Gene Symbol | WDR74 |
Entrez Gene ID | 54663 |
Full Name | WD repeat domain 74 |
General protein information |
|
Gene Type | protein-coding |
Organism | Homo sapiens(human) |
Genome |
ORF » Species Summary » Homo sapiens » WDR74 cDNA ORF clone
Gene Symbol | WDR74 |
Entrez Gene ID | 54663 |
Full Name | WD repeat domain 74 |
General protein information |
|
Gene Type | protein-coding |
Organism | Homo sapiens(human) |
Genome |
mRNA | Protein | Name |
---|---|---|
NM_018093.3 | NP_060563.2 | WD repeat-containing protein 74 isoform 1 |
NM_001369449.1 | NP_001356378.1 | WD repeat-containing protein 74 isoform 4 |
XM_005274055.2 | XP_005274112.1 | WD repeat-containing protein 74 isoform X1 |
XM_024448585.1 | XP_024304353.1 | WD repeat-containing protein 74 isoform X1 |
NM_001369450.1 | NP_001356379.1 | WD repeat-containing protein 74 isoform 1 |
NM_001307977.1 | NP_001294906.1 | WD repeat-containing protein 74 isoform 2 |
XM_024448586.1 | XP_024304354.1 | WD repeat-containing protein 74 isoform X2 |
XM_005274055.3 | XP_005274112.1 | WD repeat-containing protein 74 isoform X3 |
NM_001369447.1 | NP_001356376.1 | WD repeat-containing protein 74 isoform 3 |
XM_005274055.1 | XP_005274112.1 | WD repeat-containing protein 74 isoform X1 |
NM_001369451.1 | NP_001356380.1 | WD repeat-containing protein 74 isoform 1 |
NM_001369448.1 | NP_001356377.1 | WD repeat-containing protein 74 isoform 4 |
NM_001369453.1 | NP_001356382.1 | WD repeat-containing protein 74 isoform 1 |
Canis lupus familiaris (dog) | WDR74 | XP_540899.2 |
WDR74 | XP_001116087.1 | |
Rattus norvegicus (Norway rat) | Wdr74 | NP_001103039.1 |
Bos taurus (cattle) | WDR74 | NP_001014854.1 |
Mus musculus (house mouse) | Wdr74 | NP_598900.1 |
Gallus gallus (chicken) | WDR74 | XP_003643844.2 |
Caenorhabditis elegans (roundworm) | CELE_T06E6.1 | NP_506824.1 |
Homo sapiens (human) | WDR74 | NP_060563.2 |
Danio rerio (zebrafish) | wdr74 | NP_001070031.1 |
Xenopus tropicalis (tropical clawed frog) | wdr74 | NP_001005144.1 |
WDR74 participates in an early cleavage of the pre-rRNA processing pathway in cooperation with the nucleolar AAA-ATPase NVL2.
Hiraishi N, Ishida YI, Sudo H, Nagahama M
Biochemical and biophysical research communications495(1)116-123(2018 Jan)
Structural Analysis Reveals Features of Ribosome Assembly Factor Nsa1/WDR74 Important for Localization and Interaction with Rix7/NVL2.
Lo YH, Romes EM, Pillon MC, Sobhany M, Stanley RE
Structure (London, England : 1993)25(5)762-772.e4(2017 May)
AAA-ATPase NVL2 acts on MTR4-exosome complex to dissociate the nucleolar protein WDR74.
Hiraishi N, Ishida Y, Nagahama M
Biochemical and biophysical research communications467(3)534-40(2015 Nov)
NO66, a highly conserved dual location protein in the nucleolus and in a special type of synchronously replicating chromatin.
Eilbracht J, Reichenzeller M, Hergt M, Schn?lzer M, Heid H, St?hr M, Franke WW, Schmidt-Zachmann MS
Molecular biology of the cell15(4)1816-32(2004 Apr)
GeneRIFs: Gene References Into Functions What's a GeneRIF?
knockdown of WDR74 leads to significant defects in the pre-rRNA cleavage within the internal transcribed spacer 1, occurring in an early stage of the processing pathway. When the dissociation of WDR74 from the MTR4-containing exonuclease complex was impaired upon expression of mutant NVL2, the same processing defect, with partial migration of WDR74 from the nucleolus towards the nucleoplasm, was observed.
Title: WDR74 participates in an early cleavage of the pre-rRNA processing pathway in cooperation with the nucleolar AAA-ATPase NVL2.
results suggest that WDR74 is a novel regulatory protein of the MTR4-exsosome complex whose interaction is regulated by NVL2 and is involved in ribosome biogenesis
Title: AAA-ATPase NVL2 acts on MTR4-exosome complex to dissociate the nucleolar protein WDR74.
The following WDR74 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the WDR74 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.
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CloneID | OHu31428 | |
Clone ID Related Accession (Same CDS sequence) | NM_018093.3 , XM_005274055.2 , NM_001369450.1 , XM_005274055.3 , XM_005274055.1 , NM_001369451.1 , NM_001369453.1 | |
Accession Version | NM_018093.3 Latest version! | Documents for ORF clone product in default vector |
Sequence Information | ORF Nucleotide Sequence (Length: 1158bp) Protein sequence SNP |
|
Vector | pcDNA3.1-C-(k)DYK or customized vector | User Manual |
Clone information | Clone Map | MSDS |
Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
ORF Insert Method | CloneEZ™ Seamless cloning technology | |
Insert Structure | linear | |
Update Date | 2019-09-09 | |
Organism | Homo sapiens(human) | |
Product | WD repeat-containing protein 74 isoform 1 | |
Comment | Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AK001301.1, AY500828.1, BC006351.2 and BU740579.1. On Apr 19, 2015 this sequence version replaced NM_018093.2. Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). ##Evidence-Data-START## Transcript exon combination :: AK001301.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end. |
1 | ATGGCGGCTG CTGCTGCACG CTGGAACCAT GTGTGGGTCG GCACCGAGAC TGGGATCTTG |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
RefSeq | NP_060563.2 |
CDS | 169..1326 |
Translation |
Target ORF information:
Target ORF information:
|
NM_018093.3 |
1 | ATGGCGGCTG CTGCTGCACG CTGGAACCAT GTGTGGGTCG GCACCGAGAC TGGGATCTTG |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
CloneID | OHu31428 | |
Clone ID Related Accession (Same CDS sequence) | NM_018093.3 , XM_005274055.2 , NM_001369450.1 , XM_005274055.3 , XM_005274055.1 , NM_001369451.1 , NM_001369453.1 | |
Accession Version | NM_001369450.1 Latest version! | Documents for ORF clone product in default vector |
Sequence Information | ORF Nucleotide Sequence (Length: 1158bp) Protein sequence SNP |
|
Vector | pcDNA3.1-C-(k)DYK or customized vector | User Manual |
Clone information | Clone Map | MSDS |
Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
ORF Insert Method | CloneEZ™ Seamless cloning technology | |
Insert Structure | linear | |
Update Date | 2019-09-09 | |
Organism | Homo sapiens(human) | |
Product | WD repeat-containing protein 74 isoform 1 | |
Comment | Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AP001160.4. On May 20, 2019 this sequence version replaced NM_001369452.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## Transcript exon combination :: SRR5189658.131235.1, SRR7410570.472165.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## RefSeq Select criteria :: based on conservation, expression ##RefSeq-Attributes-END## |
1 | ATGGCGGCTG CTGCTGCACG CTGGAACCAT GTGTGGGTCG GCACCGAGAC TGGGATCTTG |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
RefSeq | NP_001356379.1 |
CDS | 21..1178 |
Translation |
Target ORF information:
Target ORF information:
|
NM_001369450.1 |
1 | ATGGCGGCTG CTGCTGCACG CTGGAACCAT GTGTGGGTCG GCACCGAGAC TGGGATCTTG |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
CloneID | OHu31428 | |
Clone ID Related Accession (Same CDS sequence) | NM_018093.3 , XM_005274055.2 , NM_001369450.1 , XM_005274055.3 , XM_005274055.1 , NM_001369451.1 , NM_001369453.1 | |
Accession Version | NM_001369451.1 Latest version! | Documents for ORF clone product in default vector |
Sequence Information | ORF Nucleotide Sequence (Length: 1158bp) Protein sequence SNP |
|
Vector | pcDNA3.1-C-(k)DYK or customized vector | User Manual |
Clone information | Clone Map | MSDS |
Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
ORF Insert Method | CloneEZ™ Seamless cloning technology | |
Insert Structure | linear | |
Update Date | 2019-09-09 | |
Organism | Homo sapiens(human) | |
Product | WD repeat-containing protein 74 isoform 1 | |
Comment | Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AP001160.4. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## Transcript exon combination :: AK292330.1, SRR1163655.481287.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## |
1 | ATGGCGGCTG CTGCTGCACG CTGGAACCAT GTGTGGGTCG GCACCGAGAC TGGGATCTTG |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
RefSeq | NP_001356380.1 |
CDS | 539..1696 |
Translation |
Target ORF information:
Target ORF information:
|
NM_001369451.1 |
1 | ATGGCGGCTG CTGCTGCACG CTGGAACCAT GTGTGGGTCG GCACCGAGAC TGGGATCTTG |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
CloneID | OHu31428 | |
Clone ID Related Accession (Same CDS sequence) | NM_018093.3 , XM_005274055.2 , NM_001369450.1 , XM_005274055.3 , XM_005274055.1 , NM_001369451.1 , NM_001369453.1 | |
Accession Version | NM_001369453.1 Latest version! | Documents for ORF clone product in default vector |
Sequence Information | ORF Nucleotide Sequence (Length: 1158bp) Protein sequence SNP |
|
Vector | pcDNA3.1-C-(k)DYK or customized vector | User Manual |
Clone information | Clone Map | MSDS |
Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
ORF Insert Method | CloneEZ™ Seamless cloning technology | |
Insert Structure | linear | |
Update Date | 2019-09-09 | |
Organism | Homo sapiens(human) | |
Product | WD repeat-containing protein 74 isoform 1 | |
Comment | Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AP001160.4. On Apr 9, 2019 this sequence version replaced XM_005274055.3. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## Transcript exon combination :: SRR1803616.332376.1, SRR1163658.430983.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## |
1 | ATGGCGGCTG CTGCTGCACG CTGGAACCAT GTGTGGGTCG GCACCGAGAC TGGGATCTTG |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
RefSeq | NP_001356382.1 |
CDS | 431..1588 |
Translation |
Target ORF information:
Target ORF information:
|
NM_001369453.1 |
1 | ATGGCGGCTG CTGCTGCACG CTGGAACCAT GTGTGGGTCG GCACCGAGAC TGGGATCTTG |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
CloneID | OHu31428 | |
Clone ID Related Accession (Same CDS sequence) | NM_018093.3 , XM_005274055.2 , NM_001369450.1 , XM_005274055.3 , XM_005274055.1 , NM_001369451.1 , NM_001369453.1 | |
Accession Version | XM_005274055.2 | Documents for ORF clone product in default vector |
Sequence Information | ORF Nucleotide Sequence (Length: 1158bp) Protein sequence SNP |
|
Vector | pcDNA3.1-C-(k)DYK or customized vector | User Manual |
Clone information | Clone Map | MSDS |
Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
ORF Insert Method | CloneEZ™ Seamless cloning technology | |
Insert Structure | linear | |
Update Date | 2016-06-05 | |
Organism | Homo sapiens(human) | |
Product | WD repeat-containing protein 74 isoform X1 | |
Comment | Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000011.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_005274055.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## |
1 | ATGGCGGCTG CTGCTGCACG CTGGAACCAT GTGTGGGTCG GCACCGAGAC TGGGATCTTG |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
RefSeq | XP_005274112.1 |
CDS | 2030..3187 |
Translation |
Target ORF information:
Target ORF information:
|
XM_005274055.2 |
1 | ATGGCGGCTG CTGCTGCACG CTGGAACCAT GTGTGGGTCG GCACCGAGAC TGGGATCTTG |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
CloneID | OHu31428 | |
Clone ID Related Accession (Same CDS sequence) | NM_018093.3 , XM_005274055.2 , NM_001369450.1 , XM_005274055.3 , XM_005274055.1 , NM_001369451.1 , NM_001369453.1 | |
Accession Version | XM_005274055.3 Latest version! | Documents for ORF clone product in default vector |
Sequence Information | ORF Nucleotide Sequence (Length: 1158bp) Protein sequence SNP |
|
Vector | pcDNA3.1-C-(k)DYK or customized vector | User Manual |
Clone information | Clone Map | MSDS |
Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
ORF Insert Method | CloneEZ™ Seamless cloning technology | |
Insert Structure | linear | |
Update Date | 2018-03-25 | |
Organism | Homo sapiens(human) | |
Product | WD repeat-containing protein 74 isoform X3 | |
Comment | Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000011.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_005274055.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Homo sapiens Annotation Release 109 Annotation Version :: 109 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## |
1 | ATGGCGGCTG CTGCTGCACG CTGGAACCAT GTGTGGGTCG GCACCGAGAC TGGGATCTTG |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
RefSeq | XP_005274112.1 |
CDS | 647..1804 |
Translation |
Target ORF information:
Target ORF information:
|
XM_005274055.3 |
1 | ATGGCGGCTG CTGCTGCACG CTGGAACCAT GTGTGGGTCG GCACCGAGAC TGGGATCTTG |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
CloneID | OHu31428 | |
Clone ID Related Accession (Same CDS sequence) | NM_018093.3 , XM_005274055.2 , NM_001369450.1 , XM_005274055.3 , XM_005274055.1 , NM_001369451.1 , NM_001369453.1 | |
Accession Version | XM_005274055.1 | Documents for ORF clone product in default vector |
Sequence Information | ORF Nucleotide Sequence (Length: 1158bp) Protein sequence SNP |
|
Vector | pcDNA3.1-C-(k)DYK or customized vector | User Manual |
Clone information | Clone Map | MSDS |
Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
ORF Insert Method | CloneEZ™ Seamless cloning technology | |
Insert Structure | linear | |
Update Date | 2015-03-11 | |
Organism | Homo sapiens(Human) | |
Product | WD repeat-containing protein 74 isoform X1 | |
Comment |
1 | ATGGCGGCTG CTGCTGCACG CTGGAACCAT GTGTGGGTCG GCACCGAGAC TGGGATCTTG |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
RefSeq | XP_005274112.1 |
Translation |
Target ORF information:
Target ORF information:
|
XM_005274055.1 |
1 | ATGGCGGCTG CTGCTGCACG CTGGAACCAT GTGTGGGTCG GCACCGAGAC TGGGATCTTG |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
CloneID | OHu68485 | |
Clone ID Related Accession (Same CDS sequence) | NM_001307977.1 | |
Accession Version | NM_001307977.1 Latest version! | Documents for ORF clone product in default vector |
Sequence Information | ORF Nucleotide Sequence (Length: 1101bp) Protein sequence SNP |
|
Vector | pcDNA3.1-C-(k)DYK or customized vector | User Manual |
Clone information | Clone Map | MSDS |
Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
ORF Insert Method | CloneEZ™ Seamless cloning technology | |
Insert Structure | linear | |
Update Date | 2019-09-09 | |
Organism | Homo sapiens(human) | |
Product | WD repeat-containing protein 74 isoform 2 | |
Comment | Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AK001301.1, BC006351.2 and BU740579.1. On Apr 19, 2015 this sequence version replaced XM_011545104.1. Transcript Variant: This variant (2) differs in the 5' UTR and lacks an in-frame exon in the 3' coding region compared to variant 1. It encodes isoform 2, which is shorter than isoform 1. ##Evidence-Data-START## Transcript exon combination :: BC006351.2 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end. |
1 | ATGGCGGCTG CTGCTGCACG CTGGAACCAT GTGTGGGTCG GCACCGAGAC TGGGATCTTG |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
RefSeq | NP_001294906.1 |
CDS | 90..1190 |
Translation |
Target ORF information:
Target ORF information:
|
NM_001307977.1 |
1 | ATGGCGGCTG CTGCTGCACG CTGGAACCAT GTGTGGGTCG GCACCGAGAC TGGGATCTTG |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
CloneID | OHu107999 | |
Clone ID Related Accession (Same CDS sequence) | NM_001369449.1 , NM_001369448.1 | |
Accession Version | NM_001369449.1 Latest version! | Documents for ORF clone product in default vector |
Sequence Information | ORF Nucleotide Sequence (Length: 753bp) Protein sequence SNP |
|
Vector | pcDNA3.1-C-(k)DYK or customized vector | User Manual |
Clone information | Clone Map | MSDS |
Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
ORF Insert Method | CloneEZ™ Seamless cloning technology | |
Insert Structure | linear | |
Update Date | 2019-09-09 | |
Organism | Homo sapiens(human) | |
Product | WD repeat-containing protein 74 isoform 4 | |
Comment | Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AP001160.4. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## Transcript exon combination :: SRR3476690.264193.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## |
1 | ATGCGCCAAG ACCCAGCACA CCCCCATGTG GTTGCCACAG GTGGGAAAGA GAATGCTTTG |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
RefSeq | NP_001356378.1 |
CDS | 1013..1765 |
Translation |
Target ORF information:
Target ORF information:
|
NM_001369449.1 |
1 | ATGCGCCAAG ACCCAGCACA CCCCCATGTG GTTGCCACAG GTGGGAAAGA GAATGCTTTG |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
CloneID | OHu107999 | |
Clone ID Related Accession (Same CDS sequence) | NM_001369449.1 , NM_001369448.1 | |
Accession Version | NM_001369448.1 Latest version! | Documents for ORF clone product in default vector |
Sequence Information | ORF Nucleotide Sequence (Length: 753bp) Protein sequence SNP |
|
Vector | pcDNA3.1-C-(k)DYK or customized vector | User Manual |
Clone information | Clone Map | MSDS |
Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
ORF Insert Method | CloneEZ™ Seamless cloning technology | |
Insert Structure | linear | |
Update Date | 2019-09-09 | |
Organism | Homo sapiens(human) | |
Product | WD repeat-containing protein 74 isoform 4 | |
Comment | Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AP001160.4. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## Transcript exon combination :: SRR7346977.416051.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## |
1 | ATGCGCCAAG ACCCAGCACA CCCCCATGTG GTTGCCACAG GTGGGAAAGA GAATGCTTTG |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
RefSeq | NP_001356377.1 |
CDS | 548..1300 |
Translation |
Target ORF information:
Target ORF information:
|
NM_001369448.1 |
1 | ATGCGCCAAG ACCCAGCACA CCCCCATGTG GTTGCCACAG GTGGGAAAGA GAATGCTTTG |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
CloneID | OHu108001 | |
Clone ID Related Accession (Same CDS sequence) | NM_001369447.1 | |
Accession Version | NM_001369447.1 Latest version! | Documents for ORF clone product in default vector |
Sequence Information | ORF Nucleotide Sequence (Length: 1200bp) Protein sequence SNP |
|
Vector | pcDNA3.1-C-(k)DYK or customized vector | User Manual |
Clone information | Clone Map | MSDS |
Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
ORF Insert Method | CloneEZ™ Seamless cloning technology | |
Insert Structure | linear | |
Update Date | 2019-09-09 | |
Organism | Homo sapiens(human) | |
Product | WD repeat-containing protein 74 isoform 3 | |
Comment | Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AP001160.4. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## Transcript exon combination :: SRR7410570.472164.1, SRR1163655.11003.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## |
1 | ATGGCGGCTG CTGCTGCACG CTGGAACCAT GTGTGGGTCG GCACCGAGAC TGGGATCTTG |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
RefSeq | NP_001356376.1 |
CDS | 21..1220 |
Translation |
Target ORF information:
Target ORF information:
|
NM_001369447.1 |
1 | ATGGCGGCTG CTGCTGCACG CTGGAACCAT GTGTGGGTCG GCACCGAGAC TGGGATCTTG |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
CloneID | OHu106681 | |
Clone ID Related Accession (Same CDS sequence) | XM_024448585.1 | |
Accession Version | XM_024448585.1 Latest version! | Documents for ORF clone product in default vector |
Sequence Information | ORF Nucleotide Sequence (Length: 1434bp) Protein sequence SNP |
|
Vector | pcDNA3.1-C-(k)DYK or customized vector | User Manual |
Clone information | Clone Map | MSDS |
Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
ORF Insert Method | CloneEZ™ Seamless cloning technology | |
Insert Structure | linear | |
Update Date | 2018-03-25 | |
Organism | Homo sapiens(human) | |
Product | WD repeat-containing protein 74 isoform X1 | |
Comment | Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000011.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Homo sapiens Annotation Release 109 Annotation Version :: 109 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## |
1 | ATGTATTCTT CCGATCTACA TAAACAAGCA CCTCCTGGTT TCATTTTCGT AAAGCAAAAC |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
RefSeq | XP_024304353.1 |
CDS | 457..1890 |
Translation |
Target ORF information:
Target ORF information:
|
XM_024448585.1 |
1 | ATGTATTCTT CCGATCTACA TAAACAAGCA CCTCCTGGTT TCATTTTCGT AAAGCAAAAC |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
CloneID | OHu106682 | |
Clone ID Related Accession (Same CDS sequence) | XM_024448586.1 | |
Accession Version | XM_024448586.1 Latest version! | Documents for ORF clone product in default vector |
Sequence Information | ORF Nucleotide Sequence (Length: 1275bp) Protein sequence SNP |
|
Vector | pcDNA3.1-C-(k)DYK or customized vector | User Manual |
Clone information | Clone Map | MSDS |
Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
ORF Insert Method | CloneEZ™ Seamless cloning technology | |
Insert Structure | linear | |
Update Date | 2018-03-25 | |
Organism | Homo sapiens(human) | |
Product | WD repeat-containing protein 74 isoform X2 | |
Comment | Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000011.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Homo sapiens Annotation Release 109 Annotation Version :: 109 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## |
1 | ATGGCGGCTG CTGCTGCACG CTGGAACCAT GTGTGGGTCG GCACCGAGAC TGGGATCTTG |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
RefSeq | XP_024304354.1 |
CDS | 48..1322 |
Translation |
Target ORF information:
Target ORF information:
|
XM_024448586.1 |
1 | ATGGCGGCTG CTGCTGCACG CTGGAACCAT GTGTGGGTCG GCACCGAGAC TGGGATCTTG |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
WDR74 participates in an early cleavage of the pre-rRNA processing pathway in cooperation with the nucleolar AAA-ATPase NVL2. |
Structural Analysis Reveals Features of Ribosome Assembly Factor Nsa1/WDR74 Important for Localization and Interaction with Rix7/NVL2. |
AAA-ATPase NVL2 acts on MTR4-exosome complex to dissociate the nucleolar protein WDR74. |
NO66, a highly conserved dual location protein in the nucleolus and in a special type of synchronously replicating chromatin. |