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PKD2 cDNA ORF clone, Homo sapiens (human)

Gene Symbol PKD2
Entrez Gene ID 5311
Full Name polycystic kidney disease 2 (autosomal dominant)
Synonyms APKD2, PC2, PKD4, Pc-2, TRPP2
Gene Type protein-coding
Organism Homo sapiens (human)
Genome

4

4q22.1

Summary This gene encodes a member of the polycystin protein family. The encoded protein is a multi-pass membrane protein that functions as a calcium permeable cation channel, and is involved in calcium transport and calcium signaling in renal epithelial cells. This protein interacts with polycystin 1, and they may be partners in a common signaling cascade involved in tubular morphogenesis. Mutations in this gene are associated with autosomal dominant polycystic kidney disease type 2. [provided by RefSeq, Mar 2011]. lac of sum
Disorder MIM:

173910

Disorder Html: Polycystic kidney disease 2, 613095 (3)

mRNA and Protein(s)

mRNA Protein Name
NM_000297 NP_000288 polycystin-2
XM_011532028 XP_011530330 polycystin-2 isoform X1
XM_011532029 XP_011530331 polycystin-2 isoform X2
XM_011532030 XP_011530332 polycystin-2 isoform X3



Homo sapiens (human) PKD2 NP_000288.1
Pan troglodytes (chimpanzee) PKD2 XP_003310458.1
Macaca mulatta (Rhesus monkey) PKD2 XP_001099242.1
Canis lupus familiaris (dog) PKD2 XP_544974.2
Bos taurus (cattle) PKD2 NP_001039777.1
Mus musculus (house mouse) Pkd2 NP_032887.3
Rattus norvegicus (Norway rat) Pkd2 NP_001178863.1
Gallus gallus (chicken) PKD2 NP_001026311.1
Danio rerio (zebrafish) pkd2 NP_001002310.1
Caenorhabditis elegans pkd-2 NP_502838.3
Xenopus (Silurana) tropicalis (western clawed frog) pkd2 XP_002938673.2


GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following PKD2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the PKD2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID RefSeq Accession Definition **Vector *Turnaround time Price Select
OHu26897
NM_000297 Homo sapiens polycystic kidney disease 2 (autosomal dominant) (PKD2), mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
$189.50-$265.30
$379.00
OHu59727 XM_011532028 PREDICTED: Homo sapiens polycystic kidney disease 2 (autosomal dominant) (PKD2), transcript variant X1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector TBD $475.30
$679.00
OHu59728 XM_011532029 PREDICTED: Homo sapiens polycystic kidney disease 2 (autosomal dominant) (PKD2), transcript variant X2, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 5-7 $391.30
$559.00
OHu59729 XM_011532030 PREDICTED: Homo sapiens polycystic kidney disease 2 (autosomal dominant) (PKD2), transcript variant X3, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 12-14 $391.30
$559.00
Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone 30% OFF
2-4 Clone 40% OFF
5 or more Clone 50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.


CloneID OHu26897
Clone ID Related Accession (Same CDS sequence) NM_000297
Accession Version NM_000297.3 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 2907bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 02-AUG-2015
Organism Homo sapiens (human)
Product polycystin-2
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BC112263.1, AF054992.1, AF113693.1 and AW600307.1. This sequence is a reference standard in the RefSeqGene project. On Mar 3, 2011 this sequence version replaced gi:33286447. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC112261.1, U50928.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
ATGGTGAACT CCAGTCGCGT GCAGCCTCAG CAGCCCGGGG ACGCCAAGCG GCCGCCCGCG 
CCCCGCGCGC CGGACCCGGG CCGGCTGATG GCTGGCTGCG CGGCCGTGGG CGCCAGCCTC
GCCGCCCCGG GCGGCCTCTG CGAGCAGCGG GGCCTGGAGA TCGAGATGCA GCGCATCCGG
CAGGCGGCCG CGCGGGACCC CCCGGCCGGA GCCGCGGCCT CCCCTTCTCC TCCGCTCTCG
TCGTGCTCCC GGCAGGCGTG GAGCCGCGAT AACCCCGGCT TCGAGGCCGA GGAGGAGGAG
GAGGAGGTGG AAGGGGAAGA AGGCGGAATG GTGGTGGAGA TGGACGTAGA GTGGCGCCCG
GGCAGCCGGA GGTCGGCCGC CTCCTCGGCC GTGAGCTCCG TGGGCGCGCG GAGCCGGGGG
CTTGGGGGCT ACCACGGCGC GGGCCACCCG AGCGGGAGGC GGCGCCGGCG AGAGGACCAG
GGCCCGCCGT GCCCCAGCCC AGTCGGCGGC GGGGACCCGC TGCATCGCCA CCTCCCCCTG
GAAGGGCAGC CGCCCCGAGT GGCCTGGGCG GAGAGGCTGG TTCGCGGGCT GCGAGGTCTC
TGGGGAACAA GACTCATGGA GGAAAGCAGC ACTAACCGAG AGAAATACCT TAAAAGTGTT
TTACGGGAAC TGGTCACATA CCTCCTTTTT CTCATAGTCT TGTGCATCTT GACCTACGGC
ATGATGAGCT CCAATGTGTA CTACTACACC CGGATGATGT CACAGCTCTT CCTAGACACC
CCCGTGTCCA AAACGGAGAA AACTAACTTT AAAACTCTGT CTTCCATGGA AGACTTCTGG
AAGTTCACAG AAGGCTCCTT ATTGGATGGG CTGTACTGGA AGATGCAGCC CAGCAACCAG
ACTGAAGCTG ACAACCGAAG TTTCATCTTC TATGAGAACC TGCTGTTAGG GGTTCCACGA
ATACGGCAAC TCCGAGTCAG AAATGGATCC TGCTCTATCC CCCAGGACTT GAGAGATGAA
ATTAAAGAGT GCTATGATGT CTACTCTGTC AGTAGTGAAG ATAGGGCTCC CTTTGGGCCC
CGAAATGGAA CCGCTTGGAT CTACACAAGT GAAAAAGACT TGAATGGTAG TAGCCACTGG
GGAATCATTG CAACTTATAG TGGAGCTGGC TATTATCTGG ATTTGTCAAG AACAAGAGAG
GAAACAGCTG CACAAGTTGC TAGCCTCAAG AAAAATGTCT GGCTGGACCG AGGAACCAGG
GCAACTTTTA TTGACTTCTC AGTGTACAAC GCCAACATTA ACCTGTTCTG TGTGGTCAGG
TTATTGGTTG AATTCCCAGC AACAGGTGGT GTGATTCCAT CTTGGCAATT TCAGCCTTTA
AAGCTGATCC GATATGTCAC AACTTTTGAT TTCTTCCTGG CAGCCTGTGA GATTATCTTT
TGTTTCTTTA TCTTTTACTA TGTGGTGGAA GAGATATTGG AAATTCGCAT TCACAAACTA
CACTATTTCA GGAGTTTCTG GAATTGTCTG GATGTTGTGA TCGTTGTGCT GTCAGTGGTA
GCTATAGGAA TTAACATATA CAGAACATCA AATGTGGAGG TGCTACTACA GTTTCTGGAA
GATCAAAATA CTTTCCCCAA CTTTGAGCAT CTGGCATATT GGCAGATACA GTTCAACAAT
ATAGCTGCTG TCACAGTATT TTTTGTCTGG ATTAAGCTCT TCAAATTCAT CAATTTTAAC
AGGACCATGA GCCAGCTCTC GACAACCATG TCTCGATGTG CCAAAGACCT GTTTGGCTTT
GCTATTATGT TCTTCATTAT TTTCCTAGCG TATGCTCAGT TGGCATACCT TGTCTTTGGC
ACTCAGGTCG ATGACTTCAG TACTTTCCAA GAGTGTATCT TCACTCAATT CCGTATCATT
TTGGGCGATA TCAACTTTGC AGAGATTGAG GAAGCTAATC GAGTTTTGGG ACCAATTTAT
TTCACTACAT TTGTGTTCTT TATGTTCTTC ATTCTTTTGA ATATGTTTTT GGCTATCATC
AATGATACTT ACTCTGAAGT GAAATCTGAC TTGGCACAGC AGAAAGCTGA AATGGAACTC
TCAGATCTTA TCAGAAAGGG CTACCATAAA GCTTTGGTCA AACTAAAACT GAAAAAAAAT
ACCGTGGATG ACATTTCAGA GAGTCTGCGG CAAGGAGGAG GCAAGTTAAA CTTTGACGAA
CTTCGACAAG ATCTCAAAGG GAAGGGCCAT ACTGATGCAG AGATTGAGGC AATATTCACA
AAGTACGACC AAGATGGAGA CCAAGAACTG ACCGAACATG AACATCAGCA GATGAGAGAC
GACTTGGAGA AAGAGAGGGA GGACCTGGAT TTGGATCACA GTTCTTTACC ACGTCCCATG
AGCAGCCGAA GTTTCCCTCG AAGCCTGGAT GACTCTGAGG AGGATGACGA TGAAGATAGC
GGACATAGCT CCAGAAGGAG GGGAAGCATT TCTAGTGGCG TTTCTTACGA AGAGTTTCAA
GTCCTGGTGA GACGAGTGGA CCGGATGGAG CATTCCATCG GCAGCATAGT GTCCAAGATT
GACGCCGTGA TCGTGAAGCT AGAGATTATG GAGCGAGCCA AACTGAAGAG GAGGGAGGTG
CTGGGAAGGC TGTTGGATGG GGTGGCCGAG GATGAAAGGC TGGGTCGTGA CAGTGAAATC
CATAGGGAAC AGATGGAACG GCTAGTACGT GAAGAGTTGG AACGCTGGGA ATCCGATGAT
GCAGCTTCCC AGATCAGTCA TGGTTTAGGC ACGCCAGTGG GACTAAATGG TCAACCTCGC
CCCAGAAGCT CCCGCCCATC TTCCTCCCAA TCTACAGAAG GCATGGAAGG TGCAGGTGGA
AATGGGAGTT CTAATGTCCA CGTATGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_000288.1
CDS88..2994
Misc Feature(1)28..30(+)
Misc Feature(2)583..585(+)
Misc Feature(3)757..819(+)
Misc Feature(4)886..2148(+)
Misc Feature(5)1033..1071(+)
Misc Feature(6)1492..1554(+)
Misc Feature(7)1603..1665(+)
Misc Feature(8)1738..1800(+)
Misc Feature(9)1882..1944(+)
Misc Feature(10)2062..2124(+)
Misc Feature(11)2197..2469(+)
Misc Feature(12)2281..2421(+)
Misc Feature(13)2284..2409(+)
Misc Feature(14)2494..2553(+)
Misc Feature(15)2521..2523(+)
Misc Feature(16)2569..2991(+)
Exon (1)1..682
Gene:PKD2
Gene Synonym:
Exon (2)683..796
Gene:PKD2
Gene Synonym:
Exon (3)797..930
Gene:PKD2
Gene Synonym:
Exon (4)931..1181
Gene:PKD2
Gene Synonym:
Exon (5)1182..1406
Gene:PKD2
Gene Synonym:
Exon (6)1407..1635
Gene:PKD2
Gene Synonym:
Exon (7)1636..1803
Gene:PKD2
Gene Synonym:
Exon (8)1804..1985
Gene:PKD2
Gene Synonym:
Exon (9)1986..2106
Gene:PKD2
Gene Synonym:
Exon (10)2107..2205
Gene:PKD2
Gene Synonym:
Exon (11)2206..2327
Gene:PKD2
Gene Synonym:
Exon (12)2328..2445
Gene:PKD2
Gene Synonym:
Exon (13)2446..2609
Gene:PKD2
Gene Synonym:
Exon (14)2610..2757
Gene:PKD2
Gene Synonym:
Exon (15)2758..5079
Gene:PKD2
Gene Synonym:
Translation

Target ORF information:

RefSeq Version NM_000297
Organism Homo sapiens (human)
Definition Homo sapiens polycystic kidney disease 2 (autosomal dominant) (PKD2), mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_000297

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
ATGGTGAACT CCAGTCGCGT GCAGCCTCAG CAGCCCGGGG ACGCCAAGCG GCCGCCCGCG 
CCCCGCGCGC CGGACCCGGG CCGGCTGATG GCTGGCTGCG CGGCCGTGGG CGCCAGCCTC
GCCGCCCCGG GCGGCCTCTG CGAGCAGCGG GGCCTGGAGA TCGAGATGCA GCGCATCCGG
CAGGCGGCCG CGCGGGACCC CCCGGCCGGA GCCGCGGCCT CCCCTTCTCC TCCGCTCTCG
TCGTGCTCCC GGCAGGCGTG GAGCCGCGAT AACCCCGGCT TCGAGGCCGA GGAGGAGGAG
GAGGAGGTGG AAGGGGAAGA AGGCGGAATG GTGGTGGAGA TGGACGTAGA GTGGCGCCCG
GGCAGCCGGA GGTCGGCCGC CTCCTCGGCC GTGAGCTCCG TGGGCGCGCG GAGCCGGGGG
CTTGGGGGCT ACCACGGCGC GGGCCACCCG AGCGGGAGGC GGCGCCGGCG AGAGGACCAG
GGCCCGCCGT GCCCCAGCCC AGTCGGCGGC GGGGACCCGC TGCATCGCCA CCTCCCCCTG
GAAGGGCAGC CGCCCCGAGT GGCCTGGGCG GAGAGGCTGG TTCGCGGGCT GCGAGGTCTC
TGGGGAACAA GACTCATGGA GGAAAGCAGC ACTAACCGAG AGAAATACCT TAAAAGTGTT
TTACGGGAAC TGGTCACATA CCTCCTTTTT CTCATAGTCT TGTGCATCTT GACCTACGGC
ATGATGAGCT CCAATGTGTA CTACTACACC CGGATGATGT CACAGCTCTT CCTAGACACC
CCCGTGTCCA AAACGGAGAA AACTAACTTT AAAACTCTGT CTTCCATGGA AGACTTCTGG
AAGTTCACAG AAGGCTCCTT ATTGGATGGG CTGTACTGGA AGATGCAGCC CAGCAACCAG
ACTGAAGCTG ACAACCGAAG TTTCATCTTC TATGAGAACC TGCTGTTAGG GGTTCCACGA
ATACGGCAAC TCCGAGTCAG AAATGGATCC TGCTCTATCC CCCAGGACTT GAGAGATGAA
ATTAAAGAGT GCTATGATGT CTACTCTGTC AGTAGTGAAG ATAGGGCTCC CTTTGGGCCC
CGAAATGGAA CCGCTTGGAT CTACACAAGT GAAAAAGACT TGAATGGTAG TAGCCACTGG
GGAATCATTG CAACTTATAG TGGAGCTGGC TATTATCTGG ATTTGTCAAG AACAAGAGAG
GAAACAGCTG CACAAGTTGC TAGCCTCAAG AAAAATGTCT GGCTGGACCG AGGAACCAGG
GCAACTTTTA TTGACTTCTC AGTGTACAAC GCCAACATTA ACCTGTTCTG TGTGGTCAGG
TTATTGGTTG AATTCCCAGC AACAGGTGGT GTGATTCCAT CTTGGCAATT TCAGCCTTTA
AAGCTGATCC GATATGTCAC AACTTTTGAT TTCTTCCTGG CAGCCTGTGA GATTATCTTT
TGTTTCTTTA TCTTTTACTA TGTGGTGGAA GAGATATTGG AAATTCGCAT TCACAAACTA
CACTATTTCA GGAGTTTCTG GAATTGTCTG GATGTTGTGA TCGTTGTGCT GTCAGTGGTA
GCTATAGGAA TTAACATATA CAGAACATCA AATGTGGAGG TGCTACTACA GTTTCTGGAA
GATCAAAATA CTTTCCCCAA CTTTGAGCAT CTGGCATATT GGCAGATACA GTTCAACAAT
ATAGCTGCTG TCACAGTATT TTTTGTCTGG ATTAAGCTCT TCAAATTCAT CAATTTTAAC
AGGACCATGA GCCAGCTCTC GACAACCATG TCTCGATGTG CCAAAGACCT GTTTGGCTTT
GCTATTATGT TCTTCATTAT TTTCCTAGCG TATGCTCAGT TGGCATACCT TGTCTTTGGC
ACTCAGGTCG ATGACTTCAG TACTTTCCAA GAGTGTATCT TCACTCAATT CCGTATCATT
TTGGGCGATA TCAACTTTGC AGAGATTGAG GAAGCTAATC GAGTTTTGGG ACCAATTTAT
TTCACTACAT TTGTGTTCTT TATGTTCTTC ATTCTTTTGA ATATGTTTTT GGCTATCATC
AATGATACTT ACTCTGAAGT GAAATCTGAC TTGGCACAGC AGAAAGCTGA AATGGAACTC
TCAGATCTTA TCAGAAAGGG CTACCATAAA GCTTTGGTCA AACTAAAACT GAAAAAAAAT
ACCGTGGATG ACATTTCAGA GAGTCTGCGG CAAGGAGGAG GCAAGTTAAA CTTTGACGAA
CTTCGACAAG ATCTCAAAGG GAAGGGCCAT ACTGATGCAG AGATTGAGGC AATATTCACA
AAGTACGACC AAGATGGAGA CCAAGAACTG ACCGAACATG AACATCAGCA GATGAGAGAC
GACTTGGAGA AAGAGAGGGA GGACCTGGAT TTGGATCACA GTTCTTTACC ACGTCCCATG
AGCAGCCGAA GTTTCCCTCG AAGCCTGGAT GACTCTGAGG AGGATGACGA TGAAGATAGC
GGACATAGCT CCAGAAGGAG GGGAAGCATT TCTAGTGGCG TTTCTTACGA AGAGTTTCAA
GTCCTGGTGA GACGAGTGGA CCGGATGGAG CATTCCATCG GCAGCATAGT GTCCAAGATT
GACGCCGTGA TCGTGAAGCT AGAGATTATG GAGCGAGCCA AACTGAAGAG GAGGGAGGTG
CTGGGAAGGC TGTTGGATGG GGTGGCCGAG GATGAAAGGC TGGGTCGTGA CAGTGAAATC
CATAGGGAAC AGATGGAACG GCTAGTACGT GAAGAGTTGG AACGCTGGGA ATCCGATGAT
GCAGCTTCCC AGATCAGTCA TGGTTTAGGC ACGCCAGTGG GACTAAATGG TCAACCTCGC
CCCAGAAGCT CCCGCCCATC TTCCTCCCAA TCTACAGAAG GCATGGAAGG TGCAGGTGGA
AATGGGAGTT CTAATGTCCA CGTATGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu59727
Clone ID Related Accession (Same CDS sequence) XM_011532028
Accession Version XM_011532028.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 2682bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product polycystin-2 isoform X1
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_016354.20) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
ATGGTGAACT CCAGTCGCGT GCAGCCTCAG CAGCCCGGGG ACGCCAAGCG GCCGCCCGCG 
CCCCGCGCGC CGGACCCGGG CCGGCTGATG GCTGGCTGCG CGGCCGTGGG CGCCAGCCTC
GCCGCCCCGG GCGGCCTCTG CGAGCAGCGG GGCCTGGAGA TCGAGATGCA GCGCATCCGG
CAGGCGGCCG CGCGGGACCC CCCGGCCGGA GCCGCGGCCT CCCCTTCTCC TCCGCTCTCG
TCGTGCTCCC GGCAGGCGTG GAGCCGCGAT AACCCCGGCT TCGAGGCCGA GGAGGAGGAG
GAGGAGGTGG AAGGGGAAGA AGGCGGAATG GTGGTGGAGA TGGACGTAGA GTGGCGCCCG
GGCAGCCGGA GGTCGGCCGC CTCCTCGGCC GTGAGCTCCG TGGGCGCGCG GAGCCGGGGG
CTTGGGGGCT ACCACGGCGC GGGCCACCCG AGCGGGAGGC GGCGCCGGCG AGAGGACCAG
GGCCCGCCGT GCCCCAGCCC AGTCGGCGGC GGGGACCCGC TGCATCGCCA CCTCCCCCTG
GAAGGGCAGC CGCCCCGAGT GGCCTGGGCG GAGAGGCTGG TTCGCGGGCT GCGAGGTCTC
TGGGGAACAA GACTCATGGA GGAAAGCAGC ACTAACCGAG AGAAATACCT TAAAAGTGTT
TTACGGGAAC TGGTCACATA CCTCCTTTTT CTCATAGTCT TGTGCATCTT GACCTACGGC
ATGATGAGCT CCAATGTGTA CTACTACACC CGGATGATGT CACAGCTCTT CCTAGACACC
CCCGTGTCCA AAACGGAGAA AACTAACTTT AAAACTCTGT CTTCCATGGA AGACTTCTGG
AAGTTCACAG AAGGCTCCTT ATTGGATGGG CTGTACTGGA AGATGCAGCC CAGCAACCAG
ACTGAAGCTG ACAACCGAAG TTTCATCTTC TATGAGAACC TGCTGTTAGG GGTTCCACGA
ATACGGCAAC TCCGAGTCAG AAATGGATCC TGCTCTATCC CCCAGGACTT GAGAGATGAA
ATTAAAGAGT GCTATGATGT CTACTCTGTC AGTAGTGAAG ATAGGGCTCC CTTTGGGCCC
CGAAATGGAA CCGCGTTATT GGTTGAATTC CCAGCAACAG GTGGTGTGAT TCCATCTTGG
CAATTTCAGC CTTTAAAGCT GATCCGATAT GTCACAACTT TTGATTTCTT CCTGGCAGCC
TGTGAGATTA TCTTTTGTTT CTTTATCTTT TACTATGTGG TGGAAGAGAT ATTGGAAATT
CGCATTCACA AACTACACTA TTTCAGGAGT TTCTGGAATT GTCTGGATGT TGTGATCGTT
GTGCTGTCAG TGGTAGCTAT AGGAATTAAC ATATACAGAA CATCAAATGT GGAGGTGCTA
CTACAGTTTC TGGAAGATCA AAATACTTTC CCCAACTTTG AGCATCTGGC ATATTGGCAG
ATACAGTTCA ACAATATAGC TGCTGTCACA GTATTTTTTG TCTGGATTAA GCTCTTCAAA
TTCATCAATT TTAACAGGAC CATGAGCCAG CTCTCGACAA CCATGTCTCG ATGTGCCAAA
GACCTGTTTG GCTTTGCTAT TATGTTCTTC ATTATTTTCC TAGCGTATGC TCAGTTGGCA
TACCTTGTCT TTGGCACTCA GGTCGATGAC TTCAGTACTT TCCAAGAGTG TATCTTCACT
CAATTCCGTA TCATTTTGGG CGATATCAAC TTTGCAGAGA TTGAGGAAGC TAATCGAGTT
TTGGGACCAA TTTATTTCAC TACATTTGTG TTCTTTATGT TCTTCATTCT TTTGAATATG
TTTTTGGCTA TCATCAATGA TACTTACTCT GAAGTGAAAT CTGACTTGGC ACAGCAGAAA
GCTGAAATGG AACTCTCAGA TCTTATCAGA AAGGGCTACC ATAAAGCTTT GGTCAAACTA
AAACTGAAAA AAAATACCGT GGATGACATT TCAGAGAGTC TGCGGCAAGG AGGAGGCAAG
TTAAACTTTG ACGAACTTCG ACAAGATCTC AAAGGGAAGG GCCATACTGA TGCAGAGATT
GAGGCAATAT TCACAAAGTA CGACCAAGAT GGAGACCAAG AACTGACCGA ACATGAACAT
CAGCAGATGA GAGACGACTT GGAGAAAGAG AGGGAGGACC TGGATTTGGA TCACAGTTCT
TTACCACGTC CCATGAGCAG CCGAAGTTTC CCTCGAAGCC TGGATGACTC TGAGGAGGAT
GACGATGAAG ATAGCGGACA TAGCTCCAGA AGGAGGGGAA GCATTTCTAG TGGCGTTTCT
TACGAAGAGT TTCAAGTCCT GGTGAGACGA GTGGACCGGA TGGAGCATTC CATCGGCAGC
ATAGTGTCCA AGATTGACGC CGTGATCGTG AAGCTAGAGA TTATGGAGCG AGCCAAACTG
AAGAGGAGGG AGGTGCTGGG AAGGCTGTTG GATGGGGTGG CCGAGGATGA AAGGCTGGGT
CGTGACAGTG AAATCCATAG GGAACAGATG GAACGGCTAG TACGTGAAGA GTTGGAACGC
TGGGAATCCG ATGATGCAGC TTCCCAGATC AGTCATGGTT TAGGCACGCC AGTGGGACTA
AATGGTCAAC CTCGCCCCAG AAGCTCCCGC CCATCTTCCT CCCAATCTAC AGAAGGCATG
GAAGGTGCAG GTGGAAATGG GAGTTCTAAT GTCCACGTAT GA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_011530330.1
CDS90..2771
Misc Feature(1)888..1925(+)
Misc Feature(2)2058..2198(+)
Misc Feature(3)2061..2186(+)
Translation

Target ORF information:

RefSeq Version XM_011532028
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens polycystic kidney disease 2 (autosomal dominant) (PKD2), transcript variant X1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_011532028

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
ATGGTGAACT CCAGTCGCGT GCAGCCTCAG CAGCCCGGGG ACGCCAAGCG GCCGCCCGCG 
CCCCGCGCGC CGGACCCGGG CCGGCTGATG GCTGGCTGCG CGGCCGTGGG CGCCAGCCTC
GCCGCCCCGG GCGGCCTCTG CGAGCAGCGG GGCCTGGAGA TCGAGATGCA GCGCATCCGG
CAGGCGGCCG CGCGGGACCC CCCGGCCGGA GCCGCGGCCT CCCCTTCTCC TCCGCTCTCG
TCGTGCTCCC GGCAGGCGTG GAGCCGCGAT AACCCCGGCT TCGAGGCCGA GGAGGAGGAG
GAGGAGGTGG AAGGGGAAGA AGGCGGAATG GTGGTGGAGA TGGACGTAGA GTGGCGCCCG
GGCAGCCGGA GGTCGGCCGC CTCCTCGGCC GTGAGCTCCG TGGGCGCGCG GAGCCGGGGG
CTTGGGGGCT ACCACGGCGC GGGCCACCCG AGCGGGAGGC GGCGCCGGCG AGAGGACCAG
GGCCCGCCGT GCCCCAGCCC AGTCGGCGGC GGGGACCCGC TGCATCGCCA CCTCCCCCTG
GAAGGGCAGC CGCCCCGAGT GGCCTGGGCG GAGAGGCTGG TTCGCGGGCT GCGAGGTCTC
TGGGGAACAA GACTCATGGA GGAAAGCAGC ACTAACCGAG AGAAATACCT TAAAAGTGTT
TTACGGGAAC TGGTCACATA CCTCCTTTTT CTCATAGTCT TGTGCATCTT GACCTACGGC
ATGATGAGCT CCAATGTGTA CTACTACACC CGGATGATGT CACAGCTCTT CCTAGACACC
CCCGTGTCCA AAACGGAGAA AACTAACTTT AAAACTCTGT CTTCCATGGA AGACTTCTGG
AAGTTCACAG AAGGCTCCTT ATTGGATGGG CTGTACTGGA AGATGCAGCC CAGCAACCAG
ACTGAAGCTG ACAACCGAAG TTTCATCTTC TATGAGAACC TGCTGTTAGG GGTTCCACGA
ATACGGCAAC TCCGAGTCAG AAATGGATCC TGCTCTATCC CCCAGGACTT GAGAGATGAA
ATTAAAGAGT GCTATGATGT CTACTCTGTC AGTAGTGAAG ATAGGGCTCC CTTTGGGCCC
CGAAATGGAA CCGCGTTATT GGTTGAATTC CCAGCAACAG GTGGTGTGAT TCCATCTTGG
CAATTTCAGC CTTTAAAGCT GATCCGATAT GTCACAACTT TTGATTTCTT CCTGGCAGCC
TGTGAGATTA TCTTTTGTTT CTTTATCTTT TACTATGTGG TGGAAGAGAT ATTGGAAATT
CGCATTCACA AACTACACTA TTTCAGGAGT TTCTGGAATT GTCTGGATGT TGTGATCGTT
GTGCTGTCAG TGGTAGCTAT AGGAATTAAC ATATACAGAA CATCAAATGT GGAGGTGCTA
CTACAGTTTC TGGAAGATCA AAATACTTTC CCCAACTTTG AGCATCTGGC ATATTGGCAG
ATACAGTTCA ACAATATAGC TGCTGTCACA GTATTTTTTG TCTGGATTAA GCTCTTCAAA
TTCATCAATT TTAACAGGAC CATGAGCCAG CTCTCGACAA CCATGTCTCG ATGTGCCAAA
GACCTGTTTG GCTTTGCTAT TATGTTCTTC ATTATTTTCC TAGCGTATGC TCAGTTGGCA
TACCTTGTCT TTGGCACTCA GGTCGATGAC TTCAGTACTT TCCAAGAGTG TATCTTCACT
CAATTCCGTA TCATTTTGGG CGATATCAAC TTTGCAGAGA TTGAGGAAGC TAATCGAGTT
TTGGGACCAA TTTATTTCAC TACATTTGTG TTCTTTATGT TCTTCATTCT TTTGAATATG
TTTTTGGCTA TCATCAATGA TACTTACTCT GAAGTGAAAT CTGACTTGGC ACAGCAGAAA
GCTGAAATGG AACTCTCAGA TCTTATCAGA AAGGGCTACC ATAAAGCTTT GGTCAAACTA
AAACTGAAAA AAAATACCGT GGATGACATT TCAGAGAGTC TGCGGCAAGG AGGAGGCAAG
TTAAACTTTG ACGAACTTCG ACAAGATCTC AAAGGGAAGG GCCATACTGA TGCAGAGATT
GAGGCAATAT TCACAAAGTA CGACCAAGAT GGAGACCAAG AACTGACCGA ACATGAACAT
CAGCAGATGA GAGACGACTT GGAGAAAGAG AGGGAGGACC TGGATTTGGA TCACAGTTCT
TTACCACGTC CCATGAGCAG CCGAAGTTTC CCTCGAAGCC TGGATGACTC TGAGGAGGAT
GACGATGAAG ATAGCGGACA TAGCTCCAGA AGGAGGGGAA GCATTTCTAG TGGCGTTTCT
TACGAAGAGT TTCAAGTCCT GGTGAGACGA GTGGACCGGA TGGAGCATTC CATCGGCAGC
ATAGTGTCCA AGATTGACGC CGTGATCGTG AAGCTAGAGA TTATGGAGCG AGCCAAACTG
AAGAGGAGGG AGGTGCTGGG AAGGCTGTTG GATGGGGTGG CCGAGGATGA AAGGCTGGGT
CGTGACAGTG AAATCCATAG GGAACAGATG GAACGGCTAG TACGTGAAGA GTTGGAACGC
TGGGAATCCG ATGATGCAGC TTCCCAGATC AGTCATGGTT TAGGCACGCC AGTGGGACTA
AATGGTCAAC CTCGCCCCAG AAGCTCCCGC CCATCTTCCT CCCAATCTAC AGAAGGCATG
GAAGGTGCAG GTGGAAATGG GAGTTCTAAT GTCCACGTAT GA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu59728
Clone ID Related Accession (Same CDS sequence) XM_011532029
Accession Version XM_011532029.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 2187bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product polycystin-2 isoform X2
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_016354.20) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
ATGATGAGCT CCAATGTGTA CTACTACACC CGGATGATGT CACAGCTCTT CCTAGACACC 
CCCGTGTCCA AAACGGAGAA AACTAACTTT AAAACTCTGT CTTCCATGGA AGACTTCTGG
AAGTTCACAG AAGGCTCCTT ATTGGATGGG CTGTACTGGA AGATGCAGCC CAGCAACCAG
ACTGAAGCTG ACAACCGAAG TTTCATCTTC TATGAGAACC TGCTGTTAGG GGTTCCACGA
ATACGGCAAC TCCGAGTCAG AAATGGATCC TGCTCTATCC CCCAGGACTT GAGAGATGAA
ATTAAAGAGT GCTATGATGT CTACTCTGTC AGTAGTGAAG ATAGGGCTCC CTTTGGGCCC
CGAAATGGAA CCGCTTGGAT CTACACAAGT GAAAAAGACT TGAATGGTAG TAGCCACTGG
GGAATCATTG CAACTTATAG TGGAGCTGGC TATTATCTGG ATTTGTCAAG AACAAGAGAG
GAAACAGCTG CACAAGTTGC TAGCCTCAAG AAAAATGTCT GGCTGGACCG AGGAACCAGG
GCAACTTTTA TTGACTTCTC AGTGTACAAC GCCAACATTA ACCTGTTCTG TGTGGTCAGG
TTATTGGTTG AATTCCCAGC AACAGGTGGT GTGATTCCAT CTTGGCAATT TCAGCCTTTA
AAGCTGATCC GATATGTCAC AACTTTTGAT TTCTTCCTGG CAGCCTGTGA GATTATCTTT
TGTTTCTTTA TCTTTTACTA TGTGGTGGAA GAGATATTGG AAATTCGCAT TCACAAACTA
CACTATTTCA GGAGTTTCTG GAATTGTCTG GATGTTGTGA TCGTTGTGCT GTCAGTGGTA
GCTATAGGAA TTAACATATA CAGAACATCA AATGTGGAGG TGCTACTACA GTTTCTGGAA
GATCAAAATA CTTTCCCCAA CTTTGAGCAT CTGGCATATT GGCAGATACA GTTCAACAAT
ATAGCTGCTG TCACAGTATT TTTTGTCTGG ATTAAGCTCT TCAAATTCAT CAATTTTAAC
AGGACCATGA GCCAGCTCTC GACAACCATG TCTCGATGTG CCAAAGACCT GTTTGGCTTT
GCTATTATGT TCTTCATTAT TTTCCTAGCG TATGCTCAGT TGGCATACCT TGTCTTTGGC
ACTCAGGTCG ATGACTTCAG TACTTTCCAA GAGTGTATCT TCACTCAATT CCGTATCATT
TTGGGCGATA TCAACTTTGC AGAGATTGAG GAAGCTAATC GAGTTTTGGG ACCAATTTAT
TTCACTACAT TTGTGTTCTT TATGTTCTTC ATTCTTTTGA ATATGTTTTT GGCTATCATC
AATGATACTT ACTCTGAAGT GAAATCTGAC TTGGCACAGC AGAAAGCTGA AATGGAACTC
TCAGATCTTA TCAGAAAGGG CTACCATAAA GCTTTGGTCA AACTAAAACT GAAAAAAAAT
ACCGTGGATG ACATTTCAGA GAGTCTGCGG CAAGGAGGAG GCAAGTTAAA CTTTGACGAA
CTTCGACAAG ATCTCAAAGG GAAGGGCCAT ACTGATGCAG AGATTGAGGC AATATTCACA
AAGTACGACC AAGATGGAGA CCAAGAACTG ACCGAACATG AACATCAGCA GATGAGAGAC
GACTTGGAGA AAGAGAGGGA GGACCTGGAT TTGGATCACA GTTCTTTACC ACGTCCCATG
AGCAGCCGAA GTTTCCCTCG AAGCCTGGAT GACTCTGAGG AGGATGACGA TGAAGATAGC
GGACATAGCT CCAGAAGGAG GGGAAGCATT TCTAGTGGCG TTTCTTACGA AGAGTTTCAA
GTCCTGGTGA GACGAGTGGA CCGGATGGAG CATTCCATCG GCAGCATAGT GTCCAAGATT
GACGCCGTGA TCGTGAAGCT AGAGATTATG GAGCGAGCCA AACTGAAGAG GAGGGAGGTG
CTGGGAAGGC TGTTGGATGG GGTGGCCGAG GATGAAAGGC TGGGTCGTGA CAGTGAAATC
CATAGGGAAC AGATGGAACG GCTAGTACGT GAAGAGTTGG AACGCTGGGA ATCCGATGAT
GCAGCTTCCC AGATCAGTCA TGGTTTAGGC ACGCCAGTGG GACTAAATGG TCAACCTCGC
CCCAGAAGCT CCCGCCCATC TTCCTCCCAA TCTACAGAAG GCATGGAAGG TGCAGGTGGA
AATGGGAGTT CTAATGTCCA CGTATGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_011530331.1
CDS98..2284
Misc Feature(1)176..1438(+)
Misc Feature(2)1571..1711(+)
Misc Feature(3)1574..1699(+)
Translation

Target ORF information:

RefSeq Version XM_011532029
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens polycystic kidney disease 2 (autosomal dominant) (PKD2), transcript variant X2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_011532029

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
ATGATGAGCT CCAATGTGTA CTACTACACC CGGATGATGT CACAGCTCTT CCTAGACACC 
CCCGTGTCCA AAACGGAGAA AACTAACTTT AAAACTCTGT CTTCCATGGA AGACTTCTGG
AAGTTCACAG AAGGCTCCTT ATTGGATGGG CTGTACTGGA AGATGCAGCC CAGCAACCAG
ACTGAAGCTG ACAACCGAAG TTTCATCTTC TATGAGAACC TGCTGTTAGG GGTTCCACGA
ATACGGCAAC TCCGAGTCAG AAATGGATCC TGCTCTATCC CCCAGGACTT GAGAGATGAA
ATTAAAGAGT GCTATGATGT CTACTCTGTC AGTAGTGAAG ATAGGGCTCC CTTTGGGCCC
CGAAATGGAA CCGCTTGGAT CTACACAAGT GAAAAAGACT TGAATGGTAG TAGCCACTGG
GGAATCATTG CAACTTATAG TGGAGCTGGC TATTATCTGG ATTTGTCAAG AACAAGAGAG
GAAACAGCTG CACAAGTTGC TAGCCTCAAG AAAAATGTCT GGCTGGACCG AGGAACCAGG
GCAACTTTTA TTGACTTCTC AGTGTACAAC GCCAACATTA ACCTGTTCTG TGTGGTCAGG
TTATTGGTTG AATTCCCAGC AACAGGTGGT GTGATTCCAT CTTGGCAATT TCAGCCTTTA
AAGCTGATCC GATATGTCAC AACTTTTGAT TTCTTCCTGG CAGCCTGTGA GATTATCTTT
TGTTTCTTTA TCTTTTACTA TGTGGTGGAA GAGATATTGG AAATTCGCAT TCACAAACTA
CACTATTTCA GGAGTTTCTG GAATTGTCTG GATGTTGTGA TCGTTGTGCT GTCAGTGGTA
GCTATAGGAA TTAACATATA CAGAACATCA AATGTGGAGG TGCTACTACA GTTTCTGGAA
GATCAAAATA CTTTCCCCAA CTTTGAGCAT CTGGCATATT GGCAGATACA GTTCAACAAT
ATAGCTGCTG TCACAGTATT TTTTGTCTGG ATTAAGCTCT TCAAATTCAT CAATTTTAAC
AGGACCATGA GCCAGCTCTC GACAACCATG TCTCGATGTG CCAAAGACCT GTTTGGCTTT
GCTATTATGT TCTTCATTAT TTTCCTAGCG TATGCTCAGT TGGCATACCT TGTCTTTGGC
ACTCAGGTCG ATGACTTCAG TACTTTCCAA GAGTGTATCT TCACTCAATT CCGTATCATT
TTGGGCGATA TCAACTTTGC AGAGATTGAG GAAGCTAATC GAGTTTTGGG ACCAATTTAT
TTCACTACAT TTGTGTTCTT TATGTTCTTC ATTCTTTTGA ATATGTTTTT GGCTATCATC
AATGATACTT ACTCTGAAGT GAAATCTGAC TTGGCACAGC AGAAAGCTGA AATGGAACTC
TCAGATCTTA TCAGAAAGGG CTACCATAAA GCTTTGGTCA AACTAAAACT GAAAAAAAAT
ACCGTGGATG ACATTTCAGA GAGTCTGCGG CAAGGAGGAG GCAAGTTAAA CTTTGACGAA
CTTCGACAAG ATCTCAAAGG GAAGGGCCAT ACTGATGCAG AGATTGAGGC AATATTCACA
AAGTACGACC AAGATGGAGA CCAAGAACTG ACCGAACATG AACATCAGCA GATGAGAGAC
GACTTGGAGA AAGAGAGGGA GGACCTGGAT TTGGATCACA GTTCTTTACC ACGTCCCATG
AGCAGCCGAA GTTTCCCTCG AAGCCTGGAT GACTCTGAGG AGGATGACGA TGAAGATAGC
GGACATAGCT CCAGAAGGAG GGGAAGCATT TCTAGTGGCG TTTCTTACGA AGAGTTTCAA
GTCCTGGTGA GACGAGTGGA CCGGATGGAG CATTCCATCG GCAGCATAGT GTCCAAGATT
GACGCCGTGA TCGTGAAGCT AGAGATTATG GAGCGAGCCA AACTGAAGAG GAGGGAGGTG
CTGGGAAGGC TGTTGGATGG GGTGGCCGAG GATGAAAGGC TGGGTCGTGA CAGTGAAATC
CATAGGGAAC AGATGGAACG GCTAGTACGT GAAGAGTTGG AACGCTGGGA ATCCGATGAT
GCAGCTTCCC AGATCAGTCA TGGTTTAGGC ACGCCAGTGG GACTAAATGG TCAACCTCGC
CCCAGAAGCT CCCGCCCATC TTCCTCCCAA TCTACAGAAG GCATGGAAGG TGCAGGTGGA
AATGGGAGTT CTAATGTCCA CGTATGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu59729
Clone ID Related Accession (Same CDS sequence) XM_011532030
Accession Version XM_011532030.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 2067bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product polycystin-2 isoform X3
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_016354.20) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
ATGTTCACAG AAGGCTCCTT ATTGGATGGG CTGTACTGGA AGATGCAGCC CAGCAACCAG 
ACTGAAGCTG ACAACCGAAG TTTCATCTTC TATGAGAACC TGCTGTTAGG GGTTCCACGA
ATACGGCAAC TCCGAGTCAG AAATGGATCC TGCTCTATCC CCCAGGACTT GAGAGATGAA
ATTAAAGAGT GCTATGATGT CTACTCTGTC AGTAGTGAAG ATAGGGCTCC CTTTGGGCCC
CGAAATGGAA CCGCTTGGAT CTACACAAGT GAAAAAGACT TGAATGGTAG TAGCCACTGG
GGAATCATTG CAACTTATAG TGGAGCTGGC TATTATCTGG ATTTGTCAAG AACAAGAGAG
GAAACAGCTG CACAAGTTGC TAGCCTCAAG AAAAATGTCT GGCTGGACCG AGGAACCAGG
GCAACTTTTA TTGACTTCTC AGTGTACAAC GCCAACATTA ACCTGTTCTG TGTGGTCAGG
TTATTGGTTG AATTCCCAGC AACAGGTGGT GTGATTCCAT CTTGGCAATT TCAGCCTTTA
AAGCTGATCC GATATGTCAC AACTTTTGAT TTCTTCCTGG CAGCCTGTGA GATTATCTTT
TGTTTCTTTA TCTTTTACTA TGTGGTGGAA GAGATATTGG AAATTCGCAT TCACAAACTA
CACTATTTCA GGAGTTTCTG GAATTGTCTG GATGTTGTGA TCGTTGTGCT GTCAGTGGTA
GCTATAGGAA TTAACATATA CAGAACATCA AATGTGGAGG TGCTACTACA GTTTCTGGAA
GATCAAAATA CTTTCCCCAA CTTTGAGCAT CTGGCATATT GGCAGATACA GTTCAACAAT
ATAGCTGCTG TCACAGTATT TTTTGTCTGG ATTAAGCTCT TCAAATTCAT CAATTTTAAC
AGGACCATGA GCCAGCTCTC GACAACCATG TCTCGATGTG CCAAAGACCT GTTTGGCTTT
GCTATTATGT TCTTCATTAT TTTCCTAGCG TATGCTCAGT TGGCATACCT TGTCTTTGGC
ACTCAGGTCG ATGACTTCAG TACTTTCCAA GAGTGTATCT TCACTCAATT CCGTATCATT
TTGGGCGATA TCAACTTTGC AGAGATTGAG GAAGCTAATC GAGTTTTGGG ACCAATTTAT
TTCACTACAT TTGTGTTCTT TATGTTCTTC ATTCTTTTGA ATATGTTTTT GGCTATCATC
AATGATACTT ACTCTGAAGT GAAATCTGAC TTGGCACAGC AGAAAGCTGA AATGGAACTC
TCAGATCTTA TCAGAAAGGG CTACCATAAA GCTTTGGTCA AACTAAAACT GAAAAAAAAT
ACCGTGGATG ACATTTCAGA GAGTCTGCGG CAAGGAGGAG GCAAGTTAAA CTTTGACGAA
CTTCGACAAG ATCTCAAAGG GAAGGGCCAT ACTGATGCAG AGATTGAGGC AATATTCACA
AAGTACGACC AAGATGGAGA CCAAGAACTG ACCGAACATG AACATCAGCA GATGAGAGAC
GACTTGGAGA AAGAGAGGGA GGACCTGGAT TTGGATCACA GTTCTTTACC ACGTCCCATG
AGCAGCCGAA GTTTCCCTCG AAGCCTGGAT GACTCTGAGG AGGATGACGA TGAAGATAGC
GGACATAGCT CCAGAAGGAG GGGAAGCATT TCTAGTGGCG TTTCTTACGA AGAGTTTCAA
GTCCTGGTGA GACGAGTGGA CCGGATGGAG CATTCCATCG GCAGCATAGT GTCCAAGATT
GACGCCGTGA TCGTGAAGCT AGAGATTATG GAGCGAGCCA AACTGAAGAG GAGGGAGGTG
CTGGGAAGGC TGTTGGATGG GGTGGCCGAG GATGAAAGGC TGGGTCGTGA CAGTGAAATC
CATAGGGAAC AGATGGAACG GCTAGTACGT GAAGAGTTGG AACGCTGGGA ATCCGATGAT
GCAGCTTCCC AGATCAGTCA TGGTTTAGGC ACGCCAGTGG GACTAAATGG TCAACCTCGC
CCCAGAAGCT CCCGCCCATC TTCCTCCCAA TCTACAGAAG GCATGGAAGG TGCAGGTGGA
AATGGGAGTT CTAATGTCCA CGTATGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_011530332.1
CDS96..2162
Misc Feature(1)99..1316(+)
Misc Feature(2)1449..1589(+)
Misc Feature(3)1452..1577(+)
Translation

Target ORF information:

RefSeq Version XM_011532030
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens polycystic kidney disease 2 (autosomal dominant) (PKD2), transcript variant X3, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_011532030

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
ATGTTCACAG AAGGCTCCTT ATTGGATGGG CTGTACTGGA AGATGCAGCC CAGCAACCAG 
ACTGAAGCTG ACAACCGAAG TTTCATCTTC TATGAGAACC TGCTGTTAGG GGTTCCACGA
ATACGGCAAC TCCGAGTCAG AAATGGATCC TGCTCTATCC CCCAGGACTT GAGAGATGAA
ATTAAAGAGT GCTATGATGT CTACTCTGTC AGTAGTGAAG ATAGGGCTCC CTTTGGGCCC
CGAAATGGAA CCGCTTGGAT CTACACAAGT GAAAAAGACT TGAATGGTAG TAGCCACTGG
GGAATCATTG CAACTTATAG TGGAGCTGGC TATTATCTGG ATTTGTCAAG AACAAGAGAG
GAAACAGCTG CACAAGTTGC TAGCCTCAAG AAAAATGTCT GGCTGGACCG AGGAACCAGG
GCAACTTTTA TTGACTTCTC AGTGTACAAC GCCAACATTA ACCTGTTCTG TGTGGTCAGG
TTATTGGTTG AATTCCCAGC AACAGGTGGT GTGATTCCAT CTTGGCAATT TCAGCCTTTA
AAGCTGATCC GATATGTCAC AACTTTTGAT TTCTTCCTGG CAGCCTGTGA GATTATCTTT
TGTTTCTTTA TCTTTTACTA TGTGGTGGAA GAGATATTGG AAATTCGCAT TCACAAACTA
CACTATTTCA GGAGTTTCTG GAATTGTCTG GATGTTGTGA TCGTTGTGCT GTCAGTGGTA
GCTATAGGAA TTAACATATA CAGAACATCA AATGTGGAGG TGCTACTACA GTTTCTGGAA
GATCAAAATA CTTTCCCCAA CTTTGAGCAT CTGGCATATT GGCAGATACA GTTCAACAAT
ATAGCTGCTG TCACAGTATT TTTTGTCTGG ATTAAGCTCT TCAAATTCAT CAATTTTAAC
AGGACCATGA GCCAGCTCTC GACAACCATG TCTCGATGTG CCAAAGACCT GTTTGGCTTT
GCTATTATGT TCTTCATTAT TTTCCTAGCG TATGCTCAGT TGGCATACCT TGTCTTTGGC
ACTCAGGTCG ATGACTTCAG TACTTTCCAA GAGTGTATCT TCACTCAATT CCGTATCATT
TTGGGCGATA TCAACTTTGC AGAGATTGAG GAAGCTAATC GAGTTTTGGG ACCAATTTAT
TTCACTACAT TTGTGTTCTT TATGTTCTTC ATTCTTTTGA ATATGTTTTT GGCTATCATC
AATGATACTT ACTCTGAAGT GAAATCTGAC TTGGCACAGC AGAAAGCTGA AATGGAACTC
TCAGATCTTA TCAGAAAGGG CTACCATAAA GCTTTGGTCA AACTAAAACT GAAAAAAAAT
ACCGTGGATG ACATTTCAGA GAGTCTGCGG CAAGGAGGAG GCAAGTTAAA CTTTGACGAA
CTTCGACAAG ATCTCAAAGG GAAGGGCCAT ACTGATGCAG AGATTGAGGC AATATTCACA
AAGTACGACC AAGATGGAGA CCAAGAACTG ACCGAACATG AACATCAGCA GATGAGAGAC
GACTTGGAGA AAGAGAGGGA GGACCTGGAT TTGGATCACA GTTCTTTACC ACGTCCCATG
AGCAGCCGAA GTTTCCCTCG AAGCCTGGAT GACTCTGAGG AGGATGACGA TGAAGATAGC
GGACATAGCT CCAGAAGGAG GGGAAGCATT TCTAGTGGCG TTTCTTACGA AGAGTTTCAA
GTCCTGGTGA GACGAGTGGA CCGGATGGAG CATTCCATCG GCAGCATAGT GTCCAAGATT
GACGCCGTGA TCGTGAAGCT AGAGATTATG GAGCGAGCCA AACTGAAGAG GAGGGAGGTG
CTGGGAAGGC TGTTGGATGG GGTGGCCGAG GATGAAAGGC TGGGTCGTGA CAGTGAAATC
CATAGGGAAC AGATGGAACG GCTAGTACGT GAAGAGTTGG AACGCTGGGA ATCCGATGAT
GCAGCTTCCC AGATCAGTCA TGGTTTAGGC ACGCCAGTGG GACTAAATGG TCAACCTCGC
CCCAGAAGCT CCCGCCCATC TTCCTCCCAA TCTACAGAAG GCATGGAAGG TGCAGGTGGA
AATGGGAGTT CTAATGTCCA CGTATGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

book

Oligomerization of the polycystin-2 C-terminal tail and effects on its Ca2+-binding properties
J. Biol. Chem. 290 (16), 10544-10554 (2015)
Yang Y, Keeler C, Kuo IY, Lolis EJ, Ehrlich BE and Hodsdon ME.


book

Expression of polycystins and fibrocystin on primary cilia of lung cells
Biochem. Cell Biol. 92 (6), 547-554 (2014)
Hu Q, Wu Y, Tang J, Zheng W, Wang Q, Nahirney D, Duszyk M, Wang S, Tu JC and Chen XZ.


book

Altered trafficking and stability of polycystins underlie polycystic kidney disease
J. Clin. Invest. 124 (12), 5129-5144 (2014)
Cai,Y., Fedeles,S.V., Dong,K., Anyatonwu,G., Onoe,T., Mitobe,M., Gao,J.D., Okuhara,D., Tian,X., Gallagher,A.R., Tang,Z., Xie,X., Lalioti,M.D., Lee,A.H., Ehrlich,B.E. and Somlo,S.


book

A proteome-scale map of the human interactome network
Cell 159 (5), 1212-1226 (2014)
Rolland T, Tasan M, Charloteaux B, Pevzner SJ, Zhong Q, Sahni N, Yi S, Lemmens I, Fontanillo C, Mosca R, Kamburov A, Ghiassian SD, Yang X, Ghamsari L, Balcha D, Begg BE, Braun P, Brehme M, Broly MP, Carvunis AR, Convery-Zupan D, Corominas R, Coulombe-Huntington J, Dann E, Dreze M, Dricot A, Fan C, Franzosa E, Gebreab F, Gutierrez BJ, Hardy MF, Jin M, Kang S, Kiros R, Lin GN, Luck K, MacWilliams A, Menche J, Murray RR, Palagi A, Poulin MM, Rambout X, Rasla J, Reichert P, Romero V, Ruyssinck E, Sahalie JM, Scholz A, Shah AA, Sharma A, Shen Y, Spirohn K, Tam S, Tejeda AO, Trigg SA, Twizere JC, Vega K, Walsh J, Cusick ME, Xia Y, Barabasi AL, Iakoucheva LM, Aloy P, De Las Rivas J, Tavernier J, Calderwood MA, Hill DE, Hao T, Roth FP and Vidal M.


book

PKD1 interacts with PKD2 through a probable coiled-coil domain
Nat. Genet. 16 (2), 179-183 (1997)
Qian F, Germino FJ, Cai Y, Zhang X, Somlo S and Germino GG.


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A gene similar to PKD1 maps to chromosome 4q22: a candidate gene for PKD2
Genomics 38 (1), 1-4 (1996)
Schneider MC, Rodriguez AM, Nomura H, Zhou J, Morton CC, Reeders ST and Weremowicz S.


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PKD2, a gene for polycystic kidney disease that encodes an integral membrane protein
Science 272 (5266), 1339-1342 (1996)
Mochizuki T, Wu G, Hayashi T, Xenophontos SL, Veldhuisen B, Saris JJ, Reynolds DM, Cai Y, Gabow PA, Pierides A, Kimberling WJ, Breuning MH, Deltas CC, Peters DJ and Somlo S.


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Chromosome 4 localization of a second gene for autosomal dominant polycystic kidney disease
Nat. Genet. 5 (4), 359-362 (1993)
Peters DJ, Spruit L, Saris JJ, Ravine D, Sandkuijl LA, Fossdal R, Boersma J, van Eijk R, Norby S, Constantinou-Deltas CD et al.


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Congenital Hepatic Fibrosis Overview
(in) Pagon RA, Adam MP, Ardinger HH, Wallace SE, Amemiya A, Bean LJH, Bird TD, Dolan CR, Fong CT, Smith RJH and Stephens K (Eds.); GENEREVIEWS(R); (1993)
Gunay-Aygun,M., Gahl,W.A. and Heller,T.


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Polycystic Kidney Disease, Autosomal Dominant
(in) Pagon RA, Adam MP, Ardinger HH, Wallace SE, Amemiya A, Bean LJH, Bird TD, Fong CT, Smith RJH and Stephens K (Eds.); GENEREVIEWS(R); (1993)
Harris,P.C. and Torres,V.E.


 
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