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PLN cDNA ORF clone, Homo sapiens (human)

Gene Symbol PLN
Entrez Gene ID 5350
Full Name phospholamban
Synonyms CMD1P, CMH18, PLB
General protein information
Preferred Names
cardiac phospholamban
Names
cardiac phospholamban
Gene Type protein-coding
Organism Homo sapiens (human)
Genome

6

6q22.1

Summary The protein encoded by this gene is found as a pentamer and is a major substrate for the cAMP-dependent protein kinase in cardiac muscle. The encoded protein is an inhibitor of cardiac muscle sarcoplasmic reticulum Ca(2+)-ATPase in the unphosphorylated state, but inhibition is relieved upon phosphorylation of the protein. The subsequent activation of the Ca(2+) pump leads to enhanced muscle relaxation rates, thereby contributing to the inotropic response elicited in heart by beta-agonists. The encoded protein is a key regulator of cardiac diastolic function. Mutations in this gene are a cause of inherited human dilated cardiomyopathy with refractory congestive heart failure. [provided by RefSeq, Jul 2008]. lac of sum
Disorder MIM:

172405

Disorder Html: Cardiomyopathy, dilated, 1P, 609909 (3)

mRNA and Protein(s)

mRNA Protein Name
NM_002667 NP_002658 cardiac phospholamban



Homo sapiens (human) PLN NP_002658.1
Pan troglodytes (chimpanzee) PLN XP_003311500.1


GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following PLN gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the PLN cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

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***CloneID RefSeq Accession Definition **Vector *Turnaround time Price (USD) Select
OHu17925
NM_002667 Homo sapiens phospholamban (PLN), mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
$49.50-$69.30
$99.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.


CloneID OHu17925
Clone ID Related Accession (Same CDS sequence) NM_002667
Accession Version NM_002667.3 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 159bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product cardiac phospholamban
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BP259965.1, M63603.1 and BC005269.1. This sequence is a reference standard in the RefSeqGene project. On Jul 18, 2008 this sequence version replaced gi:29171725. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC005269.1, M63603.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
ATGGAGAAAG TCCAATACCT CACTCGCTCA GCTATAAGAA GAGCCTCAAC CATTGAAATG 
CCTCAACAAG CACGTCAAAA GCTACAGAAT CTATTTATCA ATTTCTGTCT CATCTTAATA
TGTCTCTTGC TGATCTGTAT CATCGTGATG CTTCTCTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_002658.1
CDS212..370
Misc Feature(1)98..100(+)
Misc Feature(2)212..367(+)
Misc Feature(3)257..277(+)
Misc Feature(4)257..259(+)
Misc Feature(5)257..259(+)
Misc Feature(6)260..262(+)
Misc Feature(7)260..262(+)
Misc Feature(8)305..367(+)
Exon (1)1..114
Gene:PLN
Gene Synonym:
Exon (2)115..1714
Gene:PLN
Gene Synonym:
Translation

Target ORF information:

RefSeq Version NM_002667
Organism Homo sapiens (human)
Definition Homo sapiens phospholamban (PLN), mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_002667

ORF Insert Sequence:

1
61
121
ATGGAGAAAG TCCAATACCT CACTCGCTCA GCTATAAGAA GAGCCTCAAC CATTGAAATG 
CCTCAACAAG CACGTCAAAA GCTACAGAAT CTATTTATCA ATTTCTGTCT CATCTTAATA
TGTCTCTTGC TGATCTGTAT CATCGTGATG CTTCTCTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

book

Arrhythmogenic right ventricular dysplasia/cardiomyopathy according to revised 2010 task force criteria with inclusion of non-desmosomal phospholamban mutation carriers
Am. J. Cardiol. 112 (8), 1197-1206 (2013)
Groeneweg JA, van der Zwaag PA, Olde Nordkamp LR, Bikker H, Jongbloed JD, Jongbloed R, Wiesfeld AC, Cox MG, van der Heijden JF, Atsma DE, de Boer K, Doevendans PA, Vink A, van Veen TA, Dooijes D, van den Berg MP, Wilde AA, van Tintelen JP and Hauer RN.


book

Genetic analysis in 418 index patients with idiopathic dilated cardiomyopathy: overview of 10 years' experience
Eur. J. Heart Fail. 15 (6), 628-636 (2013)
van Spaendonck-Zwarts KY, van Rijsingen IA, van den Berg MP, Lekanne Deprez RH, Post JG, van Mil AM, Asselbergs FW, Christiaans I, van Langen IM, Wilde AA, de Boer RA, Jongbloed JD, Pinto YM and van Tintelen JP.


book

Structure-function relation of phospholamban: modulation of channel activity as a potential regulator of SERCA activity
PLoS ONE 8 (1), E52744 (2013)
Smeazzetto S, Saponaro A, Young HS, Moncelli MR and Thiel G.


book

Impact of ancestry and common genetic variants on QT interval in African Americans
Circ Cardiovasc Genet 5 (6), 647-655 (2012)
Smith JG, Avery CL, Evans DS, Nalls MA, Meng YA, Smith EN, Palmer C, Tanaka T, Mehra R, Butler AM, Young T, Buxbaum SG, Kerr KF, Berenson GS, Schnabel RB, Li G, Ellinor PT, Magnani JW, Chen W, Bis JC, Curb JD, Hsueh WC, Rotter JI, Liu Y, Newman AB, Limacher MC, North KE, Reiner AP, Quibrera PM, Schork NJ, Singleton AB, Psaty BM, Soliman EZ, Solomon AJ, Srinivasan SR, Alonso A, Wallace R, Redline S, Zhang ZM, Post WS, Zonderman AB, Taylor HA, Murray SS, Ferrucci L, Arking DE, Evans MK, Fox ER, Sotoodehnia N, Heckbert SR, Whitsel EA and Newton-Cheh C.


book

Unchanged protein levels of SERCA II and phospholamban but reduced Ca2+ uptake and Ca(2+)-ATPase activity of cardiac sarcoplasmic reticulum from dilated cardiomyopathy patients compared with patients with nonfailing hearts
Circulation 92 (11), 3220-3228 (1995)
Schwinger RH, Bohm M, Schmidt U, Karczewski P, Bavendiek U, Flesch M, Krause EG and Erdmann E.


book

Computational searching and mutagenesis suggest a structure for the pentameric transmembrane domain of phospholamban
Nat. Struct. Biol. 2 (2), 154-162 (1995)
Adams PD, Arkin IT, Engelman DM and Brunger AT.


book

Hypertrophic Cardiomyopathy Overview
(in) Pagon RA, Adam MP, Ardinger HH, Bird TD, Dolan CR, Fong CT, Smith RJH and Stephens K (Eds.); GENEREVIEWS(R); (1993)
Cirino,A.L. and Ho,C.


book

Dilated Cardiomyopathy Overview
(in) Pagon RA, Adam MP, Ardinger HH, Bird TD, Dolan CR, Fong CT, Smith RJH and Stephens K (Eds.); GENEREVIEWS(R); (1993)
Hershberger,R.E. and Morales,A.


book

Structure of the rabbit phospholamban gene, cloning of the human cDNA, and assignment of the gene to human chromosome 6
J. Biol. Chem. 266 (18), 11669-11675 (1991)
Fujii J, Zarain-Herzberg A, Willard HF, Tada M and MacLennan DH.


book

Sequence analysis of phospholamban. Identification of phosphorylation sites and two major structural domains
J. Biol. Chem. 261 (28), 13333-13341 (1986)
Simmerman,H.K., Collins,J.H., Theibert,J.L., Wegener,A.D. and Jones,L.R.


 
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