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SNU13 cDNA ORF clone, Homo sapiens(human)

Summary

Gene Symbol SNU13
Entrez Gene ID 4809
Full Name small nuclear ribonucleoprotein 13
Synonyms 15.5K,FA-1,FA1,NHP2L1,NHPX,OTK27,SNRNP15-5,SPAG12,SSFA1
General protein information
Preferred Names

small nuclear ribonucleoprotein 13

Names

NHP2-like protein 1
NHP2 non-histone chromosome protein 2-like 1
SNU13 homolog, small nuclear ribonucleoprotein (U4/U6.U5)
U4/U6.U5 tri-snRNP 15.5 kDa protein
[U4/U6.U5] tri-snRNP 15.5 kD RNA binding protein
high mobility group-like nuclear protein 2 homolog 1
non-histone chromosome protein 2-like 1
sperm specific antigen 1
Gene Type protein-coding
Organism Homo sapiens(human)
Genome

22

22q13.2
Summary Originally named because of its sequence similarity to the Saccharomyces cerevisiae NHP2 (non-histone protein 2), this protein appears to be a highly conserved nuclear protein that is a component of the [U4/U6.U5] tri-snRNP. It binds to the 5' stem-loop of U4 snRNA. Two transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Jul 2008].
Disorder MIM:

601304


Reference Sequences (RefSeq)

mRNA and Protein(s)

mRNA Protein Name
NM_005008.4 NP_004999.1 NHP2-like protein 1
NM_001003796.2 NP_001003796.1 NHP2-like protein 1
NM_005008.3 NP_004999.1 NHP2-like protein 1
XM_017028811.1 XP_016884300.1 NHP2-like protein 1 isoform X1
NM_001003796.1 NP_001003796.1 NHP2-like protein 1

Pathways from BioSystems


Homologies


Pan troglodytes (chimpanzee) NHP2L1 XP_003317315.1
Canis lupus familiaris (dog) NHP2L1 XP_003431541.1
Rattus norvegicus (Norway rat) Nhp2l1 NP_997680.1
Caenorhabditis elegans (roundworm) M28.5 NP_496300.1
Neurospora crassa NCU01331 XP_961417.1
Bos taurus (cattle) NHP2L1 NP_001070472.1
Mus musculus (house mouse) Nhp2l1 NP_035612.2
Magnaporthe oryzae (rice blast fungus) MGG_02467 XP_003709261.1
Arabidopsis thaliana (thale cress) AT4G12600 NP_192997.1
Homo sapiens (human) NHP2L1 NP_001003796.1
LOC708283 XP_001105963.1
Danio rerio (zebrafish) nhp2l1a NP_956606.1
Kluyveromyces lactis KLLA0E22529g XP_454971.1
Schizosaccharomyces pombe (fission yeast) snu13 NP_593592.1
Arabidopsis thaliana (thale cress) AT4G22380 NP_193969.1
Arabidopsis thaliana (thale cress) AT5G20160 NP_850856.1
Eremothecium gossypii AGOS_AEL070W NP_984791.1
Rattus norvegicus (Norway rat) LOC100359574 XP_003749200.1
Gallus gallus (chicken) NHP2L1 XP_416225.3
Danio rerio (zebrafish) nhp2l1b NP_955829.1
Drosophila melanogaster (fruit fly) hoip NP_001260293.1
Anopheles gambiae (African malaria mosquito) AgaP_AGAP008163 XP_317299.3
Saccharomyces cerevisiae (baker's yeast) SNU13 NP_010888.1
Oryza sativa (rice) Os03g0241200 NP_001049514.1
Xenopus tropicalis (tropical clawed frog) nhp2l1 NP_988994.1

Gene Ontology


Bibliography

Related articles in PubMed

Cryo-EM Structure of a Pre-catalytic Human Spliceosome Primed for Activation.
Bertram K, Agafonov DE, Dybkov O, Haselbach D, Leelaram MN, Will CL, Urlaub H, Kastner B, L?hrmann R, Stark H
Cell170(4)701-713.e11(2017 Aug)

Structural Features of the Box C/D snoRNP Pre-assembly Process Are Conserved through Species.
Quinternet M, Chagot ME, Roth? B, Tiotiu D, Charpentier B, Manival X
Structure (London, England : 1993)24(10)1693-1706(2016 Oct)

Molecular architecture of the human U4/U6.U5 tri-snRNP.
Agafonov DE, Kastner B, Dybkov O, Hofele RV, Liu WT, Urlaub H, L?hrmann R, Stark H
Science (New York, N.Y.)351(6280)1416-20(2016 Mar)

Insights into RNA biology from an atlas of mammalian mRNA-binding proteins.
Castello A, Fischer B, Eichelbaum K, Horos R, Beckmann BM, Strein C, Davey NE, Humphreys DT, Preiss T, Steinmetz LM, Krijgsveld J, Hentze MW
Cell149(6)1393-406(2012 Jun)

The mRNA-bound proteome and its global occupancy profile on protein-coding transcripts.
Baltz AG, Munschauer M, Schwanh?usser B, Vasile A, Murakawa Y, Schueler M, Youngs N, Penfold-Brown D, Drew K, Milek M, Wyler E, Bonneau R, Selbach M, Dieterich C, Landthaler M
Molecular cell46(5)674-90(2012 Jun)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Results solve the structure of a complex resulting from interaction between protein fragments of human NUFIP1 and its cofactor ZNHIT3, and emphasize their imbrication. Also, it seems that the complexes involving NUFIP1, ZNHIT3, and SNU13 share strong structural similarities between human and yeast, suggesting that the initial steps of the box C/D snoRNP assembly process are conserved among species.
Title: Structural Features of the Box C/D snoRNP Pre-assembly Process Are Conserved through Species.

snoRNP assembly factor NUFIP can regulate the interactions between TIP48 and TIP49 and the core box C/D proteins.
Title: Evidence that the AAA+ proteins TIP48 and TIP49 bridge interactions between 15.5K and the related NOP56 and NOP58 proteins during box C/D snoRNP biogenesis.

describe the solution NMR structure of free 15.5K, as well as studies of conformational flexibility from 15N NMR relaxation and H/D exchange experiments
Title: Functional implications for a prototypical K-turn binding protein from structural and dynamical studies of 15.5K.

Data show that binding of the 15.5 kD RNA binding protein to the box C/D motif of snoRNAs is essential for the association of other snoRNP-associated proteins.
Title: Conserved stem II of the box C/D motif is essential for nucleolar localization and is required, along with the 15.5K protein, for the hierarchical assembly of the box C/D snoRNP.

the testis-specific expression of FA-1 antigen at the mRNA level
Title: Molecular cloning and sequencing of cDNA encoding for human FA-1 antigen.

The following SNU13 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the SNU13 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OHu03482 NM_005008.4
Latest version!		 Homo sapiens small nuclear ribonucleoprotein 13 (SNU13), transcript variant 1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK		 $49.50-$69.30
$99.00
OHu03482 NM_001003796.2
Latest version!		 Homo sapiens small nuclear ribonucleoprotein 13 (SNU13), transcript variant 2, mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK		 $49.50-$69.30
$99.00
OHu93504 XM_017028811.1
Latest version!		 Homo sapiens small nuclear ribonucleoprotein 13 (SNU13), transcript variant X1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 9-11 $69.30
$99.00
View Old Accession Versions >>
OHu03482 NM_005008.3 Homo sapiens small nuclear ribonucleoprotein 13 (SNU13), transcript variant 1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK		 $49.50-$69.30
$99.00
OHu03482 NM_001003796.1 Homo sapiens small nuclear ribonucleoprotein 13 (SNU13), transcript variant 2, mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK		 $49.50-$69.30
$99.00
Hide Old Accession Versions >>

ORF Online Only Promotion

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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)


CloneID OHu03482
Clone ID Related Accession (Same CDS sequence) NM_005008.4 , NM_001003796.2 , NM_005008.3 , NM_001003796.1
Accession Version NM_005008.4 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 387bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2019-09-09
Organism Homo sapiens(human)
Product NHP2-like protein 1
Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DB021462.1, D50420.1 and BC019282.2. This sequence is a reference standard in the RefSeqGene project. On May 31, 2019 this sequence version replaced NM_005008.3. Transcript Variant: This variant (1) represents the longer transcript. Variants 1 and 2 encode the same protein. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660809.60148.1, DRR138506.8620.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968540, SAMEA2161674 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end. 

1
61
121
181
241
301
361
ATGACTGAGG CTGATGTGAA TCCAAAGGCC TATCCCCTTG CCGATGCCCA CCTCACCAAG 
AAGCTACTGG ACCTCGTTCA GCAGTCATGT AACTATAAGC AGCTTCGGAA AGGAGCCAAT 
GAGGCCACCA AAACCCTCAA CAGGGGCATC TCTGAGTTCA TCGTGATGGC TGCAGACGCC 
GAGCCACTGG AGATCATTCT GCACCTGCCG CTGCTGTGTG AAGACAAGAA TGTGCCCTAC 
GTGTTTGTGC GCTCCAAGCA GGCCCTGGGG AGAGCCTGTG GGGTCTCCAG GCCTGTCATC 
GCCTGTTCTG TCACCATCAA AGAAGGCTCG CAGCTGAAAC AGCAGATCCA ATCCATTCAG 
CAGTCCATTG AAAGGCTCTT AGTCTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_004999.1
CDS304..690
Translation

Target ORF information:

RefSeq Version NM_005008.4
Organism Homo sapiens(human)
Definition Homo sapiens small nuclear ribonucleoprotein 13 (SNU13), transcript variant 1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 NM_005008.4

ORF Insert Sequence:

1
61
121
181
241
301
361
ATGACTGAGG CTGATGTGAA TCCAAAGGCC TATCCCCTTG CCGATGCCCA CCTCACCAAG 
AAGCTACTGG ACCTCGTTCA GCAGTCATGT AACTATAAGC AGCTTCGGAA AGGAGCCAAT 
GAGGCCACCA AAACCCTCAA CAGGGGCATC TCTGAGTTCA TCGTGATGGC TGCAGACGCC 
GAGCCACTGG AGATCATTCT GCACCTGCCG CTGCTGTGTG AAGACAAGAA TGTGCCCTAC 
GTGTTTGTGC GCTCCAAGCA GGCCCTGGGG AGAGCCTGTG GGGTCTCCAG GCCTGTCATC 
GCCTGTTCTG TCACCATCAA AGAAGGCTCG CAGCTGAAAC AGCAGATCCA ATCCATTCAG 
CAGTCCATTG AAAGGCTCTT AGTCTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu03482
Clone ID Related Accession (Same CDS sequence) NM_005008.4 , NM_001003796.2 , NM_005008.3 , NM_001003796.1
Accession Version NM_001003796.2 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 387bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2019-09-09
Organism Homo sapiens(human)
Product NHP2-like protein 1
Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from CF552526.1, D50420.1 and BI770951.1. This sequence is a reference standard in the RefSeqGene project. On Nov 22, 2018 this sequence version replaced NM_001003796.1. Transcript Variant: This variant (2) differs in the 5' UTR compared to variant 1. Variants 1 and 2 use different start codons but encode the same protein. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR3476690.67582.1, SRR5189661.62648.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968540, SAMEA2142348 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000401959.6/ ENSP00000383949.1 RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: full length. 

1
61
121
181
241
301
361
ATGACTGAGG CTGATGTGAA TCCAAAGGCC TATCCCCTTG CCGATGCCCA CCTCACCAAG 
AAGCTACTGG ACCTCGTTCA GCAGTCATGT AACTATAAGC AGCTTCGGAA AGGAGCCAAT 
GAGGCCACCA AAACCCTCAA CAGGGGCATC TCTGAGTTCA TCGTGATGGC TGCAGACGCC 
GAGCCACTGG AGATCATTCT GCACCTGCCG CTGCTGTGTG AAGACAAGAA TGTGCCCTAC 
GTGTTTGTGC GCTCCAAGCA GGCCCTGGGG AGAGCCTGTG GGGTCTCCAG GCCTGTCATC 
GCCTGTTCTG TCACCATCAA AGAAGGCTCG CAGCTGAAAC AGCAGATCCA ATCCATTCAG 
CAGTCCATTG AAAGGCTCTT AGTCTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001003796.1
CDS72..458
Translation

Target ORF information:

RefSeq Version NM_001003796.2
Organism Homo sapiens(human)
Definition Homo sapiens small nuclear ribonucleoprotein 13 (SNU13), transcript variant 2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 NM_001003796.2

ORF Insert Sequence:

1
61
121
181
241
301
361
ATGACTGAGG CTGATGTGAA TCCAAAGGCC TATCCCCTTG CCGATGCCCA CCTCACCAAG 
AAGCTACTGG ACCTCGTTCA GCAGTCATGT AACTATAAGC AGCTTCGGAA AGGAGCCAAT 
GAGGCCACCA AAACCCTCAA CAGGGGCATC TCTGAGTTCA TCGTGATGGC TGCAGACGCC 
GAGCCACTGG AGATCATTCT GCACCTGCCG CTGCTGTGTG AAGACAAGAA TGTGCCCTAC 
GTGTTTGTGC GCTCCAAGCA GGCCCTGGGG AGAGCCTGTG GGGTCTCCAG GCCTGTCATC 
GCCTGTTCTG TCACCATCAA AGAAGGCTCG CAGCTGAAAC AGCAGATCCA ATCCATTCAG 
CAGTCCATTG AAAGGCTCTT AGTCTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu03482
Clone ID Related Accession (Same CDS sequence) NM_005008.4 , NM_001003796.2 , NM_005008.3 , NM_001003796.1
Accession Version NM_005008.3 Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 387bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2019-02-22
Organism Homo sapiens(human)
Product NHP2-like protein 1
Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from CF552526.1, DB021462.1, D50420.1 and BC019282.2. This sequence is a reference standard in the RefSeqGene project. On Jun 2, 2012 this sequence version replaced NM_005008.2. Transcript Variant: This variant (1) represents the longer transcript. Variants 1 and 2 encode the same protein. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660809.60148.1, SRR1803615.179966.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968540, SAMEA2161674 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end. 

1
61
121
181
241
301
361
ATGACTGAGG CTGATGTGAA TCCAAAGGCC TATCCCCTTG CCGATGCCCA CCTCACCAAG 
AAGCTACTGG ACCTCGTTCA GCAGTCATGT AACTATAAGC AGCTTCGGAA AGGAGCCAAT 
GAGGCCACCA AAACCCTCAA CAGGGGCATC TCTGAGTTCA TCGTGATGGC TGCAGACGCC 
GAGCCACTGG AGATCATTCT GCACCTGCCG CTGCTGTGTG AAGACAAGAA TGTGCCCTAC 
GTGTTTGTGC GCTCCAAGCA GGCCCTGGGG AGAGCCTGTG GGGTCTCCAG GCCTGTCATC 
GCCTGTTCTG TCACCATCAA AGAAGGCTCG CAGCTGAAAC AGCAGATCCA ATCCATTCAG 
CAGTCCATTG AAAGGCTCTT AGTCTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_004999.1
CDS346..732
Translation

Target ORF information:

RefSeq Version NM_005008.3
Organism Homo sapiens(human)
Definition Homo sapiens small nuclear ribonucleoprotein 13 (SNU13), transcript variant 1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 NM_005008.3

ORF Insert Sequence:

1
61
121
181
241
301
361
ATGACTGAGG CTGATGTGAA TCCAAAGGCC TATCCCCTTG CCGATGCCCA CCTCACCAAG 
AAGCTACTGG ACCTCGTTCA GCAGTCATGT AACTATAAGC AGCTTCGGAA AGGAGCCAAT 
GAGGCCACCA AAACCCTCAA CAGGGGCATC TCTGAGTTCA TCGTGATGGC TGCAGACGCC 
GAGCCACTGG AGATCATTCT GCACCTGCCG CTGCTGTGTG AAGACAAGAA TGTGCCCTAC 
GTGTTTGTGC GCTCCAAGCA GGCCCTGGGG AGAGCCTGTG GGGTCTCCAG GCCTGTCATC 
GCCTGTTCTG TCACCATCAA AGAAGGCTCG CAGCTGAAAC AGCAGATCCA ATCCATTCAG 
CAGTCCATTG AAAGGCTCTT AGTCTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu03482
Clone ID Related Accession (Same CDS sequence) NM_005008.4 , NM_001003796.2 , NM_005008.3 , NM_001003796.1
Accession Version NM_001003796.1 Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 387bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2017-12-26
Organism Homo sapiens(human)
Product NHP2-like protein 1
Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from CF552526.1, D50420.1 and BI770951.1. This sequence is a reference standard in the RefSeqGene project. Transcript Variant: This variant (2) differs in the 5' UTR compared to variant 1. Variants 1 and 2 use different start codons but encode the same protein. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1163655.302363.1, SRR1163657.316603.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968540, SAMEA2142348 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end. 

1
61
121
181
241
301
361
ATGACTGAGG CTGATGTGAA TCCAAAGGCC TATCCCCTTG CCGATGCCCA CCTCACCAAG 
AAGCTACTGG ACCTCGTTCA GCAGTCATGT AACTATAAGC AGCTTCGGAA AGGAGCCAAT 
GAGGCCACCA AAACCCTCAA CAGGGGCATC TCTGAGTTCA TCGTGATGGC TGCAGACGCC 
GAGCCACTGG AGATCATTCT GCACCTGCCG CTGCTGTGTG AAGACAAGAA TGTGCCCTAC 
GTGTTTGTGC GCTCCAAGCA GGCCCTGGGG AGAGCCTGTG GGGTCTCCAG GCCTGTCATC 
GCCTGTTCTG TCACCATCAA AGAAGGCTCG CAGCTGAAAC AGCAGATCCA ATCCATTCAG 
CAGTCCATTG AAAGGCTCTT AGTCTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001003796.1
CDS114..500
Translation

Target ORF information:

RefSeq Version NM_001003796.1
Organism Homo sapiens(human)
Definition Homo sapiens small nuclear ribonucleoprotein 13 (SNU13), transcript variant 2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 NM_001003796.1

ORF Insert Sequence:

1
61
121
181
241
301
361
ATGACTGAGG CTGATGTGAA TCCAAAGGCC TATCCCCTTG CCGATGCCCA CCTCACCAAG 
AAGCTACTGG ACCTCGTTCA GCAGTCATGT AACTATAAGC AGCTTCGGAA AGGAGCCAAT 
GAGGCCACCA AAACCCTCAA CAGGGGCATC TCTGAGTTCA TCGTGATGGC TGCAGACGCC 
GAGCCACTGG AGATCATTCT GCACCTGCCG CTGCTGTGTG AAGACAAGAA TGTGCCCTAC 
GTGTTTGTGC GCTCCAAGCA GGCCCTGGGG AGAGCCTGTG GGGTCTCCAG GCCTGTCATC 
GCCTGTTCTG TCACCATCAA AGAAGGCTCG CAGCTGAAAC AGCAGATCCA ATCCATTCAG 
CAGTCCATTG AAAGGCTCTT AGTCTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu93504
Clone ID Related Accession (Same CDS sequence) XM_017028811.1
Accession Version XM_017028811.1 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 303bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2019-12-05
Organism Homo sapiens(human)
Product NHP2-like protein 1 isoform X1
Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000022.11) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

1
61
121
181
241
301
ATGCTCTTGG TGCAGGGCCA GTTTCCGCGC CAGTGCCCCC GTAGAGGGCG GGGCCCACTC 
GCTCGCGTAG CGTCACCTTC GCTTCCTGCC GCGCGGGGCC ATTTCCGCTG CTGCTTCTGT 
GAGTTTTTCC GGTGCACGCG AGTGCTTCTG AAACGTCAGC TGCGCTCCCC TAGGAGTGCT 
GAGCCCGCGG AACCGCAGCC ATGGAGGAGA TCTCCCATGG GTCTGCGCTT AGTGCCCGCC 
GCGAGTCACA CGCGCCAGGG ACCTAGAGGA GCCTTTGGCA TTTGGGGGCT GTGGGGAACA 
TGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_016884300.1
CDS44..346
Translation

Target ORF information:

RefSeq Version XM_017028811.1
Organism Homo sapiens(human)
Definition Homo sapiens small nuclear ribonucleoprotein 13 (SNU13), transcript variant X1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 XM_017028811.1

ORF Insert Sequence:

1
61
121
181
241
301
ATGCTCTTGG TGCAGGGCCA GTTTCCGCGC CAGTGCCCCC GTAGAGGGCG GGGCCCACTC 
GCTCGCGTAG CGTCACCTTC GCTTCCTGCC GCGCGGGGCC ATTTCCGCTG CTGCTTCTGT 
GAGTTTTTCC GGTGCACGCG AGTGCTTCTG AAACGTCAGC TGCGCTCCCC TAGGAGTGCT 
GAGCCCGCGG AACCGCAGCC ATGGAGGAGA TCTCCCATGG GTCTGCGCTT AGTGCCCGCC 
GCGAGTCACA CGCGCCAGGG ACCTAGAGGA GCCTTTGGCA TTTGGGGGCT GTGGGGAACA 
TGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

PubMed

book

Cryo-EM Structure of a Pre-catalytic Human Spliceosome Primed for Activation.
Cell170(4)701-713.e11(2017 Aug)
Bertram K,Agafonov DE,Dybkov O,Haselbach D,Leelaram MN,Will CL,Urlaub H,Kastner B,L?hrmann R,Stark H


book

Structural Features of the Box C/D snoRNP Pre-assembly Process Are Conserved through Species.
Structure (London, England : 1993)24(10)1693-1706(2016 Oct)
Quinternet M,Chagot ME,Roth? B,Tiotiu D,Charpentier B,Manival X


book

Molecular architecture of the human U4/U6.U5 tri-snRNP.
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Agafonov DE,Kastner B,Dybkov O,Hofele RV,Liu WT,Urlaub H,L?hrmann R,Stark H


book

Insights into RNA biology from an atlas of mammalian mRNA-binding proteins.
Cell149(6)1393-406(2012 Jun)
Castello A,Fischer B,Eichelbaum K,Horos R,Beckmann BM,Strein C,Davey NE,Humphreys DT,Preiss T,Steinmetz LM,Krijgsveld J,Hentze MW


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The mRNA-bound proteome and its global occupancy profile on protein-coding transcripts.
Molecular cell46(5)674-90(2012 Jun)
Baltz AG,Munschauer M,Schwanh?usser B,Vasile A,Murakawa Y,Schueler M,Youngs N,Penfold-Brown D,Drew K,Milek M,Wyler E,Bonneau R,Selbach M,Dieterich C,Landthaler M