VWDE cDNA ORF clone, Homo sapiens(human)

  • Gene

  • Clones

  • gRNAs

The following VWDE gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the VWDE cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

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***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OHu31537 NM_001135924.3
Latest version!
Homo sapiens von Willebrand factor D and EGF domains (VWDE), transcript variant 1, mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK
$659.50-$923.30
$1319.00
OHu102142 NM_001346972.1
Latest version!
Homo sapiens von Willebrand factor D and EGF domains (VWDE), transcript variant 2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $727.30
$1039.00
OHu97082 NM_001346973.1
Latest version!
Homo sapiens von Willebrand factor D and EGF domains (VWDE), transcript variant 3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OHu97082 XM_017011829.1
Latest version!
Homo sapiens von Willebrand factor D and EGF domains (VWDE), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OHu97083 XM_017011830.1
Latest version!
Homo sapiens von Willebrand factor D and EGF domains (VWDE), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OHu41093 XM_005249641.1
Latest version!
Homo sapiens von Willebrand factor D and EGF domains (VWDE), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $559.30
$799.00
OHu78060 XM_011515174.1
Latest version!
Homo sapiens von Willebrand factor D and EGF domains (VWDE), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $951.30
$1359.00
OHu78061 XM_011515175.1
Latest version!
Homo sapiens von Willebrand factor D and EGF domains (VWDE), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $811.30
$1159.00
View Old Accession Versions >>
OHu31537 NM_001135924.2 Homo sapiens von Willebrand factor D and EGF domains (VWDE), transcript variant 1, mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK
$659.50-$923.30
$1319.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OHu31537
    Clone ID Related Accession (Same CDS sequence) NM_001135924.3 , NM_001135924.2
    Accession Version NM_001135924.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4773bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-04-30
    Organism Homo sapiens(human)
    Product von Willebrand factor D and EGF domain-containing protein isoform 1 precursor
    Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC013470.10 and AC007321.2. On Nov 22, 2018 this sequence version replaced NM_001135924.2. Transcript Variant: This variant (1) encodes the longest isoform (1). Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000275358.8/ ENSP00000275358.3 RefSeq Select criteria :: based on conservation, expression ##RefSeq-Attributes-END## COMPLETENESS: full length.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    ATGCCTGGCG GAGCCTGCGT GCTGGTGATC GCGCTGATGT TCCTGGCCTG GGGGGAAGCT 
    CAGGAGTGCT CTCCTGGGGG ACACCAGTTT CTTCGGAGTC CTTATAGAAG TGTCCGTTTT
    GACTCATGGC ACCTCCAGCA GTCAGCTGTT CAAGACCTAA TATGTGACCA TTCCCTCTCC
    CCTGGATGGT ATAGATTTCT CATCCTTGAC AGACCTGCCG AGATGCCAAC CAAATGTGTT
    GAGATGAACC ATTGTGGAAC TCAGGCCCCC ATCTGGCTGT CTCTGAGAGA TTCAGAAACA
    CTGCCATCTC CTGGGGAGAT CAAGCAATTG ACAGCTTGTG CAACATGGCA GTTTTTGTTC
    AGCACTACAA AAGACTGCTG TCTCTTTCAA ATCCCAGTGT CTGTAAGAAA CTGTGGGAAC
    TTTTCTGTAT ACTTACTACA ACCAACTCAG GGATGTATGG GCTACTGTGC GGAAGCTATT
    TCTGATGCAC GATTACACCC ATGTGGTTCT GATGAAACTG AAACAGGAGG TGATTGTGTT
    CGTCAGCTGG CTGCCTCATT GCCACCTCCA CCTGCAGGAA GGCCAGAGGT TCTGGTGGAG
    TTGATTGAGT CCAGGCTTTT CTGTAGGTGT TCTTTTGATG TTCCCGCTAC AAAAAACTCA
    GTGGGATTTC ACATAGCTTG GTCTAGGCTT TCTTCTCAAG AAGTCAAAGA GGAGCTGACA
    CAAGAGACCA CAGTTCAGGC ATTCTCTCTT TTAGAACTTG ATGGCATAAA TCTCAGACTT
    GGAGACAGGA TATTCTGCAG CGCTTCTGTC TTTTTCTTGG AGAATCCTCA TGTACAAAGT
    GTAGCCATCG AAAGCCAAGA ATTTTTTGCA GGCTTTAAGC TACAGCCTGA ATTGAGCACT
    ATATCAGAGG ATGGGAAAGA ATACTACCTG AGGATAGAAA GCACAGTTCC TATTATTTGT
    TCTGAATTTA GTGAGCTTGA TCAAGAATGC AAAATCTCAT TAAAACTGAA AACTATTGGT
    CAAGGTAGAG AGCACCTAGG CTTAAATTTG GCACTGTCTT CCTGTCATGT GGACCTTCTC
    CAGACATCTT CCTGTGCTAA TGGAACCTGT AGCCACACTT TTGTGTACTA CACTGCTGTC
    ACAGATTTTT CTCGAGATGG AGATAGAGTC TCAAACATTG TAGTGCAACC AATAGTTAAT
    GAGGATTTCC TGTGGAACAA CTACATTCCA GACAGCATCC AGATCAAAGT AAAGGATGTC
    CCAACTGCTT ACTGCTATAC ATTTACTGAC CCACATATAA TTACATTTGA TGGCAGGGTA
    TATGATAATT TCAAGACTGG AACATTTGTG CTTTATAAGA GTATGTCACG TGATTTTGAA
    GTCCATGTAC GTCAGTGGGA CTGCAGAAGC CTTCACTATC CAGTGTCATG TAATTGTGGG
    TTTGTTGCCC AGGAAGGAGG TGATATAGTT ACTTTTGATA TGTGCAATGG TCAGCTACGT
    GAATCACAAC CATATTTGTT CATAAAAAGC CAAGATGTAA CCAGGAATAT CAAGATAAGT
    GAATCTTACT TAGGAAGAAA AGTCACAATC TGGTTTTCTT CTGGGGCATT TATCCGTGCT
    GATCTTGGTG AATGGGGCAT GAGTCTAACG ATCAGAGCCC CTAGTGTAGA TTACAGAAAC
    ACTCTGGGAC TTTGTGGAAC CTTTGATGAA AATCCGGAAA ATGATTTCCA TGACAAAAAT
    GGGATGCAAA TTGATCAAAA TTTTAACAAT TATGTTGCTT TTATTAATGA ATGGAGGATT
    TTACCAGGAA AAAGCATGTC TGACACACTG CCAGTTTCTA TGACATCACC TGGAAAGCCA
    TCCTATTGTA GCTGTTCATT GGACACTGCA GCGTATCCGT CTTCCGAAGA TCTGGACAGT
    GTTTCTCGAT CAGAAATTGC CTTGGGTTGC AAAGACCTCA ATCATGTCAG CTTATCTTCT
    TTGATACCAG AACTAGATGT CACCTCCGAA TATATTAATT CAGACACTCT TGTCAGAGAG
    ATAAACAAGC ATACATCTCC AGAAGAATAT AATCTAAATT TATTTCTGCA AGAAAAAAAA
    CACATAAACC TGACTAAACT CGGCTTAAAT GTACAAAAAC ATCCTGGAAA TGAGAAAGAA
    GATTCACTAC AATATTTGGC CAATAAGAAA TATACACAAG GCCGGGGAAG CCACAGCCAA
    GAAATGAGGT ACAATCGACA AAACAGATGG AAACGGCAGA ACTTTCATGA GTTTCCTCCT
    TTGTTTGCTT TCCCGAGTCT CAGCCAAACG GATCTGGAAG AACTTACTTA TTTTTTCCCA
    GAGGACCATG CTGAGGATGT ACAGCAAGAG TTTTTCCCCT CTTGGCCCAC TCCCTCTGGC
    CTCACCGAGT ATAGCACCTT GACCCTCTGT CAGGAGACTC TAGCCAACTC CAGCATAGGA
    AGGCTGTGTC TTGCTTTTCT TGGCAAGAGA TTAGACAGTG TTATAGAGAT GTGTGTGAAG
    GATGTTCTGT TAAAAGATGA TCTTAGTTGG GCAGAAGCAG GTGTGGCCCT TTTAGAAAAT
    GAATGTGAAA AGAGGATTGT GGAGGAGGGG AAATATAACA CAGAAGAGTA TGGCACATCA
    ATTGAAGACA TTCTCTCAGT ATTAAAATGC CCCAATTTAT GCAGCGGCAA TGGGCAGTGC
    ATGGAATGGG GGTGTGCGTG TTCCCCAAGC TTTAGTTCCT ATGACTGCAG TGATTCCTAT
    GACAAAGCTC CTGAAATTAC AGAGCTTGGG AATGCTGGAT TCTGTGATGT TCAAAAATAT
    AATTGTATGA TGGTGAGAGT TTTTGGCAAA GGCTTCAAAG AATTACCTTC AATTAAATGT
    GAAGTTACTA AGCTACAGTA TAATAGCAGT GAATGGATGC CTGGAGAACC CATCTATACA
    CAGACTGTTT TCCACAATAG CAGAGCTGTT GATTGTCAGC TGCCCACTGA TGTTCAGCAG
    TTTGATACCA TGGATCTGGT GGGTGGGAAA CCAACTGGGA AGTGGCAGTT GAAGGTATCT
    AATGATGGTT ATAAATTCAG TAATCCCAAA ATAACGGTCA TATATGATGG TGCTTGCCAA
    GTTTGTGGTC TCTATAAAAA TGACTCATGT ACTATAAAGG AAAATGTTTG CATTATTGAT
    GGACTCTGCT ATGTTGAAGG AGACAAAAAT CCAACCAGCC CTTGTTTGAT TTGTAGACCC
    AAAATTTCAA GATTTACTTG GTCATTTTTA GAAAACAACC AGCCCCCAGT GATTCAAGCA
    TTGCAAGACA AATTACAGAC ATTTTATGGT GAAAACTTTG AGTATCAGTT CGTGGCCTTC
    GATCCAGAAG GTTCTGACAT CCATTTTACG TTGGACTCTG GTCCTGAAGG GGCAAGTGTT
    TCCTCTGCAG GGCTTTTTAT GTGGAAAACA GATTTACTAA CTACCCAACA AATTACTGTA
    CGCCTTAATG ATGACTGCGA TGCTGAAACT AGAGTCACGA TTGAGGTGAC TGTGAAGTCT
    TGTGATTGCT TGAATGGTGG ATCATGTGTA TCTGATAGGA ACTTTTCTCC AGGGAGTGGA
    GTGTACCTGT GTGTCTGCTT GCCTGGCTTC CATGGCAGCC TTTGTGAAGT GGACATTAGT
    GGGTGCCAAT CCAACCCTTG TGGTCTTGGC AGCTATATTA GTGGTTTTCA CAGTTATTCC
    TGTGATTGTC CACCTGAGCT CAAAGTTGAA ACACAGTTTG TAAATCAATT TACTACACAA
    ACTGTGGTTC TCACAAGATC TGATAAAAGT GTAAATAAAG AAGAGGATGA TAAAAATGCC
    CAAGGGAGAA AGAGGCATGT TAAGCCAACA AGTGGAAATG CCTTCACCAT TTGTAAATAT
    CCATGTGGAA AAAGTAGGGA GTGTGTTGCC CCAAACATCT GCAAATGTAA ACCTGGTTAC
    ATTGGTTCTA ACTGCCAAAC TGCTCTTTGT GACCCTGATT GCAAAAACCA TGGAAAATGT
    ATTAAGCCTA ACATTTGTCA GTGTCTTCCA GGACATGGTG GAGCAACCTG TGATGAAGAA
    CATTGTAACC CACCTTGTCA ACATGGTGGC ACATGCTTGG CTGGGAATCT CTGCACTTGT
    CCTTATGGTT TTGTAGGACC AAGGTGTGAA ACAATGGTTT GTAACAGGCA CTGTGAAAAT
    GGAGGCCAGT GTCTTACACC AGATATTTGC CAGTGCAAAC CTGGCTGGTA TGGACCCACC
    TGTAGTACAG CTTTGTGCGA CCCTGTCTGC CTCAATGGTG GTTCGTGTAA TAAGCCAAAT
    ACTTGCCTCT GTCCAAATGG ATTCTTTGGG GAACACTGTC AGAATGCTTT CTGTCACCCT
    CCCTGTAAGA ATGGTGGCCA CTGCATGAGA AATAATGTGT GCGTCTGTCG TGAAGGATAC
    ACTGGTAGGA GATTCCAAAA AAGCATCTGT GATCCTACGT GCATGAATGG AGGAAAATGT
    GTGGGACCAA GCACTTGCTC TTGTCCTTCT GGTTGGAGTG GGAAACGATG CAACACACCT
    ATCTGCTTGC AGAAATGTAA AAACGGTGGT GAATGCATTG CGCCCAGCAT ATGCCATTGT
    CCTTCCTCCT GGGAAGGAGT GCGGTGTCAA ATACCAATTT GCAACCCAAA ATGTCTTTAT
    GGAGGCAGAT GCATATTTCC CAATGTGTGT TCCTGCCGCA CTGAATACTC TGGAGTCAAA
    TGTGAGAAGA AAATACAGAT AAGACGCCAT TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001129396.1
    CDS150..4922
    Translation

    Target ORF information:

    RefSeq Version NM_001135924.3
    Organism Homo sapiens(human)
    Definition Homo sapiens von Willebrand factor D and EGF domains (VWDE), transcript variant 1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001135924.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    ATGCCTGGCG GAGCCTGCGT GCTGGTGATC GCGCTGATGT TCCTGGCCTG GGGGGAAGCT 
    CAGGAGTGCT CTCCTGGGGG ACACCAGTTT CTTCGGAGTC CTTATAGAAG TGTCCGTTTT
    GACTCATGGC ACCTCCAGCA GTCAGCTGTT CAAGACCTAA TATGTGACCA TTCCCTCTCC
    CCTGGATGGT ATAGATTTCT CATCCTTGAC AGACCTGCCG AGATGCCAAC CAAATGTGTT
    GAGATGAACC ATTGTGGAAC TCAGGCCCCC ATCTGGCTGT CTCTGAGAGA TTCAGAAACA
    CTGCCATCTC CTGGGGAGAT CAAGCAATTG ACAGCTTGTG CAACATGGCA GTTTTTGTTC
    AGCACTACAA AAGACTGCTG TCTCTTTCAA ATCCCAGTGT CTGTAAGAAA CTGTGGGAAC
    TTTTCTGTAT ACTTACTACA ACCAACTCAG GGATGTATGG GCTACTGTGC GGAAGCTATT
    TCTGATGCAC GATTACACCC ATGTGGTTCT GATGAAACTG AAACAGGAGG TGATTGTGTT
    CGTCAGCTGG CTGCCTCATT GCCACCTCCA CCTGCAGGAA GGCCAGAGGT TCTGGTGGAG
    TTGATTGAGT CCAGGCTTTT CTGTAGGTGT TCTTTTGATG TTCCCGCTAC AAAAAACTCA
    GTGGGATTTC ACATAGCTTG GTCTAGGCTT TCTTCTCAAG AAGTCAAAGA GGAGCTGACA
    CAAGAGACCA CAGTTCAGGC ATTCTCTCTT TTAGAACTTG ATGGCATAAA TCTCAGACTT
    GGAGACAGGA TATTCTGCAG CGCTTCTGTC TTTTTCTTGG AGAATCCTCA TGTACAAAGT
    GTAGCCATCG AAAGCCAAGA ATTTTTTGCA GGCTTTAAGC TACAGCCTGA ATTGAGCACT
    ATATCAGAGG ATGGGAAAGA ATACTACCTG AGGATAGAAA GCACAGTTCC TATTATTTGT
    TCTGAATTTA GTGAGCTTGA TCAAGAATGC AAAATCTCAT TAAAACTGAA AACTATTGGT
    CAAGGTAGAG AGCACCTAGG CTTAAATTTG GCACTGTCTT CCTGTCATGT GGACCTTCTC
    CAGACATCTT CCTGTGCTAA TGGAACCTGT AGCCACACTT TTGTGTACTA CACTGCTGTC
    ACAGATTTTT CTCGAGATGG AGATAGAGTC TCAAACATTG TAGTGCAACC AATAGTTAAT
    GAGGATTTCC TGTGGAACAA CTACATTCCA GACAGCATCC AGATCAAAGT AAAGGATGTC
    CCAACTGCTT ACTGCTATAC ATTTACTGAC CCACATATAA TTACATTTGA TGGCAGGGTA
    TATGATAATT TCAAGACTGG AACATTTGTG CTTTATAAGA GTATGTCACG TGATTTTGAA
    GTCCATGTAC GTCAGTGGGA CTGCAGAAGC CTTCACTATC CAGTGTCATG TAATTGTGGG
    TTTGTTGCCC AGGAAGGAGG TGATATAGTT ACTTTTGATA TGTGCAATGG TCAGCTACGT
    GAATCACAAC CATATTTGTT CATAAAAAGC CAAGATGTAA CCAGGAATAT CAAGATAAGT
    GAATCTTACT TAGGAAGAAA AGTCACAATC TGGTTTTCTT CTGGGGCATT TATCCGTGCT
    GATCTTGGTG AATGGGGCAT GAGTCTAACG ATCAGAGCCC CTAGTGTAGA TTACAGAAAC
    ACTCTGGGAC TTTGTGGAAC CTTTGATGAA AATCCGGAAA ATGATTTCCA TGACAAAAAT
    GGGATGCAAA TTGATCAAAA TTTTAACAAT TATGTTGCTT TTATTAATGA ATGGAGGATT
    TTACCAGGAA AAAGCATGTC TGACACACTG CCAGTTTCTA TGACATCACC TGGAAAGCCA
    TCCTATTGTA GCTGTTCATT GGACACTGCA GCGTATCCGT CTTCCGAAGA TCTGGACAGT
    GTTTCTCGAT CAGAAATTGC CTTGGGTTGC AAAGACCTCA ATCATGTCAG CTTATCTTCT
    TTGATACCAG AACTAGATGT CACCTCCGAA TATATTAATT CAGACACTCT TGTCAGAGAG
    ATAAACAAGC ATACATCTCC AGAAGAATAT AATCTAAATT TATTTCTGCA AGAAAAAAAA
    CACATAAACC TGACTAAACT CGGCTTAAAT GTACAAAAAC ATCCTGGAAA TGAGAAAGAA
    GATTCACTAC AATATTTGGC CAATAAGAAA TATACACAAG GCCGGGGAAG CCACAGCCAA
    GAAATGAGGT ACAATCGACA AAACAGATGG AAACGGCAGA ACTTTCATGA GTTTCCTCCT
    TTGTTTGCTT TCCCGAGTCT CAGCCAAACG GATCTGGAAG AACTTACTTA TTTTTTCCCA
    GAGGACCATG CTGAGGATGT ACAGCAAGAG TTTTTCCCCT CTTGGCCCAC TCCCTCTGGC
    CTCACCGAGT ATAGCACCTT GACCCTCTGT CAGGAGACTC TAGCCAACTC CAGCATAGGA
    AGGCTGTGTC TTGCTTTTCT TGGCAAGAGA TTAGACAGTG TTATAGAGAT GTGTGTGAAG
    GATGTTCTGT TAAAAGATGA TCTTAGTTGG GCAGAAGCAG GTGTGGCCCT TTTAGAAAAT
    GAATGTGAAA AGAGGATTGT GGAGGAGGGG AAATATAACA CAGAAGAGTA TGGCACATCA
    ATTGAAGACA TTCTCTCAGT ATTAAAATGC CCCAATTTAT GCAGCGGCAA TGGGCAGTGC
    ATGGAATGGG GGTGTGCGTG TTCCCCAAGC TTTAGTTCCT ATGACTGCAG TGATTCCTAT
    GACAAAGCTC CTGAAATTAC AGAGCTTGGG AATGCTGGAT TCTGTGATGT TCAAAAATAT
    AATTGTATGA TGGTGAGAGT TTTTGGCAAA GGCTTCAAAG AATTACCTTC AATTAAATGT
    GAAGTTACTA AGCTACAGTA TAATAGCAGT GAATGGATGC CTGGAGAACC CATCTATACA
    CAGACTGTTT TCCACAATAG CAGAGCTGTT GATTGTCAGC TGCCCACTGA TGTTCAGCAG
    TTTGATACCA TGGATCTGGT GGGTGGGAAA CCAACTGGGA AGTGGCAGTT GAAGGTATCT
    AATGATGGTT ATAAATTCAG TAATCCCAAA ATAACGGTCA TATATGATGG TGCTTGCCAA
    GTTTGTGGTC TCTATAAAAA TGACTCATGT ACTATAAAGG AAAATGTTTG CATTATTGAT
    GGACTCTGCT ATGTTGAAGG AGACAAAAAT CCAACCAGCC CTTGTTTGAT TTGTAGACCC
    AAAATTTCAA GATTTACTTG GTCATTTTTA GAAAACAACC AGCCCCCAGT GATTCAAGCA
    TTGCAAGACA AATTACAGAC ATTTTATGGT GAAAACTTTG AGTATCAGTT CGTGGCCTTC
    GATCCAGAAG GTTCTGACAT CCATTTTACG TTGGACTCTG GTCCTGAAGG GGCAAGTGTT
    TCCTCTGCAG GGCTTTTTAT GTGGAAAACA GATTTACTAA CTACCCAACA AATTACTGTA
    CGCCTTAATG ATGACTGCGA TGCTGAAACT AGAGTCACGA TTGAGGTGAC TGTGAAGTCT
    TGTGATTGCT TGAATGGTGG ATCATGTGTA TCTGATAGGA ACTTTTCTCC AGGGAGTGGA
    GTGTACCTGT GTGTCTGCTT GCCTGGCTTC CATGGCAGCC TTTGTGAAGT GGACATTAGT
    GGGTGCCAAT CCAACCCTTG TGGTCTTGGC AGCTATATTA GTGGTTTTCA CAGTTATTCC
    TGTGATTGTC CACCTGAGCT CAAAGTTGAA ACACAGTTTG TAAATCAATT TACTACACAA
    ACTGTGGTTC TCACAAGATC TGATAAAAGT GTAAATAAAG AAGAGGATGA TAAAAATGCC
    CAAGGGAGAA AGAGGCATGT TAAGCCAACA AGTGGAAATG CCTTCACCAT TTGTAAATAT
    CCATGTGGAA AAAGTAGGGA GTGTGTTGCC CCAAACATCT GCAAATGTAA ACCTGGTTAC
    ATTGGTTCTA ACTGCCAAAC TGCTCTTTGT GACCCTGATT GCAAAAACCA TGGAAAATGT
    ATTAAGCCTA ACATTTGTCA GTGTCTTCCA GGACATGGTG GAGCAACCTG TGATGAAGAA
    CATTGTAACC CACCTTGTCA ACATGGTGGC ACATGCTTGG CTGGGAATCT CTGCACTTGT
    CCTTATGGTT TTGTAGGACC AAGGTGTGAA ACAATGGTTT GTAACAGGCA CTGTGAAAAT
    GGAGGCCAGT GTCTTACACC AGATATTTGC CAGTGCAAAC CTGGCTGGTA TGGACCCACC
    TGTAGTACAG CTTTGTGCGA CCCTGTCTGC CTCAATGGTG GTTCGTGTAA TAAGCCAAAT
    ACTTGCCTCT GTCCAAATGG ATTCTTTGGG GAACACTGTC AGAATGCTTT CTGTCACCCT
    CCCTGTAAGA ATGGTGGCCA CTGCATGAGA AATAATGTGT GCGTCTGTCG TGAAGGATAC
    ACTGGTAGGA GATTCCAAAA AAGCATCTGT GATCCTACGT GCATGAATGG AGGAAAATGT
    GTGGGACCAA GCACTTGCTC TTGTCCTTCT GGTTGGAGTG GGAAACGATG CAACACACCT
    ATCTGCTTGC AGAAATGTAA AAACGGTGGT GAATGCATTG CGCCCAGCAT ATGCCATTGT
    CCTTCCTCCT GGGAAGGAGT GCGGTGTCAA ATACCAATTT GCAACCCAAA ATGTCTTTAT
    GGAGGCAGAT GCATATTTCC CAATGTGTGT TCCTGCCGCA CTGAATACTC TGGAGTCAAA
    TGTGAGAAGA AAATACAGAT AAGACGCCAT TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu31537
    Clone ID Related Accession (Same CDS sequence) NM_001135924.3 , NM_001135924.2
    Accession Version NM_001135924.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4773bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-09
    Organism Homo sapiens(human)
    Product von Willebrand factor D and EGF domain-containing protein isoform 1 precursor
    Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC013470.10 and AC007321.2. On Nov 12, 2016 this sequence version replaced NM_001135924.1. Transcript Variant: This variant (1) encodes the longest isoform (1). Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    ATGCCTGGCG GAGCCTGCGT GCTGGTGATC GCGCTGATGT TCCTGGCCTG GGGGGAAGCT 
    CAGGAGTGCT CTCCTGGGGG ACACCAGTTT CTTCGGAGTC CTTATAGAAG TGTCCGTTTT
    GACTCATGGC ACCTCCAGCA GTCAGCTGTT CAAGACCTAA TATGTGACCA TTCCCTCTCC
    CCTGGATGGT ATAGATTTCT CATCCTTGAC AGACCTGCCG AGATGCCAAC CAAATGTGTT
    GAGATGAACC ATTGTGGAAC TCAGGCCCCC ATCTGGCTGT CTCTGAGAGA TTCAGAAACA
    CTGCCATCTC CTGGGGAGAT CAAGCAATTG ACAGCTTGTG CAACATGGCA GTTTTTGTTC
    AGCACTACAA AAGACTGCTG TCTCTTTCAA ATCCCAGTGT CTGTAAGAAA CTGTGGGAAC
    TTTTCTGTAT ACTTACTACA ACCAACTCAG GGATGTATGG GCTACTGTGC GGAAGCTATT
    TCTGATGCAC GATTACACCC ATGTGGTTCT GATGAAACTG AAACAGGAGG TGATTGTGTT
    CGTCAGCTGG CTGCCTCATT GCCACCTCCA CCTGCAGGAA GGCCAGAGGT TCTGGTGGAG
    TTGATTGAGT CCAGGCTTTT CTGTAGGTGT TCTTTTGATG TTCCCGCTAC AAAAAACTCA
    GTGGGATTTC ACATAGCTTG GTCTAGGCTT TCTTCTCAAG AAGTCAAAGA GGAGCTGACA
    CAAGAGACCA CAGTTCAGGC ATTCTCTCTT TTAGAACTTG ATGGCATAAA TCTCAGACTT
    GGAGACAGGA TATTCTGCAG CGCTTCTGTC TTTTTCTTGG AGAATCCTCA TGTACAAAGT
    GTAGCCATCG AAAGCCAAGA ATTTTTTGCA GGCTTTAAGC TACAGCCTGA ATTGAGCACT
    ATATCAGAGG ATGGGAAAGA ATACTACCTG AGGATAGAAA GCACAGTTCC TATTATTTGT
    TCTGAATTTA GTGAGCTTGA TCAAGAATGC AAAATCTCAT TAAAACTGAA AACTATTGGT
    CAAGGTAGAG AGCACCTAGG CTTAAATTTG GCACTGTCTT CCTGTCATGT GGACCTTCTC
    CAGACATCTT CCTGTGCTAA TGGAACCTGT AGCCACACTT TTGTGTACTA CACTGCTGTC
    ACAGATTTTT CTCGAGATGG AGATAGAGTC TCAAACATTG TAGTGCAACC AATAGTTAAT
    GAGGATTTCC TGTGGAACAA CTACATTCCA GACAGCATCC AGATCAAAGT AAAGGATGTC
    CCAACTGCTT ACTGCTATAC ATTTACTGAC CCACATATAA TTACATTTGA TGGCAGGGTA
    TATGATAATT TCAAGACTGG AACATTTGTG CTTTATAAGA GTATGTCACG TGATTTTGAA
    GTCCATGTAC GTCAGTGGGA CTGCAGAAGC CTTCACTATC CAGTGTCATG TAATTGTGGG
    TTTGTTGCCC AGGAAGGAGG TGATATAGTT ACTTTTGATA TGTGCAATGG TCAGCTACGT
    GAATCACAAC CATATTTGTT CATAAAAAGC CAAGATGTAA CCAGGAATAT CAAGATAAGT
    GAATCTTACT TAGGAAGAAA AGTCACAATC TGGTTTTCTT CTGGGGCATT TATCCGTGCT
    GATCTTGGTG AATGGGGCAT GAGTCTAACG ATCAGAGCCC CTAGTGTAGA TTACAGAAAC
    ACTCTGGGAC TTTGTGGAAC CTTTGATGAA AATCCGGAAA ATGATTTCCA TGACAAAAAT
    GGGATGCAAA TTGATCAAAA TTTTAACAAT TATGTTGCTT TTATTAATGA ATGGAGGATT
    TTACCAGGAA AAAGCATGTC TGACACACTG CCAGTTTCTA TGACATCACC TGGAAAGCCA
    TCCTATTGTA GCTGTTCATT GGACACTGCA GCGTATCCGT CTTCCGAAGA TCTGGACAGT
    GTTTCTCGAT CAGAAATTGC CTTGGGTTGC AAAGACCTCA ATCATGTCAG CTTATCTTCT
    TTGATACCAG AACTAGATGT CACCTCCGAA TATATTAATT CAGACACTCT TGTCAGAGAG
    ATAAACAAGC ATACATCTCC AGAAGAATAT AATCTAAATT TATTTCTGCA AGAAAAAAAA
    CACATAAACC TGACTAAACT CGGCTTAAAT GTACAAAAAC ATCCTGGAAA TGAGAAAGAA
    GATTCACTAC AATATTTGGC CAATAAGAAA TATACACAAG GCCGGGGAAG CCACAGCCAA
    GAAATGAGGT ACAATCGACA AAACAGATGG AAACGGCAGA ACTTTCATGA GTTTCCTCCT
    TTGTTTGCTT TCCCGAGTCT CAGCCAAACG GATCTGGAAG AACTTACTTA TTTTTTCCCA
    GAGGACCATG CTGAGGATGT ACAGCAAGAG TTTTTCCCCT CTTGGCCCAC TCCCTCTGGC
    CTCACCGAGT ATAGCACCTT GACCCTCTGT CAGGAGACTC TAGCCAACTC CAGCATAGGA
    AGGCTGTGTC TTGCTTTTCT TGGCAAGAGA TTAGACAGTG TTATAGAGAT GTGTGTGAAG
    GATGTTCTGT TAAAAGATGA TCTTAGTTGG GCAGAAGCAG GTGTGGCCCT TTTAGAAAAT
    GAATGTGAAA AGAGGATTGT GGAGGAGGGG AAATATAACA CAGAAGAGTA TGGCACATCA
    ATTGAAGACA TTCTCTCAGT ATTAAAATGC CCCAATTTAT GCAGCGGCAA TGGGCAGTGC
    ATGGAATGGG GGTGTGCGTG TTCCCCAAGC TTTAGTTCCT ATGACTGCAG TGATTCCTAT
    GACAAAGCTC CTGAAATTAC AGAGCTTGGG AATGCTGGAT TCTGTGATGT TCAAAAATAT
    AATTGTATGA TGGTGAGAGT TTTTGGCAAA GGCTTCAAAG AATTACCTTC AATTAAATGT
    GAAGTTACTA AGCTACAGTA TAATAGCAGT GAATGGATGC CTGGAGAACC CATCTATACA
    CAGACTGTTT TCCACAATAG CAGAGCTGTT GATTGTCAGC TGCCCACTGA TGTTCAGCAG
    TTTGATACCA TGGATCTGGT GGGTGGGAAA CCAACTGGGA AGTGGCAGTT GAAGGTATCT
    AATGATGGTT ATAAATTCAG TAATCCCAAA ATAACGGTCA TATATGATGG TGCTTGCCAA
    GTTTGTGGTC TCTATAAAAA TGACTCATGT ACTATAAAGG AAAATGTTTG CATTATTGAT
    GGACTCTGCT ATGTTGAAGG AGACAAAAAT CCAACCAGCC CTTGTTTGAT TTGTAGACCC
    AAAATTTCAA GATTTACTTG GTCATTTTTA GAAAACAACC AGCCCCCAGT GATTCAAGCA
    TTGCAAGACA AATTACAGAC ATTTTATGGT GAAAACTTTG AGTATCAGTT CGTGGCCTTC
    GATCCAGAAG GTTCTGACAT CCATTTTACG TTGGACTCTG GTCCTGAAGG GGCAAGTGTT
    TCCTCTGCAG GGCTTTTTAT GTGGAAAACA GATTTACTAA CTACCCAACA AATTACTGTA
    CGCCTTAATG ATGACTGCGA TGCTGAAACT AGAGTCACGA TTGAGGTGAC TGTGAAGTCT
    TGTGATTGCT TGAATGGTGG ATCATGTGTA TCTGATAGGA ACTTTTCTCC AGGGAGTGGA
    GTGTACCTGT GTGTCTGCTT GCCTGGCTTC CATGGCAGCC TTTGTGAAGT GGACATTAGT
    GGGTGCCAAT CCAACCCTTG TGGTCTTGGC AGCTATATTA GTGGTTTTCA CAGTTATTCC
    TGTGATTGTC CACCTGAGCT CAAAGTTGAA ACACAGTTTG TAAATCAATT TACTACACAA
    ACTGTGGTTC TCACAAGATC TGATAAAAGT GTAAATAAAG AAGAGGATGA TAAAAATGCC
    CAAGGGAGAA AGAGGCATGT TAAGCCAACA AGTGGAAATG CCTTCACCAT TTGTAAATAT
    CCATGTGGAA AAAGTAGGGA GTGTGTTGCC CCAAACATCT GCAAATGTAA ACCTGGTTAC
    ATTGGTTCTA ACTGCCAAAC TGCTCTTTGT GACCCTGATT GCAAAAACCA TGGAAAATGT
    ATTAAGCCTA ACATTTGTCA GTGTCTTCCA GGACATGGTG GAGCAACCTG TGATGAAGAA
    CATTGTAACC CACCTTGTCA ACATGGTGGC ACATGCTTGG CTGGGAATCT CTGCACTTGT
    CCTTATGGTT TTGTAGGACC AAGGTGTGAA ACAATGGTTT GTAACAGGCA CTGTGAAAAT
    GGAGGCCAGT GTCTTACACC AGATATTTGC CAGTGCAAAC CTGGCTGGTA TGGACCCACC
    TGTAGTACAG CTTTGTGCGA CCCTGTCTGC CTCAATGGTG GTTCGTGTAA TAAGCCAAAT
    ACTTGCCTCT GTCCAAATGG ATTCTTTGGG GAACACTGTC AGAATGCTTT CTGTCACCCT
    CCCTGTAAGA ATGGTGGCCA CTGCATGAGA AATAATGTGT GCGTCTGTCG TGAAGGATAC
    ACTGGTAGGA GATTCCAAAA AAGCATCTGT GATCCTACGT GCATGAATGG AGGAAAATGT
    GTGGGACCAA GCACTTGCTC TTGTCCTTCT GGTTGGAGTG GGAAACGATG CAACACACCT
    ATCTGCTTGC AGAAATGTAA AAACGGTGGT GAATGCATTG CGCCCAGCAT ATGCCATTGT
    CCTTCCTCCT GGGAAGGAGT GCGGTGTCAA ATACCAATTT GCAACCCAAA ATGTCTTTAT
    GGAGGCAGAT GCATATTTCC CAATGTGTGT TCCTGCCGCA CTGAATACTC TGGAGTCAAA
    TGTGAGAAGA AAATACAGAT AAGACGCCAT TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001129396.1
    CDS226..4998
    Translation

    Target ORF information:

    RefSeq Version NM_001135924.2
    Organism Homo sapiens(human)
    Definition Homo sapiens von Willebrand factor D and EGF domains (VWDE), transcript variant 1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001135924.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    ATGCCTGGCG GAGCCTGCGT GCTGGTGATC GCGCTGATGT TCCTGGCCTG GGGGGAAGCT 
    CAGGAGTGCT CTCCTGGGGG ACACCAGTTT CTTCGGAGTC CTTATAGAAG TGTCCGTTTT
    GACTCATGGC ACCTCCAGCA GTCAGCTGTT CAAGACCTAA TATGTGACCA TTCCCTCTCC
    CCTGGATGGT ATAGATTTCT CATCCTTGAC AGACCTGCCG AGATGCCAAC CAAATGTGTT
    GAGATGAACC ATTGTGGAAC TCAGGCCCCC ATCTGGCTGT CTCTGAGAGA TTCAGAAACA
    CTGCCATCTC CTGGGGAGAT CAAGCAATTG ACAGCTTGTG CAACATGGCA GTTTTTGTTC
    AGCACTACAA AAGACTGCTG TCTCTTTCAA ATCCCAGTGT CTGTAAGAAA CTGTGGGAAC
    TTTTCTGTAT ACTTACTACA ACCAACTCAG GGATGTATGG GCTACTGTGC GGAAGCTATT
    TCTGATGCAC GATTACACCC ATGTGGTTCT GATGAAACTG AAACAGGAGG TGATTGTGTT
    CGTCAGCTGG CTGCCTCATT GCCACCTCCA CCTGCAGGAA GGCCAGAGGT TCTGGTGGAG
    TTGATTGAGT CCAGGCTTTT CTGTAGGTGT TCTTTTGATG TTCCCGCTAC AAAAAACTCA
    GTGGGATTTC ACATAGCTTG GTCTAGGCTT TCTTCTCAAG AAGTCAAAGA GGAGCTGACA
    CAAGAGACCA CAGTTCAGGC ATTCTCTCTT TTAGAACTTG ATGGCATAAA TCTCAGACTT
    GGAGACAGGA TATTCTGCAG CGCTTCTGTC TTTTTCTTGG AGAATCCTCA TGTACAAAGT
    GTAGCCATCG AAAGCCAAGA ATTTTTTGCA GGCTTTAAGC TACAGCCTGA ATTGAGCACT
    ATATCAGAGG ATGGGAAAGA ATACTACCTG AGGATAGAAA GCACAGTTCC TATTATTTGT
    TCTGAATTTA GTGAGCTTGA TCAAGAATGC AAAATCTCAT TAAAACTGAA AACTATTGGT
    CAAGGTAGAG AGCACCTAGG CTTAAATTTG GCACTGTCTT CCTGTCATGT GGACCTTCTC
    CAGACATCTT CCTGTGCTAA TGGAACCTGT AGCCACACTT TTGTGTACTA CACTGCTGTC
    ACAGATTTTT CTCGAGATGG AGATAGAGTC TCAAACATTG TAGTGCAACC AATAGTTAAT
    GAGGATTTCC TGTGGAACAA CTACATTCCA GACAGCATCC AGATCAAAGT AAAGGATGTC
    CCAACTGCTT ACTGCTATAC ATTTACTGAC CCACATATAA TTACATTTGA TGGCAGGGTA
    TATGATAATT TCAAGACTGG AACATTTGTG CTTTATAAGA GTATGTCACG TGATTTTGAA
    GTCCATGTAC GTCAGTGGGA CTGCAGAAGC CTTCACTATC CAGTGTCATG TAATTGTGGG
    TTTGTTGCCC AGGAAGGAGG TGATATAGTT ACTTTTGATA TGTGCAATGG TCAGCTACGT
    GAATCACAAC CATATTTGTT CATAAAAAGC CAAGATGTAA CCAGGAATAT CAAGATAAGT
    GAATCTTACT TAGGAAGAAA AGTCACAATC TGGTTTTCTT CTGGGGCATT TATCCGTGCT
    GATCTTGGTG AATGGGGCAT GAGTCTAACG ATCAGAGCCC CTAGTGTAGA TTACAGAAAC
    ACTCTGGGAC TTTGTGGAAC CTTTGATGAA AATCCGGAAA ATGATTTCCA TGACAAAAAT
    GGGATGCAAA TTGATCAAAA TTTTAACAAT TATGTTGCTT TTATTAATGA ATGGAGGATT
    TTACCAGGAA AAAGCATGTC TGACACACTG CCAGTTTCTA TGACATCACC TGGAAAGCCA
    TCCTATTGTA GCTGTTCATT GGACACTGCA GCGTATCCGT CTTCCGAAGA TCTGGACAGT
    GTTTCTCGAT CAGAAATTGC CTTGGGTTGC AAAGACCTCA ATCATGTCAG CTTATCTTCT
    TTGATACCAG AACTAGATGT CACCTCCGAA TATATTAATT CAGACACTCT TGTCAGAGAG
    ATAAACAAGC ATACATCTCC AGAAGAATAT AATCTAAATT TATTTCTGCA AGAAAAAAAA
    CACATAAACC TGACTAAACT CGGCTTAAAT GTACAAAAAC ATCCTGGAAA TGAGAAAGAA
    GATTCACTAC AATATTTGGC CAATAAGAAA TATACACAAG GCCGGGGAAG CCACAGCCAA
    GAAATGAGGT ACAATCGACA AAACAGATGG AAACGGCAGA ACTTTCATGA GTTTCCTCCT
    TTGTTTGCTT TCCCGAGTCT CAGCCAAACG GATCTGGAAG AACTTACTTA TTTTTTCCCA
    GAGGACCATG CTGAGGATGT ACAGCAAGAG TTTTTCCCCT CTTGGCCCAC TCCCTCTGGC
    CTCACCGAGT ATAGCACCTT GACCCTCTGT CAGGAGACTC TAGCCAACTC CAGCATAGGA
    AGGCTGTGTC TTGCTTTTCT TGGCAAGAGA TTAGACAGTG TTATAGAGAT GTGTGTGAAG
    GATGTTCTGT TAAAAGATGA TCTTAGTTGG GCAGAAGCAG GTGTGGCCCT TTTAGAAAAT
    GAATGTGAAA AGAGGATTGT GGAGGAGGGG AAATATAACA CAGAAGAGTA TGGCACATCA
    ATTGAAGACA TTCTCTCAGT ATTAAAATGC CCCAATTTAT GCAGCGGCAA TGGGCAGTGC
    ATGGAATGGG GGTGTGCGTG TTCCCCAAGC TTTAGTTCCT ATGACTGCAG TGATTCCTAT
    GACAAAGCTC CTGAAATTAC AGAGCTTGGG AATGCTGGAT TCTGTGATGT TCAAAAATAT
    AATTGTATGA TGGTGAGAGT TTTTGGCAAA GGCTTCAAAG AATTACCTTC AATTAAATGT
    GAAGTTACTA AGCTACAGTA TAATAGCAGT GAATGGATGC CTGGAGAACC CATCTATACA
    CAGACTGTTT TCCACAATAG CAGAGCTGTT GATTGTCAGC TGCCCACTGA TGTTCAGCAG
    TTTGATACCA TGGATCTGGT GGGTGGGAAA CCAACTGGGA AGTGGCAGTT GAAGGTATCT
    AATGATGGTT ATAAATTCAG TAATCCCAAA ATAACGGTCA TATATGATGG TGCTTGCCAA
    GTTTGTGGTC TCTATAAAAA TGACTCATGT ACTATAAAGG AAAATGTTTG CATTATTGAT
    GGACTCTGCT ATGTTGAAGG AGACAAAAAT CCAACCAGCC CTTGTTTGAT TTGTAGACCC
    AAAATTTCAA GATTTACTTG GTCATTTTTA GAAAACAACC AGCCCCCAGT GATTCAAGCA
    TTGCAAGACA AATTACAGAC ATTTTATGGT GAAAACTTTG AGTATCAGTT CGTGGCCTTC
    GATCCAGAAG GTTCTGACAT CCATTTTACG TTGGACTCTG GTCCTGAAGG GGCAAGTGTT
    TCCTCTGCAG GGCTTTTTAT GTGGAAAACA GATTTACTAA CTACCCAACA AATTACTGTA
    CGCCTTAATG ATGACTGCGA TGCTGAAACT AGAGTCACGA TTGAGGTGAC TGTGAAGTCT
    TGTGATTGCT TGAATGGTGG ATCATGTGTA TCTGATAGGA ACTTTTCTCC AGGGAGTGGA
    GTGTACCTGT GTGTCTGCTT GCCTGGCTTC CATGGCAGCC TTTGTGAAGT GGACATTAGT
    GGGTGCCAAT CCAACCCTTG TGGTCTTGGC AGCTATATTA GTGGTTTTCA CAGTTATTCC
    TGTGATTGTC CACCTGAGCT CAAAGTTGAA ACACAGTTTG TAAATCAATT TACTACACAA
    ACTGTGGTTC TCACAAGATC TGATAAAAGT GTAAATAAAG AAGAGGATGA TAAAAATGCC
    CAAGGGAGAA AGAGGCATGT TAAGCCAACA AGTGGAAATG CCTTCACCAT TTGTAAATAT
    CCATGTGGAA AAAGTAGGGA GTGTGTTGCC CCAAACATCT GCAAATGTAA ACCTGGTTAC
    ATTGGTTCTA ACTGCCAAAC TGCTCTTTGT GACCCTGATT GCAAAAACCA TGGAAAATGT
    ATTAAGCCTA ACATTTGTCA GTGTCTTCCA GGACATGGTG GAGCAACCTG TGATGAAGAA
    CATTGTAACC CACCTTGTCA ACATGGTGGC ACATGCTTGG CTGGGAATCT CTGCACTTGT
    CCTTATGGTT TTGTAGGACC AAGGTGTGAA ACAATGGTTT GTAACAGGCA CTGTGAAAAT
    GGAGGCCAGT GTCTTACACC AGATATTTGC CAGTGCAAAC CTGGCTGGTA TGGACCCACC
    TGTAGTACAG CTTTGTGCGA CCCTGTCTGC CTCAATGGTG GTTCGTGTAA TAAGCCAAAT
    ACTTGCCTCT GTCCAAATGG ATTCTTTGGG GAACACTGTC AGAATGCTTT CTGTCACCCT
    CCCTGTAAGA ATGGTGGCCA CTGCATGAGA AATAATGTGT GCGTCTGTCG TGAAGGATAC
    ACTGGTAGGA GATTCCAAAA AAGCATCTGT GATCCTACGT GCATGAATGG AGGAAAATGT
    GTGGGACCAA GCACTTGCTC TTGTCCTTCT GGTTGGAGTG GGAAACGATG CAACACACCT
    ATCTGCTTGC AGAAATGTAA AAACGGTGGT GAATGCATTG CGCCCAGCAT ATGCCATTGT
    CCTTCCTCCT GGGAAGGAGT GCGGTGTCAA ATACCAATTT GCAACCCAAA ATGTCTTTAT
    GGAGGCAGAT GCATATTTCC CAATGTGTGT TCCTGCCGCA CTGAATACTC TGGAGTCAAA
    TGTGAGAAGA AAATACAGAT AAGACGCCAT TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu102142
    Clone ID Related Accession (Same CDS sequence) NM_001346972.1
    Accession Version NM_001346972.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4428bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-02-25
    Organism Homo sapiens(human)
    Product von Willebrand factor D and EGF domain-containing protein isoform 2 precursor
    Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC013470.10 and AC007321.2. On Nov 12, 2016 this sequence version replaced XM_017011828.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## Transcript exon combination :: SRR1660807.106951.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    ATGCCTGGCG GAGCCTGCGT GCTGGTGATC GCGCTGATGT TCCTGGCCTG GGGGGAAGCT 
    CAGGAGTGCT CTCCTGGGGG ACACCAGTTT CTTCGGAGTC CTTATAGAAG TGTCCGTTTT
    GACTCATGGC ACCTCCAGCA GTCAGCTGTT CAAGACCTAA TATGTGACCA TTCCCTCTCC
    CCTGGATGGT ATAGATTTCT CATCCTTGAC AGACCTGCCG AGATGCCAAC CAAATGTGTT
    GAGATGAACC ATTGTGGAAC TCAGGCCCCC ATCTGGCTGT CTCTGAGAGA TTCAGAAACA
    CTGCCATCTC CTGGGGAGAT CAAGCAATTG ACAGCTTGTG CAACATGGCA GTTTTTGTTC
    AGCACTACAA AAGACTGCTG TCTCTTTCAA ATCCCAGTGT CTGTAAGAAA CTGTGGGAAC
    TTTTCTGTAT ACTTACTACA ACCAACTCAG GGATGTATGG GCTACTGTGC GGAAGCTATT
    TCTGATGCAC GATTACACCC ATGTGGTTCT GATGAAACTG AAACAGGAGG TGATTGTGTT
    CGTCAGCTGG CTGCCTCATT GCCACCTCCA CCTGCAGGAA GGCCAGAGGT TCTGGTGGAG
    TTGATTGAGT CCAGGCTTTT CTGTAGGTGT TCTTTTGATG TTCCCGCTAC AAAAAACTCA
    GTGGGATTTC ACATAGCTTG GTCTAGGCTT TCTTCTCAAG AAGTCAAAGA GGAGCTGACA
    CAAGAGACCA CAGTTCAGGC ATTCTCTCTT TTAGAACTTG ATGGCATAAA TCTCAGACTT
    GGAGACAGGA TATTCTGCAG CGCTTCTGTC TTTTTCTTGG AGAATCCTCA TGTACAAAGT
    GTAGCCATCG AAAGCCAAGA ATTTTTTGCA GGCTTTAAGC TACAGCCTGA ATTGAGCACT
    ATATCAGAGG ATGGGAAAGA ATACTACCTG AGGATAGAAA GCACAGTTCC TATTATTTGT
    TCTGAATTTA GTGAGCTTGA TCAAGAATGC AAAATCTCAT TAAAACTGAA AACTATTGGT
    CAAGGTAGAG AGCACCTAGG CTTAAATTTG GCACTGTCTT CCTGTCATGT GGACCTTCTC
    CAGACATCTT CCTGTGCTAA TGGAACCTGT AGCCACACTT TTGTGTACTA CACTGCTGTC
    ACAGATTTTT CTCGAGATGG AGATAGAGTC TCAAACATTG TAGTGCAACC AATAGTTAAT
    GAGGATTTCC TGTGGAACAA CTACATTCCA GACAGCATCC AGATCTGGTT TTCTTCTGGG
    GCATTTATCC GTGCTGATCT TGGTGAATGG GGCATGAGTC TAACGATCAG AGCCCCTAGT
    GTAGATTACA GAAACACTCT GGGACTTTGT GGAACCTTTG ATGAAAATCC GGAAAATGAT
    TTCCATGACA AAAATGGGAT GCAAATTGAT CAAAATTTTA ACAATTATGT TGCTTTTATT
    AATGAATGGA GGATTTTACC AGGAAAAAGC ATGTCTGACA CACTGCCAGT TTCTATGACA
    TCACCTGGAA AGCCATCCTA TTGTAGCTGT TCATTGGACA CTGCAGCGTA TCCGTCTTCC
    GAAGATCTGG ACAGTGTTTC TCGATCAGAA ATTGCCTTGG GTTGCAAAGA CCTCAATCAT
    GTCAGCTTAT CTTCTTTGAT ACCAGAACTA GATGTCACCT CCGAATATAT TAATTCAGAC
    ACTCTTGTCA GAGAGATAAA CAAGCATACA TCTCCAGAAG AATATAATCT AAATTTATTT
    CTGCAAGAAA AAAAACACAT AAACCTGACT AAACTCGGCT TAAATGTACA AAAACATCCT
    GGAAATGAGA AAGAAGATTC ACTACAATAT TTGGCCAATA AGAAATATAC ACAAGGCCGG
    GGAAGCCACA GCCAAGAAAT GAGGTACAAT CGACAAAACA GATGGAAACG GCAGAACTTT
    CATGAGTTTC CTCCTTTGTT TGCTTTCCCG AGTCTCAGCC AAACGGATCT GGAAGAACTT
    ACTTATTTTT TCCCAGAGGA CCATGCTGAG GATGTACAGC AAGAGTTTTT CCCCTCTTGG
    CCCACTCCCT CTGGCCTCAC CGAGTATAGC ACCTTGACCC TCTGTCAGGA GACTCTAGCC
    AACTCCAGCA TAGGAAGGCT GTGTCTTGCT TTTCTTGGCA AGAGATTAGA CAGTGTTATA
    GAGATGTGTG TGAAGGATGT TCTGTTAAAA GATGATCTTA GTTGGGCAGA AGCAGGTGTG
    GCCCTTTTAG AAAATGAATG TGAAAAGAGG ATTGTGGAGG AGGGGAAATA TAACACAGAA
    GAGTATGGCA CATCAATTGA AGACATTCTC TCAGTATTAA AATGCCCCAA TTTATGCAGC
    GGCAATGGGC AGTGCATGGA ATGGGGGTGT GCGTGTTCCC CAAGCTTTAG TTCCTATGAC
    TGCAGTGATT CCTATGACAA AGCTCCTGAA ATTACAGAGC TTGGGAATGC TGGATTCTGT
    GATGTTCAAA AATATAATTG TATGATGGTG AGAGTTTTTG GCAAAGGCTT CAAAGAATTA
    CCTTCAATTA AATGTGAAGT TACTAAGCTA CAGTATAATA GCAGTGAATG GATGCCTGGA
    GAACCCATCT ATACACAGAC TGTTTTCCAC AATAGCAGAG CTGTTGATTG TCAGCTGCCC
    ACTGATGTTC AGCAGTTTGA TACCATGGAT CTGGTGGGTG GGAAACCAAC TGGGAAGTGG
    CAGTTGAAGG TATCTAATGA TGGTTATAAA TTCAGTAATC CCAAAATAAC GGTCATATAT
    GATGGTGCTT GCCAAGTTTG TGGTCTCTAT AAAAATGACT CATGTACTAT AAAGGAAAAT
    GTTTGCATTA TTGATGGACT CTGCTATGTT GAAGGAGACA AAAATCCAAC CAGCCCTTGT
    TTGATTTGTA GACCCAAAAT TTCAAGATTT ACTTGGTCAT TTTTAGAAAA CAACCAGCCC
    CCAGTGATTC AAGCATTGCA AGACAAATTA CAGACATTTT ATGGTGAAAA CTTTGAGTAT
    CAGTTCGTGG CCTTCGATCC AGAAGGTTCT GACATCCATT TTACGTTGGA CTCTGGTCCT
    GAAGGGGCAA GTGTTTCCTC TGCAGGGCTT TTTATGTGGA AAACAGATTT ACTAACTACC
    CAACAAATTA CTGTACGCCT TAATGATGAC TGCGATGCTG AAACTAGAGT CACGATTGAG
    GTGACTGTGA AGTCTTGTGA TTGCTTGAAT GGTGGATCAT GTGTATCTGA TAGGAACTTT
    TCTCCAGGGA GTGGAGTGTA CCTGTGTGTC TGCTTGCCTG GCTTCCATGG CAGCCTTTGT
    GAAGTGGACA TTAGTGGGTG CCAATCCAAC CCTTGTGGTC TTGGCAGCTA TATTAGTGGT
    TTTCACAGTT ATTCCTGTGA TTGTCCACCT GAGCTCAAAG TTGAAACACA GTTTGTAAAT
    CAATTTACTA CACAAACTGT GGTTCTCACA AGATCTGATA AAAGTGTAAA TAAAGAAGAG
    GATGATAAAA ATGCCCAAGG GAGAAAGAGG CATGTTAAGC CAACAAGTGG AAATGCCTTC
    ACCATTTGTA AATATCCATG TGGAAAAAGT AGGGAGTGTG TTGCCCCAAA CATCTGCAAA
    TGTAAACCTG GTTACATTGG TTCTAACTGC CAAACTGCTC TTTGTGACCC TGATTGCAAA
    AACCATGGAA AATGTATTAA GCCTAACATT TGTCAGTGTC TTCCAGGACA TGGTGGAGCA
    ACCTGTGATG AAGAACATTG TAACCCACCT TGTCAACATG GTGGCACATG CTTGGCTGGG
    AATCTCTGCA CTTGTCCTTA TGGTTTTGTA GGACCAAGGT GTGAAACAAT GGTTTGTAAC
    AGGCACTGTG AAAATGGAGG CCAGTGTCTT ACACCAGATA TTTGCCAGTG CAAACCTGGC
    TGGTATGGAC CCACCTGTAG TACAGCTTTG TGCGACCCTG TCTGCCTCAA TGGTGGTTCG
    TGTAATAAGC CAAATACTTG CCTCTGTCCA AATGGATTCT TTGGGGAACA CTGTCAGAAT
    GCTTTCTGTC ACCCTCCCTG TAAGAATGGT GGCCACTGCA TGAGAAATAA TGTGTGCGTC
    TGTCGTGAAG GATACACTGG TAGGAGATTC CAAAAAAGCA TCTGTGATCC TACGTGCATG
    AATGGAGGAA AATGTGTGGG ACCAAGCACT TGCTCTTGTC CTTCTGGTTG GAGTGGGAAA
    CGATGCAACA CACCTATCTG CTTGCAGAAA TGTAAAAACG GTGGTGAATG CATTGCGCCC
    AGCATATGCC ATTGTCCTTC CTCCTGGGAA GGAGTGCGGT GTCAAATACC AATTTGCAAC
    CCAAAATGTC TTTATGGAGG CAGATGCATA TTTCCCAATG TGTGTTCCTG CCGCACTGAA
    TACTCTGGAG TCAAATGTGA GAAGAAAATA CAGATAAGAC GCCATTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001333901.1
    CDS226..4653
    Translation

    Target ORF information:

    RefSeq Version NM_001346972.1
    Organism Homo sapiens(human)
    Definition Homo sapiens von Willebrand factor D and EGF domains (VWDE), transcript variant 2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001346972.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    ATGCCTGGCG GAGCCTGCGT GCTGGTGATC GCGCTGATGT TCCTGGCCTG GGGGGAAGCT 
    CAGGAGTGCT CTCCTGGGGG ACACCAGTTT CTTCGGAGTC CTTATAGAAG TGTCCGTTTT
    GACTCATGGC ACCTCCAGCA GTCAGCTGTT CAAGACCTAA TATGTGACCA TTCCCTCTCC
    CCTGGATGGT ATAGATTTCT CATCCTTGAC AGACCTGCCG AGATGCCAAC CAAATGTGTT
    GAGATGAACC ATTGTGGAAC TCAGGCCCCC ATCTGGCTGT CTCTGAGAGA TTCAGAAACA
    CTGCCATCTC CTGGGGAGAT CAAGCAATTG ACAGCTTGTG CAACATGGCA GTTTTTGTTC
    AGCACTACAA AAGACTGCTG TCTCTTTCAA ATCCCAGTGT CTGTAAGAAA CTGTGGGAAC
    TTTTCTGTAT ACTTACTACA ACCAACTCAG GGATGTATGG GCTACTGTGC GGAAGCTATT
    TCTGATGCAC GATTACACCC ATGTGGTTCT GATGAAACTG AAACAGGAGG TGATTGTGTT
    CGTCAGCTGG CTGCCTCATT GCCACCTCCA CCTGCAGGAA GGCCAGAGGT TCTGGTGGAG
    TTGATTGAGT CCAGGCTTTT CTGTAGGTGT TCTTTTGATG TTCCCGCTAC AAAAAACTCA
    GTGGGATTTC ACATAGCTTG GTCTAGGCTT TCTTCTCAAG AAGTCAAAGA GGAGCTGACA
    CAAGAGACCA CAGTTCAGGC ATTCTCTCTT TTAGAACTTG ATGGCATAAA TCTCAGACTT
    GGAGACAGGA TATTCTGCAG CGCTTCTGTC TTTTTCTTGG AGAATCCTCA TGTACAAAGT
    GTAGCCATCG AAAGCCAAGA ATTTTTTGCA GGCTTTAAGC TACAGCCTGA ATTGAGCACT
    ATATCAGAGG ATGGGAAAGA ATACTACCTG AGGATAGAAA GCACAGTTCC TATTATTTGT
    TCTGAATTTA GTGAGCTTGA TCAAGAATGC AAAATCTCAT TAAAACTGAA AACTATTGGT
    CAAGGTAGAG AGCACCTAGG CTTAAATTTG GCACTGTCTT CCTGTCATGT GGACCTTCTC
    CAGACATCTT CCTGTGCTAA TGGAACCTGT AGCCACACTT TTGTGTACTA CACTGCTGTC
    ACAGATTTTT CTCGAGATGG AGATAGAGTC TCAAACATTG TAGTGCAACC AATAGTTAAT
    GAGGATTTCC TGTGGAACAA CTACATTCCA GACAGCATCC AGATCTGGTT TTCTTCTGGG
    GCATTTATCC GTGCTGATCT TGGTGAATGG GGCATGAGTC TAACGATCAG AGCCCCTAGT
    GTAGATTACA GAAACACTCT GGGACTTTGT GGAACCTTTG ATGAAAATCC GGAAAATGAT
    TTCCATGACA AAAATGGGAT GCAAATTGAT CAAAATTTTA ACAATTATGT TGCTTTTATT
    AATGAATGGA GGATTTTACC AGGAAAAAGC ATGTCTGACA CACTGCCAGT TTCTATGACA
    TCACCTGGAA AGCCATCCTA TTGTAGCTGT TCATTGGACA CTGCAGCGTA TCCGTCTTCC
    GAAGATCTGG ACAGTGTTTC TCGATCAGAA ATTGCCTTGG GTTGCAAAGA CCTCAATCAT
    GTCAGCTTAT CTTCTTTGAT ACCAGAACTA GATGTCACCT CCGAATATAT TAATTCAGAC
    ACTCTTGTCA GAGAGATAAA CAAGCATACA TCTCCAGAAG AATATAATCT AAATTTATTT
    CTGCAAGAAA AAAAACACAT AAACCTGACT AAACTCGGCT TAAATGTACA AAAACATCCT
    GGAAATGAGA AAGAAGATTC ACTACAATAT TTGGCCAATA AGAAATATAC ACAAGGCCGG
    GGAAGCCACA GCCAAGAAAT GAGGTACAAT CGACAAAACA GATGGAAACG GCAGAACTTT
    CATGAGTTTC CTCCTTTGTT TGCTTTCCCG AGTCTCAGCC AAACGGATCT GGAAGAACTT
    ACTTATTTTT TCCCAGAGGA CCATGCTGAG GATGTACAGC AAGAGTTTTT CCCCTCTTGG
    CCCACTCCCT CTGGCCTCAC CGAGTATAGC ACCTTGACCC TCTGTCAGGA GACTCTAGCC
    AACTCCAGCA TAGGAAGGCT GTGTCTTGCT TTTCTTGGCA AGAGATTAGA CAGTGTTATA
    GAGATGTGTG TGAAGGATGT TCTGTTAAAA GATGATCTTA GTTGGGCAGA AGCAGGTGTG
    GCCCTTTTAG AAAATGAATG TGAAAAGAGG ATTGTGGAGG AGGGGAAATA TAACACAGAA
    GAGTATGGCA CATCAATTGA AGACATTCTC TCAGTATTAA AATGCCCCAA TTTATGCAGC
    GGCAATGGGC AGTGCATGGA ATGGGGGTGT GCGTGTTCCC CAAGCTTTAG TTCCTATGAC
    TGCAGTGATT CCTATGACAA AGCTCCTGAA ATTACAGAGC TTGGGAATGC TGGATTCTGT
    GATGTTCAAA AATATAATTG TATGATGGTG AGAGTTTTTG GCAAAGGCTT CAAAGAATTA
    CCTTCAATTA AATGTGAAGT TACTAAGCTA CAGTATAATA GCAGTGAATG GATGCCTGGA
    GAACCCATCT ATACACAGAC TGTTTTCCAC AATAGCAGAG CTGTTGATTG TCAGCTGCCC
    ACTGATGTTC AGCAGTTTGA TACCATGGAT CTGGTGGGTG GGAAACCAAC TGGGAAGTGG
    CAGTTGAAGG TATCTAATGA TGGTTATAAA TTCAGTAATC CCAAAATAAC GGTCATATAT
    GATGGTGCTT GCCAAGTTTG TGGTCTCTAT AAAAATGACT CATGTACTAT AAAGGAAAAT
    GTTTGCATTA TTGATGGACT CTGCTATGTT GAAGGAGACA AAAATCCAAC CAGCCCTTGT
    TTGATTTGTA GACCCAAAAT TTCAAGATTT ACTTGGTCAT TTTTAGAAAA CAACCAGCCC
    CCAGTGATTC AAGCATTGCA AGACAAATTA CAGACATTTT ATGGTGAAAA CTTTGAGTAT
    CAGTTCGTGG CCTTCGATCC AGAAGGTTCT GACATCCATT TTACGTTGGA CTCTGGTCCT
    GAAGGGGCAA GTGTTTCCTC TGCAGGGCTT TTTATGTGGA AAACAGATTT ACTAACTACC
    CAACAAATTA CTGTACGCCT TAATGATGAC TGCGATGCTG AAACTAGAGT CACGATTGAG
    GTGACTGTGA AGTCTTGTGA TTGCTTGAAT GGTGGATCAT GTGTATCTGA TAGGAACTTT
    TCTCCAGGGA GTGGAGTGTA CCTGTGTGTC TGCTTGCCTG GCTTCCATGG CAGCCTTTGT
    GAAGTGGACA TTAGTGGGTG CCAATCCAAC CCTTGTGGTC TTGGCAGCTA TATTAGTGGT
    TTTCACAGTT ATTCCTGTGA TTGTCCACCT GAGCTCAAAG TTGAAACACA GTTTGTAAAT
    CAATTTACTA CACAAACTGT GGTTCTCACA AGATCTGATA AAAGTGTAAA TAAAGAAGAG
    GATGATAAAA ATGCCCAAGG GAGAAAGAGG CATGTTAAGC CAACAAGTGG AAATGCCTTC
    ACCATTTGTA AATATCCATG TGGAAAAAGT AGGGAGTGTG TTGCCCCAAA CATCTGCAAA
    TGTAAACCTG GTTACATTGG TTCTAACTGC CAAACTGCTC TTTGTGACCC TGATTGCAAA
    AACCATGGAA AATGTATTAA GCCTAACATT TGTCAGTGTC TTCCAGGACA TGGTGGAGCA
    ACCTGTGATG AAGAACATTG TAACCCACCT TGTCAACATG GTGGCACATG CTTGGCTGGG
    AATCTCTGCA CTTGTCCTTA TGGTTTTGTA GGACCAAGGT GTGAAACAAT GGTTTGTAAC
    AGGCACTGTG AAAATGGAGG CCAGTGTCTT ACACCAGATA TTTGCCAGTG CAAACCTGGC
    TGGTATGGAC CCACCTGTAG TACAGCTTTG TGCGACCCTG TCTGCCTCAA TGGTGGTTCG
    TGTAATAAGC CAAATACTTG CCTCTGTCCA AATGGATTCT TTGGGGAACA CTGTCAGAAT
    GCTTTCTGTC ACCCTCCCTG TAAGAATGGT GGCCACTGCA TGAGAAATAA TGTGTGCGTC
    TGTCGTGAAG GATACACTGG TAGGAGATTC CAAAAAAGCA TCTGTGATCC TACGTGCATG
    AATGGAGGAA AATGTGTGGG ACCAAGCACT TGCTCTTGTC CTTCTGGTTG GAGTGGGAAA
    CGATGCAACA CACCTATCTG CTTGCAGAAA TGTAAAAACG GTGGTGAATG CATTGCGCCC
    AGCATATGCC ATTGTCCTTC CTCCTGGGAA GGAGTGCGGT GTCAAATACC AATTTGCAAC
    CCAAAATGTC TTTATGGAGG CAGATGCATA TTTCCCAATG TGTGTTCCTG CCGCACTGAA
    TACTCTGGAG TCAAATGTGA GAAGAAAATA CAGATAAGAC GCCATTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu97082
    Clone ID Related Accession (Same CDS sequence) NM_001346973.1 , XM_017011829.1
    Accession Version NM_001346973.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3963bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-06-30
    Organism Homo sapiens(human)
    Product von Willebrand factor D and EGF domain-containing protein isoform 3
    Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC013470.10 and AC007321.2. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## CDS exon combination :: SRR1660805.42324.1 [ECO:0000331] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    ATGAAGAAGG CTATTTCTGA TGCACGATTA CACCCATGTG GTTCTGATGA AACTGAAACA 
    GGAGGTGATT GTGTTCGTCA GCTGGCTGCC TCATTGCCAC CTCCACCTGC AGGAAGGCCA
    GAGGTTCTGG TGGAGTTGAT TGAGTCCAGG CTTTTCTGTA GGTGTTCTTT TGATGTTCCC
    GCTACAAAAA ACTCAGTGGG ATTTCACATA GCTTGGTCTA GGCTTTCTTC TCAAGAAGTC
    AAAGAGGAGC TGACACAAGA GACCACAGTT CAGGCATTCT CTCTTTTAGA ACTTGATGGC
    ATAAATCTCA GACTTGGAGA CAGGATATTC TGCAGCGCTT CTGTCTTTTT CTTGGAGAAT
    CCTCATGTAC AAAGTGTAGC CATCGAAAGC CAAGAATTTT TTGCAGGCTT TAAGCTACAG
    CCTGAATTGA GCACTATATC AGAGGATGGG AAAGAATACT ACCTGAGGAT AGAAAGCACA
    GTTCCTATTA TTTGTTCTGA ATTTAGTGAG CTTGATCAAG AATGCAAAAT CTCATTAAAA
    CTGAAAACTA TTGGTCAAGG TAGAGAGCAC CTAGGCTTAA ATTTGGCACT GTCTTCCTGT
    CATGTGGACC TTCTCCAGAC ATCTTCCTGT GCTAATGGAA CCTGTAGCCA CACTTTTGTG
    TACTACACTG CTGTCACAGA TTTTTCTCGA GATGGAGATA GAGTCTCAAA CATTGTAGTG
    CAACCAATAG TTAATGAGGA TTTCCTGTGG AACAACTACA TTCCAGACAG CATCCAGATC
    TGGTTTTCTT CTGGGGCATT TATCCGTGCT GATCTTGGTG AATGGGGCAT GAGTCTAACG
    ATCAGAGCCC CTAGTGTAGA TTACAGAAAC ACTCTGGGAC TTTGTGGAAC CTTTGATGAA
    AATCCGGAAA ATGATTTCCA TGACAAAAAT GGGATGCAAA TTGATCAAAA TTTTAACAAT
    TATGTTGCTT TTATTAATGA ATGGAGGATT TTACCAGGAA AAAGCATGTC TGACACACTG
    CCAGTTTCTA TGACATCACC TGGAAAGCCA TCCTATTGTA GCTGTTCATT GGACACTGCA
    GCGTATCCGT CTTCCGAAGA TCTGGACAGT GTTTCTCGAT CAGAAATTGC CTTGGGTTGC
    AAAGACCTCA ATCATGTCAG CTTATCTTCT TTGATACCAG AACTAGATGT CACCTCCGAA
    TATATTAATT CAGACACTCT TGTCAGAGAG ATAAACAAGC ATACATCTCC AGAAGAATAT
    AATCTAAATT TATTTCTGCA AGAAAAAAAA CACATAAACC TGACTAAACT CGGCTTAAAT
    GTACAAAAAC ATCCTGGAAA TGAGAAAGAA GATTCACTAC AATATTTGGC CAATAAGAAA
    TATACACAAG GCCGGGGAAG CCACAGCCAA GAAATGAGGT ACAATCGACA AAACAGATGG
    AAACGGCAGA ACTTTCATGA GTTTCCTCCT TTGTTTGCTT TCCCGAGTCT CAGCCAAACG
    GATCTGGAAG AACTTACTTA TTTTTTCCCA GAGGACCATG CTGAGGATGT ACAGCAAGAG
    TTTTTCCCCT CTTGGCCCAC TCCCTCTGGC CTCACCGAGT ATAGCACCTT GACCCTCTGT
    CAGGAGACTC TAGCCAACTC CAGCATAGGA AGGCTGTGTC TTGCTTTTCT TGGCAAGAGA
    TTAGACAGTG TTATAGAGAT GTGTGTGAAG GATGTTCTGT TAAAAGATGA TCTTAGTTGG
    GCAGAAGCAG GTGTGGCCCT TTTAGAAAAT GAATGTGAAA AGAGGATTGT GGAGGAGGGG
    AAATATAACA CAGAAGAGTA TGGCACATCA ATTGAAGACA TTCTCTCAGT ATTAAAATGC
    CCCAATTTAT GCAGCGGCAA TGGGCAGTGC ATGGAATGGG GGTGTGCGTG TTCCCCAAGC
    TTTAGTTCCT ATGACTGCAG TGATTCCTAT GACAAAGCTC CTGAAATTAC AGAGCTTGGG
    AATGCTGGAT TCTGTGATGT TCAAAAATAT AATTGTATGA TGGTGAGAGT TTTTGGCAAA
    GGCTTCAAAG AATTACCTTC AATTAAATGT GAAGTTACTA AGCTACAGTA TAATAGCAGT
    GAATGGATGC CTGGAGAACC CATCTATACA CAGACTGTTT TCCACAATAG CAGAGCTGTT
    GATTGTCAGC TGCCCACTGA TGTTCAGCAG TTTGATACCA TGGATCTGGT GGGTGGGAAA
    CCAACTGGGA AGTGGCAGTT GAAGGTATCT AATGATGGTT ATAAATTCAG TAATCCCAAA
    ATAACGGTCA TATATGATGG TGCTTGCCAA GTTTGTGGTC TCTATAAAAA TGACTCATGT
    ACTATAAAGG AAAATGTTTG CATTATTGAT GGACTCTGCT ATGTTGAAGG AGACAAAAAT
    CCAACCAGCC CTTGTTTGAT TTGTAGACCC AAAATTTCAA GATTTACTTG GTCATTTTTA
    GAAAACAACC AGCCCCCAGT GATTCAAGCA TTGCAAGACA AATTACAGAC ATTTTATGGT
    GAAAACTTTG AGTATCAGTT CGTGGCCTTC GATCCAGAAG GTTCTGACAT CCATTTTACG
    TTGGACTCTG GTCCTGAAGG GGCAAGTGTT TCCTCTGCAG GGCTTTTTAT GTGGAAAACA
    GATTTACTAA CTACCCAACA AATTACTGTA CGCCTTAATG ATGACTGCGA TGCTGAAACT
    AGAGTCACGA TTGAGGTGAC TGTGAAGTCT TGTGATTGCT TGAATGGTGG ATCATGTGTA
    TCTGATAGGA ACTTTTCTCC AGGGAGTGGA GTGTACCTGT GTGTCTGCTT GCCTGGCTTC
    CATGGCAGCC TTTGTGAAGT GGACATTAGT GGGTGCCAAT CCAACCCTTG TGGTCTTGGC
    AGCTATATTA GTGGTTTTCA CAGTTATTCC TGTGATTGTC CACCTGAGCT CAAAGTTGAA
    ACACAGTTTG TAAATCAATT TACTACACAA ACTGTGGTTC TCACAAGATC TGATAAAAGT
    GTAAATAAAG AAGAGGATGA TAAAAATGCC CAAGGGAGAA AGAGGCATGT TAAGCCAACA
    AGTGGAAATG CCTTCACCAT TTGTAAATAT CCATGTGGAA AAAGTAGGGA GTGTGTTGCC
    CCAAACATCT GCAAATGTAA ACCTGGTTAC ATTGGTTCTA ACTGCCAAAC TGCTCTTTGT
    GACCCTGATT GCAAAAACCA TGGAAAATGT ATTAAGCCTA ACATTTGTCA GTGTCTTCCA
    GGACATGGTG GAGCAACCTG TGATGAAGAA CATTGTAACC CACCTTGTCA ACATGGTGGC
    ACATGCTTGG CTGGGAATCT CTGCACTTGT CCTTATGGTT TTGTAGGACC AAGGTGTGAA
    ACAATGGTTT GTAACAGGCA CTGTGAAAAT GGAGGCCAGT GTCTTACACC AGATATTTGC
    CAGTGCAAAC CTGGCTGGTA TGGACCCACC TGTAGTACAG CTTTGTGCGA CCCTGTCTGC
    CTCAATGGTG GTTCGTGTAA TAAGCCAAAT ACTTGCCTCT GTCCAAATGG ATTCTTTGGG
    GAACACTGTC AGAATGCTTT CTGTCACCCT CCCTGTAAGA ATGGTGGCCA CTGCATGAGA
    AATAATGTGT GCGTCTGTCG TGAAGGATAC ACTGGTAGGA GATTCCAAAA AAGCATCTGT
    GATCCTACGT GCATGAATGG AGGAAAATGT GTGGGACCAA GCACTTGCTC TTGTCCTTCT
    GGTTGGAGTG GGAAACGATG CAACACACCT ATCTGCTTGC AGAAATGTAA AAACGGTGGT
    GAATGCATTG CGCCCAGCAT ATGCCATTGT CCTTCCTCCT GGGAAGGAGT GCGGTGTCAA
    ATACCAATTT GCAACCCAAA ATGTCTTTAT GGAGGCAGAT GCATATTTCC CAATGTGTGT
    TCCTGCCGCA CTGAATACTC TGGAGTCAAA TGTGAGAAGA AAATACAGAT AAGACGCCAT
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001333902.1
    CDS609..4571
    Translation

    Target ORF information:

    RefSeq Version NM_001346973.1
    Organism Homo sapiens(human)
    Definition Homo sapiens von Willebrand factor D and EGF domains (VWDE), transcript variant 3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001346973.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    ATGAAGAAGG CTATTTCTGA TGCACGATTA CACCCATGTG GTTCTGATGA AACTGAAACA 
    GGAGGTGATT GTGTTCGTCA GCTGGCTGCC TCATTGCCAC CTCCACCTGC AGGAAGGCCA
    GAGGTTCTGG TGGAGTTGAT TGAGTCCAGG CTTTTCTGTA GGTGTTCTTT TGATGTTCCC
    GCTACAAAAA ACTCAGTGGG ATTTCACATA GCTTGGTCTA GGCTTTCTTC TCAAGAAGTC
    AAAGAGGAGC TGACACAAGA GACCACAGTT CAGGCATTCT CTCTTTTAGA ACTTGATGGC
    ATAAATCTCA GACTTGGAGA CAGGATATTC TGCAGCGCTT CTGTCTTTTT CTTGGAGAAT
    CCTCATGTAC AAAGTGTAGC CATCGAAAGC CAAGAATTTT TTGCAGGCTT TAAGCTACAG
    CCTGAATTGA GCACTATATC AGAGGATGGG AAAGAATACT ACCTGAGGAT AGAAAGCACA
    GTTCCTATTA TTTGTTCTGA ATTTAGTGAG CTTGATCAAG AATGCAAAAT CTCATTAAAA
    CTGAAAACTA TTGGTCAAGG TAGAGAGCAC CTAGGCTTAA ATTTGGCACT GTCTTCCTGT
    CATGTGGACC TTCTCCAGAC ATCTTCCTGT GCTAATGGAA CCTGTAGCCA CACTTTTGTG
    TACTACACTG CTGTCACAGA TTTTTCTCGA GATGGAGATA GAGTCTCAAA CATTGTAGTG
    CAACCAATAG TTAATGAGGA TTTCCTGTGG AACAACTACA TTCCAGACAG CATCCAGATC
    TGGTTTTCTT CTGGGGCATT TATCCGTGCT GATCTTGGTG AATGGGGCAT GAGTCTAACG
    ATCAGAGCCC CTAGTGTAGA TTACAGAAAC ACTCTGGGAC TTTGTGGAAC CTTTGATGAA
    AATCCGGAAA ATGATTTCCA TGACAAAAAT GGGATGCAAA TTGATCAAAA TTTTAACAAT
    TATGTTGCTT TTATTAATGA ATGGAGGATT TTACCAGGAA AAAGCATGTC TGACACACTG
    CCAGTTTCTA TGACATCACC TGGAAAGCCA TCCTATTGTA GCTGTTCATT GGACACTGCA
    GCGTATCCGT CTTCCGAAGA TCTGGACAGT GTTTCTCGAT CAGAAATTGC CTTGGGTTGC
    AAAGACCTCA ATCATGTCAG CTTATCTTCT TTGATACCAG AACTAGATGT CACCTCCGAA
    TATATTAATT CAGACACTCT TGTCAGAGAG ATAAACAAGC ATACATCTCC AGAAGAATAT
    AATCTAAATT TATTTCTGCA AGAAAAAAAA CACATAAACC TGACTAAACT CGGCTTAAAT
    GTACAAAAAC ATCCTGGAAA TGAGAAAGAA GATTCACTAC AATATTTGGC CAATAAGAAA
    TATACACAAG GCCGGGGAAG CCACAGCCAA GAAATGAGGT ACAATCGACA AAACAGATGG
    AAACGGCAGA ACTTTCATGA GTTTCCTCCT TTGTTTGCTT TCCCGAGTCT CAGCCAAACG
    GATCTGGAAG AACTTACTTA TTTTTTCCCA GAGGACCATG CTGAGGATGT ACAGCAAGAG
    TTTTTCCCCT CTTGGCCCAC TCCCTCTGGC CTCACCGAGT ATAGCACCTT GACCCTCTGT
    CAGGAGACTC TAGCCAACTC CAGCATAGGA AGGCTGTGTC TTGCTTTTCT TGGCAAGAGA
    TTAGACAGTG TTATAGAGAT GTGTGTGAAG GATGTTCTGT TAAAAGATGA TCTTAGTTGG
    GCAGAAGCAG GTGTGGCCCT TTTAGAAAAT GAATGTGAAA AGAGGATTGT GGAGGAGGGG
    AAATATAACA CAGAAGAGTA TGGCACATCA ATTGAAGACA TTCTCTCAGT ATTAAAATGC
    CCCAATTTAT GCAGCGGCAA TGGGCAGTGC ATGGAATGGG GGTGTGCGTG TTCCCCAAGC
    TTTAGTTCCT ATGACTGCAG TGATTCCTAT GACAAAGCTC CTGAAATTAC AGAGCTTGGG
    AATGCTGGAT TCTGTGATGT TCAAAAATAT AATTGTATGA TGGTGAGAGT TTTTGGCAAA
    GGCTTCAAAG AATTACCTTC AATTAAATGT GAAGTTACTA AGCTACAGTA TAATAGCAGT
    GAATGGATGC CTGGAGAACC CATCTATACA CAGACTGTTT TCCACAATAG CAGAGCTGTT
    GATTGTCAGC TGCCCACTGA TGTTCAGCAG TTTGATACCA TGGATCTGGT GGGTGGGAAA
    CCAACTGGGA AGTGGCAGTT GAAGGTATCT AATGATGGTT ATAAATTCAG TAATCCCAAA
    ATAACGGTCA TATATGATGG TGCTTGCCAA GTTTGTGGTC TCTATAAAAA TGACTCATGT
    ACTATAAAGG AAAATGTTTG CATTATTGAT GGACTCTGCT ATGTTGAAGG AGACAAAAAT
    CCAACCAGCC CTTGTTTGAT TTGTAGACCC AAAATTTCAA GATTTACTTG GTCATTTTTA
    GAAAACAACC AGCCCCCAGT GATTCAAGCA TTGCAAGACA AATTACAGAC ATTTTATGGT
    GAAAACTTTG AGTATCAGTT CGTGGCCTTC GATCCAGAAG GTTCTGACAT CCATTTTACG
    TTGGACTCTG GTCCTGAAGG GGCAAGTGTT TCCTCTGCAG GGCTTTTTAT GTGGAAAACA
    GATTTACTAA CTACCCAACA AATTACTGTA CGCCTTAATG ATGACTGCGA TGCTGAAACT
    AGAGTCACGA TTGAGGTGAC TGTGAAGTCT TGTGATTGCT TGAATGGTGG ATCATGTGTA
    TCTGATAGGA ACTTTTCTCC AGGGAGTGGA GTGTACCTGT GTGTCTGCTT GCCTGGCTTC
    CATGGCAGCC TTTGTGAAGT GGACATTAGT GGGTGCCAAT CCAACCCTTG TGGTCTTGGC
    AGCTATATTA GTGGTTTTCA CAGTTATTCC TGTGATTGTC CACCTGAGCT CAAAGTTGAA
    ACACAGTTTG TAAATCAATT TACTACACAA ACTGTGGTTC TCACAAGATC TGATAAAAGT
    GTAAATAAAG AAGAGGATGA TAAAAATGCC CAAGGGAGAA AGAGGCATGT TAAGCCAACA
    AGTGGAAATG CCTTCACCAT TTGTAAATAT CCATGTGGAA AAAGTAGGGA GTGTGTTGCC
    CCAAACATCT GCAAATGTAA ACCTGGTTAC ATTGGTTCTA ACTGCCAAAC TGCTCTTTGT
    GACCCTGATT GCAAAAACCA TGGAAAATGT ATTAAGCCTA ACATTTGTCA GTGTCTTCCA
    GGACATGGTG GAGCAACCTG TGATGAAGAA CATTGTAACC CACCTTGTCA ACATGGTGGC
    ACATGCTTGG CTGGGAATCT CTGCACTTGT CCTTATGGTT TTGTAGGACC AAGGTGTGAA
    ACAATGGTTT GTAACAGGCA CTGTGAAAAT GGAGGCCAGT GTCTTACACC AGATATTTGC
    CAGTGCAAAC CTGGCTGGTA TGGACCCACC TGTAGTACAG CTTTGTGCGA CCCTGTCTGC
    CTCAATGGTG GTTCGTGTAA TAAGCCAAAT ACTTGCCTCT GTCCAAATGG ATTCTTTGGG
    GAACACTGTC AGAATGCTTT CTGTCACCCT CCCTGTAAGA ATGGTGGCCA CTGCATGAGA
    AATAATGTGT GCGTCTGTCG TGAAGGATAC ACTGGTAGGA GATTCCAAAA AAGCATCTGT
    GATCCTACGT GCATGAATGG AGGAAAATGT GTGGGACCAA GCACTTGCTC TTGTCCTTCT
    GGTTGGAGTG GGAAACGATG CAACACACCT ATCTGCTTGC AGAAATGTAA AAACGGTGGT
    GAATGCATTG CGCCCAGCAT ATGCCATTGT CCTTCCTCCT GGGAAGGAGT GCGGTGTCAA
    ATACCAATTT GCAACCCAAA ATGTCTTTAT GGAGGCAGAT GCATATTTCC CAATGTGTGT
    TCCTGCCGCA CTGAATACTC TGGAGTCAAA TGTGAGAAGA AAATACAGAT AAGACGCCAT
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu97082
    Clone ID Related Accession (Same CDS sequence) NM_001346973.1 , XM_017011829.1
    Accession Version XM_017011829.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3963bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product von Willebrand factor D and EGF domain-containing protein isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000007.14) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    ATGAAGAAGG CTATTTCTGA TGCACGATTA CACCCATGTG GTTCTGATGA AACTGAAACA 
    GGAGGTGATT GTGTTCGTCA GCTGGCTGCC TCATTGCCAC CTCCACCTGC AGGAAGGCCA
    GAGGTTCTGG TGGAGTTGAT TGAGTCCAGG CTTTTCTGTA GGTGTTCTTT TGATGTTCCC
    GCTACAAAAA ACTCAGTGGG ATTTCACATA GCTTGGTCTA GGCTTTCTTC TCAAGAAGTC
    AAAGAGGAGC TGACACAAGA GACCACAGTT CAGGCATTCT CTCTTTTAGA ACTTGATGGC
    ATAAATCTCA GACTTGGAGA CAGGATATTC TGCAGCGCTT CTGTCTTTTT CTTGGAGAAT
    CCTCATGTAC AAAGTGTAGC CATCGAAAGC CAAGAATTTT TTGCAGGCTT TAAGCTACAG
    CCTGAATTGA GCACTATATC AGAGGATGGG AAAGAATACT ACCTGAGGAT AGAAAGCACA
    GTTCCTATTA TTTGTTCTGA ATTTAGTGAG CTTGATCAAG AATGCAAAAT CTCATTAAAA
    CTGAAAACTA TTGGTCAAGG TAGAGAGCAC CTAGGCTTAA ATTTGGCACT GTCTTCCTGT
    CATGTGGACC TTCTCCAGAC ATCTTCCTGT GCTAATGGAA CCTGTAGCCA CACTTTTGTG
    TACTACACTG CTGTCACAGA TTTTTCTCGA GATGGAGATA GAGTCTCAAA CATTGTAGTG
    CAACCAATAG TTAATGAGGA TTTCCTGTGG AACAACTACA TTCCAGACAG CATCCAGATC
    TGGTTTTCTT CTGGGGCATT TATCCGTGCT GATCTTGGTG AATGGGGCAT GAGTCTAACG
    ATCAGAGCCC CTAGTGTAGA TTACAGAAAC ACTCTGGGAC TTTGTGGAAC CTTTGATGAA
    AATCCGGAAA ATGATTTCCA TGACAAAAAT GGGATGCAAA TTGATCAAAA TTTTAACAAT
    TATGTTGCTT TTATTAATGA ATGGAGGATT TTACCAGGAA AAAGCATGTC TGACACACTG
    CCAGTTTCTA TGACATCACC TGGAAAGCCA TCCTATTGTA GCTGTTCATT GGACACTGCA
    GCGTATCCGT CTTCCGAAGA TCTGGACAGT GTTTCTCGAT CAGAAATTGC CTTGGGTTGC
    AAAGACCTCA ATCATGTCAG CTTATCTTCT TTGATACCAG AACTAGATGT CACCTCCGAA
    TATATTAATT CAGACACTCT TGTCAGAGAG ATAAACAAGC ATACATCTCC AGAAGAATAT
    AATCTAAATT TATTTCTGCA AGAAAAAAAA CACATAAACC TGACTAAACT CGGCTTAAAT
    GTACAAAAAC ATCCTGGAAA TGAGAAAGAA GATTCACTAC AATATTTGGC CAATAAGAAA
    TATACACAAG GCCGGGGAAG CCACAGCCAA GAAATGAGGT ACAATCGACA AAACAGATGG
    AAACGGCAGA ACTTTCATGA GTTTCCTCCT TTGTTTGCTT TCCCGAGTCT CAGCCAAACG
    GATCTGGAAG AACTTACTTA TTTTTTCCCA GAGGACCATG CTGAGGATGT ACAGCAAGAG
    TTTTTCCCCT CTTGGCCCAC TCCCTCTGGC CTCACCGAGT ATAGCACCTT GACCCTCTGT
    CAGGAGACTC TAGCCAACTC CAGCATAGGA AGGCTGTGTC TTGCTTTTCT TGGCAAGAGA
    TTAGACAGTG TTATAGAGAT GTGTGTGAAG GATGTTCTGT TAAAAGATGA TCTTAGTTGG
    GCAGAAGCAG GTGTGGCCCT TTTAGAAAAT GAATGTGAAA AGAGGATTGT GGAGGAGGGG
    AAATATAACA CAGAAGAGTA TGGCACATCA ATTGAAGACA TTCTCTCAGT ATTAAAATGC
    CCCAATTTAT GCAGCGGCAA TGGGCAGTGC ATGGAATGGG GGTGTGCGTG TTCCCCAAGC
    TTTAGTTCCT ATGACTGCAG TGATTCCTAT GACAAAGCTC CTGAAATTAC AGAGCTTGGG
    AATGCTGGAT TCTGTGATGT TCAAAAATAT AATTGTATGA TGGTGAGAGT TTTTGGCAAA
    GGCTTCAAAG AATTACCTTC AATTAAATGT GAAGTTACTA AGCTACAGTA TAATAGCAGT
    GAATGGATGC CTGGAGAACC CATCTATACA CAGACTGTTT TCCACAATAG CAGAGCTGTT
    GATTGTCAGC TGCCCACTGA TGTTCAGCAG TTTGATACCA TGGATCTGGT GGGTGGGAAA
    CCAACTGGGA AGTGGCAGTT GAAGGTATCT AATGATGGTT ATAAATTCAG TAATCCCAAA
    ATAACGGTCA TATATGATGG TGCTTGCCAA GTTTGTGGTC TCTATAAAAA TGACTCATGT
    ACTATAAAGG AAAATGTTTG CATTATTGAT GGACTCTGCT ATGTTGAAGG AGACAAAAAT
    CCAACCAGCC CTTGTTTGAT TTGTAGACCC AAAATTTCAA GATTTACTTG GTCATTTTTA
    GAAAACAACC AGCCCCCAGT GATTCAAGCA TTGCAAGACA AATTACAGAC ATTTTATGGT
    GAAAACTTTG AGTATCAGTT CGTGGCCTTC GATCCAGAAG GTTCTGACAT CCATTTTACG
    TTGGACTCTG GTCCTGAAGG GGCAAGTGTT TCCTCTGCAG GGCTTTTTAT GTGGAAAACA
    GATTTACTAA CTACCCAACA AATTACTGTA CGCCTTAATG ATGACTGCGA TGCTGAAACT
    AGAGTCACGA TTGAGGTGAC TGTGAAGTCT TGTGATTGCT TGAATGGTGG ATCATGTGTA
    TCTGATAGGA ACTTTTCTCC AGGGAGTGGA GTGTACCTGT GTGTCTGCTT GCCTGGCTTC
    CATGGCAGCC TTTGTGAAGT GGACATTAGT GGGTGCCAAT CCAACCCTTG TGGTCTTGGC
    AGCTATATTA GTGGTTTTCA CAGTTATTCC TGTGATTGTC CACCTGAGCT CAAAGTTGAA
    ACACAGTTTG TAAATCAATT TACTACACAA ACTGTGGTTC TCACAAGATC TGATAAAAGT
    GTAAATAAAG AAGAGGATGA TAAAAATGCC CAAGGGAGAA AGAGGCATGT TAAGCCAACA
    AGTGGAAATG CCTTCACCAT TTGTAAATAT CCATGTGGAA AAAGTAGGGA GTGTGTTGCC
    CCAAACATCT GCAAATGTAA ACCTGGTTAC ATTGGTTCTA ACTGCCAAAC TGCTCTTTGT
    GACCCTGATT GCAAAAACCA TGGAAAATGT ATTAAGCCTA ACATTTGTCA GTGTCTTCCA
    GGACATGGTG GAGCAACCTG TGATGAAGAA CATTGTAACC CACCTTGTCA ACATGGTGGC
    ACATGCTTGG CTGGGAATCT CTGCACTTGT CCTTATGGTT TTGTAGGACC AAGGTGTGAA
    ACAATGGTTT GTAACAGGCA CTGTGAAAAT GGAGGCCAGT GTCTTACACC AGATATTTGC
    CAGTGCAAAC CTGGCTGGTA TGGACCCACC TGTAGTACAG CTTTGTGCGA CCCTGTCTGC
    CTCAATGGTG GTTCGTGTAA TAAGCCAAAT ACTTGCCTCT GTCCAAATGG ATTCTTTGGG
    GAACACTGTC AGAATGCTTT CTGTCACCCT CCCTGTAAGA ATGGTGGCCA CTGCATGAGA
    AATAATGTGT GCGTCTGTCG TGAAGGATAC ACTGGTAGGA GATTCCAAAA AAGCATCTGT
    GATCCTACGT GCATGAATGG AGGAAAATGT GTGGGACCAA GCACTTGCTC TTGTCCTTCT
    GGTTGGAGTG GGAAACGATG CAACACACCT ATCTGCTTGC AGAAATGTAA AAACGGTGGT
    GAATGCATTG CGCCCAGCAT ATGCCATTGT CCTTCCTCCT GGGAAGGAGT GCGGTGTCAA
    ATACCAATTT GCAACCCAAA ATGTCTTTAT GGAGGCAGAT GCATATTTCC CAATGTGTGT
    TCCTGCCGCA CTGAATACTC TGGAGTCAAA TGTGAGAAGA AAATACAGAT AAGACGCCAT
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_016867318.1
    CDS322..4284
    Translation

    Target ORF information:

    RefSeq Version XM_017011829.1
    Organism Homo sapiens(human)
    Definition Homo sapiens von Willebrand factor D and EGF domains (VWDE), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017011829.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    ATGAAGAAGG CTATTTCTGA TGCACGATTA CACCCATGTG GTTCTGATGA AACTGAAACA 
    GGAGGTGATT GTGTTCGTCA GCTGGCTGCC TCATTGCCAC CTCCACCTGC AGGAAGGCCA
    GAGGTTCTGG TGGAGTTGAT TGAGTCCAGG CTTTTCTGTA GGTGTTCTTT TGATGTTCCC
    GCTACAAAAA ACTCAGTGGG ATTTCACATA GCTTGGTCTA GGCTTTCTTC TCAAGAAGTC
    AAAGAGGAGC TGACACAAGA GACCACAGTT CAGGCATTCT CTCTTTTAGA ACTTGATGGC
    ATAAATCTCA GACTTGGAGA CAGGATATTC TGCAGCGCTT CTGTCTTTTT CTTGGAGAAT
    CCTCATGTAC AAAGTGTAGC CATCGAAAGC CAAGAATTTT TTGCAGGCTT TAAGCTACAG
    CCTGAATTGA GCACTATATC AGAGGATGGG AAAGAATACT ACCTGAGGAT AGAAAGCACA
    GTTCCTATTA TTTGTTCTGA ATTTAGTGAG CTTGATCAAG AATGCAAAAT CTCATTAAAA
    CTGAAAACTA TTGGTCAAGG TAGAGAGCAC CTAGGCTTAA ATTTGGCACT GTCTTCCTGT
    CATGTGGACC TTCTCCAGAC ATCTTCCTGT GCTAATGGAA CCTGTAGCCA CACTTTTGTG
    TACTACACTG CTGTCACAGA TTTTTCTCGA GATGGAGATA GAGTCTCAAA CATTGTAGTG
    CAACCAATAG TTAATGAGGA TTTCCTGTGG AACAACTACA TTCCAGACAG CATCCAGATC
    TGGTTTTCTT CTGGGGCATT TATCCGTGCT GATCTTGGTG AATGGGGCAT GAGTCTAACG
    ATCAGAGCCC CTAGTGTAGA TTACAGAAAC ACTCTGGGAC TTTGTGGAAC CTTTGATGAA
    AATCCGGAAA ATGATTTCCA TGACAAAAAT GGGATGCAAA TTGATCAAAA TTTTAACAAT
    TATGTTGCTT TTATTAATGA ATGGAGGATT TTACCAGGAA AAAGCATGTC TGACACACTG
    CCAGTTTCTA TGACATCACC TGGAAAGCCA TCCTATTGTA GCTGTTCATT GGACACTGCA
    GCGTATCCGT CTTCCGAAGA TCTGGACAGT GTTTCTCGAT CAGAAATTGC CTTGGGTTGC
    AAAGACCTCA ATCATGTCAG CTTATCTTCT TTGATACCAG AACTAGATGT CACCTCCGAA
    TATATTAATT CAGACACTCT TGTCAGAGAG ATAAACAAGC ATACATCTCC AGAAGAATAT
    AATCTAAATT TATTTCTGCA AGAAAAAAAA CACATAAACC TGACTAAACT CGGCTTAAAT
    GTACAAAAAC ATCCTGGAAA TGAGAAAGAA GATTCACTAC AATATTTGGC CAATAAGAAA
    TATACACAAG GCCGGGGAAG CCACAGCCAA GAAATGAGGT ACAATCGACA AAACAGATGG
    AAACGGCAGA ACTTTCATGA GTTTCCTCCT TTGTTTGCTT TCCCGAGTCT CAGCCAAACG
    GATCTGGAAG AACTTACTTA TTTTTTCCCA GAGGACCATG CTGAGGATGT ACAGCAAGAG
    TTTTTCCCCT CTTGGCCCAC TCCCTCTGGC CTCACCGAGT ATAGCACCTT GACCCTCTGT
    CAGGAGACTC TAGCCAACTC CAGCATAGGA AGGCTGTGTC TTGCTTTTCT TGGCAAGAGA
    TTAGACAGTG TTATAGAGAT GTGTGTGAAG GATGTTCTGT TAAAAGATGA TCTTAGTTGG
    GCAGAAGCAG GTGTGGCCCT TTTAGAAAAT GAATGTGAAA AGAGGATTGT GGAGGAGGGG
    AAATATAACA CAGAAGAGTA TGGCACATCA ATTGAAGACA TTCTCTCAGT ATTAAAATGC
    CCCAATTTAT GCAGCGGCAA TGGGCAGTGC ATGGAATGGG GGTGTGCGTG TTCCCCAAGC
    TTTAGTTCCT ATGACTGCAG TGATTCCTAT GACAAAGCTC CTGAAATTAC AGAGCTTGGG
    AATGCTGGAT TCTGTGATGT TCAAAAATAT AATTGTATGA TGGTGAGAGT TTTTGGCAAA
    GGCTTCAAAG AATTACCTTC AATTAAATGT GAAGTTACTA AGCTACAGTA TAATAGCAGT
    GAATGGATGC CTGGAGAACC CATCTATACA CAGACTGTTT TCCACAATAG CAGAGCTGTT
    GATTGTCAGC TGCCCACTGA TGTTCAGCAG TTTGATACCA TGGATCTGGT GGGTGGGAAA
    CCAACTGGGA AGTGGCAGTT GAAGGTATCT AATGATGGTT ATAAATTCAG TAATCCCAAA
    ATAACGGTCA TATATGATGG TGCTTGCCAA GTTTGTGGTC TCTATAAAAA TGACTCATGT
    ACTATAAAGG AAAATGTTTG CATTATTGAT GGACTCTGCT ATGTTGAAGG AGACAAAAAT
    CCAACCAGCC CTTGTTTGAT TTGTAGACCC AAAATTTCAA GATTTACTTG GTCATTTTTA
    GAAAACAACC AGCCCCCAGT GATTCAAGCA TTGCAAGACA AATTACAGAC ATTTTATGGT
    GAAAACTTTG AGTATCAGTT CGTGGCCTTC GATCCAGAAG GTTCTGACAT CCATTTTACG
    TTGGACTCTG GTCCTGAAGG GGCAAGTGTT TCCTCTGCAG GGCTTTTTAT GTGGAAAACA
    GATTTACTAA CTACCCAACA AATTACTGTA CGCCTTAATG ATGACTGCGA TGCTGAAACT
    AGAGTCACGA TTGAGGTGAC TGTGAAGTCT TGTGATTGCT TGAATGGTGG ATCATGTGTA
    TCTGATAGGA ACTTTTCTCC AGGGAGTGGA GTGTACCTGT GTGTCTGCTT GCCTGGCTTC
    CATGGCAGCC TTTGTGAAGT GGACATTAGT GGGTGCCAAT CCAACCCTTG TGGTCTTGGC
    AGCTATATTA GTGGTTTTCA CAGTTATTCC TGTGATTGTC CACCTGAGCT CAAAGTTGAA
    ACACAGTTTG TAAATCAATT TACTACACAA ACTGTGGTTC TCACAAGATC TGATAAAAGT
    GTAAATAAAG AAGAGGATGA TAAAAATGCC CAAGGGAGAA AGAGGCATGT TAAGCCAACA
    AGTGGAAATG CCTTCACCAT TTGTAAATAT CCATGTGGAA AAAGTAGGGA GTGTGTTGCC
    CCAAACATCT GCAAATGTAA ACCTGGTTAC ATTGGTTCTA ACTGCCAAAC TGCTCTTTGT
    GACCCTGATT GCAAAAACCA TGGAAAATGT ATTAAGCCTA ACATTTGTCA GTGTCTTCCA
    GGACATGGTG GAGCAACCTG TGATGAAGAA CATTGTAACC CACCTTGTCA ACATGGTGGC
    ACATGCTTGG CTGGGAATCT CTGCACTTGT CCTTATGGTT TTGTAGGACC AAGGTGTGAA
    ACAATGGTTT GTAACAGGCA CTGTGAAAAT GGAGGCCAGT GTCTTACACC AGATATTTGC
    CAGTGCAAAC CTGGCTGGTA TGGACCCACC TGTAGTACAG CTTTGTGCGA CCCTGTCTGC
    CTCAATGGTG GTTCGTGTAA TAAGCCAAAT ACTTGCCTCT GTCCAAATGG ATTCTTTGGG
    GAACACTGTC AGAATGCTTT CTGTCACCCT CCCTGTAAGA ATGGTGGCCA CTGCATGAGA
    AATAATGTGT GCGTCTGTCG TGAAGGATAC ACTGGTAGGA GATTCCAAAA AAGCATCTGT
    GATCCTACGT GCATGAATGG AGGAAAATGT GTGGGACCAA GCACTTGCTC TTGTCCTTCT
    GGTTGGAGTG GGAAACGATG CAACACACCT ATCTGCTTGC AGAAATGTAA AAACGGTGGT
    GAATGCATTG CGCCCAGCAT ATGCCATTGT CCTTCCTCCT GGGAAGGAGT GCGGTGTCAA
    ATACCAATTT GCAACCCAAA ATGTCTTTAT GGAGGCAGAT GCATATTTCC CAATGTGTGT
    TCCTGCCGCA CTGAATACTC TGGAGTCAAA TGTGAGAAGA AAATACAGAT AAGACGCCAT
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu97083
    Clone ID Related Accession (Same CDS sequence) XM_017011830.1
    Accession Version XM_017011830.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3819bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product von Willebrand factor D and EGF domain-containing protein isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000007.14) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    ATGCCTGGCG GAGCCTGCGT GCTGGTGATC GCGCTGATGT TCCTGGCCTG GGGGGAAGCT 
    CAGGAGTGCT CTCCTGGGGG ACACCAGTTT CTTCGGAGTC CTTATAGAAG TGTCCGTTTT
    GACTCATGGC ACCTCCAGCA GTCAGCTGTT CAAGACCTAA TATGTGACCA TTCCCTCTCC
    CCTGGATGGT ATAGATTTCT CATCCTTGAC AGACCTGCCG AGATGCCAAC CAAATGTGTT
    GAGATGAACC ATTGTGGAAC TCAGGCCCCC ATCTGGCTGT CTCTGAGAGA TTCAGAAACA
    CTGCCATCTC CTGGGGAGAT CAAGCAATTG ACAGCTTGTG CAACATGGCA GTTTTTGTTC
    AGCACTACAA AAGACTGCTG TCTCTTTCAA ATCCCAGTGT CTGTAAGAAA CTGTGGGAAC
    TTTTCTGTAT ACTTACTACA ACCAACTCAG GGATGTATGG GCTACTGTGC GGAAGCTATT
    TCTGATGCAC GATTACACCC ATGTGGTTCT GATGAAACTG AAACAGGAGG TGATTGTGTT
    CGTCAGCTGG CTGCCTCATT GCCACCTCCA CCTGCAGGAA GGCCAGAGGT TCTGGTGGAG
    TTGATTGAGT CCAGGCTTTT CTGTAGGTGT TCTTTTGATG TTCCCGCTAC AAAAAACTCA
    GTGGGATTTC ACATAGCTTG GTCTAGGCTT TCTTCTCAAG AAGTCAAAGA GGAGCTGACA
    CAAGAGACCA CAGTTCAGGC ATTCTCTCTT TTAGAACTTG ATGGCATAAA TCTCAGACTT
    GGAGACAGGA TATTCTGCAG CGCTTCTGTC TTTTTCTTGG AGAATCCTCA TGTACAAAGT
    GTAGCCATCG AAAGCCAAGA ATTTTTTGCA GGCTTTAAGC TACAGCCTGA ATTGAGCACT
    ATATCAGAGG ATGGGAAAGA ATACTACCTG AGGATAGAAA GCACAGTTCC TATTATTTGT
    TCTGAATTTA GTGAGCTTGA TCAAGAATGC AAAATCTCAT TAAAACTGAA AACTATTGGT
    CAAGGTAGAG AGCACCTAGG CTTAAATTTG GCACTGTCTT CCTGTCATGT GGACCTTCTC
    CAGACATCTT CCTGTGCTAA TGGAACCTGT AGCCACACTT TTGTGTACTA CACTGCTGTC
    ACAGATTTTT CTCGAGATGG AGATAGAGTC TCAAACATTG TAGTGCAACC AATAGTTAAT
    GAGGATTTCC TGTGGAACAA CTACATTCCA GACAGCATCC AGATCAAAGT AAAGGATGTC
    CCAACTGCTT ACTGCTATAC ATTTACTGAC CCACATATAA TTACATTTGA TGGCAGGGTA
    TATGATAATT TCAAGACTGG AACATTTGTG CTTTATAAGA GTATGTCACG TGATTTTGAA
    GTCCATGTAC GTCAGTGGGA CTGCAGAAGC CTTCACTATC CAGTGTCATG TAATTGTGGG
    TTTGTTGCCC AGGAAGGAGG TGATATAGTT ACTTTTGATA TGTGCAATGG TCAGCTACGT
    GAATCACAAC CATATTTGTT CATAAAAAGC CAAGATGTAA CCAGGAATAT CAAGATAAGT
    GAATCTTACT TAGGAAGAAA AGTCACAATC TGGTTTTCTT CTGGGGCATT TATCCGTGCT
    GATCTTGGTG AATGGGGCAT GAGTCTAACG ATCAGAGCCC CTAGTGTAGA TTACAGAAAC
    ACTCTGGGAC TTTGTGGAAC CTTTGATGAA AATCCGGAAA ATGATTTCCA TGACAAAAAT
    GGGATGCAAA TTGATCAAAA TTTTAACAAT TATGTTGCTT TTATTAATGA ATGGAGGATT
    TTACCAGGAA AAAGCATGTC TGACACACTG CCAGTTTCTA TGACATCACC TGGAAAGCCA
    TCCTATTGTA GCTGTTCATT GGACACTGCA GCGTATCCGT CTTCCGAAGA TCTGGACAGT
    GTTTCTCGAT CAGAAATTGC CTTGGGTTGC AAAGACCTCA ATCATGTCAG CTTATCTTCT
    TTGATACCAG AACTAGATGT CACCTCCGAA TATATTAATT CAGACACTCT TGTCAGAGAG
    ATAAACAAGC ATACATCTCC AGAAGAATAT AATCTAAATT TATTTCTGCA AGAAAAAAAA
    CACATAAACC TGACTAAACT CGGCTTAAAT GTACAAAAAC ATCCTGGAAA TGAGAAAGAA
    GATTCACTAC AATATTTGGC CAATAAGAAA TATACACAAG GCCGGGGAAG CCACAGCCAA
    GAAATGAGGT ACAATCGACA AAACAGATGG AAACGGCAGA ACTTTCATGA GTTTCCTCCT
    TTGTTTGCTT TCCCGAGTCT CAGCCAAACG GATCTGGAAG AACTTACTTA TTTTTTCCCA
    GAGGACCATG CTGAGGATGT ACAGCAAGAG TTTTTCCCCT CTTGGCCCAC TCCCTCTGGC
    CTCACCGAGT ATAGCACCTT GACCCTCTGT CAGGAGACTC TAGCCAACTC CAGCATAGGA
    AGGCTGTGTC TTGCTTTTCT TGGCAAGAGA TTAGACAGTG TTATAGAGAT GTGTGTGAAG
    GATGTTCTGT TAAAAGATGA TCTTAGTTGG GCAGAAGCAG GTGTGGCCCT TTTAGAAAAT
    GAATGTGAAA AGAGGATTGT GGAGGAGGGG AAATATAACA CAGAAGAGTA TGGCACATCA
    ATTGAAGACA TTCTCTCAGT ATTAAAATGC CCCAATTTAT GCAGCGGCAA TGGGCAGTGC
    ATGGAATGGG GGTGTGCGTG TTCCCCAAGC TTTAGTTCCT ATGACTGCAG TGATTCCTAT
    GACAAAGCTC CTGAAATTAC AGAGCTTGGG AATGCTGGAT TCTGTGATGT TCAAAAATAT
    AATTGTATGA TGGTGAGAGT TTTTGGCAAA GGCTTCAAAG AATTACCTTC AATTAAATGT
    GAAGTTACTA AGCTACAGTA TAATAGCAGT GAATGGATGC CTGGAGAACC CATCTATACA
    CAGACTGTTT TCCACAATAG CAGAGCTGTT GATTGTCAGC TGCCCACTGA TGTTCAGCAG
    TTTGATACCA TGGATCTGGT GGGTGGGAAA CCAACTGGGA AGTGGCAGTT GAAGGTATCT
    AATGATGGTT ATAAATTCAG TAATCCCAAA ATAACGGTCA TATATGATGG TGCTTGCCAA
    GTTTGTGGTC TCTATAAAAA TGACTCATGT ACTATAAAGG AAAATGTTTG CATTATTGAT
    GGACTCTGCT ATGTTGAAGG AGACAAAAAT CCAACCAGCC CTTGTTTGAT TTGTAGACCC
    AAAATTTCAA GATTTACTTG GTCATTTTTA GAAAACAACC AGCCCCCAGT GATTCAAGCA
    TTGCAAGACA AATTACAGAC ATTTTATGGT GAAAACTTTG AGTATCAGTT CGTGGCCTTC
    GATCCAGAAG GTTCTGACAT CCATTTTACG TTGGACTCTG GTCCTGAAGG GGCAAGTGTT
    TCCTCTGCAG GGCTTTTTAT GTGGAAAACA GATTTACTAA CTACCCAACA AATTACTGTA
    CGCCTTAATG ATGACTGCGA TGCTGAAACT AGAGTCACGA TTGAGGTGAC TGTGAAGTCT
    TGTGATTGCT TGAATGGTGG ATCATGTGTA TCTGATAGGA ACTTTTCTCC AGGGAGTGGA
    GTGTACCTGT GTGTCTGCTT GCCTGGCTTC CATGGCAGCC TTTGTGAAGT GGACATTAGT
    GGGTGCCAAT CCAACCCTTG TGGTCTTGGC AGCTATATTA GTGGTTTTCA CAGTTATTCC
    TGTGATTGTC CACCTGAGCT CAAAGAAATC ACAAAAGGGC TAGTAGGAAT ACTACACAGG
    GAATCTTATC TACCTGGAGA AATAATGAAA AAGTTTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_016867319.1
    CDS188..4006
    Translation

    Target ORF information:

    RefSeq Version XM_017011830.1
    Organism Homo sapiens(human)
    Definition Homo sapiens von Willebrand factor D and EGF domains (VWDE), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017011830.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    ATGCCTGGCG GAGCCTGCGT GCTGGTGATC GCGCTGATGT TCCTGGCCTG GGGGGAAGCT 
    CAGGAGTGCT CTCCTGGGGG ACACCAGTTT CTTCGGAGTC CTTATAGAAG TGTCCGTTTT
    GACTCATGGC ACCTCCAGCA GTCAGCTGTT CAAGACCTAA TATGTGACCA TTCCCTCTCC
    CCTGGATGGT ATAGATTTCT CATCCTTGAC AGACCTGCCG AGATGCCAAC CAAATGTGTT
    GAGATGAACC ATTGTGGAAC TCAGGCCCCC ATCTGGCTGT CTCTGAGAGA TTCAGAAACA
    CTGCCATCTC CTGGGGAGAT CAAGCAATTG ACAGCTTGTG CAACATGGCA GTTTTTGTTC
    AGCACTACAA AAGACTGCTG TCTCTTTCAA ATCCCAGTGT CTGTAAGAAA CTGTGGGAAC
    TTTTCTGTAT ACTTACTACA ACCAACTCAG GGATGTATGG GCTACTGTGC GGAAGCTATT
    TCTGATGCAC GATTACACCC ATGTGGTTCT GATGAAACTG AAACAGGAGG TGATTGTGTT
    CGTCAGCTGG CTGCCTCATT GCCACCTCCA CCTGCAGGAA GGCCAGAGGT TCTGGTGGAG
    TTGATTGAGT CCAGGCTTTT CTGTAGGTGT TCTTTTGATG TTCCCGCTAC AAAAAACTCA
    GTGGGATTTC ACATAGCTTG GTCTAGGCTT TCTTCTCAAG AAGTCAAAGA GGAGCTGACA
    CAAGAGACCA CAGTTCAGGC ATTCTCTCTT TTAGAACTTG ATGGCATAAA TCTCAGACTT
    GGAGACAGGA TATTCTGCAG CGCTTCTGTC TTTTTCTTGG AGAATCCTCA TGTACAAAGT
    GTAGCCATCG AAAGCCAAGA ATTTTTTGCA GGCTTTAAGC TACAGCCTGA ATTGAGCACT
    ATATCAGAGG ATGGGAAAGA ATACTACCTG AGGATAGAAA GCACAGTTCC TATTATTTGT
    TCTGAATTTA GTGAGCTTGA TCAAGAATGC AAAATCTCAT TAAAACTGAA AACTATTGGT
    CAAGGTAGAG AGCACCTAGG CTTAAATTTG GCACTGTCTT CCTGTCATGT GGACCTTCTC
    CAGACATCTT CCTGTGCTAA TGGAACCTGT AGCCACACTT TTGTGTACTA CACTGCTGTC
    ACAGATTTTT CTCGAGATGG AGATAGAGTC TCAAACATTG TAGTGCAACC AATAGTTAAT
    GAGGATTTCC TGTGGAACAA CTACATTCCA GACAGCATCC AGATCAAAGT AAAGGATGTC
    CCAACTGCTT ACTGCTATAC ATTTACTGAC CCACATATAA TTACATTTGA TGGCAGGGTA
    TATGATAATT TCAAGACTGG AACATTTGTG CTTTATAAGA GTATGTCACG TGATTTTGAA
    GTCCATGTAC GTCAGTGGGA CTGCAGAAGC CTTCACTATC CAGTGTCATG TAATTGTGGG
    TTTGTTGCCC AGGAAGGAGG TGATATAGTT ACTTTTGATA TGTGCAATGG TCAGCTACGT
    GAATCACAAC CATATTTGTT CATAAAAAGC CAAGATGTAA CCAGGAATAT CAAGATAAGT
    GAATCTTACT TAGGAAGAAA AGTCACAATC TGGTTTTCTT CTGGGGCATT TATCCGTGCT
    GATCTTGGTG AATGGGGCAT GAGTCTAACG ATCAGAGCCC CTAGTGTAGA TTACAGAAAC
    ACTCTGGGAC TTTGTGGAAC CTTTGATGAA AATCCGGAAA ATGATTTCCA TGACAAAAAT
    GGGATGCAAA TTGATCAAAA TTTTAACAAT TATGTTGCTT TTATTAATGA ATGGAGGATT
    TTACCAGGAA AAAGCATGTC TGACACACTG CCAGTTTCTA TGACATCACC TGGAAAGCCA
    TCCTATTGTA GCTGTTCATT GGACACTGCA GCGTATCCGT CTTCCGAAGA TCTGGACAGT
    GTTTCTCGAT CAGAAATTGC CTTGGGTTGC AAAGACCTCA ATCATGTCAG CTTATCTTCT
    TTGATACCAG AACTAGATGT CACCTCCGAA TATATTAATT CAGACACTCT TGTCAGAGAG
    ATAAACAAGC ATACATCTCC AGAAGAATAT AATCTAAATT TATTTCTGCA AGAAAAAAAA
    CACATAAACC TGACTAAACT CGGCTTAAAT GTACAAAAAC ATCCTGGAAA TGAGAAAGAA
    GATTCACTAC AATATTTGGC CAATAAGAAA TATACACAAG GCCGGGGAAG CCACAGCCAA
    GAAATGAGGT ACAATCGACA AAACAGATGG AAACGGCAGA ACTTTCATGA GTTTCCTCCT
    TTGTTTGCTT TCCCGAGTCT CAGCCAAACG GATCTGGAAG AACTTACTTA TTTTTTCCCA
    GAGGACCATG CTGAGGATGT ACAGCAAGAG TTTTTCCCCT CTTGGCCCAC TCCCTCTGGC
    CTCACCGAGT ATAGCACCTT GACCCTCTGT CAGGAGACTC TAGCCAACTC CAGCATAGGA
    AGGCTGTGTC TTGCTTTTCT TGGCAAGAGA TTAGACAGTG TTATAGAGAT GTGTGTGAAG
    GATGTTCTGT TAAAAGATGA TCTTAGTTGG GCAGAAGCAG GTGTGGCCCT TTTAGAAAAT
    GAATGTGAAA AGAGGATTGT GGAGGAGGGG AAATATAACA CAGAAGAGTA TGGCACATCA
    ATTGAAGACA TTCTCTCAGT ATTAAAATGC CCCAATTTAT GCAGCGGCAA TGGGCAGTGC
    ATGGAATGGG GGTGTGCGTG TTCCCCAAGC TTTAGTTCCT ATGACTGCAG TGATTCCTAT
    GACAAAGCTC CTGAAATTAC AGAGCTTGGG AATGCTGGAT TCTGTGATGT TCAAAAATAT
    AATTGTATGA TGGTGAGAGT TTTTGGCAAA GGCTTCAAAG AATTACCTTC AATTAAATGT
    GAAGTTACTA AGCTACAGTA TAATAGCAGT GAATGGATGC CTGGAGAACC CATCTATACA
    CAGACTGTTT TCCACAATAG CAGAGCTGTT GATTGTCAGC TGCCCACTGA TGTTCAGCAG
    TTTGATACCA TGGATCTGGT GGGTGGGAAA CCAACTGGGA AGTGGCAGTT GAAGGTATCT
    AATGATGGTT ATAAATTCAG TAATCCCAAA ATAACGGTCA TATATGATGG TGCTTGCCAA
    GTTTGTGGTC TCTATAAAAA TGACTCATGT ACTATAAAGG AAAATGTTTG CATTATTGAT
    GGACTCTGCT ATGTTGAAGG AGACAAAAAT CCAACCAGCC CTTGTTTGAT TTGTAGACCC
    AAAATTTCAA GATTTACTTG GTCATTTTTA GAAAACAACC AGCCCCCAGT GATTCAAGCA
    TTGCAAGACA AATTACAGAC ATTTTATGGT GAAAACTTTG AGTATCAGTT CGTGGCCTTC
    GATCCAGAAG GTTCTGACAT CCATTTTACG TTGGACTCTG GTCCTGAAGG GGCAAGTGTT
    TCCTCTGCAG GGCTTTTTAT GTGGAAAACA GATTTACTAA CTACCCAACA AATTACTGTA
    CGCCTTAATG ATGACTGCGA TGCTGAAACT AGAGTCACGA TTGAGGTGAC TGTGAAGTCT
    TGTGATTGCT TGAATGGTGG ATCATGTGTA TCTGATAGGA ACTTTTCTCC AGGGAGTGGA
    GTGTACCTGT GTGTCTGCTT GCCTGGCTTC CATGGCAGCC TTTGTGAAGT GGACATTAGT
    GGGTGCCAAT CCAACCCTTG TGGTCTTGGC AGCTATATTA GTGGTTTTCA CAGTTATTCC
    TGTGATTGTC CACCTGAGCT CAAAGAAATC ACAAAAGGGC TAGTAGGAAT ACTACACAGG
    GAATCTTATC TACCTGGAGA AATAATGAAA AAGTTTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu41093
    Clone ID Related Accession (Same CDS sequence) XM_005249641.1
    Accession Version XM_005249641.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3135bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product von Willebrand factor D and EGF domain-containing protein isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000007.14) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    ATGAGTCTAA CGATCAGAGC CCCTAGTGTA GATTACAGAA ACACTCTGGG ACTTTGTGGA 
    ACCTTTGATG AAAATCCGGA AAATGATTTC CATGACAAAA ATGGGATGCA AATTGATCAA
    AATTTTAACA ATTATGTTGC TTTTATTAAT GAATGGAGGA TTTTACCAGG AAAAAGCATG
    TCTGACACAC TGCCAGTTTC TATGACATCA CCTGGAAAGC CATCCTATTG TAGCTGTTCA
    TTGGACACTG CAGCGTATCC GTCTTCCGAA GATCTGGACA GTGTTTCTCG ATCAGAAATT
    GCCTTGGGTT GCAAAGACCT CAATCATGTC AGCTTATCTT CTTTGATACC AGAACTAGAT
    GTCACCTCCG AATATATTAA TTCAGACACT CTTGTCAGAG AGATAAACAA GCATACATCT
    CCAGAAGAAT ATAATCTAAA TTTATTTCTG CAAGAAAAAA AACACATAAA CCTGACTAAA
    CTCGGCTTAA ATGTACAAAA ACATCCTGGA AATGAGAAAG AAGATTCACT ACAATATTTG
    GCCAATAAGA AATATACACA AGGCCGGGGA AGCCACAGCC AAGAAATGAG GTACAATCGA
    CAAAACAGAT GGAAACGGCA GAACTTTCAT GAGTTTCCTC CTTTGTTTGC TTTCCCGAGT
    CTCAGCCAAA CGGATCTGGA AGAACTTACT TATTTTTTCC CAGAGGACCA TGCTGAGGAT
    GTACAGCAAG AGTTTTTCCC CTCTTGGCCC ACTCCCTCTG GCCTCACCGA GTATAGCACC
    TTGACCCTCT GTCAGGAGAC TCTAGCCAAC TCCAGCATAG GAAGGCTGTG TCTTGCTTTT
    CTTGGCAAGA GATTAGACAG TGTTATAGAG ATGTGTGTGA AGGATGTTCT GTTAAAAGAT
    GATCTTAGTT GGGCAGAAGC AGGTGTGGCC CTTTTAGAAA ATGAATGTGA AAAGAGGATT
    GTGGAGGAGG GGAAATATAA CACAGAAGAG TATGGCACAT CAATTGAAGA CATTCTCTCA
    GTATTAAAAT GCCCCAATTT ATGCAGCGGC AATGGGCAGT GCATGGAATG GGGGTGTGCG
    TGTTCCCCAA GCTTTAGTTC CTATGACTGC AGTGATTCCT ATGACAAAGC TCCTGAAATT
    ACAGAGCTTG GGAATGCTGG ATTCTGTGAT GTTCAAAAAT ATAATTGTAT GATGGTGAGA
    GTTTTTGGCA AAGGCTTCAA AGAATTACCT TCAATTAAAT GTGAAGTTAC TAAGCTACAG
    TATAATAGCA GTGAATGGAT GCCTGGAGAA CCCATCTATA CACAGACTGT TTTCCACAAT
    AGCAGAGCTG TTGATTGTCA GCTGCCCACT GATGTTCAGC AGTTTGATAC CATGGATCTG
    GTGGGTGGGA AACCAACTGG GAAGTGGCAG TTGAAGGTAT CTAATGATGG TTATAAATTC
    AGTAATCCCA AAATAACGGT CATATATGAT GGTGCTTGCC AAGTTTGTGG TCTCTATAAA
    AATGACTCAT GTACTATAAA GGAAAATGTT TGCATTATTG ATGGACTCTG CTATGTTGAA
    GGAGACAAAA ATCCAACCAG CCCTTGTTTG ATTTGTAGAC CCAAAATTTC AAGATTTACT
    TGGTCATTTT TAGAAAACAA CCAGCCCCCA GTGATTCAAG CATTGCAAGA CAAATTACAG
    ACATTTTATG GTGAAAACTT TGAGTATCAG TTCGTGGCCT TCGATCCAGA AGGTTCTGAC
    ATCCATTTTA CGTTGGACTC TGGTCCTGAA GGGGCAAGTG TTTCCTCTGC AGGGCTTTTT
    ATGTGGAAAA CAGATTTACT AACTACCCAA CAAATTACTG TACGCCTTAA TGATGACTGC
    GATGCTGAAA CTAGAGTCAC GATTGAGGTG ACTGTGAAGT CTTGTGATTG CTTGAATGGT
    GGATCATGTG TATCTGATAG GAACTTTTCT CCAGGGAGTG GAGTGTACCT GTGTGTCTGC
    TTGCCTGGCT TCCATGGCAG CCTTTGTGAA GTGGACATTA GTGGGTGCCA ATCCAACCCT
    TGTGGTCTTG GCAGCTATAT TAGTGGTTTT CACAGTTATT CCTGTGATTG TCCACCTGAG
    CTCAAAGTTG AAACACAGTT TGTAAATCAA TTTACTACAC AAACTGTGGT TCTCACAAGA
    TCTGATAAAA GTGTAAATAA AGAAGAGGAT GATAAAAATG CCCAAGGGAG AAAGAGGCAT
    GTTAAGCCAA CAAGTGGAAA TGCCTTCACC ATTTGTAAAT ATCCATGTGG AAAAAGTAGG
    GAGTGTGTTG CCCCAAACAT CTGCAAATGT AAACCTGGTT ACATTGGTTC TAACTGCCAA
    ACTGCTCTTT GTGACCCTGA TTGCAAAAAC CATGGAAAAT GTATTAAGCC TAACATTTGT
    CAGTGTCTTC CAGGACATGG TGGAGCAACC TGTGATGAAG AACATTGTAA CCCACCTTGT
    CAACATGGTG GCACATGCTT GGCTGGGAAT CTCTGCACTT GTCCTTATGG TTTTGTAGGA
    CCAAGGTGTG AAACAATGGT TTGTAACAGG CACTGTGAAA ATGGAGGCCA GTGTCTTACA
    CCAGATATTT GCCAGTGCAA ACCTGGCTGG TATGGACCCA CCTGTAGTAC AGCTTTGTGC
    GACCCTGTCT GCCTCAATGG TGGTTCGTGT AATAAGCCAA ATACTTGCCT CTGTCCAAAT
    GGATTCTTTG GGGAACACTG TCAGAATGCT TTCTGTCACC CTCCCTGTAA GAATGGTGGC
    CACTGCATGA GAAATAATGT GTGCGTCTGT CGTGAAGGAT ACACTGGTAG GAGATTCCAA
    AAAAGCATCT GTGATCCTAC GTGCATGAAT GGAGGAAAAT GTGTGGGACC AAGCACTTGC
    TCTTGTCCTT CTGGTTGGAG TGGGAAACGA TGCAACACAC CTATCTGCTT GCAGAAATGT
    AAAAACGGTG GTGAATGCAT TGCGCCCAGC ATATGCCATT GTCCTTCCTC CTGGGAAGGA
    GTGCGGTGTC AAATACCAAT TTGCAACCCA AAATGTCTTT ATGGAGGCAG ATGCATATTT
    CCCAATGTGT GTTCCTGCCG CACTGAATAC TCTGGAGTCA AATGTGAGAA GAAAATACAG
    ATAAGACGCC ATTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005249698.1
    CDS1263..4397
    Translation

    Target ORF information:

    RefSeq Version XM_005249641.1
    Organism Homo sapiens(human)
    Definition Homo sapiens von Willebrand factor D and EGF domains (VWDE), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005249641.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    ATGAGTCTAA CGATCAGAGC CCCTAGTGTA GATTACAGAA ACACTCTGGG ACTTTGTGGA 
    ACCTTTGATG AAAATCCGGA AAATGATTTC CATGACAAAA ATGGGATGCA AATTGATCAA
    AATTTTAACA ATTATGTTGC TTTTATTAAT GAATGGAGGA TTTTACCAGG AAAAAGCATG
    TCTGACACAC TGCCAGTTTC TATGACATCA CCTGGAAAGC CATCCTATTG TAGCTGTTCA
    TTGGACACTG CAGCGTATCC GTCTTCCGAA GATCTGGACA GTGTTTCTCG ATCAGAAATT
    GCCTTGGGTT GCAAAGACCT CAATCATGTC AGCTTATCTT CTTTGATACC AGAACTAGAT
    GTCACCTCCG AATATATTAA TTCAGACACT CTTGTCAGAG AGATAAACAA GCATACATCT
    CCAGAAGAAT ATAATCTAAA TTTATTTCTG CAAGAAAAAA AACACATAAA CCTGACTAAA
    CTCGGCTTAA ATGTACAAAA ACATCCTGGA AATGAGAAAG AAGATTCACT ACAATATTTG
    GCCAATAAGA AATATACACA AGGCCGGGGA AGCCACAGCC AAGAAATGAG GTACAATCGA
    CAAAACAGAT GGAAACGGCA GAACTTTCAT GAGTTTCCTC CTTTGTTTGC TTTCCCGAGT
    CTCAGCCAAA CGGATCTGGA AGAACTTACT TATTTTTTCC CAGAGGACCA TGCTGAGGAT
    GTACAGCAAG AGTTTTTCCC CTCTTGGCCC ACTCCCTCTG GCCTCACCGA GTATAGCACC
    TTGACCCTCT GTCAGGAGAC TCTAGCCAAC TCCAGCATAG GAAGGCTGTG TCTTGCTTTT
    CTTGGCAAGA GATTAGACAG TGTTATAGAG ATGTGTGTGA AGGATGTTCT GTTAAAAGAT
    GATCTTAGTT GGGCAGAAGC AGGTGTGGCC CTTTTAGAAA ATGAATGTGA AAAGAGGATT
    GTGGAGGAGG GGAAATATAA CACAGAAGAG TATGGCACAT CAATTGAAGA CATTCTCTCA
    GTATTAAAAT GCCCCAATTT ATGCAGCGGC AATGGGCAGT GCATGGAATG GGGGTGTGCG
    TGTTCCCCAA GCTTTAGTTC CTATGACTGC AGTGATTCCT ATGACAAAGC TCCTGAAATT
    ACAGAGCTTG GGAATGCTGG ATTCTGTGAT GTTCAAAAAT ATAATTGTAT GATGGTGAGA
    GTTTTTGGCA AAGGCTTCAA AGAATTACCT TCAATTAAAT GTGAAGTTAC TAAGCTACAG
    TATAATAGCA GTGAATGGAT GCCTGGAGAA CCCATCTATA CACAGACTGT TTTCCACAAT
    AGCAGAGCTG TTGATTGTCA GCTGCCCACT GATGTTCAGC AGTTTGATAC CATGGATCTG
    GTGGGTGGGA AACCAACTGG GAAGTGGCAG TTGAAGGTAT CTAATGATGG TTATAAATTC
    AGTAATCCCA AAATAACGGT CATATATGAT GGTGCTTGCC AAGTTTGTGG TCTCTATAAA
    AATGACTCAT GTACTATAAA GGAAAATGTT TGCATTATTG ATGGACTCTG CTATGTTGAA
    GGAGACAAAA ATCCAACCAG CCCTTGTTTG ATTTGTAGAC CCAAAATTTC AAGATTTACT
    TGGTCATTTT TAGAAAACAA CCAGCCCCCA GTGATTCAAG CATTGCAAGA CAAATTACAG
    ACATTTTATG GTGAAAACTT TGAGTATCAG TTCGTGGCCT TCGATCCAGA AGGTTCTGAC
    ATCCATTTTA CGTTGGACTC TGGTCCTGAA GGGGCAAGTG TTTCCTCTGC AGGGCTTTTT
    ATGTGGAAAA CAGATTTACT AACTACCCAA CAAATTACTG TACGCCTTAA TGATGACTGC
    GATGCTGAAA CTAGAGTCAC GATTGAGGTG ACTGTGAAGT CTTGTGATTG CTTGAATGGT
    GGATCATGTG TATCTGATAG GAACTTTTCT CCAGGGAGTG GAGTGTACCT GTGTGTCTGC
    TTGCCTGGCT TCCATGGCAG CCTTTGTGAA GTGGACATTA GTGGGTGCCA ATCCAACCCT
    TGTGGTCTTG GCAGCTATAT TAGTGGTTTT CACAGTTATT CCTGTGATTG TCCACCTGAG
    CTCAAAGTTG AAACACAGTT TGTAAATCAA TTTACTACAC AAACTGTGGT TCTCACAAGA
    TCTGATAAAA GTGTAAATAA AGAAGAGGAT GATAAAAATG CCCAAGGGAG AAAGAGGCAT
    GTTAAGCCAA CAAGTGGAAA TGCCTTCACC ATTTGTAAAT ATCCATGTGG AAAAAGTAGG
    GAGTGTGTTG CCCCAAACAT CTGCAAATGT AAACCTGGTT ACATTGGTTC TAACTGCCAA
    ACTGCTCTTT GTGACCCTGA TTGCAAAAAC CATGGAAAAT GTATTAAGCC TAACATTTGT
    CAGTGTCTTC CAGGACATGG TGGAGCAACC TGTGATGAAG AACATTGTAA CCCACCTTGT
    CAACATGGTG GCACATGCTT GGCTGGGAAT CTCTGCACTT GTCCTTATGG TTTTGTAGGA
    CCAAGGTGTG AAACAATGGT TTGTAACAGG CACTGTGAAA ATGGAGGCCA GTGTCTTACA
    CCAGATATTT GCCAGTGCAA ACCTGGCTGG TATGGACCCA CCTGTAGTAC AGCTTTGTGC
    GACCCTGTCT GCCTCAATGG TGGTTCGTGT AATAAGCCAA ATACTTGCCT CTGTCCAAAT
    GGATTCTTTG GGGAACACTG TCAGAATGCT TTCTGTCACC CTCCCTGTAA GAATGGTGGC
    CACTGCATGA GAAATAATGT GTGCGTCTGT CGTGAAGGAT ACACTGGTAG GAGATTCCAA
    AAAAGCATCT GTGATCCTAC GTGCATGAAT GGAGGAAAAT GTGTGGGACC AAGCACTTGC
    TCTTGTCCTT CTGGTTGGAG TGGGAAACGA TGCAACACAC CTATCTGCTT GCAGAAATGT
    AAAAACGGTG GTGAATGCAT TGCGCCCAGC ATATGCCATT GTCCTTCCTC CTGGGAAGGA
    GTGCGGTGTC AAATACCAAT TTGCAACCCA AAATGTCTTT ATGGAGGCAG ATGCATATTT
    CCCAATGTGT GTTCCTGCCG CACTGAATAC TCTGGAGTCA AATGTGAGAA GAAAATACAG
    ATAAGACGCC ATTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu78060
    Clone ID Related Accession (Same CDS sequence) XM_011515174.1
    Accession Version XM_011515174.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5133bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product von Willebrand factor D and EGF domain-containing protein isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000007.14) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    ATGCCTGGCG GAGCCTGCGT GCTGGTGATC GCGCTGATGT TCCTGGCCTG GGGGGAAGCT 
    CAGGAGTGCT CTCCTGGGGG ACACCAGTTT CTTCGGAGTC CTTATAGAAG TGTCCGTTTT
    GACTCATGGC ACCTCCAGCA GTCAGCTGTT CAAGACCTAA TATGTGACCA TTCCCTCTCC
    CCTGGATGGT ATAGATTTCT CATCCTTGAC AGACCTGCCG AGATGCCAAC CAAATGTGTT
    GAGATGAACC ATTGTGGAAC TCAGGCCCCC ATCTGGCTGT CTCTGAGAGA TTCAGAAACA
    CTGCCATCTC CTGGGGAGAT CAAGCAATTG ACAGCTTGTG CAACATGGCA GTTTTTGTTC
    AGCACTACAA AAGACTGCTG TCTCTTTCAA ATCCCAGTGT CTGTAAGAAA CTGTGGGAAC
    TTTTCTGTAT ACTTACTACA ACCAACTCAG GGATGTATGG GCTACTGTGC GGAAGCTATT
    TCTGATGCAC GATTACACCC ATGTGGTTCT GATGAAACTG AAACAGGAGG TGATTGTGTT
    CGTCAGCTGG CTGCCTCATT GCCACCTCCA CCTGCAGGAA GGCCAGAGGT TCTGGTGGAG
    TTGATTGAGT CCAGGCTTTT CTGTAGGTGT TCTTTTGATG TTCCCGCTAC AAAAAACTCA
    GTGGGATTTC ACATAGCTTG GTCTAGGCTT TCTTCTCAAG AAGTCAAAGA GGAGCTGACA
    CAAGAGACCA CAGTTCAGGC ATTCTCTCTT TTAGAACTTG ATGGCATAAA TCTCAGACTT
    GGAGACAGGA TATTCTGCAG CGCTTCTGTC TTTTTCTTGG AGAATCCTCA TGTACAAAGT
    GTAGCCATCG AAAGCCAAGA ATTTTTTGCA GGCTTTAAGC TACAGCCTGA ATTGAGCACT
    ATATCAGAGG ATGGGAAAGA ATACTACCTG AGGATAGAAA GCACAGTTCC TATTATTTGT
    TCTGAATTTA GTGAGCTTGA TCAAGAATGC AAAATCTCAT TAAAACTGAA AACTATTGGT
    CAAGGTAGAG AGCACCTAGG CTTAAATTTG GCACTGTCTT CCTGTCATGT GGACCTTCTC
    CAGACATCTT CCTGTGCTAA TGGAACCTGT AGCCACACTT TTGTGTACTA CACTGCTGTC
    ACAGATTTTT CTCGAGATGG AGATAGAGTC TCAAACATTG TAGTGCAACC AATAGTTAAT
    GAGGATTTCC TGTGGAACAA CTACATTCCA GACAGCATCC AGATCAAAGT AAAGGATGTC
    CCAACTGCTT ACTGCTATAC ATTTACTGAC CCACATATAA TTACATTTGA TGGCAGGGTA
    TATGATAATT TCAAGACTGG AACATTTGTG CTTTATAAGA GTATGTCACG TGATTTTGAA
    GTCCATGTAC GTCAGTGGGA CTGCAGAAGC CTTCACTATC CAGTGTCATG TAATTGTGGG
    TTTGTTGCCC AGGAAGGAGG TGATATAGTT ACTTTTGATA TGTGCAATGG TCAGCTACGT
    GAATCACAAC CATATTTGTT CATAAAAAGC CAAGATGTAA CCAGGAATAT CAAGATAAGT
    GAATCTTACT TAGGAAGAAA AGTCACAATC TGGTTTTCTT CTGGGGCATT TATCCGTGCT
    GATCTTGGTG AATGGGGCAT GAGTCTAACG ATCAGAGCCC CTAGTGTAGA TTACAGAAAC
    ACTCTGGGAC TTTGTGGAAC CTTTGATGAA AATCCGGAAA ATGATTTCCA TGACAAAAAT
    GGGATGCAAA TTGATCAAAA TTTTAACAAT TATGTTGCTT TTATTAATGA ATGGAGGATT
    TTACCAGGAA AAAGCATGTC TGACACACTG CCAGTTTCTA TGACATCACC TGGAAAGCCA
    TCCTATTGTA GCTGTTCATT GGACACTGCA GCGTATCCGT CTTCCGAAGA TCTGGACAGT
    GTTTCTCGAT CAGAAATTGC CTTGGGTTGC AAAGACCTCA ATCATGTCAG CTTATCTTCT
    TTGATACCAG AACTAGATGT CACCTCCGAA TATATTAATT CAGACACTCT TGTCAGAGAG
    ATAAACAAGC ATACATCTCC AGAAGAATAT AATCTAAATT TATTTCTGCA AGAAAAAAAA
    CACATAAACC TGACTAAACT CGGCTTAAAT GTACAAAAAC ATCCTGGAAA TGAGAAAGAA
    GATTCACTAC AATATTTGGC CAATAAGAAA TATACACAAG GCCGGGGAAG CCACAGCCAA
    GAAATGAGGT ACAATCGACA AAACAGATGG AAACGGCAGA ACTTTCATGA GTTTCCTCCT
    TTGTTTGCTT TCCCGAGTCT CAGCCAAACG GATCTGGAAG AACTTACTTA TTTTTTCCCA
    GAGGACCATG CTGAGGATGT ACAGCAAGAG TTTTTCCCCT CTTGGCCCAC TCCCTCTGGC
    CTCACCGAGT ATAGCACCTT GACCCTCTGT CAGGAGACTC TAGCCAACTC CAGCATAGGA
    AGGCTGTGTC TTGCTTTTCT TGGCAAGAGA TTAGACAGTG TTATAGAGAT GTGTGTGAAG
    GATGTTCTGT TAAAAGATGA TCTTAGTTGG GCAGAAGCAG GTGTGGCCCT TTTAGAAAAT
    GAATGTGAAA AGAGGATTGT GGAGGAGGGG AAATATAACA CAGAAGAGTA TGGCACATCA
    ATTGAAGACA TTCTCTCAGT ATTAAAATGC CCCAATTTAT GCAGCGGCAA TGGGCAGTGC
    ATGGAATGGG GGTGTGCGTG TTCCCCAAGC TTTAGTTCCT ATGACTGCAG TGATTCCTAT
    GACAAAGCTC CTGAAATTAC AGAGCTTGGG AATGCTGGAT TCTGTGATGT TCAAAAATAT
    AATTGTATGA TGGTGAGAGT TTTTGGCAAA GGCTTCAAAG AATTACCTTC AATTAAATGT
    GAAGTTACTA AGCTACAGTA TAATAGCAGT GAATGGATGC CTGGAGAACC CATCTATACA
    CAGACTGTTT TCCACAATAG CAGAGCTGTT GATTGTCAGC TGCCCACTGA TGTTCAGCAG
    TTTGATACCA TGGATCTGGT GGGTGGGAAA CCAACTGGGA AGTGGCAGTT GAAGGTATCT
    AATGATGGTT ATAAATTCAG TAATCCCAAA ATAACGGTCA TATATGATGG TGCTTGCCAA
    GTTTGTGGTC TCTATAAAAA TGACTCATGT ACTATAAAGG AAAATGTTTG CATTATTGAT
    GGACTCTGCT ATGTTGAAGG AGACAAAAAT CCAACCAGCC CTTGTTTGAT TTGTAGACCC
    AAAATTTCAA GATTTACTTG GTCATTTTTA GAAAACAACC AGCCCCCAGT GATTCAAGCA
    TTGCAAGACA AATTACAGAC ATTTTATGGT GAAAACTTTG AGTATCAGTT CGTGGCCTTC
    GATCCAGAAG GTTCTGACAT CCATTTTACG TTGGACTCTG GTCCTGAAGG GGCAAGTGTT
    TCCTCTGCAG GGCTTTTTAT GTGGAAAACA GATTTACTAA CTACCCAACA AATTACTGTA
    CGCCTTAATG ATGACTGCGA TGCTGAAACT AGAGTCACGA TTGAGGTGAC TGTGAAGTCT
    TGTGATTGCT TGAATGGTGG ATCATGTGTA TCTGATAGGA ACTTTTCTCC AGGGAGTGGA
    GTGTACCTGT GTGTCTGCTT GCCTGGCTTC CATGGCAGCC TTTGTGAAGT GGACATTAGT
    GGGTGCCAAT CCAACCCTTG TGGTCTTGGC AGCTATATTA GTGGTTTTCA CAGTTATTCC
    TGTGATTGTC CACCTGAGCT CAAAGGCAAA AATTGCCAGG AAGATGTTGA TGAGTGTGAC
    TCCAAGCCTT GCTTTGAGGG AGTAGCATGC CTGAATACCT TTGGTTCCTA TCATTGTGGT
    TCATGTCCAG AAGGATTTTA TGGGGATGGA AAAATATGCC ATGTTGAAAC ACAGTTTGTA
    AATCAATTTA CTACACAAAC TGTGGTTCTC ACAAGATCTG ATAAAAGTGT AAATAAAGAA
    GAGGATGATA AAAATGCCCA AGGGAGAAAG AGGCATGTTA AGCCAACAAG TGGAAATGCC
    TTCACTATAA ATTTTCAGCC ACCATCAACA GAATATTCAC TAATTAGTAC AAGATTTACT
    CCTACTCCCG CTACTATCAA AAAGTTACTG AGCACAACTA ACAACATCTC CCGCTCATCA
    CTGAAATTAA TTACAGCCCA AATGGATAAT TCAGTAAAGC CTATTTCCCA TCAGATATCA
    GATATTGAAC ATGTGGATCG TAGTCTCAGT ACAAATCTCA GTCTGGCCAT TTGTAAATAT
    CCATGTGGAA AAAGTAGGGA GTGTGTTGCC CCAAACATCT GCAAATGTAA ACCTGGTTAC
    ATTGGTTCTA ACTGCCAAAC TGCTCTTTGT GACCCTGATT GCAAAAACCA TGGAAAATGT
    ATTAAGCCTA ACATTTGTCA GTGTCTTCCA GGACATGGTG GAGCAACCTG TGATGAAGAA
    CATTGTAACC CACCTTGTCA ACATGGTGGC ACATGCTTGG CTGGGAATCT CTGCACTTGT
    CCTTATGGTT TTGTAGGACC AAGGTGTGAA ACAATGGTTT GTAACAGGCA CTGTGAAAAT
    GGAGGCCAGT GTCTTACACC AGATATTTGC CAGTGCAAAC CTGGCTGGTA TGGACCCACC
    TGTAGTACAG CTTTGTGCGA CCCTGTCTGC CTCAATGGTG GTTCGTGTAA TAAGCCAAAT
    ACTTGCCTCT GTCCAAATGG ATTCTTTGGG GAACACTGTC AGAATGCTTT CTGTCACCCT
    CCCTGTAAGA ATGGTGGCCA CTGCATGAGA AATAATGTGT GCGTCTGTCG TGAAGGATAC
    ACTGGTAGGA GATTCCAAAA AAGCATCTGT GATCCTACGT GCATGAATGG AGGAAAATGT
    GTGGGACCAA GCACTTGCTC TTGTCCTTCT GGTTGGAGTG GGAAACGATG CAACACACCT
    ATCTGCTTGC AGAAATGTAA AAACGGTGGT GAATGCATTG CGCCCAGCAT ATGCCATTGT
    CCTTCCTCCT GGGAAGGAGT GCGGTGTCAA ATACCAATTT GCAACCCAAA ATGTCTTTAT
    GGAGGCAGAT GCATATTTCC CAATGTGTGT TCCTGCCGCA CTGAATACTC TGGAGTCAAA
    TGTGAGAAGA AAATACAGAT AAGACGCCAT TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_011513476.1
    CDS188..5320
    Translation

    Target ORF information:

    RefSeq Version XM_011515174.1
    Organism Homo sapiens(human)
    Definition Homo sapiens von Willebrand factor D and EGF domains (VWDE), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_011515174.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    ATGCCTGGCG GAGCCTGCGT GCTGGTGATC GCGCTGATGT TCCTGGCCTG GGGGGAAGCT 
    CAGGAGTGCT CTCCTGGGGG ACACCAGTTT CTTCGGAGTC CTTATAGAAG TGTCCGTTTT
    GACTCATGGC ACCTCCAGCA GTCAGCTGTT CAAGACCTAA TATGTGACCA TTCCCTCTCC
    CCTGGATGGT ATAGATTTCT CATCCTTGAC AGACCTGCCG AGATGCCAAC CAAATGTGTT
    GAGATGAACC ATTGTGGAAC TCAGGCCCCC ATCTGGCTGT CTCTGAGAGA TTCAGAAACA
    CTGCCATCTC CTGGGGAGAT CAAGCAATTG ACAGCTTGTG CAACATGGCA GTTTTTGTTC
    AGCACTACAA AAGACTGCTG TCTCTTTCAA ATCCCAGTGT CTGTAAGAAA CTGTGGGAAC
    TTTTCTGTAT ACTTACTACA ACCAACTCAG GGATGTATGG GCTACTGTGC GGAAGCTATT
    TCTGATGCAC GATTACACCC ATGTGGTTCT GATGAAACTG AAACAGGAGG TGATTGTGTT
    CGTCAGCTGG CTGCCTCATT GCCACCTCCA CCTGCAGGAA GGCCAGAGGT TCTGGTGGAG
    TTGATTGAGT CCAGGCTTTT CTGTAGGTGT TCTTTTGATG TTCCCGCTAC AAAAAACTCA
    GTGGGATTTC ACATAGCTTG GTCTAGGCTT TCTTCTCAAG AAGTCAAAGA GGAGCTGACA
    CAAGAGACCA CAGTTCAGGC ATTCTCTCTT TTAGAACTTG ATGGCATAAA TCTCAGACTT
    GGAGACAGGA TATTCTGCAG CGCTTCTGTC TTTTTCTTGG AGAATCCTCA TGTACAAAGT
    GTAGCCATCG AAAGCCAAGA ATTTTTTGCA GGCTTTAAGC TACAGCCTGA ATTGAGCACT
    ATATCAGAGG ATGGGAAAGA ATACTACCTG AGGATAGAAA GCACAGTTCC TATTATTTGT
    TCTGAATTTA GTGAGCTTGA TCAAGAATGC AAAATCTCAT TAAAACTGAA AACTATTGGT
    CAAGGTAGAG AGCACCTAGG CTTAAATTTG GCACTGTCTT CCTGTCATGT GGACCTTCTC
    CAGACATCTT CCTGTGCTAA TGGAACCTGT AGCCACACTT TTGTGTACTA CACTGCTGTC
    ACAGATTTTT CTCGAGATGG AGATAGAGTC TCAAACATTG TAGTGCAACC AATAGTTAAT
    GAGGATTTCC TGTGGAACAA CTACATTCCA GACAGCATCC AGATCAAAGT AAAGGATGTC
    CCAACTGCTT ACTGCTATAC ATTTACTGAC CCACATATAA TTACATTTGA TGGCAGGGTA
    TATGATAATT TCAAGACTGG AACATTTGTG CTTTATAAGA GTATGTCACG TGATTTTGAA
    GTCCATGTAC GTCAGTGGGA CTGCAGAAGC CTTCACTATC CAGTGTCATG TAATTGTGGG
    TTTGTTGCCC AGGAAGGAGG TGATATAGTT ACTTTTGATA TGTGCAATGG TCAGCTACGT
    GAATCACAAC CATATTTGTT CATAAAAAGC CAAGATGTAA CCAGGAATAT CAAGATAAGT
    GAATCTTACT TAGGAAGAAA AGTCACAATC TGGTTTTCTT CTGGGGCATT TATCCGTGCT
    GATCTTGGTG AATGGGGCAT GAGTCTAACG ATCAGAGCCC CTAGTGTAGA TTACAGAAAC
    ACTCTGGGAC TTTGTGGAAC CTTTGATGAA AATCCGGAAA ATGATTTCCA TGACAAAAAT
    GGGATGCAAA TTGATCAAAA TTTTAACAAT TATGTTGCTT TTATTAATGA ATGGAGGATT
    TTACCAGGAA AAAGCATGTC TGACACACTG CCAGTTTCTA TGACATCACC TGGAAAGCCA
    TCCTATTGTA GCTGTTCATT GGACACTGCA GCGTATCCGT CTTCCGAAGA TCTGGACAGT
    GTTTCTCGAT CAGAAATTGC CTTGGGTTGC AAAGACCTCA ATCATGTCAG CTTATCTTCT
    TTGATACCAG AACTAGATGT CACCTCCGAA TATATTAATT CAGACACTCT TGTCAGAGAG
    ATAAACAAGC ATACATCTCC AGAAGAATAT AATCTAAATT TATTTCTGCA AGAAAAAAAA
    CACATAAACC TGACTAAACT CGGCTTAAAT GTACAAAAAC ATCCTGGAAA TGAGAAAGAA
    GATTCACTAC AATATTTGGC CAATAAGAAA TATACACAAG GCCGGGGAAG CCACAGCCAA
    GAAATGAGGT ACAATCGACA AAACAGATGG AAACGGCAGA ACTTTCATGA GTTTCCTCCT
    TTGTTTGCTT TCCCGAGTCT CAGCCAAACG GATCTGGAAG AACTTACTTA TTTTTTCCCA
    GAGGACCATG CTGAGGATGT ACAGCAAGAG TTTTTCCCCT CTTGGCCCAC TCCCTCTGGC
    CTCACCGAGT ATAGCACCTT GACCCTCTGT CAGGAGACTC TAGCCAACTC CAGCATAGGA
    AGGCTGTGTC TTGCTTTTCT TGGCAAGAGA TTAGACAGTG TTATAGAGAT GTGTGTGAAG
    GATGTTCTGT TAAAAGATGA TCTTAGTTGG GCAGAAGCAG GTGTGGCCCT TTTAGAAAAT
    GAATGTGAAA AGAGGATTGT GGAGGAGGGG AAATATAACA CAGAAGAGTA TGGCACATCA
    ATTGAAGACA TTCTCTCAGT ATTAAAATGC CCCAATTTAT GCAGCGGCAA TGGGCAGTGC
    ATGGAATGGG GGTGTGCGTG TTCCCCAAGC TTTAGTTCCT ATGACTGCAG TGATTCCTAT
    GACAAAGCTC CTGAAATTAC AGAGCTTGGG AATGCTGGAT TCTGTGATGT TCAAAAATAT
    AATTGTATGA TGGTGAGAGT TTTTGGCAAA GGCTTCAAAG AATTACCTTC AATTAAATGT
    GAAGTTACTA AGCTACAGTA TAATAGCAGT GAATGGATGC CTGGAGAACC CATCTATACA
    CAGACTGTTT TCCACAATAG CAGAGCTGTT GATTGTCAGC TGCCCACTGA TGTTCAGCAG
    TTTGATACCA TGGATCTGGT GGGTGGGAAA CCAACTGGGA AGTGGCAGTT GAAGGTATCT
    AATGATGGTT ATAAATTCAG TAATCCCAAA ATAACGGTCA TATATGATGG TGCTTGCCAA
    GTTTGTGGTC TCTATAAAAA TGACTCATGT ACTATAAAGG AAAATGTTTG CATTATTGAT
    GGACTCTGCT ATGTTGAAGG AGACAAAAAT CCAACCAGCC CTTGTTTGAT TTGTAGACCC
    AAAATTTCAA GATTTACTTG GTCATTTTTA GAAAACAACC AGCCCCCAGT GATTCAAGCA
    TTGCAAGACA AATTACAGAC ATTTTATGGT GAAAACTTTG AGTATCAGTT CGTGGCCTTC
    GATCCAGAAG GTTCTGACAT CCATTTTACG TTGGACTCTG GTCCTGAAGG GGCAAGTGTT
    TCCTCTGCAG GGCTTTTTAT GTGGAAAACA GATTTACTAA CTACCCAACA AATTACTGTA
    CGCCTTAATG ATGACTGCGA TGCTGAAACT AGAGTCACGA TTGAGGTGAC TGTGAAGTCT
    TGTGATTGCT TGAATGGTGG ATCATGTGTA TCTGATAGGA ACTTTTCTCC AGGGAGTGGA
    GTGTACCTGT GTGTCTGCTT GCCTGGCTTC CATGGCAGCC TTTGTGAAGT GGACATTAGT
    GGGTGCCAAT CCAACCCTTG TGGTCTTGGC AGCTATATTA GTGGTTTTCA CAGTTATTCC
    TGTGATTGTC CACCTGAGCT CAAAGGCAAA AATTGCCAGG AAGATGTTGA TGAGTGTGAC
    TCCAAGCCTT GCTTTGAGGG AGTAGCATGC CTGAATACCT TTGGTTCCTA TCATTGTGGT
    TCATGTCCAG AAGGATTTTA TGGGGATGGA AAAATATGCC ATGTTGAAAC ACAGTTTGTA
    AATCAATTTA CTACACAAAC TGTGGTTCTC ACAAGATCTG ATAAAAGTGT AAATAAAGAA
    GAGGATGATA AAAATGCCCA AGGGAGAAAG AGGCATGTTA AGCCAACAAG TGGAAATGCC
    TTCACTATAA ATTTTCAGCC ACCATCAACA GAATATTCAC TAATTAGTAC AAGATTTACT
    CCTACTCCCG CTACTATCAA AAAGTTACTG AGCACAACTA ACAACATCTC CCGCTCATCA
    CTGAAATTAA TTACAGCCCA AATGGATAAT TCAGTAAAGC CTATTTCCCA TCAGATATCA
    GATATTGAAC ATGTGGATCG TAGTCTCAGT ACAAATCTCA GTCTGGCCAT TTGTAAATAT
    CCATGTGGAA AAAGTAGGGA GTGTGTTGCC CCAAACATCT GCAAATGTAA ACCTGGTTAC
    ATTGGTTCTA ACTGCCAAAC TGCTCTTTGT GACCCTGATT GCAAAAACCA TGGAAAATGT
    ATTAAGCCTA ACATTTGTCA GTGTCTTCCA GGACATGGTG GAGCAACCTG TGATGAAGAA
    CATTGTAACC CACCTTGTCA ACATGGTGGC ACATGCTTGG CTGGGAATCT CTGCACTTGT
    CCTTATGGTT TTGTAGGACC AAGGTGTGAA ACAATGGTTT GTAACAGGCA CTGTGAAAAT
    GGAGGCCAGT GTCTTACACC AGATATTTGC CAGTGCAAAC CTGGCTGGTA TGGACCCACC
    TGTAGTACAG CTTTGTGCGA CCCTGTCTGC CTCAATGGTG GTTCGTGTAA TAAGCCAAAT
    ACTTGCCTCT GTCCAAATGG ATTCTTTGGG GAACACTGTC AGAATGCTTT CTGTCACCCT
    CCCTGTAAGA ATGGTGGCCA CTGCATGAGA AATAATGTGT GCGTCTGTCG TGAAGGATAC
    ACTGGTAGGA GATTCCAAAA AAGCATCTGT GATCCTACGT GCATGAATGG AGGAAAATGT
    GTGGGACCAA GCACTTGCTC TTGTCCTTCT GGTTGGAGTG GGAAACGATG CAACACACCT
    ATCTGCTTGC AGAAATGTAA AAACGGTGGT GAATGCATTG CGCCCAGCAT ATGCCATTGT
    CCTTCCTCCT GGGAAGGAGT GCGGTGTCAA ATACCAATTT GCAACCCAAA ATGTCTTTAT
    GGAGGCAGAT GCATATTTCC CAATGTGTGT TCCTGCCGCA CTGAATACTC TGGAGTCAAA
    TGTGAGAAGA AAATACAGAT AAGACGCCAT TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu78061
    Clone ID Related Accession (Same CDS sequence) XM_011515175.1
    Accession Version XM_011515175.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4995bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product von Willebrand factor D and EGF domain-containing protein isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000007.14) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    ATGCCTGGCG GAGCCTGCGT GCTGGTGATC GCGCTGATGT TCCTGGCCTG GGGGGAAGCT 
    CAGGAGTGCT CTCCTGGGGG ACACCAGTTT CTTCGGAGTC CTTATAGAAG TGTCCGTTTT
    GACTCATGGC ACCTCCAGCA GTCAGCTGTT CAAGACCTAA TATGTGACCA TTCCCTCTCC
    CCTGGATGGT ATAGATTTCT CATCCTTGAC AGACCTGCCG AGATGCCAAC CAAATGTGTT
    GAGATGAACC ATTGTGGAAC TCAGGCCCCC ATCTGGCTGT CTCTGAGAGA TTCAGAAACA
    CTGCCATCTC CTGGGGAGAT CAAGCAATTG ACAGCTTGTG CAACATGGCA GTTTTTGTTC
    AGCACTACAA AAGACTGCTG TCTCTTTCAA ATCCCAGTGT CTGTAAGAAA CTGTGGGAAC
    TTTTCTGTAT ACTTACTACA ACCAACTCAG GGATGTATGG GCTACTGTGC GGAAGCTATT
    TCTGATGCAC GATTACACCC ATGTGGTTCT GATGAAACTG AAACAGGAGG TGATTGTGTT
    CGTCAGCTGG CTGCCTCATT GCCACCTCCA CCTGCAGGAA GGCCAGAGGT TCTGGTGGAG
    TTGATTGAGT CCAGGCTTTT CTGTAGGTGT TCTTTTGATG TTCCCGCTAC AAAAAACTCA
    GTGGGATTTC ACATAGCTTG GTCTAGGCTT TCTTCTCAAG AAGTCAAAGA GGAGCTGACA
    CAAGAGACCA CAGTTCAGGC ATTCTCTCTT TTAGAACTTG ATGGCATAAA TCTCAGACTT
    GGAGACAGGA TATTCTGCAG CGCTTCTGTC TTTTTCTTGG AGAATCCTCA TGTACAAAGT
    GTAGCCATCG AAAGCCAAGA ATTTTTTGCA GGCTTTAAGC TACAGCCTGA ATTGAGCACT
    ATATCAGAGG ATGGGAAAGA ATACTACCTG AGGATAGAAA GCACAGTTCC TATTATTTGT
    TCTGAATTTA GTGAGCTTGA TCAAGAATGC AAAATCTCAT TAAAACTGAA AACTATTGGT
    CAAGGTAGAG AGCACCTAGG CTTAAATTTG GCACTGTCTT CCTGTCATGT GGACCTTCTC
    CAGACATCTT CCTGTGCTAA TGGAACCTGT AGCCACACTT TTGTGTACTA CACTGCTGTC
    ACAGATTTTT CTCGAGATGG AGATAGAGTC TCAAACATTG TAGTGCAACC AATAGTTAAT
    GAGGATTTCC TGTGGAACAA CTACATTCCA GACAGCATCC AGATCAAAGT AAAGGATGTC
    CCAACTGCTT ACTGCTATAC ATTTACTGAC CCACATATAA TTACATTTGA TGGCAGGGTA
    TATGATAATT TCAAGACTGG AACATTTGTG CTTTATAAGA GTATGTCACG TGATTTTGAA
    GTCCATGTAC GTCAGTGGGA CTGCAGAAGC CTTCACTATC CAGTGTCATG TAATTGTGGG
    TTTGTTGCCC AGGAAGGAGG TGATATAGTT ACTTTTGATA TGTGCAATGG TCAGCTACGT
    GAATCACAAC CATATTTGTT CATAAAAAGC CAAGATGTAA CCAGGAATAT CAAGATAAGT
    GAATCTTACT TAGGAAGAAA AGTCACAATC TGGTTTTCTT CTGGGGCATT TATCCGTGCT
    GATCTTGGTG AATGGGGCAT GAGTCTAACG ATCAGAGCCC CTAGTGTAGA TTACAGAAAC
    ACTCTGGGAC TTTGTGGAAC CTTTGATGAA AATCCGGAAA ATGATTTCCA TGACAAAAAT
    GGGATGCAAA TTGATCAAAA TTTTAACAAT TATGTTGCTT TTATTAATGA ATGGAGGATT
    TTACCAGGAA AAAGCATGTC TGACACACTG CCAGTTTCTA TGACATCACC TGGAAAGCCA
    TCCTATTGTA GCTGTTCATT GGACACTGCA GCGTATCCGT CTTCCGAAGA TCTGGACAGT
    GTTTCTCGAT CAGAAATTGC CTTGGGTTGC AAAGACCTCA ATCATGTCAG CTTATCTTCT
    TTGATACCAG AACTAGATGT CACCTCCGAA TATATTAATT CAGACACTCT TGTCAGAGAG
    ATAAACAAGC ATACATCTCC AGAAGAATAT AATCTAAATT TATTTCTGCA AGAAAAAAAA
    CACATAAACC TGACTAAACT CGGCTTAAAT GTACAAAAAC ATCCTGGAAA TGAGAAAGAA
    GATTCACTAC AATATTTGGC CAATAAGAAA TATACACAAG GCCGGGGAAG CCACAGCCAA
    GAAATGAGGT ACAATCGACA AAACAGATGG AAACGGCAGA ACTTTCATGA GTTTCCTCCT
    TTGTTTGCTT TCCCGAGTCT CAGCCAAACG GATCTGGAAG AACTTACTTA TTTTTTCCCA
    GAGGACCATG CTGAGGATGT ACAGCAAGAG TTTTTCCCCT CTTGGCCCAC TCCCTCTGGC
    CTCACCGAGT ATAGCACCTT GACCCTCTGT CAGGAGACTC TAGCCAACTC CAGCATAGGA
    AGGCTGTGTC TTGCTTTTCT TGGCAAGAGA TTAGACAGTG TTATAGAGAT GTGTGTGAAG
    GATGTTCTGT TAAAAGATGA TCTTAGTTGG GCAGAAGCAG GTGTGGCCCT TTTAGAAAAT
    GAATGTGAAA AGAGGATTGT GGAGGAGGGG AAATATAACA CAGAAGAGTA TGGCACATCA
    ATTGAAGACA TTCTCTCAGT ATTAAAATGC CCCAATTTAT GCAGCGGCAA TGGGCAGTGC
    ATGGAATGGG GGTGTGCGTG TTCCCCAAGC TTTAGTTCCT ATGACTGCAG TGATTCCTAT
    GACAAAGCTC CTGAAATTAC AGAGCTTGGG AATGCTGGAT TCTGTGATGT TCAAAAATAT
    AATTGTATGA TGGTGAGAGT TTTTGGCAAA GGCTTCAAAG AATTACCTTC AATTAAATGT
    GAAGTTACTA AGCTACAGTA TAATAGCAGT GAATGGATGC CTGGAGAACC CATCTATACA
    CAGACTGTTT TCCACAATAG CAGAGCTGTT GATTGTCAGC TGCCCACTGA TGTTCAGCAG
    TTTGATACCA TGGATCTGGT GGGTGGGAAA CCAACTGGGA AGTGGCAGTT GAAGGTATCT
    AATGATGGTT ATAAATTCAG TAATCCCAAA ATAACGGTCA TATATGATGG TGCTTGCCAA
    GTTTGTGGTC TCTATAAAAA TGACTCATGT ACTATAAAGG AAAATGTTTG CATTATTGAT
    GGACTCTGCT ATGTTGAAGG AGACAAAAAT CCAACCAGCC CTTGTTTGAT TTGTAGACCC
    AAAATTTCAA GATTTACTTG GTCATTTTTA GAAAACAACC AGCCCCCAGT GATTCAAGCA
    TTGCAAGACA AATTACAGAC ATTTTATGGT GAAAACTTTG AGTATCAGTT CGTGGCCTTC
    GATCCAGAAG GTTCTGACAT CCATTTTACG TTGGACTCTG GTCCTGAAGG GGCAAGTGTT
    TCCTCTGCAG GGCTTTTTAT GTGGAAAACA GATTTACTAA CTACCCAACA AATTACTGTA
    CGCCTTAATG ATGACTGCGA TGCTGAAACT AGAGTCACGA TTGAGGTGAC TGTGAAGTCT
    TGTGATTGCT TGAATGGTGG ATCATGTGTA TCTGATAGGA ACTTTTCTCC AGGGAGTGGA
    GTGTACCTGT GTGTCTGCTT GCCTGGCTTC CATGGCAGCC TTTGTGAAGT GGACATTAGT
    GGGTGCCAAT CCAACCCTTG TGGTCTTGGC AGCTATATTA GTGGTTTTCA CAGTTATTCC
    TGTGATTGTC CACCTGAGCT CAAAGTTGAA ACACAGTTTG TAAATCAATT TACTACACAA
    ACTGTGGTTC TCACAAGATC TGATAAAAGT GTAAATAAAG AAGAGGATGA TAAAAATGCC
    CAAGGGAGAA AGAGGCATGT TAAGCCAACA AGTGGAAATG CCTTCACTAT AAATTTTCAG
    CCACCATCAA CAGAATATTC ACTAATTAGT ACAAGATTTA CTCCTACTCC CGCTACTATC
    AAAAAGTTAC TGAGCACAAC TAACAACATC TCCCGCTCAT CACTGAAATT AATTACAGCC
    CAAATGGATA ATTCAGTAAA GCCTATTTCC CATCAGATAT CAGATATTGA ACATGTGGAT
    CGTAGTCTCA GTACAAATCT CAGTCTGGCC ATTTGTAAAT ATCCATGTGG AAAAAGTAGG
    GAGTGTGTTG CCCCAAACAT CTGCAAATGT AAACCTGGTT ACATTGGTTC TAACTGCCAA
    ACTGCTCTTT GTGACCCTGA TTGCAAAAAC CATGGAAAAT GTATTAAGCC TAACATTTGT
    CAGTGTCTTC CAGGACATGG TGGAGCAACC TGTGATGAAG AACATTGTAA CCCACCTTGT
    CAACATGGTG GCACATGCTT GGCTGGGAAT CTCTGCACTT GTCCTTATGG TTTTGTAGGA
    CCAAGGTGTG AAACAATGGT TTGTAACAGG CACTGTGAAA ATGGAGGCCA GTGTCTTACA
    CCAGATATTT GCCAGTGCAA ACCTGGCTGG TATGGACCCA CCTGTAGTAC AGCTTTGTGC
    GACCCTGTCT GCCTCAATGG TGGTTCGTGT AATAAGCCAA ATACTTGCCT CTGTCCAAAT
    GGATTCTTTG GGGAACACTG TCAGAATGCT TTCTGTCACC CTCCCTGTAA GAATGGTGGC
    CACTGCATGA GAAATAATGT GTGCGTCTGT CGTGAAGGAT ACACTGGTAG GAGATTCCAA
    AAAAGCATCT GTGATCCTAC GTGCATGAAT GGAGGAAAAT GTGTGGGACC AAGCACTTGC
    TCTTGTCCTT CTGGTTGGAG TGGGAAACGA TGCAACACAC CTATCTGCTT GCAGAAATGT
    AAAAACGGTG GTGAATGCAT TGCGCCCAGC ATATGCCATT GTCCTTCCTC CTGGGAAGGA
    GTGCGGTGTC AAATACCAAT TTGCAACCCA AAATGTCTTT ATGGAGGCAG ATGCATATTT
    CCCAATGTGT GTTCCTGCCG CACTGAATAC TCTGGAGTCA AATGTGAGAA GAAAATACAG
    ATAAGACGCC ATTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_011513477.1
    CDS188..5182
    Translation

    Target ORF information:

    RefSeq Version XM_011515175.1
    Organism Homo sapiens(human)
    Definition Homo sapiens von Willebrand factor D and EGF domains (VWDE), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_011515175.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    ATGCCTGGCG GAGCCTGCGT GCTGGTGATC GCGCTGATGT TCCTGGCCTG GGGGGAAGCT 
    CAGGAGTGCT CTCCTGGGGG ACACCAGTTT CTTCGGAGTC CTTATAGAAG TGTCCGTTTT
    GACTCATGGC ACCTCCAGCA GTCAGCTGTT CAAGACCTAA TATGTGACCA TTCCCTCTCC
    CCTGGATGGT ATAGATTTCT CATCCTTGAC AGACCTGCCG AGATGCCAAC CAAATGTGTT
    GAGATGAACC ATTGTGGAAC TCAGGCCCCC ATCTGGCTGT CTCTGAGAGA TTCAGAAACA
    CTGCCATCTC CTGGGGAGAT CAAGCAATTG ACAGCTTGTG CAACATGGCA GTTTTTGTTC
    AGCACTACAA AAGACTGCTG TCTCTTTCAA ATCCCAGTGT CTGTAAGAAA CTGTGGGAAC
    TTTTCTGTAT ACTTACTACA ACCAACTCAG GGATGTATGG GCTACTGTGC GGAAGCTATT
    TCTGATGCAC GATTACACCC ATGTGGTTCT GATGAAACTG AAACAGGAGG TGATTGTGTT
    CGTCAGCTGG CTGCCTCATT GCCACCTCCA CCTGCAGGAA GGCCAGAGGT TCTGGTGGAG
    TTGATTGAGT CCAGGCTTTT CTGTAGGTGT TCTTTTGATG TTCCCGCTAC AAAAAACTCA
    GTGGGATTTC ACATAGCTTG GTCTAGGCTT TCTTCTCAAG AAGTCAAAGA GGAGCTGACA
    CAAGAGACCA CAGTTCAGGC ATTCTCTCTT TTAGAACTTG ATGGCATAAA TCTCAGACTT
    GGAGACAGGA TATTCTGCAG CGCTTCTGTC TTTTTCTTGG AGAATCCTCA TGTACAAAGT
    GTAGCCATCG AAAGCCAAGA ATTTTTTGCA GGCTTTAAGC TACAGCCTGA ATTGAGCACT
    ATATCAGAGG ATGGGAAAGA ATACTACCTG AGGATAGAAA GCACAGTTCC TATTATTTGT
    TCTGAATTTA GTGAGCTTGA TCAAGAATGC AAAATCTCAT TAAAACTGAA AACTATTGGT
    CAAGGTAGAG AGCACCTAGG CTTAAATTTG GCACTGTCTT CCTGTCATGT GGACCTTCTC
    CAGACATCTT CCTGTGCTAA TGGAACCTGT AGCCACACTT TTGTGTACTA CACTGCTGTC
    ACAGATTTTT CTCGAGATGG AGATAGAGTC TCAAACATTG TAGTGCAACC AATAGTTAAT
    GAGGATTTCC TGTGGAACAA CTACATTCCA GACAGCATCC AGATCAAAGT AAAGGATGTC
    CCAACTGCTT ACTGCTATAC ATTTACTGAC CCACATATAA TTACATTTGA TGGCAGGGTA
    TATGATAATT TCAAGACTGG AACATTTGTG CTTTATAAGA GTATGTCACG TGATTTTGAA
    GTCCATGTAC GTCAGTGGGA CTGCAGAAGC CTTCACTATC CAGTGTCATG TAATTGTGGG
    TTTGTTGCCC AGGAAGGAGG TGATATAGTT ACTTTTGATA TGTGCAATGG TCAGCTACGT
    GAATCACAAC CATATTTGTT CATAAAAAGC CAAGATGTAA CCAGGAATAT CAAGATAAGT
    GAATCTTACT TAGGAAGAAA AGTCACAATC TGGTTTTCTT CTGGGGCATT TATCCGTGCT
    GATCTTGGTG AATGGGGCAT GAGTCTAACG ATCAGAGCCC CTAGTGTAGA TTACAGAAAC
    ACTCTGGGAC TTTGTGGAAC CTTTGATGAA AATCCGGAAA ATGATTTCCA TGACAAAAAT
    GGGATGCAAA TTGATCAAAA TTTTAACAAT TATGTTGCTT TTATTAATGA ATGGAGGATT
    TTACCAGGAA AAAGCATGTC TGACACACTG CCAGTTTCTA TGACATCACC TGGAAAGCCA
    TCCTATTGTA GCTGTTCATT GGACACTGCA GCGTATCCGT CTTCCGAAGA TCTGGACAGT
    GTTTCTCGAT CAGAAATTGC CTTGGGTTGC AAAGACCTCA ATCATGTCAG CTTATCTTCT
    TTGATACCAG AACTAGATGT CACCTCCGAA TATATTAATT CAGACACTCT TGTCAGAGAG
    ATAAACAAGC ATACATCTCC AGAAGAATAT AATCTAAATT TATTTCTGCA AGAAAAAAAA
    CACATAAACC TGACTAAACT CGGCTTAAAT GTACAAAAAC ATCCTGGAAA TGAGAAAGAA
    GATTCACTAC AATATTTGGC CAATAAGAAA TATACACAAG GCCGGGGAAG CCACAGCCAA
    GAAATGAGGT ACAATCGACA AAACAGATGG AAACGGCAGA ACTTTCATGA GTTTCCTCCT
    TTGTTTGCTT TCCCGAGTCT CAGCCAAACG GATCTGGAAG AACTTACTTA TTTTTTCCCA
    GAGGACCATG CTGAGGATGT ACAGCAAGAG TTTTTCCCCT CTTGGCCCAC TCCCTCTGGC
    CTCACCGAGT ATAGCACCTT GACCCTCTGT CAGGAGACTC TAGCCAACTC CAGCATAGGA
    AGGCTGTGTC TTGCTTTTCT TGGCAAGAGA TTAGACAGTG TTATAGAGAT GTGTGTGAAG
    GATGTTCTGT TAAAAGATGA TCTTAGTTGG GCAGAAGCAG GTGTGGCCCT TTTAGAAAAT
    GAATGTGAAA AGAGGATTGT GGAGGAGGGG AAATATAACA CAGAAGAGTA TGGCACATCA
    ATTGAAGACA TTCTCTCAGT ATTAAAATGC CCCAATTTAT GCAGCGGCAA TGGGCAGTGC
    ATGGAATGGG GGTGTGCGTG TTCCCCAAGC TTTAGTTCCT ATGACTGCAG TGATTCCTAT
    GACAAAGCTC CTGAAATTAC AGAGCTTGGG AATGCTGGAT TCTGTGATGT TCAAAAATAT
    AATTGTATGA TGGTGAGAGT TTTTGGCAAA GGCTTCAAAG AATTACCTTC AATTAAATGT
    GAAGTTACTA AGCTACAGTA TAATAGCAGT GAATGGATGC CTGGAGAACC CATCTATACA
    CAGACTGTTT TCCACAATAG CAGAGCTGTT GATTGTCAGC TGCCCACTGA TGTTCAGCAG
    TTTGATACCA TGGATCTGGT GGGTGGGAAA CCAACTGGGA AGTGGCAGTT GAAGGTATCT
    AATGATGGTT ATAAATTCAG TAATCCCAAA ATAACGGTCA TATATGATGG TGCTTGCCAA
    GTTTGTGGTC TCTATAAAAA TGACTCATGT ACTATAAAGG AAAATGTTTG CATTATTGAT
    GGACTCTGCT ATGTTGAAGG AGACAAAAAT CCAACCAGCC CTTGTTTGAT TTGTAGACCC
    AAAATTTCAA GATTTACTTG GTCATTTTTA GAAAACAACC AGCCCCCAGT GATTCAAGCA
    TTGCAAGACA AATTACAGAC ATTTTATGGT GAAAACTTTG AGTATCAGTT CGTGGCCTTC
    GATCCAGAAG GTTCTGACAT CCATTTTACG TTGGACTCTG GTCCTGAAGG GGCAAGTGTT
    TCCTCTGCAG GGCTTTTTAT GTGGAAAACA GATTTACTAA CTACCCAACA AATTACTGTA
    CGCCTTAATG ATGACTGCGA TGCTGAAACT AGAGTCACGA TTGAGGTGAC TGTGAAGTCT
    TGTGATTGCT TGAATGGTGG ATCATGTGTA TCTGATAGGA ACTTTTCTCC AGGGAGTGGA
    GTGTACCTGT GTGTCTGCTT GCCTGGCTTC CATGGCAGCC TTTGTGAAGT GGACATTAGT
    GGGTGCCAAT CCAACCCTTG TGGTCTTGGC AGCTATATTA GTGGTTTTCA CAGTTATTCC
    TGTGATTGTC CACCTGAGCT CAAAGTTGAA ACACAGTTTG TAAATCAATT TACTACACAA
    ACTGTGGTTC TCACAAGATC TGATAAAAGT GTAAATAAAG AAGAGGATGA TAAAAATGCC
    CAAGGGAGAA AGAGGCATGT TAAGCCAACA AGTGGAAATG CCTTCACTAT AAATTTTCAG
    CCACCATCAA CAGAATATTC ACTAATTAGT ACAAGATTTA CTCCTACTCC CGCTACTATC
    AAAAAGTTAC TGAGCACAAC TAACAACATC TCCCGCTCAT CACTGAAATT AATTACAGCC
    CAAATGGATA ATTCAGTAAA GCCTATTTCC CATCAGATAT CAGATATTGA ACATGTGGAT
    CGTAGTCTCA GTACAAATCT CAGTCTGGCC ATTTGTAAAT ATCCATGTGG AAAAAGTAGG
    GAGTGTGTTG CCCCAAACAT CTGCAAATGT AAACCTGGTT ACATTGGTTC TAACTGCCAA
    ACTGCTCTTT GTGACCCTGA TTGCAAAAAC CATGGAAAAT GTATTAAGCC TAACATTTGT
    CAGTGTCTTC CAGGACATGG TGGAGCAACC TGTGATGAAG AACATTGTAA CCCACCTTGT
    CAACATGGTG GCACATGCTT GGCTGGGAAT CTCTGCACTT GTCCTTATGG TTTTGTAGGA
    CCAAGGTGTG AAACAATGGT TTGTAACAGG CACTGTGAAA ATGGAGGCCA GTGTCTTACA
    CCAGATATTT GCCAGTGCAA ACCTGGCTGG TATGGACCCA CCTGTAGTAC AGCTTTGTGC
    GACCCTGTCT GCCTCAATGG TGGTTCGTGT AATAAGCCAA ATACTTGCCT CTGTCCAAAT
    GGATTCTTTG GGGAACACTG TCAGAATGCT TTCTGTCACC CTCCCTGTAA GAATGGTGGC
    CACTGCATGA GAAATAATGT GTGCGTCTGT CGTGAAGGAT ACACTGGTAG GAGATTCCAA
    AAAAGCATCT GTGATCCTAC GTGCATGAAT GGAGGAAAAT GTGTGGGACC AAGCACTTGC
    TCTTGTCCTT CTGGTTGGAG TGGGAAACGA TGCAACACAC CTATCTGCTT GCAGAAATGT
    AAAAACGGTG GTGAATGCAT TGCGCCCAGC ATATGCCATT GTCCTTCCTC CTGGGAAGGA
    GTGCGGTGTC AAATACCAAT TTGCAACCCA AAATGTCTTT ATGGAGGCAG ATGCATATTT
    CCCAATGTGT GTTCCTGCCG CACTGAATAC TCTGGAGTCA AATGTGAGAA GAAAATACAG
    ATAAGACGCC ATTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

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