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HPGD cDNA ORF clone, Homo sapiens(Human)

Gene Symbol HPGD
Entrez Gene ID 3248
Full Name 15-hydroxyprostaglandin dehydrogenase
Synonyms 15-PGDH,PGDH,PGDH1,PHOAR1,SDR36C1
General protein information
Preferred Names

15-hydroxyprostaglandin dehydrogenase

Names

15-hydroxyprostaglandin dehydrogenase [NAD(+)]
15-hydroxyprostaglandin dehydrogenase (NAD(+))
NAD+-dependent 15-hydroxyprostaglandin dehydrogenase
hydroxyprostaglandin dehydrogenase 15-(NAD)
prostaglandin dehydrogenase 1
short chain dehydrogenase/reductase family 36C member 1

Gene Type protein-coding
Organism Homo sapiens(Human)
Genome

4

4q34.1

Summary This gene encodes a member of the short-chain nonmetalloenzyme alcohol dehydrogenase protein family. The encoded enzyme is responsible for the metabolism of prostaglandins, which function in a variety of physiologic and cellular processes such as inflammation. Mutations in this gene result in primary autosomal recessive hypertrophic osteoarthropathy and cranioosteoarthropathy. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Mar 2009].
Disorder MIM:

601688

Disorder Html: Cranioosteoarthropathy, 259100 (3); Hypertrophic osteoarthropathy,

mRNA and Protein(s)

mRNA Protein Name
XM_011531907.1 XP_011530209.1 15-hydroxyprostaglandin dehydrogenase [NAD(+)] isoform X1
NM_001256306.1 NP_001243235.1 15-hydroxyprostaglandin dehydrogenase [NAD(+)] isoform 5
NM_001256307.1 NP_001243236.1 15-hydroxyprostaglandin dehydrogenase [NAD(+)] isoform 3
NM_001145816.2 NP_001139288.1 15-hydroxyprostaglandin dehydrogenase [NAD(+)] isoform 2
NM_000860.5 NP_000851.2 15-hydroxyprostaglandin dehydrogenase [NAD(+)] isoform 1
NM_001256305.1 NP_001243234.1 15-hydroxyprostaglandin dehydrogenase [NAD(+)] isoform 4
NM_001256301.1 NP_001243230.1 15-hydroxyprostaglandin dehydrogenase [NAD(+)] isoform 3



Homo sapiens (human) HPGD NP_000851.2
Gallus gallus (chicken) HPGD NP_001264713.1
Anopheles gambiae (African malaria mosquito) AgaP_AGAP001753 XP_321329.4
Macaca mulatta (rhesus monkey) HPGD XP_001087957.1
Mus musculus (house mouse) Hpgd NP_032304.2
Rattus norvegicus (Norway rat) Hpgd NP_077366.2
Xenopus tropicalis (tropical clawed frog) hpgd NP_001007992.1
Pan troglodytes (chimpanzee) HPGD XP_517542.2
Canis lupus familiaris (dog) HPGD XP_543199.1
Bos taurus (cattle) HPGD NP_001029591.1
Danio rerio (zebrafish) LOC565975 XP_694331.5


Related articles in PubMed

HSP90 Inhibition Suppresses PGE2 Production via Modulating COX-2 and 15-PGDH Expression in HT-29 Colorectal Cancer Cells.
Mohammadi A, Yaghoobi MM, Gholamhoseinian Najar A, Kalantari-Khandani B, Sharifi H, Saravani M
Inflammation39(3)1116-23(2016 Jun)

A Common Mutation and a Novel Mutation in the HPGD Gene in Nine Patients with Primary Hypertrophic Osteoarthropathy.
Yuan L, Chen L, Liao RX, Lin YY, Jiang Y, Wang O, Li M, Xing XP, Pang QQ, Jiajue R, Xia WB
Calcified tissue international97(4)336-42(2015 Oct)

Expression of 15-Hydroxyprostaglandin Dehydrogenase in Human Chorion Is Associated with Peroxisome Proliferator-Activated Receptor Isoform Expression in Term Labor.
He P, Li Y, Ding X, Sun Q, Huang Y, Gu H, Ni X
The American journal of pathology185(7)1981-90(2015 Jul)

15-PGDH expression as a predictive factor response to neoadjuvant chemotherapy in advanced gastric cancer.
Hu M, Li K, Maskey N, Xu Z, Peng C, Tian S, Li Y, Yang G
International journal of clinical and experimental pathology8(6)6910-8(2015)

Expression and Cellular Localization of 15-Hydroxy-Prostaglandin-Dehydrogenase in Abdominal Aortic Aneurysm.
Solà-Villà D, Dilmé JF, Rodríguez C, Soto B, Vila L, Escudero JR, Martínez-González J, Camacho M
PloS one10(8)e0136201(2015)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

inhibitory effects of 17-AAG on PGE2 levels in HT-29 colorectal cancer cells were mediated through modulating COX-2 and 15-PGDH expression.
Title: HSP90 Inhibition Suppresses PGE2 Production via Modulating COX-2 and 15-PGDH Expression in HT-29 Colorectal Cancer Cells.

A common mutation and a novel mutation in HPGD gene were identified to be responsible for primary hypertrophic osteoarthropathy
Title: A Common Mutation and a Novel Mutation in the HPGD Gene in Nine Patients with Primary Hypertrophic Osteoarthropathy.

Peroxisome proliferator-activated receptors (PPARs) play pivotal roles in maintenance of Chorionic NAD-dependent 15-hydroxy prostaglandin dehydrogenase (PGDH) expression in chorion during human pregnancy.
Title: Expression of 15-Hydroxyprostaglandin Dehydrogenase in Human Chorion Is Associated with Peroxisome Proliferator-Activated Receptor Isoform Expression in Term Labor.

Neoadjuvant chemotherapy could increase 15-PGDH expression in advanced gastric cancer patients, and 15-PGDH may serve as a candidate prognostic biomarker of advanced GC response to therapy.
Title: 15-PGDH expression as a predictive factor response to neoadjuvant chemotherapy in advanced gastric cancer.

15-PGDH mRNA levels were significantly higher in aorta samples from patients undergoing abdominal aortic aneurysm repair than in those from healthy multiorgan donors.
Title: Expression and Cellular Localization of 15-Hydroxy-Prostaglandin-Dehydrogenase in Abdominal Aortic Aneurysm.

The following HPGD gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the HPGD cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

Online Only Promotion

Up to 50% OFF! Starting
from $49/clone

***CloneID RefSeq Accession Definition **Vector *Turnaround time Price (USD) Select
OHu16895 NM_001256301.1
Latest version!
Homo sapiens hydroxyprostaglandin dehydrogenase 15-(NAD) (HPGD), transcript variant 3, mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
$49.50-$69.30
$99.00
OHu16895 NM_001256307.1
Latest version!
Homo sapiens hydroxyprostaglandin dehydrogenase 15-(NAD) (HPGD), transcript variant 6, mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
$49.50-$69.30
$99.00
OHu20277 NM_000860.5
Latest version!
Homo sapiens hydroxyprostaglandin dehydrogenase 15-(NAD) (HPGD), transcript variant 1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
$99.50-$139.30
$199.00
OHu16712 NM_001256305.1
Latest version!
Homo sapiens hydroxyprostaglandin dehydrogenase 15-(NAD) (HPGD), transcript variant 4, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $69.30
$99.00
OHu16860 NM_001145816.2
Latest version!
Homo sapiens hydroxyprostaglandin dehydrogenase 15-(NAD) (HPGD), transcript variant 2, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $69.30
$99.00
OHu16914 NM_001256306.1
Latest version!
Homo sapiens hydroxyprostaglandin dehydrogenase 15-(NAD) (HPGD), transcript variant 5, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $69.30
$99.00
OHu58309 XM_011531907.1
Latest version!
Homo sapiens hydroxyprostaglandin dehydrogenase 15-(NAD) (HPGD), transcript variant X1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $69.30
$99.00

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2-4 Clone 40% OFF
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.


CloneID OHu16895
Clone ID Related Accession (Same CDS sequence) NM_001256307.1 , NM_001256301.1
Accession Version NM_001256307.1 Latest version! Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 438bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2017-04-09
Organism Homo sapiens(Human)
Product 15-hydroxyprostaglandin dehydrogenase [NAD(+)] isoform 3
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DC420578.1, AK300524.1, AC096751.3, BC018986.2 and AI435471.1. Transcript Variant: This variant (6) lacks a 5' exon compared to variant 1. This variant represents translation initiation at a downstream AUG compared to variant 1; the 5'-most initiation codon, as used in variant 1, is associated with a weak Kozak sequence and a truncated ORF that would render the transcript a candidate for nonsense-mediated decay (NMD). Leaky scanning may allow translation initiation at the downstream AUG, to encode an isoform (3) with a shorter N-terminus, compared to isoform 1. Variants 3 and 6 encode the same protein (isoform 3). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK300524.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END##

1
61
121
181
241
301
361
421
ATGAGTAAGC AAAATGGAGG TGAAGGCGGC ATCATTATCA ATATGTCATC TTTAGCAGGA 
CTCATGCCCG TTGCACAGCA GCCGGTTTAT TGTGCTTCAA AGCATGGCAT AGTTGGATTC
ACACGCTCAG CAGCGTTGGC TGCTAATCTT ATGAACAGTG GTGTGAGACT GAATGCCATT
TGTCCAGGCT TTGTTAACAC AGCCATCCTT GAATCAATTG AAAAAGAAGA AAACATGGGA
CAATATATAG AATATAAGGA TCATATCAAG GATATGATTA AATACTATGG AATTTTGGAC
CCACCATTGA TTGCCAATGG ATTGATAACA CTCATTGAAG ATGATGCTTT AAATGGTGCT
ATTATGAAGA TCACAACTTC TAAGGGAATT CATTTTCAAG ACTATGATAC AACTCCATTT
CAAGCAAAAA CCCAATGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001243236.1
CDS704..1141
Misc Feature(1)659..661
Misc Feature(2)<704..1102
Exon (1)1..540
Exon (2)541..664
Exon (3)665..761
Exon (4)762..838
Exon (5)839..1002
Exon (6)1003..2920
Translation

Target ORF information:

RefSeq Version NM_001256307.1
Organism Homo sapiens(Human)
Definition Homo sapiens hydroxyprostaglandin dehydrogenase 15-(NAD) (HPGD), transcript variant 6, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_001256307.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
ATGAGTAAGC AAAATGGAGG TGAAGGCGGC ATCATTATCA ATATGTCATC TTTAGCAGGA 
CTCATGCCCG TTGCACAGCA GCCGGTTTAT TGTGCTTCAA AGCATGGCAT AGTTGGATTC
ACACGCTCAG CAGCGTTGGC TGCTAATCTT ATGAACAGTG GTGTGAGACT GAATGCCATT
TGTCCAGGCT TTGTTAACAC AGCCATCCTT GAATCAATTG AAAAAGAAGA AAACATGGGA
CAATATATAG AATATAAGGA TCATATCAAG GATATGATTA AATACTATGG AATTTTGGAC
CCACCATTGA TTGCCAATGG ATTGATAACA CTCATTGAAG ATGATGCTTT AAATGGTGCT
ATTATGAAGA TCACAACTTC TAAGGGAATT CATTTTCAAG ACTATGATAC AACTCCATTT
CAAGCAAAAA CCCAATGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu16895
Clone ID Related Accession (Same CDS sequence) NM_001256307.1 , NM_001256301.1
Accession Version NM_001256301.1 Latest version! Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 438bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2017-04-09
Organism Homo sapiens(Human)
Product 15-hydroxyprostaglandin dehydrogenase [NAD(+)] isoform 3
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AK296642.1, BC018986.2 and AI435471.1. On Jan 19, 2012 this sequence version replaced NR_027332.1. Transcript Variant: This variant (3) differs in the 5' UTR and lacks a portion of the 5' coding region, compared to variant 1. These differences result in translation at a downstream start codon and an isoform (3) with a shorter N-terminus, compared to isoform 1. Variants 3 and 6 encode the same protein (isoform 3). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK296642.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END##

1
61
121
181
241
301
361
421
ATGAGTAAGC AAAATGGAGG TGAAGGCGGC ATCATTATCA ATATGTCATC TTTAGCAGGA 
CTCATGCCCG TTGCACAGCA GCCGGTTTAT TGTGCTTCAA AGCATGGCAT AGTTGGATTC
ACACGCTCAG CAGCGTTGGC TGCTAATCTT ATGAACAGTG GTGTGAGACT GAATGCCATT
TGTCCAGGCT TTGTTAACAC AGCCATCCTT GAATCAATTG AAAAAGAAGA AAACATGGGA
CAATATATAG AATATAAGGA TCATATCAAG GATATGATTA AATACTATGG AATTTTGGAC
CCACCATTGA TTGCCAATGG ATTGATAACA CTCATTGAAG ATGATGCTTT AAATGGTGCT
ATTATGAAGA TCACAACTTC TAAGGGAATT CATTTTCAAG ACTATGATAC AACTCCATTT
CAAGCAAAAA CCCAATGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001243230.1
CDS541..978
Misc Feature(1)190..192
Misc Feature(2)<541..939
Exon (1)1..270
Exon (2)271..394
Exon (3)395..501
Exon (4)502..598
Exon (5)599..675
Exon (6)676..839
Exon (7)840..2757
Translation

Target ORF information:

RefSeq Version NM_001256301.1
Organism Homo sapiens(Human)
Definition Homo sapiens hydroxyprostaglandin dehydrogenase 15-(NAD) (HPGD), transcript variant 3, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_001256301.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
ATGAGTAAGC AAAATGGAGG TGAAGGCGGC ATCATTATCA ATATGTCATC TTTAGCAGGA 
CTCATGCCCG TTGCACAGCA GCCGGTTTAT TGTGCTTCAA AGCATGGCAT AGTTGGATTC
ACACGCTCAG CAGCGTTGGC TGCTAATCTT ATGAACAGTG GTGTGAGACT GAATGCCATT
TGTCCAGGCT TTGTTAACAC AGCCATCCTT GAATCAATTG AAAAAGAAGA AAACATGGGA
CAATATATAG AATATAAGGA TCATATCAAG GATATGATTA AATACTATGG AATTTTGGAC
CCACCATTGA TTGCCAATGG ATTGATAACA CTCATTGAAG ATGATGCTTT AAATGGTGCT
ATTATGAAGA TCACAACTTC TAAGGGAATT CATTTTCAAG ACTATGATAC AACTCCATTT
CAAGCAAAAA CCCAATGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu20277
Clone ID Related Accession (Same CDS sequence) NM_000860.5
Accession Version NM_000860.5 Latest version! Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 801bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2017-04-30
Organism Homo sapiens(Human)
Product 15-hydroxyprostaglandin dehydrogenase [NAD(+)] isoform 1
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DC420578.1, BC018986.2 and AI435471.1. This sequence is a reference standard in the RefSeqGene project. On Jan 19, 2012 this sequence version replaced NM_000860.4. Transcript Variant: This variant (1) represents the longest transcript and encodes the longer isoform (1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660809.107100.1, AK058013.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
ATGCACGTGA ACGGCAAAGT GGCGCTGGTG ACCGGCGCGG CTCAGGGCAT AGGCAGAGCC 
TTTGCAGAGG CGCTGCTGCT TAAGGGCGCC AAGGTAGCGC TGGTGGATTG GAATCTTGAA
GCAGGTGTAC AGTGTAAAGC TGCCCTGGAT GAGCAGTTTG AACCTCAGAA GACTCTGTTC
ATCCAGTGCG ATGTGGCTGA CCAGCAACAA CTGAGAGACA CTTTTAGAAA AGTTGTAGAC
CACTTTGGAA GACTGGACAT TTTGGTCAAT AATGCTGGAG TGAATAATGA GAAAAACTGG
GAAAAAACTC TGCAAATTAA TTTGGTTTCT GTTATCAGTG GAACCTATCT TGGTTTGGAT
TACATGAGTA AGCAAAATGG AGGTGAAGGC GGCATCATTA TCAATATGTC ATCTTTAGCA
GGACTCATGC CCGTTGCACA GCAGCCGGTT TATTGTGCTT CAAAGCATGG CATAGTTGGA
TTCACACGCT CAGCAGCGTT GGCTGCTAAT CTTATGAACA GTGGTGTGAG ACTGAATGCC
ATTTGTCCAG GCTTTGTTAA CACAGCCATC CTTGAATCAA TTGAAAAAGA AGAAAACATG
GGACAATATA TAGAATATAA GGATCATATC AAGGATATGA TTAAATACTA TGGAATTTTG
GACCCACCAT TGATTGCCAA TGGATTGATA ACACTCATTG AAGATGATGC TTTAAATGGT
GCTATTATGA AGATCACAAC TTCTAAGGGA ATTCATTTTC AAGACTATGA TACAACTCCA
TTTCAAGCAA AAACCCAATG A

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_000851.2
CDS448..1248
Misc Feature(1)277..279
Misc Feature(2)463..1209
Misc Feature(3)463..1044
Misc Feature(4)order(481..483,487..498,553..561,634..645,718..729,763..765,853..861,898..900,910..912,994..1005,1009..1020)
Misc Feature(5)order(745..750,757..759,769..774,781..786,793..795,802..804,862..864,871..879,883..888,892..897,901..909,913..918,925..930,937..942,946..951,1000..1002,1096..1098,1183..1185,1204..1209)
Misc Feature(6)order(766..768,859..861,898..900,910..912)
Exon (1)1..540
Exon (2)541..664
Exon (3)665..771
Exon (4)772..868
Exon (5)869..945
Exon (6)946..1109
Exon (7)1110..3027
Translation

Target ORF information:

RefSeq Version NM_000860.5
Organism Homo sapiens(Human)
Definition Homo sapiens hydroxyprostaglandin dehydrogenase 15-(NAD) (HPGD), transcript variant 1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_000860.5

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
ATGCACGTGA ACGGCAAAGT GGCGCTGGTG ACCGGCGCGG CTCAGGGCAT AGGCAGAGCC 
TTTGCAGAGG CGCTGCTGCT TAAGGGCGCC AAGGTAGCGC TGGTGGATTG GAATCTTGAA
GCAGGTGTAC AGTGTAAAGC TGCCCTGGAT GAGCAGTTTG AACCTCAGAA GACTCTGTTC
ATCCAGTGCG ATGTGGCTGA CCAGCAACAA CTGAGAGACA CTTTTAGAAA AGTTGTAGAC
CACTTTGGAA GACTGGACAT TTTGGTCAAT AATGCTGGAG TGAATAATGA GAAAAACTGG
GAAAAAACTC TGCAAATTAA TTTGGTTTCT GTTATCAGTG GAACCTATCT TGGTTTGGAT
TACATGAGTA AGCAAAATGG AGGTGAAGGC GGCATCATTA TCAATATGTC ATCTTTAGCA
GGACTCATGC CCGTTGCACA GCAGCCGGTT TATTGTGCTT CAAAGCATGG CATAGTTGGA
TTCACACGCT CAGCAGCGTT GGCTGCTAAT CTTATGAACA GTGGTGTGAG ACTGAATGCC
ATTTGTCCAG GCTTTGTTAA CACAGCCATC CTTGAATCAA TTGAAAAAGA AGAAAACATG
GGACAATATA TAGAATATAA GGATCATATC AAGGATATGA TTAAATACTA TGGAATTTTG
GACCCACCAT TGATTGCCAA TGGATTGATA ACACTCATTG AAGATGATGC TTTAAATGGT
GCTATTATGA AGATCACAAC TTCTAAGGGA ATTCATTTTC AAGACTATGA TACAACTCCA
TTTCAAGCAA AAACCCAATG A

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu16712
Clone ID Related Accession (Same CDS sequence) NM_001256305.1
Accession Version NM_001256305.1 Latest version! Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 432bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2017-04-09
Organism Homo sapiens(Human)
Product 15-hydroxyprostaglandin dehydrogenase [NAD(+)] isoform 4
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DC420578.1, U63296.1, AK058013.1, BC018986.2 and AI435471.1. Transcript Variant: This variant (4) lacks two alternate exons in the 3' coding region, which results in a frameshift and early stop codon, compared to variant 1. It encodes isoform 4, which is shorter and contains a distinct C-terminus, compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: U63296.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2161836 [ECO:0000348] ##Evidence-Data-END##

1
61
121
181
241
301
361
421
ATGCACGTGA ACGGCAAAGT GGCGCTGGTG ACCGGCGCGG CTCAGGGCAT AGGCAGAGCC 
TTTGCAGAGG CGCTGCTGCT TAAGGGCGCC AAGGTAGCGC TGGTGGATTG GAATCTTGAA
GCAGGTGTAC AGTGTAAAGC TGCCCTGGAT GAGCAGTTTG AACCTCAGAA GACTCTGTTC
ATCCAGTGCG ATGTGGCTGA CCAGCAACAA CTGAGAGACA CTTTTAGAAA AGTTGTAGAC
CACTTTGGAA GACTGGACAT TTTGGTCAAT AATGCTGGAG TGAATAATGA GAAAAACTGG
GAAAAAACTC TGCAAATTAA TTTGGTTTCT GTTATCAGTG GAACCTATCT TGGTTTGGAT
TACATGAGTA AGCAAAATGG AGGTGAAGGC GGCATCATTA TCAATATGTC ATCTTTAGCA
GCCCACCATT GA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001243234.1
CDS448..879
Misc Feature(1)277..279
Misc Feature(2)463..>876
Exon (1)1..540
Exon (2)541..664
Exon (3)665..771
Exon (4)772..868
Exon (5)869..2786
Translation

Target ORF information:

RefSeq Version NM_001256305.1
Organism Homo sapiens(Human)
Definition Homo sapiens hydroxyprostaglandin dehydrogenase 15-(NAD) (HPGD), transcript variant 4, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_001256305.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
ATGCACGTGA ACGGCAAAGT GGCGCTGGTG ACCGGCGCGG CTCAGGGCAT AGGCAGAGCC 
TTTGCAGAGG CGCTGCTGCT TAAGGGCGCC AAGGTAGCGC TGGTGGATTG GAATCTTGAA
GCAGGTGTAC AGTGTAAAGC TGCCCTGGAT GAGCAGTTTG AACCTCAGAA GACTCTGTTC
ATCCAGTGCG ATGTGGCTGA CCAGCAACAA CTGAGAGACA CTTTTAGAAA AGTTGTAGAC
CACTTTGGAA GACTGGACAT TTTGGTCAAT AATGCTGGAG TGAATAATGA GAAAAACTGG
GAAAAAACTC TGCAAATTAA TTTGGTTTCT GTTATCAGTG GAACCTATCT TGGTTTGGAT
TACATGAGTA AGCAAAATGG AGGTGAAGGC GGCATCATTA TCAATATGTC ATCTTTAGCA
GCCCACCATT GA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu16860
Clone ID Related Accession (Same CDS sequence) NM_001145816.2
Accession Version NM_001145816.2 Latest version! Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 537bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2017-04-09
Organism Homo sapiens(Human)
Product 15-hydroxyprostaglandin dehydrogenase [NAD(+)] isoform 2
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DC420578.1, X82460.1, AK300940.1, BC018986.2 and AI435471.1. On Jan 19, 2012 this sequence version replaced NM_001145816.1. Transcript Variant: This variant (2) lacks an alternate exon in the 3' coding region resulting in a frameshift, compared to variant 1. The resulting isoform (2) has a shorter and distinct C-terminus, compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK300940.1, X82460.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968540, SAMEA2142586 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
ATGCACGTGA ACGGCAAAGT GGCGCTGGTG ACCGGCGCGG CTCAGGGCAT AGGCAGAGCC 
TTTGCAGAGG CGCTGCTGCT TAAGGGCGCC AAGGTAGCGC TGGTGGATTG GAATCTTGAA
GCAGGTGTAC AGTGTAAAGC TGCCCTGGAT GAGCAGTTTG AACCTCAGAA GACTCTGTTC
ATCCAGTGCG ATGTGGCTGA CCAGCAACAA CTGAGAGACA CTTTTAGAAA AGTTGTAGAC
CACTTTGGAA GACTGGACAT TTTGGTCAAT AATGCTGGAG TGAATAATGA GAAAAACTGG
GAAAAAACTC TGCAAATTAA TTTGGTTTCT GTTATCAGTG GAACCTATCT TGGTTTGGAT
TACATGAGTA AGCAAAATGG AGGTGAAGGC GGCATCATTA TCAATATGTC ATCTTTAGCA
GGACTCATGC CCGTTGCACA GCAGCCGGTT TATTGTGCTT CAAAGCATGG CATAGTTGGA
TTCACACGCT CAGCAGCGCC CACCATTGAT TGCCAATGGA TTGATAACAC TCATTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001139288.1
CDS448..984
Misc Feature(1)277..279
Misc Feature(2)463..>951
Misc Feature(3)463..945
Misc Feature(4)order(745..750,757..759,769..774,781..786,793..795,802..804,862..864,871..879,883..888,892..897,901..909,913..918,925..930,937..942,946..951)
Misc Feature(5)order(766..768,859..861,898..900,910..912)
Exon (1)1..540
Exon (2)541..664
Exon (3)665..771
Exon (4)772..868
Exon (5)869..945
Exon (6)946..2863
Translation

Target ORF information:

RefSeq Version NM_001145816.2
Organism Homo sapiens(Human)
Definition Homo sapiens hydroxyprostaglandin dehydrogenase 15-(NAD) (HPGD), transcript variant 2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_001145816.2

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
ATGCACGTGA ACGGCAAAGT GGCGCTGGTG ACCGGCGCGG CTCAGGGCAT AGGCAGAGCC 
TTTGCAGAGG CGCTGCTGCT TAAGGGCGCC AAGGTAGCGC TGGTGGATTG GAATCTTGAA
GCAGGTGTAC AGTGTAAAGC TGCCCTGGAT GAGCAGTTTG AACCTCAGAA GACTCTGTTC
ATCCAGTGCG ATGTGGCTGA CCAGCAACAA CTGAGAGACA CTTTTAGAAA AGTTGTAGAC
CACTTTGGAA GACTGGACAT TTTGGTCAAT AATGCTGGAG TGAATAATGA GAAAAACTGG
GAAAAAACTC TGCAAATTAA TTTGGTTTCT GTTATCAGTG GAACCTATCT TGGTTTGGAT
TACATGAGTA AGCAAAATGG AGGTGAAGGC GGCATCATTA TCAATATGTC ATCTTTAGCA
GGACTCATGC CCGTTGCACA GCAGCCGGTT TATTGTGCTT CAAAGCATGG CATAGTTGGA
TTCACACGCT CAGCAGCGCC CACCATTGAT TGCCAATGGA TTGATAACAC TCATTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu16914
Clone ID Related Accession (Same CDS sequence) NM_001256306.1
Accession Version NM_001256306.1 Latest version! Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 597bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2017-04-09
Organism Homo sapiens(Human)
Product 15-hydroxyprostaglandin dehydrogenase [NAD(+)] isoform 5
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DC420578.1, AK300125.1, BC018986.2 and AI435471.1. Transcript Variant: This variant (5) lacks two alternate in-frame exons in the coding region, compared to variant 1. It encodes isoform 5, which is shorter but contains the same N- and C-termini, compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK300125.1, AU139547.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END##

1
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121
181
241
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361
421
481
541
ATGCACGTGA ACGGCAAAGT GGCGCTGGTG ACCGGCGCGG CTCAGGGCAT AGGCAGAGCC 
TTTGCAGAGG CGCTGCTGCT TAAGGGCGCC AAGGTAGCGC TGGTGGATTG GAATCTTGAA
GCAGGTGTAC AGTGTAAAGC TGCCCTGGAT GAGCAGTTTG AACCTCAGAA GACTCTGTTC
ATCCAGTGCG ATGTGGCTGA CCAGCAACAA CTGAGAGGAC TCATGCCCGT TGCACAGCAG
CCGGTTTATT GTGCTTCAAA GCATGGCATA GTTGGATTCA CACGCTCAGC AGCGTTGGCT
GCTAATCTTA TGAACAGTGG TGTGAGACTG AATGCCATTT GTCCAGGCTT TGTTAACACA
GCCATCCTTG AATCAATTGA AAAAGAAGAA AACATGGGAC AATATATAGA ATATAAGGAT
CATATCAAGG ATATGATTAA ATACTATGGA ATTTTGGACC CACCATTGAT TGCCAATGGA
TTGATAACAC TCATTGAAGA TGATGCTTTA AATGGTGCTA TTATGAAGAT CACAACTTCT
AAGGGAATTC ATTTTCAAGA CTATGATACA ACTCCATTTC AAGCAAAAAC CCAATGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001243235.1
CDS448..1044
Misc Feature(1)277..279
Misc Feature(2)463..1005
Misc Feature(3)463..840
Exon (1)1..540
Exon (2)541..664
Exon (3)665..741
Exon (4)742..905
Exon (5)906..2823
Translation

Target ORF information:

RefSeq Version NM_001256306.1
Organism Homo sapiens(Human)
Definition Homo sapiens hydroxyprostaglandin dehydrogenase 15-(NAD) (HPGD), transcript variant 5, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_001256306.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
ATGCACGTGA ACGGCAAAGT GGCGCTGGTG ACCGGCGCGG CTCAGGGCAT AGGCAGAGCC 
TTTGCAGAGG CGCTGCTGCT TAAGGGCGCC AAGGTAGCGC TGGTGGATTG GAATCTTGAA
GCAGGTGTAC AGTGTAAAGC TGCCCTGGAT GAGCAGTTTG AACCTCAGAA GACTCTGTTC
ATCCAGTGCG ATGTGGCTGA CCAGCAACAA CTGAGAGGAC TCATGCCCGT TGCACAGCAG
CCGGTTTATT GTGCTTCAAA GCATGGCATA GTTGGATTCA CACGCTCAGC AGCGTTGGCT
GCTAATCTTA TGAACAGTGG TGTGAGACTG AATGCCATTT GTCCAGGCTT TGTTAACACA
GCCATCCTTG AATCAATTGA AAAAGAAGAA AACATGGGAC AATATATAGA ATATAAGGAT
CATATCAAGG ATATGATTAA ATACTATGGA ATTTTGGACC CACCATTGAT TGCCAATGGA
TTGATAACAC TCATTGAAGA TGATGCTTTA AATGGTGCTA TTATGAAGAT CACAACTTCT
AAGGGAATTC ATTTTCAAGA CTATGATACA ACTCCATTTC AAGCAAAAAC CCAATGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu58309
Clone ID Related Accession (Same CDS sequence) XM_011531907.1
Accession Version XM_011531907.1 Latest version! Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 531bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2016-06-06
Organism Homo sapiens(Human)
Product 15-hydroxyprostaglandin dehydrogenase [NAD(+)] isoform X1
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000004.12) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

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121
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481
ATGCACGTGA ACGGCAAAGT GGCGCTGGTG ACCGGCGCGG CTCAGGGCAT AGGCAGAGCC 
TTTGCAGAGG CGCTGCTGCT TAAGGGCGCC AAGGTAGCGC TGGTGGATTG GAATCTTGAA
GCAGGTGTAC AGTGTAAAGC TGCCCTGGAT GAGCAGTTTG AACCTCAGAA GACTCTGTTC
ATCCAGTGCG ATGTGGCTGA CCAGCAACAA CTGAGAGACA CTTTTAGAAA AGTTGTAGAC
CACTTTGGAA GACTGGACAT TTTGGTCAAT AATGCTGGAG TGAATAATGA GAAAAACTGG
GAAAAAACTC TGCAAATTAA TTTGGTTTCT GTTATCAGTG GAACCTATCT TGGTTTGGAT
TACATGAGTA AGCAAAATGG AGGTGAAGGC GGCATCATTA TCAATATGTC ATCTTTAGCA
GGTATCTCTT CTGTATGCAG ACATTTGAAA ACCTGGCTGG CAGAAGTTCT GCCGTCTTCA
GCAGGGAGCT CCCAGGGTTA CTTTAGAACT GCTGGATTCC TTTTTCTCTA G

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_011530209.1
CDS443..973
Misc Feature(1)458..>865
Translation

Target ORF information:

RefSeq Version XM_011531907.1
Organism Homo sapiens(Human)
Definition Homo sapiens hydroxyprostaglandin dehydrogenase 15-(NAD) (HPGD), transcript variant X1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_011531907.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
ATGCACGTGA ACGGCAAAGT GGCGCTGGTG ACCGGCGCGG CTCAGGGCAT AGGCAGAGCC 
TTTGCAGAGG CGCTGCTGCT TAAGGGCGCC AAGGTAGCGC TGGTGGATTG GAATCTTGAA
GCAGGTGTAC AGTGTAAAGC TGCCCTGGAT GAGCAGTTTG AACCTCAGAA GACTCTGTTC
ATCCAGTGCG ATGTGGCTGA CCAGCAACAA CTGAGAGACA CTTTTAGAAA AGTTGTAGAC
CACTTTGGAA GACTGGACAT TTTGGTCAAT AATGCTGGAG TGAATAATGA GAAAAACTGG
GAAAAAACTC TGCAAATTAA TTTGGTTTCT GTTATCAGTG GAACCTATCT TGGTTTGGAT
TACATGAGTA AGCAAAATGG AGGTGAAGGC GGCATCATTA TCAATATGTC ATCTTTAGCA
GGTATCTCTT CTGTATGCAG ACATTTGAAA ACCTGGCTGG CAGAAGTTCT GCCGTCTTCA
GCAGGGAGCT CCCAGGGTTA CTTTAGAACT GCTGGATTCC TTTTTCTCTA G

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

book

HSP90 Inhibition Suppresses PGE2 Production via Modulating COX-2 and 15-PGDH Expression in HT-29 Colorectal Cancer Cells.
Inflammation39(3)1116-23(2016 Jun)
Mohammadi A,Yaghoobi MM,Gholamhoseinian Najar A,Kalantari-Khandani B,Sharifi H,Saravani M


book

A Common Mutation and a Novel Mutation in the HPGD Gene in Nine Patients with Primary Hypertrophic Osteoarthropathy.
Calcified tissue international97(4)336-42(2015 Oct)
Yuan L,Chen L,Liao RX,Lin YY,Jiang Y,Wang O,Li M,Xing XP,Pang QQ,Jiajue R,Xia WB


book

Expression of 15-Hydroxyprostaglandin Dehydrogenase in Human Chorion Is Associated with Peroxisome Proliferator-Activated Receptor Isoform Expression in Term Labor.
The American journal of pathology185(7)1981-90(2015 Jul)
He P,Li Y,Ding X,Sun Q,Huang Y,Gu H,Ni X


book

15-PGDH expression as a predictive factor response to neoadjuvant chemotherapy in advanced gastric cancer.
International journal of clinical and experimental pathology8(6)6910-8(2015)
Hu M,Li K,Maskey N,Xu Z,Peng C,Tian S,Li Y,Yang G


book

Expression and Cellular Localization of 15-Hydroxy-Prostaglandin-Dehydrogenase in Abdominal Aortic Aneurysm.
PloS one10(8)e0136201(2015)
Solà-Villà D,Dilmé JF,Rodríguez C,Soto B,Vila L,Escudero JR,Martínez-González J,Camacho M


 
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