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POU3F4 cDNA ORF clone, Homo sapiens(Human)

Gene Symbol POU3F4
Entrez Gene ID 5456
Full Name POU class 3 homeobox 4
Synonyms BRAIN-4,BRN-4,BRN4,DFN3,DFNX2,OCT-9,OTF-9,OTF9
General protein information
Preferred Names

POU class 3 homeobox 4

Names

POU domain, class 3, transcription factor 4
brain-specific homeobox/POU domain protein 4
octamer-binding transcription factor 9

Gene Type protein-coding
Organism Homo sapiens(Human)
Genome

X

Xq21.1

Summary This gene encodes a member of the POU-III class of neural transcription factors. This family member plays a role in inner ear development. The protein is thought to be involved in the mediation of epigenetic signals which induce striatal neuron-precursor differentiation. Mutations in this gene are associated with X chromosome-linked nonsyndromic mixed deafness. [provided by RefSeq, Dec 2012].
Disorder MIM:

300039

Disorder Html: Deafness, X-linked 2, 304400 (3)

mRNA and Protein(s)

mRNA Protein Name
NM_000307.4 NP_000298.3 POU domain, class 3, transcription factor 4



Pan troglodytes (chimpanzee) POU3F4 XP_003317585.1
Macaca mulatta (rhesus monkey) POU3F4 XP_001104059.1
Canis lupus familiaris (dog) POU3F4 XP_549108.1
Mus musculus (house mouse) Pou3f4 NP_032927.1
Rattus norvegicus (Norway rat) Pou3f4 NP_058948.1
Homo sapiens (human) POU3F4 NP_000298.3
Bos taurus (cattle) POU3F4 XP_002699986.1
Xenopus tropicalis (tropical clawed frog) pou3f4 NP_001090728.1


Related articles in PubMed

Cytoplasmic mislocalization of POU3F4 due to novel mutations leads to deafness in humans and mice.
Parzefall T, Shivatzki S, Lenz DR, Rathkolb B, Ushakov K, Karfunkel D, Shapira Y, Wolf M, Mohr M, Wolf E, Sabrautzki S, de Angelis MH, Frydman M, Brownstein Z, Avraham KB
Human mutation34(8)1102-10(2013 Aug)

Destabilization and mislocalization of POU3F4 by C-terminal frameshift truncation and extension mutation.
Choi BY, Kim DH, Chung T, Chang M, Kim EH, Kim AR, Seok J, Chang SO, Bok J, Kim D, Oh SH, Park WY
Human mutation34(2)309-16(2013 Feb)

Nonsyndromic X-linked hearing loss.
Song MH, Lee KY, Choi JY, Bok J, Kim UK
Frontiers in bioscience (Elite edition)4924-33(2012 Jan)

Common molecular etiologies are rare in nonsyndromic Tibetan Chinese patients with hearing impairment.
Yuan Y, Zhang X, Huang S, Zuo L, Zhang G, Song Y, Wang G, Wang H, Huang D, Han D, Dai P
PloS one7(2)e30720(2012)

Transcribed sequences encoded in the region involved in contiguous deletion syndrome that comprises X-linked stapes fixation and deafness.
Kandpal G, Jacob AN, Kandpal RP
Somatic cell and molecular genetics22(6)511-7(1996 Nov)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

POU3F4 mutations can be predicted by incomplete partition type III anomaly by radiological examination of the inner ear. All six of the patients showed mixed hearing loss, but none showed fluctuations in hearing, which may be related to the lack of vestibular aqueduct enlargement at the operculum.
Title: Frequency and specific characteristics of the incomplete partition type III anomaly in children.

A nonsense mutation is identified in a family displaying the pedigree consistent with X-linked recessive pattern in POU3F4 gene.
Title: A

POU3F4 mutation in profoundly deaf patients may have poorer prognosis after cochlear implantation, than other types.
Title: Clinical observations and molecular variables of patients with hearing loss and incomplete partition type III.

Sequencing of the entire POU3F4 gene is recommended in patients with characteristic temporal bone malformations. Results of POU3F4 mutation testing are important not only for a proper genetic counseling, but also for adequate preparation and conduction of a surgical procedure.
Title: Novel and De Novo Mutations Extend Association of POU3F4 with Distinct Clinical and Radiological Phenotype of Hearing Loss.

findings may greatly contribute to the elucidation of the roles of the Oct and Myc proteins in osteoblast direct reprogramming. The results may also lead to establishment of novel regenerative therapy for various bone resorption diseases
Title: Transduction of Oct6 or Oct9 gene concomitant with Myc family gene induced osteoblast-like phenotypic conversion in normal human fibroblasts.

The following POU3F4 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the POU3F4 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

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***CloneID RefSeq Accession Definition **Vector *Turnaround time Price (USD) Select
OHu20520 NM_000307.4
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Homo sapiens POU class 3 homeobox 4 (POU3F4), mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
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$299.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.


CloneID OHu20520
Clone ID Related Accession (Same CDS sequence) NM_000307.4
Accession Version NM_000307.4 Latest version! Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1086bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2014-05-04
Organism Homo sapiens(Human)
Product POU domain, class 3, transcription factor 4
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AK314967.1, Z82170.3 and X82324.1. This sequence is a reference standard in the RefSeqGene project. On Dec 26, 2012 this sequence version replaced NM_000307.3. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
ATGGCCACAG CTGCCTCGAA TCCCTACAGC ATTCTCAGTT CCACCTCCCT AGTCCATGCG 
GACTCTGCGG GCATGCAGCA GGGGAGTCCT TTCCGCAACC CTCAGAAACT TCTCCAAAGT
GATTACTTGC AGGGAGTTCC CAGCAATGGG CATCCCCTCG GGCATCACTG GGTGACCAGT
CTGAGCGACG GGGGCCCATG GTCCTCCACA CTGGCCACCA GCCCCCTGGA CCAGCAGGAC
GTGAAGCCCG GGCGCGAAGA CCTGCAACTG GGTGCGATCA TCCATCACCG CTCGCCACAC
GTAGCCCACC ACTCACCGCA CACTAACCAC CCCAACGCCT GGGGGGCCAG CCCGGCACCG
AACCCGTCTA TCACGTCAAG CGGCCAACCC CTCAACGTGT ACTCGCAGCC TGGCTTCACC
GTGAGCGGCA TGCTGGAACA CGGGGGACTC ACCCCACCTC CAGCTGCCGC CTCTGCACAG
AGCCTGCACC CGGTGCTCCG AGAGCCCCCG GATCACGGCG AACTGGGCTC GCACCATTGC
CAGGATCACT CCGACGAGGA GACGCCAACC TCTGATGAGT TGGAACAGTT CGCCAAACAA
TTCAAACAAA GAAGAATCAA GTTGGGCTTC ACGCAGGCCG ACGTGGGGTT GGCGCTGGGC
ACACTGTATG GTAACGTGTT CTCGCAGACC ACCATCTGCA GGTTCGAGGC CTTGCAGCTG
AGCTTCAAAA ATATGTGCAA GCTGAAGCCC CTGCTGAACA AGTGGCTGGA GGAGGCGGAT
TCGTCCACAG GGAGCCCGAC CAGCATTGAC AAGATCGCTG CACAGGGCCG CAAGCGCAAG
AAGCGGACCT CCATCGAGGT GAGTGTCAAG GGCGTACTGG AGACGCATTT CCTCAAGTGT
CCCAAGCCTG CCGCGCAGGA GATCTCCTCG CTGGCAGACA GCCTCCAGTT GGAGAAGGAA
GTGGTGCGTG TCTGGTTCTG TAATCGAAGA CAAAAAGAGA AAAGAATGAC TCCGCCAGGG
GATCAGCAGC CGCATGAGGT TTATTCGCAC ACCGTGAAAA CAGACACATC TTGCCATGAT
CTCTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_000298.3
CDS65..1150
Misc Feature(1)14..16
Misc Feature(2)620..844
Misc Feature(3)order(899..913,917..919,968..970,986..988,1025..1027,1031..1036,1043..1048,1052..1060,1064..1069)
Misc Feature(4)905..1066
Misc Feature(5)order(905..907,914..916,1034..1036,1043..1048,1055..1057)
Exon (1)1..1507
Translation

Target ORF information:

RefSeq Version NM_000307.4
Organism Homo sapiens(Human)
Definition Homo sapiens POU class 3 homeobox 4 (POU3F4), mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_000307.4

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
ATGGCCACAG CTGCCTCGAA TCCCTACAGC ATTCTCAGTT CCACCTCCCT AGTCCATGCG 
GACTCTGCGG GCATGCAGCA GGGGAGTCCT TTCCGCAACC CTCAGAAACT TCTCCAAAGT
GATTACTTGC AGGGAGTTCC CAGCAATGGG CATCCCCTCG GGCATCACTG GGTGACCAGT
CTGAGCGACG GGGGCCCATG GTCCTCCACA CTGGCCACCA GCCCCCTGGA CCAGCAGGAC
GTGAAGCCCG GGCGCGAAGA CCTGCAACTG GGTGCGATCA TCCATCACCG CTCGCCACAC
GTAGCCCACC ACTCACCGCA CACTAACCAC CCCAACGCCT GGGGGGCCAG CCCGGCACCG
AACCCGTCTA TCACGTCAAG CGGCCAACCC CTCAACGTGT ACTCGCAGCC TGGCTTCACC
GTGAGCGGCA TGCTGGAACA CGGGGGACTC ACCCCACCTC CAGCTGCCGC CTCTGCACAG
AGCCTGCACC CGGTGCTCCG AGAGCCCCCG GATCACGGCG AACTGGGCTC GCACCATTGC
CAGGATCACT CCGACGAGGA GACGCCAACC TCTGATGAGT TGGAACAGTT CGCCAAACAA
TTCAAACAAA GAAGAATCAA GTTGGGCTTC ACGCAGGCCG ACGTGGGGTT GGCGCTGGGC
ACACTGTATG GTAACGTGTT CTCGCAGACC ACCATCTGCA GGTTCGAGGC CTTGCAGCTG
AGCTTCAAAA ATATGTGCAA GCTGAAGCCC CTGCTGAACA AGTGGCTGGA GGAGGCGGAT
TCGTCCACAG GGAGCCCGAC CAGCATTGAC AAGATCGCTG CACAGGGCCG CAAGCGCAAG
AAGCGGACCT CCATCGAGGT GAGTGTCAAG GGCGTACTGG AGACGCATTT CCTCAAGTGT
CCCAAGCCTG CCGCGCAGGA GATCTCCTCG CTGGCAGACA GCCTCCAGTT GGAGAAGGAA
GTGGTGCGTG TCTGGTTCTG TAATCGAAGA CAAAAAGAGA AAAGAATGAC TCCGCCAGGG
GATCAGCAGC CGCATGAGGT TTATTCGCAC ACCGTGAAAA CAGACACATC TTGCCATGAT
CTCTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

book

Cytoplasmic mislocalization of POU3F4 due to novel mutations leads to deafness in humans and mice.
Human mutation34(8)1102-10(2013 Aug)
Parzefall T,Shivatzki S,Lenz DR,Rathkolb B,Ushakov K,Karfunkel D,Shapira Y,Wolf M,Mohr M,Wolf E,Sabrautzki S,de Angelis MH,Frydman M,Brownstein Z,Avraham KB


book

Destabilization and mislocalization of POU3F4 by C-terminal frameshift truncation and extension mutation.
Human mutation34(2)309-16(2013 Feb)
Choi BY,Kim DH,Chung T,Chang M,Kim EH,Kim AR,Seok J,Chang SO,Bok J,Kim D,Oh SH,Park WY


book

Nonsyndromic X-linked hearing loss.
Frontiers in bioscience (Elite edition)4924-33(2012 Jan)
Song MH,Lee KY,Choi JY,Bok J,Kim UK


book

Common molecular etiologies are rare in nonsyndromic Tibetan Chinese patients with hearing impairment.
PloS one7(2)e30720(2012)
Yuan Y,Zhang X,Huang S,Zuo L,Zhang G,Song Y,Wang G,Wang H,Huang D,Han D,Dai P


book

Transcribed sequences encoded in the region involved in contiguous deletion syndrome that comprises X-linked stapes fixation and deafness.
Somatic cell and molecular genetics22(6)511-7(1996 Nov)
Kandpal G,Jacob AN,Kandpal RP


 
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