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KRT12 cDNA ORF clone, Homo sapiens(Human)

Gene Symbol KRT12
Entrez Gene ID 3859
Full Name keratin 12
Synonyms K12
General protein information
Preferred Names

keratin 12

Names

keratin, type I cytoskeletal 12
CK-12
cytokeratin-12
keratin 12, type I

Gene Type protein-coding
Organism Homo sapiens(Human)
Genome

17

17q21.2

Summary KRT12 encodes the type I intermediate filament chain keratin 12, expressed in corneal epithelia. Mutations in this gene lead to Meesmann corneal dystrophy. [provided by RefSeq, Jul 2008].
Disorder MIM:

601687

Disorder Html: Meesmann corneal dystrophy, 122100 (3)

mRNA and Protein(s)

mRNA Protein Name
NM_000223.3 NP_000214.1 keratin, type I cytoskeletal 12



Macaca mulatta (rhesus monkey) KRT12 XP_001101582.1
Bos taurus (cattle) KRT12 XP_002696056.2
Rattus norvegicus (Norway rat) Krt12 NP_001008761.1
Gallus gallus (chicken) KRT12 XP_004948663.1
Xenopus tropicalis (tropical clawed frog) LOC100486967 XP_002940696.1
Pan troglodytes (chimpanzee) KRT12 XP_001168468.1
Canis lupus familiaris (dog) KRT12 NP_001075890.1
Mus musculus (house mouse) Krt12 NP_034791.2
Homo sapiens (human) KRT12 NP_000214.1


Related articles in PubMed

PAX6 Isoforms, along with Reprogramming Factors, Differentially Regulate the Induction of Cornea-specific Genes.
Sasamoto Y, Hayashi R, Park SJ, Saito-Adachi M, Suzuki Y, Kawasaki S, Quantock AJ, Nakai K, Tsujikawa M, Nishida K
Scientific reports620807(2016 Feb)

siRNA silencing of the mutant keratin 12 allele in corneal limbal epithelial cells grown from patients with Meesmann's epithelial corneal dystrophy.
Courtney DG, Atkinson SD, Allen EH, Moore JE, Walsh CP, Pedrioli DM, MacEwen CJ, Pellegrini G, Maurizi E, Serafini C, Fantacci M, Liao H, Irvine AD, McLean WH, Moore CB
Investigative ophthalmology & visual science55(5)3352-60(2014 May)

KRT12 mutations and in vivo confocal microscopy in two Japanese families with Meesmann corneal dystrophy.
Ogasawara M, Matsumoto Y, Hayashi T, Ohno K, Yamada H, Kawakita T, Dogru M, Shimazaki J, Tsubota K, Tsuneoka H
American journal of ophthalmology157(1)93-102.e1(2014 Jan)

Autosomal-dominant Meesmann epithelial corneal dystrophy without an exon mutation in the keratin-3 or keratin-12 gene in a Chinese family.
Cao W, Yan M, Hao Q, Wang S, Wu L, Liu Q, Li M, Biddle FG, Wu W
The Journal of international medical research41(2)511-8(2013 Apr)

Allele-specific siRNA silencing for the common keratin 12 founder mutation in Meesmann epithelial corneal dystrophy.
Allen EHA, Atkinson SD, Liao H, Moore JE, Pedrioli DML, Smith FJD, McLean WHI, Moore CBT
Investigative ophthalmology & visual science54(1)494-502(2013 Jan)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

we show that the two PAX6 isoforms differentially and cooperatively regulate the expression of genes specific to the structure and functions of the corneal epithelium, particularly keratin 3 (KRT3) and keratin 12 (KRT12). PAX6 isoform-a induced KRT3 expression by targeting its upstream region. KLF4 enhanced this induction. A combination of PAX6 isoform-b, KLF4, and OCT4 induced KRT12 expression
Title: PAX6 Isoforms, along with Reprogramming Factors, Differentially Regulate the Induction of Cornea-specific Genes.

Combined with an effective delivery vehicle this siRNA approach represents a viable treatment option for prevention of the MECD pathology observed in K12-Leu132Pro heterozygous individuals.
Title: siRNA silencing of the mutant keratin 12 allele in corneal limbal epithelial cells grown from patients with Meesmann's epithelial corneal dystrophy.

We identified a novel missense mutation of the KRT12 gene in Meesmann corneal dystrophy. The in vivo confocal microscopy examinations revealed previously unreported depth-dependent ultrastructural changes in the living cornea.
Title: KRT12 mutations and in vivo confocal microscopy in two Japanese families with Meesmann corneal dystrophy.

Exon sequencing of KRT3 and KRT12 in six affected and eight unaffected individuals did not detect any mutations or nucleotide sequence variants in Meesmann epithelial corneal dystrophy.
Title: Autosomal-dominant Meesmann epithelial corneal dystrophy without an exon mutation in the keratin-3 or keratin-12 gene in a Chinese family.

The lead siRNA, with an IC(50) of thirty picomolar, showed no keratin off-target effects or activation of TLR3 in the concentration ranges tested.
Title: Allele-specific siRNA silencing for the common keratin 12 founder mutation in Meesmann epithelial corneal dystrophy.

The following KRT12 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the KRT12 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

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***CloneID RefSeq Accession Definition **Vector *Turnaround time Price (USD) Select
OHu18523 NM_000223.3
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Homo sapiens keratin 12 (KRT12), mRNA. pcDNA3.1+/C-(K)DYK or customized vector
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.


CloneID OHu18523
Clone ID Related Accession (Same CDS sequence) NM_000223.3
Accession Version NM_000223.3 Latest version! Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1485bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2017-04-30
Organism Homo sapiens(Human)
Product keratin, type I cytoskeletal 12
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from D78367.1 and CV573989.1. This sequence is a reference standard in the RefSeqGene project. On Aug 29, 2007 this sequence version replaced NM_000223.2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: D78367.1, AK313747.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1966682, SAMEA1968540 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: full length.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
ATGGATCTCT CCAACAACAC CATGTCACTC TCAGTGCGCA CCCCCGGACT GTCCCGGCGG 
CTCTCCTCGC AGAGTGTGAT AGGCAGACCC AGGGGCATGT CTGCTTCCAG TGTTGGAAGT
GGTTATGGGG GAAGTGCCTT TGGCTTTGGA GCCAGCTGTG GGGGAGGCTT TTCTGCTGCT
TCCATGTTTG GTTCTAGTTC CGGCTTTGGG GGTGGCTCCG GAAGTTCCAT GGCAGGAGGA
CTGGGTGCTG GTTATGGGAG AGCCCTGGGT GGAGGTAGCT TTGGAGGGCT GGGGATGGGA
TTTGGGGGCA GCCCAGGAGG TGGCTCTCTA GGTATTCTCT CGGGCAATGA TGGAGGCCTT
CTTTCTGGAT CAGAAAAAGA AACTATGCAA AATCTTAATG ATAGATTAGC TTCCTACCTG
GATAAGGTGC GAGCTCTAGA AGAGGCTAAT ACTGAGCTAG AAAATAAAAT TCGAGAATGG
TATGAAACAC GAGGAACTGG GACTGCAGAT GCTTCACAGA GCGATTACAG CAAATATTAT
CCACTGATTG AAGACCTCAG GAATAAGATC ATTTCAGCCA GCATTGGAAA TGCCCAGCTC
CTCTTGCAGA TTGACAATGC GAGACTAGCT GCTGAGGACT TCAGGATGAA GTATGAGAAT
GAACTGGCCC TGCGCCAGGG CGTAGAGGCC GACATCAATG GCCTGCGCCG GGTGCTGGAC
GAGCTGACCC TGACCAGGAC CGACCTGGAG ATGCAGATCG AGAGCCTGAA CGAGGAGCTG
GCCTACATGA AGAAGAACCA CGAGGATGAG CTCCAAAGCT TCCGGGTGGG CGGCCCAGGC
GAGGTCAGCG TAGAAATGGA CGCTGCCCCC GGAGTGGACC TCACCAGGCT CCTCAATGAT
ATGCGGGCGC AGTATGAAAC CATCGCTGAG CAGAATCGGA AGGACGCTGA AGCCTGGTTC
ATTGAAAAGA GCGGGGAGCT CCGTAAGGAG ATTAGCACCA ACACCGAGCA GCTTCAGTCC
AGCAAGAGCG AGGTCACCGA CCTGCGTCGC GCCTTTCAGA ACCTGGAGAT CGAGCTACAG
TCCCAGCTCG CCATGAAGAA ATCCCTGGAG GACTCCTTGG CCGAAGCCGA GGGCGATTAC
TGCGCGCAGC TGTCCCAGGT GCAGCAGCTC ATCAGCAACC TGGAGGCACA GCTGCTCCAG
GTGCGCGCGG ACGCAGAGCG CCAGAACGTG GACCACCAGC GGCTGCTGAA TGTCAAGGCC
CGCCTGGAGC TGGAGATTGA GACCTACCGC CGCCTGCTGG ACGGGGAGGC CCAAGGTGAT
GGTTTGGAGG AAAGTTTATT TGTGACAGAC TCCAAATCAC AAGCACAGTC AACTGATTCC
TCTAAAGACC CAACCAAAAC CCGAAAAATC AAGACAGTTG TGCAGGAGAT GGTGAATGGT
GAGGTGGTCT CATCTCAAGT TCAGGAAATT GAAGAACTAA TGTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_000214.1
CDS25..1509
Misc Feature(1)25..396
Misc Feature(2)394..1332
Misc Feature(3)397..1329
Misc Feature(4)397..504
Misc Feature(5)514..570
Misc Feature(6)571..846
Misc Feature(7)847..915
Misc Feature(8)916..1329
Misc Feature(9)1330..1506
Exon (1)1..591
Exon (2)592..674
Exon (3)675..831
Exon (4)832..993
Exon (5)994..1119
Exon (6)1120..1340
Exon (7)1341..1411
Exon (8)1412..1992
Translation

Target ORF information:

RefSeq Version NM_000223.3
Organism Homo sapiens(Human)
Definition Homo sapiens keratin 12 (KRT12), mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_000223.3

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
ATGGATCTCT CCAACAACAC CATGTCACTC TCAGTGCGCA CCCCCGGACT GTCCCGGCGG 
CTCTCCTCGC AGAGTGTGAT AGGCAGACCC AGGGGCATGT CTGCTTCCAG TGTTGGAAGT
GGTTATGGGG GAAGTGCCTT TGGCTTTGGA GCCAGCTGTG GGGGAGGCTT TTCTGCTGCT
TCCATGTTTG GTTCTAGTTC CGGCTTTGGG GGTGGCTCCG GAAGTTCCAT GGCAGGAGGA
CTGGGTGCTG GTTATGGGAG AGCCCTGGGT GGAGGTAGCT TTGGAGGGCT GGGGATGGGA
TTTGGGGGCA GCCCAGGAGG TGGCTCTCTA GGTATTCTCT CGGGCAATGA TGGAGGCCTT
CTTTCTGGAT CAGAAAAAGA AACTATGCAA AATCTTAATG ATAGATTAGC TTCCTACCTG
GATAAGGTGC GAGCTCTAGA AGAGGCTAAT ACTGAGCTAG AAAATAAAAT TCGAGAATGG
TATGAAACAC GAGGAACTGG GACTGCAGAT GCTTCACAGA GCGATTACAG CAAATATTAT
CCACTGATTG AAGACCTCAG GAATAAGATC ATTTCAGCCA GCATTGGAAA TGCCCAGCTC
CTCTTGCAGA TTGACAATGC GAGACTAGCT GCTGAGGACT TCAGGATGAA GTATGAGAAT
GAACTGGCCC TGCGCCAGGG CGTAGAGGCC GACATCAATG GCCTGCGCCG GGTGCTGGAC
GAGCTGACCC TGACCAGGAC CGACCTGGAG ATGCAGATCG AGAGCCTGAA CGAGGAGCTG
GCCTACATGA AGAAGAACCA CGAGGATGAG CTCCAAAGCT TCCGGGTGGG CGGCCCAGGC
GAGGTCAGCG TAGAAATGGA CGCTGCCCCC GGAGTGGACC TCACCAGGCT CCTCAATGAT
ATGCGGGCGC AGTATGAAAC CATCGCTGAG CAGAATCGGA AGGACGCTGA AGCCTGGTTC
ATTGAAAAGA GCGGGGAGCT CCGTAAGGAG ATTAGCACCA ACACCGAGCA GCTTCAGTCC
AGCAAGAGCG AGGTCACCGA CCTGCGTCGC GCCTTTCAGA ACCTGGAGAT CGAGCTACAG
TCCCAGCTCG CCATGAAGAA ATCCCTGGAG GACTCCTTGG CCGAAGCCGA GGGCGATTAC
TGCGCGCAGC TGTCCCAGGT GCAGCAGCTC ATCAGCAACC TGGAGGCACA GCTGCTCCAG
GTGCGCGCGG ACGCAGAGCG CCAGAACGTG GACCACCAGC GGCTGCTGAA TGTCAAGGCC
CGCCTGGAGC TGGAGATTGA GACCTACCGC CGCCTGCTGG ACGGGGAGGC CCAAGGTGAT
GGTTTGGAGG AAAGTTTATT TGTGACAGAC TCCAAATCAC AAGCACAGTC AACTGATTCC
TCTAAAGACC CAACCAAAAC CCGAAAAATC AAGACAGTTG TGCAGGAGAT GGTGAATGGT
GAGGTGGTCT CATCTCAAGT TCAGGAAATT GAAGAACTAA TGTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

book

PAX6 Isoforms, along with Reprogramming Factors, Differentially Regulate the Induction of Cornea-specific Genes.
Scientific reports620807(2016 Feb)
Sasamoto Y,Hayashi R,Park SJ,Saito-Adachi M,Suzuki Y,Kawasaki S,Quantock AJ,Nakai K,Tsujikawa M,Nishida K


book

siRNA silencing of the mutant keratin 12 allele in corneal limbal epithelial cells grown from patients with Meesmann's epithelial corneal dystrophy.
Investigative ophthalmology & visual science55(5)3352-60(2014 May)
Courtney DG,Atkinson SD,Allen EH,Moore JE,Walsh CP,Pedrioli DM,MacEwen CJ,Pellegrini G,Maurizi E,Serafini C,Fantacci M,Liao H,Irvine AD,McLean WH,Moore CB


book

KRT12 mutations and in vivo confocal microscopy in two Japanese families with Meesmann corneal dystrophy.
American journal of ophthalmology157(1)93-102.e1(2014 Jan)
Ogasawara M,Matsumoto Y,Hayashi T,Ohno K,Yamada H,Kawakita T,Dogru M,Shimazaki J,Tsubota K,Tsuneoka H


book

Autosomal-dominant Meesmann epithelial corneal dystrophy without an exon mutation in the keratin-3 or keratin-12 gene in a Chinese family.
The Journal of international medical research41(2)511-8(2013 Apr)
Cao W,Yan M,Hao Q,Wang S,Wu L,Liu Q,Li M,Biddle FG,Wu W


book

Allele-specific siRNA silencing for the common keratin 12 founder mutation in Meesmann epithelial corneal dystrophy.
Investigative ophthalmology & visual science54(1)494-502(2013 Jan)
Allen EHA,Atkinson SD,Liao H,Moore JE,Pedrioli DML,Smith FJD,McLean WHI,Moore CBT


 
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