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NOP10 cDNA ORF clone, Homo sapiens(Human)

Gene Symbol NOP10
Entrez Gene ID 55505
Full Name NOP10 ribonucleoprotein
Synonyms DKCB1,NOLA3,NOP10P
General protein information
Preferred Names

NOP10 ribonucleoprotein

Names

H/ACA ribonucleoprotein complex subunit 3
NOP10 ribonucleoprotein homolog
homolog of yeast Nop10p
nucleolar protein 10
nucleolar protein family A member 3
nucleolar protein family A, member 3 (H/ACA small nucleolar RNPs)
snoRNP protein NOP10

Gene Type protein-coding
Organism Homo sapiens(Human)
Genome

15

15q14

Summary This gene is a member of the H/ACA snoRNPs (small nucleolar ribonucleoproteins) gene family. snoRNPs are involved in various aspects of rRNA processing and modification and have been classified into two families: C/D and H/ACA. The H/ACA snoRNPs also include the DKC1, NOLA1 and NOLA2 proteins. These four H/ACA snoRNP proteins localize to the dense fibrillar components of nucleoli and to coiled (Cajal) bodies in the nucleus. Both 18S rRNA production and rRNA pseudouridylation are impaired if any one of the four proteins is depleted. The four H/ACA snoRNP proteins are also components of the telomerase complex. This gene encodes a protein related to Saccharomyces cerevisiae Nop10p. [provided by RefSeq, Jul 2008].
Disorder MIM:

606471

Disorder Html: Dyskeratosis congenita, autosomal recessive, 224230 (3)

mRNA and Protein(s)

mRNA Protein Name
NM_018648.3 NP_061118.1 H/ACA ribonucleoprotein complex subunit 3



Mus musculus (house mouse) Nop10 NP_079679.1
Anopheles gambiae (African malaria mosquito) AgaP_AGAP005149 XP_314037.2
Pan troglodytes (chimpanzee) NOP10 XP_001165824.1
Canis lupus familiaris (dog) NOP10 XP_849017.2
Bos taurus (cattle) NOP10 NP_001107993.1
Homo sapiens (human) NOP10 NP_061118.1
Danio rerio (zebrafish) nop10 NP_001003868.1
Drosophila melanogaster (fruit fly) CG7637 NP_610610.1
Rattus norvegicus (Norway rat) Nop10 NP_001119572.1
Xenopus tropicalis (tropical clawed frog) nop10 NP_001016163.1


Related articles in PubMed

Widespread macromolecular interaction perturbations in human genetic disorders.
Sahni N, Yi S, Taipale M, Fuxman Bass JI, Coulombe-Huntington J, Yang F, Peng J, Weile J, Karras GI, Wang Y, Kovács IA, Kamburov A, Krykbaeva I, Lam MH, Tucker G, Khurana V, Sharma A, Liu YY, Yachie N, Zhong Q, Shen Y, Palagi A, San-Miguel A, Fan C, Balcha D, Dricot A, Jordan DM, Walsh JM, Shah AA, Yang X, Stoyanova AK, Leighton A, Calderwood MA, Jacob Y, Cusick ME, Salehi-Ashtiani K, Whitesell LJ, Sunyaev S, Berger B, Barabási AL, Charloteaux B, Hill DE, Hao T, Roth FP, Xia Y, Walhout AJM, Lindquist S, Vidal M
Cell161(3)647-660(2015 Apr)

Proteostatic control of telomerase function through TRiC-mediated folding of TCAB1.
Freund A, Zhong FL, Venteicher AS, Meng Z, Veenstra TD, Frydman J, Artandi SE
Cell159(6)1389-403(2014 Dec)

Detection of mutations in TERT, the genes for telomerase reverse transcriptase, in Indian patients of aplastic anaemia: a pilot study.
Mehta S, Krishnamohan M, Gulati S, Sharmat N, Vashishtha P, Singh I
The Journal of the Association of Physicians of India62(10)13-7(2014 Oct)

HOT1 is a mammalian direct telomere repeat-binding protein contributing to telomerase recruitment.
Kappei D, Butter F, Benda C, Scheibe M, Draškovič I, Stevense M, Novo CL, Basquin C, Araki M, Araki K, Krastev DB, Kittler R, Jessberger R, Londoño-Vallejo JA, Mann M, Buchholz F
The EMBO journal32(12)1681-701(2013 Jun)

An enhanced H/ACA RNP assembly mechanism for human telomerase RNA.
Egan ED, Collins K
Molecular and cellular biology32(13)2428-39(2012 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Indian aplastic anemia patients did not have NOP10 mutations.
Title: Detection of mutations in TERT, the genes for telomerase reverse transcriptase, in Indian patients of aplastic anaemia: a pilot study.

Effects of dyskeratosis congenita mutations in NOP10 on assembly of H/ACA pre-RNPs
Title: Effects of dyskeratosis congenita mutations in dyskerin, NHP2 and NOP10 on assembly of H/ACA pre-RNPs.

NOP10 has a role in the telomerase complex and telomere maintenance, and in autosomal recessive dyskeratosis congenita
Title: Genetic heterogeneity in autosomal recessive dyskeratosis congenita with one subtype due to mutations in the telomerase-associated protein NOP10.

Observational study of gene-disease association. (HuGE Navigator)
Title: Mutations in TERT, the gene for telomerase reverse transcriptase, in aplastic anemia.

The following NOP10 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the NOP10 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

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***CloneID RefSeq Accession Definition **Vector *Turnaround time Price (USD) Select
OHu19474
NM_018648.3
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Homo sapiens NOP10 ribonucleoprotein (NOP10), mRNA. pcDNA3.1+/C-(K)DYK or customized vector
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.


CloneID OHu19474
Clone ID Related Accession (Same CDS sequence) NM_018648.3
Accession Version NM_018648.3 Latest version! Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 195bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2017-04-27
Organism Homo sapiens(Human)
Product H/ACA ribonucleoprotein complex subunit 3
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BQ218536.1 and BM554889.1. This sequence is a reference standard in the RefSeqGene project. On Oct 18, 2005 this sequence version replaced NM_018648.2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BU955288.1, AB043104.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968189, SAMEA1968540 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
ATGTTTCTCC AGTATTACCT CAACGAGCAG GGAGATCGAG TCTATACGCT GAAGAAATTT 
GACCCGATGG GACAACAGAC CTGCTCAGCC CATCCTGCTC GGTTCTCCCC AGATGACAAA
TACTCTCGAC ACCGAATCAC CATCAAGAAA CGCTTCAAGG TGCTCATGAC CCAGCAACCG
CGCCCTGTCC TCTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_061118.1
CDS89..283
Misc Feature(1)80..82
Misc Feature(2)104..244
Misc Feature(3)194..196
Exon (1)1..142
Exon (2)143..535
Translation

Target ORF information:

RefSeq Version NM_018648.3
Organism Homo sapiens(Human)
Definition Homo sapiens NOP10 ribonucleoprotein (NOP10), mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_018648.3

ORF Insert Sequence:

1
61
121
181
ATGTTTCTCC AGTATTACCT CAACGAGCAG GGAGATCGAG TCTATACGCT GAAGAAATTT 
GACCCGATGG GACAACAGAC CTGCTCAGCC CATCCTGCTC GGTTCTCCCC AGATGACAAA
TACTCTCGAC ACCGAATCAC CATCAAGAAA CGCTTCAAGG TGCTCATGAC CCAGCAACCG
CGCCCTGTCC TCTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

book

Widespread macromolecular interaction perturbations in human genetic disorders.
Cell161(3)647-660(2015 Apr)
Sahni N,Yi S,Taipale M,Fuxman Bass JI,Coulombe-Huntington J,Yang F,Peng J,Weile J,Karras GI,Wang Y,Kovács IA,Kamburov A,Krykbaeva I,Lam MH,Tucker G,Khurana V,Sharma A,Liu YY,Yachie N,Zhong Q,Shen Y,Palagi A,San-Miguel A,Fan C,Balcha D,Dricot A,Jordan DM,Walsh JM,Shah AA,Yang X,Stoyanova AK,Leighton A,Calderwood MA,Jacob Y,Cusick ME,Salehi-Ashtiani K,Whitesell LJ,Sunyaev S,Berger B,Barabási AL,Charloteaux B,Hill DE,Hao T,Roth FP,Xia Y,Walhout AJM,Lindquist S,Vidal M


book

Proteostatic control of telomerase function through TRiC-mediated folding of TCAB1.
Cell159(6)1389-403(2014 Dec)
Freund A,Zhong FL,Venteicher AS,Meng Z,Veenstra TD,Frydman J,Artandi SE


book

Detection of mutations in TERT, the genes for telomerase reverse transcriptase, in Indian patients of aplastic anaemia: a pilot study.
The Journal of the Association of Physicians of India62(10)13-7(2014 Oct)
Mehta S,Krishnamohan M,Gulati S,Sharmat N,Vashishtha P,Singh I


book

HOT1 is a mammalian direct telomere repeat-binding protein contributing to telomerase recruitment.
The EMBO journal32(12)1681-701(2013 Jun)
Kappei D,Butter F,Benda C,Scheibe M,Draškovič I,Stevense M,Novo CL,Basquin C,Araki M,Araki K,Krastev DB,Kittler R,Jessberger R,Londoño-Vallejo JA,Mann M,Buchholz F


book

An enhanced H/ACA RNP assembly mechanism for human telomerase RNA.
Molecular and cellular biology32(13)2428-39(2012 Jul)
Egan ED,Collins K


 
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