TPSD1 cDNA ORF clone, Homo sapiens(human)
| Gene Symbol | TPSD1 |
| Entrez Gene ID | 23430 |
| Full Name | tryptase delta 1 |
| Synonyms | MCP7-LIKE,MCP7L1,MMCP-7L |
| General protein information |
|
| Gene Type | protein-coding |
| Organism | Homo sapiens(human) |
| Genome | |
| Summary | Tryptases comprise a family of trypsin-like serine proteases, the peptidase family S1. Tryptases are enzymatically active only as heparin-stabilized tetramers, and they are resistant to all known endogenous proteinase inhibitors. Several tryptase genes are clustered on chromosome 16p13.3. These genes are characterized by several distinct features. They have a highly conserved 3' UTR and contain tandem repeat sequences at the 5' flank and 3' UTR which are thought to play a role in regulation of the mRNA stability. Although this gene may be an exception, most of the tryptase genes have an intron immediately upstream of the initiator Met codon, which separates the site of transcription initiation from protein coding sequence. This feature is characteristic of tryptases but is unusual in other genes. Tryptases have been implicated as mediators in the pathogenesis of asthma and other allergic and inflammatory disorders. This gene was once considered to be a pseudogene, although it is now believed to be a functional gene that encodes a protein. [provided by RefSeq, Jul 2008]. |
| Disorder MIM: |
mRNA and Protein(s)
| mRNA | Protein | Name |
|---|---|---|
| NM_012217.3 | NP_036349.1 | tryptase delta precursor |
| NM_012217.2 | NP_036349.1 | tryptase delta precursor |
Related articles in PubMed
Human subjects are protected from mast cell tryptase deficiency despite frequent inheritance of loss-of-function mutations.
Trivedi NN, Tamraz B, Chu C, Kwok PY, Caughey GH
The Journal of allergy and clinical immunology124(5)1099-105.e1-4(2009 Nov)
Alternate mRNA splicing in multiple human tryptase genes is predicted to regulate tetramer formation.
Jackson NE, Wang HW, Bryant KJ, McNeil HP, Husain A, Liu K, Tedla N, Thomas PS, King GC, Hettiaratchi A, Cairns J, Hunt JE
The Journal of biological chemistry283(49)34178-87(2008 Dec)
Chimerism, point mutation, and truncation dramatically transformed mast cell delta-tryptases during primate evolution.
Trivedi NN, Raymond WW, Caughey GH
The Journal of allergy and clinical immunology121(5)1262-8(2008 May)
Mast cell alpha and beta tryptases changed rapidly during primate speciation and evolved from gamma-like transmembrane peptidases in ancestral vertebrates.
Trivedi NN, Tong Q, Raman K, Bhagwandin VJ, Caughey GH
Journal of immunology (Baltimore, Md. : 1950)179(9)6072-9(2007 Nov)
Tryptase genetics and anaphylaxis.
Caughey GH
The Journal of allergy and clinical immunology117(6)1411-4(2006 Jun)
GeneRIFs: Gene References Into Functions What's a GeneRIF?
Delta tryptase transcripts have been detected in a wide variety of tissues including lung, heart, stomach, spleen, skin, and colon, and a recombinant form is proteolytically active.
Title: Delta tryptase is expressed in multiple human tissues, and a recombinant form has proteolytic activity.
The following TPSD1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the TPSD1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.
| CloneID | OHu06112 |
|
| Clone ID Related Accession (Same CDS sequence) | NM_012217.3 , NM_012217.2 | |
| Accession Version | NM_012217.3 Latest version! | Documents for ORF clone product in default vector |
| Sequence Information | ORF Nucleotide Sequence (Length: 729bp) Protein sequence SNP |
|
| Vector | pcDNA3.1+/C-(K)DYK or customized vector |  User Manual |
| Clone information | Clone Map | MSDS |
| Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
| ORF Insert Method | CloneEZ™ Seamless cloning technology | |
| Insert Structure | linear | |
| Update Date | 2019-09-21 | |
| Organism | Homo sapiens(human) | |
| Product | tryptase delta precursor | |
| Comment | Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC120498.2. On Nov 26, 2018 this sequence version replaced NM_012217.2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC069143.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968540, SAMEA2142348 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000211076.5/ ENSP00000211076.3 RefSeq Select criteria :: based on single protein-coding transcript ##RefSeq-Attributes-END## COMPLETENESS: full length. | |
1 | ATGCTCCTCC TTGCTCCCCA GATGCTGAGC CTGCTGCTGC TGGCGCTGCC CGTCCTGGCG |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| RefSeq | NP_036349.1 |
| CDS | 213..941 |
| Translation |
Target ORF information:
Target ORF information:
|
 NM_012217.3 |
ORF Insert Sequence:
1 | ATGCTCCTCC TTGCTCCCCA GATGCTGAGC CTGCTGCTGC TGGCGCTGCC CGTCCTGGCG |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| CloneID | OHu06112 |
|
| Clone ID Related Accession (Same CDS sequence) | NM_012217.3 , NM_012217.2 | |
| Accession Version | NM_012217.2 | Documents for ORF clone product in default vector |
| Sequence Information | ORF Nucleotide Sequence (Length: 729bp) Protein sequence SNP |
|
| Vector | pcDNA3.1+/C-(K)DYK or customized vector | User Manual |
| Clone information | Clone Map | MSDS |
| Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
| ORF Insert Method | CloneEZ™ Seamless cloning technology | |
| Insert Structure | linear | |
| Update Date | 2017-12-26 | |
| Organism | Homo sapiens(human) | |
| Product | tryptase delta precursor | |
| Comment | Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AF098327.1. On Jul 8, 2004 this sequence version replaced NM_012217.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC069143.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968540, SAMEA2142348 [ECO:0000348] ##Evidence-Data-END## | |
1 | ATGCTCCTCC TTGCTCCCCA GATGCTGAGC CTGCTGCTGC TGGCGCTGCC CGTCCTGGCG |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| RefSeq | NP_036349.1 |
| CDS | 10..738 |
| Translation |
Target ORF information:
Target ORF information:
|
NM_012217.2 |
ORF Insert Sequence:
1 | ATGCTCCTCC TTGCTCCCCA GATGCTGAGC CTGCTGCTGC TGGCGCTGCC CGTCCTGGCG |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
 |
Human subjects are protected from mast cell tryptase deficiency despite frequent inheritance of loss-of-function mutations. |
|
Alternate mRNA splicing in multiple human tryptase genes is predicted to regulate tetramer formation. |
|
Chimerism, point mutation, and truncation dramatically transformed mast cell delta-tryptases during primate evolution. |
|
Mast cell alpha and beta tryptases changed rapidly during primate speciation and evolved from gamma-like transmembrane peptidases in ancestral vertebrates. |
|
Tryptase genetics and anaphylaxis. |