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PPP1R21 cDNA ORF clone, Homo sapiens(human)

Summary

Gene Symbol PPP1R21
Entrez Gene ID 129285
Full Name protein phosphatase 1 regulatory subunit 21
Synonyms CCDC128,KLRAQ1
General protein information
Preferred Names

protein phosphatase 1 regulatory subunit 21

Names

protein phosphatase 1 regulatory subunit 21
KLRAQ motif containing 1
KLRAQ motif-containing protein 1
coiled-coil domain containing 128
coiled-coil domain-containing protein 128
Gene Type protein-coding
Organism Homo sapiens(human)
Genome

2

2p16.3

Reference Sequences (RefSeq)

mRNA and Protein(s)

mRNA Protein Name
XM_017003302.1 XP_016858791.1 protein phosphatase 1 regulatory subunit 21 isoform X2
NM_152994.4 NP_694539.1 protein phosphatase 1 regulatory subunit 21 isoform 2
NM_001193475.1 NP_001180404.1 protein phosphatase 1 regulatory subunit 21 isoform 5
XM_017003301.1 XP_016858790.1 protein phosphatase 1 regulatory subunit 21 isoform X1
NM_001135629.2 NP_001129101.1 protein phosphatase 1 regulatory subunit 21 isoform 1

Pathways from BioSystems


Homologies


Mus musculus (house mouse) Ppp1r21 NP_082934.3
Gallus gallus (chicken) PPP1R21 NP_001012891.1
Pan troglodytes (chimpanzee) KLRAQ1 XP_001151290.1
Canis lupus familiaris (dog) PPP1R21 XP_531815.2
Bos taurus (cattle) PPP1R21 NP_001096563.1
Rattus norvegicus (Norway rat) Ppp1r21 NP_001258234.1
Danio rerio (zebrafish) ppp1r21 NP_001007300.1
KLRAQ1 XP_001114046.1
Homo sapiens (human) PPP1R21 NP_001129101.1
Xenopus tropicalis (tropical clawed frog) ppp1r21 XP_002935419.2

Gene Ontology


Bibliography

Related articles in PubMed

Defining the membrane proteome of NK cells.
Ghosh D, Lippert D, Krokhin O, Cortens JP, Wilkins JA
Journal of mass spectrometry : JMS45(1)1-25(2010 Jan)

Docking motif-guided mapping of the interactome of protein phosphatase-1.
Hendrickx A, Beullens M, Ceulemans H, Den Abt T, Van Eynde A, Nicolaescu E, Lesage B, Bollen M
Chemistry & biology16(4)365-71(2009 Apr)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following PPP1R21 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the PPP1R21 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OHu55717 NM_001135629.2
Latest version!		 Homo sapiens protein phosphatase 1 regulatory subunit 21 (PPP1R21), transcript variant 1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK		 $189.50-$265.30
$379.00
OHu89000 XM_017003301.1
Latest version!		 Homo sapiens protein phosphatase 1 regulatory subunit 21 (PPP1R21), transcript variant X2, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 10-12 $307.30
$439.00
OHu05366 NM_001193475.1
Latest version!		 Homo sapiens protein phosphatase 1 regulatory subunit 21 (PPP1R21), transcript variant 5, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 12-14 $265.30
$379.00
OHu06956 NM_152994.4
Latest version!		 Homo sapiens protein phosphatase 1 regulatory subunit 21 (PPP1R21), transcript variant 2, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 12-14 $391.30
$559.00
OHu89001 XM_017003302.1
Latest version!		 Homo sapiens protein phosphatase 1 regulatory subunit 21 (PPP1R21), transcript variant X3, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 14-16 $307.30
$439.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)


CloneID OHu55717
Clone ID Related Accession (Same CDS sequence) NM_001135629.2
Accession Version NM_001135629.2 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 2343bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2017-10-02
Organism Homo sapiens(human)
Product protein phosphatase 1 regulatory subunit 21 isoform 1
Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AY134855.1, BC117279.1, AK172762.1 and AA449986.1. On Jul 28, 2010 this sequence version replaced NM_001135629.1. Transcript Variant: This variant (1) encodes the longest protein (isoform 1). ##Evidence-Data-START## Transcript exon combination :: AK172762.1, AY092062.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end. 

1
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1081
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1381
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1501
1561
1621
1681
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1801
1861
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1981
2041
2101
2161
2221
2281
2341
ATGGCCTCGG CTGAGTTGCA GGGGAAGTAC CAGAAGCTGG CTCAGGAGTA CTCGAAGCTT 
CGGGCTCAGA ATCAGGTTCT GAAAAAAGGT GTTGTGGATG AACAAGCAAA TTCTGCAGCT 
TTAAAGGAGC AACTGAAAAT GAAGGATCAG TCATTGAGAA AACTACAACA GGAAATGGAC 
AGTTTGACAT TTCGAAATCT GCAGCTTGCC AAGAGGGTAG AACTACTTCA AGATGAACTA 
GCTCTAAGTG AACCACGAGG CAAGAAAAAC AAGAAAAGTG GAGAATCTTC TTCTCAGTTG 
AGTCAAGAGC AGAAGAGTGT CTTTGATGAA GATCTGCAAA AGAAGATAGA AGAGAATGAA 
CGGTTGCATA TACAATTTTT TGAAGCTGAT GAGCAGCACA AGCATGTGGA AGCAGAGCTG 
AGGAGTCGAC TGGCCACTCT GGAGACAGAA GCAGCCCAGC ACCAAGCTGT GGTTGACGGT 
CTCACCCGGA AGTACATGGA AACCATTGAG AAGCTGCAGA ACGACAAGGC TAAACTAGAA 
GTGAAATCTC AGACTCTAGA AAAGGAAGCC AAGGAATGTC GACTTCGAAC GGAAGAATGT 
CAATTACAGT TAAAGACTCT TCATGAAGAT TTGTCAGGTA GATTAGAGGA ATCCTTATCA 
ATCATCAATG AAAAAGTACC TTTTAATGAT ACAAAATATA GTCAGTACAA CGCTCTGAAC 
GTTCCACTCC ACAATAGGAG ACACCAGCTG AAGATGCGAG ATATTGCTGG GCAGGCCCTG 
GCTTTTGTTC AGGATCTTGT GACGGCTCTT CTAAACTTTC ATACCTACAC AGAACAGAGG 
ATTCAAATTT TTCCTGTTGA TTCTGCCATT GACACTATAT CTCCATTGAA TCAGAAGTTC 
TCACAATACC TTCATGAAAA TGCGTCCTAT GTCCGCCCTC TTGAGGAAGG AATGCTTCAT 
TTATTTGAAA GTATCACTGA GGATACTGTG ACTGTCTTGG AGACAACTGT GAAATTGAAA 
ACTTTTTCAG AACACTTAAC CTCCTACATA TGTTTTCTTA GGAAGATTCT TCCCTATCAG 
TTAAAAAGTT TAGAAGAAGA ATGTGAATCC TCTCTTTGCA CATCTGCGTT AAGAGCCAGG 
AATCTAGAGC TGTCCCAGGA CATGAAAAAA ATGACAGCTG TGTTTGAGAA GCTGCAGACT 
TACATAGCTC TTCTTGCCTT GCCAAGTACA GAGCCAGATG GACTCCTTCG GACAAACTAC 
AGTTCTGTGT TAACAAATGT TGGTGCTGCT CTGCATGGAT TTCATGACGT TATGAAAGAT 
ATTTCCAAAC ATTATAGTCA AAAAGCTGCA ATAGAGCATG AACTTCCAAC AGCAACACAG 
AAGCTGATAA CAACTAATGA CTGTATCCTG TCATCAGTAG TGGCATTAAC AAATGGAGCA 
GGAAAGATTG CATCCTTCTT CAGCAACAAT TTGGACTACT TCATTGCTTC ACTGAGCTAT 
GGACCTAAGG CAGCGAGTGG ATTCATTAGT CCTCTTTCAG CTGAATGCAT GCTACAGTAT 
AAGAAAAAAG CTGCTGCCTA TATGAAGTCT TTGAGAAAGC CCCTCTTGGA GTCTGTGCCT 
TATGAAGAAG CACTGGCAAA CCGCCGCATC CTTCTCAGCT CTACTGAAAG TCGAGAAGGC 
CTTGCACAGC AAGTTCAACA GAGTTTGGAA AAGATTTCTA AACTGGAGCA GGAAAAAGAA 
CATTGGATGT TGGAAGCACA ATTAGCCAAA ATCAAGCTAG AGAAAGAAAA CCAGCGAATT 
GCAGATAAGC TGAAGAATAC AGGTAGTGCC CAGCTGGTTG GGCTGGCCCA GGAAAATGCT 
GCTGTGTCAA ATACTGCTGG CCAGGATGAA GCCACAGCTA AGGCTGTGTT GGAGCCCATT 
CAGAGCACCA GTCTAATTGG GACTTTAACC AGGACATCTG ACAGTGAGGT TCCAGATGTG 
GAATCTCGTG AAGACTTAAT TAAAAATCAC TACATGGCAA GGATAGTGGA ACTTACGTCT 
CAGTTGCAGC TGGCTGACAG TAAGTCAGTG CATTTTTATG CCGAGTGCCG AGCACTGTCT 
AAAAGACTGG CCTTGGCTGA AAAGTCTAAG GAAGCATTGA CAGAAGAAAT GAAACTTGCC 
AGTCAGAACA TCAGCAGACT TCAGGATGAG CTGACAACTA CCAAGAGGAG TTACGAGGAT 
CAGTTAAGTA TGATGAGTGA CCACCTGTGC AGCATGAATG AGACATTATC TAAACAGAGA 
GAAGAGATTG ACACACTAAA GATGTCCAGT AAGGGGAATT CTAAAAAGAA CAAGAGTCGA 
TAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001129101.1
CDS186..2528
Misc Feature(1)96..98
Misc Feature(2)<198..>1007
Misc Feature(3)216..518
Misc Feature(4)948..2498
Misc Feature(5)2139..2141
Exon (1)1..242
Exon (2)243..311
Exon (3)312..458
Exon (4)459..560
Exon (5)561..725
Exon (6)726..784
Exon (7)785..879
Exon (8)880..932
Exon (9)933..1082
Exon (10)1083..1184
Exon (11)1185..1273
Exon (12)1274..1410
Exon (13)1411..1503
Exon (14)1504..1631
Exon (15)1632..1784
Exon (16)1785..1877
Exon (17)1878..2120
Exon (18)2121..2153
Exon (19)2154..2270
Exon (20)2271..2369
Exon (21)2370..2498
Exon (22)2499..3176
Translation

Target ORF information:

RefSeq Version NM_001135629.2
Organism Homo sapiens(human)
Definition Homo sapiens protein phosphatase 1 regulatory subunit 21 (PPP1R21), transcript variant 1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 NM_001135629.2

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
ATGGCCTCGG CTGAGTTGCA GGGGAAGTAC CAGAAGCTGG CTCAGGAGTA CTCGAAGCTT 
CGGGCTCAGA ATCAGGTTCT GAAAAAAGGT GTTGTGGATG AACAAGCAAA TTCTGCAGCT 
TTAAAGGAGC AACTGAAAAT GAAGGATCAG TCATTGAGAA AACTACAACA GGAAATGGAC 
AGTTTGACAT TTCGAAATCT GCAGCTTGCC AAGAGGGTAG AACTACTTCA AGATGAACTA 
GCTCTAAGTG AACCACGAGG CAAGAAAAAC AAGAAAAGTG GAGAATCTTC TTCTCAGTTG 
AGTCAAGAGC AGAAGAGTGT CTTTGATGAA GATCTGCAAA AGAAGATAGA AGAGAATGAA 
CGGTTGCATA TACAATTTTT TGAAGCTGAT GAGCAGCACA AGCATGTGGA AGCAGAGCTG 
AGGAGTCGAC TGGCCACTCT GGAGACAGAA GCAGCCCAGC ACCAAGCTGT GGTTGACGGT 
CTCACCCGGA AGTACATGGA AACCATTGAG AAGCTGCAGA ACGACAAGGC TAAACTAGAA 
GTGAAATCTC AGACTCTAGA AAAGGAAGCC AAGGAATGTC GACTTCGAAC GGAAGAATGT 
CAATTACAGT TAAAGACTCT TCATGAAGAT TTGTCAGGTA GATTAGAGGA ATCCTTATCA 
ATCATCAATG AAAAAGTACC TTTTAATGAT ACAAAATATA GTCAGTACAA CGCTCTGAAC 
GTTCCACTCC ACAATAGGAG ACACCAGCTG AAGATGCGAG ATATTGCTGG GCAGGCCCTG 
GCTTTTGTTC AGGATCTTGT GACGGCTCTT CTAAACTTTC ATACCTACAC AGAACAGAGG 
ATTCAAATTT TTCCTGTTGA TTCTGCCATT GACACTATAT CTCCATTGAA TCAGAAGTTC 
TCACAATACC TTCATGAAAA TGCGTCCTAT GTCCGCCCTC TTGAGGAAGG AATGCTTCAT 
TTATTTGAAA GTATCACTGA GGATACTGTG ACTGTCTTGG AGACAACTGT GAAATTGAAA 
ACTTTTTCAG AACACTTAAC CTCCTACATA TGTTTTCTTA GGAAGATTCT TCCCTATCAG 
TTAAAAAGTT TAGAAGAAGA ATGTGAATCC TCTCTTTGCA CATCTGCGTT AAGAGCCAGG 
AATCTAGAGC TGTCCCAGGA CATGAAAAAA ATGACAGCTG TGTTTGAGAA GCTGCAGACT 
TACATAGCTC TTCTTGCCTT GCCAAGTACA GAGCCAGATG GACTCCTTCG GACAAACTAC 
AGTTCTGTGT TAACAAATGT TGGTGCTGCT CTGCATGGAT TTCATGACGT TATGAAAGAT 
ATTTCCAAAC ATTATAGTCA AAAAGCTGCA ATAGAGCATG AACTTCCAAC AGCAACACAG 
AAGCTGATAA CAACTAATGA CTGTATCCTG TCATCAGTAG TGGCATTAAC AAATGGAGCA 
GGAAAGATTG CATCCTTCTT CAGCAACAAT TTGGACTACT TCATTGCTTC ACTGAGCTAT 
GGACCTAAGG CAGCGAGTGG ATTCATTAGT CCTCTTTCAG CTGAATGCAT GCTACAGTAT 
AAGAAAAAAG CTGCTGCCTA TATGAAGTCT TTGAGAAAGC CCCTCTTGGA GTCTGTGCCT 
TATGAAGAAG CACTGGCAAA CCGCCGCATC CTTCTCAGCT CTACTGAAAG TCGAGAAGGC 
CTTGCACAGC AAGTTCAACA GAGTTTGGAA AAGATTTCTA AACTGGAGCA GGAAAAAGAA 
CATTGGATGT TGGAAGCACA ATTAGCCAAA ATCAAGCTAG AGAAAGAAAA CCAGCGAATT 
GCAGATAAGC TGAAGAATAC AGGTAGTGCC CAGCTGGTTG GGCTGGCCCA GGAAAATGCT 
GCTGTGTCAA ATACTGCTGG CCAGGATGAA GCCACAGCTA AGGCTGTGTT GGAGCCCATT 
CAGAGCACCA GTCTAATTGG GACTTTAACC AGGACATCTG ACAGTGAGGT TCCAGATGTG 
GAATCTCGTG AAGACTTAAT TAAAAATCAC TACATGGCAA GGATAGTGGA ACTTACGTCT 
CAGTTGCAGC TGGCTGACAG TAAGTCAGTG CATTTTTATG CCGAGTGCCG AGCACTGTCT 
AAAAGACTGG CCTTGGCTGA AAAGTCTAAG GAAGCATTGA CAGAAGAAAT GAAACTTGCC 
AGTCAGAACA TCAGCAGACT TCAGGATGAG CTGACAACTA CCAAGAGGAG TTACGAGGAT 
CAGTTAAGTA TGATGAGTGA CCACCTGTGC AGCATGAATG AGACATTATC TAAACAGAGA 
GAAGAGATTG ACACACTAAA GATGTCCAGT AAGGGGAATT CTAAAAAGAA CAAGAGTCGA 
TAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu89000
Clone ID Related Accession (Same CDS sequence) XM_017003301.1
Accession Version XM_017003301.1 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 1848bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2016-06-06
Organism Homo sapiens(human)
Product protein phosphatase 1 regulatory subunit 21 isoform X1
Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000002.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

1
61
121
181
241
301
361
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481
541
601
661
721
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841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
ATGGAAACCA TTGAGAAGCT GCAGAACGAC AAGGCTAAAC TAGAAGTGAA ATCTCAGACT 
CTAGAAAAGG AAGCCAAGGA ATGTCGACTT CGAACGGAAG AATGTCAATT ACAGTTAAAG 
ACTCTTCATG AAGATTTGTC AGGTAGATTA GAGGAATCCT TATCAATCAT CAATGAAAAA 
GTACCTTTTA ATGATACAAA ATATAGTCAG TACAACGCTC TGAACGTTCC ACTCCACAAT 
AGGAGACACC AGCTGAAGAT GCGAGATATT GCTGGGCAGG CCCTGGCTTT TGTTCAGGAT 
CTTGTGACGG CTCTTCTAAA CTTTCATACC TACACAGAAC AGAGGATTCA AATTTTTCCT 
GTTGATTCTG CCATTGACAC TATATCTCCA TTGAATCAGA AGTTCTCACA ATACCTTCAT 
GAAAATGCGT CCTATGTCCG CCCTCTTGAG GAAGGAATGC TTCATTTATT TGAAAGTATC 
ACTGAGGATA CTGTGACTGT CTTGGAGACA ACTGTGAAAT TGAAAACTTT TTCAGAACAC 
TTAACCTCCT ACATATGTTT TCTTAGGAAG ATTCTTCCCT ATCAGTTAAA AAGTTTAGAA 
GAAGAATGTG AATCCTCTCT TTGCACATCT GCGTTAAGAG CCAGGAATCT AGAGCTGTCC 
CAGGACATGA AAAAAATGAC AGCTGTGTTT GAGAAGCTGC AGACTTACAT AGCTCTTCTT 
GCCTTGCCAA GTACAGAGCC AGATGGACTC CTTCGGACAA ACTACAGTTC TGTGTTAACA 
AATGTTGGTG CTGCTCTGCA TGGATTTCAT GACGTTATGA AAGATATTTC CAAACATTAT 
AGTCAAAAAG CTGCAATAGA GCATGAACTT CCAACAGCAA CACAGAAGCT GATAACAACT 
AATGACTGTA TCCTGTCATC AGTAGTGGCA TTAACAAATG GAGCAGGAAA GATTGCATCC 
TTCTTCAGCA ACAATTTGGA CTACTTCATT GCTTCACTGA GCTATGGACC TAAGGCAGCG 
AGTGGATTCA TTAGTCCTCT TTCAGCTGAA TGCATGCTAC AGTATAAGAA AAAAGCTGCT 
GCCTATATGA AGTCTTTGAG AAAGCCCCTC TTGGAGTCTG TGCCTTATGA AGAAGCACTG 
GCAAACCGCC GCATCCTTCT CAGCTCTACT GAAAGTCGAG AAGGCCTTGC ACAGCAAGTT 
CAACAGAGTT TGGAAAAGAT TTCTAAACTG GAGCAGGAAA AAGAACATTG GATGTTGGAA 
GCACAATTAG CCAAAATCAA GCTAGAGAAA GAAAACCAGC GAATTGCAGA TAAGCTGAAG 
AATACAGGTA GTGCCCAGCT GGTTGGGCTG GCCCAGGAAA ATGCTGCTGT GTCAAATACT 
GCTGGCCAGG ATGAAGCCAC AGCTAAGGCT GTGTTGGAGC CCATTCAGAG CACCAGTCTA 
ATTGGGACTT TAACCAGGAC ATCTGACAGT GAGGTTCCAG ATGTGGAATC TCGTGAAGAC 
TTAATTAAAA ATCACTACAT GGCAAGGATA GTGGAACTTA CGTCTCAGTT GCAGCTGGCT 
GACAGTAAGT CAGTGCATTT TTATGCCGAG TGCCGAGCAC TGTCTAAAAG ACTGGCCTTG 
GCTGAAAAGT CTAAGGAAGC ATTGACAGAA GAAATGAAAC TTGCCAGTCA GAACATCAGC 
AGACTTCAGG ATGAGCTGAC AACTACCAAG AGGAGTTACG AGGATCAGTT AAGTATGATG 
AGTGACCACC TGTGCAGCAT GAATGAGACA TTATCTAAAC AGAGAGAAGA GATTGACACA 
CTAAAGATGT CCAGTAAGGG GAATTCTAAA AAGAACAAGA GTCGATAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_016858790.1
CDS716..2563
Translation

Target ORF information:

RefSeq Version XM_017003301.1
Organism Homo sapiens(human)
Definition Homo sapiens protein phosphatase 1 regulatory subunit 21 (PPP1R21), transcript variant X2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 XM_017003301.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
ATGGAAACCA TTGAGAAGCT GCAGAACGAC AAGGCTAAAC TAGAAGTGAA ATCTCAGACT 
CTAGAAAAGG AAGCCAAGGA ATGTCGACTT CGAACGGAAG AATGTCAATT ACAGTTAAAG 
ACTCTTCATG AAGATTTGTC AGGTAGATTA GAGGAATCCT TATCAATCAT CAATGAAAAA 
GTACCTTTTA ATGATACAAA ATATAGTCAG TACAACGCTC TGAACGTTCC ACTCCACAAT 
AGGAGACACC AGCTGAAGAT GCGAGATATT GCTGGGCAGG CCCTGGCTTT TGTTCAGGAT 
CTTGTGACGG CTCTTCTAAA CTTTCATACC TACACAGAAC AGAGGATTCA AATTTTTCCT 
GTTGATTCTG CCATTGACAC TATATCTCCA TTGAATCAGA AGTTCTCACA ATACCTTCAT 
GAAAATGCGT CCTATGTCCG CCCTCTTGAG GAAGGAATGC TTCATTTATT TGAAAGTATC 
ACTGAGGATA CTGTGACTGT CTTGGAGACA ACTGTGAAAT TGAAAACTTT TTCAGAACAC 
TTAACCTCCT ACATATGTTT TCTTAGGAAG ATTCTTCCCT ATCAGTTAAA AAGTTTAGAA 
GAAGAATGTG AATCCTCTCT TTGCACATCT GCGTTAAGAG CCAGGAATCT AGAGCTGTCC 
CAGGACATGA AAAAAATGAC AGCTGTGTTT GAGAAGCTGC AGACTTACAT AGCTCTTCTT 
GCCTTGCCAA GTACAGAGCC AGATGGACTC CTTCGGACAA ACTACAGTTC TGTGTTAACA 
AATGTTGGTG CTGCTCTGCA TGGATTTCAT GACGTTATGA AAGATATTTC CAAACATTAT 
AGTCAAAAAG CTGCAATAGA GCATGAACTT CCAACAGCAA CACAGAAGCT GATAACAACT 
AATGACTGTA TCCTGTCATC AGTAGTGGCA TTAACAAATG GAGCAGGAAA GATTGCATCC 
TTCTTCAGCA ACAATTTGGA CTACTTCATT GCTTCACTGA GCTATGGACC TAAGGCAGCG 
AGTGGATTCA TTAGTCCTCT TTCAGCTGAA TGCATGCTAC AGTATAAGAA AAAAGCTGCT 
GCCTATATGA AGTCTTTGAG AAAGCCCCTC TTGGAGTCTG TGCCTTATGA AGAAGCACTG 
GCAAACCGCC GCATCCTTCT CAGCTCTACT GAAAGTCGAG AAGGCCTTGC ACAGCAAGTT 
CAACAGAGTT TGGAAAAGAT TTCTAAACTG GAGCAGGAAA AAGAACATTG GATGTTGGAA 
GCACAATTAG CCAAAATCAA GCTAGAGAAA GAAAACCAGC GAATTGCAGA TAAGCTGAAG 
AATACAGGTA GTGCCCAGCT GGTTGGGCTG GCCCAGGAAA ATGCTGCTGT GTCAAATACT 
GCTGGCCAGG ATGAAGCCAC AGCTAAGGCT GTGTTGGAGC CCATTCAGAG CACCAGTCTA 
ATTGGGACTT TAACCAGGAC ATCTGACAGT GAGGTTCCAG ATGTGGAATC TCGTGAAGAC 
TTAATTAAAA ATCACTACAT GGCAAGGATA GTGGAACTTA CGTCTCAGTT GCAGCTGGCT 
GACAGTAAGT CAGTGCATTT TTATGCCGAG TGCCGAGCAC TGTCTAAAAG ACTGGCCTTG 
GCTGAAAAGT CTAAGGAAGC ATTGACAGAA GAAATGAAAC TTGCCAGTCA GAACATCAGC 
AGACTTCAGG ATGAGCTGAC AACTACCAAG AGGAGTTACG AGGATCAGTT AAGTATGATG 
AGTGACCACC TGTGCAGCAT GAATGAGACA TTATCTAAAC AGAGAGAAGA GATTGACACA 
CTAAAGATGT CCAGTAAGGG GAATTCTAAA AAGAACAAGA GTCGATAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu05366
Clone ID Related Accession (Same CDS sequence) NM_001193475.1
Accession Version NM_001193475.1 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 2217bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2017-10-02
Organism Homo sapiens(human)
Product protein phosphatase 1 regulatory subunit 21 isoform 5
Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AY134855.1, BC143466.1, AK172762.1 and AA449986.1. Transcript Variant: This variant (5) lacks two alternate in-frame exons in the coding region, compared to variant 1. The resulting protein (isoform 5) is shorter than isoform 1. ##Evidence-Data-START## Transcript exon combination :: BC143466.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968540, SAMEA1968968 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end. 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
ATGGCCTCGG CTGAGTTGCA GGGGAAGTAC CAGAAGCTGG CTCAGGAGTA CTCGAAGCTT 
CGGGCTCAGA ATCAGGTTCT GAAAAAAGGT GTTGTGGATG AACAAGCAAA TTCTGCAGCT 
TTAAAGGAGC AACTGAAAAT GAAGGATCAG TCATTGAGAA AACTACAACA GGAAATGGAC 
AGTTTGACAT TTCGAAATCT GCAGCTTGCC AAGAGGGTAG AACTACTTCA AGATGAACTA 
GCTCTAAGTG AACCACGAGG CAAGAAAAAC AAGAAAAGTG GAGAATCTTC TTCTCAGTTG 
AGTCAAGAGC AGAAGAGTGT CTTTGATGAA GATCTGCAAA AGAAGATAGA AGAGAATGAA 
CGGTTGCATA TACAATTTTT TGAAGCTGAT GAGCAGCACA AGCATGTGGA AGCAGAGCTG 
AGGAGTCGAC TGGCCACTCT GGAGACAGAA GCAGCCCAGC ACCAAGCTGT GGTTGACGGT 
CTCACCCGGA AGTACATGGA AACCATTGAG AAGCTGCAGA ACGACAAGGC TAAACTAGAA 
GTGAAATCTC AGACTCTAGA AAAGGAAGCC AAGGAATGTC GACTTCGAAC GGAAGAATGT 
CAATTACAGT TAAAGACTCT TCATGAAGAT TTGTCAGGTA GATTAGAGGA ATCCTTATCA 
ATCATCAATG AAAAAGTACC TTTTAATGAT ACAAAATATA GTCAGTACAA CGCTCTGAAC 
GTTCCACTCC ACAATAGGAG ACACCAGCTG AAGATGCGAG ATATTGCTGG GCAGGCCCTG 
GCTTTTGTTC AGGATCTTGT GACGGCTCTT CTAAACTTTC ATACCTACAC AGAACAGAGG 
ATTCAAATTT TTCCTGTTGA TTCTGCCATT GACACTATAT CTCCATTGAA TCAGAAGTTC 
TCACAATACC TTCATGAAAA TGCGTCCTAT GTCCGCCCTC TTGAGGAAGG AATGCTTCAT 
TTATTTGAAA GTATCACTGA GGATACTGTG ACTGTCTTGG AGACAACTGT GAAATTGAAA 
ACTTTTTCAG AACACTTAAC CTCCTACATA TGTTTTCTTA GGAAGATTCT TCCCTATCAG 
TTAAAAAGTT TAGAAGAAGA ATGTGAATCC TCTCTTTGCA CATCTGCGTT AAGAGCCAGG 
AATCTAGAGC TGTCCCAGGA CATGAAAAAA ATGACAGCTG TGTTTGAGAA GCTGCAGACT 
TACATAGCTC TTCTTGCCTT GCCAAGTACA GAGCCAGATG GACTCCTTCG GACAAACTAC 
AGTTCTGTGT TAACAAATGT TGGTGCTGCT CTGCATGGAT TTCATGACGT TATGAAAGAT 
ATTTCCAAAC ATTATAGTCA AAAAGCTGCA ATAGAGCATG AACTTCCAAC AGCAACACAG 
AAGCTGATAA CAACTAATGA CTGTATCCTG TCATCAGTAG TGGCATTAAC AAATGGAGCA 
GGAAAGATTG CATCCTTCTT CAGCAACAAT TTGGACTACT TCATTGCTTC ACTGAGCTAT 
GGACCTAAGG CAGCGAGTGG ATTCATTAGT CCTCTTTCAG CTGAATGCAT GCTACAGTAT 
AAGAAAAAAG CTGCTGCCTA TATGAAGTCT TTGAGAAAGG TTCAACAGAG TTTGGAAAAG 
ATTTCTAAAC TGGAGCAGGA AAAAGAACAT TGGATGTTGG AAGCACAATT AGCCAAAATC 
AAGCTAGAGA AAGAAAACCA GCGAATTGCA GATAAGCTGA AGAATACAGG TAGTGCCCAG 
CTGGTTGGGC TGGCCCAGGA AAATGCTGCT GTGTCAAATA CTGCTGGCCA GGATGAAGCC 
ACAGCTAAGG CTGTGTTGGA GCCCATTCAG AGCACCAGTC TAGTTCCAGA TGTGGAATCT 
CGTGAAGACT TAATTAAAAA TCACTACATG GCAAGGATAG TGGAACTTAC GTCTCAGTTG 
CAGCTGGCTG ACAGTAAGTC AGTGCATTTT TATGCCGAGT GCCGAGCACT GTCTAAAAGA 
CTGGCCTTGG CTGAAAAGTC TAAGGAAGCA TTGACAGAAG AAATGAAACT TGCCAGTCAG 
AACATCAGCA GACTTCAGGA TGAGCTGACA ACTACCAAGA GGAGTTACGA GGATCAGTTA 
AGTATGATGA GTGACCACCT GTGCAGCATG AATGAGACAT TATCTAAACA GAGAGAAGAG 
ATTGACACAC TAAAGATGTC CAGTAAGGGG AATTCTAAAA AGAACAAGAG TCGATAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001180404.1
CDS186..2402
Misc Feature(1)96..98
Misc Feature(2)216..518
Misc Feature(3)948..2372
Exon (1)1..242
Exon (2)243..311
Exon (3)312..458
Exon (4)459..560
Exon (5)561..725
Exon (6)726..784
Exon (7)785..879
Exon (8)880..932
Exon (9)933..1082
Exon (10)1083..1184
Exon (11)1185..1273
Exon (12)1274..1410
Exon (13)1411..1503
Exon (14)1504..1631
Exon (15)1632..1784
Exon (16)1785..2027
Exon (17)2028..2144
Exon (18)2145..2243
Exon (19)2244..2372
Exon (20)2373..3050
Translation

Target ORF information:

RefSeq Version NM_001193475.1
Organism Homo sapiens(human)
Definition Homo sapiens protein phosphatase 1 regulatory subunit 21 (PPP1R21), transcript variant 5, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 NM_001193475.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
ATGGCCTCGG CTGAGTTGCA GGGGAAGTAC CAGAAGCTGG CTCAGGAGTA CTCGAAGCTT 
CGGGCTCAGA ATCAGGTTCT GAAAAAAGGT GTTGTGGATG AACAAGCAAA TTCTGCAGCT 
TTAAAGGAGC AACTGAAAAT GAAGGATCAG TCATTGAGAA AACTACAACA GGAAATGGAC 
AGTTTGACAT TTCGAAATCT GCAGCTTGCC AAGAGGGTAG AACTACTTCA AGATGAACTA 
GCTCTAAGTG AACCACGAGG CAAGAAAAAC AAGAAAAGTG GAGAATCTTC TTCTCAGTTG 
AGTCAAGAGC AGAAGAGTGT CTTTGATGAA GATCTGCAAA AGAAGATAGA AGAGAATGAA 
CGGTTGCATA TACAATTTTT TGAAGCTGAT GAGCAGCACA AGCATGTGGA AGCAGAGCTG 
AGGAGTCGAC TGGCCACTCT GGAGACAGAA GCAGCCCAGC ACCAAGCTGT GGTTGACGGT 
CTCACCCGGA AGTACATGGA AACCATTGAG AAGCTGCAGA ACGACAAGGC TAAACTAGAA 
GTGAAATCTC AGACTCTAGA AAAGGAAGCC AAGGAATGTC GACTTCGAAC GGAAGAATGT 
CAATTACAGT TAAAGACTCT TCATGAAGAT TTGTCAGGTA GATTAGAGGA ATCCTTATCA 
ATCATCAATG AAAAAGTACC TTTTAATGAT ACAAAATATA GTCAGTACAA CGCTCTGAAC 
GTTCCACTCC ACAATAGGAG ACACCAGCTG AAGATGCGAG ATATTGCTGG GCAGGCCCTG 
GCTTTTGTTC AGGATCTTGT GACGGCTCTT CTAAACTTTC ATACCTACAC AGAACAGAGG 
ATTCAAATTT TTCCTGTTGA TTCTGCCATT GACACTATAT CTCCATTGAA TCAGAAGTTC 
TCACAATACC TTCATGAAAA TGCGTCCTAT GTCCGCCCTC TTGAGGAAGG AATGCTTCAT 
TTATTTGAAA GTATCACTGA GGATACTGTG ACTGTCTTGG AGACAACTGT GAAATTGAAA 
ACTTTTTCAG AACACTTAAC CTCCTACATA TGTTTTCTTA GGAAGATTCT TCCCTATCAG 
TTAAAAAGTT TAGAAGAAGA ATGTGAATCC TCTCTTTGCA CATCTGCGTT AAGAGCCAGG 
AATCTAGAGC TGTCCCAGGA CATGAAAAAA ATGACAGCTG TGTTTGAGAA GCTGCAGACT 
TACATAGCTC TTCTTGCCTT GCCAAGTACA GAGCCAGATG GACTCCTTCG GACAAACTAC 
AGTTCTGTGT TAACAAATGT TGGTGCTGCT CTGCATGGAT TTCATGACGT TATGAAAGAT 
ATTTCCAAAC ATTATAGTCA AAAAGCTGCA ATAGAGCATG AACTTCCAAC AGCAACACAG 
AAGCTGATAA CAACTAATGA CTGTATCCTG TCATCAGTAG TGGCATTAAC AAATGGAGCA 
GGAAAGATTG CATCCTTCTT CAGCAACAAT TTGGACTACT TCATTGCTTC ACTGAGCTAT 
GGACCTAAGG CAGCGAGTGG ATTCATTAGT CCTCTTTCAG CTGAATGCAT GCTACAGTAT 
AAGAAAAAAG CTGCTGCCTA TATGAAGTCT TTGAGAAAGG TTCAACAGAG TTTGGAAAAG 
ATTTCTAAAC TGGAGCAGGA AAAAGAACAT TGGATGTTGG AAGCACAATT AGCCAAAATC 
AAGCTAGAGA AAGAAAACCA GCGAATTGCA GATAAGCTGA AGAATACAGG TAGTGCCCAG 
CTGGTTGGGC TGGCCCAGGA AAATGCTGCT GTGTCAAATA CTGCTGGCCA GGATGAAGCC 
ACAGCTAAGG CTGTGTTGGA GCCCATTCAG AGCACCAGTC TAGTTCCAGA TGTGGAATCT 
CGTGAAGACT TAATTAAAAA TCACTACATG GCAAGGATAG TGGAACTTAC GTCTCAGTTG 
CAGCTGGCTG ACAGTAAGTC AGTGCATTTT TATGCCGAGT GCCGAGCACT GTCTAAAAGA 
CTGGCCTTGG CTGAAAAGTC TAAGGAAGCA TTGACAGAAG AAATGAAACT TGCCAGTCAG 
AACATCAGCA GACTTCAGGA TGAGCTGACA ACTACCAAGA GGAGTTACGA GGATCAGTTA 
AGTATGATGA GTGACCACCT GTGCAGCATG AATGAGACAT TATCTAAACA GAGAGAAGAG 
ATTGACACAC TAAAGATGTC CAGTAAGGGG AATTCTAAAA AGAACAAGAG TCGATAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu06956
Clone ID Related Accession (Same CDS sequence) NM_152994.4
Accession Version NM_152994.4 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 2310bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2017-10-02
Organism Homo sapiens(human)
Product protein phosphatase 1 regulatory subunit 21 isoform 2
Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AY134855.1, AK172762.1 and AA449986.1. On Jul 28, 2010 this sequence version replaced NM_152994.3. Transcript Variant: This variant (2) lacks an alternate in-frame exon in the 3' coding region, compared to variant 1. The resulting protein (isoform 2) is shorter than isoform 1. ##Evidence-Data-START## Transcript exon combination :: AY134855.1, SRR1803613.142894.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end. 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
ATGGCCTCGG CTGAGTTGCA GGGGAAGTAC CAGAAGCTGG CTCAGGAGTA CTCGAAGCTT 
CGGGCTCAGA ATCAGGTTCT GAAAAAAGGT GTTGTGGATG AACAAGCAAA TTCTGCAGCT 
TTAAAGGAGC AACTGAAAAT GAAGGATCAG TCATTGAGAA AACTACAACA GGAAATGGAC 
AGTTTGACAT TTCGAAATCT GCAGCTTGCC AAGAGGGTAG AACTACTTCA AGATGAACTA 
GCTCTAAGTG AACCACGAGG CAAGAAAAAC AAGAAAAGTG GAGAATCTTC TTCTCAGTTG 
AGTCAAGAGC AGAAGAGTGT CTTTGATGAA GATCTGCAAA AGAAGATAGA AGAGAATGAA 
CGGTTGCATA TACAATTTTT TGAAGCTGAT GAGCAGCACA AGCATGTGGA AGCAGAGCTG 
AGGAGTCGAC TGGCCACTCT GGAGACAGAA GCAGCCCAGC ACCAAGCTGT GGTTGACGGT 
CTCACCCGGA AGTACATGGA AACCATTGAG AAGCTGCAGA ACGACAAGGC TAAACTAGAA 
GTGAAATCTC AGACTCTAGA AAAGGAAGCC AAGGAATGTC GACTTCGAAC GGAAGAATGT 
CAATTACAGT TAAAGACTCT TCATGAAGAT TTGTCAGGTA GATTAGAGGA ATCCTTATCA 
ATCATCAATG AAAAAGTACC TTTTAATGAT ACAAAATATA GTCAGTACAA CGCTCTGAAC 
GTTCCACTCC ACAATAGGAG ACACCAGCTG AAGATGCGAG ATATTGCTGG GCAGGCCCTG 
GCTTTTGTTC AGGATCTTGT GACGGCTCTT CTAAACTTTC ATACCTACAC AGAACAGAGG 
ATTCAAATTT TTCCTGTTGA TTCTGCCATT GACACTATAT CTCCATTGAA TCAGAAGTTC 
TCACAATACC TTCATGAAAA TGCGTCCTAT GTCCGCCCTC TTGAGGAAGG AATGCTTCAT 
TTATTTGAAA GTATCACTGA GGATACTGTG ACTGTCTTGG AGACAACTGT GAAATTGAAA 
ACTTTTTCAG AACACTTAAC CTCCTACATA TGTTTTCTTA GGAAGATTCT TCCCTATCAG 
TTAAAAAGTT TAGAAGAAGA ATGTGAATCC TCTCTTTGCA CATCTGCGTT AAGAGCCAGG 
AATCTAGAGC TGTCCCAGGA CATGAAAAAA ATGACAGCTG TGTTTGAGAA GCTGCAGACT 
TACATAGCTC TTCTTGCCTT GCCAAGTACA GAGCCAGATG GACTCCTTCG GACAAACTAC 
AGTTCTGTGT TAACAAATGT TGGTGCTGCT CTGCATGGAT TTCATGACGT TATGAAAGAT 
ATTTCCAAAC ATTATAGTCA AAAAGCTGCA ATAGAGCATG AACTTCCAAC AGCAACACAG 
AAGCTGATAA CAACTAATGA CTGTATCCTG TCATCAGTAG TGGCATTAAC AAATGGAGCA 
GGAAAGATTG CATCCTTCTT CAGCAACAAT TTGGACTACT TCATTGCTTC ACTGAGCTAT 
GGACCTAAGG CAGCGAGTGG ATTCATTAGT CCTCTTTCAG CTGAATGCAT GCTACAGTAT 
AAGAAAAAAG CTGCTGCCTA TATGAAGTCT TTGAGAAAGC CCCTCTTGGA GTCTGTGCCT 
TATGAAGAAG CACTGGCAAA CCGCCGCATC CTTCTCAGCT CTACTGAAAG TCGAGAAGGC 
CTTGCACAGC AAGTTCAACA GAGTTTGGAA AAGATTTCTA AACTGGAGCA GGAAAAAGAA 
CATTGGATGT TGGAAGCACA ATTAGCCAAA ATCAAGCTAG AGAAAGAAAA CCAGCGAATT 
GCAGATAAGC TGAAGAATAC AGGTAGTGCC CAGCTGGTTG GGCTGGCCCA GGAAAATGCT 
GCTGTGTCAA ATACTGCTGG CCAGGATGAA GCCACAGCTA AGGCTGTGTT GGAGCCCATT 
CAGAGCACCA GTCTAGTTCC AGATGTGGAA TCTCGTGAAG ACTTAATTAA AAATCACTAC 
ATGGCAAGGA TAGTGGAACT TACGTCTCAG TTGCAGCTGG CTGACAGTAA GTCAGTGCAT 
TTTTATGCCG AGTGCCGAGC ACTGTCTAAA AGACTGGCCT TGGCTGAAAA GTCTAAGGAA 
GCATTGACAG AAGAAATGAA ACTTGCCAGT CAGAACATCA GCAGACTTCA GGATGAGCTG 
ACAACTACCA AGAGGAGTTA CGAGGATCAG TTAAGTATGA TGAGTGACCA CCTGTGCAGC 
ATGAATGAGA CATTATCTAA ACAGAGAGAA GAGATTGACA CACTAAAGAT GTCCAGTAAG 
GGGAATTCTA AAAAGAACAA GAGTCGATAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_694539.1
CDS186..2495
Misc Feature(1)96..98
Misc Feature(2)216..518
Misc Feature(3)948..2465
Exon (1)1..242
Exon (2)243..311
Exon (3)312..458
Exon (4)459..560
Exon (5)561..725
Exon (6)726..784
Exon (7)785..879
Exon (8)880..932
Exon (9)933..1082
Exon (10)1083..1184
Exon (11)1185..1273
Exon (12)1274..1410
Exon (13)1411..1503
Exon (14)1504..1631
Exon (15)1632..1784
Exon (16)1785..1877
Exon (17)1878..2120
Exon (18)2121..2237
Exon (19)2238..2336
Exon (20)2337..2465
Exon (21)2466..3143
Translation

Target ORF information:

RefSeq Version NM_152994.4
Organism Homo sapiens(human)
Definition Homo sapiens protein phosphatase 1 regulatory subunit 21 (PPP1R21), transcript variant 2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 NM_152994.4

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
ATGGCCTCGG CTGAGTTGCA GGGGAAGTAC CAGAAGCTGG CTCAGGAGTA CTCGAAGCTT 
CGGGCTCAGA ATCAGGTTCT GAAAAAAGGT GTTGTGGATG AACAAGCAAA TTCTGCAGCT 
TTAAAGGAGC AACTGAAAAT GAAGGATCAG TCATTGAGAA AACTACAACA GGAAATGGAC 
AGTTTGACAT TTCGAAATCT GCAGCTTGCC AAGAGGGTAG AACTACTTCA AGATGAACTA 
GCTCTAAGTG AACCACGAGG CAAGAAAAAC AAGAAAAGTG GAGAATCTTC TTCTCAGTTG 
AGTCAAGAGC AGAAGAGTGT CTTTGATGAA GATCTGCAAA AGAAGATAGA AGAGAATGAA 
CGGTTGCATA TACAATTTTT TGAAGCTGAT GAGCAGCACA AGCATGTGGA AGCAGAGCTG 
AGGAGTCGAC TGGCCACTCT GGAGACAGAA GCAGCCCAGC ACCAAGCTGT GGTTGACGGT 
CTCACCCGGA AGTACATGGA AACCATTGAG AAGCTGCAGA ACGACAAGGC TAAACTAGAA 
GTGAAATCTC AGACTCTAGA AAAGGAAGCC AAGGAATGTC GACTTCGAAC GGAAGAATGT 
CAATTACAGT TAAAGACTCT TCATGAAGAT TTGTCAGGTA GATTAGAGGA ATCCTTATCA 
ATCATCAATG AAAAAGTACC TTTTAATGAT ACAAAATATA GTCAGTACAA CGCTCTGAAC 
GTTCCACTCC ACAATAGGAG ACACCAGCTG AAGATGCGAG ATATTGCTGG GCAGGCCCTG 
GCTTTTGTTC AGGATCTTGT GACGGCTCTT CTAAACTTTC ATACCTACAC AGAACAGAGG 
ATTCAAATTT TTCCTGTTGA TTCTGCCATT GACACTATAT CTCCATTGAA TCAGAAGTTC 
TCACAATACC TTCATGAAAA TGCGTCCTAT GTCCGCCCTC TTGAGGAAGG AATGCTTCAT 
TTATTTGAAA GTATCACTGA GGATACTGTG ACTGTCTTGG AGACAACTGT GAAATTGAAA 
ACTTTTTCAG AACACTTAAC CTCCTACATA TGTTTTCTTA GGAAGATTCT TCCCTATCAG 
TTAAAAAGTT TAGAAGAAGA ATGTGAATCC TCTCTTTGCA CATCTGCGTT AAGAGCCAGG 
AATCTAGAGC TGTCCCAGGA CATGAAAAAA ATGACAGCTG TGTTTGAGAA GCTGCAGACT 
TACATAGCTC TTCTTGCCTT GCCAAGTACA GAGCCAGATG GACTCCTTCG GACAAACTAC 
AGTTCTGTGT TAACAAATGT TGGTGCTGCT CTGCATGGAT TTCATGACGT TATGAAAGAT 
ATTTCCAAAC ATTATAGTCA AAAAGCTGCA ATAGAGCATG AACTTCCAAC AGCAACACAG 
AAGCTGATAA CAACTAATGA CTGTATCCTG TCATCAGTAG TGGCATTAAC AAATGGAGCA 
GGAAAGATTG CATCCTTCTT CAGCAACAAT TTGGACTACT TCATTGCTTC ACTGAGCTAT 
GGACCTAAGG CAGCGAGTGG ATTCATTAGT CCTCTTTCAG CTGAATGCAT GCTACAGTAT 
AAGAAAAAAG CTGCTGCCTA TATGAAGTCT TTGAGAAAGC CCCTCTTGGA GTCTGTGCCT 
TATGAAGAAG CACTGGCAAA CCGCCGCATC CTTCTCAGCT CTACTGAAAG TCGAGAAGGC 
CTTGCACAGC AAGTTCAACA GAGTTTGGAA AAGATTTCTA AACTGGAGCA GGAAAAAGAA 
CATTGGATGT TGGAAGCACA ATTAGCCAAA ATCAAGCTAG AGAAAGAAAA CCAGCGAATT 
GCAGATAAGC TGAAGAATAC AGGTAGTGCC CAGCTGGTTG GGCTGGCCCA GGAAAATGCT 
GCTGTGTCAA ATACTGCTGG CCAGGATGAA GCCACAGCTA AGGCTGTGTT GGAGCCCATT 
CAGAGCACCA GTCTAGTTCC AGATGTGGAA TCTCGTGAAG ACTTAATTAA AAATCACTAC 
ATGGCAAGGA TAGTGGAACT TACGTCTCAG TTGCAGCTGG CTGACAGTAA GTCAGTGCAT 
TTTTATGCCG AGTGCCGAGC ACTGTCTAAA AGACTGGCCT TGGCTGAAAA GTCTAAGGAA 
GCATTGACAG AAGAAATGAA ACTTGCCAGT CAGAACATCA GCAGACTTCA GGATGAGCTG 
ACAACTACCA AGAGGAGTTA CGAGGATCAG TTAAGTATGA TGAGTGACCA CCTGTGCAGC 
ATGAATGAGA CATTATCTAA ACAGAGAGAA GAGATTGACA CACTAAAGAT GTCCAGTAAG 
GGGAATTCTA AAAAGAACAA GAGTCGATAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu89001
Clone ID Related Accession (Same CDS sequence) XM_017003302.1
Accession Version XM_017003302.1 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 1815bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2016-06-06
Organism Homo sapiens(human)
Product protein phosphatase 1 regulatory subunit 21 isoform X2
Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000002.12) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
ATGGAAACCA TTGAGAAGCT GCAGAACGAC AAGGCTAAAC TAGAAGTGAA ATCTCAGACT 
CTAGAAAAGG AAGCCAAGGA ATGTCGACTT CGAACGGAAG AATGTCAATT ACAGTTAAAG 
ACTCTTCATG AAGATTTGTC AGGTAGATTA GAGGAATCCT TATCAATCAT CAATGAAAAA 
GTACCTTTTA ATGATACAAA ATATAGTCAG TACAACGCTC TGAACGTTCC ACTCCACAAT 
AGGAGACACC AGCTGAAGAT GCGAGATATT GCTGGGCAGG CCCTGGCTTT TGTTCAGGAT 
CTTGTGACGG CTCTTCTAAA CTTTCATACC TACACAGAAC AGAGGATTCA AATTTTTCCT 
GTTGATTCTG CCATTGACAC TATATCTCCA TTGAATCAGA AGTTCTCACA ATACCTTCAT 
GAAAATGCGT CCTATGTCCG CCCTCTTGAG GAAGGAATGC TTCATTTATT TGAAAGTATC 
ACTGAGGATA CTGTGACTGT CTTGGAGACA ACTGTGAAAT TGAAAACTTT TTCAGAACAC 
TTAACCTCCT ACATATGTTT TCTTAGGAAG ATTCTTCCCT ATCAGTTAAA AAGTTTAGAA 
GAAGAATGTG AATCCTCTCT TTGCACATCT GCGTTAAGAG CCAGGAATCT AGAGCTGTCC 
CAGGACATGA AAAAAATGAC AGCTGTGTTT GAGAAGCTGC AGACTTACAT AGCTCTTCTT 
GCCTTGCCAA GTACAGAGCC AGATGGACTC CTTCGGACAA ACTACAGTTC TGTGTTAACA 
AATGTTGGTG CTGCTCTGCA TGGATTTCAT GACGTTATGA AAGATATTTC CAAACATTAT 
AGTCAAAAAG CTGCAATAGA GCATGAACTT CCAACAGCAA CACAGAAGCT GATAACAACT 
AATGACTGTA TCCTGTCATC AGTAGTGGCA TTAACAAATG GAGCAGGAAA GATTGCATCC 
TTCTTCAGCA ACAATTTGGA CTACTTCATT GCTTCACTGA GCTATGGACC TAAGGCAGCG 
AGTGGATTCA TTAGTCCTCT TTCAGCTGAA TGCATGCTAC AGTATAAGAA AAAAGCTGCT 
GCCTATATGA AGTCTTTGAG AAAGCCCCTC TTGGAGTCTG TGCCTTATGA AGAAGCACTG 
GCAAACCGCC GCATCCTTCT CAGCTCTACT GAAAGTCGAG AAGGCCTTGC ACAGCAAGTT 
CAACAGAGTT TGGAAAAGAT TTCTAAACTG GAGCAGGAAA AAGAACATTG GATGTTGGAA 
GCACAATTAG CCAAAATCAA GCTAGAGAAA GAAAACCAGC GAATTGCAGA TAAGCTGAAG 
AATACAGGTA GTGCCCAGCT GGTTGGGCTG GCCCAGGAAA ATGCTGCTGT GTCAAATACT 
GCTGGCCAGG ATGAAGCCAC AGCTAAGGCT GTGTTGGAGC CCATTCAGAG CACCAGTCTA 
GTTCCAGATG TGGAATCTCG TGAAGACTTA ATTAAAAATC ACTACATGGC AAGGATAGTG 
GAACTTACGT CTCAGTTGCA GCTGGCTGAC AGTAAGTCAG TGCATTTTTA TGCCGAGTGC 
CGAGCACTGT CTAAAAGACT GGCCTTGGCT GAAAAGTCTA AGGAAGCATT GACAGAAGAA 
ATGAAACTTG CCAGTCAGAA CATCAGCAGA CTTCAGGATG AGCTGACAAC TACCAAGAGG 
AGTTACGAGG ATCAGTTAAG TATGATGAGT GACCACCTGT GCAGCATGAA TGAGACATTA 
TCTAAACAGA GAGAAGAGAT TGACACACTA AAGATGTCCA GTAAGGGGAA TTCTAAAAAG 
AACAAGAGTC GATAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_016858791.1
CDS716..2530
Translation

Target ORF information:

RefSeq Version XM_017003302.1
Organism Homo sapiens(human)
Definition Homo sapiens protein phosphatase 1 regulatory subunit 21 (PPP1R21), transcript variant X3, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 XM_017003302.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
ATGGAAACCA TTGAGAAGCT GCAGAACGAC AAGGCTAAAC TAGAAGTGAA ATCTCAGACT 
CTAGAAAAGG AAGCCAAGGA ATGTCGACTT CGAACGGAAG AATGTCAATT ACAGTTAAAG 
ACTCTTCATG AAGATTTGTC AGGTAGATTA GAGGAATCCT TATCAATCAT CAATGAAAAA 
GTACCTTTTA ATGATACAAA ATATAGTCAG TACAACGCTC TGAACGTTCC ACTCCACAAT 
AGGAGACACC AGCTGAAGAT GCGAGATATT GCTGGGCAGG CCCTGGCTTT TGTTCAGGAT 
CTTGTGACGG CTCTTCTAAA CTTTCATACC TACACAGAAC AGAGGATTCA AATTTTTCCT 
GTTGATTCTG CCATTGACAC TATATCTCCA TTGAATCAGA AGTTCTCACA ATACCTTCAT 
GAAAATGCGT CCTATGTCCG CCCTCTTGAG GAAGGAATGC TTCATTTATT TGAAAGTATC 
ACTGAGGATA CTGTGACTGT CTTGGAGACA ACTGTGAAAT TGAAAACTTT TTCAGAACAC 
TTAACCTCCT ACATATGTTT TCTTAGGAAG ATTCTTCCCT ATCAGTTAAA AAGTTTAGAA 
GAAGAATGTG AATCCTCTCT TTGCACATCT GCGTTAAGAG CCAGGAATCT AGAGCTGTCC 
CAGGACATGA AAAAAATGAC AGCTGTGTTT GAGAAGCTGC AGACTTACAT AGCTCTTCTT 
GCCTTGCCAA GTACAGAGCC AGATGGACTC CTTCGGACAA ACTACAGTTC TGTGTTAACA 
AATGTTGGTG CTGCTCTGCA TGGATTTCAT GACGTTATGA AAGATATTTC CAAACATTAT 
AGTCAAAAAG CTGCAATAGA GCATGAACTT CCAACAGCAA CACAGAAGCT GATAACAACT 
AATGACTGTA TCCTGTCATC AGTAGTGGCA TTAACAAATG GAGCAGGAAA GATTGCATCC 
TTCTTCAGCA ACAATTTGGA CTACTTCATT GCTTCACTGA GCTATGGACC TAAGGCAGCG 
AGTGGATTCA TTAGTCCTCT TTCAGCTGAA TGCATGCTAC AGTATAAGAA AAAAGCTGCT 
GCCTATATGA AGTCTTTGAG AAAGCCCCTC TTGGAGTCTG TGCCTTATGA AGAAGCACTG 
GCAAACCGCC GCATCCTTCT CAGCTCTACT GAAAGTCGAG AAGGCCTTGC ACAGCAAGTT 
CAACAGAGTT TGGAAAAGAT TTCTAAACTG GAGCAGGAAA AAGAACATTG GATGTTGGAA 
GCACAATTAG CCAAAATCAA GCTAGAGAAA GAAAACCAGC GAATTGCAGA TAAGCTGAAG 
AATACAGGTA GTGCCCAGCT GGTTGGGCTG GCCCAGGAAA ATGCTGCTGT GTCAAATACT 
GCTGGCCAGG ATGAAGCCAC AGCTAAGGCT GTGTTGGAGC CCATTCAGAG CACCAGTCTA 
GTTCCAGATG TGGAATCTCG TGAAGACTTA ATTAAAAATC ACTACATGGC AAGGATAGTG 
GAACTTACGT CTCAGTTGCA GCTGGCTGAC AGTAAGTCAG TGCATTTTTA TGCCGAGTGC 
CGAGCACTGT CTAAAAGACT GGCCTTGGCT GAAAAGTCTA AGGAAGCATT GACAGAAGAA 
ATGAAACTTG CCAGTCAGAA CATCAGCAGA CTTCAGGATG AGCTGACAAC TACCAAGAGG 
AGTTACGAGG ATCAGTTAAG TATGATGAGT GACCACCTGT GCAGCATGAA TGAGACATTA 
TCTAAACAGA GAGAAGAGAT TGACACACTA AAGATGTCCA GTAAGGGGAA TTCTAAAAAG 
AACAAGAGTC GATAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

PubMed

book

Defining the membrane proteome of NK cells.
Journal of mass spectrometry : JMS45(1)1-25(2010 Jan)
Ghosh D,Lippert D,Krokhin O,Cortens JP,Wilkins JA


book

Docking motif-guided mapping of the interactome of protein phosphatase-1.
Chemistry & biology16(4)365-71(2009 Apr)
Hendrickx A,Beullens M,Ceulemans H,Den Abt T,Van Eynde A,Nicolaescu E,Lesage B,Bollen M