ARHGEF12 cDNA ORF clone, Homo sapiens(human)

  • Gene

  • Clones

  • gRNAs

The following ARHGEF12 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ARHGEF12 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

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***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OHu21676 NM_015313.3
Latest version!
Homo sapiens Rho guanine nucleotide exchange factor 12 (ARHGEF12), transcript variant 1, mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK
$579.50-$811.30
$1159.00
OHu92069 XM_017017420.1
Latest version!
Homo sapiens Rho guanine nucleotide exchange factor 12 (ARHGEF12), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $727.30
$1039.00
OHu92070 XM_017017422.2
Latest version!
Homo sapiens Rho guanine nucleotide exchange factor 12 (ARHGEF12), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $727.30
$1039.00
OHu07698 NM_001198665.2
Latest version!
Homo sapiens Rho guanine nucleotide exchange factor 12 (ARHGEF12), transcript variant 2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $811.30
$1159.00
OHu45709 NM_001301084.2
Latest version!
Homo sapiens Rho guanine nucleotide exchange factor 12 (ARHGEF12), transcript variant 3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $727.30
$1039.00
OHu45707 XM_006718805.3
Latest version!
Homo sapiens Rho guanine nucleotide exchange factor 12 (ARHGEF12), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $811.30
$1159.00
OHu45709 XM_011542720.2
Latest version!
Homo sapiens Rho guanine nucleotide exchange factor 12 (ARHGEF12), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $727.30
$1039.00
OHu45709 XM_017017421.1
Latest version!
Homo sapiens Rho guanine nucleotide exchange factor 12 (ARHGEF12), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $727.30
$1039.00
View Old Accession Versions >>
OHu21676 NM_015313.2 Homo sapiens Rho guanine nucleotide exchange factor 12 (ARHGEF12), transcript variant 1, mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK
$579.50-$811.30
$1159.00
OHu92070 XM_017017422.1 Homo sapiens Rho guanine nucleotide exchange factor 12 (ARHGEF12), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $727.30
$1039.00
OHu07698 NM_001198665.1 Homo sapiens Rho guanine nucleotide exchange factor 12 (ARHGEF12), transcript variant 2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $811.30
$1159.00
OHu45709 NM_001301084.1 Homo sapiens Rho guanine nucleotide exchange factor 12 (ARHGEF12), transcript variant 3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $727.30
$1039.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OHu21676
    Clone ID Related Accession (Same CDS sequence) NM_015313.3 , NM_015313.2
    Accession Version NM_015313.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4635bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-10-23
    Organism Homo sapiens(human)
    Product rho guanine nucleotide exchange factor 12 isoform 1
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AP001150.5, BC063117.1, AF180681.1, AK304316.1 and AP000758.5. This sequence is a reference standard in the RefSeqGene project. On Nov 22, 2018 this sequence version replaced NM_015313.2. Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF180681.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000397843.7/ ENSP00000380942.2 RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: full length.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    ATGAGTGGCA CACAGTCTAC TATCACCGAC AGGTTTCCCC TCAAAAAACC TATAAGGCAT 
    GGAAGTATTT TGAACCGAGA GTCACCAACA GATAAGAAGC AGAAAGTTGA GCGCATTGCA
    TCACATGATT TTGACCCCAC AGATAGCTCC TCCAAGAAGA CAAAGTCTAG TTCAGAGGAG
    AGTAGATCCG AGATATATGG TCTTGTTCAG CGTTGCGTAA TCATCCAGAA AGATGACAAT
    GGATTTGGGC TGACGGTCAG TGGAGACAAT CCAGTCTTCG TACAGTCTGT CAAAGAAGAT
    GGAGCAGCCA TGCGGGCTGG AGTACAGACA GGTGATCGAA TCATCAAGGT GAATGGAACT
    CTGGTGACTC ATTCAAATCA TCTGGAGGTG GTGAAGCTAA TCAAATCTGG TTCCTATGTA
    GCTCTCACTG TTCAGGGACG CCCACCTGGG TCGCCCCAGA TTCCACTTGC CGACTCTGAA
    GTAGAGCCGT CAGTCATTGG ACATATGTCT CCCATCATGA CATCTCCTCA TTCACCTGGA
    GCATCTGGGA ATATGGAGAG AATCACTAGT CCTGTGCTCA TGGGGGAGGA AAACAATGTG
    GTTCATAACC AGAAAGTAGA AATTCTGAGA AAAATGTTAC AGAAAGAACA GGAACGGCTA
    CAGTTATTGC AGGAAGATTA CAACCGAACA CCTGCCCAAA GATTGCTAAA AGAGATCCAA
    GAGGCCAAGA AACACATTCC TCAGCTGCAA GAGCAGTTAT CCAAAGCCAC AGGCTCTGCT
    CAGGATGGAG CTGTAGTTAC ACCCTCCAGA CCTTTAGGGG ACACCCTAAC AGTCAGTGAG
    GCAGAAACAG ATCCTGGAGA TGTACTGGGC AGGACTGACT GTAGCAGTGG AGATGCTTCT
    CGGCCCAGTA GTGACAATGC AGATAGTCCC AAGAGTGGCC CAAAAGAGAG AATTTATCTA
    GAGGAAAACC CAGAGAAAAG TGAAACAATT CAGGACACTG ACACTCAATC ACTTGTCGGA
    AGTCCCTCAA CCCGTATAGC ACCTCATATT ATTGGAGCAG AAGATGATGA TTTTGGTACT
    GAACATGAAC AGATCAATGG ACAGTGCAGC TGTTTCCAGA GCATTGAATT ACTAAAATCT
    CGCCCGGCTC ATTTGGCTGT TTTCTTACAC CATGTAGTTT CACAATTTGA CCCTGCGACT
    TTGCTCTGTT ATCTCTATTC AGACCTGTAT AAACATACCA ATTCCAAAGA AACTCGTCGC
    ATCTTCCTTG AGTTTCATCA GTTCTTTCTA GATCGATCAG CACACCTGAA AGTTTCTGTT
    CCTGATGAAA TGTCTGCAGA TCTAGAAAAG AGAAGACCTG AGCTCATTCC TGAGGATCTG
    CATCGCCACT ATATCCAAAC TATGCAAGAA AGAGTCCATC CAGAAGTTCA AAGGCACTTA
    GAAGATTTTC GGCAGAAACG TAGTATGGGA CTGACCTTGG CTGAAAGCGA GCTGACTAAA
    CTTGATGCAG AGCGAGACAA GGACCGATTG ACTTTGGAGA AGGAGCGGAC ATGTGCAGAA
    CAGATTGTTG CCAAAATTGA AGAAGTATTG ATGACTGCTC AGGCTGTAGA GGAAGATAAG
    AGCTCCACCA TGCAGTATGT TATTCTCATG TATATGAAGC ATTTGGGAGT AAAAGTGAAA
    GAGCCTCGAA ATTTGGAGCA CAAACGGGGT CGGATTGGAT TTCTTCCCAA AATCAAGCAA
    AGTATGAAGA AAGATAAAGA AGGGGAAGAA AAAGGGAAGC GAAGAGGATT CCCCAGCATC
    CTGGGACCCC CACGGAGACC AAGCCGTCAT GACAACAGTG CAATTGGCAG AGCCATGGAA
    CTACAGAAGG CGCGCCACCC TAAGCACTTA TCCACACCCT CATCTGTGAG TCCTGAACCT
    CAGGACTCTG CCAAGTTGCG CCAGAGTGGG TTAGCAAATG AAGGAACAGA CGCTGGATAC
    CTGCCTGCCA ATTCCATGTC TTCTGTAGCT TCAGGGGCCT CTTTTTCCCA GGAAGGAGGG
    AAAGAGAATG ATACAGGATC AAAGCAAGTT GGAGAAACAT CAGCACCTGG AGACACCTTA
    GATGGCACAC CTCGTACTCT CAATACTGTC TTTGATTTCC CACCACCTCC ATTAGACCAA
    GTGCAGGAGG AGGAATGTGA AGTAGAAAGG GTGACTGAAC ATGGGACACC AAAGCCCTTT
    CGAAAGTTTG ACAGTGTAGC TTTTGGAGAA AGTCAAAGTG AGGATGAACA ATTTGAAAAT
    GACTTAGAGA CAGATCCACC CAACTGGCAG CAGCTTGTTA GTCGAGAAGT GTTACTGGGA
    CTAAAACCTT GTGAAATCAA AAGACAGGAA GTGATTAATG AATTGTTCTA CACTGAAAGA
    GCTCATGTTC GAACACTGAA GGTTCTTGAT CAAGTGTTCT ATCAGCGAGT ATCCAGAGAA
    GGAATTCTGT CACCCTCAGA GCTACGGAAA ATTTTTTCAA ACTTGGAAGA TATTCTTCAA
    CTTCATATTG GATTGAATGA ACAAATGAAG GCTGTTCGAA AGAGAAATGA GACCTCTGTT
    ATCGATCAGA TTGGGGAAGA TTTGCTGACA TGGTTCAGCG GACCAGGAGA GGAGAAATTG
    AAACATGCTG CTGCTACCTT TTGCAGTAAC CAACCTTTCG CCCTGGAAAT GATCAAATCT
    CGTCAGAAAA AGGATTCTCG ATTTCAGACT TTTGTGCAAG ATGCTGAAAG TAATCCACTG
    TGTCGTCGTC TTCAACTGAA GGATATTATT CCCACTCAAA TGCAAAGGCT TACTAAGTAC
    CCACTTCTGT TGGATAATAT TGCCAAATAC ACAGAATGGC CAACAGAAAG GGAGAAGGTG
    AAGAAAGCTG CAGATCACTG TCGTCAGATC TTAAATTATG TAAATCAGGC TGTCAAGGAG
    GCAGAAAACA AGCAGCGCCT AGAAGATTAT CAGCGTCGCC TTGATACCTC CAGCCTGAAG
    TTGTCAGAGT ACCCAAATGT TGAAGAGCTC AGGAATTTGG ATTTAACAAA AAGGAAGATG
    ATTCATGAAG GGCCATTGGT TTGGAAGGTG AATAGAGATA AAACTATTGA TTTATACACG
    TTGCTGCTGG AAGACATTCT TGTATTGTTA CAAAAGCAGG ATGATAGACT GGTTTTAAGG
    TGTCATAGTA AGATTCTGGC ATCTACAGCT GATAGCAAAC ACACGTTTAG CCCTGTCATT
    AAGTTGAGTA CAGTGTTGGT TCGACAAGTG GCAACAGATA ACAAAGCTTT ATTCGTCATT
    TCCATGTCAG ACAATGGCGC TCAGATTTAT GAACTGGTGG CACAGACAGT TTCTGAAAAG
    ACTGTCTGGC AGGACCTAAT CTGTCGGATG GCTGCATCAG TGAAGGAGCA ATCCACAAAG
    CCAATTCCAT TACCACAGTC AACACCTGGC GAAGGAGATA ATGATGAAGA AGATCCTTCA
    AAATTAAAAG AGGAGCAGCA TGGCATTTCA GTCACTGGTT TGCAGAGTCC AGACAGAGAT
    TTGGGATTAG AATCTACCTT AATATCGTCA AAACCTCAGT CTCATTCACT GAGTACCTCT
    GGGAAATCAG AGGTACGTGA TCTGTTTGTG GCTGAGAGAC AGTTTGCAAA GGAACAACAT
    ACAGATGGGA CACTAAAGGA AGTTGGAGAA GATTATCAAA TCGCAATCCC AGATTCACAC
    CTGCCTGTCT CAGAAGAACG GTGGGCATTG GATGCACTAA GAAATTTGGG TTTGTTGAAG
    CAGTTGCTGG TGCAACAGCT AGGTTTGACT GAGAAGAGCG TTCAGGAAGA CTGGCAACAT
    TTCCCAAGAT ACAGAACAGC CTCTCAGGGG CCGCAGACAG ACAGTGTCAT CCAGAACTCT
    GAAAATATTA AGGCCTATCA TTCTGGTGAA GGACATATGC CCTTTAGAAC TGGAACTGGT
    GACATTGCAA CTTGTTACAG TCCACGGACT TCAACTGAAT CTTTTGCTCC ACGGGATTCA
    GTGGGACTGG CACCCCAGGA TAGCCAGGCA AGTAACATTT TAGTAATGGA CCACATGATT
    ATGACCCCAG AGATGCCTAC CATGGAGCCA GAAGGGGGTC TTGATGACAG TGGAGAGCAC
    TTTTTTGATG CCCGTGAAGC ACATAGTGAT GAGAATCCAT CAGAAGGTGA TGGAGCAGTT
    AACAAGGAAG AGAAGGATGT TAATTTACGC ATCTCAGGAA ACTATTTGAT CCTTGATGGC
    TATGACCCAG TGCAGGAGAG TTCCACAGAT GAGGAGGTTG CTTCCTCACT TACCCTGCAG
    CCCATGACAG GCATCCCTGC TGTGGAATCC ACCCACCAGC AGCAACATTC TCCTCAGAAT
    ACTCACTCCG ATGGGGCAAT TTCACCATTC ACCCCCGAAT TTCTGGTCCA GCAGCGCTGG
    GGAGCTATGG AGTATTCCTG TTTTGAGATC CAGAGTCCCT CCTCTTGTGC AGATTCACAG
    AGCCAGATCA TGGAGTACAT TCATAAGATA GAGGCTGACC TTGAACACTT AAAGAAGGTG
    GAGGAAAGTT ACACCATTCT TTGCCAAAGG CTGGCTGGAT CAGCCCTCAC AGACAAGCAC
    TCAGATAAAA GTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_056128.1
    CDS832..5466
    Translation

    Target ORF information:

    RefSeq Version NM_015313.3
    Organism Homo sapiens(human)
    Definition Homo sapiens Rho guanine nucleotide exchange factor 12 (ARHGEF12), transcript variant 1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_015313.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    ATGAGTGGCA CACAGTCTAC TATCACCGAC AGGTTTCCCC TCAAAAAACC TATAAGGCAT 
    GGAAGTATTT TGAACCGAGA GTCACCAACA GATAAGAAGC AGAAAGTTGA GCGCATTGCA
    TCACATGATT TTGACCCCAC AGATAGCTCC TCCAAGAAGA CAAAGTCTAG TTCAGAGGAG
    AGTAGATCCG AGATATATGG TCTTGTTCAG CGTTGCGTAA TCATCCAGAA AGATGACAAT
    GGATTTGGGC TGACGGTCAG TGGAGACAAT CCAGTCTTCG TACAGTCTGT CAAAGAAGAT
    GGAGCAGCCA TGCGGGCTGG AGTACAGACA GGTGATCGAA TCATCAAGGT GAATGGAACT
    CTGGTGACTC ATTCAAATCA TCTGGAGGTG GTGAAGCTAA TCAAATCTGG TTCCTATGTA
    GCTCTCACTG TTCAGGGACG CCCACCTGGG TCGCCCCAGA TTCCACTTGC CGACTCTGAA
    GTAGAGCCGT CAGTCATTGG ACATATGTCT CCCATCATGA CATCTCCTCA TTCACCTGGA
    GCATCTGGGA ATATGGAGAG AATCACTAGT CCTGTGCTCA TGGGGGAGGA AAACAATGTG
    GTTCATAACC AGAAAGTAGA AATTCTGAGA AAAATGTTAC AGAAAGAACA GGAACGGCTA
    CAGTTATTGC AGGAAGATTA CAACCGAACA CCTGCCCAAA GATTGCTAAA AGAGATCCAA
    GAGGCCAAGA AACACATTCC TCAGCTGCAA GAGCAGTTAT CCAAAGCCAC AGGCTCTGCT
    CAGGATGGAG CTGTAGTTAC ACCCTCCAGA CCTTTAGGGG ACACCCTAAC AGTCAGTGAG
    GCAGAAACAG ATCCTGGAGA TGTACTGGGC AGGACTGACT GTAGCAGTGG AGATGCTTCT
    CGGCCCAGTA GTGACAATGC AGATAGTCCC AAGAGTGGCC CAAAAGAGAG AATTTATCTA
    GAGGAAAACC CAGAGAAAAG TGAAACAATT CAGGACACTG ACACTCAATC ACTTGTCGGA
    AGTCCCTCAA CCCGTATAGC ACCTCATATT ATTGGAGCAG AAGATGATGA TTTTGGTACT
    GAACATGAAC AGATCAATGG ACAGTGCAGC TGTTTCCAGA GCATTGAATT ACTAAAATCT
    CGCCCGGCTC ATTTGGCTGT TTTCTTACAC CATGTAGTTT CACAATTTGA CCCTGCGACT
    TTGCTCTGTT ATCTCTATTC AGACCTGTAT AAACATACCA ATTCCAAAGA AACTCGTCGC
    ATCTTCCTTG AGTTTCATCA GTTCTTTCTA GATCGATCAG CACACCTGAA AGTTTCTGTT
    CCTGATGAAA TGTCTGCAGA TCTAGAAAAG AGAAGACCTG AGCTCATTCC TGAGGATCTG
    CATCGCCACT ATATCCAAAC TATGCAAGAA AGAGTCCATC CAGAAGTTCA AAGGCACTTA
    GAAGATTTTC GGCAGAAACG TAGTATGGGA CTGACCTTGG CTGAAAGCGA GCTGACTAAA
    CTTGATGCAG AGCGAGACAA GGACCGATTG ACTTTGGAGA AGGAGCGGAC ATGTGCAGAA
    CAGATTGTTG CCAAAATTGA AGAAGTATTG ATGACTGCTC AGGCTGTAGA GGAAGATAAG
    AGCTCCACCA TGCAGTATGT TATTCTCATG TATATGAAGC ATTTGGGAGT AAAAGTGAAA
    GAGCCTCGAA ATTTGGAGCA CAAACGGGGT CGGATTGGAT TTCTTCCCAA AATCAAGCAA
    AGTATGAAGA AAGATAAAGA AGGGGAAGAA AAAGGGAAGC GAAGAGGATT CCCCAGCATC
    CTGGGACCCC CACGGAGACC AAGCCGTCAT GACAACAGTG CAATTGGCAG AGCCATGGAA
    CTACAGAAGG CGCGCCACCC TAAGCACTTA TCCACACCCT CATCTGTGAG TCCTGAACCT
    CAGGACTCTG CCAAGTTGCG CCAGAGTGGG TTAGCAAATG AAGGAACAGA CGCTGGATAC
    CTGCCTGCCA ATTCCATGTC TTCTGTAGCT TCAGGGGCCT CTTTTTCCCA GGAAGGAGGG
    AAAGAGAATG ATACAGGATC AAAGCAAGTT GGAGAAACAT CAGCACCTGG AGACACCTTA
    GATGGCACAC CTCGTACTCT CAATACTGTC TTTGATTTCC CACCACCTCC ATTAGACCAA
    GTGCAGGAGG AGGAATGTGA AGTAGAAAGG GTGACTGAAC ATGGGACACC AAAGCCCTTT
    CGAAAGTTTG ACAGTGTAGC TTTTGGAGAA AGTCAAAGTG AGGATGAACA ATTTGAAAAT
    GACTTAGAGA CAGATCCACC CAACTGGCAG CAGCTTGTTA GTCGAGAAGT GTTACTGGGA
    CTAAAACCTT GTGAAATCAA AAGACAGGAA GTGATTAATG AATTGTTCTA CACTGAAAGA
    GCTCATGTTC GAACACTGAA GGTTCTTGAT CAAGTGTTCT ATCAGCGAGT ATCCAGAGAA
    GGAATTCTGT CACCCTCAGA GCTACGGAAA ATTTTTTCAA ACTTGGAAGA TATTCTTCAA
    CTTCATATTG GATTGAATGA ACAAATGAAG GCTGTTCGAA AGAGAAATGA GACCTCTGTT
    ATCGATCAGA TTGGGGAAGA TTTGCTGACA TGGTTCAGCG GACCAGGAGA GGAGAAATTG
    AAACATGCTG CTGCTACCTT TTGCAGTAAC CAACCTTTCG CCCTGGAAAT GATCAAATCT
    CGTCAGAAAA AGGATTCTCG ATTTCAGACT TTTGTGCAAG ATGCTGAAAG TAATCCACTG
    TGTCGTCGTC TTCAACTGAA GGATATTATT CCCACTCAAA TGCAAAGGCT TACTAAGTAC
    CCACTTCTGT TGGATAATAT TGCCAAATAC ACAGAATGGC CAACAGAAAG GGAGAAGGTG
    AAGAAAGCTG CAGATCACTG TCGTCAGATC TTAAATTATG TAAATCAGGC TGTCAAGGAG
    GCAGAAAACA AGCAGCGCCT AGAAGATTAT CAGCGTCGCC TTGATACCTC CAGCCTGAAG
    TTGTCAGAGT ACCCAAATGT TGAAGAGCTC AGGAATTTGG ATTTAACAAA AAGGAAGATG
    ATTCATGAAG GGCCATTGGT TTGGAAGGTG AATAGAGATA AAACTATTGA TTTATACACG
    TTGCTGCTGG AAGACATTCT TGTATTGTTA CAAAAGCAGG ATGATAGACT GGTTTTAAGG
    TGTCATAGTA AGATTCTGGC ATCTACAGCT GATAGCAAAC ACACGTTTAG CCCTGTCATT
    AAGTTGAGTA CAGTGTTGGT TCGACAAGTG GCAACAGATA ACAAAGCTTT ATTCGTCATT
    TCCATGTCAG ACAATGGCGC TCAGATTTAT GAACTGGTGG CACAGACAGT TTCTGAAAAG
    ACTGTCTGGC AGGACCTAAT CTGTCGGATG GCTGCATCAG TGAAGGAGCA ATCCACAAAG
    CCAATTCCAT TACCACAGTC AACACCTGGC GAAGGAGATA ATGATGAAGA AGATCCTTCA
    AAATTAAAAG AGGAGCAGCA TGGCATTTCA GTCACTGGTT TGCAGAGTCC AGACAGAGAT
    TTGGGATTAG AATCTACCTT AATATCGTCA AAACCTCAGT CTCATTCACT GAGTACCTCT
    GGGAAATCAG AGGTACGTGA TCTGTTTGTG GCTGAGAGAC AGTTTGCAAA GGAACAACAT
    ACAGATGGGA CACTAAAGGA AGTTGGAGAA GATTATCAAA TCGCAATCCC AGATTCACAC
    CTGCCTGTCT CAGAAGAACG GTGGGCATTG GATGCACTAA GAAATTTGGG TTTGTTGAAG
    CAGTTGCTGG TGCAACAGCT AGGTTTGACT GAGAAGAGCG TTCAGGAAGA CTGGCAACAT
    TTCCCAAGAT ACAGAACAGC CTCTCAGGGG CCGCAGACAG ACAGTGTCAT CCAGAACTCT
    GAAAATATTA AGGCCTATCA TTCTGGTGAA GGACATATGC CCTTTAGAAC TGGAACTGGT
    GACATTGCAA CTTGTTACAG TCCACGGACT TCAACTGAAT CTTTTGCTCC ACGGGATTCA
    GTGGGACTGG CACCCCAGGA TAGCCAGGCA AGTAACATTT TAGTAATGGA CCACATGATT
    ATGACCCCAG AGATGCCTAC CATGGAGCCA GAAGGGGGTC TTGATGACAG TGGAGAGCAC
    TTTTTTGATG CCCGTGAAGC ACATAGTGAT GAGAATCCAT CAGAAGGTGA TGGAGCAGTT
    AACAAGGAAG AGAAGGATGT TAATTTACGC ATCTCAGGAA ACTATTTGAT CCTTGATGGC
    TATGACCCAG TGCAGGAGAG TTCCACAGAT GAGGAGGTTG CTTCCTCACT TACCCTGCAG
    CCCATGACAG GCATCCCTGC TGTGGAATCC ACCCACCAGC AGCAACATTC TCCTCAGAAT
    ACTCACTCCG ATGGGGCAAT TTCACCATTC ACCCCCGAAT TTCTGGTCCA GCAGCGCTGG
    GGAGCTATGG AGTATTCCTG TTTTGAGATC CAGAGTCCCT CCTCTTGTGC AGATTCACAG
    AGCCAGATCA TGGAGTACAT TCATAAGATA GAGGCTGACC TTGAACACTT AAAGAAGGTG
    GAGGAAAGTT ACACCATTCT TTGCCAAAGG CTGGCTGGAT CAGCCCTCAC AGACAAGCAC
    TCAGATAAAA GTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu21676
    Clone ID Related Accession (Same CDS sequence) NM_015313.3 , NM_015313.2
    Accession Version NM_015313.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4635bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-10-01
    Organism Homo sapiens(human)
    Product rho guanine nucleotide exchange factor 12 isoform 1
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BC063117.1, AF180681.1, AK304316.1 and AP000758.5. This sequence is a reference standard in the RefSeqGene project. On Nov 2, 2010 this sequence version replaced NM_015313.1. Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF180681.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    ATGAGTGGCA CACAGTCTAC TATCACCGAC AGGTTTCCCC TCAAAAAACC TATAAGGCAT 
    GGAAGTATTT TGAACCGAGA GTCACCAACA GATAAGAAGC AGAAAGTTGA GCGCATTGCA
    TCACATGATT TTGACCCCAC AGATAGCTCC TCCAAGAAGA CAAAGTCTAG TTCAGAGGAG
    AGTAGATCCG AGATATATGG TCTTGTTCAG CGTTGCGTAA TCATCCAGAA AGATGACAAT
    GGATTTGGGC TGACGGTCAG TGGAGACAAT CCAGTCTTCG TACAGTCTGT CAAAGAAGAT
    GGAGCAGCCA TGCGGGCTGG AGTACAGACA GGTGATCGAA TCATCAAGGT GAATGGAACT
    CTGGTGACTC ATTCAAATCA TCTGGAGGTG GTGAAGCTAA TCAAATCTGG TTCCTATGTA
    GCTCTCACTG TTCAGGGACG CCCACCTGGG TCGCCCCAGA TTCCACTTGC CGACTCTGAA
    GTAGAGCCGT CAGTCATTGG ACATATGTCT CCCATCATGA CATCTCCTCA TTCACCTGGA
    GCATCTGGGA ATATGGAGAG AATCACTAGT CCTGTGCTCA TGGGGGAGGA AAACAATGTG
    GTTCATAACC AGAAAGTAGA AATTCTGAGA AAAATGTTAC AGAAAGAACA GGAACGGCTA
    CAGTTATTGC AGGAAGATTA CAACCGAACA CCTGCCCAAA GATTGCTAAA AGAGATCCAA
    GAGGCCAAGA AACACATTCC TCAGCTGCAA GAGCAGTTAT CCAAAGCCAC AGGCTCTGCT
    CAGGATGGAG CTGTAGTTAC ACCCTCCAGA CCTTTAGGGG ACACCCTAAC AGTCAGTGAG
    GCAGAAACAG ATCCTGGAGA TGTACTGGGC AGGACTGACT GTAGCAGTGG AGATGCTTCT
    CGGCCCAGTA GTGACAATGC AGATAGTCCC AAGAGTGGCC CAAAAGAGAG AATTTATCTA
    GAGGAAAACC CAGAGAAAAG TGAAACAATT CAGGACACTG ACACTCAATC ACTTGTCGGA
    AGTCCCTCAA CCCGTATAGC ACCTCATATT ATTGGAGCAG AAGATGATGA TTTTGGTACT
    GAACATGAAC AGATCAATGG ACAGTGCAGC TGTTTCCAGA GCATTGAATT ACTAAAATCT
    CGCCCGGCTC ATTTGGCTGT TTTCTTACAC CATGTAGTTT CACAATTTGA CCCTGCGACT
    TTGCTCTGTT ATCTCTATTC AGACCTGTAT AAACATACCA ATTCCAAAGA AACTCGTCGC
    ATCTTCCTTG AGTTTCATCA GTTCTTTCTA GATCGATCAG CACACCTGAA AGTTTCTGTT
    CCTGATGAAA TGTCTGCAGA TCTAGAAAAG AGAAGACCTG AGCTCATTCC TGAGGATCTG
    CATCGCCACT ATATCCAAAC TATGCAAGAA AGAGTCCATC CAGAAGTTCA AAGGCACTTA
    GAAGATTTTC GGCAGAAACG TAGTATGGGA CTGACCTTGG CTGAAAGCGA GCTGACTAAA
    CTTGATGCAG AGCGAGACAA GGACCGATTG ACTTTGGAGA AGGAGCGGAC ATGTGCAGAA
    CAGATTGTTG CCAAAATTGA AGAAGTATTG ATGACTGCTC AGGCTGTAGA GGAAGATAAG
    AGCTCCACCA TGCAGTATGT TATTCTCATG TATATGAAGC ATTTGGGAGT AAAAGTGAAA
    GAGCCTCGAA ATTTGGAGCA CAAACGGGGT CGGATTGGAT TTCTTCCCAA AATCAAGCAA
    AGTATGAAGA AAGATAAAGA AGGGGAAGAA AAAGGGAAGC GAAGAGGATT CCCCAGCATC
    CTGGGACCCC CACGGAGACC AAGCCGTCAT GACAACAGTG CAATTGGCAG AGCCATGGAA
    CTACAGAAGG CGCGCCACCC TAAGCACTTA TCCACACCCT CATCTGTGAG TCCTGAACCT
    CAGGACTCTG CCAAGTTGCG CCAGAGTGGG TTAGCAAATG AAGGAACAGA CGCTGGATAC
    CTGCCTGCCA ATTCCATGTC TTCTGTAGCT TCAGGGGCCT CTTTTTCCCA GGAAGGAGGG
    AAAGAGAATG ATACAGGATC AAAGCAAGTT GGAGAAACAT CAGCACCTGG AGACACCTTA
    GATGGCACAC CTCGTACTCT CAATACTGTC TTTGATTTCC CACCACCTCC ATTAGACCAA
    GTGCAGGAGG AGGAATGTGA AGTAGAAAGG GTGACTGAAC ATGGGACACC AAAGCCCTTT
    CGAAAGTTTG ACAGTGTAGC TTTTGGAGAA AGTCAAAGTG AGGATGAACA ATTTGAAAAT
    GACTTAGAGA CAGATCCACC CAACTGGCAG CAGCTTGTTA GTCGAGAAGT GTTACTGGGA
    CTAAAACCTT GTGAAATCAA AAGACAGGAA GTGATTAATG AATTGTTCTA CACTGAAAGA
    GCTCATGTTC GAACACTGAA GGTTCTTGAT CAAGTGTTCT ATCAGCGAGT ATCCAGAGAA
    GGAATTCTGT CACCCTCAGA GCTACGGAAA ATTTTTTCAA ACTTGGAAGA TATTCTTCAA
    CTTCATATTG GATTGAATGA ACAAATGAAG GCTGTTCGAA AGAGAAATGA GACCTCTGTT
    ATCGATCAGA TTGGGGAAGA TTTGCTGACA TGGTTCAGCG GACCAGGAGA GGAGAAATTG
    AAACATGCTG CTGCTACCTT TTGCAGTAAC CAACCTTTCG CCCTGGAAAT GATCAAATCT
    CGTCAGAAAA AGGATTCTCG ATTTCAGACT TTTGTGCAAG ATGCTGAAAG TAATCCACTG
    TGTCGTCGTC TTCAACTGAA GGATATTATT CCCACTCAAA TGCAAAGGCT TACTAAGTAC
    CCACTTCTGT TGGATAATAT TGCCAAATAC ACAGAATGGC CAACAGAAAG GGAGAAGGTG
    AAGAAAGCTG CAGATCACTG TCGTCAGATC TTAAATTATG TAAATCAGGC TGTCAAGGAG
    GCAGAAAACA AGCAGCGCCT AGAAGATTAT CAGCGTCGCC TTGATACCTC CAGCCTGAAG
    TTGTCAGAGT ACCCAAATGT TGAAGAGCTC AGGAATTTGG ATTTAACAAA AAGGAAGATG
    ATTCATGAAG GGCCATTGGT TTGGAAGGTG AATAGAGATA AAACTATTGA TTTATACACG
    TTGCTGCTGG AAGACATTCT TGTATTGTTA CAAAAGCAGG ATGATAGACT GGTTTTAAGG
    TGTCATAGTA AGATTCTGGC ATCTACAGCT GATAGCAAAC ACACGTTTAG CCCTGTCATT
    AAGTTGAGTA CAGTGTTGGT TCGACAAGTG GCAACAGATA ACAAAGCTTT ATTCGTCATT
    TCCATGTCAG ACAATGGCGC TCAGATTTAT GAACTGGTGG CACAGACAGT TTCTGAAAAG
    ACTGTCTGGC AGGACCTAAT CTGTCGGATG GCTGCATCAG TGAAGGAGCA ATCCACAAAG
    CCAATTCCAT TACCACAGTC AACACCTGGC GAAGGAGATA ATGATGAAGA AGATCCTTCA
    AAATTAAAAG AGGAGCAGCA TGGCATTTCA GTCACTGGTT TGCAGAGTCC AGACAGAGAT
    TTGGGATTAG AATCTACCTT AATATCGTCA AAACCTCAGT CTCATTCACT GAGTACCTCT
    GGGAAATCAG AGGTACGTGA TCTGTTTGTG GCTGAGAGAC AGTTTGCAAA GGAACAACAT
    ACAGATGGGA CACTAAAGGA AGTTGGAGAA GATTATCAAA TCGCAATCCC AGATTCACAC
    CTGCCTGTCT CAGAAGAACG GTGGGCATTG GATGCACTAA GAAATTTGGG TTTGTTGAAG
    CAGTTGCTGG TGCAACAGCT AGGTTTGACT GAGAAGAGCG TTCAGGAAGA CTGGCAACAT
    TTCCCAAGAT ACAGAACAGC CTCTCAGGGG CCGCAGACAG ACAGTGTCAT CCAGAACTCT
    GAAAATATTA AGGCCTATCA TTCTGGTGAA GGACATATGC CCTTTAGAAC TGGAACTGGT
    GACATTGCAA CTTGTTACAG TCCACGGACT TCAACTGAAT CTTTTGCTCC ACGGGATTCA
    GTGGGACTGG CACCCCAGGA TAGCCAGGCA AGTAACATTT TAGTAATGGA CCACATGATT
    ATGACCCCAG AGATGCCTAC CATGGAGCCA GAAGGGGGTC TTGATGACAG TGGAGAGCAC
    TTTTTTGATG CCCGTGAAGC ACATAGTGAT GAGAATCCAT CAGAAGGTGA TGGAGCAGTT
    AACAAGGAAG AGAAGGATGT TAATTTACGC ATCTCAGGAA ACTATTTGAT CCTTGATGGC
    TATGACCCAG TGCAGGAGAG TTCCACAGAT GAGGAGGTTG CTTCCTCACT TACCCTGCAG
    CCCATGACAG GCATCCCTGC TGTGGAATCC ACCCACCAGC AGCAACATTC TCCTCAGAAT
    ACTCACTCCG ATGGGGCAAT TTCACCATTC ACCCCCGAAT TTCTGGTCCA GCAGCGCTGG
    GGAGCTATGG AGTATTCCTG TTTTGAGATC CAGAGTCCCT CCTCTTGTGC AGATTCACAG
    AGCCAGATCA TGGAGTACAT TCATAAGATA GAGGCTGACC TTGAACACTT AAAGAAGGTG
    GAGGAAAGTT ACACCATTCT TTGCCAAAGG CTGGCTGGAT CAGCCCTCAC AGACAAGCAC
    TCAGATAAAA GTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_056128.1
    CDS336..4970
    Translation

    Target ORF information:

    RefSeq Version NM_015313.2
    Organism Homo sapiens(human)
    Definition Homo sapiens Rho guanine nucleotide exchange factor 12 (ARHGEF12), transcript variant 1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_015313.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    ATGAGTGGCA CACAGTCTAC TATCACCGAC AGGTTTCCCC TCAAAAAACC TATAAGGCAT 
    GGAAGTATTT TGAACCGAGA GTCACCAACA GATAAGAAGC AGAAAGTTGA GCGCATTGCA
    TCACATGATT TTGACCCCAC AGATAGCTCC TCCAAGAAGA CAAAGTCTAG TTCAGAGGAG
    AGTAGATCCG AGATATATGG TCTTGTTCAG CGTTGCGTAA TCATCCAGAA AGATGACAAT
    GGATTTGGGC TGACGGTCAG TGGAGACAAT CCAGTCTTCG TACAGTCTGT CAAAGAAGAT
    GGAGCAGCCA TGCGGGCTGG AGTACAGACA GGTGATCGAA TCATCAAGGT GAATGGAACT
    CTGGTGACTC ATTCAAATCA TCTGGAGGTG GTGAAGCTAA TCAAATCTGG TTCCTATGTA
    GCTCTCACTG TTCAGGGACG CCCACCTGGG TCGCCCCAGA TTCCACTTGC CGACTCTGAA
    GTAGAGCCGT CAGTCATTGG ACATATGTCT CCCATCATGA CATCTCCTCA TTCACCTGGA
    GCATCTGGGA ATATGGAGAG AATCACTAGT CCTGTGCTCA TGGGGGAGGA AAACAATGTG
    GTTCATAACC AGAAAGTAGA AATTCTGAGA AAAATGTTAC AGAAAGAACA GGAACGGCTA
    CAGTTATTGC AGGAAGATTA CAACCGAACA CCTGCCCAAA GATTGCTAAA AGAGATCCAA
    GAGGCCAAGA AACACATTCC TCAGCTGCAA GAGCAGTTAT CCAAAGCCAC AGGCTCTGCT
    CAGGATGGAG CTGTAGTTAC ACCCTCCAGA CCTTTAGGGG ACACCCTAAC AGTCAGTGAG
    GCAGAAACAG ATCCTGGAGA TGTACTGGGC AGGACTGACT GTAGCAGTGG AGATGCTTCT
    CGGCCCAGTA GTGACAATGC AGATAGTCCC AAGAGTGGCC CAAAAGAGAG AATTTATCTA
    GAGGAAAACC CAGAGAAAAG TGAAACAATT CAGGACACTG ACACTCAATC ACTTGTCGGA
    AGTCCCTCAA CCCGTATAGC ACCTCATATT ATTGGAGCAG AAGATGATGA TTTTGGTACT
    GAACATGAAC AGATCAATGG ACAGTGCAGC TGTTTCCAGA GCATTGAATT ACTAAAATCT
    CGCCCGGCTC ATTTGGCTGT TTTCTTACAC CATGTAGTTT CACAATTTGA CCCTGCGACT
    TTGCTCTGTT ATCTCTATTC AGACCTGTAT AAACATACCA ATTCCAAAGA AACTCGTCGC
    ATCTTCCTTG AGTTTCATCA GTTCTTTCTA GATCGATCAG CACACCTGAA AGTTTCTGTT
    CCTGATGAAA TGTCTGCAGA TCTAGAAAAG AGAAGACCTG AGCTCATTCC TGAGGATCTG
    CATCGCCACT ATATCCAAAC TATGCAAGAA AGAGTCCATC CAGAAGTTCA AAGGCACTTA
    GAAGATTTTC GGCAGAAACG TAGTATGGGA CTGACCTTGG CTGAAAGCGA GCTGACTAAA
    CTTGATGCAG AGCGAGACAA GGACCGATTG ACTTTGGAGA AGGAGCGGAC ATGTGCAGAA
    CAGATTGTTG CCAAAATTGA AGAAGTATTG ATGACTGCTC AGGCTGTAGA GGAAGATAAG
    AGCTCCACCA TGCAGTATGT TATTCTCATG TATATGAAGC ATTTGGGAGT AAAAGTGAAA
    GAGCCTCGAA ATTTGGAGCA CAAACGGGGT CGGATTGGAT TTCTTCCCAA AATCAAGCAA
    AGTATGAAGA AAGATAAAGA AGGGGAAGAA AAAGGGAAGC GAAGAGGATT CCCCAGCATC
    CTGGGACCCC CACGGAGACC AAGCCGTCAT GACAACAGTG CAATTGGCAG AGCCATGGAA
    CTACAGAAGG CGCGCCACCC TAAGCACTTA TCCACACCCT CATCTGTGAG TCCTGAACCT
    CAGGACTCTG CCAAGTTGCG CCAGAGTGGG TTAGCAAATG AAGGAACAGA CGCTGGATAC
    CTGCCTGCCA ATTCCATGTC TTCTGTAGCT TCAGGGGCCT CTTTTTCCCA GGAAGGAGGG
    AAAGAGAATG ATACAGGATC AAAGCAAGTT GGAGAAACAT CAGCACCTGG AGACACCTTA
    GATGGCACAC CTCGTACTCT CAATACTGTC TTTGATTTCC CACCACCTCC ATTAGACCAA
    GTGCAGGAGG AGGAATGTGA AGTAGAAAGG GTGACTGAAC ATGGGACACC AAAGCCCTTT
    CGAAAGTTTG ACAGTGTAGC TTTTGGAGAA AGTCAAAGTG AGGATGAACA ATTTGAAAAT
    GACTTAGAGA CAGATCCACC CAACTGGCAG CAGCTTGTTA GTCGAGAAGT GTTACTGGGA
    CTAAAACCTT GTGAAATCAA AAGACAGGAA GTGATTAATG AATTGTTCTA CACTGAAAGA
    GCTCATGTTC GAACACTGAA GGTTCTTGAT CAAGTGTTCT ATCAGCGAGT ATCCAGAGAA
    GGAATTCTGT CACCCTCAGA GCTACGGAAA ATTTTTTCAA ACTTGGAAGA TATTCTTCAA
    CTTCATATTG GATTGAATGA ACAAATGAAG GCTGTTCGAA AGAGAAATGA GACCTCTGTT
    ATCGATCAGA TTGGGGAAGA TTTGCTGACA TGGTTCAGCG GACCAGGAGA GGAGAAATTG
    AAACATGCTG CTGCTACCTT TTGCAGTAAC CAACCTTTCG CCCTGGAAAT GATCAAATCT
    CGTCAGAAAA AGGATTCTCG ATTTCAGACT TTTGTGCAAG ATGCTGAAAG TAATCCACTG
    TGTCGTCGTC TTCAACTGAA GGATATTATT CCCACTCAAA TGCAAAGGCT TACTAAGTAC
    CCACTTCTGT TGGATAATAT TGCCAAATAC ACAGAATGGC CAACAGAAAG GGAGAAGGTG
    AAGAAAGCTG CAGATCACTG TCGTCAGATC TTAAATTATG TAAATCAGGC TGTCAAGGAG
    GCAGAAAACA AGCAGCGCCT AGAAGATTAT CAGCGTCGCC TTGATACCTC CAGCCTGAAG
    TTGTCAGAGT ACCCAAATGT TGAAGAGCTC AGGAATTTGG ATTTAACAAA AAGGAAGATG
    ATTCATGAAG GGCCATTGGT TTGGAAGGTG AATAGAGATA AAACTATTGA TTTATACACG
    TTGCTGCTGG AAGACATTCT TGTATTGTTA CAAAAGCAGG ATGATAGACT GGTTTTAAGG
    TGTCATAGTA AGATTCTGGC ATCTACAGCT GATAGCAAAC ACACGTTTAG CCCTGTCATT
    AAGTTGAGTA CAGTGTTGGT TCGACAAGTG GCAACAGATA ACAAAGCTTT ATTCGTCATT
    TCCATGTCAG ACAATGGCGC TCAGATTTAT GAACTGGTGG CACAGACAGT TTCTGAAAAG
    ACTGTCTGGC AGGACCTAAT CTGTCGGATG GCTGCATCAG TGAAGGAGCA ATCCACAAAG
    CCAATTCCAT TACCACAGTC AACACCTGGC GAAGGAGATA ATGATGAAGA AGATCCTTCA
    AAATTAAAAG AGGAGCAGCA TGGCATTTCA GTCACTGGTT TGCAGAGTCC AGACAGAGAT
    TTGGGATTAG AATCTACCTT AATATCGTCA AAACCTCAGT CTCATTCACT GAGTACCTCT
    GGGAAATCAG AGGTACGTGA TCTGTTTGTG GCTGAGAGAC AGTTTGCAAA GGAACAACAT
    ACAGATGGGA CACTAAAGGA AGTTGGAGAA GATTATCAAA TCGCAATCCC AGATTCACAC
    CTGCCTGTCT CAGAAGAACG GTGGGCATTG GATGCACTAA GAAATTTGGG TTTGTTGAAG
    CAGTTGCTGG TGCAACAGCT AGGTTTGACT GAGAAGAGCG TTCAGGAAGA CTGGCAACAT
    TTCCCAAGAT ACAGAACAGC CTCTCAGGGG CCGCAGACAG ACAGTGTCAT CCAGAACTCT
    GAAAATATTA AGGCCTATCA TTCTGGTGAA GGACATATGC CCTTTAGAAC TGGAACTGGT
    GACATTGCAA CTTGTTACAG TCCACGGACT TCAACTGAAT CTTTTGCTCC ACGGGATTCA
    GTGGGACTGG CACCCCAGGA TAGCCAGGCA AGTAACATTT TAGTAATGGA CCACATGATT
    ATGACCCCAG AGATGCCTAC CATGGAGCCA GAAGGGGGTC TTGATGACAG TGGAGAGCAC
    TTTTTTGATG CCCGTGAAGC ACATAGTGAT GAGAATCCAT CAGAAGGTGA TGGAGCAGTT
    AACAAGGAAG AGAAGGATGT TAATTTACGC ATCTCAGGAA ACTATTTGAT CCTTGATGGC
    TATGACCCAG TGCAGGAGAG TTCCACAGAT GAGGAGGTTG CTTCCTCACT TACCCTGCAG
    CCCATGACAG GCATCCCTGC TGTGGAATCC ACCCACCAGC AGCAACATTC TCCTCAGAAT
    ACTCACTCCG ATGGGGCAAT TTCACCATTC ACCCCCGAAT TTCTGGTCCA GCAGCGCTGG
    GGAGCTATGG AGTATTCCTG TTTTGAGATC CAGAGTCCCT CCTCTTGTGC AGATTCACAG
    AGCCAGATCA TGGAGTACAT TCATAAGATA GAGGCTGACC TTGAACACTT AAAGAAGGTG
    GAGGAAAGTT ACACCATTCT TTGCCAAAGG CTGGCTGGAT CAGCCCTCAC AGACAAGCAC
    TCAGATAAAA GTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu92069
    Clone ID Related Accession (Same CDS sequence) XM_017017420.1
    Accession Version XM_017017420.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4458bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product rho guanine nucleotide exchange factor 12 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000011.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    ATGAGTGGCA CACAGTCTAC TATCACCGAC AGGTTTCCCC TCAAAAAACC TATAAGGCAT 
    GGAAGTATTT TGAACCGAGA GTCACCAACA GATAAGAAGC AGAAAGTTGA GCGCATTGCA
    TCACATGATT TTGACCCCAC AGGTCTTGTT CAGCGTTGCG TAATCATCCA GAAAGATGAC
    AATGGATTTG GGCTGACGGT CAGTGGAGAC AATCCAGTCT TCGTACAGTC TGTCAAAGAA
    GATGGAGCAG CCATGCGGGC TGGAGTACAG ACAGGTGATC GAATCATCAA GGTGAATGGA
    ACTCTGGTGA CTCATTCAAA TCATCTGGAG GTGGTGAAGC TAATCAAATC TGGTTCCTAT
    GTAGCTCTCA CTGTTCAGGG ACGCCCACCT GGGTCGCCCC AGATTCCACT TGCCGACTCT
    GAAGTAGAGC CGTCAGTCAT TGGACATATG TCTCCCATCA TGACATCTCC TCATTCACCT
    GGAGCATCTG GGAATATGGA GAGAATCACT AGTCCTGTGC TCATGGGGGA GGAAAACAAT
    GTGGTTCATA ACCAGAAAGT AGAAATTCTG AGAAAAATGT TACAGAAAGA ACAGGAACGG
    CTACAGGATG GAGCTGTAGT TACACCCTCC AGACCTTTAG GGGACACCCT AACAGTCAGT
    GAGGCAGAAA CAGATCCTGG AGATGTACTG GGCAGGACTG ACTGTAGCAG TGGAGATGCT
    TCTCGGCCCA GTAGTGACAA TGCAGATAGT CCCAAGAGTG GCCCAAAAGA GAGAATTTAT
    CTAGAGGAAA ACCCAGAGAA AAGTGAAACA ATTCAGGACA CTGACACTCA ATCACTTGTC
    GGAAGTCCCT CAACCCGTAT AGCACCTCAT ATTATTGGAG CAGAAGATGA TGATTTTGGT
    ACTGAACATG AACAGATCAA TGGACAGTGC AGCTGTTTCC AGAGCATTGA ATTACTAAAA
    TCTCGCCCGG CTCATTTGGC TGTTTTCTTA CACCATGTAG TTTCACAATT TGACCCTGCG
    ACTTTGCTCT GTTATCTCTA TTCAGACCTG TATAAACATA CCAATTCCAA AGAAACTCGT
    CGCATCTTCC TTGAGTTTCA TCAGTTCTTT CTAGATCGAT CAGCACACCT GAAAGTTTCT
    GTTCCTGATG AAATGTCTGC AGATCTAGAA AAGAGAAGAC CTGAGCTCAT TCCTGAGGAT
    CTGCATCGCC ACTATATCCA AACTATGCAA GAAAGAGTCC ATCCAGAAGT TCAAAGGCAC
    TTAGAAGATT TTCGGCAGAA ACGTAGTATG GGACTGACCT TGGCTGAAAG CGAGCTGACT
    AAACTTGATG CAGAGCGAGA CAAGGACCGA TTGACTTTGG AGAAGGAGCG GACATGTGCA
    GAACAGATTG TTGCCAAAAT TGAAGAAGTA TTGATGACTG CTCAGGCTGT AGAGGAAGAT
    AAGAGCTCCA CCATGCAGTA TGTTATTCTC ATGTATATGA AGCATTTGGG AGTAAAAGTG
    AAAGAGCCTC GAAATTTGGA GCACAAACGG GGTCGGATTG GATTTCTTCC CAAAATCAAG
    CAAAGTATGA AGAAAGATAA AGAAGGGGAA GAAAAAGGGA AGCGAAGAGG ATTCCCCAGC
    ATCCTGGGAC CCCCACGGAG ACCAAGCCGT CATGACAACA GTGCAATTGG CAGAGCCATG
    GAACTACAGA AGGCGCGCCA CCCTAAGCAC TTATCCACAC CCTCATCTGT GAGTCCTGAA
    CCTCAGGACT CTGCCAAGTT GCGCCAGAGT GGGTTAGCAA ATGAAGGAAC AGACGCTGGA
    TACCTGCCTG CCAATTCCAT GTCTTCTGTA GCTTCAGGGG CCTCTTTTTC CCAGGAAGGA
    GGGAAAGAGA ATGATACAGG ATCAAAGCAA GTTGGAGAAA CATCAGCACC TGGAGACACC
    TTAGATGGCA CACCTCGTAC TCTCAATACT GTCTTTGATT TCCCACCACC TCCATTAGAC
    CAAGTGCAGG AGGAGGAATG TGAAGTAGAA AGGGTGACTG AACATGGGAC ACCAAAGCCC
    TTTCGAAAGT TTGACAGTGT AGCTTTTGGA GAAAGTCAAA GTGAGGATGA ACAATTTGAA
    AATGACTTAG AGACAGATCC ACCCAACTGG CAGCAGCTTG TTAGTCGAGA AGTGTTACTG
    GGACTAAAAC CTTGTGAAAT CAAAAGACAG GAAGTGATTA ATGAATTGTT CTACACTGAA
    AGAGCTCATG TTCGAACACT GAAGGTTCTT GATCAAGTGT TCTATCAGCG AGTATCCAGA
    GAAGGAATTC TGTCACCCTC AGAGCTACGG AAAATTTTTT CAAACTTGGA AGATATTCTT
    CAACTTCATA TTGGATTGAA TGAACAAATG AAGGCTGTTC GAAAGAGAAA TGAGACCTCT
    GTTATCGATC AGATTGGGGA AGATTTGCTG ACATGGTTCA GCGGACCAGG AGAGGAGAAA
    TTGAAACATG CTGCTGCTAC CTTTTGCAGT AACCAACCTT TCGCCCTGGA AATGATCAAA
    TCTCGTCAGA AAAAGGATTC TCGATTTCAG ACTTTTGTGC AAGATGCTGA AAGTAATCCA
    CTGTGTCGTC GTCTTCAACT GAAGGATATT ATTCCCACTC AAATGCAAAG GCTTACTAAG
    TACCCACTTC TGTTGGATAA TATTGCCAAA TACACAGAAT GGCCAACAGA AAGGGAGAAG
    GTGAAGAAAG CTGCAGATCA CTGTCGTCAG ATCTTAAATT ATGTAAATCA GGCTGTCAAG
    GAGGCAGAAA ACAAGCAGCG CCTAGAAGAT TATCAGCGTC GCCTTGATAC CTCCAGCCTG
    AAGTTGTCAG AGTACCCAAA TGTTGAAGAG CTCAGGAATT TGGATTTAAC AAAAAGGAAG
    ATGATTCATG AAGGGCCATT GGTTTGGAAG GTGAATAGAG ATAAAACTAT TGATTTATAC
    ACGTTGCTGC TGGAAGACAT TCTTGTATTG TTACAAAAGC AGGATGATAG ACTGGTTTTA
    AGGTGTCATA GTAAGATTCT GGCATCTACA GCTGATAGCA AACACACGTT TAGCCCTGTC
    ATTAAGTTGA GTACAGTGTT GGTTCGACAA GTGGCAACAG ATAACAAAGC TTTATTCGTC
    ATTTCCATGT CAGACAATGG CGCTCAGATT TATGAACTGG TGGCACAGAC AGTTTCTGAA
    AAGACTGTCT GGCAGGACCT AATCTGTCGG ATGGCTGCAT CAGTGAAGGA GCAATCCACA
    AAGCCAATTC CATTACCACA GTCAACACCT GGCGAAGGAG ATAATGATGA AGAAGATCCT
    TCAAAATTAA AAGAGGAGCA GCATGGCATT TCAGTCACTG GTTTGCAGAG TCCAGACAGA
    GATTTGGGAT TAGAATCTAC CTTAATATCG TCAAAACCTC AGTCTCATTC ACTGAGTACC
    TCTGGGAAAT CAGAGGTACG TGATCTGTTT GTGGCTGAGA GACAGTTTGC AAAGGAACAA
    CATACAGATG GGACACTAAA GGAAGTTGGA GAAGATTATC AAATCGCAAT CCCAGATTCA
    CACCTGCCTG TCTCAGAAGA ACGGTGGGCA TTGGATGCAC TAAGAAATTT GGGTTTGTTG
    AAGCAGTTGC TGGTGCAACA GCTAGGTTTG ACTGAGAAGA GCGTTCAGGA AGACTGGCAA
    CATTTCCCAA GATACAGAAC AGCCTCTCAG GGGCCGCAGA CAGACAGTGT CATCCAGAAC
    TCTGAAAATA TTAAGGCCTA TCATTCTGGT GAAGGACATA TGCCCTTTAG AACTGGAACT
    GGTGACATTG CAACTTGTTA CAGTCCACGG ACTTCAACTG AATCTTTTGC TCCACGGGAT
    TCAGTGGGAC TGGCACCCCA GGATAGCCAG GCAAGTAACA TTTTAGTAAT GGACCACATG
    ATTATGACCC CAGAGATGCC TACCATGGAG CCAGAAGGGG GTCTTGATGA CAGTGGAGAG
    CACTTTTTTG ATGCCCGTGA AGCACATAGT GATGAGAATC CATCAGAAGG TGATGGAGCA
    GTTAACAAGG AAGAGAAGGA TGTTAATTTA CGCATCTCAG GAAACTATTT GATCCTTGAT
    GGCTATGACC CAGTGCAGGA GAGTTCCACA GATGAGGAGG TTGCTTCCTC ACTTACCCTG
    CAGCCCATGA CAGGCATCCC TGCTGTGGAA TCCACCCACC AGCAGCAACA TTCTCCTCAG
    AATACTCACT CCGATGGGGC AATTTCACCA TTCACCCCCG AATTTCTGGT CCAGCAGCGC
    TGGGGAGCTA TGGAGTATTC CTGTTTTGAG ATCCAGAGTC CCTCCTCTTG TGCAGATTCA
    CAGAGCCAGA TCATGGAGTA CATTCATAAG ATAGAGGCTG ACCTTGAACA CTTAAAGAAG
    GTGGAGGAAA GTTACACCAT TCTTTGCCAA AGGCTGGCTG GATCAGCCCT CACAGACAAG
    CACTCAGATA AAAGTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_016872909.1
    CDS969..5426
    Translation

    Target ORF information:

    RefSeq Version XM_017017420.1
    Organism Homo sapiens(human)
    Definition Homo sapiens Rho guanine nucleotide exchange factor 12 (ARHGEF12), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017017420.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    ATGAGTGGCA CACAGTCTAC TATCACCGAC AGGTTTCCCC TCAAAAAACC TATAAGGCAT 
    GGAAGTATTT TGAACCGAGA GTCACCAACA GATAAGAAGC AGAAAGTTGA GCGCATTGCA
    TCACATGATT TTGACCCCAC AGGTCTTGTT CAGCGTTGCG TAATCATCCA GAAAGATGAC
    AATGGATTTG GGCTGACGGT CAGTGGAGAC AATCCAGTCT TCGTACAGTC TGTCAAAGAA
    GATGGAGCAG CCATGCGGGC TGGAGTACAG ACAGGTGATC GAATCATCAA GGTGAATGGA
    ACTCTGGTGA CTCATTCAAA TCATCTGGAG GTGGTGAAGC TAATCAAATC TGGTTCCTAT
    GTAGCTCTCA CTGTTCAGGG ACGCCCACCT GGGTCGCCCC AGATTCCACT TGCCGACTCT
    GAAGTAGAGC CGTCAGTCAT TGGACATATG TCTCCCATCA TGACATCTCC TCATTCACCT
    GGAGCATCTG GGAATATGGA GAGAATCACT AGTCCTGTGC TCATGGGGGA GGAAAACAAT
    GTGGTTCATA ACCAGAAAGT AGAAATTCTG AGAAAAATGT TACAGAAAGA ACAGGAACGG
    CTACAGGATG GAGCTGTAGT TACACCCTCC AGACCTTTAG GGGACACCCT AACAGTCAGT
    GAGGCAGAAA CAGATCCTGG AGATGTACTG GGCAGGACTG ACTGTAGCAG TGGAGATGCT
    TCTCGGCCCA GTAGTGACAA TGCAGATAGT CCCAAGAGTG GCCCAAAAGA GAGAATTTAT
    CTAGAGGAAA ACCCAGAGAA AAGTGAAACA ATTCAGGACA CTGACACTCA ATCACTTGTC
    GGAAGTCCCT CAACCCGTAT AGCACCTCAT ATTATTGGAG CAGAAGATGA TGATTTTGGT
    ACTGAACATG AACAGATCAA TGGACAGTGC AGCTGTTTCC AGAGCATTGA ATTACTAAAA
    TCTCGCCCGG CTCATTTGGC TGTTTTCTTA CACCATGTAG TTTCACAATT TGACCCTGCG
    ACTTTGCTCT GTTATCTCTA TTCAGACCTG TATAAACATA CCAATTCCAA AGAAACTCGT
    CGCATCTTCC TTGAGTTTCA TCAGTTCTTT CTAGATCGAT CAGCACACCT GAAAGTTTCT
    GTTCCTGATG AAATGTCTGC AGATCTAGAA AAGAGAAGAC CTGAGCTCAT TCCTGAGGAT
    CTGCATCGCC ACTATATCCA AACTATGCAA GAAAGAGTCC ATCCAGAAGT TCAAAGGCAC
    TTAGAAGATT TTCGGCAGAA ACGTAGTATG GGACTGACCT TGGCTGAAAG CGAGCTGACT
    AAACTTGATG CAGAGCGAGA CAAGGACCGA TTGACTTTGG AGAAGGAGCG GACATGTGCA
    GAACAGATTG TTGCCAAAAT TGAAGAAGTA TTGATGACTG CTCAGGCTGT AGAGGAAGAT
    AAGAGCTCCA CCATGCAGTA TGTTATTCTC ATGTATATGA AGCATTTGGG AGTAAAAGTG
    AAAGAGCCTC GAAATTTGGA GCACAAACGG GGTCGGATTG GATTTCTTCC CAAAATCAAG
    CAAAGTATGA AGAAAGATAA AGAAGGGGAA GAAAAAGGGA AGCGAAGAGG ATTCCCCAGC
    ATCCTGGGAC CCCCACGGAG ACCAAGCCGT CATGACAACA GTGCAATTGG CAGAGCCATG
    GAACTACAGA AGGCGCGCCA CCCTAAGCAC TTATCCACAC CCTCATCTGT GAGTCCTGAA
    CCTCAGGACT CTGCCAAGTT GCGCCAGAGT GGGTTAGCAA ATGAAGGAAC AGACGCTGGA
    TACCTGCCTG CCAATTCCAT GTCTTCTGTA GCTTCAGGGG CCTCTTTTTC CCAGGAAGGA
    GGGAAAGAGA ATGATACAGG ATCAAAGCAA GTTGGAGAAA CATCAGCACC TGGAGACACC
    TTAGATGGCA CACCTCGTAC TCTCAATACT GTCTTTGATT TCCCACCACC TCCATTAGAC
    CAAGTGCAGG AGGAGGAATG TGAAGTAGAA AGGGTGACTG AACATGGGAC ACCAAAGCCC
    TTTCGAAAGT TTGACAGTGT AGCTTTTGGA GAAAGTCAAA GTGAGGATGA ACAATTTGAA
    AATGACTTAG AGACAGATCC ACCCAACTGG CAGCAGCTTG TTAGTCGAGA AGTGTTACTG
    GGACTAAAAC CTTGTGAAAT CAAAAGACAG GAAGTGATTA ATGAATTGTT CTACACTGAA
    AGAGCTCATG TTCGAACACT GAAGGTTCTT GATCAAGTGT TCTATCAGCG AGTATCCAGA
    GAAGGAATTC TGTCACCCTC AGAGCTACGG AAAATTTTTT CAAACTTGGA AGATATTCTT
    CAACTTCATA TTGGATTGAA TGAACAAATG AAGGCTGTTC GAAAGAGAAA TGAGACCTCT
    GTTATCGATC AGATTGGGGA AGATTTGCTG ACATGGTTCA GCGGACCAGG AGAGGAGAAA
    TTGAAACATG CTGCTGCTAC CTTTTGCAGT AACCAACCTT TCGCCCTGGA AATGATCAAA
    TCTCGTCAGA AAAAGGATTC TCGATTTCAG ACTTTTGTGC AAGATGCTGA AAGTAATCCA
    CTGTGTCGTC GTCTTCAACT GAAGGATATT ATTCCCACTC AAATGCAAAG GCTTACTAAG
    TACCCACTTC TGTTGGATAA TATTGCCAAA TACACAGAAT GGCCAACAGA AAGGGAGAAG
    GTGAAGAAAG CTGCAGATCA CTGTCGTCAG ATCTTAAATT ATGTAAATCA GGCTGTCAAG
    GAGGCAGAAA ACAAGCAGCG CCTAGAAGAT TATCAGCGTC GCCTTGATAC CTCCAGCCTG
    AAGTTGTCAG AGTACCCAAA TGTTGAAGAG CTCAGGAATT TGGATTTAAC AAAAAGGAAG
    ATGATTCATG AAGGGCCATT GGTTTGGAAG GTGAATAGAG ATAAAACTAT TGATTTATAC
    ACGTTGCTGC TGGAAGACAT TCTTGTATTG TTACAAAAGC AGGATGATAG ACTGGTTTTA
    AGGTGTCATA GTAAGATTCT GGCATCTACA GCTGATAGCA AACACACGTT TAGCCCTGTC
    ATTAAGTTGA GTACAGTGTT GGTTCGACAA GTGGCAACAG ATAACAAAGC TTTATTCGTC
    ATTTCCATGT CAGACAATGG CGCTCAGATT TATGAACTGG TGGCACAGAC AGTTTCTGAA
    AAGACTGTCT GGCAGGACCT AATCTGTCGG ATGGCTGCAT CAGTGAAGGA GCAATCCACA
    AAGCCAATTC CATTACCACA GTCAACACCT GGCGAAGGAG ATAATGATGA AGAAGATCCT
    TCAAAATTAA AAGAGGAGCA GCATGGCATT TCAGTCACTG GTTTGCAGAG TCCAGACAGA
    GATTTGGGAT TAGAATCTAC CTTAATATCG TCAAAACCTC AGTCTCATTC ACTGAGTACC
    TCTGGGAAAT CAGAGGTACG TGATCTGTTT GTGGCTGAGA GACAGTTTGC AAAGGAACAA
    CATACAGATG GGACACTAAA GGAAGTTGGA GAAGATTATC AAATCGCAAT CCCAGATTCA
    CACCTGCCTG TCTCAGAAGA ACGGTGGGCA TTGGATGCAC TAAGAAATTT GGGTTTGTTG
    AAGCAGTTGC TGGTGCAACA GCTAGGTTTG ACTGAGAAGA GCGTTCAGGA AGACTGGCAA
    CATTTCCCAA GATACAGAAC AGCCTCTCAG GGGCCGCAGA CAGACAGTGT CATCCAGAAC
    TCTGAAAATA TTAAGGCCTA TCATTCTGGT GAAGGACATA TGCCCTTTAG AACTGGAACT
    GGTGACATTG CAACTTGTTA CAGTCCACGG ACTTCAACTG AATCTTTTGC TCCACGGGAT
    TCAGTGGGAC TGGCACCCCA GGATAGCCAG GCAAGTAACA TTTTAGTAAT GGACCACATG
    ATTATGACCC CAGAGATGCC TACCATGGAG CCAGAAGGGG GTCTTGATGA CAGTGGAGAG
    CACTTTTTTG ATGCCCGTGA AGCACATAGT GATGAGAATC CATCAGAAGG TGATGGAGCA
    GTTAACAAGG AAGAGAAGGA TGTTAATTTA CGCATCTCAG GAAACTATTT GATCCTTGAT
    GGCTATGACC CAGTGCAGGA GAGTTCCACA GATGAGGAGG TTGCTTCCTC ACTTACCCTG
    CAGCCCATGA CAGGCATCCC TGCTGTGGAA TCCACCCACC AGCAGCAACA TTCTCCTCAG
    AATACTCACT CCGATGGGGC AATTTCACCA TTCACCCCCG AATTTCTGGT CCAGCAGCGC
    TGGGGAGCTA TGGAGTATTC CTGTTTTGAG ATCCAGAGTC CCTCCTCTTG TGCAGATTCA
    CAGAGCCAGA TCATGGAGTA CATTCATAAG ATAGAGGCTG ACCTTGAACA CTTAAAGAAG
    GTGGAGGAAA GTTACACCAT TCTTTGCCAA AGGCTGGCTG GATCAGCCCT CACAGACAAG
    CACTCAGATA AAAGTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu92070
    Clone ID Related Accession (Same CDS sequence) XM_017017422.1 , XM_017017422.2
    Accession Version XM_017017422.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4206bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-05
    Organism Homo sapiens(human)
    Product rho guanine nucleotide exchange factor 12 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000011.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    ATGCGGGCTG GAGTACAGAC AGGTGATCGA ATCATCAAGG TGAATGGAAC TCTGGTGACT 
    CATTCAAATC ATCTGGAGGT GGTGAAGCTA ATCAAATCTG GTTCCTATGT AGCTCTCACT
    GTTCAGGGAC GCCCACCTGG GTCGCCCCAG ATTCCACTTG CCGACTCTGA AGTAGAGCCG
    TCAGTCATTG GACATATGTC TCCCATCATG ACATCTCCTC ATTCACCTGG AGCATCTGGG
    AATATGGAGA GAATCACTAG TCCTGTGCTC ATGGGGGAGG AAAACAATGT GGTTCATAAC
    CAGAAAGTAG AAATTCTGAG AAAAATGTTA CAGAAAGAAC AGGAACGGCT ACAGGATGGA
    GCTGTAGTTA CACCCTCCAG ACCTTTAGGG GACACCCTAA CAGTCAGTGA GGCAGAAACA
    GATCCTGGAG ATGTACTGGG CAGGACTGAC TGTAGCAGTG GAGATGCTTC TCGGCCCAGT
    AGTGACAATG CAGATAGTCC CAAGAGTGGC CCAAAAGAGA GAATTTATCT AGAGGAAAAC
    CCAGAGAAAA GTGAAACAAT TCAGGACACT GACACTCAAT CACTTGTCGG AAGTCCCTCA
    ACCCGTATAG CACCTCATAT TATTGGAGCA GAAGATGATG ATTTTGGTAC TGAACATGAA
    CAGATCAATG GACAGTGCAG CTGTTTCCAG AGCATTGAAT TACTAAAATC TCGCCCGGCT
    CATTTGGCTG TTTTCTTACA CCATGTAGTT TCACAATTTG ACCCTGCGAC TTTGCTCTGT
    TATCTCTATT CAGACCTGTA TAAACATACC AATTCCAAAG AAACTCGTCG CATCTTCCTT
    GAGTTTCATC AGTTCTTTCT AGATCGATCA GCACACCTGA AAGTTTCTGT TCCTGATGAA
    ATGTCTGCAG ATCTAGAAAA GAGAAGACCT GAGCTCATTC CTGAGGATCT GCATCGCCAC
    TATATCCAAA CTATGCAAGA AAGAGTCCAT CCAGAAGTTC AAAGGCACTT AGAAGATTTT
    CGGCAGAAAC GTAGTATGGG ACTGACCTTG GCTGAAAGCG AGCTGACTAA ACTTGATGCA
    GAGCGAGACA AGGACCGATT GACTTTGGAG AAGGAGCGGA CATGTGCAGA ACAGATTGTT
    GCCAAAATTG AAGAAGTATT GATGACTGCT CAGGCTGTAG AGGAAGATAA GAGCTCCACC
    ATGCAGTATG TTATTCTCAT GTATATGAAG CATTTGGGAG TAAAAGTGAA AGAGCCTCGA
    AATTTGGAGC ACAAACGGGG TCGGATTGGA TTTCTTCCCA AAATCAAGCA AAGTATGAAG
    AAAGATAAAG AAGGGGAAGA AAAAGGGAAG CGAAGAGGAT TCCCCAGCAT CCTGGGACCC
    CCACGGAGAC CAAGCCGTCA TGACAACAGT GCAATTGGCA GAGCCATGGA ACTACAGAAG
    GCGCGCCACC CTAAGCACTT ATCCACACCC TCATCTGTGA GTCCTGAACC TCAGGACTCT
    GCCAAGTTGC GCCAGAGTGG GTTAGCAAAT GAAGGAACAG ACGCTGGATA CCTGCCTGCC
    AATTCCATGT CTTCTGTAGC TTCAGGGGCC TCTTTTTCCC AGGAAGGAGG GAAAGAGAAT
    GATACAGGAT CAAAGCAAGT TGGAGAAACA TCAGCACCTG GAGACACCTT AGATGGCACA
    CCTCGTACTC TCAATACTGT CTTTGATTTC CCACCACCTC CATTAGACCA AGTGCAGGAG
    GAGGAATGTG AAGTAGAAAG GGTGACTGAA CATGGGACAC CAAAGCCCTT TCGAAAGTTT
    GACAGTGTAG CTTTTGGAGA AAGTCAAAGT GAGGATGAAC AATTTGAAAA TGACTTAGAG
    ACAGATCCAC CCAACTGGCA GCAGCTTGTT AGTCGAGAAG TGTTACTGGG ACTAAAACCT
    TGTGAAATCA AAAGACAGGA AGTGATTAAT GAATTGTTCT ACACTGAAAG AGCTCATGTT
    CGAACACTGA AGGTTCTTGA TCAAGTGTTC TATCAGCGAG TATCCAGAGA AGGAATTCTG
    TCACCCTCAG AGCTACGGAA AATTTTTTCA AACTTGGAAG ATATTCTTCA ACTTCATATT
    GGATTGAATG AACAAATGAA GGCTGTTCGA AAGAGAAATG AGACCTCTGT TATCGATCAG
    ATTGGGGAAG ATTTGCTGAC ATGGTTCAGC GGACCAGGAG AGGAGAAATT GAAACATGCT
    GCTGCTACCT TTTGCAGTAA CCAACCTTTC GCCCTGGAAA TGATCAAATC TCGTCAGAAA
    AAGGATTCTC GATTTCAGAC TTTTGTGCAA GATGCTGAAA GTAATCCACT GTGTCGTCGT
    CTTCAACTGA AGGATATTAT TCCCACTCAA ATGCAAAGGC TTACTAAGTA CCCACTTCTG
    TTGGATAATA TTGCCAAATA CACAGAATGG CCAACAGAAA GGGAGAAGGT GAAGAAAGCT
    GCAGATCACT GTCGTCAGAT CTTAAATTAT GTAAATCAGG CTGTCAAGGA GGCAGAAAAC
    AAGCAGCGCC TAGAAGATTA TCAGCGTCGC CTTGATACCT CCAGCCTGAA GTTGTCAGAG
    TACCCAAATG TTGAAGAGCT CAGGAATTTG GATTTAACAA AAAGGAAGAT GATTCATGAA
    GGGCCATTGG TTTGGAAGGT GAATAGAGAT AAAACTATTG ATTTATACAC GTTGCTGCTG
    GAAGACATTC TTGTATTGTT ACAAAAGCAG GATGATAGAC TGGTTTTAAG GTGTCATAGT
    AAGATTCTGG CATCTACAGC TGATAGCAAA CACACGTTTA GCCCTGTCAT TAAGTTGAGT
    ACAGTGTTGG TTCGACAAGT GGCAACAGAT AACAAAGCTT TATTCGTCAT TTCCATGTCA
    GACAATGGCG CTCAGATTTA TGAACTGGTG GCACAGACAG TTTCTGAAAA GACTGTCTGG
    CAGGACCTAA TCTGTCGGAT GGCTGCATCA GTGAAGGAGC AATCCACAAA GCCAATTCCA
    TTACCACAGT CAACACCTGG CGAAGGAGAT AATGATGAAG AAGATCCTTC AAAATTAAAA
    GAGGAGCAGC ATGGCATTTC AGTCACTGGT TTGCAGAGTC CAGACAGAGA TTTGGGATTA
    GAATCTACCT TAATATCGTC AAAACCTCAG TCTCATTCAC TGAGTACCTC TGGGAAATCA
    GAGGTACGTG ATCTGTTTGT GGCTGAGAGA CAGTTTGCAA AGGAACAACA TACAGATGGG
    ACACTAAAGG AAGTTGGAGA AGATTATCAA ATCGCAATCC CAGATTCACA CCTGCCTGTC
    TCAGAAGAAC GGTGGGCATT GGATGCACTA AGAAATTTGG GTTTGTTGAA GCAGTTGCTG
    GTGCAACAGC TAGGTTTGAC TGAGAAGAGC GTTCAGGAAG ACTGGCAACA TTTCCCAAGA
    TACAGAACAG CCTCTCAGGG GCCGCAGACA GACAGTGTCA TCCAGAACTC TGAAAATATT
    AAGGCCTATC ATTCTGGTGA AGGACATATG CCCTTTAGAA CTGGAACTGG TGACATTGCA
    ACTTGTTACA GTCCACGGAC TTCAACTGAA TCTTTTGCTC CACGGGATTC AGTGGGACTG
    GCACCCCAGG ATAGCCAGGC AAGTAACATT TTAGTAATGG ACCACATGAT TATGACCCCA
    GAGATGCCTA CCATGGAGCC AGAAGGGGGT CTTGATGACA GTGGAGAGCA CTTTTTTGAT
    GCCCGTGAAG CACATAGTGA TGAGAATCCA TCAGAAGGTG ATGGAGCAGT TAACAAGGAA
    GAGAAGGATG TTAATTTACG CATCTCAGGA AACTATTTGA TCCTTGATGG CTATGACCCA
    GTGCAGGAGA GTTCCACAGA TGAGGAGGTT GCTTCCTCAC TTACCCTGCA GCCCATGACA
    GGCATCCCTG CTGTGGAATC CACCCACCAG CAGCAACATT CTCCTCAGAA TACTCACTCC
    GATGGGGCAA TTTCACCATT CACCCCCGAA TTTCTGGTCC AGCAGCGCTG GGGAGCTATG
    GAGTATTCCT GTTTTGAGAT CCAGAGTCCC TCCTCTTGTG CAGATTCACA GAGCCAGATC
    ATGGAGTACA TTCATAAGAT AGAGGCTGAC CTTGAACACT TAAAGAAGGT GGAGGAAAGT
    TACACCATTC TTTGCCAAAG GCTGGCTGGA TCAGCCCTCA CAGACAAGCA CTCAGATAAA
    AGTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_016872911.1
    CDS473..4678
    Translation

    Target ORF information:

    RefSeq Version XM_017017422.1
    Organism Homo sapiens(human)
    Definition Homo sapiens Rho guanine nucleotide exchange factor 12 (ARHGEF12), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017017422.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    ATGCGGGCTG GAGTACAGAC AGGTGATCGA ATCATCAAGG TGAATGGAAC TCTGGTGACT 
    CATTCAAATC ATCTGGAGGT GGTGAAGCTA ATCAAATCTG GTTCCTATGT AGCTCTCACT
    GTTCAGGGAC GCCCACCTGG GTCGCCCCAG ATTCCACTTG CCGACTCTGA AGTAGAGCCG
    TCAGTCATTG GACATATGTC TCCCATCATG ACATCTCCTC ATTCACCTGG AGCATCTGGG
    AATATGGAGA GAATCACTAG TCCTGTGCTC ATGGGGGAGG AAAACAATGT GGTTCATAAC
    CAGAAAGTAG AAATTCTGAG AAAAATGTTA CAGAAAGAAC AGGAACGGCT ACAGGATGGA
    GCTGTAGTTA CACCCTCCAG ACCTTTAGGG GACACCCTAA CAGTCAGTGA GGCAGAAACA
    GATCCTGGAG ATGTACTGGG CAGGACTGAC TGTAGCAGTG GAGATGCTTC TCGGCCCAGT
    AGTGACAATG CAGATAGTCC CAAGAGTGGC CCAAAAGAGA GAATTTATCT AGAGGAAAAC
    CCAGAGAAAA GTGAAACAAT TCAGGACACT GACACTCAAT CACTTGTCGG AAGTCCCTCA
    ACCCGTATAG CACCTCATAT TATTGGAGCA GAAGATGATG ATTTTGGTAC TGAACATGAA
    CAGATCAATG GACAGTGCAG CTGTTTCCAG AGCATTGAAT TACTAAAATC TCGCCCGGCT
    CATTTGGCTG TTTTCTTACA CCATGTAGTT TCACAATTTG ACCCTGCGAC TTTGCTCTGT
    TATCTCTATT CAGACCTGTA TAAACATACC AATTCCAAAG AAACTCGTCG CATCTTCCTT
    GAGTTTCATC AGTTCTTTCT AGATCGATCA GCACACCTGA AAGTTTCTGT TCCTGATGAA
    ATGTCTGCAG ATCTAGAAAA GAGAAGACCT GAGCTCATTC CTGAGGATCT GCATCGCCAC
    TATATCCAAA CTATGCAAGA AAGAGTCCAT CCAGAAGTTC AAAGGCACTT AGAAGATTTT
    CGGCAGAAAC GTAGTATGGG ACTGACCTTG GCTGAAAGCG AGCTGACTAA ACTTGATGCA
    GAGCGAGACA AGGACCGATT GACTTTGGAG AAGGAGCGGA CATGTGCAGA ACAGATTGTT
    GCCAAAATTG AAGAAGTATT GATGACTGCT CAGGCTGTAG AGGAAGATAA GAGCTCCACC
    ATGCAGTATG TTATTCTCAT GTATATGAAG CATTTGGGAG TAAAAGTGAA AGAGCCTCGA
    AATTTGGAGC ACAAACGGGG TCGGATTGGA TTTCTTCCCA AAATCAAGCA AAGTATGAAG
    AAAGATAAAG AAGGGGAAGA AAAAGGGAAG CGAAGAGGAT TCCCCAGCAT CCTGGGACCC
    CCACGGAGAC CAAGCCGTCA TGACAACAGT GCAATTGGCA GAGCCATGGA ACTACAGAAG
    GCGCGCCACC CTAAGCACTT ATCCACACCC TCATCTGTGA GTCCTGAACC TCAGGACTCT
    GCCAAGTTGC GCCAGAGTGG GTTAGCAAAT GAAGGAACAG ACGCTGGATA CCTGCCTGCC
    AATTCCATGT CTTCTGTAGC TTCAGGGGCC TCTTTTTCCC AGGAAGGAGG GAAAGAGAAT
    GATACAGGAT CAAAGCAAGT TGGAGAAACA TCAGCACCTG GAGACACCTT AGATGGCACA
    CCTCGTACTC TCAATACTGT CTTTGATTTC CCACCACCTC CATTAGACCA AGTGCAGGAG
    GAGGAATGTG AAGTAGAAAG GGTGACTGAA CATGGGACAC CAAAGCCCTT TCGAAAGTTT
    GACAGTGTAG CTTTTGGAGA AAGTCAAAGT GAGGATGAAC AATTTGAAAA TGACTTAGAG
    ACAGATCCAC CCAACTGGCA GCAGCTTGTT AGTCGAGAAG TGTTACTGGG ACTAAAACCT
    TGTGAAATCA AAAGACAGGA AGTGATTAAT GAATTGTTCT ACACTGAAAG AGCTCATGTT
    CGAACACTGA AGGTTCTTGA TCAAGTGTTC TATCAGCGAG TATCCAGAGA AGGAATTCTG
    TCACCCTCAG AGCTACGGAA AATTTTTTCA AACTTGGAAG ATATTCTTCA ACTTCATATT
    GGATTGAATG AACAAATGAA GGCTGTTCGA AAGAGAAATG AGACCTCTGT TATCGATCAG
    ATTGGGGAAG ATTTGCTGAC ATGGTTCAGC GGACCAGGAG AGGAGAAATT GAAACATGCT
    GCTGCTACCT TTTGCAGTAA CCAACCTTTC GCCCTGGAAA TGATCAAATC TCGTCAGAAA
    AAGGATTCTC GATTTCAGAC TTTTGTGCAA GATGCTGAAA GTAATCCACT GTGTCGTCGT
    CTTCAACTGA AGGATATTAT TCCCACTCAA ATGCAAAGGC TTACTAAGTA CCCACTTCTG
    TTGGATAATA TTGCCAAATA CACAGAATGG CCAACAGAAA GGGAGAAGGT GAAGAAAGCT
    GCAGATCACT GTCGTCAGAT CTTAAATTAT GTAAATCAGG CTGTCAAGGA GGCAGAAAAC
    AAGCAGCGCC TAGAAGATTA TCAGCGTCGC CTTGATACCT CCAGCCTGAA GTTGTCAGAG
    TACCCAAATG TTGAAGAGCT CAGGAATTTG GATTTAACAA AAAGGAAGAT GATTCATGAA
    GGGCCATTGG TTTGGAAGGT GAATAGAGAT AAAACTATTG ATTTATACAC GTTGCTGCTG
    GAAGACATTC TTGTATTGTT ACAAAAGCAG GATGATAGAC TGGTTTTAAG GTGTCATAGT
    AAGATTCTGG CATCTACAGC TGATAGCAAA CACACGTTTA GCCCTGTCAT TAAGTTGAGT
    ACAGTGTTGG TTCGACAAGT GGCAACAGAT AACAAAGCTT TATTCGTCAT TTCCATGTCA
    GACAATGGCG CTCAGATTTA TGAACTGGTG GCACAGACAG TTTCTGAAAA GACTGTCTGG
    CAGGACCTAA TCTGTCGGAT GGCTGCATCA GTGAAGGAGC AATCCACAAA GCCAATTCCA
    TTACCACAGT CAACACCTGG CGAAGGAGAT AATGATGAAG AAGATCCTTC AAAATTAAAA
    GAGGAGCAGC ATGGCATTTC AGTCACTGGT TTGCAGAGTC CAGACAGAGA TTTGGGATTA
    GAATCTACCT TAATATCGTC AAAACCTCAG TCTCATTCAC TGAGTACCTC TGGGAAATCA
    GAGGTACGTG ATCTGTTTGT GGCTGAGAGA CAGTTTGCAA AGGAACAACA TACAGATGGG
    ACACTAAAGG AAGTTGGAGA AGATTATCAA ATCGCAATCC CAGATTCACA CCTGCCTGTC
    TCAGAAGAAC GGTGGGCATT GGATGCACTA AGAAATTTGG GTTTGTTGAA GCAGTTGCTG
    GTGCAACAGC TAGGTTTGAC TGAGAAGAGC GTTCAGGAAG ACTGGCAACA TTTCCCAAGA
    TACAGAACAG CCTCTCAGGG GCCGCAGACA GACAGTGTCA TCCAGAACTC TGAAAATATT
    AAGGCCTATC ATTCTGGTGA AGGACATATG CCCTTTAGAA CTGGAACTGG TGACATTGCA
    ACTTGTTACA GTCCACGGAC TTCAACTGAA TCTTTTGCTC CACGGGATTC AGTGGGACTG
    GCACCCCAGG ATAGCCAGGC AAGTAACATT TTAGTAATGG ACCACATGAT TATGACCCCA
    GAGATGCCTA CCATGGAGCC AGAAGGGGGT CTTGATGACA GTGGAGAGCA CTTTTTTGAT
    GCCCGTGAAG CACATAGTGA TGAGAATCCA TCAGAAGGTG ATGGAGCAGT TAACAAGGAA
    GAGAAGGATG TTAATTTACG CATCTCAGGA AACTATTTGA TCCTTGATGG CTATGACCCA
    GTGCAGGAGA GTTCCACAGA TGAGGAGGTT GCTTCCTCAC TTACCCTGCA GCCCATGACA
    GGCATCCCTG CTGTGGAATC CACCCACCAG CAGCAACATT CTCCTCAGAA TACTCACTCC
    GATGGGGCAA TTTCACCATT CACCCCCGAA TTTCTGGTCC AGCAGCGCTG GGGAGCTATG
    GAGTATTCCT GTTTTGAGAT CCAGAGTCCC TCCTCTTGTG CAGATTCACA GAGCCAGATC
    ATGGAGTACA TTCATAAGAT AGAGGCTGAC CTTGAACACT TAAAGAAGGT GGAGGAAAGT
    TACACCATTC TTTGCCAAAG GCTGGCTGGA TCAGCCCTCA CAGACAAGCA CTCAGATAAA
    AGTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu92070
    Clone ID Related Accession (Same CDS sequence) XM_017017422.1 , XM_017017422.2
    Accession Version XM_017017422.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4206bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product rho guanine nucleotide exchange factor 12 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000011.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_017017422.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    ATGCGGGCTG GAGTACAGAC AGGTGATCGA ATCATCAAGG TGAATGGAAC TCTGGTGACT 
    CATTCAAATC ATCTGGAGGT GGTGAAGCTA ATCAAATCTG GTTCCTATGT AGCTCTCACT
    GTTCAGGGAC GCCCACCTGG GTCGCCCCAG ATTCCACTTG CCGACTCTGA AGTAGAGCCG
    TCAGTCATTG GACATATGTC TCCCATCATG ACATCTCCTC ATTCACCTGG AGCATCTGGG
    AATATGGAGA GAATCACTAG TCCTGTGCTC ATGGGGGAGG AAAACAATGT GGTTCATAAC
    CAGAAAGTAG AAATTCTGAG AAAAATGTTA CAGAAAGAAC AGGAACGGCT ACAGGATGGA
    GCTGTAGTTA CACCCTCCAG ACCTTTAGGG GACACCCTAA CAGTCAGTGA GGCAGAAACA
    GATCCTGGAG ATGTACTGGG CAGGACTGAC TGTAGCAGTG GAGATGCTTC TCGGCCCAGT
    AGTGACAATG CAGATAGTCC CAAGAGTGGC CCAAAAGAGA GAATTTATCT AGAGGAAAAC
    CCAGAGAAAA GTGAAACAAT TCAGGACACT GACACTCAAT CACTTGTCGG AAGTCCCTCA
    ACCCGTATAG CACCTCATAT TATTGGAGCA GAAGATGATG ATTTTGGTAC TGAACATGAA
    CAGATCAATG GACAGTGCAG CTGTTTCCAG AGCATTGAAT TACTAAAATC TCGCCCGGCT
    CATTTGGCTG TTTTCTTACA CCATGTAGTT TCACAATTTG ACCCTGCGAC TTTGCTCTGT
    TATCTCTATT CAGACCTGTA TAAACATACC AATTCCAAAG AAACTCGTCG CATCTTCCTT
    GAGTTTCATC AGTTCTTTCT AGATCGATCA GCACACCTGA AAGTTTCTGT TCCTGATGAA
    ATGTCTGCAG ATCTAGAAAA GAGAAGACCT GAGCTCATTC CTGAGGATCT GCATCGCCAC
    TATATCCAAA CTATGCAAGA AAGAGTCCAT CCAGAAGTTC AAAGGCACTT AGAAGATTTT
    CGGCAGAAAC GTAGTATGGG ACTGACCTTG GCTGAAAGCG AGCTGACTAA ACTTGATGCA
    GAGCGAGACA AGGACCGATT GACTTTGGAG AAGGAGCGGA CATGTGCAGA ACAGATTGTT
    GCCAAAATTG AAGAAGTATT GATGACTGCT CAGGCTGTAG AGGAAGATAA GAGCTCCACC
    ATGCAGTATG TTATTCTCAT GTATATGAAG CATTTGGGAG TAAAAGTGAA AGAGCCTCGA
    AATTTGGAGC ACAAACGGGG TCGGATTGGA TTTCTTCCCA AAATCAAGCA AAGTATGAAG
    AAAGATAAAG AAGGGGAAGA AAAAGGGAAG CGAAGAGGAT TCCCCAGCAT CCTGGGACCC
    CCACGGAGAC CAAGCCGTCA TGACAACAGT GCAATTGGCA GAGCCATGGA ACTACAGAAG
    GCGCGCCACC CTAAGCACTT ATCCACACCC TCATCTGTGA GTCCTGAACC TCAGGACTCT
    GCCAAGTTGC GCCAGAGTGG GTTAGCAAAT GAAGGAACAG ACGCTGGATA CCTGCCTGCC
    AATTCCATGT CTTCTGTAGC TTCAGGGGCC TCTTTTTCCC AGGAAGGAGG GAAAGAGAAT
    GATACAGGAT CAAAGCAAGT TGGAGAAACA TCAGCACCTG GAGACACCTT AGATGGCACA
    CCTCGTACTC TCAATACTGT CTTTGATTTC CCACCACCTC CATTAGACCA AGTGCAGGAG
    GAGGAATGTG AAGTAGAAAG GGTGACTGAA CATGGGACAC CAAAGCCCTT TCGAAAGTTT
    GACAGTGTAG CTTTTGGAGA AAGTCAAAGT GAGGATGAAC AATTTGAAAA TGACTTAGAG
    ACAGATCCAC CCAACTGGCA GCAGCTTGTT AGTCGAGAAG TGTTACTGGG ACTAAAACCT
    TGTGAAATCA AAAGACAGGA AGTGATTAAT GAATTGTTCT ACACTGAAAG AGCTCATGTT
    CGAACACTGA AGGTTCTTGA TCAAGTGTTC TATCAGCGAG TATCCAGAGA AGGAATTCTG
    TCACCCTCAG AGCTACGGAA AATTTTTTCA AACTTGGAAG ATATTCTTCA ACTTCATATT
    GGATTGAATG AACAAATGAA GGCTGTTCGA AAGAGAAATG AGACCTCTGT TATCGATCAG
    ATTGGGGAAG ATTTGCTGAC ATGGTTCAGC GGACCAGGAG AGGAGAAATT GAAACATGCT
    GCTGCTACCT TTTGCAGTAA CCAACCTTTC GCCCTGGAAA TGATCAAATC TCGTCAGAAA
    AAGGATTCTC GATTTCAGAC TTTTGTGCAA GATGCTGAAA GTAATCCACT GTGTCGTCGT
    CTTCAACTGA AGGATATTAT TCCCACTCAA ATGCAAAGGC TTACTAAGTA CCCACTTCTG
    TTGGATAATA TTGCCAAATA CACAGAATGG CCAACAGAAA GGGAGAAGGT GAAGAAAGCT
    GCAGATCACT GTCGTCAGAT CTTAAATTAT GTAAATCAGG CTGTCAAGGA GGCAGAAAAC
    AAGCAGCGCC TAGAAGATTA TCAGCGTCGC CTTGATACCT CCAGCCTGAA GTTGTCAGAG
    TACCCAAATG TTGAAGAGCT CAGGAATTTG GATTTAACAA AAAGGAAGAT GATTCATGAA
    GGGCCATTGG TTTGGAAGGT GAATAGAGAT AAAACTATTG ATTTATACAC GTTGCTGCTG
    GAAGACATTC TTGTATTGTT ACAAAAGCAG GATGATAGAC TGGTTTTAAG GTGTCATAGT
    AAGATTCTGG CATCTACAGC TGATAGCAAA CACACGTTTA GCCCTGTCAT TAAGTTGAGT
    ACAGTGTTGG TTCGACAAGT GGCAACAGAT AACAAAGCTT TATTCGTCAT TTCCATGTCA
    GACAATGGCG CTCAGATTTA TGAACTGGTG GCACAGACAG TTTCTGAAAA GACTGTCTGG
    CAGGACCTAA TCTGTCGGAT GGCTGCATCA GTGAAGGAGC AATCCACAAA GCCAATTCCA
    TTACCACAGT CAACACCTGG CGAAGGAGAT AATGATGAAG AAGATCCTTC AAAATTAAAA
    GAGGAGCAGC ATGGCATTTC AGTCACTGGT TTGCAGAGTC CAGACAGAGA TTTGGGATTA
    GAATCTACCT TAATATCGTC AAAACCTCAG TCTCATTCAC TGAGTACCTC TGGGAAATCA
    GAGGTACGTG ATCTGTTTGT GGCTGAGAGA CAGTTTGCAA AGGAACAACA TACAGATGGG
    ACACTAAAGG AAGTTGGAGA AGATTATCAA ATCGCAATCC CAGATTCACA CCTGCCTGTC
    TCAGAAGAAC GGTGGGCATT GGATGCACTA AGAAATTTGG GTTTGTTGAA GCAGTTGCTG
    GTGCAACAGC TAGGTTTGAC TGAGAAGAGC GTTCAGGAAG ACTGGCAACA TTTCCCAAGA
    TACAGAACAG CCTCTCAGGG GCCGCAGACA GACAGTGTCA TCCAGAACTC TGAAAATATT
    AAGGCCTATC ATTCTGGTGA AGGACATATG CCCTTTAGAA CTGGAACTGG TGACATTGCA
    ACTTGTTACA GTCCACGGAC TTCAACTGAA TCTTTTGCTC CACGGGATTC AGTGGGACTG
    GCACCCCAGG ATAGCCAGGC AAGTAACATT TTAGTAATGG ACCACATGAT TATGACCCCA
    GAGATGCCTA CCATGGAGCC AGAAGGGGGT CTTGATGACA GTGGAGAGCA CTTTTTTGAT
    GCCCGTGAAG CACATAGTGA TGAGAATCCA TCAGAAGGTG ATGGAGCAGT TAACAAGGAA
    GAGAAGGATG TTAATTTACG CATCTCAGGA AACTATTTGA TCCTTGATGG CTATGACCCA
    GTGCAGGAGA GTTCCACAGA TGAGGAGGTT GCTTCCTCAC TTACCCTGCA GCCCATGACA
    GGCATCCCTG CTGTGGAATC CACCCACCAG CAGCAACATT CTCCTCAGAA TACTCACTCC
    GATGGGGCAA TTTCACCATT CACCCCCGAA TTTCTGGTCC AGCAGCGCTG GGGAGCTATG
    GAGTATTCCT GTTTTGAGAT CCAGAGTCCC TCCTCTTGTG CAGATTCACA GAGCCAGATC
    ATGGAGTACA TTCATAAGAT AGAGGCTGAC CTTGAACACT TAAAGAAGGT GGAGGAAAGT
    TACACCATTC TTTGCCAAAG GCTGGCTGGA TCAGCCCTCA CAGACAAGCA CTCAGATAAA
    AGTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_016872911.1
    CDS472..4677
    Translation

    Target ORF information:

    RefSeq Version XM_017017422.2
    Organism Homo sapiens(human)
    Definition Homo sapiens Rho guanine nucleotide exchange factor 12 (ARHGEF12), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017017422.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    ATGCGGGCTG GAGTACAGAC AGGTGATCGA ATCATCAAGG TGAATGGAAC TCTGGTGACT 
    CATTCAAATC ATCTGGAGGT GGTGAAGCTA ATCAAATCTG GTTCCTATGT AGCTCTCACT
    GTTCAGGGAC GCCCACCTGG GTCGCCCCAG ATTCCACTTG CCGACTCTGA AGTAGAGCCG
    TCAGTCATTG GACATATGTC TCCCATCATG ACATCTCCTC ATTCACCTGG AGCATCTGGG
    AATATGGAGA GAATCACTAG TCCTGTGCTC ATGGGGGAGG AAAACAATGT GGTTCATAAC
    CAGAAAGTAG AAATTCTGAG AAAAATGTTA CAGAAAGAAC AGGAACGGCT ACAGGATGGA
    GCTGTAGTTA CACCCTCCAG ACCTTTAGGG GACACCCTAA CAGTCAGTGA GGCAGAAACA
    GATCCTGGAG ATGTACTGGG CAGGACTGAC TGTAGCAGTG GAGATGCTTC TCGGCCCAGT
    AGTGACAATG CAGATAGTCC CAAGAGTGGC CCAAAAGAGA GAATTTATCT AGAGGAAAAC
    CCAGAGAAAA GTGAAACAAT TCAGGACACT GACACTCAAT CACTTGTCGG AAGTCCCTCA
    ACCCGTATAG CACCTCATAT TATTGGAGCA GAAGATGATG ATTTTGGTAC TGAACATGAA
    CAGATCAATG GACAGTGCAG CTGTTTCCAG AGCATTGAAT TACTAAAATC TCGCCCGGCT
    CATTTGGCTG TTTTCTTACA CCATGTAGTT TCACAATTTG ACCCTGCGAC TTTGCTCTGT
    TATCTCTATT CAGACCTGTA TAAACATACC AATTCCAAAG AAACTCGTCG CATCTTCCTT
    GAGTTTCATC AGTTCTTTCT AGATCGATCA GCACACCTGA AAGTTTCTGT TCCTGATGAA
    ATGTCTGCAG ATCTAGAAAA GAGAAGACCT GAGCTCATTC CTGAGGATCT GCATCGCCAC
    TATATCCAAA CTATGCAAGA AAGAGTCCAT CCAGAAGTTC AAAGGCACTT AGAAGATTTT
    CGGCAGAAAC GTAGTATGGG ACTGACCTTG GCTGAAAGCG AGCTGACTAA ACTTGATGCA
    GAGCGAGACA AGGACCGATT GACTTTGGAG AAGGAGCGGA CATGTGCAGA ACAGATTGTT
    GCCAAAATTG AAGAAGTATT GATGACTGCT CAGGCTGTAG AGGAAGATAA GAGCTCCACC
    ATGCAGTATG TTATTCTCAT GTATATGAAG CATTTGGGAG TAAAAGTGAA AGAGCCTCGA
    AATTTGGAGC ACAAACGGGG TCGGATTGGA TTTCTTCCCA AAATCAAGCA AAGTATGAAG
    AAAGATAAAG AAGGGGAAGA AAAAGGGAAG CGAAGAGGAT TCCCCAGCAT CCTGGGACCC
    CCACGGAGAC CAAGCCGTCA TGACAACAGT GCAATTGGCA GAGCCATGGA ACTACAGAAG
    GCGCGCCACC CTAAGCACTT ATCCACACCC TCATCTGTGA GTCCTGAACC TCAGGACTCT
    GCCAAGTTGC GCCAGAGTGG GTTAGCAAAT GAAGGAACAG ACGCTGGATA CCTGCCTGCC
    AATTCCATGT CTTCTGTAGC TTCAGGGGCC TCTTTTTCCC AGGAAGGAGG GAAAGAGAAT
    GATACAGGAT CAAAGCAAGT TGGAGAAACA TCAGCACCTG GAGACACCTT AGATGGCACA
    CCTCGTACTC TCAATACTGT CTTTGATTTC CCACCACCTC CATTAGACCA AGTGCAGGAG
    GAGGAATGTG AAGTAGAAAG GGTGACTGAA CATGGGACAC CAAAGCCCTT TCGAAAGTTT
    GACAGTGTAG CTTTTGGAGA AAGTCAAAGT GAGGATGAAC AATTTGAAAA TGACTTAGAG
    ACAGATCCAC CCAACTGGCA GCAGCTTGTT AGTCGAGAAG TGTTACTGGG ACTAAAACCT
    TGTGAAATCA AAAGACAGGA AGTGATTAAT GAATTGTTCT ACACTGAAAG AGCTCATGTT
    CGAACACTGA AGGTTCTTGA TCAAGTGTTC TATCAGCGAG TATCCAGAGA AGGAATTCTG
    TCACCCTCAG AGCTACGGAA AATTTTTTCA AACTTGGAAG ATATTCTTCA ACTTCATATT
    GGATTGAATG AACAAATGAA GGCTGTTCGA AAGAGAAATG AGACCTCTGT TATCGATCAG
    ATTGGGGAAG ATTTGCTGAC ATGGTTCAGC GGACCAGGAG AGGAGAAATT GAAACATGCT
    GCTGCTACCT TTTGCAGTAA CCAACCTTTC GCCCTGGAAA TGATCAAATC TCGTCAGAAA
    AAGGATTCTC GATTTCAGAC TTTTGTGCAA GATGCTGAAA GTAATCCACT GTGTCGTCGT
    CTTCAACTGA AGGATATTAT TCCCACTCAA ATGCAAAGGC TTACTAAGTA CCCACTTCTG
    TTGGATAATA TTGCCAAATA CACAGAATGG CCAACAGAAA GGGAGAAGGT GAAGAAAGCT
    GCAGATCACT GTCGTCAGAT CTTAAATTAT GTAAATCAGG CTGTCAAGGA GGCAGAAAAC
    AAGCAGCGCC TAGAAGATTA TCAGCGTCGC CTTGATACCT CCAGCCTGAA GTTGTCAGAG
    TACCCAAATG TTGAAGAGCT CAGGAATTTG GATTTAACAA AAAGGAAGAT GATTCATGAA
    GGGCCATTGG TTTGGAAGGT GAATAGAGAT AAAACTATTG ATTTATACAC GTTGCTGCTG
    GAAGACATTC TTGTATTGTT ACAAAAGCAG GATGATAGAC TGGTTTTAAG GTGTCATAGT
    AAGATTCTGG CATCTACAGC TGATAGCAAA CACACGTTTA GCCCTGTCAT TAAGTTGAGT
    ACAGTGTTGG TTCGACAAGT GGCAACAGAT AACAAAGCTT TATTCGTCAT TTCCATGTCA
    GACAATGGCG CTCAGATTTA TGAACTGGTG GCACAGACAG TTTCTGAAAA GACTGTCTGG
    CAGGACCTAA TCTGTCGGAT GGCTGCATCA GTGAAGGAGC AATCCACAAA GCCAATTCCA
    TTACCACAGT CAACACCTGG CGAAGGAGAT AATGATGAAG AAGATCCTTC AAAATTAAAA
    GAGGAGCAGC ATGGCATTTC AGTCACTGGT TTGCAGAGTC CAGACAGAGA TTTGGGATTA
    GAATCTACCT TAATATCGTC AAAACCTCAG TCTCATTCAC TGAGTACCTC TGGGAAATCA
    GAGGTACGTG ATCTGTTTGT GGCTGAGAGA CAGTTTGCAA AGGAACAACA TACAGATGGG
    ACACTAAAGG AAGTTGGAGA AGATTATCAA ATCGCAATCC CAGATTCACA CCTGCCTGTC
    TCAGAAGAAC GGTGGGCATT GGATGCACTA AGAAATTTGG GTTTGTTGAA GCAGTTGCTG
    GTGCAACAGC TAGGTTTGAC TGAGAAGAGC GTTCAGGAAG ACTGGCAACA TTTCCCAAGA
    TACAGAACAG CCTCTCAGGG GCCGCAGACA GACAGTGTCA TCCAGAACTC TGAAAATATT
    AAGGCCTATC ATTCTGGTGA AGGACATATG CCCTTTAGAA CTGGAACTGG TGACATTGCA
    ACTTGTTACA GTCCACGGAC TTCAACTGAA TCTTTTGCTC CACGGGATTC AGTGGGACTG
    GCACCCCAGG ATAGCCAGGC AAGTAACATT TTAGTAATGG ACCACATGAT TATGACCCCA
    GAGATGCCTA CCATGGAGCC AGAAGGGGGT CTTGATGACA GTGGAGAGCA CTTTTTTGAT
    GCCCGTGAAG CACATAGTGA TGAGAATCCA TCAGAAGGTG ATGGAGCAGT TAACAAGGAA
    GAGAAGGATG TTAATTTACG CATCTCAGGA AACTATTTGA TCCTTGATGG CTATGACCCA
    GTGCAGGAGA GTTCCACAGA TGAGGAGGTT GCTTCCTCAC TTACCCTGCA GCCCATGACA
    GGCATCCCTG CTGTGGAATC CACCCACCAG CAGCAACATT CTCCTCAGAA TACTCACTCC
    GATGGGGCAA TTTCACCATT CACCCCCGAA TTTCTGGTCC AGCAGCGCTG GGGAGCTATG
    GAGTATTCCT GTTTTGAGAT CCAGAGTCCC TCCTCTTGTG CAGATTCACA GAGCCAGATC
    ATGGAGTACA TTCATAAGAT AGAGGCTGAC CTTGAACACT TAAAGAAGGT GGAGGAAAGT
    TACACCATTC TTTGCCAAAG GCTGGCTGGA TCAGCCCTCA CAGACAAGCA CTCAGATAAA
    AGTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu07698
    Clone ID Related Accession (Same CDS sequence) NM_001198665.1 , NM_001198665.2
    Accession Version NM_001198665.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4578bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-02-22
    Organism Homo sapiens(human)
    Product rho guanine nucleotide exchange factor 12 isoform 2
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BC063117.1, AF180681.1, AK304316.1 and AP000758.5. Transcript Variant: This variant (2) lacks an alternate in-frame exon compared to variant 1. The resulting isoform (2) has the same N- and C-termini but is shorter compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    ATGAGTGGCA CACAGTCTAC TATCACCGAC AGGTTTCCCC TCAAAAAACC TATAAGGCAT 
    GGAAGTATTT TGAACCGAGA GTCACCAACA GATAAGAAGC AGAAAGTTGA GCGCATTGCA
    TCACATGATT TTGACCCCAC AGGTCTTGTT CAGCGTTGCG TAATCATCCA GAAAGATGAC
    AATGGATTTG GGCTGACGGT CAGTGGAGAC AATCCAGTCT TCGTACAGTC TGTCAAAGAA
    GATGGAGCAG CCATGCGGGC TGGAGTACAG ACAGGTGATC GAATCATCAA GGTGAATGGA
    ACTCTGGTGA CTCATTCAAA TCATCTGGAG GTGGTGAAGC TAATCAAATC TGGTTCCTAT
    GTAGCTCTCA CTGTTCAGGG ACGCCCACCT GGGTCGCCCC AGATTCCACT TGCCGACTCT
    GAAGTAGAGC CGTCAGTCAT TGGACATATG TCTCCCATCA TGACATCTCC TCATTCACCT
    GGAGCATCTG GGAATATGGA GAGAATCACT AGTCCTGTGC TCATGGGGGA GGAAAACAAT
    GTGGTTCATA ACCAGAAAGT AGAAATTCTG AGAAAAATGT TACAGAAAGA ACAGGAACGG
    CTACAGTTAT TGCAGGAAGA TTACAACCGA ACACCTGCCC AAAGATTGCT AAAAGAGATC
    CAAGAGGCCA AGAAACACAT TCCTCAGCTG CAAGAGCAGT TATCCAAAGC CACAGGCTCT
    GCTCAGGATG GAGCTGTAGT TACACCCTCC AGACCTTTAG GGGACACCCT AACAGTCAGT
    GAGGCAGAAA CAGATCCTGG AGATGTACTG GGCAGGACTG ACTGTAGCAG TGGAGATGCT
    TCTCGGCCCA GTAGTGACAA TGCAGATAGT CCCAAGAGTG GCCCAAAAGA GAGAATTTAT
    CTAGAGGAAA ACCCAGAGAA AAGTGAAACA ATTCAGGACA CTGACACTCA ATCACTTGTC
    GGAAGTCCCT CAACCCGTAT AGCACCTCAT ATTATTGGAG CAGAAGATGA TGATTTTGGT
    ACTGAACATG AACAGATCAA TGGACAGTGC AGCTGTTTCC AGAGCATTGA ATTACTAAAA
    TCTCGCCCGG CTCATTTGGC TGTTTTCTTA CACCATGTAG TTTCACAATT TGACCCTGCG
    ACTTTGCTCT GTTATCTCTA TTCAGACCTG TATAAACATA CCAATTCCAA AGAAACTCGT
    CGCATCTTCC TTGAGTTTCA TCAGTTCTTT CTAGATCGAT CAGCACACCT GAAAGTTTCT
    GTTCCTGATG AAATGTCTGC AGATCTAGAA AAGAGAAGAC CTGAGCTCAT TCCTGAGGAT
    CTGCATCGCC ACTATATCCA AACTATGCAA GAAAGAGTCC ATCCAGAAGT TCAAAGGCAC
    TTAGAAGATT TTCGGCAGAA ACGTAGTATG GGACTGACCT TGGCTGAAAG CGAGCTGACT
    AAACTTGATG CAGAGCGAGA CAAGGACCGA TTGACTTTGG AGAAGGAGCG GACATGTGCA
    GAACAGATTG TTGCCAAAAT TGAAGAAGTA TTGATGACTG CTCAGGCTGT AGAGGAAGAT
    AAGAGCTCCA CCATGCAGTA TGTTATTCTC ATGTATATGA AGCATTTGGG AGTAAAAGTG
    AAAGAGCCTC GAAATTTGGA GCACAAACGG GGTCGGATTG GATTTCTTCC CAAAATCAAG
    CAAAGTATGA AGAAAGATAA AGAAGGGGAA GAAAAAGGGA AGCGAAGAGG ATTCCCCAGC
    ATCCTGGGAC CCCCACGGAG ACCAAGCCGT CATGACAACA GTGCAATTGG CAGAGCCATG
    GAACTACAGA AGGCGCGCCA CCCTAAGCAC TTATCCACAC CCTCATCTGT GAGTCCTGAA
    CCTCAGGACT CTGCCAAGTT GCGCCAGAGT GGGTTAGCAA ATGAAGGAAC AGACGCTGGA
    TACCTGCCTG CCAATTCCAT GTCTTCTGTA GCTTCAGGGG CCTCTTTTTC CCAGGAAGGA
    GGGAAAGAGA ATGATACAGG ATCAAAGCAA GTTGGAGAAA CATCAGCACC TGGAGACACC
    TTAGATGGCA CACCTCGTAC TCTCAATACT GTCTTTGATT TCCCACCACC TCCATTAGAC
    CAAGTGCAGG AGGAGGAATG TGAAGTAGAA AGGGTGACTG AACATGGGAC ACCAAAGCCC
    TTTCGAAAGT TTGACAGTGT AGCTTTTGGA GAAAGTCAAA GTGAGGATGA ACAATTTGAA
    AATGACTTAG AGACAGATCC ACCCAACTGG CAGCAGCTTG TTAGTCGAGA AGTGTTACTG
    GGACTAAAAC CTTGTGAAAT CAAAAGACAG GAAGTGATTA ATGAATTGTT CTACACTGAA
    AGAGCTCATG TTCGAACACT GAAGGTTCTT GATCAAGTGT TCTATCAGCG AGTATCCAGA
    GAAGGAATTC TGTCACCCTC AGAGCTACGG AAAATTTTTT CAAACTTGGA AGATATTCTT
    CAACTTCATA TTGGATTGAA TGAACAAATG AAGGCTGTTC GAAAGAGAAA TGAGACCTCT
    GTTATCGATC AGATTGGGGA AGATTTGCTG ACATGGTTCA GCGGACCAGG AGAGGAGAAA
    TTGAAACATG CTGCTGCTAC CTTTTGCAGT AACCAACCTT TCGCCCTGGA AATGATCAAA
    TCTCGTCAGA AAAAGGATTC TCGATTTCAG ACTTTTGTGC AAGATGCTGA AAGTAATCCA
    CTGTGTCGTC GTCTTCAACT GAAGGATATT ATTCCCACTC AAATGCAAAG GCTTACTAAG
    TACCCACTTC TGTTGGATAA TATTGCCAAA TACACAGAAT GGCCAACAGA AAGGGAGAAG
    GTGAAGAAAG CTGCAGATCA CTGTCGTCAG ATCTTAAATT ATGTAAATCA GGCTGTCAAG
    GAGGCAGAAA ACAAGCAGCG CCTAGAAGAT TATCAGCGTC GCCTTGATAC CTCCAGCCTG
    AAGTTGTCAG AGTACCCAAA TGTTGAAGAG CTCAGGAATT TGGATTTAAC AAAAAGGAAG
    ATGATTCATG AAGGGCCATT GGTTTGGAAG GTGAATAGAG ATAAAACTAT TGATTTATAC
    ACGTTGCTGC TGGAAGACAT TCTTGTATTG TTACAAAAGC AGGATGATAG ACTGGTTTTA
    AGGTGTCATA GTAAGATTCT GGCATCTACA GCTGATAGCA AACACACGTT TAGCCCTGTC
    ATTAAGTTGA GTACAGTGTT GGTTCGACAA GTGGCAACAG ATAACAAAGC TTTATTCGTC
    ATTTCCATGT CAGACAATGG CGCTCAGATT TATGAACTGG TGGCACAGAC AGTTTCTGAA
    AAGACTGTCT GGCAGGACCT AATCTGTCGG ATGGCTGCAT CAGTGAAGGA GCAATCCACA
    AAGCCAATTC CATTACCACA GTCAACACCT GGCGAAGGAG ATAATGATGA AGAAGATCCT
    TCAAAATTAA AAGAGGAGCA GCATGGCATT TCAGTCACTG GTTTGCAGAG TCCAGACAGA
    GATTTGGGAT TAGAATCTAC CTTAATATCG TCAAAACCTC AGTCTCATTC ACTGAGTACC
    TCTGGGAAAT CAGAGGTACG TGATCTGTTT GTGGCTGAGA GACAGTTTGC AAAGGAACAA
    CATACAGATG GGACACTAAA GGAAGTTGGA GAAGATTATC AAATCGCAAT CCCAGATTCA
    CACCTGCCTG TCTCAGAAGA ACGGTGGGCA TTGGATGCAC TAAGAAATTT GGGTTTGTTG
    AAGCAGTTGC TGGTGCAACA GCTAGGTTTG ACTGAGAAGA GCGTTCAGGA AGACTGGCAA
    CATTTCCCAA GATACAGAAC AGCCTCTCAG GGGCCGCAGA CAGACAGTGT CATCCAGAAC
    TCTGAAAATA TTAAGGCCTA TCATTCTGGT GAAGGACATA TGCCCTTTAG AACTGGAACT
    GGTGACATTG CAACTTGTTA CAGTCCACGG ACTTCAACTG AATCTTTTGC TCCACGGGAT
    TCAGTGGGAC TGGCACCCCA GGATAGCCAG GCAAGTAACA TTTTAGTAAT GGACCACATG
    ATTATGACCC CAGAGATGCC TACCATGGAG CCAGAAGGGG GTCTTGATGA CAGTGGAGAG
    CACTTTTTTG ATGCCCGTGA AGCACATAGT GATGAGAATC CATCAGAAGG TGATGGAGCA
    GTTAACAAGG AAGAGAAGGA TGTTAATTTA CGCATCTCAG GAAACTATTT GATCCTTGAT
    GGCTATGACC CAGTGCAGGA GAGTTCCACA GATGAGGAGG TTGCTTCCTC ACTTACCCTG
    CAGCCCATGA CAGGCATCCC TGCTGTGGAA TCCACCCACC AGCAGCAACA TTCTCCTCAG
    AATACTCACT CCGATGGGGC AATTTCACCA TTCACCCCCG AATTTCTGGT CCAGCAGCGC
    TGGGGAGCTA TGGAGTATTC CTGTTTTGAG ATCCAGAGTC CCTCCTCTTG TGCAGATTCA
    CAGAGCCAGA TCATGGAGTA CATTCATAAG ATAGAGGCTG ACCTTGAACA CTTAAAGAAG
    GTGGAGGAAA GTTACACCAT TCTTTGCCAA AGGCTGGCTG GATCAGCCCT CACAGACAAG
    CACTCAGATA AAAGTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001185594.1
    CDS336..4913
    Translation

    Target ORF information:

    RefSeq Version NM_001198665.1
    Organism Homo sapiens(human)
    Definition Homo sapiens Rho guanine nucleotide exchange factor 12 (ARHGEF12), transcript variant 2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001198665.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    ATGAGTGGCA CACAGTCTAC TATCACCGAC AGGTTTCCCC TCAAAAAACC TATAAGGCAT 
    GGAAGTATTT TGAACCGAGA GTCACCAACA GATAAGAAGC AGAAAGTTGA GCGCATTGCA
    TCACATGATT TTGACCCCAC AGGTCTTGTT CAGCGTTGCG TAATCATCCA GAAAGATGAC
    AATGGATTTG GGCTGACGGT CAGTGGAGAC AATCCAGTCT TCGTACAGTC TGTCAAAGAA
    GATGGAGCAG CCATGCGGGC TGGAGTACAG ACAGGTGATC GAATCATCAA GGTGAATGGA
    ACTCTGGTGA CTCATTCAAA TCATCTGGAG GTGGTGAAGC TAATCAAATC TGGTTCCTAT
    GTAGCTCTCA CTGTTCAGGG ACGCCCACCT GGGTCGCCCC AGATTCCACT TGCCGACTCT
    GAAGTAGAGC CGTCAGTCAT TGGACATATG TCTCCCATCA TGACATCTCC TCATTCACCT
    GGAGCATCTG GGAATATGGA GAGAATCACT AGTCCTGTGC TCATGGGGGA GGAAAACAAT
    GTGGTTCATA ACCAGAAAGT AGAAATTCTG AGAAAAATGT TACAGAAAGA ACAGGAACGG
    CTACAGTTAT TGCAGGAAGA TTACAACCGA ACACCTGCCC AAAGATTGCT AAAAGAGATC
    CAAGAGGCCA AGAAACACAT TCCTCAGCTG CAAGAGCAGT TATCCAAAGC CACAGGCTCT
    GCTCAGGATG GAGCTGTAGT TACACCCTCC AGACCTTTAG GGGACACCCT AACAGTCAGT
    GAGGCAGAAA CAGATCCTGG AGATGTACTG GGCAGGACTG ACTGTAGCAG TGGAGATGCT
    TCTCGGCCCA GTAGTGACAA TGCAGATAGT CCCAAGAGTG GCCCAAAAGA GAGAATTTAT
    CTAGAGGAAA ACCCAGAGAA AAGTGAAACA ATTCAGGACA CTGACACTCA ATCACTTGTC
    GGAAGTCCCT CAACCCGTAT AGCACCTCAT ATTATTGGAG CAGAAGATGA TGATTTTGGT
    ACTGAACATG AACAGATCAA TGGACAGTGC AGCTGTTTCC AGAGCATTGA ATTACTAAAA
    TCTCGCCCGG CTCATTTGGC TGTTTTCTTA CACCATGTAG TTTCACAATT TGACCCTGCG
    ACTTTGCTCT GTTATCTCTA TTCAGACCTG TATAAACATA CCAATTCCAA AGAAACTCGT
    CGCATCTTCC TTGAGTTTCA TCAGTTCTTT CTAGATCGAT CAGCACACCT GAAAGTTTCT
    GTTCCTGATG AAATGTCTGC AGATCTAGAA AAGAGAAGAC CTGAGCTCAT TCCTGAGGAT
    CTGCATCGCC ACTATATCCA AACTATGCAA GAAAGAGTCC ATCCAGAAGT TCAAAGGCAC
    TTAGAAGATT TTCGGCAGAA ACGTAGTATG GGACTGACCT TGGCTGAAAG CGAGCTGACT
    AAACTTGATG CAGAGCGAGA CAAGGACCGA TTGACTTTGG AGAAGGAGCG GACATGTGCA
    GAACAGATTG TTGCCAAAAT TGAAGAAGTA TTGATGACTG CTCAGGCTGT AGAGGAAGAT
    AAGAGCTCCA CCATGCAGTA TGTTATTCTC ATGTATATGA AGCATTTGGG AGTAAAAGTG
    AAAGAGCCTC GAAATTTGGA GCACAAACGG GGTCGGATTG GATTTCTTCC CAAAATCAAG
    CAAAGTATGA AGAAAGATAA AGAAGGGGAA GAAAAAGGGA AGCGAAGAGG ATTCCCCAGC
    ATCCTGGGAC CCCCACGGAG ACCAAGCCGT CATGACAACA GTGCAATTGG CAGAGCCATG
    GAACTACAGA AGGCGCGCCA CCCTAAGCAC TTATCCACAC CCTCATCTGT GAGTCCTGAA
    CCTCAGGACT CTGCCAAGTT GCGCCAGAGT GGGTTAGCAA ATGAAGGAAC AGACGCTGGA
    TACCTGCCTG CCAATTCCAT GTCTTCTGTA GCTTCAGGGG CCTCTTTTTC CCAGGAAGGA
    GGGAAAGAGA ATGATACAGG ATCAAAGCAA GTTGGAGAAA CATCAGCACC TGGAGACACC
    TTAGATGGCA CACCTCGTAC TCTCAATACT GTCTTTGATT TCCCACCACC TCCATTAGAC
    CAAGTGCAGG AGGAGGAATG TGAAGTAGAA AGGGTGACTG AACATGGGAC ACCAAAGCCC
    TTTCGAAAGT TTGACAGTGT AGCTTTTGGA GAAAGTCAAA GTGAGGATGA ACAATTTGAA
    AATGACTTAG AGACAGATCC ACCCAACTGG CAGCAGCTTG TTAGTCGAGA AGTGTTACTG
    GGACTAAAAC CTTGTGAAAT CAAAAGACAG GAAGTGATTA ATGAATTGTT CTACACTGAA
    AGAGCTCATG TTCGAACACT GAAGGTTCTT GATCAAGTGT TCTATCAGCG AGTATCCAGA
    GAAGGAATTC TGTCACCCTC AGAGCTACGG AAAATTTTTT CAAACTTGGA AGATATTCTT
    CAACTTCATA TTGGATTGAA TGAACAAATG AAGGCTGTTC GAAAGAGAAA TGAGACCTCT
    GTTATCGATC AGATTGGGGA AGATTTGCTG ACATGGTTCA GCGGACCAGG AGAGGAGAAA
    TTGAAACATG CTGCTGCTAC CTTTTGCAGT AACCAACCTT TCGCCCTGGA AATGATCAAA
    TCTCGTCAGA AAAAGGATTC TCGATTTCAG ACTTTTGTGC AAGATGCTGA AAGTAATCCA
    CTGTGTCGTC GTCTTCAACT GAAGGATATT ATTCCCACTC AAATGCAAAG GCTTACTAAG
    TACCCACTTC TGTTGGATAA TATTGCCAAA TACACAGAAT GGCCAACAGA AAGGGAGAAG
    GTGAAGAAAG CTGCAGATCA CTGTCGTCAG ATCTTAAATT ATGTAAATCA GGCTGTCAAG
    GAGGCAGAAA ACAAGCAGCG CCTAGAAGAT TATCAGCGTC GCCTTGATAC CTCCAGCCTG
    AAGTTGTCAG AGTACCCAAA TGTTGAAGAG CTCAGGAATT TGGATTTAAC AAAAAGGAAG
    ATGATTCATG AAGGGCCATT GGTTTGGAAG GTGAATAGAG ATAAAACTAT TGATTTATAC
    ACGTTGCTGC TGGAAGACAT TCTTGTATTG TTACAAAAGC AGGATGATAG ACTGGTTTTA
    AGGTGTCATA GTAAGATTCT GGCATCTACA GCTGATAGCA AACACACGTT TAGCCCTGTC
    ATTAAGTTGA GTACAGTGTT GGTTCGACAA GTGGCAACAG ATAACAAAGC TTTATTCGTC
    ATTTCCATGT CAGACAATGG CGCTCAGATT TATGAACTGG TGGCACAGAC AGTTTCTGAA
    AAGACTGTCT GGCAGGACCT AATCTGTCGG ATGGCTGCAT CAGTGAAGGA GCAATCCACA
    AAGCCAATTC CATTACCACA GTCAACACCT GGCGAAGGAG ATAATGATGA AGAAGATCCT
    TCAAAATTAA AAGAGGAGCA GCATGGCATT TCAGTCACTG GTTTGCAGAG TCCAGACAGA
    GATTTGGGAT TAGAATCTAC CTTAATATCG TCAAAACCTC AGTCTCATTC ACTGAGTACC
    TCTGGGAAAT CAGAGGTACG TGATCTGTTT GTGGCTGAGA GACAGTTTGC AAAGGAACAA
    CATACAGATG GGACACTAAA GGAAGTTGGA GAAGATTATC AAATCGCAAT CCCAGATTCA
    CACCTGCCTG TCTCAGAAGA ACGGTGGGCA TTGGATGCAC TAAGAAATTT GGGTTTGTTG
    AAGCAGTTGC TGGTGCAACA GCTAGGTTTG ACTGAGAAGA GCGTTCAGGA AGACTGGCAA
    CATTTCCCAA GATACAGAAC AGCCTCTCAG GGGCCGCAGA CAGACAGTGT CATCCAGAAC
    TCTGAAAATA TTAAGGCCTA TCATTCTGGT GAAGGACATA TGCCCTTTAG AACTGGAACT
    GGTGACATTG CAACTTGTTA CAGTCCACGG ACTTCAACTG AATCTTTTGC TCCACGGGAT
    TCAGTGGGAC TGGCACCCCA GGATAGCCAG GCAAGTAACA TTTTAGTAAT GGACCACATG
    ATTATGACCC CAGAGATGCC TACCATGGAG CCAGAAGGGG GTCTTGATGA CAGTGGAGAG
    CACTTTTTTG ATGCCCGTGA AGCACATAGT GATGAGAATC CATCAGAAGG TGATGGAGCA
    GTTAACAAGG AAGAGAAGGA TGTTAATTTA CGCATCTCAG GAAACTATTT GATCCTTGAT
    GGCTATGACC CAGTGCAGGA GAGTTCCACA GATGAGGAGG TTGCTTCCTC ACTTACCCTG
    CAGCCCATGA CAGGCATCCC TGCTGTGGAA TCCACCCACC AGCAGCAACA TTCTCCTCAG
    AATACTCACT CCGATGGGGC AATTTCACCA TTCACCCCCG AATTTCTGGT CCAGCAGCGC
    TGGGGAGCTA TGGAGTATTC CTGTTTTGAG ATCCAGAGTC CCTCCTCTTG TGCAGATTCA
    CAGAGCCAGA TCATGGAGTA CATTCATAAG ATAGAGGCTG ACCTTGAACA CTTAAAGAAG
    GTGGAGGAAA GTTACACCAT TCTTTGCCAA AGGCTGGCTG GATCAGCCCT CACAGACAAG
    CACTCAGATA AAAGTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu07698
    Clone ID Related Accession (Same CDS sequence) NM_001198665.1 , NM_001198665.2
    Accession Version NM_001198665.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4578bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-10-25
    Organism Homo sapiens(human)
    Product rho guanine nucleotide exchange factor 12 isoform 2
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AP001150.5, BC063117.1, AF180681.1, AK304316.1 and AP000758.5. On Jun 1, 2019 this sequence version replaced NM_001198665.1. Transcript Variant: This variant (2) lacks an alternate in-frame exon compared to variant 1. The resulting isoform (2) has the same N- and C-termini but is shorter compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    ATGAGTGGCA CACAGTCTAC TATCACCGAC AGGTTTCCCC TCAAAAAACC TATAAGGCAT 
    GGAAGTATTT TGAACCGAGA GTCACCAACA GATAAGAAGC AGAAAGTTGA GCGCATTGCA
    TCACATGATT TTGACCCCAC AGGTCTTGTT CAGCGTTGCG TAATCATCCA GAAAGATGAC
    AATGGATTTG GGCTGACGGT CAGTGGAGAC AATCCAGTCT TCGTACAGTC TGTCAAAGAA
    GATGGAGCAG CCATGCGGGC TGGAGTACAG ACAGGTGATC GAATCATCAA GGTGAATGGA
    ACTCTGGTGA CTCATTCAAA TCATCTGGAG GTGGTGAAGC TAATCAAATC TGGTTCCTAT
    GTAGCTCTCA CTGTTCAGGG ACGCCCACCT GGGTCGCCCC AGATTCCACT TGCCGACTCT
    GAAGTAGAGC CGTCAGTCAT TGGACATATG TCTCCCATCA TGACATCTCC TCATTCACCT
    GGAGCATCTG GGAATATGGA GAGAATCACT AGTCCTGTGC TCATGGGGGA GGAAAACAAT
    GTGGTTCATA ACCAGAAAGT AGAAATTCTG AGAAAAATGT TACAGAAAGA ACAGGAACGG
    CTACAGTTAT TGCAGGAAGA TTACAACCGA ACACCTGCCC AAAGATTGCT AAAAGAGATC
    CAAGAGGCCA AGAAACACAT TCCTCAGCTG CAAGAGCAGT TATCCAAAGC CACAGGCTCT
    GCTCAGGATG GAGCTGTAGT TACACCCTCC AGACCTTTAG GGGACACCCT AACAGTCAGT
    GAGGCAGAAA CAGATCCTGG AGATGTACTG GGCAGGACTG ACTGTAGCAG TGGAGATGCT
    TCTCGGCCCA GTAGTGACAA TGCAGATAGT CCCAAGAGTG GCCCAAAAGA GAGAATTTAT
    CTAGAGGAAA ACCCAGAGAA AAGTGAAACA ATTCAGGACA CTGACACTCA ATCACTTGTC
    GGAAGTCCCT CAACCCGTAT AGCACCTCAT ATTATTGGAG CAGAAGATGA TGATTTTGGT
    ACTGAACATG AACAGATCAA TGGACAGTGC AGCTGTTTCC AGAGCATTGA ATTACTAAAA
    TCTCGCCCGG CTCATTTGGC TGTTTTCTTA CACCATGTAG TTTCACAATT TGACCCTGCG
    ACTTTGCTCT GTTATCTCTA TTCAGACCTG TATAAACATA CCAATTCCAA AGAAACTCGT
    CGCATCTTCC TTGAGTTTCA TCAGTTCTTT CTAGATCGAT CAGCACACCT GAAAGTTTCT
    GTTCCTGATG AAATGTCTGC AGATCTAGAA AAGAGAAGAC CTGAGCTCAT TCCTGAGGAT
    CTGCATCGCC ACTATATCCA AACTATGCAA GAAAGAGTCC ATCCAGAAGT TCAAAGGCAC
    TTAGAAGATT TTCGGCAGAA ACGTAGTATG GGACTGACCT TGGCTGAAAG CGAGCTGACT
    AAACTTGATG CAGAGCGAGA CAAGGACCGA TTGACTTTGG AGAAGGAGCG GACATGTGCA
    GAACAGATTG TTGCCAAAAT TGAAGAAGTA TTGATGACTG CTCAGGCTGT AGAGGAAGAT
    AAGAGCTCCA CCATGCAGTA TGTTATTCTC ATGTATATGA AGCATTTGGG AGTAAAAGTG
    AAAGAGCCTC GAAATTTGGA GCACAAACGG GGTCGGATTG GATTTCTTCC CAAAATCAAG
    CAAAGTATGA AGAAAGATAA AGAAGGGGAA GAAAAAGGGA AGCGAAGAGG ATTCCCCAGC
    ATCCTGGGAC CCCCACGGAG ACCAAGCCGT CATGACAACA GTGCAATTGG CAGAGCCATG
    GAACTACAGA AGGCGCGCCA CCCTAAGCAC TTATCCACAC CCTCATCTGT GAGTCCTGAA
    CCTCAGGACT CTGCCAAGTT GCGCCAGAGT GGGTTAGCAA ATGAAGGAAC AGACGCTGGA
    TACCTGCCTG CCAATTCCAT GTCTTCTGTA GCTTCAGGGG CCTCTTTTTC CCAGGAAGGA
    GGGAAAGAGA ATGATACAGG ATCAAAGCAA GTTGGAGAAA CATCAGCACC TGGAGACACC
    TTAGATGGCA CACCTCGTAC TCTCAATACT GTCTTTGATT TCCCACCACC TCCATTAGAC
    CAAGTGCAGG AGGAGGAATG TGAAGTAGAA AGGGTGACTG AACATGGGAC ACCAAAGCCC
    TTTCGAAAGT TTGACAGTGT AGCTTTTGGA GAAAGTCAAA GTGAGGATGA ACAATTTGAA
    AATGACTTAG AGACAGATCC ACCCAACTGG CAGCAGCTTG TTAGTCGAGA AGTGTTACTG
    GGACTAAAAC CTTGTGAAAT CAAAAGACAG GAAGTGATTA ATGAATTGTT CTACACTGAA
    AGAGCTCATG TTCGAACACT GAAGGTTCTT GATCAAGTGT TCTATCAGCG AGTATCCAGA
    GAAGGAATTC TGTCACCCTC AGAGCTACGG AAAATTTTTT CAAACTTGGA AGATATTCTT
    CAACTTCATA TTGGATTGAA TGAACAAATG AAGGCTGTTC GAAAGAGAAA TGAGACCTCT
    GTTATCGATC AGATTGGGGA AGATTTGCTG ACATGGTTCA GCGGACCAGG AGAGGAGAAA
    TTGAAACATG CTGCTGCTAC CTTTTGCAGT AACCAACCTT TCGCCCTGGA AATGATCAAA
    TCTCGTCAGA AAAAGGATTC TCGATTTCAG ACTTTTGTGC AAGATGCTGA AAGTAATCCA
    CTGTGTCGTC GTCTTCAACT GAAGGATATT ATTCCCACTC AAATGCAAAG GCTTACTAAG
    TACCCACTTC TGTTGGATAA TATTGCCAAA TACACAGAAT GGCCAACAGA AAGGGAGAAG
    GTGAAGAAAG CTGCAGATCA CTGTCGTCAG ATCTTAAATT ATGTAAATCA GGCTGTCAAG
    GAGGCAGAAA ACAAGCAGCG CCTAGAAGAT TATCAGCGTC GCCTTGATAC CTCCAGCCTG
    AAGTTGTCAG AGTACCCAAA TGTTGAAGAG CTCAGGAATT TGGATTTAAC AAAAAGGAAG
    ATGATTCATG AAGGGCCATT GGTTTGGAAG GTGAATAGAG ATAAAACTAT TGATTTATAC
    ACGTTGCTGC TGGAAGACAT TCTTGTATTG TTACAAAAGC AGGATGATAG ACTGGTTTTA
    AGGTGTCATA GTAAGATTCT GGCATCTACA GCTGATAGCA AACACACGTT TAGCCCTGTC
    ATTAAGTTGA GTACAGTGTT GGTTCGACAA GTGGCAACAG ATAACAAAGC TTTATTCGTC
    ATTTCCATGT CAGACAATGG CGCTCAGATT TATGAACTGG TGGCACAGAC AGTTTCTGAA
    AAGACTGTCT GGCAGGACCT AATCTGTCGG ATGGCTGCAT CAGTGAAGGA GCAATCCACA
    AAGCCAATTC CATTACCACA GTCAACACCT GGCGAAGGAG ATAATGATGA AGAAGATCCT
    TCAAAATTAA AAGAGGAGCA GCATGGCATT TCAGTCACTG GTTTGCAGAG TCCAGACAGA
    GATTTGGGAT TAGAATCTAC CTTAATATCG TCAAAACCTC AGTCTCATTC ACTGAGTACC
    TCTGGGAAAT CAGAGGTACG TGATCTGTTT GTGGCTGAGA GACAGTTTGC AAAGGAACAA
    CATACAGATG GGACACTAAA GGAAGTTGGA GAAGATTATC AAATCGCAAT CCCAGATTCA
    CACCTGCCTG TCTCAGAAGA ACGGTGGGCA TTGGATGCAC TAAGAAATTT GGGTTTGTTG
    AAGCAGTTGC TGGTGCAACA GCTAGGTTTG ACTGAGAAGA GCGTTCAGGA AGACTGGCAA
    CATTTCCCAA GATACAGAAC AGCCTCTCAG GGGCCGCAGA CAGACAGTGT CATCCAGAAC
    TCTGAAAATA TTAAGGCCTA TCATTCTGGT GAAGGACATA TGCCCTTTAG AACTGGAACT
    GGTGACATTG CAACTTGTTA CAGTCCACGG ACTTCAACTG AATCTTTTGC TCCACGGGAT
    TCAGTGGGAC TGGCACCCCA GGATAGCCAG GCAAGTAACA TTTTAGTAAT GGACCACATG
    ATTATGACCC CAGAGATGCC TACCATGGAG CCAGAAGGGG GTCTTGATGA CAGTGGAGAG
    CACTTTTTTG ATGCCCGTGA AGCACATAGT GATGAGAATC CATCAGAAGG TGATGGAGCA
    GTTAACAAGG AAGAGAAGGA TGTTAATTTA CGCATCTCAG GAAACTATTT GATCCTTGAT
    GGCTATGACC CAGTGCAGGA GAGTTCCACA GATGAGGAGG TTGCTTCCTC ACTTACCCTG
    CAGCCCATGA CAGGCATCCC TGCTGTGGAA TCCACCCACC AGCAGCAACA TTCTCCTCAG
    AATACTCACT CCGATGGGGC AATTTCACCA TTCACCCCCG AATTTCTGGT CCAGCAGCGC
    TGGGGAGCTA TGGAGTATTC CTGTTTTGAG ATCCAGAGTC CCTCCTCTTG TGCAGATTCA
    CAGAGCCAGA TCATGGAGTA CATTCATAAG ATAGAGGCTG ACCTTGAACA CTTAAAGAAG
    GTGGAGGAAA GTTACACCAT TCTTTGCCAA AGGCTGGCTG GATCAGCCCT CACAGACAAG
    CACTCAGATA AAAGTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001185594.1
    CDS832..5409
    Translation

    Target ORF information:

    RefSeq Version NM_001198665.2
    Organism Homo sapiens(human)
    Definition Homo sapiens Rho guanine nucleotide exchange factor 12 (ARHGEF12), transcript variant 2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001198665.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    ATGAGTGGCA CACAGTCTAC TATCACCGAC AGGTTTCCCC TCAAAAAACC TATAAGGCAT 
    GGAAGTATTT TGAACCGAGA GTCACCAACA GATAAGAAGC AGAAAGTTGA GCGCATTGCA
    TCACATGATT TTGACCCCAC AGGTCTTGTT CAGCGTTGCG TAATCATCCA GAAAGATGAC
    AATGGATTTG GGCTGACGGT CAGTGGAGAC AATCCAGTCT TCGTACAGTC TGTCAAAGAA
    GATGGAGCAG CCATGCGGGC TGGAGTACAG ACAGGTGATC GAATCATCAA GGTGAATGGA
    ACTCTGGTGA CTCATTCAAA TCATCTGGAG GTGGTGAAGC TAATCAAATC TGGTTCCTAT
    GTAGCTCTCA CTGTTCAGGG ACGCCCACCT GGGTCGCCCC AGATTCCACT TGCCGACTCT
    GAAGTAGAGC CGTCAGTCAT TGGACATATG TCTCCCATCA TGACATCTCC TCATTCACCT
    GGAGCATCTG GGAATATGGA GAGAATCACT AGTCCTGTGC TCATGGGGGA GGAAAACAAT
    GTGGTTCATA ACCAGAAAGT AGAAATTCTG AGAAAAATGT TACAGAAAGA ACAGGAACGG
    CTACAGTTAT TGCAGGAAGA TTACAACCGA ACACCTGCCC AAAGATTGCT AAAAGAGATC
    CAAGAGGCCA AGAAACACAT TCCTCAGCTG CAAGAGCAGT TATCCAAAGC CACAGGCTCT
    GCTCAGGATG GAGCTGTAGT TACACCCTCC AGACCTTTAG GGGACACCCT AACAGTCAGT
    GAGGCAGAAA CAGATCCTGG AGATGTACTG GGCAGGACTG ACTGTAGCAG TGGAGATGCT
    TCTCGGCCCA GTAGTGACAA TGCAGATAGT CCCAAGAGTG GCCCAAAAGA GAGAATTTAT
    CTAGAGGAAA ACCCAGAGAA AAGTGAAACA ATTCAGGACA CTGACACTCA ATCACTTGTC
    GGAAGTCCCT CAACCCGTAT AGCACCTCAT ATTATTGGAG CAGAAGATGA TGATTTTGGT
    ACTGAACATG AACAGATCAA TGGACAGTGC AGCTGTTTCC AGAGCATTGA ATTACTAAAA
    TCTCGCCCGG CTCATTTGGC TGTTTTCTTA CACCATGTAG TTTCACAATT TGACCCTGCG
    ACTTTGCTCT GTTATCTCTA TTCAGACCTG TATAAACATA CCAATTCCAA AGAAACTCGT
    CGCATCTTCC TTGAGTTTCA TCAGTTCTTT CTAGATCGAT CAGCACACCT GAAAGTTTCT
    GTTCCTGATG AAATGTCTGC AGATCTAGAA AAGAGAAGAC CTGAGCTCAT TCCTGAGGAT
    CTGCATCGCC ACTATATCCA AACTATGCAA GAAAGAGTCC ATCCAGAAGT TCAAAGGCAC
    TTAGAAGATT TTCGGCAGAA ACGTAGTATG GGACTGACCT TGGCTGAAAG CGAGCTGACT
    AAACTTGATG CAGAGCGAGA CAAGGACCGA TTGACTTTGG AGAAGGAGCG GACATGTGCA
    GAACAGATTG TTGCCAAAAT TGAAGAAGTA TTGATGACTG CTCAGGCTGT AGAGGAAGAT
    AAGAGCTCCA CCATGCAGTA TGTTATTCTC ATGTATATGA AGCATTTGGG AGTAAAAGTG
    AAAGAGCCTC GAAATTTGGA GCACAAACGG GGTCGGATTG GATTTCTTCC CAAAATCAAG
    CAAAGTATGA AGAAAGATAA AGAAGGGGAA GAAAAAGGGA AGCGAAGAGG ATTCCCCAGC
    ATCCTGGGAC CCCCACGGAG ACCAAGCCGT CATGACAACA GTGCAATTGG CAGAGCCATG
    GAACTACAGA AGGCGCGCCA CCCTAAGCAC TTATCCACAC CCTCATCTGT GAGTCCTGAA
    CCTCAGGACT CTGCCAAGTT GCGCCAGAGT GGGTTAGCAA ATGAAGGAAC AGACGCTGGA
    TACCTGCCTG CCAATTCCAT GTCTTCTGTA GCTTCAGGGG CCTCTTTTTC CCAGGAAGGA
    GGGAAAGAGA ATGATACAGG ATCAAAGCAA GTTGGAGAAA CATCAGCACC TGGAGACACC
    TTAGATGGCA CACCTCGTAC TCTCAATACT GTCTTTGATT TCCCACCACC TCCATTAGAC
    CAAGTGCAGG AGGAGGAATG TGAAGTAGAA AGGGTGACTG AACATGGGAC ACCAAAGCCC
    TTTCGAAAGT TTGACAGTGT AGCTTTTGGA GAAAGTCAAA GTGAGGATGA ACAATTTGAA
    AATGACTTAG AGACAGATCC ACCCAACTGG CAGCAGCTTG TTAGTCGAGA AGTGTTACTG
    GGACTAAAAC CTTGTGAAAT CAAAAGACAG GAAGTGATTA ATGAATTGTT CTACACTGAA
    AGAGCTCATG TTCGAACACT GAAGGTTCTT GATCAAGTGT TCTATCAGCG AGTATCCAGA
    GAAGGAATTC TGTCACCCTC AGAGCTACGG AAAATTTTTT CAAACTTGGA AGATATTCTT
    CAACTTCATA TTGGATTGAA TGAACAAATG AAGGCTGTTC GAAAGAGAAA TGAGACCTCT
    GTTATCGATC AGATTGGGGA AGATTTGCTG ACATGGTTCA GCGGACCAGG AGAGGAGAAA
    TTGAAACATG CTGCTGCTAC CTTTTGCAGT AACCAACCTT TCGCCCTGGA AATGATCAAA
    TCTCGTCAGA AAAAGGATTC TCGATTTCAG ACTTTTGTGC AAGATGCTGA AAGTAATCCA
    CTGTGTCGTC GTCTTCAACT GAAGGATATT ATTCCCACTC AAATGCAAAG GCTTACTAAG
    TACCCACTTC TGTTGGATAA TATTGCCAAA TACACAGAAT GGCCAACAGA AAGGGAGAAG
    GTGAAGAAAG CTGCAGATCA CTGTCGTCAG ATCTTAAATT ATGTAAATCA GGCTGTCAAG
    GAGGCAGAAA ACAAGCAGCG CCTAGAAGAT TATCAGCGTC GCCTTGATAC CTCCAGCCTG
    AAGTTGTCAG AGTACCCAAA TGTTGAAGAG CTCAGGAATT TGGATTTAAC AAAAAGGAAG
    ATGATTCATG AAGGGCCATT GGTTTGGAAG GTGAATAGAG ATAAAACTAT TGATTTATAC
    ACGTTGCTGC TGGAAGACAT TCTTGTATTG TTACAAAAGC AGGATGATAG ACTGGTTTTA
    AGGTGTCATA GTAAGATTCT GGCATCTACA GCTGATAGCA AACACACGTT TAGCCCTGTC
    ATTAAGTTGA GTACAGTGTT GGTTCGACAA GTGGCAACAG ATAACAAAGC TTTATTCGTC
    ATTTCCATGT CAGACAATGG CGCTCAGATT TATGAACTGG TGGCACAGAC AGTTTCTGAA
    AAGACTGTCT GGCAGGACCT AATCTGTCGG ATGGCTGCAT CAGTGAAGGA GCAATCCACA
    AAGCCAATTC CATTACCACA GTCAACACCT GGCGAAGGAG ATAATGATGA AGAAGATCCT
    TCAAAATTAA AAGAGGAGCA GCATGGCATT TCAGTCACTG GTTTGCAGAG TCCAGACAGA
    GATTTGGGAT TAGAATCTAC CTTAATATCG TCAAAACCTC AGTCTCATTC ACTGAGTACC
    TCTGGGAAAT CAGAGGTACG TGATCTGTTT GTGGCTGAGA GACAGTTTGC AAAGGAACAA
    CATACAGATG GGACACTAAA GGAAGTTGGA GAAGATTATC AAATCGCAAT CCCAGATTCA
    CACCTGCCTG TCTCAGAAGA ACGGTGGGCA TTGGATGCAC TAAGAAATTT GGGTTTGTTG
    AAGCAGTTGC TGGTGCAACA GCTAGGTTTG ACTGAGAAGA GCGTTCAGGA AGACTGGCAA
    CATTTCCCAA GATACAGAAC AGCCTCTCAG GGGCCGCAGA CAGACAGTGT CATCCAGAAC
    TCTGAAAATA TTAAGGCCTA TCATTCTGGT GAAGGACATA TGCCCTTTAG AACTGGAACT
    GGTGACATTG CAACTTGTTA CAGTCCACGG ACTTCAACTG AATCTTTTGC TCCACGGGAT
    TCAGTGGGAC TGGCACCCCA GGATAGCCAG GCAAGTAACA TTTTAGTAAT GGACCACATG
    ATTATGACCC CAGAGATGCC TACCATGGAG CCAGAAGGGG GTCTTGATGA CAGTGGAGAG
    CACTTTTTTG ATGCCCGTGA AGCACATAGT GATGAGAATC CATCAGAAGG TGATGGAGCA
    GTTAACAAGG AAGAGAAGGA TGTTAATTTA CGCATCTCAG GAAACTATTT GATCCTTGAT
    GGCTATGACC CAGTGCAGGA GAGTTCCACA GATGAGGAGG TTGCTTCCTC ACTTACCCTG
    CAGCCCATGA CAGGCATCCC TGCTGTGGAA TCCACCCACC AGCAGCAACA TTCTCCTCAG
    AATACTCACT CCGATGGGGC AATTTCACCA TTCACCCCCG AATTTCTGGT CCAGCAGCGC
    TGGGGAGCTA TGGAGTATTC CTGTTTTGAG ATCCAGAGTC CCTCCTCTTG TGCAGATTCA
    CAGAGCCAGA TCATGGAGTA CATTCATAAG ATAGAGGCTG ACCTTGAACA CTTAAAGAAG
    GTGGAGGAAA GTTACACCAT TCTTTGCCAA AGGCTGGCTG GATCAGCCCT CACAGACAAG
    CACTCAGATA AAAGTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu45709
    Clone ID Related Accession (Same CDS sequence) XM_017017421.1 , NM_001301084.1 , XM_011542720.2 , NM_001301084.2
    Accession Version NM_001301084.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4326bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-07-22
    Organism Homo sapiens(human)
    Product rho guanine nucleotide exchange factor 12 isoform 3
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DC350360.1, BX647785.1, AP000659.4, AF180681.1 and AP000758.5. Transcript Variant: This variant (3) uses an alternate first exon and initiates translation at a downstream AUG compared to variant 1. Thd resulting isoform (3) is shorter at the N-terminus compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BX647785.1, SRR1660805.161593.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1966682, SAMEA1968968 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    ATGCGGGCTG GAGTACAGAC AGGTGATCGA ATCATCAAGG TGAATGGAAC TCTGGTGACT 
    CATTCAAATC ATCTGGAGGT GGTGAAGCTA ATCAAATCTG GTTCCTATGT AGCTCTCACT
    GTTCAGGGAC GCCCACCTGG GTCGCCCCAG ATTCCACTTG CCGACTCTGA AGTAGAGCCG
    TCAGTCATTG GACATATGTC TCCCATCATG ACATCTCCTC ATTCACCTGG AGCATCTGGG
    AATATGGAGA GAATCACTAG TCCTGTGCTC ATGGGGGAGG AAAACAATGT GGTTCATAAC
    CAGAAAGTAG AAATTCTGAG AAAAATGTTA CAGAAAGAAC AGGAACGGCT ACAGTTATTG
    CAGGAAGATT ACAACCGAAC ACCTGCCCAA AGATTGCTAA AAGAGATCCA AGAGGCCAAG
    AAACACATTC CTCAGCTGCA AGAGCAGTTA TCCAAAGCCA CAGGCTCTGC TCAGGATGGA
    GCTGTAGTTA CACCCTCCAG ACCTTTAGGG GACACCCTAA CAGTCAGTGA GGCAGAAACA
    GATCCTGGAG ATGTACTGGG CAGGACTGAC TGTAGCAGTG GAGATGCTTC TCGGCCCAGT
    AGTGACAATG CAGATAGTCC CAAGAGTGGC CCAAAAGAGA GAATTTATCT AGAGGAAAAC
    CCAGAGAAAA GTGAAACAAT TCAGGACACT GACACTCAAT CACTTGTCGG AAGTCCCTCA
    ACCCGTATAG CACCTCATAT TATTGGAGCA GAAGATGATG ATTTTGGTAC TGAACATGAA
    CAGATCAATG GACAGTGCAG CTGTTTCCAG AGCATTGAAT TACTAAAATC TCGCCCGGCT
    CATTTGGCTG TTTTCTTACA CCATGTAGTT TCACAATTTG ACCCTGCGAC TTTGCTCTGT
    TATCTCTATT CAGACCTGTA TAAACATACC AATTCCAAAG AAACTCGTCG CATCTTCCTT
    GAGTTTCATC AGTTCTTTCT AGATCGATCA GCACACCTGA AAGTTTCTGT TCCTGATGAA
    ATGTCTGCAG ATCTAGAAAA GAGAAGACCT GAGCTCATTC CTGAGGATCT GCATCGCCAC
    TATATCCAAA CTATGCAAGA AAGAGTCCAT CCAGAAGTTC AAAGGCACTT AGAAGATTTT
    CGGCAGAAAC GTAGTATGGG ACTGACCTTG GCTGAAAGCG AGCTGACTAA ACTTGATGCA
    GAGCGAGACA AGGACCGATT GACTTTGGAG AAGGAGCGGA CATGTGCAGA ACAGATTGTT
    GCCAAAATTG AAGAAGTATT GATGACTGCT CAGGCTGTAG AGGAAGATAA GAGCTCCACC
    ATGCAGTATG TTATTCTCAT GTATATGAAG CATTTGGGAG TAAAAGTGAA AGAGCCTCGA
    AATTTGGAGC ACAAACGGGG TCGGATTGGA TTTCTTCCCA AAATCAAGCA AAGTATGAAG
    AAAGATAAAG AAGGGGAAGA AAAAGGGAAG CGAAGAGGAT TCCCCAGCAT CCTGGGACCC
    CCACGGAGAC CAAGCCGTCA TGACAACAGT GCAATTGGCA GAGCCATGGA ACTACAGAAG
    GCGCGCCACC CTAAGCACTT ATCCACACCC TCATCTGTGA GTCCTGAACC TCAGGACTCT
    GCCAAGTTGC GCCAGAGTGG GTTAGCAAAT GAAGGAACAG ACGCTGGATA CCTGCCTGCC
    AATTCCATGT CTTCTGTAGC TTCAGGGGCC TCTTTTTCCC AGGAAGGAGG GAAAGAGAAT
    GATACAGGAT CAAAGCAAGT TGGAGAAACA TCAGCACCTG GAGACACCTT AGATGGCACA
    CCTCGTACTC TCAATACTGT CTTTGATTTC CCACCACCTC CATTAGACCA AGTGCAGGAG
    GAGGAATGTG AAGTAGAAAG GGTGACTGAA CATGGGACAC CAAAGCCCTT TCGAAAGTTT
    GACAGTGTAG CTTTTGGAGA AAGTCAAAGT GAGGATGAAC AATTTGAAAA TGACTTAGAG
    ACAGATCCAC CCAACTGGCA GCAGCTTGTT AGTCGAGAAG TGTTACTGGG ACTAAAACCT
    TGTGAAATCA AAAGACAGGA AGTGATTAAT GAATTGTTCT ACACTGAAAG AGCTCATGTT
    CGAACACTGA AGGTTCTTGA TCAAGTGTTC TATCAGCGAG TATCCAGAGA AGGAATTCTG
    TCACCCTCAG AGCTACGGAA AATTTTTTCA AACTTGGAAG ATATTCTTCA ACTTCATATT
    GGATTGAATG AACAAATGAA GGCTGTTCGA AAGAGAAATG AGACCTCTGT TATCGATCAG
    ATTGGGGAAG ATTTGCTGAC ATGGTTCAGC GGACCAGGAG AGGAGAAATT GAAACATGCT
    GCTGCTACCT TTTGCAGTAA CCAACCTTTC GCCCTGGAAA TGATCAAATC TCGTCAGAAA
    AAGGATTCTC GATTTCAGAC TTTTGTGCAA GATGCTGAAA GTAATCCACT GTGTCGTCGT
    CTTCAACTGA AGGATATTAT TCCCACTCAA ATGCAAAGGC TTACTAAGTA CCCACTTCTG
    TTGGATAATA TTGCCAAATA CACAGAATGG CCAACAGAAA GGGAGAAGGT GAAGAAAGCT
    GCAGATCACT GTCGTCAGAT CTTAAATTAT GTAAATCAGG CTGTCAAGGA GGCAGAAAAC
    AAGCAGCGCC TAGAAGATTA TCAGCGTCGC CTTGATACCT CCAGCCTGAA GTTGTCAGAG
    TACCCAAATG TTGAAGAGCT CAGGAATTTG GATTTAACAA AAAGGAAGAT GATTCATGAA
    GGGCCATTGG TTTGGAAGGT GAATAGAGAT AAAACTATTG ATTTATACAC GTTGCTGCTG
    GAAGACATTC TTGTATTGTT ACAAAAGCAG GATGATAGAC TGGTTTTAAG GTGTCATAGT
    AAGATTCTGG CATCTACAGC TGATAGCAAA CACACGTTTA GCCCTGTCAT TAAGTTGAGT
    ACAGTGTTGG TTCGACAAGT GGCAACAGAT AACAAAGCTT TATTCGTCAT TTCCATGTCA
    GACAATGGCG CTCAGATTTA TGAACTGGTG GCACAGACAG TTTCTGAAAA GACTGTCTGG
    CAGGACCTAA TCTGTCGGAT GGCTGCATCA GTGAAGGAGC AATCCACAAA GCCAATTCCA
    TTACCACAGT CAACACCTGG CGAAGGAGAT AATGATGAAG AAGATCCTTC AAAATTAAAA
    GAGGAGCAGC ATGGCATTTC AGTCACTGGT TTGCAGAGTC CAGACAGAGA TTTGGGATTA
    GAATCTACCT TAATATCGTC AAAACCTCAG TCTCATTCAC TGAGTACCTC TGGGAAATCA
    GAGGTACGTG ATCTGTTTGT GGCTGAGAGA CAGTTTGCAA AGGAACAACA TACAGATGGG
    ACACTAAAGG AAGTTGGAGA AGATTATCAA ATCGCAATCC CAGATTCACA CCTGCCTGTC
    TCAGAAGAAC GGTGGGCATT GGATGCACTA AGAAATTTGG GTTTGTTGAA GCAGTTGCTG
    GTGCAACAGC TAGGTTTGAC TGAGAAGAGC GTTCAGGAAG ACTGGCAACA TTTCCCAAGA
    TACAGAACAG CCTCTCAGGG GCCGCAGACA GACAGTGTCA TCCAGAACTC TGAAAATATT
    AAGGCCTATC ATTCTGGTGA AGGACATATG CCCTTTAGAA CTGGAACTGG TGACATTGCA
    ACTTGTTACA GTCCACGGAC TTCAACTGAA TCTTTTGCTC CACGGGATTC AGTGGGACTG
    GCACCCCAGG ATAGCCAGGC AAGTAACATT TTAGTAATGG ACCACATGAT TATGACCCCA
    GAGATGCCTA CCATGGAGCC AGAAGGGGGT CTTGATGACA GTGGAGAGCA CTTTTTTGAT
    GCCCGTGAAG CACATAGTGA TGAGAATCCA TCAGAAGGTG ATGGAGCAGT TAACAAGGAA
    GAGAAGGATG TTAATTTACG CATCTCAGGA AACTATTTGA TCCTTGATGG CTATGACCCA
    GTGCAGGAGA GTTCCACAGA TGAGGAGGTT GCTTCCTCAC TTACCCTGCA GCCCATGACA
    GGCATCCCTG CTGTGGAATC CACCCACCAG CAGCAACATT CTCCTCAGAA TACTCACTCC
    GATGGGGCAA TTTCACCATT CACCCCCGAA TTTCTGGTCC AGCAGCGCTG GGGAGCTATG
    GAGTATTCCT GTTTTGAGAT CCAGAGTCCC TCCTCTTGTG CAGATTCACA GAGCCAGATC
    ATGGAGTACA TTCATAAGAT AGAGGCTGAC CTTGAACACT TAAAGAAGGT GGAGGAAAGT
    TACACCATTC TTTGCCAAAG GCTGGCTGGA TCAGCCCTCA CAGACAAGCA CTCAGATAAA
    AGTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001288013.1
    CDS471..4796
    Translation

    Target ORF information:

    RefSeq Version NM_001301084.1
    Organism Homo sapiens(human)
    Definition Homo sapiens Rho guanine nucleotide exchange factor 12 (ARHGEF12), transcript variant 3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001301084.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    ATGCGGGCTG GAGTACAGAC AGGTGATCGA ATCATCAAGG TGAATGGAAC TCTGGTGACT 
    CATTCAAATC ATCTGGAGGT GGTGAAGCTA ATCAAATCTG GTTCCTATGT AGCTCTCACT
    GTTCAGGGAC GCCCACCTGG GTCGCCCCAG ATTCCACTTG CCGACTCTGA AGTAGAGCCG
    TCAGTCATTG GACATATGTC TCCCATCATG ACATCTCCTC ATTCACCTGG AGCATCTGGG
    AATATGGAGA GAATCACTAG TCCTGTGCTC ATGGGGGAGG AAAACAATGT GGTTCATAAC
    CAGAAAGTAG AAATTCTGAG AAAAATGTTA CAGAAAGAAC AGGAACGGCT ACAGTTATTG
    CAGGAAGATT ACAACCGAAC ACCTGCCCAA AGATTGCTAA AAGAGATCCA AGAGGCCAAG
    AAACACATTC CTCAGCTGCA AGAGCAGTTA TCCAAAGCCA CAGGCTCTGC TCAGGATGGA
    GCTGTAGTTA CACCCTCCAG ACCTTTAGGG GACACCCTAA CAGTCAGTGA GGCAGAAACA
    GATCCTGGAG ATGTACTGGG CAGGACTGAC TGTAGCAGTG GAGATGCTTC TCGGCCCAGT
    AGTGACAATG CAGATAGTCC CAAGAGTGGC CCAAAAGAGA GAATTTATCT AGAGGAAAAC
    CCAGAGAAAA GTGAAACAAT TCAGGACACT GACACTCAAT CACTTGTCGG AAGTCCCTCA
    ACCCGTATAG CACCTCATAT TATTGGAGCA GAAGATGATG ATTTTGGTAC TGAACATGAA
    CAGATCAATG GACAGTGCAG CTGTTTCCAG AGCATTGAAT TACTAAAATC TCGCCCGGCT
    CATTTGGCTG TTTTCTTACA CCATGTAGTT TCACAATTTG ACCCTGCGAC TTTGCTCTGT
    TATCTCTATT CAGACCTGTA TAAACATACC AATTCCAAAG AAACTCGTCG CATCTTCCTT
    GAGTTTCATC AGTTCTTTCT AGATCGATCA GCACACCTGA AAGTTTCTGT TCCTGATGAA
    ATGTCTGCAG ATCTAGAAAA GAGAAGACCT GAGCTCATTC CTGAGGATCT GCATCGCCAC
    TATATCCAAA CTATGCAAGA AAGAGTCCAT CCAGAAGTTC AAAGGCACTT AGAAGATTTT
    CGGCAGAAAC GTAGTATGGG ACTGACCTTG GCTGAAAGCG AGCTGACTAA ACTTGATGCA
    GAGCGAGACA AGGACCGATT GACTTTGGAG AAGGAGCGGA CATGTGCAGA ACAGATTGTT
    GCCAAAATTG AAGAAGTATT GATGACTGCT CAGGCTGTAG AGGAAGATAA GAGCTCCACC
    ATGCAGTATG TTATTCTCAT GTATATGAAG CATTTGGGAG TAAAAGTGAA AGAGCCTCGA
    AATTTGGAGC ACAAACGGGG TCGGATTGGA TTTCTTCCCA AAATCAAGCA AAGTATGAAG
    AAAGATAAAG AAGGGGAAGA AAAAGGGAAG CGAAGAGGAT TCCCCAGCAT CCTGGGACCC
    CCACGGAGAC CAAGCCGTCA TGACAACAGT GCAATTGGCA GAGCCATGGA ACTACAGAAG
    GCGCGCCACC CTAAGCACTT ATCCACACCC TCATCTGTGA GTCCTGAACC TCAGGACTCT
    GCCAAGTTGC GCCAGAGTGG GTTAGCAAAT GAAGGAACAG ACGCTGGATA CCTGCCTGCC
    AATTCCATGT CTTCTGTAGC TTCAGGGGCC TCTTTTTCCC AGGAAGGAGG GAAAGAGAAT
    GATACAGGAT CAAAGCAAGT TGGAGAAACA TCAGCACCTG GAGACACCTT AGATGGCACA
    CCTCGTACTC TCAATACTGT CTTTGATTTC CCACCACCTC CATTAGACCA AGTGCAGGAG
    GAGGAATGTG AAGTAGAAAG GGTGACTGAA CATGGGACAC CAAAGCCCTT TCGAAAGTTT
    GACAGTGTAG CTTTTGGAGA AAGTCAAAGT GAGGATGAAC AATTTGAAAA TGACTTAGAG
    ACAGATCCAC CCAACTGGCA GCAGCTTGTT AGTCGAGAAG TGTTACTGGG ACTAAAACCT
    TGTGAAATCA AAAGACAGGA AGTGATTAAT GAATTGTTCT ACACTGAAAG AGCTCATGTT
    CGAACACTGA AGGTTCTTGA TCAAGTGTTC TATCAGCGAG TATCCAGAGA AGGAATTCTG
    TCACCCTCAG AGCTACGGAA AATTTTTTCA AACTTGGAAG ATATTCTTCA ACTTCATATT
    GGATTGAATG AACAAATGAA GGCTGTTCGA AAGAGAAATG AGACCTCTGT TATCGATCAG
    ATTGGGGAAG ATTTGCTGAC ATGGTTCAGC GGACCAGGAG AGGAGAAATT GAAACATGCT
    GCTGCTACCT TTTGCAGTAA CCAACCTTTC GCCCTGGAAA TGATCAAATC TCGTCAGAAA
    AAGGATTCTC GATTTCAGAC TTTTGTGCAA GATGCTGAAA GTAATCCACT GTGTCGTCGT
    CTTCAACTGA AGGATATTAT TCCCACTCAA ATGCAAAGGC TTACTAAGTA CCCACTTCTG
    TTGGATAATA TTGCCAAATA CACAGAATGG CCAACAGAAA GGGAGAAGGT GAAGAAAGCT
    GCAGATCACT GTCGTCAGAT CTTAAATTAT GTAAATCAGG CTGTCAAGGA GGCAGAAAAC
    AAGCAGCGCC TAGAAGATTA TCAGCGTCGC CTTGATACCT CCAGCCTGAA GTTGTCAGAG
    TACCCAAATG TTGAAGAGCT CAGGAATTTG GATTTAACAA AAAGGAAGAT GATTCATGAA
    GGGCCATTGG TTTGGAAGGT GAATAGAGAT AAAACTATTG ATTTATACAC GTTGCTGCTG
    GAAGACATTC TTGTATTGTT ACAAAAGCAG GATGATAGAC TGGTTTTAAG GTGTCATAGT
    AAGATTCTGG CATCTACAGC TGATAGCAAA CACACGTTTA GCCCTGTCAT TAAGTTGAGT
    ACAGTGTTGG TTCGACAAGT GGCAACAGAT AACAAAGCTT TATTCGTCAT TTCCATGTCA
    GACAATGGCG CTCAGATTTA TGAACTGGTG GCACAGACAG TTTCTGAAAA GACTGTCTGG
    CAGGACCTAA TCTGTCGGAT GGCTGCATCA GTGAAGGAGC AATCCACAAA GCCAATTCCA
    TTACCACAGT CAACACCTGG CGAAGGAGAT AATGATGAAG AAGATCCTTC AAAATTAAAA
    GAGGAGCAGC ATGGCATTTC AGTCACTGGT TTGCAGAGTC CAGACAGAGA TTTGGGATTA
    GAATCTACCT TAATATCGTC AAAACCTCAG TCTCATTCAC TGAGTACCTC TGGGAAATCA
    GAGGTACGTG ATCTGTTTGT GGCTGAGAGA CAGTTTGCAA AGGAACAACA TACAGATGGG
    ACACTAAAGG AAGTTGGAGA AGATTATCAA ATCGCAATCC CAGATTCACA CCTGCCTGTC
    TCAGAAGAAC GGTGGGCATT GGATGCACTA AGAAATTTGG GTTTGTTGAA GCAGTTGCTG
    GTGCAACAGC TAGGTTTGAC TGAGAAGAGC GTTCAGGAAG ACTGGCAACA TTTCCCAAGA
    TACAGAACAG CCTCTCAGGG GCCGCAGACA GACAGTGTCA TCCAGAACTC TGAAAATATT
    AAGGCCTATC ATTCTGGTGA AGGACATATG CCCTTTAGAA CTGGAACTGG TGACATTGCA
    ACTTGTTACA GTCCACGGAC TTCAACTGAA TCTTTTGCTC CACGGGATTC AGTGGGACTG
    GCACCCCAGG ATAGCCAGGC AAGTAACATT TTAGTAATGG ACCACATGAT TATGACCCCA
    GAGATGCCTA CCATGGAGCC AGAAGGGGGT CTTGATGACA GTGGAGAGCA CTTTTTTGAT
    GCCCGTGAAG CACATAGTGA TGAGAATCCA TCAGAAGGTG ATGGAGCAGT TAACAAGGAA
    GAGAAGGATG TTAATTTACG CATCTCAGGA AACTATTTGA TCCTTGATGG CTATGACCCA
    GTGCAGGAGA GTTCCACAGA TGAGGAGGTT GCTTCCTCAC TTACCCTGCA GCCCATGACA
    GGCATCCCTG CTGTGGAATC CACCCACCAG CAGCAACATT CTCCTCAGAA TACTCACTCC
    GATGGGGCAA TTTCACCATT CACCCCCGAA TTTCTGGTCC AGCAGCGCTG GGGAGCTATG
    GAGTATTCCT GTTTTGAGAT CCAGAGTCCC TCCTCTTGTG CAGATTCACA GAGCCAGATC
    ATGGAGTACA TTCATAAGAT AGAGGCTGAC CTTGAACACT TAAAGAAGGT GGAGGAAAGT
    TACACCATTC TTTGCCAAAG GCTGGCTGGA TCAGCCCTCA CAGACAAGCA CTCAGATAAA
    AGTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu45709
    Clone ID Related Accession (Same CDS sequence) XM_017017421.1 , NM_001301084.1 , XM_011542720.2 , NM_001301084.2
    Accession Version NM_001301084.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4326bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-10-24
    Organism Homo sapiens(human)
    Product rho guanine nucleotide exchange factor 12 isoform 3
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BX647785.1, AP000659.4, AF180681.1 and AP000758.5. On May 31, 2019 this sequence version replaced NM_001301084.1. Transcript Variant: This variant (3) uses an alternate first exon and initiates translation at a downstream AUG compared to variant 1. Thd resulting isoform (3) is shorter at the N-terminus compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660807.129029.1, SRR1660805.161593.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1966682, SAMEA1968968 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    ATGCGGGCTG GAGTACAGAC AGGTGATCGA ATCATCAAGG TGAATGGAAC TCTGGTGACT 
    CATTCAAATC ATCTGGAGGT GGTGAAGCTA ATCAAATCTG GTTCCTATGT AGCTCTCACT
    GTTCAGGGAC GCCCACCTGG GTCGCCCCAG ATTCCACTTG CCGACTCTGA AGTAGAGCCG
    TCAGTCATTG GACATATGTC TCCCATCATG ACATCTCCTC ATTCACCTGG AGCATCTGGG
    AATATGGAGA GAATCACTAG TCCTGTGCTC ATGGGGGAGG AAAACAATGT GGTTCATAAC
    CAGAAAGTAG AAATTCTGAG AAAAATGTTA CAGAAAGAAC AGGAACGGCT ACAGTTATTG
    CAGGAAGATT ACAACCGAAC ACCTGCCCAA AGATTGCTAA AAGAGATCCA AGAGGCCAAG
    AAACACATTC CTCAGCTGCA AGAGCAGTTA TCCAAAGCCA CAGGCTCTGC TCAGGATGGA
    GCTGTAGTTA CACCCTCCAG ACCTTTAGGG GACACCCTAA CAGTCAGTGA GGCAGAAACA
    GATCCTGGAG ATGTACTGGG CAGGACTGAC TGTAGCAGTG GAGATGCTTC TCGGCCCAGT
    AGTGACAATG CAGATAGTCC CAAGAGTGGC CCAAAAGAGA GAATTTATCT AGAGGAAAAC
    CCAGAGAAAA GTGAAACAAT TCAGGACACT GACACTCAAT CACTTGTCGG AAGTCCCTCA
    ACCCGTATAG CACCTCATAT TATTGGAGCA GAAGATGATG ATTTTGGTAC TGAACATGAA
    CAGATCAATG GACAGTGCAG CTGTTTCCAG AGCATTGAAT TACTAAAATC TCGCCCGGCT
    CATTTGGCTG TTTTCTTACA CCATGTAGTT TCACAATTTG ACCCTGCGAC TTTGCTCTGT
    TATCTCTATT CAGACCTGTA TAAACATACC AATTCCAAAG AAACTCGTCG CATCTTCCTT
    GAGTTTCATC AGTTCTTTCT AGATCGATCA GCACACCTGA AAGTTTCTGT TCCTGATGAA
    ATGTCTGCAG ATCTAGAAAA GAGAAGACCT GAGCTCATTC CTGAGGATCT GCATCGCCAC
    TATATCCAAA CTATGCAAGA AAGAGTCCAT CCAGAAGTTC AAAGGCACTT AGAAGATTTT
    CGGCAGAAAC GTAGTATGGG ACTGACCTTG GCTGAAAGCG AGCTGACTAA ACTTGATGCA
    GAGCGAGACA AGGACCGATT GACTTTGGAG AAGGAGCGGA CATGTGCAGA ACAGATTGTT
    GCCAAAATTG AAGAAGTATT GATGACTGCT CAGGCTGTAG AGGAAGATAA GAGCTCCACC
    ATGCAGTATG TTATTCTCAT GTATATGAAG CATTTGGGAG TAAAAGTGAA AGAGCCTCGA
    AATTTGGAGC ACAAACGGGG TCGGATTGGA TTTCTTCCCA AAATCAAGCA AAGTATGAAG
    AAAGATAAAG AAGGGGAAGA AAAAGGGAAG CGAAGAGGAT TCCCCAGCAT CCTGGGACCC
    CCACGGAGAC CAAGCCGTCA TGACAACAGT GCAATTGGCA GAGCCATGGA ACTACAGAAG
    GCGCGCCACC CTAAGCACTT ATCCACACCC TCATCTGTGA GTCCTGAACC TCAGGACTCT
    GCCAAGTTGC GCCAGAGTGG GTTAGCAAAT GAAGGAACAG ACGCTGGATA CCTGCCTGCC
    AATTCCATGT CTTCTGTAGC TTCAGGGGCC TCTTTTTCCC AGGAAGGAGG GAAAGAGAAT
    GATACAGGAT CAAAGCAAGT TGGAGAAACA TCAGCACCTG GAGACACCTT AGATGGCACA
    CCTCGTACTC TCAATACTGT CTTTGATTTC CCACCACCTC CATTAGACCA AGTGCAGGAG
    GAGGAATGTG AAGTAGAAAG GGTGACTGAA CATGGGACAC CAAAGCCCTT TCGAAAGTTT
    GACAGTGTAG CTTTTGGAGA AAGTCAAAGT GAGGATGAAC AATTTGAAAA TGACTTAGAG
    ACAGATCCAC CCAACTGGCA GCAGCTTGTT AGTCGAGAAG TGTTACTGGG ACTAAAACCT
    TGTGAAATCA AAAGACAGGA AGTGATTAAT GAATTGTTCT ACACTGAAAG AGCTCATGTT
    CGAACACTGA AGGTTCTTGA TCAAGTGTTC TATCAGCGAG TATCCAGAGA AGGAATTCTG
    TCACCCTCAG AGCTACGGAA AATTTTTTCA AACTTGGAAG ATATTCTTCA ACTTCATATT
    GGATTGAATG AACAAATGAA GGCTGTTCGA AAGAGAAATG AGACCTCTGT TATCGATCAG
    ATTGGGGAAG ATTTGCTGAC ATGGTTCAGC GGACCAGGAG AGGAGAAATT GAAACATGCT
    GCTGCTACCT TTTGCAGTAA CCAACCTTTC GCCCTGGAAA TGATCAAATC TCGTCAGAAA
    AAGGATTCTC GATTTCAGAC TTTTGTGCAA GATGCTGAAA GTAATCCACT GTGTCGTCGT
    CTTCAACTGA AGGATATTAT TCCCACTCAA ATGCAAAGGC TTACTAAGTA CCCACTTCTG
    TTGGATAATA TTGCCAAATA CACAGAATGG CCAACAGAAA GGGAGAAGGT GAAGAAAGCT
    GCAGATCACT GTCGTCAGAT CTTAAATTAT GTAAATCAGG CTGTCAAGGA GGCAGAAAAC
    AAGCAGCGCC TAGAAGATTA TCAGCGTCGC CTTGATACCT CCAGCCTGAA GTTGTCAGAG
    TACCCAAATG TTGAAGAGCT CAGGAATTTG GATTTAACAA AAAGGAAGAT GATTCATGAA
    GGGCCATTGG TTTGGAAGGT GAATAGAGAT AAAACTATTG ATTTATACAC GTTGCTGCTG
    GAAGACATTC TTGTATTGTT ACAAAAGCAG GATGATAGAC TGGTTTTAAG GTGTCATAGT
    AAGATTCTGG CATCTACAGC TGATAGCAAA CACACGTTTA GCCCTGTCAT TAAGTTGAGT
    ACAGTGTTGG TTCGACAAGT GGCAACAGAT AACAAAGCTT TATTCGTCAT TTCCATGTCA
    GACAATGGCG CTCAGATTTA TGAACTGGTG GCACAGACAG TTTCTGAAAA GACTGTCTGG
    CAGGACCTAA TCTGTCGGAT GGCTGCATCA GTGAAGGAGC AATCCACAAA GCCAATTCCA
    TTACCACAGT CAACACCTGG CGAAGGAGAT AATGATGAAG AAGATCCTTC AAAATTAAAA
    GAGGAGCAGC ATGGCATTTC AGTCACTGGT TTGCAGAGTC CAGACAGAGA TTTGGGATTA
    GAATCTACCT TAATATCGTC AAAACCTCAG TCTCATTCAC TGAGTACCTC TGGGAAATCA
    GAGGTACGTG ATCTGTTTGT GGCTGAGAGA CAGTTTGCAA AGGAACAACA TACAGATGGG
    ACACTAAAGG AAGTTGGAGA AGATTATCAA ATCGCAATCC CAGATTCACA CCTGCCTGTC
    TCAGAAGAAC GGTGGGCATT GGATGCACTA AGAAATTTGG GTTTGTTGAA GCAGTTGCTG
    GTGCAACAGC TAGGTTTGAC TGAGAAGAGC GTTCAGGAAG ACTGGCAACA TTTCCCAAGA
    TACAGAACAG CCTCTCAGGG GCCGCAGACA GACAGTGTCA TCCAGAACTC TGAAAATATT
    AAGGCCTATC ATTCTGGTGA AGGACATATG CCCTTTAGAA CTGGAACTGG TGACATTGCA
    ACTTGTTACA GTCCACGGAC TTCAACTGAA TCTTTTGCTC CACGGGATTC AGTGGGACTG
    GCACCCCAGG ATAGCCAGGC AAGTAACATT TTAGTAATGG ACCACATGAT TATGACCCCA
    GAGATGCCTA CCATGGAGCC AGAAGGGGGT CTTGATGACA GTGGAGAGCA CTTTTTTGAT
    GCCCGTGAAG CACATAGTGA TGAGAATCCA TCAGAAGGTG ATGGAGCAGT TAACAAGGAA
    GAGAAGGATG TTAATTTACG CATCTCAGGA AACTATTTGA TCCTTGATGG CTATGACCCA
    GTGCAGGAGA GTTCCACAGA TGAGGAGGTT GCTTCCTCAC TTACCCTGCA GCCCATGACA
    GGCATCCCTG CTGTGGAATC CACCCACCAG CAGCAACATT CTCCTCAGAA TACTCACTCC
    GATGGGGCAA TTTCACCATT CACCCCCGAA TTTCTGGTCC AGCAGCGCTG GGGAGCTATG
    GAGTATTCCT GTTTTGAGAT CCAGAGTCCC TCCTCTTGTG CAGATTCACA GAGCCAGATC
    ATGGAGTACA TTCATAAGAT AGAGGCTGAC CTTGAACACT TAAAGAAGGT GGAGGAAAGT
    TACACCATTC TTTGCCAAAG GCTGGCTGGA TCAGCCCTCA CAGACAAGCA CTCAGATAAA
    AGTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001288013.1
    CDS438..4763
    Translation

    Target ORF information:

    RefSeq Version NM_001301084.2
    Organism Homo sapiens(human)
    Definition Homo sapiens Rho guanine nucleotide exchange factor 12 (ARHGEF12), transcript variant 3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001301084.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    ATGCGGGCTG GAGTACAGAC AGGTGATCGA ATCATCAAGG TGAATGGAAC TCTGGTGACT 
    CATTCAAATC ATCTGGAGGT GGTGAAGCTA ATCAAATCTG GTTCCTATGT AGCTCTCACT
    GTTCAGGGAC GCCCACCTGG GTCGCCCCAG ATTCCACTTG CCGACTCTGA AGTAGAGCCG
    TCAGTCATTG GACATATGTC TCCCATCATG ACATCTCCTC ATTCACCTGG AGCATCTGGG
    AATATGGAGA GAATCACTAG TCCTGTGCTC ATGGGGGAGG AAAACAATGT GGTTCATAAC
    CAGAAAGTAG AAATTCTGAG AAAAATGTTA CAGAAAGAAC AGGAACGGCT ACAGTTATTG
    CAGGAAGATT ACAACCGAAC ACCTGCCCAA AGATTGCTAA AAGAGATCCA AGAGGCCAAG
    AAACACATTC CTCAGCTGCA AGAGCAGTTA TCCAAAGCCA CAGGCTCTGC TCAGGATGGA
    GCTGTAGTTA CACCCTCCAG ACCTTTAGGG GACACCCTAA CAGTCAGTGA GGCAGAAACA
    GATCCTGGAG ATGTACTGGG CAGGACTGAC TGTAGCAGTG GAGATGCTTC TCGGCCCAGT
    AGTGACAATG CAGATAGTCC CAAGAGTGGC CCAAAAGAGA GAATTTATCT AGAGGAAAAC
    CCAGAGAAAA GTGAAACAAT TCAGGACACT GACACTCAAT CACTTGTCGG AAGTCCCTCA
    ACCCGTATAG CACCTCATAT TATTGGAGCA GAAGATGATG ATTTTGGTAC TGAACATGAA
    CAGATCAATG GACAGTGCAG CTGTTTCCAG AGCATTGAAT TACTAAAATC TCGCCCGGCT
    CATTTGGCTG TTTTCTTACA CCATGTAGTT TCACAATTTG ACCCTGCGAC TTTGCTCTGT
    TATCTCTATT CAGACCTGTA TAAACATACC AATTCCAAAG AAACTCGTCG CATCTTCCTT
    GAGTTTCATC AGTTCTTTCT AGATCGATCA GCACACCTGA AAGTTTCTGT TCCTGATGAA
    ATGTCTGCAG ATCTAGAAAA GAGAAGACCT GAGCTCATTC CTGAGGATCT GCATCGCCAC
    TATATCCAAA CTATGCAAGA AAGAGTCCAT CCAGAAGTTC AAAGGCACTT AGAAGATTTT
    CGGCAGAAAC GTAGTATGGG ACTGACCTTG GCTGAAAGCG AGCTGACTAA ACTTGATGCA
    GAGCGAGACA AGGACCGATT GACTTTGGAG AAGGAGCGGA CATGTGCAGA ACAGATTGTT
    GCCAAAATTG AAGAAGTATT GATGACTGCT CAGGCTGTAG AGGAAGATAA GAGCTCCACC
    ATGCAGTATG TTATTCTCAT GTATATGAAG CATTTGGGAG TAAAAGTGAA AGAGCCTCGA
    AATTTGGAGC ACAAACGGGG TCGGATTGGA TTTCTTCCCA AAATCAAGCA AAGTATGAAG
    AAAGATAAAG AAGGGGAAGA AAAAGGGAAG CGAAGAGGAT TCCCCAGCAT CCTGGGACCC
    CCACGGAGAC CAAGCCGTCA TGACAACAGT GCAATTGGCA GAGCCATGGA ACTACAGAAG
    GCGCGCCACC CTAAGCACTT ATCCACACCC TCATCTGTGA GTCCTGAACC TCAGGACTCT
    GCCAAGTTGC GCCAGAGTGG GTTAGCAAAT GAAGGAACAG ACGCTGGATA CCTGCCTGCC
    AATTCCATGT CTTCTGTAGC TTCAGGGGCC TCTTTTTCCC AGGAAGGAGG GAAAGAGAAT
    GATACAGGAT CAAAGCAAGT TGGAGAAACA TCAGCACCTG GAGACACCTT AGATGGCACA
    CCTCGTACTC TCAATACTGT CTTTGATTTC CCACCACCTC CATTAGACCA AGTGCAGGAG
    GAGGAATGTG AAGTAGAAAG GGTGACTGAA CATGGGACAC CAAAGCCCTT TCGAAAGTTT
    GACAGTGTAG CTTTTGGAGA AAGTCAAAGT GAGGATGAAC AATTTGAAAA TGACTTAGAG
    ACAGATCCAC CCAACTGGCA GCAGCTTGTT AGTCGAGAAG TGTTACTGGG ACTAAAACCT
    TGTGAAATCA AAAGACAGGA AGTGATTAAT GAATTGTTCT ACACTGAAAG AGCTCATGTT
    CGAACACTGA AGGTTCTTGA TCAAGTGTTC TATCAGCGAG TATCCAGAGA AGGAATTCTG
    TCACCCTCAG AGCTACGGAA AATTTTTTCA AACTTGGAAG ATATTCTTCA ACTTCATATT
    GGATTGAATG AACAAATGAA GGCTGTTCGA AAGAGAAATG AGACCTCTGT TATCGATCAG
    ATTGGGGAAG ATTTGCTGAC ATGGTTCAGC GGACCAGGAG AGGAGAAATT GAAACATGCT
    GCTGCTACCT TTTGCAGTAA CCAACCTTTC GCCCTGGAAA TGATCAAATC TCGTCAGAAA
    AAGGATTCTC GATTTCAGAC TTTTGTGCAA GATGCTGAAA GTAATCCACT GTGTCGTCGT
    CTTCAACTGA AGGATATTAT TCCCACTCAA ATGCAAAGGC TTACTAAGTA CCCACTTCTG
    TTGGATAATA TTGCCAAATA CACAGAATGG CCAACAGAAA GGGAGAAGGT GAAGAAAGCT
    GCAGATCACT GTCGTCAGAT CTTAAATTAT GTAAATCAGG CTGTCAAGGA GGCAGAAAAC
    AAGCAGCGCC TAGAAGATTA TCAGCGTCGC CTTGATACCT CCAGCCTGAA GTTGTCAGAG
    TACCCAAATG TTGAAGAGCT CAGGAATTTG GATTTAACAA AAAGGAAGAT GATTCATGAA
    GGGCCATTGG TTTGGAAGGT GAATAGAGAT AAAACTATTG ATTTATACAC GTTGCTGCTG
    GAAGACATTC TTGTATTGTT ACAAAAGCAG GATGATAGAC TGGTTTTAAG GTGTCATAGT
    AAGATTCTGG CATCTACAGC TGATAGCAAA CACACGTTTA GCCCTGTCAT TAAGTTGAGT
    ACAGTGTTGG TTCGACAAGT GGCAACAGAT AACAAAGCTT TATTCGTCAT TTCCATGTCA
    GACAATGGCG CTCAGATTTA TGAACTGGTG GCACAGACAG TTTCTGAAAA GACTGTCTGG
    CAGGACCTAA TCTGTCGGAT GGCTGCATCA GTGAAGGAGC AATCCACAAA GCCAATTCCA
    TTACCACAGT CAACACCTGG CGAAGGAGAT AATGATGAAG AAGATCCTTC AAAATTAAAA
    GAGGAGCAGC ATGGCATTTC AGTCACTGGT TTGCAGAGTC CAGACAGAGA TTTGGGATTA
    GAATCTACCT TAATATCGTC AAAACCTCAG TCTCATTCAC TGAGTACCTC TGGGAAATCA
    GAGGTACGTG ATCTGTTTGT GGCTGAGAGA CAGTTTGCAA AGGAACAACA TACAGATGGG
    ACACTAAAGG AAGTTGGAGA AGATTATCAA ATCGCAATCC CAGATTCACA CCTGCCTGTC
    TCAGAAGAAC GGTGGGCATT GGATGCACTA AGAAATTTGG GTTTGTTGAA GCAGTTGCTG
    GTGCAACAGC TAGGTTTGAC TGAGAAGAGC GTTCAGGAAG ACTGGCAACA TTTCCCAAGA
    TACAGAACAG CCTCTCAGGG GCCGCAGACA GACAGTGTCA TCCAGAACTC TGAAAATATT
    AAGGCCTATC ATTCTGGTGA AGGACATATG CCCTTTAGAA CTGGAACTGG TGACATTGCA
    ACTTGTTACA GTCCACGGAC TTCAACTGAA TCTTTTGCTC CACGGGATTC AGTGGGACTG
    GCACCCCAGG ATAGCCAGGC AAGTAACATT TTAGTAATGG ACCACATGAT TATGACCCCA
    GAGATGCCTA CCATGGAGCC AGAAGGGGGT CTTGATGACA GTGGAGAGCA CTTTTTTGAT
    GCCCGTGAAG CACATAGTGA TGAGAATCCA TCAGAAGGTG ATGGAGCAGT TAACAAGGAA
    GAGAAGGATG TTAATTTACG CATCTCAGGA AACTATTTGA TCCTTGATGG CTATGACCCA
    GTGCAGGAGA GTTCCACAGA TGAGGAGGTT GCTTCCTCAC TTACCCTGCA GCCCATGACA
    GGCATCCCTG CTGTGGAATC CACCCACCAG CAGCAACATT CTCCTCAGAA TACTCACTCC
    GATGGGGCAA TTTCACCATT CACCCCCGAA TTTCTGGTCC AGCAGCGCTG GGGAGCTATG
    GAGTATTCCT GTTTTGAGAT CCAGAGTCCC TCCTCTTGTG CAGATTCACA GAGCCAGATC
    ATGGAGTACA TTCATAAGAT AGAGGCTGAC CTTGAACACT TAAAGAAGGT GGAGGAAAGT
    TACACCATTC TTTGCCAAAG GCTGGCTGGA TCAGCCCTCA CAGACAAGCA CTCAGATAAA
    AGTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu45707
    Clone ID Related Accession (Same CDS sequence) XM_006718805.3
    Accession Version XM_006718805.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4560bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product rho guanine nucleotide exchange factor 12 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000011.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_006718805.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    ATGAGTGGCA CACAGTCTAC TATCACCGAC AGGTTTCCCC TCAAAAAACC TATAAGGCAT 
    GGAAGTATTT TGAACCGAGA GTCACCAACA GATAAGAAGC AGAAAGTTGA GCGCATTGCA
    TCACATGATT TTGACCCCAC AGATAGCTCC TCCAAGAAGA CAAAGTCTAG TTCAGAGGAG
    AGTAGATCCG AGATATATGG TCTTGTTCAG CGTTGCGTAA TCATCCAGAA AGATGACAAT
    GGATTTGGGC TGACGGTCAG TGGAGACAAT CCAGTCTTCG TACAGTCTGT CAAAGAAGAT
    GGAGCAGCCA TGCGGGCTGG AGTACAGACA GGTGATCGAA TCATCAAGGT GAATGGAACT
    CTGGTGACTC ATTCAAATCA TCTGGAGGTG GTGAAGCTAA TCAAATCTGG TTCCTATGTA
    GCTCTCACTG TTCAGGGACG CCCACCTGGG TCGCCCCAGA TTCCACTTGC CGACTCTGAA
    GTAGAGCCGT CAGTCATTGG ACATATGTCT CCCATCATGA CATCTCCTCA TTCACCTGGA
    GCATCTGGGA ATATGGAGAG AATCACTAGT CCTGTGCTCA TGGGGGAGGA AAACAATGTG
    GTTCATAACC AGAAAGTAGA AATTCTGAGA AAAATGTTAC AGAAAGAACA GGAACGGCTA
    CAGTTATTGC AGGAAGATTA CAACCGAACA CCTGCCCAAA GATTGCTAAA AGAGATCCAA
    GAGGCCAAGA AACACATTCC TCAGCTGCAA GAGCAGTTAT CCAAAGCCAC AGGCTCTGCT
    CAGGATGGAG CTGTAGTTAC ACCCTCCAGA CCTTTAGGGG ACACCCTAAC AGTCAGTGAG
    GCAGAAACAG ATCCTGGAGA TGTACTGGGC AGGACTGACT GTAGCAGTGG AGATGCTTCT
    CGGCCCAGTA GTGACAATGC AGATGACACT CAATCACTTG TCGGAAGTCC CTCAACCCGT
    ATAGCACCTC ATATTATTGG AGCAGAAGAT GATGATTTTG GTACTGAACA TGAACAGATC
    AATGGACAGT GCAGCTGTTT CCAGAGCATT GAATTACTAA AATCTCGCCC GGCTCATTTG
    GCTGTTTTCT TACACCATGT AGTTTCACAA TTTGACCCTG CGACTTTGCT CTGTTATCTC
    TATTCAGACC TGTATAAACA TACCAATTCC AAAGAAACTC GTCGCATCTT CCTTGAGTTT
    CATCAGTTCT TTCTAGATCG ATCAGCACAC CTGAAAGTTT CTGTTCCTGA TGAAATGTCT
    GCAGATCTAG AAAAGAGAAG ACCTGAGCTC ATTCCTGAGG ATCTGCATCG CCACTATATC
    CAAACTATGC AAGAAAGAGT CCATCCAGAA GTTCAAAGGC ACTTAGAAGA TTTTCGGCAG
    AAACGTAGTA TGGGACTGAC CTTGGCTGAA AGCGAGCTGA CTAAACTTGA TGCAGAGCGA
    GACAAGGACC GATTGACTTT GGAGAAGGAG CGGACATGTG CAGAACAGAT TGTTGCCAAA
    ATTGAAGAAG TATTGATGAC TGCTCAGGCT GTAGAGGAAG ATAAGAGCTC CACCATGCAG
    TATGTTATTC TCATGTATAT GAAGCATTTG GGAGTAAAAG TGAAAGAGCC TCGAAATTTG
    GAGCACAAAC GGGGTCGGAT TGGATTTCTT CCCAAAATCA AGCAAAGTAT GAAGAAAGAT
    AAAGAAGGGG AAGAAAAAGG GAAGCGAAGA GGATTCCCCA GCATCCTGGG ACCCCCACGG
    AGACCAAGCC GTCATGACAA CAGTGCAATT GGCAGAGCCA TGGAACTACA GAAGGCGCGC
    CACCCTAAGC ACTTATCCAC ACCCTCATCT GTGAGTCCTG AACCTCAGGA CTCTGCCAAG
    TTGCGCCAGA GTGGGTTAGC AAATGAAGGA ACAGACGCTG GATACCTGCC TGCCAATTCC
    ATGTCTTCTG TAGCTTCAGG GGCCTCTTTT TCCCAGGAAG GAGGGAAAGA GAATGATACA
    GGATCAAAGC AAGTTGGAGA AACATCAGCA CCTGGAGACA CCTTAGATGG CACACCTCGT
    ACTCTCAATA CTGTCTTTGA TTTCCCACCA CCTCCATTAG ACCAAGTGCA GGAGGAGGAA
    TGTGAAGTAG AAAGGGTGAC TGAACATGGG ACACCAAAGC CCTTTCGAAA GTTTGACAGT
    GTAGCTTTTG GAGAAAGTCA AAGTGAGGAT GAACAATTTG AAAATGACTT AGAGACAGAT
    CCACCCAACT GGCAGCAGCT TGTTAGTCGA GAAGTGTTAC TGGGACTAAA ACCTTGTGAA
    ATCAAAAGAC AGGAAGTGAT TAATGAATTG TTCTACACTG AAAGAGCTCA TGTTCGAACA
    CTGAAGGTTC TTGATCAAGT GTTCTATCAG CGAGTATCCA GAGAAGGAAT TCTGTCACCC
    TCAGAGCTAC GGAAAATTTT TTCAAACTTG GAAGATATTC TTCAACTTCA TATTGGATTG
    AATGAACAAA TGAAGGCTGT TCGAAAGAGA AATGAGACCT CTGTTATCGA TCAGATTGGG
    GAAGATTTGC TGACATGGTT CAGCGGACCA GGAGAGGAGA AATTGAAACA TGCTGCTGCT
    ACCTTTTGCA GTAACCAACC TTTCGCCCTG GAAATGATCA AATCTCGTCA GAAAAAGGAT
    TCTCGATTTC AGACTTTTGT GCAAGATGCT GAAAGTAATC CACTGTGTCG TCGTCTTCAA
    CTGAAGGATA TTATTCCCAC TCAAATGCAA AGGCTTACTA AGTACCCACT TCTGTTGGAT
    AATATTGCCA AATACACAGA ATGGCCAACA GAAAGGGAGA AGGTGAAGAA AGCTGCAGAT
    CACTGTCGTC AGATCTTAAA TTATGTAAAT CAGGCTGTCA AGGAGGCAGA AAACAAGCAG
    CGCCTAGAAG ATTATCAGCG TCGCCTTGAT ACCTCCAGCC TGAAGTTGTC AGAGTACCCA
    AATGTTGAAG AGCTCAGGAA TTTGGATTTA ACAAAAAGGA AGATGATTCA TGAAGGGCCA
    TTGGTTTGGA AGGTGAATAG AGATAAAACT ATTGATTTAT ACACGTTGCT GCTGGAAGAC
    ATTCTTGTAT TGTTACAAAA GCAGGATGAT AGACTGGTTT TAAGGTGTCA TAGTAAGATT
    CTGGCATCTA CAGCTGATAG CAAACACACG TTTAGCCCTG TCATTAAGTT GAGTACAGTG
    TTGGTTCGAC AAGTGGCAAC AGATAACAAA GCTTTATTCG TCATTTCCAT GTCAGACAAT
    GGCGCTCAGA TTTATGAACT GGTGGCACAG ACAGTTTCTG AAAAGACTGT CTGGCAGGAC
    CTAATCTGTC GGATGGCTGC ATCAGTGAAG GAGCAATCCA CAAAGCCAAT TCCATTACCA
    CAGTCAACAC CTGGCGAAGG AGATAATGAT GAAGAAGATC CTTCAAAATT AAAAGAGGAG
    CAGCATGGCA TTTCAGTCAC TGGTTTGCAG AGTCCAGACA GAGATTTGGG ATTAGAATCT
    ACCTTAATAT CGTCAAAACC TCAGTCTCAT TCACTGAGTA CCTCTGGGAA ATCAGAGGTA
    CGTGATCTGT TTGTGGCTGA GAGACAGTTT GCAAAGGAAC AACATACAGA TGGGACACTA
    AAGGAAGTTG GAGAAGATTA TCAAATCGCA ATCCCAGATT CACACCTGCC TGTCTCAGAA
    GAACGGTGGG CATTGGATGC ACTAAGAAAT TTGGGTTTGT TGAAGCAGTT GCTGGTGCAA
    CAGCTAGGTT TGACTGAGAA GAGCGTTCAG GAAGACTGGC AACATTTCCC AAGATACAGA
    ACAGCCTCTC AGGGGCCGCA GACAGACAGT GTCATCCAGA ACTCTGAAAA TATTAAGGCC
    TATCATTCTG GTGAAGGACA TATGCCCTTT AGAACTGGAA CTGGTGACAT TGCAACTTGT
    TACAGTCCAC GGACTTCAAC TGAATCTTTT GCTCCACGGG ATTCAGTGGG ACTGGCACCC
    CAGGATAGCC AGGCAAGTAA CATTTTAGTA ATGGACCACA TGATTATGAC CCCAGAGATG
    CCTACCATGG AGCCAGAAGG GGGTCTTGAT GACAGTGGAG AGCACTTTTT TGATGCCCGT
    GAAGCACATA GTGATGAGAA TCCATCAGAA GGTGATGGAG CAGTTAACAA GGAAGAGAAG
    GATGTTAATT TACGCATCTC AGGAAACTAT TTGATCCTTG ATGGCTATGA CCCAGTGCAG
    GAGAGTTCCA CAGATGAGGA GGTTGCTTCC TCACTTACCC TGCAGCCCAT GACAGGCATC
    CCTGCTGTGG AATCCACCCA CCAGCAGCAA CATTCTCCTC AGAATACTCA CTCCGATGGG
    GCAATTTCAC CATTCACCCC CGAATTTCTG GTCCAGCAGC GCTGGGGAGC TATGGAGTAT
    TCCTGTTTTG AGATCCAGAG TCCCTCCTCT TGTGCAGATT CACAGAGCCA GATCATGGAG
    TACATTCATA AGATAGAGGC TGACCTTGAA CACTTAAAGA AGGTGGAGGA AAGTTACACC
    ATTCTTTGCC AAAGGCTGGC TGGATCAGCC CTCACAGACA AGCACTCAGA TAAAAGTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006718868.1
    CDS695..5254
    Translation

    Target ORF information:

    RefSeq Version XM_006718805.3
    Organism Homo sapiens(human)
    Definition Homo sapiens Rho guanine nucleotide exchange factor 12 (ARHGEF12), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006718805.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    ATGAGTGGCA CACAGTCTAC TATCACCGAC AGGTTTCCCC TCAAAAAACC TATAAGGCAT 
    GGAAGTATTT TGAACCGAGA GTCACCAACA GATAAGAAGC AGAAAGTTGA GCGCATTGCA
    TCACATGATT TTGACCCCAC AGATAGCTCC TCCAAGAAGA CAAAGTCTAG TTCAGAGGAG
    AGTAGATCCG AGATATATGG TCTTGTTCAG CGTTGCGTAA TCATCCAGAA AGATGACAAT
    GGATTTGGGC TGACGGTCAG TGGAGACAAT CCAGTCTTCG TACAGTCTGT CAAAGAAGAT
    GGAGCAGCCA TGCGGGCTGG AGTACAGACA GGTGATCGAA TCATCAAGGT GAATGGAACT
    CTGGTGACTC ATTCAAATCA TCTGGAGGTG GTGAAGCTAA TCAAATCTGG TTCCTATGTA
    GCTCTCACTG TTCAGGGACG CCCACCTGGG TCGCCCCAGA TTCCACTTGC CGACTCTGAA
    GTAGAGCCGT CAGTCATTGG ACATATGTCT CCCATCATGA CATCTCCTCA TTCACCTGGA
    GCATCTGGGA ATATGGAGAG AATCACTAGT CCTGTGCTCA TGGGGGAGGA AAACAATGTG
    GTTCATAACC AGAAAGTAGA AATTCTGAGA AAAATGTTAC AGAAAGAACA GGAACGGCTA
    CAGTTATTGC AGGAAGATTA CAACCGAACA CCTGCCCAAA GATTGCTAAA AGAGATCCAA
    GAGGCCAAGA AACACATTCC TCAGCTGCAA GAGCAGTTAT CCAAAGCCAC AGGCTCTGCT
    CAGGATGGAG CTGTAGTTAC ACCCTCCAGA CCTTTAGGGG ACACCCTAAC AGTCAGTGAG
    GCAGAAACAG ATCCTGGAGA TGTACTGGGC AGGACTGACT GTAGCAGTGG AGATGCTTCT
    CGGCCCAGTA GTGACAATGC AGATGACACT CAATCACTTG TCGGAAGTCC CTCAACCCGT
    ATAGCACCTC ATATTATTGG AGCAGAAGAT GATGATTTTG GTACTGAACA TGAACAGATC
    AATGGACAGT GCAGCTGTTT CCAGAGCATT GAATTACTAA AATCTCGCCC GGCTCATTTG
    GCTGTTTTCT TACACCATGT AGTTTCACAA TTTGACCCTG CGACTTTGCT CTGTTATCTC
    TATTCAGACC TGTATAAACA TACCAATTCC AAAGAAACTC GTCGCATCTT CCTTGAGTTT
    CATCAGTTCT TTCTAGATCG ATCAGCACAC CTGAAAGTTT CTGTTCCTGA TGAAATGTCT
    GCAGATCTAG AAAAGAGAAG ACCTGAGCTC ATTCCTGAGG ATCTGCATCG CCACTATATC
    CAAACTATGC AAGAAAGAGT CCATCCAGAA GTTCAAAGGC ACTTAGAAGA TTTTCGGCAG
    AAACGTAGTA TGGGACTGAC CTTGGCTGAA AGCGAGCTGA CTAAACTTGA TGCAGAGCGA
    GACAAGGACC GATTGACTTT GGAGAAGGAG CGGACATGTG CAGAACAGAT TGTTGCCAAA
    ATTGAAGAAG TATTGATGAC TGCTCAGGCT GTAGAGGAAG ATAAGAGCTC CACCATGCAG
    TATGTTATTC TCATGTATAT GAAGCATTTG GGAGTAAAAG TGAAAGAGCC TCGAAATTTG
    GAGCACAAAC GGGGTCGGAT TGGATTTCTT CCCAAAATCA AGCAAAGTAT GAAGAAAGAT
    AAAGAAGGGG AAGAAAAAGG GAAGCGAAGA GGATTCCCCA GCATCCTGGG ACCCCCACGG
    AGACCAAGCC GTCATGACAA CAGTGCAATT GGCAGAGCCA TGGAACTACA GAAGGCGCGC
    CACCCTAAGC ACTTATCCAC ACCCTCATCT GTGAGTCCTG AACCTCAGGA CTCTGCCAAG
    TTGCGCCAGA GTGGGTTAGC AAATGAAGGA ACAGACGCTG GATACCTGCC TGCCAATTCC
    ATGTCTTCTG TAGCTTCAGG GGCCTCTTTT TCCCAGGAAG GAGGGAAAGA GAATGATACA
    GGATCAAAGC AAGTTGGAGA AACATCAGCA CCTGGAGACA CCTTAGATGG CACACCTCGT
    ACTCTCAATA CTGTCTTTGA TTTCCCACCA CCTCCATTAG ACCAAGTGCA GGAGGAGGAA
    TGTGAAGTAG AAAGGGTGAC TGAACATGGG ACACCAAAGC CCTTTCGAAA GTTTGACAGT
    GTAGCTTTTG GAGAAAGTCA AAGTGAGGAT GAACAATTTG AAAATGACTT AGAGACAGAT
    CCACCCAACT GGCAGCAGCT TGTTAGTCGA GAAGTGTTAC TGGGACTAAA ACCTTGTGAA
    ATCAAAAGAC AGGAAGTGAT TAATGAATTG TTCTACACTG AAAGAGCTCA TGTTCGAACA
    CTGAAGGTTC TTGATCAAGT GTTCTATCAG CGAGTATCCA GAGAAGGAAT TCTGTCACCC
    TCAGAGCTAC GGAAAATTTT TTCAAACTTG GAAGATATTC TTCAACTTCA TATTGGATTG
    AATGAACAAA TGAAGGCTGT TCGAAAGAGA AATGAGACCT CTGTTATCGA TCAGATTGGG
    GAAGATTTGC TGACATGGTT CAGCGGACCA GGAGAGGAGA AATTGAAACA TGCTGCTGCT
    ACCTTTTGCA GTAACCAACC TTTCGCCCTG GAAATGATCA AATCTCGTCA GAAAAAGGAT
    TCTCGATTTC AGACTTTTGT GCAAGATGCT GAAAGTAATC CACTGTGTCG TCGTCTTCAA
    CTGAAGGATA TTATTCCCAC TCAAATGCAA AGGCTTACTA AGTACCCACT TCTGTTGGAT
    AATATTGCCA AATACACAGA ATGGCCAACA GAAAGGGAGA AGGTGAAGAA AGCTGCAGAT
    CACTGTCGTC AGATCTTAAA TTATGTAAAT CAGGCTGTCA AGGAGGCAGA AAACAAGCAG
    CGCCTAGAAG ATTATCAGCG TCGCCTTGAT ACCTCCAGCC TGAAGTTGTC AGAGTACCCA
    AATGTTGAAG AGCTCAGGAA TTTGGATTTA ACAAAAAGGA AGATGATTCA TGAAGGGCCA
    TTGGTTTGGA AGGTGAATAG AGATAAAACT ATTGATTTAT ACACGTTGCT GCTGGAAGAC
    ATTCTTGTAT TGTTACAAAA GCAGGATGAT AGACTGGTTT TAAGGTGTCA TAGTAAGATT
    CTGGCATCTA CAGCTGATAG CAAACACACG TTTAGCCCTG TCATTAAGTT GAGTACAGTG
    TTGGTTCGAC AAGTGGCAAC AGATAACAAA GCTTTATTCG TCATTTCCAT GTCAGACAAT
    GGCGCTCAGA TTTATGAACT GGTGGCACAG ACAGTTTCTG AAAAGACTGT CTGGCAGGAC
    CTAATCTGTC GGATGGCTGC ATCAGTGAAG GAGCAATCCA CAAAGCCAAT TCCATTACCA
    CAGTCAACAC CTGGCGAAGG AGATAATGAT GAAGAAGATC CTTCAAAATT AAAAGAGGAG
    CAGCATGGCA TTTCAGTCAC TGGTTTGCAG AGTCCAGACA GAGATTTGGG ATTAGAATCT
    ACCTTAATAT CGTCAAAACC TCAGTCTCAT TCACTGAGTA CCTCTGGGAA ATCAGAGGTA
    CGTGATCTGT TTGTGGCTGA GAGACAGTTT GCAAAGGAAC AACATACAGA TGGGACACTA
    AAGGAAGTTG GAGAAGATTA TCAAATCGCA ATCCCAGATT CACACCTGCC TGTCTCAGAA
    GAACGGTGGG CATTGGATGC ACTAAGAAAT TTGGGTTTGT TGAAGCAGTT GCTGGTGCAA
    CAGCTAGGTT TGACTGAGAA GAGCGTTCAG GAAGACTGGC AACATTTCCC AAGATACAGA
    ACAGCCTCTC AGGGGCCGCA GACAGACAGT GTCATCCAGA ACTCTGAAAA TATTAAGGCC
    TATCATTCTG GTGAAGGACA TATGCCCTTT AGAACTGGAA CTGGTGACAT TGCAACTTGT
    TACAGTCCAC GGACTTCAAC TGAATCTTTT GCTCCACGGG ATTCAGTGGG ACTGGCACCC
    CAGGATAGCC AGGCAAGTAA CATTTTAGTA ATGGACCACA TGATTATGAC CCCAGAGATG
    CCTACCATGG AGCCAGAAGG GGGTCTTGAT GACAGTGGAG AGCACTTTTT TGATGCCCGT
    GAAGCACATA GTGATGAGAA TCCATCAGAA GGTGATGGAG CAGTTAACAA GGAAGAGAAG
    GATGTTAATT TACGCATCTC AGGAAACTAT TTGATCCTTG ATGGCTATGA CCCAGTGCAG
    GAGAGTTCCA CAGATGAGGA GGTTGCTTCC TCACTTACCC TGCAGCCCAT GACAGGCATC
    CCTGCTGTGG AATCCACCCA CCAGCAGCAA CATTCTCCTC AGAATACTCA CTCCGATGGG
    GCAATTTCAC CATTCACCCC CGAATTTCTG GTCCAGCAGC GCTGGGGAGC TATGGAGTAT
    TCCTGTTTTG AGATCCAGAG TCCCTCCTCT TGTGCAGATT CACAGAGCCA GATCATGGAG
    TACATTCATA AGATAGAGGC TGACCTTGAA CACTTAAAGA AGGTGGAGGA AAGTTACACC
    ATTCTTTGCC AAAGGCTGGC TGGATCAGCC CTCACAGACA AGCACTCAGA TAAAAGTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu45709
    Clone ID Related Accession (Same CDS sequence) XM_017017421.1 , NM_001301084.1 , XM_011542720.2 , NM_001301084.2
    Accession Version XM_017017421.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4326bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product rho guanine nucleotide exchange factor 12 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000011.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    ATGCGGGCTG GAGTACAGAC AGGTGATCGA ATCATCAAGG TGAATGGAAC TCTGGTGACT 
    CATTCAAATC ATCTGGAGGT GGTGAAGCTA ATCAAATCTG GTTCCTATGT AGCTCTCACT
    GTTCAGGGAC GCCCACCTGG GTCGCCCCAG ATTCCACTTG CCGACTCTGA AGTAGAGCCG
    TCAGTCATTG GACATATGTC TCCCATCATG ACATCTCCTC ATTCACCTGG AGCATCTGGG
    AATATGGAGA GAATCACTAG TCCTGTGCTC ATGGGGGAGG AAAACAATGT GGTTCATAAC
    CAGAAAGTAG AAATTCTGAG AAAAATGTTA CAGAAAGAAC AGGAACGGCT ACAGTTATTG
    CAGGAAGATT ACAACCGAAC ACCTGCCCAA AGATTGCTAA AAGAGATCCA AGAGGCCAAG
    AAACACATTC CTCAGCTGCA AGAGCAGTTA TCCAAAGCCA CAGGCTCTGC TCAGGATGGA
    GCTGTAGTTA CACCCTCCAG ACCTTTAGGG GACACCCTAA CAGTCAGTGA GGCAGAAACA
    GATCCTGGAG ATGTACTGGG CAGGACTGAC TGTAGCAGTG GAGATGCTTC TCGGCCCAGT
    AGTGACAATG CAGATAGTCC CAAGAGTGGC CCAAAAGAGA GAATTTATCT AGAGGAAAAC
    CCAGAGAAAA GTGAAACAAT TCAGGACACT GACACTCAAT CACTTGTCGG AAGTCCCTCA
    ACCCGTATAG CACCTCATAT TATTGGAGCA GAAGATGATG ATTTTGGTAC TGAACATGAA
    CAGATCAATG GACAGTGCAG CTGTTTCCAG AGCATTGAAT TACTAAAATC TCGCCCGGCT
    CATTTGGCTG TTTTCTTACA CCATGTAGTT TCACAATTTG ACCCTGCGAC TTTGCTCTGT
    TATCTCTATT CAGACCTGTA TAAACATACC AATTCCAAAG AAACTCGTCG CATCTTCCTT
    GAGTTTCATC AGTTCTTTCT AGATCGATCA GCACACCTGA AAGTTTCTGT TCCTGATGAA
    ATGTCTGCAG ATCTAGAAAA GAGAAGACCT GAGCTCATTC CTGAGGATCT GCATCGCCAC
    TATATCCAAA CTATGCAAGA AAGAGTCCAT CCAGAAGTTC AAAGGCACTT AGAAGATTTT
    CGGCAGAAAC GTAGTATGGG ACTGACCTTG GCTGAAAGCG AGCTGACTAA ACTTGATGCA
    GAGCGAGACA AGGACCGATT GACTTTGGAG AAGGAGCGGA CATGTGCAGA ACAGATTGTT
    GCCAAAATTG AAGAAGTATT GATGACTGCT CAGGCTGTAG AGGAAGATAA GAGCTCCACC
    ATGCAGTATG TTATTCTCAT GTATATGAAG CATTTGGGAG TAAAAGTGAA AGAGCCTCGA
    AATTTGGAGC ACAAACGGGG TCGGATTGGA TTTCTTCCCA AAATCAAGCA AAGTATGAAG
    AAAGATAAAG AAGGGGAAGA AAAAGGGAAG CGAAGAGGAT TCCCCAGCAT CCTGGGACCC
    CCACGGAGAC CAAGCCGTCA TGACAACAGT GCAATTGGCA GAGCCATGGA ACTACAGAAG
    GCGCGCCACC CTAAGCACTT ATCCACACCC TCATCTGTGA GTCCTGAACC TCAGGACTCT
    GCCAAGTTGC GCCAGAGTGG GTTAGCAAAT GAAGGAACAG ACGCTGGATA CCTGCCTGCC
    AATTCCATGT CTTCTGTAGC TTCAGGGGCC TCTTTTTCCC AGGAAGGAGG GAAAGAGAAT
    GATACAGGAT CAAAGCAAGT TGGAGAAACA TCAGCACCTG GAGACACCTT AGATGGCACA
    CCTCGTACTC TCAATACTGT CTTTGATTTC CCACCACCTC CATTAGACCA AGTGCAGGAG
    GAGGAATGTG AAGTAGAAAG GGTGACTGAA CATGGGACAC CAAAGCCCTT TCGAAAGTTT
    GACAGTGTAG CTTTTGGAGA AAGTCAAAGT GAGGATGAAC AATTTGAAAA TGACTTAGAG
    ACAGATCCAC CCAACTGGCA GCAGCTTGTT AGTCGAGAAG TGTTACTGGG ACTAAAACCT
    TGTGAAATCA AAAGACAGGA AGTGATTAAT GAATTGTTCT ACACTGAAAG AGCTCATGTT
    CGAACACTGA AGGTTCTTGA TCAAGTGTTC TATCAGCGAG TATCCAGAGA AGGAATTCTG
    TCACCCTCAG AGCTACGGAA AATTTTTTCA AACTTGGAAG ATATTCTTCA ACTTCATATT
    GGATTGAATG AACAAATGAA GGCTGTTCGA AAGAGAAATG AGACCTCTGT TATCGATCAG
    ATTGGGGAAG ATTTGCTGAC ATGGTTCAGC GGACCAGGAG AGGAGAAATT GAAACATGCT
    GCTGCTACCT TTTGCAGTAA CCAACCTTTC GCCCTGGAAA TGATCAAATC TCGTCAGAAA
    AAGGATTCTC GATTTCAGAC TTTTGTGCAA GATGCTGAAA GTAATCCACT GTGTCGTCGT
    CTTCAACTGA AGGATATTAT TCCCACTCAA ATGCAAAGGC TTACTAAGTA CCCACTTCTG
    TTGGATAATA TTGCCAAATA CACAGAATGG CCAACAGAAA GGGAGAAGGT GAAGAAAGCT
    GCAGATCACT GTCGTCAGAT CTTAAATTAT GTAAATCAGG CTGTCAAGGA GGCAGAAAAC
    AAGCAGCGCC TAGAAGATTA TCAGCGTCGC CTTGATACCT CCAGCCTGAA GTTGTCAGAG
    TACCCAAATG TTGAAGAGCT CAGGAATTTG GATTTAACAA AAAGGAAGAT GATTCATGAA
    GGGCCATTGG TTTGGAAGGT GAATAGAGAT AAAACTATTG ATTTATACAC GTTGCTGCTG
    GAAGACATTC TTGTATTGTT ACAAAAGCAG GATGATAGAC TGGTTTTAAG GTGTCATAGT
    AAGATTCTGG CATCTACAGC TGATAGCAAA CACACGTTTA GCCCTGTCAT TAAGTTGAGT
    ACAGTGTTGG TTCGACAAGT GGCAACAGAT AACAAAGCTT TATTCGTCAT TTCCATGTCA
    GACAATGGCG CTCAGATTTA TGAACTGGTG GCACAGACAG TTTCTGAAAA GACTGTCTGG
    CAGGACCTAA TCTGTCGGAT GGCTGCATCA GTGAAGGAGC AATCCACAAA GCCAATTCCA
    TTACCACAGT CAACACCTGG CGAAGGAGAT AATGATGAAG AAGATCCTTC AAAATTAAAA
    GAGGAGCAGC ATGGCATTTC AGTCACTGGT TTGCAGAGTC CAGACAGAGA TTTGGGATTA
    GAATCTACCT TAATATCGTC AAAACCTCAG TCTCATTCAC TGAGTACCTC TGGGAAATCA
    GAGGTACGTG ATCTGTTTGT GGCTGAGAGA CAGTTTGCAA AGGAACAACA TACAGATGGG
    ACACTAAAGG AAGTTGGAGA AGATTATCAA ATCGCAATCC CAGATTCACA CCTGCCTGTC
    TCAGAAGAAC GGTGGGCATT GGATGCACTA AGAAATTTGG GTTTGTTGAA GCAGTTGCTG
    GTGCAACAGC TAGGTTTGAC TGAGAAGAGC GTTCAGGAAG ACTGGCAACA TTTCCCAAGA
    TACAGAACAG CCTCTCAGGG GCCGCAGACA GACAGTGTCA TCCAGAACTC TGAAAATATT
    AAGGCCTATC ATTCTGGTGA AGGACATATG CCCTTTAGAA CTGGAACTGG TGACATTGCA
    ACTTGTTACA GTCCACGGAC TTCAACTGAA TCTTTTGCTC CACGGGATTC AGTGGGACTG
    GCACCCCAGG ATAGCCAGGC AAGTAACATT TTAGTAATGG ACCACATGAT TATGACCCCA
    GAGATGCCTA CCATGGAGCC AGAAGGGGGT CTTGATGACA GTGGAGAGCA CTTTTTTGAT
    GCCCGTGAAG CACATAGTGA TGAGAATCCA TCAGAAGGTG ATGGAGCAGT TAACAAGGAA
    GAGAAGGATG TTAATTTACG CATCTCAGGA AACTATTTGA TCCTTGATGG CTATGACCCA
    GTGCAGGAGA GTTCCACAGA TGAGGAGGTT GCTTCCTCAC TTACCCTGCA GCCCATGACA
    GGCATCCCTG CTGTGGAATC CACCCACCAG CAGCAACATT CTCCTCAGAA TACTCACTCC
    GATGGGGCAA TTTCACCATT CACCCCCGAA TTTCTGGTCC AGCAGCGCTG GGGAGCTATG
    GAGTATTCCT GTTTTGAGAT CCAGAGTCCC TCCTCTTGTG CAGATTCACA GAGCCAGATC
    ATGGAGTACA TTCATAAGAT AGAGGCTGAC CTTGAACACT TAAAGAAGGT GGAGGAAAGT
    TACACCATTC TTTGCCAAAG GCTGGCTGGA TCAGCCCTCA CAGACAAGCA CTCAGATAAA
    AGTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_016872910.1
    CDS861..5186
    Translation

    Target ORF information:

    RefSeq Version XM_017017421.1
    Organism Homo sapiens(human)
    Definition Homo sapiens Rho guanine nucleotide exchange factor 12 (ARHGEF12), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017017421.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    ATGCGGGCTG GAGTACAGAC AGGTGATCGA ATCATCAAGG TGAATGGAAC TCTGGTGACT 
    CATTCAAATC ATCTGGAGGT GGTGAAGCTA ATCAAATCTG GTTCCTATGT AGCTCTCACT
    GTTCAGGGAC GCCCACCTGG GTCGCCCCAG ATTCCACTTG CCGACTCTGA AGTAGAGCCG
    TCAGTCATTG GACATATGTC TCCCATCATG ACATCTCCTC ATTCACCTGG AGCATCTGGG
    AATATGGAGA GAATCACTAG TCCTGTGCTC ATGGGGGAGG AAAACAATGT GGTTCATAAC
    CAGAAAGTAG AAATTCTGAG AAAAATGTTA CAGAAAGAAC AGGAACGGCT ACAGTTATTG
    CAGGAAGATT ACAACCGAAC ACCTGCCCAA AGATTGCTAA AAGAGATCCA AGAGGCCAAG
    AAACACATTC CTCAGCTGCA AGAGCAGTTA TCCAAAGCCA CAGGCTCTGC TCAGGATGGA
    GCTGTAGTTA CACCCTCCAG ACCTTTAGGG GACACCCTAA CAGTCAGTGA GGCAGAAACA
    GATCCTGGAG ATGTACTGGG CAGGACTGAC TGTAGCAGTG GAGATGCTTC TCGGCCCAGT
    AGTGACAATG CAGATAGTCC CAAGAGTGGC CCAAAAGAGA GAATTTATCT AGAGGAAAAC
    CCAGAGAAAA GTGAAACAAT TCAGGACACT GACACTCAAT CACTTGTCGG AAGTCCCTCA
    ACCCGTATAG CACCTCATAT TATTGGAGCA GAAGATGATG ATTTTGGTAC TGAACATGAA
    CAGATCAATG GACAGTGCAG CTGTTTCCAG AGCATTGAAT TACTAAAATC TCGCCCGGCT
    CATTTGGCTG TTTTCTTACA CCATGTAGTT TCACAATTTG ACCCTGCGAC TTTGCTCTGT
    TATCTCTATT CAGACCTGTA TAAACATACC AATTCCAAAG AAACTCGTCG CATCTTCCTT
    GAGTTTCATC AGTTCTTTCT AGATCGATCA GCACACCTGA AAGTTTCTGT TCCTGATGAA
    ATGTCTGCAG ATCTAGAAAA GAGAAGACCT GAGCTCATTC CTGAGGATCT GCATCGCCAC
    TATATCCAAA CTATGCAAGA AAGAGTCCAT CCAGAAGTTC AAAGGCACTT AGAAGATTTT
    CGGCAGAAAC GTAGTATGGG ACTGACCTTG GCTGAAAGCG AGCTGACTAA ACTTGATGCA
    GAGCGAGACA AGGACCGATT GACTTTGGAG AAGGAGCGGA CATGTGCAGA ACAGATTGTT
    GCCAAAATTG AAGAAGTATT GATGACTGCT CAGGCTGTAG AGGAAGATAA GAGCTCCACC
    ATGCAGTATG TTATTCTCAT GTATATGAAG CATTTGGGAG TAAAAGTGAA AGAGCCTCGA
    AATTTGGAGC ACAAACGGGG TCGGATTGGA TTTCTTCCCA AAATCAAGCA AAGTATGAAG
    AAAGATAAAG AAGGGGAAGA AAAAGGGAAG CGAAGAGGAT TCCCCAGCAT CCTGGGACCC
    CCACGGAGAC CAAGCCGTCA TGACAACAGT GCAATTGGCA GAGCCATGGA ACTACAGAAG
    GCGCGCCACC CTAAGCACTT ATCCACACCC TCATCTGTGA GTCCTGAACC TCAGGACTCT
    GCCAAGTTGC GCCAGAGTGG GTTAGCAAAT GAAGGAACAG ACGCTGGATA CCTGCCTGCC
    AATTCCATGT CTTCTGTAGC TTCAGGGGCC TCTTTTTCCC AGGAAGGAGG GAAAGAGAAT
    GATACAGGAT CAAAGCAAGT TGGAGAAACA TCAGCACCTG GAGACACCTT AGATGGCACA
    CCTCGTACTC TCAATACTGT CTTTGATTTC CCACCACCTC CATTAGACCA AGTGCAGGAG
    GAGGAATGTG AAGTAGAAAG GGTGACTGAA CATGGGACAC CAAAGCCCTT TCGAAAGTTT
    GACAGTGTAG CTTTTGGAGA AAGTCAAAGT GAGGATGAAC AATTTGAAAA TGACTTAGAG
    ACAGATCCAC CCAACTGGCA GCAGCTTGTT AGTCGAGAAG TGTTACTGGG ACTAAAACCT
    TGTGAAATCA AAAGACAGGA AGTGATTAAT GAATTGTTCT ACACTGAAAG AGCTCATGTT
    CGAACACTGA AGGTTCTTGA TCAAGTGTTC TATCAGCGAG TATCCAGAGA AGGAATTCTG
    TCACCCTCAG AGCTACGGAA AATTTTTTCA AACTTGGAAG ATATTCTTCA ACTTCATATT
    GGATTGAATG AACAAATGAA GGCTGTTCGA AAGAGAAATG AGACCTCTGT TATCGATCAG
    ATTGGGGAAG ATTTGCTGAC ATGGTTCAGC GGACCAGGAG AGGAGAAATT GAAACATGCT
    GCTGCTACCT TTTGCAGTAA CCAACCTTTC GCCCTGGAAA TGATCAAATC TCGTCAGAAA
    AAGGATTCTC GATTTCAGAC TTTTGTGCAA GATGCTGAAA GTAATCCACT GTGTCGTCGT
    CTTCAACTGA AGGATATTAT TCCCACTCAA ATGCAAAGGC TTACTAAGTA CCCACTTCTG
    TTGGATAATA TTGCCAAATA CACAGAATGG CCAACAGAAA GGGAGAAGGT GAAGAAAGCT
    GCAGATCACT GTCGTCAGAT CTTAAATTAT GTAAATCAGG CTGTCAAGGA GGCAGAAAAC
    AAGCAGCGCC TAGAAGATTA TCAGCGTCGC CTTGATACCT CCAGCCTGAA GTTGTCAGAG
    TACCCAAATG TTGAAGAGCT CAGGAATTTG GATTTAACAA AAAGGAAGAT GATTCATGAA
    GGGCCATTGG TTTGGAAGGT GAATAGAGAT AAAACTATTG ATTTATACAC GTTGCTGCTG
    GAAGACATTC TTGTATTGTT ACAAAAGCAG GATGATAGAC TGGTTTTAAG GTGTCATAGT
    AAGATTCTGG CATCTACAGC TGATAGCAAA CACACGTTTA GCCCTGTCAT TAAGTTGAGT
    ACAGTGTTGG TTCGACAAGT GGCAACAGAT AACAAAGCTT TATTCGTCAT TTCCATGTCA
    GACAATGGCG CTCAGATTTA TGAACTGGTG GCACAGACAG TTTCTGAAAA GACTGTCTGG
    CAGGACCTAA TCTGTCGGAT GGCTGCATCA GTGAAGGAGC AATCCACAAA GCCAATTCCA
    TTACCACAGT CAACACCTGG CGAAGGAGAT AATGATGAAG AAGATCCTTC AAAATTAAAA
    GAGGAGCAGC ATGGCATTTC AGTCACTGGT TTGCAGAGTC CAGACAGAGA TTTGGGATTA
    GAATCTACCT TAATATCGTC AAAACCTCAG TCTCATTCAC TGAGTACCTC TGGGAAATCA
    GAGGTACGTG ATCTGTTTGT GGCTGAGAGA CAGTTTGCAA AGGAACAACA TACAGATGGG
    ACACTAAAGG AAGTTGGAGA AGATTATCAA ATCGCAATCC CAGATTCACA CCTGCCTGTC
    TCAGAAGAAC GGTGGGCATT GGATGCACTA AGAAATTTGG GTTTGTTGAA GCAGTTGCTG
    GTGCAACAGC TAGGTTTGAC TGAGAAGAGC GTTCAGGAAG ACTGGCAACA TTTCCCAAGA
    TACAGAACAG CCTCTCAGGG GCCGCAGACA GACAGTGTCA TCCAGAACTC TGAAAATATT
    AAGGCCTATC ATTCTGGTGA AGGACATATG CCCTTTAGAA CTGGAACTGG TGACATTGCA
    ACTTGTTACA GTCCACGGAC TTCAACTGAA TCTTTTGCTC CACGGGATTC AGTGGGACTG
    GCACCCCAGG ATAGCCAGGC AAGTAACATT TTAGTAATGG ACCACATGAT TATGACCCCA
    GAGATGCCTA CCATGGAGCC AGAAGGGGGT CTTGATGACA GTGGAGAGCA CTTTTTTGAT
    GCCCGTGAAG CACATAGTGA TGAGAATCCA TCAGAAGGTG ATGGAGCAGT TAACAAGGAA
    GAGAAGGATG TTAATTTACG CATCTCAGGA AACTATTTGA TCCTTGATGG CTATGACCCA
    GTGCAGGAGA GTTCCACAGA TGAGGAGGTT GCTTCCTCAC TTACCCTGCA GCCCATGACA
    GGCATCCCTG CTGTGGAATC CACCCACCAG CAGCAACATT CTCCTCAGAA TACTCACTCC
    GATGGGGCAA TTTCACCATT CACCCCCGAA TTTCTGGTCC AGCAGCGCTG GGGAGCTATG
    GAGTATTCCT GTTTTGAGAT CCAGAGTCCC TCCTCTTGTG CAGATTCACA GAGCCAGATC
    ATGGAGTACA TTCATAAGAT AGAGGCTGAC CTTGAACACT TAAAGAAGGT GGAGGAAAGT
    TACACCATTC TTTGCCAAAG GCTGGCTGGA TCAGCCCTCA CAGACAAGCA CTCAGATAAA
    AGTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu45709
    Clone ID Related Accession (Same CDS sequence) XM_017017421.1 , NM_001301084.1 , XM_011542720.2 , NM_001301084.2
    Accession Version XM_011542720.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4326bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product rho guanine nucleotide exchange factor 12 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000011.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011542720.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    ATGCGGGCTG GAGTACAGAC AGGTGATCGA ATCATCAAGG TGAATGGAAC TCTGGTGACT 
    CATTCAAATC ATCTGGAGGT GGTGAAGCTA ATCAAATCTG GTTCCTATGT AGCTCTCACT
    GTTCAGGGAC GCCCACCTGG GTCGCCCCAG ATTCCACTTG CCGACTCTGA AGTAGAGCCG
    TCAGTCATTG GACATATGTC TCCCATCATG ACATCTCCTC ATTCACCTGG AGCATCTGGG
    AATATGGAGA GAATCACTAG TCCTGTGCTC ATGGGGGAGG AAAACAATGT GGTTCATAAC
    CAGAAAGTAG AAATTCTGAG AAAAATGTTA CAGAAAGAAC AGGAACGGCT ACAGTTATTG
    CAGGAAGATT ACAACCGAAC ACCTGCCCAA AGATTGCTAA AAGAGATCCA AGAGGCCAAG
    AAACACATTC CTCAGCTGCA AGAGCAGTTA TCCAAAGCCA CAGGCTCTGC TCAGGATGGA
    GCTGTAGTTA CACCCTCCAG ACCTTTAGGG GACACCCTAA CAGTCAGTGA GGCAGAAACA
    GATCCTGGAG ATGTACTGGG CAGGACTGAC TGTAGCAGTG GAGATGCTTC TCGGCCCAGT
    AGTGACAATG CAGATAGTCC CAAGAGTGGC CCAAAAGAGA GAATTTATCT AGAGGAAAAC
    CCAGAGAAAA GTGAAACAAT TCAGGACACT GACACTCAAT CACTTGTCGG AAGTCCCTCA
    ACCCGTATAG CACCTCATAT TATTGGAGCA GAAGATGATG ATTTTGGTAC TGAACATGAA
    CAGATCAATG GACAGTGCAG CTGTTTCCAG AGCATTGAAT TACTAAAATC TCGCCCGGCT
    CATTTGGCTG TTTTCTTACA CCATGTAGTT TCACAATTTG ACCCTGCGAC TTTGCTCTGT
    TATCTCTATT CAGACCTGTA TAAACATACC AATTCCAAAG AAACTCGTCG CATCTTCCTT
    GAGTTTCATC AGTTCTTTCT AGATCGATCA GCACACCTGA AAGTTTCTGT TCCTGATGAA
    ATGTCTGCAG ATCTAGAAAA GAGAAGACCT GAGCTCATTC CTGAGGATCT GCATCGCCAC
    TATATCCAAA CTATGCAAGA AAGAGTCCAT CCAGAAGTTC AAAGGCACTT AGAAGATTTT
    CGGCAGAAAC GTAGTATGGG ACTGACCTTG GCTGAAAGCG AGCTGACTAA ACTTGATGCA
    GAGCGAGACA AGGACCGATT GACTTTGGAG AAGGAGCGGA CATGTGCAGA ACAGATTGTT
    GCCAAAATTG AAGAAGTATT GATGACTGCT CAGGCTGTAG AGGAAGATAA GAGCTCCACC
    ATGCAGTATG TTATTCTCAT GTATATGAAG CATTTGGGAG TAAAAGTGAA AGAGCCTCGA
    AATTTGGAGC ACAAACGGGG TCGGATTGGA TTTCTTCCCA AAATCAAGCA AAGTATGAAG
    AAAGATAAAG AAGGGGAAGA AAAAGGGAAG CGAAGAGGAT TCCCCAGCAT CCTGGGACCC
    CCACGGAGAC CAAGCCGTCA TGACAACAGT GCAATTGGCA GAGCCATGGA ACTACAGAAG
    GCGCGCCACC CTAAGCACTT ATCCACACCC TCATCTGTGA GTCCTGAACC TCAGGACTCT
    GCCAAGTTGC GCCAGAGTGG GTTAGCAAAT GAAGGAACAG ACGCTGGATA CCTGCCTGCC
    AATTCCATGT CTTCTGTAGC TTCAGGGGCC TCTTTTTCCC AGGAAGGAGG GAAAGAGAAT
    GATACAGGAT CAAAGCAAGT TGGAGAAACA TCAGCACCTG GAGACACCTT AGATGGCACA
    CCTCGTACTC TCAATACTGT CTTTGATTTC CCACCACCTC CATTAGACCA AGTGCAGGAG
    GAGGAATGTG AAGTAGAAAG GGTGACTGAA CATGGGACAC CAAAGCCCTT TCGAAAGTTT
    GACAGTGTAG CTTTTGGAGA AAGTCAAAGT GAGGATGAAC AATTTGAAAA TGACTTAGAG
    ACAGATCCAC CCAACTGGCA GCAGCTTGTT AGTCGAGAAG TGTTACTGGG ACTAAAACCT
    TGTGAAATCA AAAGACAGGA AGTGATTAAT GAATTGTTCT ACACTGAAAG AGCTCATGTT
    CGAACACTGA AGGTTCTTGA TCAAGTGTTC TATCAGCGAG TATCCAGAGA AGGAATTCTG
    TCACCCTCAG AGCTACGGAA AATTTTTTCA AACTTGGAAG ATATTCTTCA ACTTCATATT
    GGATTGAATG AACAAATGAA GGCTGTTCGA AAGAGAAATG AGACCTCTGT TATCGATCAG
    ATTGGGGAAG ATTTGCTGAC ATGGTTCAGC GGACCAGGAG AGGAGAAATT GAAACATGCT
    GCTGCTACCT TTTGCAGTAA CCAACCTTTC GCCCTGGAAA TGATCAAATC TCGTCAGAAA
    AAGGATTCTC GATTTCAGAC TTTTGTGCAA GATGCTGAAA GTAATCCACT GTGTCGTCGT
    CTTCAACTGA AGGATATTAT TCCCACTCAA ATGCAAAGGC TTACTAAGTA CCCACTTCTG
    TTGGATAATA TTGCCAAATA CACAGAATGG CCAACAGAAA GGGAGAAGGT GAAGAAAGCT
    GCAGATCACT GTCGTCAGAT CTTAAATTAT GTAAATCAGG CTGTCAAGGA GGCAGAAAAC
    AAGCAGCGCC TAGAAGATTA TCAGCGTCGC CTTGATACCT CCAGCCTGAA GTTGTCAGAG
    TACCCAAATG TTGAAGAGCT CAGGAATTTG GATTTAACAA AAAGGAAGAT GATTCATGAA
    GGGCCATTGG TTTGGAAGGT GAATAGAGAT AAAACTATTG ATTTATACAC GTTGCTGCTG
    GAAGACATTC TTGTATTGTT ACAAAAGCAG GATGATAGAC TGGTTTTAAG GTGTCATAGT
    AAGATTCTGG CATCTACAGC TGATAGCAAA CACACGTTTA GCCCTGTCAT TAAGTTGAGT
    ACAGTGTTGG TTCGACAAGT GGCAACAGAT AACAAAGCTT TATTCGTCAT TTCCATGTCA
    GACAATGGCG CTCAGATTTA TGAACTGGTG GCACAGACAG TTTCTGAAAA GACTGTCTGG
    CAGGACCTAA TCTGTCGGAT GGCTGCATCA GTGAAGGAGC AATCCACAAA GCCAATTCCA
    TTACCACAGT CAACACCTGG CGAAGGAGAT AATGATGAAG AAGATCCTTC AAAATTAAAA
    GAGGAGCAGC ATGGCATTTC AGTCACTGGT TTGCAGAGTC CAGACAGAGA TTTGGGATTA
    GAATCTACCT TAATATCGTC AAAACCTCAG TCTCATTCAC TGAGTACCTC TGGGAAATCA
    GAGGTACGTG ATCTGTTTGT GGCTGAGAGA CAGTTTGCAA AGGAACAACA TACAGATGGG
    ACACTAAAGG AAGTTGGAGA AGATTATCAA ATCGCAATCC CAGATTCACA CCTGCCTGTC
    TCAGAAGAAC GGTGGGCATT GGATGCACTA AGAAATTTGG GTTTGTTGAA GCAGTTGCTG
    GTGCAACAGC TAGGTTTGAC TGAGAAGAGC GTTCAGGAAG ACTGGCAACA TTTCCCAAGA
    TACAGAACAG CCTCTCAGGG GCCGCAGACA GACAGTGTCA TCCAGAACTC TGAAAATATT
    AAGGCCTATC ATTCTGGTGA AGGACATATG CCCTTTAGAA CTGGAACTGG TGACATTGCA
    ACTTGTTACA GTCCACGGAC TTCAACTGAA TCTTTTGCTC CACGGGATTC AGTGGGACTG
    GCACCCCAGG ATAGCCAGGC AAGTAACATT TTAGTAATGG ACCACATGAT TATGACCCCA
    GAGATGCCTA CCATGGAGCC AGAAGGGGGT CTTGATGACA GTGGAGAGCA CTTTTTTGAT
    GCCCGTGAAG CACATAGTGA TGAGAATCCA TCAGAAGGTG ATGGAGCAGT TAACAAGGAA
    GAGAAGGATG TTAATTTACG CATCTCAGGA AACTATTTGA TCCTTGATGG CTATGACCCA
    GTGCAGGAGA GTTCCACAGA TGAGGAGGTT GCTTCCTCAC TTACCCTGCA GCCCATGACA
    GGCATCCCTG CTGTGGAATC CACCCACCAG CAGCAACATT CTCCTCAGAA TACTCACTCC
    GATGGGGCAA TTTCACCATT CACCCCCGAA TTTCTGGTCC AGCAGCGCTG GGGAGCTATG
    GAGTATTCCT GTTTTGAGAT CCAGAGTCCC TCCTCTTGTG CAGATTCACA GAGCCAGATC
    ATGGAGTACA TTCATAAGAT AGAGGCTGAC CTTGAACACT TAAAGAAGGT GGAGGAAAGT
    TACACCATTC TTTGCCAAAG GCTGGCTGGA TCAGCCCTCA CAGACAAGCA CTCAGATAAA
    AGTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_011541022.1
    CDS586..4911
    Translation

    Target ORF information:

    RefSeq Version XM_011542720.2
    Organism Homo sapiens(human)
    Definition Homo sapiens Rho guanine nucleotide exchange factor 12 (ARHGEF12), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_011542720.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    ATGCGGGCTG GAGTACAGAC AGGTGATCGA ATCATCAAGG TGAATGGAAC TCTGGTGACT 
    CATTCAAATC ATCTGGAGGT GGTGAAGCTA ATCAAATCTG GTTCCTATGT AGCTCTCACT
    GTTCAGGGAC GCCCACCTGG GTCGCCCCAG ATTCCACTTG CCGACTCTGA AGTAGAGCCG
    TCAGTCATTG GACATATGTC TCCCATCATG ACATCTCCTC ATTCACCTGG AGCATCTGGG
    AATATGGAGA GAATCACTAG TCCTGTGCTC ATGGGGGAGG AAAACAATGT GGTTCATAAC
    CAGAAAGTAG AAATTCTGAG AAAAATGTTA CAGAAAGAAC AGGAACGGCT ACAGTTATTG
    CAGGAAGATT ACAACCGAAC ACCTGCCCAA AGATTGCTAA AAGAGATCCA AGAGGCCAAG
    AAACACATTC CTCAGCTGCA AGAGCAGTTA TCCAAAGCCA CAGGCTCTGC TCAGGATGGA
    GCTGTAGTTA CACCCTCCAG ACCTTTAGGG GACACCCTAA CAGTCAGTGA GGCAGAAACA
    GATCCTGGAG ATGTACTGGG CAGGACTGAC TGTAGCAGTG GAGATGCTTC TCGGCCCAGT
    AGTGACAATG CAGATAGTCC CAAGAGTGGC CCAAAAGAGA GAATTTATCT AGAGGAAAAC
    CCAGAGAAAA GTGAAACAAT TCAGGACACT GACACTCAAT CACTTGTCGG AAGTCCCTCA
    ACCCGTATAG CACCTCATAT TATTGGAGCA GAAGATGATG ATTTTGGTAC TGAACATGAA
    CAGATCAATG GACAGTGCAG CTGTTTCCAG AGCATTGAAT TACTAAAATC TCGCCCGGCT
    CATTTGGCTG TTTTCTTACA CCATGTAGTT TCACAATTTG ACCCTGCGAC TTTGCTCTGT
    TATCTCTATT CAGACCTGTA TAAACATACC AATTCCAAAG AAACTCGTCG CATCTTCCTT
    GAGTTTCATC AGTTCTTTCT AGATCGATCA GCACACCTGA AAGTTTCTGT TCCTGATGAA
    ATGTCTGCAG ATCTAGAAAA GAGAAGACCT GAGCTCATTC CTGAGGATCT GCATCGCCAC
    TATATCCAAA CTATGCAAGA AAGAGTCCAT CCAGAAGTTC AAAGGCACTT AGAAGATTTT
    CGGCAGAAAC GTAGTATGGG ACTGACCTTG GCTGAAAGCG AGCTGACTAA ACTTGATGCA
    GAGCGAGACA AGGACCGATT GACTTTGGAG AAGGAGCGGA CATGTGCAGA ACAGATTGTT
    GCCAAAATTG AAGAAGTATT GATGACTGCT CAGGCTGTAG AGGAAGATAA GAGCTCCACC
    ATGCAGTATG TTATTCTCAT GTATATGAAG CATTTGGGAG TAAAAGTGAA AGAGCCTCGA
    AATTTGGAGC ACAAACGGGG TCGGATTGGA TTTCTTCCCA AAATCAAGCA AAGTATGAAG
    AAAGATAAAG AAGGGGAAGA AAAAGGGAAG CGAAGAGGAT TCCCCAGCAT CCTGGGACCC
    CCACGGAGAC CAAGCCGTCA TGACAACAGT GCAATTGGCA GAGCCATGGA ACTACAGAAG
    GCGCGCCACC CTAAGCACTT ATCCACACCC TCATCTGTGA GTCCTGAACC TCAGGACTCT
    GCCAAGTTGC GCCAGAGTGG GTTAGCAAAT GAAGGAACAG ACGCTGGATA CCTGCCTGCC
    AATTCCATGT CTTCTGTAGC TTCAGGGGCC TCTTTTTCCC AGGAAGGAGG GAAAGAGAAT
    GATACAGGAT CAAAGCAAGT TGGAGAAACA TCAGCACCTG GAGACACCTT AGATGGCACA
    CCTCGTACTC TCAATACTGT CTTTGATTTC CCACCACCTC CATTAGACCA AGTGCAGGAG
    GAGGAATGTG AAGTAGAAAG GGTGACTGAA CATGGGACAC CAAAGCCCTT TCGAAAGTTT
    GACAGTGTAG CTTTTGGAGA AAGTCAAAGT GAGGATGAAC AATTTGAAAA TGACTTAGAG
    ACAGATCCAC CCAACTGGCA GCAGCTTGTT AGTCGAGAAG TGTTACTGGG ACTAAAACCT
    TGTGAAATCA AAAGACAGGA AGTGATTAAT GAATTGTTCT ACACTGAAAG AGCTCATGTT
    CGAACACTGA AGGTTCTTGA TCAAGTGTTC TATCAGCGAG TATCCAGAGA AGGAATTCTG
    TCACCCTCAG AGCTACGGAA AATTTTTTCA AACTTGGAAG ATATTCTTCA ACTTCATATT
    GGATTGAATG AACAAATGAA GGCTGTTCGA AAGAGAAATG AGACCTCTGT TATCGATCAG
    ATTGGGGAAG ATTTGCTGAC ATGGTTCAGC GGACCAGGAG AGGAGAAATT GAAACATGCT
    GCTGCTACCT TTTGCAGTAA CCAACCTTTC GCCCTGGAAA TGATCAAATC TCGTCAGAAA
    AAGGATTCTC GATTTCAGAC TTTTGTGCAA GATGCTGAAA GTAATCCACT GTGTCGTCGT
    CTTCAACTGA AGGATATTAT TCCCACTCAA ATGCAAAGGC TTACTAAGTA CCCACTTCTG
    TTGGATAATA TTGCCAAATA CACAGAATGG CCAACAGAAA GGGAGAAGGT GAAGAAAGCT
    GCAGATCACT GTCGTCAGAT CTTAAATTAT GTAAATCAGG CTGTCAAGGA GGCAGAAAAC
    AAGCAGCGCC TAGAAGATTA TCAGCGTCGC CTTGATACCT CCAGCCTGAA GTTGTCAGAG
    TACCCAAATG TTGAAGAGCT CAGGAATTTG GATTTAACAA AAAGGAAGAT GATTCATGAA
    GGGCCATTGG TTTGGAAGGT GAATAGAGAT AAAACTATTG ATTTATACAC GTTGCTGCTG
    GAAGACATTC TTGTATTGTT ACAAAAGCAG GATGATAGAC TGGTTTTAAG GTGTCATAGT
    AAGATTCTGG CATCTACAGC TGATAGCAAA CACACGTTTA GCCCTGTCAT TAAGTTGAGT
    ACAGTGTTGG TTCGACAAGT GGCAACAGAT AACAAAGCTT TATTCGTCAT TTCCATGTCA
    GACAATGGCG CTCAGATTTA TGAACTGGTG GCACAGACAG TTTCTGAAAA GACTGTCTGG
    CAGGACCTAA TCTGTCGGAT GGCTGCATCA GTGAAGGAGC AATCCACAAA GCCAATTCCA
    TTACCACAGT CAACACCTGG CGAAGGAGAT AATGATGAAG AAGATCCTTC AAAATTAAAA
    GAGGAGCAGC ATGGCATTTC AGTCACTGGT TTGCAGAGTC CAGACAGAGA TTTGGGATTA
    GAATCTACCT TAATATCGTC AAAACCTCAG TCTCATTCAC TGAGTACCTC TGGGAAATCA
    GAGGTACGTG ATCTGTTTGT GGCTGAGAGA CAGTTTGCAA AGGAACAACA TACAGATGGG
    ACACTAAAGG AAGTTGGAGA AGATTATCAA ATCGCAATCC CAGATTCACA CCTGCCTGTC
    TCAGAAGAAC GGTGGGCATT GGATGCACTA AGAAATTTGG GTTTGTTGAA GCAGTTGCTG
    GTGCAACAGC TAGGTTTGAC TGAGAAGAGC GTTCAGGAAG ACTGGCAACA TTTCCCAAGA
    TACAGAACAG CCTCTCAGGG GCCGCAGACA GACAGTGTCA TCCAGAACTC TGAAAATATT
    AAGGCCTATC ATTCTGGTGA AGGACATATG CCCTTTAGAA CTGGAACTGG TGACATTGCA
    ACTTGTTACA GTCCACGGAC TTCAACTGAA TCTTTTGCTC CACGGGATTC AGTGGGACTG
    GCACCCCAGG ATAGCCAGGC AAGTAACATT TTAGTAATGG ACCACATGAT TATGACCCCA
    GAGATGCCTA CCATGGAGCC AGAAGGGGGT CTTGATGACA GTGGAGAGCA CTTTTTTGAT
    GCCCGTGAAG CACATAGTGA TGAGAATCCA TCAGAAGGTG ATGGAGCAGT TAACAAGGAA
    GAGAAGGATG TTAATTTACG CATCTCAGGA AACTATTTGA TCCTTGATGG CTATGACCCA
    GTGCAGGAGA GTTCCACAGA TGAGGAGGTT GCTTCCTCAC TTACCCTGCA GCCCATGACA
    GGCATCCCTG CTGTGGAATC CACCCACCAG CAGCAACATT CTCCTCAGAA TACTCACTCC
    GATGGGGCAA TTTCACCATT CACCCCCGAA TTTCTGGTCC AGCAGCGCTG GGGAGCTATG
    GAGTATTCCT GTTTTGAGAT CCAGAGTCCC TCCTCTTGTG CAGATTCACA GAGCCAGATC
    ATGGAGTACA TTCATAAGAT AGAGGCTGAC CTTGAACACT TAAAGAAGGT GGAGGAAAGT
    TACACCATTC TTTGCCAAAG GCTGGCTGGA TCAGCCCTCA CAGACAAGCA CTCAGATAAA
    AGTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

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