PRKCQ cDNA ORF clone, Homo sapiens(human)
Gene Symbol | PRKCQ |
Entrez Gene ID | 5588 |
Full Name | protein kinase C theta |
Synonyms | PRKCT,nPKC-theta |
General protein information |
|
Gene Type | protein-coding |
Organism | Homo sapiens(human) |
Genome | |
Summary | Protein kinase C (PKC) is a family of serine- and threonine-specific protein kinases that can be activated by calcium and the second messenger diacylglycerol. PKC family members phosphorylate a wide variety of protein targets and are known to be involved in diverse cellular signaling pathways. PKC family members also serve as major receptors for phorbol esters, a class of tumor promoters. Each member of the PKC family has a specific expression profile and is believed to play a distinct role. The protein encoded by this gene is one of the PKC family members. It is a calcium-independent and phospholipid-dependent protein kinase. This kinase is important for T-cell activation. It is required for the activation of the transcription factors NF-kappaB and AP-1, and may link the T cell receptor (TCR) signaling complex to the activation of the transcription factors. [provided by RefSeq, Jul 2008]. |
Disorder MIM: |
mRNA and Protein(s)
mRNA | Protein | Name |
---|---|---|
XM_006717465.3 | XP_006717528.1 | protein kinase C theta type isoform X2 |
XM_024448076.1 | XP_024303844.1 | protein kinase C theta type isoform X2 |
NM_006257.5 | NP_006248.1 | protein kinase C theta type isoform 1 |
NM_001323267.1 | NP_001310196.1 | protein kinase C theta type isoform 5 |
NM_001323266.2 | NP_001310195.1 | protein kinase C theta type isoform 4 |
XM_006717465.2 | XP_006717528.1 | protein kinase C theta type isoform X1 |
XM_005252497.4 | XP_005252554.1 | protein kinase C theta type isoform X3 |
XM_024448077.1 | XP_024303845.1 | protein kinase C theta type isoform X4 |
NM_001242413.2 | NP_001229342.1 | protein kinase C theta type isoform 2 |
XM_011519547.1 | XP_011517849.1 | protein kinase C theta type isoform X1 |
XM_017016410.1 | XP_016871899.1 | protein kinase C theta type isoform X2 |
NM_006257.4 | NP_006248.1 | protein kinase C theta type isoform 1 |
NM_001323266.1 | NP_001310195.1 | protein kinase C theta type isoform 4 |
NM_001282644.2 | NP_001269573.1 | protein kinase C theta type isoform 3 |
NM_001282645.1 | NP_001269574.1 | protein kinase C theta type isoform 4 |
NM_001282644.1 | NP_001269573.1 | protein kinase C theta type isoform 3 |
XM_005252496.4 | XP_005252553.1 | protein kinase C theta type isoform X1 |
NM_001323265.1 | NP_001310194.1 | protein kinase C theta type isoform 1 |
XM_017016411.1 | XP_016871900.1 | protein kinase C theta type isoform X4 |

Homo sapiens (human) | PRKCQ | NP_006248.1 |
Pan troglodytes (chimpanzee) | PRKCQ | XP_001148074.1 |
Canis lupus familiaris (dog) | PRKCQ | XP_005617224.1 |
Gallus gallus (chicken) | PRKCQ | XP_001235980.2 |
PRKCQ | XP_001118689.2 | |
Rattus norvegicus (Norway rat) | Prkcq | NP_001263650.1 |
Danio rerio (zebrafish) | prkcq | NP_001082839.2 |
Bos taurus (cattle) | PRKCQ | NP_001179006.1 |
Mus musculus (house mouse) | Prkcq | NP_032885.1 |

Related articles in PubMed
Structural determinants of phorbol ester binding activity of the C1a and C1b domains of protein kinase C theta.
Czikora A, Pany S, You Y, Saini AS, Lewin NE, Mitchell GA, Abramovitz A, Kedei N, Blumberg PM, Das J
Biochimica et biophysica acta(2018 Jan)
Protein kinase C? controls type 2 innate lymphoid cell and T
Madouri F, Chenuet P, Beuraud C, Fauconnier L, Marchiol T, Rouxel N, Ledru A, Gallerand M, Lombardi V, Mascarell L, Marquant Q, Apetoh L, Erard F, Le Bert M, Trovero F, Quesniaux VFJ, Ryffel B, Togbe D
The Journal of allergy and clinical immunology139(5)1650-1666(2017 May)
eNOS S-nitrosylates ?-actin on Cys374 and regulates PKC-? at the immune synapse by impairing actin binding to profilin-1.
Garc?a-Ortiz A, Mart?n-Cofreces NB, Ibiza S, Ortega ?, Izquierdo-?lvarez A, Trullo A, Victor VM, Calvo E, Sot B, Mart?nez-Ruiz A, V?zquez J, S?nchez-Madrid F, Serrador JM
PLoS biology15(4)e2000653(2017 Apr)
PKC?-induced phosphorylations control the ability of Fra-1 to stimulate gene expression and cancer cell migration.
Belguise K, Cherradi S, Sarr A, Boissi?re F, Boulle N, Simony-Lafontaine J, Choesmel-Cadamuro V, Wang X, Chalbos D
Cancer letters38597-107(2017 01)
GeneRIFs: Gene References Into Functions What's a GeneRIF?
PKC-theta; is a critical factor for type 2 innate lymphoid cells activation that contributes to TH2 cell differentiation, which is associated with IRF4 and NFAT1 expression in allergic lung inflammation.
Title: Protein kinase C? controls type 2 innate lymphoid cell and T
Data suggest that, in T-lymphocytes, nitric oxide generated by eNOS S-nitrosylates Cys374 on ACTB and thus regulates activation/recruitment of PRKCQ at immune synapse; S-nitrosylation of beta-actin impairs actin binding to PFN1 and regulates protein transport in lamellipodia. (eNOS = nitric oxide synthase 3; ACTB = beta-actin; PRKCQ = protein kinase C-theta; PFN1 = profilin-1)
Title: eNOS S-nitrosylates ?-actin on Cys374 and regulates PKC-? at the immune synapse by impairing actin binding to profilin-1.
PKCtheta;-induced phosphorylations control the ability of Fra-1 to stimulate gene expression and breast cancer cell migration.
Title: PKC?-induced phosphorylations control the ability of Fra-1 to stimulate gene expression and cancer cell migration.
PCR and transcriptome analysis suggest that the genes CD3D and PKRCQ together can be used as a model for differentiating between B-cell and T-cell acute lymphoblastic leukemia.
Title: CD3D and PRKCQ work together to discriminate between B-cell and T-cell acute lymphoblastic leukemia.
Enhanced PRKCQ/PKCtheta; expression can promote growth-factor-independent growth, anoikis resistance, and migration. PRKCQ critically regulates growth and survival of a subset of TNBC.
Title: PRKCQ promotes oncogenic growth and anoikis resistance of a subset of triple-negative breast cancer cells.
The following PRKCQ gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the PRKCQ cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.
CloneID | OHu20978![]() |
|
Clone ID Related Accession (Same CDS sequence) | XM_006717465.3 , XM_024448076.1 , NM_006257.5 , XM_006717465.2 , XM_011519547.1 , XM_017016410.1 , NM_006257.4 , NM_001323265.1 | |
Accession Version | NM_006257.5 Latest version! | Documents for ORF clone product in default vector |
Sequence Information | ORF Nucleotide Sequence (Length: 2121bp) Protein sequence SNP |
|
Vector | pcDNA3.1+/C-(K)DYK or customized vector | ![]() |
Clone information | Clone Map | ![]() |
Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
ORF Insert Method | CloneEZ™ Seamless cloning technology | |
Insert Structure | linear | |
Update Date | 2019-12-31 | |
Organism | Homo sapiens(human) | |
Product | protein kinase C theta type isoform 1 | |
Comment | Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AK308099.1, AK312411.1, AK293935.1, BX647657.1, AA234440.1 and R40214.1. On May 17, 2019 this sequence version replaced NM_006257.4. Transcript Variant: This variant (1) encodes the longest isoform (1). Variants 1 and 5 both encode the same isoform (1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660805.211514.1, SRR1660807.38215.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000263125.10/ ENSP00000263125.5 RefSeq Select criteria :: based on conservation, expression ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end. |
1 | ATGTCGCCAT TTCTTCGGAT TGGCTTGTCC AACTTTGACT GCGGGTCCTG CCAGTCTTGT |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
RefSeq | NP_006248.1 |
CDS | 76..2196 |
Translation |

Target ORF information:
Target ORF information:
|
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ORF Insert Sequence:
1 | ATGTCGCCAT TTCTTCGGAT TGGCTTGTCC AACTTTGACT GCGGGTCCTG CCAGTCTTGT |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
CloneID | OHu20978![]() |
|
Clone ID Related Accession (Same CDS sequence) | XM_006717465.3 , XM_024448076.1 , NM_006257.5 , XM_006717465.2 , XM_011519547.1 , XM_017016410.1 , NM_006257.4 , NM_001323265.1 | |
Accession Version | NM_006257.4 | Documents for ORF clone product in default vector |
Sequence Information | ORF Nucleotide Sequence (Length: 2121bp) Protein sequence SNP |
|
Vector | pcDNA3.1+/C-(K)DYK or customized vector | ![]() |
Clone information | Clone Map | ![]() |
Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
ORF Insert Method | CloneEZ™ Seamless cloning technology | |
Insert Structure | linear | |
Update Date | 2019-02-23 | |
Organism | Homo sapiens(human) | |
Product | protein kinase C theta type isoform 1 | |
Comment | Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from L01087.1, AK308099.1, AK312411.1, AK293935.1, BX647657.1, AA234440.1 and R40214.1. On Sep 17, 2013 this sequence version replaced NM_006257.3. Transcript Variant: This variant (1) encodes the longest isoform (1). Variants 1 and 5 both encode the same isoform (1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803617.29613.1, SRR1803616.101014.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end. |
1 | ATGTCGCCAT TTCTTCGGAT TGGCTTGTCC AACTTTGACT GCGGGTCCTG CCAGTCTTGT |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
RefSeq | NP_006248.1 |
CDS | 101..2221 |
Translation |

Target ORF information:
Target ORF information:
|
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ORF Insert Sequence:
1 | ATGTCGCCAT TTCTTCGGAT TGGCTTGTCC AACTTTGACT GCGGGTCCTG CCAGTCTTGT |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
CloneID | OHu20978![]() |
|
Clone ID Related Accession (Same CDS sequence) | XM_006717465.3 , XM_024448076.1 , NM_006257.5 , XM_006717465.2 , XM_011519547.1 , XM_017016410.1 , NM_006257.4 , NM_001323265.1 | |
Accession Version | NM_001323265.1 Latest version! | Documents for ORF clone product in default vector |
Sequence Information | ORF Nucleotide Sequence (Length: 2121bp) Protein sequence SNP |
|
Vector | pcDNA3.1+/C-(K)DYK or customized vector | ![]() |
Clone information | Clone Map | ![]() |
Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
ORF Insert Method | CloneEZ™ Seamless cloning technology | |
Insert Structure | linear | |
Update Date | 2019-12-28 | |
Organism | Homo sapiens(human) | |
Product | protein kinase C theta type isoform 1 | |
Comment | Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AL158043.14 and AL137145.14. On Apr 19, 2016 this sequence version replaced XM_011519547.1. Transcript Variant: This variant (5) and variant 1 both encode isoform 1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660807.264210.1, SRR1803612.12077.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end. |
1 | ATGTCGCCAT TTCTTCGGAT TGGCTTGTCC AACTTTGACT GCGGGTCCTG CCAGTCTTGT |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
RefSeq | NP_001310194.1 |
CDS | 156..2276 |
Translation |

Target ORF information:
Target ORF information:
|
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ORF Insert Sequence:
1 | ATGTCGCCAT TTCTTCGGAT TGGCTTGTCC AACTTTGACT GCGGGTCCTG CCAGTCTTGT |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
CloneID | OHu20978![]() |
|
Clone ID Related Accession (Same CDS sequence) | XM_006717465.3 , XM_024448076.1 , NM_006257.5 , XM_006717465.2 , XM_011519547.1 , XM_017016410.1 , NM_006257.4 , NM_001323265.1 | |
Accession Version | XM_006717465.3 Latest version! | Documents for ORF clone product in default vector |
Sequence Information | ORF Nucleotide Sequence (Length: 2121bp) Protein sequence SNP |
|
Vector | pcDNA3.1+/C-(K)DYK or customized vector | ![]() |
Clone information | Clone Map | ![]() |
Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
ORF Insert Method | CloneEZ™ Seamless cloning technology | |
Insert Structure | linear | |
Update Date | 2016-06-05 | |
Organism | Homo sapiens(human) | |
Product | protein kinase C theta type isoform X2 | |
Comment | Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000010.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_006717465.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## |
1 | ATGTCGCCAT TTCTTCGGAT TGGCTTGTCC AACTTTGACT GCGGGTCCTG CCAGTCTTGT |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
RefSeq | XP_006717528.1 |
CDS | 2596..4716 |
Translation |

Target ORF information:
Target ORF information:
|
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ORF Insert Sequence:
1 | ATGTCGCCAT TTCTTCGGAT TGGCTTGTCC AACTTTGACT GCGGGTCCTG CCAGTCTTGT |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
CloneID | OHu20978![]() |
|
Clone ID Related Accession (Same CDS sequence) | XM_006717465.3 , XM_024448076.1 , NM_006257.5 , XM_006717465.2 , XM_011519547.1 , XM_017016410.1 , NM_006257.4 , NM_001323265.1 | |
Accession Version | XM_024448076.1 Latest version! | Documents for ORF clone product in default vector |
Sequence Information | ORF Nucleotide Sequence (Length: 2121bp) Protein sequence SNP |
|
Vector | pcDNA3.1+/C-(K)DYK or customized vector | ![]() |
Clone information | Clone Map | ![]() |
Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
ORF Insert Method | CloneEZ™ Seamless cloning technology | |
Insert Structure | linear | |
Update Date | 2019-12-05 | |
Organism | Homo sapiens(human) | |
Product | protein kinase C theta type isoform X2 | |
Comment | Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000010.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## |
1 | ATGTCGCCAT TTCTTCGGAT TGGCTTGTCC AACTTTGACT GCGGGTCCTG CCAGTCTTGT |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
RefSeq | XP_024303844.1 |
CDS | 91..2211 |
Translation |

Target ORF information:
Target ORF information:
|
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ORF Insert Sequence:
1 | ATGTCGCCAT TTCTTCGGAT TGGCTTGTCC AACTTTGACT GCGGGTCCTG CCAGTCTTGT |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
CloneID | OHu20978![]() |
|
Clone ID Related Accession (Same CDS sequence) | XM_006717465.3 , XM_024448076.1 , NM_006257.5 , XM_006717465.2 , XM_011519547.1 , XM_017016410.1 , NM_006257.4 , NM_001323265.1 | |
Accession Version | XM_006717465.2 | Documents for ORF clone product in default vector |
Sequence Information | ORF Nucleotide Sequence (Length: 2121bp) Protein sequence SNP |
|
Vector | pcDNA3.1+/C-(K)DYK or customized vector | ![]() |
Clone information | Clone Map | ![]() |
Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
ORF Insert Method | CloneEZ™ Seamless cloning technology | |
Insert Structure | linear | |
Update Date | 2015-03-11 | |
Organism | Homo sapiens(Human) | |
Product | protein kinase C theta type isoform X1 | |
Comment |
1 | ATGTCGCCAT TTCTTCGGAT TGGCTTGTCC AACTTTGACT GCGGGTCCTG CCAGTCTTGT |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
RefSeq | XP_006717528.1 |
Translation |

Target ORF information:
Target ORF information:
|
![]() |

ORF Insert Sequence:
1 | ATGTCGCCAT TTCTTCGGAT TGGCTTGTCC AACTTTGACT GCGGGTCCTG CCAGTCTTGT |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
CloneID | OHu20978![]() |
|
Clone ID Related Accession (Same CDS sequence) | XM_006717465.3 , XM_024448076.1 , NM_006257.5 , XM_006717465.2 , XM_011519547.1 , XM_017016410.1 , NM_006257.4 , NM_001323265.1 | |
Accession Version | XM_011519547.1 Latest version! | Documents for ORF clone product in default vector |
Sequence Information | ORF Nucleotide Sequence (Length: 2121bp) Protein sequence SNP |
|
Vector | pcDNA3.1+/C-(K)DYK or customized vector | ![]() |
Clone information | Clone Map | ![]() |
Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
ORF Insert Method | CloneEZ™ Seamless cloning technology | |
Insert Structure | linear | |
Update Date | 2015-03-11 | |
Organism | Homo sapiens(Human) | |
Product | protein kinase C theta type isoform X1 | |
Comment |
1 | ATGTCGCCAT TTCTTCGGAT TGGCTTGTCC AACTTTGACT GCGGGTCCTG CCAGTCTTGT |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
RefSeq | XP_011517849.1 |
Translation |

Target ORF information:
Target ORF information:
|
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ORF Insert Sequence:
1 | ATGTCGCCAT TTCTTCGGAT TGGCTTGTCC AACTTTGACT GCGGGTCCTG CCAGTCTTGT |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
CloneID | OHu20978![]() |
|
Clone ID Related Accession (Same CDS sequence) | XM_006717465.3 , XM_024448076.1 , NM_006257.5 , XM_006717465.2 , XM_011519547.1 , XM_017016410.1 , NM_006257.4 , NM_001323265.1 | |
Accession Version | XM_017016410.1 Latest version! | Documents for ORF clone product in default vector |
Sequence Information | ORF Nucleotide Sequence (Length: 2121bp) Protein sequence SNP |
|
Vector | pcDNA3.1+/C-(K)DYK or customized vector | ![]() |
Clone information | Clone Map | ![]() |
Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
ORF Insert Method | CloneEZ™ Seamless cloning technology | |
Insert Structure | linear | |
Update Date | 2016-06-05 | |
Organism | Homo sapiens(human) | |
Product | protein kinase C theta type isoform X2 | |
Comment | Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000010.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## |
1 | ATGTCGCCAT TTCTTCGGAT TGGCTTGTCC AACTTTGACT GCGGGTCCTG CCAGTCTTGT |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
RefSeq | XP_016871899.1 |
CDS | 2507..4627 |
Translation |

Target ORF information:
Target ORF information:
|
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ORF Insert Sequence:
1 | ATGTCGCCAT TTCTTCGGAT TGGCTTGTCC AACTTTGACT GCGGGTCCTG CCAGTCTTGT |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
CloneID | OHu14607 | |
Clone ID Related Accession (Same CDS sequence) | NM_001242413.2 | |
Accession Version | NM_001242413.2 Latest version! | Documents for ORF clone product in default vector |
Sequence Information | ORF Nucleotide Sequence (Length: 1932bp) Protein sequence SNP |
|
Vector | pcDNA3.1+/C-(K)DYK or customized vector | ![]() |
Clone information | Clone Map | ![]() |
Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
ORF Insert Method | CloneEZ™ Seamless cloning technology | |
Insert Structure | linear | |
Update Date | 2019-12-27 | |
Organism | Homo sapiens(human) | |
Product | protein kinase C theta type isoform 2 | |
Comment | Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from L01087.1, AK308099.1, AK312411.1, AY702977.1, AK293935.1, BX647657.1, AA234440.1 and R40214.1. On Sep 17, 2013 this sequence version replaced NM_001242413.1. Transcript Variant: This variant (2) lacks an in-frame exon in the 3' coding region, compared to variant 1. This results in a shorter protein (isoform 2), compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AY702977.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end. |
1 | ATGTCGCCAT TTCTTCGGAT TGGCTTGTCC AACTTTGACT GCGGGTCCTG CCAGTCTTGT |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
RefSeq | NP_001229342.1 |
CDS | 101..2032 |
Translation |

Target ORF information:
Target ORF information:
|
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ORF Insert Sequence:
1 | ATGTCGCCAT TTCTTCGGAT TGGCTTGTCC AACTTTGACT GCGGGTCCTG CCAGTCTTGT |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
CloneID | OHu20385 | |
Clone ID Related Accession (Same CDS sequence) | NM_001323266.2 , XM_024448077.1 , NM_001323266.1 , NM_001282645.1 , XM_017016411.1 | |
Accession Version | NM_001323266.2 Latest version! | Documents for ORF clone product in default vector |
Sequence Information | ORF Nucleotide Sequence (Length: 1746bp) Protein sequence SNP |
|
Vector | pcDNA3.1+/C-(K)DYK or customized vector | ![]() |
Clone information | Clone Map | ![]() |
Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
ORF Insert Method | CloneEZ™ Seamless cloning technology | |
Insert Structure | linear | |
Update Date | 2020-01-03 | |
Organism | Homo sapiens(human) | |
Product | protein kinase C theta type isoform 4 | |
Comment | Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AL158043.14 and AL137145.14. On May 31, 2019 this sequence version replaced NM_001323266.1. Transcript Variant: This variant (6) and variant 4 both encode isoform 4. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803616.144664.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end. |
1 | ATGGACACAA AGGACATGAA TGAATTTGAG ACGGAAGGCT TCTTTGCTTT GCATCAGCGC |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
RefSeq | NP_001310195.1 |
CDS | 388..2133 |
Translation |

Target ORF information:
Target ORF information:
|
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ORF Insert Sequence:
1 | ATGGACACAA AGGACATGAA TGAATTTGAG ACGGAAGGCT TCTTTGCTTT GCATCAGCGC |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
CloneID | OHu20385 | |
Clone ID Related Accession (Same CDS sequence) | NM_001323266.2 , XM_024448077.1 , NM_001323266.1 , NM_001282645.1 , XM_017016411.1 | |
Accession Version | NM_001323266.1 | Documents for ORF clone product in default vector |
Sequence Information | ORF Nucleotide Sequence (Length: 1746bp) Protein sequence SNP |
|
Vector | pcDNA3.1+/C-(K)DYK or customized vector | ![]() |
Clone information | Clone Map | ![]() |
Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
ORF Insert Method | CloneEZ™ Seamless cloning technology | |
Insert Structure | linear | |
Update Date | 2018-09-28 | |
Organism | Homo sapiens(human) | |
Product | protein kinase C theta type isoform 4 | |
Comment | Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AL158043.14 and AL137145.14. Transcript Variant: This variant (6) and variant 4 both encode isoform 4. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## CDS exon combination :: SRR1803616.144664.1, SRR1660807.94444.1 [ECO:0000331] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end. |
1 | ATGGACACAA AGGACATGAA TGAATTTGAG ACGGAAGGCT TCTTTGCTTT GCATCAGCGC |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
RefSeq | NP_001310195.1 |
CDS | 413..2158 |
Translation |

Target ORF information:
Target ORF information:
|
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ORF Insert Sequence:
1 | ATGGACACAA AGGACATGAA TGAATTTGAG ACGGAAGGCT TCTTTGCTTT GCATCAGCGC |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
CloneID | OHu20385 | |
Clone ID Related Accession (Same CDS sequence) | NM_001323266.2 , XM_024448077.1 , NM_001323266.1 , NM_001282645.1 , XM_017016411.1 | |
Accession Version | NM_001282645.1 Latest version! | Documents for ORF clone product in default vector |
Sequence Information | ORF Nucleotide Sequence (Length: 1746bp) Protein sequence SNP |
|
Vector | pcDNA3.1+/C-(K)DYK or customized vector | ![]() |
Clone information | Clone Map | ![]() |
Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
ORF Insert Method | CloneEZ™ Seamless cloning technology | |
Insert Structure | linear | |
Update Date | 2019-12-27 | |
Organism | Homo sapiens(human) | |
Product | protein kinase C theta type isoform 4 | |
Comment | Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from L01087.1, AK308099.1, AK293935.1, BX647657.1, AA234440.1 and R40214.1. On Sep 17, 2013 this sequence version replaced XM_005252499.1. Transcript Variant: This variant (4) lacks an exon in the 5' region. This difference results in translation initiation at an alternate AUG and results in an isoform (4) with a shorter and distinct N-terminus, compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## CDS exon combination :: SRR1660807.94444.1, SRR1803616.144664.1 [ECO:0000331] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end. |
1 | ATGGACACAA AGGACATGAA TGAATTTGAG ACGGAAGGCT TCTTTGCTTT GCATCAGCGC |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
RefSeq | NP_001269574.1 |
CDS | 415..2160 |
Translation |

Target ORF information:
Target ORF information:
|
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ORF Insert Sequence:
1 | ATGGACACAA AGGACATGAA TGAATTTGAG ACGGAAGGCT TCTTTGCTTT GCATCAGCGC |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
CloneID | OHu20385 | |
Clone ID Related Accession (Same CDS sequence) | NM_001323266.2 , XM_024448077.1 , NM_001323266.1 , NM_001282645.1 , XM_017016411.1 | |
Accession Version | XM_024448077.1 Latest version! | Documents for ORF clone product in default vector |
Sequence Information | ORF Nucleotide Sequence (Length: 1746bp) Protein sequence SNP |
|
Vector | pcDNA3.1+/C-(K)DYK or customized vector | ![]() |
Clone information | Clone Map | ![]() |
Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
ORF Insert Method | CloneEZ™ Seamless cloning technology | |
Insert Structure | linear | |
Update Date | 2019-12-05 | |
Organism | Homo sapiens(human) | |
Product | protein kinase C theta type isoform X4 | |
Comment | Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000010.11) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## |
1 | ATGGACACAA AGGACATGAA TGAATTTGAG ACGGAAGGCT TCTTTGCTTT GCATCAGCGC |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
RefSeq | XP_024303845.1 |
CDS | 408..2153 |
Translation |

Target ORF information:
Target ORF information:
|
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ORF Insert Sequence:
1 | ATGGACACAA AGGACATGAA TGAATTTGAG ACGGAAGGCT TCTTTGCTTT GCATCAGCGC |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
CloneID | OHu20385 | |
Clone ID Related Accession (Same CDS sequence) | NM_001323266.2 , XM_024448077.1 , NM_001323266.1 , NM_001282645.1 , XM_017016411.1 | |
Accession Version | XM_017016411.1 Latest version! | Documents for ORF clone product in default vector |
Sequence Information | ORF Nucleotide Sequence (Length: 1746bp) Protein sequence SNP |
|
Vector | pcDNA3.1+/C-(K)DYK or customized vector | ![]() |
Clone information | Clone Map | ![]() |
Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
ORF Insert Method | CloneEZ™ Seamless cloning technology | |
Insert Structure | linear | |
Update Date | 2016-06-05 | |
Organism | Homo sapiens(human) | |
Product | protein kinase C theta type isoform X4 | |
Comment | Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000010.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## |
1 | ATGGACACAA AGGACATGAA TGAATTTGAG ACGGAAGGCT TCTTTGCTTT GCATCAGCGC |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
RefSeq | XP_016871900.1 |
CDS | 2910..4655 |
Translation |

Target ORF information:
Target ORF information:
|
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ORF Insert Sequence:
1 | ATGGACACAA AGGACATGAA TGAATTTGAG ACGGAAGGCT TCTTTGCTTT GCATCAGCGC |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
CloneID | OHu20970 | |
Clone ID Related Accession (Same CDS sequence) | NM_001282644.2 , NM_001282644.1 | |
Accession Version | NM_001282644.2 Latest version! | Documents for ORF clone product in default vector |
Sequence Information | ORF Nucleotide Sequence (Length: 2013bp) Protein sequence SNP |
|
Vector | pcDNA3.1+/C-(K)DYK or customized vector | ![]() |
Clone information | Clone Map | ![]() |
Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
ORF Insert Method | CloneEZ™ Seamless cloning technology | |
Insert Structure | linear | |
Update Date | 2020-01-01 | |
Organism | Homo sapiens(human) | |
Product | protein kinase C theta type isoform 3 | |
Comment | Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AK308099.1, BX647657.1, AA234440.1 and R40214.1. On May 31, 2019 this sequence version replaced NM_001282644.1. Transcript Variant: This variant (3) uses an alternate splice site in the 5' region. This difference results in translation initiation at an alternate AUG and results in an isoform (3) with a shorter and distinct N-terminus, compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK308099.1, SRR1803611.43208.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end. |
1 | ATGTCGAATC AGAACGGGCA GATGTATATC CAGAAAAAGC CTACCATGTA CCCACCCTGG |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
RefSeq | NP_001269573.1 |
CDS | 182..2194 |
Translation |

Target ORF information:
Target ORF information:
|
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ORF Insert Sequence:
1 | ATGTCGAATC AGAACGGGCA GATGTATATC CAGAAAAAGC CTACCATGTA CCCACCCTGG |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
CloneID | OHu20970 | |
Clone ID Related Accession (Same CDS sequence) | NM_001282644.2 , NM_001282644.1 | |
Accession Version | NM_001282644.1 | Documents for ORF clone product in default vector |
Sequence Information | ORF Nucleotide Sequence (Length: 2013bp) Protein sequence SNP |
|
Vector | pcDNA3.1+/C-(K)DYK or customized vector | ![]() |
Clone information | Clone Map | ![]() |
Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
ORF Insert Method | CloneEZ™ Seamless cloning technology | |
Insert Structure | linear | |
Update Date | 2018-09-22 | |
Organism | Homo sapiens(human) | |
Product | protein kinase C theta type isoform 3 | |
Comment | Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from L01087.1, AK308099.1, BX647657.1, AA234440.1 and R40214.1. Transcript Variant: This variant (3) uses an alternate splice site in the 5' region. This difference results in translation initiation at an alternate AUG and results in an isoform (3) with a shorter and distinct N-terminus, compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803613.41376.1, BC143222.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end. |
1 | ATGTCGAATC AGAACGGGCA GATGTATATC CAGAAAAAGC CTACCATGTA CCCACCCTGG |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
RefSeq | NP_001269573.1 |
CDS | 207..2219 |
Translation |

Target ORF information:
Target ORF information:
|
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ORF Insert Sequence:
1 | ATGTCGAATC AGAACGGGCA GATGTATATC CAGAAAAAGC CTACCATGTA CCCACCCTGG |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
CloneID | OHu43782 | |
Clone ID Related Accession (Same CDS sequence) | XM_005252496.4 | |
Accession Version | XM_005252496.4 Latest version! | Documents for ORF clone product in default vector |
Sequence Information | ORF Nucleotide Sequence (Length: 2223bp) Protein sequence SNP |
|
Vector | pcDNA3.1+/C-(K)DYK or customized vector | ![]() |
Clone information | Clone Map | ![]() |
Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
ORF Insert Method | CloneEZ™ Seamless cloning technology | |
Insert Structure | linear | |
Update Date | 2019-12-05 | |
Organism | Homo sapiens(human) | |
Product | protein kinase C theta type isoform X1 | |
Comment | Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000010.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_005252496.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## |
1 | ATGGAACTAA CTCTTGAGTT ACTTCTCAAA AGCAAGGTTG AGTGCAGACT TCCAGAAAGC |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
RefSeq | XP_005252553.1 |
CDS | 99..2321 |
Translation |

Target ORF information:
Target ORF information:
|
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ORF Insert Sequence:
1 | ATGGAACTAA CTCTTGAGTT ACTTCTCAAA AGCAAGGTTG AGTGCAGACT TCCAGAAAGC |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
CloneID | OHu43783 | |
Clone ID Related Accession (Same CDS sequence) | XM_005252497.4 | |
Accession Version | XM_005252497.4 Latest version! | Documents for ORF clone product in default vector |
Sequence Information | ORF Nucleotide Sequence (Length: 2094bp) Protein sequence SNP |
|
Vector | pcDNA3.1+/C-(K)DYK or customized vector | ![]() |
Clone information | Clone Map | ![]() |
Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
ORF Insert Method | CloneEZ™ Seamless cloning technology | |
Insert Structure | linear | |
Update Date | 2019-12-05 | |
Organism | Homo sapiens(human) | |
Product | protein kinase C theta type isoform X3 | |
Comment | Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000010.11) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_005252497.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## |
1 | ATGGAACTAA CTCTTGAGTT ACTTCTCAAA AGCAAGGTTG AGTGCAGACT TCCAGAAAGC |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
RefSeq | XP_005252554.1 |
CDS | 101..2194 |
Translation |

Target ORF information:
Target ORF information:
|
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ORF Insert Sequence:
1 | ATGGAACTAA CTCTTGAGTT ACTTCTCAAA AGCAAGGTTG AGTGCAGACT TCCAGAAAGC |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
CloneID | OHu102085 | |
Clone ID Related Accession (Same CDS sequence) | NM_001323267.1 | |
Accession Version | NM_001323267.1 Latest version! | Documents for ORF clone product in default vector |
Sequence Information | ORF Nucleotide Sequence (Length: 1884bp) Protein sequence SNP |
|
Vector | pcDNA3.1+/C-(K)DYK or customized vector | ![]() |
Clone information | Clone Map | ![]() |
Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
ORF Insert Method | CloneEZ™ Seamless cloning technology | |
Insert Structure | linear | |
Update Date | 2019-12-28 | |
Organism | Homo sapiens(human) | |
Product | protein kinase C theta type isoform 5 | |
Comment | Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AL158043.14 and AL137145.14. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## CDS exon combination :: SRR1803612.63453.1 [ECO:0000331] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## |
1 | ATGTCGAATC AGAACGGGCA GATGTATATC CAGAAAAAGC CTACCATGTA CCCACCCTGG |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
RefSeq | NP_001310196.1 |
CDS | 207..2090 |
Translation |

Target ORF information:
Target ORF information:
|
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ORF Insert Sequence:
1 | ATGTCGAATC AGAACGGGCA GATGTATATC CAGAAAAAGC CTACCATGTA CCCACCCTGG |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
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Structural determinants of phorbol ester binding activity of the C1a and C1b domains of protein kinase C theta. |
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Protein kinase C? controls type 2 innate lymphoid cell and T |
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eNOS S-nitrosylates ?-actin on Cys374 and regulates PKC-? at the immune synapse by impairing actin binding to profilin-1. |
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PKC?-induced phosphorylations control the ability of Fra-1 to stimulate gene expression and cancer cell migration. |