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NOP10 cDNA ORF clone, Homo sapiens (human)

Gene Symbol NOP10
Entrez Gene ID 55505
Full Name NOP10 ribonucleoprotein
Synonyms DKCB1, NOLA3, NOP10P
Gene Type protein-coding
Organism Homo sapiens (human)
Genome

15

15q14-q15

Summary This gene is a member of the H/ACA snoRNPs (small nucleolar ribonucleoproteins) gene family. snoRNPs are involved in various aspects of rRNA processing and modification and have been classified into two families: C/D and H/ACA. The H/ACA snoRNPs also include the DKC1, NOLA1 and NOLA2 proteins. These four H/ACA snoRNP proteins localize to the dense fibrillar components of nucleoli and to coiled (Cajal) bodies in the nucleus. Both 18S rRNA production and rRNA pseudouridylation are impaired if any one of the four proteins is depleted. The four H/ACA snoRNP proteins are also components of the telomerase complex. This gene encodes a protein related to Saccharomyces cerevisiae Nop10p. [provided by RefSeq, Jul 2008]. lac of sum
Disorder MIM:

606471

Disorder Html: Dyskeratosis congenita, autosomal recessive, 224230 (3)

mRNA and Protein(s)

mRNA Protein Name
NM_018648 NP_061118 H/ACA ribonucleoprotein complex subunit 3



Homo sapiens (human) NOP10 NP_061118.1
Pan troglodytes (chimpanzee) NOP10 XP_001165824.1
Canis lupus familiaris (dog) NOP10 XP_849017.2
Bos taurus (cattle) NOP10 NP_001107993.1
Mus musculus (house mouse) Nop10 NP_079679.1
Rattus norvegicus (Norway rat) Nop10 NP_001119572.1
Danio rerio (zebrafish) nop10 NP_001003868.1
Drosophila melanogaster (fruit fly) CG7637 NP_610610.1
Xenopus (Silurana) tropicalis (western clawed frog) nop10 NP_001016163.1


GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following NOP10 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the NOP10 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID RefSeq Accession Definition **Vector *Turnaround time Price Select
OHu19474
NM_018648 Homo sapiens NOP10 ribonucleoprotein (NOP10), mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
$49.50-$69.30
$99.00
Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone 30% OFF
2-4 Clone 40% OFF
5 or more Clone 50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.


CloneID OHu19474
Clone ID Related Accession (Same CDS sequence) NM_018648
Accession Version NM_018648.3 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 195bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product H/ACA ribonucleoprotein complex subunit 3
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BQ218536.1 and BM554889.1. This sequence is a reference standard in the RefSeqGene project. On Oct 18, 2005 this sequence version replaced gi:15011920. ##Evidence-Data-START## Transcript exon combination :: BU955288.1, AB043104.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968189, SAMEA1968540 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
ATGTTTCTCC AGTATTACCT CAACGAGCAG GGAGATCGAG TCTATACGCT GAAGAAATTT 
GACCCGATGG GACAACAGAC CTGCTCAGCC CATCCTGCTC GGTTCTCCCC AGATGACAAA
TACTCTCGAC ACCGAATCAC CATCAAGAAA CGCTTCAAGG TGCTCATGAC CCAGCAACCG
CGCCCTGTCC TCTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_061118.1
CDS89..283
Misc Feature(1)80..82(+)
Misc Feature(2)95..247(+)
Misc Feature(3)194..196(+)
Exon (1)1..142
Gene:NOP10
Gene Synonym:
Exon (2)143..535
Gene:NOP10
Gene Synonym:
Translation

Target ORF information:

RefSeq Version NM_018648
Organism Homo sapiens (human)
Definition Homo sapiens NOP10 ribonucleoprotein (NOP10), mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_018648

ORF Insert Sequence:

1
61
121
181
ATGTTTCTCC AGTATTACCT CAACGAGCAG GGAGATCGAG TCTATACGCT GAAGAAATTT 
GACCCGATGG GACAACAGAC CTGCTCAGCC CATCCTGCTC GGTTCTCCCC AGATGACAAA
TACTCTCGAC ACCGAATCAC CATCAAGAAA CGCTTCAAGG TGCTCATGAC CCAGCAACCG
CGCCCTGTCC TCTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

book

Effects of dyskeratosis congenita mutations in dyskerin, NHP2 and NOP10 on assembly of H/ACA pre-RNPs
Hum. Mol. Genet. 19 (5), 825-836 (2010)
Trahan C, Martel C and Dragon F.


book

Genetic heterogeneity in autosomal recessive dyskeratosis congenita with one subtype due to mutations in the telomerase-associated protein NOP10
Hum. Mol. Genet. 16 (13), 1619-1629 (2007)
Walne AJ, Vulliamy T, Marrone A, Beswick R, Kirwan M, Masunari Y, Al-Qurashi FH, Aljurf M and Dokal I.


book

hNaf1 is required for accumulation of human box H/ACA snoRNPs, scaRNPs, and telomerase
RNA 12 (5), 832-840 (2006)
Hoareau-Aveilla C, Bonoli M, Caizergues-Ferrer M and Henry Y.


book

Mutations in TERT, the gene for telomerase reverse transcriptase, in aplastic anemia
N. Engl. J. Med. 352 (14), 1413-1424 (2005)
Yamaguchi H, Calado RT, Ly H, Kajigaya S, Baerlocher GM, Chanock SJ, Lansdorp PM and Young NS.


book

Nucleolar proteome dynamics
Nature 433 (7021), 77-83 (2005)
Andersen JS, Lam YW, Leung AK, Ong SE, Lyon CE, Lamond AI and Mann M.


book

Architecture and assembly of mammalian H/ACA small nucleolar and telomerase ribonucleoproteins
EMBO J. 23 (8), 1857-1867 (2004)
Wang C and Meier UT.


book

Human H/ACA small nucleolar RNPs and telomerase share evolutionarily conserved proteins NHP2 and NOP10
Mol. Cell. Biol. 20 (23), 9028-9040 (2000)
Pogacic V, Dragon F and Filipowicz W.


book

Nhp2p and Nop10p are essential for the function of H/ACA snoRNPs
EMBO J. 17 (23), 7078-7090 (1998)
Henras A, Henry Y, Bousquet-Antonelli C, Noaillac-Depeyre J, Gelugne JP and Caizergues-Ferrer M.


book

Dyskeratosis Congenita
(in) Pagon RA, Adam MP, Ardinger HH, Bird TD, Dolan CR, Fong CT, Smith RJH and Stephens K (Eds.); GENEREVIEWS(R); (1993)
Savage,S.A.


 
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