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TYMP cDNA ORF clone, Homo sapiens(human)

Summary

Gene Symbol TYMP
Entrez Gene ID 1890
Full Name thymidine phosphorylase
Synonyms ECGF,ECGF1,MEDPS1,MNGIE,MTDPS1,PDECGF,TP,hPD-ECGF
General protein information
Preferred Names

thymidine phosphorylase

Names

thymidine phosphorylase
gliostatin
tdRPase
Gene Type protein-coding
Organism Homo sapiens(human)
Genome

22

22q13.33
Summary This gene encodes an angiogenic factor which promotes angiogenesis in vivo and stimulates the in vitro growth of a variety of endothelial cells. It has a highly restricted target cell specificity acting only on endothelial cells. Mutations in this gene have been associated with mitochondrial neurogastrointestinal encephalomyopathy. Multiple alternatively spliced transcript variants have been identified. [provided by RefSeq, Apr 2012].
Disorder MIM:

131222

Disorder Html: Mitochondrial DNA depletion syndrome 1 (MNGIE type), 603041 (3)

Reference Sequences (RefSeq)

mRNA and Protein(s)

mRNA Protein Name
NM_001113756.2 NP_001107228.1 thymidine phosphorylase isoform 1 precursor
NM_001953.4 NP_001944.1 thymidine phosphorylase isoform 1 precursor
NM_001257988.1 NP_001244917.1 thymidine phosphorylase isoform 1 precursor
NM_001257989.1 NP_001244918.1 thymidine phosphorylase isoform 2
NM_001113755.2 NP_001107227.1 thymidine phosphorylase isoform 1 precursor
NM_001113756.3 NP_001107228.1 thymidine phosphorylase isoform 1 precursor
NM_001113755.3 NP_001107227.1 thymidine phosphorylase isoform 1 precursor
NM_001953.5 NP_001944.1 thymidine phosphorylase isoform 1 precursor

Pathways from BioSystems


Homologies


Homo sapiens (human) TYMP NP_001107228.1
Pan troglodytes (chimpanzee) TYMP XP_515224.4
Rattus norvegicus (Norway rat) Tymp NP_001012122.1
Mus musculus (house mouse) Tymp NP_612175.1
Xenopus tropicalis (tropical clawed frog) tymp NP_001120427.1
TYMP XP_001112945.1

Gene Ontology


Bibliography

Related articles in PubMed

Thymidine phosphorylase promotes metastasis and serves as a marker of poor prognosis in hepatocellular carcinoma.
Zhang Q, Zhang Y, Hu X, Qin Y, Zhong W, Meng J, Xiao T, Zhang C, Li M, Chen S, Liu H, Liu Y, Sun T, Yang C
Laboratory investigation; a journal of technical methods and pathology97(8)903-912(2017 Aug)

Poor Prognosis Associated with High Levels of Thymidine Phosphorylase and Thrombocytosis in Patients with Renal Cell Carcinoma.
Huang X, Wang L, Chen Y, Zheng X, Wang X
Urologia internationalis98(2)162-168(2017)

Pinpointing a Factor in Myeloma Bone Disease.

Thymidine phosphorylase exerts complex effects on bone resorption and formation in myeloma.
Liu H, Liu Z, Du J, He J, Lin P, Amini B, Starbuck MW, Novane N, Shah JJ, Davis RE, Hou J, Gagel RF, Yang J
Science translational medicine8(353)353ra113(2016 Aug)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Studied the role of thymidine phosphorylase (TP) in promoting the invasion and metastasis of hepatocellular carcinoma (HCC). It was shown that TP correlated with MMP2 and MMP9 expression, and that TP mediated the migration of tumor cells through activity of MMPs in HCC cell lines.
Title: Thymidine phosphorylase promotes metastasis and serves as a marker of poor prognosis in hepatocellular carcinoma.

TP expression is associated with tumor stage, histological grade and thrombocytosis in patients with renal cell carcinoma
Title: Poor Prognosis Associated with High Levels of Thymidine Phosphorylase and Thrombocytosis in Patients with Renal Cell Carcinoma.

TP(thymidine phosphorylase ) curbed the expression of three proteins-IRF8, RUNX2, and osterix. This downregulation was epigenetically driven: High levels of 2DDR, a product of TP secreted by myeloma cells, activated PI3K/AKT signaling and increased the methyltransferase DNMT3A's expression
Title: Pinpointing a Factor in Myeloma Bone Disease.

Investigated the contribution of myeloma-expressed thymidine phosphorylase (TP) to bone lesions. In osteoblast progenitors, TP up-regulated the methylation of RUNX2 and osterix, leading to decreased bone formation. In osteoclast progenitors, TP up-regulated the methylation of IRF8, leading to increased bone resorption.
Title: Thymidine phosphorylase exerts complex effects on bone resorption and formation in myeloma.

Data show that thymidine phosphorylase (TYMP) positive patients had higher systemic immune-inflammation index (SII) score.
Title: Systemic immune-inflammation index, thymidine phosphorylase and survival of localized gastric cancer patients after curative resection.

The following TYMP gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the TYMP cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OHu22602 NM_001113756.3
Latest version!		 Homo sapiens thymidine phosphorylase (TYMP), transcript variant 3, mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK		 $159.50-$223.30
$319.00
OHu22602 NM_001257988.1
Latest version!		 Homo sapiens thymidine phosphorylase (TYMP), transcript variant 4, mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK		 $159.50-$223.30
$319.00
OHu22602 NM_001953.5
Latest version!		 Homo sapiens thymidine phosphorylase (TYMP), transcript variant 2, mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK		 $159.50-$223.30
$319.00
OHu22602 NM_001113755.3
Latest version!		 Homo sapiens thymidine phosphorylase (TYMP), transcript variant 1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK		 $159.50-$223.30
$319.00
OHu23077 NM_001257989.1
Latest version!		 Homo sapiens thymidine phosphorylase (TYMP), transcript variant 5, mRNA. pcDNA3.1+/C-(K)DYK or customized vector TBD $223.30
$319.00
View Old Accession Versions >>
OHu22602 NM_001113756.2 Homo sapiens thymidine phosphorylase (TYMP), transcript variant 3, mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK		 $159.50-$223.30
$319.00
OHu22602 NM_001113755.2 Homo sapiens thymidine phosphorylase (TYMP), transcript variant 1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK		 $159.50-$223.30
$319.00
OHu22602 NM_001953.4 Homo sapiens thymidine phosphorylase (TYMP), transcript variant 2, mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK		 $159.50-$223.30
$319.00
Hide Old Accession Versions >>

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2-4 Clone 40% OFF
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)


CloneID OHu22602
Clone ID Related Accession (Same CDS sequence) NM_001113756.2 , NM_001953.4 , NM_001257988.1 , NM_001113755.2 , NM_001113756.3 , NM_001113755.3 , NM_001953.5
Accession Version NM_001113756.2 Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 1449bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2019-02-22
Organism Homo sapiens(human)
Product thymidine phosphorylase isoform 1 precursor
Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from U62317.2, M63193.1, BC052211.2 and BM744178.1. On Apr 13, 2012 this sequence version replaced NM_001113756.1. Transcript Variant: This variant (3) differs in the 5' UTR, compared to variant 1. Variants 1, 2, 3 and 4 encode the same isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1163655.300347.1, ERR279847.3916.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1966682, SAMEA1968540 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: inferred from homology ##RefSeq-Attributes-END## 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
ATGGCAGCCT TGATGACCCC GGGAACCGGG GCCCCACCCG CGCCTGGTGA CTTCTCCGGG 
GAAGGGAGCC AGGGACTTCC CGACCCTTCG CCAGAGCCCA AGCAGCTCCC GGAGCTGATC 
CGCATGAAGC GAGACGGAGG CCGCCTGAGC GAAGCGGACA TCAGGGGCTT CGTGGCCGCT 
GTGGTGAATG GGAGCGCGCA GGGCGCACAG ATCGGGGCCA TGCTGATGGC CATCCGACTT 
CGGGGCATGG ATCTGGAGGA GACCTCGGTG CTGACCCAGG CCCTGGCTCA GTCGGGACAG 
CAGCTGGAGT GGCCAGAGGC CTGGCGCCAG CAGCTTGTGG ACAAGCATTC CACAGGGGGT 
GTGGGTGACA AGGTCAGCCT GGTCCTCGCA CCTGCCCTGG CGGCATGTGG CTGCAAGGTG 
CCAATGATCA GCGGACGTGG TCTGGGGCAC ACAGGAGGCA CCTTGGATAA GCTGGAGTCT 
ATTCCTGGAT TCAATGTCAT CCAGAGCCCA GAGCAGATGC AAGTGCTGCT GGACCAGGCG 
GGCTGCTGTA TCGTGGGTCA GAGTGAGCAG CTGGTTCCTG CGGACGGAAT CCTATATGCA 
GCCAGAGATG TGACAGCCAC CGTGGACAGC CTGCCACTCA TCACAGCCTC CATTCTCAGT 
AAGAAACTCG TGGAGGGGCT GTCCGCTCTG GTGGTGGACG TTAAGTTCGG AGGGGCCGCC 
GTCTTCCCCA ACCAGGAGCA GGCCCGGGAG CTGGCAAAGA CGCTGGTTGG CGTGGGAGCC 
AGCCTAGGGC TTCGGGTCGC GGCAGCGCTG ACCGCCATGG ACAAGCCCCT GGGTCGCTGC 
GTGGGCCACG CCCTGGAGGT GGAGGAGGCG CTGCTCTGCA TGGACGGCGC AGGCCCGCCA 
GACTTAAGGG ACCTGGTCAC CACGCTCGGG GGCGCCCTGC TCTGGCTCAG CGGACACGCG 
GGGACTCAGG CCCAGGGCGC TGCCCGGGTG GCCGCGGCGC TGGACGACGG CTCGGCCCTT 
GGCCGCTTCG AGCGGATGCT GGCGGCGCAG GGCGTGGATC CCGGTCTGGC CCGAGCCCTG 
TGCTCGGGAA GTCCCGCAGA ACGCCGGCAG CTGCTGCCTC GCGCCCGGGA GCAGGAGGAG 
CTGCTGGCGC CCGCAGATGG CACCGTGGAG CTGGTCCGGG CGCTGCCGCT GGCGCTGGTG 
CTGCACGAGC TCGGGGCCGG GCGCAGCCGC GCTGGGGAGC CGCTCCGCCT GGGGGTGGGC 
GCAGAGCTGC TGGTCGACGT GGGTCAGAGG CTGCGCCGTG GGACCCCCTG GCTCCGCGTG 
CACCGGGACG GCCCCGCGCT CAGCGGCCCG CAGAGCCGCG CCCTGCAGGA GGCGCTCGTA 
CTCTCCGACC GCGCGCCATT CGCCGCCCCC TCGCCCTTCG CAGAGCTCGT TCTGCCGCCG 
CAGCAATAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001107228.1
CDS377..1825
Translation

Target ORF information:

RefSeq Version NM_001113756.2
Organism Homo sapiens(human)
Definition Homo sapiens thymidine phosphorylase (TYMP), transcript variant 3, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 NM_001113756.2

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
ATGGCAGCCT TGATGACCCC GGGAACCGGG GCCCCACCCG CGCCTGGTGA CTTCTCCGGG 
GAAGGGAGCC AGGGACTTCC CGACCCTTCG CCAGAGCCCA AGCAGCTCCC GGAGCTGATC 
CGCATGAAGC GAGACGGAGG CCGCCTGAGC GAAGCGGACA TCAGGGGCTT CGTGGCCGCT 
GTGGTGAATG GGAGCGCGCA GGGCGCACAG ATCGGGGCCA TGCTGATGGC CATCCGACTT 
CGGGGCATGG ATCTGGAGGA GACCTCGGTG CTGACCCAGG CCCTGGCTCA GTCGGGACAG 
CAGCTGGAGT GGCCAGAGGC CTGGCGCCAG CAGCTTGTGG ACAAGCATTC CACAGGGGGT 
GTGGGTGACA AGGTCAGCCT GGTCCTCGCA CCTGCCCTGG CGGCATGTGG CTGCAAGGTG 
CCAATGATCA GCGGACGTGG TCTGGGGCAC ACAGGAGGCA CCTTGGATAA GCTGGAGTCT 
ATTCCTGGAT TCAATGTCAT CCAGAGCCCA GAGCAGATGC AAGTGCTGCT GGACCAGGCG 
GGCTGCTGTA TCGTGGGTCA GAGTGAGCAG CTGGTTCCTG CGGACGGAAT CCTATATGCA 
GCCAGAGATG TGACAGCCAC CGTGGACAGC CTGCCACTCA TCACAGCCTC CATTCTCAGT 
AAGAAACTCG TGGAGGGGCT GTCCGCTCTG GTGGTGGACG TTAAGTTCGG AGGGGCCGCC 
GTCTTCCCCA ACCAGGAGCA GGCCCGGGAG CTGGCAAAGA CGCTGGTTGG CGTGGGAGCC 
AGCCTAGGGC TTCGGGTCGC GGCAGCGCTG ACCGCCATGG ACAAGCCCCT GGGTCGCTGC 
GTGGGCCACG CCCTGGAGGT GGAGGAGGCG CTGCTCTGCA TGGACGGCGC AGGCCCGCCA 
GACTTAAGGG ACCTGGTCAC CACGCTCGGG GGCGCCCTGC TCTGGCTCAG CGGACACGCG 
GGGACTCAGG CCCAGGGCGC TGCCCGGGTG GCCGCGGCGC TGGACGACGG CTCGGCCCTT 
GGCCGCTTCG AGCGGATGCT GGCGGCGCAG GGCGTGGATC CCGGTCTGGC CCGAGCCCTG 
TGCTCGGGAA GTCCCGCAGA ACGCCGGCAG CTGCTGCCTC GCGCCCGGGA GCAGGAGGAG 
CTGCTGGCGC CCGCAGATGG CACCGTGGAG CTGGTCCGGG CGCTGCCGCT GGCGCTGGTG 
CTGCACGAGC TCGGGGCCGG GCGCAGCCGC GCTGGGGAGC CGCTCCGCCT GGGGGTGGGC 
GCAGAGCTGC TGGTCGACGT GGGTCAGAGG CTGCGCCGTG GGACCCCCTG GCTCCGCGTG 
CACCGGGACG GCCCCGCGCT CAGCGGCCCG CAGAGCCGCG CCCTGCAGGA GGCGCTCGTA 
CTCTCCGACC GCGCGCCATT CGCCGCCCCC TCGCCCTTCG CAGAGCTCGT TCTGCCGCCG 
CAGCAATAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu22602
Clone ID Related Accession (Same CDS sequence) NM_001113756.2 , NM_001953.4 , NM_001257988.1 , NM_001113755.2 , NM_001113756.3 , NM_001113755.3 , NM_001953.5
Accession Version NM_001953.4 Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 1449bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2019-02-22
Organism Homo sapiens(human)
Product thymidine phosphorylase isoform 1 precursor
Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from U62317.2, BC018160.1, BC052211.2 and BM744178.1. On Apr 13, 2012 this sequence version replaced NM_001953.3. Transcript Variant: This variant (2) uses an alternate splice site in the 5' UTR, compared to variant 1. Variants 1, 2, 3 and 4 encode the same isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC018160.1, SRR1163655.527340.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1966682, SAMEA1968540 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: inferred from homology ##RefSeq-Attributes-END## COMPLETENESS: full length. 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
ATGGCAGCCT TGATGACCCC GGGAACCGGG GCCCCACCCG CGCCTGGTGA CTTCTCCGGG 
GAAGGGAGCC AGGGACTTCC CGACCCTTCG CCAGAGCCCA AGCAGCTCCC GGAGCTGATC 
CGCATGAAGC GAGACGGAGG CCGCCTGAGC GAAGCGGACA TCAGGGGCTT CGTGGCCGCT 
GTGGTGAATG GGAGCGCGCA GGGCGCACAG ATCGGGGCCA TGCTGATGGC CATCCGACTT 
CGGGGCATGG ATCTGGAGGA GACCTCGGTG CTGACCCAGG CCCTGGCTCA GTCGGGACAG 
CAGCTGGAGT GGCCAGAGGC CTGGCGCCAG CAGCTTGTGG ACAAGCATTC CACAGGGGGT 
GTGGGTGACA AGGTCAGCCT GGTCCTCGCA CCTGCCCTGG CGGCATGTGG CTGCAAGGTG 
CCAATGATCA GCGGACGTGG TCTGGGGCAC ACAGGAGGCA CCTTGGATAA GCTGGAGTCT 
ATTCCTGGAT TCAATGTCAT CCAGAGCCCA GAGCAGATGC AAGTGCTGCT GGACCAGGCG 
GGCTGCTGTA TCGTGGGTCA GAGTGAGCAG CTGGTTCCTG CGGACGGAAT CCTATATGCA 
GCCAGAGATG TGACAGCCAC CGTGGACAGC CTGCCACTCA TCACAGCCTC CATTCTCAGT 
AAGAAACTCG TGGAGGGGCT GTCCGCTCTG GTGGTGGACG TTAAGTTCGG AGGGGCCGCC 
GTCTTCCCCA ACCAGGAGCA GGCCCGGGAG CTGGCAAAGA CGCTGGTTGG CGTGGGAGCC 
AGCCTAGGGC TTCGGGTCGC GGCAGCGCTG ACCGCCATGG ACAAGCCCCT GGGTCGCTGC 
GTGGGCCACG CCCTGGAGGT GGAGGAGGCG CTGCTCTGCA TGGACGGCGC AGGCCCGCCA 
GACTTAAGGG ACCTGGTCAC CACGCTCGGG GGCGCCCTGC TCTGGCTCAG CGGACACGCG 
GGGACTCAGG CCCAGGGCGC TGCCCGGGTG GCCGCGGCGC TGGACGACGG CTCGGCCCTT 
GGCCGCTTCG AGCGGATGCT GGCGGCGCAG GGCGTGGATC CCGGTCTGGC CCGAGCCCTG 
TGCTCGGGAA GTCCCGCAGA ACGCCGGCAG CTGCTGCCTC GCGCCCGGGA GCAGGAGGAG 
CTGCTGGCGC CCGCAGATGG CACCGTGGAG CTGGTCCGGG CGCTGCCGCT GGCGCTGGTG 
CTGCACGAGC TCGGGGCCGG GCGCAGCCGC GCTGGGGAGC CGCTCCGCCT GGGGGTGGGC 
GCAGAGCTGC TGGTCGACGT GGGTCAGAGG CTGCGCCGTG GGACCCCCTG GCTCCGCGTG 
CACCGGGACG GCCCCGCGCT CAGCGGCCCG CAGAGCCGCG CCCTGCAGGA GGCGCTCGTA 
CTCTCCGACC GCGCGCCATT CGCCGCCCCC TCGCCCTTCG CAGAGCTCGT TCTGCCGCCG 
CAGCAATAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001944.1
CDS193..1641
Translation

Target ORF information:

RefSeq Version NM_001953.4
Organism Homo sapiens(human)
Definition Homo sapiens thymidine phosphorylase (TYMP), transcript variant 2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 NM_001953.4

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
ATGGCAGCCT TGATGACCCC GGGAACCGGG GCCCCACCCG CGCCTGGTGA CTTCTCCGGG 
GAAGGGAGCC AGGGACTTCC CGACCCTTCG CCAGAGCCCA AGCAGCTCCC GGAGCTGATC 
CGCATGAAGC GAGACGGAGG CCGCCTGAGC GAAGCGGACA TCAGGGGCTT CGTGGCCGCT 
GTGGTGAATG GGAGCGCGCA GGGCGCACAG ATCGGGGCCA TGCTGATGGC CATCCGACTT 
CGGGGCATGG ATCTGGAGGA GACCTCGGTG CTGACCCAGG CCCTGGCTCA GTCGGGACAG 
CAGCTGGAGT GGCCAGAGGC CTGGCGCCAG CAGCTTGTGG ACAAGCATTC CACAGGGGGT 
GTGGGTGACA AGGTCAGCCT GGTCCTCGCA CCTGCCCTGG CGGCATGTGG CTGCAAGGTG 
CCAATGATCA GCGGACGTGG TCTGGGGCAC ACAGGAGGCA CCTTGGATAA GCTGGAGTCT 
ATTCCTGGAT TCAATGTCAT CCAGAGCCCA GAGCAGATGC AAGTGCTGCT GGACCAGGCG 
GGCTGCTGTA TCGTGGGTCA GAGTGAGCAG CTGGTTCCTG CGGACGGAAT CCTATATGCA 
GCCAGAGATG TGACAGCCAC CGTGGACAGC CTGCCACTCA TCACAGCCTC CATTCTCAGT 
AAGAAACTCG TGGAGGGGCT GTCCGCTCTG GTGGTGGACG TTAAGTTCGG AGGGGCCGCC 
GTCTTCCCCA ACCAGGAGCA GGCCCGGGAG CTGGCAAAGA CGCTGGTTGG CGTGGGAGCC 
AGCCTAGGGC TTCGGGTCGC GGCAGCGCTG ACCGCCATGG ACAAGCCCCT GGGTCGCTGC 
GTGGGCCACG CCCTGGAGGT GGAGGAGGCG CTGCTCTGCA TGGACGGCGC AGGCCCGCCA 
GACTTAAGGG ACCTGGTCAC CACGCTCGGG GGCGCCCTGC TCTGGCTCAG CGGACACGCG 
GGGACTCAGG CCCAGGGCGC TGCCCGGGTG GCCGCGGCGC TGGACGACGG CTCGGCCCTT 
GGCCGCTTCG AGCGGATGCT GGCGGCGCAG GGCGTGGATC CCGGTCTGGC CCGAGCCCTG 
TGCTCGGGAA GTCCCGCAGA ACGCCGGCAG CTGCTGCCTC GCGCCCGGGA GCAGGAGGAG 
CTGCTGGCGC CCGCAGATGG CACCGTGGAG CTGGTCCGGG CGCTGCCGCT GGCGCTGGTG 
CTGCACGAGC TCGGGGCCGG GCGCAGCCGC GCTGGGGAGC CGCTCCGCCT GGGGGTGGGC 
GCAGAGCTGC TGGTCGACGT GGGTCAGAGG CTGCGCCGTG GGACCCCCTG GCTCCGCGTG 
CACCGGGACG GCCCCGCGCT CAGCGGCCCG CAGAGCCGCG CCCTGCAGGA GGCGCTCGTA 
CTCTCCGACC GCGCGCCATT CGCCGCCCCC TCGCCCTTCG CAGAGCTCGT TCTGCCGCCG 
CAGCAATAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu22602
Clone ID Related Accession (Same CDS sequence) NM_001113756.2 , NM_001953.4 , NM_001257988.1 , NM_001113755.2 , NM_001113756.3 , NM_001113755.3 , NM_001953.5
Accession Version NM_001257988.1 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 1449bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2019-08-12
Organism Homo sapiens(human)
Product thymidine phosphorylase isoform 1 precursor
Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from U62317.2, BC052211.2 and AK225269.1. This sequence is a reference standard in the RefSeqGene project. Transcript Variant: This variant (4) uses an alternate splice site in the 5' UTR, compared to variant 1. Variants 1, 2, 3 and 4 encode the same isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC052211.2, SRR1163658.312218.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968540, SAMEA2153980 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: inferred from homology RefSeq Select criteria :: based on conservation ##RefSeq-Attributes-END## 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
ATGGCAGCCT TGATGACCCC GGGAACCGGG GCCCCACCCG CGCCTGGTGA CTTCTCCGGG 
GAAGGGAGCC AGGGACTTCC CGACCCTTCG CCAGAGCCCA AGCAGCTCCC GGAGCTGATC 
CGCATGAAGC GAGACGGAGG CCGCCTGAGC GAAGCGGACA TCAGGGGCTT CGTGGCCGCT 
GTGGTGAATG GGAGCGCGCA GGGCGCACAG ATCGGGGCCA TGCTGATGGC CATCCGACTT 
CGGGGCATGG ATCTGGAGGA GACCTCGGTG CTGACCCAGG CCCTGGCTCA GTCGGGACAG 
CAGCTGGAGT GGCCAGAGGC CTGGCGCCAG CAGCTTGTGG ACAAGCATTC CACAGGGGGT 
GTGGGTGACA AGGTCAGCCT GGTCCTCGCA CCTGCCCTGG CGGCATGTGG CTGCAAGGTG 
CCAATGATCA GCGGACGTGG TCTGGGGCAC ACAGGAGGCA CCTTGGATAA GCTGGAGTCT 
ATTCCTGGAT TCAATGTCAT CCAGAGCCCA GAGCAGATGC AAGTGCTGCT GGACCAGGCG 
GGCTGCTGTA TCGTGGGTCA GAGTGAGCAG CTGGTTCCTG CGGACGGAAT CCTATATGCA 
GCCAGAGATG TGACAGCCAC CGTGGACAGC CTGCCACTCA TCACAGCCTC CATTCTCAGT 
AAGAAACTCG TGGAGGGGCT GTCCGCTCTG GTGGTGGACG TTAAGTTCGG AGGGGCCGCC 
GTCTTCCCCA ACCAGGAGCA GGCCCGGGAG CTGGCAAAGA CGCTGGTTGG CGTGGGAGCC 
AGCCTAGGGC TTCGGGTCGC GGCAGCGCTG ACCGCCATGG ACAAGCCCCT GGGTCGCTGC 
GTGGGCCACG CCCTGGAGGT GGAGGAGGCG CTGCTCTGCA TGGACGGCGC AGGCCCGCCA 
GACTTAAGGG ACCTGGTCAC CACGCTCGGG GGCGCCCTGC TCTGGCTCAG CGGACACGCG 
GGGACTCAGG CCCAGGGCGC TGCCCGGGTG GCCGCGGCGC TGGACGACGG CTCGGCCCTT 
GGCCGCTTCG AGCGGATGCT GGCGGCGCAG GGCGTGGATC CCGGTCTGGC CCGAGCCCTG 
TGCTCGGGAA GTCCCGCAGA ACGCCGGCAG CTGCTGCCTC GCGCCCGGGA GCAGGAGGAG 
CTGCTGGCGC CCGCAGATGG CACCGTGGAG CTGGTCCGGG CGCTGCCGCT GGCGCTGGTG 
CTGCACGAGC TCGGGGCCGG GCGCAGCCGC GCTGGGGAGC CGCTCCGCCT GGGGGTGGGC 
GCAGAGCTGC TGGTCGACGT GGGTCAGAGG CTGCGCCGTG GGACCCCCTG GCTCCGCGTG 
CACCGGGACG GCCCCGCGCT CAGCGGCCCG CAGAGCCGCG CCCTGCAGGA GGCGCTCGTA 
CTCTCCGACC GCGCGCCATT CGCCGCCCCC TCGCCCTTCG CAGAGCTCGT TCTGCCGCCG 
CAGCAATAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001244917.1
CDS228..1676
Translation

Target ORF information:

RefSeq Version NM_001257988.1
Organism Homo sapiens(human)
Definition Homo sapiens thymidine phosphorylase (TYMP), transcript variant 4, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 NM_001257988.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
ATGGCAGCCT TGATGACCCC GGGAACCGGG GCCCCACCCG CGCCTGGTGA CTTCTCCGGG 
GAAGGGAGCC AGGGACTTCC CGACCCTTCG CCAGAGCCCA AGCAGCTCCC GGAGCTGATC 
CGCATGAAGC GAGACGGAGG CCGCCTGAGC GAAGCGGACA TCAGGGGCTT CGTGGCCGCT 
GTGGTGAATG GGAGCGCGCA GGGCGCACAG ATCGGGGCCA TGCTGATGGC CATCCGACTT 
CGGGGCATGG ATCTGGAGGA GACCTCGGTG CTGACCCAGG CCCTGGCTCA GTCGGGACAG 
CAGCTGGAGT GGCCAGAGGC CTGGCGCCAG CAGCTTGTGG ACAAGCATTC CACAGGGGGT 
GTGGGTGACA AGGTCAGCCT GGTCCTCGCA CCTGCCCTGG CGGCATGTGG CTGCAAGGTG 
CCAATGATCA GCGGACGTGG TCTGGGGCAC ACAGGAGGCA CCTTGGATAA GCTGGAGTCT 
ATTCCTGGAT TCAATGTCAT CCAGAGCCCA GAGCAGATGC AAGTGCTGCT GGACCAGGCG 
GGCTGCTGTA TCGTGGGTCA GAGTGAGCAG CTGGTTCCTG CGGACGGAAT CCTATATGCA 
GCCAGAGATG TGACAGCCAC CGTGGACAGC CTGCCACTCA TCACAGCCTC CATTCTCAGT 
AAGAAACTCG TGGAGGGGCT GTCCGCTCTG GTGGTGGACG TTAAGTTCGG AGGGGCCGCC 
GTCTTCCCCA ACCAGGAGCA GGCCCGGGAG CTGGCAAAGA CGCTGGTTGG CGTGGGAGCC 
AGCCTAGGGC TTCGGGTCGC GGCAGCGCTG ACCGCCATGG ACAAGCCCCT GGGTCGCTGC 
GTGGGCCACG CCCTGGAGGT GGAGGAGGCG CTGCTCTGCA TGGACGGCGC AGGCCCGCCA 
GACTTAAGGG ACCTGGTCAC CACGCTCGGG GGCGCCCTGC TCTGGCTCAG CGGACACGCG 
GGGACTCAGG CCCAGGGCGC TGCCCGGGTG GCCGCGGCGC TGGACGACGG CTCGGCCCTT 
GGCCGCTTCG AGCGGATGCT GGCGGCGCAG GGCGTGGATC CCGGTCTGGC CCGAGCCCTG 
TGCTCGGGAA GTCCCGCAGA ACGCCGGCAG CTGCTGCCTC GCGCCCGGGA GCAGGAGGAG 
CTGCTGGCGC CCGCAGATGG CACCGTGGAG CTGGTCCGGG CGCTGCCGCT GGCGCTGGTG 
CTGCACGAGC TCGGGGCCGG GCGCAGCCGC GCTGGGGAGC CGCTCCGCCT GGGGGTGGGC 
GCAGAGCTGC TGGTCGACGT GGGTCAGAGG CTGCGCCGTG GGACCCCCTG GCTCCGCGTG 
CACCGGGACG GCCCCGCGCT CAGCGGCCCG CAGAGCCGCG CCCTGCAGGA GGCGCTCGTA 
CTCTCCGACC GCGCGCCATT CGCCGCCCCC TCGCCCTTCG CAGAGCTCGT TCTGCCGCCG 
CAGCAATAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu22602
Clone ID Related Accession (Same CDS sequence) NM_001113756.2 , NM_001953.4 , NM_001257988.1 , NM_001113755.2 , NM_001113756.3 , NM_001113755.3 , NM_001953.5
Accession Version NM_001113755.2 Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 1449bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2019-02-22
Organism Homo sapiens(human)
Product thymidine phosphorylase isoform 1 precursor
Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from U62317.2, AK314716.1, BC052211.2 and BM744178.1. On Apr 13, 2012 this sequence version replaced NM_001113755.1. Transcript Variant: This variant (1) and variants 2, 3 and 4 encode the same isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1163655.175764.1, AK314716.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1966682, SAMEA1968540 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: inferred from homology ##RefSeq-Attributes-END## 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
ATGGCAGCCT TGATGACCCC GGGAACCGGG GCCCCACCCG CGCCTGGTGA CTTCTCCGGG 
GAAGGGAGCC AGGGACTTCC CGACCCTTCG CCAGAGCCCA AGCAGCTCCC GGAGCTGATC 
CGCATGAAGC GAGACGGAGG CCGCCTGAGC GAAGCGGACA TCAGGGGCTT CGTGGCCGCT 
GTGGTGAATG GGAGCGCGCA GGGCGCACAG ATCGGGGCCA TGCTGATGGC CATCCGACTT 
CGGGGCATGG ATCTGGAGGA GACCTCGGTG CTGACCCAGG CCCTGGCTCA GTCGGGACAG 
CAGCTGGAGT GGCCAGAGGC CTGGCGCCAG CAGCTTGTGG ACAAGCATTC CACAGGGGGT 
GTGGGTGACA AGGTCAGCCT GGTCCTCGCA CCTGCCCTGG CGGCATGTGG CTGCAAGGTG 
CCAATGATCA GCGGACGTGG TCTGGGGCAC ACAGGAGGCA CCTTGGATAA GCTGGAGTCT 
ATTCCTGGAT TCAATGTCAT CCAGAGCCCA GAGCAGATGC AAGTGCTGCT GGACCAGGCG 
GGCTGCTGTA TCGTGGGTCA GAGTGAGCAG CTGGTTCCTG CGGACGGAAT CCTATATGCA 
GCCAGAGATG TGACAGCCAC CGTGGACAGC CTGCCACTCA TCACAGCCTC CATTCTCAGT 
AAGAAACTCG TGGAGGGGCT GTCCGCTCTG GTGGTGGACG TTAAGTTCGG AGGGGCCGCC 
GTCTTCCCCA ACCAGGAGCA GGCCCGGGAG CTGGCAAAGA CGCTGGTTGG CGTGGGAGCC 
AGCCTAGGGC TTCGGGTCGC GGCAGCGCTG ACCGCCATGG ACAAGCCCCT GGGTCGCTGC 
GTGGGCCACG CCCTGGAGGT GGAGGAGGCG CTGCTCTGCA TGGACGGCGC AGGCCCGCCA 
GACTTAAGGG ACCTGGTCAC CACGCTCGGG GGCGCCCTGC TCTGGCTCAG CGGACACGCG 
GGGACTCAGG CCCAGGGCGC TGCCCGGGTG GCCGCGGCGC TGGACGACGG CTCGGCCCTT 
GGCCGCTTCG AGCGGATGCT GGCGGCGCAG GGCGTGGATC CCGGTCTGGC CCGAGCCCTG 
TGCTCGGGAA GTCCCGCAGA ACGCCGGCAG CTGCTGCCTC GCGCCCGGGA GCAGGAGGAG 
CTGCTGGCGC CCGCAGATGG CACCGTGGAG CTGGTCCGGG CGCTGCCGCT GGCGCTGGTG 
CTGCACGAGC TCGGGGCCGG GCGCAGCCGC GCTGGGGAGC CGCTCCGCCT GGGGGTGGGC 
GCAGAGCTGC TGGTCGACGT GGGTCAGAGG CTGCGCCGTG GGACCCCCTG GCTCCGCGTG 
CACCGGGACG GCCCCGCGCT CAGCGGCCCG CAGAGCCGCG CCCTGCAGGA GGCGCTCGTA 
CTCTCCGACC GCGCGCCATT CGCCGCCCCC TCGCCCTTCG CAGAGCTCGT TCTGCCGCCG 
CAGCAATAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001107227.1
CDS205..1653
Translation

Target ORF information:

RefSeq Version NM_001113755.2
Organism Homo sapiens(human)
Definition Homo sapiens thymidine phosphorylase (TYMP), transcript variant 1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 NM_001113755.2

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
ATGGCAGCCT TGATGACCCC GGGAACCGGG GCCCCACCCG CGCCTGGTGA CTTCTCCGGG 
GAAGGGAGCC AGGGACTTCC CGACCCTTCG CCAGAGCCCA AGCAGCTCCC GGAGCTGATC 
CGCATGAAGC GAGACGGAGG CCGCCTGAGC GAAGCGGACA TCAGGGGCTT CGTGGCCGCT 
GTGGTGAATG GGAGCGCGCA GGGCGCACAG ATCGGGGCCA TGCTGATGGC CATCCGACTT 
CGGGGCATGG ATCTGGAGGA GACCTCGGTG CTGACCCAGG CCCTGGCTCA GTCGGGACAG 
CAGCTGGAGT GGCCAGAGGC CTGGCGCCAG CAGCTTGTGG ACAAGCATTC CACAGGGGGT 
GTGGGTGACA AGGTCAGCCT GGTCCTCGCA CCTGCCCTGG CGGCATGTGG CTGCAAGGTG 
CCAATGATCA GCGGACGTGG TCTGGGGCAC ACAGGAGGCA CCTTGGATAA GCTGGAGTCT 
ATTCCTGGAT TCAATGTCAT CCAGAGCCCA GAGCAGATGC AAGTGCTGCT GGACCAGGCG 
GGCTGCTGTA TCGTGGGTCA GAGTGAGCAG CTGGTTCCTG CGGACGGAAT CCTATATGCA 
GCCAGAGATG TGACAGCCAC CGTGGACAGC CTGCCACTCA TCACAGCCTC CATTCTCAGT 
AAGAAACTCG TGGAGGGGCT GTCCGCTCTG GTGGTGGACG TTAAGTTCGG AGGGGCCGCC 
GTCTTCCCCA ACCAGGAGCA GGCCCGGGAG CTGGCAAAGA CGCTGGTTGG CGTGGGAGCC 
AGCCTAGGGC TTCGGGTCGC GGCAGCGCTG ACCGCCATGG ACAAGCCCCT GGGTCGCTGC 
GTGGGCCACG CCCTGGAGGT GGAGGAGGCG CTGCTCTGCA TGGACGGCGC AGGCCCGCCA 
GACTTAAGGG ACCTGGTCAC CACGCTCGGG GGCGCCCTGC TCTGGCTCAG CGGACACGCG 
GGGACTCAGG CCCAGGGCGC TGCCCGGGTG GCCGCGGCGC TGGACGACGG CTCGGCCCTT 
GGCCGCTTCG AGCGGATGCT GGCGGCGCAG GGCGTGGATC CCGGTCTGGC CCGAGCCCTG 
TGCTCGGGAA GTCCCGCAGA ACGCCGGCAG CTGCTGCCTC GCGCCCGGGA GCAGGAGGAG 
CTGCTGGCGC CCGCAGATGG CACCGTGGAG CTGGTCCGGG CGCTGCCGCT GGCGCTGGTG 
CTGCACGAGC TCGGGGCCGG GCGCAGCCGC GCTGGGGAGC CGCTCCGCCT GGGGGTGGGC 
GCAGAGCTGC TGGTCGACGT GGGTCAGAGG CTGCGCCGTG GGACCCCCTG GCTCCGCGTG 
CACCGGGACG GCCCCGCGCT CAGCGGCCCG CAGAGCCGCG CCCTGCAGGA GGCGCTCGTA 
CTCTCCGACC GCGCGCCATT CGCCGCCCCC TCGCCCTTCG CAGAGCTCGT TCTGCCGCCG 
CAGCAATAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu22602
Clone ID Related Accession (Same CDS sequence) NM_001113756.2 , NM_001953.4 , NM_001257988.1 , NM_001113755.2 , NM_001113756.3 , NM_001113755.3 , NM_001953.5
Accession Version NM_001113756.3 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 1449bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2019-08-12
Organism Homo sapiens(human)
Product thymidine phosphorylase isoform 1 precursor
Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from U62317.2, M63193.1, BC052211.2 and BM744178.1. On Jun 2, 2019 this sequence version replaced NM_001113756.2. Transcript Variant: This variant (3) differs in the 5' UTR, compared to variant 1. Variants 1, 2, 3 and 4 encode the same isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: DRR138517.532115.1, SRR1163655.300347.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1966682, SAMEA1968540 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: inferred from homology ##RefSeq-Attributes-END## 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
ATGGCAGCCT TGATGACCCC GGGAACCGGG GCCCCACCCG CGCCTGGTGA CTTCTCCGGG 
GAAGGGAGCC AGGGACTTCC CGACCCTTCG CCAGAGCCCA AGCAGCTCCC GGAGCTGATC 
CGCATGAAGC GAGACGGAGG CCGCCTGAGC GAAGCGGACA TCAGGGGCTT CGTGGCCGCT 
GTGGTGAATG GGAGCGCGCA GGGCGCACAG ATCGGGGCCA TGCTGATGGC CATCCGACTT 
CGGGGCATGG ATCTGGAGGA GACCTCGGTG CTGACCCAGG CCCTGGCTCA GTCGGGACAG 
CAGCTGGAGT GGCCAGAGGC CTGGCGCCAG CAGCTTGTGG ACAAGCATTC CACAGGGGGT 
GTGGGTGACA AGGTCAGCCT GGTCCTCGCA CCTGCCCTGG CGGCATGTGG CTGCAAGGTG 
CCAATGATCA GCGGACGTGG TCTGGGGCAC ACAGGAGGCA CCTTGGATAA GCTGGAGTCT 
ATTCCTGGAT TCAATGTCAT CCAGAGCCCA GAGCAGATGC AAGTGCTGCT GGACCAGGCG 
GGCTGCTGTA TCGTGGGTCA GAGTGAGCAG CTGGTTCCTG CGGACGGAAT CCTATATGCA 
GCCAGAGATG TGACAGCCAC CGTGGACAGC CTGCCACTCA TCACAGCCTC CATTCTCAGT 
AAGAAACTCG TGGAGGGGCT GTCCGCTCTG GTGGTGGACG TTAAGTTCGG AGGGGCCGCC 
GTCTTCCCCA ACCAGGAGCA GGCCCGGGAG CTGGCAAAGA CGCTGGTTGG CGTGGGAGCC 
AGCCTAGGGC TTCGGGTCGC GGCAGCGCTG ACCGCCATGG ACAAGCCCCT GGGTCGCTGC 
GTGGGCCACG CCCTGGAGGT GGAGGAGGCG CTGCTCTGCA TGGACGGCGC AGGCCCGCCA 
GACTTAAGGG ACCTGGTCAC CACGCTCGGG GGCGCCCTGC TCTGGCTCAG CGGACACGCG 
GGGACTCAGG CCCAGGGCGC TGCCCGGGTG GCCGCGGCGC TGGACGACGG CTCGGCCCTT 
GGCCGCTTCG AGCGGATGCT GGCGGCGCAG GGCGTGGATC CCGGTCTGGC CCGAGCCCTG 
TGCTCGGGAA GTCCCGCAGA ACGCCGGCAG CTGCTGCCTC GCGCCCGGGA GCAGGAGGAG 
CTGCTGGCGC CCGCAGATGG CACCGTGGAG CTGGTCCGGG CGCTGCCGCT GGCGCTGGTG 
CTGCACGAGC TCGGGGCCGG GCGCAGCCGC GCTGGGGAGC CGCTCCGCCT GGGGGTGGGC 
GCAGAGCTGC TGGTCGACGT GGGTCAGAGG CTGCGCCGTG GGACCCCCTG GCTCCGCGTG 
CACCGGGACG GCCCCGCGCT CAGCGGCCCG CAGAGCCGCG CCCTGCAGGA GGCGCTCGTA 
CTCTCCGACC GCGCGCCATT CGCCGCCCCC TCGCCCTTCG CAGAGCTCGT TCTGCCGCCG 
CAGCAATAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001107228.1
CDS304..1752
Translation

Target ORF information:

RefSeq Version NM_001113756.3
Organism Homo sapiens(human)
Definition Homo sapiens thymidine phosphorylase (TYMP), transcript variant 3, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 NM_001113756.3

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
ATGGCAGCCT TGATGACCCC GGGAACCGGG GCCCCACCCG CGCCTGGTGA CTTCTCCGGG 
GAAGGGAGCC AGGGACTTCC CGACCCTTCG CCAGAGCCCA AGCAGCTCCC GGAGCTGATC 
CGCATGAAGC GAGACGGAGG CCGCCTGAGC GAAGCGGACA TCAGGGGCTT CGTGGCCGCT 
GTGGTGAATG GGAGCGCGCA GGGCGCACAG ATCGGGGCCA TGCTGATGGC CATCCGACTT 
CGGGGCATGG ATCTGGAGGA GACCTCGGTG CTGACCCAGG CCCTGGCTCA GTCGGGACAG 
CAGCTGGAGT GGCCAGAGGC CTGGCGCCAG CAGCTTGTGG ACAAGCATTC CACAGGGGGT 
GTGGGTGACA AGGTCAGCCT GGTCCTCGCA CCTGCCCTGG CGGCATGTGG CTGCAAGGTG 
CCAATGATCA GCGGACGTGG TCTGGGGCAC ACAGGAGGCA CCTTGGATAA GCTGGAGTCT 
ATTCCTGGAT TCAATGTCAT CCAGAGCCCA GAGCAGATGC AAGTGCTGCT GGACCAGGCG 
GGCTGCTGTA TCGTGGGTCA GAGTGAGCAG CTGGTTCCTG CGGACGGAAT CCTATATGCA 
GCCAGAGATG TGACAGCCAC CGTGGACAGC CTGCCACTCA TCACAGCCTC CATTCTCAGT 
AAGAAACTCG TGGAGGGGCT GTCCGCTCTG GTGGTGGACG TTAAGTTCGG AGGGGCCGCC 
GTCTTCCCCA ACCAGGAGCA GGCCCGGGAG CTGGCAAAGA CGCTGGTTGG CGTGGGAGCC 
AGCCTAGGGC TTCGGGTCGC GGCAGCGCTG ACCGCCATGG ACAAGCCCCT GGGTCGCTGC 
GTGGGCCACG CCCTGGAGGT GGAGGAGGCG CTGCTCTGCA TGGACGGCGC AGGCCCGCCA 
GACTTAAGGG ACCTGGTCAC CACGCTCGGG GGCGCCCTGC TCTGGCTCAG CGGACACGCG 
GGGACTCAGG CCCAGGGCGC TGCCCGGGTG GCCGCGGCGC TGGACGACGG CTCGGCCCTT 
GGCCGCTTCG AGCGGATGCT GGCGGCGCAG GGCGTGGATC CCGGTCTGGC CCGAGCCCTG 
TGCTCGGGAA GTCCCGCAGA ACGCCGGCAG CTGCTGCCTC GCGCCCGGGA GCAGGAGGAG 
CTGCTGGCGC CCGCAGATGG CACCGTGGAG CTGGTCCGGG CGCTGCCGCT GGCGCTGGTG 
CTGCACGAGC TCGGGGCCGG GCGCAGCCGC GCTGGGGAGC CGCTCCGCCT GGGGGTGGGC 
GCAGAGCTGC TGGTCGACGT GGGTCAGAGG CTGCGCCGTG GGACCCCCTG GCTCCGCGTG 
CACCGGGACG GCCCCGCGCT CAGCGGCCCG CAGAGCCGCG CCCTGCAGGA GGCGCTCGTA 
CTCTCCGACC GCGCGCCATT CGCCGCCCCC TCGCCCTTCG CAGAGCTCGT TCTGCCGCCG 
CAGCAATAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu22602
Clone ID Related Accession (Same CDS sequence) NM_001113756.2 , NM_001953.4 , NM_001257988.1 , NM_001113755.2 , NM_001113756.3 , NM_001113755.3 , NM_001953.5
Accession Version NM_001113755.3 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 1449bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2019-08-12
Organism Homo sapiens(human)
Product thymidine phosphorylase isoform 1 precursor
Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AK314716.1, BC052211.2 and BM744178.1. On Jun 2, 2019 this sequence version replaced NM_001113755.2. Transcript Variant: This variant (1) and variants 2, 3 and 4 encode the same isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK314716.1, SRR1163655.175764.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1966682, SAMEA1968540 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: inferred from homology ##RefSeq-Attributes-END## 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
ATGGCAGCCT TGATGACCCC GGGAACCGGG GCCCCACCCG CGCCTGGTGA CTTCTCCGGG 
GAAGGGAGCC AGGGACTTCC CGACCCTTCG CCAGAGCCCA AGCAGCTCCC GGAGCTGATC 
CGCATGAAGC GAGACGGAGG CCGCCTGAGC GAAGCGGACA TCAGGGGCTT CGTGGCCGCT 
GTGGTGAATG GGAGCGCGCA GGGCGCACAG ATCGGGGCCA TGCTGATGGC CATCCGACTT 
CGGGGCATGG ATCTGGAGGA GACCTCGGTG CTGACCCAGG CCCTGGCTCA GTCGGGACAG 
CAGCTGGAGT GGCCAGAGGC CTGGCGCCAG CAGCTTGTGG ACAAGCATTC CACAGGGGGT 
GTGGGTGACA AGGTCAGCCT GGTCCTCGCA CCTGCCCTGG CGGCATGTGG CTGCAAGGTG 
CCAATGATCA GCGGACGTGG TCTGGGGCAC ACAGGAGGCA CCTTGGATAA GCTGGAGTCT 
ATTCCTGGAT TCAATGTCAT CCAGAGCCCA GAGCAGATGC AAGTGCTGCT GGACCAGGCG 
GGCTGCTGTA TCGTGGGTCA GAGTGAGCAG CTGGTTCCTG CGGACGGAAT CCTATATGCA 
GCCAGAGATG TGACAGCCAC CGTGGACAGC CTGCCACTCA TCACAGCCTC CATTCTCAGT 
AAGAAACTCG TGGAGGGGCT GTCCGCTCTG GTGGTGGACG TTAAGTTCGG AGGGGCCGCC 
GTCTTCCCCA ACCAGGAGCA GGCCCGGGAG CTGGCAAAGA CGCTGGTTGG CGTGGGAGCC 
AGCCTAGGGC TTCGGGTCGC GGCAGCGCTG ACCGCCATGG ACAAGCCCCT GGGTCGCTGC 
GTGGGCCACG CCCTGGAGGT GGAGGAGGCG CTGCTCTGCA TGGACGGCGC AGGCCCGCCA 
GACTTAAGGG ACCTGGTCAC CACGCTCGGG GGCGCCCTGC TCTGGCTCAG CGGACACGCG 
GGGACTCAGG CCCAGGGCGC TGCCCGGGTG GCCGCGGCGC TGGACGACGG CTCGGCCCTT 
GGCCGCTTCG AGCGGATGCT GGCGGCGCAG GGCGTGGATC CCGGTCTGGC CCGAGCCCTG 
TGCTCGGGAA GTCCCGCAGA ACGCCGGCAG CTGCTGCCTC GCGCCCGGGA GCAGGAGGAG 
CTGCTGGCGC CCGCAGATGG CACCGTGGAG CTGGTCCGGG CGCTGCCGCT GGCGCTGGTG 
CTGCACGAGC TCGGGGCCGG GCGCAGCCGC GCTGGGGAGC CGCTCCGCCT GGGGGTGGGC 
GCAGAGCTGC TGGTCGACGT GGGTCAGAGG CTGCGCCGTG GGACCCCCTG GCTCCGCGTG 
CACCGGGACG GCCCCGCGCT CAGCGGCCCG CAGAGCCGCG CCCTGCAGGA GGCGCTCGTA 
CTCTCCGACC GCGCGCCATT CGCCGCCCCC TCGCCCTTCG CAGAGCTCGT TCTGCCGCCG 
CAGCAATAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001107227.1
CDS132..1580
Translation

Target ORF information:

RefSeq Version NM_001113755.3
Organism Homo sapiens(human)
Definition Homo sapiens thymidine phosphorylase (TYMP), transcript variant 1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 NM_001113755.3

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
ATGGCAGCCT TGATGACCCC GGGAACCGGG GCCCCACCCG CGCCTGGTGA CTTCTCCGGG 
GAAGGGAGCC AGGGACTTCC CGACCCTTCG CCAGAGCCCA AGCAGCTCCC GGAGCTGATC 
CGCATGAAGC GAGACGGAGG CCGCCTGAGC GAAGCGGACA TCAGGGGCTT CGTGGCCGCT 
GTGGTGAATG GGAGCGCGCA GGGCGCACAG ATCGGGGCCA TGCTGATGGC CATCCGACTT 
CGGGGCATGG ATCTGGAGGA GACCTCGGTG CTGACCCAGG CCCTGGCTCA GTCGGGACAG 
CAGCTGGAGT GGCCAGAGGC CTGGCGCCAG CAGCTTGTGG ACAAGCATTC CACAGGGGGT 
GTGGGTGACA AGGTCAGCCT GGTCCTCGCA CCTGCCCTGG CGGCATGTGG CTGCAAGGTG 
CCAATGATCA GCGGACGTGG TCTGGGGCAC ACAGGAGGCA CCTTGGATAA GCTGGAGTCT 
ATTCCTGGAT TCAATGTCAT CCAGAGCCCA GAGCAGATGC AAGTGCTGCT GGACCAGGCG 
GGCTGCTGTA TCGTGGGTCA GAGTGAGCAG CTGGTTCCTG CGGACGGAAT CCTATATGCA 
GCCAGAGATG TGACAGCCAC CGTGGACAGC CTGCCACTCA TCACAGCCTC CATTCTCAGT 
AAGAAACTCG TGGAGGGGCT GTCCGCTCTG GTGGTGGACG TTAAGTTCGG AGGGGCCGCC 
GTCTTCCCCA ACCAGGAGCA GGCCCGGGAG CTGGCAAAGA CGCTGGTTGG CGTGGGAGCC 
AGCCTAGGGC TTCGGGTCGC GGCAGCGCTG ACCGCCATGG ACAAGCCCCT GGGTCGCTGC 
GTGGGCCACG CCCTGGAGGT GGAGGAGGCG CTGCTCTGCA TGGACGGCGC AGGCCCGCCA 
GACTTAAGGG ACCTGGTCAC CACGCTCGGG GGCGCCCTGC TCTGGCTCAG CGGACACGCG 
GGGACTCAGG CCCAGGGCGC TGCCCGGGTG GCCGCGGCGC TGGACGACGG CTCGGCCCTT 
GGCCGCTTCG AGCGGATGCT GGCGGCGCAG GGCGTGGATC CCGGTCTGGC CCGAGCCCTG 
TGCTCGGGAA GTCCCGCAGA ACGCCGGCAG CTGCTGCCTC GCGCCCGGGA GCAGGAGGAG 
CTGCTGGCGC CCGCAGATGG CACCGTGGAG CTGGTCCGGG CGCTGCCGCT GGCGCTGGTG 
CTGCACGAGC TCGGGGCCGG GCGCAGCCGC GCTGGGGAGC CGCTCCGCCT GGGGGTGGGC 
GCAGAGCTGC TGGTCGACGT GGGTCAGAGG CTGCGCCGTG GGACCCCCTG GCTCCGCGTG 
CACCGGGACG GCCCCGCGCT CAGCGGCCCG CAGAGCCGCG CCCTGCAGGA GGCGCTCGTA 
CTCTCCGACC GCGCGCCATT CGCCGCCCCC TCGCCCTTCG CAGAGCTCGT TCTGCCGCCG 
CAGCAATAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu22602
Clone ID Related Accession (Same CDS sequence) NM_001113756.2 , NM_001953.4 , NM_001257988.1 , NM_001113755.2 , NM_001113756.3 , NM_001113755.3 , NM_001953.5
Accession Version NM_001953.5 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 1449bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2019-08-12
Organism Homo sapiens(human)
Product thymidine phosphorylase isoform 1 precursor
Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from U62317.2, BC018160.1, BC052211.2 and BM744178.1. On Jun 2, 2019 this sequence version replaced NM_001953.4. Transcript Variant: This variant (2) uses an alternate splice site in the 5' UTR, compared to variant 1. Variants 1, 2, 3 and 4 encode the same isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC018160.1, SRR7346977.97705.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1966682, SAMEA1968540 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: inferred from homology ##RefSeq-Attributes-END## COMPLETENESS: full length. 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
ATGGCAGCCT TGATGACCCC GGGAACCGGG GCCCCACCCG CGCCTGGTGA CTTCTCCGGG 
GAAGGGAGCC AGGGACTTCC CGACCCTTCG CCAGAGCCCA AGCAGCTCCC GGAGCTGATC 
CGCATGAAGC GAGACGGAGG CCGCCTGAGC GAAGCGGACA TCAGGGGCTT CGTGGCCGCT 
GTGGTGAATG GGAGCGCGCA GGGCGCACAG ATCGGGGCCA TGCTGATGGC CATCCGACTT 
CGGGGCATGG ATCTGGAGGA GACCTCGGTG CTGACCCAGG CCCTGGCTCA GTCGGGACAG 
CAGCTGGAGT GGCCAGAGGC CTGGCGCCAG CAGCTTGTGG ACAAGCATTC CACAGGGGGT 
GTGGGTGACA AGGTCAGCCT GGTCCTCGCA CCTGCCCTGG CGGCATGTGG CTGCAAGGTG 
CCAATGATCA GCGGACGTGG TCTGGGGCAC ACAGGAGGCA CCTTGGATAA GCTGGAGTCT 
ATTCCTGGAT TCAATGTCAT CCAGAGCCCA GAGCAGATGC AAGTGCTGCT GGACCAGGCG 
GGCTGCTGTA TCGTGGGTCA GAGTGAGCAG CTGGTTCCTG CGGACGGAAT CCTATATGCA 
GCCAGAGATG TGACAGCCAC CGTGGACAGC CTGCCACTCA TCACAGCCTC CATTCTCAGT 
AAGAAACTCG TGGAGGGGCT GTCCGCTCTG GTGGTGGACG TTAAGTTCGG AGGGGCCGCC 
GTCTTCCCCA ACCAGGAGCA GGCCCGGGAG CTGGCAAAGA CGCTGGTTGG CGTGGGAGCC 
AGCCTAGGGC TTCGGGTCGC GGCAGCGCTG ACCGCCATGG ACAAGCCCCT GGGTCGCTGC 
GTGGGCCACG CCCTGGAGGT GGAGGAGGCG CTGCTCTGCA TGGACGGCGC AGGCCCGCCA 
GACTTAAGGG ACCTGGTCAC CACGCTCGGG GGCGCCCTGC TCTGGCTCAG CGGACACGCG 
GGGACTCAGG CCCAGGGCGC TGCCCGGGTG GCCGCGGCGC TGGACGACGG CTCGGCCCTT 
GGCCGCTTCG AGCGGATGCT GGCGGCGCAG GGCGTGGATC CCGGTCTGGC CCGAGCCCTG 
TGCTCGGGAA GTCCCGCAGA ACGCCGGCAG CTGCTGCCTC GCGCCCGGGA GCAGGAGGAG 
CTGCTGGCGC CCGCAGATGG CACCGTGGAG CTGGTCCGGG CGCTGCCGCT GGCGCTGGTG 
CTGCACGAGC TCGGGGCCGG GCGCAGCCGC GCTGGGGAGC CGCTCCGCCT GGGGGTGGGC 
GCAGAGCTGC TGGTCGACGT GGGTCAGAGG CTGCGCCGTG GGACCCCCTG GCTCCGCGTG 
CACCGGGACG GCCCCGCGCT CAGCGGCCCG CAGAGCCGCG CCCTGCAGGA GGCGCTCGTA 
CTCTCCGACC GCGCGCCATT CGCCGCCCCC TCGCCCTTCG CAGAGCTCGT TCTGCCGCCG 
CAGCAATAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001944.1
CDS120..1568
Translation

Target ORF information:

RefSeq Version NM_001953.5
Organism Homo sapiens(human)
Definition Homo sapiens thymidine phosphorylase (TYMP), transcript variant 2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 NM_001953.5

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
ATGGCAGCCT TGATGACCCC GGGAACCGGG GCCCCACCCG CGCCTGGTGA CTTCTCCGGG 
GAAGGGAGCC AGGGACTTCC CGACCCTTCG CCAGAGCCCA AGCAGCTCCC GGAGCTGATC 
CGCATGAAGC GAGACGGAGG CCGCCTGAGC GAAGCGGACA TCAGGGGCTT CGTGGCCGCT 
GTGGTGAATG GGAGCGCGCA GGGCGCACAG ATCGGGGCCA TGCTGATGGC CATCCGACTT 
CGGGGCATGG ATCTGGAGGA GACCTCGGTG CTGACCCAGG CCCTGGCTCA GTCGGGACAG 
CAGCTGGAGT GGCCAGAGGC CTGGCGCCAG CAGCTTGTGG ACAAGCATTC CACAGGGGGT 
GTGGGTGACA AGGTCAGCCT GGTCCTCGCA CCTGCCCTGG CGGCATGTGG CTGCAAGGTG 
CCAATGATCA GCGGACGTGG TCTGGGGCAC ACAGGAGGCA CCTTGGATAA GCTGGAGTCT 
ATTCCTGGAT TCAATGTCAT CCAGAGCCCA GAGCAGATGC AAGTGCTGCT GGACCAGGCG 
GGCTGCTGTA TCGTGGGTCA GAGTGAGCAG CTGGTTCCTG CGGACGGAAT CCTATATGCA 
GCCAGAGATG TGACAGCCAC CGTGGACAGC CTGCCACTCA TCACAGCCTC CATTCTCAGT 
AAGAAACTCG TGGAGGGGCT GTCCGCTCTG GTGGTGGACG TTAAGTTCGG AGGGGCCGCC 
GTCTTCCCCA ACCAGGAGCA GGCCCGGGAG CTGGCAAAGA CGCTGGTTGG CGTGGGAGCC 
AGCCTAGGGC TTCGGGTCGC GGCAGCGCTG ACCGCCATGG ACAAGCCCCT GGGTCGCTGC 
GTGGGCCACG CCCTGGAGGT GGAGGAGGCG CTGCTCTGCA TGGACGGCGC AGGCCCGCCA 
GACTTAAGGG ACCTGGTCAC CACGCTCGGG GGCGCCCTGC TCTGGCTCAG CGGACACGCG 
GGGACTCAGG CCCAGGGCGC TGCCCGGGTG GCCGCGGCGC TGGACGACGG CTCGGCCCTT 
GGCCGCTTCG AGCGGATGCT GGCGGCGCAG GGCGTGGATC CCGGTCTGGC CCGAGCCCTG 
TGCTCGGGAA GTCCCGCAGA ACGCCGGCAG CTGCTGCCTC GCGCCCGGGA GCAGGAGGAG 
CTGCTGGCGC CCGCAGATGG CACCGTGGAG CTGGTCCGGG CGCTGCCGCT GGCGCTGGTG 
CTGCACGAGC TCGGGGCCGG GCGCAGCCGC GCTGGGGAGC CGCTCCGCCT GGGGGTGGGC 
GCAGAGCTGC TGGTCGACGT GGGTCAGAGG CTGCGCCGTG GGACCCCCTG GCTCCGCGTG 
CACCGGGACG GCCCCGCGCT CAGCGGCCCG CAGAGCCGCG CCCTGCAGGA GGCGCTCGTA 
CTCTCCGACC GCGCGCCATT CGCCGCCCCC TCGCCCTTCG CAGAGCTCGT TCTGCCGCCG 
CAGCAATAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu23077
Clone ID Related Accession (Same CDS sequence) NM_001257989.1
Accession Version NM_001257989.1 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 1464bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2019-08-12
Organism Homo sapiens(human)
Product thymidine phosphorylase isoform 2
Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from U62317.2, AK225269.1, BC052211.2 and BM744178.1. Transcript Variant: This variant (5) uses alternate splice sites in the 5' UTR and the 3' coding region, compared to variant 1. The resulting isoform (2) has an additional segment in the C-terminal region, compared to isoform 1. It is not known whether this isoform (2) is proteolytically processed in the same manner as isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK225269.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968540, SAMEA2142680 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: inferred from homology ##RefSeq-Attributes-END## 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
ATGGCAGCCT TGATGACCCC GGGAACCGGG GCCCCACCCG CGCCTGGTGA CTTCTCCGGG 
GAAGGGAGCC AGGGACTTCC CGACCCTTCG CCAGAGCCCA AGCAGCTCCC GGAGCTGATC 
CGCATGAAGC GAGACGGAGG CCGCCTGAGC GAAGCGGACA TCAGGGGCTT CGTGGCCGCT 
GTGGTGAATG GGAGCGCGCA GGGCGCACAG ATCGGGGCCA TGCTGATGGC CATCCGACTT 
CGGGGCATGG ATCTGGAGGA GACCTCGGTG CTGACCCAGG CCCTGGCTCA GTCGGGACAG 
CAGCTGGAGT GGCCAGAGGC CTGGCGCCAG CAGCTTGTGG ACAAGCATTC CACAGGGGGT 
GTGGGTGACA AGGTCAGCCT GGTCCTCGCA CCTGCCCTGG CGGCATGTGG CTGCAAGGTG 
CCAATGATCA GCGGACGTGG TCTGGGGCAC ACAGGAGGCA CCTTGGATAA GCTGGAGTCT 
ATTCCTGGAT TCAATGTCAT CCAGAGCCCA GAGCAGATGC AAGTGCTGCT GGACCAGGCG 
GGCTGCTGTA TCGTGGGTCA GAGTGAGCAG CTGGTTCCTG CGGACGGAAT CCTATATGCA 
GCCAGAGATG TGACAGCCAC CGTGGACAGC CTGCCACTCA TCACAGCCTC CATTCTCAGT 
AAGAAACTCG TGGAGGGGCT GTCCGCTCTG GTGGTGGACG TTAAGTTCGG AGGGGCCGCC 
GTCTTCCCCA ACCAGGAGCA GGCCCGGGAG CTGGCAAAGA CGCTGGTTGG CGTGGGAGCC 
AGCCTAGGGC TTCGGGTCGC GGCAGCGCTG ACCGCCATGG ACAAGCCCCT GGGTCGCTGC 
GTGGGCCACG CCCTGGAGGT GGAGGAGGCG CTGCTCTGCA TGGACGGCGC AGGCCCGCCA 
GACTTAAGGG ACCTGGTCAC CACGCTCGGG GGCGCCCTGC TCTGGCTCAG CGGACACGCG 
GGGACTCAGG CCCAGGGCGC TGCCCGGGTG GCCGCGGCGC TGGACGACGG CTCGGCCCTT 
GGCCGCTTCG AGCGGATGCT GGCGGCGCAG GGCGTGGATC CCGGTCTGGC CCGAGCCCTG 
TGCTCGGGAA GTCCCGCAGA ACGCCGGCAG CTGCTGCCTC GCGCCCGGGA GCAGGAGGAG 
CTGCTGGCGC CCGCAGATGC CCCTCTCCCC GCAGGCACCG TGGAGCTGGT CCGGGCGCTG 
CCGCTGGCGC TGGTGCTGCA CGAGCTCGGG GCCGGGCGCA GCCGCGCTGG GGAGCCGCTC 
CGCCTGGGGG TGGGCGCAGA GCTGCTGGTC GACGTGGGTC AGAGGCTGCG CCGTGGGACC 
CCCTGGCTCC GCGTGCACCG GGACGGCCCC GCGCTCAGCG GCCCGCAGAG CCGCGCCCTG 
CAGGAGGCGC TCGTACTCTC CGACCGCGCG CCATTCGCCG CCCCCTCGCC CTTCGCAGAG 
CTCGTTCTGC CGCCGCAGCA ATAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001244918.1
CDS193..1656
Translation

Target ORF information:

RefSeq Version NM_001257989.1
Organism Homo sapiens(human)
Definition Homo sapiens thymidine phosphorylase (TYMP), transcript variant 5, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 NM_001257989.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
ATGGCAGCCT TGATGACCCC GGGAACCGGG GCCCCACCCG CGCCTGGTGA CTTCTCCGGG 
GAAGGGAGCC AGGGACTTCC CGACCCTTCG CCAGAGCCCA AGCAGCTCCC GGAGCTGATC 
CGCATGAAGC GAGACGGAGG CCGCCTGAGC GAAGCGGACA TCAGGGGCTT CGTGGCCGCT 
GTGGTGAATG GGAGCGCGCA GGGCGCACAG ATCGGGGCCA TGCTGATGGC CATCCGACTT 
CGGGGCATGG ATCTGGAGGA GACCTCGGTG CTGACCCAGG CCCTGGCTCA GTCGGGACAG 
CAGCTGGAGT GGCCAGAGGC CTGGCGCCAG CAGCTTGTGG ACAAGCATTC CACAGGGGGT 
GTGGGTGACA AGGTCAGCCT GGTCCTCGCA CCTGCCCTGG CGGCATGTGG CTGCAAGGTG 
CCAATGATCA GCGGACGTGG TCTGGGGCAC ACAGGAGGCA CCTTGGATAA GCTGGAGTCT 
ATTCCTGGAT TCAATGTCAT CCAGAGCCCA GAGCAGATGC AAGTGCTGCT GGACCAGGCG 
GGCTGCTGTA TCGTGGGTCA GAGTGAGCAG CTGGTTCCTG CGGACGGAAT CCTATATGCA 
GCCAGAGATG TGACAGCCAC CGTGGACAGC CTGCCACTCA TCACAGCCTC CATTCTCAGT 
AAGAAACTCG TGGAGGGGCT GTCCGCTCTG GTGGTGGACG TTAAGTTCGG AGGGGCCGCC 
GTCTTCCCCA ACCAGGAGCA GGCCCGGGAG CTGGCAAAGA CGCTGGTTGG CGTGGGAGCC 
AGCCTAGGGC TTCGGGTCGC GGCAGCGCTG ACCGCCATGG ACAAGCCCCT GGGTCGCTGC 
GTGGGCCACG CCCTGGAGGT GGAGGAGGCG CTGCTCTGCA TGGACGGCGC AGGCCCGCCA 
GACTTAAGGG ACCTGGTCAC CACGCTCGGG GGCGCCCTGC TCTGGCTCAG CGGACACGCG 
GGGACTCAGG CCCAGGGCGC TGCCCGGGTG GCCGCGGCGC TGGACGACGG CTCGGCCCTT 
GGCCGCTTCG AGCGGATGCT GGCGGCGCAG GGCGTGGATC CCGGTCTGGC CCGAGCCCTG 
TGCTCGGGAA GTCCCGCAGA ACGCCGGCAG CTGCTGCCTC GCGCCCGGGA GCAGGAGGAG 
CTGCTGGCGC CCGCAGATGC CCCTCTCCCC GCAGGCACCG TGGAGCTGGT CCGGGCGCTG 
CCGCTGGCGC TGGTGCTGCA CGAGCTCGGG GCCGGGCGCA GCCGCGCTGG GGAGCCGCTC 
CGCCTGGGGG TGGGCGCAGA GCTGCTGGTC GACGTGGGTC AGAGGCTGCG CCGTGGGACC 
CCCTGGCTCC GCGTGCACCG GGACGGCCCC GCGCTCAGCG GCCCGCAGAG CCGCGCCCTG 
CAGGAGGCGC TCGTACTCTC CGACCGCGCG CCATTCGCCG CCCCCTCGCC CTTCGCAGAG 
CTCGTTCTGC CGCCGCAGCA ATAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

PubMed

book

Thymidine phosphorylase promotes metastasis and serves as a marker of poor prognosis in hepatocellular carcinoma.
Laboratory investigation; a journal of technical methods and pathology97(8)903-912(2017 Aug)
Zhang Q,Zhang Y,Hu X,Qin Y,Zhong W,Meng J,Xiao T,Zhang C,Li M,Chen S,Liu H,Liu Y,Sun T,Yang C


book

Poor Prognosis Associated with High Levels of Thymidine Phosphorylase and Thrombocytosis in Patients with Renal Cell Carcinoma.
Urologia internationalis98(2)162-168(2017)
Huang X,Wang L,Chen Y,Zheng X,Wang X


book

Pinpointing a Factor in Myeloma Bone Disease.
Cancer discovery6(11)1201-1202(2016 11)

book

Thymidine phosphorylase exerts complex effects on bone resorption and formation in myeloma.
Science translational medicine8(353)353ra113(2016 Aug)
Liu H,Liu Z,Du J,He J,Lin P,Amini B,Starbuck MW,Novane N,Shah JJ,Davis RE,Hou J,Gagel RF,Yang J