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SHISA5 cDNA ORF clone, Homo sapiens(human)

Summary

Gene Symbol SHISA5
Entrez Gene ID 51246
Full Name shisa family member 5
Synonyms SCOTIN
General protein information
Preferred Names

shisa family member 5

Names

protein shisa-5
putative NF-kappa-B-activating protein 120
shisa homolog 5
Gene Type protein-coding
Organism Homo sapiens(human)
Genome

3

3p21.31
Summary This gene encodes a member of the shisa family. The encoded protein is localized to the endoplasmic reticulum, and together with p53 induces apoptosis in a caspase-dependent manner. Alternative splicing results in multiple transcript variants. Related pseudogenes of this gene are found on chromosome X. [provided by RefSeq, Apr 2016].
Disorder MIM:

607290


Reference Sequences (RefSeq)

mRNA and Protein(s)

mRNA Protein Name
NM_001272067.1 NP_001258996.1 protein shisa-5 isoform c
NM_001272082.2 NP_001259011.1 protein shisa-5 isoform d
NM_016479.4 NP_057563.3 protein shisa-5 isoform a precursor
NM_001272066.1 NP_001258995.1 protein shisa-5 isoform c
XM_011533796.1 XP_011532098.1 protein shisa-5 isoform X1
NM_001272065.1 NP_001258994.1 protein shisa-5 isoform b precursor
XM_006713188.3 XP_006713251.1 protein shisa-5 isoform X1
NM_001272082.1 NP_001259011.1 protein shisa-5 isoform d
XM_005265205.1 XP_005265262.1 protein shisa-5 isoform X1
NM_001272083.1 NP_001259012.1 protein shisa-5 isoform e
XM_006713188.2 XP_006713251.1 protein shisa-5 isoform X1
NM_001272068.1 NP_001258997.1 protein shisa-5 isoform c
NM_001272083.2 NP_001259012.1 protein shisa-5 isoform e

Pathways from BioSystems


Homologies


Homo sapiens (human) SHISA5 NP_057563.3
Bos taurus (cattle) SHISA5 NP_001030563.1
Mus musculus (house mouse) Shisa5 NP_001271261.1
Rattus norvegicus (Norway rat) Shisa5 NP_001006990.1
Pan troglodytes (chimpanzee) SHISA5 NP_001233356.1
LOC709615 XP_001098155.1

Gene Ontology


Bibliography

Related articles in PubMed

Interferon-inducible protein SCOTIN interferes with HCV replication through the autolysosomal degradation of NS5A.
Kim N, Kim MJ, Sung PS, Bae YC, Shin EC, Yoo JY
Nature communications710631(2016 Feb)

Identification of neuroglycan C and interacting partners as potential susceptibility genes for schizophrenia in a Southern Chinese population.
So HC, Fong PY, Chen RY, Hui TC, Ng MY, Cherny SS, Mak WW, Cheung EF, Chan RC, Chen EY, Li T, Sham PC
American journal of medical genetics. Part B, Neuropsychiatric genetics : the official publication of the International Society of Psychiatric Genetics153B(1)103-13(2010 Jan)

The calcium binding protein ALG-2 binds and stabilizes Scotin, a p53-inducible gene product localized at the endoplasmic reticulum membrane.
Draeby I, Woods YL, la Cour JM, Mollerup J, Bourdon JC, Berchtold MW
Archives of biochemistry and biophysics467(1)87-94(2007 Nov)

WWOX binds the specific proline-rich ligand PPXY: identification of candidate interacting proteins.
Ludes-Meyers JH, Kil H, Bednarek AK, Drake J, Bedford MT, Aldaz CM
Oncogene23(29)5049-55(2004 Jun)

Large-scale identification and characterization of human genes that activate NF-kappaB and MAPK signaling pathways.
Matsuda A, Suzuki Y, Honda G, Muramatsu S, Matsuzaki O, Nagano Y, Doi T, Shimotohno K, Harada T, Nishida E, Hayashi H, Sugano S
Oncogene22(21)3307-18(2003 May)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

IFN-beta-induced SCOTIN recruits the HCV NS5A protein to autophagosomes for degradation, thereby restricting HCV replication.
Title: Interferon-inducible protein SCOTIN interferes with HCV replication through the autolysosomal degradation of NS5A.

Observational study of gene-disease association and gene-gene interaction. (HuGE Navigator)
Title: Identification of neuroglycan C and interacting partners as potential susceptibility genes for schizophrenia in a Southern Chinese population.

ALG-2 binding to Scotin is strictly calcium dependent, indicating a role of this interaction in calcium signaling pathways
Title: The calcium binding protein ALG-2 binds and stabilizes Scotin, a p53-inducible gene product localized at the endoplasmic reticulum membrane.

Scotin plays a role in p53-dependent apoptosis
Title: Scotin, a novel p53-inducible proapoptotic protein located in the ER and the nuclear membrane.

The following SHISA5 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the SHISA5 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OHu04072 NM_001272082.2
Latest version!		 Homo sapiens shisa family member 5 (SHISA5), transcript variant 6, mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK		 $49.50-$69.30
$99.00
OHu07269 NM_016479.4
Latest version!		 Homo sapiens shisa family member 5 (SHISA5), transcript variant 1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK		 $49.50-$69.30
$99.00
OHu04069 NM_001272065.1
Latest version!		 Homo sapiens shisa family member 5 (SHISA5), transcript variant 2, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $139.30
$199.00
OHu04070 NM_001272066.1
Latest version!		 Homo sapiens shisa family member 5 (SHISA5), transcript variant 3, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $139.30
$199.00
OHu04070 NM_001272068.1
Latest version!		 Homo sapiens shisa family member 5 (SHISA5), transcript variant 5, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $139.30
$199.00
OHu04070 NM_001272067.1
Latest version!		 Homo sapiens shisa family member 5 (SHISA5), transcript variant 4, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $139.30
$199.00
OHu04073 NM_001272083.2
Latest version!		 Homo sapiens shisa family member 5 (SHISA5), transcript variant 7, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $69.30
$99.00
OHu04070 XM_011533796.1
Latest version!		 Homo sapiens shisa family member 5 (SHISA5), transcript variant X1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $139.30
$199.00
OHu04070 XM_006713188.3
Latest version!		 Homo sapiens shisa family member 5 (SHISA5), transcript variant X3, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $139.30
$199.00
OHu04070 XM_005265205.1
Latest version!		 Homo sapiens shisa family member 5 (SHISA5), transcript variant X2, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $139.30
$199.00
View Old Accession Versions >>
OHu04072 NM_001272082.1 Homo sapiens shisa family member 5 (SHISA5), transcript variant 6, mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK		 $49.50-$69.30
$99.00
OHu04070 XM_006713188.2 Homo sapiens shisa family member 5 (SHISA5), transcript variant X3, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $139.30
$199.00
OHu04073 NM_001272083.1 Homo sapiens shisa family member 5 (SHISA5), transcript variant 7, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $69.30
$99.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)


CloneID OHu04072
Clone ID Related Accession (Same CDS sequence) NM_001272082.2 , NM_001272082.1
Accession Version NM_001272082.2 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 414bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2019-06-01
Organism Homo sapiens(human)
Product protein shisa-5 isoform d
Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA046900.1, BC001463.2, AL832568.1, AI718508.1 and AF520698.1. On Jun 2, 2019 this sequence version replaced NM_001272082.1. Transcript Variant: This variant (6) contains an alternate 5' terminal exon, lacks a portion of the 5' coding region, and initiates translation at an alternate start codon, compared to variant 1. The encoded isoform (d) has a distinct N-terminus and is shorter than isoform a. ##Evidence-Data-START## Transcript exon combination :: AL832568.1, BC020279.2 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2147596, SAMEA2149178 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end. 

1
61
121
181
241
301
361
ATGGGGTTCG GAGCGACCTT GGCCGTTGGC CTGACCATCT TTGTGCTGTC TGTCGTCACT 
ATCATCATCT GCTTCACCTG CTCCTGCTGC TGCCTTTACA AGACGTGCCG CCGACCACGT 
CCGGTTGTCA CCACCACCAC ATCCACCACT GTGGTGCATG CCCCTTATCC TCAGCCTCCA 
AGTGTGCCGC CCAGCTACCC TGGACCAAGC TACCAGGGCT ACCACACCAT GCCGCCTCAG 
CCAGGGATGC CAGCAGCACC CTACCCAATG CAGTACCCAC CACCTTACCC AGCCCAGCCC 
ATGGGCCCAC CGGCCTACCA CGAGACCCTG GCTGGAGGAG CAGCCGCGCC CTACCCCGCC 
AGCCAGCCTC CTTACAACCC GGCCTACATG GATGCCCCGA AGGCGGCCCT CTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001259011.1
CDS145..558
Translation

Target ORF information:

RefSeq Version NM_001272082.2
Organism Homo sapiens(human)
Definition Homo sapiens shisa family member 5 (SHISA5), transcript variant 6, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 NM_001272082.2

ORF Insert Sequence:

1
61
121
181
241
301
361
ATGGGGTTCG GAGCGACCTT GGCCGTTGGC CTGACCATCT TTGTGCTGTC TGTCGTCACT 
ATCATCATCT GCTTCACCTG CTCCTGCTGC TGCCTTTACA AGACGTGCCG CCGACCACGT 
CCGGTTGTCA CCACCACCAC ATCCACCACT GTGGTGCATG CCCCTTATCC TCAGCCTCCA 
AGTGTGCCGC CCAGCTACCC TGGACCAAGC TACCAGGGCT ACCACACCAT GCCGCCTCAG 
CCAGGGATGC CAGCAGCACC CTACCCAATG CAGTACCCAC CACCTTACCC AGCCCAGCCC 
ATGGGCCCAC CGGCCTACCA CGAGACCCTG GCTGGAGGAG CAGCCGCGCC CTACCCCGCC 
AGCCAGCCTC CTTACAACCC GGCCTACATG GATGCCCCGA AGGCGGCCCT CTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu04072
Clone ID Related Accession (Same CDS sequence) NM_001272082.2 , NM_001272082.1
Accession Version NM_001272082.1 Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 414bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2018-06-24
Organism Homo sapiens(human)
Product protein shisa-5 isoform d
Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA046900.1, BC001463.2, AL832568.1, AI718508.1 and AF520698.1. Transcript Variant: This variant (6) contains an alternate 5' terminal exon, lacks a portion of the 5' coding region, and initiates translation at an alternate start codon, compared to variant 1. The encoded isoform (d) has a distinct N-terminus and is shorter than isoform a. ##Evidence-Data-START## Transcript exon combination :: DA046900.1, SRR1803616.163312.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2147596, SAMEA2149178 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end. 

1
61
121
181
241
301
361
ATGGGGTTCG GAGCGACCTT GGCCGTTGGC CTGACCATCT TTGTGCTGTC TGTCGTCACT 
ATCATCATCT GCTTCACCTG CTCCTGCTGC TGCCTTTACA AGACGTGCCG CCGACCACGT 
CCGGTTGTCA CCACCACCAC ATCCACCACT GTGGTGCATG CCCCTTATCC TCAGCCTCCA 
AGTGTGCCGC CCAGCTACCC TGGACCAAGC TACCAGGGCT ACCACACCAT GCCGCCTCAG 
CCAGGGATGC CAGCAGCACC CTACCCAATG CAGTACCCAC CACCTTACCC AGCCCAGCCC 
ATGGGCCCAC CGGCCTACCA CGAGACCCTG GCTGGAGGAG CAGCCGCGCC CTACCCCGCC 
AGCCAGCCTC CTTACAACCC GGCCTACATG GATGCCCCGA AGGCGGCCCT CTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001259011.1
CDS320..733
Translation

Target ORF information:

RefSeq Version NM_001272082.1
Organism Homo sapiens(human)
Definition Homo sapiens shisa family member 5 (SHISA5), transcript variant 6, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 NM_001272082.1

ORF Insert Sequence:

1
61
121
181
241
301
361
ATGGGGTTCG GAGCGACCTT GGCCGTTGGC CTGACCATCT TTGTGCTGTC TGTCGTCACT 
ATCATCATCT GCTTCACCTG CTCCTGCTGC TGCCTTTACA AGACGTGCCG CCGACCACGT 
CCGGTTGTCA CCACCACCAC ATCCACCACT GTGGTGCATG CCCCTTATCC TCAGCCTCCA 
AGTGTGCCGC CCAGCTACCC TGGACCAAGC TACCAGGGCT ACCACACCAT GCCGCCTCAG 
CCAGGGATGC CAGCAGCACC CTACCCAATG CAGTACCCAC CACCTTACCC AGCCCAGCCC 
ATGGGCCCAC CGGCCTACCA CGAGACCCTG GCTGGAGGAG CAGCCGCGCC CTACCCCGCC 
AGCCAGCCTC CTTACAACCC GGCCTACATG GATGCCCCGA AGGCGGCCCT CTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu07269
Clone ID Related Accession (Same CDS sequence) NM_016479.4
Accession Version NM_016479.4 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 723bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2019-05-03
Organism Homo sapiens(human)
Product protein shisa-5 isoform a precursor
Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BI552637.1 and AF520698.1. On Jan 11, 2013 this sequence version replaced NM_016479.3. Transcript Variant: This variant (1) encodes the longest isoform (a). ##Evidence-Data-START## Transcript exon combination :: AF520698.1, SRR1803611.12805.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## RefSeq Select criteria :: based on conservation, longest protein ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end. 

1
61
121
181
241
301
361
421
481
541
601
661
721
ATGACTGCGC CGGTCCCCGC GCCGCGGATC CTGTTGCCGT TGCTGTTGCT GCTGCTGCTA 
ACGCCGCCTC CGGGTGCACG TGGTGAGGTG TGTATGGCTT CCCGTGGACT CAGCCTCTTC 
CCCGAGTCCT GTCCAGATTT CTGCTGTGGT ACCTGTGATG ACCAATACTG CTGCTCTGAC 
GTGCTGAAGA AATTTGTGTG GAGCGAGGAA AGGTGTGCTG TGCCTGAGGC CAGCGTGCCT 
GCCAGTGTAG AGCCGGTGGA GCAGCTGGGC TCGGCGCTGA GGTTTCGCCC TGGCTACAAC 
GACCCCATGT CAGGGTTCGG AGCGACCTTG GCCGTTGGCC TGACCATCTT TGTGCTGTCT 
GTCGTCACTA TCATCATCTG CTTCACCTGC TCCTGCTGCT GCCTTTACAA GACGTGCCGC 
CGACCACGTC CGGTTGTCAC CACCACCACA TCCACCACTG TGGTGCATGC CCCTTATCCT 
CAGCCTCCAA GTGTGCCGCC CAGCTACCCT GGACCAAGCT ACCAGGGCTA CCACACCATG 
CCGCCTCAGC CAGGGATGCC AGCAGCACCC TACCCAATGC AGTACCCACC ACCTTACCCA 
GCCCAGCCCA TGGGCCCACC GGCCTACCAC GAGACCCTGG CTGGAGGAGC AGCCGCGCCC 
TACCCCGCCA GCCAGCCTCC TTACAACCCG GCCTACATGG ATGCCCCGAA GGCGGCCCTC 
TGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_057563.3
CDS156..878
Translation

Target ORF information:

RefSeq Version NM_016479.4
Organism Homo sapiens(human)
Definition Homo sapiens shisa family member 5 (SHISA5), transcript variant 1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 NM_016479.4

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
ATGACTGCGC CGGTCCCCGC GCCGCGGATC CTGTTGCCGT TGCTGTTGCT GCTGCTGCTA 
ACGCCGCCTC CGGGTGCACG TGGTGAGGTG TGTATGGCTT CCCGTGGACT CAGCCTCTTC 
CCCGAGTCCT GTCCAGATTT CTGCTGTGGT ACCTGTGATG ACCAATACTG CTGCTCTGAC 
GTGCTGAAGA AATTTGTGTG GAGCGAGGAA AGGTGTGCTG TGCCTGAGGC CAGCGTGCCT 
GCCAGTGTAG AGCCGGTGGA GCAGCTGGGC TCGGCGCTGA GGTTTCGCCC TGGCTACAAC 
GACCCCATGT CAGGGTTCGG AGCGACCTTG GCCGTTGGCC TGACCATCTT TGTGCTGTCT 
GTCGTCACTA TCATCATCTG CTTCACCTGC TCCTGCTGCT GCCTTTACAA GACGTGCCGC 
CGACCACGTC CGGTTGTCAC CACCACCACA TCCACCACTG TGGTGCATGC CCCTTATCCT 
CAGCCTCCAA GTGTGCCGCC CAGCTACCCT GGACCAAGCT ACCAGGGCTA CCACACCATG 
CCGCCTCAGC CAGGGATGCC AGCAGCACCC TACCCAATGC AGTACCCACC ACCTTACCCA 
GCCCAGCCCA TGGGCCCACC GGCCTACCAC GAGACCCTGG CTGGAGGAGC AGCCGCGCCC 
TACCCCGCCA GCCAGCCTCC TTACAACCCG GCCTACATGG ATGCCCCGAA GGCGGCCCTC 
TGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu04069
Clone ID Related Accession (Same CDS sequence) NM_001272065.1
Accession Version NM_001272065.1 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 702bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2018-06-24
Organism Homo sapiens(human)
Product protein shisa-5 isoform b precursor
Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BI552637.1, DA009534.1, BX379507.2 and AF520698.1. Transcript Variant: This variant (2) uses an alternate in-frame splice site in the coding region, compared to variant 1. The encoded isoform (b) is shorter than isoform a. ##Evidence-Data-START## Transcript exon combination :: BX340061.2, BX379507.2 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end. 

1
61
121
181
241
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361
421
481
541
601
661
ATGACTGCGC CGGTCCCCGC GCCGCGGATC CTGTTGCCGT TGCTGTTGCT GCTGCTGCTA 
ACGCCGCCTC CGGGTGCACG TGGTGAGGTG TGTATGGCTT CCCGTGGACT CAGCCTCTTC 
CCCGAGTCCT GTCCAGATTT CTGCTGTGGT ACCTGTGATG ACCAATACTG CTGCTCTGAC 
GTGCTGAAGA AATTTGTGTG GAGCGAGGAA AGCGTGCCTG CCAGTGTAGA GCCGGTGGAG 
CAGCTGGGCT CGGCGCTGAG GTTTCGCCCT GGCTACAACG ACCCCATGTC AGGGTTCGGA 
GCGACCTTGG CCGTTGGCCT GACCATCTTT GTGCTGTCTG TCGTCACTAT CATCATCTGC 
TTCACCTGCT CCTGCTGCTG CCTTTACAAG ACGTGCCGCC GACCACGTCC GGTTGTCACC 
ACCACCACAT CCACCACTGT GGTGCATGCC CCTTATCCTC AGCCTCCAAG TGTGCCGCCC 
AGCTACCCTG GACCAAGCTA CCAGGGCTAC CACACCATGC CGCCTCAGCC AGGGATGCCA 
GCAGCACCCT ACCCAATGCA GTACCCACCA CCTTACCCAG CCCAGCCCAT GGGCCCACCG 
GCCTACCACG AGACCCTGGC TGGAGGAGCA GCCGCGCCCT ACCCCGCCAG CCAGCCTCCT 
TACAACCCGG CCTACATGGA TGCCCCGAAG GCGGCCCTCT GA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001258994.1
CDS156..857
Translation

Target ORF information:

RefSeq Version NM_001272065.1
Organism Homo sapiens(human)
Definition Homo sapiens shisa family member 5 (SHISA5), transcript variant 2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 NM_001272065.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
ATGACTGCGC CGGTCCCCGC GCCGCGGATC CTGTTGCCGT TGCTGTTGCT GCTGCTGCTA 
ACGCCGCCTC CGGGTGCACG TGGTGAGGTG TGTATGGCTT CCCGTGGACT CAGCCTCTTC 
CCCGAGTCCT GTCCAGATTT CTGCTGTGGT ACCTGTGATG ACCAATACTG CTGCTCTGAC 
GTGCTGAAGA AATTTGTGTG GAGCGAGGAA AGCGTGCCTG CCAGTGTAGA GCCGGTGGAG 
CAGCTGGGCT CGGCGCTGAG GTTTCGCCCT GGCTACAACG ACCCCATGTC AGGGTTCGGA 
GCGACCTTGG CCGTTGGCCT GACCATCTTT GTGCTGTCTG TCGTCACTAT CATCATCTGC 
TTCACCTGCT CCTGCTGCTG CCTTTACAAG ACGTGCCGCC GACCACGTCC GGTTGTCACC 
ACCACCACAT CCACCACTGT GGTGCATGCC CCTTATCCTC AGCCTCCAAG TGTGCCGCCC 
AGCTACCCTG GACCAAGCTA CCAGGGCTAC CACACCATGC CGCCTCAGCC AGGGATGCCA 
GCAGCACCCT ACCCAATGCA GTACCCACCA CCTTACCCAG CCCAGCCCAT GGGCCCACCG 
GCCTACCACG AGACCCTGGC TGGAGGAGCA GCCGCGCCCT ACCCCGCCAG CCAGCCTCCT 
TACAACCCGG CCTACATGGA TGCCCCGAAG GCGGCCCTCT GA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu04070
Clone ID Related Accession (Same CDS sequence) NM_001272067.1 , NM_001272066.1 , XM_011533796.1 , XM_006713188.3 , XM_005265205.1 , XM_006713188.2 , NM_001272068.1
Accession Version NM_001272067.1 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 630bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2018-06-24
Organism Homo sapiens(human)
Product protein shisa-5 isoform c
Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA020280.1, DA657994.1, AK056328.1, BX336842.2, BM929955.1 and AF520698.1. Transcript Variant: This variant (4) uses an alternate 5' exon and initiates translation at a downstream AUG, compared to variant 1. The encoded isoform (c) is shorter than isoform a. Variants 3, 4 and 5 encode the same protein. ##Evidence-Data-START## Transcript exon combination :: AK056328.1, SRR1803617.50633.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2155770 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end. 

1
61
121
181
241
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361
421
481
541
601
ATGGCTTCCC GTGGACTCAG CCTCTTCCCC GAGTCCTGTC CAGATTTCTG CTGTGGTACC 
TGTGATGACC AATACTGCTG CTCTGACGTG CTGAAGAAAT TTGTGTGGAG CGAGGAAAGG 
TGTGCTGTGC CTGAGGCCAG CGTGCCTGCC AGTGTAGAGC CGGTGGAGCA GCTGGGCTCG 
GCGCTGAGGT TTCGCCCTGG CTACAACGAC CCCATGTCAG GGTTCGGAGC GACCTTGGCC 
GTTGGCCTGA CCATCTTTGT GCTGTCTGTC GTCACTATCA TCATCTGCTT CACCTGCTCC 
TGCTGCTGCC TTTACAAGAC GTGCCGCCGA CCACGTCCGG TTGTCACCAC CACCACATCC 
ACCACTGTGG TGCATGCCCC TTATCCTCAG CCTCCAAGTG TGCCGCCCAG CTACCCTGGA 
CCAAGCTACC AGGGCTACCA CACCATGCCG CCTCAGCCAG GGATGCCAGC AGCACCCTAC 
CCAATGCAGT ACCCACCACC TTACCCAGCC CAGCCCATGG GCCCACCGGC CTACCACGAG 
ACCCTGGCTG GAGGAGCAGC CGCGCCCTAC CCCGCCAGCC AGCCTCCTTA CAACCCGGCC 
TACATGGATG CCCCGAAGGC GGCCCTCTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001258996.1
CDS410..1039
Translation

Target ORF information:

RefSeq Version NM_001272067.1
Organism Homo sapiens(human)
Definition Homo sapiens shisa family member 5 (SHISA5), transcript variant 4, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 NM_001272067.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
ATGGCTTCCC GTGGACTCAG CCTCTTCCCC GAGTCCTGTC CAGATTTCTG CTGTGGTACC 
TGTGATGACC AATACTGCTG CTCTGACGTG CTGAAGAAAT TTGTGTGGAG CGAGGAAAGG 
TGTGCTGTGC CTGAGGCCAG CGTGCCTGCC AGTGTAGAGC CGGTGGAGCA GCTGGGCTCG 
GCGCTGAGGT TTCGCCCTGG CTACAACGAC CCCATGTCAG GGTTCGGAGC GACCTTGGCC 
GTTGGCCTGA CCATCTTTGT GCTGTCTGTC GTCACTATCA TCATCTGCTT CACCTGCTCC 
TGCTGCTGCC TTTACAAGAC GTGCCGCCGA CCACGTCCGG TTGTCACCAC CACCACATCC 
ACCACTGTGG TGCATGCCCC TTATCCTCAG CCTCCAAGTG TGCCGCCCAG CTACCCTGGA 
CCAAGCTACC AGGGCTACCA CACCATGCCG CCTCAGCCAG GGATGCCAGC AGCACCCTAC 
CCAATGCAGT ACCCACCACC TTACCCAGCC CAGCCCATGG GCCCACCGGC CTACCACGAG 
ACCCTGGCTG GAGGAGCAGC CGCGCCCTAC CCCGCCAGCC AGCCTCCTTA CAACCCGGCC 
TACATGGATG CCCCGAAGGC GGCCCTCTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu04070
Clone ID Related Accession (Same CDS sequence) NM_001272067.1 , NM_001272066.1 , XM_011533796.1 , XM_006713188.3 , XM_005265205.1 , XM_006713188.2 , NM_001272068.1
Accession Version NM_001272066.1 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 630bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2018-06-24
Organism Homo sapiens(human)
Product protein shisa-5 isoform c
Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA045437.1, DA025113.1, BX344125.2, AK056328.1 and AF520698.1. This sequence is a reference standard in the RefSeqGene project. Transcript Variant: This variant (3) uses an alternate 5' exon and initiates translation at a downstream AUG, compared to variant 1. The encoded isoform (c) is shorter than isoform a. Variants 3, 4 and 5 encode the same protein. ##Evidence-Data-START## Transcript exon combination :: SRR1803616.2689.1, BX380934.2 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end. 

1
61
121
181
241
301
361
421
481
541
601
ATGGCTTCCC GTGGACTCAG CCTCTTCCCC GAGTCCTGTC CAGATTTCTG CTGTGGTACC 
TGTGATGACC AATACTGCTG CTCTGACGTG CTGAAGAAAT TTGTGTGGAG CGAGGAAAGG 
TGTGCTGTGC CTGAGGCCAG CGTGCCTGCC AGTGTAGAGC CGGTGGAGCA GCTGGGCTCG 
GCGCTGAGGT TTCGCCCTGG CTACAACGAC CCCATGTCAG GGTTCGGAGC GACCTTGGCC 
GTTGGCCTGA CCATCTTTGT GCTGTCTGTC GTCACTATCA TCATCTGCTT CACCTGCTCC 
TGCTGCTGCC TTTACAAGAC GTGCCGCCGA CCACGTCCGG TTGTCACCAC CACCACATCC 
ACCACTGTGG TGCATGCCCC TTATCCTCAG CCTCCAAGTG TGCCGCCCAG CTACCCTGGA 
CCAAGCTACC AGGGCTACCA CACCATGCCG CCTCAGCCAG GGATGCCAGC AGCACCCTAC 
CCAATGCAGT ACCCACCACC TTACCCAGCC CAGCCCATGG GCCCACCGGC CTACCACGAG 
ACCCTGGCTG GAGGAGCAGC CGCGCCCTAC CCCGCCAGCC AGCCTCCTTA CAACCCGGCC 
TACATGGATG CCCCGAAGGC GGCCCTCTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001258995.1
CDS215..844
Translation

Target ORF information:

RefSeq Version NM_001272066.1
Organism Homo sapiens(human)
Definition Homo sapiens shisa family member 5 (SHISA5), transcript variant 3, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 NM_001272066.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
ATGGCTTCCC GTGGACTCAG CCTCTTCCCC GAGTCCTGTC CAGATTTCTG CTGTGGTACC 
TGTGATGACC AATACTGCTG CTCTGACGTG CTGAAGAAAT TTGTGTGGAG CGAGGAAAGG 
TGTGCTGTGC CTGAGGCCAG CGTGCCTGCC AGTGTAGAGC CGGTGGAGCA GCTGGGCTCG 
GCGCTGAGGT TTCGCCCTGG CTACAACGAC CCCATGTCAG GGTTCGGAGC GACCTTGGCC 
GTTGGCCTGA CCATCTTTGT GCTGTCTGTC GTCACTATCA TCATCTGCTT CACCTGCTCC 
TGCTGCTGCC TTTACAAGAC GTGCCGCCGA CCACGTCCGG TTGTCACCAC CACCACATCC 
ACCACTGTGG TGCATGCCCC TTATCCTCAG CCTCCAAGTG TGCCGCCCAG CTACCCTGGA 
CCAAGCTACC AGGGCTACCA CACCATGCCG CCTCAGCCAG GGATGCCAGC AGCACCCTAC 
CCAATGCAGT ACCCACCACC TTACCCAGCC CAGCCCATGG GCCCACCGGC CTACCACGAG 
ACCCTGGCTG GAGGAGCAGC CGCGCCCTAC CCCGCCAGCC AGCCTCCTTA CAACCCGGCC 
TACATGGATG CCCCGAAGGC GGCCCTCTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu04070
Clone ID Related Accession (Same CDS sequence) NM_001272067.1 , NM_001272066.1 , XM_011533796.1 , XM_006713188.3 , XM_005265205.1 , XM_006713188.2 , NM_001272068.1
Accession Version NM_001272068.1 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 630bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2018-06-24
Organism Homo sapiens(human)
Product protein shisa-5 isoform c
Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AK124652.1, BM929955.1 and AF520698.1. Transcript Variant: This variant (5) uses two alternate exons in the 5' UTR and initiates translation at a downstream AUG, compared to variant 1. The encoded isoform (c) is shorter than isoform a. Variants 3, 4 and 5 encode the same protein. ##Evidence-Data-START## Transcript exon combination :: AK124652.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end. 

1
61
121
181
241
301
361
421
481
541
601
ATGGCTTCCC GTGGACTCAG CCTCTTCCCC GAGTCCTGTC CAGATTTCTG CTGTGGTACC 
TGTGATGACC AATACTGCTG CTCTGACGTG CTGAAGAAAT TTGTGTGGAG CGAGGAAAGG 
TGTGCTGTGC CTGAGGCCAG CGTGCCTGCC AGTGTAGAGC CGGTGGAGCA GCTGGGCTCG 
GCGCTGAGGT TTCGCCCTGG CTACAACGAC CCCATGTCAG GGTTCGGAGC GACCTTGGCC 
GTTGGCCTGA CCATCTTTGT GCTGTCTGTC GTCACTATCA TCATCTGCTT CACCTGCTCC 
TGCTGCTGCC TTTACAAGAC GTGCCGCCGA CCACGTCCGG TTGTCACCAC CACCACATCC 
ACCACTGTGG TGCATGCCCC TTATCCTCAG CCTCCAAGTG TGCCGCCCAG CTACCCTGGA 
CCAAGCTACC AGGGCTACCA CACCATGCCG CCTCAGCCAG GGATGCCAGC AGCACCCTAC 
CCAATGCAGT ACCCACCACC TTACCCAGCC CAGCCCATGG GCCCACCGGC CTACCACGAG 
ACCCTGGCTG GAGGAGCAGC CGCGCCCTAC CCCGCCAGCC AGCCTCCTTA CAACCCGGCC 
TACATGGATG CCCCGAAGGC GGCCCTCTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001258997.1
CDS447..1076
Translation

Target ORF information:

RefSeq Version NM_001272068.1
Organism Homo sapiens(human)
Definition Homo sapiens shisa family member 5 (SHISA5), transcript variant 5, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 NM_001272068.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
ATGGCTTCCC GTGGACTCAG CCTCTTCCCC GAGTCCTGTC CAGATTTCTG CTGTGGTACC 
TGTGATGACC AATACTGCTG CTCTGACGTG CTGAAGAAAT TTGTGTGGAG CGAGGAAAGG 
TGTGCTGTGC CTGAGGCCAG CGTGCCTGCC AGTGTAGAGC CGGTGGAGCA GCTGGGCTCG 
GCGCTGAGGT TTCGCCCTGG CTACAACGAC CCCATGTCAG GGTTCGGAGC GACCTTGGCC 
GTTGGCCTGA CCATCTTTGT GCTGTCTGTC GTCACTATCA TCATCTGCTT CACCTGCTCC 
TGCTGCTGCC TTTACAAGAC GTGCCGCCGA CCACGTCCGG TTGTCACCAC CACCACATCC 
ACCACTGTGG TGCATGCCCC TTATCCTCAG CCTCCAAGTG TGCCGCCCAG CTACCCTGGA 
CCAAGCTACC AGGGCTACCA CACCATGCCG CCTCAGCCAG GGATGCCAGC AGCACCCTAC 
CCAATGCAGT ACCCACCACC TTACCCAGCC CAGCCCATGG GCCCACCGGC CTACCACGAG 
ACCCTGGCTG GAGGAGCAGC CGCGCCCTAC CCCGCCAGCC AGCCTCCTTA CAACCCGGCC 
TACATGGATG CCCCGAAGGC GGCCCTCTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu04073
Clone ID Related Accession (Same CDS sequence) NM_001272083.1 , NM_001272083.2
Accession Version NM_001272083.1 Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 357bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2018-06-24
Organism Homo sapiens(human)
Product protein shisa-5 isoform e
Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA046900.1, AL117413.1 and AF520698.1. Transcript Variant: This variant (7) contains an alternate 5' terminal exon, initiates translation at an alternate start codon, and differs at the 3' end compared to variant 1. The encoded isoform (e) has distinct N- and C- termini and is shorter than isoform a. ##Evidence-Data-START## Transcript exon combination :: SRR1803615.62318.1, AL117413.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2147596, SAMEA2149178 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end. 

1
61
121
181
241
301
ATGGGGTTCG GAGCGACCTT GGCCGTTGGC CTGACCATCT TTGTGCTGTC TGTCGTCACT 
ATCATCATCT GCTTCACCTG CTCCTGCTGC TGCCTTTACA AGACGTGCCG CCGACCACGT 
CCGGTTGTCA CCACCACCAC ATCCACCACT GTGGTGCATG CCCCTTATCC TCAGCCTCCA 
AGTGTGCCGC CCAGCTACCC TGGACCAAGC TACCAGGGCT ACCACACCAT GCCGCCTCAG 
CCAGGGATGC CAGCAGCACC CTACCCAATG CAGTACCCAC CACCTTACCC AGCCCAGCCC 
ATGGGCCCAC CGGCCTACCA CGAGACCCTG GCTGGTGAGT GCCCCTGCCA ACTCTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001259012.1
CDS320..676
Translation

Target ORF information:

RefSeq Version NM_001272083.1
Organism Homo sapiens(human)
Definition Homo sapiens shisa family member 5 (SHISA5), transcript variant 7, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 NM_001272083.1

ORF Insert Sequence:

1
61
121
181
241
301
ATGGGGTTCG GAGCGACCTT GGCCGTTGGC CTGACCATCT TTGTGCTGTC TGTCGTCACT 
ATCATCATCT GCTTCACCTG CTCCTGCTGC TGCCTTTACA AGACGTGCCG CCGACCACGT 
CCGGTTGTCA CCACCACCAC ATCCACCACT GTGGTGCATG CCCCTTATCC TCAGCCTCCA 
AGTGTGCCGC CCAGCTACCC TGGACCAAGC TACCAGGGCT ACCACACCAT GCCGCCTCAG 
CCAGGGATGC CAGCAGCACC CTACCCAATG CAGTACCCAC CACCTTACCC AGCCCAGCCC 
ATGGGCCCAC CGGCCTACCA CGAGACCCTG GCTGGTGAGT GCCCCTGCCA ACTCTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu04073
Clone ID Related Accession (Same CDS sequence) NM_001272083.1 , NM_001272083.2
Accession Version NM_001272083.2 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 357bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2019-06-01
Organism Homo sapiens(human)
Product protein shisa-5 isoform e
Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA046900.1, AL117413.1 and AF520698.1. On Jun 2, 2019 this sequence version replaced NM_001272083.1. Transcript Variant: This variant (7) contains an alternate 5' terminal exon, initiates translation at an alternate start codon, and differs at the 3' end compared to variant 1. The encoded isoform (e) has distinct N- and C- termini and is shorter than isoform a. ##Evidence-Data-START## Transcript exon combination :: SRR1803615.62318.1, SRR1660805.246925.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2147596, SAMEA2149178 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end. 

1
61
121
181
241
301
ATGGGGTTCG GAGCGACCTT GGCCGTTGGC CTGACCATCT TTGTGCTGTC TGTCGTCACT 
ATCATCATCT GCTTCACCTG CTCCTGCTGC TGCCTTTACA AGACGTGCCG CCGACCACGT 
CCGGTTGTCA CCACCACCAC ATCCACCACT GTGGTGCATG CCCCTTATCC TCAGCCTCCA 
AGTGTGCCGC CCAGCTACCC TGGACCAAGC TACCAGGGCT ACCACACCAT GCCGCCTCAG 
CCAGGGATGC CAGCAGCACC CTACCCAATG CAGTACCCAC CACCTTACCC AGCCCAGCCC 
ATGGGCCCAC CGGCCTACCA CGAGACCCTG GCTGGTGAGT GCCCCTGCCA ACTCTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001259012.1
CDS145..501
Translation

Target ORF information:

RefSeq Version NM_001272083.2
Organism Homo sapiens(human)
Definition Homo sapiens shisa family member 5 (SHISA5), transcript variant 7, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 NM_001272083.2

ORF Insert Sequence:

1
61
121
181
241
301
ATGGGGTTCG GAGCGACCTT GGCCGTTGGC CTGACCATCT TTGTGCTGTC TGTCGTCACT 
ATCATCATCT GCTTCACCTG CTCCTGCTGC TGCCTTTACA AGACGTGCCG CCGACCACGT 
CCGGTTGTCA CCACCACCAC ATCCACCACT GTGGTGCATG CCCCTTATCC TCAGCCTCCA 
AGTGTGCCGC CCAGCTACCC TGGACCAAGC TACCAGGGCT ACCACACCAT GCCGCCTCAG 
CCAGGGATGC CAGCAGCACC CTACCCAATG CAGTACCCAC CACCTTACCC AGCCCAGCCC 
ATGGGCCCAC CGGCCTACCA CGAGACCCTG GCTGGTGAGT GCCCCTGCCA ACTCTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu04070
Clone ID Related Accession (Same CDS sequence) NM_001272067.1 , NM_001272066.1 , XM_011533796.1 , XM_006713188.3 , XM_005265205.1 , XM_006713188.2 , NM_001272068.1
Accession Version XM_011533796.1 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 630bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2019-12-05
Organism Homo sapiens(human)
Product protein shisa-5 isoform X1
Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000003.12) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

1
61
121
181
241
301
361
421
481
541
601
ATGGCTTCCC GTGGACTCAG CCTCTTCCCC GAGTCCTGTC CAGATTTCTG CTGTGGTACC 
TGTGATGACC AATACTGCTG CTCTGACGTG CTGAAGAAAT TTGTGTGGAG CGAGGAAAGG 
TGTGCTGTGC CTGAGGCCAG CGTGCCTGCC AGTGTAGAGC CGGTGGAGCA GCTGGGCTCG 
GCGCTGAGGT TTCGCCCTGG CTACAACGAC CCCATGTCAG GGTTCGGAGC GACCTTGGCC 
GTTGGCCTGA CCATCTTTGT GCTGTCTGTC GTCACTATCA TCATCTGCTT CACCTGCTCC 
TGCTGCTGCC TTTACAAGAC GTGCCGCCGA CCACGTCCGG TTGTCACCAC CACCACATCC 
ACCACTGTGG TGCATGCCCC TTATCCTCAG CCTCCAAGTG TGCCGCCCAG CTACCCTGGA 
CCAAGCTACC AGGGCTACCA CACCATGCCG CCTCAGCCAG GGATGCCAGC AGCACCCTAC 
CCAATGCAGT ACCCACCACC TTACCCAGCC CAGCCCATGG GCCCACCGGC CTACCACGAG 
ACCCTGGCTG GAGGAGCAGC CGCGCCCTAC CCCGCCAGCC AGCCTCCTTA CAACCCGGCC 
TACATGGATG CCCCGAAGGC GGCCCTCTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_011532098.1
CDS239..868
Translation

Target ORF information:

RefSeq Version XM_011533796.1
Organism Homo sapiens(human)
Definition Homo sapiens shisa family member 5 (SHISA5), transcript variant X1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 XM_011533796.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
ATGGCTTCCC GTGGACTCAG CCTCTTCCCC GAGTCCTGTC CAGATTTCTG CTGTGGTACC 
TGTGATGACC AATACTGCTG CTCTGACGTG CTGAAGAAAT TTGTGTGGAG CGAGGAAAGG 
TGTGCTGTGC CTGAGGCCAG CGTGCCTGCC AGTGTAGAGC CGGTGGAGCA GCTGGGCTCG 
GCGCTGAGGT TTCGCCCTGG CTACAACGAC CCCATGTCAG GGTTCGGAGC GACCTTGGCC 
GTTGGCCTGA CCATCTTTGT GCTGTCTGTC GTCACTATCA TCATCTGCTT CACCTGCTCC 
TGCTGCTGCC TTTACAAGAC GTGCCGCCGA CCACGTCCGG TTGTCACCAC CACCACATCC 
ACCACTGTGG TGCATGCCCC TTATCCTCAG CCTCCAAGTG TGCCGCCCAG CTACCCTGGA 
CCAAGCTACC AGGGCTACCA CACCATGCCG CCTCAGCCAG GGATGCCAGC AGCACCCTAC 
CCAATGCAGT ACCCACCACC TTACCCAGCC CAGCCCATGG GCCCACCGGC CTACCACGAG 
ACCCTGGCTG GAGGAGCAGC CGCGCCCTAC CCCGCCAGCC AGCCTCCTTA CAACCCGGCC 
TACATGGATG CCCCGAAGGC GGCCCTCTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu04070
Clone ID Related Accession (Same CDS sequence) NM_001272067.1 , NM_001272066.1 , XM_011533796.1 , XM_006713188.3 , XM_005265205.1 , XM_006713188.2 , NM_001272068.1
Accession Version XM_006713188.3 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 630bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2019-12-05
Organism Homo sapiens(human)
Product protein shisa-5 isoform X1
Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000003.12) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_006713188.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

1
61
121
181
241
301
361
421
481
541
601
ATGGCTTCCC GTGGACTCAG CCTCTTCCCC GAGTCCTGTC CAGATTTCTG CTGTGGTACC 
TGTGATGACC AATACTGCTG CTCTGACGTG CTGAAGAAAT TTGTGTGGAG CGAGGAAAGG 
TGTGCTGTGC CTGAGGCCAG CGTGCCTGCC AGTGTAGAGC CGGTGGAGCA GCTGGGCTCG 
GCGCTGAGGT TTCGCCCTGG CTACAACGAC CCCATGTCAG GGTTCGGAGC GACCTTGGCC 
GTTGGCCTGA CCATCTTTGT GCTGTCTGTC GTCACTATCA TCATCTGCTT CACCTGCTCC 
TGCTGCTGCC TTTACAAGAC GTGCCGCCGA CCACGTCCGG TTGTCACCAC CACCACATCC 
ACCACTGTGG TGCATGCCCC TTATCCTCAG CCTCCAAGTG TGCCGCCCAG CTACCCTGGA 
CCAAGCTACC AGGGCTACCA CACCATGCCG CCTCAGCCAG GGATGCCAGC AGCACCCTAC 
CCAATGCAGT ACCCACCACC TTACCCAGCC CAGCCCATGG GCCCACCGGC CTACCACGAG 
ACCCTGGCTG GAGGAGCAGC CGCGCCCTAC CCCGCCAGCC AGCCTCCTTA CAACCCGGCC 
TACATGGATG CCCCGAAGGC GGCCCTCTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_006713251.1
CDS145..774
Translation

Target ORF information:

RefSeq Version XM_006713188.3
Organism Homo sapiens(human)
Definition Homo sapiens shisa family member 5 (SHISA5), transcript variant X3, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 XM_006713188.3

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
ATGGCTTCCC GTGGACTCAG CCTCTTCCCC GAGTCCTGTC CAGATTTCTG CTGTGGTACC 
TGTGATGACC AATACTGCTG CTCTGACGTG CTGAAGAAAT TTGTGTGGAG CGAGGAAAGG 
TGTGCTGTGC CTGAGGCCAG CGTGCCTGCC AGTGTAGAGC CGGTGGAGCA GCTGGGCTCG 
GCGCTGAGGT TTCGCCCTGG CTACAACGAC CCCATGTCAG GGTTCGGAGC GACCTTGGCC 
GTTGGCCTGA CCATCTTTGT GCTGTCTGTC GTCACTATCA TCATCTGCTT CACCTGCTCC 
TGCTGCTGCC TTTACAAGAC GTGCCGCCGA CCACGTCCGG TTGTCACCAC CACCACATCC 
ACCACTGTGG TGCATGCCCC TTATCCTCAG CCTCCAAGTG TGCCGCCCAG CTACCCTGGA 
CCAAGCTACC AGGGCTACCA CACCATGCCG CCTCAGCCAG GGATGCCAGC AGCACCCTAC 
CCAATGCAGT ACCCACCACC TTACCCAGCC CAGCCCATGG GCCCACCGGC CTACCACGAG 
ACCCTGGCTG GAGGAGCAGC CGCGCCCTAC CCCGCCAGCC AGCCTCCTTA CAACCCGGCC 
TACATGGATG CCCCGAAGGC GGCCCTCTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu04070
Clone ID Related Accession (Same CDS sequence) NM_001272067.1 , NM_001272066.1 , XM_011533796.1 , XM_006713188.3 , XM_005265205.1 , XM_006713188.2 , NM_001272068.1
Accession Version XM_005265205.1 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 630bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2019-12-05
Organism Homo sapiens(human)
Product protein shisa-5 isoform X1
Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000003.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

1
61
121
181
241
301
361
421
481
541
601
ATGGCTTCCC GTGGACTCAG CCTCTTCCCC GAGTCCTGTC CAGATTTCTG CTGTGGTACC 
TGTGATGACC AATACTGCTG CTCTGACGTG CTGAAGAAAT TTGTGTGGAG CGAGGAAAGG 
TGTGCTGTGC CTGAGGCCAG CGTGCCTGCC AGTGTAGAGC CGGTGGAGCA GCTGGGCTCG 
GCGCTGAGGT TTCGCCCTGG CTACAACGAC CCCATGTCAG GGTTCGGAGC GACCTTGGCC 
GTTGGCCTGA CCATCTTTGT GCTGTCTGTC GTCACTATCA TCATCTGCTT CACCTGCTCC 
TGCTGCTGCC TTTACAAGAC GTGCCGCCGA CCACGTCCGG TTGTCACCAC CACCACATCC 
ACCACTGTGG TGCATGCCCC TTATCCTCAG CCTCCAAGTG TGCCGCCCAG CTACCCTGGA 
CCAAGCTACC AGGGCTACCA CACCATGCCG CCTCAGCCAG GGATGCCAGC AGCACCCTAC 
CCAATGCAGT ACCCACCACC TTACCCAGCC CAGCCCATGG GCCCACCGGC CTACCACGAG 
ACCCTGGCTG GAGGAGCAGC CGCGCCCTAC CCCGCCAGCC AGCCTCCTTA CAACCCGGCC 
TACATGGATG CCCCGAAGGC GGCCCTCTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_005265262.1
CDS497..1126
Translation

Target ORF information:

RefSeq Version XM_005265205.1
Organism Homo sapiens(human)
Definition Homo sapiens shisa family member 5 (SHISA5), transcript variant X2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 XM_005265205.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
ATGGCTTCCC GTGGACTCAG CCTCTTCCCC GAGTCCTGTC CAGATTTCTG CTGTGGTACC 
TGTGATGACC AATACTGCTG CTCTGACGTG CTGAAGAAAT TTGTGTGGAG CGAGGAAAGG 
TGTGCTGTGC CTGAGGCCAG CGTGCCTGCC AGTGTAGAGC CGGTGGAGCA GCTGGGCTCG 
GCGCTGAGGT TTCGCCCTGG CTACAACGAC CCCATGTCAG GGTTCGGAGC GACCTTGGCC 
GTTGGCCTGA CCATCTTTGT GCTGTCTGTC GTCACTATCA TCATCTGCTT CACCTGCTCC 
TGCTGCTGCC TTTACAAGAC GTGCCGCCGA CCACGTCCGG TTGTCACCAC CACCACATCC 
ACCACTGTGG TGCATGCCCC TTATCCTCAG CCTCCAAGTG TGCCGCCCAG CTACCCTGGA 
CCAAGCTACC AGGGCTACCA CACCATGCCG CCTCAGCCAG GGATGCCAGC AGCACCCTAC 
CCAATGCAGT ACCCACCACC TTACCCAGCC CAGCCCATGG GCCCACCGGC CTACCACGAG 
ACCCTGGCTG GAGGAGCAGC CGCGCCCTAC CCCGCCAGCC AGCCTCCTTA CAACCCGGCC 
TACATGGATG CCCCGAAGGC GGCCCTCTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu04070
Clone ID Related Accession (Same CDS sequence) NM_001272067.1 , NM_001272066.1 , XM_011533796.1 , XM_006713188.3 , XM_005265205.1 , XM_006713188.2 , NM_001272068.1
Accession Version XM_006713188.2 Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 630bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2016-06-05
Organism Homo sapiens(human)
Product protein shisa-5 isoform X1
Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000003.12) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 12, 2015 this sequence version replaced XM_006713188.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

1
61
121
181
241
301
361
421
481
541
601
ATGGCTTCCC GTGGACTCAG CCTCTTCCCC GAGTCCTGTC CAGATTTCTG CTGTGGTACC 
TGTGATGACC AATACTGCTG CTCTGACGTG CTGAAGAAAT TTGTGTGGAG CGAGGAAAGG 
TGTGCTGTGC CTGAGGCCAG CGTGCCTGCC AGTGTAGAGC CGGTGGAGCA GCTGGGCTCG 
GCGCTGAGGT TTCGCCCTGG CTACAACGAC CCCATGTCAG GGTTCGGAGC GACCTTGGCC 
GTTGGCCTGA CCATCTTTGT GCTGTCTGTC GTCACTATCA TCATCTGCTT CACCTGCTCC 
TGCTGCTGCC TTTACAAGAC GTGCCGCCGA CCACGTCCGG TTGTCACCAC CACCACATCC 
ACCACTGTGG TGCATGCCCC TTATCCTCAG CCTCCAAGTG TGCCGCCCAG CTACCCTGGA 
CCAAGCTACC AGGGCTACCA CACCATGCCG CCTCAGCCAG GGATGCCAGC AGCACCCTAC 
CCAATGCAGT ACCCACCACC TTACCCAGCC CAGCCCATGG GCCCACCGGC CTACCACGAG 
ACCCTGGCTG GAGGAGCAGC CGCGCCCTAC CCCGCCAGCC AGCCTCCTTA CAACCCGGCC 
TACATGGATG CCCCGAAGGC GGCCCTCTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_006713251.1
CDS150..779
Translation

Target ORF information:

RefSeq Version XM_006713188.2
Organism Homo sapiens(human)
Definition Homo sapiens shisa family member 5 (SHISA5), transcript variant X3, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 XM_006713188.2

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
ATGGCTTCCC GTGGACTCAG CCTCTTCCCC GAGTCCTGTC CAGATTTCTG CTGTGGTACC 
TGTGATGACC AATACTGCTG CTCTGACGTG CTGAAGAAAT TTGTGTGGAG CGAGGAAAGG 
TGTGCTGTGC CTGAGGCCAG CGTGCCTGCC AGTGTAGAGC CGGTGGAGCA GCTGGGCTCG 
GCGCTGAGGT TTCGCCCTGG CTACAACGAC CCCATGTCAG GGTTCGGAGC GACCTTGGCC 
GTTGGCCTGA CCATCTTTGT GCTGTCTGTC GTCACTATCA TCATCTGCTT CACCTGCTCC 
TGCTGCTGCC TTTACAAGAC GTGCCGCCGA CCACGTCCGG TTGTCACCAC CACCACATCC 
ACCACTGTGG TGCATGCCCC TTATCCTCAG CCTCCAAGTG TGCCGCCCAG CTACCCTGGA 
CCAAGCTACC AGGGCTACCA CACCATGCCG CCTCAGCCAG GGATGCCAGC AGCACCCTAC 
CCAATGCAGT ACCCACCACC TTACCCAGCC CAGCCCATGG GCCCACCGGC CTACCACGAG 
ACCCTGGCTG GAGGAGCAGC CGCGCCCTAC CCCGCCAGCC AGCCTCCTTA CAACCCGGCC 
TACATGGATG CCCCGAAGGC GGCCCTCTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

PubMed

book

Interferon-inducible protein SCOTIN interferes with HCV replication through the autolysosomal degradation of NS5A.
Nature communications710631(2016 Feb)
Kim N,Kim MJ,Sung PS,Bae YC,Shin EC,Yoo JY


book

Identification of neuroglycan C and interacting partners as potential susceptibility genes for schizophrenia in a Southern Chinese population.
American journal of medical genetics. Part B, Neuropsychiatric genetics : the official publication of the International Society of Psychiatric Genetics153B(1)103-13(2010 Jan)
So HC,Fong PY,Chen RY,Hui TC,Ng MY,Cherny SS,Mak WW,Cheung EF,Chan RC,Chen EY,Li T,Sham PC


book

The calcium binding protein ALG-2 binds and stabilizes Scotin, a p53-inducible gene product localized at the endoplasmic reticulum membrane.
Archives of biochemistry and biophysics467(1)87-94(2007 Nov)
Draeby I,Woods YL,la Cour JM,Mollerup J,Bourdon JC,Berchtold MW


book

WWOX binds the specific proline-rich ligand PPXY: identification of candidate interacting proteins.
Oncogene23(29)5049-55(2004 Jun)
Ludes-Meyers JH,Kil H,Bednarek AK,Drake J,Bedford MT,Aldaz CM


book

Large-scale identification and characterization of human genes that activate NF-kappaB and MAPK signaling pathways.
Oncogene22(21)3307-18(2003 May)
Matsuda A,Suzuki Y,Honda G,Muramatsu S,Matsuzaki O,Nagano Y,Doi T,Shimotohno K,Harada T,Nishida E,Hayashi H,Sugano S