CHRNA2 cDNA ORF clone, Homo sapiens(human)

The following CHRNA2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the CHRNA2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

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***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OHu17978 NM_000742.4
Latest version!
Homo sapiens cholinergic receptor nicotinic alpha 2 subunit (CHRNA2), transcript variant 1, mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK
$189.50-$265.30
$379.00
OHu17978 XM_011544388.1
Latest version!
Homo sapiens cholinergic receptor nicotinic alpha 2 subunit (CHRNA2), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK
$189.50-$265.30
$379.00
OHu17978 XM_006716282.1
Latest version!
Homo sapiens cholinergic receptor nicotinic alpha 2 subunit (CHRNA2), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK
$189.50-$265.30
$379.00
OHu41986 NM_001347705.2
Latest version!
Homo sapiens cholinergic receptor nicotinic alpha 2 subunit (CHRNA2), transcript variant 3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 5-7 $223.30
$319.00
OHu41986 NM_001347706.2
Latest version!
Homo sapiens cholinergic receptor nicotinic alpha 2 subunit (CHRNA2), transcript variant 4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 5-7 $223.30
$319.00
OHu56878 NM_001347708.2
Latest version!
Homo sapiens cholinergic receptor nicotinic alpha 2 subunit (CHRNA2), transcript variant 6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 5-7 $139.30
$199.00
OHu56878 NM_001347707.2
Latest version!
Homo sapiens cholinergic receptor nicotinic alpha 2 subunit (CHRNA2), transcript variant 5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 5-7 $139.30
$199.00
OHu56878 XM_011544389.2
Latest version!
Homo sapiens cholinergic receptor nicotinic alpha 2 subunit (CHRNA2), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 5-7 $139.30
$199.00
OHu22359 NM_001282455.2
Latest version!
Homo sapiens cholinergic receptor nicotinic alpha 2 subunit (CHRNA2), transcript variant 2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $307.30
$439.00
View Old Accession Versions >>
OHu56878 XM_011544389.1 Homo sapiens cholinergic receptor nicotinic alpha 2 subunit (CHRNA2), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 5-7 $139.30
$199.00
OHu41986 NM_001347706.1 Homo sapiens cholinergic receptor nicotinic alpha 2 subunit (CHRNA2), transcript variant 4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 5-7 $223.30
$319.00
OHu17978 NM_000742.3 Homo sapiens cholinergic receptor nicotinic alpha 2 subunit (CHRNA2), transcript variant 1, mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK
$189.50-$265.30
$379.00
OHu41986 NM_001347705.1 Homo sapiens cholinergic receptor nicotinic alpha 2 subunit (CHRNA2), transcript variant 3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 5-7 $223.30
$319.00
OHu56878 NM_001347708.1 Homo sapiens cholinergic receptor nicotinic alpha 2 subunit (CHRNA2), transcript variant 6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 5-7 $139.30
$199.00
OHu56878 NM_001347707.1 Homo sapiens cholinergic receptor nicotinic alpha 2 subunit (CHRNA2), transcript variant 5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 5-7 $139.30
$199.00
OHu22359 NM_001282455.1 Homo sapiens cholinergic receptor nicotinic alpha 2 subunit (CHRNA2), transcript variant 2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $307.30
$439.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OHu17978
    Clone ID Related Accession (Same CDS sequence) NM_000742.3 , XM_006716282.1 , NM_000742.4 , XM_011544388.1
    Accession Version NM_000742.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1590bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-04-29
    Organism Homo sapiens(human)
    Product neuronal acetylcholine receptor subunit alpha-2 isoform 1 precursor
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA407151.1, U62431.1, DA096824.1, CD013901.1, AF311103.5 and AW292180.1. This sequence is a reference standard in the RefSeqGene project. On Sep 11, 2007 this sequence version replaced NM_000742.2. Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803613.124252.1, SRR1660809.23572.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2145743, SAMEA2153427 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    ATGGGCCCCT CCTGTCCTGT GTTCCTGTCC TTCACAAAGC TCAGCCTGTG GTGGCTCCTT 
    CTGACCCCAG CAGGTGGAGA GGAAGCTAAG CGCCCACCTC CCAGGGCTCC TGGAGACCCA
    CTCTCCTCTC CCAGTCCCAC GGCATTGCCG CAGGGAGGCT CGCATACCGA GACTGAGGAC
    CGGCTCTTCA AACACCTCTT CCGGGGCTAC AACCGCTGGG CGCGCCCGGT GCCCAACACT
    TCAGACGTGG TGATTGTGCG CTTTGGACTG TCCATCGCTC AGCTCATCGA TGTGGATGAG
    AAGAACCAAA TGATGACCAC CAACGTCTGG CTAAAACAGG AGTGGAGCGA CTACAAACTG
    CGCTGGAACC CCACTGATTT TGGCAACATC ACATCTCTCA GGGTCCCTTC TGAGATGATC
    TGGATCCCCG ACATTGTTCT CTACAACAAT GCAGATGGGG AGTTTGCAGT GACCCACATG
    ACCAAGGCCC ACCTCTTCTC CACGGGCACT GTGCACTGGG TGCCCCCGGC CATCTACAAG
    AGCTCCTGCA GCATCGACGT CACCTTCTTC CCCTTCGACC AGCAGAACTG CAAGATGAAG
    TTTGGCTCCT GGACTTATGA CAAGGCCAAG ATCGACCTGG AGCAGATGGA GCAGACTGTG
    GACCTGAAGG ACTACTGGGA GAGCGGCGAG TGGGCCATCG TCAATGCCAC GGGCACCTAC
    AACAGCAAGA AGTACGACTG CTGCGCCGAG ATCTACCCCG ACGTCACCTA CGCCTTCGTC
    ATCCGGCGGC TGCCGCTCTT CTACACCATC AACCTCATCA TCCCCTGCCT GCTCATCTCC
    TGCCTCACTG TGCTGGTCTT CTACCTGCCC TCCGACTGCG GCGAGAAGAT CACGCTGTGC
    ATTTCGGTGC TGCTGTCACT CACCGTCTTC CTGCTGCTCA TCACTGAGAT CATCCCGTCC
    ACCTCGCTGG TCATCCCGCT CATCGGCGAG TACCTGCTGT TCACCATGAT CTTCGTCACC
    CTGTCCATCG TCATCACCGT CTTCGTGCTC AATGTGCACC ACCGCTCCCC CAGCACCCAC
    ACCATGCCCC ACTGGGTGCG GGGGGCCCTT CTGGGCTGTG TGCCCCGGTG GCTTCTGATG
    AACCGGCCCC CACCACCCGT GGAGCTCTGC CACCCCCTAC GCCTGAAGCT CAGCCCCTCT
    TATCACTGGC TGGAGAGCAA CGTGGATGCC GAGGAGAGGG AGGTGGTGGT GGAGGAGGAG
    GACAGATGGG CATGTGCAGG TCATGTGGCC CCCTCTGTGG GCACCCTCTG CAGCCACGGC
    CACCTGCACT CTGGGGCCTC AGGTCCCAAG GCTGAGGCTC TGCTGCAGGA GGGTGAGCTG
    CTGCTATCAC CCCACATGCA GAAGGCACTG GAAGGTGTGC ACTACATTGC CGACCACCTG
    CGGTCTGAGG ATGCTGACTC TTCGGTGAAG GAGGACTGGA AGTATGTTGC CATGGTCATC
    GACAGGATCT TCCTCTGGCT GTTTATCATC GTCTGCTTCC TGGGGACCAT CGGCCTCTTT
    CTGCCTCCGT TCCTAGCTGG AATGATCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_000733.2
    CDS610..2199
    Translation

    Target ORF information:

    RefSeq Version NM_000742.3
    Organism Homo sapiens(human)
    Definition Homo sapiens cholinergic receptor nicotinic alpha 2 subunit (CHRNA2), transcript variant 1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_000742.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    ATGGGCCCCT CCTGTCCTGT GTTCCTGTCC TTCACAAAGC TCAGCCTGTG GTGGCTCCTT 
    CTGACCCCAG CAGGTGGAGA GGAAGCTAAG CGCCCACCTC CCAGGGCTCC TGGAGACCCA
    CTCTCCTCTC CCAGTCCCAC GGCATTGCCG CAGGGAGGCT CGCATACCGA GACTGAGGAC
    CGGCTCTTCA AACACCTCTT CCGGGGCTAC AACCGCTGGG CGCGCCCGGT GCCCAACACT
    TCAGACGTGG TGATTGTGCG CTTTGGACTG TCCATCGCTC AGCTCATCGA TGTGGATGAG
    AAGAACCAAA TGATGACCAC CAACGTCTGG CTAAAACAGG AGTGGAGCGA CTACAAACTG
    CGCTGGAACC CCACTGATTT TGGCAACATC ACATCTCTCA GGGTCCCTTC TGAGATGATC
    TGGATCCCCG ACATTGTTCT CTACAACAAT GCAGATGGGG AGTTTGCAGT GACCCACATG
    ACCAAGGCCC ACCTCTTCTC CACGGGCACT GTGCACTGGG TGCCCCCGGC CATCTACAAG
    AGCTCCTGCA GCATCGACGT CACCTTCTTC CCCTTCGACC AGCAGAACTG CAAGATGAAG
    TTTGGCTCCT GGACTTATGA CAAGGCCAAG ATCGACCTGG AGCAGATGGA GCAGACTGTG
    GACCTGAAGG ACTACTGGGA GAGCGGCGAG TGGGCCATCG TCAATGCCAC GGGCACCTAC
    AACAGCAAGA AGTACGACTG CTGCGCCGAG ATCTACCCCG ACGTCACCTA CGCCTTCGTC
    ATCCGGCGGC TGCCGCTCTT CTACACCATC AACCTCATCA TCCCCTGCCT GCTCATCTCC
    TGCCTCACTG TGCTGGTCTT CTACCTGCCC TCCGACTGCG GCGAGAAGAT CACGCTGTGC
    ATTTCGGTGC TGCTGTCACT CACCGTCTTC CTGCTGCTCA TCACTGAGAT CATCCCGTCC
    ACCTCGCTGG TCATCCCGCT CATCGGCGAG TACCTGCTGT TCACCATGAT CTTCGTCACC
    CTGTCCATCG TCATCACCGT CTTCGTGCTC AATGTGCACC ACCGCTCCCC CAGCACCCAC
    ACCATGCCCC ACTGGGTGCG GGGGGCCCTT CTGGGCTGTG TGCCCCGGTG GCTTCTGATG
    AACCGGCCCC CACCACCCGT GGAGCTCTGC CACCCCCTAC GCCTGAAGCT CAGCCCCTCT
    TATCACTGGC TGGAGAGCAA CGTGGATGCC GAGGAGAGGG AGGTGGTGGT GGAGGAGGAG
    GACAGATGGG CATGTGCAGG TCATGTGGCC CCCTCTGTGG GCACCCTCTG CAGCCACGGC
    CACCTGCACT CTGGGGCCTC AGGTCCCAAG GCTGAGGCTC TGCTGCAGGA GGGTGAGCTG
    CTGCTATCAC CCCACATGCA GAAGGCACTG GAAGGTGTGC ACTACATTGC CGACCACCTG
    CGGTCTGAGG ATGCTGACTC TTCGGTGAAG GAGGACTGGA AGTATGTTGC CATGGTCATC
    GACAGGATCT TCCTCTGGCT GTTTATCATC GTCTGCTTCC TGGGGACCAT CGGCCTCTTT
    CTGCCTCCGT TCCTAGCTGG AATGATCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu17978
    Clone ID Related Accession (Same CDS sequence) NM_000742.3 , XM_006716282.1 , NM_000742.4 , XM_011544388.1
    Accession Version NM_000742.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1590bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-08-21
    Organism Homo sapiens(human)
    Product neuronal acetylcholine receptor subunit alpha-2 isoform 1 precursor
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA407151.1, U62431.1, DA096824.1, CD013901.1, AF311103.5 and AW292180.1. This sequence is a reference standard in the RefSeqGene project. On Nov 23, 2018 this sequence version replaced NM_000742.3. Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803613.124252.1, SRR1660809.23572.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2145743, SAMEA2153427 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000407991.3/ ENSP00000385026.1 RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: full length.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    ATGGGCCCCT CCTGTCCTGT GTTCCTGTCC TTCACAAAGC TCAGCCTGTG GTGGCTCCTT 
    CTGACCCCAG CAGGTGGAGA GGAAGCTAAG CGCCCACCTC CCAGGGCTCC TGGAGACCCA
    CTCTCCTCTC CCAGTCCCAC GGCATTGCCG CAGGGAGGCT CGCATACCGA GACTGAGGAC
    CGGCTCTTCA AACACCTCTT CCGGGGCTAC AACCGCTGGG CGCGCCCGGT GCCCAACACT
    TCAGACGTGG TGATTGTGCG CTTTGGACTG TCCATCGCTC AGCTCATCGA TGTGGATGAG
    AAGAACCAAA TGATGACCAC CAACGTCTGG CTAAAACAGG AGTGGAGCGA CTACAAACTG
    CGCTGGAACC CCACTGATTT TGGCAACATC ACATCTCTCA GGGTCCCTTC TGAGATGATC
    TGGATCCCCG ACATTGTTCT CTACAACAAT GCAGATGGGG AGTTTGCAGT GACCCACATG
    ACCAAGGCCC ACCTCTTCTC CACGGGCACT GTGCACTGGG TGCCCCCGGC CATCTACAAG
    AGCTCCTGCA GCATCGACGT CACCTTCTTC CCCTTCGACC AGCAGAACTG CAAGATGAAG
    TTTGGCTCCT GGACTTATGA CAAGGCCAAG ATCGACCTGG AGCAGATGGA GCAGACTGTG
    GACCTGAAGG ACTACTGGGA GAGCGGCGAG TGGGCCATCG TCAATGCCAC GGGCACCTAC
    AACAGCAAGA AGTACGACTG CTGCGCCGAG ATCTACCCCG ACGTCACCTA CGCCTTCGTC
    ATCCGGCGGC TGCCGCTCTT CTACACCATC AACCTCATCA TCCCCTGCCT GCTCATCTCC
    TGCCTCACTG TGCTGGTCTT CTACCTGCCC TCCGACTGCG GCGAGAAGAT CACGCTGTGC
    ATTTCGGTGC TGCTGTCACT CACCGTCTTC CTGCTGCTCA TCACTGAGAT CATCCCGTCC
    ACCTCGCTGG TCATCCCGCT CATCGGCGAG TACCTGCTGT TCACCATGAT CTTCGTCACC
    CTGTCCATCG TCATCACCGT CTTCGTGCTC AATGTGCACC ACCGCTCCCC CAGCACCCAC
    ACCATGCCCC ACTGGGTGCG GGGGGCCCTT CTGGGCTGTG TGCCCCGGTG GCTTCTGATG
    AACCGGCCCC CACCACCCGT GGAGCTCTGC CACCCCCTAC GCCTGAAGCT CAGCCCCTCT
    TATCACTGGC TGGAGAGCAA CGTGGATGCC GAGGAGAGGG AGGTGGTGGT GGAGGAGGAG
    GACAGATGGG CATGTGCAGG TCATGTGGCC CCCTCTGTGG GCACCCTCTG CAGCCACGGC
    CACCTGCACT CTGGGGCCTC AGGTCCCAAG GCTGAGGCTC TGCTGCAGGA GGGTGAGCTG
    CTGCTATCAC CCCACATGCA GAAGGCACTG GAAGGTGTGC ACTACATTGC CGACCACCTG
    CGGTCTGAGG ATGCTGACTC TTCGGTGAAG GAGGACTGGA AGTATGTTGC CATGGTCATC
    GACAGGATCT TCCTCTGGCT GTTTATCATC GTCTGCTTCC TGGGGACCAT CGGCCTCTTT
    CTGCCTCCGT TCCTAGCTGG AATGATCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_000733.2
    CDS575..2164
    Translation

    Target ORF information:

    RefSeq Version NM_000742.4
    Organism Homo sapiens(human)
    Definition Homo sapiens cholinergic receptor nicotinic alpha 2 subunit (CHRNA2), transcript variant 1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_000742.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    ATGGGCCCCT CCTGTCCTGT GTTCCTGTCC TTCACAAAGC TCAGCCTGTG GTGGCTCCTT 
    CTGACCCCAG CAGGTGGAGA GGAAGCTAAG CGCCCACCTC CCAGGGCTCC TGGAGACCCA
    CTCTCCTCTC CCAGTCCCAC GGCATTGCCG CAGGGAGGCT CGCATACCGA GACTGAGGAC
    CGGCTCTTCA AACACCTCTT CCGGGGCTAC AACCGCTGGG CGCGCCCGGT GCCCAACACT
    TCAGACGTGG TGATTGTGCG CTTTGGACTG TCCATCGCTC AGCTCATCGA TGTGGATGAG
    AAGAACCAAA TGATGACCAC CAACGTCTGG CTAAAACAGG AGTGGAGCGA CTACAAACTG
    CGCTGGAACC CCACTGATTT TGGCAACATC ACATCTCTCA GGGTCCCTTC TGAGATGATC
    TGGATCCCCG ACATTGTTCT CTACAACAAT GCAGATGGGG AGTTTGCAGT GACCCACATG
    ACCAAGGCCC ACCTCTTCTC CACGGGCACT GTGCACTGGG TGCCCCCGGC CATCTACAAG
    AGCTCCTGCA GCATCGACGT CACCTTCTTC CCCTTCGACC AGCAGAACTG CAAGATGAAG
    TTTGGCTCCT GGACTTATGA CAAGGCCAAG ATCGACCTGG AGCAGATGGA GCAGACTGTG
    GACCTGAAGG ACTACTGGGA GAGCGGCGAG TGGGCCATCG TCAATGCCAC GGGCACCTAC
    AACAGCAAGA AGTACGACTG CTGCGCCGAG ATCTACCCCG ACGTCACCTA CGCCTTCGTC
    ATCCGGCGGC TGCCGCTCTT CTACACCATC AACCTCATCA TCCCCTGCCT GCTCATCTCC
    TGCCTCACTG TGCTGGTCTT CTACCTGCCC TCCGACTGCG GCGAGAAGAT CACGCTGTGC
    ATTTCGGTGC TGCTGTCACT CACCGTCTTC CTGCTGCTCA TCACTGAGAT CATCCCGTCC
    ACCTCGCTGG TCATCCCGCT CATCGGCGAG TACCTGCTGT TCACCATGAT CTTCGTCACC
    CTGTCCATCG TCATCACCGT CTTCGTGCTC AATGTGCACC ACCGCTCCCC CAGCACCCAC
    ACCATGCCCC ACTGGGTGCG GGGGGCCCTT CTGGGCTGTG TGCCCCGGTG GCTTCTGATG
    AACCGGCCCC CACCACCCGT GGAGCTCTGC CACCCCCTAC GCCTGAAGCT CAGCCCCTCT
    TATCACTGGC TGGAGAGCAA CGTGGATGCC GAGGAGAGGG AGGTGGTGGT GGAGGAGGAG
    GACAGATGGG CATGTGCAGG TCATGTGGCC CCCTCTGTGG GCACCCTCTG CAGCCACGGC
    CACCTGCACT CTGGGGCCTC AGGTCCCAAG GCTGAGGCTC TGCTGCAGGA GGGTGAGCTG
    CTGCTATCAC CCCACATGCA GAAGGCACTG GAAGGTGTGC ACTACATTGC CGACCACCTG
    CGGTCTGAGG ATGCTGACTC TTCGGTGAAG GAGGACTGGA AGTATGTTGC CATGGTCATC
    GACAGGATCT TCCTCTGGCT GTTTATCATC GTCTGCTTCC TGGGGACCAT CGGCCTCTTT
    CTGCCTCCGT TCCTAGCTGG AATGATCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu17978
    Clone ID Related Accession (Same CDS sequence) NM_000742.3 , XM_006716282.1 , NM_000742.4 , XM_011544388.1
    Accession Version XM_006716282.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1590bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-05
    Organism Homo sapiens(human)
    Product neuronal acetylcholine receptor subunit alpha-2 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000008.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    ATGGGCCCCT CCTGTCCTGT GTTCCTGTCC TTCACAAAGC TCAGCCTGTG GTGGCTCCTT 
    CTGACCCCAG CAGGTGGAGA GGAAGCTAAG CGCCCACCTC CCAGGGCTCC TGGAGACCCA
    CTCTCCTCTC CCAGTCCCAC GGCATTGCCG CAGGGAGGCT CGCATACCGA GACTGAGGAC
    CGGCTCTTCA AACACCTCTT CCGGGGCTAC AACCGCTGGG CGCGCCCGGT GCCCAACACT
    TCAGACGTGG TGATTGTGCG CTTTGGACTG TCCATCGCTC AGCTCATCGA TGTGGATGAG
    AAGAACCAAA TGATGACCAC CAACGTCTGG CTAAAACAGG AGTGGAGCGA CTACAAACTG
    CGCTGGAACC CCACTGATTT TGGCAACATC ACATCTCTCA GGGTCCCTTC TGAGATGATC
    TGGATCCCCG ACATTGTTCT CTACAACAAT GCAGATGGGG AGTTTGCAGT GACCCACATG
    ACCAAGGCCC ACCTCTTCTC CACGGGCACT GTGCACTGGG TGCCCCCGGC CATCTACAAG
    AGCTCCTGCA GCATCGACGT CACCTTCTTC CCCTTCGACC AGCAGAACTG CAAGATGAAG
    TTTGGCTCCT GGACTTATGA CAAGGCCAAG ATCGACCTGG AGCAGATGGA GCAGACTGTG
    GACCTGAAGG ACTACTGGGA GAGCGGCGAG TGGGCCATCG TCAATGCCAC GGGCACCTAC
    AACAGCAAGA AGTACGACTG CTGCGCCGAG ATCTACCCCG ACGTCACCTA CGCCTTCGTC
    ATCCGGCGGC TGCCGCTCTT CTACACCATC AACCTCATCA TCCCCTGCCT GCTCATCTCC
    TGCCTCACTG TGCTGGTCTT CTACCTGCCC TCCGACTGCG GCGAGAAGAT CACGCTGTGC
    ATTTCGGTGC TGCTGTCACT CACCGTCTTC CTGCTGCTCA TCACTGAGAT CATCCCGTCC
    ACCTCGCTGG TCATCCCGCT CATCGGCGAG TACCTGCTGT TCACCATGAT CTTCGTCACC
    CTGTCCATCG TCATCACCGT CTTCGTGCTC AATGTGCACC ACCGCTCCCC CAGCACCCAC
    ACCATGCCCC ACTGGGTGCG GGGGGCCCTT CTGGGCTGTG TGCCCCGGTG GCTTCTGATG
    AACCGGCCCC CACCACCCGT GGAGCTCTGC CACCCCCTAC GCCTGAAGCT CAGCCCCTCT
    TATCACTGGC TGGAGAGCAA CGTGGATGCC GAGGAGAGGG AGGTGGTGGT GGAGGAGGAG
    GACAGATGGG CATGTGCAGG TCATGTGGCC CCCTCTGTGG GCACCCTCTG CAGCCACGGC
    CACCTGCACT CTGGGGCCTC AGGTCCCAAG GCTGAGGCTC TGCTGCAGGA GGGTGAGCTG
    CTGCTATCAC CCCACATGCA GAAGGCACTG GAAGGTGTGC ACTACATTGC CGACCACCTG
    CGGTCTGAGG ATGCTGACTC TTCGGTGAAG GAGGACTGGA AGTATGTTGC CATGGTCATC
    GACAGGATCT TCCTCTGGCT GTTTATCATC GTCTGCTTCC TGGGGACCAT CGGCCTCTTT
    CTGCCTCCGT TCCTAGCTGG AATGATCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006716345.1
    CDS521..2110
    Translation

    Target ORF information:

    RefSeq Version XM_006716282.1
    Organism Homo sapiens(human)
    Definition Homo sapiens cholinergic receptor nicotinic alpha 2 subunit (CHRNA2), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006716282.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    ATGGGCCCCT CCTGTCCTGT GTTCCTGTCC TTCACAAAGC TCAGCCTGTG GTGGCTCCTT 
    CTGACCCCAG CAGGTGGAGA GGAAGCTAAG CGCCCACCTC CCAGGGCTCC TGGAGACCCA
    CTCTCCTCTC CCAGTCCCAC GGCATTGCCG CAGGGAGGCT CGCATACCGA GACTGAGGAC
    CGGCTCTTCA AACACCTCTT CCGGGGCTAC AACCGCTGGG CGCGCCCGGT GCCCAACACT
    TCAGACGTGG TGATTGTGCG CTTTGGACTG TCCATCGCTC AGCTCATCGA TGTGGATGAG
    AAGAACCAAA TGATGACCAC CAACGTCTGG CTAAAACAGG AGTGGAGCGA CTACAAACTG
    CGCTGGAACC CCACTGATTT TGGCAACATC ACATCTCTCA GGGTCCCTTC TGAGATGATC
    TGGATCCCCG ACATTGTTCT CTACAACAAT GCAGATGGGG AGTTTGCAGT GACCCACATG
    ACCAAGGCCC ACCTCTTCTC CACGGGCACT GTGCACTGGG TGCCCCCGGC CATCTACAAG
    AGCTCCTGCA GCATCGACGT CACCTTCTTC CCCTTCGACC AGCAGAACTG CAAGATGAAG
    TTTGGCTCCT GGACTTATGA CAAGGCCAAG ATCGACCTGG AGCAGATGGA GCAGACTGTG
    GACCTGAAGG ACTACTGGGA GAGCGGCGAG TGGGCCATCG TCAATGCCAC GGGCACCTAC
    AACAGCAAGA AGTACGACTG CTGCGCCGAG ATCTACCCCG ACGTCACCTA CGCCTTCGTC
    ATCCGGCGGC TGCCGCTCTT CTACACCATC AACCTCATCA TCCCCTGCCT GCTCATCTCC
    TGCCTCACTG TGCTGGTCTT CTACCTGCCC TCCGACTGCG GCGAGAAGAT CACGCTGTGC
    ATTTCGGTGC TGCTGTCACT CACCGTCTTC CTGCTGCTCA TCACTGAGAT CATCCCGTCC
    ACCTCGCTGG TCATCCCGCT CATCGGCGAG TACCTGCTGT TCACCATGAT CTTCGTCACC
    CTGTCCATCG TCATCACCGT CTTCGTGCTC AATGTGCACC ACCGCTCCCC CAGCACCCAC
    ACCATGCCCC ACTGGGTGCG GGGGGCCCTT CTGGGCTGTG TGCCCCGGTG GCTTCTGATG
    AACCGGCCCC CACCACCCGT GGAGCTCTGC CACCCCCTAC GCCTGAAGCT CAGCCCCTCT
    TATCACTGGC TGGAGAGCAA CGTGGATGCC GAGGAGAGGG AGGTGGTGGT GGAGGAGGAG
    GACAGATGGG CATGTGCAGG TCATGTGGCC CCCTCTGTGG GCACCCTCTG CAGCCACGGC
    CACCTGCACT CTGGGGCCTC AGGTCCCAAG GCTGAGGCTC TGCTGCAGGA GGGTGAGCTG
    CTGCTATCAC CCCACATGCA GAAGGCACTG GAAGGTGTGC ACTACATTGC CGACCACCTG
    CGGTCTGAGG ATGCTGACTC TTCGGTGAAG GAGGACTGGA AGTATGTTGC CATGGTCATC
    GACAGGATCT TCCTCTGGCT GTTTATCATC GTCTGCTTCC TGGGGACCAT CGGCCTCTTT
    CTGCCTCCGT TCCTAGCTGG AATGATCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu17978
    Clone ID Related Accession (Same CDS sequence) NM_000742.3 , XM_006716282.1 , NM_000742.4 , XM_011544388.1
    Accession Version XM_011544388.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1590bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-05
    Organism Homo sapiens(human)
    Product neuronal acetylcholine receptor subunit alpha-2 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000008.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    ATGGGCCCCT CCTGTCCTGT GTTCCTGTCC TTCACAAAGC TCAGCCTGTG GTGGCTCCTT 
    CTGACCCCAG CAGGTGGAGA GGAAGCTAAG CGCCCACCTC CCAGGGCTCC TGGAGACCCA
    CTCTCCTCTC CCAGTCCCAC GGCATTGCCG CAGGGAGGCT CGCATACCGA GACTGAGGAC
    CGGCTCTTCA AACACCTCTT CCGGGGCTAC AACCGCTGGG CGCGCCCGGT GCCCAACACT
    TCAGACGTGG TGATTGTGCG CTTTGGACTG TCCATCGCTC AGCTCATCGA TGTGGATGAG
    AAGAACCAAA TGATGACCAC CAACGTCTGG CTAAAACAGG AGTGGAGCGA CTACAAACTG
    CGCTGGAACC CCACTGATTT TGGCAACATC ACATCTCTCA GGGTCCCTTC TGAGATGATC
    TGGATCCCCG ACATTGTTCT CTACAACAAT GCAGATGGGG AGTTTGCAGT GACCCACATG
    ACCAAGGCCC ACCTCTTCTC CACGGGCACT GTGCACTGGG TGCCCCCGGC CATCTACAAG
    AGCTCCTGCA GCATCGACGT CACCTTCTTC CCCTTCGACC AGCAGAACTG CAAGATGAAG
    TTTGGCTCCT GGACTTATGA CAAGGCCAAG ATCGACCTGG AGCAGATGGA GCAGACTGTG
    GACCTGAAGG ACTACTGGGA GAGCGGCGAG TGGGCCATCG TCAATGCCAC GGGCACCTAC
    AACAGCAAGA AGTACGACTG CTGCGCCGAG ATCTACCCCG ACGTCACCTA CGCCTTCGTC
    ATCCGGCGGC TGCCGCTCTT CTACACCATC AACCTCATCA TCCCCTGCCT GCTCATCTCC
    TGCCTCACTG TGCTGGTCTT CTACCTGCCC TCCGACTGCG GCGAGAAGAT CACGCTGTGC
    ATTTCGGTGC TGCTGTCACT CACCGTCTTC CTGCTGCTCA TCACTGAGAT CATCCCGTCC
    ACCTCGCTGG TCATCCCGCT CATCGGCGAG TACCTGCTGT TCACCATGAT CTTCGTCACC
    CTGTCCATCG TCATCACCGT CTTCGTGCTC AATGTGCACC ACCGCTCCCC CAGCACCCAC
    ACCATGCCCC ACTGGGTGCG GGGGGCCCTT CTGGGCTGTG TGCCCCGGTG GCTTCTGATG
    AACCGGCCCC CACCACCCGT GGAGCTCTGC CACCCCCTAC GCCTGAAGCT CAGCCCCTCT
    TATCACTGGC TGGAGAGCAA CGTGGATGCC GAGGAGAGGG AGGTGGTGGT GGAGGAGGAG
    GACAGATGGG CATGTGCAGG TCATGTGGCC CCCTCTGTGG GCACCCTCTG CAGCCACGGC
    CACCTGCACT CTGGGGCCTC AGGTCCCAAG GCTGAGGCTC TGCTGCAGGA GGGTGAGCTG
    CTGCTATCAC CCCACATGCA GAAGGCACTG GAAGGTGTGC ACTACATTGC CGACCACCTG
    CGGTCTGAGG ATGCTGACTC TTCGGTGAAG GAGGACTGGA AGTATGTTGC CATGGTCATC
    GACAGGATCT TCCTCTGGCT GTTTATCATC GTCTGCTTCC TGGGGACCAT CGGCCTCTTT
    CTGCCTCCGT TCCTAGCTGG AATGATCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_011542690.1
    CDS612..2201
    Translation

    Target ORF information:

    RefSeq Version XM_011544388.1
    Organism Homo sapiens(human)
    Definition Homo sapiens cholinergic receptor nicotinic alpha 2 subunit (CHRNA2), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_011544388.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    ATGGGCCCCT CCTGTCCTGT GTTCCTGTCC TTCACAAAGC TCAGCCTGTG GTGGCTCCTT 
    CTGACCCCAG CAGGTGGAGA GGAAGCTAAG CGCCCACCTC CCAGGGCTCC TGGAGACCCA
    CTCTCCTCTC CCAGTCCCAC GGCATTGCCG CAGGGAGGCT CGCATACCGA GACTGAGGAC
    CGGCTCTTCA AACACCTCTT CCGGGGCTAC AACCGCTGGG CGCGCCCGGT GCCCAACACT
    TCAGACGTGG TGATTGTGCG CTTTGGACTG TCCATCGCTC AGCTCATCGA TGTGGATGAG
    AAGAACCAAA TGATGACCAC CAACGTCTGG CTAAAACAGG AGTGGAGCGA CTACAAACTG
    CGCTGGAACC CCACTGATTT TGGCAACATC ACATCTCTCA GGGTCCCTTC TGAGATGATC
    TGGATCCCCG ACATTGTTCT CTACAACAAT GCAGATGGGG AGTTTGCAGT GACCCACATG
    ACCAAGGCCC ACCTCTTCTC CACGGGCACT GTGCACTGGG TGCCCCCGGC CATCTACAAG
    AGCTCCTGCA GCATCGACGT CACCTTCTTC CCCTTCGACC AGCAGAACTG CAAGATGAAG
    TTTGGCTCCT GGACTTATGA CAAGGCCAAG ATCGACCTGG AGCAGATGGA GCAGACTGTG
    GACCTGAAGG ACTACTGGGA GAGCGGCGAG TGGGCCATCG TCAATGCCAC GGGCACCTAC
    AACAGCAAGA AGTACGACTG CTGCGCCGAG ATCTACCCCG ACGTCACCTA CGCCTTCGTC
    ATCCGGCGGC TGCCGCTCTT CTACACCATC AACCTCATCA TCCCCTGCCT GCTCATCTCC
    TGCCTCACTG TGCTGGTCTT CTACCTGCCC TCCGACTGCG GCGAGAAGAT CACGCTGTGC
    ATTTCGGTGC TGCTGTCACT CACCGTCTTC CTGCTGCTCA TCACTGAGAT CATCCCGTCC
    ACCTCGCTGG TCATCCCGCT CATCGGCGAG TACCTGCTGT TCACCATGAT CTTCGTCACC
    CTGTCCATCG TCATCACCGT CTTCGTGCTC AATGTGCACC ACCGCTCCCC CAGCACCCAC
    ACCATGCCCC ACTGGGTGCG GGGGGCCCTT CTGGGCTGTG TGCCCCGGTG GCTTCTGATG
    AACCGGCCCC CACCACCCGT GGAGCTCTGC CACCCCCTAC GCCTGAAGCT CAGCCCCTCT
    TATCACTGGC TGGAGAGCAA CGTGGATGCC GAGGAGAGGG AGGTGGTGGT GGAGGAGGAG
    GACAGATGGG CATGTGCAGG TCATGTGGCC CCCTCTGTGG GCACCCTCTG CAGCCACGGC
    CACCTGCACT CTGGGGCCTC AGGTCCCAAG GCTGAGGCTC TGCTGCAGGA GGGTGAGCTG
    CTGCTATCAC CCCACATGCA GAAGGCACTG GAAGGTGTGC ACTACATTGC CGACCACCTG
    CGGTCTGAGG ATGCTGACTC TTCGGTGAAG GAGGACTGGA AGTATGTTGC CATGGTCATC
    GACAGGATCT TCCTCTGGCT GTTTATCATC GTCTGCTTCC TGGGGACCAT CGGCCTCTTT
    CTGCCTCCGT TCCTAGCTGG AATGATCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu41986
    Clone ID Related Accession (Same CDS sequence) NM_001347706.2 , NM_001347706.1 , NM_001347705.2 , NM_001347705.1
    Accession Version NM_001347706.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1113bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-10-25
    Organism Homo sapiens(human)
    Product neuronal acetylcholine receptor subunit alpha-2 isoform 3
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AF311103.5. On May 31, 2019 this sequence version replaced NM_001347706.1. Transcript Variant: This variant (4), as well as variant 3, encodes isoform 3. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660805.13866.1, SRR1803617.143390.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2145743, SAMEA2153427 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    ATGACCAAGG CCCACCTCTT CTCCACGGGC ACTGTGCACT GGGTGCCCCC GGCCATCTAC 
    AAGAGCTCCT GCAGCATCGA CGTCACCTTC TTCCCCTTCG ACCAGCAGAA CTGCAAGATG
    AAGTTTGGCT CCTGGACTTA TGACAAGGCC AAGATCGACC TGGAGCAGAT GGAGCAGACT
    GTGGACCTGA AGGACTACTG GGAGAGCGGC GAGTGGGCCA TCGTCAATGC CACGGGCACC
    TACAACAGCA AGAAGTACGA CTGCTGCGCC GAGATCTACC CCGACGTCAC CTACGCCTTC
    GTCATCCGGC GGCTGCCGCT CTTCTACACC ATCAACCTCA TCATCCCCTG CCTGCTCATC
    TCCTGCCTCA CTGTGCTGGT CTTCTACCTG CCCTCCGACT GCGGCGAGAA GATCACGCTG
    TGCATTTCGG TGCTGCTGTC ACTCACCGTC TTCCTGCTGC TCATCACTGA GATCATCCCG
    TCCACCTCGC TGGTCATCCC GCTCATCGGC GAGTACCTGC TGTTCACCAT GATCTTCGTC
    ACCCTGTCCA TCGTCATCAC CGTCTTCGTG CTCAATGTGC ACCACCGCTC CCCCAGCACC
    CACACCATGC CCCACTGGGT GCGGGGGGCC CTTCTGGGCT GTGTGCCCCG GTGGCTTCTG
    ATGAACCGGC CCCCACCACC CGTGGAGCTC TGCCACCCCC TACGCCTGAA GCTCAGCCCC
    TCTTATCACT GGCTGGAGAG CAACGTGGAT GCCGAGGAGA GGGAGGTGGT GGTGGAGGAG
    GAGGACAGAT GGGCATGTGC AGGTCATGTG GCCCCCTCTG TGGGCACCCT CTGCAGCCAC
    GGCCACCTGC ACTCTGGGGC CTCAGGTCCC AAGGCTGAGG CTCTGCTGCA GGAGGGTGAG
    CTGCTGCTAT CACCCCACAT GCAGAAGGCA CTGGAAGGTG TGCACTACAT TGCCGACCAC
    CTGCGGTCTG AGGATGCTGA CTCTTCGGTG AAGGAGGACT GGAAGTATGT TGCCATGGTC
    ATCGACAGGA TCTTCCTCTG GCTGTTTATC ATCGTCTGCT TCCTGGGGAC CATCGGCCTC
    TTTCTGCCTC CGTTCCTAGC TGGAATGATC TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001334635.1
    CDS1047..2159
    Translation

    Target ORF information:

    RefSeq Version NM_001347706.2
    Organism Homo sapiens(human)
    Definition Homo sapiens cholinergic receptor nicotinic alpha 2 subunit (CHRNA2), transcript variant 4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001347706.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    ATGACCAAGG CCCACCTCTT CTCCACGGGC ACTGTGCACT GGGTGCCCCC GGCCATCTAC 
    AAGAGCTCCT GCAGCATCGA CGTCACCTTC TTCCCCTTCG ACCAGCAGAA CTGCAAGATG
    AAGTTTGGCT CCTGGACTTA TGACAAGGCC AAGATCGACC TGGAGCAGAT GGAGCAGACT
    GTGGACCTGA AGGACTACTG GGAGAGCGGC GAGTGGGCCA TCGTCAATGC CACGGGCACC
    TACAACAGCA AGAAGTACGA CTGCTGCGCC GAGATCTACC CCGACGTCAC CTACGCCTTC
    GTCATCCGGC GGCTGCCGCT CTTCTACACC ATCAACCTCA TCATCCCCTG CCTGCTCATC
    TCCTGCCTCA CTGTGCTGGT CTTCTACCTG CCCTCCGACT GCGGCGAGAA GATCACGCTG
    TGCATTTCGG TGCTGCTGTC ACTCACCGTC TTCCTGCTGC TCATCACTGA GATCATCCCG
    TCCACCTCGC TGGTCATCCC GCTCATCGGC GAGTACCTGC TGTTCACCAT GATCTTCGTC
    ACCCTGTCCA TCGTCATCAC CGTCTTCGTG CTCAATGTGC ACCACCGCTC CCCCAGCACC
    CACACCATGC CCCACTGGGT GCGGGGGGCC CTTCTGGGCT GTGTGCCCCG GTGGCTTCTG
    ATGAACCGGC CCCCACCACC CGTGGAGCTC TGCCACCCCC TACGCCTGAA GCTCAGCCCC
    TCTTATCACT GGCTGGAGAG CAACGTGGAT GCCGAGGAGA GGGAGGTGGT GGTGGAGGAG
    GAGGACAGAT GGGCATGTGC AGGTCATGTG GCCCCCTCTG TGGGCACCCT CTGCAGCCAC
    GGCCACCTGC ACTCTGGGGC CTCAGGTCCC AAGGCTGAGG CTCTGCTGCA GGAGGGTGAG
    CTGCTGCTAT CACCCCACAT GCAGAAGGCA CTGGAAGGTG TGCACTACAT TGCCGACCAC
    CTGCGGTCTG AGGATGCTGA CTCTTCGGTG AAGGAGGACT GGAAGTATGT TGCCATGGTC
    ATCGACAGGA TCTTCCTCTG GCTGTTTATC ATCGTCTGCT TCCTGGGGAC CATCGGCCTC
    TTTCTGCCTC CGTTCCTAGC TGGAATGATC TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu41986
    Clone ID Related Accession (Same CDS sequence) NM_001347706.2 , NM_001347706.1 , NM_001347705.2 , NM_001347705.1
    Accession Version NM_001347706.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1113bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-02-24
    Organism Homo sapiens(human)
    Product neuronal acetylcholine receptor subunit alpha-2 isoform 3
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AF311103.5. On Dec 9, 2016 this sequence version replaced XM_005273397.2. Transcript Variant: This variant (4), as well as variant 3, encodes isoform 3. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660805.13866.1, SRR1803617.143390.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2145743, SAMEA2153427 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    ATGACCAAGG CCCACCTCTT CTCCACGGGC ACTGTGCACT GGGTGCCCCC GGCCATCTAC 
    AAGAGCTCCT GCAGCATCGA CGTCACCTTC TTCCCCTTCG ACCAGCAGAA CTGCAAGATG
    AAGTTTGGCT CCTGGACTTA TGACAAGGCC AAGATCGACC TGGAGCAGAT GGAGCAGACT
    GTGGACCTGA AGGACTACTG GGAGAGCGGC GAGTGGGCCA TCGTCAATGC CACGGGCACC
    TACAACAGCA AGAAGTACGA CTGCTGCGCC GAGATCTACC CCGACGTCAC CTACGCCTTC
    GTCATCCGGC GGCTGCCGCT CTTCTACACC ATCAACCTCA TCATCCCCTG CCTGCTCATC
    TCCTGCCTCA CTGTGCTGGT CTTCTACCTG CCCTCCGACT GCGGCGAGAA GATCACGCTG
    TGCATTTCGG TGCTGCTGTC ACTCACCGTC TTCCTGCTGC TCATCACTGA GATCATCCCG
    TCCACCTCGC TGGTCATCCC GCTCATCGGC GAGTACCTGC TGTTCACCAT GATCTTCGTC
    ACCCTGTCCA TCGTCATCAC CGTCTTCGTG CTCAATGTGC ACCACCGCTC CCCCAGCACC
    CACACCATGC CCCACTGGGT GCGGGGGGCC CTTCTGGGCT GTGTGCCCCG GTGGCTTCTG
    ATGAACCGGC CCCCACCACC CGTGGAGCTC TGCCACCCCC TACGCCTGAA GCTCAGCCCC
    TCTTATCACT GGCTGGAGAG CAACGTGGAT GCCGAGGAGA GGGAGGTGGT GGTGGAGGAG
    GAGGACAGAT GGGCATGTGC AGGTCATGTG GCCCCCTCTG TGGGCACCCT CTGCAGCCAC
    GGCCACCTGC ACTCTGGGGC CTCAGGTCCC AAGGCTGAGG CTCTGCTGCA GGAGGGTGAG
    CTGCTGCTAT CACCCCACAT GCAGAAGGCA CTGGAAGGTG TGCACTACAT TGCCGACCAC
    CTGCGGTCTG AGGATGCTGA CTCTTCGGTG AAGGAGGACT GGAAGTATGT TGCCATGGTC
    ATCGACAGGA TCTTCCTCTG GCTGTTTATC ATCGTCTGCT TCCTGGGGAC CATCGGCCTC
    TTTCTGCCTC CGTTCCTAGC TGGAATGATC TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001334635.1
    CDS1082..2194
    Translation

    Target ORF information:

    RefSeq Version NM_001347706.1
    Organism Homo sapiens(human)
    Definition Homo sapiens cholinergic receptor nicotinic alpha 2 subunit (CHRNA2), transcript variant 4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001347706.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    ATGACCAAGG CCCACCTCTT CTCCACGGGC ACTGTGCACT GGGTGCCCCC GGCCATCTAC 
    AAGAGCTCCT GCAGCATCGA CGTCACCTTC TTCCCCTTCG ACCAGCAGAA CTGCAAGATG
    AAGTTTGGCT CCTGGACTTA TGACAAGGCC AAGATCGACC TGGAGCAGAT GGAGCAGACT
    GTGGACCTGA AGGACTACTG GGAGAGCGGC GAGTGGGCCA TCGTCAATGC CACGGGCACC
    TACAACAGCA AGAAGTACGA CTGCTGCGCC GAGATCTACC CCGACGTCAC CTACGCCTTC
    GTCATCCGGC GGCTGCCGCT CTTCTACACC ATCAACCTCA TCATCCCCTG CCTGCTCATC
    TCCTGCCTCA CTGTGCTGGT CTTCTACCTG CCCTCCGACT GCGGCGAGAA GATCACGCTG
    TGCATTTCGG TGCTGCTGTC ACTCACCGTC TTCCTGCTGC TCATCACTGA GATCATCCCG
    TCCACCTCGC TGGTCATCCC GCTCATCGGC GAGTACCTGC TGTTCACCAT GATCTTCGTC
    ACCCTGTCCA TCGTCATCAC CGTCTTCGTG CTCAATGTGC ACCACCGCTC CCCCAGCACC
    CACACCATGC CCCACTGGGT GCGGGGGGCC CTTCTGGGCT GTGTGCCCCG GTGGCTTCTG
    ATGAACCGGC CCCCACCACC CGTGGAGCTC TGCCACCCCC TACGCCTGAA GCTCAGCCCC
    TCTTATCACT GGCTGGAGAG CAACGTGGAT GCCGAGGAGA GGGAGGTGGT GGTGGAGGAG
    GAGGACAGAT GGGCATGTGC AGGTCATGTG GCCCCCTCTG TGGGCACCCT CTGCAGCCAC
    GGCCACCTGC ACTCTGGGGC CTCAGGTCCC AAGGCTGAGG CTCTGCTGCA GGAGGGTGAG
    CTGCTGCTAT CACCCCACAT GCAGAAGGCA CTGGAAGGTG TGCACTACAT TGCCGACCAC
    CTGCGGTCTG AGGATGCTGA CTCTTCGGTG AAGGAGGACT GGAAGTATGT TGCCATGGTC
    ATCGACAGGA TCTTCCTCTG GCTGTTTATC ATCGTCTGCT TCCTGGGGAC CATCGGCCTC
    TTTCTGCCTC CGTTCCTAGC TGGAATGATC TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu41986
    Clone ID Related Accession (Same CDS sequence) NM_001347706.2 , NM_001347706.1 , NM_001347705.2 , NM_001347705.1
    Accession Version NM_001347705.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1113bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-10-25
    Organism Homo sapiens(human)
    Product neuronal acetylcholine receptor subunit alpha-2 isoform 3
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AF311103.5. On May 31, 2019 this sequence version replaced NM_001347705.1. Transcript Variant: This variant (3), as well as variant 4, encodes isoform 3. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803612.194490.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2153427, SAMEA2155984 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    ATGACCAAGG CCCACCTCTT CTCCACGGGC ACTGTGCACT GGGTGCCCCC GGCCATCTAC 
    AAGAGCTCCT GCAGCATCGA CGTCACCTTC TTCCCCTTCG ACCAGCAGAA CTGCAAGATG
    AAGTTTGGCT CCTGGACTTA TGACAAGGCC AAGATCGACC TGGAGCAGAT GGAGCAGACT
    GTGGACCTGA AGGACTACTG GGAGAGCGGC GAGTGGGCCA TCGTCAATGC CACGGGCACC
    TACAACAGCA AGAAGTACGA CTGCTGCGCC GAGATCTACC CCGACGTCAC CTACGCCTTC
    GTCATCCGGC GGCTGCCGCT CTTCTACACC ATCAACCTCA TCATCCCCTG CCTGCTCATC
    TCCTGCCTCA CTGTGCTGGT CTTCTACCTG CCCTCCGACT GCGGCGAGAA GATCACGCTG
    TGCATTTCGG TGCTGCTGTC ACTCACCGTC TTCCTGCTGC TCATCACTGA GATCATCCCG
    TCCACCTCGC TGGTCATCCC GCTCATCGGC GAGTACCTGC TGTTCACCAT GATCTTCGTC
    ACCCTGTCCA TCGTCATCAC CGTCTTCGTG CTCAATGTGC ACCACCGCTC CCCCAGCACC
    CACACCATGC CCCACTGGGT GCGGGGGGCC CTTCTGGGCT GTGTGCCCCG GTGGCTTCTG
    ATGAACCGGC CCCCACCACC CGTGGAGCTC TGCCACCCCC TACGCCTGAA GCTCAGCCCC
    TCTTATCACT GGCTGGAGAG CAACGTGGAT GCCGAGGAGA GGGAGGTGGT GGTGGAGGAG
    GAGGACAGAT GGGCATGTGC AGGTCATGTG GCCCCCTCTG TGGGCACCCT CTGCAGCCAC
    GGCCACCTGC ACTCTGGGGC CTCAGGTCCC AAGGCTGAGG CTCTGCTGCA GGAGGGTGAG
    CTGCTGCTAT CACCCCACAT GCAGAAGGCA CTGGAAGGTG TGCACTACAT TGCCGACCAC
    CTGCGGTCTG AGGATGCTGA CTCTTCGGTG AAGGAGGACT GGAAGTATGT TGCCATGGTC
    ATCGACAGGA TCTTCCTCTG GCTGTTTATC ATCGTCTGCT TCCTGGGGAC CATCGGCCTC
    TTTCTGCCTC CGTTCCTAGC TGGAATGATC TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001334634.1
    CDS1002..2114
    Translation

    Target ORF information:

    RefSeq Version NM_001347705.2
    Organism Homo sapiens(human)
    Definition Homo sapiens cholinergic receptor nicotinic alpha 2 subunit (CHRNA2), transcript variant 3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001347705.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    ATGACCAAGG CCCACCTCTT CTCCACGGGC ACTGTGCACT GGGTGCCCCC GGCCATCTAC 
    AAGAGCTCCT GCAGCATCGA CGTCACCTTC TTCCCCTTCG ACCAGCAGAA CTGCAAGATG
    AAGTTTGGCT CCTGGACTTA TGACAAGGCC AAGATCGACC TGGAGCAGAT GGAGCAGACT
    GTGGACCTGA AGGACTACTG GGAGAGCGGC GAGTGGGCCA TCGTCAATGC CACGGGCACC
    TACAACAGCA AGAAGTACGA CTGCTGCGCC GAGATCTACC CCGACGTCAC CTACGCCTTC
    GTCATCCGGC GGCTGCCGCT CTTCTACACC ATCAACCTCA TCATCCCCTG CCTGCTCATC
    TCCTGCCTCA CTGTGCTGGT CTTCTACCTG CCCTCCGACT GCGGCGAGAA GATCACGCTG
    TGCATTTCGG TGCTGCTGTC ACTCACCGTC TTCCTGCTGC TCATCACTGA GATCATCCCG
    TCCACCTCGC TGGTCATCCC GCTCATCGGC GAGTACCTGC TGTTCACCAT GATCTTCGTC
    ACCCTGTCCA TCGTCATCAC CGTCTTCGTG CTCAATGTGC ACCACCGCTC CCCCAGCACC
    CACACCATGC CCCACTGGGT GCGGGGGGCC CTTCTGGGCT GTGTGCCCCG GTGGCTTCTG
    ATGAACCGGC CCCCACCACC CGTGGAGCTC TGCCACCCCC TACGCCTGAA GCTCAGCCCC
    TCTTATCACT GGCTGGAGAG CAACGTGGAT GCCGAGGAGA GGGAGGTGGT GGTGGAGGAG
    GAGGACAGAT GGGCATGTGC AGGTCATGTG GCCCCCTCTG TGGGCACCCT CTGCAGCCAC
    GGCCACCTGC ACTCTGGGGC CTCAGGTCCC AAGGCTGAGG CTCTGCTGCA GGAGGGTGAG
    CTGCTGCTAT CACCCCACAT GCAGAAGGCA CTGGAAGGTG TGCACTACAT TGCCGACCAC
    CTGCGGTCTG AGGATGCTGA CTCTTCGGTG AAGGAGGACT GGAAGTATGT TGCCATGGTC
    ATCGACAGGA TCTTCCTCTG GCTGTTTATC ATCGTCTGCT TCCTGGGGAC CATCGGCCTC
    TTTCTGCCTC CGTTCCTAGC TGGAATGATC TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu41986
    Clone ID Related Accession (Same CDS sequence) NM_001347706.2 , NM_001347706.1 , NM_001347705.2 , NM_001347705.1
    Accession Version NM_001347705.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1113bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-02-25
    Organism Homo sapiens(human)
    Product neuronal acetylcholine receptor subunit alpha-2 isoform 3
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AF311103.5. On Dec 9, 2016 this sequence version replaced XM_017013003.1. Transcript Variant: This variant (3), as well as variant 4, encodes isoform 3. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803612.194490.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2153427, SAMEA2155984 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    ATGACCAAGG CCCACCTCTT CTCCACGGGC ACTGTGCACT GGGTGCCCCC GGCCATCTAC 
    AAGAGCTCCT GCAGCATCGA CGTCACCTTC TTCCCCTTCG ACCAGCAGAA CTGCAAGATG
    AAGTTTGGCT CCTGGACTTA TGACAAGGCC AAGATCGACC TGGAGCAGAT GGAGCAGACT
    GTGGACCTGA AGGACTACTG GGAGAGCGGC GAGTGGGCCA TCGTCAATGC CACGGGCACC
    TACAACAGCA AGAAGTACGA CTGCTGCGCC GAGATCTACC CCGACGTCAC CTACGCCTTC
    GTCATCCGGC GGCTGCCGCT CTTCTACACC ATCAACCTCA TCATCCCCTG CCTGCTCATC
    TCCTGCCTCA CTGTGCTGGT CTTCTACCTG CCCTCCGACT GCGGCGAGAA GATCACGCTG
    TGCATTTCGG TGCTGCTGTC ACTCACCGTC TTCCTGCTGC TCATCACTGA GATCATCCCG
    TCCACCTCGC TGGTCATCCC GCTCATCGGC GAGTACCTGC TGTTCACCAT GATCTTCGTC
    ACCCTGTCCA TCGTCATCAC CGTCTTCGTG CTCAATGTGC ACCACCGCTC CCCCAGCACC
    CACACCATGC CCCACTGGGT GCGGGGGGCC CTTCTGGGCT GTGTGCCCCG GTGGCTTCTG
    ATGAACCGGC CCCCACCACC CGTGGAGCTC TGCCACCCCC TACGCCTGAA GCTCAGCCCC
    TCTTATCACT GGCTGGAGAG CAACGTGGAT GCCGAGGAGA GGGAGGTGGT GGTGGAGGAG
    GAGGACAGAT GGGCATGTGC AGGTCATGTG GCCCCCTCTG TGGGCACCCT CTGCAGCCAC
    GGCCACCTGC ACTCTGGGGC CTCAGGTCCC AAGGCTGAGG CTCTGCTGCA GGAGGGTGAG
    CTGCTGCTAT CACCCCACAT GCAGAAGGCA CTGGAAGGTG TGCACTACAT TGCCGACCAC
    CTGCGGTCTG AGGATGCTGA CTCTTCGGTG AAGGAGGACT GGAAGTATGT TGCCATGGTC
    ATCGACAGGA TCTTCCTCTG GCTGTTTATC ATCGTCTGCT TCCTGGGGAC CATCGGCCTC
    TTTCTGCCTC CGTTCCTAGC TGGAATGATC TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001334634.1
    CDS1037..2149
    Translation

    Target ORF information:

    RefSeq Version NM_001347705.1
    Organism Homo sapiens(human)
    Definition Homo sapiens cholinergic receptor nicotinic alpha 2 subunit (CHRNA2), transcript variant 3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001347705.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    ATGACCAAGG CCCACCTCTT CTCCACGGGC ACTGTGCACT GGGTGCCCCC GGCCATCTAC 
    AAGAGCTCCT GCAGCATCGA CGTCACCTTC TTCCCCTTCG ACCAGCAGAA CTGCAAGATG
    AAGTTTGGCT CCTGGACTTA TGACAAGGCC AAGATCGACC TGGAGCAGAT GGAGCAGACT
    GTGGACCTGA AGGACTACTG GGAGAGCGGC GAGTGGGCCA TCGTCAATGC CACGGGCACC
    TACAACAGCA AGAAGTACGA CTGCTGCGCC GAGATCTACC CCGACGTCAC CTACGCCTTC
    GTCATCCGGC GGCTGCCGCT CTTCTACACC ATCAACCTCA TCATCCCCTG CCTGCTCATC
    TCCTGCCTCA CTGTGCTGGT CTTCTACCTG CCCTCCGACT GCGGCGAGAA GATCACGCTG
    TGCATTTCGG TGCTGCTGTC ACTCACCGTC TTCCTGCTGC TCATCACTGA GATCATCCCG
    TCCACCTCGC TGGTCATCCC GCTCATCGGC GAGTACCTGC TGTTCACCAT GATCTTCGTC
    ACCCTGTCCA TCGTCATCAC CGTCTTCGTG CTCAATGTGC ACCACCGCTC CCCCAGCACC
    CACACCATGC CCCACTGGGT GCGGGGGGCC CTTCTGGGCT GTGTGCCCCG GTGGCTTCTG
    ATGAACCGGC CCCCACCACC CGTGGAGCTC TGCCACCCCC TACGCCTGAA GCTCAGCCCC
    TCTTATCACT GGCTGGAGAG CAACGTGGAT GCCGAGGAGA GGGAGGTGGT GGTGGAGGAG
    GAGGACAGAT GGGCATGTGC AGGTCATGTG GCCCCCTCTG TGGGCACCCT CTGCAGCCAC
    GGCCACCTGC ACTCTGGGGC CTCAGGTCCC AAGGCTGAGG CTCTGCTGCA GGAGGGTGAG
    CTGCTGCTAT CACCCCACAT GCAGAAGGCA CTGGAAGGTG TGCACTACAT TGCCGACCAC
    CTGCGGTCTG AGGATGCTGA CTCTTCGGTG AAGGAGGACT GGAAGTATGT TGCCATGGTC
    ATCGACAGGA TCTTCCTCTG GCTGTTTATC ATCGTCTGCT TCCTGGGGAC CATCGGCCTC
    TTTCTGCCTC CGTTCCTAGC TGGAATGATC TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu56878
    Clone ID Related Accession (Same CDS sequence) XM_011544389.2 , XM_011544389.1 , NM_001347708.2 , NM_001347707.2 , NM_001347708.1 , NM_001347707.1
    Accession Version NM_001347708.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 996bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-10-25
    Organism Homo sapiens(human)
    Product neuronal acetylcholine receptor subunit alpha-2 isoform 4
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA407151.1, AK314787.1 and AF311103.5. On May 31, 2019 this sequence version replaced NM_001347708.1. Transcript Variant: This variant (6), as well as variant 5, encodes isoform 4. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK314787.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN03465411 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    ATGAAGTTTG GCTCCTGGAC TTATGACAAG GCCAAGATCG ACCTGGAGCA GATGGAGCAG 
    ACTGTGGACC TGAAGGACTA CTGGGAGAGC GGCGAGTGGG CCATCGTCAA TGCCACGGGC
    ACCTACAACA GCAAGAAGTA CGACTGCTGC GCCGAGATCT ACCCCGACGT CACCTACGCC
    TTCGTCATCC GGCGGCTGCC GCTCTTCTAC ACCATCAACC TCATCATCCC CTGCCTGCTC
    ATCTCCTGCC TCACTGTGCT GGTCTTCTAC CTGCCCTCCG ACTGCGGCGA GAAGATCACG
    CTGTGCATTT CGGTGCTGCT GTCACTCACC GTCTTCCTGC TGCTCATCAC TGAGATCATC
    CCGTCCACCT CGCTGGTCAT CCCGCTCATC GGCGAGTACC TGCTGTTCAC CATGATCTTC
    GTCACCCTGT CCATCGTCAT CACCGTCTTC GTGCTCAATG TGCACCACCG CTCCCCCAGC
    ACCCACACCA TGCCCCACTG GGTGCGGGGG GCCCTTCTGG GCTGTGTGCC CCGGTGGCTT
    CTGATGAACC GGCCCCCACC ACCCGTGGAG CTCTGCCACC CCCTACGCCT GAAGCTCAGC
    CCCTCTTATC ACTGGCTGGA GAGCAACGTG GATGCCGAGG AGAGGGAGGT GGTGGTGGAG
    GAGGAGGACA GATGGGCATG TGCAGGTCAT GTGGCCCCCT CTGTGGGCAC CCTCTGCAGC
    CACGGCCACC TGCACTCTGG GGCCTCAGGT CCCAAGGCTG AGGCTCTGCT GCAGGAGGGT
    GAGCTGCTGC TATCACCCCA CATGCAGAAG GCACTGGAAG GTGTGCACTA CATTGCCGAC
    CACCTGCGGT CTGAGGATGC TGACTCTTCG GTGAAGGAGG ACTGGAAGTA TGTTGCCATG
    GTCATCGACA GGATCTTCCT CTGGCTGTTT ATCATCGTCT GCTTCCTGGG GACCATCGGC
    CTCTTTCTGC CTCCGTTCCT AGCTGGAATG ATCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001334637.1
    CDS1036..2031
    Translation

    Target ORF information:

    RefSeq Version NM_001347708.2
    Organism Homo sapiens(human)
    Definition Homo sapiens cholinergic receptor nicotinic alpha 2 subunit (CHRNA2), transcript variant 6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001347708.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    ATGAAGTTTG GCTCCTGGAC TTATGACAAG GCCAAGATCG ACCTGGAGCA GATGGAGCAG 
    ACTGTGGACC TGAAGGACTA CTGGGAGAGC GGCGAGTGGG CCATCGTCAA TGCCACGGGC
    ACCTACAACA GCAAGAAGTA CGACTGCTGC GCCGAGATCT ACCCCGACGT CACCTACGCC
    TTCGTCATCC GGCGGCTGCC GCTCTTCTAC ACCATCAACC TCATCATCCC CTGCCTGCTC
    ATCTCCTGCC TCACTGTGCT GGTCTTCTAC CTGCCCTCCG ACTGCGGCGA GAAGATCACG
    CTGTGCATTT CGGTGCTGCT GTCACTCACC GTCTTCCTGC TGCTCATCAC TGAGATCATC
    CCGTCCACCT CGCTGGTCAT CCCGCTCATC GGCGAGTACC TGCTGTTCAC CATGATCTTC
    GTCACCCTGT CCATCGTCAT CACCGTCTTC GTGCTCAATG TGCACCACCG CTCCCCCAGC
    ACCCACACCA TGCCCCACTG GGTGCGGGGG GCCCTTCTGG GCTGTGTGCC CCGGTGGCTT
    CTGATGAACC GGCCCCCACC ACCCGTGGAG CTCTGCCACC CCCTACGCCT GAAGCTCAGC
    CCCTCTTATC ACTGGCTGGA GAGCAACGTG GATGCCGAGG AGAGGGAGGT GGTGGTGGAG
    GAGGAGGACA GATGGGCATG TGCAGGTCAT GTGGCCCCCT CTGTGGGCAC CCTCTGCAGC
    CACGGCCACC TGCACTCTGG GGCCTCAGGT CCCAAGGCTG AGGCTCTGCT GCAGGAGGGT
    GAGCTGCTGC TATCACCCCA CATGCAGAAG GCACTGGAAG GTGTGCACTA CATTGCCGAC
    CACCTGCGGT CTGAGGATGC TGACTCTTCG GTGAAGGAGG ACTGGAAGTA TGTTGCCATG
    GTCATCGACA GGATCTTCCT CTGGCTGTTT ATCATCGTCT GCTTCCTGGG GACCATCGGC
    CTCTTTCTGC CTCCGTTCCT AGCTGGAATG ATCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu56878
    Clone ID Related Accession (Same CDS sequence) XM_011544389.2 , XM_011544389.1 , NM_001347708.2 , NM_001347707.2 , NM_001347708.1 , NM_001347707.1
    Accession Version NM_001347707.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 996bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-10-25
    Organism Homo sapiens(human)
    Product neuronal acetylcholine receptor subunit alpha-2 isoform 4
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AF311103.5. On May 31, 2019 this sequence version replaced NM_001347707.1. Transcript Variant: This variant (5), as well as variant 6, encodes isoform 4. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660809.3654.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2153427, SAMEA2155984 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    ATGAAGTTTG GCTCCTGGAC TTATGACAAG GCCAAGATCG ACCTGGAGCA GATGGAGCAG 
    ACTGTGGACC TGAAGGACTA CTGGGAGAGC GGCGAGTGGG CCATCGTCAA TGCCACGGGC
    ACCTACAACA GCAAGAAGTA CGACTGCTGC GCCGAGATCT ACCCCGACGT CACCTACGCC
    TTCGTCATCC GGCGGCTGCC GCTCTTCTAC ACCATCAACC TCATCATCCC CTGCCTGCTC
    ATCTCCTGCC TCACTGTGCT GGTCTTCTAC CTGCCCTCCG ACTGCGGCGA GAAGATCACG
    CTGTGCATTT CGGTGCTGCT GTCACTCACC GTCTTCCTGC TGCTCATCAC TGAGATCATC
    CCGTCCACCT CGCTGGTCAT CCCGCTCATC GGCGAGTACC TGCTGTTCAC CATGATCTTC
    GTCACCCTGT CCATCGTCAT CACCGTCTTC GTGCTCAATG TGCACCACCG CTCCCCCAGC
    ACCCACACCA TGCCCCACTG GGTGCGGGGG GCCCTTCTGG GCTGTGTGCC CCGGTGGCTT
    CTGATGAACC GGCCCCCACC ACCCGTGGAG CTCTGCCACC CCCTACGCCT GAAGCTCAGC
    CCCTCTTATC ACTGGCTGGA GAGCAACGTG GATGCCGAGG AGAGGGAGGT GGTGGTGGAG
    GAGGAGGACA GATGGGCATG TGCAGGTCAT GTGGCCCCCT CTGTGGGCAC CCTCTGCAGC
    CACGGCCACC TGCACTCTGG GGCCTCAGGT CCCAAGGCTG AGGCTCTGCT GCAGGAGGGT
    GAGCTGCTGC TATCACCCCA CATGCAGAAG GCACTGGAAG GTGTGCACTA CATTGCCGAC
    CACCTGCGGT CTGAGGATGC TGACTCTTCG GTGAAGGAGG ACTGGAAGTA TGTTGCCATG
    GTCATCGACA GGATCTTCCT CTGGCTGTTT ATCATCGTCT GCTTCCTGGG GACCATCGGC
    CTCTTTCTGC CTCCGTTCCT AGCTGGAATG ATCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001334636.1
    CDS1048..2043
    Translation

    Target ORF information:

    RefSeq Version NM_001347707.2
    Organism Homo sapiens(human)
    Definition Homo sapiens cholinergic receptor nicotinic alpha 2 subunit (CHRNA2), transcript variant 5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001347707.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    ATGAAGTTTG GCTCCTGGAC TTATGACAAG GCCAAGATCG ACCTGGAGCA GATGGAGCAG 
    ACTGTGGACC TGAAGGACTA CTGGGAGAGC GGCGAGTGGG CCATCGTCAA TGCCACGGGC
    ACCTACAACA GCAAGAAGTA CGACTGCTGC GCCGAGATCT ACCCCGACGT CACCTACGCC
    TTCGTCATCC GGCGGCTGCC GCTCTTCTAC ACCATCAACC TCATCATCCC CTGCCTGCTC
    ATCTCCTGCC TCACTGTGCT GGTCTTCTAC CTGCCCTCCG ACTGCGGCGA GAAGATCACG
    CTGTGCATTT CGGTGCTGCT GTCACTCACC GTCTTCCTGC TGCTCATCAC TGAGATCATC
    CCGTCCACCT CGCTGGTCAT CCCGCTCATC GGCGAGTACC TGCTGTTCAC CATGATCTTC
    GTCACCCTGT CCATCGTCAT CACCGTCTTC GTGCTCAATG TGCACCACCG CTCCCCCAGC
    ACCCACACCA TGCCCCACTG GGTGCGGGGG GCCCTTCTGG GCTGTGTGCC CCGGTGGCTT
    CTGATGAACC GGCCCCCACC ACCCGTGGAG CTCTGCCACC CCCTACGCCT GAAGCTCAGC
    CCCTCTTATC ACTGGCTGGA GAGCAACGTG GATGCCGAGG AGAGGGAGGT GGTGGTGGAG
    GAGGAGGACA GATGGGCATG TGCAGGTCAT GTGGCCCCCT CTGTGGGCAC CCTCTGCAGC
    CACGGCCACC TGCACTCTGG GGCCTCAGGT CCCAAGGCTG AGGCTCTGCT GCAGGAGGGT
    GAGCTGCTGC TATCACCCCA CATGCAGAAG GCACTGGAAG GTGTGCACTA CATTGCCGAC
    CACCTGCGGT CTGAGGATGC TGACTCTTCG GTGAAGGAGG ACTGGAAGTA TGTTGCCATG
    GTCATCGACA GGATCTTCCT CTGGCTGTTT ATCATCGTCT GCTTCCTGGG GACCATCGGC
    CTCTTTCTGC CTCCGTTCCT AGCTGGAATG ATCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu56878
    Clone ID Related Accession (Same CDS sequence) XM_011544389.2 , XM_011544389.1 , NM_001347708.2 , NM_001347707.2 , NM_001347708.1 , NM_001347707.1
    Accession Version NM_001347708.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 996bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-02-25
    Organism Homo sapiens(human)
    Product neuronal acetylcholine receptor subunit alpha-2 isoform 4
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA407151.1, AK314787.1 and AF311103.5. Transcript Variant: This variant (6), as well as variant 5, encodes isoform 4. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK314787.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN03465411 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    ATGAAGTTTG GCTCCTGGAC TTATGACAAG GCCAAGATCG ACCTGGAGCA GATGGAGCAG 
    ACTGTGGACC TGAAGGACTA CTGGGAGAGC GGCGAGTGGG CCATCGTCAA TGCCACGGGC
    ACCTACAACA GCAAGAAGTA CGACTGCTGC GCCGAGATCT ACCCCGACGT CACCTACGCC
    TTCGTCATCC GGCGGCTGCC GCTCTTCTAC ACCATCAACC TCATCATCCC CTGCCTGCTC
    ATCTCCTGCC TCACTGTGCT GGTCTTCTAC CTGCCCTCCG ACTGCGGCGA GAAGATCACG
    CTGTGCATTT CGGTGCTGCT GTCACTCACC GTCTTCCTGC TGCTCATCAC TGAGATCATC
    CCGTCCACCT CGCTGGTCAT CCCGCTCATC GGCGAGTACC TGCTGTTCAC CATGATCTTC
    GTCACCCTGT CCATCGTCAT CACCGTCTTC GTGCTCAATG TGCACCACCG CTCCCCCAGC
    ACCCACACCA TGCCCCACTG GGTGCGGGGG GCCCTTCTGG GCTGTGTGCC CCGGTGGCTT
    CTGATGAACC GGCCCCCACC ACCCGTGGAG CTCTGCCACC CCCTACGCCT GAAGCTCAGC
    CCCTCTTATC ACTGGCTGGA GAGCAACGTG GATGCCGAGG AGAGGGAGGT GGTGGTGGAG
    GAGGAGGACA GATGGGCATG TGCAGGTCAT GTGGCCCCCT CTGTGGGCAC CCTCTGCAGC
    CACGGCCACC TGCACTCTGG GGCCTCAGGT CCCAAGGCTG AGGCTCTGCT GCAGGAGGGT
    GAGCTGCTGC TATCACCCCA CATGCAGAAG GCACTGGAAG GTGTGCACTA CATTGCCGAC
    CACCTGCGGT CTGAGGATGC TGACTCTTCG GTGAAGGAGG ACTGGAAGTA TGTTGCCATG
    GTCATCGACA GGATCTTCCT CTGGCTGTTT ATCATCGTCT GCTTCCTGGG GACCATCGGC
    CTCTTTCTGC CTCCGTTCCT AGCTGGAATG ATCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001334637.1
    CDS1071..2066
    Translation

    Target ORF information:

    RefSeq Version NM_001347708.1
    Organism Homo sapiens(human)
    Definition Homo sapiens cholinergic receptor nicotinic alpha 2 subunit (CHRNA2), transcript variant 6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001347708.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    ATGAAGTTTG GCTCCTGGAC TTATGACAAG GCCAAGATCG ACCTGGAGCA GATGGAGCAG 
    ACTGTGGACC TGAAGGACTA CTGGGAGAGC GGCGAGTGGG CCATCGTCAA TGCCACGGGC
    ACCTACAACA GCAAGAAGTA CGACTGCTGC GCCGAGATCT ACCCCGACGT CACCTACGCC
    TTCGTCATCC GGCGGCTGCC GCTCTTCTAC ACCATCAACC TCATCATCCC CTGCCTGCTC
    ATCTCCTGCC TCACTGTGCT GGTCTTCTAC CTGCCCTCCG ACTGCGGCGA GAAGATCACG
    CTGTGCATTT CGGTGCTGCT GTCACTCACC GTCTTCCTGC TGCTCATCAC TGAGATCATC
    CCGTCCACCT CGCTGGTCAT CCCGCTCATC GGCGAGTACC TGCTGTTCAC CATGATCTTC
    GTCACCCTGT CCATCGTCAT CACCGTCTTC GTGCTCAATG TGCACCACCG CTCCCCCAGC
    ACCCACACCA TGCCCCACTG GGTGCGGGGG GCCCTTCTGG GCTGTGTGCC CCGGTGGCTT
    CTGATGAACC GGCCCCCACC ACCCGTGGAG CTCTGCCACC CCCTACGCCT GAAGCTCAGC
    CCCTCTTATC ACTGGCTGGA GAGCAACGTG GATGCCGAGG AGAGGGAGGT GGTGGTGGAG
    GAGGAGGACA GATGGGCATG TGCAGGTCAT GTGGCCCCCT CTGTGGGCAC CCTCTGCAGC
    CACGGCCACC TGCACTCTGG GGCCTCAGGT CCCAAGGCTG AGGCTCTGCT GCAGGAGGGT
    GAGCTGCTGC TATCACCCCA CATGCAGAAG GCACTGGAAG GTGTGCACTA CATTGCCGAC
    CACCTGCGGT CTGAGGATGC TGACTCTTCG GTGAAGGAGG ACTGGAAGTA TGTTGCCATG
    GTCATCGACA GGATCTTCCT CTGGCTGTTT ATCATCGTCT GCTTCCTGGG GACCATCGGC
    CTCTTTCTGC CTCCGTTCCT AGCTGGAATG ATCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu56878
    Clone ID Related Accession (Same CDS sequence) XM_011544389.2 , XM_011544389.1 , NM_001347708.2 , NM_001347707.2 , NM_001347708.1 , NM_001347707.1
    Accession Version NM_001347707.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 996bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-02-25
    Organism Homo sapiens(human)
    Product neuronal acetylcholine receptor subunit alpha-2 isoform 4
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AF311103.5. On Dec 9, 2016 this sequence version replaced XM_017013004.1. Transcript Variant: This variant (5), as well as variant 6, encodes isoform 4. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660809.3654.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2153427, SAMEA2155984 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    ATGAAGTTTG GCTCCTGGAC TTATGACAAG GCCAAGATCG ACCTGGAGCA GATGGAGCAG 
    ACTGTGGACC TGAAGGACTA CTGGGAGAGC GGCGAGTGGG CCATCGTCAA TGCCACGGGC
    ACCTACAACA GCAAGAAGTA CGACTGCTGC GCCGAGATCT ACCCCGACGT CACCTACGCC
    TTCGTCATCC GGCGGCTGCC GCTCTTCTAC ACCATCAACC TCATCATCCC CTGCCTGCTC
    ATCTCCTGCC TCACTGTGCT GGTCTTCTAC CTGCCCTCCG ACTGCGGCGA GAAGATCACG
    CTGTGCATTT CGGTGCTGCT GTCACTCACC GTCTTCCTGC TGCTCATCAC TGAGATCATC
    CCGTCCACCT CGCTGGTCAT CCCGCTCATC GGCGAGTACC TGCTGTTCAC CATGATCTTC
    GTCACCCTGT CCATCGTCAT CACCGTCTTC GTGCTCAATG TGCACCACCG CTCCCCCAGC
    ACCCACACCA TGCCCCACTG GGTGCGGGGG GCCCTTCTGG GCTGTGTGCC CCGGTGGCTT
    CTGATGAACC GGCCCCCACC ACCCGTGGAG CTCTGCCACC CCCTACGCCT GAAGCTCAGC
    CCCTCTTATC ACTGGCTGGA GAGCAACGTG GATGCCGAGG AGAGGGAGGT GGTGGTGGAG
    GAGGAGGACA GATGGGCATG TGCAGGTCAT GTGGCCCCCT CTGTGGGCAC CCTCTGCAGC
    CACGGCCACC TGCACTCTGG GGCCTCAGGT CCCAAGGCTG AGGCTCTGCT GCAGGAGGGT
    GAGCTGCTGC TATCACCCCA CATGCAGAAG GCACTGGAAG GTGTGCACTA CATTGCCGAC
    CACCTGCGGT CTGAGGATGC TGACTCTTCG GTGAAGGAGG ACTGGAAGTA TGTTGCCATG
    GTCATCGACA GGATCTTCCT CTGGCTGTTT ATCATCGTCT GCTTCCTGGG GACCATCGGC
    CTCTTTCTGC CTCCGTTCCT AGCTGGAATG ATCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001334636.1
    CDS1083..2078
    Translation

    Target ORF information:

    RefSeq Version NM_001347707.1
    Organism Homo sapiens(human)
    Definition Homo sapiens cholinergic receptor nicotinic alpha 2 subunit (CHRNA2), transcript variant 5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001347707.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    ATGAAGTTTG GCTCCTGGAC TTATGACAAG GCCAAGATCG ACCTGGAGCA GATGGAGCAG 
    ACTGTGGACC TGAAGGACTA CTGGGAGAGC GGCGAGTGGG CCATCGTCAA TGCCACGGGC
    ACCTACAACA GCAAGAAGTA CGACTGCTGC GCCGAGATCT ACCCCGACGT CACCTACGCC
    TTCGTCATCC GGCGGCTGCC GCTCTTCTAC ACCATCAACC TCATCATCCC CTGCCTGCTC
    ATCTCCTGCC TCACTGTGCT GGTCTTCTAC CTGCCCTCCG ACTGCGGCGA GAAGATCACG
    CTGTGCATTT CGGTGCTGCT GTCACTCACC GTCTTCCTGC TGCTCATCAC TGAGATCATC
    CCGTCCACCT CGCTGGTCAT CCCGCTCATC GGCGAGTACC TGCTGTTCAC CATGATCTTC
    GTCACCCTGT CCATCGTCAT CACCGTCTTC GTGCTCAATG TGCACCACCG CTCCCCCAGC
    ACCCACACCA TGCCCCACTG GGTGCGGGGG GCCCTTCTGG GCTGTGTGCC CCGGTGGCTT
    CTGATGAACC GGCCCCCACC ACCCGTGGAG CTCTGCCACC CCCTACGCCT GAAGCTCAGC
    CCCTCTTATC ACTGGCTGGA GAGCAACGTG GATGCCGAGG AGAGGGAGGT GGTGGTGGAG
    GAGGAGGACA GATGGGCATG TGCAGGTCAT GTGGCCCCCT CTGTGGGCAC CCTCTGCAGC
    CACGGCCACC TGCACTCTGG GGCCTCAGGT CCCAAGGCTG AGGCTCTGCT GCAGGAGGGT
    GAGCTGCTGC TATCACCCCA CATGCAGAAG GCACTGGAAG GTGTGCACTA CATTGCCGAC
    CACCTGCGGT CTGAGGATGC TGACTCTTCG GTGAAGGAGG ACTGGAAGTA TGTTGCCATG
    GTCATCGACA GGATCTTCCT CTGGCTGTTT ATCATCGTCT GCTTCCTGGG GACCATCGGC
    CTCTTTCTGC CTCCGTTCCT AGCTGGAATG ATCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu56878
    Clone ID Related Accession (Same CDS sequence) XM_011544389.2 , XM_011544389.1 , NM_001347708.2 , NM_001347707.2 , NM_001347708.1 , NM_001347707.1
    Accession Version XM_011544389.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 996bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product neuronal acetylcholine receptor subunit alpha-2 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000008.11) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_011544389.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    ATGAAGTTTG GCTCCTGGAC TTATGACAAG GCCAAGATCG ACCTGGAGCA GATGGAGCAG 
    ACTGTGGACC TGAAGGACTA CTGGGAGAGC GGCGAGTGGG CCATCGTCAA TGCCACGGGC
    ACCTACAACA GCAAGAAGTA CGACTGCTGC GCCGAGATCT ACCCCGACGT CACCTACGCC
    TTCGTCATCC GGCGGCTGCC GCTCTTCTAC ACCATCAACC TCATCATCCC CTGCCTGCTC
    ATCTCCTGCC TCACTGTGCT GGTCTTCTAC CTGCCCTCCG ACTGCGGCGA GAAGATCACG
    CTGTGCATTT CGGTGCTGCT GTCACTCACC GTCTTCCTGC TGCTCATCAC TGAGATCATC
    CCGTCCACCT CGCTGGTCAT CCCGCTCATC GGCGAGTACC TGCTGTTCAC CATGATCTTC
    GTCACCCTGT CCATCGTCAT CACCGTCTTC GTGCTCAATG TGCACCACCG CTCCCCCAGC
    ACCCACACCA TGCCCCACTG GGTGCGGGGG GCCCTTCTGG GCTGTGTGCC CCGGTGGCTT
    CTGATGAACC GGCCCCCACC ACCCGTGGAG CTCTGCCACC CCCTACGCCT GAAGCTCAGC
    CCCTCTTATC ACTGGCTGGA GAGCAACGTG GATGCCGAGG AGAGGGAGGT GGTGGTGGAG
    GAGGAGGACA GATGGGCATG TGCAGGTCAT GTGGCCCCCT CTGTGGGCAC CCTCTGCAGC
    CACGGCCACC TGCACTCTGG GGCCTCAGGT CCCAAGGCTG AGGCTCTGCT GCAGGAGGGT
    GAGCTGCTGC TATCACCCCA CATGCAGAAG GCACTGGAAG GTGTGCACTA CATTGCCGAC
    CACCTGCGGT CTGAGGATGC TGACTCTTCG GTGAAGGAGG ACTGGAAGTA TGTTGCCATG
    GTCATCGACA GGATCTTCCT CTGGCTGTTT ATCATCGTCT GCTTCCTGGG GACCATCGGC
    CTCTTTCTGC CTCCGTTCCT AGCTGGAATG ATCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_011542691.1
    CDS1057..2052
    Translation

    Target ORF information:

    RefSeq Version XM_011544389.2
    Organism Homo sapiens(human)
    Definition Homo sapiens cholinergic receptor nicotinic alpha 2 subunit (CHRNA2), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_011544389.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    ATGAAGTTTG GCTCCTGGAC TTATGACAAG GCCAAGATCG ACCTGGAGCA GATGGAGCAG 
    ACTGTGGACC TGAAGGACTA CTGGGAGAGC GGCGAGTGGG CCATCGTCAA TGCCACGGGC
    ACCTACAACA GCAAGAAGTA CGACTGCTGC GCCGAGATCT ACCCCGACGT CACCTACGCC
    TTCGTCATCC GGCGGCTGCC GCTCTTCTAC ACCATCAACC TCATCATCCC CTGCCTGCTC
    ATCTCCTGCC TCACTGTGCT GGTCTTCTAC CTGCCCTCCG ACTGCGGCGA GAAGATCACG
    CTGTGCATTT CGGTGCTGCT GTCACTCACC GTCTTCCTGC TGCTCATCAC TGAGATCATC
    CCGTCCACCT CGCTGGTCAT CCCGCTCATC GGCGAGTACC TGCTGTTCAC CATGATCTTC
    GTCACCCTGT CCATCGTCAT CACCGTCTTC GTGCTCAATG TGCACCACCG CTCCCCCAGC
    ACCCACACCA TGCCCCACTG GGTGCGGGGG GCCCTTCTGG GCTGTGTGCC CCGGTGGCTT
    CTGATGAACC GGCCCCCACC ACCCGTGGAG CTCTGCCACC CCCTACGCCT GAAGCTCAGC
    CCCTCTTATC ACTGGCTGGA GAGCAACGTG GATGCCGAGG AGAGGGAGGT GGTGGTGGAG
    GAGGAGGACA GATGGGCATG TGCAGGTCAT GTGGCCCCCT CTGTGGGCAC CCTCTGCAGC
    CACGGCCACC TGCACTCTGG GGCCTCAGGT CCCAAGGCTG AGGCTCTGCT GCAGGAGGGT
    GAGCTGCTGC TATCACCCCA CATGCAGAAG GCACTGGAAG GTGTGCACTA CATTGCCGAC
    CACCTGCGGT CTGAGGATGC TGACTCTTCG GTGAAGGAGG ACTGGAAGTA TGTTGCCATG
    GTCATCGACA GGATCTTCCT CTGGCTGTTT ATCATCGTCT GCTTCCTGGG GACCATCGGC
    CTCTTTCTGC CTCCGTTCCT AGCTGGAATG ATCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu56878
    Clone ID Related Accession (Same CDS sequence) XM_011544389.2 , XM_011544389.1 , NM_001347708.2 , NM_001347707.2 , NM_001347708.1 , NM_001347707.1
    Accession Version XM_011544389.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 996bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-05
    Organism Homo sapiens(human)
    Product neuronal acetylcholine receptor subunit alpha-2 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000008.11) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    ATGAAGTTTG GCTCCTGGAC TTATGACAAG GCCAAGATCG ACCTGGAGCA GATGGAGCAG 
    ACTGTGGACC TGAAGGACTA CTGGGAGAGC GGCGAGTGGG CCATCGTCAA TGCCACGGGC
    ACCTACAACA GCAAGAAGTA CGACTGCTGC GCCGAGATCT ACCCCGACGT CACCTACGCC
    TTCGTCATCC GGCGGCTGCC GCTCTTCTAC ACCATCAACC TCATCATCCC CTGCCTGCTC
    ATCTCCTGCC TCACTGTGCT GGTCTTCTAC CTGCCCTCCG ACTGCGGCGA GAAGATCACG
    CTGTGCATTT CGGTGCTGCT GTCACTCACC GTCTTCCTGC TGCTCATCAC TGAGATCATC
    CCGTCCACCT CGCTGGTCAT CCCGCTCATC GGCGAGTACC TGCTGTTCAC CATGATCTTC
    GTCACCCTGT CCATCGTCAT CACCGTCTTC GTGCTCAATG TGCACCACCG CTCCCCCAGC
    ACCCACACCA TGCCCCACTG GGTGCGGGGG GCCCTTCTGG GCTGTGTGCC CCGGTGGCTT
    CTGATGAACC GGCCCCCACC ACCCGTGGAG CTCTGCCACC CCCTACGCCT GAAGCTCAGC
    CCCTCTTATC ACTGGCTGGA GAGCAACGTG GATGCCGAGG AGAGGGAGGT GGTGGTGGAG
    GAGGAGGACA GATGGGCATG TGCAGGTCAT GTGGCCCCCT CTGTGGGCAC CCTCTGCAGC
    CACGGCCACC TGCACTCTGG GGCCTCAGGT CCCAAGGCTG AGGCTCTGCT GCAGGAGGGT
    GAGCTGCTGC TATCACCCCA CATGCAGAAG GCACTGGAAG GTGTGCACTA CATTGCCGAC
    CACCTGCGGT CTGAGGATGC TGACTCTTCG GTGAAGGAGG ACTGGAAGTA TGTTGCCATG
    GTCATCGACA GGATCTTCCT CTGGCTGTTT ATCATCGTCT GCTTCCTGGG GACCATCGGC
    CTCTTTCTGC CTCCGTTCCT AGCTGGAATG ATCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_011542691.1
    CDS1068..2063
    Translation

    Target ORF information:

    RefSeq Version XM_011544389.1
    Organism Homo sapiens(human)
    Definition Homo sapiens cholinergic receptor nicotinic alpha 2 subunit (CHRNA2), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_011544389.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    ATGAAGTTTG GCTCCTGGAC TTATGACAAG GCCAAGATCG ACCTGGAGCA GATGGAGCAG 
    ACTGTGGACC TGAAGGACTA CTGGGAGAGC GGCGAGTGGG CCATCGTCAA TGCCACGGGC
    ACCTACAACA GCAAGAAGTA CGACTGCTGC GCCGAGATCT ACCCCGACGT CACCTACGCC
    TTCGTCATCC GGCGGCTGCC GCTCTTCTAC ACCATCAACC TCATCATCCC CTGCCTGCTC
    ATCTCCTGCC TCACTGTGCT GGTCTTCTAC CTGCCCTCCG ACTGCGGCGA GAAGATCACG
    CTGTGCATTT CGGTGCTGCT GTCACTCACC GTCTTCCTGC TGCTCATCAC TGAGATCATC
    CCGTCCACCT CGCTGGTCAT CCCGCTCATC GGCGAGTACC TGCTGTTCAC CATGATCTTC
    GTCACCCTGT CCATCGTCAT CACCGTCTTC GTGCTCAATG TGCACCACCG CTCCCCCAGC
    ACCCACACCA TGCCCCACTG GGTGCGGGGG GCCCTTCTGG GCTGTGTGCC CCGGTGGCTT
    CTGATGAACC GGCCCCCACC ACCCGTGGAG CTCTGCCACC CCCTACGCCT GAAGCTCAGC
    CCCTCTTATC ACTGGCTGGA GAGCAACGTG GATGCCGAGG AGAGGGAGGT GGTGGTGGAG
    GAGGAGGACA GATGGGCATG TGCAGGTCAT GTGGCCCCCT CTGTGGGCAC CCTCTGCAGC
    CACGGCCACC TGCACTCTGG GGCCTCAGGT CCCAAGGCTG AGGCTCTGCT GCAGGAGGGT
    GAGCTGCTGC TATCACCCCA CATGCAGAAG GCACTGGAAG GTGTGCACTA CATTGCCGAC
    CACCTGCGGT CTGAGGATGC TGACTCTTCG GTGAAGGAGG ACTGGAAGTA TGTTGCCATG
    GTCATCGACA GGATCTTCCT CTGGCTGTTT ATCATCGTCT GCTTCCTGGG GACCATCGGC
    CTCTTTCTGC CTCCGTTCCT AGCTGGAATG ATCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu22359
    Clone ID Related Accession (Same CDS sequence) NM_001282455.1 , NM_001282455.2
    Accession Version NM_001282455.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1545bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-02-23
    Organism Homo sapiens(human)
    Product neuronal acetylcholine receptor subunit alpha-2 isoform 2 precursor
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AF311103.5, AK296348.1 and AW292180.1. On Sep 7, 2013 this sequence version replaced XM_005273396.1. Transcript Variant: This variant (2) uses an alternate in-frame splice site compared to variant 1. The encoded isoform (2) is shorter than isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803615.90046.1, AK296348.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2153427, SAMEA2155984 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    ATGGGCCCCT CCTGTCCTGT GTTCCTGTCC TTCACAAAGC TCAGCCTGTG GTGGCTCCTT 
    CTGACCCCAG CAGGTGGAGA GGAAGCTAAG CGCCCACCTC CCAGGGCTCC TGGAGACCCA
    CTCTCCTCTC CCAGTCCCAC GGCATTGCCG CAGGGAGGCT CGCATACCGA GACTGAGGAC
    CGGCTCTTCA AACACCTCTT CCGGGGCTAC AACCGCTGGG CGCGCCCGGT GCCCAACACT
    TCAGACGTGG ATGAGAAGAA CCAAATGATG ACCACCAACG TCTGGCTAAA ACAGGAGTGG
    AGCGACTACA AACTGCGCTG GAACCCCACT GATTTTGGCA ACATCACATC TCTCAGGGTC
    CCTTCTGAGA TGATCTGGAT CCCCGACATT GTTCTCTACA ACAATGCAGA TGGGGAGTTT
    GCAGTGACCC ACATGACCAA GGCCCACCTC TTCTCCACGG GCACTGTGCA CTGGGTGCCC
    CCGGCCATCT ACAAGAGCTC CTGCAGCATC GACGTCACCT TCTTCCCCTT CGACCAGCAG
    AACTGCAAGA TGAAGTTTGG CTCCTGGACT TATGACAAGG CCAAGATCGA CCTGGAGCAG
    ATGGAGCAGA CTGTGGACCT GAAGGACTAC TGGGAGAGCG GCGAGTGGGC CATCGTCAAT
    GCCACGGGCA CCTACAACAG CAAGAAGTAC GACTGCTGCG CCGAGATCTA CCCCGACGTC
    ACCTACGCCT TCGTCATCCG GCGGCTGCCG CTCTTCTACA CCATCAACCT CATCATCCCC
    TGCCTGCTCA TCTCCTGCCT CACTGTGCTG GTCTTCTACC TGCCCTCCGA CTGCGGCGAG
    AAGATCACGC TGTGCATTTC GGTGCTGCTG TCACTCACCG TCTTCCTGCT GCTCATCACT
    GAGATCATCC CGTCCACCTC GCTGGTCATC CCGCTCATCG GCGAGTACCT GCTGTTCACC
    ATGATCTTCG TCACCCTGTC CATCGTCATC ACCGTCTTCG TGCTCAATGT GCACCACCGC
    TCCCCCAGCA CCCACACCAT GCCCCACTGG GTGCGGGGGG CCCTTCTGGG CTGTGTGCCC
    CGGTGGCTTC TGATGAACCG GCCCCCACCA CCCGTGGAGC TCTGCCACCC CCTACGCCTG
    AAGCTCAGCC CCTCTTATCA CTGGCTGGAG AGCAACGTGG ATGCCGAGGA GAGGGAGGTG
    GTGGTGGAGG AGGAGGACAG ATGGGCATGT GCAGGTCATG TGGCCCCCTC TGTGGGCACC
    CTCTGCAGCC ACGGCCACCT GCACTCTGGG GCCTCAGGTC CCAAGGCTGA GGCTCTGCTG
    CAGGAGGGTG AGCTGCTGCT ATCACCCCAC ATGCAGAAGG CACTGGAAGG TGTGCACTAC
    ATTGCCGACC ACCTGCGGTC TGAGGATGCT GACTCTTCGG TGAAGGAGGA CTGGAAGTAT
    GTTGCCATGG TCATCGACAG GATCTTCCTC TGGCTGTTTA TCATCGTCTG CTTCCTGGGG
    ACCATCGGCC TCTTTCTGCC TCCGTTCCTA GCTGGAATGA TCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001269384.1
    CDS610..2154
    Translation

    Target ORF information:

    RefSeq Version NM_001282455.1
    Organism Homo sapiens(human)
    Definition Homo sapiens cholinergic receptor nicotinic alpha 2 subunit (CHRNA2), transcript variant 2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001282455.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    ATGGGCCCCT CCTGTCCTGT GTTCCTGTCC TTCACAAAGC TCAGCCTGTG GTGGCTCCTT 
    CTGACCCCAG CAGGTGGAGA GGAAGCTAAG CGCCCACCTC CCAGGGCTCC TGGAGACCCA
    CTCTCCTCTC CCAGTCCCAC GGCATTGCCG CAGGGAGGCT CGCATACCGA GACTGAGGAC
    CGGCTCTTCA AACACCTCTT CCGGGGCTAC AACCGCTGGG CGCGCCCGGT GCCCAACACT
    TCAGACGTGG ATGAGAAGAA CCAAATGATG ACCACCAACG TCTGGCTAAA ACAGGAGTGG
    AGCGACTACA AACTGCGCTG GAACCCCACT GATTTTGGCA ACATCACATC TCTCAGGGTC
    CCTTCTGAGA TGATCTGGAT CCCCGACATT GTTCTCTACA ACAATGCAGA TGGGGAGTTT
    GCAGTGACCC ACATGACCAA GGCCCACCTC TTCTCCACGG GCACTGTGCA CTGGGTGCCC
    CCGGCCATCT ACAAGAGCTC CTGCAGCATC GACGTCACCT TCTTCCCCTT CGACCAGCAG
    AACTGCAAGA TGAAGTTTGG CTCCTGGACT TATGACAAGG CCAAGATCGA CCTGGAGCAG
    ATGGAGCAGA CTGTGGACCT GAAGGACTAC TGGGAGAGCG GCGAGTGGGC CATCGTCAAT
    GCCACGGGCA CCTACAACAG CAAGAAGTAC GACTGCTGCG CCGAGATCTA CCCCGACGTC
    ACCTACGCCT TCGTCATCCG GCGGCTGCCG CTCTTCTACA CCATCAACCT CATCATCCCC
    TGCCTGCTCA TCTCCTGCCT CACTGTGCTG GTCTTCTACC TGCCCTCCGA CTGCGGCGAG
    AAGATCACGC TGTGCATTTC GGTGCTGCTG TCACTCACCG TCTTCCTGCT GCTCATCACT
    GAGATCATCC CGTCCACCTC GCTGGTCATC CCGCTCATCG GCGAGTACCT GCTGTTCACC
    ATGATCTTCG TCACCCTGTC CATCGTCATC ACCGTCTTCG TGCTCAATGT GCACCACCGC
    TCCCCCAGCA CCCACACCAT GCCCCACTGG GTGCGGGGGG CCCTTCTGGG CTGTGTGCCC
    CGGTGGCTTC TGATGAACCG GCCCCCACCA CCCGTGGAGC TCTGCCACCC CCTACGCCTG
    AAGCTCAGCC CCTCTTATCA CTGGCTGGAG AGCAACGTGG ATGCCGAGGA GAGGGAGGTG
    GTGGTGGAGG AGGAGGACAG ATGGGCATGT GCAGGTCATG TGGCCCCCTC TGTGGGCACC
    CTCTGCAGCC ACGGCCACCT GCACTCTGGG GCCTCAGGTC CCAAGGCTGA GGCTCTGCTG
    CAGGAGGGTG AGCTGCTGCT ATCACCCCAC ATGCAGAAGG CACTGGAAGG TGTGCACTAC
    ATTGCCGACC ACCTGCGGTC TGAGGATGCT GACTCTTCGG TGAAGGAGGA CTGGAAGTAT
    GTTGCCATGG TCATCGACAG GATCTTCCTC TGGCTGTTTA TCATCGTCTG CTTCCTGGGG
    ACCATCGGCC TCTTTCTGCC TCCGTTCCTA GCTGGAATGA TCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu22359
    Clone ID Related Accession (Same CDS sequence) NM_001282455.1 , NM_001282455.2
    Accession Version NM_001282455.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1545bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-10-25
    Organism Homo sapiens(human)
    Product neuronal acetylcholine receptor subunit alpha-2 isoform 2 precursor
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AF311103.5, AK296348.1 and AW292180.1. On May 31, 2019 this sequence version replaced NM_001282455.1. Transcript Variant: This variant (2) uses an alternate in-frame splice site compared to variant 1. The encoded isoform (2) is shorter than isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803615.90046.1, AK296348.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2153427, SAMEA2155984 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    ATGGGCCCCT CCTGTCCTGT GTTCCTGTCC TTCACAAAGC TCAGCCTGTG GTGGCTCCTT 
    CTGACCCCAG CAGGTGGAGA GGAAGCTAAG CGCCCACCTC CCAGGGCTCC TGGAGACCCA
    CTCTCCTCTC CCAGTCCCAC GGCATTGCCG CAGGGAGGCT CGCATACCGA GACTGAGGAC
    CGGCTCTTCA AACACCTCTT CCGGGGCTAC AACCGCTGGG CGCGCCCGGT GCCCAACACT
    TCAGACGTGG ATGAGAAGAA CCAAATGATG ACCACCAACG TCTGGCTAAA ACAGGAGTGG
    AGCGACTACA AACTGCGCTG GAACCCCACT GATTTTGGCA ACATCACATC TCTCAGGGTC
    CCTTCTGAGA TGATCTGGAT CCCCGACATT GTTCTCTACA ACAATGCAGA TGGGGAGTTT
    GCAGTGACCC ACATGACCAA GGCCCACCTC TTCTCCACGG GCACTGTGCA CTGGGTGCCC
    CCGGCCATCT ACAAGAGCTC CTGCAGCATC GACGTCACCT TCTTCCCCTT CGACCAGCAG
    AACTGCAAGA TGAAGTTTGG CTCCTGGACT TATGACAAGG CCAAGATCGA CCTGGAGCAG
    ATGGAGCAGA CTGTGGACCT GAAGGACTAC TGGGAGAGCG GCGAGTGGGC CATCGTCAAT
    GCCACGGGCA CCTACAACAG CAAGAAGTAC GACTGCTGCG CCGAGATCTA CCCCGACGTC
    ACCTACGCCT TCGTCATCCG GCGGCTGCCG CTCTTCTACA CCATCAACCT CATCATCCCC
    TGCCTGCTCA TCTCCTGCCT CACTGTGCTG GTCTTCTACC TGCCCTCCGA CTGCGGCGAG
    AAGATCACGC TGTGCATTTC GGTGCTGCTG TCACTCACCG TCTTCCTGCT GCTCATCACT
    GAGATCATCC CGTCCACCTC GCTGGTCATC CCGCTCATCG GCGAGTACCT GCTGTTCACC
    ATGATCTTCG TCACCCTGTC CATCGTCATC ACCGTCTTCG TGCTCAATGT GCACCACCGC
    TCCCCCAGCA CCCACACCAT GCCCCACTGG GTGCGGGGGG CCCTTCTGGG CTGTGTGCCC
    CGGTGGCTTC TGATGAACCG GCCCCCACCA CCCGTGGAGC TCTGCCACCC CCTACGCCTG
    AAGCTCAGCC CCTCTTATCA CTGGCTGGAG AGCAACGTGG ATGCCGAGGA GAGGGAGGTG
    GTGGTGGAGG AGGAGGACAG ATGGGCATGT GCAGGTCATG TGGCCCCCTC TGTGGGCACC
    CTCTGCAGCC ACGGCCACCT GCACTCTGGG GCCTCAGGTC CCAAGGCTGA GGCTCTGCTG
    CAGGAGGGTG AGCTGCTGCT ATCACCCCAC ATGCAGAAGG CACTGGAAGG TGTGCACTAC
    ATTGCCGACC ACCTGCGGTC TGAGGATGCT GACTCTTCGG TGAAGGAGGA CTGGAAGTAT
    GTTGCCATGG TCATCGACAG GATCTTCCTC TGGCTGTTTA TCATCGTCTG CTTCCTGGGG
    ACCATCGGCC TCTTTCTGCC TCCGTTCCTA GCTGGAATGA TCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001269384.1
    CDS575..2119
    Translation

    Target ORF information:

    RefSeq Version NM_001282455.2
    Organism Homo sapiens(human)
    Definition Homo sapiens cholinergic receptor nicotinic alpha 2 subunit (CHRNA2), transcript variant 2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001282455.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    ATGGGCCCCT CCTGTCCTGT GTTCCTGTCC TTCACAAAGC TCAGCCTGTG GTGGCTCCTT 
    CTGACCCCAG CAGGTGGAGA GGAAGCTAAG CGCCCACCTC CCAGGGCTCC TGGAGACCCA
    CTCTCCTCTC CCAGTCCCAC GGCATTGCCG CAGGGAGGCT CGCATACCGA GACTGAGGAC
    CGGCTCTTCA AACACCTCTT CCGGGGCTAC AACCGCTGGG CGCGCCCGGT GCCCAACACT
    TCAGACGTGG ATGAGAAGAA CCAAATGATG ACCACCAACG TCTGGCTAAA ACAGGAGTGG
    AGCGACTACA AACTGCGCTG GAACCCCACT GATTTTGGCA ACATCACATC TCTCAGGGTC
    CCTTCTGAGA TGATCTGGAT CCCCGACATT GTTCTCTACA ACAATGCAGA TGGGGAGTTT
    GCAGTGACCC ACATGACCAA GGCCCACCTC TTCTCCACGG GCACTGTGCA CTGGGTGCCC
    CCGGCCATCT ACAAGAGCTC CTGCAGCATC GACGTCACCT TCTTCCCCTT CGACCAGCAG
    AACTGCAAGA TGAAGTTTGG CTCCTGGACT TATGACAAGG CCAAGATCGA CCTGGAGCAG
    ATGGAGCAGA CTGTGGACCT GAAGGACTAC TGGGAGAGCG GCGAGTGGGC CATCGTCAAT
    GCCACGGGCA CCTACAACAG CAAGAAGTAC GACTGCTGCG CCGAGATCTA CCCCGACGTC
    ACCTACGCCT TCGTCATCCG GCGGCTGCCG CTCTTCTACA CCATCAACCT CATCATCCCC
    TGCCTGCTCA TCTCCTGCCT CACTGTGCTG GTCTTCTACC TGCCCTCCGA CTGCGGCGAG
    AAGATCACGC TGTGCATTTC GGTGCTGCTG TCACTCACCG TCTTCCTGCT GCTCATCACT
    GAGATCATCC CGTCCACCTC GCTGGTCATC CCGCTCATCG GCGAGTACCT GCTGTTCACC
    ATGATCTTCG TCACCCTGTC CATCGTCATC ACCGTCTTCG TGCTCAATGT GCACCACCGC
    TCCCCCAGCA CCCACACCAT GCCCCACTGG GTGCGGGGGG CCCTTCTGGG CTGTGTGCCC
    CGGTGGCTTC TGATGAACCG GCCCCCACCA CCCGTGGAGC TCTGCCACCC CCTACGCCTG
    AAGCTCAGCC CCTCTTATCA CTGGCTGGAG AGCAACGTGG ATGCCGAGGA GAGGGAGGTG
    GTGGTGGAGG AGGAGGACAG ATGGGCATGT GCAGGTCATG TGGCCCCCTC TGTGGGCACC
    CTCTGCAGCC ACGGCCACCT GCACTCTGGG GCCTCAGGTC CCAAGGCTGA GGCTCTGCTG
    CAGGAGGGTG AGCTGCTGCT ATCACCCCAC ATGCAGAAGG CACTGGAAGG TGTGCACTAC
    ATTGCCGACC ACCTGCGGTC TGAGGATGCT GACTCTTCGG TGAAGGAGGA CTGGAAGTAT
    GTTGCCATGG TCATCGACAG GATCTTCCTC TGGCTGTTTA TCATCGTCTG CTTCCTGGGG
    ACCATCGGCC TCTTTCTGCC TCCGTTCCTA GCTGGAATGA TCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

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