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RACK1 cDNA ORF clone, Homo sapiens(Human)

Gene Symbol RACK1
Entrez Gene ID 10399
Full Name receptor for activated C kinase 1
Synonyms GNB2L1,Gnb2-rs1,H12.3,HLC-7,PIG21
General protein information
Preferred Names

receptor for activated C kinase 1

Names

receptor of activated protein C kinase 1
cell proliferation-inducing gene 21 protein
guanine nucleotide binding protein (G protein), beta polypeptide 2-like 1
guanine nucleotide binding protein beta polypeptide 2-like 1
guanine nucleotide-binding protein subunit beta-2-like 1
guanine nucleotide-binding protein subunit beta-like protein 12.3
human lung cancer oncogene 7 protein
lung cancer oncogene 7
proliferation-inducing gene 21
protein homologous to chicken B complex protein, guanine nucleotide binding
receptor of activated protein kinase C 1
small ribosomal subunit protein RACK1

Gene Type protein-coding
Organism Homo sapiens(Human)
Genome

5

5q35.3

Disorder MIM:

176981


mRNA and Protein(s)

mRNA Protein Name
NM_006098.4 NP_006089.1 receptor of activated protein C kinase 1



Macaca mulatta (rhesus monkey) LOC708526 XP_001105066.1
Homo sapiens (human) GNB2L1 NP_006089.1
Pan troglodytes (chimpanzee) GNB2L1 XP_518165.3
Caenorhabditis elegans (roundworm) rack-1 NP_501859.1
Kluyveromyces lactis KLLA0E12277g XP_454502.1
Schizosaccharomyces pombe (fission yeast) cpc2 NP_593770.1
Arabidopsis thaliana (thale cress) ATARCA NP_173248.1
Bos taurus (cattle) GNB2L1 XP_005209294.1
Mus musculus (house mouse) Gnb2l1 NP_032169.1
Rattus norvegicus (Norway rat) Gnb2l1 NP_570090.2
Magnaporthe oryzae (rice blast fungus) MGG_04719 XP_003710816.1
Arabidopsis thaliana (thale cress) RACK1B_AT NP_175296.1
Gallus gallus (chicken) GNB2L1 NP_001004378.1
Drosophila melanogaster (fruit fly) Rack1 NP_001260218.1
Saccharomyces cerevisiae (baker's yeast) ASC1 NP_013834.1
Eremothecium gossypii AGOS_AFR199C NP_985746.1
Arabidopsis thaliana (thale cress) RACK1C_AT NP_188441.1
Oryza sativa (rice) Os01g0686800 NP_001043910.1
Oryza sativa (rice) Os05g0552300 NP_001056254.1
Canis lupus familiaris (dog) GNB2L1 XP_005626337.1
Danio rerio (zebrafish) gnb2l1 NP_571519.1
Anopheles gambiae (African malaria mosquito) AgaP_AGAP010173 XP_319347.2
Neurospora crassa NCU05810 XP_960070.1
Xenopus tropicalis (tropical clawed frog) gnb2l1 NP_001004946.1


Related articles in PubMed

ZNF598 and RACK1 Regulate Mammalian Ribosome-Associated Quality Control Function by Mediating Regulatory 40S Ribosomal Ubiquitylation.
Sundaramoorthy E, Leonard M, Mak R, Liao J, Fulzele A, Bennett EJ
Molecular cell65(4)751-760.e4(2017 Feb)

TAR DNA-binding protein 43 inhibits inflammatory response and protects chondrocyte function by modulating RACK1 expression in osteoarthritis.
Huang Y, Huang Q, Su H, Mai X, Feng E, Cao Z, Zeng X
Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie85362-371(2017 Jan)

Rack1 Mediates the Interaction of P-Glycoprotein with Anxa2 and Regulates Migration and Invasion of Multidrug-Resistant Breast Cancer Cells.
Yang Y, Wu N, Wang Z, Zhang F, Tian R, Ji W, Ren X, Niu R
International journal of molecular sciences17(10)(2016 Oct)

RACK1 Is an Interaction Partner of ATG5 and a Novel Regulator of Autophagy.
Erbil S, Oral O, Mitou G, Kig C, Durmaz-Timucin E, Guven-Maiorov E, Gulacti F, Gokce G, Dengjel J, Sezerman OU, Gozuacik D
The Journal of biological chemistry291(32)16753-65(2016 Aug)

Receptor for activated protein kinase C 1 suppresses gastric tumor progression through nuclear factor-kB pathway.
Yong-Zheng X, Wan-Li M, Ji-Ming M, Xue-Qun R
Indian journal of cancer52 Suppl 3E172-5(2015 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

RACK1 could be a relevant target of endocrine disrupting chemicals, responding in opposite ways to androgen receptor agonists/antagonists, representing a bridge between the endocrine system and the innate immune system.
Title: The scaffold protein RACK1 is a target of endocrine disrupting chemicals (EDCs) with important implication in immunity.

results from this study suggest that TDP-43 represents a translational repressor not only for specific mRNAs but for overall translation and that its binding to polyribosomes through RACK1 may promote, under conditions inducingAmyotrophic Lateral Sclerosis pathogenesis, the formation of cytoplasmic inclusions.
Title: Increased cytoplasmic TDP-43 reduces global protein synthesis by interacting with RACK1 on polyribosomes.

ZNF598, RACK1, and 40S regulatory ubiquitylation plays a pivotal role in mammalian ribosome-associated quality control pathways.
Title: ZNF598 and RACK1 Regulate Mammalian Ribosome-Associated Quality Control Function by Mediating Regulatory 40S Ribosomal Ubiquitylation.

The data indicated that TDP-43/p35 contributed to stress granule formation by promoting RACK1 expression. The study sheds new light on post-transcriptional regulation and apoptosis in osteoarthritis (OA) by RACK1, which is a potential treatment strategy for OA.
Title: TAR DNA-binding protein 43 inhibits inflammatory response and protects chondrocyte function by modulating RACK1 expression in osteoarthritis.

Rack1 regulated P-Glycoprotein activity, which was necessary for adriamycin-induced P-Glycoprotein - mediated phosphorylation of Anxa2 and Erk1/2.
Title: Rack1 Mediates the Interaction of P-Glycoprotein with Anxa2 and Regulates Migration and Invasion of Multidrug-Resistant Breast Cancer Cells.

The following RACK1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the RACK1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

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***CloneID RefSeq Accession Definition **Vector *Turnaround time Price (USD) Select
OHu15971
NM_006098.4
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Homo sapiens receptor for activated C kinase 1 (RACK1), mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
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$309.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.


CloneID OHu15971
Clone ID Related Accession (Same CDS sequence) NM_006098.4
Accession Version NM_006098.4 Latest version! Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 954bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2017-05-07
Organism Homo sapiens(Human)
Product receptor of activated protein C kinase 1
Comment VALIDATED REFSEQ: This record has undergone validation or preliminary review. This record has been curated by NCBI staff in collaboration with Francesco Amaldi. The reference sequence was derived from AK222488.1, M24194.1 and BC029996.1. On Dec 14, 2005 this sequence version replaced NM_006098.3. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1163658.144897.1, SRR1163655.130391.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968832, SAMEA2142348 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: full length.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
ATGACTGAGC AGATGACCCT TCGTGGCACC CTCAAGGGCC ACAACGGCTG GGTAACCCAG 
ATCGCTACTA CCCCGCAGTT CCCGGACATG ATCCTCTCCG CCTCTCGAGA TAAGACCATC
ATCATGTGGA AACTGACCAG GGATGAGACC AACTATGGAA TTCCACAGCG TGCTCTGCGG
GGTCACTCCC ACTTTGTTAG TGATGTGGTT ATCTCCTCAG ATGGCCAGTT TGCCCTCTCA
GGCTCCTGGG ATGGAACCCT GCGCCTCTGG GATCTCACAA CGGGCACCAC CACGAGGCGA
TTTGTGGGCC ATACCAAGGA TGTGCTGAGT GTGGCCTTCT CCTCTGACAA CCGGCAGATT
GTCTCTGGAT CTCGAGATAA AACCATCAAG CTATGGAATA CCCTGGGTGT GTGCAAATAC
ACTGTCCAGG ATGAGAGCCA CTCAGAGTGG GTGTCTTGTG TCCGCTTCTC GCCCAACAGC
AGCAACCCTA TCATCGTCTC CTGTGGCTGG GACAAGCTGG TCAAGGTATG GAACCTGGCT
AACTGCAAGC TGAAGACCAA CCACATTGGC CACACAGGCT ATCTGAACAC GGTGACTGTC
TCTCCAGATG GATCCCTCTG TGCTTCTGGA GGCAAGGATG GCCAGGCCAT GTTATGGGAT
CTCAACGAAG GCAAACACCT TTACACGCTA GATGGTGGGG ACATCATCAA CGCCCTGTGC
TTCAGCCCTA ACCGCTACTG GCTGTGTGCT GCCACAGGCC CCAGCATCAA GATCTGGGAT
TTAGAGGGAA AGATCATTGT AGATGAACTG AAGCAAGAAG TTATCAGTAC CAGCAGCAAG
GCAGAACCAC CCCAGTGCAC CTCCCTGGCC TGGTCTGCTG ATGGCCAGAC TCTGTTTGCT
GGCTACACGG ACAACCTGGT GCGAGTGTGG CAGGTGACCA TTGGCACACG CTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_006089.1
CDS107..1060
Misc Feature(1)1
Misc Feature(2)3
Misc Feature(3)107..109
Misc Feature(4)<110..1036
Misc Feature(5)110..112
Misc Feature(6)122..124
Misc Feature(7)125..1039
Misc Feature(8)134..136
Misc Feature(9)143..238
Misc Feature(10)order(146..148,203..205,215..217,233..238,287..292,344..346,356..358,374..379,413..418,467..469,482..484,500..505,545..547,602..604,617..619,635..640,674..679,731..733,743..745,761..766,800..802,851..853,881..886,947..952,1004..1006,1016..1018,1034..1039)
Misc Feature(11)158..289
Misc Feature(12)260..262
Misc Feature(13)287..379
Misc Feature(14)305..415
Misc Feature(15)392..394
Misc Feature(16)413..505
Misc Feature(17)428..538
Misc Feature(18)494..496
Misc Feature(19)542..640
Misc Feature(20)560..673
Misc Feature(21)653..655
Misc Feature(22)674..766
Misc Feature(23)689..799
Misc Feature(24)788..790
Misc Feature(25)788..790
Misc Feature(26)797..886
Misc Feature(27)812..943
Misc Feature(28)932..934
Misc Feature(29)947..1039
Misc Feature(30)962..1036
Misc Feature(31)1052..1054
Exon (1)1..215
Exon (2)216..387
Exon (3)388..535
Exon (4)536..631
Exon (5)632..742
Exon (6)743..883
Exon (7)884..994
Exon (8)995..1109
Translation

Target ORF information:

RefSeq Version NM_006098.4
Organism Homo sapiens(Human)
Definition Homo sapiens receptor for activated C kinase 1 (RACK1), mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_006098.4

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
ATGACTGAGC AGATGACCCT TCGTGGCACC CTCAAGGGCC ACAACGGCTG GGTAACCCAG 
ATCGCTACTA CCCCGCAGTT CCCGGACATG ATCCTCTCCG CCTCTCGAGA TAAGACCATC
ATCATGTGGA AACTGACCAG GGATGAGACC AACTATGGAA TTCCACAGCG TGCTCTGCGG
GGTCACTCCC ACTTTGTTAG TGATGTGGTT ATCTCCTCAG ATGGCCAGTT TGCCCTCTCA
GGCTCCTGGG ATGGAACCCT GCGCCTCTGG GATCTCACAA CGGGCACCAC CACGAGGCGA
TTTGTGGGCC ATACCAAGGA TGTGCTGAGT GTGGCCTTCT CCTCTGACAA CCGGCAGATT
GTCTCTGGAT CTCGAGATAA AACCATCAAG CTATGGAATA CCCTGGGTGT GTGCAAATAC
ACTGTCCAGG ATGAGAGCCA CTCAGAGTGG GTGTCTTGTG TCCGCTTCTC GCCCAACAGC
AGCAACCCTA TCATCGTCTC CTGTGGCTGG GACAAGCTGG TCAAGGTATG GAACCTGGCT
AACTGCAAGC TGAAGACCAA CCACATTGGC CACACAGGCT ATCTGAACAC GGTGACTGTC
TCTCCAGATG GATCCCTCTG TGCTTCTGGA GGCAAGGATG GCCAGGCCAT GTTATGGGAT
CTCAACGAAG GCAAACACCT TTACACGCTA GATGGTGGGG ACATCATCAA CGCCCTGTGC
TTCAGCCCTA ACCGCTACTG GCTGTGTGCT GCCACAGGCC CCAGCATCAA GATCTGGGAT
TTAGAGGGAA AGATCATTGT AGATGAACTG AAGCAAGAAG TTATCAGTAC CAGCAGCAAG
GCAGAACCAC CCCAGTGCAC CTCCCTGGCC TGGTCTGCTG ATGGCCAGAC TCTGTTTGCT
GGCTACACGG ACAACCTGGT GCGAGTGTGG CAGGTGACCA TTGGCACACG CTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

book

ZNF598 and RACK1 Regulate Mammalian Ribosome-Associated Quality Control Function by Mediating Regulatory 40S Ribosomal Ubiquitylation.
Molecular cell65(4)751-760.e4(2017 Feb)
Sundaramoorthy E,Leonard M,Mak R,Liao J,Fulzele A,Bennett EJ


book

TAR DNA-binding protein 43 inhibits inflammatory response and protects chondrocyte function by modulating RACK1 expression in osteoarthritis.
Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie85362-371(2017 Jan)
Huang Y,Huang Q,Su H,Mai X,Feng E,Cao Z,Zeng X


book

Rack1 Mediates the Interaction of P-Glycoprotein with Anxa2 and Regulates Migration and Invasion of Multidrug-Resistant Breast Cancer Cells.
International journal of molecular sciences17(10)(2016 Oct)
Yang Y,Wu N,Wang Z,Zhang F,Tian R,Ji W,Ren X,Niu R


book

RACK1 Is an Interaction Partner of ATG5 and a Novel Regulator of Autophagy.
The Journal of biological chemistry291(32)16753-65(2016 Aug)
Erbil S,Oral O,Mitou G,Kig C,Durmaz-Timucin E,Guven-Maiorov E,Gulacti F,Gokce G,Dengjel J,Sezerman OU,Gozuacik D


book

Receptor for activated protein kinase C 1 suppresses gastric tumor progression through nuclear factor-kB pathway.
Indian journal of cancer52 Suppl 3E172-5(2015 Dec)
Yong-Zheng X,Wan-Li M,Ji-Ming M,Xue-Qun R


 
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