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MKS1 cDNA ORF clone, Homo sapiens(Human)

Gene Symbol MKS1
Entrez Gene ID 54903
Full Name Meckel syndrome, type 1
Synonyms BBS13,JBTS28,MES,MKS,POC12
General protein information
Preferred Names

Meckel syndrome, type 1

Names

Meckel syndrome type 1 protein
POC12 centriolar protein homolog

Gene Type protein-coding
Organism Homo sapiens(Human)
Genome

17

17q22

Summary The protein encoded by this gene localizes to the basal body and is required for formation of the primary cilium in ciliated epithelial cells. Mutations in this gene result in Meckel syndrome type 1 and in Bardet-Biedl syndrome type 13. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2009].
Disorder MIM:

609883

Disorder Html: Meckel syndrome, type 1, 249000 (3); Bardet-Biedl syndrome 13,

mRNA and Protein(s)

mRNA Protein Name
NM_001321268.1 NP_001308197.1 Meckel syndrome type 1 protein isoform 3
XM_006721965.2 XP_006722028.1 Meckel syndrome type 1 protein isoform X9
XM_011524957.2 XP_011523259.1 Meckel syndrome type 1 protein isoform X1
XM_011524959.2 XP_011523261.1 Meckel syndrome type 1 protein isoform X3
XM_011524960.2 XP_011523262.1 Meckel syndrome type 1 protein isoform X5
XM_017024804.1 XP_016880293.1 Meckel syndrome type 1 protein isoform X6
XM_005257485.3 XP_005257542.1 Meckel syndrome type 1 protein isoform X7
NM_001330397.1 NP_001317326.1 Meckel syndrome type 1 protein isoform 5
NM_001165927.1 NP_001159399.1 Meckel syndrome type 1 protein isoform 2
NM_017777.3 NP_060247.2 Meckel syndrome type 1 protein isoform 1
XM_011524958.2 XP_011523260.1 Meckel syndrome type 1 protein isoform X2
NM_001321269.1 NP_001308198.1 Meckel syndrome type 1 protein isoform 4
XM_017024805.1 XP_016880294.1 Meckel syndrome type 1 protein isoform X8



Bos taurus (cattle) LOC101905689 XP_005220007.1
Rattus norvegicus (Norway rat) Mks1 NP_001030089.2
Pan troglodytes (chimpanzee) MKS1 XP_001172592.1
Mus musculus (house mouse) Mks1 NP_001034773.2
Gallus gallus (chicken) MKS1 XP_415705.3
Danio rerio (zebrafish) mks1 NP_001070841.2
Xenopus tropicalis (tropical clawed frog) mks1 NP_001039150.1
Canis lupus familiaris (dog) MKS1 XP_003639341.1
Homo sapiens (human) MKS1 NP_060247.2


Related articles in PubMed

MKS1 regulates ciliary INPP5E levels in Joubert syndrome.
Slaats GG, Isabella CR, Kroes HY, Dempsey JC, Gremmels H, Monroe GR, Phelps IG, Duran KJ, Adkins J, Kumar SA, Knutzen DM, Knoers NV, Mendelsohn NJ, Neubauer D, Mastroyianni SD, Vogt J, Worgan L, Karp N, Bowdin S, Glass IA, Parisi MA, Otto EA, Johnson CA, Hildebrandt F, van Haaften G, Giles RH, Doherty D
Journal of medical genetics53(1)62-72(2016 Jan)

Mutations in B9D1 and MKS1 cause mild Joubert syndrome: expanding the genetic overlap with the lethal ciliopathy Meckel syndrome.
Romani M, Micalizzi A, Kraoua I, Dotti MT, Cavallin M, Sztriha L, Ruta R, Mancini F, Mazza T, Castellana S, Hanene B, Carluccio MA, Darra F, Máté A, Zimmermann A, Gouider-Khouja N, Valente EM
Orphanet journal of rare diseases972(2014 May)

Mutation analysis of 18 nephronophthisis associated ciliopathy disease genes using a DNA pooling and next generation sequencing strategy.
Otto EA, Ramaswami G, Janssen S, Chaki M, Allen SJ, Zhou W, Airik R, Hurd TW, Ghosh AK, Wolf MT, Hoppe B, Neuhaus TJ, Bockenhauer D, Milford DV, Soliman NA, Antignac C, Saunier S, Johnson CA, Hildebrandt F,
Journal of medical genetics48(2)105-16(2011 Feb)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Dnah11(avc)(4) did not disrupt SHF Hh signaling and caused Atrioventricular septal defects (AVSDs) only concurrently with heterotaxy, a left/right axis abnormality. In contrast, Mks1(avc)(6) disrupted SHF Hh signaling and caused AVSDs without heterotaxy.We speculate that cilia gene mutations contribute to both syndromic and non-syndromic AVSDs in humans
Title: Cilia gene mutations cause atrioventricular septal defects by multiple mechanisms.

MKS1 functions in the transition zone at the base of the cilium to regulate ciliary INPP5E content.
Title: MKS1 regulates ciliary INPP5E levels in Joubert syndrome.

describe four patients with mild Joubert phenotypes who carry pathogenic mutations in either MKS1 or B9D1, two genes previously implicated only in Meckel syndrome
Title: Mutations in B9D1 and MKS1 cause mild Joubert syndrome: expanding the genetic overlap with the lethal ciliopathy Meckel syndrome.

Observational study of gene-disease association. (HuGE Navigator)
Title: Mutation analysis of 18 nephronophthisis associated ciliopathy disease genes using a DNA pooling and next generation sequencing strategy.

Kidney tissue and cells from MKS1 and MKS3 patients showed defects in centrosome and cilia number, including multi-ciliated respiratory-like epithelia, and longer cilia.
Title: Ciliary and centrosomal defects associated with mutation and depletion of the Meckel syndrome genes MKS1 and MKS3.

The following MKS1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the MKS1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

Online Only Promotion

Up to 50% OFF! Starting
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***CloneID RefSeq Accession Definition **Vector *Turnaround time Price (USD) Select
OHu26205 NM_017777.3
Latest version!
Homo sapiens Meckel syndrome, type 1 (MKS1), transcript variant 1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
$234.50-$328.30
$469.00
OHu50508 NM_001321268.1
Latest version!
Homo sapiens Meckel syndrome, type 1 (MKS1), transcript variant 3, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 5-7 $223.30
$319.00
OHu26353 NM_001165927.1
Latest version!
Homo sapiens Meckel syndrome, type 1 (MKS1), transcript variant 2, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $307.30
$439.00
OHu50503 NM_001321269.1
Latest version!
Homo sapiens Meckel syndrome, type 1 (MKS1), transcript variant 4, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $307.30
$439.00
OHu50506 XM_005257485.3
Latest version!
Homo sapiens Meckel syndrome, type 1 (MKS1), transcript variant X7, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $223.30
$319.00
OHu50507 XM_006721965.2
Latest version!
Homo sapiens Meckel syndrome, type 1 (MKS1), transcript variant X9, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $223.30
$319.00
OHu68758 XM_011524957.2
Latest version!
Homo sapiens Meckel syndrome, type 1 (MKS1), transcript variant X1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $307.30
$439.00
OHu68759 XM_011524958.2
Latest version!
Homo sapiens Meckel syndrome, type 1 (MKS1), transcript variant X2, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $307.30
$439.00
OHu68760 XM_011524959.2
Latest version!
Homo sapiens Meckel syndrome, type 1 (MKS1), transcript variant X3, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $223.30
$319.00
OHu68761 XM_011524960.2
Latest version!
Homo sapiens Meckel syndrome, type 1 (MKS1), transcript variant X5, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $223.30
$319.00
OHu86960 XM_017024805.1
Latest version!
Homo sapiens Meckel syndrome, type 1 (MKS1), transcript variant X8, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $223.30
$319.00
OHu102306 NM_001330397.1
Latest version!
Homo sapiens Meckel syndrome, type 1 (MKS1), transcript variant 5, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 9-11 $223.30
$319.00
OHu86959 XM_017024804.1
Latest version!
Homo sapiens Meckel syndrome, type 1 (MKS1), transcript variant X6, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 9-11 $223.30
$319.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.


CloneID OHu26205
Clone ID Related Accession (Same CDS sequence) NM_017777.3
Accession Version NM_017777.3 Latest version! Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1680bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2017-05-07
Organism Homo sapiens(Human)
Product Meckel syndrome type 1 protein isoform 1
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DQ185029.1, AK000352.1 and BE327525.1. This sequence is a reference standard in the RefSeqGene project. On Sep 29, 2009 this sequence version replaced NM_017777.2. Transcript Variant: This variant (1) represents the longest transcript and encodes isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: DQ185029.1, SRR1803614.161680.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
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361
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481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
ATGGCGGAGA CCGTCTGGAG CACTGACACC GGGGAGGCAG TGTATCGCTC CCGGGACCCC 
GTGCGCAACT TGCGCCTCCG AGTCCACCTG CAAAGAATCA CATCAAGCAA CTTTCTTCAT
TATCAGCCTG CTGCCGAGCT CGGGAAGGAC CTCATAGACT TGGCCACTTT TAGGCCTCAG
CCAACTGCCA GTGGACACCG CCCAGAGGAA GACGAAGAGG AGGAGATTGT GATTGGGTGG
CAGGAGAAGC TCTTTAGCCA GTTTGAAGTA GATCTGTACC AAAATGAAAC AGCCTGTCAG
AGTCCTTTGG ATTATCAGTA CCGTCAGGAG ATCCTGAAGC TGGAGAATTC GGGTGGCAAG
AAAAACCGAC GAATCTTTAC CTACACTGAC TCTGATAGAT ACACCAATTT GGAGGAGCAC
TGTCAGAGAA TGACCACTGC AGCCAGCGAG GTGCCTTCAT TCTTGGTCGA GCGAATGGCA
AATGTCAGGC GTCGCCGGCA GGACAGGCGA GGGATGGAGG GCGGCATCCT CAAGTCACGC
ATCGTCACCT GGGAGCCCTC AGAAGAGTTT GTCAGGAACA ACCACGTCAT TAACACCCCT
CTTCAGACAA TGCACATCAT GGCAGACCTG GGGCCCTATA AAAAGCTTGG CTATAAGAAG
TATGAACATG TCCTGTGTAC TCTGAAGGTG GATAGCAATG GTGTGATCAC AGTAAAGCCT
GACTTCACGG GCCTCAAAGG ACCCTACAGG ATTGAGACGG AGGGGGAGAA GCAGGAGCTG
TGGAAATATA CGATCGACAA TGTTTCCCCC CACGCACAGC CGGAGGAGGA GGAGCGGGAA
CGGCGAGTGT TCAAGGATCT TTATGGCCGG CACAAGGAGT ATCTCAGCAG CCTCGTAGGC
ACCGACTTTG AGATGACTGT CCCAGGTGCC CTCCGGCTCT TTGTAAATGG AGAGGTCGTT
TCAGCCCAAG GCTATGAGTA TGACAATCTC TACGTCCACT TCTTTGTAGA ATTGCCAACT
GCTCACTGGT CAAGCCCAGC ATTCCAGCAG CTCTCAGGAG TAACACAGAC CTGCACCACC
AAGTCCCTGG CAATGGACAA GGTGGCTCAC TTCTCCTACC CATTCACGTT TGAAGCCTTC
TTCCTCCATG AGGATGAATC TTCTGATGCA CTCCCGGAGT GGCCTGTGCT CTACTGTGAG
GTCCTCTCGC TGGACTTCTG GCAGAGGTAC CGTGTGGAAG GCTATGGGGC TGTGGTGCTG
CCTGCCACTC CAGGCTCACA CACCCTGACA GTCTCCACGT GGAGACCTGT GGAGCTTGGC
ACGGTGGCTG AGCTGAGGAG GTTTTTCATT GGCGGTTCTC TGGAACTGGA GGACCTCTCC
TATGTACGGA TACCAGGATC CTTCAAGGGG GAACGCCTGA GCCGCTTTGG ACTCCGCACA
GAGACCACAG GCACTGTCAC CTTCCGCTTG CACTGTCTGC AGCAGTCCAG GGCCTTCATG
GAATCGAGCT CCCTTCAGAA AAGGATGCGG AGTGTGTTGG ACCGTCTGGA AGGGTTCAGC
CAGCAGAGTT CCATTCACAA TGTGCTAGAG GCCTTCCGTC GAGCCCGGCG CCGCATGCAG
GAGGCCCGGG AAAGCCTCCC GCAGGACCTA GTGAGCCCCT CTGGAACCCT GGTCTCCTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_060247.2
CDS76..1755
Misc Feature(1)1030..1551
Exon (1)1..155
Exon (2)156..265
Exon (3)266..336
Exon (4)337..492
Exon (5)493..590
Exon (6)591..719
Exon (7)720..824
Exon (8)825..933
Exon (9)934..990
Exon (10)991..1033
Exon (11)1034..1099
Exon (12)1100..1170
Exon (13)1171..1240
Exon (14)1241..1348
Exon (15)1349..1482
Exon (16)1483..1565
Exon (17)1566..1663
Exon (18)1664..2398
Translation

Target ORF information:

RefSeq Version NM_017777.3
Organism Homo sapiens(Human)
Definition Homo sapiens Meckel syndrome, type 1 (MKS1), transcript variant 1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_017777.3

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
ATGGCGGAGA CCGTCTGGAG CACTGACACC GGGGAGGCAG TGTATCGCTC CCGGGACCCC 
GTGCGCAACT TGCGCCTCCG AGTCCACCTG CAAAGAATCA CATCAAGCAA CTTTCTTCAT
TATCAGCCTG CTGCCGAGCT CGGGAAGGAC CTCATAGACT TGGCCACTTT TAGGCCTCAG
CCAACTGCCA GTGGACACCG CCCAGAGGAA GACGAAGAGG AGGAGATTGT GATTGGGTGG
CAGGAGAAGC TCTTTAGCCA GTTTGAAGTA GATCTGTACC AAAATGAAAC AGCCTGTCAG
AGTCCTTTGG ATTATCAGTA CCGTCAGGAG ATCCTGAAGC TGGAGAATTC GGGTGGCAAG
AAAAACCGAC GAATCTTTAC CTACACTGAC TCTGATAGAT ACACCAATTT GGAGGAGCAC
TGTCAGAGAA TGACCACTGC AGCCAGCGAG GTGCCTTCAT TCTTGGTCGA GCGAATGGCA
AATGTCAGGC GTCGCCGGCA GGACAGGCGA GGGATGGAGG GCGGCATCCT CAAGTCACGC
ATCGTCACCT GGGAGCCCTC AGAAGAGTTT GTCAGGAACA ACCACGTCAT TAACACCCCT
CTTCAGACAA TGCACATCAT GGCAGACCTG GGGCCCTATA AAAAGCTTGG CTATAAGAAG
TATGAACATG TCCTGTGTAC TCTGAAGGTG GATAGCAATG GTGTGATCAC AGTAAAGCCT
GACTTCACGG GCCTCAAAGG ACCCTACAGG ATTGAGACGG AGGGGGAGAA GCAGGAGCTG
TGGAAATATA CGATCGACAA TGTTTCCCCC CACGCACAGC CGGAGGAGGA GGAGCGGGAA
CGGCGAGTGT TCAAGGATCT TTATGGCCGG CACAAGGAGT ATCTCAGCAG CCTCGTAGGC
ACCGACTTTG AGATGACTGT CCCAGGTGCC CTCCGGCTCT TTGTAAATGG AGAGGTCGTT
TCAGCCCAAG GCTATGAGTA TGACAATCTC TACGTCCACT TCTTTGTAGA ATTGCCAACT
GCTCACTGGT CAAGCCCAGC ATTCCAGCAG CTCTCAGGAG TAACACAGAC CTGCACCACC
AAGTCCCTGG CAATGGACAA GGTGGCTCAC TTCTCCTACC CATTCACGTT TGAAGCCTTC
TTCCTCCATG AGGATGAATC TTCTGATGCA CTCCCGGAGT GGCCTGTGCT CTACTGTGAG
GTCCTCTCGC TGGACTTCTG GCAGAGGTAC CGTGTGGAAG GCTATGGGGC TGTGGTGCTG
CCTGCCACTC CAGGCTCACA CACCCTGACA GTCTCCACGT GGAGACCTGT GGAGCTTGGC
ACGGTGGCTG AGCTGAGGAG GTTTTTCATT GGCGGTTCTC TGGAACTGGA GGACCTCTCC
TATGTACGGA TACCAGGATC CTTCAAGGGG GAACGCCTGA GCCGCTTTGG ACTCCGCACA
GAGACCACAG GCACTGTCAC CTTCCGCTTG CACTGTCTGC AGCAGTCCAG GGCCTTCATG
GAATCGAGCT CCCTTCAGAA AAGGATGCGG AGTGTGTTGG ACCGTCTGGA AGGGTTCAGC
CAGCAGAGTT CCATTCACAA TGTGCTAGAG GCCTTCCGTC GAGCCCGGCG CCGCATGCAG
GAGGCCCGGG AAAGCCTCCC GCAGGACCTA GTGAGCCCCT CTGGAACCCT GGTCTCCTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu50508
Clone ID Related Accession (Same CDS sequence) NM_001321268.1
Accession Version NM_001321268.1 Latest version! Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1071bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2017-04-23
Organism Homo sapiens(Human)
Product Meckel syndrome type 1 protein isoform 3
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DQ185029.1, AK301020.1, AK000352.1 and BE327525.1. On Mar 19, 2016 this sequence version replaced XM_005257486.3. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK301020.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
ATGCACATCA TGGCAGACCT GGGGCCCTAT AAAAAGCTTG GCTATAAGAA GTATGAACAT 
GTCCTGTGTA CTCTGAAGGT GGATAGCAAT GGTGTGATCA CAGTAAAGCC TGACTTCACG
GGCCTCAAAG GACCCTACAG GATTGAGACG GAGGGGGAGA AGCAGGAGCT GTGGAAATAT
ACGATCGACA ATGTTTCCCC CCACGCACAG CCGGAGGAGG AGGAGCGGGA ACGGCGAGTG
TTCAAGGATC TTTATGGCCG GCACAAGGAG TATCTCAGCA GCCTCGTAGG CACCGACTTT
GAGATGACTG TCCCAGGTGC CCTCCGGCTC TTTGTAAATG GAGAGGTCGT TTCAGCCCAA
GGCTATGAGT ATGACAATCT CTACGTCCAC TTCTTTGTAG AATTGCCAAC TGCTCACTGG
TCAAGCCCAG CATTCCAGCA GCTCTCAGGA GTAACACAGA CCTGCACCAC CAAGTCCCTG
GCAATGGACA AGGTGGCTCA CTTCTCCTAC CCATTCACGT TTGAAGCCTT CTTCCTCCAT
GAGGATGAAT CTTCTGATGC ACTCCCGGAG TGGCCTGTGC TCTACTGTGA GGTCCTCTCG
CTGGACTTCT GGCAGAGGTA CCGTGTGGAA GGCTATGGGG CTGTGGTGCT GCCTGCCACT
CCAGGCTCAC ACACCCTGAC AGTCTCCACG TGGAGACCTG TGGAGCTTGG CACGGTGGCT
GAGCTGAGGA GGTTTTTCAT TGGCGGTTCT CTGGAACTGG AGGACCTCTC CTATGTACGG
ATACCAGGAT CCTTCAAGGG GGAACGCCTG AGCCGCTTTG GACTCCGCAC AGAGACCACA
GGCACTGTCA CCTTCCGCTT GCACTGTCTG CAGCAGTCCA GGGCCTTCAT GGAATCGAGC
TCCCTTCAGA AAAGGATGCG GAGTGTGTTG GACCGTCTGG AAGGGTTCAG CCAGCAGAGT
TCCATTCACA ATGTGCTAGA GGCCTTCCGT CGAGCCCGGC GCCGCATGCA GGAGGCCCGG
GAAAGCCTCC CGCAGGACCT AGTGAGCCCC TCTGGAACCC TGGTCTCCTA G

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001308197.1
CDS587..1657
Misc Feature(1)410..412
Misc Feature(2)932..1453
Exon (1)1..155
Exon (2)156..265
Exon (3)266..336
Exon (4)337..492
Exon (5)493..621
Exon (6)622..726
Exon (7)727..835
Exon (8)836..892
Exon (9)893..935
Exon (10)936..1001
Exon (11)1002..1072
Exon (12)1073..1142
Exon (13)1143..1250
Exon (14)1251..1384
Exon (15)1385..1467
Exon (16)1468..1565
Exon (17)1566..2300
Translation

Target ORF information:

RefSeq Version NM_001321268.1
Organism Homo sapiens(Human)
Definition Homo sapiens Meckel syndrome, type 1 (MKS1), transcript variant 3, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_001321268.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
ATGCACATCA TGGCAGACCT GGGGCCCTAT AAAAAGCTTG GCTATAAGAA GTATGAACAT 
GTCCTGTGTA CTCTGAAGGT GGATAGCAAT GGTGTGATCA CAGTAAAGCC TGACTTCACG
GGCCTCAAAG GACCCTACAG GATTGAGACG GAGGGGGAGA AGCAGGAGCT GTGGAAATAT
ACGATCGACA ATGTTTCCCC CCACGCACAG CCGGAGGAGG AGGAGCGGGA ACGGCGAGTG
TTCAAGGATC TTTATGGCCG GCACAAGGAG TATCTCAGCA GCCTCGTAGG CACCGACTTT
GAGATGACTG TCCCAGGTGC CCTCCGGCTC TTTGTAAATG GAGAGGTCGT TTCAGCCCAA
GGCTATGAGT ATGACAATCT CTACGTCCAC TTCTTTGTAG AATTGCCAAC TGCTCACTGG
TCAAGCCCAG CATTCCAGCA GCTCTCAGGA GTAACACAGA CCTGCACCAC CAAGTCCCTG
GCAATGGACA AGGTGGCTCA CTTCTCCTAC CCATTCACGT TTGAAGCCTT CTTCCTCCAT
GAGGATGAAT CTTCTGATGC ACTCCCGGAG TGGCCTGTGC TCTACTGTGA GGTCCTCTCG
CTGGACTTCT GGCAGAGGTA CCGTGTGGAA GGCTATGGGG CTGTGGTGCT GCCTGCCACT
CCAGGCTCAC ACACCCTGAC AGTCTCCACG TGGAGACCTG TGGAGCTTGG CACGGTGGCT
GAGCTGAGGA GGTTTTTCAT TGGCGGTTCT CTGGAACTGG AGGACCTCTC CTATGTACGG
ATACCAGGAT CCTTCAAGGG GGAACGCCTG AGCCGCTTTG GACTCCGCAC AGAGACCACA
GGCACTGTCA CCTTCCGCTT GCACTGTCTG CAGCAGTCCA GGGCCTTCAT GGAATCGAGC
TCCCTTCAGA AAAGGATGCG GAGTGTGTTG GACCGTCTGG AAGGGTTCAG CCAGCAGAGT
TCCATTCACA ATGTGCTAGA GGCCTTCCGT CGAGCCCGGC GCCGCATGCA GGAGGCCCGG
GAAAGCCTCC CGCAGGACCT AGTGAGCCCC TCTGGAACCC TGGTCTCCTA G

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu26353
Clone ID Related Accession (Same CDS sequence) NM_001165927.1
Accession Version NM_001165927.1 Latest version! Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1650bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2017-05-07
Organism Homo sapiens(Human)
Product Meckel syndrome type 1 protein isoform 2
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AK310815.1, AK000352.1 and BE327525.1. This sequence is a reference standard in the RefSeqGene project. Transcript Variant: This variant (2) represents use of an alternate promoter and 5' UTR and uses a distinct start codon, compared to variant 1. The resulting isoform (2) has a shorter and distinct N-terminus, compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK310815.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
ATGGCTGTGC CAGTTTCCTC ATTTGCACAG CGCACGAGGT CCCGCTTTCG AGTCCACCTG 
CAAAGAATCA CATCAAGCAA CTTTCTTCAT TATCAGCCTG CTGCCGAGCT CGGGAAGGAC
CTCATAGACT TGGCCACTTT TAGGCCTCAG CCAACTGCCA GTGGACACCG CCCAGAGGAA
GACGAAGAGG AGGAGATTGT GATTGGGTGG CAGGAGAAGC TCTTTAGCCA GTTTGAAGTA
GATCTGTACC AAAATGAAAC AGCCTGTCAG AGTCCTTTGG ATTATCAGTA CCGTCAGGAG
ATCCTGAAGC TGGAGAATTC GGGTGGCAAG AAAAACCGAC GAATCTTTAC CTACACTGAC
TCTGATAGAT ACACCAATTT GGAGGAGCAC TGTCAGAGAA TGACCACTGC AGCCAGCGAG
GTGCCTTCAT TCTTGGTCGA GCGAATGGCA AATGTCAGGC GTCGCCGGCA GGACAGGCGA
GGGATGGAGG GCGGCATCCT CAAGTCACGC ATCGTCACCT GGGAGCCCTC AGAAGAGTTT
GTCAGGAACA ACCACGTCAT TAACACCCCT CTTCAGACAA TGCACATCAT GGCAGACCTG
GGGCCCTATA AAAAGCTTGG CTATAAGAAG TATGAACATG TCCTGTGTAC TCTGAAGGTG
GATAGCAATG GTGTGATCAC AGTAAAGCCT GACTTCACGG GCCTCAAAGG ACCCTACAGG
ATTGAGACGG AGGGGGAGAA GCAGGAGCTG TGGAAATATA CGATCGACAA TGTTTCCCCC
CACGCACAGC CGGAGGAGGA GGAGCGGGAA CGGCGAGTGT TCAAGGATCT TTATGGCCGG
CACAAGGAGT ATCTCAGCAG CCTCGTAGGC ACCGACTTTG AGATGACTGT CCCAGGTGCC
CTCCGGCTCT TTGTAAATGG AGAGGTCGTT TCAGCCCAAG GCTATGAGTA TGACAATCTC
TACGTCCACT TCTTTGTAGA ATTGCCAACT GCTCACTGGT CAAGCCCAGC ATTCCAGCAG
CTCTCAGGAG TAACACAGAC CTGCACCACC AAGTCCCTGG CAATGGACAA GGTGGCTCAC
TTCTCCTACC CATTCACGTT TGAAGCCTTC TTCCTCCATG AGGATGAATC TTCTGATGCA
CTCCCGGAGT GGCCTGTGCT CTACTGTGAG GTCCTCTCGC TGGACTTCTG GCAGAGGTAC
CGTGTGGAAG GCTATGGGGC TGTGGTGCTG CCTGCCACTC CAGGCTCACA CACCCTGACA
GTCTCCACGT GGAGACCTGT GGAGCTTGGC ACGGTGGCTG AGCTGAGGAG GTTTTTCATT
GGCGGTTCTC TGGAACTGGA GGACCTCTCC TATGTACGGA TACCAGGATC CTTCAAGGGG
GAACGCCTGA GCCGCTTTGG ACTCCGCACA GAGACCACAG GCACTGTCAC CTTCCGCTTG
CACTGTCTGC AGCAGTCCAG GGCCTTCATG GAATCGAGCT CCCTTCAGAA AAGGATGCGG
AGTGTGTTGG ACCGTCTGGA AGGGTTCAGC CAGCAGAGTT CCATTCACAA TGTGCTAGAG
GCCTTCCGTC GAGCCCGGCG CCGCATGCAG GAGGCCCGGG AAAGCCTCCC GCAGGACCTA
GTGAGCCCCT CTGGAACCCT GGTCTCCTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001159399.1
CDS95..1744
Misc Feature(1)83..85
Misc Feature(2)1019..1540
Exon (1)1..144
Exon (2)145..254
Exon (3)255..325
Exon (4)326..481
Exon (5)482..579
Exon (6)580..708
Exon (7)709..813
Exon (8)814..922
Exon (9)923..979
Exon (10)980..1022
Exon (11)1023..1088
Exon (12)1089..1159
Exon (13)1160..1229
Exon (14)1230..1337
Exon (15)1338..1471
Exon (16)1472..1554
Exon (17)1555..1652
Exon (18)1653..2387
Translation

Target ORF information:

RefSeq Version NM_001165927.1
Organism Homo sapiens(Human)
Definition Homo sapiens Meckel syndrome, type 1 (MKS1), transcript variant 2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_001165927.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
ATGGCTGTGC CAGTTTCCTC ATTTGCACAG CGCACGAGGT CCCGCTTTCG AGTCCACCTG 
CAAAGAATCA CATCAAGCAA CTTTCTTCAT TATCAGCCTG CTGCCGAGCT CGGGAAGGAC
CTCATAGACT TGGCCACTTT TAGGCCTCAG CCAACTGCCA GTGGACACCG CCCAGAGGAA
GACGAAGAGG AGGAGATTGT GATTGGGTGG CAGGAGAAGC TCTTTAGCCA GTTTGAAGTA
GATCTGTACC AAAATGAAAC AGCCTGTCAG AGTCCTTTGG ATTATCAGTA CCGTCAGGAG
ATCCTGAAGC TGGAGAATTC GGGTGGCAAG AAAAACCGAC GAATCTTTAC CTACACTGAC
TCTGATAGAT ACACCAATTT GGAGGAGCAC TGTCAGAGAA TGACCACTGC AGCCAGCGAG
GTGCCTTCAT TCTTGGTCGA GCGAATGGCA AATGTCAGGC GTCGCCGGCA GGACAGGCGA
GGGATGGAGG GCGGCATCCT CAAGTCACGC ATCGTCACCT GGGAGCCCTC AGAAGAGTTT
GTCAGGAACA ACCACGTCAT TAACACCCCT CTTCAGACAA TGCACATCAT GGCAGACCTG
GGGCCCTATA AAAAGCTTGG CTATAAGAAG TATGAACATG TCCTGTGTAC TCTGAAGGTG
GATAGCAATG GTGTGATCAC AGTAAAGCCT GACTTCACGG GCCTCAAAGG ACCCTACAGG
ATTGAGACGG AGGGGGAGAA GCAGGAGCTG TGGAAATATA CGATCGACAA TGTTTCCCCC
CACGCACAGC CGGAGGAGGA GGAGCGGGAA CGGCGAGTGT TCAAGGATCT TTATGGCCGG
CACAAGGAGT ATCTCAGCAG CCTCGTAGGC ACCGACTTTG AGATGACTGT CCCAGGTGCC
CTCCGGCTCT TTGTAAATGG AGAGGTCGTT TCAGCCCAAG GCTATGAGTA TGACAATCTC
TACGTCCACT TCTTTGTAGA ATTGCCAACT GCTCACTGGT CAAGCCCAGC ATTCCAGCAG
CTCTCAGGAG TAACACAGAC CTGCACCACC AAGTCCCTGG CAATGGACAA GGTGGCTCAC
TTCTCCTACC CATTCACGTT TGAAGCCTTC TTCCTCCATG AGGATGAATC TTCTGATGCA
CTCCCGGAGT GGCCTGTGCT CTACTGTGAG GTCCTCTCGC TGGACTTCTG GCAGAGGTAC
CGTGTGGAAG GCTATGGGGC TGTGGTGCTG CCTGCCACTC CAGGCTCACA CACCCTGACA
GTCTCCACGT GGAGACCTGT GGAGCTTGGC ACGGTGGCTG AGCTGAGGAG GTTTTTCATT
GGCGGTTCTC TGGAACTGGA GGACCTCTCC TATGTACGGA TACCAGGATC CTTCAAGGGG
GAACGCCTGA GCCGCTTTGG ACTCCGCACA GAGACCACAG GCACTGTCAC CTTCCGCTTG
CACTGTCTGC AGCAGTCCAG GGCCTTCATG GAATCGAGCT CCCTTCAGAA AAGGATGCGG
AGTGTGTTGG ACCGTCTGGA AGGGTTCAGC CAGCAGAGTT CCATTCACAA TGTGCTAGAG
GCCTTCCGTC GAGCCCGGCG CCGCATGCAG GAGGCCCGGG AAAGCCTCCC GCAGGACCTA
GTGAGCCCCT CTGGAACCCT GGTCTCCTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu50503
Clone ID Related Accession (Same CDS sequence) NM_001321269.1
Accession Version NM_001321269.1 Latest version! Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1686bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2017-04-23
Organism Homo sapiens(Human)
Product Meckel syndrome type 1 protein isoform 4
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DQ185029.1, AK000352.1 and BE327525.1. On Mar 19, 2016 this sequence version replaced XM_005257483.3. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
ATGGCGGAGA CCGTCTGGAG CACTGACACC GGGGAGGCAG TGTATCGCTC CCGGGACCCC 
GTGCGCAACT TGCGCCTCCG AGTCCACCTG CAAAGAATCA CATCAAGCAA CTTTCTTCAT
TATCAGCCTG CTGCCGAGCT CGGGAAGGAC CTCATAGACT TGGCCACTTT TAGGCCTCAG
CCAACTGCCA GTGGACACCG CCCAGAGGAA GACGAAGAGG AGGAGATTGT GATTGGGTGG
CAGGAGAAGC TCTTTAGCCA GTTTGAAGTA GATCTGTACC AAAATGAAAC AGCCTGTCAG
AGTCCTTTGG ATTATCAGTA CCGTCAGGAG ATCCTGAAGC TGGAGAATTC GGGTGGCAAG
AAAAACCGAC GAATCTTTAC CTACACTGAC TCTGATAGAT ACACCAATTT GGAGGAGCAC
TGTCAGAGAA TGACCACTGC AGCCAGCGAG GTGCCTTCAT TCTTGGTCGA GCGAATGGCA
AATGTCAGGC GTCGCCGGCA GGACAGGCGA GGGATGGAGG GCGGCATCCT CAAGTCACGC
ATCGTCACCT GGGAGCCCTC AGAAGAGTTT GTCAGGAACA ACCACGTCAT TAACACCCCT
CTTCAGACAA TGCACATCAT GGCAGACCTG GGGCCCTATA AAAAGCTTGG CTATAAGAAG
TATGAACATG TCCTGTGTAC TCTGAAGGTG GATAGCAATG GTGTGATCAC AGTAAAGCCT
GACTTCACGG GCCTCAAAGG ACCCTACAGG ATTGAGACGG AGGGGGAGAA GCAGGAGCTG
TGGAAATATA CGATCGACAA TGTTTCCCCC CACGCACAGC CGGAGGAGGA GGAGCGGGAA
CGGCGAGTGT TCAAGGATCT TTATGGCCGG CACAAGGAGT ATCTCAGCAG CCTCGTAGGC
ACCGACTTTG AGATGACTGT CCCAGGTGCC CTCCGGCTCT TTGTAAATGG AGAGGTCGTT
TCAGCCCAAG GCTATGAGTA TGACAATCTC TACGTCCACT TCTTTGTAGA ATTGCCAACT
GCTCACTGGT CAAGCCCAGC ATTCCAGCAG CTCTCAGGAG TAACACAGAC CTGCACCACC
AAGTCCCTGG CAATGGACAA GGTGGCTCAC TTCTCCTACC CATTCACGTT TGAAGCCTTC
TTCCTCCATG AGGATGAATC TTCTGATGCA CTCCCGGAGT GGCCTGTGCT CTACTGTGAG
GTCCTCTCGC TGGACTTCTG GCAGAGGTAC CGTGTGGAAG GCTATGGGGC TGTGGTGCTG
CCTGCCACTC CAGGCTCACA CACCCTGACA GTCTCCACGT GGAGACCTGT GGAGCTTGGC
ACGGTGGCTG AGCTGAGGAG GTTTTTCATT GGCGGTTCTC TGGAACTGGA GGACCTCTCC
TATGTACGGA TACCAGGATC CTTCAAGGGC CTTCATGGAA TCGAGCTCCC TTCAGAAAAG
GATGCGGAGT GTGTTGGACC GTCTGGAAGG GTTCAGCCAG CAGAGTTCCA TTCACAATGT
GCTAGAGGCC TTCCGTCGAG CCCGGCGCCG CATGCAGGAG GCCCGGGAAA GCCTCCCGCA
GGACCTAGTG AGCCCCTCTG GAACCCTGGT CTCCTAGCTC ACAGCAGCCC TGGCCCACAG
TGCAAGAGGA CAAGATGGGG GATATCTGAG GCCAGTGCTC TCCTGCCTCT TCGTCTTTCT
GATTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001308198.1
CDS76..1761
Misc Feature(1)1030..1461
Exon (1)1..155
Exon (2)156..265
Exon (3)266..336
Exon (4)337..492
Exon (5)493..590
Exon (6)591..719
Exon (7)720..824
Exon (8)825..933
Exon (9)934..990
Exon (10)991..1033
Exon (11)1034..1099
Exon (12)1100..1170
Exon (13)1171..1240
Exon (14)1241..1348
Exon (15)1349..1482
Exon (16)1483..1580
Exon (17)1581..2315
Translation

Target ORF information:

RefSeq Version NM_001321269.1
Organism Homo sapiens(Human)
Definition Homo sapiens Meckel syndrome, type 1 (MKS1), transcript variant 4, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_001321269.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
ATGGCGGAGA CCGTCTGGAG CACTGACACC GGGGAGGCAG TGTATCGCTC CCGGGACCCC 
GTGCGCAACT TGCGCCTCCG AGTCCACCTG CAAAGAATCA CATCAAGCAA CTTTCTTCAT
TATCAGCCTG CTGCCGAGCT CGGGAAGGAC CTCATAGACT TGGCCACTTT TAGGCCTCAG
CCAACTGCCA GTGGACACCG CCCAGAGGAA GACGAAGAGG AGGAGATTGT GATTGGGTGG
CAGGAGAAGC TCTTTAGCCA GTTTGAAGTA GATCTGTACC AAAATGAAAC AGCCTGTCAG
AGTCCTTTGG ATTATCAGTA CCGTCAGGAG ATCCTGAAGC TGGAGAATTC GGGTGGCAAG
AAAAACCGAC GAATCTTTAC CTACACTGAC TCTGATAGAT ACACCAATTT GGAGGAGCAC
TGTCAGAGAA TGACCACTGC AGCCAGCGAG GTGCCTTCAT TCTTGGTCGA GCGAATGGCA
AATGTCAGGC GTCGCCGGCA GGACAGGCGA GGGATGGAGG GCGGCATCCT CAAGTCACGC
ATCGTCACCT GGGAGCCCTC AGAAGAGTTT GTCAGGAACA ACCACGTCAT TAACACCCCT
CTTCAGACAA TGCACATCAT GGCAGACCTG GGGCCCTATA AAAAGCTTGG CTATAAGAAG
TATGAACATG TCCTGTGTAC TCTGAAGGTG GATAGCAATG GTGTGATCAC AGTAAAGCCT
GACTTCACGG GCCTCAAAGG ACCCTACAGG ATTGAGACGG AGGGGGAGAA GCAGGAGCTG
TGGAAATATA CGATCGACAA TGTTTCCCCC CACGCACAGC CGGAGGAGGA GGAGCGGGAA
CGGCGAGTGT TCAAGGATCT TTATGGCCGG CACAAGGAGT ATCTCAGCAG CCTCGTAGGC
ACCGACTTTG AGATGACTGT CCCAGGTGCC CTCCGGCTCT TTGTAAATGG AGAGGTCGTT
TCAGCCCAAG GCTATGAGTA TGACAATCTC TACGTCCACT TCTTTGTAGA ATTGCCAACT
GCTCACTGGT CAAGCCCAGC ATTCCAGCAG CTCTCAGGAG TAACACAGAC CTGCACCACC
AAGTCCCTGG CAATGGACAA GGTGGCTCAC TTCTCCTACC CATTCACGTT TGAAGCCTTC
TTCCTCCATG AGGATGAATC TTCTGATGCA CTCCCGGAGT GGCCTGTGCT CTACTGTGAG
GTCCTCTCGC TGGACTTCTG GCAGAGGTAC CGTGTGGAAG GCTATGGGGC TGTGGTGCTG
CCTGCCACTC CAGGCTCACA CACCCTGACA GTCTCCACGT GGAGACCTGT GGAGCTTGGC
ACGGTGGCTG AGCTGAGGAG GTTTTTCATT GGCGGTTCTC TGGAACTGGA GGACCTCTCC
TATGTACGGA TACCAGGATC CTTCAAGGGC CTTCATGGAA TCGAGCTCCC TTCAGAAAAG
GATGCGGAGT GTGTTGGACC GTCTGGAAGG GTTCAGCCAG CAGAGTTCCA TTCACAATGT
GCTAGAGGCC TTCCGTCGAG CCCGGCGCCG CATGCAGGAG GCCCGGGAAA GCCTCCCGCA
GGACCTAGTG AGCCCCTCTG GAACCCTGGT CTCCTAGCTC ACAGCAGCCC TGGCCCACAG
TGCAAGAGGA CAAGATGGGG GATATCTGAG GCCAGTGCTC TCCTGCCTCT TCGTCTTTCT
GATTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu50506
Clone ID Related Accession (Same CDS sequence) XM_005257485.3
Accession Version XM_005257485.3 Latest version! Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1257bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2016-06-06
Organism Homo sapiens(Human)
Product Meckel syndrome type 1 protein isoform X7
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000017.11) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 12, 2015 this sequence version replaced XM_005257485.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
ATGACCACTG CAGCCAGCGA GGTGCCTTCA TTCTTGGTCG AGCGAATGGC AAATGTCAGG 
CGTCGCCGGC AGGACAGGCG AGGGATGGAG GGCGGCATCC TCAAGTCACG CATCGTCACC
TGGGAGCCCT CAGAAGAGTT TGTCAGGAAC AACCACGTCA TTAACACCCC TCTTCAGACA
ATGCACATCA TGGCAGACCT GGGGCCCTAT AAAAAGCTTG GCTATAAGAA GTATGAACAT
GTCCTGTGTA CTCTGAAGGT GGATAGCAAT GGTGTGATCA CAGTAAAGCC TGACTTCACG
GGCCTCAAAG GACCCTACAG GATTGAGACG GAGGGGGAGA AGCAGGAGCT GTGGAAATAT
ACGATCGACA ATGTTTCCCC CCACGCACAG CCGGAGGAGG AGGAGCGGGA ACGGCGAGTG
TTCAAGGATC TTTATGGCCG GCACAAGGAG TATCTCAGCA GCCTCGTAGG CACCGACTTT
GAGATGACTG TCCCAGGTGC CCTCCGGCTC TTTGTAAATG GAGAGGTCGT TTCAGCCCAA
GGCTATGAGT ATGACAATCT CTACGTCCAC TTCTTTGTAG AATTGCCAAC TGCTCACTGG
TCAAGCCCAG CATTCCAGCA GCTCTCAGGA GTAACACAGA CCTGCACCAC CAAGTCCCTG
GCAATGGACA AGGTGGCTCA CTTCTCCTAC CCATTCACGT TTGAAGCCTT CTTCCTCCAT
GAGGATGAAT CTTCTGATGC ACTCCCGGAG TGGCCTGTGC TCTACTGTGA GGTCCTCTCG
CTGGACTTCT GGCAGAGGTA CCGTGTGGAA GGCTATGGGG CTGTGGTGCT GCCTGCCACT
CCAGGCTCAC ACACCCTGAC AGTCTCCACG TGGAGACCTG TGGAGCTTGG CACGGTGGCT
GAGCTGAGGA GGTTTTTCAT TGGCGGTTCT CTGGAACTGG AGGACCTCTC CTATGTACGG
ATACCAGGAT CCTTCAAGGG CCTTCATGGA ATCGAGCTCC CTTCAGAAAA GGATGCGGAG
TGTGTTGGAC CGTCTGGAAG GGTTCAGCCA GCAGAGTTCC ATTCACAATG TGCTAGAGGC
CTTCCGTCGA GCCCGGCGCC GCATGCAGGA GGCCCGGGAA AGCCTCCCGC AGGACCTAGT
GAGCCCCTCT GGAACCCTGG TCTCCTAGCT CACAGCAGCC CTGGCCCACA GTGCAAGAGG
ACAAGATGGG GGATATCTGA GGCCAGTGCT CTCCTGCCTC TTCGTCTTTC TGATTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_005257542.1
CDS486..1742
Misc Feature(1)1011..1442
Translation

Target ORF information:

RefSeq Version XM_005257485.3
Organism Homo sapiens(Human)
Definition Homo sapiens Meckel syndrome, type 1 (MKS1), transcript variant X7, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_005257485.3

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
ATGACCACTG CAGCCAGCGA GGTGCCTTCA TTCTTGGTCG AGCGAATGGC AAATGTCAGG 
CGTCGCCGGC AGGACAGGCG AGGGATGGAG GGCGGCATCC TCAAGTCACG CATCGTCACC
TGGGAGCCCT CAGAAGAGTT TGTCAGGAAC AACCACGTCA TTAACACCCC TCTTCAGACA
ATGCACATCA TGGCAGACCT GGGGCCCTAT AAAAAGCTTG GCTATAAGAA GTATGAACAT
GTCCTGTGTA CTCTGAAGGT GGATAGCAAT GGTGTGATCA CAGTAAAGCC TGACTTCACG
GGCCTCAAAG GACCCTACAG GATTGAGACG GAGGGGGAGA AGCAGGAGCT GTGGAAATAT
ACGATCGACA ATGTTTCCCC CCACGCACAG CCGGAGGAGG AGGAGCGGGA ACGGCGAGTG
TTCAAGGATC TTTATGGCCG GCACAAGGAG TATCTCAGCA GCCTCGTAGG CACCGACTTT
GAGATGACTG TCCCAGGTGC CCTCCGGCTC TTTGTAAATG GAGAGGTCGT TTCAGCCCAA
GGCTATGAGT ATGACAATCT CTACGTCCAC TTCTTTGTAG AATTGCCAAC TGCTCACTGG
TCAAGCCCAG CATTCCAGCA GCTCTCAGGA GTAACACAGA CCTGCACCAC CAAGTCCCTG
GCAATGGACA AGGTGGCTCA CTTCTCCTAC CCATTCACGT TTGAAGCCTT CTTCCTCCAT
GAGGATGAAT CTTCTGATGC ACTCCCGGAG TGGCCTGTGC TCTACTGTGA GGTCCTCTCG
CTGGACTTCT GGCAGAGGTA CCGTGTGGAA GGCTATGGGG CTGTGGTGCT GCCTGCCACT
CCAGGCTCAC ACACCCTGAC AGTCTCCACG TGGAGACCTG TGGAGCTTGG CACGGTGGCT
GAGCTGAGGA GGTTTTTCAT TGGCGGTTCT CTGGAACTGG AGGACCTCTC CTATGTACGG
ATACCAGGAT CCTTCAAGGG CCTTCATGGA ATCGAGCTCC CTTCAGAAAA GGATGCGGAG
TGTGTTGGAC CGTCTGGAAG GGTTCAGCCA GCAGAGTTCC ATTCACAATG TGCTAGAGGC
CTTCCGTCGA GCCCGGCGCC GCATGCAGGA GGCCCGGGAA AGCCTCCCGC AGGACCTAGT
GAGCCCCTCT GGAACCCTGG TCTCCTAGCT CACAGCAGCC CTGGCCCACA GTGCAAGAGG
ACAAGATGGG GGATATCTGA GGCCAGTGCT CTCCTGCCTC TTCGTCTTTC TGATTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu50507
Clone ID Related Accession (Same CDS sequence) XM_006721965.2
Accession Version XM_006721965.2 Latest version! Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1077bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2016-06-06
Organism Homo sapiens(Human)
Product Meckel syndrome type 1 protein isoform X9
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000017.11) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 12, 2015 this sequence version replaced XM_006721965.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
ATGCACATCA TGGCAGACCT GGGGCCCTAT AAAAAGCTTG GCTATAAGAA GTATGAACAT 
GTCCTGTGTA CTCTGAAGGT GGATAGCAAT GGTGTGATCA CAGTAAAGCC TGACTTCACG
GGCCTCAAAG GACCCTACAG GATTGAGACG GAGGGGGAGA AGCAGGAGCT GTGGAAATAT
ACGATCGACA ATGTTTCCCC CCACGCACAG CCGGAGGAGG AGGAGCGGGA ACGGCGAGTG
TTCAAGGATC TTTATGGCCG GCACAAGGAG TATCTCAGCA GCCTCGTAGG CACCGACTTT
GAGATGACTG TCCCAGGTGC CCTCCGGCTC TTTGTAAATG GAGAGGTCGT TTCAGCCCAA
GGCTATGAGT ATGACAATCT CTACGTCCAC TTCTTTGTAG AATTGCCAAC TGCTCACTGG
TCAAGCCCAG CATTCCAGCA GCTCTCAGGA GTAACACAGA CCTGCACCAC CAAGTCCCTG
GCAATGGACA AGGTGGCTCA CTTCTCCTAC CCATTCACGT TTGAAGCCTT CTTCCTCCAT
GAGGATGAAT CTTCTGATGC ACTCCCGGAG TGGCCTGTGC TCTACTGTGA GGTCCTCTCG
CTGGACTTCT GGCAGAGGTA CCGTGTGGAA GGCTATGGGG CTGTGGTGCT GCCTGCCACT
CCAGGCTCAC ACACCCTGAC AGTCTCCACG TGGAGACCTG TGGAGCTTGG CACGGTGGCT
GAGCTGAGGA GGTTTTTCAT TGGCGGTTCT CTGGAACTGG AGGACCTCTC CTATGTACGG
ATACCAGGAT CCTTCAAGGG CCTTCATGGA ATCGAGCTCC CTTCAGAAAA GGATGCGGAG
TGTGTTGGAC CGTCTGGAAG GGTTCAGCCA GCAGAGTTCC ATTCACAATG TGCTAGAGGC
CTTCCGTCGA GCCCGGCGCC GCATGCAGGA GGCCCGGGAA AGCCTCCCGC AGGACCTAGT
GAGCCCCTCT GGAACCCTGG TCTCCTAGCT CACAGCAGCC CTGGCCCACA GTGCAAGAGG
ACAAGATGGG GGATATCTGA GGCCAGTGCT CTCCTGCCTC TTCGTCTTTC TGATTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_006722028.1
CDS519..1595
Misc Feature(1)864..1295
Translation

Target ORF information:

RefSeq Version XM_006721965.2
Organism Homo sapiens(Human)
Definition Homo sapiens Meckel syndrome, type 1 (MKS1), transcript variant X9, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_006721965.2

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
ATGCACATCA TGGCAGACCT GGGGCCCTAT AAAAAGCTTG GCTATAAGAA GTATGAACAT 
GTCCTGTGTA CTCTGAAGGT GGATAGCAAT GGTGTGATCA CAGTAAAGCC TGACTTCACG
GGCCTCAAAG GACCCTACAG GATTGAGACG GAGGGGGAGA AGCAGGAGCT GTGGAAATAT
ACGATCGACA ATGTTTCCCC CCACGCACAG CCGGAGGAGG AGGAGCGGGA ACGGCGAGTG
TTCAAGGATC TTTATGGCCG GCACAAGGAG TATCTCAGCA GCCTCGTAGG CACCGACTTT
GAGATGACTG TCCCAGGTGC CCTCCGGCTC TTTGTAAATG GAGAGGTCGT TTCAGCCCAA
GGCTATGAGT ATGACAATCT CTACGTCCAC TTCTTTGTAG AATTGCCAAC TGCTCACTGG
TCAAGCCCAG CATTCCAGCA GCTCTCAGGA GTAACACAGA CCTGCACCAC CAAGTCCCTG
GCAATGGACA AGGTGGCTCA CTTCTCCTAC CCATTCACGT TTGAAGCCTT CTTCCTCCAT
GAGGATGAAT CTTCTGATGC ACTCCCGGAG TGGCCTGTGC TCTACTGTGA GGTCCTCTCG
CTGGACTTCT GGCAGAGGTA CCGTGTGGAA GGCTATGGGG CTGTGGTGCT GCCTGCCACT
CCAGGCTCAC ACACCCTGAC AGTCTCCACG TGGAGACCTG TGGAGCTTGG CACGGTGGCT
GAGCTGAGGA GGTTTTTCAT TGGCGGTTCT CTGGAACTGG AGGACCTCTC CTATGTACGG
ATACCAGGAT CCTTCAAGGG CCTTCATGGA ATCGAGCTCC CTTCAGAAAA GGATGCGGAG
TGTGTTGGAC CGTCTGGAAG GGTTCAGCCA GCAGAGTTCC ATTCACAATG TGCTAGAGGC
CTTCCGTCGA GCCCGGCGCC GCATGCAGGA GGCCCGGGAA AGCCTCCCGC AGGACCTAGT
GAGCCCCTCT GGAACCCTGG TCTCCTAGCT CACAGCAGCC CTGGCCCACA GTGCAAGAGG
ACAAGATGGG GGATATCTGA GGCCAGTGCT CTCCTGCCTC TTCGTCTTTC TGATTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu68758
Clone ID Related Accession (Same CDS sequence) XM_011524957.2
Accession Version XM_011524957.2 Latest version! Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1695bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2016-06-06
Organism Homo sapiens(Human)
Product Meckel syndrome type 1 protein isoform X1
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000017.11) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011524957.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
ATGGCGGAGA CCGTCTGGAG CACTGACACC GGGGAGGCAG TGTATCGCTC CCGGGACCCC 
GTGCGCAACT TGCGCCTCCG AGTCCACCTG CAAAGAATCA CATCAAGCAA CTTTCTTCAT
TATCAGCCTG CTGCCGAGCT CGGGAAGGAC CTCATAGACT TGGCCACTTT TAGGCCTCAG
CCAACTGCCA TCTTTGGAGG TGGACACCGC CCAGAGGAAG ACGAAGAGGA GGAGATTGTG
ATTGGGTGGC AGGAGAAGCT CTTTAGCCAG TTTGAAGTAG ATCTGTACCA AAATGAAACA
GCCTGTCAGA GTCCTTTGGA TTATCAGTAC CGTCAGGAGA TCCTGAAGCT GGAGAATTCG
GGTGGCAAGA AAAACCGACG AATCTTTACC TACACTGACT CTGATAGATA CACCAATTTG
GAGGAGCACT GTCAGAGAAT GACCACTGCA GCCAGCGAGG TGCCTTCATT CTTGGTCGAG
CGAATGGCAA ATGTCAGGCG TCGCCGGCAG GACAGGCGAG GGATGGAGGG CGGCATCCTC
AAGTCACGCA TCGTCACCTG GGAGCCCTCA GAAGAGTTTG TCAGGAACAA CCACGTCATT
AACACCCCTC TTCAGACAAT GCACATCATG GCAGACCTGG GGCCCTATAA AAAGCTTGGC
TATAAGAAGT ATGAACATGT CCTGTGTACT CTGAAGGTGG ATAGCAATGG TGTGATCACA
GTAAAGCCTG ACTTCACGGG CCTCAAAGGA CCCTACAGGA TTGAGACGGA GGGGGAGAAG
CAGGAGCTGT GGAAATATAC GATCGACAAT GTTTCCCCCC ACGCACAGCC GGAGGAGGAG
GAGCGGGAAC GGCGAGTGTT CAAGGATCTT TATGGCCGGC ACAAGGAGTA TCTCAGCAGC
CTCGTAGGCA CCGACTTTGA GATGACTGTC CCAGGTGCCC TCCGGCTCTT TGTAAATGGA
GAGGTCGTTT CAGCCCAAGG CTATGAGTAT GACAATCTCT ACGTCCACTT CTTTGTAGAA
TTGCCAACTG CTCACTGGTC AAGCCCAGCA TTCCAGCAGC TCTCAGGAGT AACACAGACC
TGCACCACCA AGTCCCTGGC AATGGACAAG GTGGCTCACT TCTCCTACCC ATTCACGTTT
GAAGCCTTCT TCCTCCATGA GGATGAATCT TCTGATGCAC TCCCGGAGTG GCCTGTGCTC
TACTGTGAGG TCCTCTCGCT GGACTTCTGG CAGAGGTACC GTGTGGAAGG CTATGGGGCT
GTGGTGCTGC CTGCCACTCC AGGCTCACAC ACCCTGACAG TCTCCACGTG GAGACCTGTG
GAGCTTGGCA CGGTGGCTGA GCTGAGGAGG TTTTTCATTG GCGGTTCTCT GGAACTGGAG
GACCTCTCCT ATGTACGGAT ACCAGGATCC TTCAAGGGCC TTCATGGAAT CGAGCTCCCT
TCAGAAAAGG ATGCGGAGTG TGTTGGACCG TCTGGAAGGG TTCAGCCAGC AGAGTTCCAT
TCACAATGTG CTAGAGGCCT TCCGTCGAGC CCGGCGCCGC ATGCAGGAGG CCCGGGAAAG
CCTCCCGCAG GACCTAGTGA GCCCCTCTGG AACCCTGGTC TCCTAGCTCA CAGCAGCCCT
GGCCCACAGT GCAAGAGGAC AAGATGGGGG ATATCTGAGG CCAGTGCTCT CCTGCCTCTT
CGTCTTTCTG ATTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_011523259.1
CDS47..1741
Misc Feature(1)1010..1441
Translation

Target ORF information:

RefSeq Version XM_011524957.2
Organism Homo sapiens(Human)
Definition Homo sapiens Meckel syndrome, type 1 (MKS1), transcript variant X1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_011524957.2

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
ATGGCGGAGA CCGTCTGGAG CACTGACACC GGGGAGGCAG TGTATCGCTC CCGGGACCCC 
GTGCGCAACT TGCGCCTCCG AGTCCACCTG CAAAGAATCA CATCAAGCAA CTTTCTTCAT
TATCAGCCTG CTGCCGAGCT CGGGAAGGAC CTCATAGACT TGGCCACTTT TAGGCCTCAG
CCAACTGCCA TCTTTGGAGG TGGACACCGC CCAGAGGAAG ACGAAGAGGA GGAGATTGTG
ATTGGGTGGC AGGAGAAGCT CTTTAGCCAG TTTGAAGTAG ATCTGTACCA AAATGAAACA
GCCTGTCAGA GTCCTTTGGA TTATCAGTAC CGTCAGGAGA TCCTGAAGCT GGAGAATTCG
GGTGGCAAGA AAAACCGACG AATCTTTACC TACACTGACT CTGATAGATA CACCAATTTG
GAGGAGCACT GTCAGAGAAT GACCACTGCA GCCAGCGAGG TGCCTTCATT CTTGGTCGAG
CGAATGGCAA ATGTCAGGCG TCGCCGGCAG GACAGGCGAG GGATGGAGGG CGGCATCCTC
AAGTCACGCA TCGTCACCTG GGAGCCCTCA GAAGAGTTTG TCAGGAACAA CCACGTCATT
AACACCCCTC TTCAGACAAT GCACATCATG GCAGACCTGG GGCCCTATAA AAAGCTTGGC
TATAAGAAGT ATGAACATGT CCTGTGTACT CTGAAGGTGG ATAGCAATGG TGTGATCACA
GTAAAGCCTG ACTTCACGGG CCTCAAAGGA CCCTACAGGA TTGAGACGGA GGGGGAGAAG
CAGGAGCTGT GGAAATATAC GATCGACAAT GTTTCCCCCC ACGCACAGCC GGAGGAGGAG
GAGCGGGAAC GGCGAGTGTT CAAGGATCTT TATGGCCGGC ACAAGGAGTA TCTCAGCAGC
CTCGTAGGCA CCGACTTTGA GATGACTGTC CCAGGTGCCC TCCGGCTCTT TGTAAATGGA
GAGGTCGTTT CAGCCCAAGG CTATGAGTAT GACAATCTCT ACGTCCACTT CTTTGTAGAA
TTGCCAACTG CTCACTGGTC AAGCCCAGCA TTCCAGCAGC TCTCAGGAGT AACACAGACC
TGCACCACCA AGTCCCTGGC AATGGACAAG GTGGCTCACT TCTCCTACCC ATTCACGTTT
GAAGCCTTCT TCCTCCATGA GGATGAATCT TCTGATGCAC TCCCGGAGTG GCCTGTGCTC
TACTGTGAGG TCCTCTCGCT GGACTTCTGG CAGAGGTACC GTGTGGAAGG CTATGGGGCT
GTGGTGCTGC CTGCCACTCC AGGCTCACAC ACCCTGACAG TCTCCACGTG GAGACCTGTG
GAGCTTGGCA CGGTGGCTGA GCTGAGGAGG TTTTTCATTG GCGGTTCTCT GGAACTGGAG
GACCTCTCCT ATGTACGGAT ACCAGGATCC TTCAAGGGCC TTCATGGAAT CGAGCTCCCT
TCAGAAAAGG ATGCGGAGTG TGTTGGACCG TCTGGAAGGG TTCAGCCAGC AGAGTTCCAT
TCACAATGTG CTAGAGGCCT TCCGTCGAGC CCGGCGCCGC ATGCAGGAGG CCCGGGAAAG
CCTCCCGCAG GACCTAGTGA GCCCCTCTGG AACCCTGGTC TCCTAGCTCA CAGCAGCCCT
GGCCCACAGT GCAAGAGGAC AAGATGGGGG ATATCTGAGG CCAGTGCTCT CCTGCCTCTT
CGTCTTTCTG ATTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu68759
Clone ID Related Accession (Same CDS sequence) XM_011524958.2
Accession Version XM_011524958.2 Latest version! Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1689bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2016-06-06
Organism Homo sapiens(Human)
Product Meckel syndrome type 1 protein isoform X2
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000017.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011524958.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
ATGGCGGAGA CCGTCTGGAG CACTGACACC GGGGAGGCAG TGTATCGCTC CCGGGACCCC 
GTGCGCAACT TGCGCCTCCG AGTCCACCTG CAAAGAATCA CATCAAGCAA CTTTCTTCAT
TATCAGCCTG CTGCCGAGCT CGGGAAGGAC CTCATAGACT TGGCCACTTT TAGGCCTCAG
CCAACTGCCA TCTTTGGAGG TGGACACCGC CCAGAGGAAG ACGAAGAGGA GGAGATTGTG
ATTGGGTGGC AGGAGAAGCT CTTTAGCCAG TTTGAAGTAG ATCTGTACCA AAATGAAACA
GCCTGTCAGA GTCCTTTGGA TTATCAGTAC CGTCAGGAGA TCCTGAAGCT GGAGAATTCG
GGTGGCAAGA AAAACCGACG AATCTTTACC TACACTGACT CTGATAGATA CACCAATTTG
GAGGAGCACT GTCAGAGAAT GACCACTGCA GCCAGCGAGG TGCCTTCATT CTTGGTCGAG
CGAATGGCAA ATGTCAGGCG TCGCCGGCAG GACAGGCGAG GGATGGAGGG CGGCATCCTC
AAGTCACGCA TCGTCACCTG GGAGCCCTCA GAAGAGTTTG TCAGGAACAA CCACGTCATT
AACACCCCTC TTCAGACAAT GCACATCATG GCAGACCTGG GGCCCTATAA AAAGCTTGGC
TATAAGAAGT ATGAACATGT CCTGTGTACT CTGAAGGTGG ATAGCAATGG TGTGATCACA
GTAAAGCCTG ACTTCACGGG CCTCAAAGGA CCCTACAGGA TTGAGACGGA GGGGGAGAAG
CAGGAGCTGT GGAAATATAC GATCGACAAT GTTTCCCCCC ACGCACAGCC GGAGGAGGAG
GAGCGGGAAC GGCGAGTGTT CAAGGATCTT TATGGCCGGC ACAAGGAGTA TCTCAGCAGC
CTCGTAGGCA CCGACTTTGA GATGACTGTC CCAGGTGCCC TCCGGCTCTT TGTAAATGGA
GAGGTCGTTT CAGCCCAAGG CTATGAGTAT GACAATCTCT ACGTCCACTT CTTTGTAGAA
TTGCCAACTG CTCACTGGTC AAGCCCAGCA TTCCAGCAGC TCTCAGGAGT AACACAGACC
TGCACCACCA AGTCCCTGGC AATGGACAAG GTGGCTCACT TCTCCTACCC ATTCACGTTT
GAAGCCTTCT TCCTCCATGA GGATGAATCT TCTGATGCAC TCCCGGAGTG GCCTGTGCTC
TACTGTGAGG TCCTCTCGCT GGACTTCTGG CAGAGGTACC GTGTGGAAGG CTATGGGGCT
GTGGTGCTGC CTGCCACTCC AGGCTCACAC ACCCTGACAG TCTCCACGTG GAGACCTGTG
GAGCTTGGCA CGGTGGCTGA GCTGAGGAGG TTTTTCATTG GCGGTTCTCT GGAACTGGAG
GACCTCTCCT ATGTACGGAT ACCAGGATCC TTCAAGGGGG AACGCCTGAG CCGCTTTGGA
CTCCGCACAG AGACCACAGG CACTGTCACC TTCCGCTTGC ACTGTCTGCA GCAGTCCAGG
GCCTTCATGG AATCGAGCTC CCTTCAGAAA AGGATGCGGA GTGTGTTGGA CCGTCTGGAA
GGGTTCAGCC AGCAGAGTTC CATTCACAAT GTGCTAGAGG CCTTCCGTCG AGCCCGGCGC
CGCATGCAGG AGGCCCGGGA AAGCCTCCCG CAGGACCTAG TGAGCCCCTC TGGAACCCTG
GTCTCCTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_011523260.1
CDS47..1735
Misc Feature(1)1010..1531
Translation

Target ORF information:

RefSeq Version XM_011524958.2
Organism Homo sapiens(Human)
Definition Homo sapiens Meckel syndrome, type 1 (MKS1), transcript variant X2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_011524958.2

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
ATGGCGGAGA CCGTCTGGAG CACTGACACC GGGGAGGCAG TGTATCGCTC CCGGGACCCC 
GTGCGCAACT TGCGCCTCCG AGTCCACCTG CAAAGAATCA CATCAAGCAA CTTTCTTCAT
TATCAGCCTG CTGCCGAGCT CGGGAAGGAC CTCATAGACT TGGCCACTTT TAGGCCTCAG
CCAACTGCCA TCTTTGGAGG TGGACACCGC CCAGAGGAAG ACGAAGAGGA GGAGATTGTG
ATTGGGTGGC AGGAGAAGCT CTTTAGCCAG TTTGAAGTAG ATCTGTACCA AAATGAAACA
GCCTGTCAGA GTCCTTTGGA TTATCAGTAC CGTCAGGAGA TCCTGAAGCT GGAGAATTCG
GGTGGCAAGA AAAACCGACG AATCTTTACC TACACTGACT CTGATAGATA CACCAATTTG
GAGGAGCACT GTCAGAGAAT GACCACTGCA GCCAGCGAGG TGCCTTCATT CTTGGTCGAG
CGAATGGCAA ATGTCAGGCG TCGCCGGCAG GACAGGCGAG GGATGGAGGG CGGCATCCTC
AAGTCACGCA TCGTCACCTG GGAGCCCTCA GAAGAGTTTG TCAGGAACAA CCACGTCATT
AACACCCCTC TTCAGACAAT GCACATCATG GCAGACCTGG GGCCCTATAA AAAGCTTGGC
TATAAGAAGT ATGAACATGT CCTGTGTACT CTGAAGGTGG ATAGCAATGG TGTGATCACA
GTAAAGCCTG ACTTCACGGG CCTCAAAGGA CCCTACAGGA TTGAGACGGA GGGGGAGAAG
CAGGAGCTGT GGAAATATAC GATCGACAAT GTTTCCCCCC ACGCACAGCC GGAGGAGGAG
GAGCGGGAAC GGCGAGTGTT CAAGGATCTT TATGGCCGGC ACAAGGAGTA TCTCAGCAGC
CTCGTAGGCA CCGACTTTGA GATGACTGTC CCAGGTGCCC TCCGGCTCTT TGTAAATGGA
GAGGTCGTTT CAGCCCAAGG CTATGAGTAT GACAATCTCT ACGTCCACTT CTTTGTAGAA
TTGCCAACTG CTCACTGGTC AAGCCCAGCA TTCCAGCAGC TCTCAGGAGT AACACAGACC
TGCACCACCA AGTCCCTGGC AATGGACAAG GTGGCTCACT TCTCCTACCC ATTCACGTTT
GAAGCCTTCT TCCTCCATGA GGATGAATCT TCTGATGCAC TCCCGGAGTG GCCTGTGCTC
TACTGTGAGG TCCTCTCGCT GGACTTCTGG CAGAGGTACC GTGTGGAAGG CTATGGGGCT
GTGGTGCTGC CTGCCACTCC AGGCTCACAC ACCCTGACAG TCTCCACGTG GAGACCTGTG
GAGCTTGGCA CGGTGGCTGA GCTGAGGAGG TTTTTCATTG GCGGTTCTCT GGAACTGGAG
GACCTCTCCT ATGTACGGAT ACCAGGATCC TTCAAGGGGG AACGCCTGAG CCGCTTTGGA
CTCCGCACAG AGACCACAGG CACTGTCACC TTCCGCTTGC ACTGTCTGCA GCAGTCCAGG
GCCTTCATGG AATCGAGCTC CCTTCAGAAA AGGATGCGGA GTGTGTTGGA CCGTCTGGAA
GGGTTCAGCC AGCAGAGTTC CATTCACAAT GTGCTAGAGG CCTTCCGTCG AGCCCGGCGC
CGCATGCAGG AGGCCCGGGA AAGCCTCCCG CAGGACCTAG TGAGCCCCTC TGGAACCCTG
GTCTCCTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu68760
Clone ID Related Accession (Same CDS sequence) XM_011524959.2
Accession Version XM_011524959.2 Latest version! Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1380bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2016-06-06
Organism Homo sapiens(Human)
Product Meckel syndrome type 1 protein isoform X3
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000017.11) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011524959.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
ATGGCGGAGA CCGTCTGGAG CACTGACACC GGGGAGGCAG TGTATCGCTC CCGGGACCCC 
GTGCGCAACT TGCGCCTCCG AGTCCACCTG CAAAGAATCA CATCAAGCAA CTTTCTTCAT
TATCAGCCTG CTGCCGAGCT CGGGAAGGAC CTCATAGACT TGGCCACTTT TAGGCCTCAG
CCAACTGCCA TCTTTGGAGG TGGACACCGC CCAGAGGAAG ACGAAGAGGA GGAGATTGTG
ATTGGGTGGC AGGAGAAGCT CTTTAGCCAG TTTGAAGTAG ATCTGTACCA AAATGAAACA
GCCTGTCAGA GTCCTTTGGA TTATCAGTAC CGTCAGGAGA TCCTGAAGCT GGAGAATTCG
GGTGGCAAGA AAAACCGACG AATCTTTACC TACACTGACT CTGATAGATA CACCAATTTG
GAGGAGCACT GTCAGAGAAT GACCACTGCA GCCAGCGAGG TGCCTTCATT CTTGGTCGAG
CGAATGGCAA ATGTCAGGCG TCGCCGGCAG GACAGGCGAG GGATGGAGGG CGGCATCCTC
AAGTCACGCA TCGTCACCTG GGAGCCCTCA GAAGAGTTTG TCAGGAACAA CCACGTCATT
AACACCCCTC TTCAGACAAT GCACATCATG GCAGACCTGG GGCCCTATAA AAAGCTTGGC
TATAAGAAGT ATGAACATGT CCTGTGTACT CTGAAGGTGG ATAGCAATGG TGTGATCACA
GTAAAGCCTG ACTTCACGGG CCTCAAAGGA CCCTACAGGA TTGAGACGGA GGGGGAGAAG
CAGGAGCTGT GGAAATATAC GATCGACAAT GTTTCCCCCC ACGCACAGCC GGAGGAGGAG
GAGCGGGAAC GGCGAGTGTT CAAGGATCTT TATGGCCGGC ACAAGGAGTA TCTCAGCAGC
CTCGTAGGCA CCGACTTTGA GATGACTGTC CCAGGTGCCC TCCGGCTCTT TGTAAATGGA
GAGGTCGTTT CAGCCCAAGG CTATGAGTAT GACAATCTCT ACGTCCACTT CTTTGTAGAA
TTGCCAACTG CTCACTGGTC AAGCCCAGCA TTCCAGCAGC TCTCAGGAGT AACACAGACC
TGCACCACCA AGTCCCTGGC AATGGACAAG GTGGCTCACT TCTCCTACCC ATTCACGTTT
GAAGCCTTCT TCCTCCATGA GGATGAATCT TCTGATGCAC TCCCGGAGTG GCCTGTGCTC
TACTGTGAGG TCCTCTCGCT GGACTTCTGG CAGAGGTACC GTGTGGAAGG CTATGGGGCT
GTGGTGCTGC CTGCCACTCC AGGGCCTTCA TGGAATCGAG CTCCCTTCAG AAAAGGATGC
GGAGTGTGTT GGACCGTCTG GAAGGGTTCA GCCAGCAGAG TTCCATTCAC AATGTGCTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_011523261.1
CDS46..1425
Misc Feature(1)1009..>1329
Translation

Target ORF information:

RefSeq Version XM_011524959.2
Organism Homo sapiens(Human)
Definition Homo sapiens Meckel syndrome, type 1 (MKS1), transcript variant X3, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_011524959.2

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
ATGGCGGAGA CCGTCTGGAG CACTGACACC GGGGAGGCAG TGTATCGCTC CCGGGACCCC 
GTGCGCAACT TGCGCCTCCG AGTCCACCTG CAAAGAATCA CATCAAGCAA CTTTCTTCAT
TATCAGCCTG CTGCCGAGCT CGGGAAGGAC CTCATAGACT TGGCCACTTT TAGGCCTCAG
CCAACTGCCA TCTTTGGAGG TGGACACCGC CCAGAGGAAG ACGAAGAGGA GGAGATTGTG
ATTGGGTGGC AGGAGAAGCT CTTTAGCCAG TTTGAAGTAG ATCTGTACCA AAATGAAACA
GCCTGTCAGA GTCCTTTGGA TTATCAGTAC CGTCAGGAGA TCCTGAAGCT GGAGAATTCG
GGTGGCAAGA AAAACCGACG AATCTTTACC TACACTGACT CTGATAGATA CACCAATTTG
GAGGAGCACT GTCAGAGAAT GACCACTGCA GCCAGCGAGG TGCCTTCATT CTTGGTCGAG
CGAATGGCAA ATGTCAGGCG TCGCCGGCAG GACAGGCGAG GGATGGAGGG CGGCATCCTC
AAGTCACGCA TCGTCACCTG GGAGCCCTCA GAAGAGTTTG TCAGGAACAA CCACGTCATT
AACACCCCTC TTCAGACAAT GCACATCATG GCAGACCTGG GGCCCTATAA AAAGCTTGGC
TATAAGAAGT ATGAACATGT CCTGTGTACT CTGAAGGTGG ATAGCAATGG TGTGATCACA
GTAAAGCCTG ACTTCACGGG CCTCAAAGGA CCCTACAGGA TTGAGACGGA GGGGGAGAAG
CAGGAGCTGT GGAAATATAC GATCGACAAT GTTTCCCCCC ACGCACAGCC GGAGGAGGAG
GAGCGGGAAC GGCGAGTGTT CAAGGATCTT TATGGCCGGC ACAAGGAGTA TCTCAGCAGC
CTCGTAGGCA CCGACTTTGA GATGACTGTC CCAGGTGCCC TCCGGCTCTT TGTAAATGGA
GAGGTCGTTT CAGCCCAAGG CTATGAGTAT GACAATCTCT ACGTCCACTT CTTTGTAGAA
TTGCCAACTG CTCACTGGTC AAGCCCAGCA TTCCAGCAGC TCTCAGGAGT AACACAGACC
TGCACCACCA AGTCCCTGGC AATGGACAAG GTGGCTCACT TCTCCTACCC ATTCACGTTT
GAAGCCTTCT TCCTCCATGA GGATGAATCT TCTGATGCAC TCCCGGAGTG GCCTGTGCTC
TACTGTGAGG TCCTCTCGCT GGACTTCTGG CAGAGGTACC GTGTGGAAGG CTATGGGGCT
GTGGTGCTGC CTGCCACTCC AGGGCCTTCA TGGAATCGAG CTCCCTTCAG AAAAGGATGC
GGAGTGTGTT GGACCGTCTG GAAGGGTTCA GCCAGCAGAG TTCCATTCAC AATGTGCTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu68761
Clone ID Related Accession (Same CDS sequence) XM_011524960.2
Accession Version XM_011524960.2 Latest version! Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1308bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2016-06-06
Organism Homo sapiens(Human)
Product Meckel syndrome type 1 protein isoform X5
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000017.11) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011524960.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
ATGGCGGAGA CCGTCTGGAG CACTGACACC GGGGAGGCAG TGTATCGCTC CCGGGACCCC 
GTGCGCAACT TGCGCCTCCG AGTCCACCTG CAAAGAATCA CATCAAGCAA CTTTCTTCAT
TATCAGCCTG CTGCCGAGCT CGGGAAGGAC CTCATAGACT TGGCCACTTT TAGGCCTCAG
CCAACTGCCA TCTTTGGAGG TGGACACCGC CCAGAGGAAG ACGAAGAGGA GGAGATTGTG
ATTGGGTGGC AGGAGAAGCT CTTTAGCCAG TTTGAAGTAG ATCTGTACCA AAATGAAACA
GCCTGTCAGA GTCCTTTGGA TTATCAGTAC CGTCAGGAGA TCCTGAAGCT GGAGAATTCG
GGTGGCAAGA AAAACCGACG AATCTTTACC TACACTGACT CTGATAGATA CACCAATTTG
GAGGAGCACT GTCAGAGAAT GACCACTGCA GCCAGCGAGG TGCCTTCATT CTTGGTCGAG
CGAATGGCAA ATGTCAGGCG TCGCCGGCAG GACAGGCGAG GGATGGAGGG CGGCATCCTC
AAGTCACGCA TCGTCACCTG GGAGCCCTCA GAAGAGTTTG TCAGGAACAA CCACGTCATT
AACACCCCTC TTCAGACAAT GCACATCATG GCAGACCTGG GGCCCTATAA AAAGCTTGGC
TATAAGAAGT ATGAACATGT CCTGTGTACT CTGAAGGTGG ATAGCAATGG TGTGATCACA
GTAAAGCCTG ACTTCACGGG CCTCAAAGGA CCCTACAGGA TTGAGACGGA GGGGGAGAAG
CAGGAGCTGT GGAAATATAC GATCGACAAT GTTTCCCCCC ACGCACAGCC GGAGGAGGAG
GAGCGGGAAC GGCGAGTGTT CAAGGATCTT TATGGCCGGC ACAAGGAGTA TCTCAGCAGC
CTCGTAGGCA CCGACTTTGA GATGACTGTC CCAGGTGCCC TCCGGCTCTT TGTAAATGGA
GAGGTCGTTT CAGCCCAAGG CTATGAGTAT GACAATCTCT ACGTCCACTT CTTTGTAGAA
TTGCCAACTG CTCACTGGTC AAGCCCAGCA TTCCAGCAGC TCTCAGGAGT AACACAGACC
TGCACCACCA AGTCCCTGGC AATGGACAAG GTGGCTCACT TCTCCTACCC ATTCACGTTT
GAAGCCTTCT TCCTCCATGA GGATGAATCT TCTGATGCAC TCCCGGAGTG GCCTGTGCTC
TACTGTGAGG TCCTCTCGCT GGACTTCTGG CAGAGGTACC GTGTGGAAGG CTATGGGGCT
GTGGTGCTGC CTGCCACTCC AGCGACAATG ACGTGGCCTT GGCACTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_011523262.1
CDS46..1353
Misc Feature(1)1009..>1326
Translation

Target ORF information:

RefSeq Version XM_011524960.2
Organism Homo sapiens(Human)
Definition Homo sapiens Meckel syndrome, type 1 (MKS1), transcript variant X5, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_011524960.2

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
ATGGCGGAGA CCGTCTGGAG CACTGACACC GGGGAGGCAG TGTATCGCTC CCGGGACCCC 
GTGCGCAACT TGCGCCTCCG AGTCCACCTG CAAAGAATCA CATCAAGCAA CTTTCTTCAT
TATCAGCCTG CTGCCGAGCT CGGGAAGGAC CTCATAGACT TGGCCACTTT TAGGCCTCAG
CCAACTGCCA TCTTTGGAGG TGGACACCGC CCAGAGGAAG ACGAAGAGGA GGAGATTGTG
ATTGGGTGGC AGGAGAAGCT CTTTAGCCAG TTTGAAGTAG ATCTGTACCA AAATGAAACA
GCCTGTCAGA GTCCTTTGGA TTATCAGTAC CGTCAGGAGA TCCTGAAGCT GGAGAATTCG
GGTGGCAAGA AAAACCGACG AATCTTTACC TACACTGACT CTGATAGATA CACCAATTTG
GAGGAGCACT GTCAGAGAAT GACCACTGCA GCCAGCGAGG TGCCTTCATT CTTGGTCGAG
CGAATGGCAA ATGTCAGGCG TCGCCGGCAG GACAGGCGAG GGATGGAGGG CGGCATCCTC
AAGTCACGCA TCGTCACCTG GGAGCCCTCA GAAGAGTTTG TCAGGAACAA CCACGTCATT
AACACCCCTC TTCAGACAAT GCACATCATG GCAGACCTGG GGCCCTATAA AAAGCTTGGC
TATAAGAAGT ATGAACATGT CCTGTGTACT CTGAAGGTGG ATAGCAATGG TGTGATCACA
GTAAAGCCTG ACTTCACGGG CCTCAAAGGA CCCTACAGGA TTGAGACGGA GGGGGAGAAG
CAGGAGCTGT GGAAATATAC GATCGACAAT GTTTCCCCCC ACGCACAGCC GGAGGAGGAG
GAGCGGGAAC GGCGAGTGTT CAAGGATCTT TATGGCCGGC ACAAGGAGTA TCTCAGCAGC
CTCGTAGGCA CCGACTTTGA GATGACTGTC CCAGGTGCCC TCCGGCTCTT TGTAAATGGA
GAGGTCGTTT CAGCCCAAGG CTATGAGTAT GACAATCTCT ACGTCCACTT CTTTGTAGAA
TTGCCAACTG CTCACTGGTC AAGCCCAGCA TTCCAGCAGC TCTCAGGAGT AACACAGACC
TGCACCACCA AGTCCCTGGC AATGGACAAG GTGGCTCACT TCTCCTACCC ATTCACGTTT
GAAGCCTTCT TCCTCCATGA GGATGAATCT TCTGATGCAC TCCCGGAGTG GCCTGTGCTC
TACTGTGAGG TCCTCTCGCT GGACTTCTGG CAGAGGTACC GTGTGGAAGG CTATGGGGCT
GTGGTGCTGC CTGCCACTCC AGCGACAATG ACGTGGCCTT GGCACTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu86960
Clone ID Related Accession (Same CDS sequence) XM_017024805.1
Accession Version XM_017024805.1 Latest version! Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1251bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2016-06-06
Organism Homo sapiens(Human)
Product Meckel syndrome type 1 protein isoform X8
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000017.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
ATGACCACTG CAGCCAGCGA GGTGCCTTCA TTCTTGGTCG AGCGAATGGC AAATGTCAGG 
CGTCGCCGGC AGGACAGGCG AGGGATGGAG GGCGGCATCC TCAAGTCACG CATCGTCACC
TGGGAGCCCT CAGAAGAGTT TGTCAGGAAC AACCACGTCA TTAACACCCC TCTTCAGACA
ATGCACATCA TGGCAGACCT GGGGCCCTAT AAAAAGCTTG GCTATAAGAA GTATGAACAT
GTCCTGTGTA CTCTGAAGGT GGATAGCAAT GGTGTGATCA CAGTAAAGCC TGACTTCACG
GGCCTCAAAG GACCCTACAG GATTGAGACG GAGGGGGAGA AGCAGGAGCT GTGGAAATAT
ACGATCGACA ATGTTTCCCC CCACGCACAG CCGGAGGAGG AGGAGCGGGA ACGGCGAGTG
TTCAAGGATC TTTATGGCCG GCACAAGGAG TATCTCAGCA GCCTCGTAGG CACCGACTTT
GAGATGACTG TCCCAGGTGC CCTCCGGCTC TTTGTAAATG GAGAGGTCGT TTCAGCCCAA
GGCTATGAGT ATGACAATCT CTACGTCCAC TTCTTTGTAG AATTGCCAAC TGCTCACTGG
TCAAGCCCAG CATTCCAGCA GCTCTCAGGA GTAACACAGA CCTGCACCAC CAAGTCCCTG
GCAATGGACA AGGTGGCTCA CTTCTCCTAC CCATTCACGT TTGAAGCCTT CTTCCTCCAT
GAGGATGAAT CTTCTGATGC ACTCCCGGAG TGGCCTGTGC TCTACTGTGA GGTCCTCTCG
CTGGACTTCT GGCAGAGGTA CCGTGTGGAA GGCTATGGGG CTGTGGTGCT GCCTGCCACT
CCAGGCTCAC ACACCCTGAC AGTCTCCACG TGGAGACCTG TGGAGCTTGG CACGGTGGCT
GAGCTGAGGA GGTTTTTCAT TGGCGGTTCT CTGGAACTGG AGGACCTCTC CTATGTACGG
ATACCAGGAT CCTTCAAGGG GGAACGCCTG AGCCGCTTTG GACTCCGCAC AGAGACCACA
GGCACTGTCA CCTTCCGCTT GCACTGTCTG CAGCAGTCCA GGGCCTTCAT GGAATCGAGC
TCCCTTCAGA AAAGGATGCG GAGTGTGTTG GACCGTCTGG AAGGGTTCAG CCAGCAGAGT
TCCATTCACA ATGTGCTAGA GGCCTTCCGT CGAGCCCGGC GCCGCATGCA GGAGGCCCGG
GAAAGCCTCC CGCAGGACCT AGTGAGCCCC TCTGGAACCC TGGTCTCCTA G

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_016880294.1
CDS519..1769
Misc Feature(1)1044..1565
Translation

Target ORF information:

RefSeq Version XM_017024805.1
Organism Homo sapiens(Human)
Definition Homo sapiens Meckel syndrome, type 1 (MKS1), transcript variant X8, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_017024805.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
ATGACCACTG CAGCCAGCGA GGTGCCTTCA TTCTTGGTCG AGCGAATGGC AAATGTCAGG 
CGTCGCCGGC AGGACAGGCG AGGGATGGAG GGCGGCATCC TCAAGTCACG CATCGTCACC
TGGGAGCCCT CAGAAGAGTT TGTCAGGAAC AACCACGTCA TTAACACCCC TCTTCAGACA
ATGCACATCA TGGCAGACCT GGGGCCCTAT AAAAAGCTTG GCTATAAGAA GTATGAACAT
GTCCTGTGTA CTCTGAAGGT GGATAGCAAT GGTGTGATCA CAGTAAAGCC TGACTTCACG
GGCCTCAAAG GACCCTACAG GATTGAGACG GAGGGGGAGA AGCAGGAGCT GTGGAAATAT
ACGATCGACA ATGTTTCCCC CCACGCACAG CCGGAGGAGG AGGAGCGGGA ACGGCGAGTG
TTCAAGGATC TTTATGGCCG GCACAAGGAG TATCTCAGCA GCCTCGTAGG CACCGACTTT
GAGATGACTG TCCCAGGTGC CCTCCGGCTC TTTGTAAATG GAGAGGTCGT TTCAGCCCAA
GGCTATGAGT ATGACAATCT CTACGTCCAC TTCTTTGTAG AATTGCCAAC TGCTCACTGG
TCAAGCCCAG CATTCCAGCA GCTCTCAGGA GTAACACAGA CCTGCACCAC CAAGTCCCTG
GCAATGGACA AGGTGGCTCA CTTCTCCTAC CCATTCACGT TTGAAGCCTT CTTCCTCCAT
GAGGATGAAT CTTCTGATGC ACTCCCGGAG TGGCCTGTGC TCTACTGTGA GGTCCTCTCG
CTGGACTTCT GGCAGAGGTA CCGTGTGGAA GGCTATGGGG CTGTGGTGCT GCCTGCCACT
CCAGGCTCAC ACACCCTGAC AGTCTCCACG TGGAGACCTG TGGAGCTTGG CACGGTGGCT
GAGCTGAGGA GGTTTTTCAT TGGCGGTTCT CTGGAACTGG AGGACCTCTC CTATGTACGG
ATACCAGGAT CCTTCAAGGG GGAACGCCTG AGCCGCTTTG GACTCCGCAC AGAGACCACA
GGCACTGTCA CCTTCCGCTT GCACTGTCTG CAGCAGTCCA GGGCCTTCAT GGAATCGAGC
TCCCTTCAGA AAAGGATGCG GAGTGTGTTG GACCGTCTGG AAGGGTTCAG CCAGCAGAGT
TCCATTCACA ATGTGCTAGA GGCCTTCCGT CGAGCCCGGC GCCGCATGCA GGAGGCCCGG
GAAAGCCTCC CGCAGGACCT AGTGAGCCCC TCTGGAACCC TGGTCTCCTA G

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu102306
Clone ID Related Accession (Same CDS sequence) NM_001330397.1
Accession Version NM_001330397.1 Latest version! Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1371bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2017-04-24
Organism Homo sapiens(Human)
Product Meckel syndrome type 1 protein isoform 5
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC005962.1 and BE327525.1. On Aug 29, 2016 this sequence version replaced XM_017024803.1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660805.177428.1, SRR1803614.63856.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
ATGGCGGAGA CCGTCTGGAG CACTGACACC GGGGAGGCAG TGTATCGCTC CCGGGACCCC 
GTGCGCAACT TGCGCCTCCG AGTCCACCTG CAAAGAATCA CATCAAGCAA CTTTCTTCAT
TATCAGCCTG CTGCCGAGCT CGGGAAGGAC CTCATAGACT TGGCCACTTT TAGGCCTCAG
CCAACTGCCA GTGGACACCG CCCAGAGGAA GACGAAGAGG AGGAGATTGT GATTGGGTGG
CAGGAGAAGC TCTTTAGCCA GTTTGAAGTA GATCTGTACC AAAATGAAAC AGCCTGTCAG
AGTCCTTTGG ATTATCAGTA CCGTCAGGAG ATCCTGAAGC TGGAGAATTC GGGTGGCAAG
AAAAACCGAC GAATCTTTAC CTACACTGAC TCTGATAGAT ACACCAATTT GGAGGAGCAC
TGTCAGAGAA TGACCACTGC AGCCAGCGAG GTGCCTTCAT TCTTGGTCGA GCGAATGGCA
AATGTCAGGC GTCGCCGGCA GGACAGGCGA GGGATGGAGG GCGGCATCCT CAAGTCACGC
ATCGTCACCT GGGAGCCCTC AGAAGAGTTT GTCAGGAACA ACCACGTCAT TAACACCCCT
CTTCAGACAA TGCACATCAT GGCAGACCTG GGGCCCTATA AAAAGCTTGG CTATAAGAAG
TATGAACATG TCCTGTGTAC TCTGAAGGTG GATAGCAATG GTGTGATCAC AGTAAAGCCT
GACTTCACGG GCCTCAAAGG ACCCTACAGG ATTGAGACGG AGGGGGAGAA GCAGGAGCTG
TGGAAATATA CGATCGACAA TGTTTCCCCC CACGCACAGC CGGAGGAGGA GGAGCGGGAA
CGGCGAGTGT TCAAGGATCT TTATGGCCGG CACAAGGAGT ATCTCAGCAG CCTCGTAGGC
ACCGACTTTG AGATGACTGT CCCAGGTGCC CTCCGGCTCT TTGTAAATGG AGAGGTCGTT
TCAGCCCAAG GCTATGAGTA TGACAATCTC TACGTCCACT TCTTTGTAGA ATTGCCAACT
GCTCACTGGT CAAGCCCAGC ATTCCAGCAG CTCTCAGGAG TAACACAGAC CTGCACCACC
AAGTCCCTGG CAATGGACAA GGTGGCTCAC TTCTCCTACC CATTCACGTT TGAAGCCTTC
TTCCTCCATG AGGATGAATC TTCTGATGCA CTCCCGGAGT GGCCTGTGCT CTACTGTGAG
GTCCTCTCGC TGGACTTCTG GCAGAGGTAC CGTGTGGAAG GCTATGGGGC TGTGGTGCTG
CCTGCCACTC CAGGGCCTTC ATGGAATCGA GCTCCCTTCA GAAAAGGATG CGGAGTGTGT
TGGACCGTCT GGAAGGGTTC AGCCAGCAGA GTTCCATTCA CAATGTGCTA G

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001317326.1
CDS76..1446
Exon (1)1..155
Exon (2)156..265
Exon (3)266..336
Exon (4)337..492
Exon (5)493..590
Exon (6)591..719
Exon (7)720..824
Exon (8)825..933
Exon (9)934..990
Exon (10)991..1033
Exon (11)1034..1099
Exon (12)1100..1170
Exon (13)1171..1240
Exon (14)1241..1348
Exon (15)1349..1446
Exon (16)1447..2181
Translation

Target ORF information:

RefSeq Version NM_001330397.1
Organism Homo sapiens(Human)
Definition Homo sapiens Meckel syndrome, type 1 (MKS1), transcript variant 5, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_001330397.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
ATGGCGGAGA CCGTCTGGAG CACTGACACC GGGGAGGCAG TGTATCGCTC CCGGGACCCC 
GTGCGCAACT TGCGCCTCCG AGTCCACCTG CAAAGAATCA CATCAAGCAA CTTTCTTCAT
TATCAGCCTG CTGCCGAGCT CGGGAAGGAC CTCATAGACT TGGCCACTTT TAGGCCTCAG
CCAACTGCCA GTGGACACCG CCCAGAGGAA GACGAAGAGG AGGAGATTGT GATTGGGTGG
CAGGAGAAGC TCTTTAGCCA GTTTGAAGTA GATCTGTACC AAAATGAAAC AGCCTGTCAG
AGTCCTTTGG ATTATCAGTA CCGTCAGGAG ATCCTGAAGC TGGAGAATTC GGGTGGCAAG
AAAAACCGAC GAATCTTTAC CTACACTGAC TCTGATAGAT ACACCAATTT GGAGGAGCAC
TGTCAGAGAA TGACCACTGC AGCCAGCGAG GTGCCTTCAT TCTTGGTCGA GCGAATGGCA
AATGTCAGGC GTCGCCGGCA GGACAGGCGA GGGATGGAGG GCGGCATCCT CAAGTCACGC
ATCGTCACCT GGGAGCCCTC AGAAGAGTTT GTCAGGAACA ACCACGTCAT TAACACCCCT
CTTCAGACAA TGCACATCAT GGCAGACCTG GGGCCCTATA AAAAGCTTGG CTATAAGAAG
TATGAACATG TCCTGTGTAC TCTGAAGGTG GATAGCAATG GTGTGATCAC AGTAAAGCCT
GACTTCACGG GCCTCAAAGG ACCCTACAGG ATTGAGACGG AGGGGGAGAA GCAGGAGCTG
TGGAAATATA CGATCGACAA TGTTTCCCCC CACGCACAGC CGGAGGAGGA GGAGCGGGAA
CGGCGAGTGT TCAAGGATCT TTATGGCCGG CACAAGGAGT ATCTCAGCAG CCTCGTAGGC
ACCGACTTTG AGATGACTGT CCCAGGTGCC CTCCGGCTCT TTGTAAATGG AGAGGTCGTT
TCAGCCCAAG GCTATGAGTA TGACAATCTC TACGTCCACT TCTTTGTAGA ATTGCCAACT
GCTCACTGGT CAAGCCCAGC ATTCCAGCAG CTCTCAGGAG TAACACAGAC CTGCACCACC
AAGTCCCTGG CAATGGACAA GGTGGCTCAC TTCTCCTACC CATTCACGTT TGAAGCCTTC
TTCCTCCATG AGGATGAATC TTCTGATGCA CTCCCGGAGT GGCCTGTGCT CTACTGTGAG
GTCCTCTCGC TGGACTTCTG GCAGAGGTAC CGTGTGGAAG GCTATGGGGC TGTGGTGCTG
CCTGCCACTC CAGGGCCTTC ATGGAATCGA GCTCCCTTCA GAAAAGGATG CGGAGTGTGT
TGGACCGTCT GGAAGGGTTC AGCCAGCAGA GTTCCATTCA CAATGTGCTA G

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu86959
Clone ID Related Accession (Same CDS sequence) XM_017024804.1
Accession Version XM_017024804.1 Latest version! Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1299bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2016-06-06
Organism Homo sapiens(Human)
Product Meckel syndrome type 1 protein isoform X6
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000017.11) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
ATGGCGGAGA CCGTCTGGAG CACTGACACC GGGGAGGCAG TGTATCGCTC CCGGGACCCC 
GTGCGCAACT TGCGCCTCCG AGTCCACCTG CAAAGAATCA CATCAAGCAA CTTTCTTCAT
TATCAGCCTG CTGCCGAGCT CGGGAAGGAC CTCATAGACT TGGCCACTTT TAGGCCTCAG
CCAACTGCCA GTGGACACCG CCCAGAGGAA GACGAAGAGG AGGAGATTGT GATTGGGTGG
CAGGAGAAGC TCTTTAGCCA GTTTGAAGTA GATCTGTACC AAAATGAAAC AGCCTGTCAG
AGTCCTTTGG ATTATCAGTA CCGTCAGGAG ATCCTGAAGC TGGAGAATTC GGGTGGCAAG
AAAAACCGAC GAATCTTTAC CTACACTGAC TCTGATAGAT ACACCAATTT GGAGGAGCAC
TGTCAGAGAA TGACCACTGC AGCCAGCGAG GTGCCTTCAT TCTTGGTCGA GCGAATGGCA
AATGTCAGGC GTCGCCGGCA GGACAGGCGA GGGATGGAGG GCGGCATCCT CAAGTCACGC
ATCGTCACCT GGGAGCCCTC AGAAGAGTTT GTCAGGAACA ACCACGTCAT TAACACCCCT
CTTCAGACAA TGCACATCAT GGCAGACCTG GGGCCCTATA AAAAGCTTGG CTATAAGAAG
TATGAACATG TCCTGTGTAC TCTGAAGGTG GATAGCAATG GTGTGATCAC AGTAAAGCCT
GACTTCACGG GCCTCAAAGG ACCCTACAGG ATTGAGACGG AGGGGGAGAA GCAGGAGCTG
TGGAAATATA CGATCGACAA TGTTTCCCCC CACGCACAGC CGGAGGAGGA GGAGCGGGAA
CGGCGAGTGT TCAAGGATCT TTATGGCCGG CACAAGGAGT ATCTCAGCAG CCTCGTAGGC
ACCGACTTTG AGATGACTGT CCCAGGTGCC CTCCGGCTCT TTGTAAATGG AGAGGTCGTT
TCAGCCCAAG GCTATGAGTA TGACAATCTC TACGTCCACT TCTTTGTAGA ATTGCCAACT
GCTCACTGGT CAAGCCCAGC ATTCCAGCAG CTCTCAGGAG TAACACAGAC CTGCACCACC
AAGTCCCTGG CAATGGACAA GGTGGCTCAC TTCTCCTACC CATTCACGTT TGAAGCCTTC
TTCCTCCATG AGGATGAATC TTCTGATGCA CTCCCGGAGT GGCCTGTGCT CTACTGTGAG
GTCCTCTCGC TGGACTTCTG GCAGAGGTAC CGTGTGGAAG GCTATGGGGC TGTGGTGCTG
CCTGCCACTC CAGCGACAAT GACGTGGCCT TGGCACTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_016880293.1
CDS24..1322
Translation

Target ORF information:

RefSeq Version XM_017024804.1
Organism Homo sapiens(Human)
Definition Homo sapiens Meckel syndrome, type 1 (MKS1), transcript variant X6, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_017024804.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
ATGGCGGAGA CCGTCTGGAG CACTGACACC GGGGAGGCAG TGTATCGCTC CCGGGACCCC 
GTGCGCAACT TGCGCCTCCG AGTCCACCTG CAAAGAATCA CATCAAGCAA CTTTCTTCAT
TATCAGCCTG CTGCCGAGCT CGGGAAGGAC CTCATAGACT TGGCCACTTT TAGGCCTCAG
CCAACTGCCA GTGGACACCG CCCAGAGGAA GACGAAGAGG AGGAGATTGT GATTGGGTGG
CAGGAGAAGC TCTTTAGCCA GTTTGAAGTA GATCTGTACC AAAATGAAAC AGCCTGTCAG
AGTCCTTTGG ATTATCAGTA CCGTCAGGAG ATCCTGAAGC TGGAGAATTC GGGTGGCAAG
AAAAACCGAC GAATCTTTAC CTACACTGAC TCTGATAGAT ACACCAATTT GGAGGAGCAC
TGTCAGAGAA TGACCACTGC AGCCAGCGAG GTGCCTTCAT TCTTGGTCGA GCGAATGGCA
AATGTCAGGC GTCGCCGGCA GGACAGGCGA GGGATGGAGG GCGGCATCCT CAAGTCACGC
ATCGTCACCT GGGAGCCCTC AGAAGAGTTT GTCAGGAACA ACCACGTCAT TAACACCCCT
CTTCAGACAA TGCACATCAT GGCAGACCTG GGGCCCTATA AAAAGCTTGG CTATAAGAAG
TATGAACATG TCCTGTGTAC TCTGAAGGTG GATAGCAATG GTGTGATCAC AGTAAAGCCT
GACTTCACGG GCCTCAAAGG ACCCTACAGG ATTGAGACGG AGGGGGAGAA GCAGGAGCTG
TGGAAATATA CGATCGACAA TGTTTCCCCC CACGCACAGC CGGAGGAGGA GGAGCGGGAA
CGGCGAGTGT TCAAGGATCT TTATGGCCGG CACAAGGAGT ATCTCAGCAG CCTCGTAGGC
ACCGACTTTG AGATGACTGT CCCAGGTGCC CTCCGGCTCT TTGTAAATGG AGAGGTCGTT
TCAGCCCAAG GCTATGAGTA TGACAATCTC TACGTCCACT TCTTTGTAGA ATTGCCAACT
GCTCACTGGT CAAGCCCAGC ATTCCAGCAG CTCTCAGGAG TAACACAGAC CTGCACCACC
AAGTCCCTGG CAATGGACAA GGTGGCTCAC TTCTCCTACC CATTCACGTT TGAAGCCTTC
TTCCTCCATG AGGATGAATC TTCTGATGCA CTCCCGGAGT GGCCTGTGCT CTACTGTGAG
GTCCTCTCGC TGGACTTCTG GCAGAGGTAC CGTGTGGAAG GCTATGGGGC TGTGGTGCTG
CCTGCCACTC CAGCGACAAT GACGTGGCCT TGGCACTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

book

MKS1 regulates ciliary INPP5E levels in Joubert syndrome.
Journal of medical genetics53(1)62-72(2016 Jan)
Slaats GG,Isabella CR,Kroes HY,Dempsey JC,Gremmels H,Monroe GR,Phelps IG,Duran KJ,Adkins J,Kumar SA,Knutzen DM,Knoers NV,Mendelsohn NJ,Neubauer D,Mastroyianni SD,Vogt J,Worgan L,Karp N,Bowdin S,Glass IA,Parisi MA,Otto EA,Johnson CA,Hildebrandt F,van Haaften G,Giles RH,Doherty D


book

Mutations in B9D1 and MKS1 cause mild Joubert syndrome: expanding the genetic overlap with the lethal ciliopathy Meckel syndrome.
Orphanet journal of rare diseases972(2014 May)
Romani M,Micalizzi A,Kraoua I,Dotti MT,Cavallin M,Sztriha L,Ruta R,Mancini F,Mazza T,Castellana S,Hanene B,Carluccio MA,Darra F,Máté A,Zimmermann A,Gouider-Khouja N,Valente EM


book

Mutation analysis of 18 nephronophthisis associated ciliopathy disease genes using a DNA pooling and next generation sequencing strategy.
Journal of medical genetics48(2)105-16(2011 Feb)
Otto EA,Ramaswami G,Janssen S,Chaki M,Allen SJ,Zhou W,Airik R,Hurd TW,Ghosh AK,Wolf MT,Hoppe B,Neuhaus TJ,Bockenhauer D,Milford DV,Soliman NA,Antignac C,Saunier S,Johnson CA,Hildebrandt F,


 
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