DMPK cDNA ORF clone, Homo sapiens(human)

The following DMPK gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the DMPK cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

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***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OHu20642 NM_004409.5
Latest version!
Homo sapiens DM1 protein kinase (DMPK), transcript variant 2, mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK
$189.50-$265.30
$379.00
OHu20985 NM_001081560.3
Latest version!
Homo sapiens DM1 protein kinase (DMPK), transcript variant 3, mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK
$49.50-$69.30
$99.00
OHu21523 NM_001081562.3
Latest version!
Homo sapiens DM1 protein kinase (DMPK), transcript variant 4, mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK
$49.50-$69.30
$99.00
OHu21765 NM_001081563.2
Latest version!
Homo sapiens DM1 protein kinase (DMPK), transcript variant 1, mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK
$49.50-$69.30
$99.00
OHu20680 NM_001288766.2
Latest version!
Homo sapiens DM1 protein kinase (DMPK), transcript variant 7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $307.30
$439.00
OHu20965 NM_001288765.1
Latest version!
Homo sapiens DM1 protein kinase (DMPK), transcript variant 6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $307.30
$439.00
OHu21375 NM_001288764.2
Latest version!
Homo sapiens DM1 protein kinase (DMPK), transcript variant 5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $307.30
$439.00
View Old Accession Versions >>
OHu21523 NM_001081562.2 Homo sapiens DM1 protein kinase (DMPK), transcript variant 4, mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK
$49.50-$69.30
$99.00
OHu20985 NM_001081560.2 Homo sapiens DM1 protein kinase (DMPK), transcript variant 3, mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK
$49.50-$69.30
$99.00
OHu21375 NM_001288764.1 Homo sapiens DM1 protein kinase (DMPK), transcript variant 5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $307.30
$439.00
OHu20642 NM_004409.4 Homo sapiens DM1 protein kinase (DMPK), transcript variant 2, mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK
$189.50-$265.30
$379.00
OHu20680 NM_001288766.1 Homo sapiens DM1 protein kinase (DMPK), transcript variant 7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $307.30
$439.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OHu20642
    Clone ID Related Accession (Same CDS sequence) NM_004409.4 , NM_004409.5
    Accession Version NM_004409.4 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1890bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-03-11
    Organism Homo sapiens(human)
    Product myotonin-protein kinase isoform 2
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC011530.6, BG706056.1, BC062553.1, AV655848.1 and AC074212.5. This sequence is a reference standard in the RefSeqGene project. On Jan 4, 2014 this sequence version replaced NM_004409.3. Transcript Variant: This variant (2) has multiple differences in the presence and absence of exons at its 5' end, compared to variant 1. These differences produce a distinct 5' UTR and cause translation initiation at an alternative start codon, compared to variant 1. The encoded protein (isoform 2, also known as isoform 9) has a distinct N-terminus and is shorter than isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC062553.1, SRR1803613.74233.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    ATGTCAGCCG AGGTGCGGCT GAGGCGGCTC CAGCAGCTGG TGTTGGACCC GGGCTTCCTG 
    GGGCTGGAGC CCCTGCTCGA CCTTCTCCTG GGCGTCCACC AGGAGCTGGG CGCCTCCGAA
    CTGGCCCAGG ACAAGTACGT GGCCGACTTC TTGCAGTGGG CGGAGCCCAT CGTGGTGAGG
    CTTAAGGAGG TCCGACTGCA GAGGGACGAC TTCGAGATTC TGAAGGTGAT CGGACGCGGG
    GCGTTCAGCG AGGTAGCGGT AGTGAAGATG AAGCAGACGG GCCAGGTGTA TGCCATGAAG
    ATCATGAACA AGTGGGACAT GCTGAAGAGG GGCGAGGTGT CGTGCTTCCG TGAGGAGAGG
    GACGTGTTGG TGAATGGGGA CCGGCGGTGG ATCACGCAGC TGCACTTCGC CTTCCAGGAT
    GAGAACTACC TGTACCTGGT CATGGAGTAT TACGTGGGCG GGGACCTGCT GACACTGCTG
    AGCAAGTTTG GGGAGCGGAT TCCGGCCGAG ATGGCGCGCT TCTACCTGGC GGAGATTGTC
    ATGGCCATAG ACTCGGTGCA CCGGCTTGGC TACGTGCACA GGGACATCAA ACCCGACAAC
    ATCCTGCTGG ACCGCTGTGG CCACATCCGC CTGGCCGACT TCGGCTCTTG CCTCAAGCTG
    CGGGCAGATG GAACGGTGCG GTCGCTGGTG GCTGTGGGCA CCCCAGACTA CCTGTCCCCC
    GAGATCCTGC AGGCTGTGGG CGGTGGGCCT GGGACAGGCA GCTACGGGCC CGAGTGTGAC
    TGGTGGGCGC TGGGTGTATT CGCCTATGAA ATGTTCTATG GGCAGACGCC CTTCTACGCG
    GATTCCACGG CGGAGACCTA TGGCAAGATC GTCCACTACA AGGAGCACCT CTCTCTGCCG
    CTGGTGGACG AAGGGGTCCC TGAGGAGGCT CGAGACTTCA TTCAGCGGTT GCTGTGTCCC
    CCGGAGACAC GGCTGGGCCG GGGTGGAGCA GGCGACTTCC GGACACATCC CTTCTTCTTT
    GGCCTCGACT GGGATGGTCT CCGGGACAGC GTGCCCCCCT TTACACCGGA TTTCGAAGGT
    GCCACCGACA CATGCAACTT CGACTTGGTG GAGGACGGGC TCACTGCCAT GGTGAGCGGG
    GGCGGGGAGA CACTGTCGGA CATTCGGGAA GGTGCGCCGC TAGGGGTCCA CCTGCCTTTT
    GTGGGCTACT CCTACTCCTG CATGGCCCTC AGGGACAGTG AGGTCCCAGG CCCCACACCC
    ATGGAACTGG AGGCCGAGCA GCTGCTTGAG CCACACGTGC AAGCGCCCAG CCTGGAGCCC
    TCGGTGTCCC CACAGGATGA AACAGCTGAA GTGGCAGTTC CAGCGGCTGT CCCTGCGGCA
    GAGGCTGAGG CCGAGGTGAC GCTGCGGGAG CTCCAGGAAG CCCTGGAGGA GGAGGTGCTC
    ACCCGGCAGA GCCTGAGCCG GGAGATGGAG GCCATCCGCA CGGACAACCA GAACTTCGCC
    AGTCAACTAC GCGAGGCAGA GGCTCGGAAC CGGGACCTAG AGGCACACGT CCGGCAGTTG
    CAGGAGCGGA TGGAGTTGCT GCAGGCAGAG GGAGCCACAG CTGTCACGGG GGTCCCCAGT
    CCCCGGGCCA CGGATCCACC TTCCCATCTA GATGGCCCCC CGGCCGTGGC TGTGGGCCAG
    TGCCCGCTGG TGGGGCCAGG CCCCATGCAC CGCCGCCACC TGCTGCTCCC TGCCAGGGTC
    CCTAGGCCTG GCCTATCGGA GGCGCTTTCC CTGCTCCTGT TCGCCGTTGT TCTGTCTCGT
    GCCGCCGCCC TGGGCTGCAT TGGGTTGGTG GCCCACGCCG GCCAACTCAC CGCAGTCTGG
    CGCCGCCCAG GAGCCGCCCG CGCTCCCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_004400.4
    CDS206..2095
    Translation

    Target ORF information:

    RefSeq Version NM_004409.4
    Organism Homo sapiens(human)
    Definition Homo sapiens DM1 protein kinase (DMPK), transcript variant 2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_004409.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    ATGTCAGCCG AGGTGCGGCT GAGGCGGCTC CAGCAGCTGG TGTTGGACCC GGGCTTCCTG 
    GGGCTGGAGC CCCTGCTCGA CCTTCTCCTG GGCGTCCACC AGGAGCTGGG CGCCTCCGAA
    CTGGCCCAGG ACAAGTACGT GGCCGACTTC TTGCAGTGGG CGGAGCCCAT CGTGGTGAGG
    CTTAAGGAGG TCCGACTGCA GAGGGACGAC TTCGAGATTC TGAAGGTGAT CGGACGCGGG
    GCGTTCAGCG AGGTAGCGGT AGTGAAGATG AAGCAGACGG GCCAGGTGTA TGCCATGAAG
    ATCATGAACA AGTGGGACAT GCTGAAGAGG GGCGAGGTGT CGTGCTTCCG TGAGGAGAGG
    GACGTGTTGG TGAATGGGGA CCGGCGGTGG ATCACGCAGC TGCACTTCGC CTTCCAGGAT
    GAGAACTACC TGTACCTGGT CATGGAGTAT TACGTGGGCG GGGACCTGCT GACACTGCTG
    AGCAAGTTTG GGGAGCGGAT TCCGGCCGAG ATGGCGCGCT TCTACCTGGC GGAGATTGTC
    ATGGCCATAG ACTCGGTGCA CCGGCTTGGC TACGTGCACA GGGACATCAA ACCCGACAAC
    ATCCTGCTGG ACCGCTGTGG CCACATCCGC CTGGCCGACT TCGGCTCTTG CCTCAAGCTG
    CGGGCAGATG GAACGGTGCG GTCGCTGGTG GCTGTGGGCA CCCCAGACTA CCTGTCCCCC
    GAGATCCTGC AGGCTGTGGG CGGTGGGCCT GGGACAGGCA GCTACGGGCC CGAGTGTGAC
    TGGTGGGCGC TGGGTGTATT CGCCTATGAA ATGTTCTATG GGCAGACGCC CTTCTACGCG
    GATTCCACGG CGGAGACCTA TGGCAAGATC GTCCACTACA AGGAGCACCT CTCTCTGCCG
    CTGGTGGACG AAGGGGTCCC TGAGGAGGCT CGAGACTTCA TTCAGCGGTT GCTGTGTCCC
    CCGGAGACAC GGCTGGGCCG GGGTGGAGCA GGCGACTTCC GGACACATCC CTTCTTCTTT
    GGCCTCGACT GGGATGGTCT CCGGGACAGC GTGCCCCCCT TTACACCGGA TTTCGAAGGT
    GCCACCGACA CATGCAACTT CGACTTGGTG GAGGACGGGC TCACTGCCAT GGTGAGCGGG
    GGCGGGGAGA CACTGTCGGA CATTCGGGAA GGTGCGCCGC TAGGGGTCCA CCTGCCTTTT
    GTGGGCTACT CCTACTCCTG CATGGCCCTC AGGGACAGTG AGGTCCCAGG CCCCACACCC
    ATGGAACTGG AGGCCGAGCA GCTGCTTGAG CCACACGTGC AAGCGCCCAG CCTGGAGCCC
    TCGGTGTCCC CACAGGATGA AACAGCTGAA GTGGCAGTTC CAGCGGCTGT CCCTGCGGCA
    GAGGCTGAGG CCGAGGTGAC GCTGCGGGAG CTCCAGGAAG CCCTGGAGGA GGAGGTGCTC
    ACCCGGCAGA GCCTGAGCCG GGAGATGGAG GCCATCCGCA CGGACAACCA GAACTTCGCC
    AGTCAACTAC GCGAGGCAGA GGCTCGGAAC CGGGACCTAG AGGCACACGT CCGGCAGTTG
    CAGGAGCGGA TGGAGTTGCT GCAGGCAGAG GGAGCCACAG CTGTCACGGG GGTCCCCAGT
    CCCCGGGCCA CGGATCCACC TTCCCATCTA GATGGCCCCC CGGCCGTGGC TGTGGGCCAG
    TGCCCGCTGG TGGGGCCAGG CCCCATGCAC CGCCGCCACC TGCTGCTCCC TGCCAGGGTC
    CCTAGGCCTG GCCTATCGGA GGCGCTTTCC CTGCTCCTGT TCGCCGTTGT TCTGTCTCGT
    GCCGCCGCCC TGGGCTGCAT TGGGTTGGTG GCCCACGCCG GCCAACTCAC CGCAGTCTGG
    CGCCGCCCAG GAGCCGCCCG CGCTCCCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu20642
    Clone ID Related Accession (Same CDS sequence) NM_004409.4 , NM_004409.5
    Accession Version NM_004409.5 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1890bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2020-01-04
    Organism Homo sapiens(human)
    Product myotonin-protein kinase isoform 2
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BG706056.1, BC062553.1 and AV655848.1. This sequence is a reference standard in the RefSeqGene project. On Jun 2, 2019 this sequence version replaced NM_004409.4. Transcript Variant: This variant (2) has multiple differences in the presence and absence of exons at its 5' end, compared to variant 1. These differences produce a distinct 5' UTR and cause translation initiation at an alternative start codon, compared to variant 1. The encoded protein (isoform 2, also known as isoform 9) has a distinct N-terminus and is shorter than isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803613.74233.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## RefSeq Select criteria :: based on manual assertion, expression ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    ATGTCAGCCG AGGTGCGGCT GAGGCGGCTC CAGCAGCTGG TGTTGGACCC GGGCTTCCTG 
    GGGCTGGAGC CCCTGCTCGA CCTTCTCCTG GGCGTCCACC AGGAGCTGGG CGCCTCCGAA
    CTGGCCCAGG ACAAGTACGT GGCCGACTTC TTGCAGTGGG CGGAGCCCAT CGTGGTGAGG
    CTTAAGGAGG TCCGACTGCA GAGGGACGAC TTCGAGATTC TGAAGGTGAT CGGACGCGGG
    GCGTTCAGCG AGGTAGCGGT AGTGAAGATG AAGCAGACGG GCCAGGTGTA TGCCATGAAG
    ATCATGAACA AGTGGGACAT GCTGAAGAGG GGCGAGGTGT CGTGCTTCCG TGAGGAGAGG
    GACGTGTTGG TGAATGGGGA CCGGCGGTGG ATCACGCAGC TGCACTTCGC CTTCCAGGAT
    GAGAACTACC TGTACCTGGT CATGGAGTAT TACGTGGGCG GGGACCTGCT GACACTGCTG
    AGCAAGTTTG GGGAGCGGAT TCCGGCCGAG ATGGCGCGCT TCTACCTGGC GGAGATTGTC
    ATGGCCATAG ACTCGGTGCA CCGGCTTGGC TACGTGCACA GGGACATCAA ACCCGACAAC
    ATCCTGCTGG ACCGCTGTGG CCACATCCGC CTGGCCGACT TCGGCTCTTG CCTCAAGCTG
    CGGGCAGATG GAACGGTGCG GTCGCTGGTG GCTGTGGGCA CCCCAGACTA CCTGTCCCCC
    GAGATCCTGC AGGCTGTGGG CGGTGGGCCT GGGACAGGCA GCTACGGGCC CGAGTGTGAC
    TGGTGGGCGC TGGGTGTATT CGCCTATGAA ATGTTCTATG GGCAGACGCC CTTCTACGCG
    GATTCCACGG CGGAGACCTA TGGCAAGATC GTCCACTACA AGGAGCACCT CTCTCTGCCG
    CTGGTGGACG AAGGGGTCCC TGAGGAGGCT CGAGACTTCA TTCAGCGGTT GCTGTGTCCC
    CCGGAGACAC GGCTGGGCCG GGGTGGAGCA GGCGACTTCC GGACACATCC CTTCTTCTTT
    GGCCTCGACT GGGATGGTCT CCGGGACAGC GTGCCCCCCT TTACACCGGA TTTCGAAGGT
    GCCACCGACA CATGCAACTT CGACTTGGTG GAGGACGGGC TCACTGCCAT GGTGAGCGGG
    GGCGGGGAGA CACTGTCGGA CATTCGGGAA GGTGCGCCGC TAGGGGTCCA CCTGCCTTTT
    GTGGGCTACT CCTACTCCTG CATGGCCCTC AGGGACAGTG AGGTCCCAGG CCCCACACCC
    ATGGAACTGG AGGCCGAGCA GCTGCTTGAG CCACACGTGC AAGCGCCCAG CCTGGAGCCC
    TCGGTGTCCC CACAGGATGA AACAGCTGAA GTGGCAGTTC CAGCGGCTGT CCCTGCGGCA
    GAGGCTGAGG CCGAGGTGAC GCTGCGGGAG CTCCAGGAAG CCCTGGAGGA GGAGGTGCTC
    ACCCGGCAGA GCCTGAGCCG GGAGATGGAG GCCATCCGCA CGGACAACCA GAACTTCGCC
    AGTCAACTAC GCGAGGCAGA GGCTCGGAAC CGGGACCTAG AGGCACACGT CCGGCAGTTG
    CAGGAGCGGA TGGAGTTGCT GCAGGCAGAG GGAGCCACAG CTGTCACGGG GGTCCCCAGT
    CCCCGGGCCA CGGATCCACC TTCCCATCTA GATGGCCCCC CGGCCGTGGC TGTGGGCCAG
    TGCCCGCTGG TGGGGCCAGG CCCCATGCAC CGCCGCCACC TGCTGCTCCC TGCCAGGGTC
    CCTAGGCCTG GCCTATCGGA GGCGCTTTCC CTGCTCCTGT TCGCCGTTGT TCTGTCTCGT
    GCCGCCGCCC TGGGCTGCAT TGGGTTGGTG GCCCACGCCG GCCAACTCAC CGCAGTCTGG
    CGCCGCCCAG GAGCCGCCCG CGCTCCCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_004400.4
    CDS139..2028
    Translation

    Target ORF information:

    RefSeq Version NM_004409.5
    Organism Homo sapiens(human)
    Definition Homo sapiens DM1 protein kinase (DMPK), transcript variant 2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_004409.5

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    ATGTCAGCCG AGGTGCGGCT GAGGCGGCTC CAGCAGCTGG TGTTGGACCC GGGCTTCCTG 
    GGGCTGGAGC CCCTGCTCGA CCTTCTCCTG GGCGTCCACC AGGAGCTGGG CGCCTCCGAA
    CTGGCCCAGG ACAAGTACGT GGCCGACTTC TTGCAGTGGG CGGAGCCCAT CGTGGTGAGG
    CTTAAGGAGG TCCGACTGCA GAGGGACGAC TTCGAGATTC TGAAGGTGAT CGGACGCGGG
    GCGTTCAGCG AGGTAGCGGT AGTGAAGATG AAGCAGACGG GCCAGGTGTA TGCCATGAAG
    ATCATGAACA AGTGGGACAT GCTGAAGAGG GGCGAGGTGT CGTGCTTCCG TGAGGAGAGG
    GACGTGTTGG TGAATGGGGA CCGGCGGTGG ATCACGCAGC TGCACTTCGC CTTCCAGGAT
    GAGAACTACC TGTACCTGGT CATGGAGTAT TACGTGGGCG GGGACCTGCT GACACTGCTG
    AGCAAGTTTG GGGAGCGGAT TCCGGCCGAG ATGGCGCGCT TCTACCTGGC GGAGATTGTC
    ATGGCCATAG ACTCGGTGCA CCGGCTTGGC TACGTGCACA GGGACATCAA ACCCGACAAC
    ATCCTGCTGG ACCGCTGTGG CCACATCCGC CTGGCCGACT TCGGCTCTTG CCTCAAGCTG
    CGGGCAGATG GAACGGTGCG GTCGCTGGTG GCTGTGGGCA CCCCAGACTA CCTGTCCCCC
    GAGATCCTGC AGGCTGTGGG CGGTGGGCCT GGGACAGGCA GCTACGGGCC CGAGTGTGAC
    TGGTGGGCGC TGGGTGTATT CGCCTATGAA ATGTTCTATG GGCAGACGCC CTTCTACGCG
    GATTCCACGG CGGAGACCTA TGGCAAGATC GTCCACTACA AGGAGCACCT CTCTCTGCCG
    CTGGTGGACG AAGGGGTCCC TGAGGAGGCT CGAGACTTCA TTCAGCGGTT GCTGTGTCCC
    CCGGAGACAC GGCTGGGCCG GGGTGGAGCA GGCGACTTCC GGACACATCC CTTCTTCTTT
    GGCCTCGACT GGGATGGTCT CCGGGACAGC GTGCCCCCCT TTACACCGGA TTTCGAAGGT
    GCCACCGACA CATGCAACTT CGACTTGGTG GAGGACGGGC TCACTGCCAT GGTGAGCGGG
    GGCGGGGAGA CACTGTCGGA CATTCGGGAA GGTGCGCCGC TAGGGGTCCA CCTGCCTTTT
    GTGGGCTACT CCTACTCCTG CATGGCCCTC AGGGACAGTG AGGTCCCAGG CCCCACACCC
    ATGGAACTGG AGGCCGAGCA GCTGCTTGAG CCACACGTGC AAGCGCCCAG CCTGGAGCCC
    TCGGTGTCCC CACAGGATGA AACAGCTGAA GTGGCAGTTC CAGCGGCTGT CCCTGCGGCA
    GAGGCTGAGG CCGAGGTGAC GCTGCGGGAG CTCCAGGAAG CCCTGGAGGA GGAGGTGCTC
    ACCCGGCAGA GCCTGAGCCG GGAGATGGAG GCCATCCGCA CGGACAACCA GAACTTCGCC
    AGTCAACTAC GCGAGGCAGA GGCTCGGAAC CGGGACCTAG AGGCACACGT CCGGCAGTTG
    CAGGAGCGGA TGGAGTTGCT GCAGGCAGAG GGAGCCACAG CTGTCACGGG GGTCCCCAGT
    CCCCGGGCCA CGGATCCACC TTCCCATCTA GATGGCCCCC CGGCCGTGGC TGTGGGCCAG
    TGCCCGCTGG TGGGGCCAGG CCCCATGCAC CGCCGCCACC TGCTGCTCCC TGCCAGGGTC
    CCTAGGCCTG GCCTATCGGA GGCGCTTTCC CTGCTCCTGT TCGCCGTTGT TCTGTCTCGT
    GCCGCCGCCC TGGGCTGCAT TGGGTTGGTG GCCCACGCCG GCCAACTCAC CGCAGTCTGG
    CGCCGCCCAG GAGCCGCCCG CGCTCCCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu20985
    Clone ID Related Accession (Same CDS sequence) NM_001081560.2 , NM_001081560.3
    Accession Version NM_001081560.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1875bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-03-11
    Organism Homo sapiens(human)
    Product myotonin-protein kinase isoform 3
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC011530.6, L19268.1, BC062553.1, AV655848.1 and AC074212.5. On Jan 4, 2014 this sequence version replaced NM_001081560.1. Transcript Variant: This variant (3) has multiple differences in the presence and absence of exons at its 5' end and in the CDS, compared to variant 1. These differences produce a distinct 5' UTR, cause translation initiation at an alternative start codon, and the loss of an in-frame portion of the coding region, compared to variant 1. The encoded protein (isoform 3, also known as isoform 11) has a distinct N-terminus and is shorter than isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: L19268.1, M94203.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    ATGTCAGCCG AGGTGCGGCT GAGGCGGCTC CAGCAGCTGG TGTTGGACCC GGGCTTCCTG 
    GGGCTGGAGC CCCTGCTCGA CCTTCTCCTG GGCGTCCACC AGGAGCTGGG CGCCTCCGAA
    CTGGCCCAGG ACAAGTACGT GGCCGACTTC TTGCAGTGGG CGGAGCCCAT CGTGGTGAGG
    CTTAAGGAGG TCCGACTGCA GAGGGACGAC TTCGAGATTC TGAAGGTGAT CGGACGCGGG
    GCGTTCAGCG AGGTAGCGGT AGTGAAGATG AAGCAGACGG GCCAGGTGTA TGCCATGAAG
    ATCATGAACA AGTGGGACAT GCTGAAGAGG GGCGAGGTGT CGTGCTTCCG TGAGGAGAGG
    GACGTGTTGG TGAATGGGGA CCGGCGGTGG ATCACGCAGC TGCACTTCGC CTTCCAGGAT
    GAGAACTACC TGTACCTGGT CATGGAGTAT TACGTGGGCG GGGACCTGCT GACACTGCTG
    AGCAAGTTTG GGGAGCGGAT TCCGGCCGAG ATGGCGCGCT TCTACCTGGC GGAGATTGTC
    ATGGCCATAG ACTCGGTGCA CCGGCTTGGC TACGTGCACA GGGACATCAA ACCCGACAAC
    ATCCTGCTGG ACCGCTGTGG CCACATCCGC CTGGCCGACT TCGGCTCTTG CCTCAAGCTG
    CGGGCAGATG GAACGGTGCG GTCGCTGGTG GCTGTGGGCA CCCCAGACTA CCTGTCCCCC
    GAGATCCTGC AGGCTGTGGG CGGTGGGCCT GGGACAGGCA GCTACGGGCC CGAGTGTGAC
    TGGTGGGCGC TGGGTGTATT CGCCTATGAA ATGTTCTATG GGCAGACGCC CTTCTACGCG
    GATTCCACGG CGGAGACCTA TGGCAAGATC GTCCACTACA AGGAGCACCT CTCTCTGCCG
    CTGGTGGACG AAGGGGTCCC TGAGGAGGCT CGAGACTTCA TTCAGCGGTT GCTGTGTCCC
    CCGGAGACAC GGCTGGGCCG GGGTGGAGCA GGCGACTTCC GGACACATCC CTTCTTCTTT
    GGCCTCGACT GGGATGGTCT CCGGGACAGC GTGCCCCCCT TTACACCGGA TTTCGAAGGT
    GCCACCGACA CATGCAACTT CGACTTGGTG GAGGACGGGC TCACTGCCAT GGAGACACTG
    TCGGACATTC GGGAAGGTGC GCCGCTAGGG GTCCACCTGC CTTTTGTGGG CTACTCCTAC
    TCCTGCATGG CCCTCAGGGA CAGTGAGGTC CCAGGCCCCA CACCCATGGA ACTGGAGGCC
    GAGCAGCTGC TTGAGCCACA CGTGCAAGCG CCCAGCCTGG AGCCCTCGGT GTCCCCACAG
    GATGAAACAG CTGAAGTGGC AGTTCCAGCG GCTGTCCCTG CGGCAGAGGC TGAGGCCGAG
    GTGACGCTGC GGGAGCTCCA GGAAGCCCTG GAGGAGGAGG TGCTCACCCG GCAGAGCCTG
    AGCCGGGAGA TGGAGGCCAT CCGCACGGAC AACCAGAACT TCGCCAGTCA ACTACGCGAG
    GCAGAGGCTC GGAACCGGGA CCTAGAGGCA CACGTCCGGC AGTTGCAGGA GCGGATGGAG
    TTGCTGCAGG CAGAGGGAGC CACAGCTGTC ACGGGGGTCC CCAGTCCCCG GGCCACGGAT
    CCACCTTCCC ATCTAGATGG CCCCCCGGCC GTGGCTGTGG GCCAGTGCCC GCTGGTGGGG
    CCAGGCCCCA TGCACCGCCG CCACCTGCTG CTCCCTGCCA GGGTCCCTAG GCCTGGCCTA
    TCGGAGGCGC TTTCCCTGCT CCTGTTCGCC GTTGTTCTGT CTCGTGCCGC CGCCCTGGGC
    TGCATTGGGT TGGTGGCCCA CGCCGGCCAA CTCACCGCAG TCTGGCGCCG CCCAGGAGCC
    GCCCGCGCTC CCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001075029.1
    CDS206..2080
    Translation

    Target ORF information:

    RefSeq Version NM_001081560.2
    Organism Homo sapiens(human)
    Definition Homo sapiens DM1 protein kinase (DMPK), transcript variant 3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001081560.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    ATGTCAGCCG AGGTGCGGCT GAGGCGGCTC CAGCAGCTGG TGTTGGACCC GGGCTTCCTG 
    GGGCTGGAGC CCCTGCTCGA CCTTCTCCTG GGCGTCCACC AGGAGCTGGG CGCCTCCGAA
    CTGGCCCAGG ACAAGTACGT GGCCGACTTC TTGCAGTGGG CGGAGCCCAT CGTGGTGAGG
    CTTAAGGAGG TCCGACTGCA GAGGGACGAC TTCGAGATTC TGAAGGTGAT CGGACGCGGG
    GCGTTCAGCG AGGTAGCGGT AGTGAAGATG AAGCAGACGG GCCAGGTGTA TGCCATGAAG
    ATCATGAACA AGTGGGACAT GCTGAAGAGG GGCGAGGTGT CGTGCTTCCG TGAGGAGAGG
    GACGTGTTGG TGAATGGGGA CCGGCGGTGG ATCACGCAGC TGCACTTCGC CTTCCAGGAT
    GAGAACTACC TGTACCTGGT CATGGAGTAT TACGTGGGCG GGGACCTGCT GACACTGCTG
    AGCAAGTTTG GGGAGCGGAT TCCGGCCGAG ATGGCGCGCT TCTACCTGGC GGAGATTGTC
    ATGGCCATAG ACTCGGTGCA CCGGCTTGGC TACGTGCACA GGGACATCAA ACCCGACAAC
    ATCCTGCTGG ACCGCTGTGG CCACATCCGC CTGGCCGACT TCGGCTCTTG CCTCAAGCTG
    CGGGCAGATG GAACGGTGCG GTCGCTGGTG GCTGTGGGCA CCCCAGACTA CCTGTCCCCC
    GAGATCCTGC AGGCTGTGGG CGGTGGGCCT GGGACAGGCA GCTACGGGCC CGAGTGTGAC
    TGGTGGGCGC TGGGTGTATT CGCCTATGAA ATGTTCTATG GGCAGACGCC CTTCTACGCG
    GATTCCACGG CGGAGACCTA TGGCAAGATC GTCCACTACA AGGAGCACCT CTCTCTGCCG
    CTGGTGGACG AAGGGGTCCC TGAGGAGGCT CGAGACTTCA TTCAGCGGTT GCTGTGTCCC
    CCGGAGACAC GGCTGGGCCG GGGTGGAGCA GGCGACTTCC GGACACATCC CTTCTTCTTT
    GGCCTCGACT GGGATGGTCT CCGGGACAGC GTGCCCCCCT TTACACCGGA TTTCGAAGGT
    GCCACCGACA CATGCAACTT CGACTTGGTG GAGGACGGGC TCACTGCCAT GGAGACACTG
    TCGGACATTC GGGAAGGTGC GCCGCTAGGG GTCCACCTGC CTTTTGTGGG CTACTCCTAC
    TCCTGCATGG CCCTCAGGGA CAGTGAGGTC CCAGGCCCCA CACCCATGGA ACTGGAGGCC
    GAGCAGCTGC TTGAGCCACA CGTGCAAGCG CCCAGCCTGG AGCCCTCGGT GTCCCCACAG
    GATGAAACAG CTGAAGTGGC AGTTCCAGCG GCTGTCCCTG CGGCAGAGGC TGAGGCCGAG
    GTGACGCTGC GGGAGCTCCA GGAAGCCCTG GAGGAGGAGG TGCTCACCCG GCAGAGCCTG
    AGCCGGGAGA TGGAGGCCAT CCGCACGGAC AACCAGAACT TCGCCAGTCA ACTACGCGAG
    GCAGAGGCTC GGAACCGGGA CCTAGAGGCA CACGTCCGGC AGTTGCAGGA GCGGATGGAG
    TTGCTGCAGG CAGAGGGAGC CACAGCTGTC ACGGGGGTCC CCAGTCCCCG GGCCACGGAT
    CCACCTTCCC ATCTAGATGG CCCCCCGGCC GTGGCTGTGG GCCAGTGCCC GCTGGTGGGG
    CCAGGCCCCA TGCACCGCCG CCACCTGCTG CTCCCTGCCA GGGTCCCTAG GCCTGGCCTA
    TCGGAGGCGC TTTCCCTGCT CCTGTTCGCC GTTGTTCTGT CTCGTGCCGC CGCCCTGGGC
    TGCATTGGGT TGGTGGCCCA CGCCGGCCAA CTCACCGCAG TCTGGCGCCG CCCAGGAGCC
    GCCCGCGCTC CCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu20985
    Clone ID Related Accession (Same CDS sequence) NM_001081560.2 , NM_001081560.3
    Accession Version NM_001081560.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1875bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2020-01-03
    Organism Homo sapiens(human)
    Product myotonin-protein kinase isoform 3
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC011530.6, L19268.1, BC062553.1 and AV655848.1. On Jun 2, 2019 this sequence version replaced NM_001081560.2. Transcript Variant: This variant (3) has multiple differences in the presence and absence of exons at its 5' end and in the CDS, compared to variant 1. These differences produce a distinct 5' UTR, cause translation initiation at an alternative start codon, and the loss of an in-frame portion of the coding region, compared to variant 1. The encoded protein (isoform 3, also known as isoform 11) has a distinct N-terminus and is shorter than isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    ATGTCAGCCG AGGTGCGGCT GAGGCGGCTC CAGCAGCTGG TGTTGGACCC GGGCTTCCTG 
    GGGCTGGAGC CCCTGCTCGA CCTTCTCCTG GGCGTCCACC AGGAGCTGGG CGCCTCCGAA
    CTGGCCCAGG ACAAGTACGT GGCCGACTTC TTGCAGTGGG CGGAGCCCAT CGTGGTGAGG
    CTTAAGGAGG TCCGACTGCA GAGGGACGAC TTCGAGATTC TGAAGGTGAT CGGACGCGGG
    GCGTTCAGCG AGGTAGCGGT AGTGAAGATG AAGCAGACGG GCCAGGTGTA TGCCATGAAG
    ATCATGAACA AGTGGGACAT GCTGAAGAGG GGCGAGGTGT CGTGCTTCCG TGAGGAGAGG
    GACGTGTTGG TGAATGGGGA CCGGCGGTGG ATCACGCAGC TGCACTTCGC CTTCCAGGAT
    GAGAACTACC TGTACCTGGT CATGGAGTAT TACGTGGGCG GGGACCTGCT GACACTGCTG
    AGCAAGTTTG GGGAGCGGAT TCCGGCCGAG ATGGCGCGCT TCTACCTGGC GGAGATTGTC
    ATGGCCATAG ACTCGGTGCA CCGGCTTGGC TACGTGCACA GGGACATCAA ACCCGACAAC
    ATCCTGCTGG ACCGCTGTGG CCACATCCGC CTGGCCGACT TCGGCTCTTG CCTCAAGCTG
    CGGGCAGATG GAACGGTGCG GTCGCTGGTG GCTGTGGGCA CCCCAGACTA CCTGTCCCCC
    GAGATCCTGC AGGCTGTGGG CGGTGGGCCT GGGACAGGCA GCTACGGGCC CGAGTGTGAC
    TGGTGGGCGC TGGGTGTATT CGCCTATGAA ATGTTCTATG GGCAGACGCC CTTCTACGCG
    GATTCCACGG CGGAGACCTA TGGCAAGATC GTCCACTACA AGGAGCACCT CTCTCTGCCG
    CTGGTGGACG AAGGGGTCCC TGAGGAGGCT CGAGACTTCA TTCAGCGGTT GCTGTGTCCC
    CCGGAGACAC GGCTGGGCCG GGGTGGAGCA GGCGACTTCC GGACACATCC CTTCTTCTTT
    GGCCTCGACT GGGATGGTCT CCGGGACAGC GTGCCCCCCT TTACACCGGA TTTCGAAGGT
    GCCACCGACA CATGCAACTT CGACTTGGTG GAGGACGGGC TCACTGCCAT GGAGACACTG
    TCGGACATTC GGGAAGGTGC GCCGCTAGGG GTCCACCTGC CTTTTGTGGG CTACTCCTAC
    TCCTGCATGG CCCTCAGGGA CAGTGAGGTC CCAGGCCCCA CACCCATGGA ACTGGAGGCC
    GAGCAGCTGC TTGAGCCACA CGTGCAAGCG CCCAGCCTGG AGCCCTCGGT GTCCCCACAG
    GATGAAACAG CTGAAGTGGC AGTTCCAGCG GCTGTCCCTG CGGCAGAGGC TGAGGCCGAG
    GTGACGCTGC GGGAGCTCCA GGAAGCCCTG GAGGAGGAGG TGCTCACCCG GCAGAGCCTG
    AGCCGGGAGA TGGAGGCCAT CCGCACGGAC AACCAGAACT TCGCCAGTCA ACTACGCGAG
    GCAGAGGCTC GGAACCGGGA CCTAGAGGCA CACGTCCGGC AGTTGCAGGA GCGGATGGAG
    TTGCTGCAGG CAGAGGGAGC CACAGCTGTC ACGGGGGTCC CCAGTCCCCG GGCCACGGAT
    CCACCTTCCC ATCTAGATGG CCCCCCGGCC GTGGCTGTGG GCCAGTGCCC GCTGGTGGGG
    CCAGGCCCCA TGCACCGCCG CCACCTGCTG CTCCCTGCCA GGGTCCCTAG GCCTGGCCTA
    TCGGAGGCGC TTTCCCTGCT CCTGTTCGCC GTTGTTCTGT CTCGTGCCGC CGCCCTGGGC
    TGCATTGGGT TGGTGGCCCA CGCCGGCCAA CTCACCGCAG TCTGGCGCCG CCCAGGAGCC
    GCCCGCGCTC CCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001075029.1
    CDS139..2013
    Translation

    Target ORF information:

    RefSeq Version NM_001081560.3
    Organism Homo sapiens(human)
    Definition Homo sapiens DM1 protein kinase (DMPK), transcript variant 3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001081560.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    ATGTCAGCCG AGGTGCGGCT GAGGCGGCTC CAGCAGCTGG TGTTGGACCC GGGCTTCCTG 
    GGGCTGGAGC CCCTGCTCGA CCTTCTCCTG GGCGTCCACC AGGAGCTGGG CGCCTCCGAA
    CTGGCCCAGG ACAAGTACGT GGCCGACTTC TTGCAGTGGG CGGAGCCCAT CGTGGTGAGG
    CTTAAGGAGG TCCGACTGCA GAGGGACGAC TTCGAGATTC TGAAGGTGAT CGGACGCGGG
    GCGTTCAGCG AGGTAGCGGT AGTGAAGATG AAGCAGACGG GCCAGGTGTA TGCCATGAAG
    ATCATGAACA AGTGGGACAT GCTGAAGAGG GGCGAGGTGT CGTGCTTCCG TGAGGAGAGG
    GACGTGTTGG TGAATGGGGA CCGGCGGTGG ATCACGCAGC TGCACTTCGC CTTCCAGGAT
    GAGAACTACC TGTACCTGGT CATGGAGTAT TACGTGGGCG GGGACCTGCT GACACTGCTG
    AGCAAGTTTG GGGAGCGGAT TCCGGCCGAG ATGGCGCGCT TCTACCTGGC GGAGATTGTC
    ATGGCCATAG ACTCGGTGCA CCGGCTTGGC TACGTGCACA GGGACATCAA ACCCGACAAC
    ATCCTGCTGG ACCGCTGTGG CCACATCCGC CTGGCCGACT TCGGCTCTTG CCTCAAGCTG
    CGGGCAGATG GAACGGTGCG GTCGCTGGTG GCTGTGGGCA CCCCAGACTA CCTGTCCCCC
    GAGATCCTGC AGGCTGTGGG CGGTGGGCCT GGGACAGGCA GCTACGGGCC CGAGTGTGAC
    TGGTGGGCGC TGGGTGTATT CGCCTATGAA ATGTTCTATG GGCAGACGCC CTTCTACGCG
    GATTCCACGG CGGAGACCTA TGGCAAGATC GTCCACTACA AGGAGCACCT CTCTCTGCCG
    CTGGTGGACG AAGGGGTCCC TGAGGAGGCT CGAGACTTCA TTCAGCGGTT GCTGTGTCCC
    CCGGAGACAC GGCTGGGCCG GGGTGGAGCA GGCGACTTCC GGACACATCC CTTCTTCTTT
    GGCCTCGACT GGGATGGTCT CCGGGACAGC GTGCCCCCCT TTACACCGGA TTTCGAAGGT
    GCCACCGACA CATGCAACTT CGACTTGGTG GAGGACGGGC TCACTGCCAT GGAGACACTG
    TCGGACATTC GGGAAGGTGC GCCGCTAGGG GTCCACCTGC CTTTTGTGGG CTACTCCTAC
    TCCTGCATGG CCCTCAGGGA CAGTGAGGTC CCAGGCCCCA CACCCATGGA ACTGGAGGCC
    GAGCAGCTGC TTGAGCCACA CGTGCAAGCG CCCAGCCTGG AGCCCTCGGT GTCCCCACAG
    GATGAAACAG CTGAAGTGGC AGTTCCAGCG GCTGTCCCTG CGGCAGAGGC TGAGGCCGAG
    GTGACGCTGC GGGAGCTCCA GGAAGCCCTG GAGGAGGAGG TGCTCACCCG GCAGAGCCTG
    AGCCGGGAGA TGGAGGCCAT CCGCACGGAC AACCAGAACT TCGCCAGTCA ACTACGCGAG
    GCAGAGGCTC GGAACCGGGA CCTAGAGGCA CACGTCCGGC AGTTGCAGGA GCGGATGGAG
    TTGCTGCAGG CAGAGGGAGC CACAGCTGTC ACGGGGGTCC CCAGTCCCCG GGCCACGGAT
    CCACCTTCCC ATCTAGATGG CCCCCCGGCC GTGGCTGTGG GCCAGTGCCC GCTGGTGGGG
    CCAGGCCCCA TGCACCGCCG CCACCTGCTG CTCCCTGCCA GGGTCCCTAG GCCTGGCCTA
    TCGGAGGCGC TTTCCCTGCT CCTGTTCGCC GTTGTTCTGT CTCGTGCCGC CGCCCTGGGC
    TGCATTGGGT TGGTGGCCCA CGCCGGCCAA CTCACCGCAG TCTGGCGCCG CCCAGGAGCC
    GCCCGCGCTC CCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu21523
    Clone ID Related Accession (Same CDS sequence) NM_001081562.2 , NM_001081562.3
    Accession Version NM_001081562.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1878bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-03-11
    Organism Homo sapiens(human)
    Product myotonin-protein kinase isoform 4
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC011530.6, S72883.1, BC062553.1, AV655848.1 and AC074212.5. On Jan 4, 2014 this sequence version replaced NM_001081562.1. Transcript Variant: This variant (4) has multiple differences in the presence and absence of exons at its 5' end and in the CDS, compared to variant 1. These differences produce a distinct 5' UTR, and cause translation initiation at an alternative start codon, the loss of an in-frame portion of the coding region and a frameshift in the 3' coding region, compared to variant 1. The encoded protein (isoform 4) has a distinct N-terminus and a unique C-terminus, and is shorter than isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: S72883.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    ATGTCAGCCG AGGTGCGGCT GAGGCGGCTC CAGCAGCTGG TGTTGGACCC GGGCTTCCTG 
    GGGCTGGAGC CCCTGCTCGA CCTTCTCCTG GGCGTCCACC AGGAGCTGGG CGCCTCCGAA
    CTGGCCCAGG ACAAGTACGT GGCCGACTTC TTGCAGTGGG CGGAGCCCAT CGTGGTGAGG
    CTTAAGGAGG TCCGACTGCA GAGGGACGAC TTCGAGATTC TGAAGGTGAT CGGACGCGGG
    GCGTTCAGCG AGGTAGCGGT AGTGAAGATG AAGCAGACGG GCCAGGTGTA TGCCATGAAG
    ATCATGAACA AGTGGGACAT GCTGAAGAGG GGCGAGGTGT CGTGCTTCCG TGAGGAGAGG
    GACGTGTTGG TGAATGGGGA CCGGCGGTGG ATCACGCAGC TGCACTTCGC CTTCCAGGAT
    GAGAACTACC TGTACCTGGT CATGGAGTAT TACGTGGGCG GGGACCTGCT GACACTGCTG
    AGCAAGTTTG GGGAGCGGAT TCCGGCCGAG ATGGCGCGCT TCTACCTGGC GGAGATTGTC
    ATGGCCATAG ACTCGGTGCA CCGGCTTGGC TACGTGCACA GGGACATCAA ACCCGACAAC
    ATCCTGCTGG ACCGCTGTGG CCACATCCGC CTGGCCGACT TCGGCTCTTG CCTCAAGCTG
    CGGGCAGATG GAACGGTGCG GTCGCTGGTG GCTGTGGGCA CCCCAGACTA CCTGTCCCCC
    GAGATCCTGC AGGCTGTGGG CGGTGGGCCT GGGACAGGCA GCTACGGGCC CGAGTGTGAC
    TGGTGGGCGC TGGGTGTATT CGCCTATGAA ATGTTCTATG GGCAGACGCC CTTCTACGCG
    GATTCCACGG CGGAGACCTA TGGCAAGATC GTCCACTACA AGGAGCACCT CTCTCTGCCG
    CTGGTGGACG AAGGGGTCCC TGAGGAGGCT CGAGACTTCA TTCAGCGGTT GCTGTGTCCC
    CCGGAGACAC GGCTGGGCCG GGGTGGAGCA GGCGACTTCC GGACACATCC CTTCTTCTTT
    GGCCTCGACT GGGATGGTCT CCGGGACAGC GTGCCCCCCT TTACACCGGA TTTCGAAGGT
    GCCACCGACA CATGCAACTT CGACTTGGTG GAGGACGGGC TCACTGCCAT GGAGACACTG
    TCGGACATTC GGGAAGGTGC GCCGCTAGGG GTCCACCTGC CTTTTGTGGG CTACTCCTAC
    TCCTGCATGG CCCTCAGGGA CAGTGAGGTC CCAGGCCCCA CACCCATGGA ACTGGAGGCC
    GAGCAGCTGC TTGAGCCACA CGTGCAAGCG CCCAGCCTGG AGCCCTCGGT GTCCCCACAG
    GATGAAACAG CTGAAGTGGC AGTTCCAGCG GCTGTCCCTG CGGCAGAGGC TGAGGCCGAG
    GTGACGCTGC GGGAGCTCCA GGAAGCCCTG GAGGAGGAGG TGCTCACCCG GCAGAGCCTG
    AGCCGGGAGA TGGAGGCCAT CCGCACGGAC AACCAGAACT TCGCCAGTCA ACTACGCGAG
    GCAGAGGCTC GGAACCGGGA CCTAGAGGCA CACGTCCGGC AGTTGCAGGA GCGGATGGAG
    TTGCTGCAGG CAGAGGGAGC CACAGCTGTC ACGGGGGTCC CCAGTCCCCG GGCCACGGAT
    CCACCTTCCC ATATGGCCCC CCGGCCGTGG CTGTGGGCCA GTGCCCGCTG GTGGGGCCAG
    GCCCCATGCA CCGCCGCCAC CTGCTGCTCC CTGCCAGGGT CCCTAGGCCT GGCCTATCGG
    AGGCGCTTTC CCTGCTCCTG TTCGCCGTTG TTCTGTCTCG TGCCGCCGCC CTGGGCTGCA
    TTGGGTTGGT GGCCCACGCC GGCCAACTCA CCGCAGTCTG GCGCCGCCCA GGAGCCGCCC
    GCGCTCCCTG AACCCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001075031.1
    CDS206..2083
    Translation

    Target ORF information:

    RefSeq Version NM_001081562.2
    Organism Homo sapiens(human)
    Definition Homo sapiens DM1 protein kinase (DMPK), transcript variant 4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001081562.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    ATGTCAGCCG AGGTGCGGCT GAGGCGGCTC CAGCAGCTGG TGTTGGACCC GGGCTTCCTG 
    GGGCTGGAGC CCCTGCTCGA CCTTCTCCTG GGCGTCCACC AGGAGCTGGG CGCCTCCGAA
    CTGGCCCAGG ACAAGTACGT GGCCGACTTC TTGCAGTGGG CGGAGCCCAT CGTGGTGAGG
    CTTAAGGAGG TCCGACTGCA GAGGGACGAC TTCGAGATTC TGAAGGTGAT CGGACGCGGG
    GCGTTCAGCG AGGTAGCGGT AGTGAAGATG AAGCAGACGG GCCAGGTGTA TGCCATGAAG
    ATCATGAACA AGTGGGACAT GCTGAAGAGG GGCGAGGTGT CGTGCTTCCG TGAGGAGAGG
    GACGTGTTGG TGAATGGGGA CCGGCGGTGG ATCACGCAGC TGCACTTCGC CTTCCAGGAT
    GAGAACTACC TGTACCTGGT CATGGAGTAT TACGTGGGCG GGGACCTGCT GACACTGCTG
    AGCAAGTTTG GGGAGCGGAT TCCGGCCGAG ATGGCGCGCT TCTACCTGGC GGAGATTGTC
    ATGGCCATAG ACTCGGTGCA CCGGCTTGGC TACGTGCACA GGGACATCAA ACCCGACAAC
    ATCCTGCTGG ACCGCTGTGG CCACATCCGC CTGGCCGACT TCGGCTCTTG CCTCAAGCTG
    CGGGCAGATG GAACGGTGCG GTCGCTGGTG GCTGTGGGCA CCCCAGACTA CCTGTCCCCC
    GAGATCCTGC AGGCTGTGGG CGGTGGGCCT GGGACAGGCA GCTACGGGCC CGAGTGTGAC
    TGGTGGGCGC TGGGTGTATT CGCCTATGAA ATGTTCTATG GGCAGACGCC CTTCTACGCG
    GATTCCACGG CGGAGACCTA TGGCAAGATC GTCCACTACA AGGAGCACCT CTCTCTGCCG
    CTGGTGGACG AAGGGGTCCC TGAGGAGGCT CGAGACTTCA TTCAGCGGTT GCTGTGTCCC
    CCGGAGACAC GGCTGGGCCG GGGTGGAGCA GGCGACTTCC GGACACATCC CTTCTTCTTT
    GGCCTCGACT GGGATGGTCT CCGGGACAGC GTGCCCCCCT TTACACCGGA TTTCGAAGGT
    GCCACCGACA CATGCAACTT CGACTTGGTG GAGGACGGGC TCACTGCCAT GGAGACACTG
    TCGGACATTC GGGAAGGTGC GCCGCTAGGG GTCCACCTGC CTTTTGTGGG CTACTCCTAC
    TCCTGCATGG CCCTCAGGGA CAGTGAGGTC CCAGGCCCCA CACCCATGGA ACTGGAGGCC
    GAGCAGCTGC TTGAGCCACA CGTGCAAGCG CCCAGCCTGG AGCCCTCGGT GTCCCCACAG
    GATGAAACAG CTGAAGTGGC AGTTCCAGCG GCTGTCCCTG CGGCAGAGGC TGAGGCCGAG
    GTGACGCTGC GGGAGCTCCA GGAAGCCCTG GAGGAGGAGG TGCTCACCCG GCAGAGCCTG
    AGCCGGGAGA TGGAGGCCAT CCGCACGGAC AACCAGAACT TCGCCAGTCA ACTACGCGAG
    GCAGAGGCTC GGAACCGGGA CCTAGAGGCA CACGTCCGGC AGTTGCAGGA GCGGATGGAG
    TTGCTGCAGG CAGAGGGAGC CACAGCTGTC ACGGGGGTCC CCAGTCCCCG GGCCACGGAT
    CCACCTTCCC ATATGGCCCC CCGGCCGTGG CTGTGGGCCA GTGCCCGCTG GTGGGGCCAG
    GCCCCATGCA CCGCCGCCAC CTGCTGCTCC CTGCCAGGGT CCCTAGGCCT GGCCTATCGG
    AGGCGCTTTC CCTGCTCCTG TTCGCCGTTG TTCTGTCTCG TGCCGCCGCC CTGGGCTGCA
    TTGGGTTGGT GGCCCACGCC GGCCAACTCA CCGCAGTCTG GCGCCGCCCA GGAGCCGCCC
    GCGCTCCCTG AACCCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu21523
    Clone ID Related Accession (Same CDS sequence) NM_001081562.2 , NM_001081562.3
    Accession Version NM_001081562.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1878bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2020-01-03
    Organism Homo sapiens(human)
    Product myotonin-protein kinase isoform 4
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC011530.6, S72883.1, BC062553.1 and AV655848.1. On Jun 2, 2019 this sequence version replaced NM_001081562.2. Transcript Variant: This variant (4) has multiple differences in the presence and absence of exons at its 5' end and in the CDS, compared to variant 1. These differences produce a distinct 5' UTR, and cause translation initiation at an alternative start codon, the loss of an in-frame portion of the coding region and a frameshift in the 3' coding region, compared to variant 1. The encoded protein (isoform 4) has a distinct N-terminus and a unique C-terminus, and is shorter than isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    ATGTCAGCCG AGGTGCGGCT GAGGCGGCTC CAGCAGCTGG TGTTGGACCC GGGCTTCCTG 
    GGGCTGGAGC CCCTGCTCGA CCTTCTCCTG GGCGTCCACC AGGAGCTGGG CGCCTCCGAA
    CTGGCCCAGG ACAAGTACGT GGCCGACTTC TTGCAGTGGG CGGAGCCCAT CGTGGTGAGG
    CTTAAGGAGG TCCGACTGCA GAGGGACGAC TTCGAGATTC TGAAGGTGAT CGGACGCGGG
    GCGTTCAGCG AGGTAGCGGT AGTGAAGATG AAGCAGACGG GCCAGGTGTA TGCCATGAAG
    ATCATGAACA AGTGGGACAT GCTGAAGAGG GGCGAGGTGT CGTGCTTCCG TGAGGAGAGG
    GACGTGTTGG TGAATGGGGA CCGGCGGTGG ATCACGCAGC TGCACTTCGC CTTCCAGGAT
    GAGAACTACC TGTACCTGGT CATGGAGTAT TACGTGGGCG GGGACCTGCT GACACTGCTG
    AGCAAGTTTG GGGAGCGGAT TCCGGCCGAG ATGGCGCGCT TCTACCTGGC GGAGATTGTC
    ATGGCCATAG ACTCGGTGCA CCGGCTTGGC TACGTGCACA GGGACATCAA ACCCGACAAC
    ATCCTGCTGG ACCGCTGTGG CCACATCCGC CTGGCCGACT TCGGCTCTTG CCTCAAGCTG
    CGGGCAGATG GAACGGTGCG GTCGCTGGTG GCTGTGGGCA CCCCAGACTA CCTGTCCCCC
    GAGATCCTGC AGGCTGTGGG CGGTGGGCCT GGGACAGGCA GCTACGGGCC CGAGTGTGAC
    TGGTGGGCGC TGGGTGTATT CGCCTATGAA ATGTTCTATG GGCAGACGCC CTTCTACGCG
    GATTCCACGG CGGAGACCTA TGGCAAGATC GTCCACTACA AGGAGCACCT CTCTCTGCCG
    CTGGTGGACG AAGGGGTCCC TGAGGAGGCT CGAGACTTCA TTCAGCGGTT GCTGTGTCCC
    CCGGAGACAC GGCTGGGCCG GGGTGGAGCA GGCGACTTCC GGACACATCC CTTCTTCTTT
    GGCCTCGACT GGGATGGTCT CCGGGACAGC GTGCCCCCCT TTACACCGGA TTTCGAAGGT
    GCCACCGACA CATGCAACTT CGACTTGGTG GAGGACGGGC TCACTGCCAT GGAGACACTG
    TCGGACATTC GGGAAGGTGC GCCGCTAGGG GTCCACCTGC CTTTTGTGGG CTACTCCTAC
    TCCTGCATGG CCCTCAGGGA CAGTGAGGTC CCAGGCCCCA CACCCATGGA ACTGGAGGCC
    GAGCAGCTGC TTGAGCCACA CGTGCAAGCG CCCAGCCTGG AGCCCTCGGT GTCCCCACAG
    GATGAAACAG CTGAAGTGGC AGTTCCAGCG GCTGTCCCTG CGGCAGAGGC TGAGGCCGAG
    GTGACGCTGC GGGAGCTCCA GGAAGCCCTG GAGGAGGAGG TGCTCACCCG GCAGAGCCTG
    AGCCGGGAGA TGGAGGCCAT CCGCACGGAC AACCAGAACT TCGCCAGTCA ACTACGCGAG
    GCAGAGGCTC GGAACCGGGA CCTAGAGGCA CACGTCCGGC AGTTGCAGGA GCGGATGGAG
    TTGCTGCAGG CAGAGGGAGC CACAGCTGTC ACGGGGGTCC CCAGTCCCCG GGCCACGGAT
    CCACCTTCCC ATATGGCCCC CCGGCCGTGG CTGTGGGCCA GTGCCCGCTG GTGGGGCCAG
    GCCCCATGCA CCGCCGCCAC CTGCTGCTCC CTGCCAGGGT CCCTAGGCCT GGCCTATCGG
    AGGCGCTTTC CCTGCTCCTG TTCGCCGTTG TTCTGTCTCG TGCCGCCGCC CTGGGCTGCA
    TTGGGTTGGT GGCCCACGCC GGCCAACTCA CCGCAGTCTG GCGCCGCCCA GGAGCCGCCC
    GCGCTCCCTG AACCCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001075031.1
    CDS139..2016
    Translation

    Target ORF information:

    RefSeq Version NM_001081562.3
    Organism Homo sapiens(human)
    Definition Homo sapiens DM1 protein kinase (DMPK), transcript variant 4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001081562.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    ATGTCAGCCG AGGTGCGGCT GAGGCGGCTC CAGCAGCTGG TGTTGGACCC GGGCTTCCTG 
    GGGCTGGAGC CCCTGCTCGA CCTTCTCCTG GGCGTCCACC AGGAGCTGGG CGCCTCCGAA
    CTGGCCCAGG ACAAGTACGT GGCCGACTTC TTGCAGTGGG CGGAGCCCAT CGTGGTGAGG
    CTTAAGGAGG TCCGACTGCA GAGGGACGAC TTCGAGATTC TGAAGGTGAT CGGACGCGGG
    GCGTTCAGCG AGGTAGCGGT AGTGAAGATG AAGCAGACGG GCCAGGTGTA TGCCATGAAG
    ATCATGAACA AGTGGGACAT GCTGAAGAGG GGCGAGGTGT CGTGCTTCCG TGAGGAGAGG
    GACGTGTTGG TGAATGGGGA CCGGCGGTGG ATCACGCAGC TGCACTTCGC CTTCCAGGAT
    GAGAACTACC TGTACCTGGT CATGGAGTAT TACGTGGGCG GGGACCTGCT GACACTGCTG
    AGCAAGTTTG GGGAGCGGAT TCCGGCCGAG ATGGCGCGCT TCTACCTGGC GGAGATTGTC
    ATGGCCATAG ACTCGGTGCA CCGGCTTGGC TACGTGCACA GGGACATCAA ACCCGACAAC
    ATCCTGCTGG ACCGCTGTGG CCACATCCGC CTGGCCGACT TCGGCTCTTG CCTCAAGCTG
    CGGGCAGATG GAACGGTGCG GTCGCTGGTG GCTGTGGGCA CCCCAGACTA CCTGTCCCCC
    GAGATCCTGC AGGCTGTGGG CGGTGGGCCT GGGACAGGCA GCTACGGGCC CGAGTGTGAC
    TGGTGGGCGC TGGGTGTATT CGCCTATGAA ATGTTCTATG GGCAGACGCC CTTCTACGCG
    GATTCCACGG CGGAGACCTA TGGCAAGATC GTCCACTACA AGGAGCACCT CTCTCTGCCG
    CTGGTGGACG AAGGGGTCCC TGAGGAGGCT CGAGACTTCA TTCAGCGGTT GCTGTGTCCC
    CCGGAGACAC GGCTGGGCCG GGGTGGAGCA GGCGACTTCC GGACACATCC CTTCTTCTTT
    GGCCTCGACT GGGATGGTCT CCGGGACAGC GTGCCCCCCT TTACACCGGA TTTCGAAGGT
    GCCACCGACA CATGCAACTT CGACTTGGTG GAGGACGGGC TCACTGCCAT GGAGACACTG
    TCGGACATTC GGGAAGGTGC GCCGCTAGGG GTCCACCTGC CTTTTGTGGG CTACTCCTAC
    TCCTGCATGG CCCTCAGGGA CAGTGAGGTC CCAGGCCCCA CACCCATGGA ACTGGAGGCC
    GAGCAGCTGC TTGAGCCACA CGTGCAAGCG CCCAGCCTGG AGCCCTCGGT GTCCCCACAG
    GATGAAACAG CTGAAGTGGC AGTTCCAGCG GCTGTCCCTG CGGCAGAGGC TGAGGCCGAG
    GTGACGCTGC GGGAGCTCCA GGAAGCCCTG GAGGAGGAGG TGCTCACCCG GCAGAGCCTG
    AGCCGGGAGA TGGAGGCCAT CCGCACGGAC AACCAGAACT TCGCCAGTCA ACTACGCGAG
    GCAGAGGCTC GGAACCGGGA CCTAGAGGCA CACGTCCGGC AGTTGCAGGA GCGGATGGAG
    TTGCTGCAGG CAGAGGGAGC CACAGCTGTC ACGGGGGTCC CCAGTCCCCG GGCCACGGAT
    CCACCTTCCC ATATGGCCCC CCGGCCGTGG CTGTGGGCCA GTGCCCGCTG GTGGGGCCAG
    GCCCCATGCA CCGCCGCCAC CTGCTGCTCC CTGCCAGGGT CCCTAGGCCT GGCCTATCGG
    AGGCGCTTTC CCTGCTCCTG TTCGCCGTTG TTCTGTCTCG TGCCGCCGCC CTGGGCTGCA
    TTGGGTTGGT GGCCCACGCC GGCCAACTCA CCGCAGTCTG GCGCCGCCCA GGAGCCGCCC
    GCGCTCCCTG AACCCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu21765
    Clone ID Related Accession (Same CDS sequence) NM_001081563.2
    Accession Version NM_001081563.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1920bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-11-15
    Organism Homo sapiens(human)
    Product myotonin-protein kinase isoform 1
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from M87312.1, AC011530.6, AV655848.1, BC062553.1 and AC074212.5. On Jan 4, 2014 this sequence version replaced NM_001081563.1. Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1), also known as Form I. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: M87312.1, KJ534826.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    ATGGGAGGGC ATTTTTGGCC CCCAGAACCT TACACGGTGT TTATGTGGGG AAGCCCCTGG 
    GAAGCAGACA GTCCTAGGGT GAAGCTGAGA GGCAGAGAGA AGGGGAGACA GACAGAGGGT
    GGGGCTTTCC CCCTTGTCTC CAGTGCCCTT TCTGGTGACC CTCGGTTCTT TTCCCCCACC
    ACCCCCCCAG CGGAGCCCAT CGTGGTGAGG CTTAAGGAGG TCCGACTGCA GAGGGACGAC
    TTCGAGATTC TGAAGGTGAT CGGACGCGGG GCGTTCAGCG AGGTAGCGGT AGTGAAGATG
    AAGCAGACGG GCCAGGTGTA TGCCATGAAG ATCATGAACA AGTGGGACAT GCTGAAGAGG
    GGCGAGGTGT CGTGCTTCCG TGAGGAGAGG GACGTGTTGG TGAATGGGGA CCGGCGGTGG
    ATCACGCAGC TGCACTTCGC CTTCCAGGAT GAGAACTACC TGTACCTGGT CATGGAGTAT
    TACGTGGGCG GGGACCTGCT GACACTGCTG AGCAAGTTTG GGGAGCGGAT TCCGGCCGAG
    ATGGCGCGCT TCTACCTGGC GGAGATTGTC ATGGCCATAG ACTCGGTGCA CCGGCTTGGC
    TACGTGCACA GGGACATCAA ACCCGACAAC ATCCTGCTGG ACCGCTGTGG CCACATCCGC
    CTGGCCGACT TCGGCTCTTG CCTCAAGCTG CGGGCAGATG GAACGGTGCG GTCGCTGGTG
    GCTGTGGGCA CCCCAGACTA CCTGTCCCCC GAGATCCTGC AGGCTGTGGG CGGTGGGCCT
    GGGACAGGCA GCTACGGGCC CGAGTGTGAC TGGTGGGCGC TGGGTGTATT CGCCTATGAA
    ATGTTCTATG GGCAGACGCC CTTCTACGCG GATTCCACGG CGGAGACCTA TGGCAAGATC
    GTCCACTACA AGGAGCACCT CTCTCTGCCG CTGGTGGACG AAGGGGTCCC TGAGGAGGCT
    CGAGACTTCA TTCAGCGGTT GCTGTGTCCC CCGGAGACAC GGCTGGGCCG GGGTGGAGCA
    GGCGACTTCC GGACACATCC CTTCTTCTTT GGCCTCGACT GGGATGGTCT CCGGGACAGC
    GTGCCCCCCT TTACACCGGA TTTCGAAGGT GCCACCGACA CATGCAACTT CGACTTGGTG
    GAGGACGGGC TCACTGCCAT GGTGAGCGGG GGCGGGGAGA CACTGTCGGA CATTCGGGAA
    GGTGCGCCGC TAGGGGTCCA CCTGCCTTTT GTGGGCTACT CCTACTCCTG CATGGCCCTC
    AGGGACAGTG AGGTCCCAGG CCCCACACCC ATGGAACTGG AGGCCGAGCA GCTGCTTGAG
    CCACACGTGC AAGCGCCCAG CCTGGAGCCC TCGGTGTCCC CACAGGATGA AACAGCTGAA
    GTGGCAGTTC CAGCGGCTGT CCCTGCGGCA GAGGCTGAGG CCGAGGTGAC GCTGCGGGAG
    CTCCAGGAAG CCCTGGAGGA GGAGGTGCTC ACCCGGCAGA GCCTGAGCCG GGAGATGGAG
    GCCATCCGCA CGGACAACCA GAACTTCGCC AGTCAACTAC GCGAGGCAGA GGCTCGGAAC
    CGGGACCTAG AGGCACACGT CCGGCAGTTG CAGGAGCGGA TGGAGTTGCT GCAGGCAGAG
    GGAGCCACAG CTGTCACGGG GGTCCCCAGT CCCCGGGCCA CGGATCCACC TTCCCATCTA
    GATGGCCCCC CGGCCGTGGC TGTGGGCCAG TGCCCGCTGG TGGGGCCAGG CCCCATGCAC
    CGCCGCCACC TGCTGCTCCC TGCCAGGGTC CCTAGGCCTG GCCTATCGGA GGCGCTTTCC
    CTGCTCCTGT TCGCCGTTGT TCTGTCTCGT GCCGCCGCCC TGGGCTGCAT TGGGTTGGTG
    GCCCACGCCG GCCAACTCAC CGCAGTCTGG CGCCGCCCAG GAGCCGCCCG CGCTCCCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001075032.1
    CDS545..2464
    Translation

    Target ORF information:

    RefSeq Version NM_001081563.2
    Organism Homo sapiens(human)
    Definition Homo sapiens DM1 protein kinase (DMPK), transcript variant 1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001081563.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    ATGGGAGGGC ATTTTTGGCC CCCAGAACCT TACACGGTGT TTATGTGGGG AAGCCCCTGG 
    GAAGCAGACA GTCCTAGGGT GAAGCTGAGA GGCAGAGAGA AGGGGAGACA GACAGAGGGT
    GGGGCTTTCC CCCTTGTCTC CAGTGCCCTT TCTGGTGACC CTCGGTTCTT TTCCCCCACC
    ACCCCCCCAG CGGAGCCCAT CGTGGTGAGG CTTAAGGAGG TCCGACTGCA GAGGGACGAC
    TTCGAGATTC TGAAGGTGAT CGGACGCGGG GCGTTCAGCG AGGTAGCGGT AGTGAAGATG
    AAGCAGACGG GCCAGGTGTA TGCCATGAAG ATCATGAACA AGTGGGACAT GCTGAAGAGG
    GGCGAGGTGT CGTGCTTCCG TGAGGAGAGG GACGTGTTGG TGAATGGGGA CCGGCGGTGG
    ATCACGCAGC TGCACTTCGC CTTCCAGGAT GAGAACTACC TGTACCTGGT CATGGAGTAT
    TACGTGGGCG GGGACCTGCT GACACTGCTG AGCAAGTTTG GGGAGCGGAT TCCGGCCGAG
    ATGGCGCGCT TCTACCTGGC GGAGATTGTC ATGGCCATAG ACTCGGTGCA CCGGCTTGGC
    TACGTGCACA GGGACATCAA ACCCGACAAC ATCCTGCTGG ACCGCTGTGG CCACATCCGC
    CTGGCCGACT TCGGCTCTTG CCTCAAGCTG CGGGCAGATG GAACGGTGCG GTCGCTGGTG
    GCTGTGGGCA CCCCAGACTA CCTGTCCCCC GAGATCCTGC AGGCTGTGGG CGGTGGGCCT
    GGGACAGGCA GCTACGGGCC CGAGTGTGAC TGGTGGGCGC TGGGTGTATT CGCCTATGAA
    ATGTTCTATG GGCAGACGCC CTTCTACGCG GATTCCACGG CGGAGACCTA TGGCAAGATC
    GTCCACTACA AGGAGCACCT CTCTCTGCCG CTGGTGGACG AAGGGGTCCC TGAGGAGGCT
    CGAGACTTCA TTCAGCGGTT GCTGTGTCCC CCGGAGACAC GGCTGGGCCG GGGTGGAGCA
    GGCGACTTCC GGACACATCC CTTCTTCTTT GGCCTCGACT GGGATGGTCT CCGGGACAGC
    GTGCCCCCCT TTACACCGGA TTTCGAAGGT GCCACCGACA CATGCAACTT CGACTTGGTG
    GAGGACGGGC TCACTGCCAT GGTGAGCGGG GGCGGGGAGA CACTGTCGGA CATTCGGGAA
    GGTGCGCCGC TAGGGGTCCA CCTGCCTTTT GTGGGCTACT CCTACTCCTG CATGGCCCTC
    AGGGACAGTG AGGTCCCAGG CCCCACACCC ATGGAACTGG AGGCCGAGCA GCTGCTTGAG
    CCACACGTGC AAGCGCCCAG CCTGGAGCCC TCGGTGTCCC CACAGGATGA AACAGCTGAA
    GTGGCAGTTC CAGCGGCTGT CCCTGCGGCA GAGGCTGAGG CCGAGGTGAC GCTGCGGGAG
    CTCCAGGAAG CCCTGGAGGA GGAGGTGCTC ACCCGGCAGA GCCTGAGCCG GGAGATGGAG
    GCCATCCGCA CGGACAACCA GAACTTCGCC AGTCAACTAC GCGAGGCAGA GGCTCGGAAC
    CGGGACCTAG AGGCACACGT CCGGCAGTTG CAGGAGCGGA TGGAGTTGCT GCAGGCAGAG
    GGAGCCACAG CTGTCACGGG GGTCCCCAGT CCCCGGGCCA CGGATCCACC TTCCCATCTA
    GATGGCCCCC CGGCCGTGGC TGTGGGCCAG TGCCCGCTGG TGGGGCCAGG CCCCATGCAC
    CGCCGCCACC TGCTGCTCCC TGCCAGGGTC CCTAGGCCTG GCCTATCGGA GGCGCTTTCC
    CTGCTCCTGT TCGCCGTTGT TCTGTCTCGT GCCGCCGCCC TGGGCTGCAT TGGGTTGGTG
    GCCCACGCCG GCCAACTCAC CGCAGTCTGG CGCCGCCCAG GAGCCGCCCG CGCTCCCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu20680
    Clone ID Related Accession (Same CDS sequence) NM_001288766.1 , NM_001288766.2
    Accession Version NM_001288766.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1593bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-03-11
    Organism Homo sapiens(human)
    Product myotonin-protein kinase isoform 7
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC011530.6, HY031977.1, AK302874.1, BC026328.1, BP422066.1, BQ670576.1 and AC074212.5. On Jan 4, 2014 this sequence version replaced XM_005258606.1. Transcript Variant: This variant (7) has multiple differences in the presence and absence of exons at its 5' end, compared to variant 1. These differences produce a distinct 5' UTR, cause translation initiation at an alternative start codon, loss of an in-frame portion of the 3' coding region, and a frameshift in the 3' coding region, compared to variant 1. The encoded protein (isoform 7) has a distinct C-terminus and is shorter than isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK302874.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    ATGTCAGCCG AGGTGCGGCT GAGGCGGCTC CAGCAGCTGG TGTTGGACCC GGGCTTCCTG 
    GGGCTGGAGC CCCTGCTCGA CCTTCTCCTG GGCGTCCACC AGGAGCTGGG CGCCTCCGAA
    CTGGCCCAGG ACAAGTACGT GGCCGACTTC TTGCAGTGGG CGGAGCCCAT CGTGGTGAGG
    CTTAAGGAGG TCCGACTGCA GAGGGACGAC TTCGAGATTC TGAAGGTGAT CGGACGCGGG
    GCGTTCAGCG AGGTAGCGGT AGTGAAGATG AAGCAGACGG GCCAGGTGTA TGCCATGAAG
    ATCATGAACA AGTGGGACAT GCTGAAGAGG GGCGAGGTGT CGTGCTTCCG TGAGGAGAGG
    GACGTGTTGG TGAATGGGGA CCGGCGGTGG ATCACGCAGC TGCACTTCGC CTTCCAGGAT
    GAGAACTACC TGTACCTGGT CATGGAGTAT TACGTGGGCG GGGACCTGCT GACACTGCTG
    AGCAAGTTTG GGGAGCGGAT TCCGGCCGAG ATGGCGCGCT TCTACCTGGC GGAGATTGTC
    ATGGCCATAG ACTCGGTGCA CCGGCTTGGC TACGTGCACA GGGACATCAA ACCCGACAAC
    ATCCTGCTGG ACCGCTGTGG CCACATCCGC CTGGCCGACT TCGGCTCTTG CCTCAAGCTG
    CGGGCAGATG GAACGGTGCG GTCGCTGGTG GCTGTGGGCA CCCCAGACTA CCTGTCCCCC
    GAGATCCTGC AGGCTGTGGG CGGTGGGCCT GGGACAGGCA GCTACGGGCC CGAGTGTGAC
    TGGTGGGCGC TGGGTGTATT CGCCTATGAA ATGTTCTATG GGCAGACGCC CTTCTACGCG
    GATTCCACGG CGGAGACCTA TGGCAAGATC GTCCACTACA AGGAGCACCT CTCTCTGCCG
    CTGGTGGACG AAGGGGTCCC TGAGGAGGCT CGAGACTTCA TTCAGCGGTT GCTGTGTCCC
    CCGGAGACAC GGCTGGGCCG GGGTGGAGCA GGCGACTTCC GGACACATCC CTTCTTCTTT
    GGCCTCGACT GGGATGGTCT CCGGGACAGC GTGCCCCCCT TTACACCGGA TTTCGAAGGT
    GCCACCGACA CATGCAACTT CGACTTGGTG GAGGACGGGC TCACTGCCAT GGAGACACTG
    TCGGACATTC GGGAAGGTGC GCCGCTAGGG GTCCACCTGC CTTTTGTGGG CTACTCCTAC
    TCCTGCATGG CCCTCAGGGA CAGTGAGGTC CCAGGCCCCA CACCCATGGA ACTGGAGGCC
    GAGCAGCTGC TTGAGCCACA CGTGCAAGCG CCCAGCCTGG AGCCCTCGGT GTCCCCACAG
    GATGAAACAG CTGAAGTGGC AGTTCCAGCG GCTGTCCCTG CGGCAGAGGC TGAGGCCGAG
    GTGACGCTGC GGGAGCTCCA GGAAGCCCTG GAGGAGGAGG TGCTCACCCG GCAGAGCCTG
    AGCCGGGAGA TGGAGGCCAT CCGCACGGAC AACCAGAACT TCGCCAGTCA ACTACGCGAG
    GCAGAGGCTC GGAACCGGGA CCTAGAGGCA CACGTCCGGC AGTTGCAGGA GCGGATGGAG
    TTGCTGCAGG CAGAGGGAGC CACAGGTCCC TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001275695.1
    CDS206..1798
    Translation

    Target ORF information:

    RefSeq Version NM_001288766.1
    Organism Homo sapiens(human)
    Definition Homo sapiens DM1 protein kinase (DMPK), transcript variant 7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001288766.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    ATGTCAGCCG AGGTGCGGCT GAGGCGGCTC CAGCAGCTGG TGTTGGACCC GGGCTTCCTG 
    GGGCTGGAGC CCCTGCTCGA CCTTCTCCTG GGCGTCCACC AGGAGCTGGG CGCCTCCGAA
    CTGGCCCAGG ACAAGTACGT GGCCGACTTC TTGCAGTGGG CGGAGCCCAT CGTGGTGAGG
    CTTAAGGAGG TCCGACTGCA GAGGGACGAC TTCGAGATTC TGAAGGTGAT CGGACGCGGG
    GCGTTCAGCG AGGTAGCGGT AGTGAAGATG AAGCAGACGG GCCAGGTGTA TGCCATGAAG
    ATCATGAACA AGTGGGACAT GCTGAAGAGG GGCGAGGTGT CGTGCTTCCG TGAGGAGAGG
    GACGTGTTGG TGAATGGGGA CCGGCGGTGG ATCACGCAGC TGCACTTCGC CTTCCAGGAT
    GAGAACTACC TGTACCTGGT CATGGAGTAT TACGTGGGCG GGGACCTGCT GACACTGCTG
    AGCAAGTTTG GGGAGCGGAT TCCGGCCGAG ATGGCGCGCT TCTACCTGGC GGAGATTGTC
    ATGGCCATAG ACTCGGTGCA CCGGCTTGGC TACGTGCACA GGGACATCAA ACCCGACAAC
    ATCCTGCTGG ACCGCTGTGG CCACATCCGC CTGGCCGACT TCGGCTCTTG CCTCAAGCTG
    CGGGCAGATG GAACGGTGCG GTCGCTGGTG GCTGTGGGCA CCCCAGACTA CCTGTCCCCC
    GAGATCCTGC AGGCTGTGGG CGGTGGGCCT GGGACAGGCA GCTACGGGCC CGAGTGTGAC
    TGGTGGGCGC TGGGTGTATT CGCCTATGAA ATGTTCTATG GGCAGACGCC CTTCTACGCG
    GATTCCACGG CGGAGACCTA TGGCAAGATC GTCCACTACA AGGAGCACCT CTCTCTGCCG
    CTGGTGGACG AAGGGGTCCC TGAGGAGGCT CGAGACTTCA TTCAGCGGTT GCTGTGTCCC
    CCGGAGACAC GGCTGGGCCG GGGTGGAGCA GGCGACTTCC GGACACATCC CTTCTTCTTT
    GGCCTCGACT GGGATGGTCT CCGGGACAGC GTGCCCCCCT TTACACCGGA TTTCGAAGGT
    GCCACCGACA CATGCAACTT CGACTTGGTG GAGGACGGGC TCACTGCCAT GGAGACACTG
    TCGGACATTC GGGAAGGTGC GCCGCTAGGG GTCCACCTGC CTTTTGTGGG CTACTCCTAC
    TCCTGCATGG CCCTCAGGGA CAGTGAGGTC CCAGGCCCCA CACCCATGGA ACTGGAGGCC
    GAGCAGCTGC TTGAGCCACA CGTGCAAGCG CCCAGCCTGG AGCCCTCGGT GTCCCCACAG
    GATGAAACAG CTGAAGTGGC AGTTCCAGCG GCTGTCCCTG CGGCAGAGGC TGAGGCCGAG
    GTGACGCTGC GGGAGCTCCA GGAAGCCCTG GAGGAGGAGG TGCTCACCCG GCAGAGCCTG
    AGCCGGGAGA TGGAGGCCAT CCGCACGGAC AACCAGAACT TCGCCAGTCA ACTACGCGAG
    GCAGAGGCTC GGAACCGGGA CCTAGAGGCA CACGTCCGGC AGTTGCAGGA GCGGATGGAG
    TTGCTGCAGG CAGAGGGAGC CACAGGTCCC TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu20680
    Clone ID Related Accession (Same CDS sequence) NM_001288766.1 , NM_001288766.2
    Accession Version NM_001288766.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1593bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2020-01-03
    Organism Homo sapiens(human)
    Product myotonin-protein kinase isoform 7
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from HY031977.1, AK302874.1, BC026328.1, BP422066.1, BQ670576.1 and AC074212.5. On Jun 2, 2019 this sequence version replaced NM_001288766.1. Transcript Variant: This variant (7) has multiple differences in the presence and absence of exons at its 5' end, compared to variant 1. These differences produce a distinct 5' UTR, cause translation initiation at an alternative start codon, loss of an in-frame portion of the 3' coding region, and a frameshift in the 3' coding region, compared to variant 1. The encoded protein (isoform 7) has a distinct C-terminus and is shorter than isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK302874.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    ATGTCAGCCG AGGTGCGGCT GAGGCGGCTC CAGCAGCTGG TGTTGGACCC GGGCTTCCTG 
    GGGCTGGAGC CCCTGCTCGA CCTTCTCCTG GGCGTCCACC AGGAGCTGGG CGCCTCCGAA
    CTGGCCCAGG ACAAGTACGT GGCCGACTTC TTGCAGTGGG CGGAGCCCAT CGTGGTGAGG
    CTTAAGGAGG TCCGACTGCA GAGGGACGAC TTCGAGATTC TGAAGGTGAT CGGACGCGGG
    GCGTTCAGCG AGGTAGCGGT AGTGAAGATG AAGCAGACGG GCCAGGTGTA TGCCATGAAG
    ATCATGAACA AGTGGGACAT GCTGAAGAGG GGCGAGGTGT CGTGCTTCCG TGAGGAGAGG
    GACGTGTTGG TGAATGGGGA CCGGCGGTGG ATCACGCAGC TGCACTTCGC CTTCCAGGAT
    GAGAACTACC TGTACCTGGT CATGGAGTAT TACGTGGGCG GGGACCTGCT GACACTGCTG
    AGCAAGTTTG GGGAGCGGAT TCCGGCCGAG ATGGCGCGCT TCTACCTGGC GGAGATTGTC
    ATGGCCATAG ACTCGGTGCA CCGGCTTGGC TACGTGCACA GGGACATCAA ACCCGACAAC
    ATCCTGCTGG ACCGCTGTGG CCACATCCGC CTGGCCGACT TCGGCTCTTG CCTCAAGCTG
    CGGGCAGATG GAACGGTGCG GTCGCTGGTG GCTGTGGGCA CCCCAGACTA CCTGTCCCCC
    GAGATCCTGC AGGCTGTGGG CGGTGGGCCT GGGACAGGCA GCTACGGGCC CGAGTGTGAC
    TGGTGGGCGC TGGGTGTATT CGCCTATGAA ATGTTCTATG GGCAGACGCC CTTCTACGCG
    GATTCCACGG CGGAGACCTA TGGCAAGATC GTCCACTACA AGGAGCACCT CTCTCTGCCG
    CTGGTGGACG AAGGGGTCCC TGAGGAGGCT CGAGACTTCA TTCAGCGGTT GCTGTGTCCC
    CCGGAGACAC GGCTGGGCCG GGGTGGAGCA GGCGACTTCC GGACACATCC CTTCTTCTTT
    GGCCTCGACT GGGATGGTCT CCGGGACAGC GTGCCCCCCT TTACACCGGA TTTCGAAGGT
    GCCACCGACA CATGCAACTT CGACTTGGTG GAGGACGGGC TCACTGCCAT GGAGACACTG
    TCGGACATTC GGGAAGGTGC GCCGCTAGGG GTCCACCTGC CTTTTGTGGG CTACTCCTAC
    TCCTGCATGG CCCTCAGGGA CAGTGAGGTC CCAGGCCCCA CACCCATGGA ACTGGAGGCC
    GAGCAGCTGC TTGAGCCACA CGTGCAAGCG CCCAGCCTGG AGCCCTCGGT GTCCCCACAG
    GATGAAACAG CTGAAGTGGC AGTTCCAGCG GCTGTCCCTG CGGCAGAGGC TGAGGCCGAG
    GTGACGCTGC GGGAGCTCCA GGAAGCCCTG GAGGAGGAGG TGCTCACCCG GCAGAGCCTG
    AGCCGGGAGA TGGAGGCCAT CCGCACGGAC AACCAGAACT TCGCCAGTCA ACTACGCGAG
    GCAGAGGCTC GGAACCGGGA CCTAGAGGCA CACGTCCGGC AGTTGCAGGA GCGGATGGAG
    TTGCTGCAGG CAGAGGGAGC CACAGGTCCC TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001275695.1
    CDS139..1731
    Translation

    Target ORF information:

    RefSeq Version NM_001288766.2
    Organism Homo sapiens(human)
    Definition Homo sapiens DM1 protein kinase (DMPK), transcript variant 7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001288766.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    ATGTCAGCCG AGGTGCGGCT GAGGCGGCTC CAGCAGCTGG TGTTGGACCC GGGCTTCCTG 
    GGGCTGGAGC CCCTGCTCGA CCTTCTCCTG GGCGTCCACC AGGAGCTGGG CGCCTCCGAA
    CTGGCCCAGG ACAAGTACGT GGCCGACTTC TTGCAGTGGG CGGAGCCCAT CGTGGTGAGG
    CTTAAGGAGG TCCGACTGCA GAGGGACGAC TTCGAGATTC TGAAGGTGAT CGGACGCGGG
    GCGTTCAGCG AGGTAGCGGT AGTGAAGATG AAGCAGACGG GCCAGGTGTA TGCCATGAAG
    ATCATGAACA AGTGGGACAT GCTGAAGAGG GGCGAGGTGT CGTGCTTCCG TGAGGAGAGG
    GACGTGTTGG TGAATGGGGA CCGGCGGTGG ATCACGCAGC TGCACTTCGC CTTCCAGGAT
    GAGAACTACC TGTACCTGGT CATGGAGTAT TACGTGGGCG GGGACCTGCT GACACTGCTG
    AGCAAGTTTG GGGAGCGGAT TCCGGCCGAG ATGGCGCGCT TCTACCTGGC GGAGATTGTC
    ATGGCCATAG ACTCGGTGCA CCGGCTTGGC TACGTGCACA GGGACATCAA ACCCGACAAC
    ATCCTGCTGG ACCGCTGTGG CCACATCCGC CTGGCCGACT TCGGCTCTTG CCTCAAGCTG
    CGGGCAGATG GAACGGTGCG GTCGCTGGTG GCTGTGGGCA CCCCAGACTA CCTGTCCCCC
    GAGATCCTGC AGGCTGTGGG CGGTGGGCCT GGGACAGGCA GCTACGGGCC CGAGTGTGAC
    TGGTGGGCGC TGGGTGTATT CGCCTATGAA ATGTTCTATG GGCAGACGCC CTTCTACGCG
    GATTCCACGG CGGAGACCTA TGGCAAGATC GTCCACTACA AGGAGCACCT CTCTCTGCCG
    CTGGTGGACG AAGGGGTCCC TGAGGAGGCT CGAGACTTCA TTCAGCGGTT GCTGTGTCCC
    CCGGAGACAC GGCTGGGCCG GGGTGGAGCA GGCGACTTCC GGACACATCC CTTCTTCTTT
    GGCCTCGACT GGGATGGTCT CCGGGACAGC GTGCCCCCCT TTACACCGGA TTTCGAAGGT
    GCCACCGACA CATGCAACTT CGACTTGGTG GAGGACGGGC TCACTGCCAT GGAGACACTG
    TCGGACATTC GGGAAGGTGC GCCGCTAGGG GTCCACCTGC CTTTTGTGGG CTACTCCTAC
    TCCTGCATGG CCCTCAGGGA CAGTGAGGTC CCAGGCCCCA CACCCATGGA ACTGGAGGCC
    GAGCAGCTGC TTGAGCCACA CGTGCAAGCG CCCAGCCTGG AGCCCTCGGT GTCCCCACAG
    GATGAAACAG CTGAAGTGGC AGTTCCAGCG GCTGTCCCTG CGGCAGAGGC TGAGGCCGAG
    GTGACGCTGC GGGAGCTCCA GGAAGCCCTG GAGGAGGAGG TGCTCACCCG GCAGAGCCTG
    AGCCGGGAGA TGGAGGCCAT CCGCACGGAC AACCAGAACT TCGCCAGTCA ACTACGCGAG
    GCAGAGGCTC GGAACCGGGA CCTAGAGGCA CACGTCCGGC AGTTGCAGGA GCGGATGGAG
    TTGCTGCAGG CAGAGGGAGC CACAGGTCCC TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu20965
    Clone ID Related Accession (Same CDS sequence) NM_001288765.1
    Accession Version NM_001288765.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1626bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-11-15
    Organism Homo sapiens(human)
    Product myotonin-protein kinase isoform 6
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC011530.6, DC420602.1, AK297299.1, BC026328.1, M94203.1, BG152972.1 and AC074212.5. Transcript Variant: This variant (6) has multiple differences in the presence and absence of exons at its 5' end, compared to variant 1. These differences produce a distinct 5' UTR, and cause translation initiation at an alternative start codon, compared to variant 1. The encoded protein (isoform 6) has a shorter and distinct N-terminus, compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK297299.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2155984 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    ATGAAGCAGA CGGGCCAGGT GTATGCCATG AAGATCATGA ACAAGTGGGA CATGCTGAAG 
    AGGGGCGAGG TGTCGTGCTT CCGTGAGGAG AGGGACGTGT TGGTGAATGG GGACCGGCGG
    TGGATCACGC AGCTGCACTT CGCCTTCCAG GATGAGAACT ACCTGTACCT GGTCATGGAG
    TATTACGTGG GCGGGGACCT GCTGACACTG CTGAGCAAGT TTGGGGAGCG GATTCCGGCC
    GAGATGGCGC GCTTCTACCT GGCGGAGATT GTCATGGCCA TAGACTCGGT GCACCGGCTT
    GGCTACGTGC ACAGGGACAT CAAACCCGAC AACATCCTGC TGGACCGCTG TGGCCACATC
    CGCCTGGCCG ACTTCGGCTC TTGCCTCAAG CTGCGGGCAG ATGGAACGGT GCGGTCGCTG
    GTGGCTGTGG GCACCCCAGA CTACCTGTCC CCCGAGATCC TGCAGGCTGT GGGCGGTGGG
    CCTGGGACAG GCAGCTACGG GCCCGAGTGT GACTGGTGGG CGCTGGGTGT ATTCGCCTAT
    GAAATGTTCT ATGGGCAGAC GCCCTTCTAC GCGGATTCCA CGGCGGAGAC CTATGGCAAG
    ATCGTCCACT ACAAGGAGCA CCTCTCTCTG CCGCTGGTGG ACGAAGGGGT CCCTGAGGAG
    GCTCGAGACT TCATTCAGCG GTTGCTGTGT CCCCCGGAGA CACGGCTGGG CCGGGGTGGA
    GCAGGCGACT TCCGGACACA TCCCTTCTTC TTTGGCCTCG ACTGGGATGG TCTCCGGGAC
    AGCGTGCCCC CCTTTACACC GGATTTCGAA GGTGCCACCG ACACATGCAA CTTCGACTTG
    GTGGAGGACG GGCTCACTGC CATGGTGAGC GGGGGCGGGG AGACACTGTC GGACATTCGG
    GAAGGTGCGC CGCTAGGGGT CCACCTGCCT TTTGTGGGCT ACTCCTACTC CTGCATGGCC
    CTCAGGGACA GTGAGGTCCC AGGCCCCACA CCCATGGAAC TGGAGGCCGA GCAGCTGCTT
    GAGCCACACG TGCAAGCGCC CAGCCTGGAG CCCTCGGTGT CCCCACAGGA TGAAACAGCT
    GAAGTGGCAG TTCCAGCGGC TGTCCCTGCG GCAGAGGCTG AGGCCGAGGT GACGCTGCGG
    GAGCTCCAGG AAGCCCTGGA GGAGGAGGTG CTCACCCGGC AGAGCCTGAG CCGGGAGATG
    GAGGCCATCC GCACGGACAA CCAGAACTTC GCCAGTCAAC TACGCGAGGC AGAGGCTCGG
    AACCGGGACC TAGAGGCACA CGTCCGGCAG TTGCAGGAGC GGATGGAGTT GCTGCAGGCA
    GAGGGAGCCA CAGCTGTCAC GGGGGTCCCC AGTCCCCGGG CCACGGATCC ACCTTCCCAT
    ATGGCCCCCC GGCCGTGGCT GTGGGCCAGT GCCCGCTGGT GGGGCCAGGC CCCATGCACC
    GCCGCCACCT GCTGCTCCCT GCCAGGGTCC CTAGGCCTGG CCTATCGGAG GCGCTTTCCC
    TGCTCCTGTT CGCCGTTGTT CTGTCTCGTG CCGCCGCCCT GGGCTGCATT GGGTTGGTGG
    CCCACGCCGG CCAACTCACC GCAGTCTGGC GCCGCCCAGG AGCCGCCCGC GCTCCCTGAA
    CCCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001275694.1
    CDS113..1738
    Translation

    Target ORF information:

    RefSeq Version NM_001288765.1
    Organism Homo sapiens(human)
    Definition Homo sapiens DM1 protein kinase (DMPK), transcript variant 6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001288765.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    ATGAAGCAGA CGGGCCAGGT GTATGCCATG AAGATCATGA ACAAGTGGGA CATGCTGAAG 
    AGGGGCGAGG TGTCGTGCTT CCGTGAGGAG AGGGACGTGT TGGTGAATGG GGACCGGCGG
    TGGATCACGC AGCTGCACTT CGCCTTCCAG GATGAGAACT ACCTGTACCT GGTCATGGAG
    TATTACGTGG GCGGGGACCT GCTGACACTG CTGAGCAAGT TTGGGGAGCG GATTCCGGCC
    GAGATGGCGC GCTTCTACCT GGCGGAGATT GTCATGGCCA TAGACTCGGT GCACCGGCTT
    GGCTACGTGC ACAGGGACAT CAAACCCGAC AACATCCTGC TGGACCGCTG TGGCCACATC
    CGCCTGGCCG ACTTCGGCTC TTGCCTCAAG CTGCGGGCAG ATGGAACGGT GCGGTCGCTG
    GTGGCTGTGG GCACCCCAGA CTACCTGTCC CCCGAGATCC TGCAGGCTGT GGGCGGTGGG
    CCTGGGACAG GCAGCTACGG GCCCGAGTGT GACTGGTGGG CGCTGGGTGT ATTCGCCTAT
    GAAATGTTCT ATGGGCAGAC GCCCTTCTAC GCGGATTCCA CGGCGGAGAC CTATGGCAAG
    ATCGTCCACT ACAAGGAGCA CCTCTCTCTG CCGCTGGTGG ACGAAGGGGT CCCTGAGGAG
    GCTCGAGACT TCATTCAGCG GTTGCTGTGT CCCCCGGAGA CACGGCTGGG CCGGGGTGGA
    GCAGGCGACT TCCGGACACA TCCCTTCTTC TTTGGCCTCG ACTGGGATGG TCTCCGGGAC
    AGCGTGCCCC CCTTTACACC GGATTTCGAA GGTGCCACCG ACACATGCAA CTTCGACTTG
    GTGGAGGACG GGCTCACTGC CATGGTGAGC GGGGGCGGGG AGACACTGTC GGACATTCGG
    GAAGGTGCGC CGCTAGGGGT CCACCTGCCT TTTGTGGGCT ACTCCTACTC CTGCATGGCC
    CTCAGGGACA GTGAGGTCCC AGGCCCCACA CCCATGGAAC TGGAGGCCGA GCAGCTGCTT
    GAGCCACACG TGCAAGCGCC CAGCCTGGAG CCCTCGGTGT CCCCACAGGA TGAAACAGCT
    GAAGTGGCAG TTCCAGCGGC TGTCCCTGCG GCAGAGGCTG AGGCCGAGGT GACGCTGCGG
    GAGCTCCAGG AAGCCCTGGA GGAGGAGGTG CTCACCCGGC AGAGCCTGAG CCGGGAGATG
    GAGGCCATCC GCACGGACAA CCAGAACTTC GCCAGTCAAC TACGCGAGGC AGAGGCTCGG
    AACCGGGACC TAGAGGCACA CGTCCGGCAG TTGCAGGAGC GGATGGAGTT GCTGCAGGCA
    GAGGGAGCCA CAGCTGTCAC GGGGGTCCCC AGTCCCCGGG CCACGGATCC ACCTTCCCAT
    ATGGCCCCCC GGCCGTGGCT GTGGGCCAGT GCCCGCTGGT GGGGCCAGGC CCCATGCACC
    GCCGCCACCT GCTGCTCCCT GCCAGGGTCC CTAGGCCTGG CCTATCGGAG GCGCTTTCCC
    TGCTCCTGTT CGCCGTTGTT CTGTCTCGTG CCGCCGCCCT GGGCTGCATT GGGTTGGTGG
    CCCACGCCGG CCAACTCACC GCAGTCTGGC GCCGCCCAGG AGCCGCCCGC GCTCCCTGAA
    CCCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu21375
    Clone ID Related Accession (Same CDS sequence) NM_001288764.2 , NM_001288764.1
    Accession Version NM_001288764.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1968bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-11-19
    Organism Homo sapiens(human)
    Product myotonin-protein kinase isoform 5
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BC026328.1, AC011530.6 and AC074212.5. On Jun 2, 2019 this sequence version replaced NM_001288764.1. Transcript Variant: This variant (5) has multiple differences in the presence and absence of exons at its 5' end, compared to variant 1. These differences produce a distinct 5' UTR, and cause translation initiation at an alternative start codon, compared to variant 1. The encoded protein (isoform 5) has a distinct N-terminus and is longer than isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    ATGGTGCTGC CTGTCCAACA TGTCAGCCGA GGTGCGGCTG AGGCGGCTCC AGCAGCTGGT 
    GTTGGACCCG GGCTTCCTGG GGCTGGAGCC CCTGCTCGAC CTTCTCCTGG GCGTCCACCA
    GGAGCTGGGC GCCTCCGAAC TGGCCCAGGA CAAGTACGTG GCCGACTTCT TGCAGTGGGC
    CCCAAATCCA GGGTTTTCCA AAGTGTGGTT CAAGAACCAC CTGCATCTGA ATCTAGAGCG
    GAGCCCATCG TGGTGAGGCT TAAGGAGGTC CGACTGCAGA GGGACGACTT CGAGATTCTG
    AAGGTGATCG GACGCGGGGC GTTCAGCGAG GTAGCGGTAG TGAAGATGAA GCAGACGGGC
    CAGGTGTATG CCATGAAGAT CATGAACAAG TGGGACATGC TGAAGAGGGG CGAGGTGTCG
    TGCTTCCGTG AGGAGAGGGA CGTGTTGGTG AATGGGGACC GGCGGTGGAT CACGCAGCTG
    CACTTCGCCT TCCAGGATGA GAACTACCTG TACCTGGTCA TGGAGTATTA CGTGGGCGGG
    GACCTGCTGA CACTGCTGAG CAAGTTTGGG GAGCGGATTC CGGCCGAGAT GGCGCGCTTC
    TACCTGGCGG AGATTGTCAT GGCCATAGAC TCGGTGCACC GGCTTGGCTA CGTGCACAGG
    GACATCAAAC CCGACAACAT CCTGCTGGAC CGCTGTGGCC ACATCCGCCT GGCCGACTTC
    GGCTCTTGCC TCAAGCTGCG GGCAGATGGA ACGGTGCGGT CGCTGGTGGC TGTGGGCACC
    CCAGACTACC TGTCCCCCGA GATCCTGCAG GCTGTGGGCG GTGGGCCTGG GACAGGCAGC
    TACGGGCCCG AGTGTGACTG GTGGGCGCTG GGTGTATTCG CCTATGAAAT GTTCTATGGG
    CAGACGCCCT TCTACGCGGA TTCCACGGCG GAGACCTATG GCAAGATCGT CCACTACAAG
    GAGCACCTCT CTCTGCCGCT GGTGGACGAA GGGGTCCCTG AGGAGGCTCG AGACTTCATT
    CAGCGGTTGC TGTGTCCCCC GGAGACACGG CTGGGCCGGG GTGGAGCAGG CGACTTCCGG
    ACACATCCCT TCTTCTTTGG CCTCGACTGG GATGGTCTCC GGGACAGCGT GCCCCCCTTT
    ACACCGGATT TCGAAGGTGC CACCGACACA TGCAACTTCG ACTTGGTGGA GGACGGGCTC
    ACTGCCATGG TGAGCGGGGG CGGGGAGACA CTGTCGGACA TTCGGGAAGG TGCGCCGCTA
    GGGGTCCACC TGCCTTTTGT GGGCTACTCC TACTCCTGCA TGGCCCTCAG GGACAGTGAG
    GTCCCAGGCC CCACACCCAT GGAACTGGAG GCCGAGCAGC TGCTTGAGCC ACACGTGCAA
    GCGCCCAGCC TGGAGCCCTC GGTGTCCCCA CAGGATGAAA CAGCTGAAGT GGCAGTTCCA
    GCGGCTGTCC CTGCGGCAGA GGCTGAGGCC GAGGTGACGC TGCGGGAGCT CCAGGAAGCC
    CTGGAGGAGG AGGTGCTCAC CCGGCAGAGC CTGAGCCGGG AGATGGAGGC CATCCGCACG
    GACAACCAGA ACTTCGCCAG TCAACTACGC GAGGCAGAGG CTCGGAACCG GGACCTAGAG
    GCACACGTCC GGCAGTTGCA GGAGCGGATG GAGTTGCTGC AGGCAGAGGG AGCCACAGCT
    GTCACGGGGG TCCCCAGTCC CCGGGCCACG GATCCACCTT CCCATCTAGA TGGCCCCCCG
    GCCGTGGCTG TGGGCCAGTG CCCGCTGGTG GGGCCAGGCC CCATGCACCG CCGCCACCTG
    CTGCTCCCTG CCAGGGTCCC TAGGCCTGGC CTATCGGAGG CGCTTTCCCT GCTCCTGTTC
    GCCGTTGTTC TGTCTCGTGC CGCCGCCCTG GGCTGCATTG GGTTGGTGGC CCACGCCGGC
    CAACTCACCG CAGTCTGGCG CCGCCCAGGA GCCGCCCGCG CTCCCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001275693.1
    CDS120..2087
    Translation

    Target ORF information:

    RefSeq Version NM_001288764.2
    Organism Homo sapiens(human)
    Definition Homo sapiens DM1 protein kinase (DMPK), transcript variant 5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001288764.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    ATGGTGCTGC CTGTCCAACA TGTCAGCCGA GGTGCGGCTG AGGCGGCTCC AGCAGCTGGT 
    GTTGGACCCG GGCTTCCTGG GGCTGGAGCC CCTGCTCGAC CTTCTCCTGG GCGTCCACCA
    GGAGCTGGGC GCCTCCGAAC TGGCCCAGGA CAAGTACGTG GCCGACTTCT TGCAGTGGGC
    CCCAAATCCA GGGTTTTCCA AAGTGTGGTT CAAGAACCAC CTGCATCTGA ATCTAGAGCG
    GAGCCCATCG TGGTGAGGCT TAAGGAGGTC CGACTGCAGA GGGACGACTT CGAGATTCTG
    AAGGTGATCG GACGCGGGGC GTTCAGCGAG GTAGCGGTAG TGAAGATGAA GCAGACGGGC
    CAGGTGTATG CCATGAAGAT CATGAACAAG TGGGACATGC TGAAGAGGGG CGAGGTGTCG
    TGCTTCCGTG AGGAGAGGGA CGTGTTGGTG AATGGGGACC GGCGGTGGAT CACGCAGCTG
    CACTTCGCCT TCCAGGATGA GAACTACCTG TACCTGGTCA TGGAGTATTA CGTGGGCGGG
    GACCTGCTGA CACTGCTGAG CAAGTTTGGG GAGCGGATTC CGGCCGAGAT GGCGCGCTTC
    TACCTGGCGG AGATTGTCAT GGCCATAGAC TCGGTGCACC GGCTTGGCTA CGTGCACAGG
    GACATCAAAC CCGACAACAT CCTGCTGGAC CGCTGTGGCC ACATCCGCCT GGCCGACTTC
    GGCTCTTGCC TCAAGCTGCG GGCAGATGGA ACGGTGCGGT CGCTGGTGGC TGTGGGCACC
    CCAGACTACC TGTCCCCCGA GATCCTGCAG GCTGTGGGCG GTGGGCCTGG GACAGGCAGC
    TACGGGCCCG AGTGTGACTG GTGGGCGCTG GGTGTATTCG CCTATGAAAT GTTCTATGGG
    CAGACGCCCT TCTACGCGGA TTCCACGGCG GAGACCTATG GCAAGATCGT CCACTACAAG
    GAGCACCTCT CTCTGCCGCT GGTGGACGAA GGGGTCCCTG AGGAGGCTCG AGACTTCATT
    CAGCGGTTGC TGTGTCCCCC GGAGACACGG CTGGGCCGGG GTGGAGCAGG CGACTTCCGG
    ACACATCCCT TCTTCTTTGG CCTCGACTGG GATGGTCTCC GGGACAGCGT GCCCCCCTTT
    ACACCGGATT TCGAAGGTGC CACCGACACA TGCAACTTCG ACTTGGTGGA GGACGGGCTC
    ACTGCCATGG TGAGCGGGGG CGGGGAGACA CTGTCGGACA TTCGGGAAGG TGCGCCGCTA
    GGGGTCCACC TGCCTTTTGT GGGCTACTCC TACTCCTGCA TGGCCCTCAG GGACAGTGAG
    GTCCCAGGCC CCACACCCAT GGAACTGGAG GCCGAGCAGC TGCTTGAGCC ACACGTGCAA
    GCGCCCAGCC TGGAGCCCTC GGTGTCCCCA CAGGATGAAA CAGCTGAAGT GGCAGTTCCA
    GCGGCTGTCC CTGCGGCAGA GGCTGAGGCC GAGGTGACGC TGCGGGAGCT CCAGGAAGCC
    CTGGAGGAGG AGGTGCTCAC CCGGCAGAGC CTGAGCCGGG AGATGGAGGC CATCCGCACG
    GACAACCAGA ACTTCGCCAG TCAACTACGC GAGGCAGAGG CTCGGAACCG GGACCTAGAG
    GCACACGTCC GGCAGTTGCA GGAGCGGATG GAGTTGCTGC AGGCAGAGGG AGCCACAGCT
    GTCACGGGGG TCCCCAGTCC CCGGGCCACG GATCCACCTT CCCATCTAGA TGGCCCCCCG
    GCCGTGGCTG TGGGCCAGTG CCCGCTGGTG GGGCCAGGCC CCATGCACCG CCGCCACCTG
    CTGCTCCCTG CCAGGGTCCC TAGGCCTGGC CTATCGGAGG CGCTTTCCCT GCTCCTGTTC
    GCCGTTGTTC TGTCTCGTGC CGCCGCCCTG GGCTGCATTG GGTTGGTGGC CCACGCCGGC
    CAACTCACCG CAGTCTGGCG CCGCCCAGGA GCCGCCCGCG CTCCCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu21375
    Clone ID Related Accession (Same CDS sequence) NM_001288764.2 , NM_001288764.1
    Accession Version NM_001288764.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1968bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-03-11
    Organism Homo sapiens(human)
    Product myotonin-protein kinase isoform 5
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC011530.6, BG706056.1, BC026328.1 and AC074212.5. On Jan 4, 2014 this sequence version replaced XM_005258605.1. Transcript Variant: This variant (5) has multiple differences in the presence and absence of exons at its 5' end, compared to variant 1. These differences produce a distinct 5' UTR, and cause translation initiation at an alternative start codon, compared to variant 1. The encoded protein (isoform 5) has a distinct N-terminus and is longer than isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC026328.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    ATGGTGCTGC CTGTCCAACA TGTCAGCCGA GGTGCGGCTG AGGCGGCTCC AGCAGCTGGT 
    GTTGGACCCG GGCTTCCTGG GGCTGGAGCC CCTGCTCGAC CTTCTCCTGG GCGTCCACCA
    GGAGCTGGGC GCCTCCGAAC TGGCCCAGGA CAAGTACGTG GCCGACTTCT TGCAGTGGGC
    CCCAAATCCA GGGTTTTCCA AAGTGTGGTT CAAGAACCAC CTGCATCTGA ATCTAGAGCG
    GAGCCCATCG TGGTGAGGCT TAAGGAGGTC CGACTGCAGA GGGACGACTT CGAGATTCTG
    AAGGTGATCG GACGCGGGGC GTTCAGCGAG GTAGCGGTAG TGAAGATGAA GCAGACGGGC
    CAGGTGTATG CCATGAAGAT CATGAACAAG TGGGACATGC TGAAGAGGGG CGAGGTGTCG
    TGCTTCCGTG AGGAGAGGGA CGTGTTGGTG AATGGGGACC GGCGGTGGAT CACGCAGCTG
    CACTTCGCCT TCCAGGATGA GAACTACCTG TACCTGGTCA TGGAGTATTA CGTGGGCGGG
    GACCTGCTGA CACTGCTGAG CAAGTTTGGG GAGCGGATTC CGGCCGAGAT GGCGCGCTTC
    TACCTGGCGG AGATTGTCAT GGCCATAGAC TCGGTGCACC GGCTTGGCTA CGTGCACAGG
    GACATCAAAC CCGACAACAT CCTGCTGGAC CGCTGTGGCC ACATCCGCCT GGCCGACTTC
    GGCTCTTGCC TCAAGCTGCG GGCAGATGGA ACGGTGCGGT CGCTGGTGGC TGTGGGCACC
    CCAGACTACC TGTCCCCCGA GATCCTGCAG GCTGTGGGCG GTGGGCCTGG GACAGGCAGC
    TACGGGCCCG AGTGTGACTG GTGGGCGCTG GGTGTATTCG CCTATGAAAT GTTCTATGGG
    CAGACGCCCT TCTACGCGGA TTCCACGGCG GAGACCTATG GCAAGATCGT CCACTACAAG
    GAGCACCTCT CTCTGCCGCT GGTGGACGAA GGGGTCCCTG AGGAGGCTCG AGACTTCATT
    CAGCGGTTGC TGTGTCCCCC GGAGACACGG CTGGGCCGGG GTGGAGCAGG CGACTTCCGG
    ACACATCCCT TCTTCTTTGG CCTCGACTGG GATGGTCTCC GGGACAGCGT GCCCCCCTTT
    ACACCGGATT TCGAAGGTGC CACCGACACA TGCAACTTCG ACTTGGTGGA GGACGGGCTC
    ACTGCCATGG TGAGCGGGGG CGGGGAGACA CTGTCGGACA TTCGGGAAGG TGCGCCGCTA
    GGGGTCCACC TGCCTTTTGT GGGCTACTCC TACTCCTGCA TGGCCCTCAG GGACAGTGAG
    GTCCCAGGCC CCACACCCAT GGAACTGGAG GCCGAGCAGC TGCTTGAGCC ACACGTGCAA
    GCGCCCAGCC TGGAGCCCTC GGTGTCCCCA CAGGATGAAA CAGCTGAAGT GGCAGTTCCA
    GCGGCTGTCC CTGCGGCAGA GGCTGAGGCC GAGGTGACGC TGCGGGAGCT CCAGGAAGCC
    CTGGAGGAGG AGGTGCTCAC CCGGCAGAGC CTGAGCCGGG AGATGGAGGC CATCCGCACG
    GACAACCAGA ACTTCGCCAG TCAACTACGC GAGGCAGAGG CTCGGAACCG GGACCTAGAG
    GCACACGTCC GGCAGTTGCA GGAGCGGATG GAGTTGCTGC AGGCAGAGGG AGCCACAGCT
    GTCACGGGGG TCCCCAGTCC CCGGGCCACG GATCCACCTT CCCATCTAGA TGGCCCCCCG
    GCCGTGGCTG TGGGCCAGTG CCCGCTGGTG GGGCCAGGCC CCATGCACCG CCGCCACCTG
    CTGCTCCCTG CCAGGGTCCC TAGGCCTGGC CTATCGGAGG CGCTTTCCCT GCTCCTGTTC
    GCCGTTGTTC TGTCTCGTGC CGCCGCCCTG GGCTGCATTG GGTTGGTGGC CCACGCCGGC
    CAACTCACCG CAGTCTGGCG CCGCCCAGGA GCCGCCCGCG CTCCCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001275693.1
    CDS187..2154
    Translation

    Target ORF information:

    RefSeq Version NM_001288764.1
    Organism Homo sapiens(human)
    Definition Homo sapiens DM1 protein kinase (DMPK), transcript variant 5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001288764.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    ATGGTGCTGC CTGTCCAACA TGTCAGCCGA GGTGCGGCTG AGGCGGCTCC AGCAGCTGGT 
    GTTGGACCCG GGCTTCCTGG GGCTGGAGCC CCTGCTCGAC CTTCTCCTGG GCGTCCACCA
    GGAGCTGGGC GCCTCCGAAC TGGCCCAGGA CAAGTACGTG GCCGACTTCT TGCAGTGGGC
    CCCAAATCCA GGGTTTTCCA AAGTGTGGTT CAAGAACCAC CTGCATCTGA ATCTAGAGCG
    GAGCCCATCG TGGTGAGGCT TAAGGAGGTC CGACTGCAGA GGGACGACTT CGAGATTCTG
    AAGGTGATCG GACGCGGGGC GTTCAGCGAG GTAGCGGTAG TGAAGATGAA GCAGACGGGC
    CAGGTGTATG CCATGAAGAT CATGAACAAG TGGGACATGC TGAAGAGGGG CGAGGTGTCG
    TGCTTCCGTG AGGAGAGGGA CGTGTTGGTG AATGGGGACC GGCGGTGGAT CACGCAGCTG
    CACTTCGCCT TCCAGGATGA GAACTACCTG TACCTGGTCA TGGAGTATTA CGTGGGCGGG
    GACCTGCTGA CACTGCTGAG CAAGTTTGGG GAGCGGATTC CGGCCGAGAT GGCGCGCTTC
    TACCTGGCGG AGATTGTCAT GGCCATAGAC TCGGTGCACC GGCTTGGCTA CGTGCACAGG
    GACATCAAAC CCGACAACAT CCTGCTGGAC CGCTGTGGCC ACATCCGCCT GGCCGACTTC
    GGCTCTTGCC TCAAGCTGCG GGCAGATGGA ACGGTGCGGT CGCTGGTGGC TGTGGGCACC
    CCAGACTACC TGTCCCCCGA GATCCTGCAG GCTGTGGGCG GTGGGCCTGG GACAGGCAGC
    TACGGGCCCG AGTGTGACTG GTGGGCGCTG GGTGTATTCG CCTATGAAAT GTTCTATGGG
    CAGACGCCCT TCTACGCGGA TTCCACGGCG GAGACCTATG GCAAGATCGT CCACTACAAG
    GAGCACCTCT CTCTGCCGCT GGTGGACGAA GGGGTCCCTG AGGAGGCTCG AGACTTCATT
    CAGCGGTTGC TGTGTCCCCC GGAGACACGG CTGGGCCGGG GTGGAGCAGG CGACTTCCGG
    ACACATCCCT TCTTCTTTGG CCTCGACTGG GATGGTCTCC GGGACAGCGT GCCCCCCTTT
    ACACCGGATT TCGAAGGTGC CACCGACACA TGCAACTTCG ACTTGGTGGA GGACGGGCTC
    ACTGCCATGG TGAGCGGGGG CGGGGAGACA CTGTCGGACA TTCGGGAAGG TGCGCCGCTA
    GGGGTCCACC TGCCTTTTGT GGGCTACTCC TACTCCTGCA TGGCCCTCAG GGACAGTGAG
    GTCCCAGGCC CCACACCCAT GGAACTGGAG GCCGAGCAGC TGCTTGAGCC ACACGTGCAA
    GCGCCCAGCC TGGAGCCCTC GGTGTCCCCA CAGGATGAAA CAGCTGAAGT GGCAGTTCCA
    GCGGCTGTCC CTGCGGCAGA GGCTGAGGCC GAGGTGACGC TGCGGGAGCT CCAGGAAGCC
    CTGGAGGAGG AGGTGCTCAC CCGGCAGAGC CTGAGCCGGG AGATGGAGGC CATCCGCACG
    GACAACCAGA ACTTCGCCAG TCAACTACGC GAGGCAGAGG CTCGGAACCG GGACCTAGAG
    GCACACGTCC GGCAGTTGCA GGAGCGGATG GAGTTGCTGC AGGCAGAGGG AGCCACAGCT
    GTCACGGGGG TCCCCAGTCC CCGGGCCACG GATCCACCTT CCCATCTAGA TGGCCCCCCG
    GCCGTGGCTG TGGGCCAGTG CCCGCTGGTG GGGCCAGGCC CCATGCACCG CCGCCACCTG
    CTGCTCCCTG CCAGGGTCCC TAGGCCTGGC CTATCGGAGG CGCTTTCCCT GCTCCTGTTC
    GCCGTTGTTC TGTCTCGTGC CGCCGCCCTG GGCTGCATTG GGTTGGTGGC CCACGCCGGC
    CAACTCACCG CAGTCTGGCG CCGCCCAGGA GCCGCCCGCG CTCCCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

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