Kelly E,Eng C


 
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