NEK10 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Gene Symbol	NEK10
Entrez Gene ID	152110
Full Name	NIMA related kinase 10
General protein information	Preferred Names NIMA related kinase 10 Names serine/threonine-protein kinase Nek10 NIMA (never in mitosis gene a)- related kinase 10 never in mitosis A-related kinase 10 nimA-related protein kinase 10
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 3 Map Location: 3p24.1

mRNA and Protein(s)

mRNA	Protein	Name
XM_017005766.1	XP_016861255.1	serine/threonine-protein kinase Nek10 isoform X2
XM_017005767.1	XP_016861256.1	serine/threonine-protein kinase Nek10 isoform X5
XM_017005764.1	XP_016861253.1	serine/threonine-protein kinase Nek10 isoform X1
XM_017005766.2	XP_016861255.1	serine/threonine-protein kinase Nek10 isoform X2
XM_006713001.3	XP_006713064.1	serine/threonine-protein kinase Nek10 isoform X8
XM_017005772.2	XP_016861261.1	serine/threonine-protein kinase Nek10 isoform X9
NM_001304384.1	NP_001291313.1	serine/threonine-protein kinase Nek10 isoform 4
NM_199347.4	NP_955379.2	serine/threonine-protein kinase Nek10 isoform 3
XM_017005762.2	XP_016861251.1	serine/threonine-protein kinase Nek10 isoform X1
XM_017005768.1	XP_016861257.1	serine/threonine-protein kinase Nek10 isoform X6
NM_152534.4	NP_689747.3	serine/threonine-protein kinase Nek10 isoform 2
XM_017005771.1	XP_016861260.1	serine/threonine-protein kinase Nek10 isoform X8
XM_017005761.1	XP_016861250.1	serine/threonine-protein kinase Nek10 isoform X1
NM_199347.3	NP_955379.2	serine/threonine-protein kinase Nek10 isoform 3
XM_024453373.1	XP_024309141.1	serine/threonine-protein kinase Nek10 isoform X5
XM_017005771.2	XP_016861260.1	serine/threonine-protein kinase Nek10 isoform X8
XM_017005774.2	XP_016861263.1	serine/threonine-protein kinase Nek10 isoform X11
XM_006713002.1	XP_006713065.1	serine/threonine-protein kinase Nek10 isoform X13
XM_011533414.2	XP_011531716.1	serine/threonine-protein kinase Nek10 isoform X14
XM_017005772.1	XP_016861261.1	serine/threonine-protein kinase Nek10 isoform X9
XM_017005763.1	XP_016861252.1	serine/threonine-protein kinase Nek10 isoform X1
XM_017005769.1	XP_016861258.1	serine/threonine-protein kinase Nek10 isoform X7
XM_017005773.1	XP_016861262.1	serine/threonine-protein kinase Nek10 isoform X10
XM_024453374.1	XP_024309142.1	serine/threonine-protein kinase Nek10 isoform X12
NM_001031741.3	NP_001026911.1	serine/threonine-protein kinase Nek10 isoform 1
XM_017005774.1	XP_016861263.1	serine/threonine-protein kinase Nek10 isoform X11
XM_017005770.1	XP_016861259.1	serine/threonine-protein kinase Nek10 isoform X7
XM_017005762.1	XP_016861251.1	serine/threonine-protein kinase Nek10 isoform X1
XM_017005761.2	XP_016861250.1	serine/threonine-protein kinase Nek10 isoform X1
XM_017005765.1	XP_016861254.1	serine/threonine-protein kinase Nek10 isoform X1
XM_006712997.3	XP_006713060.1	serine/threonine-protein kinase Nek10 isoform X2
XM_006712998.2	XP_006713061.1	serine/threonine-protein kinase Nek10 isoform X3
XM_006712999.3	XP_006713062.1	serine/threonine-protein kinase Nek10 isoform X4
XM_017005767.2	XP_016861256.1	serine/threonine-protein kinase Nek10 isoform X5
XM_017005770.2	XP_016861259.1	serine/threonine-protein kinase Nek10 isoform X7
XM_011533413.2	XP_011531715.1	serine/threonine-protein kinase Nek10 isoform X11
XM_011533415.1	XP_011531717.1	serine/threonine-protein kinase Nek10 isoform X15

Pathways from BioSystems
Homologies

Pan troglodytes (chimpanzee) NEK10 XP_001165108.1

Homo sapiens (human) NEK10 NP_955379.2
Gene Ontology
Bibliography

Related articles in PubMed

Nek10 mediates G2/M cell cycle arrest and MEK autoactivation in response to UV irradiation.
Moniz LS, Stambolic V
Molecular and cellular biology31(1)30-42(2011 Jan)

Newly discovered breast cancer susceptibility loci on 3p24 and 17q23.2.
Ahmed S, Thomas G, Ghoussaini M, Healey CS, Humphreys MK, Platte R, Morrison J, Maranian M, Pooley KA, Luben R, Eccles D, Evans DG, Fletcher O, Johnson N, dos Santos Silva I, Peto J, Stratton MR, Rahman N, Jacobs K, Prentice R, Anderson GL, Rajkovic A, Curb JD, Ziegler RG, Berg CD, Buys SS, McCarty CA, Feigelson HS, Calle EE, Thun MJ, Diver WR, Bojesen S, Nordestgaard BG, Flyger H, D?rk T, Sch?rmann P, Hillemanns P, Karstens JH, Bogdanova NV, Antonenkova NN, Zalutsky IV, Bermisheva M, Fedorova S, Khusnutdinova E, , Kang D, Yoo KY, Noh DY, Ahn SH, Devilee P, van Asperen CJ, Tollenaar RA, Seynaeve C, Garcia-Closas M, Lissowska J, Brinton L, Peplonska B, Nevanlinna H, Heikkinen T, Aittom?ki K, Blomqvist C, Hopper JL, Southey MC, Smith L, Spurdle AB, Schmidt MK, Broeks A, van Hien RR, Cornelissen S, Milne RL, Ribas G, Gonz?lez-Neira A, Benitez J, Schmutzler RK, Burwinkel B, Bartram CR, Meindl A, Brauch H, Justenhoven C, Hamann U, , Chang-Claude J, Hein R, Wang-Gohrke S, Lindblom A, Margolin S, Mannermaa A, Kosma VM, Kataja V, Olson JE, Wang X, Fredericksen Z, Giles GG, Severi G, Baglietto L, English DR, Hankinson SE, Cox DG, Kraft P, Vatten LJ, Hveem K, Kumle M, Sigurdson A, Doody M, Bhatti P, Alexander BH, Hooning MJ, van den Ouweland AM, Oldenburg RA, Schutte M, Hall P, Czene K, Liu J, Li Y, Cox A, Elliott G, Brock I, Reed MW, Shen CY, Yu JC, Hsu GC, Chen ST, Anton-Culver H, Ziogas A, Andrulis IL, Knight JA, , , Beesley J, Goode EL, Couch F, Chenevix-Trench G, Hoover RN, Ponder BA, Hunter DJ, Pharoah PD, Dunning AM, Chanock SJ, Easton DF
Nature genetics41(5)585-90(2009 May)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Nek10 physically associated with Raf-1 and MEK1 in a Raf-1-dependent manner, and the formation of this complex was necessary for Nek10-mediated MEK1 activation.
Title: Nek10 mediates G2/M cell cycle arrest and MEK autoactivation in response to UV irradiation.

Clinical trial of gene-disease association and gene-environment interaction. (HuGE Navigator)
Title: Personalized smoking cessation: interactions between nicotine dose, dependence and quit-success genotype score.

Observational study of gene-disease association and gene-environment interaction. (HuGE Navigator)
Title: Novel breast cancer risk alleles and interaction with ionizing radiation among U.S. radiologic technologists.

Observational study of gene-disease association and gene-environment interaction. (HuGE Navigator)
Title: Common genetic variants associated with breast cancer and mammographic density measures that predict disease.

Observational study of gene-disease association. (HuGE Navigator)
Title: Newly discovered breast cancer susceptibility loci on 3p24 and 17q23.2.

The following NEK10 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the NEK10 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

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***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu31682	NM_199347.4 Latest version!	Homo sapiens NIMA related kinase 10 (NEK10), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$354.50-$496.30 $709.00
OHu76710	NM_001031741.3 Latest version!	Homo sapiens NIMA related kinase 10 (NEK10), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$349.30 $499.00
OHu76711	NM_001304384.1 Latest version!	Homo sapiens NIMA related kinase 10 (NEK10), transcript variant 4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$223.30 $319.00
OHu76709	XM_011533415.1 Latest version!	Homo sapiens NIMA related kinase 10 (NEK10), transcript variant X25, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$307.30 $439.00
OHu36997	XM_017005774.2 Latest version!	Homo sapiens NIMA related kinase 10 (NEK10), transcript variant X21, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$391.30 $559.00
OHu36997	XM_011533413.2 Latest version!	Homo sapiens NIMA related kinase 10 (NEK10), transcript variant X20, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$391.30 $559.00
OHu36998	XM_006713002.1 Latest version!	Homo sapiens NIMA related kinase 10 (NEK10), transcript variant X23, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$391.30 $559.00
OHu76708	XM_011533414.2 Latest version!	Homo sapiens NIMA related kinase 10 (NEK10), transcript variant X24, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$391.30 $559.00
OHu105312	XM_024453374.1 Latest version!	Homo sapiens NIMA related kinase 10 (NEK10), transcript variant X22, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
OHu93839	XM_017005773.1 Latest version!	Homo sapiens NIMA related kinase 10 (NEK10), transcript variant X19, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
OHu93835	XM_017005767.2 Latest version!	Homo sapiens NIMA related kinase 10 (NEK10), transcript variant X12, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OHu93835	XM_024453373.1 Latest version!	Homo sapiens NIMA related kinase 10 (NEK10), transcript variant X11, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OHu93836	XM_017005768.1 Latest version!	Homo sapiens NIMA related kinase 10 (NEK10), transcript variant X13, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OHu93837	XM_017005769.1 Latest version!	Homo sapiens NIMA related kinase 10 (NEK10), transcript variant X14, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OHu93837	XM_017005770.2 Latest version!	Homo sapiens NIMA related kinase 10 (NEK10), transcript variant X15, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OHu93838	XM_017005772.2 Latest version!	Homo sapiens NIMA related kinase 10 (NEK10), transcript variant X18, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OHu36991	NM_152534.4 Latest version!	Homo sapiens NIMA related kinase 10 (NEK10), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$643.30 $919.00
OHu36991	XM_017005765.1 Latest version!	Homo sapiens NIMA related kinase 10 (NEK10), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$643.30 $919.00
OHu36991	XM_017005764.1 Latest version!	Homo sapiens NIMA related kinase 10 (NEK10), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$643.30 $919.00
OHu36991	XM_017005763.1 Latest version!	Homo sapiens NIMA related kinase 10 (NEK10), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$643.30 $919.00
OHu36991	XM_017005762.2 Latest version!	Homo sapiens NIMA related kinase 10 (NEK10), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$643.30 $919.00
OHu36991	XM_017005761.2 Latest version!	Homo sapiens NIMA related kinase 10 (NEK10), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$643.30 $919.00
OHu36992	XM_006712997.3 Latest version!	Homo sapiens NIMA related kinase 10 (NEK10), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$559.30 $799.00
OHu36992	XM_017005766.2 Latest version!	Homo sapiens NIMA related kinase 10 (NEK10), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$559.30 $799.00
OHu36993	XM_006712998.2 Latest version!	Homo sapiens NIMA related kinase 10 (NEK10), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$559.30 $799.00
OHu36994	XM_006712999.3 Latest version!	Homo sapiens NIMA related kinase 10 (NEK10), transcript variant X10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$559.30 $799.00
OHu36996	XM_006713001.3 Latest version!	Homo sapiens NIMA related kinase 10 (NEK10), transcript variant X16, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$559.30 $799.00
OHu36996	XM_017005771.2 Latest version!	Homo sapiens NIMA related kinase 10 (NEK10), transcript variant X17, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$559.30 $799.00
View Old Accession Versions >>
OHu36992	XM_017005766.1	Homo sapiens NIMA related kinase 10 (NEK10), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$559.30 $799.00
OHu31682	NM_199347.3	Homo sapiens NIMA related kinase 10 (NEK10), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$354.50-$496.30 $709.00
OHu36996	XM_017005771.1	Homo sapiens NIMA related kinase 10 (NEK10), transcript variant X16, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$559.30 $799.00
OHu93838	XM_017005772.1	Homo sapiens NIMA related kinase 10 (NEK10), transcript variant X17, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OHu36997	XM_017005774.1	Homo sapiens NIMA related kinase 10 (NEK10), transcript variant X20, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$391.30 $559.00
OHu36991	XM_017005762.1	Homo sapiens NIMA related kinase 10 (NEK10), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$643.30 $919.00
OHu36991	XM_017005761.1	Homo sapiens NIMA related kinase 10 (NEK10), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$643.30 $919.00
OHu93835	XM_017005767.1	Homo sapiens NIMA related kinase 10 (NEK10), transcript variant X11, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OHu93837	XM_017005770.1	Homo sapiens NIMA related kinase 10 (NEK10), transcript variant X14, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

NEK10 ( NM_199347.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_955379.2 Homo sapiens NIMA related kinase 10 (NEK10), transcript variant 3, mRNA.

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CloneID	OHu31682
Clone ID Related Accession (Same CDS sequence)	NM_199347.4 , NM_199347.3
Accession Version	NM_199347.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2139bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-29
Organism	Homo sapiens(human)
Product	serine/threonine-protein kinase Nek10 isoform 3
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AK123061.1, BX537852.1 and AK098832.1. On Jun 2, 2019 this sequence version replaced NM_199347.3. Transcript Variant: This variant (3) lacks multiple 3' coding exons and contains an alternate 3' terminal exon, compared to variant 2, resulting in a different 3' coding region and 3' UTR. The encoded isoform (3) has a distinct C-terminus and is shorter than isoform 2. ##Evidence-Data-START## Transcript exon combination :: AK123061.1 [ECO:0000332] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGCCTGATC AAGATAAAAA GGTGAAGACC ACAGAAAAAT CAACTGATAA ACAGCAAGAA 
ATCACCATCA GGGACTATTC AGATCTTAAA AGACTTCGGT GCCTTTTGAA CGTCCAATCA 
AGCAAACAAC AGCTTCCAGC CATTAACTTC GATAGTGCCC AAAATAGCAT GACGAAGTCT 
GAGCCCGCCA TCAGGGCGGG TGGACACAGA GCTCGGGGTC AGTGGCATGA ATCCACAGAA 
GCTGTTGAAC TTGAAAATTT TAGTATAAAC TACAAGAATG AGAGAAATTT CAGCAAACAT 
CCTCAGCGTA AACTATTTCA GGAGATCTTT ACCGCCTTGG TGAAAAATAG ACTCATAAGC 
AGAGAGTGGG TTAATCGAGC CCCATCTATT CATTTTCTGA GAGTGTTAAT CTGTCTGAGG 
CTACTAATGA GGGATCCATG TTATCAGGAA ATACTCCATA GCTTGGGTGG GATTGAAAAC 
CTAGCTCAGT ATATGGAGAT TGTAGCCAAT GAGTACCTCG GCTATGGAGA AGAGCAGCAC 
ACTGTGGACA AGCTGGTCAA CATGACATAT ATTTTTCAAA AACTTGCTGC AGTCAAAGAT 
CAAAGAGAAT GGGTCACCAC AAGTGGAGCC CACAAGACAT TAGTAAATTT ACTTGGTGCC 
CGAGATACTA ATGTTCTATT GGGTTCCCTT CTGGCTCTGG CTAGTTTAGC AGAAAGTCAA 
GAATGTAGGG AGAAGATAAG TGAACTCAAC ATTGTAGAAA ATCTGTTGAT GATTTTACAT 
GAATATGACT TGCTTTCTAA AAGACTAACA GCGGAGTTGC TGCGCCTACT TTGTGCAGAG 
CCCCAGGTGA AAGAGCAGGT GAAGCTCTAT GAGGGGATAC CGGTCCTCCT CAGTCTGCTC 
CACTCTGACC ACTTGAAGCT CCTCTGGAGC ATTGTCTGGA TTCTGGTACA GGTTTGTGAG 
GACCCTGAGA CCAGCGTGGA AATTCGCATT TGGGGAGGCA TCAAACAGCT TCTTCATATT 
TTACAAGGAG ACAGAAATTT TGTTTCTGAT CACTCCTCCA TTGGAAGCCT GTCCAGTGCA 
AATGCTGCAG GCCGAATCCA GCAGCTTCAT TTATCAGAAG ACTTGAGCCC TAGGGAAATA 
CAAGAAAATA CTTTCTCACT TCAAGCAGCC TGCTGTGCTG CCCTCACTGA GCTGGTGCTC 
AATGACACCA ATGCCCACCA GGTGGTTCAG GAAAATGGTG TATATACAAT AGCAAAATTA 
ATTTTACCAA ATAAGCAAAA GAATGCAGCA AAAAGTAATC TATTACAGTG TTATGCTTTC 
AGAGCCTTGA GATTTCTCTT CAGTATGGAA AGAAACAGAC CACTCTTTAA AAGACTTTTC 
CCCACAGACT TGTTTGAGAT CTTCATTGAC ATAGGGCATT ATGTACGTGA TATCAGTGCT 
TATGAAGAAT TGGTATCCAA GCTGAATTTA TTAGTGGAGG ATGAACTGAA GCAAATTGCT 
GAAAATATTG AAAGCATTAA TCAGAACAAA GCTCCTTTGA AATATATAGG CAACTATGCA 
ATTTTGGATC ATCTTGGAAG TGGAGCTTTT GGCTGTGTTT ACAAGGTTAG AAAGCATAGT 
GGTCAAAATC TTTTAGCAAT GAAAGAGGTC AATTTACATA ACCCAGCATT TGGAAAGGAT 
AAGAAAGATC GAGACAGCAG CGTAAGGAAT ATTGTTTCTG AATTAACAAT AATTAAAGAG 
CAGCTTTATC ATCCCAACAT TGTACGTTAT TACAAAACAT TTCTGGAAAA TGATAGGTTG 
TACATAGTTA TGGAGCTGAT AGAAGGAGCC CCGCTTGGAG AGCATTTCAG TTCTTTGAAG 
GAAAAACATC ACCATTTTAC TGAAGAAAGA CTATGGAAAA TATTTATACA GCTGTGCTTA 
GCTCTTCGAT ACTTACACAA GGAGAAGAGG ATTGTCCATA GAGATCTGAC ACCAAACAAC 
ATTATGTTGG GGGATAAGGA CAAAGTAACC GTTACTGACT TTGGCCTGGC AAAGCAAAAA 
CAAGAAAACA GTAAACTCAC CTCTGTGGTT GGAACAATCC TGTATTCTTG TGTGCAGCAC 
CTCTACCTTC GCTCTCCTGC TCCTGCTCTG GCCACATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_955379.2
CDS	237..2375
Translation	MPDQDKKVKTTEKSTDKQQEITIRDYSDLKRLRCLLNVQSSKQQLPAINFDSAQNSMTKSEPAIRAGGHRARGQWHESTEAVELENFSINYKNERNFSKHPQRKLFQEIFTALVKNRLISREWVNRAPSIHFLRVLICLRLLMRDPCYQEILHSLGGIENLAQYMEIVANEYLGYGEEQHTVDKLVNMTYIFQKLAAVKDQREWVTTSGAHKTLVNLLGARDTNVLLGSLLALASLAESQECREKISELNIVENLLMILHEYDLLSKRLTAELLRLLCAEPQVKEQVKLYEGIPVLLSLLHSDHLKLLWSIVWILVQVCEDPETSVEIRIWGGIKQLLHILQGDRNFVSDHSSIGSLSSANAAGRIQQLHLSEDLSPREIQENTFSLQAACCAALTELVLNDTNAHQVVQENGVYTIAKLILPNKQKNAAKSNLLQCYAFRALRFLFSMERNRPLFKRLFPTDLFEIFIDIGHYVRDISAYEELVSKLNLLVEDELKQIAENIESINQNKAPLKYIGNYAILDHLGSGAFGCVYKVRKHSGQNLLAMKEVNLHNPAFGKDKKDRDSSVRNIVSELTIIKEQLYHPNIVRYYKTFLENDRLYIVMELIEGAPLGEHFSSLKEKHHHFTEERLWKIFIQLCLALRYLHKEKRIVHRDLTPNNIMLGDKDKVTVTDFGLAKQKQENSKLTSVVGTILYSCVQHLYLRSPAPALAT

Target ORF information:

RefSeq Version	NM_199347.4
Organism	Homo sapiens(human)
Definition	Homo sapiens NIMA related kinase 10 (NEK10), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_199347.4

ORF Insert Sequence:

ATGCCTGATC AAGATAAAAA GGTGAAGACC ACAGAAAAAT CAACTGATAA ACAGCAAGAA 
ATCACCATCA GGGACTATTC AGATCTTAAA AGACTTCGGT GCCTTTTGAA CGTCCAATCA 
AGCAAACAAC AGCTTCCAGC CATTAACTTC GATAGTGCCC AAAATAGCAT GACGAAGTCT 
GAGCCCGCCA TCAGGGCGGG TGGACACAGA GCTCGGGGTC AGTGGCATGA ATCCACAGAA 
GCTGTTGAAC TTGAAAATTT TAGTATAAAC TACAAGAATG AGAGAAATTT CAGCAAACAT 
CCTCAGCGTA AACTATTTCA GGAGATCTTT ACCGCCTTGG TGAAAAATAG ACTCATAAGC 
AGAGAGTGGG TTAATCGAGC CCCATCTATT CATTTTCTGA GAGTGTTAAT CTGTCTGAGG 
CTACTAATGA GGGATCCATG TTATCAGGAA ATACTCCATA GCTTGGGTGG GATTGAAAAC 
CTAGCTCAGT ATATGGAGAT TGTAGCCAAT GAGTACCTCG GCTATGGAGA AGAGCAGCAC 
ACTGTGGACA AGCTGGTCAA CATGACATAT ATTTTTCAAA AACTTGCTGC AGTCAAAGAT 
CAAAGAGAAT GGGTCACCAC AAGTGGAGCC CACAAGACAT TAGTAAATTT ACTTGGTGCC 
CGAGATACTA ATGTTCTATT GGGTTCCCTT CTGGCTCTGG CTAGTTTAGC AGAAAGTCAA 
GAATGTAGGG AGAAGATAAG TGAACTCAAC ATTGTAGAAA ATCTGTTGAT GATTTTACAT 
GAATATGACT TGCTTTCTAA AAGACTAACA GCGGAGTTGC TGCGCCTACT TTGTGCAGAG 
CCCCAGGTGA AAGAGCAGGT GAAGCTCTAT GAGGGGATAC CGGTCCTCCT CAGTCTGCTC 
CACTCTGACC ACTTGAAGCT CCTCTGGAGC ATTGTCTGGA TTCTGGTACA GGTTTGTGAG 
GACCCTGAGA CCAGCGTGGA AATTCGCATT TGGGGAGGCA TCAAACAGCT TCTTCATATT 
TTACAAGGAG ACAGAAATTT TGTTTCTGAT CACTCCTCCA TTGGAAGCCT GTCCAGTGCA 
AATGCTGCAG GCCGAATCCA GCAGCTTCAT TTATCAGAAG ACTTGAGCCC TAGGGAAATA 
CAAGAAAATA CTTTCTCACT TCAAGCAGCC TGCTGTGCTG CCCTCACTGA GCTGGTGCTC 
AATGACACCA ATGCCCACCA GGTGGTTCAG GAAAATGGTG TATATACAAT AGCAAAATTA 
ATTTTACCAA ATAAGCAAAA GAATGCAGCA AAAAGTAATC TATTACAGTG TTATGCTTTC 
AGAGCCTTGA GATTTCTCTT CAGTATGGAA AGAAACAGAC CACTCTTTAA AAGACTTTTC 
CCCACAGACT TGTTTGAGAT CTTCATTGAC ATAGGGCATT ATGTACGTGA TATCAGTGCT 
TATGAAGAAT TGGTATCCAA GCTGAATTTA TTAGTGGAGG ATGAACTGAA GCAAATTGCT 
GAAAATATTG AAAGCATTAA TCAGAACAAA GCTCCTTTGA AATATATAGG CAACTATGCA 
ATTTTGGATC ATCTTGGAAG TGGAGCTTTT GGCTGTGTTT ACAAGGTTAG AAAGCATAGT 
GGTCAAAATC TTTTAGCAAT GAAAGAGGTC AATTTACATA ACCCAGCATT TGGAAAGGAT 
AAGAAAGATC GAGACAGCAG CGTAAGGAAT ATTGTTTCTG AATTAACAAT AATTAAAGAG 
CAGCTTTATC ATCCCAACAT TGTACGTTAT TACAAAACAT TTCTGGAAAA TGATAGGTTG 
TACATAGTTA TGGAGCTGAT AGAAGGAGCC CCGCTTGGAG AGCATTTCAG TTCTTTGAAG 
GAAAAACATC ACCATTTTAC TGAAGAAAGA CTATGGAAAA TATTTATACA GCTGTGCTTA 
GCTCTTCGAT ACTTACACAA GGAGAAGAGG ATTGTCCATA GAGATCTGAC ACCAAACAAC 
ATTATGTTGG GGGATAAGGA CAAAGTAACC GTTACTGACT TTGGCCTGGC AAAGCAAAAA 
CAAGAAAACA GTAAACTCAC CTCTGTGGTT GGAACAATCC TGTATTCTTG TGTGCAGCAC 
CTCTACCTTC GCTCTCCTGC TCCTGCTCTG GCCACATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NEK10 ( NM_199347.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_955379.2 Homo sapiens NIMA related kinase 10 (NEK10), transcript variant 3, mRNA.

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CloneID	OHu31682
Clone ID Related Accession (Same CDS sequence)	NM_199347.4 , NM_199347.3
Accession Version	NM_199347.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2139bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-29
Organism	Homo sapiens(human)
Product	serine/threonine-protein kinase Nek10 isoform 3
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from DB094082.1, AK123061.1, BX537852.1 and AK098832.1. On Jan 22, 2015 this sequence version replaced NM_199347.2. Transcript Variant: This variant (3) lacks multiple 3' coding exons and contains an alternate 3' terminal exon, compared to variant 2, resulting in a different 3' coding region and 3' UTR. The encoded isoform (3) has a distinct C-terminus and is shorter than isoform 2. ##Evidence-Data-START## Transcript exon combination :: AK123061.1 [ECO:0000332] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGCCTGATC AAGATAAAAA GGTGAAGACC ACAGAAAAAT CAACTGATAA ACAGCAAGAA 
ATCACCATCA GGGACTATTC AGATCTTAAA AGACTTCGGT GCCTTTTGAA CGTCCAATCA 
AGCAAACAAC AGCTTCCAGC CATTAACTTC GATAGTGCCC AAAATAGCAT GACGAAGTCT 
GAGCCCGCCA TCAGGGCGGG TGGACACAGA GCTCGGGGTC AGTGGCATGA ATCCACAGAA 
GCTGTTGAAC TTGAAAATTT TAGTATAAAC TACAAGAATG AGAGAAATTT CAGCAAACAT 
CCTCAGCGTA AACTATTTCA GGAGATCTTT ACCGCCTTGG TGAAAAATAG ACTCATAAGC 
AGAGAGTGGG TTAATCGAGC CCCATCTATT CATTTTCTGA GAGTGTTAAT CTGTCTGAGG 
CTACTAATGA GGGATCCATG TTATCAGGAA ATACTCCATA GCTTGGGTGG GATTGAAAAC 
CTAGCTCAGT ATATGGAGAT TGTAGCCAAT GAGTACCTCG GCTATGGAGA AGAGCAGCAC 
ACTGTGGACA AGCTGGTCAA CATGACATAT ATTTTTCAAA AACTTGCTGC AGTCAAAGAT 
CAAAGAGAAT GGGTCACCAC AAGTGGAGCC CACAAGACAT TAGTAAATTT ACTTGGTGCC 
CGAGATACTA ATGTTCTATT GGGTTCCCTT CTGGCTCTGG CTAGTTTAGC AGAAAGTCAA 
GAATGTAGGG AGAAGATAAG TGAACTCAAC ATTGTAGAAA ATCTGTTGAT GATTTTACAT 
GAATATGACT TGCTTTCTAA AAGACTAACA GCGGAGTTGC TGCGCCTACT TTGTGCAGAG 
CCCCAGGTGA AAGAGCAGGT GAAGCTCTAT GAGGGGATAC CGGTCCTCCT CAGTCTGCTC 
CACTCTGACC ACTTGAAGCT CCTCTGGAGC ATTGTCTGGA TTCTGGTACA GGTTTGTGAG 
GACCCTGAGA CCAGCGTGGA AATTCGCATT TGGGGAGGCA TCAAACAGCT TCTTCATATT 
TTACAAGGAG ACAGAAATTT TGTTTCTGAT CACTCCTCCA TTGGAAGCCT GTCCAGTGCA 
AATGCTGCAG GCCGAATCCA GCAGCTTCAT TTATCAGAAG ACTTGAGCCC TAGGGAAATA 
CAAGAAAATA CTTTCTCACT TCAAGCAGCC TGCTGTGCTG CCCTCACTGA GCTGGTGCTC 
AATGACACCA ATGCCCACCA GGTGGTTCAG GAAAATGGTG TATATACAAT AGCAAAATTA 
ATTTTACCAA ATAAGCAAAA GAATGCAGCA AAAAGTAATC TATTACAGTG TTATGCTTTC 
AGAGCCTTGA GATTTCTCTT CAGTATGGAA AGAAACAGAC CACTCTTTAA AAGACTTTTC 
CCCACAGACT TGTTTGAGAT CTTCATTGAC ATAGGGCATT ATGTACGTGA TATCAGTGCT 
TATGAAGAAT TGGTATCCAA GCTGAATTTA TTAGTGGAGG ATGAACTGAA GCAAATTGCT 
GAAAATATTG AAAGCATTAA TCAGAACAAA GCTCCTTTGA AATATATAGG CAACTATGCA 
ATTTTGGATC ATCTTGGAAG TGGAGCTTTT GGCTGTGTTT ACAAGGTTAG AAAGCATAGT 
GGTCAAAATC TTTTAGCAAT GAAAGAGGTC AATTTACATA ACCCAGCATT TGGAAAGGAT 
AAGAAAGATC GAGACAGCAG CGTAAGGAAT ATTGTTTCTG AATTAACAAT AATTAAAGAG 
CAGCTTTATC ATCCCAACAT TGTACGTTAT TACAAAACAT TTCTGGAAAA TGATAGGTTG 
TACATAGTTA TGGAGCTGAT AGAAGGAGCC CCGCTTGGAG AGCATTTCAG TTCTTTGAAG 
GAAAAACATC ACCATTTTAC TGAAGAAAGA CTATGGAAAA TATTTATACA GCTGTGCTTA 
GCTCTTCGAT ACTTACACAA GGAGAAGAGG ATTGTCCATA GAGATCTGAC ACCAAACAAC 
ATTATGTTGG GGGATAAGGA CAAAGTAACC GTTACTGACT TTGGCCTGGC AAAGCAAAAA 
CAAGAAAACA GTAAACTCAC CTCTGTGGTT GGAACAATCC TGTATTCTTG TGTGCAGCAC 
CTCTACCTTC GCTCTCCTGC TCCTGCTCTG GCCACATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_955379.2
CDS	314..2452
Translation	MPDQDKKVKTTEKSTDKQQEITIRDYSDLKRLRCLLNVQSSKQQLPAINFDSAQNSMTKSEPAIRAGGHRARGQWHESTEAVELENFSINYKNERNFSKHPQRKLFQEIFTALVKNRLISREWVNRAPSIHFLRVLICLRLLMRDPCYQEILHSLGGIENLAQYMEIVANEYLGYGEEQHTVDKLVNMTYIFQKLAAVKDQREWVTTSGAHKTLVNLLGARDTNVLLGSLLALASLAESQECREKISELNIVENLLMILHEYDLLSKRLTAELLRLLCAEPQVKEQVKLYEGIPVLLSLLHSDHLKLLWSIVWILVQVCEDPETSVEIRIWGGIKQLLHILQGDRNFVSDHSSIGSLSSANAAGRIQQLHLSEDLSPREIQENTFSLQAACCAALTELVLNDTNAHQVVQENGVYTIAKLILPNKQKNAAKSNLLQCYAFRALRFLFSMERNRPLFKRLFPTDLFEIFIDIGHYVRDISAYEELVSKLNLLVEDELKQIAENIESINQNKAPLKYIGNYAILDHLGSGAFGCVYKVRKHSGQNLLAMKEVNLHNPAFGKDKKDRDSSVRNIVSELTIIKEQLYHPNIVRYYKTFLENDRLYIVMELIEGAPLGEHFSSLKEKHHHFTEERLWKIFIQLCLALRYLHKEKRIVHRDLTPNNIMLGDKDKVTVTDFGLAKQKQENSKLTSVVGTILYSCVQHLYLRSPAPALAT

Target ORF information:

RefSeq Version	NM_199347.3
Organism	Homo sapiens(human)
Definition	Homo sapiens NIMA related kinase 10 (NEK10), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_199347.3

ORF Insert Sequence:

ATGCCTGATC AAGATAAAAA GGTGAAGACC ACAGAAAAAT CAACTGATAA ACAGCAAGAA 
ATCACCATCA GGGACTATTC AGATCTTAAA AGACTTCGGT GCCTTTTGAA CGTCCAATCA 
AGCAAACAAC AGCTTCCAGC CATTAACTTC GATAGTGCCC AAAATAGCAT GACGAAGTCT 
GAGCCCGCCA TCAGGGCGGG TGGACACAGA GCTCGGGGTC AGTGGCATGA ATCCACAGAA 
GCTGTTGAAC TTGAAAATTT TAGTATAAAC TACAAGAATG AGAGAAATTT CAGCAAACAT 
CCTCAGCGTA AACTATTTCA GGAGATCTTT ACCGCCTTGG TGAAAAATAG ACTCATAAGC 
AGAGAGTGGG TTAATCGAGC CCCATCTATT CATTTTCTGA GAGTGTTAAT CTGTCTGAGG 
CTACTAATGA GGGATCCATG TTATCAGGAA ATACTCCATA GCTTGGGTGG GATTGAAAAC 
CTAGCTCAGT ATATGGAGAT TGTAGCCAAT GAGTACCTCG GCTATGGAGA AGAGCAGCAC 
ACTGTGGACA AGCTGGTCAA CATGACATAT ATTTTTCAAA AACTTGCTGC AGTCAAAGAT 
CAAAGAGAAT GGGTCACCAC AAGTGGAGCC CACAAGACAT TAGTAAATTT ACTTGGTGCC 
CGAGATACTA ATGTTCTATT GGGTTCCCTT CTGGCTCTGG CTAGTTTAGC AGAAAGTCAA 
GAATGTAGGG AGAAGATAAG TGAACTCAAC ATTGTAGAAA ATCTGTTGAT GATTTTACAT 
GAATATGACT TGCTTTCTAA AAGACTAACA GCGGAGTTGC TGCGCCTACT TTGTGCAGAG 
CCCCAGGTGA AAGAGCAGGT GAAGCTCTAT GAGGGGATAC CGGTCCTCCT CAGTCTGCTC 
CACTCTGACC ACTTGAAGCT CCTCTGGAGC ATTGTCTGGA TTCTGGTACA GGTTTGTGAG 
GACCCTGAGA CCAGCGTGGA AATTCGCATT TGGGGAGGCA TCAAACAGCT TCTTCATATT 
TTACAAGGAG ACAGAAATTT TGTTTCTGAT CACTCCTCCA TTGGAAGCCT GTCCAGTGCA 
AATGCTGCAG GCCGAATCCA GCAGCTTCAT TTATCAGAAG ACTTGAGCCC TAGGGAAATA 
CAAGAAAATA CTTTCTCACT TCAAGCAGCC TGCTGTGCTG CCCTCACTGA GCTGGTGCTC 
AATGACACCA ATGCCCACCA GGTGGTTCAG GAAAATGGTG TATATACAAT AGCAAAATTA 
ATTTTACCAA ATAAGCAAAA GAATGCAGCA AAAAGTAATC TATTACAGTG TTATGCTTTC 
AGAGCCTTGA GATTTCTCTT CAGTATGGAA AGAAACAGAC CACTCTTTAA AAGACTTTTC 
CCCACAGACT TGTTTGAGAT CTTCATTGAC ATAGGGCATT ATGTACGTGA TATCAGTGCT 
TATGAAGAAT TGGTATCCAA GCTGAATTTA TTAGTGGAGG ATGAACTGAA GCAAATTGCT 
GAAAATATTG AAAGCATTAA TCAGAACAAA GCTCCTTTGA AATATATAGG CAACTATGCA 
ATTTTGGATC ATCTTGGAAG TGGAGCTTTT GGCTGTGTTT ACAAGGTTAG AAAGCATAGT 
GGTCAAAATC TTTTAGCAAT GAAAGAGGTC AATTTACATA ACCCAGCATT TGGAAAGGAT 
AAGAAAGATC GAGACAGCAG CGTAAGGAAT ATTGTTTCTG AATTAACAAT AATTAAAGAG 
CAGCTTTATC ATCCCAACAT TGTACGTTAT TACAAAACAT TTCTGGAAAA TGATAGGTTG 
TACATAGTTA TGGAGCTGAT AGAAGGAGCC CCGCTTGGAG AGCATTTCAG TTCTTTGAAG 
GAAAAACATC ACCATTTTAC TGAAGAAAGA CTATGGAAAA TATTTATACA GCTGTGCTTA 
GCTCTTCGAT ACTTACACAA GGAGAAGAGG ATTGTCCATA GAGATCTGAC ACCAAACAAC 
ATTATGTTGG GGGATAAGGA CAAAGTAACC GTTACTGACT TTGGCCTGGC AAAGCAAAAA 
CAAGAAAACA GTAAACTCAC CTCTGTGGTT GGAACAATCC TGTATTCTTG TGTGCAGCAC 
CTCTACCTTC GCTCTCCTGC TCCTGCTCTG GCCACATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NEK10 ( NM_001031741.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_001026911.1 Homo sapiens NIMA related kinase 10 (NEK10), transcript variant 1, mRNA.

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CloneID	OHu76710
Clone ID Related Accession (Same CDS sequence)	NM_001031741.3
Accession Version	NM_001031741.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1425bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-29
Organism	Homo sapiens(human)
Product	serine/threonine-protein kinase Nek10 isoform 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from DB045668.1, BC045758.1 and AK098832.1. On Jan 22, 2015 this sequence version replaced NM_001031741.2. Transcript Variant: This variant (1) differs in the 5' UTR and contains multiple differences in the coding region compared to variant 2, including lacking multiple 5' coding exons. It initiates translation at an alternate start codon. The encoded isoform (1) has a distinct N-terminus and is shorter than isoform 1. ##Evidence-Data-START## Transcript exon combination :: BC045758.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGTTCCTG TACAGCCTGC AGAACCCCCC GAGGTACTGA AGAGTGAGCC GTATGGGGAG 
AAGGCTGATG TCTGGGCAGT AGGCTGCATC CTTTATCAGA TGGCGACTTT GAGTCCCCCC 
TTCTACAGCA CTAACATGCT GTCCTTGGCT ACAAAAATAG TGGAGGCGGT ATATGAACCA 
GTCCCAGAAG GTATCTACTC TGAAAAAGTA ACAGACACCA TCAGCAGGTG CCTCACTCCT 
GATGCGGAAG CTCGTCCAGA TATTGTAGAA GTCAGTTCGA TGATATCAGA TGTCATGATG 
AAATATTTAG ACAACTTATC TACATCCCAG TTGTCCTTGG AAAAGAAGCT AGAACGGGAA 
CGAAGACGCA CACAAAGGTA TTTTATGGAA GCCAACCGGA ACACCGTCAC ATGTCACCAT 
GAGCTGGCTG TTCTATCTCA CGAGACCTTT GAGAAGGCAA GTTTGAGTAG CAGCAGCAGT 
GGAGCAGCCA GCCTGAAAAG TGAACTTTCA GAAAGCGCAG ACCTGCCCCC TGAAGGCTTC 
CAGGCCTCCT ATGGTAAAGA CGAAGACAGG GCCTGTGACG AAATCCTGTC AGATGATAAC 
TTCAACCTGG AAAATGCTGA GAAAGATACA TATTCAGAGG TAGATGATGA ATTGGACATT 
TCGGATAACT CCAGCAGCTC CAGTTCAAGC CCTCTGAAAG AATCTACATT CAACATTTTA 
AAGAGAAGTT TTAGTGCTTC AGGAGGAGAA AGACAATCCC AAACAAGGGA CTTCACTGGA 
GGAACAGGAT CAAGACCAAG ACCAGCTTTG CTGCCTCTTG ACCTGCTTCT GAAAGTGCCA 
CCCCACATGC TCAGGGCCCA CATTAAGGAA ATAGAGGCTG AGTTAGTGAC AGGGTGGCAG 
TCCCATAGCC TTCCTGCTGT GATTCTTCGA AATCTCAAAG ATCATGCATC AGCAGGAATT 
GCTGTGTCCC AGAGGAAAGT GCGTCAGATC AGTGATCCTA TTCAGCAGAT ATTAATTCAG 
CTGCACAAAA TAATCTATAT CACACAGCTT CCTCCAGCTT TGCACCACAA TTTGAAAAGA 
AGGGTTATAG AGAGATTCAA GAAATCCCTC TTCAGCCAGC AGAGTAACCC TTGTAATTTG 
AAATCTGAAA TTAAAAAGTT ATCTCAGGGA TCTCCAGAAC CGATTGAGCC CAACTTTTTC 
ACAGCAGATT ACCATTTATT ACATCGTTCA TCCGGTGGAA ACAGCCTGTC CCCAAATGAC 
CCTACAGGTT TACCAACCAG CATTGAATTG GAGGAAGGAA TAACATATGA ACAGATGCAG 
ACTGTGATTG AAGAAGTCCT TGAGGAAAGT GGCTATTACA ATTTTACATC TAACAGGTAT 
CATTCCTATC CATGGGGGAC CAAGAATCAC CCAACCAAAA GATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001026911.1
CDS	278..1702
Translation	MVPVQPAEPPEVLKSEPYGEKADVWAVGCILYQMATLSPPFYSTNMLSLATKIVEAVYEPVPEGIYSEKVTDTISRCLTPDAEARPDIVEVSSMISDVMMKYLDNLSTSQLSLEKKLERERRRTQRYFMEANRNTVTCHHELAVLSHETFEKASLSSSSSGAASLKSELSESADLPPEGFQASYGKDEDRACDEILSDDNFNLENAEKDTYSEVDDELDISDNSSSSSSSPLKESTFNILKRSFSASGGERQSQTRDFTGGTGSRPRPALLPLDLLLKVPPHMLRAHIKEIEAELVTGWQSHSLPAVILRNLKDHASAGIAVSQRKVRQISDPIQQILIQLHKIIYITQLPPALHHNLKRRVIERFKKSLFSQQSNPCNLKSEIKKLSQGSPEPIEPNFFTADYHLLHRSSGGNSLSPNDPTGLPTSIELEEGITYEQMQTVIEEVLEESGYYNFTSNRYHSYPWGTKNHPTKR

Target ORF information:

RefSeq Version	NM_001031741.3
Organism	Homo sapiens(human)
Definition	Homo sapiens NIMA related kinase 10 (NEK10), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001031741.3

ORF Insert Sequence:

ATGGTTCCTG TACAGCCTGC AGAACCCCCC GAGGTACTGA AGAGTGAGCC GTATGGGGAG 
AAGGCTGATG TCTGGGCAGT AGGCTGCATC CTTTATCAGA TGGCGACTTT GAGTCCCCCC 
TTCTACAGCA CTAACATGCT GTCCTTGGCT ACAAAAATAG TGGAGGCGGT ATATGAACCA 
GTCCCAGAAG GTATCTACTC TGAAAAAGTA ACAGACACCA TCAGCAGGTG CCTCACTCCT 
GATGCGGAAG CTCGTCCAGA TATTGTAGAA GTCAGTTCGA TGATATCAGA TGTCATGATG 
AAATATTTAG ACAACTTATC TACATCCCAG TTGTCCTTGG AAAAGAAGCT AGAACGGGAA 
CGAAGACGCA CACAAAGGTA TTTTATGGAA GCCAACCGGA ACACCGTCAC ATGTCACCAT 
GAGCTGGCTG TTCTATCTCA CGAGACCTTT GAGAAGGCAA GTTTGAGTAG CAGCAGCAGT 
GGAGCAGCCA GCCTGAAAAG TGAACTTTCA GAAAGCGCAG ACCTGCCCCC TGAAGGCTTC 
CAGGCCTCCT ATGGTAAAGA CGAAGACAGG GCCTGTGACG AAATCCTGTC AGATGATAAC 
TTCAACCTGG AAAATGCTGA GAAAGATACA TATTCAGAGG TAGATGATGA ATTGGACATT 
TCGGATAACT CCAGCAGCTC CAGTTCAAGC CCTCTGAAAG AATCTACATT CAACATTTTA 
AAGAGAAGTT TTAGTGCTTC AGGAGGAGAA AGACAATCCC AAACAAGGGA CTTCACTGGA 
GGAACAGGAT CAAGACCAAG ACCAGCTTTG CTGCCTCTTG ACCTGCTTCT GAAAGTGCCA 
CCCCACATGC TCAGGGCCCA CATTAAGGAA ATAGAGGCTG AGTTAGTGAC AGGGTGGCAG 
TCCCATAGCC TTCCTGCTGT GATTCTTCGA AATCTCAAAG ATCATGCATC AGCAGGAATT 
GCTGTGTCCC AGAGGAAAGT GCGTCAGATC AGTGATCCTA TTCAGCAGAT ATTAATTCAG 
CTGCACAAAA TAATCTATAT CACACAGCTT CCTCCAGCTT TGCACCACAA TTTGAAAAGA 
AGGGTTATAG AGAGATTCAA GAAATCCCTC TTCAGCCAGC AGAGTAACCC TTGTAATTTG 
AAATCTGAAA TTAAAAAGTT ATCTCAGGGA TCTCCAGAAC CGATTGAGCC CAACTTTTTC 
ACAGCAGATT ACCATTTATT ACATCGTTCA TCCGGTGGAA ACAGCCTGTC CCCAAATGAC 
CCTACAGGTT TACCAACCAG CATTGAATTG GAGGAAGGAA TAACATATGA ACAGATGCAG 
ACTGTGATTG AAGAAGTCCT TGAGGAAAGT GGCTATTACA ATTTTACATC TAACAGGTAT 
CATTCCTATC CATGGGGGAC CAAGAATCAC CCAACCAAAA GATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NEK10 ( NM_001304384.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001291313.1 Homo sapiens NIMA related kinase 10 (NEK10), transcript variant 4, mRNA.

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CloneID	OHu76711
Clone ID Related Accession (Same CDS sequence)	NM_001304384.1
Accession Version	NM_001304384.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1455bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-29
Organism	Homo sapiens(human)
Product	serine/threonine-protein kinase Nek10 isoform 4
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from DB045668.1, AK057247.1, AC093555.2, AC098931.3 and BC045758.1. Transcript Variant: This variant (4) differs in the 5' UTR and lacks multiple 5' coding exons, compared to variant 2. It initiates translation at an alternate start codon. The encoded isoform (4) has a distinct N-terminus and is shorter than isoform 1. ##Evidence-Data-START## Transcript exon combination :: AK057247.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGTTCCTG TACAGCCTGC AGAACCCCCC GAGGTACTGA AGAGTGAGCC GTATGGGGAG 
AAGGCTGATG TCTGGGCAGT AGGCTGCATC CTTTATCAGA TGGCGACTTT GAGTCCCCCC 
TTCTACAGCA CTAACATGCT GTCCTTGGCT ACAAAAATAG TGGAGGCGGT ATATGAACCA 
GTCCCAGAAG GTATCTACTC TGAAAAAGTA ACAGACACCA TCAGCAGGTG CCTCACTCCT 
GATGCGGAAG CTCGTCCAGA TATTGTAGAA GTCAGTTCGA TGATATCAGA TGTCATGATG 
AAATATTTAG ACAACTTATC TACATCCCAG TTGTCCTTGG AAAAGAAGCT AGAACGGGAA 
CGAAGACGCA CACAAAGGTA TTTTATGGAA GCCAACCGGA ACACCGTCAC ATGTCACCAT 
GAGCTGGCTG TTCTATCTCA CGAGACCTTT GAGAAGGCAA GTTTGAGTAG CAGCAGCAGT 
GGAGCAGCCA GCCTGAAAAG TGAACTTTCA GAAAGCGCAG ACCTGCCCCC TGAAGGCTTC 
CAGGCCTCCT ATGGTAAAGA CGAAGACAGG GCCTGTGACG AAATCCTGTC AGATGATAAC 
TTCAACCTGG AAAATGCTGA GAAAGATACA TATTCAGAGG TAGATGATGA ATTGGACATT 
TCGGATAACT CCAGCAGCTC CAGTTCAAGC CCTCTGAAAG AATCTACATT CAACATTTTA 
AAGAGAAGTT TTAGTGCTTC AGGAGGAGAA AGACAATCCC AAACAAGGGA CTTCACTGGA 
GGAACAGGAT CAAGACCAAG ACCAGCTTTG CTGCCTCTTG ACCTGCTTCT GAAAGTGCCA 
CCCCACATGC TCAGGGCCCA CATTAAGGAA ATAGAGGCTG AGTTAGTGAC AGGGTGGCAG 
TCCCATAGCC TTCCTGCTGT GATTCTTCGA AATCTCAAAG ATCATGGGCC ACAGATGGGC 
ACATTCTTGT GGCAAGCATC AGCAGGAATT GCTGTGTCCC AGAGGAAAGT GCGTCAGATC 
AGTGATCCTA TTCAGCAGAT ATTAATTCAG CTGCACAAAA TAATCTATAT CACACAGCTT 
CCTCCAGCTT TGCACCACAA TTTGAAAAGA AGGGTTATAG AGAGATTCAA GAAATCCCTC 
TTCAGCCAGC AGAGTAACCC TTGTAATTTG AAATCTGAAA TTAAAAAGTT ATCTCAGGGA 
TCTCCAGAAC CGATTGAGCC CAACTTTTTC ACAGCAGATT ACCATTTATT ACATCGTTCA 
TCCGGTGGAA ACAGCCTGTC CCCAAATGAC CCTACAGGTT TACCAACCAG CATTGAATTG 
GAGGAAGGAA TAACATATGA ACAGATGCAG ACTGTGATTG AAGAAGTCCT TGAGGAAAGT 
GGCTATTACA ATTTTACATC TAACAGGTAT CATTCCTATC CATGGGGGAC CAAGAATCAC 
CCAACCAAAA GATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001291313.1
CDS	278..1732
Translation	MVPVQPAEPPEVLKSEPYGEKADVWAVGCILYQMATLSPPFYSTNMLSLATKIVEAVYEPVPEGIYSEKVTDTISRCLTPDAEARPDIVEVSSMISDVMMKYLDNLSTSQLSLEKKLERERRRTQRYFMEANRNTVTCHHELAVLSHETFEKASLSSSSSGAASLKSELSESADLPPEGFQASYGKDEDRACDEILSDDNFNLENAEKDTYSEVDDELDISDNSSSSSSSPLKESTFNILKRSFSASGGERQSQTRDFTGGTGSRPRPALLPLDLLLKVPPHMLRAHIKEIEAELVTGWQSHSLPAVILRNLKDHGPQMGTFLWQASAGIAVSQRKVRQISDPIQQILIQLHKIIYITQLPPALHHNLKRRVIERFKKSLFSQQSNPCNLKSEIKKLSQGSPEPIEPNFFTADYHLLHRSSGGNSLSPNDPTGLPTSIELEEGITYEQMQTVIEEVLEESGYYNFTSNRYHSYPWGTKNHPTKR

Target ORF information:

RefSeq Version	NM_001304384.1
Organism	Homo sapiens(human)
Definition	Homo sapiens NIMA related kinase 10 (NEK10), transcript variant 4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001304384.1

ORF Insert Sequence:

ATGGTTCCTG TACAGCCTGC AGAACCCCCC GAGGTACTGA AGAGTGAGCC GTATGGGGAG 
AAGGCTGATG TCTGGGCAGT AGGCTGCATC CTTTATCAGA TGGCGACTTT GAGTCCCCCC 
TTCTACAGCA CTAACATGCT GTCCTTGGCT ACAAAAATAG TGGAGGCGGT ATATGAACCA 
GTCCCAGAAG GTATCTACTC TGAAAAAGTA ACAGACACCA TCAGCAGGTG CCTCACTCCT 
GATGCGGAAG CTCGTCCAGA TATTGTAGAA GTCAGTTCGA TGATATCAGA TGTCATGATG 
AAATATTTAG ACAACTTATC TACATCCCAG TTGTCCTTGG AAAAGAAGCT AGAACGGGAA 
CGAAGACGCA CACAAAGGTA TTTTATGGAA GCCAACCGGA ACACCGTCAC ATGTCACCAT 
GAGCTGGCTG TTCTATCTCA CGAGACCTTT GAGAAGGCAA GTTTGAGTAG CAGCAGCAGT 
GGAGCAGCCA GCCTGAAAAG TGAACTTTCA GAAAGCGCAG ACCTGCCCCC TGAAGGCTTC 
CAGGCCTCCT ATGGTAAAGA CGAAGACAGG GCCTGTGACG AAATCCTGTC AGATGATAAC 
TTCAACCTGG AAAATGCTGA GAAAGATACA TATTCAGAGG TAGATGATGA ATTGGACATT 
TCGGATAACT CCAGCAGCTC CAGTTCAAGC CCTCTGAAAG AATCTACATT CAACATTTTA 
AAGAGAAGTT TTAGTGCTTC AGGAGGAGAA AGACAATCCC AAACAAGGGA CTTCACTGGA 
GGAACAGGAT CAAGACCAAG ACCAGCTTTG CTGCCTCTTG ACCTGCTTCT GAAAGTGCCA 
CCCCACATGC TCAGGGCCCA CATTAAGGAA ATAGAGGCTG AGTTAGTGAC AGGGTGGCAG 
TCCCATAGCC TTCCTGCTGT GATTCTTCGA AATCTCAAAG ATCATGGGCC ACAGATGGGC 
ACATTCTTGT GGCAAGCATC AGCAGGAATT GCTGTGTCCC AGAGGAAAGT GCGTCAGATC 
AGTGATCCTA TTCAGCAGAT ATTAATTCAG CTGCACAAAA TAATCTATAT CACACAGCTT 
CCTCCAGCTT TGCACCACAA TTTGAAAAGA AGGGTTATAG AGAGATTCAA GAAATCCCTC 
TTCAGCCAGC AGAGTAACCC TTGTAATTTG AAATCTGAAA TTAAAAAGTT ATCTCAGGGA 
TCTCCAGAAC CGATTGAGCC CAACTTTTTC ACAGCAGATT ACCATTTATT ACATCGTTCA 
TCCGGTGGAA ACAGCCTGTC CCCAAATGAC CCTACAGGTT TACCAACCAG CATTGAATTG 
GAGGAAGGAA TAACATATGA ACAGATGCAG ACTGTGATTG AAGAAGTCCT TGAGGAAAGT 
GGCTATTACA ATTTTACATC TAACAGGTAT CATTCCTATC CATGGGGGAC CAAGAATCAC 
CCAACCAAAA GATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NEK10 ( XM_011533415.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_011531717.1 Homo sapiens NIMA related kinase 10 (NEK10), transcript variant X25, mRNA.

Price & Availability↑

CloneID	OHu76709
Clone ID Related Accession (Same CDS sequence)	XM_011533415.1
Accession Version	XM_011533415.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1992bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	serine/threonine-protein kinase Nek10 isoform X15
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000003.12) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCTGATC AAGATAAAAA GGTGAAGACC ACAGAAAAAT CAACTGATAA ACAGCAAGAA 
ATCACCATCA GGGACTATTC AGATCTTAAA AGACTTCGGT GCCTTTTGAA CGTCCAATCA 
AGCAAACAAC AGCTTCCAGC CATTAACTTC GATAGTGCCC AAAATAGCAT GACGAAGTCT 
GAGCCCGCCA TCAGGGCGGG TGGACACAGA GCTCGGGGTC AGTGGCATGA ATCCACAGAA 
GCTGTTGAAC TTGAAAATTT TAGTATAAAC TACAAGAATG AGAGAAATTT CAGCAAACAT 
CCTCAGCGTA AACTATTTCA GGAGATCTTT ACCGCCTTGG TGAAAAATAG ACTCATAAGC 
AGAGAGTGGG TTAATCGAGC CCCATCTATT CATTTTCTGA GAGTGTTAAT CTGTCTGAGG 
CTACTAATGA GGGATCCATG TTATCAGGAA ATACTCCATA GCTTGGGTGG GATTGAAAAC 
CTAGCTCAGT ATATGGAGAT TGTAGCCAAT GAGTACCTCG GCTATGGAGA AGAGCAGCAC 
ACTGTGGACA AGCTGGTCAA CATGACATAT ATTTTTCAAA AACTTGCTGC AGTCAAAGAT 
CAAAGAGAAT GGGTCACCAC AAGTGGAGCC CACAAGACAT TAGTAAATTT ACTTGGTGCC 
CGAGATACTA ATGTTCTATT GGGTTCCCTT CTGGCTCTGG CTAGTTTAGC AGAAAGTCAA 
GAATGTAGGG AGAAGATAAG TGAACTCAAC ATTGTAGAAA ATCTGTTGAT GATTTTACAT 
GAATATGACT TGCTTTCTAA AAGACTAACA GCGGAGTTGC TGCGCCTACT TTGTGCAGAG 
CCCCAGGTGA AAGAGCAGGT GAAGCTCTAT GAGGGGATAC CGGTCCTCCT CAGTCTGCTC 
CACTCTGACC ACTTGAAGCT CCTCTGGAGC ATTGTCTGGA TTCTGGTACA GGTTTGTGAG 
GACCCTGAGA CCAGCGTGGA AATTCGCATT TGGGGAGGCA TCAAACAGCT TCTTCATATT 
TTACAAGGAG ACAGAAATTT TGTTTCTGAT CACTCCTCCA TTGGAAGCCT GTCCAGTGCA 
AATGCTGCAG GCCGAATCCA GCAGCTTCAT TTATCAGAAG ACTTGAGCCC TAGGGAAATA 
CAAGAAAATA CTTTCTCACT TCAAGCAGCC TGCTGTGCTG CCCTCACTGA GCTGGTGCTC 
AATGACACCA ATGCCCACCA GGTGGTTCAG GAAAATGGTG TATATACAAT AGCAAAATTA 
ATTTTACCAA ATAAGCAAAA GAATGCAGCA AAAAGTAATC TATTACAGTG TTATGCTTTC 
AGAGCCTTGA GATTTCTCTT CAGTATGGAA AGAAACAGAC CACTCTTTAA AAGACTTTTC 
CCCACAGACT TGTTTGAGAT CTTCATTGAC ATAGGGCATT ATGTACGTGA TATCAGTGCT 
TATGAAGAAT TGGTATCCAA GCTGAATTTA TTAGTGGAGG ATGAACTGAA GCAAATTGCT 
GAAAATATTG AAAGCATTAA TCAGAACAAA GCTCCTTTGA AATATATAGG CAACTATGCA 
ATTTTGGATC ATCTTGGAAG TGGAGCTTTT GGCTGTGTTT ACAAGCTTTA TCATCCCAAC 
ATTGTACGTT ATTACAAAAC ATTTCTGGAA AATGATAGGT TGTACATAGT TATGGAGCTG 
ATAGAAGGAG CCCCGCTTGG AGAGCATTTC AGTTCTTTGA AGGAAAAACA TCACCATTTT 
ACTGAAGAAA GACTATGGAA AATATTTATA CAGCTGTGCT TAGCTCTTCG ATACTTACAC 
AAGGAGAAGA GGATTGTCCA TAGAGATCTG ACACCAAACA ACATTATGTT GGGGGATAAG 
GACAAAGTAA CCGTTACTGA CTTTGGCCTG GCAAAGCAAA AACAAGAAAA CAGTAAACTC 
ACCTCTGTGG TTGGAACAAT CCTGTATTCT TGTGGTTTCA TGAGAAGATG CAAAGAAACT 
GTGGCAGGCT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011531717.1
CDS	275..2266
Translation	MPDQDKKVKTTEKSTDKQQEITIRDYSDLKRLRCLLNVQSSKQQLPAINFDSAQNSMTKSEPAIRAGGHRARGQWHESTEAVELENFSINYKNERNFSKHPQRKLFQEIFTALVKNRLISREWVNRAPSIHFLRVLICLRLLMRDPCYQEILHSLGGIENLAQYMEIVANEYLGYGEEQHTVDKLVNMTYIFQKLAAVKDQREWVTTSGAHKTLVNLLGARDTNVLLGSLLALASLAESQECREKISELNIVENLLMILHEYDLLSKRLTAELLRLLCAEPQVKEQVKLYEGIPVLLSLLHSDHLKLLWSIVWILVQVCEDPETSVEIRIWGGIKQLLHILQGDRNFVSDHSSIGSLSSANAAGRIQQLHLSEDLSPREIQENTFSLQAACCAALTELVLNDTNAHQVVQENGVYTIAKLILPNKQKNAAKSNLLQCYAFRALRFLFSMERNRPLFKRLFPTDLFEIFIDIGHYVRDISAYEELVSKLNLLVEDELKQIAENIESINQNKAPLKYIGNYAILDHLGSGAFGCVYKLYHPNIVRYYKTFLENDRLYIVMELIEGAPLGEHFSSLKEKHHHFTEERLWKIFIQLCLALRYLHKEKRIVHRDLTPNNIMLGDKDKVTVTDFGLAKQKQENSKLTSVVGTILYSCGFMRRCKETVAG

Target ORF information:

RefSeq Version	XM_011533415.1
Organism	Homo sapiens(human)
Definition	Homo sapiens NIMA related kinase 10 (NEK10), transcript variant X25, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011533415.1

ORF Insert Sequence:

ATGCCTGATC AAGATAAAAA GGTGAAGACC ACAGAAAAAT CAACTGATAA ACAGCAAGAA 
ATCACCATCA GGGACTATTC AGATCTTAAA AGACTTCGGT GCCTTTTGAA CGTCCAATCA 
AGCAAACAAC AGCTTCCAGC CATTAACTTC GATAGTGCCC AAAATAGCAT GACGAAGTCT 
GAGCCCGCCA TCAGGGCGGG TGGACACAGA GCTCGGGGTC AGTGGCATGA ATCCACAGAA 
GCTGTTGAAC TTGAAAATTT TAGTATAAAC TACAAGAATG AGAGAAATTT CAGCAAACAT 
CCTCAGCGTA AACTATTTCA GGAGATCTTT ACCGCCTTGG TGAAAAATAG ACTCATAAGC 
AGAGAGTGGG TTAATCGAGC CCCATCTATT CATTTTCTGA GAGTGTTAAT CTGTCTGAGG 
CTACTAATGA GGGATCCATG TTATCAGGAA ATACTCCATA GCTTGGGTGG GATTGAAAAC 
CTAGCTCAGT ATATGGAGAT TGTAGCCAAT GAGTACCTCG GCTATGGAGA AGAGCAGCAC 
ACTGTGGACA AGCTGGTCAA CATGACATAT ATTTTTCAAA AACTTGCTGC AGTCAAAGAT 
CAAAGAGAAT GGGTCACCAC AAGTGGAGCC CACAAGACAT TAGTAAATTT ACTTGGTGCC 
CGAGATACTA ATGTTCTATT GGGTTCCCTT CTGGCTCTGG CTAGTTTAGC AGAAAGTCAA 
GAATGTAGGG AGAAGATAAG TGAACTCAAC ATTGTAGAAA ATCTGTTGAT GATTTTACAT 
GAATATGACT TGCTTTCTAA AAGACTAACA GCGGAGTTGC TGCGCCTACT TTGTGCAGAG 
CCCCAGGTGA AAGAGCAGGT GAAGCTCTAT GAGGGGATAC CGGTCCTCCT CAGTCTGCTC 
CACTCTGACC ACTTGAAGCT CCTCTGGAGC ATTGTCTGGA TTCTGGTACA GGTTTGTGAG 
GACCCTGAGA CCAGCGTGGA AATTCGCATT TGGGGAGGCA TCAAACAGCT TCTTCATATT 
TTACAAGGAG ACAGAAATTT TGTTTCTGAT CACTCCTCCA TTGGAAGCCT GTCCAGTGCA 
AATGCTGCAG GCCGAATCCA GCAGCTTCAT TTATCAGAAG ACTTGAGCCC TAGGGAAATA 
CAAGAAAATA CTTTCTCACT TCAAGCAGCC TGCTGTGCTG CCCTCACTGA GCTGGTGCTC 
AATGACACCA ATGCCCACCA GGTGGTTCAG GAAAATGGTG TATATACAAT AGCAAAATTA 
ATTTTACCAA ATAAGCAAAA GAATGCAGCA AAAAGTAATC TATTACAGTG TTATGCTTTC 
AGAGCCTTGA GATTTCTCTT CAGTATGGAA AGAAACAGAC CACTCTTTAA AAGACTTTTC 
CCCACAGACT TGTTTGAGAT CTTCATTGAC ATAGGGCATT ATGTACGTGA TATCAGTGCT 
TATGAAGAAT TGGTATCCAA GCTGAATTTA TTAGTGGAGG ATGAACTGAA GCAAATTGCT 
GAAAATATTG AAAGCATTAA TCAGAACAAA GCTCCTTTGA AATATATAGG CAACTATGCA 
ATTTTGGATC ATCTTGGAAG TGGAGCTTTT GGCTGTGTTT ACAAGCTTTA TCATCCCAAC 
ATTGTACGTT ATTACAAAAC ATTTCTGGAA AATGATAGGT TGTACATAGT TATGGAGCTG 
ATAGAAGGAG CCCCGCTTGG AGAGCATTTC AGTTCTTTGA AGGAAAAACA TCACCATTTT 
ACTGAAGAAA GACTATGGAA AATATTTATA CAGCTGTGCT TAGCTCTTCG ATACTTACAC 
AAGGAGAAGA GGATTGTCCA TAGAGATCTG ACACCAAACA ACATTATGTT GGGGGATAAG 
GACAAAGTAA CCGTTACTGA CTTTGGCCTG GCAAAGCAAA AACAAGAAAA CAGTAAACTC 
ACCTCTGTGG TTGGAACAAT CCTGTATTCT TGTGGTTTCA TGAGAAGATG CAAAGAAACT 
GTGGCAGGCT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NEK10 ( XM_017005774.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_016861263.1 Homo sapiens NIMA related kinase 10 (NEK10), transcript variant X21, mRNA.

Price & Availability↑

CloneID	OHu36997
Clone ID Related Accession (Same CDS sequence)	XM_017005774.2 , XM_017005774.1 , XM_011533413.2
Accession Version	XM_017005774.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2142bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	serine/threonine-protein kinase Nek10 isoform X11
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000003.12) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_017005774.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCTGATC AAGATAAAAA GGTGAAGACC ACAGAAAAAT CAACTGATAA ACAGCAAGAA 
ATCACCATCA GGGACTATTC AGATCTTAAA AGACTTCGGT GCCTTTTGAA CGTCCAATCA 
AGCAAACAAC AGCTTCCAGC CATTAACTTC GATAGTGCCC AAAATAGCAT GACGAAGTCT 
GAGCCCGCCA TCAGGGCGGG TGGACACAGA GCTCGGGGTC AGTGGCATGA ATCCACAGAA 
GCTGTTGAAC TTGAAAATTT TAGTATAAAC TACAAGAATG AGAGAAATTT CAGCAAACAT 
CCTCAGCGTA AACTATTTCA GGAGATCTTT ACCGCCTTGG TGAAAAATAG ACTCATAAGC 
AGAGAGTGGG TTAATCGAGC CCCATCTATT CATTTTCTGA GAGTGTTAAT CTGTCTGAGG 
CTACTAATGA GGGATCCATG TTATCAGGAA ATACTCCATA GCTTGGGTGG GATTGAAAAC 
CTAGCTCAGT ATATGGAGAT TGTAGCCAAT GAGTACCTCG GCTATGGAGA AGAGCAGCAC 
ACTGTGGACA AGCTGGTCAA CATGACATAT ATTTTTCAAA AACTTGCTGC AGTCAAAGAT 
CAAAGAGAAT GGGTCACCAC AAGTGGAGCC CACAAGACAT TAGTAAATTT ACTTGGTGCC 
CGAGATACTA ATGTTCTATT GGGTTCCCTT CTGGCTCTGG CTAGTTTAGC AGAAAGTCAA 
GAATGTAGGG AGAAGATAAG TGAACTCAAC ATTGTAGAAA ATCTGTTGAT GATTTTACAT 
GAATATGACT TGCTTTCTAA AAGACTAACA GCGGAGTTGC TGCGCCTACT TTGTGCAGAG 
CCCCAGGTGA AAGAGCAGGT GAAGCTCTAT GAGGGGATAC CGGTCCTCCT CAGTCTGCTC 
CACTCTGACC ACTTGAAGCT CCTCTGGAGC ATTGTCTGGA TTCTGGTACA GGTTTGTGAG 
GACCCTGAGA CCAGCGTGGA AATTCGCATT TGGGGAGGCA TCAAACAGCT TCTTCATATT 
TTACAAGGAG ACAGAAATTT TGTTTCTGAT CACTCCTCCA TTGGAAGCCT GTCCAGTGCA 
AATGCTGCAG GCCGAATCCA GCAGCTTCAT TTATCAGAAG ACTTGAGCCC TAGGGAAATA 
CAAGAAAATA CTTTCTCACT TCAAGCAGCC TGCTGTGCTG CCCTCACTGA GCTGGTGCTC 
AATGACACCA ATGCCCACCA GGTGGTTCAG GAAAATGGTG TATATACAAT AGCAAAATTA 
ATTTTACCAA ATAAGCAAAA GAATGCAGCA AAAAGTAATC TATTACAGTG TTATGCTTTC 
AGAGCCTTGA GATTTCTCTT CAGTATGGAA AGAAACAGAC CACTCTTTAA AAGACTTTTC 
CCCACAGACT TGTTTGAGAT CTTCATTGAC ATAGGGCATT ATGTACGTGA TATCAGTGCT 
TATGAAGAAT TGGTATCCAA GCTGAATTTA TTAGTGGAGG ATGAACTGAA GCAAATTGCT 
GAAAATATTG AAAGCATTAA TCAGAACAAA GCTCCTTTGA AATATATAGG CAACTATGCA 
ATTTTGGATC ATCTTGGAAG TGGAGCTTTT GGCTGTGTTT ACAAGGTTAG AAAGCATAGT 
GGTCAAAATC TTTTAGCAAT GAAAGAGGTC AATTTACATA ACCCAGCATT TGGAAAGGAT 
AAGAAAGATC GAGACAGCAG CGTAAGGAAT ATTGTTTCTG AATTAACAAT AATTAAAGAG 
CAGCTTTATC ATCCCAACAT TGTACGTTAT TACAAAACAT TTCTGGAAAA TGATAGGTTG 
TACATAGTTA TGGAGCTGAT AGAAGGAGCC CCGCTTGGAG AGCATTTCAG TTCTTTGAAG 
GAAAAACATC ACCATTTTAC TGAAGAAAGA CTATGGAAAA TATTTATACA GCTGTGCTTA 
GCTCTTCGAT ACTTACACAA GGAGAAGAGG ATTGTCCATA GAGATCTGAC ACCAAACAAC 
ATTATGTTGG GGGATAAGGA CAAAGTAACC GTTACCTTTG AAACCATCCA AGGAAACAGA 
CCCCGATTCA GTGGTACATG GGGTGAGACA CAGTCAACAT TTGCCCTAAA TACTGTCACT 
TGCCAAATAA GGTCCATATT GTGCCATCAC ATTACAAAAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016861263.1
CDS	274..2415
Translation	MPDQDKKVKTTEKSTDKQQEITIRDYSDLKRLRCLLNVQSSKQQLPAINFDSAQNSMTKSEPAIRAGGHRARGQWHESTEAVELENFSINYKNERNFSKHPQRKLFQEIFTALVKNRLISREWVNRAPSIHFLRVLICLRLLMRDPCYQEILHSLGGIENLAQYMEIVANEYLGYGEEQHTVDKLVNMTYIFQKLAAVKDQREWVTTSGAHKTLVNLLGARDTNVLLGSLLALASLAESQECREKISELNIVENLLMILHEYDLLSKRLTAELLRLLCAEPQVKEQVKLYEGIPVLLSLLHSDHLKLLWSIVWILVQVCEDPETSVEIRIWGGIKQLLHILQGDRNFVSDHSSIGSLSSANAAGRIQQLHLSEDLSPREIQENTFSLQAACCAALTELVLNDTNAHQVVQENGVYTIAKLILPNKQKNAAKSNLLQCYAFRALRFLFSMERNRPLFKRLFPTDLFEIFIDIGHYVRDISAYEELVSKLNLLVEDELKQIAENIESINQNKAPLKYIGNYAILDHLGSGAFGCVYKVRKHSGQNLLAMKEVNLHNPAFGKDKKDRDSSVRNIVSELTIIKEQLYHPNIVRYYKTFLENDRLYIVMELIEGAPLGEHFSSLKEKHHHFTEERLWKIFIQLCLALRYLHKEKRIVHRDLTPNNIMLGDKDKVTVTFETIQGNRPRFSGTWGETQSTFALNTVTCQIRSILCHHITK

Target ORF information:

RefSeq Version	XM_017005774.2
Organism	Homo sapiens(human)
Definition	Homo sapiens NIMA related kinase 10 (NEK10), transcript variant X21, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017005774.2

ORF Insert Sequence:

ATGCCTGATC AAGATAAAAA GGTGAAGACC ACAGAAAAAT CAACTGATAA ACAGCAAGAA 
ATCACCATCA GGGACTATTC AGATCTTAAA AGACTTCGGT GCCTTTTGAA CGTCCAATCA 
AGCAAACAAC AGCTTCCAGC CATTAACTTC GATAGTGCCC AAAATAGCAT GACGAAGTCT 
GAGCCCGCCA TCAGGGCGGG TGGACACAGA GCTCGGGGTC AGTGGCATGA ATCCACAGAA 
GCTGTTGAAC TTGAAAATTT TAGTATAAAC TACAAGAATG AGAGAAATTT CAGCAAACAT 
CCTCAGCGTA AACTATTTCA GGAGATCTTT ACCGCCTTGG TGAAAAATAG ACTCATAAGC 
AGAGAGTGGG TTAATCGAGC CCCATCTATT CATTTTCTGA GAGTGTTAAT CTGTCTGAGG 
CTACTAATGA GGGATCCATG TTATCAGGAA ATACTCCATA GCTTGGGTGG GATTGAAAAC 
CTAGCTCAGT ATATGGAGAT TGTAGCCAAT GAGTACCTCG GCTATGGAGA AGAGCAGCAC 
ACTGTGGACA AGCTGGTCAA CATGACATAT ATTTTTCAAA AACTTGCTGC AGTCAAAGAT 
CAAAGAGAAT GGGTCACCAC AAGTGGAGCC CACAAGACAT TAGTAAATTT ACTTGGTGCC 
CGAGATACTA ATGTTCTATT GGGTTCCCTT CTGGCTCTGG CTAGTTTAGC AGAAAGTCAA 
GAATGTAGGG AGAAGATAAG TGAACTCAAC ATTGTAGAAA ATCTGTTGAT GATTTTACAT 
GAATATGACT TGCTTTCTAA AAGACTAACA GCGGAGTTGC TGCGCCTACT TTGTGCAGAG 
CCCCAGGTGA AAGAGCAGGT GAAGCTCTAT GAGGGGATAC CGGTCCTCCT CAGTCTGCTC 
CACTCTGACC ACTTGAAGCT CCTCTGGAGC ATTGTCTGGA TTCTGGTACA GGTTTGTGAG 
GACCCTGAGA CCAGCGTGGA AATTCGCATT TGGGGAGGCA TCAAACAGCT TCTTCATATT 
TTACAAGGAG ACAGAAATTT TGTTTCTGAT CACTCCTCCA TTGGAAGCCT GTCCAGTGCA 
AATGCTGCAG GCCGAATCCA GCAGCTTCAT TTATCAGAAG ACTTGAGCCC TAGGGAAATA 
CAAGAAAATA CTTTCTCACT TCAAGCAGCC TGCTGTGCTG CCCTCACTGA GCTGGTGCTC 
AATGACACCA ATGCCCACCA GGTGGTTCAG GAAAATGGTG TATATACAAT AGCAAAATTA 
ATTTTACCAA ATAAGCAAAA GAATGCAGCA AAAAGTAATC TATTACAGTG TTATGCTTTC 
AGAGCCTTGA GATTTCTCTT CAGTATGGAA AGAAACAGAC CACTCTTTAA AAGACTTTTC 
CCCACAGACT TGTTTGAGAT CTTCATTGAC ATAGGGCATT ATGTACGTGA TATCAGTGCT 
TATGAAGAAT TGGTATCCAA GCTGAATTTA TTAGTGGAGG ATGAACTGAA GCAAATTGCT 
GAAAATATTG AAAGCATTAA TCAGAACAAA GCTCCTTTGA AATATATAGG CAACTATGCA 
ATTTTGGATC ATCTTGGAAG TGGAGCTTTT GGCTGTGTTT ACAAGGTTAG AAAGCATAGT 
GGTCAAAATC TTTTAGCAAT GAAAGAGGTC AATTTACATA ACCCAGCATT TGGAAAGGAT 
AAGAAAGATC GAGACAGCAG CGTAAGGAAT ATTGTTTCTG AATTAACAAT AATTAAAGAG 
CAGCTTTATC ATCCCAACAT TGTACGTTAT TACAAAACAT TTCTGGAAAA TGATAGGTTG 
TACATAGTTA TGGAGCTGAT AGAAGGAGCC CCGCTTGGAG AGCATTTCAG TTCTTTGAAG 
GAAAAACATC ACCATTTTAC TGAAGAAAGA CTATGGAAAA TATTTATACA GCTGTGCTTA 
GCTCTTCGAT ACTTACACAA GGAGAAGAGG ATTGTCCATA GAGATCTGAC ACCAAACAAC 
ATTATGTTGG GGGATAAGGA CAAAGTAACC GTTACCTTTG AAACCATCCA AGGAAACAGA 
CCCCGATTCA GTGGTACATG GGGTGAGACA CAGTCAACAT TTGCCCTAAA TACTGTCACT 
TGCCAAATAA GGTCCATATT GTGCCATCAC ATTACAAAAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NEK10 ( XM_017005774.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016861263.1 Homo sapiens NIMA related kinase 10 (NEK10), transcript variant X20, mRNA.

Price & Availability↑

CloneID	OHu36997
Clone ID Related Accession (Same CDS sequence)	XM_017005774.2 , XM_017005774.1 , XM_011533413.2
Accession Version	XM_017005774.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2142bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	serine/threonine-protein kinase Nek10 isoform X11
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000003.12) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCTGATC AAGATAAAAA GGTGAAGACC ACAGAAAAAT CAACTGATAA ACAGCAAGAA 
ATCACCATCA GGGACTATTC AGATCTTAAA AGACTTCGGT GCCTTTTGAA CGTCCAATCA 
AGCAAACAAC AGCTTCCAGC CATTAACTTC GATAGTGCCC AAAATAGCAT GACGAAGTCT 
GAGCCCGCCA TCAGGGCGGG TGGACACAGA GCTCGGGGTC AGTGGCATGA ATCCACAGAA 
GCTGTTGAAC TTGAAAATTT TAGTATAAAC TACAAGAATG AGAGAAATTT CAGCAAACAT 
CCTCAGCGTA AACTATTTCA GGAGATCTTT ACCGCCTTGG TGAAAAATAG ACTCATAAGC 
AGAGAGTGGG TTAATCGAGC CCCATCTATT CATTTTCTGA GAGTGTTAAT CTGTCTGAGG 
CTACTAATGA GGGATCCATG TTATCAGGAA ATACTCCATA GCTTGGGTGG GATTGAAAAC 
CTAGCTCAGT ATATGGAGAT TGTAGCCAAT GAGTACCTCG GCTATGGAGA AGAGCAGCAC 
ACTGTGGACA AGCTGGTCAA CATGACATAT ATTTTTCAAA AACTTGCTGC AGTCAAAGAT 
CAAAGAGAAT GGGTCACCAC AAGTGGAGCC CACAAGACAT TAGTAAATTT ACTTGGTGCC 
CGAGATACTA ATGTTCTATT GGGTTCCCTT CTGGCTCTGG CTAGTTTAGC AGAAAGTCAA 
GAATGTAGGG AGAAGATAAG TGAACTCAAC ATTGTAGAAA ATCTGTTGAT GATTTTACAT 
GAATATGACT TGCTTTCTAA AAGACTAACA GCGGAGTTGC TGCGCCTACT TTGTGCAGAG 
CCCCAGGTGA AAGAGCAGGT GAAGCTCTAT GAGGGGATAC CGGTCCTCCT CAGTCTGCTC 
CACTCTGACC ACTTGAAGCT CCTCTGGAGC ATTGTCTGGA TTCTGGTACA GGTTTGTGAG 
GACCCTGAGA CCAGCGTGGA AATTCGCATT TGGGGAGGCA TCAAACAGCT TCTTCATATT 
TTACAAGGAG ACAGAAATTT TGTTTCTGAT CACTCCTCCA TTGGAAGCCT GTCCAGTGCA 
AATGCTGCAG GCCGAATCCA GCAGCTTCAT TTATCAGAAG ACTTGAGCCC TAGGGAAATA 
CAAGAAAATA CTTTCTCACT TCAAGCAGCC TGCTGTGCTG CCCTCACTGA GCTGGTGCTC 
AATGACACCA ATGCCCACCA GGTGGTTCAG GAAAATGGTG TATATACAAT AGCAAAATTA 
ATTTTACCAA ATAAGCAAAA GAATGCAGCA AAAAGTAATC TATTACAGTG TTATGCTTTC 
AGAGCCTTGA GATTTCTCTT CAGTATGGAA AGAAACAGAC CACTCTTTAA AAGACTTTTC 
CCCACAGACT TGTTTGAGAT CTTCATTGAC ATAGGGCATT ATGTACGTGA TATCAGTGCT 
TATGAAGAAT TGGTATCCAA GCTGAATTTA TTAGTGGAGG ATGAACTGAA GCAAATTGCT 
GAAAATATTG AAAGCATTAA TCAGAACAAA GCTCCTTTGA AATATATAGG CAACTATGCA 
ATTTTGGATC ATCTTGGAAG TGGAGCTTTT GGCTGTGTTT ACAAGGTTAG AAAGCATAGT 
GGTCAAAATC TTTTAGCAAT GAAAGAGGTC AATTTACATA ACCCAGCATT TGGAAAGGAT 
AAGAAAGATC GAGACAGCAG CGTAAGGAAT ATTGTTTCTG AATTAACAAT AATTAAAGAG 
CAGCTTTATC ATCCCAACAT TGTACGTTAT TACAAAACAT TTCTGGAAAA TGATAGGTTG 
TACATAGTTA TGGAGCTGAT AGAAGGAGCC CCGCTTGGAG AGCATTTCAG TTCTTTGAAG 
GAAAAACATC ACCATTTTAC TGAAGAAAGA CTATGGAAAA TATTTATACA GCTGTGCTTA 
GCTCTTCGAT ACTTACACAA GGAGAAGAGG ATTGTCCATA GAGATCTGAC ACCAAACAAC 
ATTATGTTGG GGGATAAGGA CAAAGTAACC GTTACCTTTG AAACCATCCA AGGAAACAGA 
CCCCGATTCA GTGGTACATG GGGTGAGACA CAGTCAACAT TTGCCCTAAA TACTGTCACT 
TGCCAAATAA GGTCCATATT GTGCCATCAC ATTACAAAAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016861263.1
CDS	227..2368
Translation	MPDQDKKVKTTEKSTDKQQEITIRDYSDLKRLRCLLNVQSSKQQLPAINFDSAQNSMTKSEPAIRAGGHRARGQWHESTEAVELENFSINYKNERNFSKHPQRKLFQEIFTALVKNRLISREWVNRAPSIHFLRVLICLRLLMRDPCYQEILHSLGGIENLAQYMEIVANEYLGYGEEQHTVDKLVNMTYIFQKLAAVKDQREWVTTSGAHKTLVNLLGARDTNVLLGSLLALASLAESQECREKISELNIVENLLMILHEYDLLSKRLTAELLRLLCAEPQVKEQVKLYEGIPVLLSLLHSDHLKLLWSIVWILVQVCEDPETSVEIRIWGGIKQLLHILQGDRNFVSDHSSIGSLSSANAAGRIQQLHLSEDLSPREIQENTFSLQAACCAALTELVLNDTNAHQVVQENGVYTIAKLILPNKQKNAAKSNLLQCYAFRALRFLFSMERNRPLFKRLFPTDLFEIFIDIGHYVRDISAYEELVSKLNLLVEDELKQIAENIESINQNKAPLKYIGNYAILDHLGSGAFGCVYKVRKHSGQNLLAMKEVNLHNPAFGKDKKDRDSSVRNIVSELTIIKEQLYHPNIVRYYKTFLENDRLYIVMELIEGAPLGEHFSSLKEKHHHFTEERLWKIFIQLCLALRYLHKEKRIVHRDLTPNNIMLGDKDKVTVTFETIQGNRPRFSGTWGETQSTFALNTVTCQIRSILCHHITK

Target ORF information:

RefSeq Version	XM_017005774.1
Organism	Homo sapiens(human)
Definition	Homo sapiens NIMA related kinase 10 (NEK10), transcript variant X20, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017005774.1

ORF Insert Sequence:

ATGCCTGATC AAGATAAAAA GGTGAAGACC ACAGAAAAAT CAACTGATAA ACAGCAAGAA 
ATCACCATCA GGGACTATTC AGATCTTAAA AGACTTCGGT GCCTTTTGAA CGTCCAATCA 
AGCAAACAAC AGCTTCCAGC CATTAACTTC GATAGTGCCC AAAATAGCAT GACGAAGTCT 
GAGCCCGCCA TCAGGGCGGG TGGACACAGA GCTCGGGGTC AGTGGCATGA ATCCACAGAA 
GCTGTTGAAC TTGAAAATTT TAGTATAAAC TACAAGAATG AGAGAAATTT CAGCAAACAT 
CCTCAGCGTA AACTATTTCA GGAGATCTTT ACCGCCTTGG TGAAAAATAG ACTCATAAGC 
AGAGAGTGGG TTAATCGAGC CCCATCTATT CATTTTCTGA GAGTGTTAAT CTGTCTGAGG 
CTACTAATGA GGGATCCATG TTATCAGGAA ATACTCCATA GCTTGGGTGG GATTGAAAAC 
CTAGCTCAGT ATATGGAGAT TGTAGCCAAT GAGTACCTCG GCTATGGAGA AGAGCAGCAC 
ACTGTGGACA AGCTGGTCAA CATGACATAT ATTTTTCAAA AACTTGCTGC AGTCAAAGAT 
CAAAGAGAAT GGGTCACCAC AAGTGGAGCC CACAAGACAT TAGTAAATTT ACTTGGTGCC 
CGAGATACTA ATGTTCTATT GGGTTCCCTT CTGGCTCTGG CTAGTTTAGC AGAAAGTCAA 
GAATGTAGGG AGAAGATAAG TGAACTCAAC ATTGTAGAAA ATCTGTTGAT GATTTTACAT 
GAATATGACT TGCTTTCTAA AAGACTAACA GCGGAGTTGC TGCGCCTACT TTGTGCAGAG 
CCCCAGGTGA AAGAGCAGGT GAAGCTCTAT GAGGGGATAC CGGTCCTCCT CAGTCTGCTC 
CACTCTGACC ACTTGAAGCT CCTCTGGAGC ATTGTCTGGA TTCTGGTACA GGTTTGTGAG 
GACCCTGAGA CCAGCGTGGA AATTCGCATT TGGGGAGGCA TCAAACAGCT TCTTCATATT 
TTACAAGGAG ACAGAAATTT TGTTTCTGAT CACTCCTCCA TTGGAAGCCT GTCCAGTGCA 
AATGCTGCAG GCCGAATCCA GCAGCTTCAT TTATCAGAAG ACTTGAGCCC TAGGGAAATA 
CAAGAAAATA CTTTCTCACT TCAAGCAGCC TGCTGTGCTG CCCTCACTGA GCTGGTGCTC 
AATGACACCA ATGCCCACCA GGTGGTTCAG GAAAATGGTG TATATACAAT AGCAAAATTA 
ATTTTACCAA ATAAGCAAAA GAATGCAGCA AAAAGTAATC TATTACAGTG TTATGCTTTC 
AGAGCCTTGA GATTTCTCTT CAGTATGGAA AGAAACAGAC CACTCTTTAA AAGACTTTTC 
CCCACAGACT TGTTTGAGAT CTTCATTGAC ATAGGGCATT ATGTACGTGA TATCAGTGCT 
TATGAAGAAT TGGTATCCAA GCTGAATTTA TTAGTGGAGG ATGAACTGAA GCAAATTGCT 
GAAAATATTG AAAGCATTAA TCAGAACAAA GCTCCTTTGA AATATATAGG CAACTATGCA 
ATTTTGGATC ATCTTGGAAG TGGAGCTTTT GGCTGTGTTT ACAAGGTTAG AAAGCATAGT 
GGTCAAAATC TTTTAGCAAT GAAAGAGGTC AATTTACATA ACCCAGCATT TGGAAAGGAT 
AAGAAAGATC GAGACAGCAG CGTAAGGAAT ATTGTTTCTG AATTAACAAT AATTAAAGAG 
CAGCTTTATC ATCCCAACAT TGTACGTTAT TACAAAACAT TTCTGGAAAA TGATAGGTTG 
TACATAGTTA TGGAGCTGAT AGAAGGAGCC CCGCTTGGAG AGCATTTCAG TTCTTTGAAG 
GAAAAACATC ACCATTTTAC TGAAGAAAGA CTATGGAAAA TATTTATACA GCTGTGCTTA 
GCTCTTCGAT ACTTACACAA GGAGAAGAGG ATTGTCCATA GAGATCTGAC ACCAAACAAC 
ATTATGTTGG GGGATAAGGA CAAAGTAACC GTTACCTTTG AAACCATCCA AGGAAACAGA 
CCCCGATTCA GTGGTACATG GGGTGAGACA CAGTCAACAT TTGCCCTAAA TACTGTCACT 
TGCCAAATAA GGTCCATATT GTGCCATCAC ATTACAAAAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NEK10 ( XM_011533413.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011531715.1 Homo sapiens NIMA related kinase 10 (NEK10), transcript variant X20, mRNA.

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CloneID	OHu36997
Clone ID Related Accession (Same CDS sequence)	XM_017005774.2 , XM_017005774.1 , XM_011533413.2
Accession Version	XM_011533413.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2142bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	serine/threonine-protein kinase Nek10 isoform X11
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000003.12) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011533413.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCTGATC AAGATAAAAA GGTGAAGACC ACAGAAAAAT CAACTGATAA ACAGCAAGAA 
ATCACCATCA GGGACTATTC AGATCTTAAA AGACTTCGGT GCCTTTTGAA CGTCCAATCA 
AGCAAACAAC AGCTTCCAGC CATTAACTTC GATAGTGCCC AAAATAGCAT GACGAAGTCT 
GAGCCCGCCA TCAGGGCGGG TGGACACAGA GCTCGGGGTC AGTGGCATGA ATCCACAGAA 
GCTGTTGAAC TTGAAAATTT TAGTATAAAC TACAAGAATG AGAGAAATTT CAGCAAACAT 
CCTCAGCGTA AACTATTTCA GGAGATCTTT ACCGCCTTGG TGAAAAATAG ACTCATAAGC 
AGAGAGTGGG TTAATCGAGC CCCATCTATT CATTTTCTGA GAGTGTTAAT CTGTCTGAGG 
CTACTAATGA GGGATCCATG TTATCAGGAA ATACTCCATA GCTTGGGTGG GATTGAAAAC 
CTAGCTCAGT ATATGGAGAT TGTAGCCAAT GAGTACCTCG GCTATGGAGA AGAGCAGCAC 
ACTGTGGACA AGCTGGTCAA CATGACATAT ATTTTTCAAA AACTTGCTGC AGTCAAAGAT 
CAAAGAGAAT GGGTCACCAC AAGTGGAGCC CACAAGACAT TAGTAAATTT ACTTGGTGCC 
CGAGATACTA ATGTTCTATT GGGTTCCCTT CTGGCTCTGG CTAGTTTAGC AGAAAGTCAA 
GAATGTAGGG AGAAGATAAG TGAACTCAAC ATTGTAGAAA ATCTGTTGAT GATTTTACAT 
GAATATGACT TGCTTTCTAA AAGACTAACA GCGGAGTTGC TGCGCCTACT TTGTGCAGAG 
CCCCAGGTGA AAGAGCAGGT GAAGCTCTAT GAGGGGATAC CGGTCCTCCT CAGTCTGCTC 
CACTCTGACC ACTTGAAGCT CCTCTGGAGC ATTGTCTGGA TTCTGGTACA GGTTTGTGAG 
GACCCTGAGA CCAGCGTGGA AATTCGCATT TGGGGAGGCA TCAAACAGCT TCTTCATATT 
TTACAAGGAG ACAGAAATTT TGTTTCTGAT CACTCCTCCA TTGGAAGCCT GTCCAGTGCA 
AATGCTGCAG GCCGAATCCA GCAGCTTCAT TTATCAGAAG ACTTGAGCCC TAGGGAAATA 
CAAGAAAATA CTTTCTCACT TCAAGCAGCC TGCTGTGCTG CCCTCACTGA GCTGGTGCTC 
AATGACACCA ATGCCCACCA GGTGGTTCAG GAAAATGGTG TATATACAAT AGCAAAATTA 
ATTTTACCAA ATAAGCAAAA GAATGCAGCA AAAAGTAATC TATTACAGTG TTATGCTTTC 
AGAGCCTTGA GATTTCTCTT CAGTATGGAA AGAAACAGAC CACTCTTTAA AAGACTTTTC 
CCCACAGACT TGTTTGAGAT CTTCATTGAC ATAGGGCATT ATGTACGTGA TATCAGTGCT 
TATGAAGAAT TGGTATCCAA GCTGAATTTA TTAGTGGAGG ATGAACTGAA GCAAATTGCT 
GAAAATATTG AAAGCATTAA TCAGAACAAA GCTCCTTTGA AATATATAGG CAACTATGCA 
ATTTTGGATC ATCTTGGAAG TGGAGCTTTT GGCTGTGTTT ACAAGGTTAG AAAGCATAGT 
GGTCAAAATC TTTTAGCAAT GAAAGAGGTC AATTTACATA ACCCAGCATT TGGAAAGGAT 
AAGAAAGATC GAGACAGCAG CGTAAGGAAT ATTGTTTCTG AATTAACAAT AATTAAAGAG 
CAGCTTTATC ATCCCAACAT TGTACGTTAT TACAAAACAT TTCTGGAAAA TGATAGGTTG 
TACATAGTTA TGGAGCTGAT AGAAGGAGCC CCGCTTGGAG AGCATTTCAG TTCTTTGAAG 
GAAAAACATC ACCATTTTAC TGAAGAAAGA CTATGGAAAA TATTTATACA GCTGTGCTTA 
GCTCTTCGAT ACTTACACAA GGAGAAGAGG ATTGTCCATA GAGATCTGAC ACCAAACAAC 
ATTATGTTGG GGGATAAGGA CAAAGTAACC GTTACCTTTG AAACCATCCA AGGAAACAGA 
CCCCGATTCA GTGGTACATG GGGTGAGACA CAGTCAACAT TTGCCCTAAA TACTGTCACT 
TGCCAAATAA GGTCCATATT GTGCCATCAC ATTACAAAAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011531715.1
CDS	275..2416
Translation	MPDQDKKVKTTEKSTDKQQEITIRDYSDLKRLRCLLNVQSSKQQLPAINFDSAQNSMTKSEPAIRAGGHRARGQWHESTEAVELENFSINYKNERNFSKHPQRKLFQEIFTALVKNRLISREWVNRAPSIHFLRVLICLRLLMRDPCYQEILHSLGGIENLAQYMEIVANEYLGYGEEQHTVDKLVNMTYIFQKLAAVKDQREWVTTSGAHKTLVNLLGARDTNVLLGSLLALASLAESQECREKISELNIVENLLMILHEYDLLSKRLTAELLRLLCAEPQVKEQVKLYEGIPVLLSLLHSDHLKLLWSIVWILVQVCEDPETSVEIRIWGGIKQLLHILQGDRNFVSDHSSIGSLSSANAAGRIQQLHLSEDLSPREIQENTFSLQAACCAALTELVLNDTNAHQVVQENGVYTIAKLILPNKQKNAAKSNLLQCYAFRALRFLFSMERNRPLFKRLFPTDLFEIFIDIGHYVRDISAYEELVSKLNLLVEDELKQIAENIESINQNKAPLKYIGNYAILDHLGSGAFGCVYKVRKHSGQNLLAMKEVNLHNPAFGKDKKDRDSSVRNIVSELTIIKEQLYHPNIVRYYKTFLENDRLYIVMELIEGAPLGEHFSSLKEKHHHFTEERLWKIFIQLCLALRYLHKEKRIVHRDLTPNNIMLGDKDKVTVTFETIQGNRPRFSGTWGETQSTFALNTVTCQIRSILCHHITK

Target ORF information:

RefSeq Version	XM_011533413.2
Organism	Homo sapiens(human)
Definition	Homo sapiens NIMA related kinase 10 (NEK10), transcript variant X20, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011533413.2

ORF Insert Sequence:

ATGCCTGATC AAGATAAAAA GGTGAAGACC ACAGAAAAAT CAACTGATAA ACAGCAAGAA 
ATCACCATCA GGGACTATTC AGATCTTAAA AGACTTCGGT GCCTTTTGAA CGTCCAATCA 
AGCAAACAAC AGCTTCCAGC CATTAACTTC GATAGTGCCC AAAATAGCAT GACGAAGTCT 
GAGCCCGCCA TCAGGGCGGG TGGACACAGA GCTCGGGGTC AGTGGCATGA ATCCACAGAA 
GCTGTTGAAC TTGAAAATTT TAGTATAAAC TACAAGAATG AGAGAAATTT CAGCAAACAT 
CCTCAGCGTA AACTATTTCA GGAGATCTTT ACCGCCTTGG TGAAAAATAG ACTCATAAGC 
AGAGAGTGGG TTAATCGAGC CCCATCTATT CATTTTCTGA GAGTGTTAAT CTGTCTGAGG 
CTACTAATGA GGGATCCATG TTATCAGGAA ATACTCCATA GCTTGGGTGG GATTGAAAAC 
CTAGCTCAGT ATATGGAGAT TGTAGCCAAT GAGTACCTCG GCTATGGAGA AGAGCAGCAC 
ACTGTGGACA AGCTGGTCAA CATGACATAT ATTTTTCAAA AACTTGCTGC AGTCAAAGAT 
CAAAGAGAAT GGGTCACCAC AAGTGGAGCC CACAAGACAT TAGTAAATTT ACTTGGTGCC 
CGAGATACTA ATGTTCTATT GGGTTCCCTT CTGGCTCTGG CTAGTTTAGC AGAAAGTCAA 
GAATGTAGGG AGAAGATAAG TGAACTCAAC ATTGTAGAAA ATCTGTTGAT GATTTTACAT 
GAATATGACT TGCTTTCTAA AAGACTAACA GCGGAGTTGC TGCGCCTACT TTGTGCAGAG 
CCCCAGGTGA AAGAGCAGGT GAAGCTCTAT GAGGGGATAC CGGTCCTCCT CAGTCTGCTC 
CACTCTGACC ACTTGAAGCT CCTCTGGAGC ATTGTCTGGA TTCTGGTACA GGTTTGTGAG 
GACCCTGAGA CCAGCGTGGA AATTCGCATT TGGGGAGGCA TCAAACAGCT TCTTCATATT 
TTACAAGGAG ACAGAAATTT TGTTTCTGAT CACTCCTCCA TTGGAAGCCT GTCCAGTGCA 
AATGCTGCAG GCCGAATCCA GCAGCTTCAT TTATCAGAAG ACTTGAGCCC TAGGGAAATA 
CAAGAAAATA CTTTCTCACT TCAAGCAGCC TGCTGTGCTG CCCTCACTGA GCTGGTGCTC 
AATGACACCA ATGCCCACCA GGTGGTTCAG GAAAATGGTG TATATACAAT AGCAAAATTA 
ATTTTACCAA ATAAGCAAAA GAATGCAGCA AAAAGTAATC TATTACAGTG TTATGCTTTC 
AGAGCCTTGA GATTTCTCTT CAGTATGGAA AGAAACAGAC CACTCTTTAA AAGACTTTTC 
CCCACAGACT TGTTTGAGAT CTTCATTGAC ATAGGGCATT ATGTACGTGA TATCAGTGCT 
TATGAAGAAT TGGTATCCAA GCTGAATTTA TTAGTGGAGG ATGAACTGAA GCAAATTGCT 
GAAAATATTG AAAGCATTAA TCAGAACAAA GCTCCTTTGA AATATATAGG CAACTATGCA 
ATTTTGGATC ATCTTGGAAG TGGAGCTTTT GGCTGTGTTT ACAAGGTTAG AAAGCATAGT 
GGTCAAAATC TTTTAGCAAT GAAAGAGGTC AATTTACATA ACCCAGCATT TGGAAAGGAT 
AAGAAAGATC GAGACAGCAG CGTAAGGAAT ATTGTTTCTG AATTAACAAT AATTAAAGAG 
CAGCTTTATC ATCCCAACAT TGTACGTTAT TACAAAACAT TTCTGGAAAA TGATAGGTTG 
TACATAGTTA TGGAGCTGAT AGAAGGAGCC CCGCTTGGAG AGCATTTCAG TTCTTTGAAG 
GAAAAACATC ACCATTTTAC TGAAGAAAGA CTATGGAAAA TATTTATACA GCTGTGCTTA 
GCTCTTCGAT ACTTACACAA GGAGAAGAGG ATTGTCCATA GAGATCTGAC ACCAAACAAC 
ATTATGTTGG GGGATAAGGA CAAAGTAACC GTTACCTTTG AAACCATCCA AGGAAACAGA 
CCCCGATTCA GTGGTACATG GGGTGAGACA CAGTCAACAT TTGCCCTAAA TACTGTCACT 
TGCCAAATAA GGTCCATATT GTGCCATCAC ATTACAAAAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NEK10 ( XM_006713002.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_006713065.1 Homo sapiens NIMA related kinase 10 (NEK10), transcript variant X23, mRNA.

Price & Availability↑

CloneID	OHu36998
Clone ID Related Accession (Same CDS sequence)	XM_006713002.1
Accession Version	XM_006713002.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2130bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	serine/threonine-protein kinase Nek10 isoform X13
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000003.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCTGATC AAGATAAAAA GGTGAAGACC ACAGAAAAAT CAACTGATAA ACAGCAAGAA 
ATCACCATCA GGGACTATTC AGATCTTAAA AGACTTCGGT GCCTTTTGAA CGTCCAATCA 
AGCAAACAAC AGCTTCCAGC CATTAACTTC GATAGTGCCC AAAATAGCAT GACGAAGTCT 
GAGCCCGCCA TCAGGGCGGG TGGACACAGA GCTCGGGGTC AGTGGCATGA ATCCACAGAA 
GCTGTTGAAC TTGAAAATTT TAGTATAAAC TACAAGAATG AGAGAAATTT CAGCAAACAT 
CCTCAGCGTA AACTATTTCA GGAGATCTTT ACCGCCTTGG TGAAAAATAG ACTCATAAGC 
AGAGAGTGGG TTAATCGAGC CCCATCTATT CATTTTCTGA GAGTGTTAAT CTGTCTGAGG 
CTACTAATGA GGGATCCATG TTATCAGGAA ATACTCCATA GCTTGGGTGG GATTGAAAAC 
CTAGCTCAGT ATATGGAGAT TGTAGCCAAT GAGTACCTCG GCTATGGAGA AGAGCAGCAC 
ACTGTGGACA AGCTGGTCAA CATGACATAT ATTTTTCAAA AACTTGCTGC AGTCAAAGAT 
CAAAGAGAAT GGGTCACCAC AAGTGGAGCC CACAAGACAT TAGTAAATTT ACTTGGTGCC 
CGAGATACTA ATGTTCTATT GGGTTCCCTT CTGGCTCTGG CTAGTTTAGC AGAAAGTCAA 
GAATGTAGGG AGAAGATAAG TGAACTCAAC ATTGTAGAAA ATCTGTTGAT GATTTTACAT 
GAATATGACT TGCTTTCTAA AAGACTAACA GCGGAGTTGC TGCGCCTACT TTGTGCAGAG 
CCCCAGGTGA AAGAGCAGGT GAAGCTCTAT GAGGGGATAC CGGTCCTCCT CAGTCTGCTC 
CACTCTGACC ACTTGAAGCT CCTCTGGAGC ATTGTCTGGA TTCTGGTACA GGTTTGTGAG 
GACCCTGAGA CCAGCGTGGA AATTCGCATT TGGGGAGGCA TCAAACAGCT TCTTCATATT 
TTACAAGGAG ACAGAAATTT TGTTTCTGAT CACTCCTCCA TTGGAAGCCT GTCCAGTGCA 
AATGCTGCAG GCCGAATCCA GCAGCTTCAT TTATCAGAAG ACTTGAGCCC TAGGGAAATA 
CAAGAAAATA CTTTCTCACT TCAAGCAGCC TGCTGTGCTG CCCTCACTGA GCTGGTGCTC 
AATGACACCA ATGCCCACCA GGTGGTTCAG GAAAATGGTG TATATACAAT AGCAAAATTA 
ATTTTACCAA ATAAGCAAAA GAATGCAGCA AAAAGTAATC TATTACAGTG TTATGCTTTC 
AGAGCCTTGA GATTTCTCTT CAGTATGGAA AGAAACAGAC CACTCTTTAA AAGACTTTTC 
CCCACAGACT TGTTTGAGAT CTTCATTGAC ATAGGGCATT ATGTACGTGA TATCAGTGCT 
TATGAAGAAT TGGTATCCAA GCTGAATTTA TTAGTGGAGG ATGAACTGAA GCAAATTGCT 
GAAAATATTG AAAGCATTAA TCAGAACAAA GCTCCTTTGA AATATATAGG CAACTATGCA 
ATTTTGGATC ATCTTGGAAG TGGAGCTTTT GGCTGTGTTT ACAAGGTTAG AAAGCATAGT 
GGTCAAAATC TTTTAGCAAT GAAAGAGGTC AATTTACATA ACCCAGCATT TGGAAAGGAT 
AAGAAAGATC GAGACAGCAG CGTAAGGAAT ATTGTTTCTG AATTAACAAT AATTAAAGAG 
CAGCTTTATC ATCCCAACAT TGTACGTTAT TACAAAACAT TTCTGGAAAA TGATAGGTTG 
TACATAGTTA TGGAGCTGAT AGAAGGAGCC CCGCTTGGAG AGCATTTCAG TTCTTTGAAG 
GAAAAACATC ACCATTTTAC TGAAGAAAGA CTATGGAAAA TATTTATACA GCTGTGCTTA 
GCTCTTCGAT ACTTACACAA GGAGAAGAGG ATTGTCCATA GAGATCTGAC ACCAAACAAC 
ATTATGTTGG GGGATAAGGA CAAAGTAACC GTTACTGACT TTGGCCTGGC AAAGCAAAAA 
CAAGAAAACA GTAAACTCAC CTCTGTGGTT GGAACAATCC TGTATTCTTG TGGTTTCATG 
AGAAGATGCA AAGAAACTGT GGCAGGCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006713065.1
CDS	275..2404
Translation	MPDQDKKVKTTEKSTDKQQEITIRDYSDLKRLRCLLNVQSSKQQLPAINFDSAQNSMTKSEPAIRAGGHRARGQWHESTEAVELENFSINYKNERNFSKHPQRKLFQEIFTALVKNRLISREWVNRAPSIHFLRVLICLRLLMRDPCYQEILHSLGGIENLAQYMEIVANEYLGYGEEQHTVDKLVNMTYIFQKLAAVKDQREWVTTSGAHKTLVNLLGARDTNVLLGSLLALASLAESQECREKISELNIVENLLMILHEYDLLSKRLTAELLRLLCAEPQVKEQVKLYEGIPVLLSLLHSDHLKLLWSIVWILVQVCEDPETSVEIRIWGGIKQLLHILQGDRNFVSDHSSIGSLSSANAAGRIQQLHLSEDLSPREIQENTFSLQAACCAALTELVLNDTNAHQVVQENGVYTIAKLILPNKQKNAAKSNLLQCYAFRALRFLFSMERNRPLFKRLFPTDLFEIFIDIGHYVRDISAYEELVSKLNLLVEDELKQIAENIESINQNKAPLKYIGNYAILDHLGSGAFGCVYKVRKHSGQNLLAMKEVNLHNPAFGKDKKDRDSSVRNIVSELTIIKEQLYHPNIVRYYKTFLENDRLYIVMELIEGAPLGEHFSSLKEKHHHFTEERLWKIFIQLCLALRYLHKEKRIVHRDLTPNNIMLGDKDKVTVTDFGLAKQKQENSKLTSVVGTILYSCGFMRRCKETVAG

Target ORF information:

RefSeq Version	XM_006713002.1
Organism	Homo sapiens(human)
Definition	Homo sapiens NIMA related kinase 10 (NEK10), transcript variant X23, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006713002.1

ORF Insert Sequence:

ATGCCTGATC AAGATAAAAA GGTGAAGACC ACAGAAAAAT CAACTGATAA ACAGCAAGAA 
ATCACCATCA GGGACTATTC AGATCTTAAA AGACTTCGGT GCCTTTTGAA CGTCCAATCA 
AGCAAACAAC AGCTTCCAGC CATTAACTTC GATAGTGCCC AAAATAGCAT GACGAAGTCT 
GAGCCCGCCA TCAGGGCGGG TGGACACAGA GCTCGGGGTC AGTGGCATGA ATCCACAGAA 
GCTGTTGAAC TTGAAAATTT TAGTATAAAC TACAAGAATG AGAGAAATTT CAGCAAACAT 
CCTCAGCGTA AACTATTTCA GGAGATCTTT ACCGCCTTGG TGAAAAATAG ACTCATAAGC 
AGAGAGTGGG TTAATCGAGC CCCATCTATT CATTTTCTGA GAGTGTTAAT CTGTCTGAGG 
CTACTAATGA GGGATCCATG TTATCAGGAA ATACTCCATA GCTTGGGTGG GATTGAAAAC 
CTAGCTCAGT ATATGGAGAT TGTAGCCAAT GAGTACCTCG GCTATGGAGA AGAGCAGCAC 
ACTGTGGACA AGCTGGTCAA CATGACATAT ATTTTTCAAA AACTTGCTGC AGTCAAAGAT 
CAAAGAGAAT GGGTCACCAC AAGTGGAGCC CACAAGACAT TAGTAAATTT ACTTGGTGCC 
CGAGATACTA ATGTTCTATT GGGTTCCCTT CTGGCTCTGG CTAGTTTAGC AGAAAGTCAA 
GAATGTAGGG AGAAGATAAG TGAACTCAAC ATTGTAGAAA ATCTGTTGAT GATTTTACAT 
GAATATGACT TGCTTTCTAA AAGACTAACA GCGGAGTTGC TGCGCCTACT TTGTGCAGAG 
CCCCAGGTGA AAGAGCAGGT GAAGCTCTAT GAGGGGATAC CGGTCCTCCT CAGTCTGCTC 
CACTCTGACC ACTTGAAGCT CCTCTGGAGC ATTGTCTGGA TTCTGGTACA GGTTTGTGAG 
GACCCTGAGA CCAGCGTGGA AATTCGCATT TGGGGAGGCA TCAAACAGCT TCTTCATATT 
TTACAAGGAG ACAGAAATTT TGTTTCTGAT CACTCCTCCA TTGGAAGCCT GTCCAGTGCA 
AATGCTGCAG GCCGAATCCA GCAGCTTCAT TTATCAGAAG ACTTGAGCCC TAGGGAAATA 
CAAGAAAATA CTTTCTCACT TCAAGCAGCC TGCTGTGCTG CCCTCACTGA GCTGGTGCTC 
AATGACACCA ATGCCCACCA GGTGGTTCAG GAAAATGGTG TATATACAAT AGCAAAATTA 
ATTTTACCAA ATAAGCAAAA GAATGCAGCA AAAAGTAATC TATTACAGTG TTATGCTTTC 
AGAGCCTTGA GATTTCTCTT CAGTATGGAA AGAAACAGAC CACTCTTTAA AAGACTTTTC 
CCCACAGACT TGTTTGAGAT CTTCATTGAC ATAGGGCATT ATGTACGTGA TATCAGTGCT 
TATGAAGAAT TGGTATCCAA GCTGAATTTA TTAGTGGAGG ATGAACTGAA GCAAATTGCT 
GAAAATATTG AAAGCATTAA TCAGAACAAA GCTCCTTTGA AATATATAGG CAACTATGCA 
ATTTTGGATC ATCTTGGAAG TGGAGCTTTT GGCTGTGTTT ACAAGGTTAG AAAGCATAGT 
GGTCAAAATC TTTTAGCAAT GAAAGAGGTC AATTTACATA ACCCAGCATT TGGAAAGGAT 
AAGAAAGATC GAGACAGCAG CGTAAGGAAT ATTGTTTCTG AATTAACAAT AATTAAAGAG 
CAGCTTTATC ATCCCAACAT TGTACGTTAT TACAAAACAT TTCTGGAAAA TGATAGGTTG 
TACATAGTTA TGGAGCTGAT AGAAGGAGCC CCGCTTGGAG AGCATTTCAG TTCTTTGAAG 
GAAAAACATC ACCATTTTAC TGAAGAAAGA CTATGGAAAA TATTTATACA GCTGTGCTTA 
GCTCTTCGAT ACTTACACAA GGAGAAGAGG ATTGTCCATA GAGATCTGAC ACCAAACAAC 
ATTATGTTGG GGGATAAGGA CAAAGTAACC GTTACTGACT TTGGCCTGGC AAAGCAAAAA 
CAAGAAAACA GTAAACTCAC CTCTGTGGTT GGAACAATCC TGTATTCTTG TGGTTTCATG 
AGAAGATGCA AAGAAACTGT GGCAGGCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NEK10 ( XM_011533414.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011531716.1 Homo sapiens NIMA related kinase 10 (NEK10), transcript variant X24, mRNA.

Price & Availability↑

CloneID	OHu76708
Clone ID Related Accession (Same CDS sequence)	XM_011533414.2
Accession Version	XM_011533414.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2028bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	serine/threonine-protein kinase Nek10 isoform X14
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000003.12) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011533414.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCTGATC AAGATAAAAA GGTGAAGACC ACAGAAAAAT CAACTGATAA ACAGCAAGAA 
ATCACCATCA GGGACTATTC AGATCTTAAA AGACTTCGGT GCCTTTTGAA CGTCCAATCA 
AGCAAACAAC AGCTTCCAGC CATTAACTTC GATAGTGCCC AAAATAGCAT GACGAAGTCT 
GAGCCCGCCA TCAGGGCGGG TGGACACAGA GCTCGGGGTC AGTGGCATGA ATCCACAGAA 
GCTGTTGAAC TTGAAAATTT TAGTATAAAC TACAAGAATG AGAGAAATTT CAGCAAACAT 
CCTCAGCGTA AACTATTTCA GGAGATCTTT ACCGCCTTGG TGAAAAATAG ACTCATAAGC 
AGAGAGTGGG TTAATCGAGC CCCATCTATT CATTTTCTGA GAGTGTTAAT CTGTCTGAGG 
CTACTAATGA GGGATCCATG TTATCAGGAA ATACTCCATA GCTTGGGTGG GATTGAAAAC 
CTAGCTCAGT ATATGGAGAT TGTAGCCAAT GAGTACCTCG GCTATGGAGA AGAGCAGCAC 
ACTGTGGACA AGCTGGTCAA CATGACATAT ATTTTTCAAA AACTTGCTGC AGTCAAAGAT 
CAAAGAGAAT GGGTCACCAC AAGTGGAGCC CACAAGACAT TAGTAAATTT ACTTGGTGCC 
CGAGATACTA ATGTTCTATT GGGTTCCCTT CTGGCTCTGG CTAGTTTAGC AGAAAGTCAA 
GAATGTAGGG AGAAGATAAG TGAACTCAAC ATTGTAGAAA ATCTGTTGAT GATTTTACAT 
GAATATGACT TGCTTTCTAA AAGACTAACA GCGGAGTTGC TGCGCCTACT TTGTGCAGAG 
CCCCAGGTGA AAGAGCAGGT GAAGCTCTAT GAGGGGATAC CGGTCCTCCT CAGTCTGCTC 
CACTCTGACC ACTTGAAGCT CCTCTGGAGC ATTGTCTGGA TTCTGGTACA GGTTTGTGAG 
GACCCTGAGA CCAGCGTGGA AATTCGCATT TGGGGAGGCA TCAAACAGCT TCTTCATATT 
TTACAAGGAG ACAGAAATTT TGTTTCTGAT CACTCCTCCA TTGGAAGCCT GTCCAGTGCA 
AATGCTGCAG GCCGAATCCA GCAGCTTCAT TTATCAGAAG ACTTGAGCCC TAGGGAAATA 
CAAGAAAATA CTTTCTCACT TCAAGCAGCC TGCTGTGCTG CCCTCACTGA GCTGGTGCTC 
AATGACACCA ATGCCCACCA GGTGGTTCAG GAAAATGGTG TATATACAAT AGCAAAATTA 
ATTTTACCAA ATAAGCAAAA GAATGCAGCA AAAAGTAATC TATTACAGTG TTATGCTTTC 
AGAGCCTTGA GATTTCTCTT CAGTATGGAA AGAAACAGAC CACTCTTTAA AAGACTTTTC 
CCCACAGACT TGTTTGAGAT CTTCATTGAC ATAGGGCATT ATGTACGTGA TATCAGTGCT 
TATGAAGAAT TGGTATCCAA GCTGAATTTA TTAGTGGAGG ATGAACTGAA GCAAATTGCT 
GAAAATATTG AAAGCATTAA TCAGAACAAA GCTCCTTTGA AATATATAGG CAACTATGCA 
ATTTTGGATC ATCTTGGAAG TGGAGCTTTT GGCTGTGTTT ACAAGGTTAG AAAGCATAGT 
GGTCAAAATC TTTTAGCAAT GAAAGAGGTC AATTTACATA ACCCAGCATT TGGAAAGGAT 
AAGAAAGATC GAGACAGCAG CGTAAGGAAT ATTGTTTCTG AATTAACAAT AATTAAAGAG 
CAGCTTTATC ATCCCAACAT TGTACGTTAT TACAAAACAT TTCTGGAAAA TGATAGGTTG 
TACATAGTTA TGGAGCTGAT AGAAGGAGCC CCGCTTGGAG AGCATTTCAG TTCTTTGAAG 
GAAAAACATC ACCATTTTAC TGAAGAAAGA CTATGGAAAA TATTTATACA GCTGTGCTTA 
GCTCTTCGAT ACTTACACAA GGAGAAGAGG ATTGTCCATA GAGATCTGAC ACCAAACAAC 
ATTATGTTGG GGGATAAGGA CAAAGTAACC GTTACCCCGA GGTACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011531716.1
CDS	275..2302
Translation	MPDQDKKVKTTEKSTDKQQEITIRDYSDLKRLRCLLNVQSSKQQLPAINFDSAQNSMTKSEPAIRAGGHRARGQWHESTEAVELENFSINYKNERNFSKHPQRKLFQEIFTALVKNRLISREWVNRAPSIHFLRVLICLRLLMRDPCYQEILHSLGGIENLAQYMEIVANEYLGYGEEQHTVDKLVNMTYIFQKLAAVKDQREWVTTSGAHKTLVNLLGARDTNVLLGSLLALASLAESQECREKISELNIVENLLMILHEYDLLSKRLTAELLRLLCAEPQVKEQVKLYEGIPVLLSLLHSDHLKLLWSIVWILVQVCEDPETSVEIRIWGGIKQLLHILQGDRNFVSDHSSIGSLSSANAAGRIQQLHLSEDLSPREIQENTFSLQAACCAALTELVLNDTNAHQVVQENGVYTIAKLILPNKQKNAAKSNLLQCYAFRALRFLFSMERNRPLFKRLFPTDLFEIFIDIGHYVRDISAYEELVSKLNLLVEDELKQIAENIESINQNKAPLKYIGNYAILDHLGSGAFGCVYKVRKHSGQNLLAMKEVNLHNPAFGKDKKDRDSSVRNIVSELTIIKEQLYHPNIVRYYKTFLENDRLYIVMELIEGAPLGEHFSSLKEKHHHFTEERLWKIFIQLCLALRYLHKEKRIVHRDLTPNNIMLGDKDKVTVTPRY

Target ORF information:

RefSeq Version	XM_011533414.2
Organism	Homo sapiens(human)
Definition	Homo sapiens NIMA related kinase 10 (NEK10), transcript variant X24, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011533414.2

ORF Insert Sequence:

ATGCCTGATC AAGATAAAAA GGTGAAGACC ACAGAAAAAT CAACTGATAA ACAGCAAGAA 
ATCACCATCA GGGACTATTC AGATCTTAAA AGACTTCGGT GCCTTTTGAA CGTCCAATCA 
AGCAAACAAC AGCTTCCAGC CATTAACTTC GATAGTGCCC AAAATAGCAT GACGAAGTCT 
GAGCCCGCCA TCAGGGCGGG TGGACACAGA GCTCGGGGTC AGTGGCATGA ATCCACAGAA 
GCTGTTGAAC TTGAAAATTT TAGTATAAAC TACAAGAATG AGAGAAATTT CAGCAAACAT 
CCTCAGCGTA AACTATTTCA GGAGATCTTT ACCGCCTTGG TGAAAAATAG ACTCATAAGC 
AGAGAGTGGG TTAATCGAGC CCCATCTATT CATTTTCTGA GAGTGTTAAT CTGTCTGAGG 
CTACTAATGA GGGATCCATG TTATCAGGAA ATACTCCATA GCTTGGGTGG GATTGAAAAC 
CTAGCTCAGT ATATGGAGAT TGTAGCCAAT GAGTACCTCG GCTATGGAGA AGAGCAGCAC 
ACTGTGGACA AGCTGGTCAA CATGACATAT ATTTTTCAAA AACTTGCTGC AGTCAAAGAT 
CAAAGAGAAT GGGTCACCAC AAGTGGAGCC CACAAGACAT TAGTAAATTT ACTTGGTGCC 
CGAGATACTA ATGTTCTATT GGGTTCCCTT CTGGCTCTGG CTAGTTTAGC AGAAAGTCAA 
GAATGTAGGG AGAAGATAAG TGAACTCAAC ATTGTAGAAA ATCTGTTGAT GATTTTACAT 
GAATATGACT TGCTTTCTAA AAGACTAACA GCGGAGTTGC TGCGCCTACT TTGTGCAGAG 
CCCCAGGTGA AAGAGCAGGT GAAGCTCTAT GAGGGGATAC CGGTCCTCCT CAGTCTGCTC 
CACTCTGACC ACTTGAAGCT CCTCTGGAGC ATTGTCTGGA TTCTGGTACA GGTTTGTGAG 
GACCCTGAGA CCAGCGTGGA AATTCGCATT TGGGGAGGCA TCAAACAGCT TCTTCATATT 
TTACAAGGAG ACAGAAATTT TGTTTCTGAT CACTCCTCCA TTGGAAGCCT GTCCAGTGCA 
AATGCTGCAG GCCGAATCCA GCAGCTTCAT TTATCAGAAG ACTTGAGCCC TAGGGAAATA 
CAAGAAAATA CTTTCTCACT TCAAGCAGCC TGCTGTGCTG CCCTCACTGA GCTGGTGCTC 
AATGACACCA ATGCCCACCA GGTGGTTCAG GAAAATGGTG TATATACAAT AGCAAAATTA 
ATTTTACCAA ATAAGCAAAA GAATGCAGCA AAAAGTAATC TATTACAGTG TTATGCTTTC 
AGAGCCTTGA GATTTCTCTT CAGTATGGAA AGAAACAGAC CACTCTTTAA AAGACTTTTC 
CCCACAGACT TGTTTGAGAT CTTCATTGAC ATAGGGCATT ATGTACGTGA TATCAGTGCT 
TATGAAGAAT TGGTATCCAA GCTGAATTTA TTAGTGGAGG ATGAACTGAA GCAAATTGCT 
GAAAATATTG AAAGCATTAA TCAGAACAAA GCTCCTTTGA AATATATAGG CAACTATGCA 
ATTTTGGATC ATCTTGGAAG TGGAGCTTTT GGCTGTGTTT ACAAGGTTAG AAAGCATAGT 
GGTCAAAATC TTTTAGCAAT GAAAGAGGTC AATTTACATA ACCCAGCATT TGGAAAGGAT 
AAGAAAGATC GAGACAGCAG CGTAAGGAAT ATTGTTTCTG AATTAACAAT AATTAAAGAG 
CAGCTTTATC ATCCCAACAT TGTACGTTAT TACAAAACAT TTCTGGAAAA TGATAGGTTG 
TACATAGTTA TGGAGCTGAT AGAAGGAGCC CCGCTTGGAG AGCATTTCAG TTCTTTGAAG 
GAAAAACATC ACCATTTTAC TGAAGAAAGA CTATGGAAAA TATTTATACA GCTGTGCTTA 
GCTCTTCGAT ACTTACACAA GGAGAAGAGG ATTGTCCATA GAGATCTGAC ACCAAACAAC 
ATTATGTTGG GGGATAAGGA CAAAGTAACC GTTACCCCGA GGTACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NEK10 ( XM_024453374.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_024309142.1 Homo sapiens NIMA related kinase 10 (NEK10), transcript variant X22, mRNA.

Price & Availability↑

CloneID	OHu105312
Clone ID Related Accession (Same CDS sequence)	XM_024453374.1
Accession Version	XM_024453374.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2139bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	serine/threonine-protein kinase Nek10 isoform X12
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000003.12) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACCCCTT TATTCTTTCA GGAAAATGGT GTATATACAA TAGCAAAATT AATTTTACCA 
AATAAGCAAA AGAATGCAGC AAAAAGTAAT CTATTACAGT GTTATGCTTT CAGAGCCTTG 
AGATTTCTCT TCAGTATGGA AAGAAACAGA CCACTCTTTA AAAGACTTTT CCCCACAGAC 
TTGTTTGAGA TCTTCATTGA CATAGGGCAT TATGTACGTG ATATCAGTGC TTATGAAGAA 
TTGGTATCCA AGCTGAATTT ATTAGTGGAG GATGAACTGA AGCAAATTGC TGAAAATATT 
GAAAGCATTA ATCAGAACAA AGCTCCTTTG AAATATATAG GCAACTATGC AATTTTGGAT 
CATCTTGGAA GTGGAGCTTT TGGCTGTGTT TACAAGGTTA GAAAGCATAG TGGTCAAAAT 
CTTTTAGCAA TGAAAGAGGT CAATTTACAT AACCCAGCAT TTGGAAAGGA TAAGAAAGAT 
CGAGACAGCA GCGTAAGGAA TATTGTTTCT GAATTAACAA TAATTAAAGA GCAGCTTTAT 
CATCCCAACA TTGTACGTTA TTACAAAACA TTTCTGGAAA ATGATAGGTT GTACATAGTT 
ATGGAGCTGA TAGAAGGAGC CCCGCTTGGA GAGCATTTCA GTTCTTTGAA GGAAAAACAT 
CACCATTTTA CTGAAGAAAG ACTATGGAAA ATATTTATAC AGCTGTGCTT AGCTCTTCGA 
TACTTACACA AGGAGAAGAG GATTGTCCAT AGAGATCTGA CACCAAACAA CATTATGTTG 
GGGGATAAGG ACAAAGTAAC CGTTACTGAC TTTGGCCTGG CAAAGCAAAA ACAAGAAAAC 
AGTAAACTCA CCTCTGTGGT TGGAACAATC CTGTATTCTT GCCCCGAGGT ACTGAAGAGT 
GAGCCGTATG GGGAGAAGGC TGATGTCTGG GCAGTAGGCT GCATCCTTTA TCAGATGGCG 
ACTTTGAGTC CCCCCTTCTA CAGCACTAAC ATGCTGTCCT TGGCTACAAA AATAGTGGAG 
GCGGTATATG AACCAGTCCC AGAAGGTATC TACTCTGAAA AAGTAACAGA CACCATCAGC 
AGGTGCCTCA CTCCTGATGC GGAAGCTCGT CCAGATATTG TAGAAGTCAG TTCGATGATA 
TCAGATGTCA TGATGAAATA TTTAGACAAC TTATCTACAT CCCAGTTGTC CTTGGAAAAG 
AAGCTAGAAC GGGAACGAAG ACGCACACAA AGGTATTTTA TGGAAGCCAA CCGGAACACC 
GTCACATGTC ACCATGAGCT GGCTGTTCTA TCTCACGAGA CCTTTGAGAA GGCAAGTTTG 
AGTAGCAGCA GCAGTGGAGC AGCCAGCCTG AAAAGTGAAC TTTCAGAAAG CGCAGACCTG 
CCCCCTGAAG GCTTCCAGGC CTCCTATGGT AAAGACGAAG ACAGGGCCTG TGACGAAATC 
CTGTCAGATG ATAACTTCAA CCTGGAAAAT GCTGAGAAAG ATACATATTC AGAGGTAGAT 
GATGAATTGG ACATTTCGGA TAACTCCAGC AGCTCCAGTT CAAGCCCTCT GAAAGAATCT 
ACATTCAACA TTTTAAAGAG AAGTTTTAGT GCTTCAGGAG GAGAAAGACA ATCCCAAACA 
AGGGACTTCA CTGGAGGAAC AGGATCAAGA CCAAGACCAG CATCAGCAGG AATTGCTGTG 
TCCCAGAGGA AAGTGCGTCA GATCAGTGAT CCTATTCAGC AGATATTAAT TCAGCTGCAC 
AAAATAATCT ATATCACACA GCTTCCTCCA GCTTTGCACC ACAATTTGAA AAGAAGGGTT 
ATAGAGAGAT TCAAGAAATC CCTCTTCAGC CAGCAGAGTA ACCCTTGTAA TTTGAAATCT 
GAAATTAAAA AGTTATCTCA GGGATCTCCA GAACCGATTG AGCCCAACTT TTTCACAGCA 
GATTACCATT TATTACATCG TTCATCCGGT GGAAACAGCC TGTCCCCAAA TGACCCTACA 
GGTTTACCAA CCAGCATTGA ATTGGAGGAA GGAATAACAT ATGAACAGAT GCAGACTGTG 
ATTGAAGAAG TCCTTGAGGA AAGTGGCTAT TACAATTTTA CATCTAACAG GTATCATTCC 
TATCCATGGG GGACCAAGAA TCACCCAACC AAAAGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024309142.1
CDS	2782..4920
Translation	MTPLFFQENGVYTIAKLILPNKQKNAAKSNLLQCYAFRALRFLFSMERNRPLFKRLFPTDLFEIFIDIGHYVRDISAYEELVSKLNLLVEDELKQIAENIESINQNKAPLKYIGNYAILDHLGSGAFGCVYKVRKHSGQNLLAMKEVNLHNPAFGKDKKDRDSSVRNIVSELTIIKEQLYHPNIVRYYKTFLENDRLYIVMELIEGAPLGEHFSSLKEKHHHFTEERLWKIFIQLCLALRYLHKEKRIVHRDLTPNNIMLGDKDKVTVTDFGLAKQKQENSKLTSVVGTILYSCPEVLKSEPYGEKADVWAVGCILYQMATLSPPFYSTNMLSLATKIVEAVYEPVPEGIYSEKVTDTISRCLTPDAEARPDIVEVSSMISDVMMKYLDNLSTSQLSLEKKLERERRRTQRYFMEANRNTVTCHHELAVLSHETFEKASLSSSSSGAASLKSELSESADLPPEGFQASYGKDEDRACDEILSDDNFNLENAEKDTYSEVDDELDISDNSSSSSSSPLKESTFNILKRSFSASGGERQSQTRDFTGGTGSRPRPASAGIAVSQRKVRQISDPIQQILIQLHKIIYITQLPPALHHNLKRRVIERFKKSLFSQQSNPCNLKSEIKKLSQGSPEPIEPNFFTADYHLLHRSSGGNSLSPNDPTGLPTSIELEEGITYEQMQTVIEEVLEESGYYNFTSNRYHSYPWGTKNHPTKR

Target ORF information:

RefSeq Version	XM_024453374.1
Organism	Homo sapiens(human)
Definition	Homo sapiens NIMA related kinase 10 (NEK10), transcript variant X22, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024453374.1

ORF Insert Sequence:

ATGACCCCTT TATTCTTTCA GGAAAATGGT GTATATACAA TAGCAAAATT AATTTTACCA 
AATAAGCAAA AGAATGCAGC AAAAAGTAAT CTATTACAGT GTTATGCTTT CAGAGCCTTG 
AGATTTCTCT TCAGTATGGA AAGAAACAGA CCACTCTTTA AAAGACTTTT CCCCACAGAC 
TTGTTTGAGA TCTTCATTGA CATAGGGCAT TATGTACGTG ATATCAGTGC TTATGAAGAA 
TTGGTATCCA AGCTGAATTT ATTAGTGGAG GATGAACTGA AGCAAATTGC TGAAAATATT 
GAAAGCATTA ATCAGAACAA AGCTCCTTTG AAATATATAG GCAACTATGC AATTTTGGAT 
CATCTTGGAA GTGGAGCTTT TGGCTGTGTT TACAAGGTTA GAAAGCATAG TGGTCAAAAT 
CTTTTAGCAA TGAAAGAGGT CAATTTACAT AACCCAGCAT TTGGAAAGGA TAAGAAAGAT 
CGAGACAGCA GCGTAAGGAA TATTGTTTCT GAATTAACAA TAATTAAAGA GCAGCTTTAT 
CATCCCAACA TTGTACGTTA TTACAAAACA TTTCTGGAAA ATGATAGGTT GTACATAGTT 
ATGGAGCTGA TAGAAGGAGC CCCGCTTGGA GAGCATTTCA GTTCTTTGAA GGAAAAACAT 
CACCATTTTA CTGAAGAAAG ACTATGGAAA ATATTTATAC AGCTGTGCTT AGCTCTTCGA 
TACTTACACA AGGAGAAGAG GATTGTCCAT AGAGATCTGA CACCAAACAA CATTATGTTG 
GGGGATAAGG ACAAAGTAAC CGTTACTGAC TTTGGCCTGG CAAAGCAAAA ACAAGAAAAC 
AGTAAACTCA CCTCTGTGGT TGGAACAATC CTGTATTCTT GCCCCGAGGT ACTGAAGAGT 
GAGCCGTATG GGGAGAAGGC TGATGTCTGG GCAGTAGGCT GCATCCTTTA TCAGATGGCG 
ACTTTGAGTC CCCCCTTCTA CAGCACTAAC ATGCTGTCCT TGGCTACAAA AATAGTGGAG 
GCGGTATATG AACCAGTCCC AGAAGGTATC TACTCTGAAA AAGTAACAGA CACCATCAGC 
AGGTGCCTCA CTCCTGATGC GGAAGCTCGT CCAGATATTG TAGAAGTCAG TTCGATGATA 
TCAGATGTCA TGATGAAATA TTTAGACAAC TTATCTACAT CCCAGTTGTC CTTGGAAAAG 
AAGCTAGAAC GGGAACGAAG ACGCACACAA AGGTATTTTA TGGAAGCCAA CCGGAACACC 
GTCACATGTC ACCATGAGCT GGCTGTTCTA TCTCACGAGA CCTTTGAGAA GGCAAGTTTG 
AGTAGCAGCA GCAGTGGAGC AGCCAGCCTG AAAAGTGAAC TTTCAGAAAG CGCAGACCTG 
CCCCCTGAAG GCTTCCAGGC CTCCTATGGT AAAGACGAAG ACAGGGCCTG TGACGAAATC 
CTGTCAGATG ATAACTTCAA CCTGGAAAAT GCTGAGAAAG ATACATATTC AGAGGTAGAT 
GATGAATTGG ACATTTCGGA TAACTCCAGC AGCTCCAGTT CAAGCCCTCT GAAAGAATCT 
ACATTCAACA TTTTAAAGAG AAGTTTTAGT GCTTCAGGAG GAGAAAGACA ATCCCAAACA 
AGGGACTTCA CTGGAGGAAC AGGATCAAGA CCAAGACCAG CATCAGCAGG AATTGCTGTG 
TCCCAGAGGA AAGTGCGTCA GATCAGTGAT CCTATTCAGC AGATATTAAT TCAGCTGCAC 
AAAATAATCT ATATCACACA GCTTCCTCCA GCTTTGCACC ACAATTTGAA AAGAAGGGTT 
ATAGAGAGAT TCAAGAAATC CCTCTTCAGC CAGCAGAGTA ACCCTTGTAA TTTGAAATCT 
GAAATTAAAA AGTTATCTCA GGGATCTCCA GAACCGATTG AGCCCAACTT TTTCACAGCA 
GATTACCATT TATTACATCG TTCATCCGGT GGAAACAGCC TGTCCCCAAA TGACCCTACA 
GGTTTACCAA CCAGCATTGA ATTGGAGGAA GGAATAACAT ATGAACAGAT GCAGACTGTG 
ATTGAAGAAG TCCTTGAGGA AAGTGGCTAT TACAATTTTA CATCTAACAG GTATCATTCC 
TATCCATGGG GGACCAAGAA TCACCCAACC AAAAGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NEK10 ( XM_017005773.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016861262.1 Homo sapiens NIMA related kinase 10 (NEK10), transcript variant X19, mRNA.

Price & Availability↑

CloneID	OHu93839
Clone ID Related Accession (Same CDS sequence)	XM_017005773.1
Accession Version	XM_017005773.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2838bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	serine/threonine-protein kinase Nek10 isoform X10
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000003.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCTGATC AAGATAAAAA GGTGAAGACC ACAGAAAAAT CAACTGATAA ACAGCAAGAA 
ATCACCATCA GGGACTATTC AGATCTTAAA AGACTTCGGT GCCTTTTGAA CGTCCAATCA 
AGCAAACAAC AGCTTCCAGC CATTAACTTC GATAGTGCCC AAAATAGCAT GACGAAGTCT 
GAGCCCGCCA TCAGGGCGGG TGGACACAGA GCTCGGGGTC AGTGGCATGA ATCCACAGAA 
GCTGTTGAAC TTGAAAATTT TAGTATAAAC TACAAGAATG AGAGAAATTT CAGCAAACAT 
CCTCAGCGTA AACTATTTCA GGAGATCTTT ACCGCCTTGG TGAAAAATAG ACTCATAAGC 
AGAGAGTGGG TTAATCGAGC CCCATCTATT CATTTTCTGA GAGTGTTAAT CTGTCTGAGG 
CTACTAATGA GGGATCCATG TTATCAGGAA ATACTCCATA GCTTGGGTGG GATTGAAAAC 
CTAGCTCAGT ATATGGAGAT TGTAGCCAAT GAGTACCTCG GCTATGGAGA AGAGCAGCAC 
ACTGTGGACA AGCTGGTCAA CATGACATAT ATTTTTCAAA AACTTGCTGC AGTCAAAGAT 
CAAAGAGAAT GGGTCACCAC AAGTGGAGCC CACAAGACAT TAGTAAATTT ACTTGGTGCC 
CGAGATACTA ATGTTCTATT GGGTTCCCTT CTGGCTCTGG CTAGTTTAGC AGAAAGTCAA 
GAATGTAGGG AGAAGATAAG TGAACTCAAC ATTGTAGAAA ATCTGTTGAT GATTTTACAT 
GAATATGACT TGCTTTCTAA AAGACTAACA GCGGAGTTGC TGCGCCTACT TTGTGCAGAG 
CCCCAGGTGA AAGAGCAGGT GAAGCTCTAT GAGGGGATAC CGGTCCTCCT CAGTCTGCTC 
CACTCTGACC ACTTGAAGCT CCTCTGGAGC ATTGTCTGGA TTCTGGTACA GGTTTGTGAG 
GACCCTGAGA CCAGCGTGGA AATTCGCATT TGGGGAGGCA TCAAACAGCT TCTTCATATT 
TTACAAGGAG ACAGAAATTT TGTTTCTGAT CACTCCTCCA TTGGAAGCCT GTCCAGTGCA 
AATGCTGCAG GCCGAATCCA GCAGCTTCAT TTATCAGAAG ACTTGAGCCC TAGGGAAATA 
CAAGAAAATA CTTTCTCACT TCAAGCAGCC TGCTGTGCTG CCCTCACTGA GCTGGTGCTC 
AATGACACCA ATGCCCACCA GGTGGTTCAG GAAAATGGTG TATATACAAT AGCAAAATTA 
ATTTTACCAA ATAAGCAAAA GAATGCAGCA AAAAGTAATC TATTACAGTG TTATGCTTTC 
AGAGCCTTGA GATTTCTCTT CAGTATGGAA AGAAACAGAC CACTCTTTAA AAGACTTTTC 
CCCACAGACT TGTTTGAGAT CTTCATTGAC ATAGGGCATT ATGTACGTGA TATCAGTGCT 
TATGAAGAAT TGGTATCCAA GCTGAATTTA TTAGTGGAGG ATGAACTGAA GCAAATTGCT 
GAAAATATTG AAAGCATTAA TCAGAACAAA GCTCCTTTGA AATATATAGG CAACTATGCA 
ATTTTGGATC ATCTTGGAAG TGGAGCTTTT GGCTGTGTTT ACAAGGTTAG AAAGCATAGT 
GGTCAAAATC TTTTAGCAAT GAAAGAGGTC AATTTACATA ACCCAGCATT TGGAAAGGAT 
AAGAAAGATC GAGACAGCAG CGTAAGGAAT ATTGTTTCTG AATTAACAAT AATTAAAGAG 
CAGCTTTATC ATCCCAACAT TGTACGTTAT TACAAAACAT TTCTGGAAAA TGATAGGTTG 
TACATAGTTA TGGAGCTGAT AGAAGGAGCC CCGCTTGGAG AGCATTTCAG TTCTTTGAAG 
GAAAAACATC ACCATTTTAC TGAAGAAAGA CTATGGAAAA TATTTATACA GCTGTGCTTA 
GCTCTTCGAT ACTTACACAA GGAGAAGAGG ATTGTCCATA GAGATCTGAC ACCAAACAAC 
ATTATGTTGG GGGATAAGGA CAAAGTAACC GTTACTGACT TTGGCCTGGC AAAGCAAAAA 
CAAGAAAACA GTAAACTCAC CTCTGTGGTT GGAACAATCC TGTATTCTTG CCCCGAGGTA 
CTGAAGAGTG AGCCGTATGG GGAGAAGGCT GATGTCTGGG CAGTAGGCTG CATCCTTTAT 
CAGATGGCGA CTTTGAGTCC CCCCTTCTAC AGCACTAACA TGCTGTCCTT GGCTACAAAA 
ATAGTGGAGG CGGTATATGA ACCAGTCCCA GAAGGTATCT ACTCTGAAAA AGTAACAGAC 
ACCATCAGCA GGTGCCTCAC TCCTGATGCG GAAGCTCGTC CAGATATTGT AGAAGTCAGT 
TCGATGATAT CAGATGTCAT GATGAAATAT TTAGACAACT TATCTACATC CCAGTTGTCC 
TTGGAAAAGA AGCTAGAACG GGAACGAAGA CGCACACAAA GGTATTTTAT GGAAGCCAAC 
CGGAACACCG TCACATGTCA CCATGAGCTG GCTGTTCTAT CTCACGAGAC CTTTGAGAAG 
GCAAGTTTGA GTAGCAGCAG CAGTGGAGCA GCCAGCCTGA AAAGTGAACT TTCAGAAAGC 
GCAGACCTGC CCCCTGAAGG CTTCCAGGCC TCCTATGGTA AAGACGAAGA CAGGGCCTGT 
GACGAAATCC TGTCAGATGA TAACTTCAAC CTGGAAAATG CTGAGAAAGA TACATATTCA 
GAGGTAGATG ATGAATTGGA CATTTCGGAT AACTCCAGCA GCTCCAGTTC AAGCCCTCTG 
AAAGAATCTA CATTCAACAT TTTAAAGAGA AGTTTTAGTG CTTCAGGAGG AGAAAGACAA 
TCCCAAACAA GGGTATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016861262.1
CDS	275..3112
Translation	MPDQDKKVKTTEKSTDKQQEITIRDYSDLKRLRCLLNVQSSKQQLPAINFDSAQNSMTKSEPAIRAGGHRARGQWHESTEAVELENFSINYKNERNFSKHPQRKLFQEIFTALVKNRLISREWVNRAPSIHFLRVLICLRLLMRDPCYQEILHSLGGIENLAQYMEIVANEYLGYGEEQHTVDKLVNMTYIFQKLAAVKDQREWVTTSGAHKTLVNLLGARDTNVLLGSLLALASLAESQECREKISELNIVENLLMILHEYDLLSKRLTAELLRLLCAEPQVKEQVKLYEGIPVLLSLLHSDHLKLLWSIVWILVQVCEDPETSVEIRIWGGIKQLLHILQGDRNFVSDHSSIGSLSSANAAGRIQQLHLSEDLSPREIQENTFSLQAACCAALTELVLNDTNAHQVVQENGVYTIAKLILPNKQKNAAKSNLLQCYAFRALRFLFSMERNRPLFKRLFPTDLFEIFIDIGHYVRDISAYEELVSKLNLLVEDELKQIAENIESINQNKAPLKYIGNYAILDHLGSGAFGCVYKVRKHSGQNLLAMKEVNLHNPAFGKDKKDRDSSVRNIVSELTIIKEQLYHPNIVRYYKTFLENDRLYIVMELIEGAPLGEHFSSLKEKHHHFTEERLWKIFIQLCLALRYLHKEKRIVHRDLTPNNIMLGDKDKVTVTDFGLAKQKQENSKLTSVVGTILYSCPEVLKSEPYGEKADVWAVGCILYQMATLSPPFYSTNMLSLATKIVEAVYEPVPEGIYSEKVTDTISRCLTPDAEARPDIVEVSSMISDVMMKYLDNLSTSQLSLEKKLERERRRTQRYFMEANRNTVTCHHELAVLSHETFEKASLSSSSSGAASLKSELSESADLPPEGFQASYGKDEDRACDEILSDDNFNLENAEKDTYSEVDDELDISDNSSSSSSSPLKESTFNILKRSFSASGGERQSQTRV

Target ORF information:

RefSeq Version	XM_017005773.1
Organism	Homo sapiens(human)
Definition	Homo sapiens NIMA related kinase 10 (NEK10), transcript variant X19, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017005773.1

ORF Insert Sequence:

ATGCCTGATC AAGATAAAAA GGTGAAGACC ACAGAAAAAT CAACTGATAA ACAGCAAGAA 
ATCACCATCA GGGACTATTC AGATCTTAAA AGACTTCGGT GCCTTTTGAA CGTCCAATCA 
AGCAAACAAC AGCTTCCAGC CATTAACTTC GATAGTGCCC AAAATAGCAT GACGAAGTCT 
GAGCCCGCCA TCAGGGCGGG TGGACACAGA GCTCGGGGTC AGTGGCATGA ATCCACAGAA 
GCTGTTGAAC TTGAAAATTT TAGTATAAAC TACAAGAATG AGAGAAATTT CAGCAAACAT 
CCTCAGCGTA AACTATTTCA GGAGATCTTT ACCGCCTTGG TGAAAAATAG ACTCATAAGC 
AGAGAGTGGG TTAATCGAGC CCCATCTATT CATTTTCTGA GAGTGTTAAT CTGTCTGAGG 
CTACTAATGA GGGATCCATG TTATCAGGAA ATACTCCATA GCTTGGGTGG GATTGAAAAC 
CTAGCTCAGT ATATGGAGAT TGTAGCCAAT GAGTACCTCG GCTATGGAGA AGAGCAGCAC 
ACTGTGGACA AGCTGGTCAA CATGACATAT ATTTTTCAAA AACTTGCTGC AGTCAAAGAT 
CAAAGAGAAT GGGTCACCAC AAGTGGAGCC CACAAGACAT TAGTAAATTT ACTTGGTGCC 
CGAGATACTA ATGTTCTATT GGGTTCCCTT CTGGCTCTGG CTAGTTTAGC AGAAAGTCAA 
GAATGTAGGG AGAAGATAAG TGAACTCAAC ATTGTAGAAA ATCTGTTGAT GATTTTACAT 
GAATATGACT TGCTTTCTAA AAGACTAACA GCGGAGTTGC TGCGCCTACT TTGTGCAGAG 
CCCCAGGTGA AAGAGCAGGT GAAGCTCTAT GAGGGGATAC CGGTCCTCCT CAGTCTGCTC 
CACTCTGACC ACTTGAAGCT CCTCTGGAGC ATTGTCTGGA TTCTGGTACA GGTTTGTGAG 
GACCCTGAGA CCAGCGTGGA AATTCGCATT TGGGGAGGCA TCAAACAGCT TCTTCATATT 
TTACAAGGAG ACAGAAATTT TGTTTCTGAT CACTCCTCCA TTGGAAGCCT GTCCAGTGCA 
AATGCTGCAG GCCGAATCCA GCAGCTTCAT TTATCAGAAG ACTTGAGCCC TAGGGAAATA 
CAAGAAAATA CTTTCTCACT TCAAGCAGCC TGCTGTGCTG CCCTCACTGA GCTGGTGCTC 
AATGACACCA ATGCCCACCA GGTGGTTCAG GAAAATGGTG TATATACAAT AGCAAAATTA 
ATTTTACCAA ATAAGCAAAA GAATGCAGCA AAAAGTAATC TATTACAGTG TTATGCTTTC 
AGAGCCTTGA GATTTCTCTT CAGTATGGAA AGAAACAGAC CACTCTTTAA AAGACTTTTC 
CCCACAGACT TGTTTGAGAT CTTCATTGAC ATAGGGCATT ATGTACGTGA TATCAGTGCT 
TATGAAGAAT TGGTATCCAA GCTGAATTTA TTAGTGGAGG ATGAACTGAA GCAAATTGCT 
GAAAATATTG AAAGCATTAA TCAGAACAAA GCTCCTTTGA AATATATAGG CAACTATGCA 
ATTTTGGATC ATCTTGGAAG TGGAGCTTTT GGCTGTGTTT ACAAGGTTAG AAAGCATAGT 
GGTCAAAATC TTTTAGCAAT GAAAGAGGTC AATTTACATA ACCCAGCATT TGGAAAGGAT 
AAGAAAGATC GAGACAGCAG CGTAAGGAAT ATTGTTTCTG AATTAACAAT AATTAAAGAG 
CAGCTTTATC ATCCCAACAT TGTACGTTAT TACAAAACAT TTCTGGAAAA TGATAGGTTG 
TACATAGTTA TGGAGCTGAT AGAAGGAGCC CCGCTTGGAG AGCATTTCAG TTCTTTGAAG 
GAAAAACATC ACCATTTTAC TGAAGAAAGA CTATGGAAAA TATTTATACA GCTGTGCTTA 
GCTCTTCGAT ACTTACACAA GGAGAAGAGG ATTGTCCATA GAGATCTGAC ACCAAACAAC 
ATTATGTTGG GGGATAAGGA CAAAGTAACC GTTACTGACT TTGGCCTGGC AAAGCAAAAA 
CAAGAAAACA GTAAACTCAC CTCTGTGGTT GGAACAATCC TGTATTCTTG CCCCGAGGTA 
CTGAAGAGTG AGCCGTATGG GGAGAAGGCT GATGTCTGGG CAGTAGGCTG CATCCTTTAT 
CAGATGGCGA CTTTGAGTCC CCCCTTCTAC AGCACTAACA TGCTGTCCTT GGCTACAAAA 
ATAGTGGAGG CGGTATATGA ACCAGTCCCA GAAGGTATCT ACTCTGAAAA AGTAACAGAC 
ACCATCAGCA GGTGCCTCAC TCCTGATGCG GAAGCTCGTC CAGATATTGT AGAAGTCAGT 
TCGATGATAT CAGATGTCAT GATGAAATAT TTAGACAACT TATCTACATC CCAGTTGTCC 
TTGGAAAAGA AGCTAGAACG GGAACGAAGA CGCACACAAA GGTATTTTAT GGAAGCCAAC 
CGGAACACCG TCACATGTCA CCATGAGCTG GCTGTTCTAT CTCACGAGAC CTTTGAGAAG 
GCAAGTTTGA GTAGCAGCAG CAGTGGAGCA GCCAGCCTGA AAAGTGAACT TTCAGAAAGC 
GCAGACCTGC CCCCTGAAGG CTTCCAGGCC TCCTATGGTA AAGACGAAGA CAGGGCCTGT 
GACGAAATCC TGTCAGATGA TAACTTCAAC CTGGAAAATG CTGAGAAAGA TACATATTCA 
GAGGTAGATG ATGAATTGGA CATTTCGGAT AACTCCAGCA GCTCCAGTTC AAGCCCTCTG 
AAAGAATCTA CATTCAACAT TTTAAAGAGA AGTTTTAGTG CTTCAGGAGG AGAAAGACAA 
TCCCAAACAA GGGTATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NEK10 ( XM_017005767.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016861256.1 Homo sapiens NIMA related kinase 10 (NEK10), transcript variant X11, mRNA.

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CloneID	OHu93835
Clone ID Related Accession (Same CDS sequence)	XM_017005767.1 , XM_024453373.1 , XM_017005767.2
Accession Version	XM_017005767.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3432bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	serine/threonine-protein kinase Nek10 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000003.12) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCTGATC AAGATAAAAA GGTGAAGACC ACAGAAAAAT CAACTGATAA ACAGCAAGAA 
ATCACCATCA GGGACTATTC AGATCTTAAA AGACTTCGGT GCCTTTTGAA CGTCCAATCA 
AGCAAACAAC AGCTTCCAGC CATTAACTTC GATAGTGCCC AAAATAGCAT GACGAAGTCT 
GAGCCCGCCA TCAGGGCGGG TGGACACAGA GCTCGGGGTC AGTGGCATGA ATCCACAGAA 
GCTGTTGAAC TTGAAAATTT TAGTATAAAC TACAAGAATG AGAGAAATTT CAGCAAACAT 
CCTCAGCGTA AACTATTTCA GGAGATCTTT ACCGCCTTGG TGAAAAATAG ACTCATAAGC 
AGAGAGTGGG TTAATCGAGC CCCATCTATT CATTTTCTGA GAGTGTTAAT CTGTCTGAGG 
CTACTAATGA GGGATCCATG TTATCAGGAA ATACTCCATA GCTTGGGTGG GATTGAAAAC 
CTAGCTCAGT ATATGGAGAT TGTAGCCAAT GAGTACCTCG GCTATGGAGA AGAGCAGCAC 
ACTGTGGACA AGCTGGTCAA CATGACATAT ATTTTTCAAA AACTTGCTGC AGTCAAAGAT 
CAAAGAGAAT GGGTCACCAC AAGTGGAGCC CACAAGACAT TAGTAAATTT ACTTGGTGCC 
CGAGATACTA ATGTTCTATT GGGTTCCCTT CTGGCTCTGG CTAGTTTAGC AGAAAGACTA 
ACAGCGGAGT TGCTGCGCCT ACTTTGTGCA GAGCCCCAGG TGAAAGAGCA GGTGAAGCTC 
TATGAGGGGA TACCGGTCCT CCTCAGTCTG CTCCACTCTG ACCACTTGAA GCTCCTCTGG 
AGCATTGTCT GGATTCTGGT ACAGGTTTGT GAGGACCCTG AGACCAGCGT GGAAATTCGC 
ATTTGGGGAG GCATCAAACA GCTTCTTCAT ATTTTACAAG GAGACAGAAA TTTTGTTTCT 
GATCACTCCT CCATTGGAAG CCTGTCCAGT GCAAATGCTG CAGGCCGAAT CCAGCAGCTT 
CATTTATCAG AAGACTTGAG CCCTAGGGAA ATACAAGAAA ATACTTTCTC ACTTCAAGCA 
GCCTGCTGTG CTGCCCTCAC TGAGCTGGTG CTCAATGACA CCAATGCCCA CCAGGTGGTT 
CAGGAAAATG GTGTATATAC AATAGCAAAA TTAATTTTAC CAAATAAGCA AAAGAATGCA 
GCAAAAAGTA ATCTATTACA GTGTTATGCT TTCAGAGCCT TGAGATTTCT CTTCAGTATG 
GAAAGAAACA GACCACTCTT TAAAAGACTT TTCCCCACAG ACTTGTTTGA GATCTTCATT 
GACATAGGGC ATTATGTACG TGATATCAGT GCTTATGAAG AATTGGTATC CAAGCTGAAT 
TTATTAGTGG AGGATGAACT GAAGCAAATT GCTGAAAATA TTGAAAGCAT TAATCAGAAC 
AAAGCTCCTT TGAAATATAT AGGCAACTAT GCAATTTTGG ATCATCTTGG AAGTGGAGCT 
TTTGGCTGTG TTTACAAGGT TAGAAAGCAT AGTGGTCAAA ATCTTTTAGC AATGAAAGAG 
GTCAATTTAC ATAACCCAGC ATTTGGAAAG GATAAGAAAG ATCGAGACAG CAGCGTAAGG 
AATATTGTTT CTGAATTAAC AATAATTAAA GAGCAGCTTT ATCATCCCAA CATTGTACGT 
TATTACAAAA CATTTCTGGA AAATGATAGG TTGTACATAG TTATGGAGCT GATAGAAGGA 
GCCCCGCTTG GAGAGCATTT CAGTTCTTTG AAGGAAAAAC ATCACCATTT TACTGAAGAA 
AGACTATGGA AAATATTTAT ACAGCTGTGC TTAGCTCTTC GATACTTACA CAAGGAGAAG 
AGGATTGTCC ATAGAGATCT GACACCAAAC AACATTATGT TGGGGGATAA GGACAAAGTA 
ACCGTTACTG ACTTTGGCCT GGCAAAGCAA AAACAAGAAA ACAGTAAACT CACCTCTGTG 
GTTGGAACAA TCCTGTATTC TTGCCCCGAG GTACTGAAGA GTGAGCCGTA TGGGGAGAAG 
GCTGATGTCT GGGCAGTAGG CTGCATCCTT TATCAGATGG CGACTTTGAG TCCCCCCTTC 
TACAGCACTA ACATGCTGTC CTTGGCTACA AAAATAGTGG AGGCGGTATA TGAACCAGTC 
CCAGAAGGTA TCTACTCTGA AAAAGTAACA GACACCATCA GCAGGTGCCT CACTCCTGAT 
GCGGAAGCTC GTCCAGATAT TGTAGAAGTC AGTTCGATGA TATCAGATGT CATGATGAAA 
TATTTAGACA ACTTATCTAC ATCCCAGTTG TCCTTGGAAA AGAAGCTAGA ACGGGAACGA 
AGACGCACAC AAAGGTATTT TATGGAAGCC AACCGGAACA CCGTCACATG TCACCATGAG 
CTGGCTGTTC TATCTCACGA GACCTTTGAG AAGGCAAGTT TGAGTAGCAG CAGCAGTGGA 
GCAGCCAGCC TGAAAAGTGA ACTTTCAGAA AGCGCAGACC TGCCCCCTGA AGGCTTCCAG 
GCCTCCTATG GTAAAGACGA AGACAGGGCC TGTGACGAAA TCCTGTCAGA TGATAACTTC 
AACCTGGAAA ATGCTGAGAA AGATACATAT TCAGAGGTAG ATGATGAATT GGACATTTCG 
GATAACTCCA GCAGCTCCAG TTCAAGCCCT CTGAAAGAAT CTACATTCAA CATTTTAAAG 
AGAAGTTTTA GTGCTTCAGG AGGAGAAAGA CAATCCCAAA CAAGGGACTT CACTGGAGGA 
ACAGGATCAA GACCAAGACC AGCTTTGCTG CCTCTTGACC TGCTTCTGAA AGTGCCACCC 
CACATGCTCA GGGCCCACAT TAAGGAAATA GAGGCTGAGT TAGTGACAGG GTGGCAGTCC 
CATAGCCTTC CTGCTGTGAT TCTTCGAAAT CTCAAAGATC ATGGGCCACA GATGGGCACA 
TTCTTGTGGC AAGCATCAGC AGGAATTGCT GTGTCCCAGA GGAAAGTGCG TCAGATCAGT 
GATCCTATTC AGCAGATATT AATTCAGCTG CACAAAATAA TCTATATCAC ACAGCTTCCT 
CCAGCTTTGC ACCACAATTT GAAAAGAAGG GTTATAGAGA GATTCAAGAA ATCCCTCTTC 
AGCCAGCAGA GTAACCCTTG TAATTTGAAA TCTGAAATTA AAAAGTTATC TCAGGGATCT 
CCAGAACCGA TTGAGCCCAA CTTTTTCACA GCAGATTACC ATTTATTACA TCGTTCATCC 
GGTGGAAACA GCCTGTCCCC AAATGACCCT ACAGGTTTAC CAACCAGCAT TGAATTGGAG 
GAAGGAATAA CATATGAACA GATGCAGACT GTGATTGAAG AAGTCCTTGA GGAAAGTGGC 
TATTACAATT TTACATCTAA CAGGTATCAT TCCTATCCAT GGGGGACCAA GAATCACCCA 
ACCAAAAGAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016861256.1
CDS	227..3658
Translation	MPDQDKKVKTTEKSTDKQQEITIRDYSDLKRLRCLLNVQSSKQQLPAINFDSAQNSMTKSEPAIRAGGHRARGQWHESTEAVELENFSINYKNERNFSKHPQRKLFQEIFTALVKNRLISREWVNRAPSIHFLRVLICLRLLMRDPCYQEILHSLGGIENLAQYMEIVANEYLGYGEEQHTVDKLVNMTYIFQKLAAVKDQREWVTTSGAHKTLVNLLGARDTNVLLGSLLALASLAERLTAELLRLLCAEPQVKEQVKLYEGIPVLLSLLHSDHLKLLWSIVWILVQVCEDPETSVEIRIWGGIKQLLHILQGDRNFVSDHSSIGSLSSANAAGRIQQLHLSEDLSPREIQENTFSLQAACCAALTELVLNDTNAHQVVQENGVYTIAKLILPNKQKNAAKSNLLQCYAFRALRFLFSMERNRPLFKRLFPTDLFEIFIDIGHYVRDISAYEELVSKLNLLVEDELKQIAENIESINQNKAPLKYIGNYAILDHLGSGAFGCVYKVRKHSGQNLLAMKEVNLHNPAFGKDKKDRDSSVRNIVSELTIIKEQLYHPNIVRYYKTFLENDRLYIVMELIEGAPLGEHFSSLKEKHHHFTEERLWKIFIQLCLALRYLHKEKRIVHRDLTPNNIMLGDKDKVTVTDFGLAKQKQENSKLTSVVGTILYSCPEVLKSEPYGEKADVWAVGCILYQMATLSPPFYSTNMLSLATKIVEAVYEPVPEGIYSEKVTDTISRCLTPDAEARPDIVEVSSMISDVMMKYLDNLSTSQLSLEKKLERERRRTQRYFMEANRNTVTCHHELAVLSHETFEKASLSSSSSGAASLKSELSESADLPPEGFQASYGKDEDRACDEILSDDNFNLENAEKDTYSEVDDELDISDNSSSSSSSPLKESTFNILKRSFSASGGERQSQTRDFTGGTGSRPRPALLPLDLLLKVPPHMLRAHIKEIEAELVTGWQSHSLPAVILRNLKDHGPQMGTFLWQASAGIAVSQRKVRQISDPIQQILIQLHKIIYITQLPPALHHNLKRRVIERFKKSLFSQQSNPCNLKSEIKKLSQGSPEPIEPNFFTADYHLLHRSSGGNSLSPNDPTGLPTSIELEEGITYEQMQTVIEEVLEESGYYNFTSNRYHSYPWGTKNHPTKR

Target ORF information:

RefSeq Version	XM_017005767.1
Organism	Homo sapiens(human)
Definition	Homo sapiens NIMA related kinase 10 (NEK10), transcript variant X11, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017005767.1

ORF Insert Sequence:

ATGCCTGATC AAGATAAAAA GGTGAAGACC ACAGAAAAAT CAACTGATAA ACAGCAAGAA 
ATCACCATCA GGGACTATTC AGATCTTAAA AGACTTCGGT GCCTTTTGAA CGTCCAATCA 
AGCAAACAAC AGCTTCCAGC CATTAACTTC GATAGTGCCC AAAATAGCAT GACGAAGTCT 
GAGCCCGCCA TCAGGGCGGG TGGACACAGA GCTCGGGGTC AGTGGCATGA ATCCACAGAA 
GCTGTTGAAC TTGAAAATTT TAGTATAAAC TACAAGAATG AGAGAAATTT CAGCAAACAT 
CCTCAGCGTA AACTATTTCA GGAGATCTTT ACCGCCTTGG TGAAAAATAG ACTCATAAGC 
AGAGAGTGGG TTAATCGAGC CCCATCTATT CATTTTCTGA GAGTGTTAAT CTGTCTGAGG 
CTACTAATGA GGGATCCATG TTATCAGGAA ATACTCCATA GCTTGGGTGG GATTGAAAAC 
CTAGCTCAGT ATATGGAGAT TGTAGCCAAT GAGTACCTCG GCTATGGAGA AGAGCAGCAC 
ACTGTGGACA AGCTGGTCAA CATGACATAT ATTTTTCAAA AACTTGCTGC AGTCAAAGAT 
CAAAGAGAAT GGGTCACCAC AAGTGGAGCC CACAAGACAT TAGTAAATTT ACTTGGTGCC 
CGAGATACTA ATGTTCTATT GGGTTCCCTT CTGGCTCTGG CTAGTTTAGC AGAAAGACTA 
ACAGCGGAGT TGCTGCGCCT ACTTTGTGCA GAGCCCCAGG TGAAAGAGCA GGTGAAGCTC 
TATGAGGGGA TACCGGTCCT CCTCAGTCTG CTCCACTCTG ACCACTTGAA GCTCCTCTGG 
AGCATTGTCT GGATTCTGGT ACAGGTTTGT GAGGACCCTG AGACCAGCGT GGAAATTCGC 
ATTTGGGGAG GCATCAAACA GCTTCTTCAT ATTTTACAAG GAGACAGAAA TTTTGTTTCT 
GATCACTCCT CCATTGGAAG CCTGTCCAGT GCAAATGCTG CAGGCCGAAT CCAGCAGCTT 
CATTTATCAG AAGACTTGAG CCCTAGGGAA ATACAAGAAA ATACTTTCTC ACTTCAAGCA 
GCCTGCTGTG CTGCCCTCAC TGAGCTGGTG CTCAATGACA CCAATGCCCA CCAGGTGGTT 
CAGGAAAATG GTGTATATAC AATAGCAAAA TTAATTTTAC CAAATAAGCA AAAGAATGCA 
GCAAAAAGTA ATCTATTACA GTGTTATGCT TTCAGAGCCT TGAGATTTCT CTTCAGTATG 
GAAAGAAACA GACCACTCTT TAAAAGACTT TTCCCCACAG ACTTGTTTGA GATCTTCATT 
GACATAGGGC ATTATGTACG TGATATCAGT GCTTATGAAG AATTGGTATC CAAGCTGAAT 
TTATTAGTGG AGGATGAACT GAAGCAAATT GCTGAAAATA TTGAAAGCAT TAATCAGAAC 
AAAGCTCCTT TGAAATATAT AGGCAACTAT GCAATTTTGG ATCATCTTGG AAGTGGAGCT 
TTTGGCTGTG TTTACAAGGT TAGAAAGCAT AGTGGTCAAA ATCTTTTAGC AATGAAAGAG 
GTCAATTTAC ATAACCCAGC ATTTGGAAAG GATAAGAAAG ATCGAGACAG CAGCGTAAGG 
AATATTGTTT CTGAATTAAC AATAATTAAA GAGCAGCTTT ATCATCCCAA CATTGTACGT 
TATTACAAAA CATTTCTGGA AAATGATAGG TTGTACATAG TTATGGAGCT GATAGAAGGA 
GCCCCGCTTG GAGAGCATTT CAGTTCTTTG AAGGAAAAAC ATCACCATTT TACTGAAGAA 
AGACTATGGA AAATATTTAT ACAGCTGTGC TTAGCTCTTC GATACTTACA CAAGGAGAAG 
AGGATTGTCC ATAGAGATCT GACACCAAAC AACATTATGT TGGGGGATAA GGACAAAGTA 
ACCGTTACTG ACTTTGGCCT GGCAAAGCAA AAACAAGAAA ACAGTAAACT CACCTCTGTG 
GTTGGAACAA TCCTGTATTC TTGCCCCGAG GTACTGAAGA GTGAGCCGTA TGGGGAGAAG 
GCTGATGTCT GGGCAGTAGG CTGCATCCTT TATCAGATGG CGACTTTGAG TCCCCCCTTC 
TACAGCACTA ACATGCTGTC CTTGGCTACA AAAATAGTGG AGGCGGTATA TGAACCAGTC 
CCAGAAGGTA TCTACTCTGA AAAAGTAACA GACACCATCA GCAGGTGCCT CACTCCTGAT 
GCGGAAGCTC GTCCAGATAT TGTAGAAGTC AGTTCGATGA TATCAGATGT CATGATGAAA 
TATTTAGACA ACTTATCTAC ATCCCAGTTG TCCTTGGAAA AGAAGCTAGA ACGGGAACGA 
AGACGCACAC AAAGGTATTT TATGGAAGCC AACCGGAACA CCGTCACATG TCACCATGAG 
CTGGCTGTTC TATCTCACGA GACCTTTGAG AAGGCAAGTT TGAGTAGCAG CAGCAGTGGA 
GCAGCCAGCC TGAAAAGTGA ACTTTCAGAA AGCGCAGACC TGCCCCCTGA AGGCTTCCAG 
GCCTCCTATG GTAAAGACGA AGACAGGGCC TGTGACGAAA TCCTGTCAGA TGATAACTTC 
AACCTGGAAA ATGCTGAGAA AGATACATAT TCAGAGGTAG ATGATGAATT GGACATTTCG 
GATAACTCCA GCAGCTCCAG TTCAAGCCCT CTGAAAGAAT CTACATTCAA CATTTTAAAG 
AGAAGTTTTA GTGCTTCAGG AGGAGAAAGA CAATCCCAAA CAAGGGACTT CACTGGAGGA 
ACAGGATCAA GACCAAGACC AGCTTTGCTG CCTCTTGACC TGCTTCTGAA AGTGCCACCC 
CACATGCTCA GGGCCCACAT TAAGGAAATA GAGGCTGAGT TAGTGACAGG GTGGCAGTCC 
CATAGCCTTC CTGCTGTGAT TCTTCGAAAT CTCAAAGATC ATGGGCCACA GATGGGCACA 
TTCTTGTGGC AAGCATCAGC AGGAATTGCT GTGTCCCAGA GGAAAGTGCG TCAGATCAGT 
GATCCTATTC AGCAGATATT AATTCAGCTG CACAAAATAA TCTATATCAC ACAGCTTCCT 
CCAGCTTTGC ACCACAATTT GAAAAGAAGG GTTATAGAGA GATTCAAGAA ATCCCTCTTC 
AGCCAGCAGA GTAACCCTTG TAATTTGAAA TCTGAAATTA AAAAGTTATC TCAGGGATCT 
CCAGAACCGA TTGAGCCCAA CTTTTTCACA GCAGATTACC ATTTATTACA TCGTTCATCC 
GGTGGAAACA GCCTGTCCCC AAATGACCCT ACAGGTTTAC CAACCAGCAT TGAATTGGAG 
GAAGGAATAA CATATGAACA GATGCAGACT GTGATTGAAG AAGTCCTTGA GGAAAGTGGC 
TATTACAATT TTACATCTAA CAGGTATCAT TCCTATCCAT GGGGGACCAA GAATCACCCA 
ACCAAAAGAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NEK10 ( XM_024453373.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_024309141.1 Homo sapiens NIMA related kinase 10 (NEK10), transcript variant X11, mRNA.

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CloneID	OHu93835
Clone ID Related Accession (Same CDS sequence)	XM_017005767.1 , XM_024453373.1 , XM_017005767.2
Accession Version	XM_024453373.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3432bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	serine/threonine-protein kinase Nek10 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000003.12) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCTGATC AAGATAAAAA GGTGAAGACC ACAGAAAAAT CAACTGATAA ACAGCAAGAA 
ATCACCATCA GGGACTATTC AGATCTTAAA AGACTTCGGT GCCTTTTGAA CGTCCAATCA 
AGCAAACAAC AGCTTCCAGC CATTAACTTC GATAGTGCCC AAAATAGCAT GACGAAGTCT 
GAGCCCGCCA TCAGGGCGGG TGGACACAGA GCTCGGGGTC AGTGGCATGA ATCCACAGAA 
GCTGTTGAAC TTGAAAATTT TAGTATAAAC TACAAGAATG AGAGAAATTT CAGCAAACAT 
CCTCAGCGTA AACTATTTCA GGAGATCTTT ACCGCCTTGG TGAAAAATAG ACTCATAAGC 
AGAGAGTGGG TTAATCGAGC CCCATCTATT CATTTTCTGA GAGTGTTAAT CTGTCTGAGG 
CTACTAATGA GGGATCCATG TTATCAGGAA ATACTCCATA GCTTGGGTGG GATTGAAAAC 
CTAGCTCAGT ATATGGAGAT TGTAGCCAAT GAGTACCTCG GCTATGGAGA AGAGCAGCAC 
ACTGTGGACA AGCTGGTCAA CATGACATAT ATTTTTCAAA AACTTGCTGC AGTCAAAGAT 
CAAAGAGAAT GGGTCACCAC AAGTGGAGCC CACAAGACAT TAGTAAATTT ACTTGGTGCC 
CGAGATACTA ATGTTCTATT GGGTTCCCTT CTGGCTCTGG CTAGTTTAGC AGAAAGACTA 
ACAGCGGAGT TGCTGCGCCT ACTTTGTGCA GAGCCCCAGG TGAAAGAGCA GGTGAAGCTC 
TATGAGGGGA TACCGGTCCT CCTCAGTCTG CTCCACTCTG ACCACTTGAA GCTCCTCTGG 
AGCATTGTCT GGATTCTGGT ACAGGTTTGT GAGGACCCTG AGACCAGCGT GGAAATTCGC 
ATTTGGGGAG GCATCAAACA GCTTCTTCAT ATTTTACAAG GAGACAGAAA TTTTGTTTCT 
GATCACTCCT CCATTGGAAG CCTGTCCAGT GCAAATGCTG CAGGCCGAAT CCAGCAGCTT 
CATTTATCAG AAGACTTGAG CCCTAGGGAA ATACAAGAAA ATACTTTCTC ACTTCAAGCA 
GCCTGCTGTG CTGCCCTCAC TGAGCTGGTG CTCAATGACA CCAATGCCCA CCAGGTGGTT 
CAGGAAAATG GTGTATATAC AATAGCAAAA TTAATTTTAC CAAATAAGCA AAAGAATGCA 
GCAAAAAGTA ATCTATTACA GTGTTATGCT TTCAGAGCCT TGAGATTTCT CTTCAGTATG 
GAAAGAAACA GACCACTCTT TAAAAGACTT TTCCCCACAG ACTTGTTTGA GATCTTCATT 
GACATAGGGC ATTATGTACG TGATATCAGT GCTTATGAAG AATTGGTATC CAAGCTGAAT 
TTATTAGTGG AGGATGAACT GAAGCAAATT GCTGAAAATA TTGAAAGCAT TAATCAGAAC 
AAAGCTCCTT TGAAATATAT AGGCAACTAT GCAATTTTGG ATCATCTTGG AAGTGGAGCT 
TTTGGCTGTG TTTACAAGGT TAGAAAGCAT AGTGGTCAAA ATCTTTTAGC AATGAAAGAG 
GTCAATTTAC ATAACCCAGC ATTTGGAAAG GATAAGAAAG ATCGAGACAG CAGCGTAAGG 
AATATTGTTT CTGAATTAAC AATAATTAAA GAGCAGCTTT ATCATCCCAA CATTGTACGT 
TATTACAAAA CATTTCTGGA AAATGATAGG TTGTACATAG TTATGGAGCT GATAGAAGGA 
GCCCCGCTTG GAGAGCATTT CAGTTCTTTG AAGGAAAAAC ATCACCATTT TACTGAAGAA 
AGACTATGGA AAATATTTAT ACAGCTGTGC TTAGCTCTTC GATACTTACA CAAGGAGAAG 
AGGATTGTCC ATAGAGATCT GACACCAAAC AACATTATGT TGGGGGATAA GGACAAAGTA 
ACCGTTACTG ACTTTGGCCT GGCAAAGCAA AAACAAGAAA ACAGTAAACT CACCTCTGTG 
GTTGGAACAA TCCTGTATTC TTGCCCCGAG GTACTGAAGA GTGAGCCGTA TGGGGAGAAG 
GCTGATGTCT GGGCAGTAGG CTGCATCCTT TATCAGATGG CGACTTTGAG TCCCCCCTTC 
TACAGCACTA ACATGCTGTC CTTGGCTACA AAAATAGTGG AGGCGGTATA TGAACCAGTC 
CCAGAAGGTA TCTACTCTGA AAAAGTAACA GACACCATCA GCAGGTGCCT CACTCCTGAT 
GCGGAAGCTC GTCCAGATAT TGTAGAAGTC AGTTCGATGA TATCAGATGT CATGATGAAA 
TATTTAGACA ACTTATCTAC ATCCCAGTTG TCCTTGGAAA AGAAGCTAGA ACGGGAACGA 
AGACGCACAC AAAGGTATTT TATGGAAGCC AACCGGAACA CCGTCACATG TCACCATGAG 
CTGGCTGTTC TATCTCACGA GACCTTTGAG AAGGCAAGTT TGAGTAGCAG CAGCAGTGGA 
GCAGCCAGCC TGAAAAGTGA ACTTTCAGAA AGCGCAGACC TGCCCCCTGA AGGCTTCCAG 
GCCTCCTATG GTAAAGACGA AGACAGGGCC TGTGACGAAA TCCTGTCAGA TGATAACTTC 
AACCTGGAAA ATGCTGAGAA AGATACATAT TCAGAGGTAG ATGATGAATT GGACATTTCG 
GATAACTCCA GCAGCTCCAG TTCAAGCCCT CTGAAAGAAT CTACATTCAA CATTTTAAAG 
AGAAGTTTTA GTGCTTCAGG AGGAGAAAGA CAATCCCAAA CAAGGGACTT CACTGGAGGA 
ACAGGATCAA GACCAAGACC AGCTTTGCTG CCTCTTGACC TGCTTCTGAA AGTGCCACCC 
CACATGCTCA GGGCCCACAT TAAGGAAATA GAGGCTGAGT TAGTGACAGG GTGGCAGTCC 
CATAGCCTTC CTGCTGTGAT TCTTCGAAAT CTCAAAGATC ATGGGCCACA GATGGGCACA 
TTCTTGTGGC AAGCATCAGC AGGAATTGCT GTGTCCCAGA GGAAAGTGCG TCAGATCAGT 
GATCCTATTC AGCAGATATT AATTCAGCTG CACAAAATAA TCTATATCAC ACAGCTTCCT 
CCAGCTTTGC ACCACAATTT GAAAAGAAGG GTTATAGAGA GATTCAAGAA ATCCCTCTTC 
AGCCAGCAGA GTAACCCTTG TAATTTGAAA TCTGAAATTA AAAAGTTATC TCAGGGATCT 
CCAGAACCGA TTGAGCCCAA CTTTTTCACA GCAGATTACC ATTTATTACA TCGTTCATCC 
GGTGGAAACA GCCTGTCCCC AAATGACCCT ACAGGTTTAC CAACCAGCAT TGAATTGGAG 
GAAGGAATAA CATATGAACA GATGCAGACT GTGATTGAAG AAGTCCTTGA GGAAAGTGGC 
TATTACAATT TTACATCTAA CAGGTATCAT TCCTATCCAT GGGGGACCAA GAATCACCCA 
ACCAAAAGAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024309141.1
CDS	275..3706
Translation	MPDQDKKVKTTEKSTDKQQEITIRDYSDLKRLRCLLNVQSSKQQLPAINFDSAQNSMTKSEPAIRAGGHRARGQWHESTEAVELENFSINYKNERNFSKHPQRKLFQEIFTALVKNRLISREWVNRAPSIHFLRVLICLRLLMRDPCYQEILHSLGGIENLAQYMEIVANEYLGYGEEQHTVDKLVNMTYIFQKLAAVKDQREWVTTSGAHKTLVNLLGARDTNVLLGSLLALASLAERLTAELLRLLCAEPQVKEQVKLYEGIPVLLSLLHSDHLKLLWSIVWILVQVCEDPETSVEIRIWGGIKQLLHILQGDRNFVSDHSSIGSLSSANAAGRIQQLHLSEDLSPREIQENTFSLQAACCAALTELVLNDTNAHQVVQENGVYTIAKLILPNKQKNAAKSNLLQCYAFRALRFLFSMERNRPLFKRLFPTDLFEIFIDIGHYVRDISAYEELVSKLNLLVEDELKQIAENIESINQNKAPLKYIGNYAILDHLGSGAFGCVYKVRKHSGQNLLAMKEVNLHNPAFGKDKKDRDSSVRNIVSELTIIKEQLYHPNIVRYYKTFLENDRLYIVMELIEGAPLGEHFSSLKEKHHHFTEERLWKIFIQLCLALRYLHKEKRIVHRDLTPNNIMLGDKDKVTVTDFGLAKQKQENSKLTSVVGTILYSCPEVLKSEPYGEKADVWAVGCILYQMATLSPPFYSTNMLSLATKIVEAVYEPVPEGIYSEKVTDTISRCLTPDAEARPDIVEVSSMISDVMMKYLDNLSTSQLSLEKKLERERRRTQRYFMEANRNTVTCHHELAVLSHETFEKASLSSSSSGAASLKSELSESADLPPEGFQASYGKDEDRACDEILSDDNFNLENAEKDTYSEVDDELDISDNSSSSSSSPLKESTFNILKRSFSASGGERQSQTRDFTGGTGSRPRPALLPLDLLLKVPPHMLRAHIKEIEAELVTGWQSHSLPAVILRNLKDHGPQMGTFLWQASAGIAVSQRKVRQISDPIQQILIQLHKIIYITQLPPALHHNLKRRVIERFKKSLFSQQSNPCNLKSEIKKLSQGSPEPIEPNFFTADYHLLHRSSGGNSLSPNDPTGLPTSIELEEGITYEQMQTVIEEVLEESGYYNFTSNRYHSYPWGTKNHPTKR

Target ORF information:

RefSeq Version	XM_024453373.1
Organism	Homo sapiens(human)
Definition	Homo sapiens NIMA related kinase 10 (NEK10), transcript variant X11, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024453373.1

ORF Insert Sequence:

ATGCCTGATC AAGATAAAAA GGTGAAGACC ACAGAAAAAT CAACTGATAA ACAGCAAGAA 
ATCACCATCA GGGACTATTC AGATCTTAAA AGACTTCGGT GCCTTTTGAA CGTCCAATCA 
AGCAAACAAC AGCTTCCAGC CATTAACTTC GATAGTGCCC AAAATAGCAT GACGAAGTCT 
GAGCCCGCCA TCAGGGCGGG TGGACACAGA GCTCGGGGTC AGTGGCATGA ATCCACAGAA 
GCTGTTGAAC TTGAAAATTT TAGTATAAAC TACAAGAATG AGAGAAATTT CAGCAAACAT 
CCTCAGCGTA AACTATTTCA GGAGATCTTT ACCGCCTTGG TGAAAAATAG ACTCATAAGC 
AGAGAGTGGG TTAATCGAGC CCCATCTATT CATTTTCTGA GAGTGTTAAT CTGTCTGAGG 
CTACTAATGA GGGATCCATG TTATCAGGAA ATACTCCATA GCTTGGGTGG GATTGAAAAC 
CTAGCTCAGT ATATGGAGAT TGTAGCCAAT GAGTACCTCG GCTATGGAGA AGAGCAGCAC 
ACTGTGGACA AGCTGGTCAA CATGACATAT ATTTTTCAAA AACTTGCTGC AGTCAAAGAT 
CAAAGAGAAT GGGTCACCAC AAGTGGAGCC CACAAGACAT TAGTAAATTT ACTTGGTGCC 
CGAGATACTA ATGTTCTATT GGGTTCCCTT CTGGCTCTGG CTAGTTTAGC AGAAAGACTA 
ACAGCGGAGT TGCTGCGCCT ACTTTGTGCA GAGCCCCAGG TGAAAGAGCA GGTGAAGCTC 
TATGAGGGGA TACCGGTCCT CCTCAGTCTG CTCCACTCTG ACCACTTGAA GCTCCTCTGG 
AGCATTGTCT GGATTCTGGT ACAGGTTTGT GAGGACCCTG AGACCAGCGT GGAAATTCGC 
ATTTGGGGAG GCATCAAACA GCTTCTTCAT ATTTTACAAG GAGACAGAAA TTTTGTTTCT 
GATCACTCCT CCATTGGAAG CCTGTCCAGT GCAAATGCTG CAGGCCGAAT CCAGCAGCTT 
CATTTATCAG AAGACTTGAG CCCTAGGGAA ATACAAGAAA ATACTTTCTC ACTTCAAGCA 
GCCTGCTGTG CTGCCCTCAC TGAGCTGGTG CTCAATGACA CCAATGCCCA CCAGGTGGTT 
CAGGAAAATG GTGTATATAC AATAGCAAAA TTAATTTTAC CAAATAAGCA AAAGAATGCA 
GCAAAAAGTA ATCTATTACA GTGTTATGCT TTCAGAGCCT TGAGATTTCT CTTCAGTATG 
GAAAGAAACA GACCACTCTT TAAAAGACTT TTCCCCACAG ACTTGTTTGA GATCTTCATT 
GACATAGGGC ATTATGTACG TGATATCAGT GCTTATGAAG AATTGGTATC CAAGCTGAAT 
TTATTAGTGG AGGATGAACT GAAGCAAATT GCTGAAAATA TTGAAAGCAT TAATCAGAAC 
AAAGCTCCTT TGAAATATAT AGGCAACTAT GCAATTTTGG ATCATCTTGG AAGTGGAGCT 
TTTGGCTGTG TTTACAAGGT TAGAAAGCAT AGTGGTCAAA ATCTTTTAGC AATGAAAGAG 
GTCAATTTAC ATAACCCAGC ATTTGGAAAG GATAAGAAAG ATCGAGACAG CAGCGTAAGG 
AATATTGTTT CTGAATTAAC AATAATTAAA GAGCAGCTTT ATCATCCCAA CATTGTACGT 
TATTACAAAA CATTTCTGGA AAATGATAGG TTGTACATAG TTATGGAGCT GATAGAAGGA 
GCCCCGCTTG GAGAGCATTT CAGTTCTTTG AAGGAAAAAC ATCACCATTT TACTGAAGAA 
AGACTATGGA AAATATTTAT ACAGCTGTGC TTAGCTCTTC GATACTTACA CAAGGAGAAG 
AGGATTGTCC ATAGAGATCT GACACCAAAC AACATTATGT TGGGGGATAA GGACAAAGTA 
ACCGTTACTG ACTTTGGCCT GGCAAAGCAA AAACAAGAAA ACAGTAAACT CACCTCTGTG 
GTTGGAACAA TCCTGTATTC TTGCCCCGAG GTACTGAAGA GTGAGCCGTA TGGGGAGAAG 
GCTGATGTCT GGGCAGTAGG CTGCATCCTT TATCAGATGG CGACTTTGAG TCCCCCCTTC 
TACAGCACTA ACATGCTGTC CTTGGCTACA AAAATAGTGG AGGCGGTATA TGAACCAGTC 
CCAGAAGGTA TCTACTCTGA AAAAGTAACA GACACCATCA GCAGGTGCCT CACTCCTGAT 
GCGGAAGCTC GTCCAGATAT TGTAGAAGTC AGTTCGATGA TATCAGATGT CATGATGAAA 
TATTTAGACA ACTTATCTAC ATCCCAGTTG TCCTTGGAAA AGAAGCTAGA ACGGGAACGA 
AGACGCACAC AAAGGTATTT TATGGAAGCC AACCGGAACA CCGTCACATG TCACCATGAG 
CTGGCTGTTC TATCTCACGA GACCTTTGAG AAGGCAAGTT TGAGTAGCAG CAGCAGTGGA 
GCAGCCAGCC TGAAAAGTGA ACTTTCAGAA AGCGCAGACC TGCCCCCTGA AGGCTTCCAG 
GCCTCCTATG GTAAAGACGA AGACAGGGCC TGTGACGAAA TCCTGTCAGA TGATAACTTC 
AACCTGGAAA ATGCTGAGAA AGATACATAT TCAGAGGTAG ATGATGAATT GGACATTTCG 
GATAACTCCA GCAGCTCCAG TTCAAGCCCT CTGAAAGAAT CTACATTCAA CATTTTAAAG 
AGAAGTTTTA GTGCTTCAGG AGGAGAAAGA CAATCCCAAA CAAGGGACTT CACTGGAGGA 
ACAGGATCAA GACCAAGACC AGCTTTGCTG CCTCTTGACC TGCTTCTGAA AGTGCCACCC 
CACATGCTCA GGGCCCACAT TAAGGAAATA GAGGCTGAGT TAGTGACAGG GTGGCAGTCC 
CATAGCCTTC CTGCTGTGAT TCTTCGAAAT CTCAAAGATC ATGGGCCACA GATGGGCACA 
TTCTTGTGGC AAGCATCAGC AGGAATTGCT GTGTCCCAGA GGAAAGTGCG TCAGATCAGT 
GATCCTATTC AGCAGATATT AATTCAGCTG CACAAAATAA TCTATATCAC ACAGCTTCCT 
CCAGCTTTGC ACCACAATTT GAAAAGAAGG GTTATAGAGA GATTCAAGAA ATCCCTCTTC 
AGCCAGCAGA GTAACCCTTG TAATTTGAAA TCTGAAATTA AAAAGTTATC TCAGGGATCT 
CCAGAACCGA TTGAGCCCAA CTTTTTCACA GCAGATTACC ATTTATTACA TCGTTCATCC 
GGTGGAAACA GCCTGTCCCC AAATGACCCT ACAGGTTTAC CAACCAGCAT TGAATTGGAG 
GAAGGAATAA CATATGAACA GATGCAGACT GTGATTGAAG AAGTCCTTGA GGAAAGTGGC 
TATTACAATT TTACATCTAA CAGGTATCAT TCCTATCCAT GGGGGACCAA GAATCACCCA 
ACCAAAAGAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NEK10 ( XM_017005767.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_016861256.1 Homo sapiens NIMA related kinase 10 (NEK10), transcript variant X12, mRNA.

Price & Availability↑

CloneID	OHu93835
Clone ID Related Accession (Same CDS sequence)	XM_017005767.1 , XM_024453373.1 , XM_017005767.2
Accession Version	XM_017005767.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3432bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	serine/threonine-protein kinase Nek10 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000003.12) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_017005767.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCTGATC AAGATAAAAA GGTGAAGACC ACAGAAAAAT CAACTGATAA ACAGCAAGAA 
ATCACCATCA GGGACTATTC AGATCTTAAA AGACTTCGGT GCCTTTTGAA CGTCCAATCA 
AGCAAACAAC AGCTTCCAGC CATTAACTTC GATAGTGCCC AAAATAGCAT GACGAAGTCT 
GAGCCCGCCA TCAGGGCGGG TGGACACAGA GCTCGGGGTC AGTGGCATGA ATCCACAGAA 
GCTGTTGAAC TTGAAAATTT TAGTATAAAC TACAAGAATG AGAGAAATTT CAGCAAACAT 
CCTCAGCGTA AACTATTTCA GGAGATCTTT ACCGCCTTGG TGAAAAATAG ACTCATAAGC 
AGAGAGTGGG TTAATCGAGC CCCATCTATT CATTTTCTGA GAGTGTTAAT CTGTCTGAGG 
CTACTAATGA GGGATCCATG TTATCAGGAA ATACTCCATA GCTTGGGTGG GATTGAAAAC 
CTAGCTCAGT ATATGGAGAT TGTAGCCAAT GAGTACCTCG GCTATGGAGA AGAGCAGCAC 
ACTGTGGACA AGCTGGTCAA CATGACATAT ATTTTTCAAA AACTTGCTGC AGTCAAAGAT 
CAAAGAGAAT GGGTCACCAC AAGTGGAGCC CACAAGACAT TAGTAAATTT ACTTGGTGCC 
CGAGATACTA ATGTTCTATT GGGTTCCCTT CTGGCTCTGG CTAGTTTAGC AGAAAGACTA 
ACAGCGGAGT TGCTGCGCCT ACTTTGTGCA GAGCCCCAGG TGAAAGAGCA GGTGAAGCTC 
TATGAGGGGA TACCGGTCCT CCTCAGTCTG CTCCACTCTG ACCACTTGAA GCTCCTCTGG 
AGCATTGTCT GGATTCTGGT ACAGGTTTGT GAGGACCCTG AGACCAGCGT GGAAATTCGC 
ATTTGGGGAG GCATCAAACA GCTTCTTCAT ATTTTACAAG GAGACAGAAA TTTTGTTTCT 
GATCACTCCT CCATTGGAAG CCTGTCCAGT GCAAATGCTG CAGGCCGAAT CCAGCAGCTT 
CATTTATCAG AAGACTTGAG CCCTAGGGAA ATACAAGAAA ATACTTTCTC ACTTCAAGCA 
GCCTGCTGTG CTGCCCTCAC TGAGCTGGTG CTCAATGACA CCAATGCCCA CCAGGTGGTT 
CAGGAAAATG GTGTATATAC AATAGCAAAA TTAATTTTAC CAAATAAGCA AAAGAATGCA 
GCAAAAAGTA ATCTATTACA GTGTTATGCT TTCAGAGCCT TGAGATTTCT CTTCAGTATG 
GAAAGAAACA GACCACTCTT TAAAAGACTT TTCCCCACAG ACTTGTTTGA GATCTTCATT 
GACATAGGGC ATTATGTACG TGATATCAGT GCTTATGAAG AATTGGTATC CAAGCTGAAT 
TTATTAGTGG AGGATGAACT GAAGCAAATT GCTGAAAATA TTGAAAGCAT TAATCAGAAC 
AAAGCTCCTT TGAAATATAT AGGCAACTAT GCAATTTTGG ATCATCTTGG AAGTGGAGCT 
TTTGGCTGTG TTTACAAGGT TAGAAAGCAT AGTGGTCAAA ATCTTTTAGC AATGAAAGAG 
GTCAATTTAC ATAACCCAGC ATTTGGAAAG GATAAGAAAG ATCGAGACAG CAGCGTAAGG 
AATATTGTTT CTGAATTAAC AATAATTAAA GAGCAGCTTT ATCATCCCAA CATTGTACGT 
TATTACAAAA CATTTCTGGA AAATGATAGG TTGTACATAG TTATGGAGCT GATAGAAGGA 
GCCCCGCTTG GAGAGCATTT CAGTTCTTTG AAGGAAAAAC ATCACCATTT TACTGAAGAA 
AGACTATGGA AAATATTTAT ACAGCTGTGC TTAGCTCTTC GATACTTACA CAAGGAGAAG 
AGGATTGTCC ATAGAGATCT GACACCAAAC AACATTATGT TGGGGGATAA GGACAAAGTA 
ACCGTTACTG ACTTTGGCCT GGCAAAGCAA AAACAAGAAA ACAGTAAACT CACCTCTGTG 
GTTGGAACAA TCCTGTATTC TTGCCCCGAG GTACTGAAGA GTGAGCCGTA TGGGGAGAAG 
GCTGATGTCT GGGCAGTAGG CTGCATCCTT TATCAGATGG CGACTTTGAG TCCCCCCTTC 
TACAGCACTA ACATGCTGTC CTTGGCTACA AAAATAGTGG AGGCGGTATA TGAACCAGTC 
CCAGAAGGTA TCTACTCTGA AAAAGTAACA GACACCATCA GCAGGTGCCT CACTCCTGAT 
GCGGAAGCTC GTCCAGATAT TGTAGAAGTC AGTTCGATGA TATCAGATGT CATGATGAAA 
TATTTAGACA ACTTATCTAC ATCCCAGTTG TCCTTGGAAA AGAAGCTAGA ACGGGAACGA 
AGACGCACAC AAAGGTATTT TATGGAAGCC AACCGGAACA CCGTCACATG TCACCATGAG 
CTGGCTGTTC TATCTCACGA GACCTTTGAG AAGGCAAGTT TGAGTAGCAG CAGCAGTGGA 
GCAGCCAGCC TGAAAAGTGA ACTTTCAGAA AGCGCAGACC TGCCCCCTGA AGGCTTCCAG 
GCCTCCTATG GTAAAGACGA AGACAGGGCC TGTGACGAAA TCCTGTCAGA TGATAACTTC 
AACCTGGAAA ATGCTGAGAA AGATACATAT TCAGAGGTAG ATGATGAATT GGACATTTCG 
GATAACTCCA GCAGCTCCAG TTCAAGCCCT CTGAAAGAAT CTACATTCAA CATTTTAAAG 
AGAAGTTTTA GTGCTTCAGG AGGAGAAAGA CAATCCCAAA CAAGGGACTT CACTGGAGGA 
ACAGGATCAA GACCAAGACC AGCTTTGCTG CCTCTTGACC TGCTTCTGAA AGTGCCACCC 
CACATGCTCA GGGCCCACAT TAAGGAAATA GAGGCTGAGT TAGTGACAGG GTGGCAGTCC 
CATAGCCTTC CTGCTGTGAT TCTTCGAAAT CTCAAAGATC ATGGGCCACA GATGGGCACA 
TTCTTGTGGC AAGCATCAGC AGGAATTGCT GTGTCCCAGA GGAAAGTGCG TCAGATCAGT 
GATCCTATTC AGCAGATATT AATTCAGCTG CACAAAATAA TCTATATCAC ACAGCTTCCT 
CCAGCTTTGC ACCACAATTT GAAAAGAAGG GTTATAGAGA GATTCAAGAA ATCCCTCTTC 
AGCCAGCAGA GTAACCCTTG TAATTTGAAA TCTGAAATTA AAAAGTTATC TCAGGGATCT 
CCAGAACCGA TTGAGCCCAA CTTTTTCACA GCAGATTACC ATTTATTACA TCGTTCATCC 
GGTGGAAACA GCCTGTCCCC AAATGACCCT ACAGGTTTAC CAACCAGCAT TGAATTGGAG 
GAAGGAATAA CATATGAACA GATGCAGACT GTGATTGAAG AAGTCCTTGA GGAAAGTGGC 
TATTACAATT TTACATCTAA CAGGTATCAT TCCTATCCAT GGGGGACCAA GAATCACCCA 
ACCAAAAGAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016861256.1
CDS	274..3705
Translation	MPDQDKKVKTTEKSTDKQQEITIRDYSDLKRLRCLLNVQSSKQQLPAINFDSAQNSMTKSEPAIRAGGHRARGQWHESTEAVELENFSINYKNERNFSKHPQRKLFQEIFTALVKNRLISREWVNRAPSIHFLRVLICLRLLMRDPCYQEILHSLGGIENLAQYMEIVANEYLGYGEEQHTVDKLVNMTYIFQKLAAVKDQREWVTTSGAHKTLVNLLGARDTNVLLGSLLALASLAERLTAELLRLLCAEPQVKEQVKLYEGIPVLLSLLHSDHLKLLWSIVWILVQVCEDPETSVEIRIWGGIKQLLHILQGDRNFVSDHSSIGSLSSANAAGRIQQLHLSEDLSPREIQENTFSLQAACCAALTELVLNDTNAHQVVQENGVYTIAKLILPNKQKNAAKSNLLQCYAFRALRFLFSMERNRPLFKRLFPTDLFEIFIDIGHYVRDISAYEELVSKLNLLVEDELKQIAENIESINQNKAPLKYIGNYAILDHLGSGAFGCVYKVRKHSGQNLLAMKEVNLHNPAFGKDKKDRDSSVRNIVSELTIIKEQLYHPNIVRYYKTFLENDRLYIVMELIEGAPLGEHFSSLKEKHHHFTEERLWKIFIQLCLALRYLHKEKRIVHRDLTPNNIMLGDKDKVTVTDFGLAKQKQENSKLTSVVGTILYSCPEVLKSEPYGEKADVWAVGCILYQMATLSPPFYSTNMLSLATKIVEAVYEPVPEGIYSEKVTDTISRCLTPDAEARPDIVEVSSMISDVMMKYLDNLSTSQLSLEKKLERERRRTQRYFMEANRNTVTCHHELAVLSHETFEKASLSSSSSGAASLKSELSESADLPPEGFQASYGKDEDRACDEILSDDNFNLENAEKDTYSEVDDELDISDNSSSSSSSPLKESTFNILKRSFSASGGERQSQTRDFTGGTGSRPRPALLPLDLLLKVPPHMLRAHIKEIEAELVTGWQSHSLPAVILRNLKDHGPQMGTFLWQASAGIAVSQRKVRQISDPIQQILIQLHKIIYITQLPPALHHNLKRRVIERFKKSLFSQQSNPCNLKSEIKKLSQGSPEPIEPNFFTADYHLLHRSSGGNSLSPNDPTGLPTSIELEEGITYEQMQTVIEEVLEESGYYNFTSNRYHSYPWGTKNHPTKR

Target ORF information:

RefSeq Version	XM_017005767.2
Organism	Homo sapiens(human)
Definition	Homo sapiens NIMA related kinase 10 (NEK10), transcript variant X12, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017005767.2

ORF Insert Sequence:

ATGCCTGATC AAGATAAAAA GGTGAAGACC ACAGAAAAAT CAACTGATAA ACAGCAAGAA 
ATCACCATCA GGGACTATTC AGATCTTAAA AGACTTCGGT GCCTTTTGAA CGTCCAATCA 
AGCAAACAAC AGCTTCCAGC CATTAACTTC GATAGTGCCC AAAATAGCAT GACGAAGTCT 
GAGCCCGCCA TCAGGGCGGG TGGACACAGA GCTCGGGGTC AGTGGCATGA ATCCACAGAA 
GCTGTTGAAC TTGAAAATTT TAGTATAAAC TACAAGAATG AGAGAAATTT CAGCAAACAT 
CCTCAGCGTA AACTATTTCA GGAGATCTTT ACCGCCTTGG TGAAAAATAG ACTCATAAGC 
AGAGAGTGGG TTAATCGAGC CCCATCTATT CATTTTCTGA GAGTGTTAAT CTGTCTGAGG 
CTACTAATGA GGGATCCATG TTATCAGGAA ATACTCCATA GCTTGGGTGG GATTGAAAAC 
CTAGCTCAGT ATATGGAGAT TGTAGCCAAT GAGTACCTCG GCTATGGAGA AGAGCAGCAC 
ACTGTGGACA AGCTGGTCAA CATGACATAT ATTTTTCAAA AACTTGCTGC AGTCAAAGAT 
CAAAGAGAAT GGGTCACCAC AAGTGGAGCC CACAAGACAT TAGTAAATTT ACTTGGTGCC 
CGAGATACTA ATGTTCTATT GGGTTCCCTT CTGGCTCTGG CTAGTTTAGC AGAAAGACTA 
ACAGCGGAGT TGCTGCGCCT ACTTTGTGCA GAGCCCCAGG TGAAAGAGCA GGTGAAGCTC 
TATGAGGGGA TACCGGTCCT CCTCAGTCTG CTCCACTCTG ACCACTTGAA GCTCCTCTGG 
AGCATTGTCT GGATTCTGGT ACAGGTTTGT GAGGACCCTG AGACCAGCGT GGAAATTCGC 
ATTTGGGGAG GCATCAAACA GCTTCTTCAT ATTTTACAAG GAGACAGAAA TTTTGTTTCT 
GATCACTCCT CCATTGGAAG CCTGTCCAGT GCAAATGCTG CAGGCCGAAT CCAGCAGCTT 
CATTTATCAG AAGACTTGAG CCCTAGGGAA ATACAAGAAA ATACTTTCTC ACTTCAAGCA 
GCCTGCTGTG CTGCCCTCAC TGAGCTGGTG CTCAATGACA CCAATGCCCA CCAGGTGGTT 
CAGGAAAATG GTGTATATAC AATAGCAAAA TTAATTTTAC CAAATAAGCA AAAGAATGCA 
GCAAAAAGTA ATCTATTACA GTGTTATGCT TTCAGAGCCT TGAGATTTCT CTTCAGTATG 
GAAAGAAACA GACCACTCTT TAAAAGACTT TTCCCCACAG ACTTGTTTGA GATCTTCATT 
GACATAGGGC ATTATGTACG TGATATCAGT GCTTATGAAG AATTGGTATC CAAGCTGAAT 
TTATTAGTGG AGGATGAACT GAAGCAAATT GCTGAAAATA TTGAAAGCAT TAATCAGAAC 
AAAGCTCCTT TGAAATATAT AGGCAACTAT GCAATTTTGG ATCATCTTGG AAGTGGAGCT 
TTTGGCTGTG TTTACAAGGT TAGAAAGCAT AGTGGTCAAA ATCTTTTAGC AATGAAAGAG 
GTCAATTTAC ATAACCCAGC ATTTGGAAAG GATAAGAAAG ATCGAGACAG CAGCGTAAGG 
AATATTGTTT CTGAATTAAC AATAATTAAA GAGCAGCTTT ATCATCCCAA CATTGTACGT 
TATTACAAAA CATTTCTGGA AAATGATAGG TTGTACATAG TTATGGAGCT GATAGAAGGA 
GCCCCGCTTG GAGAGCATTT CAGTTCTTTG AAGGAAAAAC ATCACCATTT TACTGAAGAA 
AGACTATGGA AAATATTTAT ACAGCTGTGC TTAGCTCTTC GATACTTACA CAAGGAGAAG 
AGGATTGTCC ATAGAGATCT GACACCAAAC AACATTATGT TGGGGGATAA GGACAAAGTA 
ACCGTTACTG ACTTTGGCCT GGCAAAGCAA AAACAAGAAA ACAGTAAACT CACCTCTGTG 
GTTGGAACAA TCCTGTATTC TTGCCCCGAG GTACTGAAGA GTGAGCCGTA TGGGGAGAAG 
GCTGATGTCT GGGCAGTAGG CTGCATCCTT TATCAGATGG CGACTTTGAG TCCCCCCTTC 
TACAGCACTA ACATGCTGTC CTTGGCTACA AAAATAGTGG AGGCGGTATA TGAACCAGTC 
CCAGAAGGTA TCTACTCTGA AAAAGTAACA GACACCATCA GCAGGTGCCT CACTCCTGAT 
GCGGAAGCTC GTCCAGATAT TGTAGAAGTC AGTTCGATGA TATCAGATGT CATGATGAAA 
TATTTAGACA ACTTATCTAC ATCCCAGTTG TCCTTGGAAA AGAAGCTAGA ACGGGAACGA 
AGACGCACAC AAAGGTATTT TATGGAAGCC AACCGGAACA CCGTCACATG TCACCATGAG 
CTGGCTGTTC TATCTCACGA GACCTTTGAG AAGGCAAGTT TGAGTAGCAG CAGCAGTGGA 
GCAGCCAGCC TGAAAAGTGA ACTTTCAGAA AGCGCAGACC TGCCCCCTGA AGGCTTCCAG 
GCCTCCTATG GTAAAGACGA AGACAGGGCC TGTGACGAAA TCCTGTCAGA TGATAACTTC 
AACCTGGAAA ATGCTGAGAA AGATACATAT TCAGAGGTAG ATGATGAATT GGACATTTCG 
GATAACTCCA GCAGCTCCAG TTCAAGCCCT CTGAAAGAAT CTACATTCAA CATTTTAAAG 
AGAAGTTTTA GTGCTTCAGG AGGAGAAAGA CAATCCCAAA CAAGGGACTT CACTGGAGGA 
ACAGGATCAA GACCAAGACC AGCTTTGCTG CCTCTTGACC TGCTTCTGAA AGTGCCACCC 
CACATGCTCA GGGCCCACAT TAAGGAAATA GAGGCTGAGT TAGTGACAGG GTGGCAGTCC 
CATAGCCTTC CTGCTGTGAT TCTTCGAAAT CTCAAAGATC ATGGGCCACA GATGGGCACA 
TTCTTGTGGC AAGCATCAGC AGGAATTGCT GTGTCCCAGA GGAAAGTGCG TCAGATCAGT 
GATCCTATTC AGCAGATATT AATTCAGCTG CACAAAATAA TCTATATCAC ACAGCTTCCT 
CCAGCTTTGC ACCACAATTT GAAAAGAAGG GTTATAGAGA GATTCAAGAA ATCCCTCTTC 
AGCCAGCAGA GTAACCCTTG TAATTTGAAA TCTGAAATTA AAAAGTTATC TCAGGGATCT 
CCAGAACCGA TTGAGCCCAA CTTTTTCACA GCAGATTACC ATTTATTACA TCGTTCATCC 
GGTGGAAACA GCCTGTCCCC AAATGACCCT ACAGGTTTAC CAACCAGCAT TGAATTGGAG 
GAAGGAATAA CATATGAACA GATGCAGACT GTGATTGAAG AAGTCCTTGA GGAAAGTGGC 
TATTACAATT TTACATCTAA CAGGTATCAT TCCTATCCAT GGGGGACCAA GAATCACCCA 
ACCAAAAGAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NEK10 ( XM_017005768.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016861257.1 Homo sapiens NIMA related kinase 10 (NEK10), transcript variant X13, mRNA.

Price & Availability↑

CloneID	OHu93836
Clone ID Related Accession (Same CDS sequence)	XM_017005768.1
Accession Version	XM_017005768.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3381bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	serine/threonine-protein kinase Nek10 isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000003.12) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCTGATC AAGATAAAAA GGTGAAGACC ACAGAAAAAT CAACTGATAA ACAGCAAGAA 
ATCACCATCA GGGACTATTC AGATCTTAAA AGACTTCGGT GCCTTTTGAA CGTCCAATCA 
AGCAAACAAC AGCTTCCAGC CATTAACTTC GATAGTGCCC AAAATAGCAT GACGAAGTCT 
GAGCCCGCCA TCAGGGCGGG TGGACACAGA GCTCGGGGTC AGTGGCATGA ATCCACAGAA 
GCTGTTGAAC TTGAAAATTT TAGTATAAAC TACAAGAATG AGAGAAATTT CAGCAAACAT 
CCTCAGCGTA AACTATTTCA GGAGATCTTT ACCGCCTTGG TGAAAAATAG ACTCATAAGC 
AGAGAGTGGG TTAATCGAGC CCCATCTATT CATTTTCTGA GAGTGTTAAT CTGTCTGAGG 
CTACTAATGA GGGATCCATG TTATCAGGAA ATACTCCATA GCTTGGGTGG GATTGAAAAC 
CTAGCTCAGT ATATGGAGAT TGTAGCCAAT GAGTACCTCG GCTATGGAGA AGAGCAGCAC 
ACTGTGGACA AGCTGGTCAA CATGACATAT ATTTTTCAAA AACTTGCTGC AGTCAAAGAT 
CAAAGAGAAT GGGTCACCAC AAGTGGAGCC CACAAGACAT TAGTAAATTT ACTTGGTGCC 
CGAGATACTA ATGTTCTATT GGGTTCCCTT CTGGCTCTGG CTAGTTTAGC AGAAAGTCAA 
GAATGTAGGG AGAAGATAAG TGAACTCAAC ATTGTAGAAA ATCTGTTGAT GATTTTACAT 
GAATATGACT TGCTTTCTAA AAGACTAACA GCGGAGTTGC TGCGCCTACT TTGTGCAGAG 
CCCCAGGTGA AAGAGCAGGT GAAGCTCTAT GAGGGGATAC CGGTCCTCCT CAGTCTGCTC 
CACTCTGACC ACTTGAAGCT CCTCTGGAGC ATTGTCTGGA TTCTGGTACA GGTTTGTGAG 
GACCCTGAGA CCAGCGTGGA AATTCGCATT TGGGGAGGCA TCAAACAGCT TCTTCATATT 
TTACAAGGAG ACAGAAATTT TGTTTCTGAT CACTCCTCCA TTGGAAGCCT GTCCAGTGCA 
AATGCTGCAG GCCGAATCCA GCAGCTTCAT TTATCAGAAG ACTTGAGCCC TAGGGAAATA 
CAAGAAAATA CTTTCTCACT TCAAGCAGCC TGCTGTGCTG CCCTCACTGA GCTGGTGCTC 
AATGACACCA ATGCCCACCA GGTGGTTCAG GAAAATGGTG TATATACAAT AGCAAAATTA 
ATTTTACCAA ATAAGCAAAA GAATGCAGCA AAAAGTAATC TATTACAGTG TTATGCTTTC 
AGAGCCTTGA GATTTCTCTT CAGTATGGAA AGAAACAGAC CACTCTTTAA AAGACTTTTC 
CCCACAGACT TGTTTGAGAT CTTCATTGAC ATAGGGCATT ATGTACGTGA TATCAGTGCT 
TATGAAGAAT TGGTATCCAA GCTGAATTTA TTAGTGGAGG ATGAACTGAA GCAAATTGCT 
GAAAATATTG AAAGCATTAA TCAGAACAAA GCTCCTTTGA AATATATAGG CAACTATGCA 
ATTTTGGATC ATCTTGGAAG TGGAGCTTTT GGCTGTGTTT ACAAGCTTTA TCATCCCAAC 
ATTGTACGTT ATTACAAAAC ATTTCTGGAA AATGATAGGT TGTACATAGT TATGGAGCTG 
ATAGAAGGAG CCCCGCTTGG AGAGCATTTC AGTTCTTTGA AGGAAAAACA TCACCATTTT 
ACTGAAGAAA GACTATGGAA AATATTTATA CAGCTGTGCT TAGCTCTTCG ATACTTACAC 
AAGGAGAAGA GGATTGTCCA TAGAGATCTG ACACCAAACA ACATTATGTT GGGGGATAAG 
GACAAAGTAA CCGTTACTGA CTTTGGCCTG GCAAAGCAAA AACAAGAAAA CAGTAAACTC 
ACCTCTGTGG TTGGAACAAT CCTGTATTCT TGCCCCGAGG TACTGAAGAG TGAGCCGTAT 
GGGGAGAAGG CTGATGTCTG GGCAGTAGGC TGCATCCTTT ATCAGATGGC GACTTTGAGT 
CCCCCCTTCT ACAGCACTAA CATGCTGTCC TTGGCTACAA AAATAGTGGA GGCGGTATAT 
GAACCAGTCC CAGAAGGTAT CTACTCTGAA AAAGTAACAG ACACCATCAG CAGGTGCCTC 
ACTCCTGATG CGGAAGCTCG TCCAGATATT GTAGAAGTCA GTTCGATGAT ATCAGATGTC 
ATGATGAAAT ATTTAGACAA CTTATCTACA TCCCAGTTGT CCTTGGAAAA GAAGCTAGAA 
CGGGAACGAA GACGCACACA AAGGTATTTT ATGGAAGCCA ACCGGAACAC CGTCACATGT 
CACCATGAGC TGGCTGTTCT ATCTCACGAG ACCTTTGAGA AGGCAAGTTT GAGTAGCAGC 
AGCAGTGGAG CAGCCAGCCT GAAAAGTGAA CTTTCAGAAA GCGCAGACCT GCCCCCTGAA 
GGCTTCCAGG CCTCCTATGG TAAAGACGAA GACAGGGCCT GTGACGAAAT CCTGTCAGAT 
GATAACTTCA ACCTGGAAAA TGCTGAGAAA GATACATATT CAGAGGTAGA TGATGAATTG 
GACATTTCGG ATAACTCCAG CAGCTCCAGT TCAAGCCCTC TGAAAGAATC TACATTCAAC 
ATTTTAAAGA GAAGTTTTAG TGCTTCAGGA GGAGAAAGAC AATCCCAAAC AAGGGACTTC 
ACTGGAGGAA CAGGATCAAG ACCAAGACCA GCTTTGCTGC CTCTTGACCT GCTTCTGAAA 
GTGCCACCCC ACATGCTCAG GGCCCACATT AAGGAAATAG AGGCTGAGTT AGTGACAGGG 
TGGCAGTCCC ATAGCCTTCC TGCTGTGATT CTTCGAAATC TCAAAGATCA TGGGCCACAG 
ATGGGCACAT TCTTGTGGCA AGCATCAGCA GGAATTGCTG TGTCCCAGAG GAAAGTGCGT 
CAGATCAGTG ATCCTATTCA GCAGATATTA ATTCAGCTGC ACAAAATAAT CTATATCACA 
CAGCTTCCTC CAGCTTTGCA CCACAATTTG AAAAGAAGGG TTATAGAGAG ATTCAAGAAA 
TCCCTCTTCA GCCAGCAGAG TAACCCTTGT AATTTGAAAT CTGAAATTAA AAAGTTATCT 
CAGGGATCTC CAGAACCGAT TGAGCCCAAC TTTTTCACAG CAGATTACCA TTTATTACAT 
CGTTCATCCG GTGGAAACAG CCTGTCCCCA AATGACCCTA CAGGTTTACC AACCAGCATT 
GAATTGGAGG AAGGAATAAC ATATGAACAG ATGCAGACTG TGATTGAAGA AGTCCTTGAG 
GAAAGTGGCT ATTACAATTT TACATCTAAC AGGTATCATT CCTATCCATG GGGGACCAAG 
AATCACCCAA CCAAAAGATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016861257.1
CDS	275..3655
Translation	MPDQDKKVKTTEKSTDKQQEITIRDYSDLKRLRCLLNVQSSKQQLPAINFDSAQNSMTKSEPAIRAGGHRARGQWHESTEAVELENFSINYKNERNFSKHPQRKLFQEIFTALVKNRLISREWVNRAPSIHFLRVLICLRLLMRDPCYQEILHSLGGIENLAQYMEIVANEYLGYGEEQHTVDKLVNMTYIFQKLAAVKDQREWVTTSGAHKTLVNLLGARDTNVLLGSLLALASLAESQECREKISELNIVENLLMILHEYDLLSKRLTAELLRLLCAEPQVKEQVKLYEGIPVLLSLLHSDHLKLLWSIVWILVQVCEDPETSVEIRIWGGIKQLLHILQGDRNFVSDHSSIGSLSSANAAGRIQQLHLSEDLSPREIQENTFSLQAACCAALTELVLNDTNAHQVVQENGVYTIAKLILPNKQKNAAKSNLLQCYAFRALRFLFSMERNRPLFKRLFPTDLFEIFIDIGHYVRDISAYEELVSKLNLLVEDELKQIAENIESINQNKAPLKYIGNYAILDHLGSGAFGCVYKLYHPNIVRYYKTFLENDRLYIVMELIEGAPLGEHFSSLKEKHHHFTEERLWKIFIQLCLALRYLHKEKRIVHRDLTPNNIMLGDKDKVTVTDFGLAKQKQENSKLTSVVGTILYSCPEVLKSEPYGEKADVWAVGCILYQMATLSPPFYSTNMLSLATKIVEAVYEPVPEGIYSEKVTDTISRCLTPDAEARPDIVEVSSMISDVMMKYLDNLSTSQLSLEKKLERERRRTQRYFMEANRNTVTCHHELAVLSHETFEKASLSSSSSGAASLKSELSESADLPPEGFQASYGKDEDRACDEILSDDNFNLENAEKDTYSEVDDELDISDNSSSSSSSPLKESTFNILKRSFSASGGERQSQTRDFTGGTGSRPRPALLPLDLLLKVPPHMLRAHIKEIEAELVTGWQSHSLPAVILRNLKDHGPQMGTFLWQASAGIAVSQRKVRQISDPIQQILIQLHKIIYITQLPPALHHNLKRRVIERFKKSLFSQQSNPCNLKSEIKKLSQGSPEPIEPNFFTADYHLLHRSSGGNSLSPNDPTGLPTSIELEEGITYEQMQTVIEEVLEESGYYNFTSNRYHSYPWGTKNHPTKR

Target ORF information:

RefSeq Version	XM_017005768.1
Organism	Homo sapiens(human)
Definition	Homo sapiens NIMA related kinase 10 (NEK10), transcript variant X13, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017005768.1

ORF Insert Sequence:

ATGCCTGATC AAGATAAAAA GGTGAAGACC ACAGAAAAAT CAACTGATAA ACAGCAAGAA 
ATCACCATCA GGGACTATTC AGATCTTAAA AGACTTCGGT GCCTTTTGAA CGTCCAATCA 
AGCAAACAAC AGCTTCCAGC CATTAACTTC GATAGTGCCC AAAATAGCAT GACGAAGTCT 
GAGCCCGCCA TCAGGGCGGG TGGACACAGA GCTCGGGGTC AGTGGCATGA ATCCACAGAA 
GCTGTTGAAC TTGAAAATTT TAGTATAAAC TACAAGAATG AGAGAAATTT CAGCAAACAT 
CCTCAGCGTA AACTATTTCA GGAGATCTTT ACCGCCTTGG TGAAAAATAG ACTCATAAGC 
AGAGAGTGGG TTAATCGAGC CCCATCTATT CATTTTCTGA GAGTGTTAAT CTGTCTGAGG 
CTACTAATGA GGGATCCATG TTATCAGGAA ATACTCCATA GCTTGGGTGG GATTGAAAAC 
CTAGCTCAGT ATATGGAGAT TGTAGCCAAT GAGTACCTCG GCTATGGAGA AGAGCAGCAC 
ACTGTGGACA AGCTGGTCAA CATGACATAT ATTTTTCAAA AACTTGCTGC AGTCAAAGAT 
CAAAGAGAAT GGGTCACCAC AAGTGGAGCC CACAAGACAT TAGTAAATTT ACTTGGTGCC 
CGAGATACTA ATGTTCTATT GGGTTCCCTT CTGGCTCTGG CTAGTTTAGC AGAAAGTCAA 
GAATGTAGGG AGAAGATAAG TGAACTCAAC ATTGTAGAAA ATCTGTTGAT GATTTTACAT 
GAATATGACT TGCTTTCTAA AAGACTAACA GCGGAGTTGC TGCGCCTACT TTGTGCAGAG 
CCCCAGGTGA AAGAGCAGGT GAAGCTCTAT GAGGGGATAC CGGTCCTCCT CAGTCTGCTC 
CACTCTGACC ACTTGAAGCT CCTCTGGAGC ATTGTCTGGA TTCTGGTACA GGTTTGTGAG 
GACCCTGAGA CCAGCGTGGA AATTCGCATT TGGGGAGGCA TCAAACAGCT TCTTCATATT 
TTACAAGGAG ACAGAAATTT TGTTTCTGAT CACTCCTCCA TTGGAAGCCT GTCCAGTGCA 
AATGCTGCAG GCCGAATCCA GCAGCTTCAT TTATCAGAAG ACTTGAGCCC TAGGGAAATA 
CAAGAAAATA CTTTCTCACT TCAAGCAGCC TGCTGTGCTG CCCTCACTGA GCTGGTGCTC 
AATGACACCA ATGCCCACCA GGTGGTTCAG GAAAATGGTG TATATACAAT AGCAAAATTA 
ATTTTACCAA ATAAGCAAAA GAATGCAGCA AAAAGTAATC TATTACAGTG TTATGCTTTC 
AGAGCCTTGA GATTTCTCTT CAGTATGGAA AGAAACAGAC CACTCTTTAA AAGACTTTTC 
CCCACAGACT TGTTTGAGAT CTTCATTGAC ATAGGGCATT ATGTACGTGA TATCAGTGCT 
TATGAAGAAT TGGTATCCAA GCTGAATTTA TTAGTGGAGG ATGAACTGAA GCAAATTGCT 
GAAAATATTG AAAGCATTAA TCAGAACAAA GCTCCTTTGA AATATATAGG CAACTATGCA 
ATTTTGGATC ATCTTGGAAG TGGAGCTTTT GGCTGTGTTT ACAAGCTTTA TCATCCCAAC 
ATTGTACGTT ATTACAAAAC ATTTCTGGAA AATGATAGGT TGTACATAGT TATGGAGCTG 
ATAGAAGGAG CCCCGCTTGG AGAGCATTTC AGTTCTTTGA AGGAAAAACA TCACCATTTT 
ACTGAAGAAA GACTATGGAA AATATTTATA CAGCTGTGCT TAGCTCTTCG ATACTTACAC 
AAGGAGAAGA GGATTGTCCA TAGAGATCTG ACACCAAACA ACATTATGTT GGGGGATAAG 
GACAAAGTAA CCGTTACTGA CTTTGGCCTG GCAAAGCAAA AACAAGAAAA CAGTAAACTC 
ACCTCTGTGG TTGGAACAAT CCTGTATTCT TGCCCCGAGG TACTGAAGAG TGAGCCGTAT 
GGGGAGAAGG CTGATGTCTG GGCAGTAGGC TGCATCCTTT ATCAGATGGC GACTTTGAGT 
CCCCCCTTCT ACAGCACTAA CATGCTGTCC TTGGCTACAA AAATAGTGGA GGCGGTATAT 
GAACCAGTCC CAGAAGGTAT CTACTCTGAA AAAGTAACAG ACACCATCAG CAGGTGCCTC 
ACTCCTGATG CGGAAGCTCG TCCAGATATT GTAGAAGTCA GTTCGATGAT ATCAGATGTC 
ATGATGAAAT ATTTAGACAA CTTATCTACA TCCCAGTTGT CCTTGGAAAA GAAGCTAGAA 
CGGGAACGAA GACGCACACA AAGGTATTTT ATGGAAGCCA ACCGGAACAC CGTCACATGT 
CACCATGAGC TGGCTGTTCT ATCTCACGAG ACCTTTGAGA AGGCAAGTTT GAGTAGCAGC 
AGCAGTGGAG CAGCCAGCCT GAAAAGTGAA CTTTCAGAAA GCGCAGACCT GCCCCCTGAA 
GGCTTCCAGG CCTCCTATGG TAAAGACGAA GACAGGGCCT GTGACGAAAT CCTGTCAGAT 
GATAACTTCA ACCTGGAAAA TGCTGAGAAA GATACATATT CAGAGGTAGA TGATGAATTG 
GACATTTCGG ATAACTCCAG CAGCTCCAGT TCAAGCCCTC TGAAAGAATC TACATTCAAC 
ATTTTAAAGA GAAGTTTTAG TGCTTCAGGA GGAGAAAGAC AATCCCAAAC AAGGGACTTC 
ACTGGAGGAA CAGGATCAAG ACCAAGACCA GCTTTGCTGC CTCTTGACCT GCTTCTGAAA 
GTGCCACCCC ACATGCTCAG GGCCCACATT AAGGAAATAG AGGCTGAGTT AGTGACAGGG 
TGGCAGTCCC ATAGCCTTCC TGCTGTGATT CTTCGAAATC TCAAAGATCA TGGGCCACAG 
ATGGGCACAT TCTTGTGGCA AGCATCAGCA GGAATTGCTG TGTCCCAGAG GAAAGTGCGT 
CAGATCAGTG ATCCTATTCA GCAGATATTA ATTCAGCTGC ACAAAATAAT CTATATCACA 
CAGCTTCCTC CAGCTTTGCA CCACAATTTG AAAAGAAGGG TTATAGAGAG ATTCAAGAAA 
TCCCTCTTCA GCCAGCAGAG TAACCCTTGT AATTTGAAAT CTGAAATTAA AAAGTTATCT 
CAGGGATCTC CAGAACCGAT TGAGCCCAAC TTTTTCACAG CAGATTACCA TTTATTACAT 
CGTTCATCCG GTGGAAACAG CCTGTCCCCA AATGACCCTA CAGGTTTACC AACCAGCATT 
GAATTGGAGG AAGGAATAAC ATATGAACAG ATGCAGACTG TGATTGAAGA AGTCCTTGAG 
GAAAGTGGCT ATTACAATTT TACATCTAAC AGGTATCATT CCTATCCATG GGGGACCAAG 
AATCACCCAA CCAAAAGATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NEK10 ( XM_017005769.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016861258.1 Homo sapiens NIMA related kinase 10 (NEK10), transcript variant X14, mRNA.

Price & Availability↑

CloneID	OHu93837
Clone ID Related Accession (Same CDS sequence)	XM_017005769.1 , XM_017005770.1 , XM_017005770.2
Accession Version	XM_017005769.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3378bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	serine/threonine-protein kinase Nek10 isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000003.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCTGATC AAGATAAAAA GGTGAAGACC ACAGAAAAAT CAACTGATAA ACAGCAAGAA 
ATCACCATCA GGGACTATTC AGATCTTAAA AGACTTCGGT GCCTTTTGAA CGTCCAATCA 
AGCAAACAAC AGCTTCCAGC CATTAACTTC GATAGTGCCC AAAATAGCAT GACGAAGTCT 
GAGCCCGCCA TCAGGGCGGG TGGACACAGA GCTCGGGGTC AGTGGCATGA ATCCACAGAA 
GCTGTTGAAC TTGAAAATTT TAGTATAAAC TACAAGAATG AGAGAAATTT CAGCAAACAT 
CCTCAGCGTA AACTATTTCA GGAGATCTTT ACCGCCTTGG TGAAAAATAG ACTCATAAGC 
AGAGAGTGGG TTAATCGAGC CCCATCTATT CATTTTCTGA GAGTGTTAAT CTGTCTGAGG 
CTACTAATGA GGGATCCATG TTATCAGGAA ATACTCCATA GCTTGGGTGG GATTGAAAAC 
CTAGCTCAGT ATATGGAGAT TGTAGCCAAT GAGTACCTCG GCTATGGAGA AGAGCAGCAC 
ACTGTGGACA AGCTGGTCAA CATGACATAT ATTTTTCAAA AACTTGCTGC AGTCAAAGAT 
CAAAGAGAAT GGGTCACCAC AAGTGGAGCC CACAAGACAT TAGTAAATTT ACTTGGTGCC 
CGAGATACTA ATGTTCTATT GGGTTCCCTT CTGGCTCTGG CTAGTTTAGC AGAAAGTCAA 
GAATGTAGGG AGAAGATAAG TGAACTCAAC ATTGTAGAAA ATCTGTTGAT GATTTTACAT 
GAATATGACT TGCTTTCTAA AAGACTAACA GCGGAGTTGC TGCGCCTACT TTGTGCAGAG 
CCCCAGGTGA AAGAGCAGGT GAAGCTCTAT GAGGGGATAC CGGTCCTCCT CAGTCTGCTC 
CACTCTGACC ACTTGAAGCT CCTCTGGAGC ATTGTCTGGA TTCTGGTACA GGTTTGTGAG 
GACCCTGAGA CCAGCGTGGA AATTCGCATT TGGGGAGGCA TCAAACAGCT TCTTCATATT 
TTACAAGGAG ACAGAAATTT TGTTTCTGAT CACTCCTCCA TTGGAAGCCT GTCCAGTGCA 
AATGCTGCAG GCCGAATCCA GCAGCTTCAT TTATCAGAAG ACTTGAGCCC TAGGGAAATA 
CAAGAAAATA CTTTCTCACT TCAAGCAGCC TGCTGTGCTG CCCTCACTGA GCTGGTGCTC 
AATGACACCA ATGCCCACCA GGTGGTTCAG GAAAATGGTG TATATACAAT AGCAAAATTA 
ATTTTACCAA ATAAGCAAAA GAATGCAGCA AAAAGTAATC TATTACAGTG TTATGCTTTC 
AGAGCCTTGA GATTTCTCTT CAGTATGGAA AGAAACAGAC CACTCTTTAA AAGACTTTTC 
CCCACAGACT TGTTTGAGAT CTTCATTGAC ATAGGGCATT ATGTACGTGA TATCAGTGCT 
TATGAAGAAT TGGTATCCAA GCTGAATTTA TTAGTGGAGG ATGAACTGAA GCAAATTGCT 
GAAAATATTG AAAGCATTAA TCAGAACAAA GCTCCTTTGA AATATATAGG CAACTATGCA 
ATTTTGGATC ATCTTGGAAG TGGAGCTTTT GGCTGTGTTT ACAAGGTTAG AAAGCATAGT 
GGTCAAAATC TTTTAGCAAT GAAAGAGGTC AATTTACATA ACCCAGCATT TGGAAAGGAT 
AAGAAAGATC GAGACAGCAG CGTAAGGAAT ATTGTTTCTG AATTAACAAT AATTAAAGAG 
CAGCTTTATC ATCCCAACAT TGTACGTTAT TACAAAACAT TTCTGGAAAA TGATAGGTTG 
TACATAGTTA TGGAGCTGAT AGAAGGAGCC CCGCTTGGAG AGCATTTCAG TTCTTTGAAG 
GAAAAACATC ACCATTTTAC TGAAGAAAGA CTATGGAAAA TATTTATACA GCTGTGCTTA 
GCTCTTCGAT ACTTACACAA GGAGAAGAGG ATTGTCCATA GAGATCTGAC ACCAAACAAC 
ATTATGTTGG GGGATAAGGA CAAAGTAACC GTTACTGACT TTGGCCTGGC AAAGCAAAAA 
CAAGAAAACA GTAAACTCAC CTCTGTGGTT GGAACAATCC TGTATTCTTG CCCCGAGGTA 
CTGAAGAGTG AGCCGTATGG GGAGAAGGCT GATGTCTGGG CAGTAGGCTG CATCCTTTAT 
CAGATGGCGA CTTTGAGTCC CCCCTTCTAC AGCACTAACA TGCTGTCCTT GGCTACAAAA 
ATAGTGGAGG CGGTATATGA ACCAGTCCCA GAAGGTATCT ACTCTGAAAA AGTAACAGAC 
ACCATCAGCA GGTGCCTCAC TCCTGATGCG GAAGCTCGTC CAGATATTGT AGAAGTCAGT 
TCGATGATAT CAGATGTCAT GATGAAATAT TTAGACAACT TATCTACATC CCAGTTGTCC 
TTGGAAAAGA AGCTAGAACG GGAACGAAGA CGCACACAAA GGTATTTTAT GGAAGCCAAC 
CGGAACACCG TCACATGTCA CCATGAGCTG GCTGTTCTAT CTCACGAGAC CTTTGAGAAG 
GCAAGTTTGA GTAGCAGCAG CAGTGGAGCA GCCAGCCTGA AAAGTGAACT TTCAGAAAGC 
GCAGACCTGC CCCCTGAAGG CTTCCAGGCC TCCTATGGTA AAGACGAAGA CAGGGCCTGT 
GACGAAATCC TGTCAGATGA TAACTTCAAC CTGGAAAATG CTGAGAAAGA TACATATTCA 
GAGGTAGATG ATGAATTGGA CATTTCGGAT AACTCCAGCA GCTCCAGTTC AAGCCCTCTG 
AAAGAATCTA CATTCAACAT TTTAAAGAGA AGTTTTAGTG CTTCAGGAGG AGAAAGACAA 
TCCCAAACAA GGGACTTCAC TGGAGGAACA GGATCAAGAC CAAGACCAGG GCCACAGATG 
GGCACATTCT TGTGGCAAGC ATCAGCAGGA ATTGCTGTGT CCCAGAGGAA AGTGCGTCAG 
ATCAGTGATC CTATTCAGCA GATATTAATT CAGCTGCACA AAATAATCTA TATCACACAG 
CTTCCTCCAG CTTTGCACCA CAATTTGAAA AGAAGGGTTA TAGAGAGATT CAAGAAATCC 
CTCTTCAGCC AGCAGAGTAA CCCTTGTAAT TTGAAATCTG AAATTAAAAA GTTATCTCAG 
GGATCTCCAG AACCGATTGA GCCCAACTTT TTCACAGCAG ATTACCATTT ATTACATCGT 
TCATCCGGTG GAAACAGCCT GTCCCCAAAT GACCCTACAG GTTTACCAAC CAGCATTGAA 
TTGGAGGAAG GAATAACATA TGAACAGATG CAGACTGTGA TTGAAGAAGT CCTTGAGGAA 
AGTGGCTATT ACAATTTTAC ATCTAACAGG TATCATTCCT ATCCATGGGG GACCAAGAAT 
CACCCAACCA AAAGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016861258.1
CDS	275..3652
Translation	MPDQDKKVKTTEKSTDKQQEITIRDYSDLKRLRCLLNVQSSKQQLPAINFDSAQNSMTKSEPAIRAGGHRARGQWHESTEAVELENFSINYKNERNFSKHPQRKLFQEIFTALVKNRLISREWVNRAPSIHFLRVLICLRLLMRDPCYQEILHSLGGIENLAQYMEIVANEYLGYGEEQHTVDKLVNMTYIFQKLAAVKDQREWVTTSGAHKTLVNLLGARDTNVLLGSLLALASLAESQECREKISELNIVENLLMILHEYDLLSKRLTAELLRLLCAEPQVKEQVKLYEGIPVLLSLLHSDHLKLLWSIVWILVQVCEDPETSVEIRIWGGIKQLLHILQGDRNFVSDHSSIGSLSSANAAGRIQQLHLSEDLSPREIQENTFSLQAACCAALTELVLNDTNAHQVVQENGVYTIAKLILPNKQKNAAKSNLLQCYAFRALRFLFSMERNRPLFKRLFPTDLFEIFIDIGHYVRDISAYEELVSKLNLLVEDELKQIAENIESINQNKAPLKYIGNYAILDHLGSGAFGCVYKVRKHSGQNLLAMKEVNLHNPAFGKDKKDRDSSVRNIVSELTIIKEQLYHPNIVRYYKTFLENDRLYIVMELIEGAPLGEHFSSLKEKHHHFTEERLWKIFIQLCLALRYLHKEKRIVHRDLTPNNIMLGDKDKVTVTDFGLAKQKQENSKLTSVVGTILYSCPEVLKSEPYGEKADVWAVGCILYQMATLSPPFYSTNMLSLATKIVEAVYEPVPEGIYSEKVTDTISRCLTPDAEARPDIVEVSSMISDVMMKYLDNLSTSQLSLEKKLERERRRTQRYFMEANRNTVTCHHELAVLSHETFEKASLSSSSSGAASLKSELSESADLPPEGFQASYGKDEDRACDEILSDDNFNLENAEKDTYSEVDDELDISDNSSSSSSSPLKESTFNILKRSFSASGGERQSQTRDFTGGTGSRPRPGPQMGTFLWQASAGIAVSQRKVRQISDPIQQILIQLHKIIYITQLPPALHHNLKRRVIERFKKSLFSQQSNPCNLKSEIKKLSQGSPEPIEPNFFTADYHLLHRSSGGNSLSPNDPTGLPTSIELEEGITYEQMQTVIEEVLEESGYYNFTSNRYHSYPWGTKNHPTKR

Target ORF information:

RefSeq Version	XM_017005769.1
Organism	Homo sapiens(human)
Definition	Homo sapiens NIMA related kinase 10 (NEK10), transcript variant X14, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017005769.1

ORF Insert Sequence:

ATGCCTGATC AAGATAAAAA GGTGAAGACC ACAGAAAAAT CAACTGATAA ACAGCAAGAA 
ATCACCATCA GGGACTATTC AGATCTTAAA AGACTTCGGT GCCTTTTGAA CGTCCAATCA 
AGCAAACAAC AGCTTCCAGC CATTAACTTC GATAGTGCCC AAAATAGCAT GACGAAGTCT 
GAGCCCGCCA TCAGGGCGGG TGGACACAGA GCTCGGGGTC AGTGGCATGA ATCCACAGAA 
GCTGTTGAAC TTGAAAATTT TAGTATAAAC TACAAGAATG AGAGAAATTT CAGCAAACAT 
CCTCAGCGTA AACTATTTCA GGAGATCTTT ACCGCCTTGG TGAAAAATAG ACTCATAAGC 
AGAGAGTGGG TTAATCGAGC CCCATCTATT CATTTTCTGA GAGTGTTAAT CTGTCTGAGG 
CTACTAATGA GGGATCCATG TTATCAGGAA ATACTCCATA GCTTGGGTGG GATTGAAAAC 
CTAGCTCAGT ATATGGAGAT TGTAGCCAAT GAGTACCTCG GCTATGGAGA AGAGCAGCAC 
ACTGTGGACA AGCTGGTCAA CATGACATAT ATTTTTCAAA AACTTGCTGC AGTCAAAGAT 
CAAAGAGAAT GGGTCACCAC AAGTGGAGCC CACAAGACAT TAGTAAATTT ACTTGGTGCC 
CGAGATACTA ATGTTCTATT GGGTTCCCTT CTGGCTCTGG CTAGTTTAGC AGAAAGTCAA 
GAATGTAGGG AGAAGATAAG TGAACTCAAC ATTGTAGAAA ATCTGTTGAT GATTTTACAT 
GAATATGACT TGCTTTCTAA AAGACTAACA GCGGAGTTGC TGCGCCTACT TTGTGCAGAG 
CCCCAGGTGA AAGAGCAGGT GAAGCTCTAT GAGGGGATAC CGGTCCTCCT CAGTCTGCTC 
CACTCTGACC ACTTGAAGCT CCTCTGGAGC ATTGTCTGGA TTCTGGTACA GGTTTGTGAG 
GACCCTGAGA CCAGCGTGGA AATTCGCATT TGGGGAGGCA TCAAACAGCT TCTTCATATT 
TTACAAGGAG ACAGAAATTT TGTTTCTGAT CACTCCTCCA TTGGAAGCCT GTCCAGTGCA 
AATGCTGCAG GCCGAATCCA GCAGCTTCAT TTATCAGAAG ACTTGAGCCC TAGGGAAATA 
CAAGAAAATA CTTTCTCACT TCAAGCAGCC TGCTGTGCTG CCCTCACTGA GCTGGTGCTC 
AATGACACCA ATGCCCACCA GGTGGTTCAG GAAAATGGTG TATATACAAT AGCAAAATTA 
ATTTTACCAA ATAAGCAAAA GAATGCAGCA AAAAGTAATC TATTACAGTG TTATGCTTTC 
AGAGCCTTGA GATTTCTCTT CAGTATGGAA AGAAACAGAC CACTCTTTAA AAGACTTTTC 
CCCACAGACT TGTTTGAGAT CTTCATTGAC ATAGGGCATT ATGTACGTGA TATCAGTGCT 
TATGAAGAAT TGGTATCCAA GCTGAATTTA TTAGTGGAGG ATGAACTGAA GCAAATTGCT 
GAAAATATTG AAAGCATTAA TCAGAACAAA GCTCCTTTGA AATATATAGG CAACTATGCA 
ATTTTGGATC ATCTTGGAAG TGGAGCTTTT GGCTGTGTTT ACAAGGTTAG AAAGCATAGT 
GGTCAAAATC TTTTAGCAAT GAAAGAGGTC AATTTACATA ACCCAGCATT TGGAAAGGAT 
AAGAAAGATC GAGACAGCAG CGTAAGGAAT ATTGTTTCTG AATTAACAAT AATTAAAGAG 
CAGCTTTATC ATCCCAACAT TGTACGTTAT TACAAAACAT TTCTGGAAAA TGATAGGTTG 
TACATAGTTA TGGAGCTGAT AGAAGGAGCC CCGCTTGGAG AGCATTTCAG TTCTTTGAAG 
GAAAAACATC ACCATTTTAC TGAAGAAAGA CTATGGAAAA TATTTATACA GCTGTGCTTA 
GCTCTTCGAT ACTTACACAA GGAGAAGAGG ATTGTCCATA GAGATCTGAC ACCAAACAAC 
ATTATGTTGG GGGATAAGGA CAAAGTAACC GTTACTGACT TTGGCCTGGC AAAGCAAAAA 
CAAGAAAACA GTAAACTCAC CTCTGTGGTT GGAACAATCC TGTATTCTTG CCCCGAGGTA 
CTGAAGAGTG AGCCGTATGG GGAGAAGGCT GATGTCTGGG CAGTAGGCTG CATCCTTTAT 
CAGATGGCGA CTTTGAGTCC CCCCTTCTAC AGCACTAACA TGCTGTCCTT GGCTACAAAA 
ATAGTGGAGG CGGTATATGA ACCAGTCCCA GAAGGTATCT ACTCTGAAAA AGTAACAGAC 
ACCATCAGCA GGTGCCTCAC TCCTGATGCG GAAGCTCGTC CAGATATTGT AGAAGTCAGT 
TCGATGATAT CAGATGTCAT GATGAAATAT TTAGACAACT TATCTACATC CCAGTTGTCC 
TTGGAAAAGA AGCTAGAACG GGAACGAAGA CGCACACAAA GGTATTTTAT GGAAGCCAAC 
CGGAACACCG TCACATGTCA CCATGAGCTG GCTGTTCTAT CTCACGAGAC CTTTGAGAAG 
GCAAGTTTGA GTAGCAGCAG CAGTGGAGCA GCCAGCCTGA AAAGTGAACT TTCAGAAAGC 
GCAGACCTGC CCCCTGAAGG CTTCCAGGCC TCCTATGGTA AAGACGAAGA CAGGGCCTGT 
GACGAAATCC TGTCAGATGA TAACTTCAAC CTGGAAAATG CTGAGAAAGA TACATATTCA 
GAGGTAGATG ATGAATTGGA CATTTCGGAT AACTCCAGCA GCTCCAGTTC AAGCCCTCTG 
AAAGAATCTA CATTCAACAT TTTAAAGAGA AGTTTTAGTG CTTCAGGAGG AGAAAGACAA 
TCCCAAACAA GGGACTTCAC TGGAGGAACA GGATCAAGAC CAAGACCAGG GCCACAGATG 
GGCACATTCT TGTGGCAAGC ATCAGCAGGA ATTGCTGTGT CCCAGAGGAA AGTGCGTCAG 
ATCAGTGATC CTATTCAGCA GATATTAATT CAGCTGCACA AAATAATCTA TATCACACAG 
CTTCCTCCAG CTTTGCACCA CAATTTGAAA AGAAGGGTTA TAGAGAGATT CAAGAAATCC 
CTCTTCAGCC AGCAGAGTAA CCCTTGTAAT TTGAAATCTG AAATTAAAAA GTTATCTCAG 
GGATCTCCAG AACCGATTGA GCCCAACTTT TTCACAGCAG ATTACCATTT ATTACATCGT 
TCATCCGGTG GAAACAGCCT GTCCCCAAAT GACCCTACAG GTTTACCAAC CAGCATTGAA 
TTGGAGGAAG GAATAACATA TGAACAGATG CAGACTGTGA TTGAAGAAGT CCTTGAGGAA 
AGTGGCTATT ACAATTTTAC ATCTAACAGG TATCATTCCT ATCCATGGGG GACCAAGAAT 
CACCCAACCA AAAGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NEK10 ( XM_017005770.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016861259.1 Homo sapiens NIMA related kinase 10 (NEK10), transcript variant X14, mRNA.

Price & Availability↑

CloneID	OHu93837
Clone ID Related Accession (Same CDS sequence)	XM_017005769.1 , XM_017005770.1 , XM_017005770.2
Accession Version	XM_017005770.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3378bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	serine/threonine-protein kinase Nek10 isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000003.12) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCTGATC AAGATAAAAA GGTGAAGACC ACAGAAAAAT CAACTGATAA ACAGCAAGAA 
ATCACCATCA GGGACTATTC AGATCTTAAA AGACTTCGGT GCCTTTTGAA CGTCCAATCA 
AGCAAACAAC AGCTTCCAGC CATTAACTTC GATAGTGCCC AAAATAGCAT GACGAAGTCT 
GAGCCCGCCA TCAGGGCGGG TGGACACAGA GCTCGGGGTC AGTGGCATGA ATCCACAGAA 
GCTGTTGAAC TTGAAAATTT TAGTATAAAC TACAAGAATG AGAGAAATTT CAGCAAACAT 
CCTCAGCGTA AACTATTTCA GGAGATCTTT ACCGCCTTGG TGAAAAATAG ACTCATAAGC 
AGAGAGTGGG TTAATCGAGC CCCATCTATT CATTTTCTGA GAGTGTTAAT CTGTCTGAGG 
CTACTAATGA GGGATCCATG TTATCAGGAA ATACTCCATA GCTTGGGTGG GATTGAAAAC 
CTAGCTCAGT ATATGGAGAT TGTAGCCAAT GAGTACCTCG GCTATGGAGA AGAGCAGCAC 
ACTGTGGACA AGCTGGTCAA CATGACATAT ATTTTTCAAA AACTTGCTGC AGTCAAAGAT 
CAAAGAGAAT GGGTCACCAC AAGTGGAGCC CACAAGACAT TAGTAAATTT ACTTGGTGCC 
CGAGATACTA ATGTTCTATT GGGTTCCCTT CTGGCTCTGG CTAGTTTAGC AGAAAGTCAA 
GAATGTAGGG AGAAGATAAG TGAACTCAAC ATTGTAGAAA ATCTGTTGAT GATTTTACAT 
GAATATGACT TGCTTTCTAA AAGACTAACA GCGGAGTTGC TGCGCCTACT TTGTGCAGAG 
CCCCAGGTGA AAGAGCAGGT GAAGCTCTAT GAGGGGATAC CGGTCCTCCT CAGTCTGCTC 
CACTCTGACC ACTTGAAGCT CCTCTGGAGC ATTGTCTGGA TTCTGGTACA GGTTTGTGAG 
GACCCTGAGA CCAGCGTGGA AATTCGCATT TGGGGAGGCA TCAAACAGCT TCTTCATATT 
TTACAAGGAG ACAGAAATTT TGTTTCTGAT CACTCCTCCA TTGGAAGCCT GTCCAGTGCA 
AATGCTGCAG GCCGAATCCA GCAGCTTCAT TTATCAGAAG ACTTGAGCCC TAGGGAAATA 
CAAGAAAATA CTTTCTCACT TCAAGCAGCC TGCTGTGCTG CCCTCACTGA GCTGGTGCTC 
AATGACACCA ATGCCCACCA GGTGGTTCAG GAAAATGGTG TATATACAAT AGCAAAATTA 
ATTTTACCAA ATAAGCAAAA GAATGCAGCA AAAAGTAATC TATTACAGTG TTATGCTTTC 
AGAGCCTTGA GATTTCTCTT CAGTATGGAA AGAAACAGAC CACTCTTTAA AAGACTTTTC 
CCCACAGACT TGTTTGAGAT CTTCATTGAC ATAGGGCATT ATGTACGTGA TATCAGTGCT 
TATGAAGAAT TGGTATCCAA GCTGAATTTA TTAGTGGAGG ATGAACTGAA GCAAATTGCT 
GAAAATATTG AAAGCATTAA TCAGAACAAA GCTCCTTTGA AATATATAGG CAACTATGCA 
ATTTTGGATC ATCTTGGAAG TGGAGCTTTT GGCTGTGTTT ACAAGGTTAG AAAGCATAGT 
GGTCAAAATC TTTTAGCAAT GAAAGAGGTC AATTTACATA ACCCAGCATT TGGAAAGGAT 
AAGAAAGATC GAGACAGCAG CGTAAGGAAT ATTGTTTCTG AATTAACAAT AATTAAAGAG 
CAGCTTTATC ATCCCAACAT TGTACGTTAT TACAAAACAT TTCTGGAAAA TGATAGGTTG 
TACATAGTTA TGGAGCTGAT AGAAGGAGCC CCGCTTGGAG AGCATTTCAG TTCTTTGAAG 
GAAAAACATC ACCATTTTAC TGAAGAAAGA CTATGGAAAA TATTTATACA GCTGTGCTTA 
GCTCTTCGAT ACTTACACAA GGAGAAGAGG ATTGTCCATA GAGATCTGAC ACCAAACAAC 
ATTATGTTGG GGGATAAGGA CAAAGTAACC GTTACTGACT TTGGCCTGGC AAAGCAAAAA 
CAAGAAAACA GTAAACTCAC CTCTGTGGTT GGAACAATCC TGTATTCTTG CCCCGAGGTA 
CTGAAGAGTG AGCCGTATGG GGAGAAGGCT GATGTCTGGG CAGTAGGCTG CATCCTTTAT 
CAGATGGCGA CTTTGAGTCC CCCCTTCTAC AGCACTAACA TGCTGTCCTT GGCTACAAAA 
ATAGTGGAGG CGGTATATGA ACCAGTCCCA GAAGGTATCT ACTCTGAAAA AGTAACAGAC 
ACCATCAGCA GGTGCCTCAC TCCTGATGCG GAAGCTCGTC CAGATATTGT AGAAGTCAGT 
TCGATGATAT CAGATGTCAT GATGAAATAT TTAGACAACT TATCTACATC CCAGTTGTCC 
TTGGAAAAGA AGCTAGAACG GGAACGAAGA CGCACACAAA GGTATTTTAT GGAAGCCAAC 
CGGAACACCG TCACATGTCA CCATGAGCTG GCTGTTCTAT CTCACGAGAC CTTTGAGAAG 
GCAAGTTTGA GTAGCAGCAG CAGTGGAGCA GCCAGCCTGA AAAGTGAACT TTCAGAAAGC 
GCAGACCTGC CCCCTGAAGG CTTCCAGGCC TCCTATGGTA AAGACGAAGA CAGGGCCTGT 
GACGAAATCC TGTCAGATGA TAACTTCAAC CTGGAAAATG CTGAGAAAGA TACATATTCA 
GAGGTAGATG ATGAATTGGA CATTTCGGAT AACTCCAGCA GCTCCAGTTC AAGCCCTCTG 
AAAGAATCTA CATTCAACAT TTTAAAGAGA AGTTTTAGTG CTTCAGGAGG AGAAAGACAA 
TCCCAAACAA GGGACTTCAC TGGAGGAACA GGATCAAGAC CAAGACCAGG GCCACAGATG 
GGCACATTCT TGTGGCAAGC ATCAGCAGGA ATTGCTGTGT CCCAGAGGAA AGTGCGTCAG 
ATCAGTGATC CTATTCAGCA GATATTAATT CAGCTGCACA AAATAATCTA TATCACACAG 
CTTCCTCCAG CTTTGCACCA CAATTTGAAA AGAAGGGTTA TAGAGAGATT CAAGAAATCC 
CTCTTCAGCC AGCAGAGTAA CCCTTGTAAT TTGAAATCTG AAATTAAAAA GTTATCTCAG 
GGATCTCCAG AACCGATTGA GCCCAACTTT TTCACAGCAG ATTACCATTT ATTACATCGT 
TCATCCGGTG GAAACAGCCT GTCCCCAAAT GACCCTACAG GTTTACCAAC CAGCATTGAA 
TTGGAGGAAG GAATAACATA TGAACAGATG CAGACTGTGA TTGAAGAAGT CCTTGAGGAA 
AGTGGCTATT ACAATTTTAC ATCTAACAGG TATCATTCCT ATCCATGGGG GACCAAGAAT 
CACCCAACCA AAAGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016861259.1
CDS	227..3604
Translation	MPDQDKKVKTTEKSTDKQQEITIRDYSDLKRLRCLLNVQSSKQQLPAINFDSAQNSMTKSEPAIRAGGHRARGQWHESTEAVELENFSINYKNERNFSKHPQRKLFQEIFTALVKNRLISREWVNRAPSIHFLRVLICLRLLMRDPCYQEILHSLGGIENLAQYMEIVANEYLGYGEEQHTVDKLVNMTYIFQKLAAVKDQREWVTTSGAHKTLVNLLGARDTNVLLGSLLALASLAESQECREKISELNIVENLLMILHEYDLLSKRLTAELLRLLCAEPQVKEQVKLYEGIPVLLSLLHSDHLKLLWSIVWILVQVCEDPETSVEIRIWGGIKQLLHILQGDRNFVSDHSSIGSLSSANAAGRIQQLHLSEDLSPREIQENTFSLQAACCAALTELVLNDTNAHQVVQENGVYTIAKLILPNKQKNAAKSNLLQCYAFRALRFLFSMERNRPLFKRLFPTDLFEIFIDIGHYVRDISAYEELVSKLNLLVEDELKQIAENIESINQNKAPLKYIGNYAILDHLGSGAFGCVYKVRKHSGQNLLAMKEVNLHNPAFGKDKKDRDSSVRNIVSELTIIKEQLYHPNIVRYYKTFLENDRLYIVMELIEGAPLGEHFSSLKEKHHHFTEERLWKIFIQLCLALRYLHKEKRIVHRDLTPNNIMLGDKDKVTVTDFGLAKQKQENSKLTSVVGTILYSCPEVLKSEPYGEKADVWAVGCILYQMATLSPPFYSTNMLSLATKIVEAVYEPVPEGIYSEKVTDTISRCLTPDAEARPDIVEVSSMISDVMMKYLDNLSTSQLSLEKKLERERRRTQRYFMEANRNTVTCHHELAVLSHETFEKASLSSSSSGAASLKSELSESADLPPEGFQASYGKDEDRACDEILSDDNFNLENAEKDTYSEVDDELDISDNSSSSSSSPLKESTFNILKRSFSASGGERQSQTRDFTGGTGSRPRPGPQMGTFLWQASAGIAVSQRKVRQISDPIQQILIQLHKIIYITQLPPALHHNLKRRVIERFKKSLFSQQSNPCNLKSEIKKLSQGSPEPIEPNFFTADYHLLHRSSGGNSLSPNDPTGLPTSIELEEGITYEQMQTVIEEVLEESGYYNFTSNRYHSYPWGTKNHPTKR

Target ORF information:

RefSeq Version	XM_017005770.1
Organism	Homo sapiens(human)
Definition	Homo sapiens NIMA related kinase 10 (NEK10), transcript variant X14, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017005770.1

ORF Insert Sequence:

ATGCCTGATC AAGATAAAAA GGTGAAGACC ACAGAAAAAT CAACTGATAA ACAGCAAGAA 
ATCACCATCA GGGACTATTC AGATCTTAAA AGACTTCGGT GCCTTTTGAA CGTCCAATCA 
AGCAAACAAC AGCTTCCAGC CATTAACTTC GATAGTGCCC AAAATAGCAT GACGAAGTCT 
GAGCCCGCCA TCAGGGCGGG TGGACACAGA GCTCGGGGTC AGTGGCATGA ATCCACAGAA 
GCTGTTGAAC TTGAAAATTT TAGTATAAAC TACAAGAATG AGAGAAATTT CAGCAAACAT 
CCTCAGCGTA AACTATTTCA GGAGATCTTT ACCGCCTTGG TGAAAAATAG ACTCATAAGC 
AGAGAGTGGG TTAATCGAGC CCCATCTATT CATTTTCTGA GAGTGTTAAT CTGTCTGAGG 
CTACTAATGA GGGATCCATG TTATCAGGAA ATACTCCATA GCTTGGGTGG GATTGAAAAC 
CTAGCTCAGT ATATGGAGAT TGTAGCCAAT GAGTACCTCG GCTATGGAGA AGAGCAGCAC 
ACTGTGGACA AGCTGGTCAA CATGACATAT ATTTTTCAAA AACTTGCTGC AGTCAAAGAT 
CAAAGAGAAT GGGTCACCAC AAGTGGAGCC CACAAGACAT TAGTAAATTT ACTTGGTGCC 
CGAGATACTA ATGTTCTATT GGGTTCCCTT CTGGCTCTGG CTAGTTTAGC AGAAAGTCAA 
GAATGTAGGG AGAAGATAAG TGAACTCAAC ATTGTAGAAA ATCTGTTGAT GATTTTACAT 
GAATATGACT TGCTTTCTAA AAGACTAACA GCGGAGTTGC TGCGCCTACT TTGTGCAGAG 
CCCCAGGTGA AAGAGCAGGT GAAGCTCTAT GAGGGGATAC CGGTCCTCCT CAGTCTGCTC 
CACTCTGACC ACTTGAAGCT CCTCTGGAGC ATTGTCTGGA TTCTGGTACA GGTTTGTGAG 
GACCCTGAGA CCAGCGTGGA AATTCGCATT TGGGGAGGCA TCAAACAGCT TCTTCATATT 
TTACAAGGAG ACAGAAATTT TGTTTCTGAT CACTCCTCCA TTGGAAGCCT GTCCAGTGCA 
AATGCTGCAG GCCGAATCCA GCAGCTTCAT TTATCAGAAG ACTTGAGCCC TAGGGAAATA 
CAAGAAAATA CTTTCTCACT TCAAGCAGCC TGCTGTGCTG CCCTCACTGA GCTGGTGCTC 
AATGACACCA ATGCCCACCA GGTGGTTCAG GAAAATGGTG TATATACAAT AGCAAAATTA 
ATTTTACCAA ATAAGCAAAA GAATGCAGCA AAAAGTAATC TATTACAGTG TTATGCTTTC 
AGAGCCTTGA GATTTCTCTT CAGTATGGAA AGAAACAGAC CACTCTTTAA AAGACTTTTC 
CCCACAGACT TGTTTGAGAT CTTCATTGAC ATAGGGCATT ATGTACGTGA TATCAGTGCT 
TATGAAGAAT TGGTATCCAA GCTGAATTTA TTAGTGGAGG ATGAACTGAA GCAAATTGCT 
GAAAATATTG AAAGCATTAA TCAGAACAAA GCTCCTTTGA AATATATAGG CAACTATGCA 
ATTTTGGATC ATCTTGGAAG TGGAGCTTTT GGCTGTGTTT ACAAGGTTAG AAAGCATAGT 
GGTCAAAATC TTTTAGCAAT GAAAGAGGTC AATTTACATA ACCCAGCATT TGGAAAGGAT 
AAGAAAGATC GAGACAGCAG CGTAAGGAAT ATTGTTTCTG AATTAACAAT AATTAAAGAG 
CAGCTTTATC ATCCCAACAT TGTACGTTAT TACAAAACAT TTCTGGAAAA TGATAGGTTG 
TACATAGTTA TGGAGCTGAT AGAAGGAGCC CCGCTTGGAG AGCATTTCAG TTCTTTGAAG 
GAAAAACATC ACCATTTTAC TGAAGAAAGA CTATGGAAAA TATTTATACA GCTGTGCTTA 
GCTCTTCGAT ACTTACACAA GGAGAAGAGG ATTGTCCATA GAGATCTGAC ACCAAACAAC 
ATTATGTTGG GGGATAAGGA CAAAGTAACC GTTACTGACT TTGGCCTGGC AAAGCAAAAA 
CAAGAAAACA GTAAACTCAC CTCTGTGGTT GGAACAATCC TGTATTCTTG CCCCGAGGTA 
CTGAAGAGTG AGCCGTATGG GGAGAAGGCT GATGTCTGGG CAGTAGGCTG CATCCTTTAT 
CAGATGGCGA CTTTGAGTCC CCCCTTCTAC AGCACTAACA TGCTGTCCTT GGCTACAAAA 
ATAGTGGAGG CGGTATATGA ACCAGTCCCA GAAGGTATCT ACTCTGAAAA AGTAACAGAC 
ACCATCAGCA GGTGCCTCAC TCCTGATGCG GAAGCTCGTC CAGATATTGT AGAAGTCAGT 
TCGATGATAT CAGATGTCAT GATGAAATAT TTAGACAACT TATCTACATC CCAGTTGTCC 
TTGGAAAAGA AGCTAGAACG GGAACGAAGA CGCACACAAA GGTATTTTAT GGAAGCCAAC 
CGGAACACCG TCACATGTCA CCATGAGCTG GCTGTTCTAT CTCACGAGAC CTTTGAGAAG 
GCAAGTTTGA GTAGCAGCAG CAGTGGAGCA GCCAGCCTGA AAAGTGAACT TTCAGAAAGC 
GCAGACCTGC CCCCTGAAGG CTTCCAGGCC TCCTATGGTA AAGACGAAGA CAGGGCCTGT 
GACGAAATCC TGTCAGATGA TAACTTCAAC CTGGAAAATG CTGAGAAAGA TACATATTCA 
GAGGTAGATG ATGAATTGGA CATTTCGGAT AACTCCAGCA GCTCCAGTTC AAGCCCTCTG 
AAAGAATCTA CATTCAACAT TTTAAAGAGA AGTTTTAGTG CTTCAGGAGG AGAAAGACAA 
TCCCAAACAA GGGACTTCAC TGGAGGAACA GGATCAAGAC CAAGACCAGG GCCACAGATG 
GGCACATTCT TGTGGCAAGC ATCAGCAGGA ATTGCTGTGT CCCAGAGGAA AGTGCGTCAG 
ATCAGTGATC CTATTCAGCA GATATTAATT CAGCTGCACA AAATAATCTA TATCACACAG 
CTTCCTCCAG CTTTGCACCA CAATTTGAAA AGAAGGGTTA TAGAGAGATT CAAGAAATCC 
CTCTTCAGCC AGCAGAGTAA CCCTTGTAAT TTGAAATCTG AAATTAAAAA GTTATCTCAG 
GGATCTCCAG AACCGATTGA GCCCAACTTT TTCACAGCAG ATTACCATTT ATTACATCGT 
TCATCCGGTG GAAACAGCCT GTCCCCAAAT GACCCTACAG GTTTACCAAC CAGCATTGAA 
TTGGAGGAAG GAATAACATA TGAACAGATG CAGACTGTGA TTGAAGAAGT CCTTGAGGAA 
AGTGGCTATT ACAATTTTAC ATCTAACAGG TATCATTCCT ATCCATGGGG GACCAAGAAT 
CACCCAACCA AAAGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NEK10 ( XM_017005770.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_016861259.1 Homo sapiens NIMA related kinase 10 (NEK10), transcript variant X15, mRNA.

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CloneID	OHu93837
Clone ID Related Accession (Same CDS sequence)	XM_017005769.1 , XM_017005770.1 , XM_017005770.2
Accession Version	XM_017005770.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3378bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	serine/threonine-protein kinase Nek10 isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000003.12) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_017005770.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCTGATC AAGATAAAAA GGTGAAGACC ACAGAAAAAT CAACTGATAA ACAGCAAGAA 
ATCACCATCA GGGACTATTC AGATCTTAAA AGACTTCGGT GCCTTTTGAA CGTCCAATCA 
AGCAAACAAC AGCTTCCAGC CATTAACTTC GATAGTGCCC AAAATAGCAT GACGAAGTCT 
GAGCCCGCCA TCAGGGCGGG TGGACACAGA GCTCGGGGTC AGTGGCATGA ATCCACAGAA 
GCTGTTGAAC TTGAAAATTT TAGTATAAAC TACAAGAATG AGAGAAATTT CAGCAAACAT 
CCTCAGCGTA AACTATTTCA GGAGATCTTT ACCGCCTTGG TGAAAAATAG ACTCATAAGC 
AGAGAGTGGG TTAATCGAGC CCCATCTATT CATTTTCTGA GAGTGTTAAT CTGTCTGAGG 
CTACTAATGA GGGATCCATG TTATCAGGAA ATACTCCATA GCTTGGGTGG GATTGAAAAC 
CTAGCTCAGT ATATGGAGAT TGTAGCCAAT GAGTACCTCG GCTATGGAGA AGAGCAGCAC 
ACTGTGGACA AGCTGGTCAA CATGACATAT ATTTTTCAAA AACTTGCTGC AGTCAAAGAT 
CAAAGAGAAT GGGTCACCAC AAGTGGAGCC CACAAGACAT TAGTAAATTT ACTTGGTGCC 
CGAGATACTA ATGTTCTATT GGGTTCCCTT CTGGCTCTGG CTAGTTTAGC AGAAAGTCAA 
GAATGTAGGG AGAAGATAAG TGAACTCAAC ATTGTAGAAA ATCTGTTGAT GATTTTACAT 
GAATATGACT TGCTTTCTAA AAGACTAACA GCGGAGTTGC TGCGCCTACT TTGTGCAGAG 
CCCCAGGTGA AAGAGCAGGT GAAGCTCTAT GAGGGGATAC CGGTCCTCCT CAGTCTGCTC 
CACTCTGACC ACTTGAAGCT CCTCTGGAGC ATTGTCTGGA TTCTGGTACA GGTTTGTGAG 
GACCCTGAGA CCAGCGTGGA AATTCGCATT TGGGGAGGCA TCAAACAGCT TCTTCATATT 
TTACAAGGAG ACAGAAATTT TGTTTCTGAT CACTCCTCCA TTGGAAGCCT GTCCAGTGCA 
AATGCTGCAG GCCGAATCCA GCAGCTTCAT TTATCAGAAG ACTTGAGCCC TAGGGAAATA 
CAAGAAAATA CTTTCTCACT TCAAGCAGCC TGCTGTGCTG CCCTCACTGA GCTGGTGCTC 
AATGACACCA ATGCCCACCA GGTGGTTCAG GAAAATGGTG TATATACAAT AGCAAAATTA 
ATTTTACCAA ATAAGCAAAA GAATGCAGCA AAAAGTAATC TATTACAGTG TTATGCTTTC 
AGAGCCTTGA GATTTCTCTT CAGTATGGAA AGAAACAGAC CACTCTTTAA AAGACTTTTC 
CCCACAGACT TGTTTGAGAT CTTCATTGAC ATAGGGCATT ATGTACGTGA TATCAGTGCT 
TATGAAGAAT TGGTATCCAA GCTGAATTTA TTAGTGGAGG ATGAACTGAA GCAAATTGCT 
GAAAATATTG AAAGCATTAA TCAGAACAAA GCTCCTTTGA AATATATAGG CAACTATGCA 
ATTTTGGATC ATCTTGGAAG TGGAGCTTTT GGCTGTGTTT ACAAGGTTAG AAAGCATAGT 
GGTCAAAATC TTTTAGCAAT GAAAGAGGTC AATTTACATA ACCCAGCATT TGGAAAGGAT 
AAGAAAGATC GAGACAGCAG CGTAAGGAAT ATTGTTTCTG AATTAACAAT AATTAAAGAG 
CAGCTTTATC ATCCCAACAT TGTACGTTAT TACAAAACAT TTCTGGAAAA TGATAGGTTG 
TACATAGTTA TGGAGCTGAT AGAAGGAGCC CCGCTTGGAG AGCATTTCAG TTCTTTGAAG 
GAAAAACATC ACCATTTTAC TGAAGAAAGA CTATGGAAAA TATTTATACA GCTGTGCTTA 
GCTCTTCGAT ACTTACACAA GGAGAAGAGG ATTGTCCATA GAGATCTGAC ACCAAACAAC 
ATTATGTTGG GGGATAAGGA CAAAGTAACC GTTACTGACT TTGGCCTGGC AAAGCAAAAA 
CAAGAAAACA GTAAACTCAC CTCTGTGGTT GGAACAATCC TGTATTCTTG CCCCGAGGTA 
CTGAAGAGTG AGCCGTATGG GGAGAAGGCT GATGTCTGGG CAGTAGGCTG CATCCTTTAT 
CAGATGGCGA CTTTGAGTCC CCCCTTCTAC AGCACTAACA TGCTGTCCTT GGCTACAAAA 
ATAGTGGAGG CGGTATATGA ACCAGTCCCA GAAGGTATCT ACTCTGAAAA AGTAACAGAC 
ACCATCAGCA GGTGCCTCAC TCCTGATGCG GAAGCTCGTC CAGATATTGT AGAAGTCAGT 
TCGATGATAT CAGATGTCAT GATGAAATAT TTAGACAACT TATCTACATC CCAGTTGTCC 
TTGGAAAAGA AGCTAGAACG GGAACGAAGA CGCACACAAA GGTATTTTAT GGAAGCCAAC 
CGGAACACCG TCACATGTCA CCATGAGCTG GCTGTTCTAT CTCACGAGAC CTTTGAGAAG 
GCAAGTTTGA GTAGCAGCAG CAGTGGAGCA GCCAGCCTGA AAAGTGAACT TTCAGAAAGC 
GCAGACCTGC CCCCTGAAGG CTTCCAGGCC TCCTATGGTA AAGACGAAGA CAGGGCCTGT 
GACGAAATCC TGTCAGATGA TAACTTCAAC CTGGAAAATG CTGAGAAAGA TACATATTCA 
GAGGTAGATG ATGAATTGGA CATTTCGGAT AACTCCAGCA GCTCCAGTTC AAGCCCTCTG 
AAAGAATCTA CATTCAACAT TTTAAAGAGA AGTTTTAGTG CTTCAGGAGG AGAAAGACAA 
TCCCAAACAA GGGACTTCAC TGGAGGAACA GGATCAAGAC CAAGACCAGG GCCACAGATG 
GGCACATTCT TGTGGCAAGC ATCAGCAGGA ATTGCTGTGT CCCAGAGGAA AGTGCGTCAG 
ATCAGTGATC CTATTCAGCA GATATTAATT CAGCTGCACA AAATAATCTA TATCACACAG 
CTTCCTCCAG CTTTGCACCA CAATTTGAAA AGAAGGGTTA TAGAGAGATT CAAGAAATCC 
CTCTTCAGCC AGCAGAGTAA CCCTTGTAAT TTGAAATCTG AAATTAAAAA GTTATCTCAG 
GGATCTCCAG AACCGATTGA GCCCAACTTT TTCACAGCAG ATTACCATTT ATTACATCGT 
TCATCCGGTG GAAACAGCCT GTCCCCAAAT GACCCTACAG GTTTACCAAC CAGCATTGAA 
TTGGAGGAAG GAATAACATA TGAACAGATG CAGACTGTGA TTGAAGAAGT CCTTGAGGAA 
AGTGGCTATT ACAATTTTAC ATCTAACAGG TATCATTCCT ATCCATGGGG GACCAAGAAT 
CACCCAACCA AAAGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016861259.1
CDS	274..3651
Translation	MPDQDKKVKTTEKSTDKQQEITIRDYSDLKRLRCLLNVQSSKQQLPAINFDSAQNSMTKSEPAIRAGGHRARGQWHESTEAVELENFSINYKNERNFSKHPQRKLFQEIFTALVKNRLISREWVNRAPSIHFLRVLICLRLLMRDPCYQEILHSLGGIENLAQYMEIVANEYLGYGEEQHTVDKLVNMTYIFQKLAAVKDQREWVTTSGAHKTLVNLLGARDTNVLLGSLLALASLAESQECREKISELNIVENLLMILHEYDLLSKRLTAELLRLLCAEPQVKEQVKLYEGIPVLLSLLHSDHLKLLWSIVWILVQVCEDPETSVEIRIWGGIKQLLHILQGDRNFVSDHSSIGSLSSANAAGRIQQLHLSEDLSPREIQENTFSLQAACCAALTELVLNDTNAHQVVQENGVYTIAKLILPNKQKNAAKSNLLQCYAFRALRFLFSMERNRPLFKRLFPTDLFEIFIDIGHYVRDISAYEELVSKLNLLVEDELKQIAENIESINQNKAPLKYIGNYAILDHLGSGAFGCVYKVRKHSGQNLLAMKEVNLHNPAFGKDKKDRDSSVRNIVSELTIIKEQLYHPNIVRYYKTFLENDRLYIVMELIEGAPLGEHFSSLKEKHHHFTEERLWKIFIQLCLALRYLHKEKRIVHRDLTPNNIMLGDKDKVTVTDFGLAKQKQENSKLTSVVGTILYSCPEVLKSEPYGEKADVWAVGCILYQMATLSPPFYSTNMLSLATKIVEAVYEPVPEGIYSEKVTDTISRCLTPDAEARPDIVEVSSMISDVMMKYLDNLSTSQLSLEKKLERERRRTQRYFMEANRNTVTCHHELAVLSHETFEKASLSSSSSGAASLKSELSESADLPPEGFQASYGKDEDRACDEILSDDNFNLENAEKDTYSEVDDELDISDNSSSSSSSPLKESTFNILKRSFSASGGERQSQTRDFTGGTGSRPRPGPQMGTFLWQASAGIAVSQRKVRQISDPIQQILIQLHKIIYITQLPPALHHNLKRRVIERFKKSLFSQQSNPCNLKSEIKKLSQGSPEPIEPNFFTADYHLLHRSSGGNSLSPNDPTGLPTSIELEEGITYEQMQTVIEEVLEESGYYNFTSNRYHSYPWGTKNHPTKR

Target ORF information:

RefSeq Version	XM_017005770.2
Organism	Homo sapiens(human)
Definition	Homo sapiens NIMA related kinase 10 (NEK10), transcript variant X15, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017005770.2

ORF Insert Sequence:

ATGCCTGATC AAGATAAAAA GGTGAAGACC ACAGAAAAAT CAACTGATAA ACAGCAAGAA 
ATCACCATCA GGGACTATTC AGATCTTAAA AGACTTCGGT GCCTTTTGAA CGTCCAATCA 
AGCAAACAAC AGCTTCCAGC CATTAACTTC GATAGTGCCC AAAATAGCAT GACGAAGTCT 
GAGCCCGCCA TCAGGGCGGG TGGACACAGA GCTCGGGGTC AGTGGCATGA ATCCACAGAA 
GCTGTTGAAC TTGAAAATTT TAGTATAAAC TACAAGAATG AGAGAAATTT CAGCAAACAT 
CCTCAGCGTA AACTATTTCA GGAGATCTTT ACCGCCTTGG TGAAAAATAG ACTCATAAGC 
AGAGAGTGGG TTAATCGAGC CCCATCTATT CATTTTCTGA GAGTGTTAAT CTGTCTGAGG 
CTACTAATGA GGGATCCATG TTATCAGGAA ATACTCCATA GCTTGGGTGG GATTGAAAAC 
CTAGCTCAGT ATATGGAGAT TGTAGCCAAT GAGTACCTCG GCTATGGAGA AGAGCAGCAC 
ACTGTGGACA AGCTGGTCAA CATGACATAT ATTTTTCAAA AACTTGCTGC AGTCAAAGAT 
CAAAGAGAAT GGGTCACCAC AAGTGGAGCC CACAAGACAT TAGTAAATTT ACTTGGTGCC 
CGAGATACTA ATGTTCTATT GGGTTCCCTT CTGGCTCTGG CTAGTTTAGC AGAAAGTCAA 
GAATGTAGGG AGAAGATAAG TGAACTCAAC ATTGTAGAAA ATCTGTTGAT GATTTTACAT 
GAATATGACT TGCTTTCTAA AAGACTAACA GCGGAGTTGC TGCGCCTACT TTGTGCAGAG 
CCCCAGGTGA AAGAGCAGGT GAAGCTCTAT GAGGGGATAC CGGTCCTCCT CAGTCTGCTC 
CACTCTGACC ACTTGAAGCT CCTCTGGAGC ATTGTCTGGA TTCTGGTACA GGTTTGTGAG 
GACCCTGAGA CCAGCGTGGA AATTCGCATT TGGGGAGGCA TCAAACAGCT TCTTCATATT 
TTACAAGGAG ACAGAAATTT TGTTTCTGAT CACTCCTCCA TTGGAAGCCT GTCCAGTGCA 
AATGCTGCAG GCCGAATCCA GCAGCTTCAT TTATCAGAAG ACTTGAGCCC TAGGGAAATA 
CAAGAAAATA CTTTCTCACT TCAAGCAGCC TGCTGTGCTG CCCTCACTGA GCTGGTGCTC 
AATGACACCA ATGCCCACCA GGTGGTTCAG GAAAATGGTG TATATACAAT AGCAAAATTA 
ATTTTACCAA ATAAGCAAAA GAATGCAGCA AAAAGTAATC TATTACAGTG TTATGCTTTC 
AGAGCCTTGA GATTTCTCTT CAGTATGGAA AGAAACAGAC CACTCTTTAA AAGACTTTTC 
CCCACAGACT TGTTTGAGAT CTTCATTGAC ATAGGGCATT ATGTACGTGA TATCAGTGCT 
TATGAAGAAT TGGTATCCAA GCTGAATTTA TTAGTGGAGG ATGAACTGAA GCAAATTGCT 
GAAAATATTG AAAGCATTAA TCAGAACAAA GCTCCTTTGA AATATATAGG CAACTATGCA 
ATTTTGGATC ATCTTGGAAG TGGAGCTTTT GGCTGTGTTT ACAAGGTTAG AAAGCATAGT 
GGTCAAAATC TTTTAGCAAT GAAAGAGGTC AATTTACATA ACCCAGCATT TGGAAAGGAT 
AAGAAAGATC GAGACAGCAG CGTAAGGAAT ATTGTTTCTG AATTAACAAT AATTAAAGAG 
CAGCTTTATC ATCCCAACAT TGTACGTTAT TACAAAACAT TTCTGGAAAA TGATAGGTTG 
TACATAGTTA TGGAGCTGAT AGAAGGAGCC CCGCTTGGAG AGCATTTCAG TTCTTTGAAG 
GAAAAACATC ACCATTTTAC TGAAGAAAGA CTATGGAAAA TATTTATACA GCTGTGCTTA 
GCTCTTCGAT ACTTACACAA GGAGAAGAGG ATTGTCCATA GAGATCTGAC ACCAAACAAC 
ATTATGTTGG GGGATAAGGA CAAAGTAACC GTTACTGACT TTGGCCTGGC AAAGCAAAAA 
CAAGAAAACA GTAAACTCAC CTCTGTGGTT GGAACAATCC TGTATTCTTG CCCCGAGGTA 
CTGAAGAGTG AGCCGTATGG GGAGAAGGCT GATGTCTGGG CAGTAGGCTG CATCCTTTAT 
CAGATGGCGA CTTTGAGTCC CCCCTTCTAC AGCACTAACA TGCTGTCCTT GGCTACAAAA 
ATAGTGGAGG CGGTATATGA ACCAGTCCCA GAAGGTATCT ACTCTGAAAA AGTAACAGAC 
ACCATCAGCA GGTGCCTCAC TCCTGATGCG GAAGCTCGTC CAGATATTGT AGAAGTCAGT 
TCGATGATAT CAGATGTCAT GATGAAATAT TTAGACAACT TATCTACATC CCAGTTGTCC 
TTGGAAAAGA AGCTAGAACG GGAACGAAGA CGCACACAAA GGTATTTTAT GGAAGCCAAC 
CGGAACACCG TCACATGTCA CCATGAGCTG GCTGTTCTAT CTCACGAGAC CTTTGAGAAG 
GCAAGTTTGA GTAGCAGCAG CAGTGGAGCA GCCAGCCTGA AAAGTGAACT TTCAGAAAGC 
GCAGACCTGC CCCCTGAAGG CTTCCAGGCC TCCTATGGTA AAGACGAAGA CAGGGCCTGT 
GACGAAATCC TGTCAGATGA TAACTTCAAC CTGGAAAATG CTGAGAAAGA TACATATTCA 
GAGGTAGATG ATGAATTGGA CATTTCGGAT AACTCCAGCA GCTCCAGTTC AAGCCCTCTG 
AAAGAATCTA CATTCAACAT TTTAAAGAGA AGTTTTAGTG CTTCAGGAGG AGAAAGACAA 
TCCCAAACAA GGGACTTCAC TGGAGGAACA GGATCAAGAC CAAGACCAGG GCCACAGATG 
GGCACATTCT TGTGGCAAGC ATCAGCAGGA ATTGCTGTGT CCCAGAGGAA AGTGCGTCAG 
ATCAGTGATC CTATTCAGCA GATATTAATT CAGCTGCACA AAATAATCTA TATCACACAG 
CTTCCTCCAG CTTTGCACCA CAATTTGAAA AGAAGGGTTA TAGAGAGATT CAAGAAATCC 
CTCTTCAGCC AGCAGAGTAA CCCTTGTAAT TTGAAATCTG AAATTAAAAA GTTATCTCAG 
GGATCTCCAG AACCGATTGA GCCCAACTTT TTCACAGCAG ATTACCATTT ATTACATCGT 
TCATCCGGTG GAAACAGCCT GTCCCCAAAT GACCCTACAG GTTTACCAAC CAGCATTGAA 
TTGGAGGAAG GAATAACATA TGAACAGATG CAGACTGTGA TTGAAGAAGT CCTTGAGGAA 
AGTGGCTATT ACAATTTTAC ATCTAACAGG TATCATTCCT ATCCATGGGG GACCAAGAAT 
CACCCAACCA AAAGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NEK10 ( XM_017005772.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_016861261.1 Homo sapiens NIMA related kinase 10 (NEK10), transcript variant X18, mRNA.

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CloneID	OHu93838
Clone ID Related Accession (Same CDS sequence)	XM_017005772.2 , XM_017005772.1
Accession Version	XM_017005772.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3291bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	serine/threonine-protein kinase Nek10 isoform X9
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000003.12) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_017005772.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCTGATC AAGATAAAAA GGTGAAGACC ACAGAAAAAT CAACTGATAA ACAGCAAGAA 
ATCACCATCA GGGACTATTC AGATCTTAAA AGACTTCGGT GCCTTTTGAA CGTCCAATCA 
AGCAAACAAC AGCTTCCAGC CATTAACTTC GATAGTGCCC AAAATAGCAT GACGAAGTCT 
GAGCCCGCCA TCAGGGCGGG TGGACACAGA GCTCGGGGTC AGTGGCATGA ATCCACAGAA 
GCTGTTGAAC TTGAAAATTT TAGTATAAAC TACAAGAATG AGAGAAATTT CAGCAAACAT 
CCTCAGCGTA AACTATTTCA GGAGATCTTT ACCGCCTTGG TGAAAAATAG ACTCATAAGC 
AGAGAGTGGG TTAATCGAGC CCCATCTATT CATTTTCTGA GAGTGTTAAT CTGTCTGAGG 
CTACTAATGA GGGATCCATG TTATCAGGAA ATACTCCATA GCTTGGGTGG GATTGAAAAC 
CTAGCTCAGT ATATGGAGAT TGTAGCCAAT GAGTACCTCG GCTATGGAGA AGAGCAGCAC 
ACTGTGGACA AGCTGGTCAA CATGACATAT ATTTTTCAAA AACTTGCTGC AGTCAAAGAT 
CAAAGAGAAT GGGTCACCAC AAGTGGAGCC CACAAGACAT TAGTAAATTT ACTTGGTGCC 
CGAGATACTA ATGTTCTATT GGGTTCCCTT CTGGCTCTGG CTAGTTTAGC AGAAAGACTA 
ACAGCGGAGT TGCTGCGCCT ACTTTGTGCA GAGCCCCAGG TGAAAGAGCA GGTGAAGCTC 
TATGAGGGGA TACCGGTCCT CCTCAGTCTG CTCCACTCTG ACCACTTGAA GCTCCTCTGG 
AGCATTGTCT GGATTCTGGT ACAGGTTTGT GAGGACCCTG AGACCAGCGT GGAAATTCGC 
ATTTGGGGAG GCATCAAACA GCTTCTTCAT ATTTTACAAG GAGACAGAAA TTTTGTTTCT 
GATCACTCCT CCATTGGAAG CCTGTCCAGT GCAAATGCTG CAGGCCGAAT CCAGCAGCTT 
CATTTATCAG AAGACTTGAG CCCTAGGGAA ATACAAGAAA ATACTTTCTC ACTTCAAGCA 
GCCTGCTGTG CTGCCCTCAC TGAGCTGGTG CTCAATGACA CCAATGCCCA CCAGGTGGTT 
CAGGAAAATG GTGTATATAC AATAGCAAAA TTAATTTTAC CAAATAAGCA AAAGAATGCA 
GCAAAAAGTA ATCTATTACA GTGTTATGCT TTCAGAGCCT TGAGATTTCT CTTCAGTATG 
GAAAGAAACA GACCACTCTT TAAAAGACTT TTCCCCACAG ACTTGTTTGA GATCTTCATT 
GACATAGGGC ATTATGTACG TGATATCAGT GCTTATGAAG AATTGGTATC CAAGCTGAAT 
TTATTAGTGG AGGATGAACT GAAGCAAATT GCTGAAAATA TTGAAAGCAT TAATCAGAAC 
AAAGCTCCTT TGAAATATAT AGGCAACTAT GCAATTTTGG ATCATCTTGG AAGTGGAGCT 
TTTGGCTGTG TTTACAAGGT TAGAAAGCAT AGTGGTCAAA ATCTTTTAGC AATGAAAGAG 
GTCAATTTAC ATAACCCAGC ATTTGGAAAG GATAAGAAAG ATCGAGACAG CAGCGTAAGG 
AATATTGTTT CTGAATTAAC AATAATTAAA GAGCAGCTTT ATCATCCCAA CATTGTACGT 
TATTACAAAA CATTTCTGGA AAATGATAGG TTGTACATAG TTATGGAGCT GATAGAAGGA 
GCCCCGCTTG GAGAGCATTT CAGTTCTTTG AAGGAAAAAC ATCACCATTT TACTGAAGAA 
AGACTATGGA AAATATTTAT ACAGCTGTGC TTAGCTCTTC GATACTTACA CAAGGAGAAG 
AGGATTGTCC ATAGAGATCT GACACCAAAC AACATTATGT TGGGGGATAA GGACAAAGTA 
ACCGTTACTG ACTTTGGCCT GGCAAAGCAA AAACAAGAAA ACAGTAAACT CACCTCTGTG 
GTTGGAACAA TCCTGTATTC TTGCCCCGAG GTACTGAAGA GTGAGCCGTA TGGGGAGAAG 
GCTGATGTCT GGGCAGTAGG CTGCATCCTT TATCAGATGG CGACTTTGAG TCCCCCCTTC 
TACAGCACTA ACATGCTGTC CTTGGCTACA AAAATAGTGG AGGCGGTATA TGAACCAGTC 
CCAGAAGGTA TCTACTCTGA AAAAGTAACA GACACCATCA GCAGGTGCCT CACTCCTGAT 
GCGGAAGCTC GTCCAGATAT TGTAGAAGTC AGTTCGATGA TATCAGATGT CATGATGAAA 
TATTTAGACA ACTTATCTAC ATCCCAGTTG TCCTTGGAAA AGAAGCTAGA ACGGGAACGA 
AGACGCACAC AAAGGTATTT TATGGAAGCC AACCGGAACA CCGTCACATG TCACCATGAG 
CTGGCTGTTC TATCTCACGA GACCTTTGAG AAGGCAAGTT TGAGTAGCAG CAGCAGTGGA 
GCAGCCAGCC TGAAAAGTGA ACTTTCAGAA AGCGCAGACC TGCCCCCTGA AGGCTTCCAG 
GCCTCCTATG GTAAAGACGA AGACAGGGCC TGTGACGAAA TCCTGTCAGA TGATAACTTC 
AACCTGGAAA ATGCTGAGAA AGATACATAT TCAGAGGTAG ATGATGAATT GGACATTTCG 
GATAACTCCA GCAGCTCCAG TTCAAGCCCT CTGAAAGAAT CTACATTCAA CATTTTAAAG 
AGAAGTTTTA GTGCTTCAGG AGGAGAAAGA CAATCCCAAA CAAGGGACTT CACTGGAGGA 
ACAGGATCAA GACCAAGACC AGGGCCACAG ATGGGCACAT TCTTGTGGCA AGCATCAGCA 
GGAATTGCTG TGTCCCAGAG GAAAGTGCGT CAGATCAGTG ATCCTATTCA GCAGATATTA 
ATTCAGCTGC ACAAAATAAT CTATATCACA CAGCTTCCTC CAGCTTTGCA CCACAATTTG 
AAAAGAAGGG TTATAGAGAG ATTCAAGAAA TCCCTCTTCA GCCAGCAGAG TAACCCTTGT 
AATTTGAAAT CTGAAATTAA AAAGTTATCT CAGGGATCTC CAGAACCGAT TGAGCCCAAC 
TTTTTCACAG CAGATTACCA TTTATTACAT CGTTCATCCG GTGGAAACAG CCTGTCCCCA 
AATGACCCTA CAGGTTTACC AACCAGCATT GAATTGGAGG AAGGAATAAC ATATGAACAG 
ATGCAGACTG TGATTGAAGA AGTCCTTGAG GAAAGTGGCT ATTACAATTT TACATCTAAC 
AGGTATCATT CCTATCCATG GGGGACCAAG AATCACCCAA CCAAAAGATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016861261.1
CDS	274..3564
Translation	MPDQDKKVKTTEKSTDKQQEITIRDYSDLKRLRCLLNVQSSKQQLPAINFDSAQNSMTKSEPAIRAGGHRARGQWHESTEAVELENFSINYKNERNFSKHPQRKLFQEIFTALVKNRLISREWVNRAPSIHFLRVLICLRLLMRDPCYQEILHSLGGIENLAQYMEIVANEYLGYGEEQHTVDKLVNMTYIFQKLAAVKDQREWVTTSGAHKTLVNLLGARDTNVLLGSLLALASLAERLTAELLRLLCAEPQVKEQVKLYEGIPVLLSLLHSDHLKLLWSIVWILVQVCEDPETSVEIRIWGGIKQLLHILQGDRNFVSDHSSIGSLSSANAAGRIQQLHLSEDLSPREIQENTFSLQAACCAALTELVLNDTNAHQVVQENGVYTIAKLILPNKQKNAAKSNLLQCYAFRALRFLFSMERNRPLFKRLFPTDLFEIFIDIGHYVRDISAYEELVSKLNLLVEDELKQIAENIESINQNKAPLKYIGNYAILDHLGSGAFGCVYKVRKHSGQNLLAMKEVNLHNPAFGKDKKDRDSSVRNIVSELTIIKEQLYHPNIVRYYKTFLENDRLYIVMELIEGAPLGEHFSSLKEKHHHFTEERLWKIFIQLCLALRYLHKEKRIVHRDLTPNNIMLGDKDKVTVTDFGLAKQKQENSKLTSVVGTILYSCPEVLKSEPYGEKADVWAVGCILYQMATLSPPFYSTNMLSLATKIVEAVYEPVPEGIYSEKVTDTISRCLTPDAEARPDIVEVSSMISDVMMKYLDNLSTSQLSLEKKLERERRRTQRYFMEANRNTVTCHHELAVLSHETFEKASLSSSSSGAASLKSELSESADLPPEGFQASYGKDEDRACDEILSDDNFNLENAEKDTYSEVDDELDISDNSSSSSSSPLKESTFNILKRSFSASGGERQSQTRDFTGGTGSRPRPGPQMGTFLWQASAGIAVSQRKVRQISDPIQQILIQLHKIIYITQLPPALHHNLKRRVIERFKKSLFSQQSNPCNLKSEIKKLSQGSPEPIEPNFFTADYHLLHRSSGGNSLSPNDPTGLPTSIELEEGITYEQMQTVIEEVLEESGYYNFTSNRYHSYPWGTKNHPTKR

Target ORF information:

RefSeq Version	XM_017005772.2
Organism	Homo sapiens(human)
Definition	Homo sapiens NIMA related kinase 10 (NEK10), transcript variant X18, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017005772.2

ORF Insert Sequence:

ATGCCTGATC AAGATAAAAA GGTGAAGACC ACAGAAAAAT CAACTGATAA ACAGCAAGAA 
ATCACCATCA GGGACTATTC AGATCTTAAA AGACTTCGGT GCCTTTTGAA CGTCCAATCA 
AGCAAACAAC AGCTTCCAGC CATTAACTTC GATAGTGCCC AAAATAGCAT GACGAAGTCT 
GAGCCCGCCA TCAGGGCGGG TGGACACAGA GCTCGGGGTC AGTGGCATGA ATCCACAGAA 
GCTGTTGAAC TTGAAAATTT TAGTATAAAC TACAAGAATG AGAGAAATTT CAGCAAACAT 
CCTCAGCGTA AACTATTTCA GGAGATCTTT ACCGCCTTGG TGAAAAATAG ACTCATAAGC 
AGAGAGTGGG TTAATCGAGC CCCATCTATT CATTTTCTGA GAGTGTTAAT CTGTCTGAGG 
CTACTAATGA GGGATCCATG TTATCAGGAA ATACTCCATA GCTTGGGTGG GATTGAAAAC 
CTAGCTCAGT ATATGGAGAT TGTAGCCAAT GAGTACCTCG GCTATGGAGA AGAGCAGCAC 
ACTGTGGACA AGCTGGTCAA CATGACATAT ATTTTTCAAA AACTTGCTGC AGTCAAAGAT 
CAAAGAGAAT GGGTCACCAC AAGTGGAGCC CACAAGACAT TAGTAAATTT ACTTGGTGCC 
CGAGATACTA ATGTTCTATT GGGTTCCCTT CTGGCTCTGG CTAGTTTAGC AGAAAGACTA 
ACAGCGGAGT TGCTGCGCCT ACTTTGTGCA GAGCCCCAGG TGAAAGAGCA GGTGAAGCTC 
TATGAGGGGA TACCGGTCCT CCTCAGTCTG CTCCACTCTG ACCACTTGAA GCTCCTCTGG 
AGCATTGTCT GGATTCTGGT ACAGGTTTGT GAGGACCCTG AGACCAGCGT GGAAATTCGC 
ATTTGGGGAG GCATCAAACA GCTTCTTCAT ATTTTACAAG GAGACAGAAA TTTTGTTTCT 
GATCACTCCT CCATTGGAAG CCTGTCCAGT GCAAATGCTG CAGGCCGAAT CCAGCAGCTT 
CATTTATCAG AAGACTTGAG CCCTAGGGAA ATACAAGAAA ATACTTTCTC ACTTCAAGCA 
GCCTGCTGTG CTGCCCTCAC TGAGCTGGTG CTCAATGACA CCAATGCCCA CCAGGTGGTT 
CAGGAAAATG GTGTATATAC AATAGCAAAA TTAATTTTAC CAAATAAGCA AAAGAATGCA 
GCAAAAAGTA ATCTATTACA GTGTTATGCT TTCAGAGCCT TGAGATTTCT CTTCAGTATG 
GAAAGAAACA GACCACTCTT TAAAAGACTT TTCCCCACAG ACTTGTTTGA GATCTTCATT 
GACATAGGGC ATTATGTACG TGATATCAGT GCTTATGAAG AATTGGTATC CAAGCTGAAT 
TTATTAGTGG AGGATGAACT GAAGCAAATT GCTGAAAATA TTGAAAGCAT TAATCAGAAC 
AAAGCTCCTT TGAAATATAT AGGCAACTAT GCAATTTTGG ATCATCTTGG AAGTGGAGCT 
TTTGGCTGTG TTTACAAGGT TAGAAAGCAT AGTGGTCAAA ATCTTTTAGC AATGAAAGAG 
GTCAATTTAC ATAACCCAGC ATTTGGAAAG GATAAGAAAG ATCGAGACAG CAGCGTAAGG 
AATATTGTTT CTGAATTAAC AATAATTAAA GAGCAGCTTT ATCATCCCAA CATTGTACGT 
TATTACAAAA CATTTCTGGA AAATGATAGG TTGTACATAG TTATGGAGCT GATAGAAGGA 
GCCCCGCTTG GAGAGCATTT CAGTTCTTTG AAGGAAAAAC ATCACCATTT TACTGAAGAA 
AGACTATGGA AAATATTTAT ACAGCTGTGC TTAGCTCTTC GATACTTACA CAAGGAGAAG 
AGGATTGTCC ATAGAGATCT GACACCAAAC AACATTATGT TGGGGGATAA GGACAAAGTA 
ACCGTTACTG ACTTTGGCCT GGCAAAGCAA AAACAAGAAA ACAGTAAACT CACCTCTGTG 
GTTGGAACAA TCCTGTATTC TTGCCCCGAG GTACTGAAGA GTGAGCCGTA TGGGGAGAAG 
GCTGATGTCT GGGCAGTAGG CTGCATCCTT TATCAGATGG CGACTTTGAG TCCCCCCTTC 
TACAGCACTA ACATGCTGTC CTTGGCTACA AAAATAGTGG AGGCGGTATA TGAACCAGTC 
CCAGAAGGTA TCTACTCTGA AAAAGTAACA GACACCATCA GCAGGTGCCT CACTCCTGAT 
GCGGAAGCTC GTCCAGATAT TGTAGAAGTC AGTTCGATGA TATCAGATGT CATGATGAAA 
TATTTAGACA ACTTATCTAC ATCCCAGTTG TCCTTGGAAA AGAAGCTAGA ACGGGAACGA 
AGACGCACAC AAAGGTATTT TATGGAAGCC AACCGGAACA CCGTCACATG TCACCATGAG 
CTGGCTGTTC TATCTCACGA GACCTTTGAG AAGGCAAGTT TGAGTAGCAG CAGCAGTGGA 
GCAGCCAGCC TGAAAAGTGA ACTTTCAGAA AGCGCAGACC TGCCCCCTGA AGGCTTCCAG 
GCCTCCTATG GTAAAGACGA AGACAGGGCC TGTGACGAAA TCCTGTCAGA TGATAACTTC 
AACCTGGAAA ATGCTGAGAA AGATACATAT TCAGAGGTAG ATGATGAATT GGACATTTCG 
GATAACTCCA GCAGCTCCAG TTCAAGCCCT CTGAAAGAAT CTACATTCAA CATTTTAAAG 
AGAAGTTTTA GTGCTTCAGG AGGAGAAAGA CAATCCCAAA CAAGGGACTT CACTGGAGGA 
ACAGGATCAA GACCAAGACC AGGGCCACAG ATGGGCACAT TCTTGTGGCA AGCATCAGCA 
GGAATTGCTG TGTCCCAGAG GAAAGTGCGT CAGATCAGTG ATCCTATTCA GCAGATATTA 
ATTCAGCTGC ACAAAATAAT CTATATCACA CAGCTTCCTC CAGCTTTGCA CCACAATTTG 
AAAAGAAGGG TTATAGAGAG ATTCAAGAAA TCCCTCTTCA GCCAGCAGAG TAACCCTTGT 
AATTTGAAAT CTGAAATTAA AAAGTTATCT CAGGGATCTC CAGAACCGAT TGAGCCCAAC 
TTTTTCACAG CAGATTACCA TTTATTACAT CGTTCATCCG GTGGAAACAG CCTGTCCCCA 
AATGACCCTA CAGGTTTACC AACCAGCATT GAATTGGAGG AAGGAATAAC ATATGAACAG 
ATGCAGACTG TGATTGAAGA AGTCCTTGAG GAAAGTGGCT ATTACAATTT TACATCTAAC 
AGGTATCATT CCTATCCATG GGGGACCAAG AATCACCCAA CCAAAAGATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NEK10 ( XM_017005772.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016861261.1 Homo sapiens NIMA related kinase 10 (NEK10), transcript variant X17, mRNA.

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CloneID	OHu93838
Clone ID Related Accession (Same CDS sequence)	XM_017005772.2 , XM_017005772.1
Accession Version	XM_017005772.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3291bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	serine/threonine-protein kinase Nek10 isoform X9
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000003.12) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCTGATC AAGATAAAAA GGTGAAGACC ACAGAAAAAT CAACTGATAA ACAGCAAGAA 
ATCACCATCA GGGACTATTC AGATCTTAAA AGACTTCGGT GCCTTTTGAA CGTCCAATCA 
AGCAAACAAC AGCTTCCAGC CATTAACTTC GATAGTGCCC AAAATAGCAT GACGAAGTCT 
GAGCCCGCCA TCAGGGCGGG TGGACACAGA GCTCGGGGTC AGTGGCATGA ATCCACAGAA 
GCTGTTGAAC TTGAAAATTT TAGTATAAAC TACAAGAATG AGAGAAATTT CAGCAAACAT 
CCTCAGCGTA AACTATTTCA GGAGATCTTT ACCGCCTTGG TGAAAAATAG ACTCATAAGC 
AGAGAGTGGG TTAATCGAGC CCCATCTATT CATTTTCTGA GAGTGTTAAT CTGTCTGAGG 
CTACTAATGA GGGATCCATG TTATCAGGAA ATACTCCATA GCTTGGGTGG GATTGAAAAC 
CTAGCTCAGT ATATGGAGAT TGTAGCCAAT GAGTACCTCG GCTATGGAGA AGAGCAGCAC 
ACTGTGGACA AGCTGGTCAA CATGACATAT ATTTTTCAAA AACTTGCTGC AGTCAAAGAT 
CAAAGAGAAT GGGTCACCAC AAGTGGAGCC CACAAGACAT TAGTAAATTT ACTTGGTGCC 
CGAGATACTA ATGTTCTATT GGGTTCCCTT CTGGCTCTGG CTAGTTTAGC AGAAAGACTA 
ACAGCGGAGT TGCTGCGCCT ACTTTGTGCA GAGCCCCAGG TGAAAGAGCA GGTGAAGCTC 
TATGAGGGGA TACCGGTCCT CCTCAGTCTG CTCCACTCTG ACCACTTGAA GCTCCTCTGG 
AGCATTGTCT GGATTCTGGT ACAGGTTTGT GAGGACCCTG AGACCAGCGT GGAAATTCGC 
ATTTGGGGAG GCATCAAACA GCTTCTTCAT ATTTTACAAG GAGACAGAAA TTTTGTTTCT 
GATCACTCCT CCATTGGAAG CCTGTCCAGT GCAAATGCTG CAGGCCGAAT CCAGCAGCTT 
CATTTATCAG AAGACTTGAG CCCTAGGGAA ATACAAGAAA ATACTTTCTC ACTTCAAGCA 
GCCTGCTGTG CTGCCCTCAC TGAGCTGGTG CTCAATGACA CCAATGCCCA CCAGGTGGTT 
CAGGAAAATG GTGTATATAC AATAGCAAAA TTAATTTTAC CAAATAAGCA AAAGAATGCA 
GCAAAAAGTA ATCTATTACA GTGTTATGCT TTCAGAGCCT TGAGATTTCT CTTCAGTATG 
GAAAGAAACA GACCACTCTT TAAAAGACTT TTCCCCACAG ACTTGTTTGA GATCTTCATT 
GACATAGGGC ATTATGTACG TGATATCAGT GCTTATGAAG AATTGGTATC CAAGCTGAAT 
TTATTAGTGG AGGATGAACT GAAGCAAATT GCTGAAAATA TTGAAAGCAT TAATCAGAAC 
AAAGCTCCTT TGAAATATAT AGGCAACTAT GCAATTTTGG ATCATCTTGG AAGTGGAGCT 
TTTGGCTGTG TTTACAAGGT TAGAAAGCAT AGTGGTCAAA ATCTTTTAGC AATGAAAGAG 
GTCAATTTAC ATAACCCAGC ATTTGGAAAG GATAAGAAAG ATCGAGACAG CAGCGTAAGG 
AATATTGTTT CTGAATTAAC AATAATTAAA GAGCAGCTTT ATCATCCCAA CATTGTACGT 
TATTACAAAA CATTTCTGGA AAATGATAGG TTGTACATAG TTATGGAGCT GATAGAAGGA 
GCCCCGCTTG GAGAGCATTT CAGTTCTTTG AAGGAAAAAC ATCACCATTT TACTGAAGAA 
AGACTATGGA AAATATTTAT ACAGCTGTGC TTAGCTCTTC GATACTTACA CAAGGAGAAG 
AGGATTGTCC ATAGAGATCT GACACCAAAC AACATTATGT TGGGGGATAA GGACAAAGTA 
ACCGTTACTG ACTTTGGCCT GGCAAAGCAA AAACAAGAAA ACAGTAAACT CACCTCTGTG 
GTTGGAACAA TCCTGTATTC TTGCCCCGAG GTACTGAAGA GTGAGCCGTA TGGGGAGAAG 
GCTGATGTCT GGGCAGTAGG CTGCATCCTT TATCAGATGG CGACTTTGAG TCCCCCCTTC 
TACAGCACTA ACATGCTGTC CTTGGCTACA AAAATAGTGG AGGCGGTATA TGAACCAGTC 
CCAGAAGGTA TCTACTCTGA AAAAGTAACA GACACCATCA GCAGGTGCCT CACTCCTGAT 
GCGGAAGCTC GTCCAGATAT TGTAGAAGTC AGTTCGATGA TATCAGATGT CATGATGAAA 
TATTTAGACA ACTTATCTAC ATCCCAGTTG TCCTTGGAAA AGAAGCTAGA ACGGGAACGA 
AGACGCACAC AAAGGTATTT TATGGAAGCC AACCGGAACA CCGTCACATG TCACCATGAG 
CTGGCTGTTC TATCTCACGA GACCTTTGAG AAGGCAAGTT TGAGTAGCAG CAGCAGTGGA 
GCAGCCAGCC TGAAAAGTGA ACTTTCAGAA AGCGCAGACC TGCCCCCTGA AGGCTTCCAG 
GCCTCCTATG GTAAAGACGA AGACAGGGCC TGTGACGAAA TCCTGTCAGA TGATAACTTC 
AACCTGGAAA ATGCTGAGAA AGATACATAT TCAGAGGTAG ATGATGAATT GGACATTTCG 
GATAACTCCA GCAGCTCCAG TTCAAGCCCT CTGAAAGAAT CTACATTCAA CATTTTAAAG 
AGAAGTTTTA GTGCTTCAGG AGGAGAAAGA CAATCCCAAA CAAGGGACTT CACTGGAGGA 
ACAGGATCAA GACCAAGACC AGGGCCACAG ATGGGCACAT TCTTGTGGCA AGCATCAGCA 
GGAATTGCTG TGTCCCAGAG GAAAGTGCGT CAGATCAGTG ATCCTATTCA GCAGATATTA 
ATTCAGCTGC ACAAAATAAT CTATATCACA CAGCTTCCTC CAGCTTTGCA CCACAATTTG 
AAAAGAAGGG TTATAGAGAG ATTCAAGAAA TCCCTCTTCA GCCAGCAGAG TAACCCTTGT 
AATTTGAAAT CTGAAATTAA AAAGTTATCT CAGGGATCTC CAGAACCGAT TGAGCCCAAC 
TTTTTCACAG CAGATTACCA TTTATTACAT CGTTCATCCG GTGGAAACAG CCTGTCCCCA 
AATGACCCTA CAGGTTTACC AACCAGCATT GAATTGGAGG AAGGAATAAC ATATGAACAG 
ATGCAGACTG TGATTGAAGA AGTCCTTGAG GAAAGTGGCT ATTACAATTT TACATCTAAC 
AGGTATCATT CCTATCCATG GGGGACCAAG AATCACCCAA CCAAAAGATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016861261.1
CDS	227..3517
Translation	MPDQDKKVKTTEKSTDKQQEITIRDYSDLKRLRCLLNVQSSKQQLPAINFDSAQNSMTKSEPAIRAGGHRARGQWHESTEAVELENFSINYKNERNFSKHPQRKLFQEIFTALVKNRLISREWVNRAPSIHFLRVLICLRLLMRDPCYQEILHSLGGIENLAQYMEIVANEYLGYGEEQHTVDKLVNMTYIFQKLAAVKDQREWVTTSGAHKTLVNLLGARDTNVLLGSLLALASLAERLTAELLRLLCAEPQVKEQVKLYEGIPVLLSLLHSDHLKLLWSIVWILVQVCEDPETSVEIRIWGGIKQLLHILQGDRNFVSDHSSIGSLSSANAAGRIQQLHLSEDLSPREIQENTFSLQAACCAALTELVLNDTNAHQVVQENGVYTIAKLILPNKQKNAAKSNLLQCYAFRALRFLFSMERNRPLFKRLFPTDLFEIFIDIGHYVRDISAYEELVSKLNLLVEDELKQIAENIESINQNKAPLKYIGNYAILDHLGSGAFGCVYKVRKHSGQNLLAMKEVNLHNPAFGKDKKDRDSSVRNIVSELTIIKEQLYHPNIVRYYKTFLENDRLYIVMELIEGAPLGEHFSSLKEKHHHFTEERLWKIFIQLCLALRYLHKEKRIVHRDLTPNNIMLGDKDKVTVTDFGLAKQKQENSKLTSVVGTILYSCPEVLKSEPYGEKADVWAVGCILYQMATLSPPFYSTNMLSLATKIVEAVYEPVPEGIYSEKVTDTISRCLTPDAEARPDIVEVSSMISDVMMKYLDNLSTSQLSLEKKLERERRRTQRYFMEANRNTVTCHHELAVLSHETFEKASLSSSSSGAASLKSELSESADLPPEGFQASYGKDEDRACDEILSDDNFNLENAEKDTYSEVDDELDISDNSSSSSSSPLKESTFNILKRSFSASGGERQSQTRDFTGGTGSRPRPGPQMGTFLWQASAGIAVSQRKVRQISDPIQQILIQLHKIIYITQLPPALHHNLKRRVIERFKKSLFSQQSNPCNLKSEIKKLSQGSPEPIEPNFFTADYHLLHRSSGGNSLSPNDPTGLPTSIELEEGITYEQMQTVIEEVLEESGYYNFTSNRYHSYPWGTKNHPTKR

Target ORF information:

RefSeq Version	XM_017005772.1
Organism	Homo sapiens(human)
Definition	Homo sapiens NIMA related kinase 10 (NEK10), transcript variant X17, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017005772.1

ORF Insert Sequence:

ATGCCTGATC AAGATAAAAA GGTGAAGACC ACAGAAAAAT CAACTGATAA ACAGCAAGAA 
ATCACCATCA GGGACTATTC AGATCTTAAA AGACTTCGGT GCCTTTTGAA CGTCCAATCA 
AGCAAACAAC AGCTTCCAGC CATTAACTTC GATAGTGCCC AAAATAGCAT GACGAAGTCT 
GAGCCCGCCA TCAGGGCGGG TGGACACAGA GCTCGGGGTC AGTGGCATGA ATCCACAGAA 
GCTGTTGAAC TTGAAAATTT TAGTATAAAC TACAAGAATG AGAGAAATTT CAGCAAACAT 
CCTCAGCGTA AACTATTTCA GGAGATCTTT ACCGCCTTGG TGAAAAATAG ACTCATAAGC 
AGAGAGTGGG TTAATCGAGC CCCATCTATT CATTTTCTGA GAGTGTTAAT CTGTCTGAGG 
CTACTAATGA GGGATCCATG TTATCAGGAA ATACTCCATA GCTTGGGTGG GATTGAAAAC 
CTAGCTCAGT ATATGGAGAT TGTAGCCAAT GAGTACCTCG GCTATGGAGA AGAGCAGCAC 
ACTGTGGACA AGCTGGTCAA CATGACATAT ATTTTTCAAA AACTTGCTGC AGTCAAAGAT 
CAAAGAGAAT GGGTCACCAC AAGTGGAGCC CACAAGACAT TAGTAAATTT ACTTGGTGCC 
CGAGATACTA ATGTTCTATT GGGTTCCCTT CTGGCTCTGG CTAGTTTAGC AGAAAGACTA 
ACAGCGGAGT TGCTGCGCCT ACTTTGTGCA GAGCCCCAGG TGAAAGAGCA GGTGAAGCTC 
TATGAGGGGA TACCGGTCCT CCTCAGTCTG CTCCACTCTG ACCACTTGAA GCTCCTCTGG 
AGCATTGTCT GGATTCTGGT ACAGGTTTGT GAGGACCCTG AGACCAGCGT GGAAATTCGC 
ATTTGGGGAG GCATCAAACA GCTTCTTCAT ATTTTACAAG GAGACAGAAA TTTTGTTTCT 
GATCACTCCT CCATTGGAAG CCTGTCCAGT GCAAATGCTG CAGGCCGAAT CCAGCAGCTT 
CATTTATCAG AAGACTTGAG CCCTAGGGAA ATACAAGAAA ATACTTTCTC ACTTCAAGCA 
GCCTGCTGTG CTGCCCTCAC TGAGCTGGTG CTCAATGACA CCAATGCCCA CCAGGTGGTT 
CAGGAAAATG GTGTATATAC AATAGCAAAA TTAATTTTAC CAAATAAGCA AAAGAATGCA 
GCAAAAAGTA ATCTATTACA GTGTTATGCT TTCAGAGCCT TGAGATTTCT CTTCAGTATG 
GAAAGAAACA GACCACTCTT TAAAAGACTT TTCCCCACAG ACTTGTTTGA GATCTTCATT 
GACATAGGGC ATTATGTACG TGATATCAGT GCTTATGAAG AATTGGTATC CAAGCTGAAT 
TTATTAGTGG AGGATGAACT GAAGCAAATT GCTGAAAATA TTGAAAGCAT TAATCAGAAC 
AAAGCTCCTT TGAAATATAT AGGCAACTAT GCAATTTTGG ATCATCTTGG AAGTGGAGCT 
TTTGGCTGTG TTTACAAGGT TAGAAAGCAT AGTGGTCAAA ATCTTTTAGC AATGAAAGAG 
GTCAATTTAC ATAACCCAGC ATTTGGAAAG GATAAGAAAG ATCGAGACAG CAGCGTAAGG 
AATATTGTTT CTGAATTAAC AATAATTAAA GAGCAGCTTT ATCATCCCAA CATTGTACGT 
TATTACAAAA CATTTCTGGA AAATGATAGG TTGTACATAG TTATGGAGCT GATAGAAGGA 
GCCCCGCTTG GAGAGCATTT CAGTTCTTTG AAGGAAAAAC ATCACCATTT TACTGAAGAA 
AGACTATGGA AAATATTTAT ACAGCTGTGC TTAGCTCTTC GATACTTACA CAAGGAGAAG 
AGGATTGTCC ATAGAGATCT GACACCAAAC AACATTATGT TGGGGGATAA GGACAAAGTA 
ACCGTTACTG ACTTTGGCCT GGCAAAGCAA AAACAAGAAA ACAGTAAACT CACCTCTGTG 
GTTGGAACAA TCCTGTATTC TTGCCCCGAG GTACTGAAGA GTGAGCCGTA TGGGGAGAAG 
GCTGATGTCT GGGCAGTAGG CTGCATCCTT TATCAGATGG CGACTTTGAG TCCCCCCTTC 
TACAGCACTA ACATGCTGTC CTTGGCTACA AAAATAGTGG AGGCGGTATA TGAACCAGTC 
CCAGAAGGTA TCTACTCTGA AAAAGTAACA GACACCATCA GCAGGTGCCT CACTCCTGAT 
GCGGAAGCTC GTCCAGATAT TGTAGAAGTC AGTTCGATGA TATCAGATGT CATGATGAAA 
TATTTAGACA ACTTATCTAC ATCCCAGTTG TCCTTGGAAA AGAAGCTAGA ACGGGAACGA 
AGACGCACAC AAAGGTATTT TATGGAAGCC AACCGGAACA CCGTCACATG TCACCATGAG 
CTGGCTGTTC TATCTCACGA GACCTTTGAG AAGGCAAGTT TGAGTAGCAG CAGCAGTGGA 
GCAGCCAGCC TGAAAAGTGA ACTTTCAGAA AGCGCAGACC TGCCCCCTGA AGGCTTCCAG 
GCCTCCTATG GTAAAGACGA AGACAGGGCC TGTGACGAAA TCCTGTCAGA TGATAACTTC 
AACCTGGAAA ATGCTGAGAA AGATACATAT TCAGAGGTAG ATGATGAATT GGACATTTCG 
GATAACTCCA GCAGCTCCAG TTCAAGCCCT CTGAAAGAAT CTACATTCAA CATTTTAAAG 
AGAAGTTTTA GTGCTTCAGG AGGAGAAAGA CAATCCCAAA CAAGGGACTT CACTGGAGGA 
ACAGGATCAA GACCAAGACC AGGGCCACAG ATGGGCACAT TCTTGTGGCA AGCATCAGCA 
GGAATTGCTG TGTCCCAGAG GAAAGTGCGT CAGATCAGTG ATCCTATTCA GCAGATATTA 
ATTCAGCTGC ACAAAATAAT CTATATCACA CAGCTTCCTC CAGCTTTGCA CCACAATTTG 
AAAAGAAGGG TTATAGAGAG ATTCAAGAAA TCCCTCTTCA GCCAGCAGAG TAACCCTTGT 
AATTTGAAAT CTGAAATTAA AAAGTTATCT CAGGGATCTC CAGAACCGAT TGAGCCCAAC 
TTTTTCACAG CAGATTACCA TTTATTACAT CGTTCATCCG GTGGAAACAG CCTGTCCCCA 
AATGACCCTA CAGGTTTACC AACCAGCATT GAATTGGAGG AAGGAATAAC ATATGAACAG 
ATGCAGACTG TGATTGAAGA AGTCCTTGAG GAAAGTGGCT ATTACAATTT TACATCTAAC 
AGGTATCATT CCTATCCATG GGGGACCAAG AATCACCCAA CCAAAAGATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NEK10 ( NM_152534.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_689747.3 Homo sapiens NIMA related kinase 10 (NEK10), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu36991
Clone ID Related Accession (Same CDS sequence)	XM_017005764.1 , XM_017005762.2 , NM_152534.4 , XM_017005761.1 , XM_017005763.1 , XM_017005762.1 , XM_017005761.2 , XM_017005765.1
Accession Version	NM_152534.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3519bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-29
Organism	Homo sapiens(human)
Product	serine/threonine-protein kinase Nek10 isoform 2
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from DB094082.1, AK123061.1, AK098832.1, AC093555.2, BC045758.1, AC098931.3 and AK057247.1. On or before Jan 22, 2015 this sequence version replaced XM_006712994.1, NM_152534.3. Transcript Variant: This variant (2) represents the inferred longest transcript and encodes the longest isoform (2). ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## inferred exon combination :: based on alignments, homology RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGCCTGATC AAGATAAAAA GGTGAAGACC ACAGAAAAAT CAACTGATAA ACAGCAAGAA 
ATCACCATCA GGGACTATTC AGATCTTAAA AGACTTCGGT GCCTTTTGAA CGTCCAATCA 
AGCAAACAAC AGCTTCCAGC CATTAACTTC GATAGTGCCC AAAATAGCAT GACGAAGTCT 
GAGCCCGCCA TCAGGGCGGG TGGACACAGA GCTCGGGGTC AGTGGCATGA ATCCACAGAA 
GCTGTTGAAC TTGAAAATTT TAGTATAAAC TACAAGAATG AGAGAAATTT CAGCAAACAT 
CCTCAGCGTA AACTATTTCA GGAGATCTTT ACCGCCTTGG TGAAAAATAG ACTCATAAGC 
AGAGAGTGGG TTAATCGAGC CCCATCTATT CATTTTCTGA GAGTGTTAAT CTGTCTGAGG 
CTACTAATGA GGGATCCATG TTATCAGGAA ATACTCCATA GCTTGGGTGG GATTGAAAAC 
CTAGCTCAGT ATATGGAGAT TGTAGCCAAT GAGTACCTCG GCTATGGAGA AGAGCAGCAC 
ACTGTGGACA AGCTGGTCAA CATGACATAT ATTTTTCAAA AACTTGCTGC AGTCAAAGAT 
CAAAGAGAAT GGGTCACCAC AAGTGGAGCC CACAAGACAT TAGTAAATTT ACTTGGTGCC 
CGAGATACTA ATGTTCTATT GGGTTCCCTT CTGGCTCTGG CTAGTTTAGC AGAAAGTCAA 
GAATGTAGGG AGAAGATAAG TGAACTCAAC ATTGTAGAAA ATCTGTTGAT GATTTTACAT 
GAATATGACT TGCTTTCTAA AAGACTAACA GCGGAGTTGC TGCGCCTACT TTGTGCAGAG 
CCCCAGGTGA AAGAGCAGGT GAAGCTCTAT GAGGGGATAC CGGTCCTCCT CAGTCTGCTC 
CACTCTGACC ACTTGAAGCT CCTCTGGAGC ATTGTCTGGA TTCTGGTACA GGTTTGTGAG 
GACCCTGAGA CCAGCGTGGA AATTCGCATT TGGGGAGGCA TCAAACAGCT TCTTCATATT 
TTACAAGGAG ACAGAAATTT TGTTTCTGAT CACTCCTCCA TTGGAAGCCT GTCCAGTGCA 
AATGCTGCAG GCCGAATCCA GCAGCTTCAT TTATCAGAAG ACTTGAGCCC TAGGGAAATA 
CAAGAAAATA CTTTCTCACT TCAAGCAGCC TGCTGTGCTG CCCTCACTGA GCTGGTGCTC 
AATGACACCA ATGCCCACCA GGTGGTTCAG GAAAATGGTG TATATACAAT AGCAAAATTA 
ATTTTACCAA ATAAGCAAAA GAATGCAGCA AAAAGTAATC TATTACAGTG TTATGCTTTC 
AGAGCCTTGA GATTTCTCTT CAGTATGGAA AGAAACAGAC CACTCTTTAA AAGACTTTTC 
CCCACAGACT TGTTTGAGAT CTTCATTGAC ATAGGGCATT ATGTACGTGA TATCAGTGCT 
TATGAAGAAT TGGTATCCAA GCTGAATTTA TTAGTGGAGG ATGAACTGAA GCAAATTGCT 
GAAAATATTG AAAGCATTAA TCAGAACAAA GCTCCTTTGA AATATATAGG CAACTATGCA 
ATTTTGGATC ATCTTGGAAG TGGAGCTTTT GGCTGTGTTT ACAAGGTTAG AAAGCATAGT 
GGTCAAAATC TTTTAGCAAT GAAAGAGGTC AATTTACATA ACCCAGCATT TGGAAAGGAT 
AAGAAAGATC GAGACAGCAG CGTAAGGAAT ATTGTTTCTG AATTAACAAT AATTAAAGAG 
CAGCTTTATC ATCCCAACAT TGTACGTTAT TACAAAACAT TTCTGGAAAA TGATAGGTTG 
TACATAGTTA TGGAGCTGAT AGAAGGAGCC CCGCTTGGAG AGCATTTCAG TTCTTTGAAG 
GAAAAACATC ACCATTTTAC TGAAGAAAGA CTATGGAAAA TATTTATACA GCTGTGCTTA 
GCTCTTCGAT ACTTACACAA GGAGAAGAGG ATTGTCCATA GAGATCTGAC ACCAAACAAC 
ATTATGTTGG GGGATAAGGA CAAAGTAACC GTTACTGACT TTGGCCTGGC AAAGCAAAAA 
CAAGAAAACA GTAAACTCAC CTCTGTGGTT GGAACAATCC TGTATTCTTG CCCCGAGGTA 
CTGAAGAGTG AGCCGTATGG GGAGAAGGCT GATGTCTGGG CAGTAGGCTG CATCCTTTAT 
CAGATGGCGA CTTTGAGTCC CCCCTTCTAC AGCACTAACA TGCTGTCCTT GGCTACAAAA 
ATAGTGGAGG CGGTATATGA ACCAGTCCCA GAAGGTATCT ACTCTGAAAA AGTAACAGAC 
ACCATCAGCA GGTGCCTCAC TCCTGATGCG GAAGCTCGTC CAGATATTGT AGAAGTCAGT 
TCGATGATAT CAGATGTCAT GATGAAATAT TTAGACAACT TATCTACATC CCAGTTGTCC 
TTGGAAAAGA AGCTAGAACG GGAACGAAGA CGCACACAAA GGTATTTTAT GGAAGCCAAC 
CGGAACACCG TCACATGTCA CCATGAGCTG GCTGTTCTAT CTCACGAGAC CTTTGAGAAG 
GCAAGTTTGA GTAGCAGCAG CAGTGGAGCA GCCAGCCTGA AAAGTGAACT TTCAGAAAGC 
GCAGACCTGC CCCCTGAAGG CTTCCAGGCC TCCTATGGTA AAGACGAAGA CAGGGCCTGT 
GACGAAATCC TGTCAGATGA TAACTTCAAC CTGGAAAATG CTGAGAAAGA TACATATTCA 
GAGGTAGATG ATGAATTGGA CATTTCGGAT AACTCCAGCA GCTCCAGTTC AAGCCCTCTG 
AAAGAATCTA CATTCAACAT TTTAAAGAGA AGTTTTAGTG CTTCAGGAGG AGAAAGACAA 
TCCCAAACAA GGGACTTCAC TGGAGGAACA GGATCAAGAC CAAGACCAGC TTTGCTGCCT 
CTTGACCTGC TTCTGAAAGT GCCACCCCAC ATGCTCAGGG CCCACATTAA GGAAATAGAG 
GCTGAGTTAG TGACAGGGTG GCAGTCCCAT AGCCTTCCTG CTGTGATTCT TCGAAATCTC 
AAAGATCATG GGCCACAGAT GGGCACATTC TTGTGGCAAG CATCAGCAGG AATTGCTGTG 
TCCCAGAGGA AAGTGCGTCA GATCAGTGAT CCTATTCAGC AGATATTAAT TCAGCTGCAC 
AAAATAATCT ATATCACACA GCTTCCTCCA GCTTTGCACC ACAATTTGAA AAGAAGGGTT 
ATAGAGAGAT TCAAGAAATC CCTCTTCAGC CAGCAGAGTA ACCCTTGTAA TTTGAAATCT 
GAAATTAAAA AGTTATCTCA GGGATCTCCA GAACCGATTG AGCCCAACTT TTTCACAGCA 
GATTACCATT TATTACATCG TTCATCCGGT GGAAACAGCC TGTCCCCAAA TGACCCTACA 
GGTTTACCAA CCAGCATTGA ATTGGAGGAA GGAATAACAT ATGAACAGAT GCAGACTGTG 
ATTGAAGAAG TCCTTGAGGA AAGTGGCTAT TACAATTTTA CATCTAACAG GTATCATTCC 
TATCCATGGG GGACCAAGAA TCACCCAACC AAAAGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_689747.3
CDS	314..3832
Translation	MPDQDKKVKTTEKSTDKQQEITIRDYSDLKRLRCLLNVQSSKQQLPAINFDSAQNSMTKSEPAIRAGGHRARGQWHESTEAVELENFSINYKNERNFSKHPQRKLFQEIFTALVKNRLISREWVNRAPSIHFLRVLICLRLLMRDPCYQEILHSLGGIENLAQYMEIVANEYLGYGEEQHTVDKLVNMTYIFQKLAAVKDQREWVTTSGAHKTLVNLLGARDTNVLLGSLLALASLAESQECREKISELNIVENLLMILHEYDLLSKRLTAELLRLLCAEPQVKEQVKLYEGIPVLLSLLHSDHLKLLWSIVWILVQVCEDPETSVEIRIWGGIKQLLHILQGDRNFVSDHSSIGSLSSANAAGRIQQLHLSEDLSPREIQENTFSLQAACCAALTELVLNDTNAHQVVQENGVYTIAKLILPNKQKNAAKSNLLQCYAFRALRFLFSMERNRPLFKRLFPTDLFEIFIDIGHYVRDISAYEELVSKLNLLVEDELKQIAENIESINQNKAPLKYIGNYAILDHLGSGAFGCVYKVRKHSGQNLLAMKEVNLHNPAFGKDKKDRDSSVRNIVSELTIIKEQLYHPNIVRYYKTFLENDRLYIVMELIEGAPLGEHFSSLKEKHHHFTEERLWKIFIQLCLALRYLHKEKRIVHRDLTPNNIMLGDKDKVTVTDFGLAKQKQENSKLTSVVGTILYSCPEVLKSEPYGEKADVWAVGCILYQMATLSPPFYSTNMLSLATKIVEAVYEPVPEGIYSEKVTDTISRCLTPDAEARPDIVEVSSMISDVMMKYLDNLSTSQLSLEKKLERERRRTQRYFMEANRNTVTCHHELAVLSHETFEKASLSSSSSGAASLKSELSESADLPPEGFQASYGKDEDRACDEILSDDNFNLENAEKDTYSEVDDELDISDNSSSSSSSPLKESTFNILKRSFSASGGERQSQTRDFTGGTGSRPRPALLPLDLLLKVPPHMLRAHIKEIEAELVTGWQSHSLPAVILRNLKDHGPQMGTFLWQASAGIAVSQRKVRQISDPIQQILIQLHKIIYITQLPPALHHNLKRRVIERFKKSLFSQQSNPCNLKSEIKKLSQGSPEPIEPNFFTADYHLLHRSSGGNSLSPNDPTGLPTSIELEEGITYEQMQTVIEEVLEESGYYNFTSNRYHSYPWGTKNHPTKR

Target ORF information:

RefSeq Version	NM_152534.4
Organism	Homo sapiens(human)
Definition	Homo sapiens NIMA related kinase 10 (NEK10), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_152534.4

ORF Insert Sequence:

ATGCCTGATC AAGATAAAAA GGTGAAGACC ACAGAAAAAT CAACTGATAA ACAGCAAGAA 
ATCACCATCA GGGACTATTC AGATCTTAAA AGACTTCGGT GCCTTTTGAA CGTCCAATCA 
AGCAAACAAC AGCTTCCAGC CATTAACTTC GATAGTGCCC AAAATAGCAT GACGAAGTCT 
GAGCCCGCCA TCAGGGCGGG TGGACACAGA GCTCGGGGTC AGTGGCATGA ATCCACAGAA 
GCTGTTGAAC TTGAAAATTT TAGTATAAAC TACAAGAATG AGAGAAATTT CAGCAAACAT 
CCTCAGCGTA AACTATTTCA GGAGATCTTT ACCGCCTTGG TGAAAAATAG ACTCATAAGC 
AGAGAGTGGG TTAATCGAGC CCCATCTATT CATTTTCTGA GAGTGTTAAT CTGTCTGAGG 
CTACTAATGA GGGATCCATG TTATCAGGAA ATACTCCATA GCTTGGGTGG GATTGAAAAC 
CTAGCTCAGT ATATGGAGAT TGTAGCCAAT GAGTACCTCG GCTATGGAGA AGAGCAGCAC 
ACTGTGGACA AGCTGGTCAA CATGACATAT ATTTTTCAAA AACTTGCTGC AGTCAAAGAT 
CAAAGAGAAT GGGTCACCAC AAGTGGAGCC CACAAGACAT TAGTAAATTT ACTTGGTGCC 
CGAGATACTA ATGTTCTATT GGGTTCCCTT CTGGCTCTGG CTAGTTTAGC AGAAAGTCAA 
GAATGTAGGG AGAAGATAAG TGAACTCAAC ATTGTAGAAA ATCTGTTGAT GATTTTACAT 
GAATATGACT TGCTTTCTAA AAGACTAACA GCGGAGTTGC TGCGCCTACT TTGTGCAGAG 
CCCCAGGTGA AAGAGCAGGT GAAGCTCTAT GAGGGGATAC CGGTCCTCCT CAGTCTGCTC 
CACTCTGACC ACTTGAAGCT CCTCTGGAGC ATTGTCTGGA TTCTGGTACA GGTTTGTGAG 
GACCCTGAGA CCAGCGTGGA AATTCGCATT TGGGGAGGCA TCAAACAGCT TCTTCATATT 
TTACAAGGAG ACAGAAATTT TGTTTCTGAT CACTCCTCCA TTGGAAGCCT GTCCAGTGCA 
AATGCTGCAG GCCGAATCCA GCAGCTTCAT TTATCAGAAG ACTTGAGCCC TAGGGAAATA 
CAAGAAAATA CTTTCTCACT TCAAGCAGCC TGCTGTGCTG CCCTCACTGA GCTGGTGCTC 
AATGACACCA ATGCCCACCA GGTGGTTCAG GAAAATGGTG TATATACAAT AGCAAAATTA 
ATTTTACCAA ATAAGCAAAA GAATGCAGCA AAAAGTAATC TATTACAGTG TTATGCTTTC 
AGAGCCTTGA GATTTCTCTT CAGTATGGAA AGAAACAGAC CACTCTTTAA AAGACTTTTC 
CCCACAGACT TGTTTGAGAT CTTCATTGAC ATAGGGCATT ATGTACGTGA TATCAGTGCT 
TATGAAGAAT TGGTATCCAA GCTGAATTTA TTAGTGGAGG ATGAACTGAA GCAAATTGCT 
GAAAATATTG AAAGCATTAA TCAGAACAAA GCTCCTTTGA AATATATAGG CAACTATGCA 
ATTTTGGATC ATCTTGGAAG TGGAGCTTTT GGCTGTGTTT ACAAGGTTAG AAAGCATAGT 
GGTCAAAATC TTTTAGCAAT GAAAGAGGTC AATTTACATA ACCCAGCATT TGGAAAGGAT 
AAGAAAGATC GAGACAGCAG CGTAAGGAAT ATTGTTTCTG AATTAACAAT AATTAAAGAG 
CAGCTTTATC ATCCCAACAT TGTACGTTAT TACAAAACAT TTCTGGAAAA TGATAGGTTG 
TACATAGTTA TGGAGCTGAT AGAAGGAGCC CCGCTTGGAG AGCATTTCAG TTCTTTGAAG 
GAAAAACATC ACCATTTTAC TGAAGAAAGA CTATGGAAAA TATTTATACA GCTGTGCTTA 
GCTCTTCGAT ACTTACACAA GGAGAAGAGG ATTGTCCATA GAGATCTGAC ACCAAACAAC 
ATTATGTTGG GGGATAAGGA CAAAGTAACC GTTACTGACT TTGGCCTGGC AAAGCAAAAA 
CAAGAAAACA GTAAACTCAC CTCTGTGGTT GGAACAATCC TGTATTCTTG CCCCGAGGTA 
CTGAAGAGTG AGCCGTATGG GGAGAAGGCT GATGTCTGGG CAGTAGGCTG CATCCTTTAT 
CAGATGGCGA CTTTGAGTCC CCCCTTCTAC AGCACTAACA TGCTGTCCTT GGCTACAAAA 
ATAGTGGAGG CGGTATATGA ACCAGTCCCA GAAGGTATCT ACTCTGAAAA AGTAACAGAC 
ACCATCAGCA GGTGCCTCAC TCCTGATGCG GAAGCTCGTC CAGATATTGT AGAAGTCAGT 
TCGATGATAT CAGATGTCAT GATGAAATAT TTAGACAACT TATCTACATC CCAGTTGTCC 
TTGGAAAAGA AGCTAGAACG GGAACGAAGA CGCACACAAA GGTATTTTAT GGAAGCCAAC 
CGGAACACCG TCACATGTCA CCATGAGCTG GCTGTTCTAT CTCACGAGAC CTTTGAGAAG 
GCAAGTTTGA GTAGCAGCAG CAGTGGAGCA GCCAGCCTGA AAAGTGAACT TTCAGAAAGC 
GCAGACCTGC CCCCTGAAGG CTTCCAGGCC TCCTATGGTA AAGACGAAGA CAGGGCCTGT 
GACGAAATCC TGTCAGATGA TAACTTCAAC CTGGAAAATG CTGAGAAAGA TACATATTCA 
GAGGTAGATG ATGAATTGGA CATTTCGGAT AACTCCAGCA GCTCCAGTTC AAGCCCTCTG 
AAAGAATCTA CATTCAACAT TTTAAAGAGA AGTTTTAGTG CTTCAGGAGG AGAAAGACAA 
TCCCAAACAA GGGACTTCAC TGGAGGAACA GGATCAAGAC CAAGACCAGC TTTGCTGCCT 
CTTGACCTGC TTCTGAAAGT GCCACCCCAC ATGCTCAGGG CCCACATTAA GGAAATAGAG 
GCTGAGTTAG TGACAGGGTG GCAGTCCCAT AGCCTTCCTG CTGTGATTCT TCGAAATCTC 
AAAGATCATG GGCCACAGAT GGGCACATTC TTGTGGCAAG CATCAGCAGG AATTGCTGTG 
TCCCAGAGGA AAGTGCGTCA GATCAGTGAT CCTATTCAGC AGATATTAAT TCAGCTGCAC 
AAAATAATCT ATATCACACA GCTTCCTCCA GCTTTGCACC ACAATTTGAA AAGAAGGGTT 
ATAGAGAGAT TCAAGAAATC CCTCTTCAGC CAGCAGAGTA ACCCTTGTAA TTTGAAATCT 
GAAATTAAAA AGTTATCTCA GGGATCTCCA GAACCGATTG AGCCCAACTT TTTCACAGCA 
GATTACCATT TATTACATCG TTCATCCGGT GGAAACAGCC TGTCCCCAAA TGACCCTACA 
GGTTTACCAA CCAGCATTGA ATTGGAGGAA GGAATAACAT ATGAACAGAT GCAGACTGTG 
ATTGAAGAAG TCCTTGAGGA AAGTGGCTAT TACAATTTTA CATCTAACAG GTATCATTCC 
TATCCATGGG GGACCAAGAA TCACCCAACC AAAAGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NEK10 ( XM_017005764.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016861253.1 Homo sapiens NIMA related kinase 10 (NEK10), transcript variant X5, mRNA.

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CloneID	OHu36991
Clone ID Related Accession (Same CDS sequence)	XM_017005764.1 , XM_017005762.2 , NM_152534.4 , XM_017005761.1 , XM_017005763.1 , XM_017005762.1 , XM_017005761.2 , XM_017005765.1
Accession Version	XM_017005764.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3519bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	serine/threonine-protein kinase Nek10 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000003.12) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCTGATC AAGATAAAAA GGTGAAGACC ACAGAAAAAT CAACTGATAA ACAGCAAGAA 
ATCACCATCA GGGACTATTC AGATCTTAAA AGACTTCGGT GCCTTTTGAA CGTCCAATCA 
AGCAAACAAC AGCTTCCAGC CATTAACTTC GATAGTGCCC AAAATAGCAT GACGAAGTCT 
GAGCCCGCCA TCAGGGCGGG TGGACACAGA GCTCGGGGTC AGTGGCATGA ATCCACAGAA 
GCTGTTGAAC TTGAAAATTT TAGTATAAAC TACAAGAATG AGAGAAATTT CAGCAAACAT 
CCTCAGCGTA AACTATTTCA GGAGATCTTT ACCGCCTTGG TGAAAAATAG ACTCATAAGC 
AGAGAGTGGG TTAATCGAGC CCCATCTATT CATTTTCTGA GAGTGTTAAT CTGTCTGAGG 
CTACTAATGA GGGATCCATG TTATCAGGAA ATACTCCATA GCTTGGGTGG GATTGAAAAC 
CTAGCTCAGT ATATGGAGAT TGTAGCCAAT GAGTACCTCG GCTATGGAGA AGAGCAGCAC 
ACTGTGGACA AGCTGGTCAA CATGACATAT ATTTTTCAAA AACTTGCTGC AGTCAAAGAT 
CAAAGAGAAT GGGTCACCAC AAGTGGAGCC CACAAGACAT TAGTAAATTT ACTTGGTGCC 
CGAGATACTA ATGTTCTATT GGGTTCCCTT CTGGCTCTGG CTAGTTTAGC AGAAAGTCAA 
GAATGTAGGG AGAAGATAAG TGAACTCAAC ATTGTAGAAA ATCTGTTGAT GATTTTACAT 
GAATATGACT TGCTTTCTAA AAGACTAACA GCGGAGTTGC TGCGCCTACT TTGTGCAGAG 
CCCCAGGTGA AAGAGCAGGT GAAGCTCTAT GAGGGGATAC CGGTCCTCCT CAGTCTGCTC 
CACTCTGACC ACTTGAAGCT CCTCTGGAGC ATTGTCTGGA TTCTGGTACA GGTTTGTGAG 
GACCCTGAGA CCAGCGTGGA AATTCGCATT TGGGGAGGCA TCAAACAGCT TCTTCATATT 
TTACAAGGAG ACAGAAATTT TGTTTCTGAT CACTCCTCCA TTGGAAGCCT GTCCAGTGCA 
AATGCTGCAG GCCGAATCCA GCAGCTTCAT TTATCAGAAG ACTTGAGCCC TAGGGAAATA 
CAAGAAAATA CTTTCTCACT TCAAGCAGCC TGCTGTGCTG CCCTCACTGA GCTGGTGCTC 
AATGACACCA ATGCCCACCA GGTGGTTCAG GAAAATGGTG TATATACAAT AGCAAAATTA 
ATTTTACCAA ATAAGCAAAA GAATGCAGCA AAAAGTAATC TATTACAGTG TTATGCTTTC 
AGAGCCTTGA GATTTCTCTT CAGTATGGAA AGAAACAGAC CACTCTTTAA AAGACTTTTC 
CCCACAGACT TGTTTGAGAT CTTCATTGAC ATAGGGCATT ATGTACGTGA TATCAGTGCT 
TATGAAGAAT TGGTATCCAA GCTGAATTTA TTAGTGGAGG ATGAACTGAA GCAAATTGCT 
GAAAATATTG AAAGCATTAA TCAGAACAAA GCTCCTTTGA AATATATAGG CAACTATGCA 
ATTTTGGATC ATCTTGGAAG TGGAGCTTTT GGCTGTGTTT ACAAGGTTAG AAAGCATAGT 
GGTCAAAATC TTTTAGCAAT GAAAGAGGTC AATTTACATA ACCCAGCATT TGGAAAGGAT 
AAGAAAGATC GAGACAGCAG CGTAAGGAAT ATTGTTTCTG AATTAACAAT AATTAAAGAG 
CAGCTTTATC ATCCCAACAT TGTACGTTAT TACAAAACAT TTCTGGAAAA TGATAGGTTG 
TACATAGTTA TGGAGCTGAT AGAAGGAGCC CCGCTTGGAG AGCATTTCAG TTCTTTGAAG 
GAAAAACATC ACCATTTTAC TGAAGAAAGA CTATGGAAAA TATTTATACA GCTGTGCTTA 
GCTCTTCGAT ACTTACACAA GGAGAAGAGG ATTGTCCATA GAGATCTGAC ACCAAACAAC 
ATTATGTTGG GGGATAAGGA CAAAGTAACC GTTACTGACT TTGGCCTGGC AAAGCAAAAA 
CAAGAAAACA GTAAACTCAC CTCTGTGGTT GGAACAATCC TGTATTCTTG CCCCGAGGTA 
CTGAAGAGTG AGCCGTATGG GGAGAAGGCT GATGTCTGGG CAGTAGGCTG CATCCTTTAT 
CAGATGGCGA CTTTGAGTCC CCCCTTCTAC AGCACTAACA TGCTGTCCTT GGCTACAAAA 
ATAGTGGAGG CGGTATATGA ACCAGTCCCA GAAGGTATCT ACTCTGAAAA AGTAACAGAC 
ACCATCAGCA GGTGCCTCAC TCCTGATGCG GAAGCTCGTC CAGATATTGT AGAAGTCAGT 
TCGATGATAT CAGATGTCAT GATGAAATAT TTAGACAACT TATCTACATC CCAGTTGTCC 
TTGGAAAAGA AGCTAGAACG GGAACGAAGA CGCACACAAA GGTATTTTAT GGAAGCCAAC 
CGGAACACCG TCACATGTCA CCATGAGCTG GCTGTTCTAT CTCACGAGAC CTTTGAGAAG 
GCAAGTTTGA GTAGCAGCAG CAGTGGAGCA GCCAGCCTGA AAAGTGAACT TTCAGAAAGC 
GCAGACCTGC CCCCTGAAGG CTTCCAGGCC TCCTATGGTA AAGACGAAGA CAGGGCCTGT 
GACGAAATCC TGTCAGATGA TAACTTCAAC CTGGAAAATG CTGAGAAAGA TACATATTCA 
GAGGTAGATG ATGAATTGGA CATTTCGGAT AACTCCAGCA GCTCCAGTTC AAGCCCTCTG 
AAAGAATCTA CATTCAACAT TTTAAAGAGA AGTTTTAGTG CTTCAGGAGG AGAAAGACAA 
TCCCAAACAA GGGACTTCAC TGGAGGAACA GGATCAAGAC CAAGACCAGC TTTGCTGCCT 
CTTGACCTGC TTCTGAAAGT GCCACCCCAC ATGCTCAGGG CCCACATTAA GGAAATAGAG 
GCTGAGTTAG TGACAGGGTG GCAGTCCCAT AGCCTTCCTG CTGTGATTCT TCGAAATCTC 
AAAGATCATG GGCCACAGAT GGGCACATTC TTGTGGCAAG CATCAGCAGG AATTGCTGTG 
TCCCAGAGGA AAGTGCGTCA GATCAGTGAT CCTATTCAGC AGATATTAAT TCAGCTGCAC 
AAAATAATCT ATATCACACA GCTTCCTCCA GCTTTGCACC ACAATTTGAA AAGAAGGGTT 
ATAGAGAGAT TCAAGAAATC CCTCTTCAGC CAGCAGAGTA ACCCTTGTAA TTTGAAATCT 
GAAATTAAAA AGTTATCTCA GGGATCTCCA GAACCGATTG AGCCCAACTT TTTCACAGCA 
GATTACCATT TATTACATCG TTCATCCGGT GGAAACAGCC TGTCCCCAAA TGACCCTACA 
GGTTTACCAA CCAGCATTGA ATTGGAGGAA GGAATAACAT ATGAACAGAT GCAGACTGTG 
ATTGAAGAAG TCCTTGAGGA AAGTGGCTAT TACAATTTTA CATCTAACAG GTATCATTCC 
TATCCATGGG GGACCAAGAA TCACCCAACC AAAAGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016861253.1
CDS	204..3722
Translation	MPDQDKKVKTTEKSTDKQQEITIRDYSDLKRLRCLLNVQSSKQQLPAINFDSAQNSMTKSEPAIRAGGHRARGQWHESTEAVELENFSINYKNERNFSKHPQRKLFQEIFTALVKNRLISREWVNRAPSIHFLRVLICLRLLMRDPCYQEILHSLGGIENLAQYMEIVANEYLGYGEEQHTVDKLVNMTYIFQKLAAVKDQREWVTTSGAHKTLVNLLGARDTNVLLGSLLALASLAESQECREKISELNIVENLLMILHEYDLLSKRLTAELLRLLCAEPQVKEQVKLYEGIPVLLSLLHSDHLKLLWSIVWILVQVCEDPETSVEIRIWGGIKQLLHILQGDRNFVSDHSSIGSLSSANAAGRIQQLHLSEDLSPREIQENTFSLQAACCAALTELVLNDTNAHQVVQENGVYTIAKLILPNKQKNAAKSNLLQCYAFRALRFLFSMERNRPLFKRLFPTDLFEIFIDIGHYVRDISAYEELVSKLNLLVEDELKQIAENIESINQNKAPLKYIGNYAILDHLGSGAFGCVYKVRKHSGQNLLAMKEVNLHNPAFGKDKKDRDSSVRNIVSELTIIKEQLYHPNIVRYYKTFLENDRLYIVMELIEGAPLGEHFSSLKEKHHHFTEERLWKIFIQLCLALRYLHKEKRIVHRDLTPNNIMLGDKDKVTVTDFGLAKQKQENSKLTSVVGTILYSCPEVLKSEPYGEKADVWAVGCILYQMATLSPPFYSTNMLSLATKIVEAVYEPVPEGIYSEKVTDTISRCLTPDAEARPDIVEVSSMISDVMMKYLDNLSTSQLSLEKKLERERRRTQRYFMEANRNTVTCHHELAVLSHETFEKASLSSSSSGAASLKSELSESADLPPEGFQASYGKDEDRACDEILSDDNFNLENAEKDTYSEVDDELDISDNSSSSSSSPLKESTFNILKRSFSASGGERQSQTRDFTGGTGSRPRPALLPLDLLLKVPPHMLRAHIKEIEAELVTGWQSHSLPAVILRNLKDHGPQMGTFLWQASAGIAVSQRKVRQISDPIQQILIQLHKIIYITQLPPALHHNLKRRVIERFKKSLFSQQSNPCNLKSEIKKLSQGSPEPIEPNFFTADYHLLHRSSGGNSLSPNDPTGLPTSIELEEGITYEQMQTVIEEVLEESGYYNFTSNRYHSYPWGTKNHPTKR

Target ORF information:

RefSeq Version	XM_017005764.1
Organism	Homo sapiens(human)
Definition	Homo sapiens NIMA related kinase 10 (NEK10), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017005764.1

ORF Insert Sequence:

ATGCCTGATC AAGATAAAAA GGTGAAGACC ACAGAAAAAT CAACTGATAA ACAGCAAGAA 
ATCACCATCA GGGACTATTC AGATCTTAAA AGACTTCGGT GCCTTTTGAA CGTCCAATCA 
AGCAAACAAC AGCTTCCAGC CATTAACTTC GATAGTGCCC AAAATAGCAT GACGAAGTCT 
GAGCCCGCCA TCAGGGCGGG TGGACACAGA GCTCGGGGTC AGTGGCATGA ATCCACAGAA 
GCTGTTGAAC TTGAAAATTT TAGTATAAAC TACAAGAATG AGAGAAATTT CAGCAAACAT 
CCTCAGCGTA AACTATTTCA GGAGATCTTT ACCGCCTTGG TGAAAAATAG ACTCATAAGC 
AGAGAGTGGG TTAATCGAGC CCCATCTATT CATTTTCTGA GAGTGTTAAT CTGTCTGAGG 
CTACTAATGA GGGATCCATG TTATCAGGAA ATACTCCATA GCTTGGGTGG GATTGAAAAC 
CTAGCTCAGT ATATGGAGAT TGTAGCCAAT GAGTACCTCG GCTATGGAGA AGAGCAGCAC 
ACTGTGGACA AGCTGGTCAA CATGACATAT ATTTTTCAAA AACTTGCTGC AGTCAAAGAT 
CAAAGAGAAT GGGTCACCAC AAGTGGAGCC CACAAGACAT TAGTAAATTT ACTTGGTGCC 
CGAGATACTA ATGTTCTATT GGGTTCCCTT CTGGCTCTGG CTAGTTTAGC AGAAAGTCAA 
GAATGTAGGG AGAAGATAAG TGAACTCAAC ATTGTAGAAA ATCTGTTGAT GATTTTACAT 
GAATATGACT TGCTTTCTAA AAGACTAACA GCGGAGTTGC TGCGCCTACT TTGTGCAGAG 
CCCCAGGTGA AAGAGCAGGT GAAGCTCTAT GAGGGGATAC CGGTCCTCCT CAGTCTGCTC 
CACTCTGACC ACTTGAAGCT CCTCTGGAGC ATTGTCTGGA TTCTGGTACA GGTTTGTGAG 
GACCCTGAGA CCAGCGTGGA AATTCGCATT TGGGGAGGCA TCAAACAGCT TCTTCATATT 
TTACAAGGAG ACAGAAATTT TGTTTCTGAT CACTCCTCCA TTGGAAGCCT GTCCAGTGCA 
AATGCTGCAG GCCGAATCCA GCAGCTTCAT TTATCAGAAG ACTTGAGCCC TAGGGAAATA 
CAAGAAAATA CTTTCTCACT TCAAGCAGCC TGCTGTGCTG CCCTCACTGA GCTGGTGCTC 
AATGACACCA ATGCCCACCA GGTGGTTCAG GAAAATGGTG TATATACAAT AGCAAAATTA 
ATTTTACCAA ATAAGCAAAA GAATGCAGCA AAAAGTAATC TATTACAGTG TTATGCTTTC 
AGAGCCTTGA GATTTCTCTT CAGTATGGAA AGAAACAGAC CACTCTTTAA AAGACTTTTC 
CCCACAGACT TGTTTGAGAT CTTCATTGAC ATAGGGCATT ATGTACGTGA TATCAGTGCT 
TATGAAGAAT TGGTATCCAA GCTGAATTTA TTAGTGGAGG ATGAACTGAA GCAAATTGCT 
GAAAATATTG AAAGCATTAA TCAGAACAAA GCTCCTTTGA AATATATAGG CAACTATGCA 
ATTTTGGATC ATCTTGGAAG TGGAGCTTTT GGCTGTGTTT ACAAGGTTAG AAAGCATAGT 
GGTCAAAATC TTTTAGCAAT GAAAGAGGTC AATTTACATA ACCCAGCATT TGGAAAGGAT 
AAGAAAGATC GAGACAGCAG CGTAAGGAAT ATTGTTTCTG AATTAACAAT AATTAAAGAG 
CAGCTTTATC ATCCCAACAT TGTACGTTAT TACAAAACAT TTCTGGAAAA TGATAGGTTG 
TACATAGTTA TGGAGCTGAT AGAAGGAGCC CCGCTTGGAG AGCATTTCAG TTCTTTGAAG 
GAAAAACATC ACCATTTTAC TGAAGAAAGA CTATGGAAAA TATTTATACA GCTGTGCTTA 
GCTCTTCGAT ACTTACACAA GGAGAAGAGG ATTGTCCATA GAGATCTGAC ACCAAACAAC 
ATTATGTTGG GGGATAAGGA CAAAGTAACC GTTACTGACT TTGGCCTGGC AAAGCAAAAA 
CAAGAAAACA GTAAACTCAC CTCTGTGGTT GGAACAATCC TGTATTCTTG CCCCGAGGTA 
CTGAAGAGTG AGCCGTATGG GGAGAAGGCT GATGTCTGGG CAGTAGGCTG CATCCTTTAT 
CAGATGGCGA CTTTGAGTCC CCCCTTCTAC AGCACTAACA TGCTGTCCTT GGCTACAAAA 
ATAGTGGAGG CGGTATATGA ACCAGTCCCA GAAGGTATCT ACTCTGAAAA AGTAACAGAC 
ACCATCAGCA GGTGCCTCAC TCCTGATGCG GAAGCTCGTC CAGATATTGT AGAAGTCAGT 
TCGATGATAT CAGATGTCAT GATGAAATAT TTAGACAACT TATCTACATC CCAGTTGTCC 
TTGGAAAAGA AGCTAGAACG GGAACGAAGA CGCACACAAA GGTATTTTAT GGAAGCCAAC 
CGGAACACCG TCACATGTCA CCATGAGCTG GCTGTTCTAT CTCACGAGAC CTTTGAGAAG 
GCAAGTTTGA GTAGCAGCAG CAGTGGAGCA GCCAGCCTGA AAAGTGAACT TTCAGAAAGC 
GCAGACCTGC CCCCTGAAGG CTTCCAGGCC TCCTATGGTA AAGACGAAGA CAGGGCCTGT 
GACGAAATCC TGTCAGATGA TAACTTCAAC CTGGAAAATG CTGAGAAAGA TACATATTCA 
GAGGTAGATG ATGAATTGGA CATTTCGGAT AACTCCAGCA GCTCCAGTTC AAGCCCTCTG 
AAAGAATCTA CATTCAACAT TTTAAAGAGA AGTTTTAGTG CTTCAGGAGG AGAAAGACAA 
TCCCAAACAA GGGACTTCAC TGGAGGAACA GGATCAAGAC CAAGACCAGC TTTGCTGCCT 
CTTGACCTGC TTCTGAAAGT GCCACCCCAC ATGCTCAGGG CCCACATTAA GGAAATAGAG 
GCTGAGTTAG TGACAGGGTG GCAGTCCCAT AGCCTTCCTG CTGTGATTCT TCGAAATCTC 
AAAGATCATG GGCCACAGAT GGGCACATTC TTGTGGCAAG CATCAGCAGG AATTGCTGTG 
TCCCAGAGGA AAGTGCGTCA GATCAGTGAT CCTATTCAGC AGATATTAAT TCAGCTGCAC 
AAAATAATCT ATATCACACA GCTTCCTCCA GCTTTGCACC ACAATTTGAA AAGAAGGGTT 
ATAGAGAGAT TCAAGAAATC CCTCTTCAGC CAGCAGAGTA ACCCTTGTAA TTTGAAATCT 
GAAATTAAAA AGTTATCTCA GGGATCTCCA GAACCGATTG AGCCCAACTT TTTCACAGCA 
GATTACCATT TATTACATCG TTCATCCGGT GGAAACAGCC TGTCCCCAAA TGACCCTACA 
GGTTTACCAA CCAGCATTGA ATTGGAGGAA GGAATAACAT ATGAACAGAT GCAGACTGTG 
ATTGAAGAAG TCCTTGAGGA AAGTGGCTAT TACAATTTTA CATCTAACAG GTATCATTCC 
TATCCATGGG GGACCAAGAA TCACCCAACC AAAAGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NEK10 ( XM_017005762.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_016861251.1 Homo sapiens NIMA related kinase 10 (NEK10), transcript variant X3, mRNA.

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CloneID	OHu36991
Clone ID Related Accession (Same CDS sequence)	XM_017005764.1 , XM_017005762.2 , NM_152534.4 , XM_017005761.1 , XM_017005763.1 , XM_017005762.1 , XM_017005761.2 , XM_017005765.1
Accession Version	XM_017005762.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3519bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	serine/threonine-protein kinase Nek10 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000003.12) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_017005762.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCTGATC AAGATAAAAA GGTGAAGACC ACAGAAAAAT CAACTGATAA ACAGCAAGAA 
ATCACCATCA GGGACTATTC AGATCTTAAA AGACTTCGGT GCCTTTTGAA CGTCCAATCA 
AGCAAACAAC AGCTTCCAGC CATTAACTTC GATAGTGCCC AAAATAGCAT GACGAAGTCT 
GAGCCCGCCA TCAGGGCGGG TGGACACAGA GCTCGGGGTC AGTGGCATGA ATCCACAGAA 
GCTGTTGAAC TTGAAAATTT TAGTATAAAC TACAAGAATG AGAGAAATTT CAGCAAACAT 
CCTCAGCGTA AACTATTTCA GGAGATCTTT ACCGCCTTGG TGAAAAATAG ACTCATAAGC 
AGAGAGTGGG TTAATCGAGC CCCATCTATT CATTTTCTGA GAGTGTTAAT CTGTCTGAGG 
CTACTAATGA GGGATCCATG TTATCAGGAA ATACTCCATA GCTTGGGTGG GATTGAAAAC 
CTAGCTCAGT ATATGGAGAT TGTAGCCAAT GAGTACCTCG GCTATGGAGA AGAGCAGCAC 
ACTGTGGACA AGCTGGTCAA CATGACATAT ATTTTTCAAA AACTTGCTGC AGTCAAAGAT 
CAAAGAGAAT GGGTCACCAC AAGTGGAGCC CACAAGACAT TAGTAAATTT ACTTGGTGCC 
CGAGATACTA ATGTTCTATT GGGTTCCCTT CTGGCTCTGG CTAGTTTAGC AGAAAGTCAA 
GAATGTAGGG AGAAGATAAG TGAACTCAAC ATTGTAGAAA ATCTGTTGAT GATTTTACAT 
GAATATGACT TGCTTTCTAA AAGACTAACA GCGGAGTTGC TGCGCCTACT TTGTGCAGAG 
CCCCAGGTGA AAGAGCAGGT GAAGCTCTAT GAGGGGATAC CGGTCCTCCT CAGTCTGCTC 
CACTCTGACC ACTTGAAGCT CCTCTGGAGC ATTGTCTGGA TTCTGGTACA GGTTTGTGAG 
GACCCTGAGA CCAGCGTGGA AATTCGCATT TGGGGAGGCA TCAAACAGCT TCTTCATATT 
TTACAAGGAG ACAGAAATTT TGTTTCTGAT CACTCCTCCA TTGGAAGCCT GTCCAGTGCA 
AATGCTGCAG GCCGAATCCA GCAGCTTCAT TTATCAGAAG ACTTGAGCCC TAGGGAAATA 
CAAGAAAATA CTTTCTCACT TCAAGCAGCC TGCTGTGCTG CCCTCACTGA GCTGGTGCTC 
AATGACACCA ATGCCCACCA GGTGGTTCAG GAAAATGGTG TATATACAAT AGCAAAATTA 
ATTTTACCAA ATAAGCAAAA GAATGCAGCA AAAAGTAATC TATTACAGTG TTATGCTTTC 
AGAGCCTTGA GATTTCTCTT CAGTATGGAA AGAAACAGAC CACTCTTTAA AAGACTTTTC 
CCCACAGACT TGTTTGAGAT CTTCATTGAC ATAGGGCATT ATGTACGTGA TATCAGTGCT 
TATGAAGAAT TGGTATCCAA GCTGAATTTA TTAGTGGAGG ATGAACTGAA GCAAATTGCT 
GAAAATATTG AAAGCATTAA TCAGAACAAA GCTCCTTTGA AATATATAGG CAACTATGCA 
ATTTTGGATC ATCTTGGAAG TGGAGCTTTT GGCTGTGTTT ACAAGGTTAG AAAGCATAGT 
GGTCAAAATC TTTTAGCAAT GAAAGAGGTC AATTTACATA ACCCAGCATT TGGAAAGGAT 
AAGAAAGATC GAGACAGCAG CGTAAGGAAT ATTGTTTCTG AATTAACAAT AATTAAAGAG 
CAGCTTTATC ATCCCAACAT TGTACGTTAT TACAAAACAT TTCTGGAAAA TGATAGGTTG 
TACATAGTTA TGGAGCTGAT AGAAGGAGCC CCGCTTGGAG AGCATTTCAG TTCTTTGAAG 
GAAAAACATC ACCATTTTAC TGAAGAAAGA CTATGGAAAA TATTTATACA GCTGTGCTTA 
GCTCTTCGAT ACTTACACAA GGAGAAGAGG ATTGTCCATA GAGATCTGAC ACCAAACAAC 
ATTATGTTGG GGGATAAGGA CAAAGTAACC GTTACTGACT TTGGCCTGGC AAAGCAAAAA 
CAAGAAAACA GTAAACTCAC CTCTGTGGTT GGAACAATCC TGTATTCTTG CCCCGAGGTA 
CTGAAGAGTG AGCCGTATGG GGAGAAGGCT GATGTCTGGG CAGTAGGCTG CATCCTTTAT 
CAGATGGCGA CTTTGAGTCC CCCCTTCTAC AGCACTAACA TGCTGTCCTT GGCTACAAAA 
ATAGTGGAGG CGGTATATGA ACCAGTCCCA GAAGGTATCT ACTCTGAAAA AGTAACAGAC 
ACCATCAGCA GGTGCCTCAC TCCTGATGCG GAAGCTCGTC CAGATATTGT AGAAGTCAGT 
TCGATGATAT CAGATGTCAT GATGAAATAT TTAGACAACT TATCTACATC CCAGTTGTCC 
TTGGAAAAGA AGCTAGAACG GGAACGAAGA CGCACACAAA GGTATTTTAT GGAAGCCAAC 
CGGAACACCG TCACATGTCA CCATGAGCTG GCTGTTCTAT CTCACGAGAC CTTTGAGAAG 
GCAAGTTTGA GTAGCAGCAG CAGTGGAGCA GCCAGCCTGA AAAGTGAACT TTCAGAAAGC 
GCAGACCTGC CCCCTGAAGG CTTCCAGGCC TCCTATGGTA AAGACGAAGA CAGGGCCTGT 
GACGAAATCC TGTCAGATGA TAACTTCAAC CTGGAAAATG CTGAGAAAGA TACATATTCA 
GAGGTAGATG ATGAATTGGA CATTTCGGAT AACTCCAGCA GCTCCAGTTC AAGCCCTCTG 
AAAGAATCTA CATTCAACAT TTTAAAGAGA AGTTTTAGTG CTTCAGGAGG AGAAAGACAA 
TCCCAAACAA GGGACTTCAC TGGAGGAACA GGATCAAGAC CAAGACCAGC TTTGCTGCCT 
CTTGACCTGC TTCTGAAAGT GCCACCCCAC ATGCTCAGGG CCCACATTAA GGAAATAGAG 
GCTGAGTTAG TGACAGGGTG GCAGTCCCAT AGCCTTCCTG CTGTGATTCT TCGAAATCTC 
AAAGATCATG GGCCACAGAT GGGCACATTC TTGTGGCAAG CATCAGCAGG AATTGCTGTG 
TCCCAGAGGA AAGTGCGTCA GATCAGTGAT CCTATTCAGC AGATATTAAT TCAGCTGCAC 
AAAATAATCT ATATCACACA GCTTCCTCCA GCTTTGCACC ACAATTTGAA AAGAAGGGTT 
ATAGAGAGAT TCAAGAAATC CCTCTTCAGC CAGCAGAGTA ACCCTTGTAA TTTGAAATCT 
GAAATTAAAA AGTTATCTCA GGGATCTCCA GAACCGATTG AGCCCAACTT TTTCACAGCA 
GATTACCATT TATTACATCG TTCATCCGGT GGAAACAGCC TGTCCCCAAA TGACCCTACA 
GGTTTACCAA CCAGCATTGA ATTGGAGGAA GGAATAACAT ATGAACAGAT GCAGACTGTG 
ATTGAAGAAG TCCTTGAGGA AAGTGGCTAT TACAATTTTA CATCTAACAG GTATCATTCC 
TATCCATGGG GGACCAAGAA TCACCCAACC AAAAGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016861251.1
CDS	387..3905
Translation	MPDQDKKVKTTEKSTDKQQEITIRDYSDLKRLRCLLNVQSSKQQLPAINFDSAQNSMTKSEPAIRAGGHRARGQWHESTEAVELENFSINYKNERNFSKHPQRKLFQEIFTALVKNRLISREWVNRAPSIHFLRVLICLRLLMRDPCYQEILHSLGGIENLAQYMEIVANEYLGYGEEQHTVDKLVNMTYIFQKLAAVKDQREWVTTSGAHKTLVNLLGARDTNVLLGSLLALASLAESQECREKISELNIVENLLMILHEYDLLSKRLTAELLRLLCAEPQVKEQVKLYEGIPVLLSLLHSDHLKLLWSIVWILVQVCEDPETSVEIRIWGGIKQLLHILQGDRNFVSDHSSIGSLSSANAAGRIQQLHLSEDLSPREIQENTFSLQAACCAALTELVLNDTNAHQVVQENGVYTIAKLILPNKQKNAAKSNLLQCYAFRALRFLFSMERNRPLFKRLFPTDLFEIFIDIGHYVRDISAYEELVSKLNLLVEDELKQIAENIESINQNKAPLKYIGNYAILDHLGSGAFGCVYKVRKHSGQNLLAMKEVNLHNPAFGKDKKDRDSSVRNIVSELTIIKEQLYHPNIVRYYKTFLENDRLYIVMELIEGAPLGEHFSSLKEKHHHFTEERLWKIFIQLCLALRYLHKEKRIVHRDLTPNNIMLGDKDKVTVTDFGLAKQKQENSKLTSVVGTILYSCPEVLKSEPYGEKADVWAVGCILYQMATLSPPFYSTNMLSLATKIVEAVYEPVPEGIYSEKVTDTISRCLTPDAEARPDIVEVSSMISDVMMKYLDNLSTSQLSLEKKLERERRRTQRYFMEANRNTVTCHHELAVLSHETFEKASLSSSSSGAASLKSELSESADLPPEGFQASYGKDEDRACDEILSDDNFNLENAEKDTYSEVDDELDISDNSSSSSSSPLKESTFNILKRSFSASGGERQSQTRDFTGGTGSRPRPALLPLDLLLKVPPHMLRAHIKEIEAELVTGWQSHSLPAVILRNLKDHGPQMGTFLWQASAGIAVSQRKVRQISDPIQQILIQLHKIIYITQLPPALHHNLKRRVIERFKKSLFSQQSNPCNLKSEIKKLSQGSPEPIEPNFFTADYHLLHRSSGGNSLSPNDPTGLPTSIELEEGITYEQMQTVIEEVLEESGYYNFTSNRYHSYPWGTKNHPTKR

Target ORF information:

RefSeq Version	XM_017005762.2
Organism	Homo sapiens(human)
Definition	Homo sapiens NIMA related kinase 10 (NEK10), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017005762.2

ORF Insert Sequence:

ATGCCTGATC AAGATAAAAA GGTGAAGACC ACAGAAAAAT CAACTGATAA ACAGCAAGAA 
ATCACCATCA GGGACTATTC AGATCTTAAA AGACTTCGGT GCCTTTTGAA CGTCCAATCA 
AGCAAACAAC AGCTTCCAGC CATTAACTTC GATAGTGCCC AAAATAGCAT GACGAAGTCT 
GAGCCCGCCA TCAGGGCGGG TGGACACAGA GCTCGGGGTC AGTGGCATGA ATCCACAGAA 
GCTGTTGAAC TTGAAAATTT TAGTATAAAC TACAAGAATG AGAGAAATTT CAGCAAACAT 
CCTCAGCGTA AACTATTTCA GGAGATCTTT ACCGCCTTGG TGAAAAATAG ACTCATAAGC 
AGAGAGTGGG TTAATCGAGC CCCATCTATT CATTTTCTGA GAGTGTTAAT CTGTCTGAGG 
CTACTAATGA GGGATCCATG TTATCAGGAA ATACTCCATA GCTTGGGTGG GATTGAAAAC 
CTAGCTCAGT ATATGGAGAT TGTAGCCAAT GAGTACCTCG GCTATGGAGA AGAGCAGCAC 
ACTGTGGACA AGCTGGTCAA CATGACATAT ATTTTTCAAA AACTTGCTGC AGTCAAAGAT 
CAAAGAGAAT GGGTCACCAC AAGTGGAGCC CACAAGACAT TAGTAAATTT ACTTGGTGCC 
CGAGATACTA ATGTTCTATT GGGTTCCCTT CTGGCTCTGG CTAGTTTAGC AGAAAGTCAA 
GAATGTAGGG AGAAGATAAG TGAACTCAAC ATTGTAGAAA ATCTGTTGAT GATTTTACAT 
GAATATGACT TGCTTTCTAA AAGACTAACA GCGGAGTTGC TGCGCCTACT TTGTGCAGAG 
CCCCAGGTGA AAGAGCAGGT GAAGCTCTAT GAGGGGATAC CGGTCCTCCT CAGTCTGCTC 
CACTCTGACC ACTTGAAGCT CCTCTGGAGC ATTGTCTGGA TTCTGGTACA GGTTTGTGAG 
GACCCTGAGA CCAGCGTGGA AATTCGCATT TGGGGAGGCA TCAAACAGCT TCTTCATATT 
TTACAAGGAG ACAGAAATTT TGTTTCTGAT CACTCCTCCA TTGGAAGCCT GTCCAGTGCA 
AATGCTGCAG GCCGAATCCA GCAGCTTCAT TTATCAGAAG ACTTGAGCCC TAGGGAAATA 
CAAGAAAATA CTTTCTCACT TCAAGCAGCC TGCTGTGCTG CCCTCACTGA GCTGGTGCTC 
AATGACACCA ATGCCCACCA GGTGGTTCAG GAAAATGGTG TATATACAAT AGCAAAATTA 
ATTTTACCAA ATAAGCAAAA GAATGCAGCA AAAAGTAATC TATTACAGTG TTATGCTTTC 
AGAGCCTTGA GATTTCTCTT CAGTATGGAA AGAAACAGAC CACTCTTTAA AAGACTTTTC 
CCCACAGACT TGTTTGAGAT CTTCATTGAC ATAGGGCATT ATGTACGTGA TATCAGTGCT 
TATGAAGAAT TGGTATCCAA GCTGAATTTA TTAGTGGAGG ATGAACTGAA GCAAATTGCT 
GAAAATATTG AAAGCATTAA TCAGAACAAA GCTCCTTTGA AATATATAGG CAACTATGCA 
ATTTTGGATC ATCTTGGAAG TGGAGCTTTT GGCTGTGTTT ACAAGGTTAG AAAGCATAGT 
GGTCAAAATC TTTTAGCAAT GAAAGAGGTC AATTTACATA ACCCAGCATT TGGAAAGGAT 
AAGAAAGATC GAGACAGCAG CGTAAGGAAT ATTGTTTCTG AATTAACAAT AATTAAAGAG 
CAGCTTTATC ATCCCAACAT TGTACGTTAT TACAAAACAT TTCTGGAAAA TGATAGGTTG 
TACATAGTTA TGGAGCTGAT AGAAGGAGCC CCGCTTGGAG AGCATTTCAG TTCTTTGAAG 
GAAAAACATC ACCATTTTAC TGAAGAAAGA CTATGGAAAA TATTTATACA GCTGTGCTTA 
GCTCTTCGAT ACTTACACAA GGAGAAGAGG ATTGTCCATA GAGATCTGAC ACCAAACAAC 
ATTATGTTGG GGGATAAGGA CAAAGTAACC GTTACTGACT TTGGCCTGGC AAAGCAAAAA 
CAAGAAAACA GTAAACTCAC CTCTGTGGTT GGAACAATCC TGTATTCTTG CCCCGAGGTA 
CTGAAGAGTG AGCCGTATGG GGAGAAGGCT GATGTCTGGG CAGTAGGCTG CATCCTTTAT 
CAGATGGCGA CTTTGAGTCC CCCCTTCTAC AGCACTAACA TGCTGTCCTT GGCTACAAAA 
ATAGTGGAGG CGGTATATGA ACCAGTCCCA GAAGGTATCT ACTCTGAAAA AGTAACAGAC 
ACCATCAGCA GGTGCCTCAC TCCTGATGCG GAAGCTCGTC CAGATATTGT AGAAGTCAGT 
TCGATGATAT CAGATGTCAT GATGAAATAT TTAGACAACT TATCTACATC CCAGTTGTCC 
TTGGAAAAGA AGCTAGAACG GGAACGAAGA CGCACACAAA GGTATTTTAT GGAAGCCAAC 
CGGAACACCG TCACATGTCA CCATGAGCTG GCTGTTCTAT CTCACGAGAC CTTTGAGAAG 
GCAAGTTTGA GTAGCAGCAG CAGTGGAGCA GCCAGCCTGA AAAGTGAACT TTCAGAAAGC 
GCAGACCTGC CCCCTGAAGG CTTCCAGGCC TCCTATGGTA AAGACGAAGA CAGGGCCTGT 
GACGAAATCC TGTCAGATGA TAACTTCAAC CTGGAAAATG CTGAGAAAGA TACATATTCA 
GAGGTAGATG ATGAATTGGA CATTTCGGAT AACTCCAGCA GCTCCAGTTC AAGCCCTCTG 
AAAGAATCTA CATTCAACAT TTTAAAGAGA AGTTTTAGTG CTTCAGGAGG AGAAAGACAA 
TCCCAAACAA GGGACTTCAC TGGAGGAACA GGATCAAGAC CAAGACCAGC TTTGCTGCCT 
CTTGACCTGC TTCTGAAAGT GCCACCCCAC ATGCTCAGGG CCCACATTAA GGAAATAGAG 
GCTGAGTTAG TGACAGGGTG GCAGTCCCAT AGCCTTCCTG CTGTGATTCT TCGAAATCTC 
AAAGATCATG GGCCACAGAT GGGCACATTC TTGTGGCAAG CATCAGCAGG AATTGCTGTG 
TCCCAGAGGA AAGTGCGTCA GATCAGTGAT CCTATTCAGC AGATATTAAT TCAGCTGCAC 
AAAATAATCT ATATCACACA GCTTCCTCCA GCTTTGCACC ACAATTTGAA AAGAAGGGTT 
ATAGAGAGAT TCAAGAAATC CCTCTTCAGC CAGCAGAGTA ACCCTTGTAA TTTGAAATCT 
GAAATTAAAA AGTTATCTCA GGGATCTCCA GAACCGATTG AGCCCAACTT TTTCACAGCA 
GATTACCATT TATTACATCG TTCATCCGGT GGAAACAGCC TGTCCCCAAA TGACCCTACA 
GGTTTACCAA CCAGCATTGA ATTGGAGGAA GGAATAACAT ATGAACAGAT GCAGACTGTG 
ATTGAAGAAG TCCTTGAGGA AAGTGGCTAT TACAATTTTA CATCTAACAG GTATCATTCC 
TATCCATGGG GGACCAAGAA TCACCCAACC AAAAGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NEK10 ( XM_017005761.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016861250.1 Homo sapiens NIMA related kinase 10 (NEK10), transcript variant X2, mRNA.

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CloneID	OHu36991
Clone ID Related Accession (Same CDS sequence)	XM_017005764.1 , XM_017005762.2 , NM_152534.4 , XM_017005761.1 , XM_017005763.1 , XM_017005762.1 , XM_017005761.2 , XM_017005765.1
Accession Version	XM_017005761.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3519bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	serine/threonine-protein kinase Nek10 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000003.12) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCTGATC AAGATAAAAA GGTGAAGACC ACAGAAAAAT CAACTGATAA ACAGCAAGAA 
ATCACCATCA GGGACTATTC AGATCTTAAA AGACTTCGGT GCCTTTTGAA CGTCCAATCA 
AGCAAACAAC AGCTTCCAGC CATTAACTTC GATAGTGCCC AAAATAGCAT GACGAAGTCT 
GAGCCCGCCA TCAGGGCGGG TGGACACAGA GCTCGGGGTC AGTGGCATGA ATCCACAGAA 
GCTGTTGAAC TTGAAAATTT TAGTATAAAC TACAAGAATG AGAGAAATTT CAGCAAACAT 
CCTCAGCGTA AACTATTTCA GGAGATCTTT ACCGCCTTGG TGAAAAATAG ACTCATAAGC 
AGAGAGTGGG TTAATCGAGC CCCATCTATT CATTTTCTGA GAGTGTTAAT CTGTCTGAGG 
CTACTAATGA GGGATCCATG TTATCAGGAA ATACTCCATA GCTTGGGTGG GATTGAAAAC 
CTAGCTCAGT ATATGGAGAT TGTAGCCAAT GAGTACCTCG GCTATGGAGA AGAGCAGCAC 
ACTGTGGACA AGCTGGTCAA CATGACATAT ATTTTTCAAA AACTTGCTGC AGTCAAAGAT 
CAAAGAGAAT GGGTCACCAC AAGTGGAGCC CACAAGACAT TAGTAAATTT ACTTGGTGCC 
CGAGATACTA ATGTTCTATT GGGTTCCCTT CTGGCTCTGG CTAGTTTAGC AGAAAGTCAA 
GAATGTAGGG AGAAGATAAG TGAACTCAAC ATTGTAGAAA ATCTGTTGAT GATTTTACAT 
GAATATGACT TGCTTTCTAA AAGACTAACA GCGGAGTTGC TGCGCCTACT TTGTGCAGAG 
CCCCAGGTGA AAGAGCAGGT GAAGCTCTAT GAGGGGATAC CGGTCCTCCT CAGTCTGCTC 
CACTCTGACC ACTTGAAGCT CCTCTGGAGC ATTGTCTGGA TTCTGGTACA GGTTTGTGAG 
GACCCTGAGA CCAGCGTGGA AATTCGCATT TGGGGAGGCA TCAAACAGCT TCTTCATATT 
TTACAAGGAG ACAGAAATTT TGTTTCTGAT CACTCCTCCA TTGGAAGCCT GTCCAGTGCA 
AATGCTGCAG GCCGAATCCA GCAGCTTCAT TTATCAGAAG ACTTGAGCCC TAGGGAAATA 
CAAGAAAATA CTTTCTCACT TCAAGCAGCC TGCTGTGCTG CCCTCACTGA GCTGGTGCTC 
AATGACACCA ATGCCCACCA GGTGGTTCAG GAAAATGGTG TATATACAAT AGCAAAATTA 
ATTTTACCAA ATAAGCAAAA GAATGCAGCA AAAAGTAATC TATTACAGTG TTATGCTTTC 
AGAGCCTTGA GATTTCTCTT CAGTATGGAA AGAAACAGAC CACTCTTTAA AAGACTTTTC 
CCCACAGACT TGTTTGAGAT CTTCATTGAC ATAGGGCATT ATGTACGTGA TATCAGTGCT 
TATGAAGAAT TGGTATCCAA GCTGAATTTA TTAGTGGAGG ATGAACTGAA GCAAATTGCT 
GAAAATATTG AAAGCATTAA TCAGAACAAA GCTCCTTTGA AATATATAGG CAACTATGCA 
ATTTTGGATC ATCTTGGAAG TGGAGCTTTT GGCTGTGTTT ACAAGGTTAG AAAGCATAGT 
GGTCAAAATC TTTTAGCAAT GAAAGAGGTC AATTTACATA ACCCAGCATT TGGAAAGGAT 
AAGAAAGATC GAGACAGCAG CGTAAGGAAT ATTGTTTCTG AATTAACAAT AATTAAAGAG 
CAGCTTTATC ATCCCAACAT TGTACGTTAT TACAAAACAT TTCTGGAAAA TGATAGGTTG 
TACATAGTTA TGGAGCTGAT AGAAGGAGCC CCGCTTGGAG AGCATTTCAG TTCTTTGAAG 
GAAAAACATC ACCATTTTAC TGAAGAAAGA CTATGGAAAA TATTTATACA GCTGTGCTTA 
GCTCTTCGAT ACTTACACAA GGAGAAGAGG ATTGTCCATA GAGATCTGAC ACCAAACAAC 
ATTATGTTGG GGGATAAGGA CAAAGTAACC GTTACTGACT TTGGCCTGGC AAAGCAAAAA 
CAAGAAAACA GTAAACTCAC CTCTGTGGTT GGAACAATCC TGTATTCTTG CCCCGAGGTA 
CTGAAGAGTG AGCCGTATGG GGAGAAGGCT GATGTCTGGG CAGTAGGCTG CATCCTTTAT 
CAGATGGCGA CTTTGAGTCC CCCCTTCTAC AGCACTAACA TGCTGTCCTT GGCTACAAAA 
ATAGTGGAGG CGGTATATGA ACCAGTCCCA GAAGGTATCT ACTCTGAAAA AGTAACAGAC 
ACCATCAGCA GGTGCCTCAC TCCTGATGCG GAAGCTCGTC CAGATATTGT AGAAGTCAGT 
TCGATGATAT CAGATGTCAT GATGAAATAT TTAGACAACT TATCTACATC CCAGTTGTCC 
TTGGAAAAGA AGCTAGAACG GGAACGAAGA CGCACACAAA GGTATTTTAT GGAAGCCAAC 
CGGAACACCG TCACATGTCA CCATGAGCTG GCTGTTCTAT CTCACGAGAC CTTTGAGAAG 
GCAAGTTTGA GTAGCAGCAG CAGTGGAGCA GCCAGCCTGA AAAGTGAACT TTCAGAAAGC 
GCAGACCTGC CCCCTGAAGG CTTCCAGGCC TCCTATGGTA AAGACGAAGA CAGGGCCTGT 
GACGAAATCC TGTCAGATGA TAACTTCAAC CTGGAAAATG CTGAGAAAGA TACATATTCA 
GAGGTAGATG ATGAATTGGA CATTTCGGAT AACTCCAGCA GCTCCAGTTC AAGCCCTCTG 
AAAGAATCTA CATTCAACAT TTTAAAGAGA AGTTTTAGTG CTTCAGGAGG AGAAAGACAA 
TCCCAAACAA GGGACTTCAC TGGAGGAACA GGATCAAGAC CAAGACCAGC TTTGCTGCCT 
CTTGACCTGC TTCTGAAAGT GCCACCCCAC ATGCTCAGGG CCCACATTAA GGAAATAGAG 
GCTGAGTTAG TGACAGGGTG GCAGTCCCAT AGCCTTCCTG CTGTGATTCT TCGAAATCTC 
AAAGATCATG GGCCACAGAT GGGCACATTC TTGTGGCAAG CATCAGCAGG AATTGCTGTG 
TCCCAGAGGA AAGTGCGTCA GATCAGTGAT CCTATTCAGC AGATATTAAT TCAGCTGCAC 
AAAATAATCT ATATCACACA GCTTCCTCCA GCTTTGCACC ACAATTTGAA AAGAAGGGTT 
ATAGAGAGAT TCAAGAAATC CCTCTTCAGC CAGCAGAGTA ACCCTTGTAA TTTGAAATCT 
GAAATTAAAA AGTTATCTCA GGGATCTCCA GAACCGATTG AGCCCAACTT TTTCACAGCA 
GATTACCATT TATTACATCG TTCATCCGGT GGAAACAGCC TGTCCCCAAA TGACCCTACA 
GGTTTACCAA CCAGCATTGA ATTGGAGGAA GGAATAACAT ATGAACAGAT GCAGACTGTG 
ATTGAAGAAG TCCTTGAGGA AAGTGGCTAT TACAATTTTA CATCTAACAG GTATCATTCC 
TATCCATGGG GGACCAAGAA TCACCCAACC AAAAGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016861250.1
CDS	227..3745
Translation	MPDQDKKVKTTEKSTDKQQEITIRDYSDLKRLRCLLNVQSSKQQLPAINFDSAQNSMTKSEPAIRAGGHRARGQWHESTEAVELENFSINYKNERNFSKHPQRKLFQEIFTALVKNRLISREWVNRAPSIHFLRVLICLRLLMRDPCYQEILHSLGGIENLAQYMEIVANEYLGYGEEQHTVDKLVNMTYIFQKLAAVKDQREWVTTSGAHKTLVNLLGARDTNVLLGSLLALASLAESQECREKISELNIVENLLMILHEYDLLSKRLTAELLRLLCAEPQVKEQVKLYEGIPVLLSLLHSDHLKLLWSIVWILVQVCEDPETSVEIRIWGGIKQLLHILQGDRNFVSDHSSIGSLSSANAAGRIQQLHLSEDLSPREIQENTFSLQAACCAALTELVLNDTNAHQVVQENGVYTIAKLILPNKQKNAAKSNLLQCYAFRALRFLFSMERNRPLFKRLFPTDLFEIFIDIGHYVRDISAYEELVSKLNLLVEDELKQIAENIESINQNKAPLKYIGNYAILDHLGSGAFGCVYKVRKHSGQNLLAMKEVNLHNPAFGKDKKDRDSSVRNIVSELTIIKEQLYHPNIVRYYKTFLENDRLYIVMELIEGAPLGEHFSSLKEKHHHFTEERLWKIFIQLCLALRYLHKEKRIVHRDLTPNNIMLGDKDKVTVTDFGLAKQKQENSKLTSVVGTILYSCPEVLKSEPYGEKADVWAVGCILYQMATLSPPFYSTNMLSLATKIVEAVYEPVPEGIYSEKVTDTISRCLTPDAEARPDIVEVSSMISDVMMKYLDNLSTSQLSLEKKLERERRRTQRYFMEANRNTVTCHHELAVLSHETFEKASLSSSSSGAASLKSELSESADLPPEGFQASYGKDEDRACDEILSDDNFNLENAEKDTYSEVDDELDISDNSSSSSSSPLKESTFNILKRSFSASGGERQSQTRDFTGGTGSRPRPALLPLDLLLKVPPHMLRAHIKEIEAELVTGWQSHSLPAVILRNLKDHGPQMGTFLWQASAGIAVSQRKVRQISDPIQQILIQLHKIIYITQLPPALHHNLKRRVIERFKKSLFSQQSNPCNLKSEIKKLSQGSPEPIEPNFFTADYHLLHRSSGGNSLSPNDPTGLPTSIELEEGITYEQMQTVIEEVLEESGYYNFTSNRYHSYPWGTKNHPTKR

Target ORF information:

RefSeq Version	XM_017005761.1
Organism	Homo sapiens(human)
Definition	Homo sapiens NIMA related kinase 10 (NEK10), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017005761.1

ORF Insert Sequence:

ATGCCTGATC AAGATAAAAA GGTGAAGACC ACAGAAAAAT CAACTGATAA ACAGCAAGAA 
ATCACCATCA GGGACTATTC AGATCTTAAA AGACTTCGGT GCCTTTTGAA CGTCCAATCA 
AGCAAACAAC AGCTTCCAGC CATTAACTTC GATAGTGCCC AAAATAGCAT GACGAAGTCT 
GAGCCCGCCA TCAGGGCGGG TGGACACAGA GCTCGGGGTC AGTGGCATGA ATCCACAGAA 
GCTGTTGAAC TTGAAAATTT TAGTATAAAC TACAAGAATG AGAGAAATTT CAGCAAACAT 
CCTCAGCGTA AACTATTTCA GGAGATCTTT ACCGCCTTGG TGAAAAATAG ACTCATAAGC 
AGAGAGTGGG TTAATCGAGC CCCATCTATT CATTTTCTGA GAGTGTTAAT CTGTCTGAGG 
CTACTAATGA GGGATCCATG TTATCAGGAA ATACTCCATA GCTTGGGTGG GATTGAAAAC 
CTAGCTCAGT ATATGGAGAT TGTAGCCAAT GAGTACCTCG GCTATGGAGA AGAGCAGCAC 
ACTGTGGACA AGCTGGTCAA CATGACATAT ATTTTTCAAA AACTTGCTGC AGTCAAAGAT 
CAAAGAGAAT GGGTCACCAC AAGTGGAGCC CACAAGACAT TAGTAAATTT ACTTGGTGCC 
CGAGATACTA ATGTTCTATT GGGTTCCCTT CTGGCTCTGG CTAGTTTAGC AGAAAGTCAA 
GAATGTAGGG AGAAGATAAG TGAACTCAAC ATTGTAGAAA ATCTGTTGAT GATTTTACAT 
GAATATGACT TGCTTTCTAA AAGACTAACA GCGGAGTTGC TGCGCCTACT TTGTGCAGAG 
CCCCAGGTGA AAGAGCAGGT GAAGCTCTAT GAGGGGATAC CGGTCCTCCT CAGTCTGCTC 
CACTCTGACC ACTTGAAGCT CCTCTGGAGC ATTGTCTGGA TTCTGGTACA GGTTTGTGAG 
GACCCTGAGA CCAGCGTGGA AATTCGCATT TGGGGAGGCA TCAAACAGCT TCTTCATATT 
TTACAAGGAG ACAGAAATTT TGTTTCTGAT CACTCCTCCA TTGGAAGCCT GTCCAGTGCA 
AATGCTGCAG GCCGAATCCA GCAGCTTCAT TTATCAGAAG ACTTGAGCCC TAGGGAAATA 
CAAGAAAATA CTTTCTCACT TCAAGCAGCC TGCTGTGCTG CCCTCACTGA GCTGGTGCTC 
AATGACACCA ATGCCCACCA GGTGGTTCAG GAAAATGGTG TATATACAAT AGCAAAATTA 
ATTTTACCAA ATAAGCAAAA GAATGCAGCA AAAAGTAATC TATTACAGTG TTATGCTTTC 
AGAGCCTTGA GATTTCTCTT CAGTATGGAA AGAAACAGAC CACTCTTTAA AAGACTTTTC 
CCCACAGACT TGTTTGAGAT CTTCATTGAC ATAGGGCATT ATGTACGTGA TATCAGTGCT 
TATGAAGAAT TGGTATCCAA GCTGAATTTA TTAGTGGAGG ATGAACTGAA GCAAATTGCT 
GAAAATATTG AAAGCATTAA TCAGAACAAA GCTCCTTTGA AATATATAGG CAACTATGCA 
ATTTTGGATC ATCTTGGAAG TGGAGCTTTT GGCTGTGTTT ACAAGGTTAG AAAGCATAGT 
GGTCAAAATC TTTTAGCAAT GAAAGAGGTC AATTTACATA ACCCAGCATT TGGAAAGGAT 
AAGAAAGATC GAGACAGCAG CGTAAGGAAT ATTGTTTCTG AATTAACAAT AATTAAAGAG 
CAGCTTTATC ATCCCAACAT TGTACGTTAT TACAAAACAT TTCTGGAAAA TGATAGGTTG 
TACATAGTTA TGGAGCTGAT AGAAGGAGCC CCGCTTGGAG AGCATTTCAG TTCTTTGAAG 
GAAAAACATC ACCATTTTAC TGAAGAAAGA CTATGGAAAA TATTTATACA GCTGTGCTTA 
GCTCTTCGAT ACTTACACAA GGAGAAGAGG ATTGTCCATA GAGATCTGAC ACCAAACAAC 
ATTATGTTGG GGGATAAGGA CAAAGTAACC GTTACTGACT TTGGCCTGGC AAAGCAAAAA 
CAAGAAAACA GTAAACTCAC CTCTGTGGTT GGAACAATCC TGTATTCTTG CCCCGAGGTA 
CTGAAGAGTG AGCCGTATGG GGAGAAGGCT GATGTCTGGG CAGTAGGCTG CATCCTTTAT 
CAGATGGCGA CTTTGAGTCC CCCCTTCTAC AGCACTAACA TGCTGTCCTT GGCTACAAAA 
ATAGTGGAGG CGGTATATGA ACCAGTCCCA GAAGGTATCT ACTCTGAAAA AGTAACAGAC 
ACCATCAGCA GGTGCCTCAC TCCTGATGCG GAAGCTCGTC CAGATATTGT AGAAGTCAGT 
TCGATGATAT CAGATGTCAT GATGAAATAT TTAGACAACT TATCTACATC CCAGTTGTCC 
TTGGAAAAGA AGCTAGAACG GGAACGAAGA CGCACACAAA GGTATTTTAT GGAAGCCAAC 
CGGAACACCG TCACATGTCA CCATGAGCTG GCTGTTCTAT CTCACGAGAC CTTTGAGAAG 
GCAAGTTTGA GTAGCAGCAG CAGTGGAGCA GCCAGCCTGA AAAGTGAACT TTCAGAAAGC 
GCAGACCTGC CCCCTGAAGG CTTCCAGGCC TCCTATGGTA AAGACGAAGA CAGGGCCTGT 
GACGAAATCC TGTCAGATGA TAACTTCAAC CTGGAAAATG CTGAGAAAGA TACATATTCA 
GAGGTAGATG ATGAATTGGA CATTTCGGAT AACTCCAGCA GCTCCAGTTC AAGCCCTCTG 
AAAGAATCTA CATTCAACAT TTTAAAGAGA AGTTTTAGTG CTTCAGGAGG AGAAAGACAA 
TCCCAAACAA GGGACTTCAC TGGAGGAACA GGATCAAGAC CAAGACCAGC TTTGCTGCCT 
CTTGACCTGC TTCTGAAAGT GCCACCCCAC ATGCTCAGGG CCCACATTAA GGAAATAGAG 
GCTGAGTTAG TGACAGGGTG GCAGTCCCAT AGCCTTCCTG CTGTGATTCT TCGAAATCTC 
AAAGATCATG GGCCACAGAT GGGCACATTC TTGTGGCAAG CATCAGCAGG AATTGCTGTG 
TCCCAGAGGA AAGTGCGTCA GATCAGTGAT CCTATTCAGC AGATATTAAT TCAGCTGCAC 
AAAATAATCT ATATCACACA GCTTCCTCCA GCTTTGCACC ACAATTTGAA AAGAAGGGTT 
ATAGAGAGAT TCAAGAAATC CCTCTTCAGC CAGCAGAGTA ACCCTTGTAA TTTGAAATCT 
GAAATTAAAA AGTTATCTCA GGGATCTCCA GAACCGATTG AGCCCAACTT TTTCACAGCA 
GATTACCATT TATTACATCG TTCATCCGGT GGAAACAGCC TGTCCCCAAA TGACCCTACA 
GGTTTACCAA CCAGCATTGA ATTGGAGGAA GGAATAACAT ATGAACAGAT GCAGACTGTG 
ATTGAAGAAG TCCTTGAGGA AAGTGGCTAT TACAATTTTA CATCTAACAG GTATCATTCC 
TATCCATGGG GGACCAAGAA TCACCCAACC AAAAGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NEK10 ( XM_017005763.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016861252.1 Homo sapiens NIMA related kinase 10 (NEK10), transcript variant X4, mRNA.

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CloneID	OHu36991
Clone ID Related Accession (Same CDS sequence)	XM_017005764.1 , XM_017005762.2 , NM_152534.4 , XM_017005761.1 , XM_017005763.1 , XM_017005762.1 , XM_017005761.2 , XM_017005765.1
Accession Version	XM_017005763.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3519bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	serine/threonine-protein kinase Nek10 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000003.12) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCTGATC AAGATAAAAA GGTGAAGACC ACAGAAAAAT CAACTGATAA ACAGCAAGAA 
ATCACCATCA GGGACTATTC AGATCTTAAA AGACTTCGGT GCCTTTTGAA CGTCCAATCA 
AGCAAACAAC AGCTTCCAGC CATTAACTTC GATAGTGCCC AAAATAGCAT GACGAAGTCT 
GAGCCCGCCA TCAGGGCGGG TGGACACAGA GCTCGGGGTC AGTGGCATGA ATCCACAGAA 
GCTGTTGAAC TTGAAAATTT TAGTATAAAC TACAAGAATG AGAGAAATTT CAGCAAACAT 
CCTCAGCGTA AACTATTTCA GGAGATCTTT ACCGCCTTGG TGAAAAATAG ACTCATAAGC 
AGAGAGTGGG TTAATCGAGC CCCATCTATT CATTTTCTGA GAGTGTTAAT CTGTCTGAGG 
CTACTAATGA GGGATCCATG TTATCAGGAA ATACTCCATA GCTTGGGTGG GATTGAAAAC 
CTAGCTCAGT ATATGGAGAT TGTAGCCAAT GAGTACCTCG GCTATGGAGA AGAGCAGCAC 
ACTGTGGACA AGCTGGTCAA CATGACATAT ATTTTTCAAA AACTTGCTGC AGTCAAAGAT 
CAAAGAGAAT GGGTCACCAC AAGTGGAGCC CACAAGACAT TAGTAAATTT ACTTGGTGCC 
CGAGATACTA ATGTTCTATT GGGTTCCCTT CTGGCTCTGG CTAGTTTAGC AGAAAGTCAA 
GAATGTAGGG AGAAGATAAG TGAACTCAAC ATTGTAGAAA ATCTGTTGAT GATTTTACAT 
GAATATGACT TGCTTTCTAA AAGACTAACA GCGGAGTTGC TGCGCCTACT TTGTGCAGAG 
CCCCAGGTGA AAGAGCAGGT GAAGCTCTAT GAGGGGATAC CGGTCCTCCT CAGTCTGCTC 
CACTCTGACC ACTTGAAGCT CCTCTGGAGC ATTGTCTGGA TTCTGGTACA GGTTTGTGAG 
GACCCTGAGA CCAGCGTGGA AATTCGCATT TGGGGAGGCA TCAAACAGCT TCTTCATATT 
TTACAAGGAG ACAGAAATTT TGTTTCTGAT CACTCCTCCA TTGGAAGCCT GTCCAGTGCA 
AATGCTGCAG GCCGAATCCA GCAGCTTCAT TTATCAGAAG ACTTGAGCCC TAGGGAAATA 
CAAGAAAATA CTTTCTCACT TCAAGCAGCC TGCTGTGCTG CCCTCACTGA GCTGGTGCTC 
AATGACACCA ATGCCCACCA GGTGGTTCAG GAAAATGGTG TATATACAAT AGCAAAATTA 
ATTTTACCAA ATAAGCAAAA GAATGCAGCA AAAAGTAATC TATTACAGTG TTATGCTTTC 
AGAGCCTTGA GATTTCTCTT CAGTATGGAA AGAAACAGAC CACTCTTTAA AAGACTTTTC 
CCCACAGACT TGTTTGAGAT CTTCATTGAC ATAGGGCATT ATGTACGTGA TATCAGTGCT 
TATGAAGAAT TGGTATCCAA GCTGAATTTA TTAGTGGAGG ATGAACTGAA GCAAATTGCT 
GAAAATATTG AAAGCATTAA TCAGAACAAA GCTCCTTTGA AATATATAGG CAACTATGCA 
ATTTTGGATC ATCTTGGAAG TGGAGCTTTT GGCTGTGTTT ACAAGGTTAG AAAGCATAGT 
GGTCAAAATC TTTTAGCAAT GAAAGAGGTC AATTTACATA ACCCAGCATT TGGAAAGGAT 
AAGAAAGATC GAGACAGCAG CGTAAGGAAT ATTGTTTCTG AATTAACAAT AATTAAAGAG 
CAGCTTTATC ATCCCAACAT TGTACGTTAT TACAAAACAT TTCTGGAAAA TGATAGGTTG 
TACATAGTTA TGGAGCTGAT AGAAGGAGCC CCGCTTGGAG AGCATTTCAG TTCTTTGAAG 
GAAAAACATC ACCATTTTAC TGAAGAAAGA CTATGGAAAA TATTTATACA GCTGTGCTTA 
GCTCTTCGAT ACTTACACAA GGAGAAGAGG ATTGTCCATA GAGATCTGAC ACCAAACAAC 
ATTATGTTGG GGGATAAGGA CAAAGTAACC GTTACTGACT TTGGCCTGGC AAAGCAAAAA 
CAAGAAAACA GTAAACTCAC CTCTGTGGTT GGAACAATCC TGTATTCTTG CCCCGAGGTA 
CTGAAGAGTG AGCCGTATGG GGAGAAGGCT GATGTCTGGG CAGTAGGCTG CATCCTTTAT 
CAGATGGCGA CTTTGAGTCC CCCCTTCTAC AGCACTAACA TGCTGTCCTT GGCTACAAAA 
ATAGTGGAGG CGGTATATGA ACCAGTCCCA GAAGGTATCT ACTCTGAAAA AGTAACAGAC 
ACCATCAGCA GGTGCCTCAC TCCTGATGCG GAAGCTCGTC CAGATATTGT AGAAGTCAGT 
TCGATGATAT CAGATGTCAT GATGAAATAT TTAGACAACT TATCTACATC CCAGTTGTCC 
TTGGAAAAGA AGCTAGAACG GGAACGAAGA CGCACACAAA GGTATTTTAT GGAAGCCAAC 
CGGAACACCG TCACATGTCA CCATGAGCTG GCTGTTCTAT CTCACGAGAC CTTTGAGAAG 
GCAAGTTTGA GTAGCAGCAG CAGTGGAGCA GCCAGCCTGA AAAGTGAACT TTCAGAAAGC 
GCAGACCTGC CCCCTGAAGG CTTCCAGGCC TCCTATGGTA AAGACGAAGA CAGGGCCTGT 
GACGAAATCC TGTCAGATGA TAACTTCAAC CTGGAAAATG CTGAGAAAGA TACATATTCA 
GAGGTAGATG ATGAATTGGA CATTTCGGAT AACTCCAGCA GCTCCAGTTC AAGCCCTCTG 
AAAGAATCTA CATTCAACAT TTTAAAGAGA AGTTTTAGTG CTTCAGGAGG AGAAAGACAA 
TCCCAAACAA GGGACTTCAC TGGAGGAACA GGATCAAGAC CAAGACCAGC TTTGCTGCCT 
CTTGACCTGC TTCTGAAAGT GCCACCCCAC ATGCTCAGGG CCCACATTAA GGAAATAGAG 
GCTGAGTTAG TGACAGGGTG GCAGTCCCAT AGCCTTCCTG CTGTGATTCT TCGAAATCTC 
AAAGATCATG GGCCACAGAT GGGCACATTC TTGTGGCAAG CATCAGCAGG AATTGCTGTG 
TCCCAGAGGA AAGTGCGTCA GATCAGTGAT CCTATTCAGC AGATATTAAT TCAGCTGCAC 
AAAATAATCT ATATCACACA GCTTCCTCCA GCTTTGCACC ACAATTTGAA AAGAAGGGTT 
ATAGAGAGAT TCAAGAAATC CCTCTTCAGC CAGCAGAGTA ACCCTTGTAA TTTGAAATCT 
GAAATTAAAA AGTTATCTCA GGGATCTCCA GAACCGATTG AGCCCAACTT TTTCACAGCA 
GATTACCATT TATTACATCG TTCATCCGGT GGAAACAGCC TGTCCCCAAA TGACCCTACA 
GGTTTACCAA CCAGCATTGA ATTGGAGGAA GGAATAACAT ATGAACAGAT GCAGACTGTG 
ATTGAAGAAG TCCTTGAGGA AAGTGGCTAT TACAATTTTA CATCTAACAG GTATCATTCC 
TATCCATGGG GGACCAAGAA TCACCCAACC AAAAGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016861252.1
CDS	372..3890
Translation	MPDQDKKVKTTEKSTDKQQEITIRDYSDLKRLRCLLNVQSSKQQLPAINFDSAQNSMTKSEPAIRAGGHRARGQWHESTEAVELENFSINYKNERNFSKHPQRKLFQEIFTALVKNRLISREWVNRAPSIHFLRVLICLRLLMRDPCYQEILHSLGGIENLAQYMEIVANEYLGYGEEQHTVDKLVNMTYIFQKLAAVKDQREWVTTSGAHKTLVNLLGARDTNVLLGSLLALASLAESQECREKISELNIVENLLMILHEYDLLSKRLTAELLRLLCAEPQVKEQVKLYEGIPVLLSLLHSDHLKLLWSIVWILVQVCEDPETSVEIRIWGGIKQLLHILQGDRNFVSDHSSIGSLSSANAAGRIQQLHLSEDLSPREIQENTFSLQAACCAALTELVLNDTNAHQVVQENGVYTIAKLILPNKQKNAAKSNLLQCYAFRALRFLFSMERNRPLFKRLFPTDLFEIFIDIGHYVRDISAYEELVSKLNLLVEDELKQIAENIESINQNKAPLKYIGNYAILDHLGSGAFGCVYKVRKHSGQNLLAMKEVNLHNPAFGKDKKDRDSSVRNIVSELTIIKEQLYHPNIVRYYKTFLENDRLYIVMELIEGAPLGEHFSSLKEKHHHFTEERLWKIFIQLCLALRYLHKEKRIVHRDLTPNNIMLGDKDKVTVTDFGLAKQKQENSKLTSVVGTILYSCPEVLKSEPYGEKADVWAVGCILYQMATLSPPFYSTNMLSLATKIVEAVYEPVPEGIYSEKVTDTISRCLTPDAEARPDIVEVSSMISDVMMKYLDNLSTSQLSLEKKLERERRRTQRYFMEANRNTVTCHHELAVLSHETFEKASLSSSSSGAASLKSELSESADLPPEGFQASYGKDEDRACDEILSDDNFNLENAEKDTYSEVDDELDISDNSSSSSSSPLKESTFNILKRSFSASGGERQSQTRDFTGGTGSRPRPALLPLDLLLKVPPHMLRAHIKEIEAELVTGWQSHSLPAVILRNLKDHGPQMGTFLWQASAGIAVSQRKVRQISDPIQQILIQLHKIIYITQLPPALHHNLKRRVIERFKKSLFSQQSNPCNLKSEIKKLSQGSPEPIEPNFFTADYHLLHRSSGGNSLSPNDPTGLPTSIELEEGITYEQMQTVIEEVLEESGYYNFTSNRYHSYPWGTKNHPTKR

Target ORF information:

RefSeq Version	XM_017005763.1
Organism	Homo sapiens(human)
Definition	Homo sapiens NIMA related kinase 10 (NEK10), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017005763.1

ORF Insert Sequence:

ATGCCTGATC AAGATAAAAA GGTGAAGACC ACAGAAAAAT CAACTGATAA ACAGCAAGAA 
ATCACCATCA GGGACTATTC AGATCTTAAA AGACTTCGGT GCCTTTTGAA CGTCCAATCA 
AGCAAACAAC AGCTTCCAGC CATTAACTTC GATAGTGCCC AAAATAGCAT GACGAAGTCT 
GAGCCCGCCA TCAGGGCGGG TGGACACAGA GCTCGGGGTC AGTGGCATGA ATCCACAGAA 
GCTGTTGAAC TTGAAAATTT TAGTATAAAC TACAAGAATG AGAGAAATTT CAGCAAACAT 
CCTCAGCGTA AACTATTTCA GGAGATCTTT ACCGCCTTGG TGAAAAATAG ACTCATAAGC 
AGAGAGTGGG TTAATCGAGC CCCATCTATT CATTTTCTGA GAGTGTTAAT CTGTCTGAGG 
CTACTAATGA GGGATCCATG TTATCAGGAA ATACTCCATA GCTTGGGTGG GATTGAAAAC 
CTAGCTCAGT ATATGGAGAT TGTAGCCAAT GAGTACCTCG GCTATGGAGA AGAGCAGCAC 
ACTGTGGACA AGCTGGTCAA CATGACATAT ATTTTTCAAA AACTTGCTGC AGTCAAAGAT 
CAAAGAGAAT GGGTCACCAC AAGTGGAGCC CACAAGACAT TAGTAAATTT ACTTGGTGCC 
CGAGATACTA ATGTTCTATT GGGTTCCCTT CTGGCTCTGG CTAGTTTAGC AGAAAGTCAA 
GAATGTAGGG AGAAGATAAG TGAACTCAAC ATTGTAGAAA ATCTGTTGAT GATTTTACAT 
GAATATGACT TGCTTTCTAA AAGACTAACA GCGGAGTTGC TGCGCCTACT TTGTGCAGAG 
CCCCAGGTGA AAGAGCAGGT GAAGCTCTAT GAGGGGATAC CGGTCCTCCT CAGTCTGCTC 
CACTCTGACC ACTTGAAGCT CCTCTGGAGC ATTGTCTGGA TTCTGGTACA GGTTTGTGAG 
GACCCTGAGA CCAGCGTGGA AATTCGCATT TGGGGAGGCA TCAAACAGCT TCTTCATATT 
TTACAAGGAG ACAGAAATTT TGTTTCTGAT CACTCCTCCA TTGGAAGCCT GTCCAGTGCA 
AATGCTGCAG GCCGAATCCA GCAGCTTCAT TTATCAGAAG ACTTGAGCCC TAGGGAAATA 
CAAGAAAATA CTTTCTCACT TCAAGCAGCC TGCTGTGCTG CCCTCACTGA GCTGGTGCTC 
AATGACACCA ATGCCCACCA GGTGGTTCAG GAAAATGGTG TATATACAAT AGCAAAATTA 
ATTTTACCAA ATAAGCAAAA GAATGCAGCA AAAAGTAATC TATTACAGTG TTATGCTTTC 
AGAGCCTTGA GATTTCTCTT CAGTATGGAA AGAAACAGAC CACTCTTTAA AAGACTTTTC 
CCCACAGACT TGTTTGAGAT CTTCATTGAC ATAGGGCATT ATGTACGTGA TATCAGTGCT 
TATGAAGAAT TGGTATCCAA GCTGAATTTA TTAGTGGAGG ATGAACTGAA GCAAATTGCT 
GAAAATATTG AAAGCATTAA TCAGAACAAA GCTCCTTTGA AATATATAGG CAACTATGCA 
ATTTTGGATC ATCTTGGAAG TGGAGCTTTT GGCTGTGTTT ACAAGGTTAG AAAGCATAGT 
GGTCAAAATC TTTTAGCAAT GAAAGAGGTC AATTTACATA ACCCAGCATT TGGAAAGGAT 
AAGAAAGATC GAGACAGCAG CGTAAGGAAT ATTGTTTCTG AATTAACAAT AATTAAAGAG 
CAGCTTTATC ATCCCAACAT TGTACGTTAT TACAAAACAT TTCTGGAAAA TGATAGGTTG 
TACATAGTTA TGGAGCTGAT AGAAGGAGCC CCGCTTGGAG AGCATTTCAG TTCTTTGAAG 
GAAAAACATC ACCATTTTAC TGAAGAAAGA CTATGGAAAA TATTTATACA GCTGTGCTTA 
GCTCTTCGAT ACTTACACAA GGAGAAGAGG ATTGTCCATA GAGATCTGAC ACCAAACAAC 
ATTATGTTGG GGGATAAGGA CAAAGTAACC GTTACTGACT TTGGCCTGGC AAAGCAAAAA 
CAAGAAAACA GTAAACTCAC CTCTGTGGTT GGAACAATCC TGTATTCTTG CCCCGAGGTA 
CTGAAGAGTG AGCCGTATGG GGAGAAGGCT GATGTCTGGG CAGTAGGCTG CATCCTTTAT 
CAGATGGCGA CTTTGAGTCC CCCCTTCTAC AGCACTAACA TGCTGTCCTT GGCTACAAAA 
ATAGTGGAGG CGGTATATGA ACCAGTCCCA GAAGGTATCT ACTCTGAAAA AGTAACAGAC 
ACCATCAGCA GGTGCCTCAC TCCTGATGCG GAAGCTCGTC CAGATATTGT AGAAGTCAGT 
TCGATGATAT CAGATGTCAT GATGAAATAT TTAGACAACT TATCTACATC CCAGTTGTCC 
TTGGAAAAGA AGCTAGAACG GGAACGAAGA CGCACACAAA GGTATTTTAT GGAAGCCAAC 
CGGAACACCG TCACATGTCA CCATGAGCTG GCTGTTCTAT CTCACGAGAC CTTTGAGAAG 
GCAAGTTTGA GTAGCAGCAG CAGTGGAGCA GCCAGCCTGA AAAGTGAACT TTCAGAAAGC 
GCAGACCTGC CCCCTGAAGG CTTCCAGGCC TCCTATGGTA AAGACGAAGA CAGGGCCTGT 
GACGAAATCC TGTCAGATGA TAACTTCAAC CTGGAAAATG CTGAGAAAGA TACATATTCA 
GAGGTAGATG ATGAATTGGA CATTTCGGAT AACTCCAGCA GCTCCAGTTC AAGCCCTCTG 
AAAGAATCTA CATTCAACAT TTTAAAGAGA AGTTTTAGTG CTTCAGGAGG AGAAAGACAA 
TCCCAAACAA GGGACTTCAC TGGAGGAACA GGATCAAGAC CAAGACCAGC TTTGCTGCCT 
CTTGACCTGC TTCTGAAAGT GCCACCCCAC ATGCTCAGGG CCCACATTAA GGAAATAGAG 
GCTGAGTTAG TGACAGGGTG GCAGTCCCAT AGCCTTCCTG CTGTGATTCT TCGAAATCTC 
AAAGATCATG GGCCACAGAT GGGCACATTC TTGTGGCAAG CATCAGCAGG AATTGCTGTG 
TCCCAGAGGA AAGTGCGTCA GATCAGTGAT CCTATTCAGC AGATATTAAT TCAGCTGCAC 
AAAATAATCT ATATCACACA GCTTCCTCCA GCTTTGCACC ACAATTTGAA AAGAAGGGTT 
ATAGAGAGAT TCAAGAAATC CCTCTTCAGC CAGCAGAGTA ACCCTTGTAA TTTGAAATCT 
GAAATTAAAA AGTTATCTCA GGGATCTCCA GAACCGATTG AGCCCAACTT TTTCACAGCA 
GATTACCATT TATTACATCG TTCATCCGGT GGAAACAGCC TGTCCCCAAA TGACCCTACA 
GGTTTACCAA CCAGCATTGA ATTGGAGGAA GGAATAACAT ATGAACAGAT GCAGACTGTG 
ATTGAAGAAG TCCTTGAGGA AAGTGGCTAT TACAATTTTA CATCTAACAG GTATCATTCC 
TATCCATGGG GGACCAAGAA TCACCCAACC AAAAGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NEK10 ( XM_017005762.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016861251.1 Homo sapiens NIMA related kinase 10 (NEK10), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OHu36991
Clone ID Related Accession (Same CDS sequence)	XM_017005764.1 , XM_017005762.2 , NM_152534.4 , XM_017005761.1 , XM_017005763.1 , XM_017005762.1 , XM_017005761.2 , XM_017005765.1
Accession Version	XM_017005762.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3519bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	serine/threonine-protein kinase Nek10 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000003.12) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCTGATC AAGATAAAAA GGTGAAGACC ACAGAAAAAT CAACTGATAA ACAGCAAGAA 
ATCACCATCA GGGACTATTC AGATCTTAAA AGACTTCGGT GCCTTTTGAA CGTCCAATCA 
AGCAAACAAC AGCTTCCAGC CATTAACTTC GATAGTGCCC AAAATAGCAT GACGAAGTCT 
GAGCCCGCCA TCAGGGCGGG TGGACACAGA GCTCGGGGTC AGTGGCATGA ATCCACAGAA 
GCTGTTGAAC TTGAAAATTT TAGTATAAAC TACAAGAATG AGAGAAATTT CAGCAAACAT 
CCTCAGCGTA AACTATTTCA GGAGATCTTT ACCGCCTTGG TGAAAAATAG ACTCATAAGC 
AGAGAGTGGG TTAATCGAGC CCCATCTATT CATTTTCTGA GAGTGTTAAT CTGTCTGAGG 
CTACTAATGA GGGATCCATG TTATCAGGAA ATACTCCATA GCTTGGGTGG GATTGAAAAC 
CTAGCTCAGT ATATGGAGAT TGTAGCCAAT GAGTACCTCG GCTATGGAGA AGAGCAGCAC 
ACTGTGGACA AGCTGGTCAA CATGACATAT ATTTTTCAAA AACTTGCTGC AGTCAAAGAT 
CAAAGAGAAT GGGTCACCAC AAGTGGAGCC CACAAGACAT TAGTAAATTT ACTTGGTGCC 
CGAGATACTA ATGTTCTATT GGGTTCCCTT CTGGCTCTGG CTAGTTTAGC AGAAAGTCAA 
GAATGTAGGG AGAAGATAAG TGAACTCAAC ATTGTAGAAA ATCTGTTGAT GATTTTACAT 
GAATATGACT TGCTTTCTAA AAGACTAACA GCGGAGTTGC TGCGCCTACT TTGTGCAGAG 
CCCCAGGTGA AAGAGCAGGT GAAGCTCTAT GAGGGGATAC CGGTCCTCCT CAGTCTGCTC 
CACTCTGACC ACTTGAAGCT CCTCTGGAGC ATTGTCTGGA TTCTGGTACA GGTTTGTGAG 
GACCCTGAGA CCAGCGTGGA AATTCGCATT TGGGGAGGCA TCAAACAGCT TCTTCATATT 
TTACAAGGAG ACAGAAATTT TGTTTCTGAT CACTCCTCCA TTGGAAGCCT GTCCAGTGCA 
AATGCTGCAG GCCGAATCCA GCAGCTTCAT TTATCAGAAG ACTTGAGCCC TAGGGAAATA 
CAAGAAAATA CTTTCTCACT TCAAGCAGCC TGCTGTGCTG CCCTCACTGA GCTGGTGCTC 
AATGACACCA ATGCCCACCA GGTGGTTCAG GAAAATGGTG TATATACAAT AGCAAAATTA 
ATTTTACCAA ATAAGCAAAA GAATGCAGCA AAAAGTAATC TATTACAGTG TTATGCTTTC 
AGAGCCTTGA GATTTCTCTT CAGTATGGAA AGAAACAGAC CACTCTTTAA AAGACTTTTC 
CCCACAGACT TGTTTGAGAT CTTCATTGAC ATAGGGCATT ATGTACGTGA TATCAGTGCT 
TATGAAGAAT TGGTATCCAA GCTGAATTTA TTAGTGGAGG ATGAACTGAA GCAAATTGCT 
GAAAATATTG AAAGCATTAA TCAGAACAAA GCTCCTTTGA AATATATAGG CAACTATGCA 
ATTTTGGATC ATCTTGGAAG TGGAGCTTTT GGCTGTGTTT ACAAGGTTAG AAAGCATAGT 
GGTCAAAATC TTTTAGCAAT GAAAGAGGTC AATTTACATA ACCCAGCATT TGGAAAGGAT 
AAGAAAGATC GAGACAGCAG CGTAAGGAAT ATTGTTTCTG AATTAACAAT AATTAAAGAG 
CAGCTTTATC ATCCCAACAT TGTACGTTAT TACAAAACAT TTCTGGAAAA TGATAGGTTG 
TACATAGTTA TGGAGCTGAT AGAAGGAGCC CCGCTTGGAG AGCATTTCAG TTCTTTGAAG 
GAAAAACATC ACCATTTTAC TGAAGAAAGA CTATGGAAAA TATTTATACA GCTGTGCTTA 
GCTCTTCGAT ACTTACACAA GGAGAAGAGG ATTGTCCATA GAGATCTGAC ACCAAACAAC 
ATTATGTTGG GGGATAAGGA CAAAGTAACC GTTACTGACT TTGGCCTGGC AAAGCAAAAA 
CAAGAAAACA GTAAACTCAC CTCTGTGGTT GGAACAATCC TGTATTCTTG CCCCGAGGTA 
CTGAAGAGTG AGCCGTATGG GGAGAAGGCT GATGTCTGGG CAGTAGGCTG CATCCTTTAT 
CAGATGGCGA CTTTGAGTCC CCCCTTCTAC AGCACTAACA TGCTGTCCTT GGCTACAAAA 
ATAGTGGAGG CGGTATATGA ACCAGTCCCA GAAGGTATCT ACTCTGAAAA AGTAACAGAC 
ACCATCAGCA GGTGCCTCAC TCCTGATGCG GAAGCTCGTC CAGATATTGT AGAAGTCAGT 
TCGATGATAT CAGATGTCAT GATGAAATAT TTAGACAACT TATCTACATC CCAGTTGTCC 
TTGGAAAAGA AGCTAGAACG GGAACGAAGA CGCACACAAA GGTATTTTAT GGAAGCCAAC 
CGGAACACCG TCACATGTCA CCATGAGCTG GCTGTTCTAT CTCACGAGAC CTTTGAGAAG 
GCAAGTTTGA GTAGCAGCAG CAGTGGAGCA GCCAGCCTGA AAAGTGAACT TTCAGAAAGC 
GCAGACCTGC CCCCTGAAGG CTTCCAGGCC TCCTATGGTA AAGACGAAGA CAGGGCCTGT 
GACGAAATCC TGTCAGATGA TAACTTCAAC CTGGAAAATG CTGAGAAAGA TACATATTCA 
GAGGTAGATG ATGAATTGGA CATTTCGGAT AACTCCAGCA GCTCCAGTTC AAGCCCTCTG 
AAAGAATCTA CATTCAACAT TTTAAAGAGA AGTTTTAGTG CTTCAGGAGG AGAAAGACAA 
TCCCAAACAA GGGACTTCAC TGGAGGAACA GGATCAAGAC CAAGACCAGC TTTGCTGCCT 
CTTGACCTGC TTCTGAAAGT GCCACCCCAC ATGCTCAGGG CCCACATTAA GGAAATAGAG 
GCTGAGTTAG TGACAGGGTG GCAGTCCCAT AGCCTTCCTG CTGTGATTCT TCGAAATCTC 
AAAGATCATG GGCCACAGAT GGGCACATTC TTGTGGCAAG CATCAGCAGG AATTGCTGTG 
TCCCAGAGGA AAGTGCGTCA GATCAGTGAT CCTATTCAGC AGATATTAAT TCAGCTGCAC 
AAAATAATCT ATATCACACA GCTTCCTCCA GCTTTGCACC ACAATTTGAA AAGAAGGGTT 
ATAGAGAGAT TCAAGAAATC CCTCTTCAGC CAGCAGAGTA ACCCTTGTAA TTTGAAATCT 
GAAATTAAAA AGTTATCTCA GGGATCTCCA GAACCGATTG AGCCCAACTT TTTCACAGCA 
GATTACCATT TATTACATCG TTCATCCGGT GGAAACAGCC TGTCCCCAAA TGACCCTACA 
GGTTTACCAA CCAGCATTGA ATTGGAGGAA GGAATAACAT ATGAACAGAT GCAGACTGTG 
ATTGAAGAAG TCCTTGAGGA AAGTGGCTAT TACAATTTTA CATCTAACAG GTATCATTCC 
TATCCATGGG GGACCAAGAA TCACCCAACC AAAAGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016861251.1
CDS	391..3909
Translation	MPDQDKKVKTTEKSTDKQQEITIRDYSDLKRLRCLLNVQSSKQQLPAINFDSAQNSMTKSEPAIRAGGHRARGQWHESTEAVELENFSINYKNERNFSKHPQRKLFQEIFTALVKNRLISREWVNRAPSIHFLRVLICLRLLMRDPCYQEILHSLGGIENLAQYMEIVANEYLGYGEEQHTVDKLVNMTYIFQKLAAVKDQREWVTTSGAHKTLVNLLGARDTNVLLGSLLALASLAESQECREKISELNIVENLLMILHEYDLLSKRLTAELLRLLCAEPQVKEQVKLYEGIPVLLSLLHSDHLKLLWSIVWILVQVCEDPETSVEIRIWGGIKQLLHILQGDRNFVSDHSSIGSLSSANAAGRIQQLHLSEDLSPREIQENTFSLQAACCAALTELVLNDTNAHQVVQENGVYTIAKLILPNKQKNAAKSNLLQCYAFRALRFLFSMERNRPLFKRLFPTDLFEIFIDIGHYVRDISAYEELVSKLNLLVEDELKQIAENIESINQNKAPLKYIGNYAILDHLGSGAFGCVYKVRKHSGQNLLAMKEVNLHNPAFGKDKKDRDSSVRNIVSELTIIKEQLYHPNIVRYYKTFLENDRLYIVMELIEGAPLGEHFSSLKEKHHHFTEERLWKIFIQLCLALRYLHKEKRIVHRDLTPNNIMLGDKDKVTVTDFGLAKQKQENSKLTSVVGTILYSCPEVLKSEPYGEKADVWAVGCILYQMATLSPPFYSTNMLSLATKIVEAVYEPVPEGIYSEKVTDTISRCLTPDAEARPDIVEVSSMISDVMMKYLDNLSTSQLSLEKKLERERRRTQRYFMEANRNTVTCHHELAVLSHETFEKASLSSSSSGAASLKSELSESADLPPEGFQASYGKDEDRACDEILSDDNFNLENAEKDTYSEVDDELDISDNSSSSSSSPLKESTFNILKRSFSASGGERQSQTRDFTGGTGSRPRPALLPLDLLLKVPPHMLRAHIKEIEAELVTGWQSHSLPAVILRNLKDHGPQMGTFLWQASAGIAVSQRKVRQISDPIQQILIQLHKIIYITQLPPALHHNLKRRVIERFKKSLFSQQSNPCNLKSEIKKLSQGSPEPIEPNFFTADYHLLHRSSGGNSLSPNDPTGLPTSIELEEGITYEQMQTVIEEVLEESGYYNFTSNRYHSYPWGTKNHPTKR

Target ORF information:

RefSeq Version	XM_017005762.1
Organism	Homo sapiens(human)
Definition	Homo sapiens NIMA related kinase 10 (NEK10), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017005762.1

ORF Insert Sequence:

ATGCCTGATC AAGATAAAAA GGTGAAGACC ACAGAAAAAT CAACTGATAA ACAGCAAGAA 
ATCACCATCA GGGACTATTC AGATCTTAAA AGACTTCGGT GCCTTTTGAA CGTCCAATCA 
AGCAAACAAC AGCTTCCAGC CATTAACTTC GATAGTGCCC AAAATAGCAT GACGAAGTCT 
GAGCCCGCCA TCAGGGCGGG TGGACACAGA GCTCGGGGTC AGTGGCATGA ATCCACAGAA 
GCTGTTGAAC TTGAAAATTT TAGTATAAAC TACAAGAATG AGAGAAATTT CAGCAAACAT 
CCTCAGCGTA AACTATTTCA GGAGATCTTT ACCGCCTTGG TGAAAAATAG ACTCATAAGC 
AGAGAGTGGG TTAATCGAGC CCCATCTATT CATTTTCTGA GAGTGTTAAT CTGTCTGAGG 
CTACTAATGA GGGATCCATG TTATCAGGAA ATACTCCATA GCTTGGGTGG GATTGAAAAC 
CTAGCTCAGT ATATGGAGAT TGTAGCCAAT GAGTACCTCG GCTATGGAGA AGAGCAGCAC 
ACTGTGGACA AGCTGGTCAA CATGACATAT ATTTTTCAAA AACTTGCTGC AGTCAAAGAT 
CAAAGAGAAT GGGTCACCAC AAGTGGAGCC CACAAGACAT TAGTAAATTT ACTTGGTGCC 
CGAGATACTA ATGTTCTATT GGGTTCCCTT CTGGCTCTGG CTAGTTTAGC AGAAAGTCAA 
GAATGTAGGG AGAAGATAAG TGAACTCAAC ATTGTAGAAA ATCTGTTGAT GATTTTACAT 
GAATATGACT TGCTTTCTAA AAGACTAACA GCGGAGTTGC TGCGCCTACT TTGTGCAGAG 
CCCCAGGTGA AAGAGCAGGT GAAGCTCTAT GAGGGGATAC CGGTCCTCCT CAGTCTGCTC 
CACTCTGACC ACTTGAAGCT CCTCTGGAGC ATTGTCTGGA TTCTGGTACA GGTTTGTGAG 
GACCCTGAGA CCAGCGTGGA AATTCGCATT TGGGGAGGCA TCAAACAGCT TCTTCATATT 
TTACAAGGAG ACAGAAATTT TGTTTCTGAT CACTCCTCCA TTGGAAGCCT GTCCAGTGCA 
AATGCTGCAG GCCGAATCCA GCAGCTTCAT TTATCAGAAG ACTTGAGCCC TAGGGAAATA 
CAAGAAAATA CTTTCTCACT TCAAGCAGCC TGCTGTGCTG CCCTCACTGA GCTGGTGCTC 
AATGACACCA ATGCCCACCA GGTGGTTCAG GAAAATGGTG TATATACAAT AGCAAAATTA 
ATTTTACCAA ATAAGCAAAA GAATGCAGCA AAAAGTAATC TATTACAGTG TTATGCTTTC 
AGAGCCTTGA GATTTCTCTT CAGTATGGAA AGAAACAGAC CACTCTTTAA AAGACTTTTC 
CCCACAGACT TGTTTGAGAT CTTCATTGAC ATAGGGCATT ATGTACGTGA TATCAGTGCT 
TATGAAGAAT TGGTATCCAA GCTGAATTTA TTAGTGGAGG ATGAACTGAA GCAAATTGCT 
GAAAATATTG AAAGCATTAA TCAGAACAAA GCTCCTTTGA AATATATAGG CAACTATGCA 
ATTTTGGATC ATCTTGGAAG TGGAGCTTTT GGCTGTGTTT ACAAGGTTAG AAAGCATAGT 
GGTCAAAATC TTTTAGCAAT GAAAGAGGTC AATTTACATA ACCCAGCATT TGGAAAGGAT 
AAGAAAGATC GAGACAGCAG CGTAAGGAAT ATTGTTTCTG AATTAACAAT AATTAAAGAG 
CAGCTTTATC ATCCCAACAT TGTACGTTAT TACAAAACAT TTCTGGAAAA TGATAGGTTG 
TACATAGTTA TGGAGCTGAT AGAAGGAGCC CCGCTTGGAG AGCATTTCAG TTCTTTGAAG 
GAAAAACATC ACCATTTTAC TGAAGAAAGA CTATGGAAAA TATTTATACA GCTGTGCTTA 
GCTCTTCGAT ACTTACACAA GGAGAAGAGG ATTGTCCATA GAGATCTGAC ACCAAACAAC 
ATTATGTTGG GGGATAAGGA CAAAGTAACC GTTACTGACT TTGGCCTGGC AAAGCAAAAA 
CAAGAAAACA GTAAACTCAC CTCTGTGGTT GGAACAATCC TGTATTCTTG CCCCGAGGTA 
CTGAAGAGTG AGCCGTATGG GGAGAAGGCT GATGTCTGGG CAGTAGGCTG CATCCTTTAT 
CAGATGGCGA CTTTGAGTCC CCCCTTCTAC AGCACTAACA TGCTGTCCTT GGCTACAAAA 
ATAGTGGAGG CGGTATATGA ACCAGTCCCA GAAGGTATCT ACTCTGAAAA AGTAACAGAC 
ACCATCAGCA GGTGCCTCAC TCCTGATGCG GAAGCTCGTC CAGATATTGT AGAAGTCAGT 
TCGATGATAT CAGATGTCAT GATGAAATAT TTAGACAACT TATCTACATC CCAGTTGTCC 
TTGGAAAAGA AGCTAGAACG GGAACGAAGA CGCACACAAA GGTATTTTAT GGAAGCCAAC 
CGGAACACCG TCACATGTCA CCATGAGCTG GCTGTTCTAT CTCACGAGAC CTTTGAGAAG 
GCAAGTTTGA GTAGCAGCAG CAGTGGAGCA GCCAGCCTGA AAAGTGAACT TTCAGAAAGC 
GCAGACCTGC CCCCTGAAGG CTTCCAGGCC TCCTATGGTA AAGACGAAGA CAGGGCCTGT 
GACGAAATCC TGTCAGATGA TAACTTCAAC CTGGAAAATG CTGAGAAAGA TACATATTCA 
GAGGTAGATG ATGAATTGGA CATTTCGGAT AACTCCAGCA GCTCCAGTTC AAGCCCTCTG 
AAAGAATCTA CATTCAACAT TTTAAAGAGA AGTTTTAGTG CTTCAGGAGG AGAAAGACAA 
TCCCAAACAA GGGACTTCAC TGGAGGAACA GGATCAAGAC CAAGACCAGC TTTGCTGCCT 
CTTGACCTGC TTCTGAAAGT GCCACCCCAC ATGCTCAGGG CCCACATTAA GGAAATAGAG 
GCTGAGTTAG TGACAGGGTG GCAGTCCCAT AGCCTTCCTG CTGTGATTCT TCGAAATCTC 
AAAGATCATG GGCCACAGAT GGGCACATTC TTGTGGCAAG CATCAGCAGG AATTGCTGTG 
TCCCAGAGGA AAGTGCGTCA GATCAGTGAT CCTATTCAGC AGATATTAAT TCAGCTGCAC 
AAAATAATCT ATATCACACA GCTTCCTCCA GCTTTGCACC ACAATTTGAA AAGAAGGGTT 
ATAGAGAGAT TCAAGAAATC CCTCTTCAGC CAGCAGAGTA ACCCTTGTAA TTTGAAATCT 
GAAATTAAAA AGTTATCTCA GGGATCTCCA GAACCGATTG AGCCCAACTT TTTCACAGCA 
GATTACCATT TATTACATCG TTCATCCGGT GGAAACAGCC TGTCCCCAAA TGACCCTACA 
GGTTTACCAA CCAGCATTGA ATTGGAGGAA GGAATAACAT ATGAACAGAT GCAGACTGTG 
ATTGAAGAAG TCCTTGAGGA AAGTGGCTAT TACAATTTTA CATCTAACAG GTATCATTCC 
TATCCATGGG GGACCAAGAA TCACCCAACC AAAAGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NEK10 ( XM_017005761.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_016861250.1 Homo sapiens NIMA related kinase 10 (NEK10), transcript variant X2, mRNA.

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CloneID	OHu36991
Clone ID Related Accession (Same CDS sequence)	XM_017005764.1 , XM_017005762.2 , NM_152534.4 , XM_017005761.1 , XM_017005763.1 , XM_017005762.1 , XM_017005761.2 , XM_017005765.1
Accession Version	XM_017005761.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3519bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	serine/threonine-protein kinase Nek10 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000003.12) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_017005761.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCTGATC AAGATAAAAA GGTGAAGACC ACAGAAAAAT CAACTGATAA ACAGCAAGAA 
ATCACCATCA GGGACTATTC AGATCTTAAA AGACTTCGGT GCCTTTTGAA CGTCCAATCA 
AGCAAACAAC AGCTTCCAGC CATTAACTTC GATAGTGCCC AAAATAGCAT GACGAAGTCT 
GAGCCCGCCA TCAGGGCGGG TGGACACAGA GCTCGGGGTC AGTGGCATGA ATCCACAGAA 
GCTGTTGAAC TTGAAAATTT TAGTATAAAC TACAAGAATG AGAGAAATTT CAGCAAACAT 
CCTCAGCGTA AACTATTTCA GGAGATCTTT ACCGCCTTGG TGAAAAATAG ACTCATAAGC 
AGAGAGTGGG TTAATCGAGC CCCATCTATT CATTTTCTGA GAGTGTTAAT CTGTCTGAGG 
CTACTAATGA GGGATCCATG TTATCAGGAA ATACTCCATA GCTTGGGTGG GATTGAAAAC 
CTAGCTCAGT ATATGGAGAT TGTAGCCAAT GAGTACCTCG GCTATGGAGA AGAGCAGCAC 
ACTGTGGACA AGCTGGTCAA CATGACATAT ATTTTTCAAA AACTTGCTGC AGTCAAAGAT 
CAAAGAGAAT GGGTCACCAC AAGTGGAGCC CACAAGACAT TAGTAAATTT ACTTGGTGCC 
CGAGATACTA ATGTTCTATT GGGTTCCCTT CTGGCTCTGG CTAGTTTAGC AGAAAGTCAA 
GAATGTAGGG AGAAGATAAG TGAACTCAAC ATTGTAGAAA ATCTGTTGAT GATTTTACAT 
GAATATGACT TGCTTTCTAA AAGACTAACA GCGGAGTTGC TGCGCCTACT TTGTGCAGAG 
CCCCAGGTGA AAGAGCAGGT GAAGCTCTAT GAGGGGATAC CGGTCCTCCT CAGTCTGCTC 
CACTCTGACC ACTTGAAGCT CCTCTGGAGC ATTGTCTGGA TTCTGGTACA GGTTTGTGAG 
GACCCTGAGA CCAGCGTGGA AATTCGCATT TGGGGAGGCA TCAAACAGCT TCTTCATATT 
TTACAAGGAG ACAGAAATTT TGTTTCTGAT CACTCCTCCA TTGGAAGCCT GTCCAGTGCA 
AATGCTGCAG GCCGAATCCA GCAGCTTCAT TTATCAGAAG ACTTGAGCCC TAGGGAAATA 
CAAGAAAATA CTTTCTCACT TCAAGCAGCC TGCTGTGCTG CCCTCACTGA GCTGGTGCTC 
AATGACACCA ATGCCCACCA GGTGGTTCAG GAAAATGGTG TATATACAAT AGCAAAATTA 
ATTTTACCAA ATAAGCAAAA GAATGCAGCA AAAAGTAATC TATTACAGTG TTATGCTTTC 
AGAGCCTTGA GATTTCTCTT CAGTATGGAA AGAAACAGAC CACTCTTTAA AAGACTTTTC 
CCCACAGACT TGTTTGAGAT CTTCATTGAC ATAGGGCATT ATGTACGTGA TATCAGTGCT 
TATGAAGAAT TGGTATCCAA GCTGAATTTA TTAGTGGAGG ATGAACTGAA GCAAATTGCT 
GAAAATATTG AAAGCATTAA TCAGAACAAA GCTCCTTTGA AATATATAGG CAACTATGCA 
ATTTTGGATC ATCTTGGAAG TGGAGCTTTT GGCTGTGTTT ACAAGGTTAG AAAGCATAGT 
GGTCAAAATC TTTTAGCAAT GAAAGAGGTC AATTTACATA ACCCAGCATT TGGAAAGGAT 
AAGAAAGATC GAGACAGCAG CGTAAGGAAT ATTGTTTCTG AATTAACAAT AATTAAAGAG 
CAGCTTTATC ATCCCAACAT TGTACGTTAT TACAAAACAT TTCTGGAAAA TGATAGGTTG 
TACATAGTTA TGGAGCTGAT AGAAGGAGCC CCGCTTGGAG AGCATTTCAG TTCTTTGAAG 
GAAAAACATC ACCATTTTAC TGAAGAAAGA CTATGGAAAA TATTTATACA GCTGTGCTTA 
GCTCTTCGAT ACTTACACAA GGAGAAGAGG ATTGTCCATA GAGATCTGAC ACCAAACAAC 
ATTATGTTGG GGGATAAGGA CAAAGTAACC GTTACTGACT TTGGCCTGGC AAAGCAAAAA 
CAAGAAAACA GTAAACTCAC CTCTGTGGTT GGAACAATCC TGTATTCTTG CCCCGAGGTA 
CTGAAGAGTG AGCCGTATGG GGAGAAGGCT GATGTCTGGG CAGTAGGCTG CATCCTTTAT 
CAGATGGCGA CTTTGAGTCC CCCCTTCTAC AGCACTAACA TGCTGTCCTT GGCTACAAAA 
ATAGTGGAGG CGGTATATGA ACCAGTCCCA GAAGGTATCT ACTCTGAAAA AGTAACAGAC 
ACCATCAGCA GGTGCCTCAC TCCTGATGCG GAAGCTCGTC CAGATATTGT AGAAGTCAGT 
TCGATGATAT CAGATGTCAT GATGAAATAT TTAGACAACT TATCTACATC CCAGTTGTCC 
TTGGAAAAGA AGCTAGAACG GGAACGAAGA CGCACACAAA GGTATTTTAT GGAAGCCAAC 
CGGAACACCG TCACATGTCA CCATGAGCTG GCTGTTCTAT CTCACGAGAC CTTTGAGAAG 
GCAAGTTTGA GTAGCAGCAG CAGTGGAGCA GCCAGCCTGA AAAGTGAACT TTCAGAAAGC 
GCAGACCTGC CCCCTGAAGG CTTCCAGGCC TCCTATGGTA AAGACGAAGA CAGGGCCTGT 
GACGAAATCC TGTCAGATGA TAACTTCAAC CTGGAAAATG CTGAGAAAGA TACATATTCA 
GAGGTAGATG ATGAATTGGA CATTTCGGAT AACTCCAGCA GCTCCAGTTC AAGCCCTCTG 
AAAGAATCTA CATTCAACAT TTTAAAGAGA AGTTTTAGTG CTTCAGGAGG AGAAAGACAA 
TCCCAAACAA GGGACTTCAC TGGAGGAACA GGATCAAGAC CAAGACCAGC TTTGCTGCCT 
CTTGACCTGC TTCTGAAAGT GCCACCCCAC ATGCTCAGGG CCCACATTAA GGAAATAGAG 
GCTGAGTTAG TGACAGGGTG GCAGTCCCAT AGCCTTCCTG CTGTGATTCT TCGAAATCTC 
AAAGATCATG GGCCACAGAT GGGCACATTC TTGTGGCAAG CATCAGCAGG AATTGCTGTG 
TCCCAGAGGA AAGTGCGTCA GATCAGTGAT CCTATTCAGC AGATATTAAT TCAGCTGCAC 
AAAATAATCT ATATCACACA GCTTCCTCCA GCTTTGCACC ACAATTTGAA AAGAAGGGTT 
ATAGAGAGAT TCAAGAAATC CCTCTTCAGC CAGCAGAGTA ACCCTTGTAA TTTGAAATCT 
GAAATTAAAA AGTTATCTCA GGGATCTCCA GAACCGATTG AGCCCAACTT TTTCACAGCA 
GATTACCATT TATTACATCG TTCATCCGGT GGAAACAGCC TGTCCCCAAA TGACCCTACA 
GGTTTACCAA CCAGCATTGA ATTGGAGGAA GGAATAACAT ATGAACAGAT GCAGACTGTG 
ATTGAAGAAG TCCTTGAGGA AAGTGGCTAT TACAATTTTA CATCTAACAG GTATCATTCC 
TATCCATGGG GGACCAAGAA TCACCCAACC AAAAGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016861250.1
CDS	274..3792
Translation	MPDQDKKVKTTEKSTDKQQEITIRDYSDLKRLRCLLNVQSSKQQLPAINFDSAQNSMTKSEPAIRAGGHRARGQWHESTEAVELENFSINYKNERNFSKHPQRKLFQEIFTALVKNRLISREWVNRAPSIHFLRVLICLRLLMRDPCYQEILHSLGGIENLAQYMEIVANEYLGYGEEQHTVDKLVNMTYIFQKLAAVKDQREWVTTSGAHKTLVNLLGARDTNVLLGSLLALASLAESQECREKISELNIVENLLMILHEYDLLSKRLTAELLRLLCAEPQVKEQVKLYEGIPVLLSLLHSDHLKLLWSIVWILVQVCEDPETSVEIRIWGGIKQLLHILQGDRNFVSDHSSIGSLSSANAAGRIQQLHLSEDLSPREIQENTFSLQAACCAALTELVLNDTNAHQVVQENGVYTIAKLILPNKQKNAAKSNLLQCYAFRALRFLFSMERNRPLFKRLFPTDLFEIFIDIGHYVRDISAYEELVSKLNLLVEDELKQIAENIESINQNKAPLKYIGNYAILDHLGSGAFGCVYKVRKHSGQNLLAMKEVNLHNPAFGKDKKDRDSSVRNIVSELTIIKEQLYHPNIVRYYKTFLENDRLYIVMELIEGAPLGEHFSSLKEKHHHFTEERLWKIFIQLCLALRYLHKEKRIVHRDLTPNNIMLGDKDKVTVTDFGLAKQKQENSKLTSVVGTILYSCPEVLKSEPYGEKADVWAVGCILYQMATLSPPFYSTNMLSLATKIVEAVYEPVPEGIYSEKVTDTISRCLTPDAEARPDIVEVSSMISDVMMKYLDNLSTSQLSLEKKLERERRRTQRYFMEANRNTVTCHHELAVLSHETFEKASLSSSSSGAASLKSELSESADLPPEGFQASYGKDEDRACDEILSDDNFNLENAEKDTYSEVDDELDISDNSSSSSSSPLKESTFNILKRSFSASGGERQSQTRDFTGGTGSRPRPALLPLDLLLKVPPHMLRAHIKEIEAELVTGWQSHSLPAVILRNLKDHGPQMGTFLWQASAGIAVSQRKVRQISDPIQQILIQLHKIIYITQLPPALHHNLKRRVIERFKKSLFSQQSNPCNLKSEIKKLSQGSPEPIEPNFFTADYHLLHRSSGGNSLSPNDPTGLPTSIELEEGITYEQMQTVIEEVLEESGYYNFTSNRYHSYPWGTKNHPTKR

Target ORF information:

RefSeq Version	XM_017005761.2
Organism	Homo sapiens(human)
Definition	Homo sapiens NIMA related kinase 10 (NEK10), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017005761.2

ORF Insert Sequence:

ATGCCTGATC AAGATAAAAA GGTGAAGACC ACAGAAAAAT CAACTGATAA ACAGCAAGAA 
ATCACCATCA GGGACTATTC AGATCTTAAA AGACTTCGGT GCCTTTTGAA CGTCCAATCA 
AGCAAACAAC AGCTTCCAGC CATTAACTTC GATAGTGCCC AAAATAGCAT GACGAAGTCT 
GAGCCCGCCA TCAGGGCGGG TGGACACAGA GCTCGGGGTC AGTGGCATGA ATCCACAGAA 
GCTGTTGAAC TTGAAAATTT TAGTATAAAC TACAAGAATG AGAGAAATTT CAGCAAACAT 
CCTCAGCGTA AACTATTTCA GGAGATCTTT ACCGCCTTGG TGAAAAATAG ACTCATAAGC 
AGAGAGTGGG TTAATCGAGC CCCATCTATT CATTTTCTGA GAGTGTTAAT CTGTCTGAGG 
CTACTAATGA GGGATCCATG TTATCAGGAA ATACTCCATA GCTTGGGTGG GATTGAAAAC 
CTAGCTCAGT ATATGGAGAT TGTAGCCAAT GAGTACCTCG GCTATGGAGA AGAGCAGCAC 
ACTGTGGACA AGCTGGTCAA CATGACATAT ATTTTTCAAA AACTTGCTGC AGTCAAAGAT 
CAAAGAGAAT GGGTCACCAC AAGTGGAGCC CACAAGACAT TAGTAAATTT ACTTGGTGCC 
CGAGATACTA ATGTTCTATT GGGTTCCCTT CTGGCTCTGG CTAGTTTAGC AGAAAGTCAA 
GAATGTAGGG AGAAGATAAG TGAACTCAAC ATTGTAGAAA ATCTGTTGAT GATTTTACAT 
GAATATGACT TGCTTTCTAA AAGACTAACA GCGGAGTTGC TGCGCCTACT TTGTGCAGAG 
CCCCAGGTGA AAGAGCAGGT GAAGCTCTAT GAGGGGATAC CGGTCCTCCT CAGTCTGCTC 
CACTCTGACC ACTTGAAGCT CCTCTGGAGC ATTGTCTGGA TTCTGGTACA GGTTTGTGAG 
GACCCTGAGA CCAGCGTGGA AATTCGCATT TGGGGAGGCA TCAAACAGCT TCTTCATATT 
TTACAAGGAG ACAGAAATTT TGTTTCTGAT CACTCCTCCA TTGGAAGCCT GTCCAGTGCA 
AATGCTGCAG GCCGAATCCA GCAGCTTCAT TTATCAGAAG ACTTGAGCCC TAGGGAAATA 
CAAGAAAATA CTTTCTCACT TCAAGCAGCC TGCTGTGCTG CCCTCACTGA GCTGGTGCTC 
AATGACACCA ATGCCCACCA GGTGGTTCAG GAAAATGGTG TATATACAAT AGCAAAATTA 
ATTTTACCAA ATAAGCAAAA GAATGCAGCA AAAAGTAATC TATTACAGTG TTATGCTTTC 
AGAGCCTTGA GATTTCTCTT CAGTATGGAA AGAAACAGAC CACTCTTTAA AAGACTTTTC 
CCCACAGACT TGTTTGAGAT CTTCATTGAC ATAGGGCATT ATGTACGTGA TATCAGTGCT 
TATGAAGAAT TGGTATCCAA GCTGAATTTA TTAGTGGAGG ATGAACTGAA GCAAATTGCT 
GAAAATATTG AAAGCATTAA TCAGAACAAA GCTCCTTTGA AATATATAGG CAACTATGCA 
ATTTTGGATC ATCTTGGAAG TGGAGCTTTT GGCTGTGTTT ACAAGGTTAG AAAGCATAGT 
GGTCAAAATC TTTTAGCAAT GAAAGAGGTC AATTTACATA ACCCAGCATT TGGAAAGGAT 
AAGAAAGATC GAGACAGCAG CGTAAGGAAT ATTGTTTCTG AATTAACAAT AATTAAAGAG 
CAGCTTTATC ATCCCAACAT TGTACGTTAT TACAAAACAT TTCTGGAAAA TGATAGGTTG 
TACATAGTTA TGGAGCTGAT AGAAGGAGCC CCGCTTGGAG AGCATTTCAG TTCTTTGAAG 
GAAAAACATC ACCATTTTAC TGAAGAAAGA CTATGGAAAA TATTTATACA GCTGTGCTTA 
GCTCTTCGAT ACTTACACAA GGAGAAGAGG ATTGTCCATA GAGATCTGAC ACCAAACAAC 
ATTATGTTGG GGGATAAGGA CAAAGTAACC GTTACTGACT TTGGCCTGGC AAAGCAAAAA 
CAAGAAAACA GTAAACTCAC CTCTGTGGTT GGAACAATCC TGTATTCTTG CCCCGAGGTA 
CTGAAGAGTG AGCCGTATGG GGAGAAGGCT GATGTCTGGG CAGTAGGCTG CATCCTTTAT 
CAGATGGCGA CTTTGAGTCC CCCCTTCTAC AGCACTAACA TGCTGTCCTT GGCTACAAAA 
ATAGTGGAGG CGGTATATGA ACCAGTCCCA GAAGGTATCT ACTCTGAAAA AGTAACAGAC 
ACCATCAGCA GGTGCCTCAC TCCTGATGCG GAAGCTCGTC CAGATATTGT AGAAGTCAGT 
TCGATGATAT CAGATGTCAT GATGAAATAT TTAGACAACT TATCTACATC CCAGTTGTCC 
TTGGAAAAGA AGCTAGAACG GGAACGAAGA CGCACACAAA GGTATTTTAT GGAAGCCAAC 
CGGAACACCG TCACATGTCA CCATGAGCTG GCTGTTCTAT CTCACGAGAC CTTTGAGAAG 
GCAAGTTTGA GTAGCAGCAG CAGTGGAGCA GCCAGCCTGA AAAGTGAACT TTCAGAAAGC 
GCAGACCTGC CCCCTGAAGG CTTCCAGGCC TCCTATGGTA AAGACGAAGA CAGGGCCTGT 
GACGAAATCC TGTCAGATGA TAACTTCAAC CTGGAAAATG CTGAGAAAGA TACATATTCA 
GAGGTAGATG ATGAATTGGA CATTTCGGAT AACTCCAGCA GCTCCAGTTC AAGCCCTCTG 
AAAGAATCTA CATTCAACAT TTTAAAGAGA AGTTTTAGTG CTTCAGGAGG AGAAAGACAA 
TCCCAAACAA GGGACTTCAC TGGAGGAACA GGATCAAGAC CAAGACCAGC TTTGCTGCCT 
CTTGACCTGC TTCTGAAAGT GCCACCCCAC ATGCTCAGGG CCCACATTAA GGAAATAGAG 
GCTGAGTTAG TGACAGGGTG GCAGTCCCAT AGCCTTCCTG CTGTGATTCT TCGAAATCTC 
AAAGATCATG GGCCACAGAT GGGCACATTC TTGTGGCAAG CATCAGCAGG AATTGCTGTG 
TCCCAGAGGA AAGTGCGTCA GATCAGTGAT CCTATTCAGC AGATATTAAT TCAGCTGCAC 
AAAATAATCT ATATCACACA GCTTCCTCCA GCTTTGCACC ACAATTTGAA AAGAAGGGTT 
ATAGAGAGAT TCAAGAAATC CCTCTTCAGC CAGCAGAGTA ACCCTTGTAA TTTGAAATCT 
GAAATTAAAA AGTTATCTCA GGGATCTCCA GAACCGATTG AGCCCAACTT TTTCACAGCA 
GATTACCATT TATTACATCG TTCATCCGGT GGAAACAGCC TGTCCCCAAA TGACCCTACA 
GGTTTACCAA CCAGCATTGA ATTGGAGGAA GGAATAACAT ATGAACAGAT GCAGACTGTG 
ATTGAAGAAG TCCTTGAGGA AAGTGGCTAT TACAATTTTA CATCTAACAG GTATCATTCC 
TATCCATGGG GGACCAAGAA TCACCCAACC AAAAGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NEK10 ( XM_017005765.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016861254.1 Homo sapiens NIMA related kinase 10 (NEK10), transcript variant X6, mRNA.

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CloneID	OHu36991
Clone ID Related Accession (Same CDS sequence)	XM_017005764.1 , XM_017005762.2 , NM_152534.4 , XM_017005761.1 , XM_017005763.1 , XM_017005762.1 , XM_017005761.2 , XM_017005765.1
Accession Version	XM_017005765.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3519bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	serine/threonine-protein kinase Nek10 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000003.12) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCTGATC AAGATAAAAA GGTGAAGACC ACAGAAAAAT CAACTGATAA ACAGCAAGAA 
ATCACCATCA GGGACTATTC AGATCTTAAA AGACTTCGGT GCCTTTTGAA CGTCCAATCA 
AGCAAACAAC AGCTTCCAGC CATTAACTTC GATAGTGCCC AAAATAGCAT GACGAAGTCT 
GAGCCCGCCA TCAGGGCGGG TGGACACAGA GCTCGGGGTC AGTGGCATGA ATCCACAGAA 
GCTGTTGAAC TTGAAAATTT TAGTATAAAC TACAAGAATG AGAGAAATTT CAGCAAACAT 
CCTCAGCGTA AACTATTTCA GGAGATCTTT ACCGCCTTGG TGAAAAATAG ACTCATAAGC 
AGAGAGTGGG TTAATCGAGC CCCATCTATT CATTTTCTGA GAGTGTTAAT CTGTCTGAGG 
CTACTAATGA GGGATCCATG TTATCAGGAA ATACTCCATA GCTTGGGTGG GATTGAAAAC 
CTAGCTCAGT ATATGGAGAT TGTAGCCAAT GAGTACCTCG GCTATGGAGA AGAGCAGCAC 
ACTGTGGACA AGCTGGTCAA CATGACATAT ATTTTTCAAA AACTTGCTGC AGTCAAAGAT 
CAAAGAGAAT GGGTCACCAC AAGTGGAGCC CACAAGACAT TAGTAAATTT ACTTGGTGCC 
CGAGATACTA ATGTTCTATT GGGTTCCCTT CTGGCTCTGG CTAGTTTAGC AGAAAGTCAA 
GAATGTAGGG AGAAGATAAG TGAACTCAAC ATTGTAGAAA ATCTGTTGAT GATTTTACAT 
GAATATGACT TGCTTTCTAA AAGACTAACA GCGGAGTTGC TGCGCCTACT TTGTGCAGAG 
CCCCAGGTGA AAGAGCAGGT GAAGCTCTAT GAGGGGATAC CGGTCCTCCT CAGTCTGCTC 
CACTCTGACC ACTTGAAGCT CCTCTGGAGC ATTGTCTGGA TTCTGGTACA GGTTTGTGAG 
GACCCTGAGA CCAGCGTGGA AATTCGCATT TGGGGAGGCA TCAAACAGCT TCTTCATATT 
TTACAAGGAG ACAGAAATTT TGTTTCTGAT CACTCCTCCA TTGGAAGCCT GTCCAGTGCA 
AATGCTGCAG GCCGAATCCA GCAGCTTCAT TTATCAGAAG ACTTGAGCCC TAGGGAAATA 
CAAGAAAATA CTTTCTCACT TCAAGCAGCC TGCTGTGCTG CCCTCACTGA GCTGGTGCTC 
AATGACACCA ATGCCCACCA GGTGGTTCAG GAAAATGGTG TATATACAAT AGCAAAATTA 
ATTTTACCAA ATAAGCAAAA GAATGCAGCA AAAAGTAATC TATTACAGTG TTATGCTTTC 
AGAGCCTTGA GATTTCTCTT CAGTATGGAA AGAAACAGAC CACTCTTTAA AAGACTTTTC 
CCCACAGACT TGTTTGAGAT CTTCATTGAC ATAGGGCATT ATGTACGTGA TATCAGTGCT 
TATGAAGAAT TGGTATCCAA GCTGAATTTA TTAGTGGAGG ATGAACTGAA GCAAATTGCT 
GAAAATATTG AAAGCATTAA TCAGAACAAA GCTCCTTTGA AATATATAGG CAACTATGCA 
ATTTTGGATC ATCTTGGAAG TGGAGCTTTT GGCTGTGTTT ACAAGGTTAG AAAGCATAGT 
GGTCAAAATC TTTTAGCAAT GAAAGAGGTC AATTTACATA ACCCAGCATT TGGAAAGGAT 
AAGAAAGATC GAGACAGCAG CGTAAGGAAT ATTGTTTCTG AATTAACAAT AATTAAAGAG 
CAGCTTTATC ATCCCAACAT TGTACGTTAT TACAAAACAT TTCTGGAAAA TGATAGGTTG 
TACATAGTTA TGGAGCTGAT AGAAGGAGCC CCGCTTGGAG AGCATTTCAG TTCTTTGAAG 
GAAAAACATC ACCATTTTAC TGAAGAAAGA CTATGGAAAA TATTTATACA GCTGTGCTTA 
GCTCTTCGAT ACTTACACAA GGAGAAGAGG ATTGTCCATA GAGATCTGAC ACCAAACAAC 
ATTATGTTGG GGGATAAGGA CAAAGTAACC GTTACTGACT TTGGCCTGGC AAAGCAAAAA 
CAAGAAAACA GTAAACTCAC CTCTGTGGTT GGAACAATCC TGTATTCTTG CCCCGAGGTA 
CTGAAGAGTG AGCCGTATGG GGAGAAGGCT GATGTCTGGG CAGTAGGCTG CATCCTTTAT 
CAGATGGCGA CTTTGAGTCC CCCCTTCTAC AGCACTAACA TGCTGTCCTT GGCTACAAAA 
ATAGTGGAGG CGGTATATGA ACCAGTCCCA GAAGGTATCT ACTCTGAAAA AGTAACAGAC 
ACCATCAGCA GGTGCCTCAC TCCTGATGCG GAAGCTCGTC CAGATATTGT AGAAGTCAGT 
TCGATGATAT CAGATGTCAT GATGAAATAT TTAGACAACT TATCTACATC CCAGTTGTCC 
TTGGAAAAGA AGCTAGAACG GGAACGAAGA CGCACACAAA GGTATTTTAT GGAAGCCAAC 
CGGAACACCG TCACATGTCA CCATGAGCTG GCTGTTCTAT CTCACGAGAC CTTTGAGAAG 
GCAAGTTTGA GTAGCAGCAG CAGTGGAGCA GCCAGCCTGA AAAGTGAACT TTCAGAAAGC 
GCAGACCTGC CCCCTGAAGG CTTCCAGGCC TCCTATGGTA AAGACGAAGA CAGGGCCTGT 
GACGAAATCC TGTCAGATGA TAACTTCAAC CTGGAAAATG CTGAGAAAGA TACATATTCA 
GAGGTAGATG ATGAATTGGA CATTTCGGAT AACTCCAGCA GCTCCAGTTC AAGCCCTCTG 
AAAGAATCTA CATTCAACAT TTTAAAGAGA AGTTTTAGTG CTTCAGGAGG AGAAAGACAA 
TCCCAAACAA GGGACTTCAC TGGAGGAACA GGATCAAGAC CAAGACCAGC TTTGCTGCCT 
CTTGACCTGC TTCTGAAAGT GCCACCCCAC ATGCTCAGGG CCCACATTAA GGAAATAGAG 
GCTGAGTTAG TGACAGGGTG GCAGTCCCAT AGCCTTCCTG CTGTGATTCT TCGAAATCTC 
AAAGATCATG GGCCACAGAT GGGCACATTC TTGTGGCAAG CATCAGCAGG AATTGCTGTG 
TCCCAGAGGA AAGTGCGTCA GATCAGTGAT CCTATTCAGC AGATATTAAT TCAGCTGCAC 
AAAATAATCT ATATCACACA GCTTCCTCCA GCTTTGCACC ACAATTTGAA AAGAAGGGTT 
ATAGAGAGAT TCAAGAAATC CCTCTTCAGC CAGCAGAGTA ACCCTTGTAA TTTGAAATCT 
GAAATTAAAA AGTTATCTCA GGGATCTCCA GAACCGATTG AGCCCAACTT TTTCACAGCA 
GATTACCATT TATTACATCG TTCATCCGGT GGAAACAGCC TGTCCCCAAA TGACCCTACA 
GGTTTACCAA CCAGCATTGA ATTGGAGGAA GGAATAACAT ATGAACAGAT GCAGACTGTG 
ATTGAAGAAG TCCTTGAGGA AAGTGGCTAT TACAATTTTA CATCTAACAG GTATCATTCC 
TATCCATGGG GGACCAAGAA TCACCCAACC AAAAGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016861254.1
CDS	176..3694
Translation	MPDQDKKVKTTEKSTDKQQEITIRDYSDLKRLRCLLNVQSSKQQLPAINFDSAQNSMTKSEPAIRAGGHRARGQWHESTEAVELENFSINYKNERNFSKHPQRKLFQEIFTALVKNRLISREWVNRAPSIHFLRVLICLRLLMRDPCYQEILHSLGGIENLAQYMEIVANEYLGYGEEQHTVDKLVNMTYIFQKLAAVKDQREWVTTSGAHKTLVNLLGARDTNVLLGSLLALASLAESQECREKISELNIVENLLMILHEYDLLSKRLTAELLRLLCAEPQVKEQVKLYEGIPVLLSLLHSDHLKLLWSIVWILVQVCEDPETSVEIRIWGGIKQLLHILQGDRNFVSDHSSIGSLSSANAAGRIQQLHLSEDLSPREIQENTFSLQAACCAALTELVLNDTNAHQVVQENGVYTIAKLILPNKQKNAAKSNLLQCYAFRALRFLFSMERNRPLFKRLFPTDLFEIFIDIGHYVRDISAYEELVSKLNLLVEDELKQIAENIESINQNKAPLKYIGNYAILDHLGSGAFGCVYKVRKHSGQNLLAMKEVNLHNPAFGKDKKDRDSSVRNIVSELTIIKEQLYHPNIVRYYKTFLENDRLYIVMELIEGAPLGEHFSSLKEKHHHFTEERLWKIFIQLCLALRYLHKEKRIVHRDLTPNNIMLGDKDKVTVTDFGLAKQKQENSKLTSVVGTILYSCPEVLKSEPYGEKADVWAVGCILYQMATLSPPFYSTNMLSLATKIVEAVYEPVPEGIYSEKVTDTISRCLTPDAEARPDIVEVSSMISDVMMKYLDNLSTSQLSLEKKLERERRRTQRYFMEANRNTVTCHHELAVLSHETFEKASLSSSSSGAASLKSELSESADLPPEGFQASYGKDEDRACDEILSDDNFNLENAEKDTYSEVDDELDISDNSSSSSSSPLKESTFNILKRSFSASGGERQSQTRDFTGGTGSRPRPALLPLDLLLKVPPHMLRAHIKEIEAELVTGWQSHSLPAVILRNLKDHGPQMGTFLWQASAGIAVSQRKVRQISDPIQQILIQLHKIIYITQLPPALHHNLKRRVIERFKKSLFSQQSNPCNLKSEIKKLSQGSPEPIEPNFFTADYHLLHRSSGGNSLSPNDPTGLPTSIELEEGITYEQMQTVIEEVLEESGYYNFTSNRYHSYPWGTKNHPTKR

Target ORF information:

RefSeq Version	XM_017005765.1
Organism	Homo sapiens(human)
Definition	Homo sapiens NIMA related kinase 10 (NEK10), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017005765.1

ORF Insert Sequence:

ATGCCTGATC AAGATAAAAA GGTGAAGACC ACAGAAAAAT CAACTGATAA ACAGCAAGAA 
ATCACCATCA GGGACTATTC AGATCTTAAA AGACTTCGGT GCCTTTTGAA CGTCCAATCA 
AGCAAACAAC AGCTTCCAGC CATTAACTTC GATAGTGCCC AAAATAGCAT GACGAAGTCT 
GAGCCCGCCA TCAGGGCGGG TGGACACAGA GCTCGGGGTC AGTGGCATGA ATCCACAGAA 
GCTGTTGAAC TTGAAAATTT TAGTATAAAC TACAAGAATG AGAGAAATTT CAGCAAACAT 
CCTCAGCGTA AACTATTTCA GGAGATCTTT ACCGCCTTGG TGAAAAATAG ACTCATAAGC 
AGAGAGTGGG TTAATCGAGC CCCATCTATT CATTTTCTGA GAGTGTTAAT CTGTCTGAGG 
CTACTAATGA GGGATCCATG TTATCAGGAA ATACTCCATA GCTTGGGTGG GATTGAAAAC 
CTAGCTCAGT ATATGGAGAT TGTAGCCAAT GAGTACCTCG GCTATGGAGA AGAGCAGCAC 
ACTGTGGACA AGCTGGTCAA CATGACATAT ATTTTTCAAA AACTTGCTGC AGTCAAAGAT 
CAAAGAGAAT GGGTCACCAC AAGTGGAGCC CACAAGACAT TAGTAAATTT ACTTGGTGCC 
CGAGATACTA ATGTTCTATT GGGTTCCCTT CTGGCTCTGG CTAGTTTAGC AGAAAGTCAA 
GAATGTAGGG AGAAGATAAG TGAACTCAAC ATTGTAGAAA ATCTGTTGAT GATTTTACAT 
GAATATGACT TGCTTTCTAA AAGACTAACA GCGGAGTTGC TGCGCCTACT TTGTGCAGAG 
CCCCAGGTGA AAGAGCAGGT GAAGCTCTAT GAGGGGATAC CGGTCCTCCT CAGTCTGCTC 
CACTCTGACC ACTTGAAGCT CCTCTGGAGC ATTGTCTGGA TTCTGGTACA GGTTTGTGAG 
GACCCTGAGA CCAGCGTGGA AATTCGCATT TGGGGAGGCA TCAAACAGCT TCTTCATATT 
TTACAAGGAG ACAGAAATTT TGTTTCTGAT CACTCCTCCA TTGGAAGCCT GTCCAGTGCA 
AATGCTGCAG GCCGAATCCA GCAGCTTCAT TTATCAGAAG ACTTGAGCCC TAGGGAAATA 
CAAGAAAATA CTTTCTCACT TCAAGCAGCC TGCTGTGCTG CCCTCACTGA GCTGGTGCTC 
AATGACACCA ATGCCCACCA GGTGGTTCAG GAAAATGGTG TATATACAAT AGCAAAATTA 
ATTTTACCAA ATAAGCAAAA GAATGCAGCA AAAAGTAATC TATTACAGTG TTATGCTTTC 
AGAGCCTTGA GATTTCTCTT CAGTATGGAA AGAAACAGAC CACTCTTTAA AAGACTTTTC 
CCCACAGACT TGTTTGAGAT CTTCATTGAC ATAGGGCATT ATGTACGTGA TATCAGTGCT 
TATGAAGAAT TGGTATCCAA GCTGAATTTA TTAGTGGAGG ATGAACTGAA GCAAATTGCT 
GAAAATATTG AAAGCATTAA TCAGAACAAA GCTCCTTTGA AATATATAGG CAACTATGCA 
ATTTTGGATC ATCTTGGAAG TGGAGCTTTT GGCTGTGTTT ACAAGGTTAG AAAGCATAGT 
GGTCAAAATC TTTTAGCAAT GAAAGAGGTC AATTTACATA ACCCAGCATT TGGAAAGGAT 
AAGAAAGATC GAGACAGCAG CGTAAGGAAT ATTGTTTCTG AATTAACAAT AATTAAAGAG 
CAGCTTTATC ATCCCAACAT TGTACGTTAT TACAAAACAT TTCTGGAAAA TGATAGGTTG 
TACATAGTTA TGGAGCTGAT AGAAGGAGCC CCGCTTGGAG AGCATTTCAG TTCTTTGAAG 
GAAAAACATC ACCATTTTAC TGAAGAAAGA CTATGGAAAA TATTTATACA GCTGTGCTTA 
GCTCTTCGAT ACTTACACAA GGAGAAGAGG ATTGTCCATA GAGATCTGAC ACCAAACAAC 
ATTATGTTGG GGGATAAGGA CAAAGTAACC GTTACTGACT TTGGCCTGGC AAAGCAAAAA 
CAAGAAAACA GTAAACTCAC CTCTGTGGTT GGAACAATCC TGTATTCTTG CCCCGAGGTA 
CTGAAGAGTG AGCCGTATGG GGAGAAGGCT GATGTCTGGG CAGTAGGCTG CATCCTTTAT 
CAGATGGCGA CTTTGAGTCC CCCCTTCTAC AGCACTAACA TGCTGTCCTT GGCTACAAAA 
ATAGTGGAGG CGGTATATGA ACCAGTCCCA GAAGGTATCT ACTCTGAAAA AGTAACAGAC 
ACCATCAGCA GGTGCCTCAC TCCTGATGCG GAAGCTCGTC CAGATATTGT AGAAGTCAGT 
TCGATGATAT CAGATGTCAT GATGAAATAT TTAGACAACT TATCTACATC CCAGTTGTCC 
TTGGAAAAGA AGCTAGAACG GGAACGAAGA CGCACACAAA GGTATTTTAT GGAAGCCAAC 
CGGAACACCG TCACATGTCA CCATGAGCTG GCTGTTCTAT CTCACGAGAC CTTTGAGAAG 
GCAAGTTTGA GTAGCAGCAG CAGTGGAGCA GCCAGCCTGA AAAGTGAACT TTCAGAAAGC 
GCAGACCTGC CCCCTGAAGG CTTCCAGGCC TCCTATGGTA AAGACGAAGA CAGGGCCTGT 
GACGAAATCC TGTCAGATGA TAACTTCAAC CTGGAAAATG CTGAGAAAGA TACATATTCA 
GAGGTAGATG ATGAATTGGA CATTTCGGAT AACTCCAGCA GCTCCAGTTC AAGCCCTCTG 
AAAGAATCTA CATTCAACAT TTTAAAGAGA AGTTTTAGTG CTTCAGGAGG AGAAAGACAA 
TCCCAAACAA GGGACTTCAC TGGAGGAACA GGATCAAGAC CAAGACCAGC TTTGCTGCCT 
CTTGACCTGC TTCTGAAAGT GCCACCCCAC ATGCTCAGGG CCCACATTAA GGAAATAGAG 
GCTGAGTTAG TGACAGGGTG GCAGTCCCAT AGCCTTCCTG CTGTGATTCT TCGAAATCTC 
AAAGATCATG GGCCACAGAT GGGCACATTC TTGTGGCAAG CATCAGCAGG AATTGCTGTG 
TCCCAGAGGA AAGTGCGTCA GATCAGTGAT CCTATTCAGC AGATATTAAT TCAGCTGCAC 
AAAATAATCT ATATCACACA GCTTCCTCCA GCTTTGCACC ACAATTTGAA AAGAAGGGTT 
ATAGAGAGAT TCAAGAAATC CCTCTTCAGC CAGCAGAGTA ACCCTTGTAA TTTGAAATCT 
GAAATTAAAA AGTTATCTCA GGGATCTCCA GAACCGATTG AGCCCAACTT TTTCACAGCA 
GATTACCATT TATTACATCG TTCATCCGGT GGAAACAGCC TGTCCCCAAA TGACCCTACA 
GGTTTACCAA CCAGCATTGA ATTGGAGGAA GGAATAACAT ATGAACAGAT GCAGACTGTG 
ATTGAAGAAG TCCTTGAGGA AAGTGGCTAT TACAATTTTA CATCTAACAG GTATCATTCC 
TATCCATGGG GGACCAAGAA TCACCCAACC AAAAGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NEK10 ( XM_017005766.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016861255.1 Homo sapiens NIMA related kinase 10 (NEK10), transcript variant X8, mRNA.

Price & Availability↑

CloneID	OHu36992
Clone ID Related Accession (Same CDS sequence)	XM_017005766.1 , XM_017005766.2 , XM_006712997.3
Accession Version	XM_017005766.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3489bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	serine/threonine-protein kinase Nek10 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000003.12) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCTGATC AAGATAAAAA GGTGAAGACC ACAGAAAAAT CAACTGATAA ACAGCAAGAA 
ATCACCATCA GGGACTATTC AGATCTTAAA AGACTTCGGT GCCTTTTGAA CGTCCAATCA 
AGCAAACAAC AGCTTCCAGC CATTAACTTC GATAGTGCCC AAAATAGCAT GACGAAGTCT 
GAGCCCGCCA TCAGGGCGGG TGGACACAGA GCTCGGGGTC AGTGGCATGA ATCCACAGAA 
GCTGTTGAAC TTGAAAATTT TAGTATAAAC TACAAGAATG AGAGAAATTT CAGCAAACAT 
CCTCAGCGTA AACTATTTCA GGAGATCTTT ACCGCCTTGG TGAAAAATAG ACTCATAAGC 
AGAGAGTGGG TTAATCGAGC CCCATCTATT CATTTTCTGA GAGTGTTAAT CTGTCTGAGG 
CTACTAATGA GGGATCCATG TTATCAGGAA ATACTCCATA GCTTGGGTGG GATTGAAAAC 
CTAGCTCAGT ATATGGAGAT TGTAGCCAAT GAGTACCTCG GCTATGGAGA AGAGCAGCAC 
ACTGTGGACA AGCTGGTCAA CATGACATAT ATTTTTCAAA AACTTGCTGC AGTCAAAGAT 
CAAAGAGAAT GGGTCACCAC AAGTGGAGCC CACAAGACAT TAGTAAATTT ACTTGGTGCC 
CGAGATACTA ATGTTCTATT GGGTTCCCTT CTGGCTCTGG CTAGTTTAGC AGAAAGTCAA 
GAATGTAGGG AGAAGATAAG TGAACTCAAC ATTGTAGAAA ATCTGTTGAT GATTTTACAT 
GAATATGACT TGCTTTCTAA AAGACTAACA GCGGAGTTGC TGCGCCTACT TTGTGCAGAG 
CCCCAGGTGA AAGAGCAGGT GAAGCTCTAT GAGGGGATAC CGGTCCTCCT CAGTCTGCTC 
CACTCTGACC ACTTGAAGCT CCTCTGGAGC ATTGTCTGGA TTCTGGTACA GGTTTGTGAG 
GACCCTGAGA CCAGCGTGGA AATTCGCATT TGGGGAGGCA TCAAACAGCT TCTTCATATT 
TTACAAGGAG ACAGAAATTT TGTTTCTGAT CACTCCTCCA TTGGAAGCCT GTCCAGTGCA 
AATGCTGCAG GCCGAATCCA GCAGCTTCAT TTATCAGAAG ACTTGAGCCC TAGGGAAATA 
CAAGAAAATA CTTTCTCACT TCAAGCAGCC TGCTGTGCTG CCCTCACTGA GCTGGTGCTC 
AATGACACCA ATGCCCACCA GGTGGTTCAG GAAAATGGTG TATATACAAT AGCAAAATTA 
ATTTTACCAA ATAAGCAAAA GAATGCAGCA AAAAGTAATC TATTACAGTG TTATGCTTTC 
AGAGCCTTGA GATTTCTCTT CAGTATGGAA AGAAACAGAC CACTCTTTAA AAGACTTTTC 
CCCACAGACT TGTTTGAGAT CTTCATTGAC ATAGGGCATT ATGTACGTGA TATCAGTGCT 
TATGAAGAAT TGGTATCCAA GCTGAATTTA TTAGTGGAGG ATGAACTGAA GCAAATTGCT 
GAAAATATTG AAAGCATTAA TCAGAACAAA GCTCCTTTGA AATATATAGG CAACTATGCA 
ATTTTGGATC ATCTTGGAAG TGGAGCTTTT GGCTGTGTTT ACAAGGTTAG AAAGCATAGT 
GGTCAAAATC TTTTAGCAAT GAAAGAGGTC AATTTACATA ACCCAGCATT TGGAAAGGAT 
AAGAAAGATC GAGACAGCAG CGTAAGGAAT ATTGTTTCTG AATTAACAAT AATTAAAGAG 
CAGCTTTATC ATCCCAACAT TGTACGTTAT TACAAAACAT TTCTGGAAAA TGATAGGTTG 
TACATAGTTA TGGAGCTGAT AGAAGGAGCC CCGCTTGGAG AGCATTTCAG TTCTTTGAAG 
GAAAAACATC ACCATTTTAC TGAAGAAAGA CTATGGAAAA TATTTATACA GCTGTGCTTA 
GCTCTTCGAT ACTTACACAA GGAGAAGAGG ATTGTCCATA GAGATCTGAC ACCAAACAAC 
ATTATGTTGG GGGATAAGGA CAAAGTAACC GTTACTGACT TTGGCCTGGC AAAGCAAAAA 
CAAGAAAACA GTAAACTCAC CTCTGTGGTT GGAACAATCC TGTATTCTTG CCCCGAGGTA 
CTGAAGAGTG AGCCGTATGG GGAGAAGGCT GATGTCTGGG CAGTAGGCTG CATCCTTTAT 
CAGATGGCGA CTTTGAGTCC CCCCTTCTAC AGCACTAACA TGCTGTCCTT GGCTACAAAA 
ATAGTGGAGG CGGTATATGA ACCAGTCCCA GAAGGTATCT ACTCTGAAAA AGTAACAGAC 
ACCATCAGCA GGTGCCTCAC TCCTGATGCG GAAGCTCGTC CAGATATTGT AGAAGTCAGT 
TCGATGATAT CAGATGTCAT GATGAAATAT TTAGACAACT TATCTACATC CCAGTTGTCC 
TTGGAAAAGA AGCTAGAACG GGAACGAAGA CGCACACAAA GGTATTTTAT GGAAGCCAAC 
CGGAACACCG TCACATGTCA CCATGAGCTG GCTGTTCTAT CTCACGAGAC CTTTGAGAAG 
GCAAGTTTGA GTAGCAGCAG CAGTGGAGCA GCCAGCCTGA AAAGTGAACT TTCAGAAAGC 
GCAGACCTGC CCCCTGAAGG CTTCCAGGCC TCCTATGGTA AAGACGAAGA CAGGGCCTGT 
GACGAAATCC TGTCAGATGA TAACTTCAAC CTGGAAAATG CTGAGAAAGA TACATATTCA 
GAGGTAGATG ATGAATTGGA CATTTCGGAT AACTCCAGCA GCTCCAGTTC AAGCCCTCTG 
AAAGAATCTA CATTCAACAT TTTAAAGAGA AGTTTTAGTG CTTCAGGAGG AGAAAGACAA 
TCCCAAACAA GGGACTTCAC TGGAGGAACA GGATCAAGAC CAAGACCAGC TTTGCTGCCT 
CTTGACCTGC TTCTGAAAGT GCCACCCCAC ATGCTCAGGG CCCACATTAA GGAAATAGAG 
GCTGAGTTAG TGACAGGGTG GCAGTCCCAT AGCCTTCCTG CTGTGATTCT TCGAAATCTC 
AAAGATCATG CATCAGCAGG AATTGCTGTG TCCCAGAGGA AAGTGCGTCA GATCAGTGAT 
CCTATTCAGC AGATATTAAT TCAGCTGCAC AAAATAATCT ATATCACACA GCTTCCTCCA 
GCTTTGCACC ACAATTTGAA AAGAAGGGTT ATAGAGAGAT TCAAGAAATC CCTCTTCAGC 
CAGCAGAGTA ACCCTTGTAA TTTGAAATCT GAAATTAAAA AGTTATCTCA GGGATCTCCA 
GAACCGATTG AGCCCAACTT TTTCACAGCA GATTACCATT TATTACATCG TTCATCCGGT 
GGAAACAGCC TGTCCCCAAA TGACCCTACA GGTTTACCAA CCAGCATTGA ATTGGAGGAA 
GGAATAACAT ATGAACAGAT GCAGACTGTG ATTGAAGAAG TCCTTGAGGA AAGTGGCTAT 
TACAATTTTA CATCTAACAG GTATCATTCC TATCCATGGG GGACCAAGAA TCACCCAACC 
AAAAGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016861255.1
CDS	227..3715
Translation	MPDQDKKVKTTEKSTDKQQEITIRDYSDLKRLRCLLNVQSSKQQLPAINFDSAQNSMTKSEPAIRAGGHRARGQWHESTEAVELENFSINYKNERNFSKHPQRKLFQEIFTALVKNRLISREWVNRAPSIHFLRVLICLRLLMRDPCYQEILHSLGGIENLAQYMEIVANEYLGYGEEQHTVDKLVNMTYIFQKLAAVKDQREWVTTSGAHKTLVNLLGARDTNVLLGSLLALASLAESQECREKISELNIVENLLMILHEYDLLSKRLTAELLRLLCAEPQVKEQVKLYEGIPVLLSLLHSDHLKLLWSIVWILVQVCEDPETSVEIRIWGGIKQLLHILQGDRNFVSDHSSIGSLSSANAAGRIQQLHLSEDLSPREIQENTFSLQAACCAALTELVLNDTNAHQVVQENGVYTIAKLILPNKQKNAAKSNLLQCYAFRALRFLFSMERNRPLFKRLFPTDLFEIFIDIGHYVRDISAYEELVSKLNLLVEDELKQIAENIESINQNKAPLKYIGNYAILDHLGSGAFGCVYKVRKHSGQNLLAMKEVNLHNPAFGKDKKDRDSSVRNIVSELTIIKEQLYHPNIVRYYKTFLENDRLYIVMELIEGAPLGEHFSSLKEKHHHFTEERLWKIFIQLCLALRYLHKEKRIVHRDLTPNNIMLGDKDKVTVTDFGLAKQKQENSKLTSVVGTILYSCPEVLKSEPYGEKADVWAVGCILYQMATLSPPFYSTNMLSLATKIVEAVYEPVPEGIYSEKVTDTISRCLTPDAEARPDIVEVSSMISDVMMKYLDNLSTSQLSLEKKLERERRRTQRYFMEANRNTVTCHHELAVLSHETFEKASLSSSSSGAASLKSELSESADLPPEGFQASYGKDEDRACDEILSDDNFNLENAEKDTYSEVDDELDISDNSSSSSSSPLKESTFNILKRSFSASGGERQSQTRDFTGGTGSRPRPALLPLDLLLKVPPHMLRAHIKEIEAELVTGWQSHSLPAVILRNLKDHASAGIAVSQRKVRQISDPIQQILIQLHKIIYITQLPPALHHNLKRRVIERFKKSLFSQQSNPCNLKSEIKKLSQGSPEPIEPNFFTADYHLLHRSSGGNSLSPNDPTGLPTSIELEEGITYEQMQTVIEEVLEESGYYNFTSNRYHSYPWGTKNHPTKR

Target ORF information:

RefSeq Version	XM_017005766.1
Organism	Homo sapiens(human)
Definition	Homo sapiens NIMA related kinase 10 (NEK10), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017005766.1

ORF Insert Sequence:

ATGCCTGATC AAGATAAAAA GGTGAAGACC ACAGAAAAAT CAACTGATAA ACAGCAAGAA 
ATCACCATCA GGGACTATTC AGATCTTAAA AGACTTCGGT GCCTTTTGAA CGTCCAATCA 
AGCAAACAAC AGCTTCCAGC CATTAACTTC GATAGTGCCC AAAATAGCAT GACGAAGTCT 
GAGCCCGCCA TCAGGGCGGG TGGACACAGA GCTCGGGGTC AGTGGCATGA ATCCACAGAA 
GCTGTTGAAC TTGAAAATTT TAGTATAAAC TACAAGAATG AGAGAAATTT CAGCAAACAT 
CCTCAGCGTA AACTATTTCA GGAGATCTTT ACCGCCTTGG TGAAAAATAG ACTCATAAGC 
AGAGAGTGGG TTAATCGAGC CCCATCTATT CATTTTCTGA GAGTGTTAAT CTGTCTGAGG 
CTACTAATGA GGGATCCATG TTATCAGGAA ATACTCCATA GCTTGGGTGG GATTGAAAAC 
CTAGCTCAGT ATATGGAGAT TGTAGCCAAT GAGTACCTCG GCTATGGAGA AGAGCAGCAC 
ACTGTGGACA AGCTGGTCAA CATGACATAT ATTTTTCAAA AACTTGCTGC AGTCAAAGAT 
CAAAGAGAAT GGGTCACCAC AAGTGGAGCC CACAAGACAT TAGTAAATTT ACTTGGTGCC 
CGAGATACTA ATGTTCTATT GGGTTCCCTT CTGGCTCTGG CTAGTTTAGC AGAAAGTCAA 
GAATGTAGGG AGAAGATAAG TGAACTCAAC ATTGTAGAAA ATCTGTTGAT GATTTTACAT 
GAATATGACT TGCTTTCTAA AAGACTAACA GCGGAGTTGC TGCGCCTACT TTGTGCAGAG 
CCCCAGGTGA AAGAGCAGGT GAAGCTCTAT GAGGGGATAC CGGTCCTCCT CAGTCTGCTC 
CACTCTGACC ACTTGAAGCT CCTCTGGAGC ATTGTCTGGA TTCTGGTACA GGTTTGTGAG 
GACCCTGAGA CCAGCGTGGA AATTCGCATT TGGGGAGGCA TCAAACAGCT TCTTCATATT 
TTACAAGGAG ACAGAAATTT TGTTTCTGAT CACTCCTCCA TTGGAAGCCT GTCCAGTGCA 
AATGCTGCAG GCCGAATCCA GCAGCTTCAT TTATCAGAAG ACTTGAGCCC TAGGGAAATA 
CAAGAAAATA CTTTCTCACT TCAAGCAGCC TGCTGTGCTG CCCTCACTGA GCTGGTGCTC 
AATGACACCA ATGCCCACCA GGTGGTTCAG GAAAATGGTG TATATACAAT AGCAAAATTA 
ATTTTACCAA ATAAGCAAAA GAATGCAGCA AAAAGTAATC TATTACAGTG TTATGCTTTC 
AGAGCCTTGA GATTTCTCTT CAGTATGGAA AGAAACAGAC CACTCTTTAA AAGACTTTTC 
CCCACAGACT TGTTTGAGAT CTTCATTGAC ATAGGGCATT ATGTACGTGA TATCAGTGCT 
TATGAAGAAT TGGTATCCAA GCTGAATTTA TTAGTGGAGG ATGAACTGAA GCAAATTGCT 
GAAAATATTG AAAGCATTAA TCAGAACAAA GCTCCTTTGA AATATATAGG CAACTATGCA 
ATTTTGGATC ATCTTGGAAG TGGAGCTTTT GGCTGTGTTT ACAAGGTTAG AAAGCATAGT 
GGTCAAAATC TTTTAGCAAT GAAAGAGGTC AATTTACATA ACCCAGCATT TGGAAAGGAT 
AAGAAAGATC GAGACAGCAG CGTAAGGAAT ATTGTTTCTG AATTAACAAT AATTAAAGAG 
CAGCTTTATC ATCCCAACAT TGTACGTTAT TACAAAACAT TTCTGGAAAA TGATAGGTTG 
TACATAGTTA TGGAGCTGAT AGAAGGAGCC CCGCTTGGAG AGCATTTCAG TTCTTTGAAG 
GAAAAACATC ACCATTTTAC TGAAGAAAGA CTATGGAAAA TATTTATACA GCTGTGCTTA 
GCTCTTCGAT ACTTACACAA GGAGAAGAGG ATTGTCCATA GAGATCTGAC ACCAAACAAC 
ATTATGTTGG GGGATAAGGA CAAAGTAACC GTTACTGACT TTGGCCTGGC AAAGCAAAAA 
CAAGAAAACA GTAAACTCAC CTCTGTGGTT GGAACAATCC TGTATTCTTG CCCCGAGGTA 
CTGAAGAGTG AGCCGTATGG GGAGAAGGCT GATGTCTGGG CAGTAGGCTG CATCCTTTAT 
CAGATGGCGA CTTTGAGTCC CCCCTTCTAC AGCACTAACA TGCTGTCCTT GGCTACAAAA 
ATAGTGGAGG CGGTATATGA ACCAGTCCCA GAAGGTATCT ACTCTGAAAA AGTAACAGAC 
ACCATCAGCA GGTGCCTCAC TCCTGATGCG GAAGCTCGTC CAGATATTGT AGAAGTCAGT 
TCGATGATAT CAGATGTCAT GATGAAATAT TTAGACAACT TATCTACATC CCAGTTGTCC 
TTGGAAAAGA AGCTAGAACG GGAACGAAGA CGCACACAAA GGTATTTTAT GGAAGCCAAC 
CGGAACACCG TCACATGTCA CCATGAGCTG GCTGTTCTAT CTCACGAGAC CTTTGAGAAG 
GCAAGTTTGA GTAGCAGCAG CAGTGGAGCA GCCAGCCTGA AAAGTGAACT TTCAGAAAGC 
GCAGACCTGC CCCCTGAAGG CTTCCAGGCC TCCTATGGTA AAGACGAAGA CAGGGCCTGT 
GACGAAATCC TGTCAGATGA TAACTTCAAC CTGGAAAATG CTGAGAAAGA TACATATTCA 
GAGGTAGATG ATGAATTGGA CATTTCGGAT AACTCCAGCA GCTCCAGTTC AAGCCCTCTG 
AAAGAATCTA CATTCAACAT TTTAAAGAGA AGTTTTAGTG CTTCAGGAGG AGAAAGACAA 
TCCCAAACAA GGGACTTCAC TGGAGGAACA GGATCAAGAC CAAGACCAGC TTTGCTGCCT 
CTTGACCTGC TTCTGAAAGT GCCACCCCAC ATGCTCAGGG CCCACATTAA GGAAATAGAG 
GCTGAGTTAG TGACAGGGTG GCAGTCCCAT AGCCTTCCTG CTGTGATTCT TCGAAATCTC 
AAAGATCATG CATCAGCAGG AATTGCTGTG TCCCAGAGGA AAGTGCGTCA GATCAGTGAT 
CCTATTCAGC AGATATTAAT TCAGCTGCAC AAAATAATCT ATATCACACA GCTTCCTCCA 
GCTTTGCACC ACAATTTGAA AAGAAGGGTT ATAGAGAGAT TCAAGAAATC CCTCTTCAGC 
CAGCAGAGTA ACCCTTGTAA TTTGAAATCT GAAATTAAAA AGTTATCTCA GGGATCTCCA 
GAACCGATTG AGCCCAACTT TTTCACAGCA GATTACCATT TATTACATCG TTCATCCGGT 
GGAAACAGCC TGTCCCCAAA TGACCCTACA GGTTTACCAA CCAGCATTGA ATTGGAGGAA 
GGAATAACAT ATGAACAGAT GCAGACTGTG ATTGAAGAAG TCCTTGAGGA AAGTGGCTAT 
TACAATTTTA CATCTAACAG GTATCATTCC TATCCATGGG GGACCAAGAA TCACCCAACC 
AAAAGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NEK10 ( XM_017005766.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_016861255.1 Homo sapiens NIMA related kinase 10 (NEK10), transcript variant X8, mRNA.

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CloneID	OHu36992
Clone ID Related Accession (Same CDS sequence)	XM_017005766.1 , XM_017005766.2 , XM_006712997.3
Accession Version	XM_017005766.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3489bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	serine/threonine-protein kinase Nek10 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000003.12) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_017005766.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCTGATC AAGATAAAAA GGTGAAGACC ACAGAAAAAT CAACTGATAA ACAGCAAGAA 
ATCACCATCA GGGACTATTC AGATCTTAAA AGACTTCGGT GCCTTTTGAA CGTCCAATCA 
AGCAAACAAC AGCTTCCAGC CATTAACTTC GATAGTGCCC AAAATAGCAT GACGAAGTCT 
GAGCCCGCCA TCAGGGCGGG TGGACACAGA GCTCGGGGTC AGTGGCATGA ATCCACAGAA 
GCTGTTGAAC TTGAAAATTT TAGTATAAAC TACAAGAATG AGAGAAATTT CAGCAAACAT 
CCTCAGCGTA AACTATTTCA GGAGATCTTT ACCGCCTTGG TGAAAAATAG ACTCATAAGC 
AGAGAGTGGG TTAATCGAGC CCCATCTATT CATTTTCTGA GAGTGTTAAT CTGTCTGAGG 
CTACTAATGA GGGATCCATG TTATCAGGAA ATACTCCATA GCTTGGGTGG GATTGAAAAC 
CTAGCTCAGT ATATGGAGAT TGTAGCCAAT GAGTACCTCG GCTATGGAGA AGAGCAGCAC 
ACTGTGGACA AGCTGGTCAA CATGACATAT ATTTTTCAAA AACTTGCTGC AGTCAAAGAT 
CAAAGAGAAT GGGTCACCAC AAGTGGAGCC CACAAGACAT TAGTAAATTT ACTTGGTGCC 
CGAGATACTA ATGTTCTATT GGGTTCCCTT CTGGCTCTGG CTAGTTTAGC AGAAAGTCAA 
GAATGTAGGG AGAAGATAAG TGAACTCAAC ATTGTAGAAA ATCTGTTGAT GATTTTACAT 
GAATATGACT TGCTTTCTAA AAGACTAACA GCGGAGTTGC TGCGCCTACT TTGTGCAGAG 
CCCCAGGTGA AAGAGCAGGT GAAGCTCTAT GAGGGGATAC CGGTCCTCCT CAGTCTGCTC 
CACTCTGACC ACTTGAAGCT CCTCTGGAGC ATTGTCTGGA TTCTGGTACA GGTTTGTGAG 
GACCCTGAGA CCAGCGTGGA AATTCGCATT TGGGGAGGCA TCAAACAGCT TCTTCATATT 
TTACAAGGAG ACAGAAATTT TGTTTCTGAT CACTCCTCCA TTGGAAGCCT GTCCAGTGCA 
AATGCTGCAG GCCGAATCCA GCAGCTTCAT TTATCAGAAG ACTTGAGCCC TAGGGAAATA 
CAAGAAAATA CTTTCTCACT TCAAGCAGCC TGCTGTGCTG CCCTCACTGA GCTGGTGCTC 
AATGACACCA ATGCCCACCA GGTGGTTCAG GAAAATGGTG TATATACAAT AGCAAAATTA 
ATTTTACCAA ATAAGCAAAA GAATGCAGCA AAAAGTAATC TATTACAGTG TTATGCTTTC 
AGAGCCTTGA GATTTCTCTT CAGTATGGAA AGAAACAGAC CACTCTTTAA AAGACTTTTC 
CCCACAGACT TGTTTGAGAT CTTCATTGAC ATAGGGCATT ATGTACGTGA TATCAGTGCT 
TATGAAGAAT TGGTATCCAA GCTGAATTTA TTAGTGGAGG ATGAACTGAA GCAAATTGCT 
GAAAATATTG AAAGCATTAA TCAGAACAAA GCTCCTTTGA AATATATAGG CAACTATGCA 
ATTTTGGATC ATCTTGGAAG TGGAGCTTTT GGCTGTGTTT ACAAGGTTAG AAAGCATAGT 
GGTCAAAATC TTTTAGCAAT GAAAGAGGTC AATTTACATA ACCCAGCATT TGGAAAGGAT 
AAGAAAGATC GAGACAGCAG CGTAAGGAAT ATTGTTTCTG AATTAACAAT AATTAAAGAG 
CAGCTTTATC ATCCCAACAT TGTACGTTAT TACAAAACAT TTCTGGAAAA TGATAGGTTG 
TACATAGTTA TGGAGCTGAT AGAAGGAGCC CCGCTTGGAG AGCATTTCAG TTCTTTGAAG 
GAAAAACATC ACCATTTTAC TGAAGAAAGA CTATGGAAAA TATTTATACA GCTGTGCTTA 
GCTCTTCGAT ACTTACACAA GGAGAAGAGG ATTGTCCATA GAGATCTGAC ACCAAACAAC 
ATTATGTTGG GGGATAAGGA CAAAGTAACC GTTACTGACT TTGGCCTGGC AAAGCAAAAA 
CAAGAAAACA GTAAACTCAC CTCTGTGGTT GGAACAATCC TGTATTCTTG CCCCGAGGTA 
CTGAAGAGTG AGCCGTATGG GGAGAAGGCT GATGTCTGGG CAGTAGGCTG CATCCTTTAT 
CAGATGGCGA CTTTGAGTCC CCCCTTCTAC AGCACTAACA TGCTGTCCTT GGCTACAAAA 
ATAGTGGAGG CGGTATATGA ACCAGTCCCA GAAGGTATCT ACTCTGAAAA AGTAACAGAC 
ACCATCAGCA GGTGCCTCAC TCCTGATGCG GAAGCTCGTC CAGATATTGT AGAAGTCAGT 
TCGATGATAT CAGATGTCAT GATGAAATAT TTAGACAACT TATCTACATC CCAGTTGTCC 
TTGGAAAAGA AGCTAGAACG GGAACGAAGA CGCACACAAA GGTATTTTAT GGAAGCCAAC 
CGGAACACCG TCACATGTCA CCATGAGCTG GCTGTTCTAT CTCACGAGAC CTTTGAGAAG 
GCAAGTTTGA GTAGCAGCAG CAGTGGAGCA GCCAGCCTGA AAAGTGAACT TTCAGAAAGC 
GCAGACCTGC CCCCTGAAGG CTTCCAGGCC TCCTATGGTA AAGACGAAGA CAGGGCCTGT 
GACGAAATCC TGTCAGATGA TAACTTCAAC CTGGAAAATG CTGAGAAAGA TACATATTCA 
GAGGTAGATG ATGAATTGGA CATTTCGGAT AACTCCAGCA GCTCCAGTTC AAGCCCTCTG 
AAAGAATCTA CATTCAACAT TTTAAAGAGA AGTTTTAGTG CTTCAGGAGG AGAAAGACAA 
TCCCAAACAA GGGACTTCAC TGGAGGAACA GGATCAAGAC CAAGACCAGC TTTGCTGCCT 
CTTGACCTGC TTCTGAAAGT GCCACCCCAC ATGCTCAGGG CCCACATTAA GGAAATAGAG 
GCTGAGTTAG TGACAGGGTG GCAGTCCCAT AGCCTTCCTG CTGTGATTCT TCGAAATCTC 
AAAGATCATG CATCAGCAGG AATTGCTGTG TCCCAGAGGA AAGTGCGTCA GATCAGTGAT 
CCTATTCAGC AGATATTAAT TCAGCTGCAC AAAATAATCT ATATCACACA GCTTCCTCCA 
GCTTTGCACC ACAATTTGAA AAGAAGGGTT ATAGAGAGAT TCAAGAAATC CCTCTTCAGC 
CAGCAGAGTA ACCCTTGTAA TTTGAAATCT GAAATTAAAA AGTTATCTCA GGGATCTCCA 
GAACCGATTG AGCCCAACTT TTTCACAGCA GATTACCATT TATTACATCG TTCATCCGGT 
GGAAACAGCC TGTCCCCAAA TGACCCTACA GGTTTACCAA CCAGCATTGA ATTGGAGGAA 
GGAATAACAT ATGAACAGAT GCAGACTGTG ATTGAAGAAG TCCTTGAGGA AAGTGGCTAT 
TACAATTTTA CATCTAACAG GTATCATTCC TATCCATGGG GGACCAAGAA TCACCCAACC 
AAAAGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016861255.1
CDS	274..3762
Translation	MPDQDKKVKTTEKSTDKQQEITIRDYSDLKRLRCLLNVQSSKQQLPAINFDSAQNSMTKSEPAIRAGGHRARGQWHESTEAVELENFSINYKNERNFSKHPQRKLFQEIFTALVKNRLISREWVNRAPSIHFLRVLICLRLLMRDPCYQEILHSLGGIENLAQYMEIVANEYLGYGEEQHTVDKLVNMTYIFQKLAAVKDQREWVTTSGAHKTLVNLLGARDTNVLLGSLLALASLAESQECREKISELNIVENLLMILHEYDLLSKRLTAELLRLLCAEPQVKEQVKLYEGIPVLLSLLHSDHLKLLWSIVWILVQVCEDPETSVEIRIWGGIKQLLHILQGDRNFVSDHSSIGSLSSANAAGRIQQLHLSEDLSPREIQENTFSLQAACCAALTELVLNDTNAHQVVQENGVYTIAKLILPNKQKNAAKSNLLQCYAFRALRFLFSMERNRPLFKRLFPTDLFEIFIDIGHYVRDISAYEELVSKLNLLVEDELKQIAENIESINQNKAPLKYIGNYAILDHLGSGAFGCVYKVRKHSGQNLLAMKEVNLHNPAFGKDKKDRDSSVRNIVSELTIIKEQLYHPNIVRYYKTFLENDRLYIVMELIEGAPLGEHFSSLKEKHHHFTEERLWKIFIQLCLALRYLHKEKRIVHRDLTPNNIMLGDKDKVTVTDFGLAKQKQENSKLTSVVGTILYSCPEVLKSEPYGEKADVWAVGCILYQMATLSPPFYSTNMLSLATKIVEAVYEPVPEGIYSEKVTDTISRCLTPDAEARPDIVEVSSMISDVMMKYLDNLSTSQLSLEKKLERERRRTQRYFMEANRNTVTCHHELAVLSHETFEKASLSSSSSGAASLKSELSESADLPPEGFQASYGKDEDRACDEILSDDNFNLENAEKDTYSEVDDELDISDNSSSSSSSPLKESTFNILKRSFSASGGERQSQTRDFTGGTGSRPRPALLPLDLLLKVPPHMLRAHIKEIEAELVTGWQSHSLPAVILRNLKDHASAGIAVSQRKVRQISDPIQQILIQLHKIIYITQLPPALHHNLKRRVIERFKKSLFSQQSNPCNLKSEIKKLSQGSPEPIEPNFFTADYHLLHRSSGGNSLSPNDPTGLPTSIELEEGITYEQMQTVIEEVLEESGYYNFTSNRYHSYPWGTKNHPTKR

Target ORF information:

RefSeq Version	XM_017005766.2
Organism	Homo sapiens(human)
Definition	Homo sapiens NIMA related kinase 10 (NEK10), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017005766.2

ORF Insert Sequence:

ATGCCTGATC AAGATAAAAA GGTGAAGACC ACAGAAAAAT CAACTGATAA ACAGCAAGAA 
ATCACCATCA GGGACTATTC AGATCTTAAA AGACTTCGGT GCCTTTTGAA CGTCCAATCA 
AGCAAACAAC AGCTTCCAGC CATTAACTTC GATAGTGCCC AAAATAGCAT GACGAAGTCT 
GAGCCCGCCA TCAGGGCGGG TGGACACAGA GCTCGGGGTC AGTGGCATGA ATCCACAGAA 
GCTGTTGAAC TTGAAAATTT TAGTATAAAC TACAAGAATG AGAGAAATTT CAGCAAACAT 
CCTCAGCGTA AACTATTTCA GGAGATCTTT ACCGCCTTGG TGAAAAATAG ACTCATAAGC 
AGAGAGTGGG TTAATCGAGC CCCATCTATT CATTTTCTGA GAGTGTTAAT CTGTCTGAGG 
CTACTAATGA GGGATCCATG TTATCAGGAA ATACTCCATA GCTTGGGTGG GATTGAAAAC 
CTAGCTCAGT ATATGGAGAT TGTAGCCAAT GAGTACCTCG GCTATGGAGA AGAGCAGCAC 
ACTGTGGACA AGCTGGTCAA CATGACATAT ATTTTTCAAA AACTTGCTGC AGTCAAAGAT 
CAAAGAGAAT GGGTCACCAC AAGTGGAGCC CACAAGACAT TAGTAAATTT ACTTGGTGCC 
CGAGATACTA ATGTTCTATT GGGTTCCCTT CTGGCTCTGG CTAGTTTAGC AGAAAGTCAA 
GAATGTAGGG AGAAGATAAG TGAACTCAAC ATTGTAGAAA ATCTGTTGAT GATTTTACAT 
GAATATGACT TGCTTTCTAA AAGACTAACA GCGGAGTTGC TGCGCCTACT TTGTGCAGAG 
CCCCAGGTGA AAGAGCAGGT GAAGCTCTAT GAGGGGATAC CGGTCCTCCT CAGTCTGCTC 
CACTCTGACC ACTTGAAGCT CCTCTGGAGC ATTGTCTGGA TTCTGGTACA GGTTTGTGAG 
GACCCTGAGA CCAGCGTGGA AATTCGCATT TGGGGAGGCA TCAAACAGCT TCTTCATATT 
TTACAAGGAG ACAGAAATTT TGTTTCTGAT CACTCCTCCA TTGGAAGCCT GTCCAGTGCA 
AATGCTGCAG GCCGAATCCA GCAGCTTCAT TTATCAGAAG ACTTGAGCCC TAGGGAAATA 
CAAGAAAATA CTTTCTCACT TCAAGCAGCC TGCTGTGCTG CCCTCACTGA GCTGGTGCTC 
AATGACACCA ATGCCCACCA GGTGGTTCAG GAAAATGGTG TATATACAAT AGCAAAATTA 
ATTTTACCAA ATAAGCAAAA GAATGCAGCA AAAAGTAATC TATTACAGTG TTATGCTTTC 
AGAGCCTTGA GATTTCTCTT CAGTATGGAA AGAAACAGAC CACTCTTTAA AAGACTTTTC 
CCCACAGACT TGTTTGAGAT CTTCATTGAC ATAGGGCATT ATGTACGTGA TATCAGTGCT 
TATGAAGAAT TGGTATCCAA GCTGAATTTA TTAGTGGAGG ATGAACTGAA GCAAATTGCT 
GAAAATATTG AAAGCATTAA TCAGAACAAA GCTCCTTTGA AATATATAGG CAACTATGCA 
ATTTTGGATC ATCTTGGAAG TGGAGCTTTT GGCTGTGTTT ACAAGGTTAG AAAGCATAGT 
GGTCAAAATC TTTTAGCAAT GAAAGAGGTC AATTTACATA ACCCAGCATT TGGAAAGGAT 
AAGAAAGATC GAGACAGCAG CGTAAGGAAT ATTGTTTCTG AATTAACAAT AATTAAAGAG 
CAGCTTTATC ATCCCAACAT TGTACGTTAT TACAAAACAT TTCTGGAAAA TGATAGGTTG 
TACATAGTTA TGGAGCTGAT AGAAGGAGCC CCGCTTGGAG AGCATTTCAG TTCTTTGAAG 
GAAAAACATC ACCATTTTAC TGAAGAAAGA CTATGGAAAA TATTTATACA GCTGTGCTTA 
GCTCTTCGAT ACTTACACAA GGAGAAGAGG ATTGTCCATA GAGATCTGAC ACCAAACAAC 
ATTATGTTGG GGGATAAGGA CAAAGTAACC GTTACTGACT TTGGCCTGGC AAAGCAAAAA 
CAAGAAAACA GTAAACTCAC CTCTGTGGTT GGAACAATCC TGTATTCTTG CCCCGAGGTA 
CTGAAGAGTG AGCCGTATGG GGAGAAGGCT GATGTCTGGG CAGTAGGCTG CATCCTTTAT 
CAGATGGCGA CTTTGAGTCC CCCCTTCTAC AGCACTAACA TGCTGTCCTT GGCTACAAAA 
ATAGTGGAGG CGGTATATGA ACCAGTCCCA GAAGGTATCT ACTCTGAAAA AGTAACAGAC 
ACCATCAGCA GGTGCCTCAC TCCTGATGCG GAAGCTCGTC CAGATATTGT AGAAGTCAGT 
TCGATGATAT CAGATGTCAT GATGAAATAT TTAGACAACT TATCTACATC CCAGTTGTCC 
TTGGAAAAGA AGCTAGAACG GGAACGAAGA CGCACACAAA GGTATTTTAT GGAAGCCAAC 
CGGAACACCG TCACATGTCA CCATGAGCTG GCTGTTCTAT CTCACGAGAC CTTTGAGAAG 
GCAAGTTTGA GTAGCAGCAG CAGTGGAGCA GCCAGCCTGA AAAGTGAACT TTCAGAAAGC 
GCAGACCTGC CCCCTGAAGG CTTCCAGGCC TCCTATGGTA AAGACGAAGA CAGGGCCTGT 
GACGAAATCC TGTCAGATGA TAACTTCAAC CTGGAAAATG CTGAGAAAGA TACATATTCA 
GAGGTAGATG ATGAATTGGA CATTTCGGAT AACTCCAGCA GCTCCAGTTC AAGCCCTCTG 
AAAGAATCTA CATTCAACAT TTTAAAGAGA AGTTTTAGTG CTTCAGGAGG AGAAAGACAA 
TCCCAAACAA GGGACTTCAC TGGAGGAACA GGATCAAGAC CAAGACCAGC TTTGCTGCCT 
CTTGACCTGC TTCTGAAAGT GCCACCCCAC ATGCTCAGGG CCCACATTAA GGAAATAGAG 
GCTGAGTTAG TGACAGGGTG GCAGTCCCAT AGCCTTCCTG CTGTGATTCT TCGAAATCTC 
AAAGATCATG CATCAGCAGG AATTGCTGTG TCCCAGAGGA AAGTGCGTCA GATCAGTGAT 
CCTATTCAGC AGATATTAAT TCAGCTGCAC AAAATAATCT ATATCACACA GCTTCCTCCA 
GCTTTGCACC ACAATTTGAA AAGAAGGGTT ATAGAGAGAT TCAAGAAATC CCTCTTCAGC 
CAGCAGAGTA ACCCTTGTAA TTTGAAATCT GAAATTAAAA AGTTATCTCA GGGATCTCCA 
GAACCGATTG AGCCCAACTT TTTCACAGCA GATTACCATT TATTACATCG TTCATCCGGT 
GGAAACAGCC TGTCCCCAAA TGACCCTACA GGTTTACCAA CCAGCATTGA ATTGGAGGAA 
GGAATAACAT ATGAACAGAT GCAGACTGTG ATTGAAGAAG TCCTTGAGGA AAGTGGCTAT 
TACAATTTTA CATCTAACAG GTATCATTCC TATCCATGGG GGACCAAGAA TCACCCAACC 
AAAAGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NEK10 ( XM_006712997.3 ) cDNA ORF clone, Homo sapiens(human) -> XP_006713060.1 Homo sapiens NIMA related kinase 10 (NEK10), transcript variant X7, mRNA.

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CloneID	OHu36992
Clone ID Related Accession (Same CDS sequence)	XM_017005766.1 , XM_017005766.2 , XM_006712997.3
Accession Version	XM_006712997.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3489bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	serine/threonine-protein kinase Nek10 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000003.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_006712997.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCTGATC AAGATAAAAA GGTGAAGACC ACAGAAAAAT CAACTGATAA ACAGCAAGAA 
ATCACCATCA GGGACTATTC AGATCTTAAA AGACTTCGGT GCCTTTTGAA CGTCCAATCA 
AGCAAACAAC AGCTTCCAGC CATTAACTTC GATAGTGCCC AAAATAGCAT GACGAAGTCT 
GAGCCCGCCA TCAGGGCGGG TGGACACAGA GCTCGGGGTC AGTGGCATGA ATCCACAGAA 
GCTGTTGAAC TTGAAAATTT TAGTATAAAC TACAAGAATG AGAGAAATTT CAGCAAACAT 
CCTCAGCGTA AACTATTTCA GGAGATCTTT ACCGCCTTGG TGAAAAATAG ACTCATAAGC 
AGAGAGTGGG TTAATCGAGC CCCATCTATT CATTTTCTGA GAGTGTTAAT CTGTCTGAGG 
CTACTAATGA GGGATCCATG TTATCAGGAA ATACTCCATA GCTTGGGTGG GATTGAAAAC 
CTAGCTCAGT ATATGGAGAT TGTAGCCAAT GAGTACCTCG GCTATGGAGA AGAGCAGCAC 
ACTGTGGACA AGCTGGTCAA CATGACATAT ATTTTTCAAA AACTTGCTGC AGTCAAAGAT 
CAAAGAGAAT GGGTCACCAC AAGTGGAGCC CACAAGACAT TAGTAAATTT ACTTGGTGCC 
CGAGATACTA ATGTTCTATT GGGTTCCCTT CTGGCTCTGG CTAGTTTAGC AGAAAGTCAA 
GAATGTAGGG AGAAGATAAG TGAACTCAAC ATTGTAGAAA ATCTGTTGAT GATTTTACAT 
GAATATGACT TGCTTTCTAA AAGACTAACA GCGGAGTTGC TGCGCCTACT TTGTGCAGAG 
CCCCAGGTGA AAGAGCAGGT GAAGCTCTAT GAGGGGATAC CGGTCCTCCT CAGTCTGCTC 
CACTCTGACC ACTTGAAGCT CCTCTGGAGC ATTGTCTGGA TTCTGGTACA GGTTTGTGAG 
GACCCTGAGA CCAGCGTGGA AATTCGCATT TGGGGAGGCA TCAAACAGCT TCTTCATATT 
TTACAAGGAG ACAGAAATTT TGTTTCTGAT CACTCCTCCA TTGGAAGCCT GTCCAGTGCA 
AATGCTGCAG GCCGAATCCA GCAGCTTCAT TTATCAGAAG ACTTGAGCCC TAGGGAAATA 
CAAGAAAATA CTTTCTCACT TCAAGCAGCC TGCTGTGCTG CCCTCACTGA GCTGGTGCTC 
AATGACACCA ATGCCCACCA GGTGGTTCAG GAAAATGGTG TATATACAAT AGCAAAATTA 
ATTTTACCAA ATAAGCAAAA GAATGCAGCA AAAAGTAATC TATTACAGTG TTATGCTTTC 
AGAGCCTTGA GATTTCTCTT CAGTATGGAA AGAAACAGAC CACTCTTTAA AAGACTTTTC 
CCCACAGACT TGTTTGAGAT CTTCATTGAC ATAGGGCATT ATGTACGTGA TATCAGTGCT 
TATGAAGAAT TGGTATCCAA GCTGAATTTA TTAGTGGAGG ATGAACTGAA GCAAATTGCT 
GAAAATATTG AAAGCATTAA TCAGAACAAA GCTCCTTTGA AATATATAGG CAACTATGCA 
ATTTTGGATC ATCTTGGAAG TGGAGCTTTT GGCTGTGTTT ACAAGGTTAG AAAGCATAGT 
GGTCAAAATC TTTTAGCAAT GAAAGAGGTC AATTTACATA ACCCAGCATT TGGAAAGGAT 
AAGAAAGATC GAGACAGCAG CGTAAGGAAT ATTGTTTCTG AATTAACAAT AATTAAAGAG 
CAGCTTTATC ATCCCAACAT TGTACGTTAT TACAAAACAT TTCTGGAAAA TGATAGGTTG 
TACATAGTTA TGGAGCTGAT AGAAGGAGCC CCGCTTGGAG AGCATTTCAG TTCTTTGAAG 
GAAAAACATC ACCATTTTAC TGAAGAAAGA CTATGGAAAA TATTTATACA GCTGTGCTTA 
GCTCTTCGAT ACTTACACAA GGAGAAGAGG ATTGTCCATA GAGATCTGAC ACCAAACAAC 
ATTATGTTGG GGGATAAGGA CAAAGTAACC GTTACTGACT TTGGCCTGGC AAAGCAAAAA 
CAAGAAAACA GTAAACTCAC CTCTGTGGTT GGAACAATCC TGTATTCTTG CCCCGAGGTA 
CTGAAGAGTG AGCCGTATGG GGAGAAGGCT GATGTCTGGG CAGTAGGCTG CATCCTTTAT 
CAGATGGCGA CTTTGAGTCC CCCCTTCTAC AGCACTAACA TGCTGTCCTT GGCTACAAAA 
ATAGTGGAGG CGGTATATGA ACCAGTCCCA GAAGGTATCT ACTCTGAAAA AGTAACAGAC 
ACCATCAGCA GGTGCCTCAC TCCTGATGCG GAAGCTCGTC CAGATATTGT AGAAGTCAGT 
TCGATGATAT CAGATGTCAT GATGAAATAT TTAGACAACT TATCTACATC CCAGTTGTCC 
TTGGAAAAGA AGCTAGAACG GGAACGAAGA CGCACACAAA GGTATTTTAT GGAAGCCAAC 
CGGAACACCG TCACATGTCA CCATGAGCTG GCTGTTCTAT CTCACGAGAC CTTTGAGAAG 
GCAAGTTTGA GTAGCAGCAG CAGTGGAGCA GCCAGCCTGA AAAGTGAACT TTCAGAAAGC 
GCAGACCTGC CCCCTGAAGG CTTCCAGGCC TCCTATGGTA AAGACGAAGA CAGGGCCTGT 
GACGAAATCC TGTCAGATGA TAACTTCAAC CTGGAAAATG CTGAGAAAGA TACATATTCA 
GAGGTAGATG ATGAATTGGA CATTTCGGAT AACTCCAGCA GCTCCAGTTC AAGCCCTCTG 
AAAGAATCTA CATTCAACAT TTTAAAGAGA AGTTTTAGTG CTTCAGGAGG AGAAAGACAA 
TCCCAAACAA GGGACTTCAC TGGAGGAACA GGATCAAGAC CAAGACCAGC TTTGCTGCCT 
CTTGACCTGC TTCTGAAAGT GCCACCCCAC ATGCTCAGGG CCCACATTAA GGAAATAGAG 
GCTGAGTTAG TGACAGGGTG GCAGTCCCAT AGCCTTCCTG CTGTGATTCT TCGAAATCTC 
AAAGATCATG CATCAGCAGG AATTGCTGTG TCCCAGAGGA AAGTGCGTCA GATCAGTGAT 
CCTATTCAGC AGATATTAAT TCAGCTGCAC AAAATAATCT ATATCACACA GCTTCCTCCA 
GCTTTGCACC ACAATTTGAA AAGAAGGGTT ATAGAGAGAT TCAAGAAATC CCTCTTCAGC 
CAGCAGAGTA ACCCTTGTAA TTTGAAATCT GAAATTAAAA AGTTATCTCA GGGATCTCCA 
GAACCGATTG AGCCCAACTT TTTCACAGCA GATTACCATT TATTACATCG TTCATCCGGT 
GGAAACAGCC TGTCCCCAAA TGACCCTACA GGTTTACCAA CCAGCATTGA ATTGGAGGAA 
GGAATAACAT ATGAACAGAT GCAGACTGTG ATTGAAGAAG TCCTTGAGGA AAGTGGCTAT 
TACAATTTTA CATCTAACAG GTATCATTCC TATCCATGGG GGACCAAGAA TCACCCAACC 
AAAAGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006713060.1
CDS	275..3763
Translation	MPDQDKKVKTTEKSTDKQQEITIRDYSDLKRLRCLLNVQSSKQQLPAINFDSAQNSMTKSEPAIRAGGHRARGQWHESTEAVELENFSINYKNERNFSKHPQRKLFQEIFTALVKNRLISREWVNRAPSIHFLRVLICLRLLMRDPCYQEILHSLGGIENLAQYMEIVANEYLGYGEEQHTVDKLVNMTYIFQKLAAVKDQREWVTTSGAHKTLVNLLGARDTNVLLGSLLALASLAESQECREKISELNIVENLLMILHEYDLLSKRLTAELLRLLCAEPQVKEQVKLYEGIPVLLSLLHSDHLKLLWSIVWILVQVCEDPETSVEIRIWGGIKQLLHILQGDRNFVSDHSSIGSLSSANAAGRIQQLHLSEDLSPREIQENTFSLQAACCAALTELVLNDTNAHQVVQENGVYTIAKLILPNKQKNAAKSNLLQCYAFRALRFLFSMERNRPLFKRLFPTDLFEIFIDIGHYVRDISAYEELVSKLNLLVEDELKQIAENIESINQNKAPLKYIGNYAILDHLGSGAFGCVYKVRKHSGQNLLAMKEVNLHNPAFGKDKKDRDSSVRNIVSELTIIKEQLYHPNIVRYYKTFLENDRLYIVMELIEGAPLGEHFSSLKEKHHHFTEERLWKIFIQLCLALRYLHKEKRIVHRDLTPNNIMLGDKDKVTVTDFGLAKQKQENSKLTSVVGTILYSCPEVLKSEPYGEKADVWAVGCILYQMATLSPPFYSTNMLSLATKIVEAVYEPVPEGIYSEKVTDTISRCLTPDAEARPDIVEVSSMISDVMMKYLDNLSTSQLSLEKKLERERRRTQRYFMEANRNTVTCHHELAVLSHETFEKASLSSSSSGAASLKSELSESADLPPEGFQASYGKDEDRACDEILSDDNFNLENAEKDTYSEVDDELDISDNSSSSSSSPLKESTFNILKRSFSASGGERQSQTRDFTGGTGSRPRPALLPLDLLLKVPPHMLRAHIKEIEAELVTGWQSHSLPAVILRNLKDHASAGIAVSQRKVRQISDPIQQILIQLHKIIYITQLPPALHHNLKRRVIERFKKSLFSQQSNPCNLKSEIKKLSQGSPEPIEPNFFTADYHLLHRSSGGNSLSPNDPTGLPTSIELEEGITYEQMQTVIEEVLEESGYYNFTSNRYHSYPWGTKNHPTKR

Target ORF information:

RefSeq Version	XM_006712997.3
Organism	Homo sapiens(human)
Definition	Homo sapiens NIMA related kinase 10 (NEK10), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006712997.3

ORF Insert Sequence:

ATGCCTGATC AAGATAAAAA GGTGAAGACC ACAGAAAAAT CAACTGATAA ACAGCAAGAA 
ATCACCATCA GGGACTATTC AGATCTTAAA AGACTTCGGT GCCTTTTGAA CGTCCAATCA 
AGCAAACAAC AGCTTCCAGC CATTAACTTC GATAGTGCCC AAAATAGCAT GACGAAGTCT 
GAGCCCGCCA TCAGGGCGGG TGGACACAGA GCTCGGGGTC AGTGGCATGA ATCCACAGAA 
GCTGTTGAAC TTGAAAATTT TAGTATAAAC TACAAGAATG AGAGAAATTT CAGCAAACAT 
CCTCAGCGTA AACTATTTCA GGAGATCTTT ACCGCCTTGG TGAAAAATAG ACTCATAAGC 
AGAGAGTGGG TTAATCGAGC CCCATCTATT CATTTTCTGA GAGTGTTAAT CTGTCTGAGG 
CTACTAATGA GGGATCCATG TTATCAGGAA ATACTCCATA GCTTGGGTGG GATTGAAAAC 
CTAGCTCAGT ATATGGAGAT TGTAGCCAAT GAGTACCTCG GCTATGGAGA AGAGCAGCAC 
ACTGTGGACA AGCTGGTCAA CATGACATAT ATTTTTCAAA AACTTGCTGC AGTCAAAGAT 
CAAAGAGAAT GGGTCACCAC AAGTGGAGCC CACAAGACAT TAGTAAATTT ACTTGGTGCC 
CGAGATACTA ATGTTCTATT GGGTTCCCTT CTGGCTCTGG CTAGTTTAGC AGAAAGTCAA 
GAATGTAGGG AGAAGATAAG TGAACTCAAC ATTGTAGAAA ATCTGTTGAT GATTTTACAT 
GAATATGACT TGCTTTCTAA AAGACTAACA GCGGAGTTGC TGCGCCTACT TTGTGCAGAG 
CCCCAGGTGA AAGAGCAGGT GAAGCTCTAT GAGGGGATAC CGGTCCTCCT CAGTCTGCTC 
CACTCTGACC ACTTGAAGCT CCTCTGGAGC ATTGTCTGGA TTCTGGTACA GGTTTGTGAG 
GACCCTGAGA CCAGCGTGGA AATTCGCATT TGGGGAGGCA TCAAACAGCT TCTTCATATT 
TTACAAGGAG ACAGAAATTT TGTTTCTGAT CACTCCTCCA TTGGAAGCCT GTCCAGTGCA 
AATGCTGCAG GCCGAATCCA GCAGCTTCAT TTATCAGAAG ACTTGAGCCC TAGGGAAATA 
CAAGAAAATA CTTTCTCACT TCAAGCAGCC TGCTGTGCTG CCCTCACTGA GCTGGTGCTC 
AATGACACCA ATGCCCACCA GGTGGTTCAG GAAAATGGTG TATATACAAT AGCAAAATTA 
ATTTTACCAA ATAAGCAAAA GAATGCAGCA AAAAGTAATC TATTACAGTG TTATGCTTTC 
AGAGCCTTGA GATTTCTCTT CAGTATGGAA AGAAACAGAC CACTCTTTAA AAGACTTTTC 
CCCACAGACT TGTTTGAGAT CTTCATTGAC ATAGGGCATT ATGTACGTGA TATCAGTGCT 
TATGAAGAAT TGGTATCCAA GCTGAATTTA TTAGTGGAGG ATGAACTGAA GCAAATTGCT 
GAAAATATTG AAAGCATTAA TCAGAACAAA GCTCCTTTGA AATATATAGG CAACTATGCA 
ATTTTGGATC ATCTTGGAAG TGGAGCTTTT GGCTGTGTTT ACAAGGTTAG AAAGCATAGT 
GGTCAAAATC TTTTAGCAAT GAAAGAGGTC AATTTACATA ACCCAGCATT TGGAAAGGAT 
AAGAAAGATC GAGACAGCAG CGTAAGGAAT ATTGTTTCTG AATTAACAAT AATTAAAGAG 
CAGCTTTATC ATCCCAACAT TGTACGTTAT TACAAAACAT TTCTGGAAAA TGATAGGTTG 
TACATAGTTA TGGAGCTGAT AGAAGGAGCC CCGCTTGGAG AGCATTTCAG TTCTTTGAAG 
GAAAAACATC ACCATTTTAC TGAAGAAAGA CTATGGAAAA TATTTATACA GCTGTGCTTA 
GCTCTTCGAT ACTTACACAA GGAGAAGAGG ATTGTCCATA GAGATCTGAC ACCAAACAAC 
ATTATGTTGG GGGATAAGGA CAAAGTAACC GTTACTGACT TTGGCCTGGC AAAGCAAAAA 
CAAGAAAACA GTAAACTCAC CTCTGTGGTT GGAACAATCC TGTATTCTTG CCCCGAGGTA 
CTGAAGAGTG AGCCGTATGG GGAGAAGGCT GATGTCTGGG CAGTAGGCTG CATCCTTTAT 
CAGATGGCGA CTTTGAGTCC CCCCTTCTAC AGCACTAACA TGCTGTCCTT GGCTACAAAA 
ATAGTGGAGG CGGTATATGA ACCAGTCCCA GAAGGTATCT ACTCTGAAAA AGTAACAGAC 
ACCATCAGCA GGTGCCTCAC TCCTGATGCG GAAGCTCGTC CAGATATTGT AGAAGTCAGT 
TCGATGATAT CAGATGTCAT GATGAAATAT TTAGACAACT TATCTACATC CCAGTTGTCC 
TTGGAAAAGA AGCTAGAACG GGAACGAAGA CGCACACAAA GGTATTTTAT GGAAGCCAAC 
CGGAACACCG TCACATGTCA CCATGAGCTG GCTGTTCTAT CTCACGAGAC CTTTGAGAAG 
GCAAGTTTGA GTAGCAGCAG CAGTGGAGCA GCCAGCCTGA AAAGTGAACT TTCAGAAAGC 
GCAGACCTGC CCCCTGAAGG CTTCCAGGCC TCCTATGGTA AAGACGAAGA CAGGGCCTGT 
GACGAAATCC TGTCAGATGA TAACTTCAAC CTGGAAAATG CTGAGAAAGA TACATATTCA 
GAGGTAGATG ATGAATTGGA CATTTCGGAT AACTCCAGCA GCTCCAGTTC AAGCCCTCTG 
AAAGAATCTA CATTCAACAT TTTAAAGAGA AGTTTTAGTG CTTCAGGAGG AGAAAGACAA 
TCCCAAACAA GGGACTTCAC TGGAGGAACA GGATCAAGAC CAAGACCAGC TTTGCTGCCT 
CTTGACCTGC TTCTGAAAGT GCCACCCCAC ATGCTCAGGG CCCACATTAA GGAAATAGAG 
GCTGAGTTAG TGACAGGGTG GCAGTCCCAT AGCCTTCCTG CTGTGATTCT TCGAAATCTC 
AAAGATCATG CATCAGCAGG AATTGCTGTG TCCCAGAGGA AAGTGCGTCA GATCAGTGAT 
CCTATTCAGC AGATATTAAT TCAGCTGCAC AAAATAATCT ATATCACACA GCTTCCTCCA 
GCTTTGCACC ACAATTTGAA AAGAAGGGTT ATAGAGAGAT TCAAGAAATC CCTCTTCAGC 
CAGCAGAGTA ACCCTTGTAA TTTGAAATCT GAAATTAAAA AGTTATCTCA GGGATCTCCA 
GAACCGATTG AGCCCAACTT TTTCACAGCA GATTACCATT TATTACATCG TTCATCCGGT 
GGAAACAGCC TGTCCCCAAA TGACCCTACA GGTTTACCAA CCAGCATTGA ATTGGAGGAA 
GGAATAACAT ATGAACAGAT GCAGACTGTG ATTGAAGAAG TCCTTGAGGA AAGTGGCTAT 
TACAATTTTA CATCTAACAG GTATCATTCC TATCCATGGG GGACCAAGAA TCACCCAACC 
AAAAGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NEK10 ( XM_006712998.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_006713061.1 Homo sapiens NIMA related kinase 10 (NEK10), transcript variant X9, mRNA.

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CloneID	OHu36993
Clone ID Related Accession (Same CDS sequence)	XM_006712998.2
Accession Version	XM_006712998.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3489bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	serine/threonine-protein kinase Nek10 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000003.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 12, 2015 this sequence version replaced XM_006712998.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCTGATC AAGATAAAAA GGTGAAGACC ACAGAAAAAT CAACTGATAA ACAGCAAGAA 
ATCACCATCA GGGACTATTC AGATCTTAAA AGACTTCGGT GCCTTTTGAA CGTCCAATCA 
AGCAAACAAC AGCTTCCAGC CATTAACTTC GATAGTGCCC AAAATAGCAT GACGAAGTCT 
GAGCCCGCCA TCAGGGCGGG TGGACACAGA GCTCGGGGTC AGTGGCATGA ATCCACAGAA 
GCTGTTGAAC TTGAAAATTT TAGTATAAAC TACAAGAATG AGAGAAATTT CAGCAAACAT 
CCTCAGCGTA AACTATTTCA GGAGATCTTT ACCGCCTTGG TGAAAAATAG ACTCATAAGC 
AGAGAGTGGG TTAATCGAGC CCCATCTATT CATTTTCTGA GAGTGTTAAT CTGTCTGAGG 
CTACTAATGA GGGATCCATG TTATCAGGAA ATACTCCATA GCTTGGGTGG GATTGAAAAC 
CTAGCTCAGT ATATGGAGAT TGTAGCCAAT GAGTACCTCG GCTATGGAGA AGAGCAGCAC 
ACTGTGGACA AGCTGGTCAA CATGACATAT ATTTTTCAAA AACTTGCTGC AGTCAAAGAT 
CAAAGAGAAT GGGTCACCAC AAGTGGAGCC CACAAGACAT TAGTAAATTT ACTTGGTGCC 
CGAGATACTA ATGTTCTATT GGGTTCCCTT CTGGCTCTGG CTAGTTTAGC AGAAAGTCAA 
GAATGTAGGG AGAAGATAAG TGAACTCAAC ATTGTAGAAA ATCTGTTGAT GATTTTACAT 
GAATATGACT TGCTTTCTAA AAGACTAACA GCGGAGTTGC TGCGCCTACT TTGTGCAGAG 
CCCCAGGTGA AAGAGCAGGT GAAGCTCTAT GAGGGGATAC CGGTCCTCCT CAGTCTGCTC 
CACTCTGACC ACTTGAAGCT CCTCTGGAGC ATTGTCTGGA TTCTGGTACA GGTTTGTGAG 
GACCCTGAGA CCAGCGTGGA AATTCGCATT TGGGGAGGCA TCAAACAGCT TCTTCATATT 
TTACAAGGAG ACAGAAATTT TGTTTCTGAT CACTCCTCCA TTGGAAGCCT GTCCAGTGCA 
AATGCTGCAG GCCGAATCCA GCAGCTTCAT TTATCAGAAG ACTTGAGCCC TAGGGAAATA 
CAAGAAAATA CTTTCTCACT TCAAGCAGCC TGCTGTGCTG CCCTCACTGA GCTGGTGCTC 
AATGACACCA ATGCCCACCA GGTGGTTCAG GAAAATGGTG TATATACAAT AGCAAAATTA 
ATTTTACCAA ATAAGCAAAA GAATGCAGCA AAAAGTAATC TATTACAGTG TTATGCTTTC 
AGAGCCTTGA GATTTCTCTT CAGTATGGAA AGAAACAGAC CACTCTTTAA AAGACTTTTC 
CCCACAGACT TGTTTGAGAT CTTCATTGAC ATAGGGCATT ATGTACGTGA TATCAGTGCT 
TATGAAGAAT TGGTATCCAA GCTGAATTTA TTAGTGGAGG ATGAACTGAA GCAAATTGCT 
GAAAATATTG AAAGCATTAA TCAGAACAAA GCTCCTTTGA AATATATAGG CAACTATGCA 
ATTTTGGATC ATCTTGGAAG TGGAGCTTTT GGCTGTGTTT ACAAGGTTAG AAAGCATAGT 
GGTCAAAATC TTTTAGCAAT GAAAGAGGTC AATTTACATA ACCCAGCATT TGGAAAGGAT 
AAGAAAGATC GAGACAGCAG CGTAAGGAAT ATTGTTTCTG AATTAACAAT AATTAAAGAG 
CAGCTTTATC ATCCCAACAT TGTACGTTAT TACAAAACAT TTCTGGAAAA TGATAGGTTG 
TACATAGTTA TGGAGCTGAT AGAAGGAGCC CCGCTTGGAG AGCATTTCAG TTCTTTGAAG 
GAAAAACATC ACCATTTTAC TGAAGAAAGA CTATGGAAAA TATTTATACA GCTGTGCTTA 
GCTCTTCGAT ACTTACACAA GGAGAAGAGG ATTGTCCATA GAGATCTGAC ACCAAACAAC 
ATTATGTTGG GGGATAAGGA CAAAGTAACC GTTACTGACT TTGGCCTGGC AAAGCAAAAA 
CAAGAAAACA GTAAACTCAC CTCTGTGGTT GGAACAATCC TGTATTCTTG CCCCGAGGTA 
CTGAAGAGTG AGCCGTATGG GGAGAAGGCT GATGTCTGGG CAGTAGGCTG CATCCTTTAT 
CAGATGGCGA CTTTGAGTCC CCCCTTCTAC AGCACTAACA TGCTGTCCTT GGCTACAAAA 
ATAGTGGAGG CGGTATATGA ACCAGTCCCA GAAGGTATCT ACTCTGAAAA AGTAACAGAC 
ACCATCAGCA GGTGCCTCAC TCCTGATGCG GAAGCTCGTC CAGATATTGT AGAAGTCAGT 
TCGATGATAT CAGATGTCAT GATGAAATAT TTAGACAACT TATCTACATC CCAGTTGTCC 
TTGGAAAAGA AGCTAGAACG GGAACGAAGA CGCACACAAA GGTATTTTAT GGAAGCCAAC 
CGGAACACCG TCACATGTCA CCATGAGCTG GCTGTTCTAT CTCACGAGAC CTTTGAGAAG 
GCAAGTTTGA GTAGCAGCAG CAGTGGAGCA GCCAGCCTGA AAAGTGAACT TTCAGAAAGC 
GCAGACCTGC CCCCTGAAGG CTTCCAGGCC TCCTATGGTA AAGACGAAGA CAGGGCCTGT 
GACGAAATCC TGTCAGATGA TAACTTCAAC CTGGAAAATG CTGAGAAAGA TACATATTCA 
GAGGTAGATG ATGAATTGGA CATTTCGGAT AACTCCAGCA GCTCCAGTTC AAGCCCTCTG 
AAAGAATCTA CATTCAACAT TTTAAAGAGA AGTTTTAGTG CTTCAGGAGG AGAAAGACAA 
TCCCAAACAA GGGACTTCAC TGGAGGAACA GGATCAAGAC CAAGACCAGC TTTGCTGCCT 
CTTGACCTGC TTCTGAAAGT GCCACCCCAC ATGCTCAGGG CCCACATTAA GGAAATAGAG 
GCTGAGTTAG TGACAGGGTG GCAGTCCCAT AGCCTTCCTG CTGTGATTCT TCGAAATCTC 
AAAGATCATG GGCCACAGAT GGGCACATTC TTGTGGCAAG CATCAGCAGG AATTGCTGTG 
TCCCAGAGGA AAGTGCGTCA GATCAGTGAT CCTATTCAGC AGATATTAAT TCAGCTGCAC 
AAAATAATCT ATATCACACA GCTTCCTCCA GCTTTGCACC ACAATTTGAA AAGAAGGGTT 
ATAGAGAGAT TCAAGAAATC CCTCTTCAGC CAGCAGAGTA ACCCTTGTAA TTTGAAATCT 
GAAATTAAAA AGTTATCTCA GGGATCTCCA GAACCGATTG AGCCCAACTT TTTCACAGCA 
GATTACCATT TATTACATCG TTCATCCGGT GGAAACAGCC TGTCCCCAAA TGACCCTACA 
GGTTTACCAA CCAGCATTGA ATTGGAGGAA GGAATAACAT ATGAACAGAT GCAGACTGTG 
ATTGAAGAAG TCCTTGAGGA AAGTGGCTAT TACAATTTTA CATCTAACAG GGCTGAGTAT 
ATGCATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006713061.1
CDS	275..3763
Translation	MPDQDKKVKTTEKSTDKQQEITIRDYSDLKRLRCLLNVQSSKQQLPAINFDSAQNSMTKSEPAIRAGGHRARGQWHESTEAVELENFSINYKNERNFSKHPQRKLFQEIFTALVKNRLISREWVNRAPSIHFLRVLICLRLLMRDPCYQEILHSLGGIENLAQYMEIVANEYLGYGEEQHTVDKLVNMTYIFQKLAAVKDQREWVTTSGAHKTLVNLLGARDTNVLLGSLLALASLAESQECREKISELNIVENLLMILHEYDLLSKRLTAELLRLLCAEPQVKEQVKLYEGIPVLLSLLHSDHLKLLWSIVWILVQVCEDPETSVEIRIWGGIKQLLHILQGDRNFVSDHSSIGSLSSANAAGRIQQLHLSEDLSPREIQENTFSLQAACCAALTELVLNDTNAHQVVQENGVYTIAKLILPNKQKNAAKSNLLQCYAFRALRFLFSMERNRPLFKRLFPTDLFEIFIDIGHYVRDISAYEELVSKLNLLVEDELKQIAENIESINQNKAPLKYIGNYAILDHLGSGAFGCVYKVRKHSGQNLLAMKEVNLHNPAFGKDKKDRDSSVRNIVSELTIIKEQLYHPNIVRYYKTFLENDRLYIVMELIEGAPLGEHFSSLKEKHHHFTEERLWKIFIQLCLALRYLHKEKRIVHRDLTPNNIMLGDKDKVTVTDFGLAKQKQENSKLTSVVGTILYSCPEVLKSEPYGEKADVWAVGCILYQMATLSPPFYSTNMLSLATKIVEAVYEPVPEGIYSEKVTDTISRCLTPDAEARPDIVEVSSMISDVMMKYLDNLSTSQLSLEKKLERERRRTQRYFMEANRNTVTCHHELAVLSHETFEKASLSSSSSGAASLKSELSESADLPPEGFQASYGKDEDRACDEILSDDNFNLENAEKDTYSEVDDELDISDNSSSSSSSPLKESTFNILKRSFSASGGERQSQTRDFTGGTGSRPRPALLPLDLLLKVPPHMLRAHIKEIEAELVTGWQSHSLPAVILRNLKDHGPQMGTFLWQASAGIAVSQRKVRQISDPIQQILIQLHKIIYITQLPPALHHNLKRRVIERFKKSLFSQQSNPCNLKSEIKKLSQGSPEPIEPNFFTADYHLLHRSSGGNSLSPNDPTGLPTSIELEEGITYEQMQTVIEEVLEESGYYNFTSNRAEYMH

Target ORF information:

RefSeq Version	XM_006712998.2
Organism	Homo sapiens(human)
Definition	Homo sapiens NIMA related kinase 10 (NEK10), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006712998.2

ORF Insert Sequence:

ATGCCTGATC AAGATAAAAA GGTGAAGACC ACAGAAAAAT CAACTGATAA ACAGCAAGAA 
ATCACCATCA GGGACTATTC AGATCTTAAA AGACTTCGGT GCCTTTTGAA CGTCCAATCA 
AGCAAACAAC AGCTTCCAGC CATTAACTTC GATAGTGCCC AAAATAGCAT GACGAAGTCT 
GAGCCCGCCA TCAGGGCGGG TGGACACAGA GCTCGGGGTC AGTGGCATGA ATCCACAGAA 
GCTGTTGAAC TTGAAAATTT TAGTATAAAC TACAAGAATG AGAGAAATTT CAGCAAACAT 
CCTCAGCGTA AACTATTTCA GGAGATCTTT ACCGCCTTGG TGAAAAATAG ACTCATAAGC 
AGAGAGTGGG TTAATCGAGC CCCATCTATT CATTTTCTGA GAGTGTTAAT CTGTCTGAGG 
CTACTAATGA GGGATCCATG TTATCAGGAA ATACTCCATA GCTTGGGTGG GATTGAAAAC 
CTAGCTCAGT ATATGGAGAT TGTAGCCAAT GAGTACCTCG GCTATGGAGA AGAGCAGCAC 
ACTGTGGACA AGCTGGTCAA CATGACATAT ATTTTTCAAA AACTTGCTGC AGTCAAAGAT 
CAAAGAGAAT GGGTCACCAC AAGTGGAGCC CACAAGACAT TAGTAAATTT ACTTGGTGCC 
CGAGATACTA ATGTTCTATT GGGTTCCCTT CTGGCTCTGG CTAGTTTAGC AGAAAGTCAA 
GAATGTAGGG AGAAGATAAG TGAACTCAAC ATTGTAGAAA ATCTGTTGAT GATTTTACAT 
GAATATGACT TGCTTTCTAA AAGACTAACA GCGGAGTTGC TGCGCCTACT TTGTGCAGAG 
CCCCAGGTGA AAGAGCAGGT GAAGCTCTAT GAGGGGATAC CGGTCCTCCT CAGTCTGCTC 
CACTCTGACC ACTTGAAGCT CCTCTGGAGC ATTGTCTGGA TTCTGGTACA GGTTTGTGAG 
GACCCTGAGA CCAGCGTGGA AATTCGCATT TGGGGAGGCA TCAAACAGCT TCTTCATATT 
TTACAAGGAG ACAGAAATTT TGTTTCTGAT CACTCCTCCA TTGGAAGCCT GTCCAGTGCA 
AATGCTGCAG GCCGAATCCA GCAGCTTCAT TTATCAGAAG ACTTGAGCCC TAGGGAAATA 
CAAGAAAATA CTTTCTCACT TCAAGCAGCC TGCTGTGCTG CCCTCACTGA GCTGGTGCTC 
AATGACACCA ATGCCCACCA GGTGGTTCAG GAAAATGGTG TATATACAAT AGCAAAATTA 
ATTTTACCAA ATAAGCAAAA GAATGCAGCA AAAAGTAATC TATTACAGTG TTATGCTTTC 
AGAGCCTTGA GATTTCTCTT CAGTATGGAA AGAAACAGAC CACTCTTTAA AAGACTTTTC 
CCCACAGACT TGTTTGAGAT CTTCATTGAC ATAGGGCATT ATGTACGTGA TATCAGTGCT 
TATGAAGAAT TGGTATCCAA GCTGAATTTA TTAGTGGAGG ATGAACTGAA GCAAATTGCT 
GAAAATATTG AAAGCATTAA TCAGAACAAA GCTCCTTTGA AATATATAGG CAACTATGCA 
ATTTTGGATC ATCTTGGAAG TGGAGCTTTT GGCTGTGTTT ACAAGGTTAG AAAGCATAGT 
GGTCAAAATC TTTTAGCAAT GAAAGAGGTC AATTTACATA ACCCAGCATT TGGAAAGGAT 
AAGAAAGATC GAGACAGCAG CGTAAGGAAT ATTGTTTCTG AATTAACAAT AATTAAAGAG 
CAGCTTTATC ATCCCAACAT TGTACGTTAT TACAAAACAT TTCTGGAAAA TGATAGGTTG 
TACATAGTTA TGGAGCTGAT AGAAGGAGCC CCGCTTGGAG AGCATTTCAG TTCTTTGAAG 
GAAAAACATC ACCATTTTAC TGAAGAAAGA CTATGGAAAA TATTTATACA GCTGTGCTTA 
GCTCTTCGAT ACTTACACAA GGAGAAGAGG ATTGTCCATA GAGATCTGAC ACCAAACAAC 
ATTATGTTGG GGGATAAGGA CAAAGTAACC GTTACTGACT TTGGCCTGGC AAAGCAAAAA 
CAAGAAAACA GTAAACTCAC CTCTGTGGTT GGAACAATCC TGTATTCTTG CCCCGAGGTA 
CTGAAGAGTG AGCCGTATGG GGAGAAGGCT GATGTCTGGG CAGTAGGCTG CATCCTTTAT 
CAGATGGCGA CTTTGAGTCC CCCCTTCTAC AGCACTAACA TGCTGTCCTT GGCTACAAAA 
ATAGTGGAGG CGGTATATGA ACCAGTCCCA GAAGGTATCT ACTCTGAAAA AGTAACAGAC 
ACCATCAGCA GGTGCCTCAC TCCTGATGCG GAAGCTCGTC CAGATATTGT AGAAGTCAGT 
TCGATGATAT CAGATGTCAT GATGAAATAT TTAGACAACT TATCTACATC CCAGTTGTCC 
TTGGAAAAGA AGCTAGAACG GGAACGAAGA CGCACACAAA GGTATTTTAT GGAAGCCAAC 
CGGAACACCG TCACATGTCA CCATGAGCTG GCTGTTCTAT CTCACGAGAC CTTTGAGAAG 
GCAAGTTTGA GTAGCAGCAG CAGTGGAGCA GCCAGCCTGA AAAGTGAACT TTCAGAAAGC 
GCAGACCTGC CCCCTGAAGG CTTCCAGGCC TCCTATGGTA AAGACGAAGA CAGGGCCTGT 
GACGAAATCC TGTCAGATGA TAACTTCAAC CTGGAAAATG CTGAGAAAGA TACATATTCA 
GAGGTAGATG ATGAATTGGA CATTTCGGAT AACTCCAGCA GCTCCAGTTC AAGCCCTCTG 
AAAGAATCTA CATTCAACAT TTTAAAGAGA AGTTTTAGTG CTTCAGGAGG AGAAAGACAA 
TCCCAAACAA GGGACTTCAC TGGAGGAACA GGATCAAGAC CAAGACCAGC TTTGCTGCCT 
CTTGACCTGC TTCTGAAAGT GCCACCCCAC ATGCTCAGGG CCCACATTAA GGAAATAGAG 
GCTGAGTTAG TGACAGGGTG GCAGTCCCAT AGCCTTCCTG CTGTGATTCT TCGAAATCTC 
AAAGATCATG GGCCACAGAT GGGCACATTC TTGTGGCAAG CATCAGCAGG AATTGCTGTG 
TCCCAGAGGA AAGTGCGTCA GATCAGTGAT CCTATTCAGC AGATATTAAT TCAGCTGCAC 
AAAATAATCT ATATCACACA GCTTCCTCCA GCTTTGCACC ACAATTTGAA AAGAAGGGTT 
ATAGAGAGAT TCAAGAAATC CCTCTTCAGC CAGCAGAGTA ACCCTTGTAA TTTGAAATCT 
GAAATTAAAA AGTTATCTCA GGGATCTCCA GAACCGATTG AGCCCAACTT TTTCACAGCA 
GATTACCATT TATTACATCG TTCATCCGGT GGAAACAGCC TGTCCCCAAA TGACCCTACA 
GGTTTACCAA CCAGCATTGA ATTGGAGGAA GGAATAACAT ATGAACAGAT GCAGACTGTG 
ATTGAAGAAG TCCTTGAGGA AAGTGGCTAT TACAATTTTA CATCTAACAG GGCTGAGTAT 
ATGCATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NEK10 ( XM_006712999.3 ) cDNA ORF clone, Homo sapiens(human) -> XP_006713062.1 Homo sapiens NIMA related kinase 10 (NEK10), transcript variant X10, mRNA.

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CloneID	OHu36994
Clone ID Related Accession (Same CDS sequence)	XM_006712999.3
Accession Version	XM_006712999.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3477bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	serine/threonine-protein kinase Nek10 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000003.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_006712999.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCTGATC AAGATAAAAA GGTGAAGACC ACAGAAAAAT CAACTGATAA ACAGCAAGAA 
ATCACCATCA GGGACTATTC AGATCTTAAA AGACTTCGGT GCCTTTTGAA CGTCCAATCA 
AGCAAACAAC AGCTTCCAGC CATTAACTTC GATAGTGCCC AAAATAGCAT GACGAAGTCT 
GAGCCCGCCA TCAGGGCGGG TGGACACAGA GCTCGGGGTC AGTGGCATGA ATCCACAGAA 
GCTGTTGAAC TTGAAAATTT TAGTATAAAC TACAAGAATG AGAGAAATTT CAGCAAACAT 
CCTCAGCGTA AACTATTTCA GGAGATCTTT ACCGCCTTGG TGAAAAATAG ACTCATAAGC 
AGAGAGTGGG TTAATCGAGC CCCATCTATT CATTTTCTGA GAGTGTTAAT CTGTCTGAGG 
CTACTAATGA GGGATCCATG TTATCAGGAA ATACTCCATA GCTTGGGTGG GATTGAAAAC 
CTAGCTCAGT ATATGGAGAT TGTAGCCAAT GAGTACCTCG GCTATGGAGA AGAGCAGCAC 
ACTGTGGACA AGCTGGTCAA CATGACATAT ATTTTTCAAA AACTTGCTGC AGTCAAAGAT 
CAAAGAGAAT GGGTCACCAC AAGTGGAGCC CACAAGACAT TAGTAAATTT ACTTGGTGCC 
CGAGATACTA ATGTTCTATT GGGTTCCCTT CTGGCTCTGG CTAGTTTAGC AGAAAGTCAA 
GAATGTAGGG AGAAGATAAG TGAACTCAAC ATTGTAGAAA ATCTGTTGAT GATTTTACAT 
GAATATGACT TGCTTTCTAA AAGACTAACA GCGGAGTTGC TGCGCCTACT TTGTGCAGAG 
CCCCAGGTGA AAGAGCAGGT GAAGCTCTAT GAGGGGATAC CGGTCCTCCT CAGTCTGCTC 
CACTCTGACC ACTTGAAGCT CCTCTGGAGC ATTGTCTGGA TTCTGGTACA GGTTTGTGAG 
GACCCTGAGA CCAGCGTGGA AATTCGCATT TGGGGAGGCA TCAAACAGCT TCTTCATATT 
TTACAAGGAG ACAGAAATTT TGTTTCTGAT CACTCCTCCA TTGGAAGCCT GTCCAGTGCA 
AATGCTGCAG GCCGAATCCA GCAGCTTCAT TTATCAGAAG ACTTGAGCCC TAGGGAAATA 
CAAGAAAATA CTTTCTCACT TCAAGCAGCC TGCTGTGCTG CCCTCACTGA GCTGGTGCTC 
AATGACACCA ATGCCCACCA GGTGGTTCAG GAAAATGGTG TATATACAAT AGCAAAATTA 
ATTTTACCAA ATAAGCAAAA GAATGCAGCA AAAAGTAATC TATTACAGTG TTATGCTTTC 
AGAGCCTTGA GATTTCTCTT CAGTATGGAA AGAAACAGAC CACTCTTTAA AAGACTTTTC 
CCCACAGACT TGTTTGAGAT CTTCATTGAC ATAGGGCATT ATGTACGTGA TATCAGTGCT 
TATGAAGAAT TGGTATCCAA GCTGAATTTA TTAGTGGAGG ATGAACTGAA GCAAATTGCT 
GAAAATATTG AAAGCATTAA TCAGAACAAA GCTCCTTTGA AATATATAGG CAACTATGCA 
ATTTTGGATC ATCTTGGAAG TGGAGCTTTT GGCTGTGTTT ACAAGGTTAG AAAGCATAGT 
GGTCAAAATC TTTTAGCAAT GAAAGAGGTC AATTTACATA ACCCAGCATT TGGAAAGGAT 
AAGAAAGATC GAGACAGCAG CGTAAGGAAT ATTGTTTCTG AATTAACAAT AATTAAAGAG 
CAGCTTTATC ATCCCAACAT TGTACGTTAT TACAAAACAT TTCTGGAAAA TGATAGGTTG 
TACATAGTTA TGGAGCTGAT AGAAGGAGCC CCGCTTGGAG AGCATTTCAG TTCTTTGAAG 
GAAAAACATC ACCATTTTAC TGAAGAAAGA CTATGGAAAA TATTTATACA GCTGTGCTTA 
GCTCTTCGAT ACTTACACAA GGAGAAGAGG ATTGTCCATA GAGATCTGAC ACCAAACAAC 
ATTATGTTGG GGGATAAGGA CAAAGTAACC GTTACTGACT TTGGCCTGGC AAAGCAAAAA 
CAAGAAAACA GTAAACTCAC CTCTGTGGTT GGAACAATCC TGTATTCTTG CCCCGAGGTA 
CTGAAGAGTG AGCCGTATGG GGAGAAGGCT GATGTCTGGG CAGTAGGCTG CATCCTTTAT 
CAGATGGCGA CTTTGAGTCC CCCCTTCTAC AGCACTAACA TGCTGTCCTT GGCTACAAAA 
ATAGTGGAGG CGGTATATGA ACCAGTCCCA GAAGGTATCT ACTCTGAAAA AGTAACAGAC 
ACCATCAGCA GGTGCCTCAC TCCTGATGCG GAAGCTCGTC CAGATATTGT AGAAGTCAGT 
TCGATGATAT CAGATGTCAT GATGAAATAT TTAGACAACT TATCTACATC CCAGTTGTCC 
TTGGAAAAGA AGCTAGAACG GGAACGAAGA CGCACACAAA GGTATTTTAT GGAAGCCAAC 
CGGAACACCG TCACATGTCA CCATGAGCTG GCTGTTCTAT CTCACGAGAC CTTTGAGAAG 
GCAAGTTTGA GTAGCAGCAG CAGTGGAGCA GCCAGCCTGA AAAGTGAACT TTCAGAAAGC 
GCAGACCTGC CCCCTGAAGG CTTCCAGGCC TCCTATGGTA AAGACGAAGA CAGGGCCTGT 
GACGAAATCC TGTCAGATGA TAACTTCAAC CTGGAAAATG CTGAGAAAGA TACATATTCA 
GAGGTAGATG ATGAATTGGA CATTTCGGAT AACTCCAGCA GCTCCAGTTC AAGCCCTCTG 
AAAGAATCTA CATTCAACAT TTTAAAGAGA AGTTTTAGTG CTTCAGGAGG AGAAAGACAA 
TCCCAAACAA GGGACTTCAC TGGAGGAACA GGATCAAGAC CAAGACCAGC TTTGCTGCCT 
CTTGACCTGC TTCTGAAAGT GCCACCCCAC ATGCTCAGGG CCCACATTAA GGAAATAGAG 
GCTGAGTTAG TGACAGGGTG GCAGTCCCAT AGCCTTCCTG CTGTGATTCT TCGAAATCTC 
AAAGATCATG GGCCACAGAT GGGCACATTC TTGTGGCAAG CATCAGCAGG AATTGCTGTG 
TCCCAGAGGA AAGTGCGTCA GATCAGTGAT CCTATTCAGC AGATATTAAT TCAGCTGCAC 
AAAATAATCT ATATCACACA GCTTCCTCCA GCTTTGCACC ACAATTTGAA AAGAAGGGTT 
ATAGAGAGAT TCAAGAAATC CCTCTTCAGC CAGCAGAGTA ACCCTTGTAA TTTGAAATCT 
GAAATTAAAA AGTTATCTCA GGGATCTCCA GAACCGATTG AGCCCAACTT TTTCACAGCA 
GATTACCATT TATTACATCG TTCATCCGGT GGAAACAGCC TGTCCCCAAA TGACCCTACA 
GGTTTACCAA CCAGCATTGA ATTGGAGGAA GGAATAACAT ATGAACAGAT GCAGACTGTG 
ATTGAAGAAG TCCTTGAGGA AAGTGGCTAT TACAATTTTA CATCTAACAG TGTATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006713062.1
CDS	275..3751
Translation	MPDQDKKVKTTEKSTDKQQEITIRDYSDLKRLRCLLNVQSSKQQLPAINFDSAQNSMTKSEPAIRAGGHRARGQWHESTEAVELENFSINYKNERNFSKHPQRKLFQEIFTALVKNRLISREWVNRAPSIHFLRVLICLRLLMRDPCYQEILHSLGGIENLAQYMEIVANEYLGYGEEQHTVDKLVNMTYIFQKLAAVKDQREWVTTSGAHKTLVNLLGARDTNVLLGSLLALASLAESQECREKISELNIVENLLMILHEYDLLSKRLTAELLRLLCAEPQVKEQVKLYEGIPVLLSLLHSDHLKLLWSIVWILVQVCEDPETSVEIRIWGGIKQLLHILQGDRNFVSDHSSIGSLSSANAAGRIQQLHLSEDLSPREIQENTFSLQAACCAALTELVLNDTNAHQVVQENGVYTIAKLILPNKQKNAAKSNLLQCYAFRALRFLFSMERNRPLFKRLFPTDLFEIFIDIGHYVRDISAYEELVSKLNLLVEDELKQIAENIESINQNKAPLKYIGNYAILDHLGSGAFGCVYKVRKHSGQNLLAMKEVNLHNPAFGKDKKDRDSSVRNIVSELTIIKEQLYHPNIVRYYKTFLENDRLYIVMELIEGAPLGEHFSSLKEKHHHFTEERLWKIFIQLCLALRYLHKEKRIVHRDLTPNNIMLGDKDKVTVTDFGLAKQKQENSKLTSVVGTILYSCPEVLKSEPYGEKADVWAVGCILYQMATLSPPFYSTNMLSLATKIVEAVYEPVPEGIYSEKVTDTISRCLTPDAEARPDIVEVSSMISDVMMKYLDNLSTSQLSLEKKLERERRRTQRYFMEANRNTVTCHHELAVLSHETFEKASLSSSSSGAASLKSELSESADLPPEGFQASYGKDEDRACDEILSDDNFNLENAEKDTYSEVDDELDISDNSSSSSSSPLKESTFNILKRSFSASGGERQSQTRDFTGGTGSRPRPALLPLDLLLKVPPHMLRAHIKEIEAELVTGWQSHSLPAVILRNLKDHGPQMGTFLWQASAGIAVSQRKVRQISDPIQQILIQLHKIIYITQLPPALHHNLKRRVIERFKKSLFSQQSNPCNLKSEIKKLSQGSPEPIEPNFFTADYHLLHRSSGGNSLSPNDPTGLPTSIELEEGITYEQMQTVIEEVLEESGYYNFTSNSV

Target ORF information:

RefSeq Version	XM_006712999.3
Organism	Homo sapiens(human)
Definition	Homo sapiens NIMA related kinase 10 (NEK10), transcript variant X10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006712999.3

ORF Insert Sequence:

ATGCCTGATC AAGATAAAAA GGTGAAGACC ACAGAAAAAT CAACTGATAA ACAGCAAGAA 
ATCACCATCA GGGACTATTC AGATCTTAAA AGACTTCGGT GCCTTTTGAA CGTCCAATCA 
AGCAAACAAC AGCTTCCAGC CATTAACTTC GATAGTGCCC AAAATAGCAT GACGAAGTCT 
GAGCCCGCCA TCAGGGCGGG TGGACACAGA GCTCGGGGTC AGTGGCATGA ATCCACAGAA 
GCTGTTGAAC TTGAAAATTT TAGTATAAAC TACAAGAATG AGAGAAATTT CAGCAAACAT 
CCTCAGCGTA AACTATTTCA GGAGATCTTT ACCGCCTTGG TGAAAAATAG ACTCATAAGC 
AGAGAGTGGG TTAATCGAGC CCCATCTATT CATTTTCTGA GAGTGTTAAT CTGTCTGAGG 
CTACTAATGA GGGATCCATG TTATCAGGAA ATACTCCATA GCTTGGGTGG GATTGAAAAC 
CTAGCTCAGT ATATGGAGAT TGTAGCCAAT GAGTACCTCG GCTATGGAGA AGAGCAGCAC 
ACTGTGGACA AGCTGGTCAA CATGACATAT ATTTTTCAAA AACTTGCTGC AGTCAAAGAT 
CAAAGAGAAT GGGTCACCAC AAGTGGAGCC CACAAGACAT TAGTAAATTT ACTTGGTGCC 
CGAGATACTA ATGTTCTATT GGGTTCCCTT CTGGCTCTGG CTAGTTTAGC AGAAAGTCAA 
GAATGTAGGG AGAAGATAAG TGAACTCAAC ATTGTAGAAA ATCTGTTGAT GATTTTACAT 
GAATATGACT TGCTTTCTAA AAGACTAACA GCGGAGTTGC TGCGCCTACT TTGTGCAGAG 
CCCCAGGTGA AAGAGCAGGT GAAGCTCTAT GAGGGGATAC CGGTCCTCCT CAGTCTGCTC 
CACTCTGACC ACTTGAAGCT CCTCTGGAGC ATTGTCTGGA TTCTGGTACA GGTTTGTGAG 
GACCCTGAGA CCAGCGTGGA AATTCGCATT TGGGGAGGCA TCAAACAGCT TCTTCATATT 
TTACAAGGAG ACAGAAATTT TGTTTCTGAT CACTCCTCCA TTGGAAGCCT GTCCAGTGCA 
AATGCTGCAG GCCGAATCCA GCAGCTTCAT TTATCAGAAG ACTTGAGCCC TAGGGAAATA 
CAAGAAAATA CTTTCTCACT TCAAGCAGCC TGCTGTGCTG CCCTCACTGA GCTGGTGCTC 
AATGACACCA ATGCCCACCA GGTGGTTCAG GAAAATGGTG TATATACAAT AGCAAAATTA 
ATTTTACCAA ATAAGCAAAA GAATGCAGCA AAAAGTAATC TATTACAGTG TTATGCTTTC 
AGAGCCTTGA GATTTCTCTT CAGTATGGAA AGAAACAGAC CACTCTTTAA AAGACTTTTC 
CCCACAGACT TGTTTGAGAT CTTCATTGAC ATAGGGCATT ATGTACGTGA TATCAGTGCT 
TATGAAGAAT TGGTATCCAA GCTGAATTTA TTAGTGGAGG ATGAACTGAA GCAAATTGCT 
GAAAATATTG AAAGCATTAA TCAGAACAAA GCTCCTTTGA AATATATAGG CAACTATGCA 
ATTTTGGATC ATCTTGGAAG TGGAGCTTTT GGCTGTGTTT ACAAGGTTAG AAAGCATAGT 
GGTCAAAATC TTTTAGCAAT GAAAGAGGTC AATTTACATA ACCCAGCATT TGGAAAGGAT 
AAGAAAGATC GAGACAGCAG CGTAAGGAAT ATTGTTTCTG AATTAACAAT AATTAAAGAG 
CAGCTTTATC ATCCCAACAT TGTACGTTAT TACAAAACAT TTCTGGAAAA TGATAGGTTG 
TACATAGTTA TGGAGCTGAT AGAAGGAGCC CCGCTTGGAG AGCATTTCAG TTCTTTGAAG 
GAAAAACATC ACCATTTTAC TGAAGAAAGA CTATGGAAAA TATTTATACA GCTGTGCTTA 
GCTCTTCGAT ACTTACACAA GGAGAAGAGG ATTGTCCATA GAGATCTGAC ACCAAACAAC 
ATTATGTTGG GGGATAAGGA CAAAGTAACC GTTACTGACT TTGGCCTGGC AAAGCAAAAA 
CAAGAAAACA GTAAACTCAC CTCTGTGGTT GGAACAATCC TGTATTCTTG CCCCGAGGTA 
CTGAAGAGTG AGCCGTATGG GGAGAAGGCT GATGTCTGGG CAGTAGGCTG CATCCTTTAT 
CAGATGGCGA CTTTGAGTCC CCCCTTCTAC AGCACTAACA TGCTGTCCTT GGCTACAAAA 
ATAGTGGAGG CGGTATATGA ACCAGTCCCA GAAGGTATCT ACTCTGAAAA AGTAACAGAC 
ACCATCAGCA GGTGCCTCAC TCCTGATGCG GAAGCTCGTC CAGATATTGT AGAAGTCAGT 
TCGATGATAT CAGATGTCAT GATGAAATAT TTAGACAACT TATCTACATC CCAGTTGTCC 
TTGGAAAAGA AGCTAGAACG GGAACGAAGA CGCACACAAA GGTATTTTAT GGAAGCCAAC 
CGGAACACCG TCACATGTCA CCATGAGCTG GCTGTTCTAT CTCACGAGAC CTTTGAGAAG 
GCAAGTTTGA GTAGCAGCAG CAGTGGAGCA GCCAGCCTGA AAAGTGAACT TTCAGAAAGC 
GCAGACCTGC CCCCTGAAGG CTTCCAGGCC TCCTATGGTA AAGACGAAGA CAGGGCCTGT 
GACGAAATCC TGTCAGATGA TAACTTCAAC CTGGAAAATG CTGAGAAAGA TACATATTCA 
GAGGTAGATG ATGAATTGGA CATTTCGGAT AACTCCAGCA GCTCCAGTTC AAGCCCTCTG 
AAAGAATCTA CATTCAACAT TTTAAAGAGA AGTTTTAGTG CTTCAGGAGG AGAAAGACAA 
TCCCAAACAA GGGACTTCAC TGGAGGAACA GGATCAAGAC CAAGACCAGC TTTGCTGCCT 
CTTGACCTGC TTCTGAAAGT GCCACCCCAC ATGCTCAGGG CCCACATTAA GGAAATAGAG 
GCTGAGTTAG TGACAGGGTG GCAGTCCCAT AGCCTTCCTG CTGTGATTCT TCGAAATCTC 
AAAGATCATG GGCCACAGAT GGGCACATTC TTGTGGCAAG CATCAGCAGG AATTGCTGTG 
TCCCAGAGGA AAGTGCGTCA GATCAGTGAT CCTATTCAGC AGATATTAAT TCAGCTGCAC 
AAAATAATCT ATATCACACA GCTTCCTCCA GCTTTGCACC ACAATTTGAA AAGAAGGGTT 
ATAGAGAGAT TCAAGAAATC CCTCTTCAGC CAGCAGAGTA ACCCTTGTAA TTTGAAATCT 
GAAATTAAAA AGTTATCTCA GGGATCTCCA GAACCGATTG AGCCCAACTT TTTCACAGCA 
GATTACCATT TATTACATCG TTCATCCGGT GGAAACAGCC TGTCCCCAAA TGACCCTACA 
GGTTTACCAA CCAGCATTGA ATTGGAGGAA GGAATAACAT ATGAACAGAT GCAGACTGTG 
ATTGAAGAAG TCCTTGAGGA AAGTGGCTAT TACAATTTTA CATCTAACAG TGTATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NEK10 ( XM_006713001.3 ) cDNA ORF clone, Homo sapiens(human) -> XP_006713064.1 Homo sapiens NIMA related kinase 10 (NEK10), transcript variant X16, mRNA.

Price & Availability↑

CloneID	OHu36996
Clone ID Related Accession (Same CDS sequence)	XM_006713001.3 , XM_017005771.1 , XM_017005771.2
Accession Version	XM_006713001.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3348bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	serine/threonine-protein kinase Nek10 isoform X8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000003.12) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_006713001.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCTGATC AAGATAAAAA GGTGAAGACC ACAGAAAAAT CAACTGATAA ACAGCAAGAA 
ATCACCATCA GGGACTATTC AGATCTTAAA AGACTTCGGT GCCTTTTGAA CGTCCAATCA 
AGCAAACAAC AGCTTCCAGC CATTAACTTC GATAGTGCCC AAAATAGCAT GACGAAGTCT 
GAGCCCGCCA TCAGGGCGGG TGGACACAGA GCTCGGGGTC AGTGGCATGA ATCCACAGAA 
GCTGTTGAAC TTGAAAATTT TAGTATAAAC TACAAGAATG AGAGAAATTT CAGCAAACAT 
CCTCAGCGTA AACTATTTCA GGAGATCTTT ACCGCCTTGG TGAAAAATAG ACTCATAAGC 
AGAGAGTGGG TTAATCGAGC CCCATCTATT CATTTTCTGA GAGTGTTAAT CTGTCTGAGG 
CTACTAATGA GGGATCCATG TTATCAGGAA ATACTCCATA GCTTGGGTGG GATTGAAAAC 
CTAGCTCAGT ATATGGAGAT TGTAGCCAAT GAGTACCTCG GCTATGGAGA AGAGCAGCAC 
ACTGTGGACA AGCTGGTCAA CATGACATAT ATTTTTCAAA AACTTGCTGC AGTCAAAGAT 
CAAAGAGAAT GGGTCACCAC AAGTGGAGCC CACAAGACAT TAGTAAATTT ACTTGGTGCC 
CGAGATACTA ATGTTCTATT GGGTTCCCTT CTGGCTCTGG CTAGTTTAGC AGAAAGTCAA 
GAATGTAGGG AGAAGATAAG TGAACTCAAC ATTGTAGAAA ATCTGTTGAT GATTTTACAT 
GAATATGACT TGCTTTCTAA AAGACTAACA GCGGAGTTGC TGCGCCTACT TTGTGCAGAG 
CCCCAGGTGA AAGAGCAGGT GAAGCTCTAT GAGGGGATAC CGGTCCTCCT CAGTCTGCTC 
CACTCTGACC ACTTGAAGCT CCTCTGGAGC ATTGTCTGGA TTCTGGTACA GGTTTGTGAG 
GACCCTGAGA CCAGCGTGGA AATTCGCATT TGGGGAGGCA TCAAACAGCT TCTTCATATT 
TTACAAGGAG ACAGAAATTT TGTTTCTGAT CACTCCTCCA TTGGAAGCCT GTCCAGTGCA 
AATGCTGCAG GCCGAATCCA GCAGCTTCAT TTATCAGAAG ACTTGAGCCC TAGGGAAATA 
CAAGAAAATA CTTTCTCACT TCAAGCAGCC TGCTGTGCTG CCCTCACTGA GCTGGTGCTC 
AATGACACCA ATGCCCACCA GGTGGTTCAG GAAAATGGTG TATATACAAT AGCAAAATTA 
ATTTTACCAA ATAAGCAAAA GAATGCAGCA AAAAGTAATC TATTACAGTG TTATGCTTTC 
AGAGCCTTGA GATTTCTCTT CAGTATGGAA AGAAACAGAC CACTCTTTAA AAGACTTTTC 
CCCACAGACT TGTTTGAGAT CTTCATTGAC ATAGGGCATT ATGTACGTGA TATCAGTGCT 
TATGAAGAAT TGGTATCCAA GCTGAATTTA TTAGTGGAGG ATGAACTGAA GCAAATTGCT 
GAAAATATTG AAAGCATTAA TCAGAACAAA GCTCCTTTGA AATATATAGG CAACTATGCA 
ATTTTGGATC ATCTTGGAAG TGGAGCTTTT GGCTGTGTTT ACAAGGTTAG AAAGCATAGT 
GGTCAAAATC TTTTAGCAAT GAAAGAGGTC AATTTACATA ACCCAGCATT TGGAAAGGAT 
AAGAAAGATC GAGACAGCAG CGTAAGGAAT ATTGTTTCTG AATTAACAAT AATTAAAGAG 
CAGCTTTATC ATCCCAACAT TGTACGTTAT TACAAAACAT TTCTGGAAAA TGATAGGTTG 
TACATAGTTA TGGAGCTGAT AGAAGGAGCC CCGCTTGGAG AGCATTTCAG TTCTTTGAAG 
GAAAAACATC ACCATTTTAC TGAAGAAAGA CTATGGAAAA TATTTATACA GCTGTGCTTA 
GCTCTTCGAT ACTTACACAA GGAGAAGAGG ATTGTCCATA GAGATCTGAC ACCAAACAAC 
ATTATGTTGG GGGATAAGGA CAAAGTAACC GTTACTGACT TTGGCCTGGC AAAGCAAAAA 
CAAGAAAACA GTAAACTCAC CTCTGTGGTT GGAACAATCC TGTATTCTTG CCCCGAGGTA 
CTGAAGAGTG AGCCGTATGG GGAGAAGGCT GATGTCTGGG CAGTAGGCTG CATCCTTTAT 
CAGATGGCGA CTTTGAGTCC CCCCTTCTAC AGCACTAACA TGCTGTCCTT GGCTACAAAA 
ATAGTGGAGG CGGTATATGA ACCAGTCCCA GAAGGTATCT ACTCTGAAAA AGTAACAGAC 
ACCATCAGCA GGTGCCTCAC TCCTGATGCG GAAGCTCGTC CAGATATTGT AGAAGTCAGT 
TCGATGATAT CAGATGTCAT GATGAAATAT TTAGACAACT TATCTACATC CCAGTTGTCC 
TTGGAAAAGA AGCTAGAACG GGAACGAAGA CGCACACAAA GGTATTTTAT GGAAGCCAAC 
CGGAACACCG TCACATGTCA CCATGAGCTG GCTGTTCTAT CTCACGAGAC CTTTGAGAAG 
GCAAGTTTGA GTAGCAGCAG CAGTGGAGCA GCCAGCCTGA AAAGTGAACT TTCAGAAAGC 
GCAGACCTGC CCCCTGAAGG CTTCCAGGCC TCCTATGGTA AAGACGAAGA CAGGGCCTGT 
GACGAAATCC TGTCAGATGA TAACTTCAAC CTGGAAAATG CTGAGAAAGA TACATATTCA 
GAGGTAGATG ATGAATTGGA CATTTCGGAT AACTCCAGCA GCTCCAGTTC AAGCCCTCTG 
AAAGAATCTA CATTCAACAT TTTAAAGAGA AGTTTTAGTG CTTCAGGAGG AGAAAGACAA 
TCCCAAACAA GGGACTTCAC TGGAGGAACA GGATCAAGAC CAAGACCAGC ATCAGCAGGA 
ATTGCTGTGT CCCAGAGGAA AGTGCGTCAG ATCAGTGATC CTATTCAGCA GATATTAATT 
CAGCTGCACA AAATAATCTA TATCACACAG CTTCCTCCAG CTTTGCACCA CAATTTGAAA 
AGAAGGGTTA TAGAGAGATT CAAGAAATCC CTCTTCAGCC AGCAGAGTAA CCCTTGTAAT 
TTGAAATCTG AAATTAAAAA GTTATCTCAG GGATCTCCAG AACCGATTGA GCCCAACTTT 
TTCACAGCAG ATTACCATTT ATTACATCGT TCATCCGGTG GAAACAGCCT GTCCCCAAAT 
GACCCTACAG GTTTACCAAC CAGCATTGAA TTGGAGGAAG GAATAACATA TGAACAGATG 
CAGACTGTGA TTGAAGAAGT CCTTGAGGAA AGTGGCTATT ACAATTTTAC ATCTAACAGG 
TATCATTCCT ATCCATGGGG GACCAAGAAT CACCCAACCA AAAGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006713064.1
CDS	275..3622
Translation	MPDQDKKVKTTEKSTDKQQEITIRDYSDLKRLRCLLNVQSSKQQLPAINFDSAQNSMTKSEPAIRAGGHRARGQWHESTEAVELENFSINYKNERNFSKHPQRKLFQEIFTALVKNRLISREWVNRAPSIHFLRVLICLRLLMRDPCYQEILHSLGGIENLAQYMEIVANEYLGYGEEQHTVDKLVNMTYIFQKLAAVKDQREWVTTSGAHKTLVNLLGARDTNVLLGSLLALASLAESQECREKISELNIVENLLMILHEYDLLSKRLTAELLRLLCAEPQVKEQVKLYEGIPVLLSLLHSDHLKLLWSIVWILVQVCEDPETSVEIRIWGGIKQLLHILQGDRNFVSDHSSIGSLSSANAAGRIQQLHLSEDLSPREIQENTFSLQAACCAALTELVLNDTNAHQVVQENGVYTIAKLILPNKQKNAAKSNLLQCYAFRALRFLFSMERNRPLFKRLFPTDLFEIFIDIGHYVRDISAYEELVSKLNLLVEDELKQIAENIESINQNKAPLKYIGNYAILDHLGSGAFGCVYKVRKHSGQNLLAMKEVNLHNPAFGKDKKDRDSSVRNIVSELTIIKEQLYHPNIVRYYKTFLENDRLYIVMELIEGAPLGEHFSSLKEKHHHFTEERLWKIFIQLCLALRYLHKEKRIVHRDLTPNNIMLGDKDKVTVTDFGLAKQKQENSKLTSVVGTILYSCPEVLKSEPYGEKADVWAVGCILYQMATLSPPFYSTNMLSLATKIVEAVYEPVPEGIYSEKVTDTISRCLTPDAEARPDIVEVSSMISDVMMKYLDNLSTSQLSLEKKLERERRRTQRYFMEANRNTVTCHHELAVLSHETFEKASLSSSSSGAASLKSELSESADLPPEGFQASYGKDEDRACDEILSDDNFNLENAEKDTYSEVDDELDISDNSSSSSSSPLKESTFNILKRSFSASGGERQSQTRDFTGGTGSRPRPASAGIAVSQRKVRQISDPIQQILIQLHKIIYITQLPPALHHNLKRRVIERFKKSLFSQQSNPCNLKSEIKKLSQGSPEPIEPNFFTADYHLLHRSSGGNSLSPNDPTGLPTSIELEEGITYEQMQTVIEEVLEESGYYNFTSNRYHSYPWGTKNHPTKR

Target ORF information:

RefSeq Version	XM_006713001.3
Organism	Homo sapiens(human)
Definition	Homo sapiens NIMA related kinase 10 (NEK10), transcript variant X16, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006713001.3

ORF Insert Sequence:

ATGCCTGATC AAGATAAAAA GGTGAAGACC ACAGAAAAAT CAACTGATAA ACAGCAAGAA 
ATCACCATCA GGGACTATTC AGATCTTAAA AGACTTCGGT GCCTTTTGAA CGTCCAATCA 
AGCAAACAAC AGCTTCCAGC CATTAACTTC GATAGTGCCC AAAATAGCAT GACGAAGTCT 
GAGCCCGCCA TCAGGGCGGG TGGACACAGA GCTCGGGGTC AGTGGCATGA ATCCACAGAA 
GCTGTTGAAC TTGAAAATTT TAGTATAAAC TACAAGAATG AGAGAAATTT CAGCAAACAT 
CCTCAGCGTA AACTATTTCA GGAGATCTTT ACCGCCTTGG TGAAAAATAG ACTCATAAGC 
AGAGAGTGGG TTAATCGAGC CCCATCTATT CATTTTCTGA GAGTGTTAAT CTGTCTGAGG 
CTACTAATGA GGGATCCATG TTATCAGGAA ATACTCCATA GCTTGGGTGG GATTGAAAAC 
CTAGCTCAGT ATATGGAGAT TGTAGCCAAT GAGTACCTCG GCTATGGAGA AGAGCAGCAC 
ACTGTGGACA AGCTGGTCAA CATGACATAT ATTTTTCAAA AACTTGCTGC AGTCAAAGAT 
CAAAGAGAAT GGGTCACCAC AAGTGGAGCC CACAAGACAT TAGTAAATTT ACTTGGTGCC 
CGAGATACTA ATGTTCTATT GGGTTCCCTT CTGGCTCTGG CTAGTTTAGC AGAAAGTCAA 
GAATGTAGGG AGAAGATAAG TGAACTCAAC ATTGTAGAAA ATCTGTTGAT GATTTTACAT 
GAATATGACT TGCTTTCTAA AAGACTAACA GCGGAGTTGC TGCGCCTACT TTGTGCAGAG 
CCCCAGGTGA AAGAGCAGGT GAAGCTCTAT GAGGGGATAC CGGTCCTCCT CAGTCTGCTC 
CACTCTGACC ACTTGAAGCT CCTCTGGAGC ATTGTCTGGA TTCTGGTACA GGTTTGTGAG 
GACCCTGAGA CCAGCGTGGA AATTCGCATT TGGGGAGGCA TCAAACAGCT TCTTCATATT 
TTACAAGGAG ACAGAAATTT TGTTTCTGAT CACTCCTCCA TTGGAAGCCT GTCCAGTGCA 
AATGCTGCAG GCCGAATCCA GCAGCTTCAT TTATCAGAAG ACTTGAGCCC TAGGGAAATA 
CAAGAAAATA CTTTCTCACT TCAAGCAGCC TGCTGTGCTG CCCTCACTGA GCTGGTGCTC 
AATGACACCA ATGCCCACCA GGTGGTTCAG GAAAATGGTG TATATACAAT AGCAAAATTA 
ATTTTACCAA ATAAGCAAAA GAATGCAGCA AAAAGTAATC TATTACAGTG TTATGCTTTC 
AGAGCCTTGA GATTTCTCTT CAGTATGGAA AGAAACAGAC CACTCTTTAA AAGACTTTTC 
CCCACAGACT TGTTTGAGAT CTTCATTGAC ATAGGGCATT ATGTACGTGA TATCAGTGCT 
TATGAAGAAT TGGTATCCAA GCTGAATTTA TTAGTGGAGG ATGAACTGAA GCAAATTGCT 
GAAAATATTG AAAGCATTAA TCAGAACAAA GCTCCTTTGA AATATATAGG CAACTATGCA 
ATTTTGGATC ATCTTGGAAG TGGAGCTTTT GGCTGTGTTT ACAAGGTTAG AAAGCATAGT 
GGTCAAAATC TTTTAGCAAT GAAAGAGGTC AATTTACATA ACCCAGCATT TGGAAAGGAT 
AAGAAAGATC GAGACAGCAG CGTAAGGAAT ATTGTTTCTG AATTAACAAT AATTAAAGAG 
CAGCTTTATC ATCCCAACAT TGTACGTTAT TACAAAACAT TTCTGGAAAA TGATAGGTTG 
TACATAGTTA TGGAGCTGAT AGAAGGAGCC CCGCTTGGAG AGCATTTCAG TTCTTTGAAG 
GAAAAACATC ACCATTTTAC TGAAGAAAGA CTATGGAAAA TATTTATACA GCTGTGCTTA 
GCTCTTCGAT ACTTACACAA GGAGAAGAGG ATTGTCCATA GAGATCTGAC ACCAAACAAC 
ATTATGTTGG GGGATAAGGA CAAAGTAACC GTTACTGACT TTGGCCTGGC AAAGCAAAAA 
CAAGAAAACA GTAAACTCAC CTCTGTGGTT GGAACAATCC TGTATTCTTG CCCCGAGGTA 
CTGAAGAGTG AGCCGTATGG GGAGAAGGCT GATGTCTGGG CAGTAGGCTG CATCCTTTAT 
CAGATGGCGA CTTTGAGTCC CCCCTTCTAC AGCACTAACA TGCTGTCCTT GGCTACAAAA 
ATAGTGGAGG CGGTATATGA ACCAGTCCCA GAAGGTATCT ACTCTGAAAA AGTAACAGAC 
ACCATCAGCA GGTGCCTCAC TCCTGATGCG GAAGCTCGTC CAGATATTGT AGAAGTCAGT 
TCGATGATAT CAGATGTCAT GATGAAATAT TTAGACAACT TATCTACATC CCAGTTGTCC 
TTGGAAAAGA AGCTAGAACG GGAACGAAGA CGCACACAAA GGTATTTTAT GGAAGCCAAC 
CGGAACACCG TCACATGTCA CCATGAGCTG GCTGTTCTAT CTCACGAGAC CTTTGAGAAG 
GCAAGTTTGA GTAGCAGCAG CAGTGGAGCA GCCAGCCTGA AAAGTGAACT TTCAGAAAGC 
GCAGACCTGC CCCCTGAAGG CTTCCAGGCC TCCTATGGTA AAGACGAAGA CAGGGCCTGT 
GACGAAATCC TGTCAGATGA TAACTTCAAC CTGGAAAATG CTGAGAAAGA TACATATTCA 
GAGGTAGATG ATGAATTGGA CATTTCGGAT AACTCCAGCA GCTCCAGTTC AAGCCCTCTG 
AAAGAATCTA CATTCAACAT TTTAAAGAGA AGTTTTAGTG CTTCAGGAGG AGAAAGACAA 
TCCCAAACAA GGGACTTCAC TGGAGGAACA GGATCAAGAC CAAGACCAGC ATCAGCAGGA 
ATTGCTGTGT CCCAGAGGAA AGTGCGTCAG ATCAGTGATC CTATTCAGCA GATATTAATT 
CAGCTGCACA AAATAATCTA TATCACACAG CTTCCTCCAG CTTTGCACCA CAATTTGAAA 
AGAAGGGTTA TAGAGAGATT CAAGAAATCC CTCTTCAGCC AGCAGAGTAA CCCTTGTAAT 
TTGAAATCTG AAATTAAAAA GTTATCTCAG GGATCTCCAG AACCGATTGA GCCCAACTTT 
TTCACAGCAG ATTACCATTT ATTACATCGT TCATCCGGTG GAAACAGCCT GTCCCCAAAT 
GACCCTACAG GTTTACCAAC CAGCATTGAA TTGGAGGAAG GAATAACATA TGAACAGATG 
CAGACTGTGA TTGAAGAAGT CCTTGAGGAA AGTGGCTATT ACAATTTTAC ATCTAACAGG 
TATCATTCCT ATCCATGGGG GACCAAGAAT CACCCAACCA AAAGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NEK10 ( XM_017005771.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016861260.1 Homo sapiens NIMA related kinase 10 (NEK10), transcript variant X16, mRNA.

Price & Availability↑

CloneID	OHu36996
Clone ID Related Accession (Same CDS sequence)	XM_006713001.3 , XM_017005771.1 , XM_017005771.2
Accession Version	XM_017005771.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3348bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	serine/threonine-protein kinase Nek10 isoform X8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000003.12) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCTGATC AAGATAAAAA GGTGAAGACC ACAGAAAAAT CAACTGATAA ACAGCAAGAA 
ATCACCATCA GGGACTATTC AGATCTTAAA AGACTTCGGT GCCTTTTGAA CGTCCAATCA 
AGCAAACAAC AGCTTCCAGC CATTAACTTC GATAGTGCCC AAAATAGCAT GACGAAGTCT 
GAGCCCGCCA TCAGGGCGGG TGGACACAGA GCTCGGGGTC AGTGGCATGA ATCCACAGAA 
GCTGTTGAAC TTGAAAATTT TAGTATAAAC TACAAGAATG AGAGAAATTT CAGCAAACAT 
CCTCAGCGTA AACTATTTCA GGAGATCTTT ACCGCCTTGG TGAAAAATAG ACTCATAAGC 
AGAGAGTGGG TTAATCGAGC CCCATCTATT CATTTTCTGA GAGTGTTAAT CTGTCTGAGG 
CTACTAATGA GGGATCCATG TTATCAGGAA ATACTCCATA GCTTGGGTGG GATTGAAAAC 
CTAGCTCAGT ATATGGAGAT TGTAGCCAAT GAGTACCTCG GCTATGGAGA AGAGCAGCAC 
ACTGTGGACA AGCTGGTCAA CATGACATAT ATTTTTCAAA AACTTGCTGC AGTCAAAGAT 
CAAAGAGAAT GGGTCACCAC AAGTGGAGCC CACAAGACAT TAGTAAATTT ACTTGGTGCC 
CGAGATACTA ATGTTCTATT GGGTTCCCTT CTGGCTCTGG CTAGTTTAGC AGAAAGTCAA 
GAATGTAGGG AGAAGATAAG TGAACTCAAC ATTGTAGAAA ATCTGTTGAT GATTTTACAT 
GAATATGACT TGCTTTCTAA AAGACTAACA GCGGAGTTGC TGCGCCTACT TTGTGCAGAG 
CCCCAGGTGA AAGAGCAGGT GAAGCTCTAT GAGGGGATAC CGGTCCTCCT CAGTCTGCTC 
CACTCTGACC ACTTGAAGCT CCTCTGGAGC ATTGTCTGGA TTCTGGTACA GGTTTGTGAG 
GACCCTGAGA CCAGCGTGGA AATTCGCATT TGGGGAGGCA TCAAACAGCT TCTTCATATT 
TTACAAGGAG ACAGAAATTT TGTTTCTGAT CACTCCTCCA TTGGAAGCCT GTCCAGTGCA 
AATGCTGCAG GCCGAATCCA GCAGCTTCAT TTATCAGAAG ACTTGAGCCC TAGGGAAATA 
CAAGAAAATA CTTTCTCACT TCAAGCAGCC TGCTGTGCTG CCCTCACTGA GCTGGTGCTC 
AATGACACCA ATGCCCACCA GGTGGTTCAG GAAAATGGTG TATATACAAT AGCAAAATTA 
ATTTTACCAA ATAAGCAAAA GAATGCAGCA AAAAGTAATC TATTACAGTG TTATGCTTTC 
AGAGCCTTGA GATTTCTCTT CAGTATGGAA AGAAACAGAC CACTCTTTAA AAGACTTTTC 
CCCACAGACT TGTTTGAGAT CTTCATTGAC ATAGGGCATT ATGTACGTGA TATCAGTGCT 
TATGAAGAAT TGGTATCCAA GCTGAATTTA TTAGTGGAGG ATGAACTGAA GCAAATTGCT 
GAAAATATTG AAAGCATTAA TCAGAACAAA GCTCCTTTGA AATATATAGG CAACTATGCA 
ATTTTGGATC ATCTTGGAAG TGGAGCTTTT GGCTGTGTTT ACAAGGTTAG AAAGCATAGT 
GGTCAAAATC TTTTAGCAAT GAAAGAGGTC AATTTACATA ACCCAGCATT TGGAAAGGAT 
AAGAAAGATC GAGACAGCAG CGTAAGGAAT ATTGTTTCTG AATTAACAAT AATTAAAGAG 
CAGCTTTATC ATCCCAACAT TGTACGTTAT TACAAAACAT TTCTGGAAAA TGATAGGTTG 
TACATAGTTA TGGAGCTGAT AGAAGGAGCC CCGCTTGGAG AGCATTTCAG TTCTTTGAAG 
GAAAAACATC ACCATTTTAC TGAAGAAAGA CTATGGAAAA TATTTATACA GCTGTGCTTA 
GCTCTTCGAT ACTTACACAA GGAGAAGAGG ATTGTCCATA GAGATCTGAC ACCAAACAAC 
ATTATGTTGG GGGATAAGGA CAAAGTAACC GTTACTGACT TTGGCCTGGC AAAGCAAAAA 
CAAGAAAACA GTAAACTCAC CTCTGTGGTT GGAACAATCC TGTATTCTTG CCCCGAGGTA 
CTGAAGAGTG AGCCGTATGG GGAGAAGGCT GATGTCTGGG CAGTAGGCTG CATCCTTTAT 
CAGATGGCGA CTTTGAGTCC CCCCTTCTAC AGCACTAACA TGCTGTCCTT GGCTACAAAA 
ATAGTGGAGG CGGTATATGA ACCAGTCCCA GAAGGTATCT ACTCTGAAAA AGTAACAGAC 
ACCATCAGCA GGTGCCTCAC TCCTGATGCG GAAGCTCGTC CAGATATTGT AGAAGTCAGT 
TCGATGATAT CAGATGTCAT GATGAAATAT TTAGACAACT TATCTACATC CCAGTTGTCC 
TTGGAAAAGA AGCTAGAACG GGAACGAAGA CGCACACAAA GGTATTTTAT GGAAGCCAAC 
CGGAACACCG TCACATGTCA CCATGAGCTG GCTGTTCTAT CTCACGAGAC CTTTGAGAAG 
GCAAGTTTGA GTAGCAGCAG CAGTGGAGCA GCCAGCCTGA AAAGTGAACT TTCAGAAAGC 
GCAGACCTGC CCCCTGAAGG CTTCCAGGCC TCCTATGGTA AAGACGAAGA CAGGGCCTGT 
GACGAAATCC TGTCAGATGA TAACTTCAAC CTGGAAAATG CTGAGAAAGA TACATATTCA 
GAGGTAGATG ATGAATTGGA CATTTCGGAT AACTCCAGCA GCTCCAGTTC AAGCCCTCTG 
AAAGAATCTA CATTCAACAT TTTAAAGAGA AGTTTTAGTG CTTCAGGAGG AGAAAGACAA 
TCCCAAACAA GGGACTTCAC TGGAGGAACA GGATCAAGAC CAAGACCAGC ATCAGCAGGA 
ATTGCTGTGT CCCAGAGGAA AGTGCGTCAG ATCAGTGATC CTATTCAGCA GATATTAATT 
CAGCTGCACA AAATAATCTA TATCACACAG CTTCCTCCAG CTTTGCACCA CAATTTGAAA 
AGAAGGGTTA TAGAGAGATT CAAGAAATCC CTCTTCAGCC AGCAGAGTAA CCCTTGTAAT 
TTGAAATCTG AAATTAAAAA GTTATCTCAG GGATCTCCAG AACCGATTGA GCCCAACTTT 
TTCACAGCAG ATTACCATTT ATTACATCGT TCATCCGGTG GAAACAGCCT GTCCCCAAAT 
GACCCTACAG GTTTACCAAC CAGCATTGAA TTGGAGGAAG GAATAACATA TGAACAGATG 
CAGACTGTGA TTGAAGAAGT CCTTGAGGAA AGTGGCTATT ACAATTTTAC ATCTAACAGG 
TATCATTCCT ATCCATGGGG GACCAAGAAT CACCCAACCA AAAGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016861260.1
CDS	227..3574
Translation	MPDQDKKVKTTEKSTDKQQEITIRDYSDLKRLRCLLNVQSSKQQLPAINFDSAQNSMTKSEPAIRAGGHRARGQWHESTEAVELENFSINYKNERNFSKHPQRKLFQEIFTALVKNRLISREWVNRAPSIHFLRVLICLRLLMRDPCYQEILHSLGGIENLAQYMEIVANEYLGYGEEQHTVDKLVNMTYIFQKLAAVKDQREWVTTSGAHKTLVNLLGARDTNVLLGSLLALASLAESQECREKISELNIVENLLMILHEYDLLSKRLTAELLRLLCAEPQVKEQVKLYEGIPVLLSLLHSDHLKLLWSIVWILVQVCEDPETSVEIRIWGGIKQLLHILQGDRNFVSDHSSIGSLSSANAAGRIQQLHLSEDLSPREIQENTFSLQAACCAALTELVLNDTNAHQVVQENGVYTIAKLILPNKQKNAAKSNLLQCYAFRALRFLFSMERNRPLFKRLFPTDLFEIFIDIGHYVRDISAYEELVSKLNLLVEDELKQIAENIESINQNKAPLKYIGNYAILDHLGSGAFGCVYKVRKHSGQNLLAMKEVNLHNPAFGKDKKDRDSSVRNIVSELTIIKEQLYHPNIVRYYKTFLENDRLYIVMELIEGAPLGEHFSSLKEKHHHFTEERLWKIFIQLCLALRYLHKEKRIVHRDLTPNNIMLGDKDKVTVTDFGLAKQKQENSKLTSVVGTILYSCPEVLKSEPYGEKADVWAVGCILYQMATLSPPFYSTNMLSLATKIVEAVYEPVPEGIYSEKVTDTISRCLTPDAEARPDIVEVSSMISDVMMKYLDNLSTSQLSLEKKLERERRRTQRYFMEANRNTVTCHHELAVLSHETFEKASLSSSSSGAASLKSELSESADLPPEGFQASYGKDEDRACDEILSDDNFNLENAEKDTYSEVDDELDISDNSSSSSSSPLKESTFNILKRSFSASGGERQSQTRDFTGGTGSRPRPASAGIAVSQRKVRQISDPIQQILIQLHKIIYITQLPPALHHNLKRRVIERFKKSLFSQQSNPCNLKSEIKKLSQGSPEPIEPNFFTADYHLLHRSSGGNSLSPNDPTGLPTSIELEEGITYEQMQTVIEEVLEESGYYNFTSNRYHSYPWGTKNHPTKR

Target ORF information:

RefSeq Version	XM_017005771.1
Organism	Homo sapiens(human)
Definition	Homo sapiens NIMA related kinase 10 (NEK10), transcript variant X16, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017005771.1

ORF Insert Sequence:

ATGCCTGATC AAGATAAAAA GGTGAAGACC ACAGAAAAAT CAACTGATAA ACAGCAAGAA 
ATCACCATCA GGGACTATTC AGATCTTAAA AGACTTCGGT GCCTTTTGAA CGTCCAATCA 
AGCAAACAAC AGCTTCCAGC CATTAACTTC GATAGTGCCC AAAATAGCAT GACGAAGTCT 
GAGCCCGCCA TCAGGGCGGG TGGACACAGA GCTCGGGGTC AGTGGCATGA ATCCACAGAA 
GCTGTTGAAC TTGAAAATTT TAGTATAAAC TACAAGAATG AGAGAAATTT CAGCAAACAT 
CCTCAGCGTA AACTATTTCA GGAGATCTTT ACCGCCTTGG TGAAAAATAG ACTCATAAGC 
AGAGAGTGGG TTAATCGAGC CCCATCTATT CATTTTCTGA GAGTGTTAAT CTGTCTGAGG 
CTACTAATGA GGGATCCATG TTATCAGGAA ATACTCCATA GCTTGGGTGG GATTGAAAAC 
CTAGCTCAGT ATATGGAGAT TGTAGCCAAT GAGTACCTCG GCTATGGAGA AGAGCAGCAC 
ACTGTGGACA AGCTGGTCAA CATGACATAT ATTTTTCAAA AACTTGCTGC AGTCAAAGAT 
CAAAGAGAAT GGGTCACCAC AAGTGGAGCC CACAAGACAT TAGTAAATTT ACTTGGTGCC 
CGAGATACTA ATGTTCTATT GGGTTCCCTT CTGGCTCTGG CTAGTTTAGC AGAAAGTCAA 
GAATGTAGGG AGAAGATAAG TGAACTCAAC ATTGTAGAAA ATCTGTTGAT GATTTTACAT 
GAATATGACT TGCTTTCTAA AAGACTAACA GCGGAGTTGC TGCGCCTACT TTGTGCAGAG 
CCCCAGGTGA AAGAGCAGGT GAAGCTCTAT GAGGGGATAC CGGTCCTCCT CAGTCTGCTC 
CACTCTGACC ACTTGAAGCT CCTCTGGAGC ATTGTCTGGA TTCTGGTACA GGTTTGTGAG 
GACCCTGAGA CCAGCGTGGA AATTCGCATT TGGGGAGGCA TCAAACAGCT TCTTCATATT 
TTACAAGGAG ACAGAAATTT TGTTTCTGAT CACTCCTCCA TTGGAAGCCT GTCCAGTGCA 
AATGCTGCAG GCCGAATCCA GCAGCTTCAT TTATCAGAAG ACTTGAGCCC TAGGGAAATA 
CAAGAAAATA CTTTCTCACT TCAAGCAGCC TGCTGTGCTG CCCTCACTGA GCTGGTGCTC 
AATGACACCA ATGCCCACCA GGTGGTTCAG GAAAATGGTG TATATACAAT AGCAAAATTA 
ATTTTACCAA ATAAGCAAAA GAATGCAGCA AAAAGTAATC TATTACAGTG TTATGCTTTC 
AGAGCCTTGA GATTTCTCTT CAGTATGGAA AGAAACAGAC CACTCTTTAA AAGACTTTTC 
CCCACAGACT TGTTTGAGAT CTTCATTGAC ATAGGGCATT ATGTACGTGA TATCAGTGCT 
TATGAAGAAT TGGTATCCAA GCTGAATTTA TTAGTGGAGG ATGAACTGAA GCAAATTGCT 
GAAAATATTG AAAGCATTAA TCAGAACAAA GCTCCTTTGA AATATATAGG CAACTATGCA 
ATTTTGGATC ATCTTGGAAG TGGAGCTTTT GGCTGTGTTT ACAAGGTTAG AAAGCATAGT 
GGTCAAAATC TTTTAGCAAT GAAAGAGGTC AATTTACATA ACCCAGCATT TGGAAAGGAT 
AAGAAAGATC GAGACAGCAG CGTAAGGAAT ATTGTTTCTG AATTAACAAT AATTAAAGAG 
CAGCTTTATC ATCCCAACAT TGTACGTTAT TACAAAACAT TTCTGGAAAA TGATAGGTTG 
TACATAGTTA TGGAGCTGAT AGAAGGAGCC CCGCTTGGAG AGCATTTCAG TTCTTTGAAG 
GAAAAACATC ACCATTTTAC TGAAGAAAGA CTATGGAAAA TATTTATACA GCTGTGCTTA 
GCTCTTCGAT ACTTACACAA GGAGAAGAGG ATTGTCCATA GAGATCTGAC ACCAAACAAC 
ATTATGTTGG GGGATAAGGA CAAAGTAACC GTTACTGACT TTGGCCTGGC AAAGCAAAAA 
CAAGAAAACA GTAAACTCAC CTCTGTGGTT GGAACAATCC TGTATTCTTG CCCCGAGGTA 
CTGAAGAGTG AGCCGTATGG GGAGAAGGCT GATGTCTGGG CAGTAGGCTG CATCCTTTAT 
CAGATGGCGA CTTTGAGTCC CCCCTTCTAC AGCACTAACA TGCTGTCCTT GGCTACAAAA 
ATAGTGGAGG CGGTATATGA ACCAGTCCCA GAAGGTATCT ACTCTGAAAA AGTAACAGAC 
ACCATCAGCA GGTGCCTCAC TCCTGATGCG GAAGCTCGTC CAGATATTGT AGAAGTCAGT 
TCGATGATAT CAGATGTCAT GATGAAATAT TTAGACAACT TATCTACATC CCAGTTGTCC 
TTGGAAAAGA AGCTAGAACG GGAACGAAGA CGCACACAAA GGTATTTTAT GGAAGCCAAC 
CGGAACACCG TCACATGTCA CCATGAGCTG GCTGTTCTAT CTCACGAGAC CTTTGAGAAG 
GCAAGTTTGA GTAGCAGCAG CAGTGGAGCA GCCAGCCTGA AAAGTGAACT TTCAGAAAGC 
GCAGACCTGC CCCCTGAAGG CTTCCAGGCC TCCTATGGTA AAGACGAAGA CAGGGCCTGT 
GACGAAATCC TGTCAGATGA TAACTTCAAC CTGGAAAATG CTGAGAAAGA TACATATTCA 
GAGGTAGATG ATGAATTGGA CATTTCGGAT AACTCCAGCA GCTCCAGTTC AAGCCCTCTG 
AAAGAATCTA CATTCAACAT TTTAAAGAGA AGTTTTAGTG CTTCAGGAGG AGAAAGACAA 
TCCCAAACAA GGGACTTCAC TGGAGGAACA GGATCAAGAC CAAGACCAGC ATCAGCAGGA 
ATTGCTGTGT CCCAGAGGAA AGTGCGTCAG ATCAGTGATC CTATTCAGCA GATATTAATT 
CAGCTGCACA AAATAATCTA TATCACACAG CTTCCTCCAG CTTTGCACCA CAATTTGAAA 
AGAAGGGTTA TAGAGAGATT CAAGAAATCC CTCTTCAGCC AGCAGAGTAA CCCTTGTAAT 
TTGAAATCTG AAATTAAAAA GTTATCTCAG GGATCTCCAG AACCGATTGA GCCCAACTTT 
TTCACAGCAG ATTACCATTT ATTACATCGT TCATCCGGTG GAAACAGCCT GTCCCCAAAT 
GACCCTACAG GTTTACCAAC CAGCATTGAA TTGGAGGAAG GAATAACATA TGAACAGATG 
CAGACTGTGA TTGAAGAAGT CCTTGAGGAA AGTGGCTATT ACAATTTTAC ATCTAACAGG 
TATCATTCCT ATCCATGGGG GACCAAGAAT CACCCAACCA AAAGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NEK10 ( XM_017005771.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_016861260.1 Homo sapiens NIMA related kinase 10 (NEK10), transcript variant X17, mRNA.

Price & Availability↑

CloneID	OHu36996
Clone ID Related Accession (Same CDS sequence)	XM_006713001.3 , XM_017005771.1 , XM_017005771.2
Accession Version	XM_017005771.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3348bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	serine/threonine-protein kinase Nek10 isoform X8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000003.12) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_017005771.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCTGATC AAGATAAAAA GGTGAAGACC ACAGAAAAAT CAACTGATAA ACAGCAAGAA 
ATCACCATCA GGGACTATTC AGATCTTAAA AGACTTCGGT GCCTTTTGAA CGTCCAATCA 
AGCAAACAAC AGCTTCCAGC CATTAACTTC GATAGTGCCC AAAATAGCAT GACGAAGTCT 
GAGCCCGCCA TCAGGGCGGG TGGACACAGA GCTCGGGGTC AGTGGCATGA ATCCACAGAA 
GCTGTTGAAC TTGAAAATTT TAGTATAAAC TACAAGAATG AGAGAAATTT CAGCAAACAT 
CCTCAGCGTA AACTATTTCA GGAGATCTTT ACCGCCTTGG TGAAAAATAG ACTCATAAGC 
AGAGAGTGGG TTAATCGAGC CCCATCTATT CATTTTCTGA GAGTGTTAAT CTGTCTGAGG 
CTACTAATGA GGGATCCATG TTATCAGGAA ATACTCCATA GCTTGGGTGG GATTGAAAAC 
CTAGCTCAGT ATATGGAGAT TGTAGCCAAT GAGTACCTCG GCTATGGAGA AGAGCAGCAC 
ACTGTGGACA AGCTGGTCAA CATGACATAT ATTTTTCAAA AACTTGCTGC AGTCAAAGAT 
CAAAGAGAAT GGGTCACCAC AAGTGGAGCC CACAAGACAT TAGTAAATTT ACTTGGTGCC 
CGAGATACTA ATGTTCTATT GGGTTCCCTT CTGGCTCTGG CTAGTTTAGC AGAAAGTCAA 
GAATGTAGGG AGAAGATAAG TGAACTCAAC ATTGTAGAAA ATCTGTTGAT GATTTTACAT 
GAATATGACT TGCTTTCTAA AAGACTAACA GCGGAGTTGC TGCGCCTACT TTGTGCAGAG 
CCCCAGGTGA AAGAGCAGGT GAAGCTCTAT GAGGGGATAC CGGTCCTCCT CAGTCTGCTC 
CACTCTGACC ACTTGAAGCT CCTCTGGAGC ATTGTCTGGA TTCTGGTACA GGTTTGTGAG 
GACCCTGAGA CCAGCGTGGA AATTCGCATT TGGGGAGGCA TCAAACAGCT TCTTCATATT 
TTACAAGGAG ACAGAAATTT TGTTTCTGAT CACTCCTCCA TTGGAAGCCT GTCCAGTGCA 
AATGCTGCAG GCCGAATCCA GCAGCTTCAT TTATCAGAAG ACTTGAGCCC TAGGGAAATA 
CAAGAAAATA CTTTCTCACT TCAAGCAGCC TGCTGTGCTG CCCTCACTGA GCTGGTGCTC 
AATGACACCA ATGCCCACCA GGTGGTTCAG GAAAATGGTG TATATACAAT AGCAAAATTA 
ATTTTACCAA ATAAGCAAAA GAATGCAGCA AAAAGTAATC TATTACAGTG TTATGCTTTC 
AGAGCCTTGA GATTTCTCTT CAGTATGGAA AGAAACAGAC CACTCTTTAA AAGACTTTTC 
CCCACAGACT TGTTTGAGAT CTTCATTGAC ATAGGGCATT ATGTACGTGA TATCAGTGCT 
TATGAAGAAT TGGTATCCAA GCTGAATTTA TTAGTGGAGG ATGAACTGAA GCAAATTGCT 
GAAAATATTG AAAGCATTAA TCAGAACAAA GCTCCTTTGA AATATATAGG CAACTATGCA 
ATTTTGGATC ATCTTGGAAG TGGAGCTTTT GGCTGTGTTT ACAAGGTTAG AAAGCATAGT 
GGTCAAAATC TTTTAGCAAT GAAAGAGGTC AATTTACATA ACCCAGCATT TGGAAAGGAT 
AAGAAAGATC GAGACAGCAG CGTAAGGAAT ATTGTTTCTG AATTAACAAT AATTAAAGAG 
CAGCTTTATC ATCCCAACAT TGTACGTTAT TACAAAACAT TTCTGGAAAA TGATAGGTTG 
TACATAGTTA TGGAGCTGAT AGAAGGAGCC CCGCTTGGAG AGCATTTCAG TTCTTTGAAG 
GAAAAACATC ACCATTTTAC TGAAGAAAGA CTATGGAAAA TATTTATACA GCTGTGCTTA 
GCTCTTCGAT ACTTACACAA GGAGAAGAGG ATTGTCCATA GAGATCTGAC ACCAAACAAC 
ATTATGTTGG GGGATAAGGA CAAAGTAACC GTTACTGACT TTGGCCTGGC AAAGCAAAAA 
CAAGAAAACA GTAAACTCAC CTCTGTGGTT GGAACAATCC TGTATTCTTG CCCCGAGGTA 
CTGAAGAGTG AGCCGTATGG GGAGAAGGCT GATGTCTGGG CAGTAGGCTG CATCCTTTAT 
CAGATGGCGA CTTTGAGTCC CCCCTTCTAC AGCACTAACA TGCTGTCCTT GGCTACAAAA 
ATAGTGGAGG CGGTATATGA ACCAGTCCCA GAAGGTATCT ACTCTGAAAA AGTAACAGAC 
ACCATCAGCA GGTGCCTCAC TCCTGATGCG GAAGCTCGTC CAGATATTGT AGAAGTCAGT 
TCGATGATAT CAGATGTCAT GATGAAATAT TTAGACAACT TATCTACATC CCAGTTGTCC 
TTGGAAAAGA AGCTAGAACG GGAACGAAGA CGCACACAAA GGTATTTTAT GGAAGCCAAC 
CGGAACACCG TCACATGTCA CCATGAGCTG GCTGTTCTAT CTCACGAGAC CTTTGAGAAG 
GCAAGTTTGA GTAGCAGCAG CAGTGGAGCA GCCAGCCTGA AAAGTGAACT TTCAGAAAGC 
GCAGACCTGC CCCCTGAAGG CTTCCAGGCC TCCTATGGTA AAGACGAAGA CAGGGCCTGT 
GACGAAATCC TGTCAGATGA TAACTTCAAC CTGGAAAATG CTGAGAAAGA TACATATTCA 
GAGGTAGATG ATGAATTGGA CATTTCGGAT AACTCCAGCA GCTCCAGTTC AAGCCCTCTG 
AAAGAATCTA CATTCAACAT TTTAAAGAGA AGTTTTAGTG CTTCAGGAGG AGAAAGACAA 
TCCCAAACAA GGGACTTCAC TGGAGGAACA GGATCAAGAC CAAGACCAGC ATCAGCAGGA 
ATTGCTGTGT CCCAGAGGAA AGTGCGTCAG ATCAGTGATC CTATTCAGCA GATATTAATT 
CAGCTGCACA AAATAATCTA TATCACACAG CTTCCTCCAG CTTTGCACCA CAATTTGAAA 
AGAAGGGTTA TAGAGAGATT CAAGAAATCC CTCTTCAGCC AGCAGAGTAA CCCTTGTAAT 
TTGAAATCTG AAATTAAAAA GTTATCTCAG GGATCTCCAG AACCGATTGA GCCCAACTTT 
TTCACAGCAG ATTACCATTT ATTACATCGT TCATCCGGTG GAAACAGCCT GTCCCCAAAT 
GACCCTACAG GTTTACCAAC CAGCATTGAA TTGGAGGAAG GAATAACATA TGAACAGATG 
CAGACTGTGA TTGAAGAAGT CCTTGAGGAA AGTGGCTATT ACAATTTTAC ATCTAACAGG 
TATCATTCCT ATCCATGGGG GACCAAGAAT CACCCAACCA AAAGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016861260.1
CDS	274..3621
Translation	MPDQDKKVKTTEKSTDKQQEITIRDYSDLKRLRCLLNVQSSKQQLPAINFDSAQNSMTKSEPAIRAGGHRARGQWHESTEAVELENFSINYKNERNFSKHPQRKLFQEIFTALVKNRLISREWVNRAPSIHFLRVLICLRLLMRDPCYQEILHSLGGIENLAQYMEIVANEYLGYGEEQHTVDKLVNMTYIFQKLAAVKDQREWVTTSGAHKTLVNLLGARDTNVLLGSLLALASLAESQECREKISELNIVENLLMILHEYDLLSKRLTAELLRLLCAEPQVKEQVKLYEGIPVLLSLLHSDHLKLLWSIVWILVQVCEDPETSVEIRIWGGIKQLLHILQGDRNFVSDHSSIGSLSSANAAGRIQQLHLSEDLSPREIQENTFSLQAACCAALTELVLNDTNAHQVVQENGVYTIAKLILPNKQKNAAKSNLLQCYAFRALRFLFSMERNRPLFKRLFPTDLFEIFIDIGHYVRDISAYEELVSKLNLLVEDELKQIAENIESINQNKAPLKYIGNYAILDHLGSGAFGCVYKVRKHSGQNLLAMKEVNLHNPAFGKDKKDRDSSVRNIVSELTIIKEQLYHPNIVRYYKTFLENDRLYIVMELIEGAPLGEHFSSLKEKHHHFTEERLWKIFIQLCLALRYLHKEKRIVHRDLTPNNIMLGDKDKVTVTDFGLAKQKQENSKLTSVVGTILYSCPEVLKSEPYGEKADVWAVGCILYQMATLSPPFYSTNMLSLATKIVEAVYEPVPEGIYSEKVTDTISRCLTPDAEARPDIVEVSSMISDVMMKYLDNLSTSQLSLEKKLERERRRTQRYFMEANRNTVTCHHELAVLSHETFEKASLSSSSSGAASLKSELSESADLPPEGFQASYGKDEDRACDEILSDDNFNLENAEKDTYSEVDDELDISDNSSSSSSSPLKESTFNILKRSFSASGGERQSQTRDFTGGTGSRPRPASAGIAVSQRKVRQISDPIQQILIQLHKIIYITQLPPALHHNLKRRVIERFKKSLFSQQSNPCNLKSEIKKLSQGSPEPIEPNFFTADYHLLHRSSGGNSLSPNDPTGLPTSIELEEGITYEQMQTVIEEVLEESGYYNFTSNRYHSYPWGTKNHPTKR

Target ORF information:

RefSeq Version	XM_017005771.2
Organism	Homo sapiens(human)
Definition	Homo sapiens NIMA related kinase 10 (NEK10), transcript variant X17, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017005771.2

ORF Insert Sequence:

ATGCCTGATC AAGATAAAAA GGTGAAGACC ACAGAAAAAT CAACTGATAA ACAGCAAGAA 
ATCACCATCA GGGACTATTC AGATCTTAAA AGACTTCGGT GCCTTTTGAA CGTCCAATCA 
AGCAAACAAC AGCTTCCAGC CATTAACTTC GATAGTGCCC AAAATAGCAT GACGAAGTCT 
GAGCCCGCCA TCAGGGCGGG TGGACACAGA GCTCGGGGTC AGTGGCATGA ATCCACAGAA 
GCTGTTGAAC TTGAAAATTT TAGTATAAAC TACAAGAATG AGAGAAATTT CAGCAAACAT 
CCTCAGCGTA AACTATTTCA GGAGATCTTT ACCGCCTTGG TGAAAAATAG ACTCATAAGC 
AGAGAGTGGG TTAATCGAGC CCCATCTATT CATTTTCTGA GAGTGTTAAT CTGTCTGAGG 
CTACTAATGA GGGATCCATG TTATCAGGAA ATACTCCATA GCTTGGGTGG GATTGAAAAC 
CTAGCTCAGT ATATGGAGAT TGTAGCCAAT GAGTACCTCG GCTATGGAGA AGAGCAGCAC 
ACTGTGGACA AGCTGGTCAA CATGACATAT ATTTTTCAAA AACTTGCTGC AGTCAAAGAT 
CAAAGAGAAT GGGTCACCAC AAGTGGAGCC CACAAGACAT TAGTAAATTT ACTTGGTGCC 
CGAGATACTA ATGTTCTATT GGGTTCCCTT CTGGCTCTGG CTAGTTTAGC AGAAAGTCAA 
GAATGTAGGG AGAAGATAAG TGAACTCAAC ATTGTAGAAA ATCTGTTGAT GATTTTACAT 
GAATATGACT TGCTTTCTAA AAGACTAACA GCGGAGTTGC TGCGCCTACT TTGTGCAGAG 
CCCCAGGTGA AAGAGCAGGT GAAGCTCTAT GAGGGGATAC CGGTCCTCCT CAGTCTGCTC 
CACTCTGACC ACTTGAAGCT CCTCTGGAGC ATTGTCTGGA TTCTGGTACA GGTTTGTGAG 
GACCCTGAGA CCAGCGTGGA AATTCGCATT TGGGGAGGCA TCAAACAGCT TCTTCATATT 
TTACAAGGAG ACAGAAATTT TGTTTCTGAT CACTCCTCCA TTGGAAGCCT GTCCAGTGCA 
AATGCTGCAG GCCGAATCCA GCAGCTTCAT TTATCAGAAG ACTTGAGCCC TAGGGAAATA 
CAAGAAAATA CTTTCTCACT TCAAGCAGCC TGCTGTGCTG CCCTCACTGA GCTGGTGCTC 
AATGACACCA ATGCCCACCA GGTGGTTCAG GAAAATGGTG TATATACAAT AGCAAAATTA 
ATTTTACCAA ATAAGCAAAA GAATGCAGCA AAAAGTAATC TATTACAGTG TTATGCTTTC 
AGAGCCTTGA GATTTCTCTT CAGTATGGAA AGAAACAGAC CACTCTTTAA AAGACTTTTC 
CCCACAGACT TGTTTGAGAT CTTCATTGAC ATAGGGCATT ATGTACGTGA TATCAGTGCT 
TATGAAGAAT TGGTATCCAA GCTGAATTTA TTAGTGGAGG ATGAACTGAA GCAAATTGCT 
GAAAATATTG AAAGCATTAA TCAGAACAAA GCTCCTTTGA AATATATAGG CAACTATGCA 
ATTTTGGATC ATCTTGGAAG TGGAGCTTTT GGCTGTGTTT ACAAGGTTAG AAAGCATAGT 
GGTCAAAATC TTTTAGCAAT GAAAGAGGTC AATTTACATA ACCCAGCATT TGGAAAGGAT 
AAGAAAGATC GAGACAGCAG CGTAAGGAAT ATTGTTTCTG AATTAACAAT AATTAAAGAG 
CAGCTTTATC ATCCCAACAT TGTACGTTAT TACAAAACAT TTCTGGAAAA TGATAGGTTG 
TACATAGTTA TGGAGCTGAT AGAAGGAGCC CCGCTTGGAG AGCATTTCAG TTCTTTGAAG 
GAAAAACATC ACCATTTTAC TGAAGAAAGA CTATGGAAAA TATTTATACA GCTGTGCTTA 
GCTCTTCGAT ACTTACACAA GGAGAAGAGG ATTGTCCATA GAGATCTGAC ACCAAACAAC 
ATTATGTTGG GGGATAAGGA CAAAGTAACC GTTACTGACT TTGGCCTGGC AAAGCAAAAA 
CAAGAAAACA GTAAACTCAC CTCTGTGGTT GGAACAATCC TGTATTCTTG CCCCGAGGTA 
CTGAAGAGTG AGCCGTATGG GGAGAAGGCT GATGTCTGGG CAGTAGGCTG CATCCTTTAT 
CAGATGGCGA CTTTGAGTCC CCCCTTCTAC AGCACTAACA TGCTGTCCTT GGCTACAAAA 
ATAGTGGAGG CGGTATATGA ACCAGTCCCA GAAGGTATCT ACTCTGAAAA AGTAACAGAC 
ACCATCAGCA GGTGCCTCAC TCCTGATGCG GAAGCTCGTC CAGATATTGT AGAAGTCAGT 
TCGATGATAT CAGATGTCAT GATGAAATAT TTAGACAACT TATCTACATC CCAGTTGTCC 
TTGGAAAAGA AGCTAGAACG GGAACGAAGA CGCACACAAA GGTATTTTAT GGAAGCCAAC 
CGGAACACCG TCACATGTCA CCATGAGCTG GCTGTTCTAT CTCACGAGAC CTTTGAGAAG 
GCAAGTTTGA GTAGCAGCAG CAGTGGAGCA GCCAGCCTGA AAAGTGAACT TTCAGAAAGC 
GCAGACCTGC CCCCTGAAGG CTTCCAGGCC TCCTATGGTA AAGACGAAGA CAGGGCCTGT 
GACGAAATCC TGTCAGATGA TAACTTCAAC CTGGAAAATG CTGAGAAAGA TACATATTCA 
GAGGTAGATG ATGAATTGGA CATTTCGGAT AACTCCAGCA GCTCCAGTTC AAGCCCTCTG 
AAAGAATCTA CATTCAACAT TTTAAAGAGA AGTTTTAGTG CTTCAGGAGG AGAAAGACAA 
TCCCAAACAA GGGACTTCAC TGGAGGAACA GGATCAAGAC CAAGACCAGC ATCAGCAGGA 
ATTGCTGTGT CCCAGAGGAA AGTGCGTCAG ATCAGTGATC CTATTCAGCA GATATTAATT 
CAGCTGCACA AAATAATCTA TATCACACAG CTTCCTCCAG CTTTGCACCA CAATTTGAAA 
AGAAGGGTTA TAGAGAGATT CAAGAAATCC CTCTTCAGCC AGCAGAGTAA CCCTTGTAAT 
TTGAAATCTG AAATTAAAAA GTTATCTCAG GGATCTCCAG AACCGATTGA GCCCAACTTT 
TTCACAGCAG ATTACCATTT ATTACATCGT TCATCCGGTG GAAACAGCCT GTCCCCAAAT 
GACCCTACAG GTTTACCAAC CAGCATTGAA TTGGAGGAAG GAATAACATA TGAACAGATG 
CAGACTGTGA TTGAAGAAGT CCTTGAGGAA AGTGGCTATT ACAATTTTAC ATCTAACAGG 
TATCATTCCT ATCCATGGGG GACCAAGAAT CACCCAACCA AAAGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Nek10 mediates G2/M cell cycle arrest and MEK autoactivation in response to UV irradiation.
Molecular and cellular biology31(1)30-42(2011 Jan)
Moniz LS,Stambolic V

Newly discovered breast cancer susceptibility loci on 3p24 and 17q23.2.
Nature genetics41(5)585-90(2009 May)
Ahmed S,Thomas G,Ghoussaini M,Healey CS,Humphreys MK,Platte R,Morrison J,Maranian M,Pooley KA,Luben R,Eccles D,Evans DG,Fletcher O,Johnson N,dos Santos Silva I,Peto J,Stratton MR,Rahman N,Jacobs K,Prentice R,Anderson GL,Rajkovic A,Curb JD,Ziegler RG,Berg CD,Buys SS,McCarty CA,Feigelson HS,Calle EE,Thun MJ,Diver WR,Bojesen S,Nordestgaard BG,Flyger H,D?rk T,Sch?rmann P,Hillemanns P,Karstens JH,Bogdanova NV,Antonenkova NN,Zalutsky IV,Bermisheva M,Fedorova S,Khusnutdinova E,,Kang D,Yoo KY,Noh DY,Ahn SH,Devilee P,van Asperen CJ,Tollenaar RA,Seynaeve C,Garcia-Closas M,Lissowska J,Brinton L,Peplonska B,Nevanlinna H,Heikkinen T,Aittom?ki K,Blomqvist C,Hopper JL,Southey MC,Smith L,Spurdle AB,Schmidt MK,Broeks A,van Hien RR,Cornelissen S,Milne RL,Ribas G,Gonz?lez-Neira A,Benitez J,Schmutzler RK,Burwinkel B,Bartram CR,Meindl A,Brauch H,Justenhoven C,Hamann U,,Chang-Claude J,Hein R,Wang-Gohrke S,Lindblom A,Margolin S,Mannermaa A,Kosma VM,Kataja V,Olson JE,Wang X,Fredericksen Z,Giles GG,Severi G,Baglietto L,English DR,Hankinson SE,Cox DG,Kraft P,Vatten LJ,Hveem K,Kumle M,Sigurdson A,Doody M,Bhatti P,Alexander BH,Hooning MJ,van den Ouweland AM,Oldenburg RA,Schutte M,Hall P,Czene K,Liu J,Li Y,Cox A,Elliott G,Brock I,Reed MW,Shen CY,Yu JC,Hsu GC,Chen ST,Anton-Culver H,Ziogas A,Andrulis IL,Knight JA,,,Beesley J,Goode EL,Couch F,Chenevix-Trench G,Hoover RN,Ponder BA,Hunter DJ,Pharoah PD,Dunning AM,Chanock SJ,Easton DF

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Pan troglodytes (chimpanzee)	NEK10	XP_001165108.1
Homo sapiens (human)	NEK10	NP_955379.2

NEK10 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

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