ORF cDNA Clones and Custom Clones » Species Summary » Homo sapiens » PTCH1 cDNA ORF clone

PTCH1 cDNA ORF clone, Homo sapiens(human)

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Summary

Gene Symbol	PTCH1
Entrez Gene ID	5727
Full Name	patched 1
Synonyms	BCNS,NBCCS,PTC,PTC1,PTCH
General protein information	Preferred Names patched 1 Names protein patched homolog 1
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 9 Map Location: 9q22.32
Summary	This gene encodes a member of the patched family of proteins and a component of the hedgehog signaling pathway. Hedgehog signaling is important in embryonic development and tumorigenesis. The encoded protein is the receptor for the secreted hedgehog ligands, which include sonic hedgehog, indian hedgehog and desert hedgehog. Following binding by one of the hedgehog ligands, the encoded protein is trafficked away from the primary cilium, relieving inhibition of the G-protein-coupled receptor smoothened, which results in activation of downstream signaling. Mutations of this gene have been associated with basal cell nevus syndrome and holoprosencephaly. [provided by RefSeq, Aug 2017].
Disorder MIM:	601309
Disorder Html:	Basal cell nevus syndrome, 109400 (3); Basal cell carcinoma, somatic, 605462 (3); Holoprosencephaly 7, 610828 (3)

Reference Sequences (RefSeq)

mRNA and Protein(s)

mRNA	Protein	Name
XM_005252102.2	XP_005252159.1	protein patched homolog 1 isoform X1
XM_011518872.1	XP_011517174.1	protein patched homolog 1 isoform X1
NM_001083605.1	NP_001077074.1	protein patched homolog 1 isoform S
NM_001083603.2	NP_001077072.1	protein patched homolog 1 isoform L'
NM_001083602.2	NP_001077071.1	protein patched homolog 1 isoform M
NM_001083604.2	NP_001077073.1	protein patched homolog 1 isoform S
NM_001354918.1	NP_001341847.1	protein patched homolog 1 isoform 8
NM_001083602.1	NP_001077071.1	protein patched homolog 1 isoform M
NM_001083603.1	NP_001077072.1	protein patched homolog 1 isoform L'
NM_001083606.2	NP_001077075.1	protein patched homolog 1 isoform S
NM_001083606.1	NP_001077075.1	protein patched homolog 1 isoform S
XM_011518871.1	XP_011517173.1	protein patched homolog 1 isoform X1
XM_011518869.1	XP_011517171.1	protein patched homolog 1 isoform X1
NM_001083604.1	NP_001077073.1	protein patched homolog 1 isoform S
NM_001354918.2	NP_001341847.1	protein patched homolog 1 isoform 8
XM_011518870.1	XP_011517172.1	protein patched homolog 1 isoform X1
NM_001083607.1	NP_001077076.1	protein patched homolog 1 isoform S
NM_000264.4	NP_000255.2	protein patched homolog 1 isoform L
NM_001083606.3	NP_001077075.1	protein patched homolog 1 isoform S
NM_001083605.2	NP_001077074.1	protein patched homolog 1 isoform S
NM_000264.3	NP_000255.2	protein patched homolog 1 isoform L
NM_001083607.2	NP_001077076.1	protein patched homolog 1 isoform S
NM_000264.5	NP_000255.2	protein patched homolog 1 isoform L
NM_001354919.1	NP_001341848.1	protein patched homolog 1 isoform 9

Homologies

	PTCH1	XP_001111868.2
Canis lupus familiaris (dog)	PTCH1	XP_003638919.1
Rattus norvegicus (Norway rat)	Ptch1	NP_446018.1
Gallus gallus (chicken)	PTCH1	NP_990291.1
Caenorhabditis elegans (roundworm)	ptc-1	NP_495662.2
Danio rerio (zebrafish)	ptch1	XP_001922161.4
Caenorhabditis elegans (roundworm)	ptc-3	NP_494384.2
Xenopus tropicalis (tropical clawed frog)	ptch1	XP_002935019.2
Pan troglodytes (chimpanzee)	PTCH1	XP_003312241.1
Bos taurus (cattle)	PTCH1	NP_001192808.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP011395	XP_317925.4
Caenorhabditis elegans (roundworm)	ptc-2	NP_495218.2
Mus musculus (house mouse)	Ptch1	NP_032983.1
Homo sapiens (human)	PTCH1	NP_000255.2
Drosophila melanogaster (fruit fly)	ptc	NP_523661.2

Bibliography

Related articles in PubMed

Identification and characterization of two novel PTCH1 splice variants.
Yu P, Yang J, Zhang Y
Biochemical and biophysical research communications487(1)68-75(2017 05)

Hedgehog Signaling: From Basic Biology to Cancer Therapy.
Wu F, Zhang Y, Sun B, McMahon AP, Wang Y
Cell chemical biology24(3)252-280(2017 Mar)

Assessment of Promoter Methylation Identifies PTCH as a Putative Tumor-suppressor Gene in Human CLL.
Schmidt-Wolf IG, Plass C, Byrd JC, Frevel K, Pietsch T, Waha A
Anticancer research36(9)4515-9(2016 09)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Demonstrate altered cytoplasmic expression of Ptc1 and reduced number and length of primary cilia, where Ptc1 is located, in fibroblasts from Niemann-Pick type C patients.
Title: Primary cilium alterations and expression changes of Patched1 proteins in niemann-pick type C disease.

The authors found 15 germline PTCH1 mutations, uniformly distributed across the PTCH1 gene among patients with Basaloid Follicular Hamartoma and Basal Cell Carcinoma. Therefore, Basaloid Follicular Hamartoma and Basal Cell Carcinoma may be the same genetic entity and not two distinctive syndromes.

Thus, our study clearly demonstrated the unique involvement of the two novel PTCH1 splice variants in HH signal transduction.
Title: Identification and characterization of two novel PTCH1 splice variants.

the significant increase of blunt-ended, double-stranded DNA breaks, but not other types of DNA breaks, in normal cells from patients with RET/PTC-driven tumors suggests that blunt-ended double-stranded DNA breaks are a preferred substrate for rearrangement formation, and implicate involvement of the non-homologous end joining pathway in the formation of RET/PTC rearrangements.
Title: DNA fragile site breakage as a measure of chemical exposure and predictor of individual susceptibility to form oncogenic rearrangements.

PTCH1 expression is a promising molecular marker for predicting the imatinib response in chronic myeloid leukemia patients in chronic phase.
Title: PTCH1 is a reliable marker for predicting imatinib response in chronic myeloid leukemia patients in chronic phase.

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The following PTCH1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the PTCH1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

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***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu17265	NM_001083604.2 Latest version!	Homo sapiens patched 1 (PTCH1), transcript variant 1c, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$459.50-$643.30 $919.00
OHu17265	NM_001083605.2 Latest version!	Homo sapiens patched 1 (PTCH1), transcript variant 1c', mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$459.50-$643.30 $919.00
OHu17265	NM_001083606.3 Latest version!	Homo sapiens patched 1 (PTCH1), transcript variant 1d, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$459.50-$643.30 $919.00
OHu17265	NM_001083607.2 Latest version!	Homo sapiens patched 1 (PTCH1), transcript variant 1e, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$459.50-$643.30 $919.00
OHu18800	NM_000264.5 Latest version!	Homo sapiens patched 1 (PTCH1), transcript variant 1b, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$659.50-$923.30 $1319.00
OHu17265	XM_011518869.1 Latest version!	Homo sapiens patched 1 (PTCH1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$459.50-$643.30 $919.00
OHu17265	XM_011518872.1 Latest version!	Homo sapiens patched 1 (PTCH1), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$459.50-$643.30 $919.00
OHu17265	XM_011518871.1 Latest version!	Homo sapiens patched 1 (PTCH1), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$459.50-$643.30 $919.00
OHu17265	XM_005252102.2 Latest version!	Homo sapiens patched 1 (PTCH1), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$459.50-$643.30 $919.00
OHu17265	XM_011518870.1 Latest version!	Homo sapiens patched 1 (PTCH1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$459.50-$643.30 $919.00
OHu102968	NM_001354919.1 Latest version!	Homo sapiens patched 1 (PTCH1), transcript variant 9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$69.30 $99.00
OHu59996	NM_001354918.2 Latest version!	Homo sapiens patched 1 (PTCH1), transcript variant 8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OHu17397	NM_001083603.2 Latest version!	Homo sapiens patched 1 (PTCH1), transcript variant 1a', mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$923.30 $1319.00
OHu19644	NM_001083602.2 Latest version!	Homo sapiens patched 1 (PTCH1), transcript variant 1a, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$923.30 $1319.00
View Old Accession Versions >>
OHu19644	NM_001083602.1	Homo sapiens patched 1 (PTCH1), transcript variant 1a, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$923.30 $1319.00
OHu17397	NM_001083603.1	Homo sapiens patched 1 (PTCH1), transcript variant 1a', mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$923.30 $1319.00
OHu17265	NM_001083606.1	Homo sapiens patched 1 (PTCH1), transcript variant 1d, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$459.50-$643.30 $919.00
OHu17265	NM_001083604.1	Homo sapiens patched 1 (PTCH1), transcript variant 1c, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$459.50-$643.30 $919.00
OHu59996	NM_001354918.1	Homo sapiens patched 1 (PTCH1), transcript variant 8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$727.30 $1039.00
OHu17265	NM_001083606.2	Homo sapiens patched 1 (PTCH1), transcript variant 1d, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$459.50-$643.30 $919.00
OHu17265	NM_001083605.1	Homo sapiens patched 1 (PTCH1), transcript variant 1c', mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$459.50-$643.30 $919.00
OHu18800	NM_000264.3	Homo sapiens patched 1 (PTCH1), transcript variant 1b, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$659.50-$923.30 $1319.00
OHu17265	NM_001083607.1	Homo sapiens patched 1 (PTCH1), transcript variant 1e, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$459.50-$643.30 $919.00
OHu18800	NM_000264.4	Homo sapiens patched 1 (PTCH1), transcript variant 1b, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$659.50-$923.30 $1319.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

PTCH1 ( NM_001083605.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001077074.1 Homo sapiens patched 1 (PTCH1), transcript variant 1c', mRNA.

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CloneID	OHu17265
Clone ID Related Accession (Same CDS sequence)	XM_005252102.2 , XM_011518872.1 , NM_001083605.1 , NM_001083604.2 , NM_001083606.2 , NM_001083606.1 , XM_011518871.1 , XM_011518869.1 , NM_001083604.1 , XM_011518870.1 , NM_001083607.1 , NM_001083606.3 , NM_001083605.2 , NM_001083607.2
Accession Version	NM_001083605.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3891bp) Protein sequence SNP
Vector	pcDNA3.1⁺/C-(K)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-04-24
Organism	Homo sapiens(Human)
Product	protein patched homolog 1 isoform S
Comment	REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AB189439.1, U59464.1, AB209495.1, BX117041.1 and CR744004.1. Transcript Variant: This variant (1c') differs in the 5' UTR and has multiple differences in the 5' coding region, compared to variant 1b. These differences cause translation initiation from a downstream ATG and an isoform (S) with a shorter N-terminus, compared to isoform L. Variants 1c, 1c', 1d, and 1e encode the same isoform (S). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## CDS exon combination :: U43148.1 [ECO:0000331] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGTTTAATC CTCAACTCAT GATACAGACC CCTAAAGAAG AAGGTGCTAA TGTCCTGACC 
ACAGAAGCGC TCCTACAACA CCTGGACTCG GCACTCCAGG CCAGCCGTGT CCATGTATAC 
ATGTACAACA GGCAGTGGAA ATTGGAACAT TTGTGTTACA AATCAGGAGA GCTTATCACA 
GAAACAGGTT ACATGGATCA GATAATAGAA TATCTTTACC CTTGTTTGAT TATTACACCT 
TTGGACTGCT TCTGGGAAGG GGCGAAATTA CAGTCTGGGA CAGCATACCT CCTAGGTAAA 
CCTCCTTTGC GGTGGACAAA CTTCGACCCT TTGGAATTCC TGGAAGAGTT AAAGAAAATA 
AACTATCAAG TGGACAGCTG GGAGGAAATG CTGAATAAGG CTGAGGTTGG TCATGGTTAC 
ATGGACCGCC CCTGCCTCAA TCCGGCCGAT CCAGACTGCC CCGCCACAGC CCCCAACAAA 
AATTCAACCA AACCTCTTGA TATGGCCCTT GTTTTGAATG GTGGATGTCA TGGCTTATCC 
AGAAAGTATA TGCACTGGCA GGAGGAGTTG ATTGTGGGTG GCACAGTCAA GAACAGCACT 
GGAAAACTCG TCAGCGCCCA TGCCCTGCAG ACCATGTTCC AGTTAATGAC TCCCAAGCAA 
ATGTACGAGC ACTTCAAGGG GTACGAGTAT GTCTCACACA TCAACTGGAA CGAGGACAAA 
GCGGCAGCCA TCCTGGAGGC CTGGCAGAGG ACATATGTGG AGGTGGTTCA TCAGAGTGTC 
GCACAGAACT CCACTCAAAA GGTGCTTTCC TTCACCACCA CGACCCTGGA CGACATCCTG 
AAATCCTTCT CTGACGTCAG TGTCATCCGC GTGGCCAGCG GCTACTTACT CATGCTCGCC 
TATGCCTGTC TAACCATGCT GCGCTGGGAC TGCTCCAAGT CCCAGGGTGC CGTGGGGCTG 
GCTGGCGTCC TGCTGGTTGC ACTGTCAGTG GCTGCAGGAC TGGGCCTGTG CTCATTGATC 
GGAATTTCCT TTAACGCTGC AACAACTCAG GTTTTGCCAT TTCTCGCTCT TGGTGTTGGT 
GTGGATGATG TTTTTCTTCT GGCCCACGCC TTCAGTGAAA CAGGACAGAA TAAAAGAATC 
CCTTTTGAGG ACAGGACCGG GGAGTGCCTG AAGCGCACAG GAGCCAGCGT GGCCCTCACG 
TCCATCAGCA ATGTCACAGC CTTCTTCATG GCCGCGTTAA TCCCAATTCC CGCTCTGCGG 
GCGTTCTCCC TCCAGGCAGC GGTAGTAGTG GTGTTCAATT TTGCCATGGT TCTGCTCATT 
TTTCCTGCAA TTCTCAGCAT GGATTTATAT CGACGCGAGG ACAGGAGACT GGATATTTTC 
TGCTGTTTTA CAAGCCCCTG CGTCAGCAGA GTGATTCAGG TTGAACCTCA GGCCTACACC 
GACACACACG ACAATACCCG CTACAGCCCC CCACCTCCCT ACAGCAGCCA CAGCTTTGCC 
CATGAAACGC AGATTACCAT GCAGTCCACT GTCCAGCTCC GCACGGAGTA CGACCCCCAC 
ACGCACGTGT ACTACACCAC CGCTGAGCCG CGCTCCGAGA TCTCTGTGCA GCCCGTCACC 
GTGACACAGG ACACCCTCAG CTGCCAGAGC CCAGAGAGCA CCAGCTCCAC AAGGGACCTG 
CTCTCCCAGT TCTCCGACTC CAGCCTCCAC TGCCTCGAGC CCCCCTGTAC GAAGTGGACA 
CTCTCATCTT TTGCTGAGAA GCACTATGCT CCTTTCCTCT TGAAACCAAA AGCCAAGGTA 
GTGGTGATCT TCCTTTTTCT GGGCTTGCTG GGGGTCAGCC TTTATGGCAC CACCCGAGTG 
AGAGACGGGC TGGACCTTAC GGACATTGTA CCTCGGGAAA CCAGAGAATA TGACTTTATT 
GCTGCACAAT TCAAATACTT TTCTTTCTAC AACATGTATA TAGTCACCCA GAAAGCAGAC 
TACCCGAATA TCCAGCACTT ACTTTACGAC CTACACAGGA GTTTCAGTAA CGTGAAGTAT 
GTCATGTTGG AAGAAAACAA ACAGCTTCCC AAAATGTGGC TGCACTACTT CAGAGACTGG 
CTTCAGGGAC TTCAGGATGC ATTTGACAGT GACTGGGAAA CCGGGAAAAT CATGCCAAAC 
AATTACAAGA ATGGATCAGA CGATGGAGTC CTTGCCTACA AACTCCTGGT GCAAACCGGC 
AGCCGCGATA AGCCCATCGA CATCAGCCAG TTGACTAAAC AGCGTCTGGT GGATGCAGAT 
GGCATCATTA ATCCCAGCGC TTTCTACATC TACCTGACGG CTTGGGTCAG CAACGACCCC 
GTCGCGTATG CTGCCTCCCA GGCCAACATC CGGCCACACC GACCAGAATG GGTCCACGAC 
AAAGCCGACT ACATGCCTGA AACAAGGCTG AGAATCCCGG CAGCAGAGCC CATCGAGTAT 
GCCCAGTTCC CTTTCTACCT CAACGGCTTG CGGGACACCT CAGACTTTGT GGAGGCAATT 
GAAAAAGTAA GGACCATCTG CAGCAACTAT ACGAGCCTGG GGCTGTCCAG TTACCCCAAC 
GGCTACCCCT TCCTCTTCTG GGAGCAGTAC ATCGGCCTCC GCCACTGGCT GCTGCTGTTC 
ATCAGCGTGG TGTTGGCCTG CACATTCCTC GTGTGCGCTG TCTTCCTTCT GAACCCCTGG 
ACGGCCGGGA TCATTGTGAT GGTCCTGGCG CTGATGACGG TCGAGCTGTT CGGCATGATG 
GGCCTCATCG GAATCAAGCT CAGTGCCGTG CCCGTGGTCA TCCTGATCGC TTCTGTTGGC 
ATAGGAGTGG AGTTCACCGT TCACGTTGCT TTGGCCTTTC TGACGGCCAT CGGCGACAAG 
AACCGCAGGG CTGTGCTTGC CCTGGAGCAC ATGTTTGCAC CCGTCCTGGA TGGCGCCGTG 
TCCACTCTGC TGGGAGTGCT GATGCTGGCG GGATCTGAGT TCGACTTCAT TGTCAGGTAT 
TTCTTTGCTG TGCTGGCGAT CCTCACCATC CTCGGCGTTC TCAATGGGCT GGTTTTGCTT 
CCCGTGCTTT TGTCTTTCTT TGGACCATAT CCTGAGGTGT CTCCAGCCAA CGGCTTGAAC 
CGCCTGCCCA CACCCTCCCC TGAGCCACCC CCCAGCGTGG TCCGCTTCGC CATGCCGCCC 
GGCCACACGC ACAGCGGGTC TGATTCCTCC GACTCGGAGT ATAGTTCCCA GACGACAGTG 
TCAGGCCTCA GCGAGGAGCT TCGGCACTAC GAGGCCCAGC AGGGCGCGGG AGGCCCTGCC 
CACCAAGTGA TCGTGGAAGC CACAGAAAAC CCCGTCTTCG CCCACTCCAC TGTGGTCCAT 
CCCGAATCCA GGCATCACCC ACCCTCGAAC CCGAGACAGC AGCCCCACCT GGACTCAGGG 
TCCCTGCCTC CCGGACGGCA AGGCCAGCAG CCCCGCAGGG ACCCCCCCAG AGAAGGCTTG 
TGGCCACCCC CCTACAGACC GCGCAGAGAC GCTTTTGAAA TTTCTACTGA AGGGCATTCT 
GGCCCTAGCA ATAGGGCCCG CTGGGGCCCT CGCGGGGCCC GTTCTCACAA CCCTCGGAAC 
CCAGCGTCCA CTGCCATGGG CAGCTCCGTG CCCGGCTACT GCCAGCCCAT CACCACTGTG 
ACGGCTTCTG CCTCCGTGAC TGTCGCCGTG CACCCGCCGC CTGTCCCTGG GCCTGGGCGG 
AACCCCCGAG GGGGACTCTG CCCAGGCTAC CCTGAGACTG ACCACGGCCT GTTTGAGGAC 
CCCCACGTGC CTTTCCACGT CCGGTGTGAG AGGAGGGATT CGAAGGTGGA AGTCATTGAG 
CTGCAGGACG TGGAATGCGA GGAGAGGCCC CGGGGAAGCA GCTCCAACTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001077074.1
CDS	343..4233
Translation	MFNPQLMIQTPKEEGANVLTTEALLQHLDSALQASRVHVYMYNRQWKLEHLCYKSGELITETGYMDQIIEYLYPCLIITPLDCFWEGAKLQSGTAYLLGKPPLRWTNFDPLEFLEELKKINYQVDSWEEMLNKAEVGHGYMDRPCLNPADPDCPATAPNKNSTKPLDMALVLNGGCHGLSRKYMHWQEELIVGGTVKNSTGKLVSAHALQTMFQLMTPKQMYEHFKGYEYVSHINWNEDKAAAILEAWQRTYVEVVHQSVAQNSTQKVLSFTTTTLDDILKSFSDVSVIRVASGYLLMLAYACLTMLRWDCSKSQGAVGLAGVLLVALSVAAGLGLCSLIGISFNAATTQVLPFLALGVGVDDVFLLAHAFSETGQNKRIPFEDRTGECLKRTGASVALTSISNVTAFFMAALIPIPALRAFSLQAAVVVVFNFAMVLLIFPAILSMDLYRREDRRLDIFCCFTSPCVSRVIQVEPQAYTDTHDNTRYSPPPPYSSHSFAHETQITMQSTVQLRTEYDPHTHVYYTTAEPRSEISVQPVTVTQDTLSCQSPESTSSTRDLLSQFSDSSLHCLEPPCTKWTLSSFAEKHYAPFLLKPKAKVVVIFLFLGLLGVSLYGTTRVRDGLDLTDIVPRETREYDFIAAQFKYFSFYNMYIVTQKADYPNIQHLLYDLHRSFSNVKYVMLEENKQLPKMWLHYFRDWLQGLQDAFDSDWETGKIMPNNYKNGSDDGVLAYKLLVQTGSRDKPIDISQLTKQRLVDADGIINPSAFYIYLTAWVSNDPVAYAASQANIRPHRPEWVHDKADYMPETRLRIPAAEPIEYAQFPFYLNGLRDTSDFVEAIEKVRTICSNYTSLGLSSYPNGYPFLFWEQYIGLRHWLLLFISVVLACTFLVCAVFLLNPWTAGIIVMVLALMTVELFGMMGLIGIKLSAVPVVILIASVGIGVEFTVHVALAFLTAIGDKNRRAVLALEHMFAPVLDGAVSTLLGVLMLAGSEFDFIVRYFFAVLAILTILGVLNGLVLLPVLLSFFGPYPEVSPANGLNRLPTPSPEPPPSVVRFAMPPGHTHSGSDSSDSEYSSQTTVSGLSEELRHYEAQQGAGGPAHQVIVEATENPVFAHSTVVHPESRHHPPSNPRQQPHLDSGSLPPGRQGQQPRRDPPREGLWPPPYRPRRDAFEISTEGHSGPSNRARWGPRGARSHNPRNPASTAMGSSVPGYCQPITTVTASASVTVAVHPPPVPGPGRNPRGGLCPGYPETDHGLFEDPHVPFHVRCERRDSKVEVIELQDVECEERPRGSSSN

Target ORF information:

RefSeq Version	NM_001083605.1
Organism	Homo sapiens(Human)
Definition	Homo sapiens patched 1 (PTCH1), transcript variant 1c', mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001083605.1

ORF Insert Sequence:

ATGTTTAATC CTCAACTCAT GATACAGACC CCTAAAGAAG AAGGTGCTAA TGTCCTGACC 
ACAGAAGCGC TCCTACAACA CCTGGACTCG GCACTCCAGG CCAGCCGTGT CCATGTATAC 
ATGTACAACA GGCAGTGGAA ATTGGAACAT TTGTGTTACA AATCAGGAGA GCTTATCACA 
GAAACAGGTT ACATGGATCA GATAATAGAA TATCTTTACC CTTGTTTGAT TATTACACCT 
TTGGACTGCT TCTGGGAAGG GGCGAAATTA CAGTCTGGGA CAGCATACCT CCTAGGTAAA 
CCTCCTTTGC GGTGGACAAA CTTCGACCCT TTGGAATTCC TGGAAGAGTT AAAGAAAATA 
AACTATCAAG TGGACAGCTG GGAGGAAATG CTGAATAAGG CTGAGGTTGG TCATGGTTAC 
ATGGACCGCC CCTGCCTCAA TCCGGCCGAT CCAGACTGCC CCGCCACAGC CCCCAACAAA 
AATTCAACCA AACCTCTTGA TATGGCCCTT GTTTTGAATG GTGGATGTCA TGGCTTATCC 
AGAAAGTATA TGCACTGGCA GGAGGAGTTG ATTGTGGGTG GCACAGTCAA GAACAGCACT 
GGAAAACTCG TCAGCGCCCA TGCCCTGCAG ACCATGTTCC AGTTAATGAC TCCCAAGCAA 
ATGTACGAGC ACTTCAAGGG GTACGAGTAT GTCTCACACA TCAACTGGAA CGAGGACAAA 
GCGGCAGCCA TCCTGGAGGC CTGGCAGAGG ACATATGTGG AGGTGGTTCA TCAGAGTGTC 
GCACAGAACT CCACTCAAAA GGTGCTTTCC TTCACCACCA CGACCCTGGA CGACATCCTG 
AAATCCTTCT CTGACGTCAG TGTCATCCGC GTGGCCAGCG GCTACTTACT CATGCTCGCC 
TATGCCTGTC TAACCATGCT GCGCTGGGAC TGCTCCAAGT CCCAGGGTGC CGTGGGGCTG 
GCTGGCGTCC TGCTGGTTGC ACTGTCAGTG GCTGCAGGAC TGGGCCTGTG CTCATTGATC 
GGAATTTCCT TTAACGCTGC AACAACTCAG GTTTTGCCAT TTCTCGCTCT TGGTGTTGGT 
GTGGATGATG TTTTTCTTCT GGCCCACGCC TTCAGTGAAA CAGGACAGAA TAAAAGAATC 
CCTTTTGAGG ACAGGACCGG GGAGTGCCTG AAGCGCACAG GAGCCAGCGT GGCCCTCACG 
TCCATCAGCA ATGTCACAGC CTTCTTCATG GCCGCGTTAA TCCCAATTCC CGCTCTGCGG 
GCGTTCTCCC TCCAGGCAGC GGTAGTAGTG GTGTTCAATT TTGCCATGGT TCTGCTCATT 
TTTCCTGCAA TTCTCAGCAT GGATTTATAT CGACGCGAGG ACAGGAGACT GGATATTTTC 
TGCTGTTTTA CAAGCCCCTG CGTCAGCAGA GTGATTCAGG TTGAACCTCA GGCCTACACC 
GACACACACG ACAATACCCG CTACAGCCCC CCACCTCCCT ACAGCAGCCA CAGCTTTGCC 
CATGAAACGC AGATTACCAT GCAGTCCACT GTCCAGCTCC GCACGGAGTA CGACCCCCAC 
ACGCACGTGT ACTACACCAC CGCTGAGCCG CGCTCCGAGA TCTCTGTGCA GCCCGTCACC 
GTGACACAGG ACACCCTCAG CTGCCAGAGC CCAGAGAGCA CCAGCTCCAC AAGGGACCTG 
CTCTCCCAGT TCTCCGACTC CAGCCTCCAC TGCCTCGAGC CCCCCTGTAC GAAGTGGACA 
CTCTCATCTT TTGCTGAGAA GCACTATGCT CCTTTCCTCT TGAAACCAAA AGCCAAGGTA 
GTGGTGATCT TCCTTTTTCT GGGCTTGCTG GGGGTCAGCC TTTATGGCAC CACCCGAGTG 
AGAGACGGGC TGGACCTTAC GGACATTGTA CCTCGGGAAA CCAGAGAATA TGACTTTATT 
GCTGCACAAT TCAAATACTT TTCTTTCTAC AACATGTATA TAGTCACCCA GAAAGCAGAC 
TACCCGAATA TCCAGCACTT ACTTTACGAC CTACACAGGA GTTTCAGTAA CGTGAAGTAT 
GTCATGTTGG AAGAAAACAA ACAGCTTCCC AAAATGTGGC TGCACTACTT CAGAGACTGG 
CTTCAGGGAC TTCAGGATGC ATTTGACAGT GACTGGGAAA CCGGGAAAAT CATGCCAAAC 
AATTACAAGA ATGGATCAGA CGATGGAGTC CTTGCCTACA AACTCCTGGT GCAAACCGGC 
AGCCGCGATA AGCCCATCGA CATCAGCCAG TTGACTAAAC AGCGTCTGGT GGATGCAGAT 
GGCATCATTA ATCCCAGCGC TTTCTACATC TACCTGACGG CTTGGGTCAG CAACGACCCC 
GTCGCGTATG CTGCCTCCCA GGCCAACATC CGGCCACACC GACCAGAATG GGTCCACGAC 
AAAGCCGACT ACATGCCTGA AACAAGGCTG AGAATCCCGG CAGCAGAGCC CATCGAGTAT 
GCCCAGTTCC CTTTCTACCT CAACGGCTTG CGGGACACCT CAGACTTTGT GGAGGCAATT 
GAAAAAGTAA GGACCATCTG CAGCAACTAT ACGAGCCTGG GGCTGTCCAG TTACCCCAAC 
GGCTACCCCT TCCTCTTCTG GGAGCAGTAC ATCGGCCTCC GCCACTGGCT GCTGCTGTTC 
ATCAGCGTGG TGTTGGCCTG CACATTCCTC GTGTGCGCTG TCTTCCTTCT GAACCCCTGG 
ACGGCCGGGA TCATTGTGAT GGTCCTGGCG CTGATGACGG TCGAGCTGTT CGGCATGATG 
GGCCTCATCG GAATCAAGCT CAGTGCCGTG CCCGTGGTCA TCCTGATCGC TTCTGTTGGC 
ATAGGAGTGG AGTTCACCGT TCACGTTGCT TTGGCCTTTC TGACGGCCAT CGGCGACAAG 
AACCGCAGGG CTGTGCTTGC CCTGGAGCAC ATGTTTGCAC CCGTCCTGGA TGGCGCCGTG 
TCCACTCTGC TGGGAGTGCT GATGCTGGCG GGATCTGAGT TCGACTTCAT TGTCAGGTAT 
TTCTTTGCTG TGCTGGCGAT CCTCACCATC CTCGGCGTTC TCAATGGGCT GGTTTTGCTT 
CCCGTGCTTT TGTCTTTCTT TGGACCATAT CCTGAGGTGT CTCCAGCCAA CGGCTTGAAC 
CGCCTGCCCA CACCCTCCCC TGAGCCACCC CCCAGCGTGG TCCGCTTCGC CATGCCGCCC 
GGCCACACGC ACAGCGGGTC TGATTCCTCC GACTCGGAGT ATAGTTCCCA GACGACAGTG 
TCAGGCCTCA GCGAGGAGCT TCGGCACTAC GAGGCCCAGC AGGGCGCGGG AGGCCCTGCC 
CACCAAGTGA TCGTGGAAGC CACAGAAAAC CCCGTCTTCG CCCACTCCAC TGTGGTCCAT 
CCCGAATCCA GGCATCACCC ACCCTCGAAC CCGAGACAGC AGCCCCACCT GGACTCAGGG 
TCCCTGCCTC CCGGACGGCA AGGCCAGCAG CCCCGCAGGG ACCCCCCCAG AGAAGGCTTG 
TGGCCACCCC CCTACAGACC GCGCAGAGAC GCTTTTGAAA TTTCTACTGA AGGGCATTCT 
GGCCCTAGCA ATAGGGCCCG CTGGGGCCCT CGCGGGGCCC GTTCTCACAA CCCTCGGAAC 
CCAGCGTCCA CTGCCATGGG CAGCTCCGTG CCCGGCTACT GCCAGCCCAT CACCACTGTG 
ACGGCTTCTG CCTCCGTGAC TGTCGCCGTG CACCCGCCGC CTGTCCCTGG GCCTGGGCGG 
AACCCCCGAG GGGGACTCTG CCCAGGCTAC CCTGAGACTG ACCACGGCCT GTTTGAGGAC 
CCCCACGTGC CTTTCCACGT CCGGTGTGAG AGGAGGGATT CGAAGGTGGA AGTCATTGAG 
CTGCAGGACG TGGAATGCGA GGAGAGGCCC CGGGGAAGCA GCTCCAACTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PTCH1 ( NM_001083604.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001077073.1 Homo sapiens patched 1 (PTCH1), transcript variant 1c, mRNA.

Price & Availability↑

CloneID	OHu17265
Clone ID Related Accession (Same CDS sequence)	XM_005252102.2 , XM_011518872.1 , NM_001083605.1 , NM_001083604.2 , NM_001083606.2 , NM_001083606.1 , XM_011518871.1 , XM_011518869.1 , NM_001083604.1 , XM_011518870.1 , NM_001083607.1 , NM_001083606.3 , NM_001083605.2 , NM_001083607.2
Accession Version	NM_001083604.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3891bp) Protein sequence SNP
Vector	pcDNA3.1⁺/C-(K)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-22
Organism	Homo sapiens(human)
Product	protein patched homolog 1 isoform S
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AB189438.1, U59464.1, AB209495.1, BX117041.1, CR744004.1 and AI417736.1. On Aug 29, 2017 this sequence version replaced NM_001083604.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## CDS exon combination :: U43148.1 [ECO:0000331] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGTTTAATC CTCAACTCAT GATACAGACC CCTAAAGAAG AAGGTGCTAA TGTCCTGACC 
ACAGAAGCGC TCCTACAACA CCTGGACTCG GCACTCCAGG CCAGCCGTGT CCATGTATAC 
ATGTACAACA GGCAGTGGAA ATTGGAACAT TTGTGTTACA AATCAGGAGA GCTTATCACA 
GAAACAGGTT ACATGGATCA GATAATAGAA TATCTTTACC CTTGTTTGAT TATTACACCT 
TTGGACTGCT TCTGGGAAGG GGCGAAATTA CAGTCTGGGA CAGCATACCT CCTAGGTAAA 
CCTCCTTTGC GGTGGACAAA CTTCGACCCT TTGGAATTCC TGGAAGAGTT AAAGAAAATA 
AACTATCAAG TGGACAGCTG GGAGGAAATG CTGAATAAGG CTGAGGTTGG TCATGGTTAC 
ATGGACCGCC CCTGCCTCAA TCCGGCCGAT CCAGACTGCC CCGCCACAGC CCCCAACAAA 
AATTCAACCA AACCTCTTGA TATGGCCCTT GTTTTGAATG GTGGATGTCA TGGCTTATCC 
AGAAAGTATA TGCACTGGCA GGAGGAGTTG ATTGTGGGTG GCACAGTCAA GAACAGCACT 
GGAAAACTCG TCAGCGCCCA TGCCCTGCAG ACCATGTTCC AGTTAATGAC TCCCAAGCAA 
ATGTACGAGC ACTTCAAGGG GTACGAGTAT GTCTCACACA TCAACTGGAA CGAGGACAAA 
GCGGCAGCCA TCCTGGAGGC CTGGCAGAGG ACATATGTGG AGGTGGTTCA TCAGAGTGTC 
GCACAGAACT CCACTCAAAA GGTGCTTTCC TTCACCACCA CGACCCTGGA CGACATCCTG 
AAATCCTTCT CTGACGTCAG TGTCATCCGC GTGGCCAGCG GCTACTTACT CATGCTCGCC 
TATGCCTGTC TAACCATGCT GCGCTGGGAC TGCTCCAAGT CCCAGGGTGC CGTGGGGCTG 
GCTGGCGTCC TGCTGGTTGC ACTGTCAGTG GCTGCAGGAC TGGGCCTGTG CTCATTGATC 
GGAATTTCCT TTAACGCTGC AACAACTCAG GTTTTGCCAT TTCTCGCTCT TGGTGTTGGT 
GTGGATGATG TTTTTCTTCT GGCCCACGCC TTCAGTGAAA CAGGACAGAA TAAAAGAATC 
CCTTTTGAGG ACAGGACCGG GGAGTGCCTG AAGCGCACAG GAGCCAGCGT GGCCCTCACG 
TCCATCAGCA ATGTCACAGC CTTCTTCATG GCCGCGTTAA TCCCAATTCC CGCTCTGCGG 
GCGTTCTCCC TCCAGGCAGC GGTAGTAGTG GTGTTCAATT TTGCCATGGT TCTGCTCATT 
TTTCCTGCAA TTCTCAGCAT GGATTTATAT CGACGCGAGG ACAGGAGACT GGATATTTTC 
TGCTGTTTTA CAAGCCCCTG CGTCAGCAGA GTGATTCAGG TTGAACCTCA GGCCTACACC 
GACACACACG ACAATACCCG CTACAGCCCC CCACCTCCCT ACAGCAGCCA CAGCTTTGCC 
CATGAAACGC AGATTACCAT GCAGTCCACT GTCCAGCTCC GCACGGAGTA CGACCCCCAC 
ACGCACGTGT ACTACACCAC CGCTGAGCCG CGCTCCGAGA TCTCTGTGCA GCCCGTCACC 
GTGACACAGG ACACCCTCAG CTGCCAGAGC CCAGAGAGCA CCAGCTCCAC AAGGGACCTG 
CTCTCCCAGT TCTCCGACTC CAGCCTCCAC TGCCTCGAGC CCCCCTGTAC GAAGTGGACA 
CTCTCATCTT TTGCTGAGAA GCACTATGCT CCTTTCCTCT TGAAACCAAA AGCCAAGGTA 
GTGGTGATCT TCCTTTTTCT GGGCTTGCTG GGGGTCAGCC TTTATGGCAC CACCCGAGTG 
AGAGACGGGC TGGACCTTAC GGACATTGTA CCTCGGGAAA CCAGAGAATA TGACTTTATT 
GCTGCACAAT TCAAATACTT TTCTTTCTAC AACATGTATA TAGTCACCCA GAAAGCAGAC 
TACCCGAATA TCCAGCACTT ACTTTACGAC CTACACAGGA GTTTCAGTAA CGTGAAGTAT 
GTCATGTTGG AAGAAAACAA ACAGCTTCCC AAAATGTGGC TGCACTACTT CAGAGACTGG 
CTTCAGGGAC TTCAGGATGC ATTTGACAGT GACTGGGAAA CCGGGAAAAT CATGCCAAAC 
AATTACAAGA ATGGATCAGA CGATGGAGTC CTTGCCTACA AACTCCTGGT GCAAACCGGC 
AGCCGCGATA AGCCCATCGA CATCAGCCAG TTGACTAAAC AGCGTCTGGT GGATGCAGAT 
GGCATCATTA ATCCCAGCGC TTTCTACATC TACCTGACGG CTTGGGTCAG CAACGACCCC 
GTCGCGTATG CTGCCTCCCA GGCCAACATC CGGCCACACC GACCAGAATG GGTCCACGAC 
AAAGCCGACT ACATGCCTGA AACAAGGCTG AGAATCCCGG CAGCAGAGCC CATCGAGTAT 
GCCCAGTTCC CTTTCTACCT CAACGGCTTG CGGGACACCT CAGACTTTGT GGAGGCAATT 
GAAAAAGTAA GGACCATCTG CAGCAACTAT ACGAGCCTGG GGCTGTCCAG TTACCCCAAC 
GGCTACCCCT TCCTCTTCTG GGAGCAGTAC ATCGGCCTCC GCCACTGGCT GCTGCTGTTC 
ATCAGCGTGG TGTTGGCCTG CACATTCCTC GTGTGCGCTG TCTTCCTTCT GAACCCCTGG 
ACGGCCGGGA TCATTGTGAT GGTCCTGGCG CTGATGACGG TCGAGCTGTT CGGCATGATG 
GGCCTCATCG GAATCAAGCT CAGTGCCGTG CCCGTGGTCA TCCTGATCGC TTCTGTTGGC 
ATAGGAGTGG AGTTCACCGT TCACGTTGCT TTGGCCTTTC TGACGGCCAT CGGCGACAAG 
AACCGCAGGG CTGTGCTTGC CCTGGAGCAC ATGTTTGCAC CCGTCCTGGA TGGCGCCGTG 
TCCACTCTGC TGGGAGTGCT GATGCTGGCG GGATCTGAGT TCGACTTCAT TGTCAGGTAT 
TTCTTTGCTG TGCTGGCGAT CCTCACCATC CTCGGCGTTC TCAATGGGCT GGTTTTGCTT 
CCCGTGCTTT TGTCTTTCTT TGGACCATAT CCTGAGGTGT CTCCAGCCAA CGGCTTGAAC 
CGCCTGCCCA CACCCTCCCC TGAGCCACCC CCCAGCGTGG TCCGCTTCGC CATGCCGCCC 
GGCCACACGC ACAGCGGGTC TGATTCCTCC GACTCGGAGT ATAGTTCCCA GACGACAGTG 
TCAGGCCTCA GCGAGGAGCT TCGGCACTAC GAGGCCCAGC AGGGCGCGGG AGGCCCTGCC 
CACCAAGTGA TCGTGGAAGC CACAGAAAAC CCCGTCTTCG CCCACTCCAC TGTGGTCCAT 
CCCGAATCCA GGCATCACCC ACCCTCGAAC CCGAGACAGC AGCCCCACCT GGACTCAGGG 
TCCCTGCCTC CCGGACGGCA AGGCCAGCAG CCCCGCAGGG ACCCCCCCAG AGAAGGCTTG 
TGGCCACCCC CCTACAGACC GCGCAGAGAC GCTTTTGAAA TTTCTACTGA AGGGCATTCT 
GGCCCTAGCA ATAGGGCCCG CTGGGGCCCT CGCGGGGCCC GTTCTCACAA CCCTCGGAAC 
CCAGCGTCCA CTGCCATGGG CAGCTCCGTG CCCGGCTACT GCCAGCCCAT CACCACTGTG 
ACGGCTTCTG CCTCCGTGAC TGTCGCCGTG CACCCGCCGC CTGTCCCTGG GCCTGGGCGG 
AACCCCCGAG GGGGACTCTG CCCAGGCTAC CCTGAGACTG ACCACGGCCT GTTTGAGGAC 
CCCCACGTGC CTTTCCACGT CCGGTGTGAG AGGAGGGATT CGAAGGTGGA AGTCATTGAG 
CTGCAGGACG TGGAATGCGA GGAGAGGCCC CGGGGAAGCA GCTCCAACTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001077073.1
CDS	360..4250
Translation	MFNPQLMIQTPKEEGANVLTTEALLQHLDSALQASRVHVYMYNRQWKLEHLCYKSGELITETGYMDQIIEYLYPCLIITPLDCFWEGAKLQSGTAYLLGKPPLRWTNFDPLEFLEELKKINYQVDSWEEMLNKAEVGHGYMDRPCLNPADPDCPATAPNKNSTKPLDMALVLNGGCHGLSRKYMHWQEELIVGGTVKNSTGKLVSAHALQTMFQLMTPKQMYEHFKGYEYVSHINWNEDKAAAILEAWQRTYVEVVHQSVAQNSTQKVLSFTTTTLDDILKSFSDVSVIRVASGYLLMLAYACLTMLRWDCSKSQGAVGLAGVLLVALSVAAGLGLCSLIGISFNAATTQVLPFLALGVGVDDVFLLAHAFSETGQNKRIPFEDRTGECLKRTGASVALTSISNVTAFFMAALIPIPALRAFSLQAAVVVVFNFAMVLLIFPAILSMDLYRREDRRLDIFCCFTSPCVSRVIQVEPQAYTDTHDNTRYSPPPPYSSHSFAHETQITMQSTVQLRTEYDPHTHVYYTTAEPRSEISVQPVTVTQDTLSCQSPESTSSTRDLLSQFSDSSLHCLEPPCTKWTLSSFAEKHYAPFLLKPKAKVVVIFLFLGLLGVSLYGTTRVRDGLDLTDIVPRETREYDFIAAQFKYFSFYNMYIVTQKADYPNIQHLLYDLHRSFSNVKYVMLEENKQLPKMWLHYFRDWLQGLQDAFDSDWETGKIMPNNYKNGSDDGVLAYKLLVQTGSRDKPIDISQLTKQRLVDADGIINPSAFYIYLTAWVSNDPVAYAASQANIRPHRPEWVHDKADYMPETRLRIPAAEPIEYAQFPFYLNGLRDTSDFVEAIEKVRTICSNYTSLGLSSYPNGYPFLFWEQYIGLRHWLLLFISVVLACTFLVCAVFLLNPWTAGIIVMVLALMTVELFGMMGLIGIKLSAVPVVILIASVGIGVEFTVHVALAFLTAIGDKNRRAVLALEHMFAPVLDGAVSTLLGVLMLAGSEFDFIVRYFFAVLAILTILGVLNGLVLLPVLLSFFGPYPEVSPANGLNRLPTPSPEPPPSVVRFAMPPGHTHSGSDSSDSEYSSQTTVSGLSEELRHYEAQQGAGGPAHQVIVEATENPVFAHSTVVHPESRHHPPSNPRQQPHLDSGSLPPGRQGQQPRRDPPREGLWPPPYRPRRDAFEISTEGHSGPSNRARWGPRGARSHNPRNPASTAMGSSVPGYCQPITTVTASASVTVAVHPPPVPGPGRNPRGGLCPGYPETDHGLFEDPHVPFHVRCERRDSKVEVIELQDVECEERPRGSSSN

Target ORF information:

RefSeq Version	NM_001083604.2
Organism	Homo sapiens(human)
Definition	Homo sapiens patched 1 (PTCH1), transcript variant 1c, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001083604.2

ORF Insert Sequence:

ATGTTTAATC CTCAACTCAT GATACAGACC CCTAAAGAAG AAGGTGCTAA TGTCCTGACC 
ACAGAAGCGC TCCTACAACA CCTGGACTCG GCACTCCAGG CCAGCCGTGT CCATGTATAC 
ATGTACAACA GGCAGTGGAA ATTGGAACAT TTGTGTTACA AATCAGGAGA GCTTATCACA 
GAAACAGGTT ACATGGATCA GATAATAGAA TATCTTTACC CTTGTTTGAT TATTACACCT 
TTGGACTGCT TCTGGGAAGG GGCGAAATTA CAGTCTGGGA CAGCATACCT CCTAGGTAAA 
CCTCCTTTGC GGTGGACAAA CTTCGACCCT TTGGAATTCC TGGAAGAGTT AAAGAAAATA 
AACTATCAAG TGGACAGCTG GGAGGAAATG CTGAATAAGG CTGAGGTTGG TCATGGTTAC 
ATGGACCGCC CCTGCCTCAA TCCGGCCGAT CCAGACTGCC CCGCCACAGC CCCCAACAAA 
AATTCAACCA AACCTCTTGA TATGGCCCTT GTTTTGAATG GTGGATGTCA TGGCTTATCC 
AGAAAGTATA TGCACTGGCA GGAGGAGTTG ATTGTGGGTG GCACAGTCAA GAACAGCACT 
GGAAAACTCG TCAGCGCCCA TGCCCTGCAG ACCATGTTCC AGTTAATGAC TCCCAAGCAA 
ATGTACGAGC ACTTCAAGGG GTACGAGTAT GTCTCACACA TCAACTGGAA CGAGGACAAA 
GCGGCAGCCA TCCTGGAGGC CTGGCAGAGG ACATATGTGG AGGTGGTTCA TCAGAGTGTC 
GCACAGAACT CCACTCAAAA GGTGCTTTCC TTCACCACCA CGACCCTGGA CGACATCCTG 
AAATCCTTCT CTGACGTCAG TGTCATCCGC GTGGCCAGCG GCTACTTACT CATGCTCGCC 
TATGCCTGTC TAACCATGCT GCGCTGGGAC TGCTCCAAGT CCCAGGGTGC CGTGGGGCTG 
GCTGGCGTCC TGCTGGTTGC ACTGTCAGTG GCTGCAGGAC TGGGCCTGTG CTCATTGATC 
GGAATTTCCT TTAACGCTGC AACAACTCAG GTTTTGCCAT TTCTCGCTCT TGGTGTTGGT 
GTGGATGATG TTTTTCTTCT GGCCCACGCC TTCAGTGAAA CAGGACAGAA TAAAAGAATC 
CCTTTTGAGG ACAGGACCGG GGAGTGCCTG AAGCGCACAG GAGCCAGCGT GGCCCTCACG 
TCCATCAGCA ATGTCACAGC CTTCTTCATG GCCGCGTTAA TCCCAATTCC CGCTCTGCGG 
GCGTTCTCCC TCCAGGCAGC GGTAGTAGTG GTGTTCAATT TTGCCATGGT TCTGCTCATT 
TTTCCTGCAA TTCTCAGCAT GGATTTATAT CGACGCGAGG ACAGGAGACT GGATATTTTC 
TGCTGTTTTA CAAGCCCCTG CGTCAGCAGA GTGATTCAGG TTGAACCTCA GGCCTACACC 
GACACACACG ACAATACCCG CTACAGCCCC CCACCTCCCT ACAGCAGCCA CAGCTTTGCC 
CATGAAACGC AGATTACCAT GCAGTCCACT GTCCAGCTCC GCACGGAGTA CGACCCCCAC 
ACGCACGTGT ACTACACCAC CGCTGAGCCG CGCTCCGAGA TCTCTGTGCA GCCCGTCACC 
GTGACACAGG ACACCCTCAG CTGCCAGAGC CCAGAGAGCA CCAGCTCCAC AAGGGACCTG 
CTCTCCCAGT TCTCCGACTC CAGCCTCCAC TGCCTCGAGC CCCCCTGTAC GAAGTGGACA 
CTCTCATCTT TTGCTGAGAA GCACTATGCT CCTTTCCTCT TGAAACCAAA AGCCAAGGTA 
GTGGTGATCT TCCTTTTTCT GGGCTTGCTG GGGGTCAGCC TTTATGGCAC CACCCGAGTG 
AGAGACGGGC TGGACCTTAC GGACATTGTA CCTCGGGAAA CCAGAGAATA TGACTTTATT 
GCTGCACAAT TCAAATACTT TTCTTTCTAC AACATGTATA TAGTCACCCA GAAAGCAGAC 
TACCCGAATA TCCAGCACTT ACTTTACGAC CTACACAGGA GTTTCAGTAA CGTGAAGTAT 
GTCATGTTGG AAGAAAACAA ACAGCTTCCC AAAATGTGGC TGCACTACTT CAGAGACTGG 
CTTCAGGGAC TTCAGGATGC ATTTGACAGT GACTGGGAAA CCGGGAAAAT CATGCCAAAC 
AATTACAAGA ATGGATCAGA CGATGGAGTC CTTGCCTACA AACTCCTGGT GCAAACCGGC 
AGCCGCGATA AGCCCATCGA CATCAGCCAG TTGACTAAAC AGCGTCTGGT GGATGCAGAT 
GGCATCATTA ATCCCAGCGC TTTCTACATC TACCTGACGG CTTGGGTCAG CAACGACCCC 
GTCGCGTATG CTGCCTCCCA GGCCAACATC CGGCCACACC GACCAGAATG GGTCCACGAC 
AAAGCCGACT ACATGCCTGA AACAAGGCTG AGAATCCCGG CAGCAGAGCC CATCGAGTAT 
GCCCAGTTCC CTTTCTACCT CAACGGCTTG CGGGACACCT CAGACTTTGT GGAGGCAATT 
GAAAAAGTAA GGACCATCTG CAGCAACTAT ACGAGCCTGG GGCTGTCCAG TTACCCCAAC 
GGCTACCCCT TCCTCTTCTG GGAGCAGTAC ATCGGCCTCC GCCACTGGCT GCTGCTGTTC 
ATCAGCGTGG TGTTGGCCTG CACATTCCTC GTGTGCGCTG TCTTCCTTCT GAACCCCTGG 
ACGGCCGGGA TCATTGTGAT GGTCCTGGCG CTGATGACGG TCGAGCTGTT CGGCATGATG 
GGCCTCATCG GAATCAAGCT CAGTGCCGTG CCCGTGGTCA TCCTGATCGC TTCTGTTGGC 
ATAGGAGTGG AGTTCACCGT TCACGTTGCT TTGGCCTTTC TGACGGCCAT CGGCGACAAG 
AACCGCAGGG CTGTGCTTGC CCTGGAGCAC ATGTTTGCAC CCGTCCTGGA TGGCGCCGTG 
TCCACTCTGC TGGGAGTGCT GATGCTGGCG GGATCTGAGT TCGACTTCAT TGTCAGGTAT 
TTCTTTGCTG TGCTGGCGAT CCTCACCATC CTCGGCGTTC TCAATGGGCT GGTTTTGCTT 
CCCGTGCTTT TGTCTTTCTT TGGACCATAT CCTGAGGTGT CTCCAGCCAA CGGCTTGAAC 
CGCCTGCCCA CACCCTCCCC TGAGCCACCC CCCAGCGTGG TCCGCTTCGC CATGCCGCCC 
GGCCACACGC ACAGCGGGTC TGATTCCTCC GACTCGGAGT ATAGTTCCCA GACGACAGTG 
TCAGGCCTCA GCGAGGAGCT TCGGCACTAC GAGGCCCAGC AGGGCGCGGG AGGCCCTGCC 
CACCAAGTGA TCGTGGAAGC CACAGAAAAC CCCGTCTTCG CCCACTCCAC TGTGGTCCAT 
CCCGAATCCA GGCATCACCC ACCCTCGAAC CCGAGACAGC AGCCCCACCT GGACTCAGGG 
TCCCTGCCTC CCGGACGGCA AGGCCAGCAG CCCCGCAGGG ACCCCCCCAG AGAAGGCTTG 
TGGCCACCCC CCTACAGACC GCGCAGAGAC GCTTTTGAAA TTTCTACTGA AGGGCATTCT 
GGCCCTAGCA ATAGGGCCCG CTGGGGCCCT CGCGGGGCCC GTTCTCACAA CCCTCGGAAC 
CCAGCGTCCA CTGCCATGGG CAGCTCCGTG CCCGGCTACT GCCAGCCCAT CACCACTGTG 
ACGGCTTCTG CCTCCGTGAC TGTCGCCGTG CACCCGCCGC CTGTCCCTGG GCCTGGGCGG 
AACCCCCGAG GGGGACTCTG CCCAGGCTAC CCTGAGACTG ACCACGGCCT GTTTGAGGAC 
CCCCACGTGC CTTTCCACGT CCGGTGTGAG AGGAGGGATT CGAAGGTGGA AGTCATTGAG 
CTGCAGGACG TGGAATGCGA GGAGAGGCCC CGGGGAAGCA GCTCCAACTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PTCH1 ( NM_001083606.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001077075.1 Homo sapiens patched 1 (PTCH1), transcript variant 1d, mRNA.

Price & Availability↑

CloneID	OHu17265
Clone ID Related Accession (Same CDS sequence)	XM_005252102.2 , XM_011518872.1 , NM_001083605.1 , NM_001083604.2 , NM_001083606.2 , NM_001083606.1 , XM_011518871.1 , XM_011518869.1 , NM_001083604.1 , XM_011518870.1 , NM_001083607.1 , NM_001083606.3 , NM_001083605.2 , NM_001083607.2
Accession Version	NM_001083606.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3891bp) Protein sequence SNP
Vector	pcDNA3.1⁺/C-(K)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-25
Organism	Homo sapiens(human)
Product	protein patched homolog 1 isoform S
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from U43148.1, AI358880.1, AB209495.1, BX117041.1, CR744004.1 and AI417736.1. On Aug 29, 2017 this sequence version replaced NM_001083606.1. Transcript Variant: This variant (1d) differs in the 5' UTR and has multiple differences in the 5' coding region, compared to variant 1b. These differences cause translation initiation from a downstream ATG and an isoform (S) with a shorter N-terminus, compared to isoform L. Variants 1c, 1c', 1d, and 1e encode the same isoform (S). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: U43148.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGTTTAATC CTCAACTCAT GATACAGACC CCTAAAGAAG AAGGTGCTAA TGTCCTGACC 
ACAGAAGCGC TCCTACAACA CCTGGACTCG GCACTCCAGG CCAGCCGTGT CCATGTATAC 
ATGTACAACA GGCAGTGGAA ATTGGAACAT TTGTGTTACA AATCAGGAGA GCTTATCACA 
GAAACAGGTT ACATGGATCA GATAATAGAA TATCTTTACC CTTGTTTGAT TATTACACCT 
TTGGACTGCT TCTGGGAAGG GGCGAAATTA CAGTCTGGGA CAGCATACCT CCTAGGTAAA 
CCTCCTTTGC GGTGGACAAA CTTCGACCCT TTGGAATTCC TGGAAGAGTT AAAGAAAATA 
AACTATCAAG TGGACAGCTG GGAGGAAATG CTGAATAAGG CTGAGGTTGG TCATGGTTAC 
ATGGACCGCC CCTGCCTCAA TCCGGCCGAT CCAGACTGCC CCGCCACAGC CCCCAACAAA 
AATTCAACCA AACCTCTTGA TATGGCCCTT GTTTTGAATG GTGGATGTCA TGGCTTATCC 
AGAAAGTATA TGCACTGGCA GGAGGAGTTG ATTGTGGGTG GCACAGTCAA GAACAGCACT 
GGAAAACTCG TCAGCGCCCA TGCCCTGCAG ACCATGTTCC AGTTAATGAC TCCCAAGCAA 
ATGTACGAGC ACTTCAAGGG GTACGAGTAT GTCTCACACA TCAACTGGAA CGAGGACAAA 
GCGGCAGCCA TCCTGGAGGC CTGGCAGAGG ACATATGTGG AGGTGGTTCA TCAGAGTGTC 
GCACAGAACT CCACTCAAAA GGTGCTTTCC TTCACCACCA CGACCCTGGA CGACATCCTG 
AAATCCTTCT CTGACGTCAG TGTCATCCGC GTGGCCAGCG GCTACTTACT CATGCTCGCC 
TATGCCTGTC TAACCATGCT GCGCTGGGAC TGCTCCAAGT CCCAGGGTGC CGTGGGGCTG 
GCTGGCGTCC TGCTGGTTGC ACTGTCAGTG GCTGCAGGAC TGGGCCTGTG CTCATTGATC 
GGAATTTCCT TTAACGCTGC AACAACTCAG GTTTTGCCAT TTCTCGCTCT TGGTGTTGGT 
GTGGATGATG TTTTTCTTCT GGCCCACGCC TTCAGTGAAA CAGGACAGAA TAAAAGAATC 
CCTTTTGAGG ACAGGACCGG GGAGTGCCTG AAGCGCACAG GAGCCAGCGT GGCCCTCACG 
TCCATCAGCA ATGTCACAGC CTTCTTCATG GCCGCGTTAA TCCCAATTCC CGCTCTGCGG 
GCGTTCTCCC TCCAGGCAGC GGTAGTAGTG GTGTTCAATT TTGCCATGGT TCTGCTCATT 
TTTCCTGCAA TTCTCAGCAT GGATTTATAT CGACGCGAGG ACAGGAGACT GGATATTTTC 
TGCTGTTTTA CAAGCCCCTG CGTCAGCAGA GTGATTCAGG TTGAACCTCA GGCCTACACC 
GACACACACG ACAATACCCG CTACAGCCCC CCACCTCCCT ACAGCAGCCA CAGCTTTGCC 
CATGAAACGC AGATTACCAT GCAGTCCACT GTCCAGCTCC GCACGGAGTA CGACCCCCAC 
ACGCACGTGT ACTACACCAC CGCTGAGCCG CGCTCCGAGA TCTCTGTGCA GCCCGTCACC 
GTGACACAGG ACACCCTCAG CTGCCAGAGC CCAGAGAGCA CCAGCTCCAC AAGGGACCTG 
CTCTCCCAGT TCTCCGACTC CAGCCTCCAC TGCCTCGAGC CCCCCTGTAC GAAGTGGACA 
CTCTCATCTT TTGCTGAGAA GCACTATGCT CCTTTCCTCT TGAAACCAAA AGCCAAGGTA 
GTGGTGATCT TCCTTTTTCT GGGCTTGCTG GGGGTCAGCC TTTATGGCAC CACCCGAGTG 
AGAGACGGGC TGGACCTTAC GGACATTGTA CCTCGGGAAA CCAGAGAATA TGACTTTATT 
GCTGCACAAT TCAAATACTT TTCTTTCTAC AACATGTATA TAGTCACCCA GAAAGCAGAC 
TACCCGAATA TCCAGCACTT ACTTTACGAC CTACACAGGA GTTTCAGTAA CGTGAAGTAT 
GTCATGTTGG AAGAAAACAA ACAGCTTCCC AAAATGTGGC TGCACTACTT CAGAGACTGG 
CTTCAGGGAC TTCAGGATGC ATTTGACAGT GACTGGGAAA CCGGGAAAAT CATGCCAAAC 
AATTACAAGA ATGGATCAGA CGATGGAGTC CTTGCCTACA AACTCCTGGT GCAAACCGGC 
AGCCGCGATA AGCCCATCGA CATCAGCCAG TTGACTAAAC AGCGTCTGGT GGATGCAGAT 
GGCATCATTA ATCCCAGCGC TTTCTACATC TACCTGACGG CTTGGGTCAG CAACGACCCC 
GTCGCGTATG CTGCCTCCCA GGCCAACATC CGGCCACACC GACCAGAATG GGTCCACGAC 
AAAGCCGACT ACATGCCTGA AACAAGGCTG AGAATCCCGG CAGCAGAGCC CATCGAGTAT 
GCCCAGTTCC CTTTCTACCT CAACGGCTTG CGGGACACCT CAGACTTTGT GGAGGCAATT 
GAAAAAGTAA GGACCATCTG CAGCAACTAT ACGAGCCTGG GGCTGTCCAG TTACCCCAAC 
GGCTACCCCT TCCTCTTCTG GGAGCAGTAC ATCGGCCTCC GCCACTGGCT GCTGCTGTTC 
ATCAGCGTGG TGTTGGCCTG CACATTCCTC GTGTGCGCTG TCTTCCTTCT GAACCCCTGG 
ACGGCCGGGA TCATTGTGAT GGTCCTGGCG CTGATGACGG TCGAGCTGTT CGGCATGATG 
GGCCTCATCG GAATCAAGCT CAGTGCCGTG CCCGTGGTCA TCCTGATCGC TTCTGTTGGC 
ATAGGAGTGG AGTTCACCGT TCACGTTGCT TTGGCCTTTC TGACGGCCAT CGGCGACAAG 
AACCGCAGGG CTGTGCTTGC CCTGGAGCAC ATGTTTGCAC CCGTCCTGGA TGGCGCCGTG 
TCCACTCTGC TGGGAGTGCT GATGCTGGCG GGATCTGAGT TCGACTTCAT TGTCAGGTAT 
TTCTTTGCTG TGCTGGCGAT CCTCACCATC CTCGGCGTTC TCAATGGGCT GGTTTTGCTT 
CCCGTGCTTT TGTCTTTCTT TGGACCATAT CCTGAGGTGT CTCCAGCCAA CGGCTTGAAC 
CGCCTGCCCA CACCCTCCCC TGAGCCACCC CCCAGCGTGG TCCGCTTCGC CATGCCGCCC 
GGCCACACGC ACAGCGGGTC TGATTCCTCC GACTCGGAGT ATAGTTCCCA GACGACAGTG 
TCAGGCCTCA GCGAGGAGCT TCGGCACTAC GAGGCCCAGC AGGGCGCGGG AGGCCCTGCC 
CACCAAGTGA TCGTGGAAGC CACAGAAAAC CCCGTCTTCG CCCACTCCAC TGTGGTCCAT 
CCCGAATCCA GGCATCACCC ACCCTCGAAC CCGAGACAGC AGCCCCACCT GGACTCAGGG 
TCCCTGCCTC CCGGACGGCA AGGCCAGCAG CCCCGCAGGG ACCCCCCCAG AGAAGGCTTG 
TGGCCACCCC CCTACAGACC GCGCAGAGAC GCTTTTGAAA TTTCTACTGA AGGGCATTCT 
GGCCCTAGCA ATAGGGCCCG CTGGGGCCCT CGCGGGGCCC GTTCTCACAA CCCTCGGAAC 
CCAGCGTCCA CTGCCATGGG CAGCTCCGTG CCCGGCTACT GCCAGCCCAT CACCACTGTG 
ACGGCTTCTG CCTCCGTGAC TGTCGCCGTG CACCCGCCGC CTGTCCCTGG GCCTGGGCGG 
AACCCCCGAG GGGGACTCTG CCCAGGCTAC CCTGAGACTG ACCACGGCCT GTTTGAGGAC 
CCCCACGTGC CTTTCCACGT CCGGTGTGAG AGGAGGGATT CGAAGGTGGA AGTCATTGAG 
CTGCAGGACG TGGAATGCGA GGAGAGGCCC CGGGGAAGCA GCTCCAACTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001077075.1
CDS	442..4332
Translation	MFNPQLMIQTPKEEGANVLTTEALLQHLDSALQASRVHVYMYNRQWKLEHLCYKSGELITETGYMDQIIEYLYPCLIITPLDCFWEGAKLQSGTAYLLGKPPLRWTNFDPLEFLEELKKINYQVDSWEEMLNKAEVGHGYMDRPCLNPADPDCPATAPNKNSTKPLDMALVLNGGCHGLSRKYMHWQEELIVGGTVKNSTGKLVSAHALQTMFQLMTPKQMYEHFKGYEYVSHINWNEDKAAAILEAWQRTYVEVVHQSVAQNSTQKVLSFTTTTLDDILKSFSDVSVIRVASGYLLMLAYACLTMLRWDCSKSQGAVGLAGVLLVALSVAAGLGLCSLIGISFNAATTQVLPFLALGVGVDDVFLLAHAFSETGQNKRIPFEDRTGECLKRTGASVALTSISNVTAFFMAALIPIPALRAFSLQAAVVVVFNFAMVLLIFPAILSMDLYRREDRRLDIFCCFTSPCVSRVIQVEPQAYTDTHDNTRYSPPPPYSSHSFAHETQITMQSTVQLRTEYDPHTHVYYTTAEPRSEISVQPVTVTQDTLSCQSPESTSSTRDLLSQFSDSSLHCLEPPCTKWTLSSFAEKHYAPFLLKPKAKVVVIFLFLGLLGVSLYGTTRVRDGLDLTDIVPRETREYDFIAAQFKYFSFYNMYIVTQKADYPNIQHLLYDLHRSFSNVKYVMLEENKQLPKMWLHYFRDWLQGLQDAFDSDWETGKIMPNNYKNGSDDGVLAYKLLVQTGSRDKPIDISQLTKQRLVDADGIINPSAFYIYLTAWVSNDPVAYAASQANIRPHRPEWVHDKADYMPETRLRIPAAEPIEYAQFPFYLNGLRDTSDFVEAIEKVRTICSNYTSLGLSSYPNGYPFLFWEQYIGLRHWLLLFISVVLACTFLVCAVFLLNPWTAGIIVMVLALMTVELFGMMGLIGIKLSAVPVVILIASVGIGVEFTVHVALAFLTAIGDKNRRAVLALEHMFAPVLDGAVSTLLGVLMLAGSEFDFIVRYFFAVLAILTILGVLNGLVLLPVLLSFFGPYPEVSPANGLNRLPTPSPEPPPSVVRFAMPPGHTHSGSDSSDSEYSSQTTVSGLSEELRHYEAQQGAGGPAHQVIVEATENPVFAHSTVVHPESRHHPPSNPRQQPHLDSGSLPPGRQGQQPRRDPPREGLWPPPYRPRRDAFEISTEGHSGPSNRARWGPRGARSHNPRNPASTAMGSSVPGYCQPITTVTASASVTVAVHPPPVPGPGRNPRGGLCPGYPETDHGLFEDPHVPFHVRCERRDSKVEVIELQDVECEERPRGSSSN

Target ORF information:

RefSeq Version	NM_001083606.2
Organism	Homo sapiens(human)
Definition	Homo sapiens patched 1 (PTCH1), transcript variant 1d, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001083606.2

ORF Insert Sequence:

ATGTTTAATC CTCAACTCAT GATACAGACC CCTAAAGAAG AAGGTGCTAA TGTCCTGACC 
ACAGAAGCGC TCCTACAACA CCTGGACTCG GCACTCCAGG CCAGCCGTGT CCATGTATAC 
ATGTACAACA GGCAGTGGAA ATTGGAACAT TTGTGTTACA AATCAGGAGA GCTTATCACA 
GAAACAGGTT ACATGGATCA GATAATAGAA TATCTTTACC CTTGTTTGAT TATTACACCT 
TTGGACTGCT TCTGGGAAGG GGCGAAATTA CAGTCTGGGA CAGCATACCT CCTAGGTAAA 
CCTCCTTTGC GGTGGACAAA CTTCGACCCT TTGGAATTCC TGGAAGAGTT AAAGAAAATA 
AACTATCAAG TGGACAGCTG GGAGGAAATG CTGAATAAGG CTGAGGTTGG TCATGGTTAC 
ATGGACCGCC CCTGCCTCAA TCCGGCCGAT CCAGACTGCC CCGCCACAGC CCCCAACAAA 
AATTCAACCA AACCTCTTGA TATGGCCCTT GTTTTGAATG GTGGATGTCA TGGCTTATCC 
AGAAAGTATA TGCACTGGCA GGAGGAGTTG ATTGTGGGTG GCACAGTCAA GAACAGCACT 
GGAAAACTCG TCAGCGCCCA TGCCCTGCAG ACCATGTTCC AGTTAATGAC TCCCAAGCAA 
ATGTACGAGC ACTTCAAGGG GTACGAGTAT GTCTCACACA TCAACTGGAA CGAGGACAAA 
GCGGCAGCCA TCCTGGAGGC CTGGCAGAGG ACATATGTGG AGGTGGTTCA TCAGAGTGTC 
GCACAGAACT CCACTCAAAA GGTGCTTTCC TTCACCACCA CGACCCTGGA CGACATCCTG 
AAATCCTTCT CTGACGTCAG TGTCATCCGC GTGGCCAGCG GCTACTTACT CATGCTCGCC 
TATGCCTGTC TAACCATGCT GCGCTGGGAC TGCTCCAAGT CCCAGGGTGC CGTGGGGCTG 
GCTGGCGTCC TGCTGGTTGC ACTGTCAGTG GCTGCAGGAC TGGGCCTGTG CTCATTGATC 
GGAATTTCCT TTAACGCTGC AACAACTCAG GTTTTGCCAT TTCTCGCTCT TGGTGTTGGT 
GTGGATGATG TTTTTCTTCT GGCCCACGCC TTCAGTGAAA CAGGACAGAA TAAAAGAATC 
CCTTTTGAGG ACAGGACCGG GGAGTGCCTG AAGCGCACAG GAGCCAGCGT GGCCCTCACG 
TCCATCAGCA ATGTCACAGC CTTCTTCATG GCCGCGTTAA TCCCAATTCC CGCTCTGCGG 
GCGTTCTCCC TCCAGGCAGC GGTAGTAGTG GTGTTCAATT TTGCCATGGT TCTGCTCATT 
TTTCCTGCAA TTCTCAGCAT GGATTTATAT CGACGCGAGG ACAGGAGACT GGATATTTTC 
TGCTGTTTTA CAAGCCCCTG CGTCAGCAGA GTGATTCAGG TTGAACCTCA GGCCTACACC 
GACACACACG ACAATACCCG CTACAGCCCC CCACCTCCCT ACAGCAGCCA CAGCTTTGCC 
CATGAAACGC AGATTACCAT GCAGTCCACT GTCCAGCTCC GCACGGAGTA CGACCCCCAC 
ACGCACGTGT ACTACACCAC CGCTGAGCCG CGCTCCGAGA TCTCTGTGCA GCCCGTCACC 
GTGACACAGG ACACCCTCAG CTGCCAGAGC CCAGAGAGCA CCAGCTCCAC AAGGGACCTG 
CTCTCCCAGT TCTCCGACTC CAGCCTCCAC TGCCTCGAGC CCCCCTGTAC GAAGTGGACA 
CTCTCATCTT TTGCTGAGAA GCACTATGCT CCTTTCCTCT TGAAACCAAA AGCCAAGGTA 
GTGGTGATCT TCCTTTTTCT GGGCTTGCTG GGGGTCAGCC TTTATGGCAC CACCCGAGTG 
AGAGACGGGC TGGACCTTAC GGACATTGTA CCTCGGGAAA CCAGAGAATA TGACTTTATT 
GCTGCACAAT TCAAATACTT TTCTTTCTAC AACATGTATA TAGTCACCCA GAAAGCAGAC 
TACCCGAATA TCCAGCACTT ACTTTACGAC CTACACAGGA GTTTCAGTAA CGTGAAGTAT 
GTCATGTTGG AAGAAAACAA ACAGCTTCCC AAAATGTGGC TGCACTACTT CAGAGACTGG 
CTTCAGGGAC TTCAGGATGC ATTTGACAGT GACTGGGAAA CCGGGAAAAT CATGCCAAAC 
AATTACAAGA ATGGATCAGA CGATGGAGTC CTTGCCTACA AACTCCTGGT GCAAACCGGC 
AGCCGCGATA AGCCCATCGA CATCAGCCAG TTGACTAAAC AGCGTCTGGT GGATGCAGAT 
GGCATCATTA ATCCCAGCGC TTTCTACATC TACCTGACGG CTTGGGTCAG CAACGACCCC 
GTCGCGTATG CTGCCTCCCA GGCCAACATC CGGCCACACC GACCAGAATG GGTCCACGAC 
AAAGCCGACT ACATGCCTGA AACAAGGCTG AGAATCCCGG CAGCAGAGCC CATCGAGTAT 
GCCCAGTTCC CTTTCTACCT CAACGGCTTG CGGGACACCT CAGACTTTGT GGAGGCAATT 
GAAAAAGTAA GGACCATCTG CAGCAACTAT ACGAGCCTGG GGCTGTCCAG TTACCCCAAC 
GGCTACCCCT TCCTCTTCTG GGAGCAGTAC ATCGGCCTCC GCCACTGGCT GCTGCTGTTC 
ATCAGCGTGG TGTTGGCCTG CACATTCCTC GTGTGCGCTG TCTTCCTTCT GAACCCCTGG 
ACGGCCGGGA TCATTGTGAT GGTCCTGGCG CTGATGACGG TCGAGCTGTT CGGCATGATG 
GGCCTCATCG GAATCAAGCT CAGTGCCGTG CCCGTGGTCA TCCTGATCGC TTCTGTTGGC 
ATAGGAGTGG AGTTCACCGT TCACGTTGCT TTGGCCTTTC TGACGGCCAT CGGCGACAAG 
AACCGCAGGG CTGTGCTTGC CCTGGAGCAC ATGTTTGCAC CCGTCCTGGA TGGCGCCGTG 
TCCACTCTGC TGGGAGTGCT GATGCTGGCG GGATCTGAGT TCGACTTCAT TGTCAGGTAT 
TTCTTTGCTG TGCTGGCGAT CCTCACCATC CTCGGCGTTC TCAATGGGCT GGTTTTGCTT 
CCCGTGCTTT TGTCTTTCTT TGGACCATAT CCTGAGGTGT CTCCAGCCAA CGGCTTGAAC 
CGCCTGCCCA CACCCTCCCC TGAGCCACCC CCCAGCGTGG TCCGCTTCGC CATGCCGCCC 
GGCCACACGC ACAGCGGGTC TGATTCCTCC GACTCGGAGT ATAGTTCCCA GACGACAGTG 
TCAGGCCTCA GCGAGGAGCT TCGGCACTAC GAGGCCCAGC AGGGCGCGGG AGGCCCTGCC 
CACCAAGTGA TCGTGGAAGC CACAGAAAAC CCCGTCTTCG CCCACTCCAC TGTGGTCCAT 
CCCGAATCCA GGCATCACCC ACCCTCGAAC CCGAGACAGC AGCCCCACCT GGACTCAGGG 
TCCCTGCCTC CCGGACGGCA AGGCCAGCAG CCCCGCAGGG ACCCCCCCAG AGAAGGCTTG 
TGGCCACCCC CCTACAGACC GCGCAGAGAC GCTTTTGAAA TTTCTACTGA AGGGCATTCT 
GGCCCTAGCA ATAGGGCCCG CTGGGGCCCT CGCGGGGCCC GTTCTCACAA CCCTCGGAAC 
CCAGCGTCCA CTGCCATGGG CAGCTCCGTG CCCGGCTACT GCCAGCCCAT CACCACTGTG 
ACGGCTTCTG CCTCCGTGAC TGTCGCCGTG CACCCGCCGC CTGTCCCTGG GCCTGGGCGG 
AACCCCCGAG GGGGACTCTG CCCAGGCTAC CCTGAGACTG ACCACGGCCT GTTTGAGGAC 
CCCCACGTGC CTTTCCACGT CCGGTGTGAG AGGAGGGATT CGAAGGTGGA AGTCATTGAG 
CTGCAGGACG TGGAATGCGA GGAGAGGCCC CGGGGAAGCA GCTCCAACTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PTCH1 ( NM_001083606.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001077075.1 Homo sapiens patched 1 (PTCH1), transcript variant 1d, mRNA.

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CloneID	OHu17265
Clone ID Related Accession (Same CDS sequence)	XM_005252102.2 , XM_011518872.1 , NM_001083605.1 , NM_001083604.2 , NM_001083606.2 , NM_001083606.1 , XM_011518871.1 , XM_011518869.1 , NM_001083604.1 , XM_011518870.1 , NM_001083607.1 , NM_001083606.3 , NM_001083605.2 , NM_001083607.2
Accession Version	NM_001083606.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3891bp) Protein sequence SNP
Vector	pcDNA3.1⁺/C-(K)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-04-24
Organism	Homo sapiens(Human)
Product	protein patched homolog 1 isoform S
Comment	REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from U43148.1, AI358880.1, AB209495.1, BX117041.1 and CR744004.1. Transcript Variant: This variant (1d) differs in the 5' UTR and has multiple differences in the 5' coding region, compared to variant 1b. These differences cause translation initiation from a downstream ATG and an isoform (S) with a shorter N-terminus, compared to isoform L. Variants 1c, 1c', 1d, and 1e encode the same isoform (S). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: U43148.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGTTTAATC CTCAACTCAT GATACAGACC CCTAAAGAAG AAGGTGCTAA TGTCCTGACC 
ACAGAAGCGC TCCTACAACA CCTGGACTCG GCACTCCAGG CCAGCCGTGT CCATGTATAC 
ATGTACAACA GGCAGTGGAA ATTGGAACAT TTGTGTTACA AATCAGGAGA GCTTATCACA 
GAAACAGGTT ACATGGATCA GATAATAGAA TATCTTTACC CTTGTTTGAT TATTACACCT 
TTGGACTGCT TCTGGGAAGG GGCGAAATTA CAGTCTGGGA CAGCATACCT CCTAGGTAAA 
CCTCCTTTGC GGTGGACAAA CTTCGACCCT TTGGAATTCC TGGAAGAGTT AAAGAAAATA 
AACTATCAAG TGGACAGCTG GGAGGAAATG CTGAATAAGG CTGAGGTTGG TCATGGTTAC 
ATGGACCGCC CCTGCCTCAA TCCGGCCGAT CCAGACTGCC CCGCCACAGC CCCCAACAAA 
AATTCAACCA AACCTCTTGA TATGGCCCTT GTTTTGAATG GTGGATGTCA TGGCTTATCC 
AGAAAGTATA TGCACTGGCA GGAGGAGTTG ATTGTGGGTG GCACAGTCAA GAACAGCACT 
GGAAAACTCG TCAGCGCCCA TGCCCTGCAG ACCATGTTCC AGTTAATGAC TCCCAAGCAA 
ATGTACGAGC ACTTCAAGGG GTACGAGTAT GTCTCACACA TCAACTGGAA CGAGGACAAA 
GCGGCAGCCA TCCTGGAGGC CTGGCAGAGG ACATATGTGG AGGTGGTTCA TCAGAGTGTC 
GCACAGAACT CCACTCAAAA GGTGCTTTCC TTCACCACCA CGACCCTGGA CGACATCCTG 
AAATCCTTCT CTGACGTCAG TGTCATCCGC GTGGCCAGCG GCTACTTACT CATGCTCGCC 
TATGCCTGTC TAACCATGCT GCGCTGGGAC TGCTCCAAGT CCCAGGGTGC CGTGGGGCTG 
GCTGGCGTCC TGCTGGTTGC ACTGTCAGTG GCTGCAGGAC TGGGCCTGTG CTCATTGATC 
GGAATTTCCT TTAACGCTGC AACAACTCAG GTTTTGCCAT TTCTCGCTCT TGGTGTTGGT 
GTGGATGATG TTTTTCTTCT GGCCCACGCC TTCAGTGAAA CAGGACAGAA TAAAAGAATC 
CCTTTTGAGG ACAGGACCGG GGAGTGCCTG AAGCGCACAG GAGCCAGCGT GGCCCTCACG 
TCCATCAGCA ATGTCACAGC CTTCTTCATG GCCGCGTTAA TCCCAATTCC CGCTCTGCGG 
GCGTTCTCCC TCCAGGCAGC GGTAGTAGTG GTGTTCAATT TTGCCATGGT TCTGCTCATT 
TTTCCTGCAA TTCTCAGCAT GGATTTATAT CGACGCGAGG ACAGGAGACT GGATATTTTC 
TGCTGTTTTA CAAGCCCCTG CGTCAGCAGA GTGATTCAGG TTGAACCTCA GGCCTACACC 
GACACACACG ACAATACCCG CTACAGCCCC CCACCTCCCT ACAGCAGCCA CAGCTTTGCC 
CATGAAACGC AGATTACCAT GCAGTCCACT GTCCAGCTCC GCACGGAGTA CGACCCCCAC 
ACGCACGTGT ACTACACCAC CGCTGAGCCG CGCTCCGAGA TCTCTGTGCA GCCCGTCACC 
GTGACACAGG ACACCCTCAG CTGCCAGAGC CCAGAGAGCA CCAGCTCCAC AAGGGACCTG 
CTCTCCCAGT TCTCCGACTC CAGCCTCCAC TGCCTCGAGC CCCCCTGTAC GAAGTGGACA 
CTCTCATCTT TTGCTGAGAA GCACTATGCT CCTTTCCTCT TGAAACCAAA AGCCAAGGTA 
GTGGTGATCT TCCTTTTTCT GGGCTTGCTG GGGGTCAGCC TTTATGGCAC CACCCGAGTG 
AGAGACGGGC TGGACCTTAC GGACATTGTA CCTCGGGAAA CCAGAGAATA TGACTTTATT 
GCTGCACAAT TCAAATACTT TTCTTTCTAC AACATGTATA TAGTCACCCA GAAAGCAGAC 
TACCCGAATA TCCAGCACTT ACTTTACGAC CTACACAGGA GTTTCAGTAA CGTGAAGTAT 
GTCATGTTGG AAGAAAACAA ACAGCTTCCC AAAATGTGGC TGCACTACTT CAGAGACTGG 
CTTCAGGGAC TTCAGGATGC ATTTGACAGT GACTGGGAAA CCGGGAAAAT CATGCCAAAC 
AATTACAAGA ATGGATCAGA CGATGGAGTC CTTGCCTACA AACTCCTGGT GCAAACCGGC 
AGCCGCGATA AGCCCATCGA CATCAGCCAG TTGACTAAAC AGCGTCTGGT GGATGCAGAT 
GGCATCATTA ATCCCAGCGC TTTCTACATC TACCTGACGG CTTGGGTCAG CAACGACCCC 
GTCGCGTATG CTGCCTCCCA GGCCAACATC CGGCCACACC GACCAGAATG GGTCCACGAC 
AAAGCCGACT ACATGCCTGA AACAAGGCTG AGAATCCCGG CAGCAGAGCC CATCGAGTAT 
GCCCAGTTCC CTTTCTACCT CAACGGCTTG CGGGACACCT CAGACTTTGT GGAGGCAATT 
GAAAAAGTAA GGACCATCTG CAGCAACTAT ACGAGCCTGG GGCTGTCCAG TTACCCCAAC 
GGCTACCCCT TCCTCTTCTG GGAGCAGTAC ATCGGCCTCC GCCACTGGCT GCTGCTGTTC 
ATCAGCGTGG TGTTGGCCTG CACATTCCTC GTGTGCGCTG TCTTCCTTCT GAACCCCTGG 
ACGGCCGGGA TCATTGTGAT GGTCCTGGCG CTGATGACGG TCGAGCTGTT CGGCATGATG 
GGCCTCATCG GAATCAAGCT CAGTGCCGTG CCCGTGGTCA TCCTGATCGC TTCTGTTGGC 
ATAGGAGTGG AGTTCACCGT TCACGTTGCT TTGGCCTTTC TGACGGCCAT CGGCGACAAG 
AACCGCAGGG CTGTGCTTGC CCTGGAGCAC ATGTTTGCAC CCGTCCTGGA TGGCGCCGTG 
TCCACTCTGC TGGGAGTGCT GATGCTGGCG GGATCTGAGT TCGACTTCAT TGTCAGGTAT 
TTCTTTGCTG TGCTGGCGAT CCTCACCATC CTCGGCGTTC TCAATGGGCT GGTTTTGCTT 
CCCGTGCTTT TGTCTTTCTT TGGACCATAT CCTGAGGTGT CTCCAGCCAA CGGCTTGAAC 
CGCCTGCCCA CACCCTCCCC TGAGCCACCC CCCAGCGTGG TCCGCTTCGC CATGCCGCCC 
GGCCACACGC ACAGCGGGTC TGATTCCTCC GACTCGGAGT ATAGTTCCCA GACGACAGTG 
TCAGGCCTCA GCGAGGAGCT TCGGCACTAC GAGGCCCAGC AGGGCGCGGG AGGCCCTGCC 
CACCAAGTGA TCGTGGAAGC CACAGAAAAC CCCGTCTTCG CCCACTCCAC TGTGGTCCAT 
CCCGAATCCA GGCATCACCC ACCCTCGAAC CCGAGACAGC AGCCCCACCT GGACTCAGGG 
TCCCTGCCTC CCGGACGGCA AGGCCAGCAG CCCCGCAGGG ACCCCCCCAG AGAAGGCTTG 
TGGCCACCCC CCTACAGACC GCGCAGAGAC GCTTTTGAAA TTTCTACTGA AGGGCATTCT 
GGCCCTAGCA ATAGGGCCCG CTGGGGCCCT CGCGGGGCCC GTTCTCACAA CCCTCGGAAC 
CCAGCGTCCA CTGCCATGGG CAGCTCCGTG CCCGGCTACT GCCAGCCCAT CACCACTGTG 
ACGGCTTCTG CCTCCGTGAC TGTCGCCGTG CACCCGCCGC CTGTCCCTGG GCCTGGGCGG 
AACCCCCGAG GGGGACTCTG CCCAGGCTAC CCTGAGACTG ACCACGGCCT GTTTGAGGAC 
CCCCACGTGC CTTTCCACGT CCGGTGTGAG AGGAGGGATT CGAAGGTGGA AGTCATTGAG 
CTGCAGGACG TGGAATGCGA GGAGAGGCCC CGGGGAAGCA GCTCCAACTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001077075.1
CDS	442..4332
Translation	MFNPQLMIQTPKEEGANVLTTEALLQHLDSALQASRVHVYMYNRQWKLEHLCYKSGELITETGYMDQIIEYLYPCLIITPLDCFWEGAKLQSGTAYLLGKPPLRWTNFDPLEFLEELKKINYQVDSWEEMLNKAEVGHGYMDRPCLNPADPDCPATAPNKNSTKPLDMALVLNGGCHGLSRKYMHWQEELIVGGTVKNSTGKLVSAHALQTMFQLMTPKQMYEHFKGYEYVSHINWNEDKAAAILEAWQRTYVEVVHQSVAQNSTQKVLSFTTTTLDDILKSFSDVSVIRVASGYLLMLAYACLTMLRWDCSKSQGAVGLAGVLLVALSVAAGLGLCSLIGISFNAATTQVLPFLALGVGVDDVFLLAHAFSETGQNKRIPFEDRTGECLKRTGASVALTSISNVTAFFMAALIPIPALRAFSLQAAVVVVFNFAMVLLIFPAILSMDLYRREDRRLDIFCCFTSPCVSRVIQVEPQAYTDTHDNTRYSPPPPYSSHSFAHETQITMQSTVQLRTEYDPHTHVYYTTAEPRSEISVQPVTVTQDTLSCQSPESTSSTRDLLSQFSDSSLHCLEPPCTKWTLSSFAEKHYAPFLLKPKAKVVVIFLFLGLLGVSLYGTTRVRDGLDLTDIVPRETREYDFIAAQFKYFSFYNMYIVTQKADYPNIQHLLYDLHRSFSNVKYVMLEENKQLPKMWLHYFRDWLQGLQDAFDSDWETGKIMPNNYKNGSDDGVLAYKLLVQTGSRDKPIDISQLTKQRLVDADGIINPSAFYIYLTAWVSNDPVAYAASQANIRPHRPEWVHDKADYMPETRLRIPAAEPIEYAQFPFYLNGLRDTSDFVEAIEKVRTICSNYTSLGLSSYPNGYPFLFWEQYIGLRHWLLLFISVVLACTFLVCAVFLLNPWTAGIIVMVLALMTVELFGMMGLIGIKLSAVPVVILIASVGIGVEFTVHVALAFLTAIGDKNRRAVLALEHMFAPVLDGAVSTLLGVLMLAGSEFDFIVRYFFAVLAILTILGVLNGLVLLPVLLSFFGPYPEVSPANGLNRLPTPSPEPPPSVVRFAMPPGHTHSGSDSSDSEYSSQTTVSGLSEELRHYEAQQGAGGPAHQVIVEATENPVFAHSTVVHPESRHHPPSNPRQQPHLDSGSLPPGRQGQQPRRDPPREGLWPPPYRPRRDAFEISTEGHSGPSNRARWGPRGARSHNPRNPASTAMGSSVPGYCQPITTVTASASVTVAVHPPPVPGPGRNPRGGLCPGYPETDHGLFEDPHVPFHVRCERRDSKVEVIELQDVECEERPRGSSSN

Target ORF information:

RefSeq Version	NM_001083606.1
Organism	Homo sapiens(Human)
Definition	Homo sapiens patched 1 (PTCH1), transcript variant 1d, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001083606.1

ORF Insert Sequence:

ATGTTTAATC CTCAACTCAT GATACAGACC CCTAAAGAAG AAGGTGCTAA TGTCCTGACC 
ACAGAAGCGC TCCTACAACA CCTGGACTCG GCACTCCAGG CCAGCCGTGT CCATGTATAC 
ATGTACAACA GGCAGTGGAA ATTGGAACAT TTGTGTTACA AATCAGGAGA GCTTATCACA 
GAAACAGGTT ACATGGATCA GATAATAGAA TATCTTTACC CTTGTTTGAT TATTACACCT 
TTGGACTGCT TCTGGGAAGG GGCGAAATTA CAGTCTGGGA CAGCATACCT CCTAGGTAAA 
CCTCCTTTGC GGTGGACAAA CTTCGACCCT TTGGAATTCC TGGAAGAGTT AAAGAAAATA 
AACTATCAAG TGGACAGCTG GGAGGAAATG CTGAATAAGG CTGAGGTTGG TCATGGTTAC 
ATGGACCGCC CCTGCCTCAA TCCGGCCGAT CCAGACTGCC CCGCCACAGC CCCCAACAAA 
AATTCAACCA AACCTCTTGA TATGGCCCTT GTTTTGAATG GTGGATGTCA TGGCTTATCC 
AGAAAGTATA TGCACTGGCA GGAGGAGTTG ATTGTGGGTG GCACAGTCAA GAACAGCACT 
GGAAAACTCG TCAGCGCCCA TGCCCTGCAG ACCATGTTCC AGTTAATGAC TCCCAAGCAA 
ATGTACGAGC ACTTCAAGGG GTACGAGTAT GTCTCACACA TCAACTGGAA CGAGGACAAA 
GCGGCAGCCA TCCTGGAGGC CTGGCAGAGG ACATATGTGG AGGTGGTTCA TCAGAGTGTC 
GCACAGAACT CCACTCAAAA GGTGCTTTCC TTCACCACCA CGACCCTGGA CGACATCCTG 
AAATCCTTCT CTGACGTCAG TGTCATCCGC GTGGCCAGCG GCTACTTACT CATGCTCGCC 
TATGCCTGTC TAACCATGCT GCGCTGGGAC TGCTCCAAGT CCCAGGGTGC CGTGGGGCTG 
GCTGGCGTCC TGCTGGTTGC ACTGTCAGTG GCTGCAGGAC TGGGCCTGTG CTCATTGATC 
GGAATTTCCT TTAACGCTGC AACAACTCAG GTTTTGCCAT TTCTCGCTCT TGGTGTTGGT 
GTGGATGATG TTTTTCTTCT GGCCCACGCC TTCAGTGAAA CAGGACAGAA TAAAAGAATC 
CCTTTTGAGG ACAGGACCGG GGAGTGCCTG AAGCGCACAG GAGCCAGCGT GGCCCTCACG 
TCCATCAGCA ATGTCACAGC CTTCTTCATG GCCGCGTTAA TCCCAATTCC CGCTCTGCGG 
GCGTTCTCCC TCCAGGCAGC GGTAGTAGTG GTGTTCAATT TTGCCATGGT TCTGCTCATT 
TTTCCTGCAA TTCTCAGCAT GGATTTATAT CGACGCGAGG ACAGGAGACT GGATATTTTC 
TGCTGTTTTA CAAGCCCCTG CGTCAGCAGA GTGATTCAGG TTGAACCTCA GGCCTACACC 
GACACACACG ACAATACCCG CTACAGCCCC CCACCTCCCT ACAGCAGCCA CAGCTTTGCC 
CATGAAACGC AGATTACCAT GCAGTCCACT GTCCAGCTCC GCACGGAGTA CGACCCCCAC 
ACGCACGTGT ACTACACCAC CGCTGAGCCG CGCTCCGAGA TCTCTGTGCA GCCCGTCACC 
GTGACACAGG ACACCCTCAG CTGCCAGAGC CCAGAGAGCA CCAGCTCCAC AAGGGACCTG 
CTCTCCCAGT TCTCCGACTC CAGCCTCCAC TGCCTCGAGC CCCCCTGTAC GAAGTGGACA 
CTCTCATCTT TTGCTGAGAA GCACTATGCT CCTTTCCTCT TGAAACCAAA AGCCAAGGTA 
GTGGTGATCT TCCTTTTTCT GGGCTTGCTG GGGGTCAGCC TTTATGGCAC CACCCGAGTG 
AGAGACGGGC TGGACCTTAC GGACATTGTA CCTCGGGAAA CCAGAGAATA TGACTTTATT 
GCTGCACAAT TCAAATACTT TTCTTTCTAC AACATGTATA TAGTCACCCA GAAAGCAGAC 
TACCCGAATA TCCAGCACTT ACTTTACGAC CTACACAGGA GTTTCAGTAA CGTGAAGTAT 
GTCATGTTGG AAGAAAACAA ACAGCTTCCC AAAATGTGGC TGCACTACTT CAGAGACTGG 
CTTCAGGGAC TTCAGGATGC ATTTGACAGT GACTGGGAAA CCGGGAAAAT CATGCCAAAC 
AATTACAAGA ATGGATCAGA CGATGGAGTC CTTGCCTACA AACTCCTGGT GCAAACCGGC 
AGCCGCGATA AGCCCATCGA CATCAGCCAG TTGACTAAAC AGCGTCTGGT GGATGCAGAT 
GGCATCATTA ATCCCAGCGC TTTCTACATC TACCTGACGG CTTGGGTCAG CAACGACCCC 
GTCGCGTATG CTGCCTCCCA GGCCAACATC CGGCCACACC GACCAGAATG GGTCCACGAC 
AAAGCCGACT ACATGCCTGA AACAAGGCTG AGAATCCCGG CAGCAGAGCC CATCGAGTAT 
GCCCAGTTCC CTTTCTACCT CAACGGCTTG CGGGACACCT CAGACTTTGT GGAGGCAATT 
GAAAAAGTAA GGACCATCTG CAGCAACTAT ACGAGCCTGG GGCTGTCCAG TTACCCCAAC 
GGCTACCCCT TCCTCTTCTG GGAGCAGTAC ATCGGCCTCC GCCACTGGCT GCTGCTGTTC 
ATCAGCGTGG TGTTGGCCTG CACATTCCTC GTGTGCGCTG TCTTCCTTCT GAACCCCTGG 
ACGGCCGGGA TCATTGTGAT GGTCCTGGCG CTGATGACGG TCGAGCTGTT CGGCATGATG 
GGCCTCATCG GAATCAAGCT CAGTGCCGTG CCCGTGGTCA TCCTGATCGC TTCTGTTGGC 
ATAGGAGTGG AGTTCACCGT TCACGTTGCT TTGGCCTTTC TGACGGCCAT CGGCGACAAG 
AACCGCAGGG CTGTGCTTGC CCTGGAGCAC ATGTTTGCAC CCGTCCTGGA TGGCGCCGTG 
TCCACTCTGC TGGGAGTGCT GATGCTGGCG GGATCTGAGT TCGACTTCAT TGTCAGGTAT 
TTCTTTGCTG TGCTGGCGAT CCTCACCATC CTCGGCGTTC TCAATGGGCT GGTTTTGCTT 
CCCGTGCTTT TGTCTTTCTT TGGACCATAT CCTGAGGTGT CTCCAGCCAA CGGCTTGAAC 
CGCCTGCCCA CACCCTCCCC TGAGCCACCC CCCAGCGTGG TCCGCTTCGC CATGCCGCCC 
GGCCACACGC ACAGCGGGTC TGATTCCTCC GACTCGGAGT ATAGTTCCCA GACGACAGTG 
TCAGGCCTCA GCGAGGAGCT TCGGCACTAC GAGGCCCAGC AGGGCGCGGG AGGCCCTGCC 
CACCAAGTGA TCGTGGAAGC CACAGAAAAC CCCGTCTTCG CCCACTCCAC TGTGGTCCAT 
CCCGAATCCA GGCATCACCC ACCCTCGAAC CCGAGACAGC AGCCCCACCT GGACTCAGGG 
TCCCTGCCTC CCGGACGGCA AGGCCAGCAG CCCCGCAGGG ACCCCCCCAG AGAAGGCTTG 
TGGCCACCCC CCTACAGACC GCGCAGAGAC GCTTTTGAAA TTTCTACTGA AGGGCATTCT 
GGCCCTAGCA ATAGGGCCCG CTGGGGCCCT CGCGGGGCCC GTTCTCACAA CCCTCGGAAC 
CCAGCGTCCA CTGCCATGGG CAGCTCCGTG CCCGGCTACT GCCAGCCCAT CACCACTGTG 
ACGGCTTCTG CCTCCGTGAC TGTCGCCGTG CACCCGCCGC CTGTCCCTGG GCCTGGGCGG 
AACCCCCGAG GGGGACTCTG CCCAGGCTAC CCTGAGACTG ACCACGGCCT GTTTGAGGAC 
CCCCACGTGC CTTTCCACGT CCGGTGTGAG AGGAGGGATT CGAAGGTGGA AGTCATTGAG 
CTGCAGGACG TGGAATGCGA GGAGAGGCCC CGGGGAAGCA GCTCCAACTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PTCH1 ( NM_001083604.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001077073.1 Homo sapiens patched 1 (PTCH1), transcript variant 1c, mRNA.

Price & Availability↑

CloneID	OHu17265
Clone ID Related Accession (Same CDS sequence)	XM_005252102.2 , XM_011518872.1 , NM_001083605.1 , NM_001083604.2 , NM_001083606.2 , NM_001083606.1 , XM_011518871.1 , XM_011518869.1 , NM_001083604.1 , XM_011518870.1 , NM_001083607.1 , NM_001083606.3 , NM_001083605.2 , NM_001083607.2
Accession Version	NM_001083604.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3891bp) Protein sequence SNP
Vector	pcDNA3.1⁺/C-(K)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-04-24
Organism	Homo sapiens(Human)
Product	protein patched homolog 1 isoform S
Comment	REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AB189438.1, U59464.1, AB209495.1, BX117041.1 and CR744004.1. Transcript Variant: This variant (1c) differs in the 5' UTR and has multiple differences in the 5' coding region, compared to variant 1b. These differences cause translation initiation from a downstream ATG and an isoform (S) with a shorter N-terminus, compared to isoform L. Variants 1c, 1c', 1d, and 1e encode the same isoform (S). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## CDS exon combination :: U43148.1 [ECO:0000331] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGTTTAATC CTCAACTCAT GATACAGACC CCTAAAGAAG AAGGTGCTAA TGTCCTGACC 
ACAGAAGCGC TCCTACAACA CCTGGACTCG GCACTCCAGG CCAGCCGTGT CCATGTATAC 
ATGTACAACA GGCAGTGGAA ATTGGAACAT TTGTGTTACA AATCAGGAGA GCTTATCACA 
GAAACAGGTT ACATGGATCA GATAATAGAA TATCTTTACC CTTGTTTGAT TATTACACCT 
TTGGACTGCT TCTGGGAAGG GGCGAAATTA CAGTCTGGGA CAGCATACCT CCTAGGTAAA 
CCTCCTTTGC GGTGGACAAA CTTCGACCCT TTGGAATTCC TGGAAGAGTT AAAGAAAATA 
AACTATCAAG TGGACAGCTG GGAGGAAATG CTGAATAAGG CTGAGGTTGG TCATGGTTAC 
ATGGACCGCC CCTGCCTCAA TCCGGCCGAT CCAGACTGCC CCGCCACAGC CCCCAACAAA 
AATTCAACCA AACCTCTTGA TATGGCCCTT GTTTTGAATG GTGGATGTCA TGGCTTATCC 
AGAAAGTATA TGCACTGGCA GGAGGAGTTG ATTGTGGGTG GCACAGTCAA GAACAGCACT 
GGAAAACTCG TCAGCGCCCA TGCCCTGCAG ACCATGTTCC AGTTAATGAC TCCCAAGCAA 
ATGTACGAGC ACTTCAAGGG GTACGAGTAT GTCTCACACA TCAACTGGAA CGAGGACAAA 
GCGGCAGCCA TCCTGGAGGC CTGGCAGAGG ACATATGTGG AGGTGGTTCA TCAGAGTGTC 
GCACAGAACT CCACTCAAAA GGTGCTTTCC TTCACCACCA CGACCCTGGA CGACATCCTG 
AAATCCTTCT CTGACGTCAG TGTCATCCGC GTGGCCAGCG GCTACTTACT CATGCTCGCC 
TATGCCTGTC TAACCATGCT GCGCTGGGAC TGCTCCAAGT CCCAGGGTGC CGTGGGGCTG 
GCTGGCGTCC TGCTGGTTGC ACTGTCAGTG GCTGCAGGAC TGGGCCTGTG CTCATTGATC 
GGAATTTCCT TTAACGCTGC AACAACTCAG GTTTTGCCAT TTCTCGCTCT TGGTGTTGGT 
GTGGATGATG TTTTTCTTCT GGCCCACGCC TTCAGTGAAA CAGGACAGAA TAAAAGAATC 
CCTTTTGAGG ACAGGACCGG GGAGTGCCTG AAGCGCACAG GAGCCAGCGT GGCCCTCACG 
TCCATCAGCA ATGTCACAGC CTTCTTCATG GCCGCGTTAA TCCCAATTCC CGCTCTGCGG 
GCGTTCTCCC TCCAGGCAGC GGTAGTAGTG GTGTTCAATT TTGCCATGGT TCTGCTCATT 
TTTCCTGCAA TTCTCAGCAT GGATTTATAT CGACGCGAGG ACAGGAGACT GGATATTTTC 
TGCTGTTTTA CAAGCCCCTG CGTCAGCAGA GTGATTCAGG TTGAACCTCA GGCCTACACC 
GACACACACG ACAATACCCG CTACAGCCCC CCACCTCCCT ACAGCAGCCA CAGCTTTGCC 
CATGAAACGC AGATTACCAT GCAGTCCACT GTCCAGCTCC GCACGGAGTA CGACCCCCAC 
ACGCACGTGT ACTACACCAC CGCTGAGCCG CGCTCCGAGA TCTCTGTGCA GCCCGTCACC 
GTGACACAGG ACACCCTCAG CTGCCAGAGC CCAGAGAGCA CCAGCTCCAC AAGGGACCTG 
CTCTCCCAGT TCTCCGACTC CAGCCTCCAC TGCCTCGAGC CCCCCTGTAC GAAGTGGACA 
CTCTCATCTT TTGCTGAGAA GCACTATGCT CCTTTCCTCT TGAAACCAAA AGCCAAGGTA 
GTGGTGATCT TCCTTTTTCT GGGCTTGCTG GGGGTCAGCC TTTATGGCAC CACCCGAGTG 
AGAGACGGGC TGGACCTTAC GGACATTGTA CCTCGGGAAA CCAGAGAATA TGACTTTATT 
GCTGCACAAT TCAAATACTT TTCTTTCTAC AACATGTATA TAGTCACCCA GAAAGCAGAC 
TACCCGAATA TCCAGCACTT ACTTTACGAC CTACACAGGA GTTTCAGTAA CGTGAAGTAT 
GTCATGTTGG AAGAAAACAA ACAGCTTCCC AAAATGTGGC TGCACTACTT CAGAGACTGG 
CTTCAGGGAC TTCAGGATGC ATTTGACAGT GACTGGGAAA CCGGGAAAAT CATGCCAAAC 
AATTACAAGA ATGGATCAGA CGATGGAGTC CTTGCCTACA AACTCCTGGT GCAAACCGGC 
AGCCGCGATA AGCCCATCGA CATCAGCCAG TTGACTAAAC AGCGTCTGGT GGATGCAGAT 
GGCATCATTA ATCCCAGCGC TTTCTACATC TACCTGACGG CTTGGGTCAG CAACGACCCC 
GTCGCGTATG CTGCCTCCCA GGCCAACATC CGGCCACACC GACCAGAATG GGTCCACGAC 
AAAGCCGACT ACATGCCTGA AACAAGGCTG AGAATCCCGG CAGCAGAGCC CATCGAGTAT 
GCCCAGTTCC CTTTCTACCT CAACGGCTTG CGGGACACCT CAGACTTTGT GGAGGCAATT 
GAAAAAGTAA GGACCATCTG CAGCAACTAT ACGAGCCTGG GGCTGTCCAG TTACCCCAAC 
GGCTACCCCT TCCTCTTCTG GGAGCAGTAC ATCGGCCTCC GCCACTGGCT GCTGCTGTTC 
ATCAGCGTGG TGTTGGCCTG CACATTCCTC GTGTGCGCTG TCTTCCTTCT GAACCCCTGG 
ACGGCCGGGA TCATTGTGAT GGTCCTGGCG CTGATGACGG TCGAGCTGTT CGGCATGATG 
GGCCTCATCG GAATCAAGCT CAGTGCCGTG CCCGTGGTCA TCCTGATCGC TTCTGTTGGC 
ATAGGAGTGG AGTTCACCGT TCACGTTGCT TTGGCCTTTC TGACGGCCAT CGGCGACAAG 
AACCGCAGGG CTGTGCTTGC CCTGGAGCAC ATGTTTGCAC CCGTCCTGGA TGGCGCCGTG 
TCCACTCTGC TGGGAGTGCT GATGCTGGCG GGATCTGAGT TCGACTTCAT TGTCAGGTAT 
TTCTTTGCTG TGCTGGCGAT CCTCACCATC CTCGGCGTTC TCAATGGGCT GGTTTTGCTT 
CCCGTGCTTT TGTCTTTCTT TGGACCATAT CCTGAGGTGT CTCCAGCCAA CGGCTTGAAC 
CGCCTGCCCA CACCCTCCCC TGAGCCACCC CCCAGCGTGG TCCGCTTCGC CATGCCGCCC 
GGCCACACGC ACAGCGGGTC TGATTCCTCC GACTCGGAGT ATAGTTCCCA GACGACAGTG 
TCAGGCCTCA GCGAGGAGCT TCGGCACTAC GAGGCCCAGC AGGGCGCGGG AGGCCCTGCC 
CACCAAGTGA TCGTGGAAGC CACAGAAAAC CCCGTCTTCG CCCACTCCAC TGTGGTCCAT 
CCCGAATCCA GGCATCACCC ACCCTCGAAC CCGAGACAGC AGCCCCACCT GGACTCAGGG 
TCCCTGCCTC CCGGACGGCA AGGCCAGCAG CCCCGCAGGG ACCCCCCCAG AGAAGGCTTG 
TGGCCACCCC CCTACAGACC GCGCAGAGAC GCTTTTGAAA TTTCTACTGA AGGGCATTCT 
GGCCCTAGCA ATAGGGCCCG CTGGGGCCCT CGCGGGGCCC GTTCTCACAA CCCTCGGAAC 
CCAGCGTCCA CTGCCATGGG CAGCTCCGTG CCCGGCTACT GCCAGCCCAT CACCACTGTG 
ACGGCTTCTG CCTCCGTGAC TGTCGCCGTG CACCCGCCGC CTGTCCCTGG GCCTGGGCGG 
AACCCCCGAG GGGGACTCTG CCCAGGCTAC CCTGAGACTG ACCACGGCCT GTTTGAGGAC 
CCCCACGTGC CTTTCCACGT CCGGTGTGAG AGGAGGGATT CGAAGGTGGA AGTCATTGAG 
CTGCAGGACG TGGAATGCGA GGAGAGGCCC CGGGGAAGCA GCTCCAACTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001077073.1
CDS	360..4250
Translation	MFNPQLMIQTPKEEGANVLTTEALLQHLDSALQASRVHVYMYNRQWKLEHLCYKSGELITETGYMDQIIEYLYPCLIITPLDCFWEGAKLQSGTAYLLGKPPLRWTNFDPLEFLEELKKINYQVDSWEEMLNKAEVGHGYMDRPCLNPADPDCPATAPNKNSTKPLDMALVLNGGCHGLSRKYMHWQEELIVGGTVKNSTGKLVSAHALQTMFQLMTPKQMYEHFKGYEYVSHINWNEDKAAAILEAWQRTYVEVVHQSVAQNSTQKVLSFTTTTLDDILKSFSDVSVIRVASGYLLMLAYACLTMLRWDCSKSQGAVGLAGVLLVALSVAAGLGLCSLIGISFNAATTQVLPFLALGVGVDDVFLLAHAFSETGQNKRIPFEDRTGECLKRTGASVALTSISNVTAFFMAALIPIPALRAFSLQAAVVVVFNFAMVLLIFPAILSMDLYRREDRRLDIFCCFTSPCVSRVIQVEPQAYTDTHDNTRYSPPPPYSSHSFAHETQITMQSTVQLRTEYDPHTHVYYTTAEPRSEISVQPVTVTQDTLSCQSPESTSSTRDLLSQFSDSSLHCLEPPCTKWTLSSFAEKHYAPFLLKPKAKVVVIFLFLGLLGVSLYGTTRVRDGLDLTDIVPRETREYDFIAAQFKYFSFYNMYIVTQKADYPNIQHLLYDLHRSFSNVKYVMLEENKQLPKMWLHYFRDWLQGLQDAFDSDWETGKIMPNNYKNGSDDGVLAYKLLVQTGSRDKPIDISQLTKQRLVDADGIINPSAFYIYLTAWVSNDPVAYAASQANIRPHRPEWVHDKADYMPETRLRIPAAEPIEYAQFPFYLNGLRDTSDFVEAIEKVRTICSNYTSLGLSSYPNGYPFLFWEQYIGLRHWLLLFISVVLACTFLVCAVFLLNPWTAGIIVMVLALMTVELFGMMGLIGIKLSAVPVVILIASVGIGVEFTVHVALAFLTAIGDKNRRAVLALEHMFAPVLDGAVSTLLGVLMLAGSEFDFIVRYFFAVLAILTILGVLNGLVLLPVLLSFFGPYPEVSPANGLNRLPTPSPEPPPSVVRFAMPPGHTHSGSDSSDSEYSSQTTVSGLSEELRHYEAQQGAGGPAHQVIVEATENPVFAHSTVVHPESRHHPPSNPRQQPHLDSGSLPPGRQGQQPRRDPPREGLWPPPYRPRRDAFEISTEGHSGPSNRARWGPRGARSHNPRNPASTAMGSSVPGYCQPITTVTASASVTVAVHPPPVPGPGRNPRGGLCPGYPETDHGLFEDPHVPFHVRCERRDSKVEVIELQDVECEERPRGSSSN

Target ORF information:

RefSeq Version	NM_001083604.1
Organism	Homo sapiens(Human)
Definition	Homo sapiens patched 1 (PTCH1), transcript variant 1c, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001083604.1

ORF Insert Sequence:

ATGTTTAATC CTCAACTCAT GATACAGACC CCTAAAGAAG AAGGTGCTAA TGTCCTGACC 
ACAGAAGCGC TCCTACAACA CCTGGACTCG GCACTCCAGG CCAGCCGTGT CCATGTATAC 
ATGTACAACA GGCAGTGGAA ATTGGAACAT TTGTGTTACA AATCAGGAGA GCTTATCACA 
GAAACAGGTT ACATGGATCA GATAATAGAA TATCTTTACC CTTGTTTGAT TATTACACCT 
TTGGACTGCT TCTGGGAAGG GGCGAAATTA CAGTCTGGGA CAGCATACCT CCTAGGTAAA 
CCTCCTTTGC GGTGGACAAA CTTCGACCCT TTGGAATTCC TGGAAGAGTT AAAGAAAATA 
AACTATCAAG TGGACAGCTG GGAGGAAATG CTGAATAAGG CTGAGGTTGG TCATGGTTAC 
ATGGACCGCC CCTGCCTCAA TCCGGCCGAT CCAGACTGCC CCGCCACAGC CCCCAACAAA 
AATTCAACCA AACCTCTTGA TATGGCCCTT GTTTTGAATG GTGGATGTCA TGGCTTATCC 
AGAAAGTATA TGCACTGGCA GGAGGAGTTG ATTGTGGGTG GCACAGTCAA GAACAGCACT 
GGAAAACTCG TCAGCGCCCA TGCCCTGCAG ACCATGTTCC AGTTAATGAC TCCCAAGCAA 
ATGTACGAGC ACTTCAAGGG GTACGAGTAT GTCTCACACA TCAACTGGAA CGAGGACAAA 
GCGGCAGCCA TCCTGGAGGC CTGGCAGAGG ACATATGTGG AGGTGGTTCA TCAGAGTGTC 
GCACAGAACT CCACTCAAAA GGTGCTTTCC TTCACCACCA CGACCCTGGA CGACATCCTG 
AAATCCTTCT CTGACGTCAG TGTCATCCGC GTGGCCAGCG GCTACTTACT CATGCTCGCC 
TATGCCTGTC TAACCATGCT GCGCTGGGAC TGCTCCAAGT CCCAGGGTGC CGTGGGGCTG 
GCTGGCGTCC TGCTGGTTGC ACTGTCAGTG GCTGCAGGAC TGGGCCTGTG CTCATTGATC 
GGAATTTCCT TTAACGCTGC AACAACTCAG GTTTTGCCAT TTCTCGCTCT TGGTGTTGGT 
GTGGATGATG TTTTTCTTCT GGCCCACGCC TTCAGTGAAA CAGGACAGAA TAAAAGAATC 
CCTTTTGAGG ACAGGACCGG GGAGTGCCTG AAGCGCACAG GAGCCAGCGT GGCCCTCACG 
TCCATCAGCA ATGTCACAGC CTTCTTCATG GCCGCGTTAA TCCCAATTCC CGCTCTGCGG 
GCGTTCTCCC TCCAGGCAGC GGTAGTAGTG GTGTTCAATT TTGCCATGGT TCTGCTCATT 
TTTCCTGCAA TTCTCAGCAT GGATTTATAT CGACGCGAGG ACAGGAGACT GGATATTTTC 
TGCTGTTTTA CAAGCCCCTG CGTCAGCAGA GTGATTCAGG TTGAACCTCA GGCCTACACC 
GACACACACG ACAATACCCG CTACAGCCCC CCACCTCCCT ACAGCAGCCA CAGCTTTGCC 
CATGAAACGC AGATTACCAT GCAGTCCACT GTCCAGCTCC GCACGGAGTA CGACCCCCAC 
ACGCACGTGT ACTACACCAC CGCTGAGCCG CGCTCCGAGA TCTCTGTGCA GCCCGTCACC 
GTGACACAGG ACACCCTCAG CTGCCAGAGC CCAGAGAGCA CCAGCTCCAC AAGGGACCTG 
CTCTCCCAGT TCTCCGACTC CAGCCTCCAC TGCCTCGAGC CCCCCTGTAC GAAGTGGACA 
CTCTCATCTT TTGCTGAGAA GCACTATGCT CCTTTCCTCT TGAAACCAAA AGCCAAGGTA 
GTGGTGATCT TCCTTTTTCT GGGCTTGCTG GGGGTCAGCC TTTATGGCAC CACCCGAGTG 
AGAGACGGGC TGGACCTTAC GGACATTGTA CCTCGGGAAA CCAGAGAATA TGACTTTATT 
GCTGCACAAT TCAAATACTT TTCTTTCTAC AACATGTATA TAGTCACCCA GAAAGCAGAC 
TACCCGAATA TCCAGCACTT ACTTTACGAC CTACACAGGA GTTTCAGTAA CGTGAAGTAT 
GTCATGTTGG AAGAAAACAA ACAGCTTCCC AAAATGTGGC TGCACTACTT CAGAGACTGG 
CTTCAGGGAC TTCAGGATGC ATTTGACAGT GACTGGGAAA CCGGGAAAAT CATGCCAAAC 
AATTACAAGA ATGGATCAGA CGATGGAGTC CTTGCCTACA AACTCCTGGT GCAAACCGGC 
AGCCGCGATA AGCCCATCGA CATCAGCCAG TTGACTAAAC AGCGTCTGGT GGATGCAGAT 
GGCATCATTA ATCCCAGCGC TTTCTACATC TACCTGACGG CTTGGGTCAG CAACGACCCC 
GTCGCGTATG CTGCCTCCCA GGCCAACATC CGGCCACACC GACCAGAATG GGTCCACGAC 
AAAGCCGACT ACATGCCTGA AACAAGGCTG AGAATCCCGG CAGCAGAGCC CATCGAGTAT 
GCCCAGTTCC CTTTCTACCT CAACGGCTTG CGGGACACCT CAGACTTTGT GGAGGCAATT 
GAAAAAGTAA GGACCATCTG CAGCAACTAT ACGAGCCTGG GGCTGTCCAG TTACCCCAAC 
GGCTACCCCT TCCTCTTCTG GGAGCAGTAC ATCGGCCTCC GCCACTGGCT GCTGCTGTTC 
ATCAGCGTGG TGTTGGCCTG CACATTCCTC GTGTGCGCTG TCTTCCTTCT GAACCCCTGG 
ACGGCCGGGA TCATTGTGAT GGTCCTGGCG CTGATGACGG TCGAGCTGTT CGGCATGATG 
GGCCTCATCG GAATCAAGCT CAGTGCCGTG CCCGTGGTCA TCCTGATCGC TTCTGTTGGC 
ATAGGAGTGG AGTTCACCGT TCACGTTGCT TTGGCCTTTC TGACGGCCAT CGGCGACAAG 
AACCGCAGGG CTGTGCTTGC CCTGGAGCAC ATGTTTGCAC CCGTCCTGGA TGGCGCCGTG 
TCCACTCTGC TGGGAGTGCT GATGCTGGCG GGATCTGAGT TCGACTTCAT TGTCAGGTAT 
TTCTTTGCTG TGCTGGCGAT CCTCACCATC CTCGGCGTTC TCAATGGGCT GGTTTTGCTT 
CCCGTGCTTT TGTCTTTCTT TGGACCATAT CCTGAGGTGT CTCCAGCCAA CGGCTTGAAC 
CGCCTGCCCA CACCCTCCCC TGAGCCACCC CCCAGCGTGG TCCGCTTCGC CATGCCGCCC 
GGCCACACGC ACAGCGGGTC TGATTCCTCC GACTCGGAGT ATAGTTCCCA GACGACAGTG 
TCAGGCCTCA GCGAGGAGCT TCGGCACTAC GAGGCCCAGC AGGGCGCGGG AGGCCCTGCC 
CACCAAGTGA TCGTGGAAGC CACAGAAAAC CCCGTCTTCG CCCACTCCAC TGTGGTCCAT 
CCCGAATCCA GGCATCACCC ACCCTCGAAC CCGAGACAGC AGCCCCACCT GGACTCAGGG 
TCCCTGCCTC CCGGACGGCA AGGCCAGCAG CCCCGCAGGG ACCCCCCCAG AGAAGGCTTG 
TGGCCACCCC CCTACAGACC GCGCAGAGAC GCTTTTGAAA TTTCTACTGA AGGGCATTCT 
GGCCCTAGCA ATAGGGCCCG CTGGGGCCCT CGCGGGGCCC GTTCTCACAA CCCTCGGAAC 
CCAGCGTCCA CTGCCATGGG CAGCTCCGTG CCCGGCTACT GCCAGCCCAT CACCACTGTG 
ACGGCTTCTG CCTCCGTGAC TGTCGCCGTG CACCCGCCGC CTGTCCCTGG GCCTGGGCGG 
AACCCCCGAG GGGGACTCTG CCCAGGCTAC CCTGAGACTG ACCACGGCCT GTTTGAGGAC 
CCCCACGTGC CTTTCCACGT CCGGTGTGAG AGGAGGGATT CGAAGGTGGA AGTCATTGAG 
CTGCAGGACG TGGAATGCGA GGAGAGGCCC CGGGGAAGCA GCTCCAACTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PTCH1 ( NM_001083607.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001077076.1 Homo sapiens patched 1 (PTCH1), transcript variant 1e, mRNA.

Price & Availability↑

CloneID	OHu17265
Clone ID Related Accession (Same CDS sequence)	XM_005252102.2 , XM_011518872.1 , NM_001083605.1 , NM_001083604.2 , NM_001083606.2 , NM_001083606.1 , XM_011518871.1 , XM_011518869.1 , NM_001083604.1 , XM_011518870.1 , NM_001083607.1 , NM_001083606.3 , NM_001083605.2 , NM_001083607.2
Accession Version	NM_001083607.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3891bp) Protein sequence SNP
Vector	pcDNA3.1⁺/C-(K)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-04-24
Organism	Homo sapiens(Human)
Product	protein patched homolog 1 isoform S
Comment	REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AB189440.1, BF195352.1, U59464.1, AB209495.1, BX117041.1 and CR744004.1. Transcript Variant: This variant (1e) differs in the 5' UTR and has multiple differences in the 5' coding region, compared to variant 1b. These differences cause translation initiation from a downstream ATG and an isoform (S) with a shorter N-terminus, compared to isoform L. Variants 1c, 1c', 1d, and 1e encode the same isoform (S). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## CDS exon combination :: U43148.1 [ECO:0000331] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGTTTAATC CTCAACTCAT GATACAGACC CCTAAAGAAG AAGGTGCTAA TGTCCTGACC 
ACAGAAGCGC TCCTACAACA CCTGGACTCG GCACTCCAGG CCAGCCGTGT CCATGTATAC 
ATGTACAACA GGCAGTGGAA ATTGGAACAT TTGTGTTACA AATCAGGAGA GCTTATCACA 
GAAACAGGTT ACATGGATCA GATAATAGAA TATCTTTACC CTTGTTTGAT TATTACACCT 
TTGGACTGCT TCTGGGAAGG GGCGAAATTA CAGTCTGGGA CAGCATACCT CCTAGGTAAA 
CCTCCTTTGC GGTGGACAAA CTTCGACCCT TTGGAATTCC TGGAAGAGTT AAAGAAAATA 
AACTATCAAG TGGACAGCTG GGAGGAAATG CTGAATAAGG CTGAGGTTGG TCATGGTTAC 
ATGGACCGCC CCTGCCTCAA TCCGGCCGAT CCAGACTGCC CCGCCACAGC CCCCAACAAA 
AATTCAACCA AACCTCTTGA TATGGCCCTT GTTTTGAATG GTGGATGTCA TGGCTTATCC 
AGAAAGTATA TGCACTGGCA GGAGGAGTTG ATTGTGGGTG GCACAGTCAA GAACAGCACT 
GGAAAACTCG TCAGCGCCCA TGCCCTGCAG ACCATGTTCC AGTTAATGAC TCCCAAGCAA 
ATGTACGAGC ACTTCAAGGG GTACGAGTAT GTCTCACACA TCAACTGGAA CGAGGACAAA 
GCGGCAGCCA TCCTGGAGGC CTGGCAGAGG ACATATGTGG AGGTGGTTCA TCAGAGTGTC 
GCACAGAACT CCACTCAAAA GGTGCTTTCC TTCACCACCA CGACCCTGGA CGACATCCTG 
AAATCCTTCT CTGACGTCAG TGTCATCCGC GTGGCCAGCG GCTACTTACT CATGCTCGCC 
TATGCCTGTC TAACCATGCT GCGCTGGGAC TGCTCCAAGT CCCAGGGTGC CGTGGGGCTG 
GCTGGCGTCC TGCTGGTTGC ACTGTCAGTG GCTGCAGGAC TGGGCCTGTG CTCATTGATC 
GGAATTTCCT TTAACGCTGC AACAACTCAG GTTTTGCCAT TTCTCGCTCT TGGTGTTGGT 
GTGGATGATG TTTTTCTTCT GGCCCACGCC TTCAGTGAAA CAGGACAGAA TAAAAGAATC 
CCTTTTGAGG ACAGGACCGG GGAGTGCCTG AAGCGCACAG GAGCCAGCGT GGCCCTCACG 
TCCATCAGCA ATGTCACAGC CTTCTTCATG GCCGCGTTAA TCCCAATTCC CGCTCTGCGG 
GCGTTCTCCC TCCAGGCAGC GGTAGTAGTG GTGTTCAATT TTGCCATGGT TCTGCTCATT 
TTTCCTGCAA TTCTCAGCAT GGATTTATAT CGACGCGAGG ACAGGAGACT GGATATTTTC 
TGCTGTTTTA CAAGCCCCTG CGTCAGCAGA GTGATTCAGG TTGAACCTCA GGCCTACACC 
GACACACACG ACAATACCCG CTACAGCCCC CCACCTCCCT ACAGCAGCCA CAGCTTTGCC 
CATGAAACGC AGATTACCAT GCAGTCCACT GTCCAGCTCC GCACGGAGTA CGACCCCCAC 
ACGCACGTGT ACTACACCAC CGCTGAGCCG CGCTCCGAGA TCTCTGTGCA GCCCGTCACC 
GTGACACAGG ACACCCTCAG CTGCCAGAGC CCAGAGAGCA CCAGCTCCAC AAGGGACCTG 
CTCTCCCAGT TCTCCGACTC CAGCCTCCAC TGCCTCGAGC CCCCCTGTAC GAAGTGGACA 
CTCTCATCTT TTGCTGAGAA GCACTATGCT CCTTTCCTCT TGAAACCAAA AGCCAAGGTA 
GTGGTGATCT TCCTTTTTCT GGGCTTGCTG GGGGTCAGCC TTTATGGCAC CACCCGAGTG 
AGAGACGGGC TGGACCTTAC GGACATTGTA CCTCGGGAAA CCAGAGAATA TGACTTTATT 
GCTGCACAAT TCAAATACTT TTCTTTCTAC AACATGTATA TAGTCACCCA GAAAGCAGAC 
TACCCGAATA TCCAGCACTT ACTTTACGAC CTACACAGGA GTTTCAGTAA CGTGAAGTAT 
GTCATGTTGG AAGAAAACAA ACAGCTTCCC AAAATGTGGC TGCACTACTT CAGAGACTGG 
CTTCAGGGAC TTCAGGATGC ATTTGACAGT GACTGGGAAA CCGGGAAAAT CATGCCAAAC 
AATTACAAGA ATGGATCAGA CGATGGAGTC CTTGCCTACA AACTCCTGGT GCAAACCGGC 
AGCCGCGATA AGCCCATCGA CATCAGCCAG TTGACTAAAC AGCGTCTGGT GGATGCAGAT 
GGCATCATTA ATCCCAGCGC TTTCTACATC TACCTGACGG CTTGGGTCAG CAACGACCCC 
GTCGCGTATG CTGCCTCCCA GGCCAACATC CGGCCACACC GACCAGAATG GGTCCACGAC 
AAAGCCGACT ACATGCCTGA AACAAGGCTG AGAATCCCGG CAGCAGAGCC CATCGAGTAT 
GCCCAGTTCC CTTTCTACCT CAACGGCTTG CGGGACACCT CAGACTTTGT GGAGGCAATT 
GAAAAAGTAA GGACCATCTG CAGCAACTAT ACGAGCCTGG GGCTGTCCAG TTACCCCAAC 
GGCTACCCCT TCCTCTTCTG GGAGCAGTAC ATCGGCCTCC GCCACTGGCT GCTGCTGTTC 
ATCAGCGTGG TGTTGGCCTG CACATTCCTC GTGTGCGCTG TCTTCCTTCT GAACCCCTGG 
ACGGCCGGGA TCATTGTGAT GGTCCTGGCG CTGATGACGG TCGAGCTGTT CGGCATGATG 
GGCCTCATCG GAATCAAGCT CAGTGCCGTG CCCGTGGTCA TCCTGATCGC TTCTGTTGGC 
ATAGGAGTGG AGTTCACCGT TCACGTTGCT TTGGCCTTTC TGACGGCCAT CGGCGACAAG 
AACCGCAGGG CTGTGCTTGC CCTGGAGCAC ATGTTTGCAC CCGTCCTGGA TGGCGCCGTG 
TCCACTCTGC TGGGAGTGCT GATGCTGGCG GGATCTGAGT TCGACTTCAT TGTCAGGTAT 
TTCTTTGCTG TGCTGGCGAT CCTCACCATC CTCGGCGTTC TCAATGGGCT GGTTTTGCTT 
CCCGTGCTTT TGTCTTTCTT TGGACCATAT CCTGAGGTGT CTCCAGCCAA CGGCTTGAAC 
CGCCTGCCCA CACCCTCCCC TGAGCCACCC CCCAGCGTGG TCCGCTTCGC CATGCCGCCC 
GGCCACACGC ACAGCGGGTC TGATTCCTCC GACTCGGAGT ATAGTTCCCA GACGACAGTG 
TCAGGCCTCA GCGAGGAGCT TCGGCACTAC GAGGCCCAGC AGGGCGCGGG AGGCCCTGCC 
CACCAAGTGA TCGTGGAAGC CACAGAAAAC CCCGTCTTCG CCCACTCCAC TGTGGTCCAT 
CCCGAATCCA GGCATCACCC ACCCTCGAAC CCGAGACAGC AGCCCCACCT GGACTCAGGG 
TCCCTGCCTC CCGGACGGCA AGGCCAGCAG CCCCGCAGGG ACCCCCCCAG AGAAGGCTTG 
TGGCCACCCC CCTACAGACC GCGCAGAGAC GCTTTTGAAA TTTCTACTGA AGGGCATTCT 
GGCCCTAGCA ATAGGGCCCG CTGGGGCCCT CGCGGGGCCC GTTCTCACAA CCCTCGGAAC 
CCAGCGTCCA CTGCCATGGG CAGCTCCGTG CCCGGCTACT GCCAGCCCAT CACCACTGTG 
ACGGCTTCTG CCTCCGTGAC TGTCGCCGTG CACCCGCCGC CTGTCCCTGG GCCTGGGCGG 
AACCCCCGAG GGGGACTCTG CCCAGGCTAC CCTGAGACTG ACCACGGCCT GTTTGAGGAC 
CCCCACGTGC CTTTCCACGT CCGGTGTGAG AGGAGGGATT CGAAGGTGGA AGTCATTGAG 
CTGCAGGACG TGGAATGCGA GGAGAGGCCC CGGGGAAGCA GCTCCAACTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001077076.1
CDS	516..4406
Translation	MFNPQLMIQTPKEEGANVLTTEALLQHLDSALQASRVHVYMYNRQWKLEHLCYKSGELITETGYMDQIIEYLYPCLIITPLDCFWEGAKLQSGTAYLLGKPPLRWTNFDPLEFLEELKKINYQVDSWEEMLNKAEVGHGYMDRPCLNPADPDCPATAPNKNSTKPLDMALVLNGGCHGLSRKYMHWQEELIVGGTVKNSTGKLVSAHALQTMFQLMTPKQMYEHFKGYEYVSHINWNEDKAAAILEAWQRTYVEVVHQSVAQNSTQKVLSFTTTTLDDILKSFSDVSVIRVASGYLLMLAYACLTMLRWDCSKSQGAVGLAGVLLVALSVAAGLGLCSLIGISFNAATTQVLPFLALGVGVDDVFLLAHAFSETGQNKRIPFEDRTGECLKRTGASVALTSISNVTAFFMAALIPIPALRAFSLQAAVVVVFNFAMVLLIFPAILSMDLYRREDRRLDIFCCFTSPCVSRVIQVEPQAYTDTHDNTRYSPPPPYSSHSFAHETQITMQSTVQLRTEYDPHTHVYYTTAEPRSEISVQPVTVTQDTLSCQSPESTSSTRDLLSQFSDSSLHCLEPPCTKWTLSSFAEKHYAPFLLKPKAKVVVIFLFLGLLGVSLYGTTRVRDGLDLTDIVPRETREYDFIAAQFKYFSFYNMYIVTQKADYPNIQHLLYDLHRSFSNVKYVMLEENKQLPKMWLHYFRDWLQGLQDAFDSDWETGKIMPNNYKNGSDDGVLAYKLLVQTGSRDKPIDISQLTKQRLVDADGIINPSAFYIYLTAWVSNDPVAYAASQANIRPHRPEWVHDKADYMPETRLRIPAAEPIEYAQFPFYLNGLRDTSDFVEAIEKVRTICSNYTSLGLSSYPNGYPFLFWEQYIGLRHWLLLFISVVLACTFLVCAVFLLNPWTAGIIVMVLALMTVELFGMMGLIGIKLSAVPVVILIASVGIGVEFTVHVALAFLTAIGDKNRRAVLALEHMFAPVLDGAVSTLLGVLMLAGSEFDFIVRYFFAVLAILTILGVLNGLVLLPVLLSFFGPYPEVSPANGLNRLPTPSPEPPPSVVRFAMPPGHTHSGSDSSDSEYSSQTTVSGLSEELRHYEAQQGAGGPAHQVIVEATENPVFAHSTVVHPESRHHPPSNPRQQPHLDSGSLPPGRQGQQPRRDPPREGLWPPPYRPRRDAFEISTEGHSGPSNRARWGPRGARSHNPRNPASTAMGSSVPGYCQPITTVTASASVTVAVHPPPVPGPGRNPRGGLCPGYPETDHGLFEDPHVPFHVRCERRDSKVEVIELQDVECEERPRGSSSN

Target ORF information:

RefSeq Version	NM_001083607.1
Organism	Homo sapiens(Human)
Definition	Homo sapiens patched 1 (PTCH1), transcript variant 1e, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001083607.1

ORF Insert Sequence:

ATGTTTAATC CTCAACTCAT GATACAGACC CCTAAAGAAG AAGGTGCTAA TGTCCTGACC 
ACAGAAGCGC TCCTACAACA CCTGGACTCG GCACTCCAGG CCAGCCGTGT CCATGTATAC 
ATGTACAACA GGCAGTGGAA ATTGGAACAT TTGTGTTACA AATCAGGAGA GCTTATCACA 
GAAACAGGTT ACATGGATCA GATAATAGAA TATCTTTACC CTTGTTTGAT TATTACACCT 
TTGGACTGCT TCTGGGAAGG GGCGAAATTA CAGTCTGGGA CAGCATACCT CCTAGGTAAA 
CCTCCTTTGC GGTGGACAAA CTTCGACCCT TTGGAATTCC TGGAAGAGTT AAAGAAAATA 
AACTATCAAG TGGACAGCTG GGAGGAAATG CTGAATAAGG CTGAGGTTGG TCATGGTTAC 
ATGGACCGCC CCTGCCTCAA TCCGGCCGAT CCAGACTGCC CCGCCACAGC CCCCAACAAA 
AATTCAACCA AACCTCTTGA TATGGCCCTT GTTTTGAATG GTGGATGTCA TGGCTTATCC 
AGAAAGTATA TGCACTGGCA GGAGGAGTTG ATTGTGGGTG GCACAGTCAA GAACAGCACT 
GGAAAACTCG TCAGCGCCCA TGCCCTGCAG ACCATGTTCC AGTTAATGAC TCCCAAGCAA 
ATGTACGAGC ACTTCAAGGG GTACGAGTAT GTCTCACACA TCAACTGGAA CGAGGACAAA 
GCGGCAGCCA TCCTGGAGGC CTGGCAGAGG ACATATGTGG AGGTGGTTCA TCAGAGTGTC 
GCACAGAACT CCACTCAAAA GGTGCTTTCC TTCACCACCA CGACCCTGGA CGACATCCTG 
AAATCCTTCT CTGACGTCAG TGTCATCCGC GTGGCCAGCG GCTACTTACT CATGCTCGCC 
TATGCCTGTC TAACCATGCT GCGCTGGGAC TGCTCCAAGT CCCAGGGTGC CGTGGGGCTG 
GCTGGCGTCC TGCTGGTTGC ACTGTCAGTG GCTGCAGGAC TGGGCCTGTG CTCATTGATC 
GGAATTTCCT TTAACGCTGC AACAACTCAG GTTTTGCCAT TTCTCGCTCT TGGTGTTGGT 
GTGGATGATG TTTTTCTTCT GGCCCACGCC TTCAGTGAAA CAGGACAGAA TAAAAGAATC 
CCTTTTGAGG ACAGGACCGG GGAGTGCCTG AAGCGCACAG GAGCCAGCGT GGCCCTCACG 
TCCATCAGCA ATGTCACAGC CTTCTTCATG GCCGCGTTAA TCCCAATTCC CGCTCTGCGG 
GCGTTCTCCC TCCAGGCAGC GGTAGTAGTG GTGTTCAATT TTGCCATGGT TCTGCTCATT 
TTTCCTGCAA TTCTCAGCAT GGATTTATAT CGACGCGAGG ACAGGAGACT GGATATTTTC 
TGCTGTTTTA CAAGCCCCTG CGTCAGCAGA GTGATTCAGG TTGAACCTCA GGCCTACACC 
GACACACACG ACAATACCCG CTACAGCCCC CCACCTCCCT ACAGCAGCCA CAGCTTTGCC 
CATGAAACGC AGATTACCAT GCAGTCCACT GTCCAGCTCC GCACGGAGTA CGACCCCCAC 
ACGCACGTGT ACTACACCAC CGCTGAGCCG CGCTCCGAGA TCTCTGTGCA GCCCGTCACC 
GTGACACAGG ACACCCTCAG CTGCCAGAGC CCAGAGAGCA CCAGCTCCAC AAGGGACCTG 
CTCTCCCAGT TCTCCGACTC CAGCCTCCAC TGCCTCGAGC CCCCCTGTAC GAAGTGGACA 
CTCTCATCTT TTGCTGAGAA GCACTATGCT CCTTTCCTCT TGAAACCAAA AGCCAAGGTA 
GTGGTGATCT TCCTTTTTCT GGGCTTGCTG GGGGTCAGCC TTTATGGCAC CACCCGAGTG 
AGAGACGGGC TGGACCTTAC GGACATTGTA CCTCGGGAAA CCAGAGAATA TGACTTTATT 
GCTGCACAAT TCAAATACTT TTCTTTCTAC AACATGTATA TAGTCACCCA GAAAGCAGAC 
TACCCGAATA TCCAGCACTT ACTTTACGAC CTACACAGGA GTTTCAGTAA CGTGAAGTAT 
GTCATGTTGG AAGAAAACAA ACAGCTTCCC AAAATGTGGC TGCACTACTT CAGAGACTGG 
CTTCAGGGAC TTCAGGATGC ATTTGACAGT GACTGGGAAA CCGGGAAAAT CATGCCAAAC 
AATTACAAGA ATGGATCAGA CGATGGAGTC CTTGCCTACA AACTCCTGGT GCAAACCGGC 
AGCCGCGATA AGCCCATCGA CATCAGCCAG TTGACTAAAC AGCGTCTGGT GGATGCAGAT 
GGCATCATTA ATCCCAGCGC TTTCTACATC TACCTGACGG CTTGGGTCAG CAACGACCCC 
GTCGCGTATG CTGCCTCCCA GGCCAACATC CGGCCACACC GACCAGAATG GGTCCACGAC 
AAAGCCGACT ACATGCCTGA AACAAGGCTG AGAATCCCGG CAGCAGAGCC CATCGAGTAT 
GCCCAGTTCC CTTTCTACCT CAACGGCTTG CGGGACACCT CAGACTTTGT GGAGGCAATT 
GAAAAAGTAA GGACCATCTG CAGCAACTAT ACGAGCCTGG GGCTGTCCAG TTACCCCAAC 
GGCTACCCCT TCCTCTTCTG GGAGCAGTAC ATCGGCCTCC GCCACTGGCT GCTGCTGTTC 
ATCAGCGTGG TGTTGGCCTG CACATTCCTC GTGTGCGCTG TCTTCCTTCT GAACCCCTGG 
ACGGCCGGGA TCATTGTGAT GGTCCTGGCG CTGATGACGG TCGAGCTGTT CGGCATGATG 
GGCCTCATCG GAATCAAGCT CAGTGCCGTG CCCGTGGTCA TCCTGATCGC TTCTGTTGGC 
ATAGGAGTGG AGTTCACCGT TCACGTTGCT TTGGCCTTTC TGACGGCCAT CGGCGACAAG 
AACCGCAGGG CTGTGCTTGC CCTGGAGCAC ATGTTTGCAC CCGTCCTGGA TGGCGCCGTG 
TCCACTCTGC TGGGAGTGCT GATGCTGGCG GGATCTGAGT TCGACTTCAT TGTCAGGTAT 
TTCTTTGCTG TGCTGGCGAT CCTCACCATC CTCGGCGTTC TCAATGGGCT GGTTTTGCTT 
CCCGTGCTTT TGTCTTTCTT TGGACCATAT CCTGAGGTGT CTCCAGCCAA CGGCTTGAAC 
CGCCTGCCCA CACCCTCCCC TGAGCCACCC CCCAGCGTGG TCCGCTTCGC CATGCCGCCC 
GGCCACACGC ACAGCGGGTC TGATTCCTCC GACTCGGAGT ATAGTTCCCA GACGACAGTG 
TCAGGCCTCA GCGAGGAGCT TCGGCACTAC GAGGCCCAGC AGGGCGCGGG AGGCCCTGCC 
CACCAAGTGA TCGTGGAAGC CACAGAAAAC CCCGTCTTCG CCCACTCCAC TGTGGTCCAT 
CCCGAATCCA GGCATCACCC ACCCTCGAAC CCGAGACAGC AGCCCCACCT GGACTCAGGG 
TCCCTGCCTC CCGGACGGCA AGGCCAGCAG CCCCGCAGGG ACCCCCCCAG AGAAGGCTTG 
TGGCCACCCC CCTACAGACC GCGCAGAGAC GCTTTTGAAA TTTCTACTGA AGGGCATTCT 
GGCCCTAGCA ATAGGGCCCG CTGGGGCCCT CGCGGGGCCC GTTCTCACAA CCCTCGGAAC 
CCAGCGTCCA CTGCCATGGG CAGCTCCGTG CCCGGCTACT GCCAGCCCAT CACCACTGTG 
ACGGCTTCTG CCTCCGTGAC TGTCGCCGTG CACCCGCCGC CTGTCCCTGG GCCTGGGCGG 
AACCCCCGAG GGGGACTCTG CCCAGGCTAC CCTGAGACTG ACCACGGCCT GTTTGAGGAC 
CCCCACGTGC CTTTCCACGT CCGGTGTGAG AGGAGGGATT CGAAGGTGGA AGTCATTGAG 
CTGCAGGACG TGGAATGCGA GGAGAGGCCC CGGGGAAGCA GCTCCAACTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PTCH1 ( NM_001083606.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_001077075.1 Homo sapiens patched 1 (PTCH1), transcript variant 1d, mRNA.

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CloneID	OHu17265
Clone ID Related Accession (Same CDS sequence)	XM_005252102.2 , XM_011518872.1 , NM_001083605.1 , NM_001083604.2 , NM_001083606.2 , NM_001083606.1 , XM_011518871.1 , XM_011518869.1 , NM_001083604.1 , XM_011518870.1 , NM_001083607.1 , NM_001083606.3 , NM_001083605.2 , NM_001083607.2
Accession Version	NM_001083606.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3891bp) Protein sequence SNP
Vector	pcDNA3.1⁺/C-(K)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2020-01-03
Organism	Homo sapiens(human)
Product	protein patched homolog 1 isoform S
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL161729.27, U43148.1, AI358880.1, AB209495.1, BX117041.1, CR744004.1 and AI417736.1. On Jun 2, 2019 this sequence version replaced NM_001083606.2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: U43148.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGTTTAATC CTCAACTCAT GATACAGACC CCTAAAGAAG AAGGTGCTAA TGTCCTGACC 
ACAGAAGCGC TCCTACAACA CCTGGACTCG GCACTCCAGG CCAGCCGTGT CCATGTATAC 
ATGTACAACA GGCAGTGGAA ATTGGAACAT TTGTGTTACA AATCAGGAGA GCTTATCACA 
GAAACAGGTT ACATGGATCA GATAATAGAA TATCTTTACC CTTGTTTGAT TATTACACCT 
TTGGACTGCT TCTGGGAAGG GGCGAAATTA CAGTCTGGGA CAGCATACCT CCTAGGTAAA 
CCTCCTTTGC GGTGGACAAA CTTCGACCCT TTGGAATTCC TGGAAGAGTT AAAGAAAATA 
AACTATCAAG TGGACAGCTG GGAGGAAATG CTGAATAAGG CTGAGGTTGG TCATGGTTAC 
ATGGACCGCC CCTGCCTCAA TCCGGCCGAT CCAGACTGCC CCGCCACAGC CCCCAACAAA 
AATTCAACCA AACCTCTTGA TATGGCCCTT GTTTTGAATG GTGGATGTCA TGGCTTATCC 
AGAAAGTATA TGCACTGGCA GGAGGAGTTG ATTGTGGGTG GCACAGTCAA GAACAGCACT 
GGAAAACTCG TCAGCGCCCA TGCCCTGCAG ACCATGTTCC AGTTAATGAC TCCCAAGCAA 
ATGTACGAGC ACTTCAAGGG GTACGAGTAT GTCTCACACA TCAACTGGAA CGAGGACAAA 
GCGGCAGCCA TCCTGGAGGC CTGGCAGAGG ACATATGTGG AGGTGGTTCA TCAGAGTGTC 
GCACAGAACT CCACTCAAAA GGTGCTTTCC TTCACCACCA CGACCCTGGA CGACATCCTG 
AAATCCTTCT CTGACGTCAG TGTCATCCGC GTGGCCAGCG GCTACTTACT CATGCTCGCC 
TATGCCTGTC TAACCATGCT GCGCTGGGAC TGCTCCAAGT CCCAGGGTGC CGTGGGGCTG 
GCTGGCGTCC TGCTGGTTGC ACTGTCAGTG GCTGCAGGAC TGGGCCTGTG CTCATTGATC 
GGAATTTCCT TTAACGCTGC AACAACTCAG GTTTTGCCAT TTCTCGCTCT TGGTGTTGGT 
GTGGATGATG TTTTTCTTCT GGCCCACGCC TTCAGTGAAA CAGGACAGAA TAAAAGAATC 
CCTTTTGAGG ACAGGACCGG GGAGTGCCTG AAGCGCACAG GAGCCAGCGT GGCCCTCACG 
TCCATCAGCA ATGTCACAGC CTTCTTCATG GCCGCGTTAA TCCCAATTCC CGCTCTGCGG 
GCGTTCTCCC TCCAGGCAGC GGTAGTAGTG GTGTTCAATT TTGCCATGGT TCTGCTCATT 
TTTCCTGCAA TTCTCAGCAT GGATTTATAT CGACGCGAGG ACAGGAGACT GGATATTTTC 
TGCTGTTTTA CAAGCCCCTG CGTCAGCAGA GTGATTCAGG TTGAACCTCA GGCCTACACC 
GACACACACG ACAATACCCG CTACAGCCCC CCACCTCCCT ACAGCAGCCA CAGCTTTGCC 
CATGAAACGC AGATTACCAT GCAGTCCACT GTCCAGCTCC GCACGGAGTA CGACCCCCAC 
ACGCACGTGT ACTACACCAC CGCTGAGCCG CGCTCCGAGA TCTCTGTGCA GCCCGTCACC 
GTGACACAGG ACACCCTCAG CTGCCAGAGC CCAGAGAGCA CCAGCTCCAC AAGGGACCTG 
CTCTCCCAGT TCTCCGACTC CAGCCTCCAC TGCCTCGAGC CCCCCTGTAC GAAGTGGACA 
CTCTCATCTT TTGCTGAGAA GCACTATGCT CCTTTCCTCT TGAAACCAAA AGCCAAGGTA 
GTGGTGATCT TCCTTTTTCT GGGCTTGCTG GGGGTCAGCC TTTATGGCAC CACCCGAGTG 
AGAGACGGGC TGGACCTTAC GGACATTGTA CCTCGGGAAA CCAGAGAATA TGACTTTATT 
GCTGCACAAT TCAAATACTT TTCTTTCTAC AACATGTATA TAGTCACCCA GAAAGCAGAC 
TACCCGAATA TCCAGCACTT ACTTTACGAC CTACACAGGA GTTTCAGTAA CGTGAAGTAT 
GTCATGTTGG AAGAAAACAA ACAGCTTCCC AAAATGTGGC TGCACTACTT CAGAGACTGG 
CTTCAGGGAC TTCAGGATGC ATTTGACAGT GACTGGGAAA CCGGGAAAAT CATGCCAAAC 
AATTACAAGA ATGGATCAGA CGATGGAGTC CTTGCCTACA AACTCCTGGT GCAAACCGGC 
AGCCGCGATA AGCCCATCGA CATCAGCCAG TTGACTAAAC AGCGTCTGGT GGATGCAGAT 
GGCATCATTA ATCCCAGCGC TTTCTACATC TACCTGACGG CTTGGGTCAG CAACGACCCC 
GTCGCGTATG CTGCCTCCCA GGCCAACATC CGGCCACACC GACCAGAATG GGTCCACGAC 
AAAGCCGACT ACATGCCTGA AACAAGGCTG AGAATCCCGG CAGCAGAGCC CATCGAGTAT 
GCCCAGTTCC CTTTCTACCT CAACGGCTTG CGGGACACCT CAGACTTTGT GGAGGCAATT 
GAAAAAGTAA GGACCATCTG CAGCAACTAT ACGAGCCTGG GGCTGTCCAG TTACCCCAAC 
GGCTACCCCT TCCTCTTCTG GGAGCAGTAC ATCGGCCTCC GCCACTGGCT GCTGCTGTTC 
ATCAGCGTGG TGTTGGCCTG CACATTCCTC GTGTGCGCTG TCTTCCTTCT GAACCCCTGG 
ACGGCCGGGA TCATTGTGAT GGTCCTGGCG CTGATGACGG TCGAGCTGTT CGGCATGATG 
GGCCTCATCG GAATCAAGCT CAGTGCCGTG CCCGTGGTCA TCCTGATCGC TTCTGTTGGC 
ATAGGAGTGG AGTTCACCGT TCACGTTGCT TTGGCCTTTC TGACGGCCAT CGGCGACAAG 
AACCGCAGGG CTGTGCTTGC CCTGGAGCAC ATGTTTGCAC CCGTCCTGGA TGGCGCCGTG 
TCCACTCTGC TGGGAGTGCT GATGCTGGCG GGATCTGAGT TCGACTTCAT TGTCAGGTAT 
TTCTTTGCTG TGCTGGCGAT CCTCACCATC CTCGGCGTTC TCAATGGGCT GGTTTTGCTT 
CCCGTGCTTT TGTCTTTCTT TGGACCATAT CCTGAGGTGT CTCCAGCCAA CGGCTTGAAC 
CGCCTGCCCA CACCCTCCCC TGAGCCACCC CCCAGCGTGG TCCGCTTCGC CATGCCGCCC 
GGCCACACGC ACAGCGGGTC TGATTCCTCC GACTCGGAGT ATAGTTCCCA GACGACAGTG 
TCAGGCCTCA GCGAGGAGCT TCGGCACTAC GAGGCCCAGC AGGGCGCGGG AGGCCCTGCC 
CACCAAGTGA TCGTGGAAGC CACAGAAAAC CCCGTCTTCG CCCACTCCAC TGTGGTCCAT 
CCCGAATCCA GGCATCACCC ACCCTCGAAC CCGAGACAGC AGCCCCACCT GGACTCAGGG 
TCCCTGCCTC CCGGACGGCA AGGCCAGCAG CCCCGCAGGG ACCCCCCCAG AGAAGGCTTG 
TGGCCACCCC CCTACAGACC GCGCAGAGAC GCTTTTGAAA TTTCTACTGA AGGGCATTCT 
GGCCCTAGCA ATAGGGCCCG CTGGGGCCCT CGCGGGGCCC GTTCTCACAA CCCTCGGAAC 
CCAGCGTCCA CTGCCATGGG CAGCTCCGTG CCCGGCTACT GCCAGCCCAT CACCACTGTG 
ACGGCTTCTG CCTCCGTGAC TGTCGCCGTG CACCCGCCGC CTGTCCCTGG GCCTGGGCGG 
AACCCCCGAG GGGGACTCTG CCCAGGCTAC CCTGAGACTG ACCACGGCCT GTTTGAGGAC 
CCCCACGTGC CTTTCCACGT CCGGTGTGAG AGGAGGGATT CGAAGGTGGA AGTCATTGAG 
CTGCAGGACG TGGAATGCGA GGAGAGGCCC CGGGGAAGCA GCTCCAACTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001077075.1
CDS	452..4342
Translation	MFNPQLMIQTPKEEGANVLTTEALLQHLDSALQASRVHVYMYNRQWKLEHLCYKSGELITETGYMDQIIEYLYPCLIITPLDCFWEGAKLQSGTAYLLGKPPLRWTNFDPLEFLEELKKINYQVDSWEEMLNKAEVGHGYMDRPCLNPADPDCPATAPNKNSTKPLDMALVLNGGCHGLSRKYMHWQEELIVGGTVKNSTGKLVSAHALQTMFQLMTPKQMYEHFKGYEYVSHINWNEDKAAAILEAWQRTYVEVVHQSVAQNSTQKVLSFTTTTLDDILKSFSDVSVIRVASGYLLMLAYACLTMLRWDCSKSQGAVGLAGVLLVALSVAAGLGLCSLIGISFNAATTQVLPFLALGVGVDDVFLLAHAFSETGQNKRIPFEDRTGECLKRTGASVALTSISNVTAFFMAALIPIPALRAFSLQAAVVVVFNFAMVLLIFPAILSMDLYRREDRRLDIFCCFTSPCVSRVIQVEPQAYTDTHDNTRYSPPPPYSSHSFAHETQITMQSTVQLRTEYDPHTHVYYTTAEPRSEISVQPVTVTQDTLSCQSPESTSSTRDLLSQFSDSSLHCLEPPCTKWTLSSFAEKHYAPFLLKPKAKVVVIFLFLGLLGVSLYGTTRVRDGLDLTDIVPRETREYDFIAAQFKYFSFYNMYIVTQKADYPNIQHLLYDLHRSFSNVKYVMLEENKQLPKMWLHYFRDWLQGLQDAFDSDWETGKIMPNNYKNGSDDGVLAYKLLVQTGSRDKPIDISQLTKQRLVDADGIINPSAFYIYLTAWVSNDPVAYAASQANIRPHRPEWVHDKADYMPETRLRIPAAEPIEYAQFPFYLNGLRDTSDFVEAIEKVRTICSNYTSLGLSSYPNGYPFLFWEQYIGLRHWLLLFISVVLACTFLVCAVFLLNPWTAGIIVMVLALMTVELFGMMGLIGIKLSAVPVVILIASVGIGVEFTVHVALAFLTAIGDKNRRAVLALEHMFAPVLDGAVSTLLGVLMLAGSEFDFIVRYFFAVLAILTILGVLNGLVLLPVLLSFFGPYPEVSPANGLNRLPTPSPEPPPSVVRFAMPPGHTHSGSDSSDSEYSSQTTVSGLSEELRHYEAQQGAGGPAHQVIVEATENPVFAHSTVVHPESRHHPPSNPRQQPHLDSGSLPPGRQGQQPRRDPPREGLWPPPYRPRRDAFEISTEGHSGPSNRARWGPRGARSHNPRNPASTAMGSSVPGYCQPITTVTASASVTVAVHPPPVPGPGRNPRGGLCPGYPETDHGLFEDPHVPFHVRCERRDSKVEVIELQDVECEERPRGSSSN

Target ORF information:

RefSeq Version	NM_001083606.3
Organism	Homo sapiens(human)
Definition	Homo sapiens patched 1 (PTCH1), transcript variant 1d, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001083606.3

ORF Insert Sequence:

ATGTTTAATC CTCAACTCAT GATACAGACC CCTAAAGAAG AAGGTGCTAA TGTCCTGACC 
ACAGAAGCGC TCCTACAACA CCTGGACTCG GCACTCCAGG CCAGCCGTGT CCATGTATAC 
ATGTACAACA GGCAGTGGAA ATTGGAACAT TTGTGTTACA AATCAGGAGA GCTTATCACA 
GAAACAGGTT ACATGGATCA GATAATAGAA TATCTTTACC CTTGTTTGAT TATTACACCT 
TTGGACTGCT TCTGGGAAGG GGCGAAATTA CAGTCTGGGA CAGCATACCT CCTAGGTAAA 
CCTCCTTTGC GGTGGACAAA CTTCGACCCT TTGGAATTCC TGGAAGAGTT AAAGAAAATA 
AACTATCAAG TGGACAGCTG GGAGGAAATG CTGAATAAGG CTGAGGTTGG TCATGGTTAC 
ATGGACCGCC CCTGCCTCAA TCCGGCCGAT CCAGACTGCC CCGCCACAGC CCCCAACAAA 
AATTCAACCA AACCTCTTGA TATGGCCCTT GTTTTGAATG GTGGATGTCA TGGCTTATCC 
AGAAAGTATA TGCACTGGCA GGAGGAGTTG ATTGTGGGTG GCACAGTCAA GAACAGCACT 
GGAAAACTCG TCAGCGCCCA TGCCCTGCAG ACCATGTTCC AGTTAATGAC TCCCAAGCAA 
ATGTACGAGC ACTTCAAGGG GTACGAGTAT GTCTCACACA TCAACTGGAA CGAGGACAAA 
GCGGCAGCCA TCCTGGAGGC CTGGCAGAGG ACATATGTGG AGGTGGTTCA TCAGAGTGTC 
GCACAGAACT CCACTCAAAA GGTGCTTTCC TTCACCACCA CGACCCTGGA CGACATCCTG 
AAATCCTTCT CTGACGTCAG TGTCATCCGC GTGGCCAGCG GCTACTTACT CATGCTCGCC 
TATGCCTGTC TAACCATGCT GCGCTGGGAC TGCTCCAAGT CCCAGGGTGC CGTGGGGCTG 
GCTGGCGTCC TGCTGGTTGC ACTGTCAGTG GCTGCAGGAC TGGGCCTGTG CTCATTGATC 
GGAATTTCCT TTAACGCTGC AACAACTCAG GTTTTGCCAT TTCTCGCTCT TGGTGTTGGT 
GTGGATGATG TTTTTCTTCT GGCCCACGCC TTCAGTGAAA CAGGACAGAA TAAAAGAATC 
CCTTTTGAGG ACAGGACCGG GGAGTGCCTG AAGCGCACAG GAGCCAGCGT GGCCCTCACG 
TCCATCAGCA ATGTCACAGC CTTCTTCATG GCCGCGTTAA TCCCAATTCC CGCTCTGCGG 
GCGTTCTCCC TCCAGGCAGC GGTAGTAGTG GTGTTCAATT TTGCCATGGT TCTGCTCATT 
TTTCCTGCAA TTCTCAGCAT GGATTTATAT CGACGCGAGG ACAGGAGACT GGATATTTTC 
TGCTGTTTTA CAAGCCCCTG CGTCAGCAGA GTGATTCAGG TTGAACCTCA GGCCTACACC 
GACACACACG ACAATACCCG CTACAGCCCC CCACCTCCCT ACAGCAGCCA CAGCTTTGCC 
CATGAAACGC AGATTACCAT GCAGTCCACT GTCCAGCTCC GCACGGAGTA CGACCCCCAC 
ACGCACGTGT ACTACACCAC CGCTGAGCCG CGCTCCGAGA TCTCTGTGCA GCCCGTCACC 
GTGACACAGG ACACCCTCAG CTGCCAGAGC CCAGAGAGCA CCAGCTCCAC AAGGGACCTG 
CTCTCCCAGT TCTCCGACTC CAGCCTCCAC TGCCTCGAGC CCCCCTGTAC GAAGTGGACA 
CTCTCATCTT TTGCTGAGAA GCACTATGCT CCTTTCCTCT TGAAACCAAA AGCCAAGGTA 
GTGGTGATCT TCCTTTTTCT GGGCTTGCTG GGGGTCAGCC TTTATGGCAC CACCCGAGTG 
AGAGACGGGC TGGACCTTAC GGACATTGTA CCTCGGGAAA CCAGAGAATA TGACTTTATT 
GCTGCACAAT TCAAATACTT TTCTTTCTAC AACATGTATA TAGTCACCCA GAAAGCAGAC 
TACCCGAATA TCCAGCACTT ACTTTACGAC CTACACAGGA GTTTCAGTAA CGTGAAGTAT 
GTCATGTTGG AAGAAAACAA ACAGCTTCCC AAAATGTGGC TGCACTACTT CAGAGACTGG 
CTTCAGGGAC TTCAGGATGC ATTTGACAGT GACTGGGAAA CCGGGAAAAT CATGCCAAAC 
AATTACAAGA ATGGATCAGA CGATGGAGTC CTTGCCTACA AACTCCTGGT GCAAACCGGC 
AGCCGCGATA AGCCCATCGA CATCAGCCAG TTGACTAAAC AGCGTCTGGT GGATGCAGAT 
GGCATCATTA ATCCCAGCGC TTTCTACATC TACCTGACGG CTTGGGTCAG CAACGACCCC 
GTCGCGTATG CTGCCTCCCA GGCCAACATC CGGCCACACC GACCAGAATG GGTCCACGAC 
AAAGCCGACT ACATGCCTGA AACAAGGCTG AGAATCCCGG CAGCAGAGCC CATCGAGTAT 
GCCCAGTTCC CTTTCTACCT CAACGGCTTG CGGGACACCT CAGACTTTGT GGAGGCAATT 
GAAAAAGTAA GGACCATCTG CAGCAACTAT ACGAGCCTGG GGCTGTCCAG TTACCCCAAC 
GGCTACCCCT TCCTCTTCTG GGAGCAGTAC ATCGGCCTCC GCCACTGGCT GCTGCTGTTC 
ATCAGCGTGG TGTTGGCCTG CACATTCCTC GTGTGCGCTG TCTTCCTTCT GAACCCCTGG 
ACGGCCGGGA TCATTGTGAT GGTCCTGGCG CTGATGACGG TCGAGCTGTT CGGCATGATG 
GGCCTCATCG GAATCAAGCT CAGTGCCGTG CCCGTGGTCA TCCTGATCGC TTCTGTTGGC 
ATAGGAGTGG AGTTCACCGT TCACGTTGCT TTGGCCTTTC TGACGGCCAT CGGCGACAAG 
AACCGCAGGG CTGTGCTTGC CCTGGAGCAC ATGTTTGCAC CCGTCCTGGA TGGCGCCGTG 
TCCACTCTGC TGGGAGTGCT GATGCTGGCG GGATCTGAGT TCGACTTCAT TGTCAGGTAT 
TTCTTTGCTG TGCTGGCGAT CCTCACCATC CTCGGCGTTC TCAATGGGCT GGTTTTGCTT 
CCCGTGCTTT TGTCTTTCTT TGGACCATAT CCTGAGGTGT CTCCAGCCAA CGGCTTGAAC 
CGCCTGCCCA CACCCTCCCC TGAGCCACCC CCCAGCGTGG TCCGCTTCGC CATGCCGCCC 
GGCCACACGC ACAGCGGGTC TGATTCCTCC GACTCGGAGT ATAGTTCCCA GACGACAGTG 
TCAGGCCTCA GCGAGGAGCT TCGGCACTAC GAGGCCCAGC AGGGCGCGGG AGGCCCTGCC 
CACCAAGTGA TCGTGGAAGC CACAGAAAAC CCCGTCTTCG CCCACTCCAC TGTGGTCCAT 
CCCGAATCCA GGCATCACCC ACCCTCGAAC CCGAGACAGC AGCCCCACCT GGACTCAGGG 
TCCCTGCCTC CCGGACGGCA AGGCCAGCAG CCCCGCAGGG ACCCCCCCAG AGAAGGCTTG 
TGGCCACCCC CCTACAGACC GCGCAGAGAC GCTTTTGAAA TTTCTACTGA AGGGCATTCT 
GGCCCTAGCA ATAGGGCCCG CTGGGGCCCT CGCGGGGCCC GTTCTCACAA CCCTCGGAAC 
CCAGCGTCCA CTGCCATGGG CAGCTCCGTG CCCGGCTACT GCCAGCCCAT CACCACTGTG 
ACGGCTTCTG CCTCCGTGAC TGTCGCCGTG CACCCGCCGC CTGTCCCTGG GCCTGGGCGG 
AACCCCCGAG GGGGACTCTG CCCAGGCTAC CCTGAGACTG ACCACGGCCT GTTTGAGGAC 
CCCCACGTGC CTTTCCACGT CCGGTGTGAG AGGAGGGATT CGAAGGTGGA AGTCATTGAG 
CTGCAGGACG TGGAATGCGA GGAGAGGCCC CGGGGAAGCA GCTCCAACTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PTCH1 ( NM_001083605.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001077074.1 Homo sapiens patched 1 (PTCH1), transcript variant 1c', mRNA.

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CloneID	OHu17265
Clone ID Related Accession (Same CDS sequence)	XM_005252102.2 , XM_011518872.1 , NM_001083605.1 , NM_001083604.2 , NM_001083606.2 , NM_001083606.1 , XM_011518871.1 , XM_011518869.1 , NM_001083604.1 , XM_011518870.1 , NM_001083607.1 , NM_001083606.3 , NM_001083605.2 , NM_001083607.2
Accession Version	NM_001083605.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3891bp) Protein sequence SNP
Vector	pcDNA3.1⁺/C-(K)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-28
Organism	Homo sapiens(human)
Product	protein patched homolog 1 isoform S
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AB189439.1, U59464.1, AB209495.1, BX117041.1, CR744004.1 and AI417736.1. On Aug 29, 2017 this sequence version replaced NM_001083605.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## CDS exon combination :: U43148.1 [ECO:0000331] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGTTTAATC CTCAACTCAT GATACAGACC CCTAAAGAAG AAGGTGCTAA TGTCCTGACC 
ACAGAAGCGC TCCTACAACA CCTGGACTCG GCACTCCAGG CCAGCCGTGT CCATGTATAC 
ATGTACAACA GGCAGTGGAA ATTGGAACAT TTGTGTTACA AATCAGGAGA GCTTATCACA 
GAAACAGGTT ACATGGATCA GATAATAGAA TATCTTTACC CTTGTTTGAT TATTACACCT 
TTGGACTGCT TCTGGGAAGG GGCGAAATTA CAGTCTGGGA CAGCATACCT CCTAGGTAAA 
CCTCCTTTGC GGTGGACAAA CTTCGACCCT TTGGAATTCC TGGAAGAGTT AAAGAAAATA 
AACTATCAAG TGGACAGCTG GGAGGAAATG CTGAATAAGG CTGAGGTTGG TCATGGTTAC 
ATGGACCGCC CCTGCCTCAA TCCGGCCGAT CCAGACTGCC CCGCCACAGC CCCCAACAAA 
AATTCAACCA AACCTCTTGA TATGGCCCTT GTTTTGAATG GTGGATGTCA TGGCTTATCC 
AGAAAGTATA TGCACTGGCA GGAGGAGTTG ATTGTGGGTG GCACAGTCAA GAACAGCACT 
GGAAAACTCG TCAGCGCCCA TGCCCTGCAG ACCATGTTCC AGTTAATGAC TCCCAAGCAA 
ATGTACGAGC ACTTCAAGGG GTACGAGTAT GTCTCACACA TCAACTGGAA CGAGGACAAA 
GCGGCAGCCA TCCTGGAGGC CTGGCAGAGG ACATATGTGG AGGTGGTTCA TCAGAGTGTC 
GCACAGAACT CCACTCAAAA GGTGCTTTCC TTCACCACCA CGACCCTGGA CGACATCCTG 
AAATCCTTCT CTGACGTCAG TGTCATCCGC GTGGCCAGCG GCTACTTACT CATGCTCGCC 
TATGCCTGTC TAACCATGCT GCGCTGGGAC TGCTCCAAGT CCCAGGGTGC CGTGGGGCTG 
GCTGGCGTCC TGCTGGTTGC ACTGTCAGTG GCTGCAGGAC TGGGCCTGTG CTCATTGATC 
GGAATTTCCT TTAACGCTGC AACAACTCAG GTTTTGCCAT TTCTCGCTCT TGGTGTTGGT 
GTGGATGATG TTTTTCTTCT GGCCCACGCC TTCAGTGAAA CAGGACAGAA TAAAAGAATC 
CCTTTTGAGG ACAGGACCGG GGAGTGCCTG AAGCGCACAG GAGCCAGCGT GGCCCTCACG 
TCCATCAGCA ATGTCACAGC CTTCTTCATG GCCGCGTTAA TCCCAATTCC CGCTCTGCGG 
GCGTTCTCCC TCCAGGCAGC GGTAGTAGTG GTGTTCAATT TTGCCATGGT TCTGCTCATT 
TTTCCTGCAA TTCTCAGCAT GGATTTATAT CGACGCGAGG ACAGGAGACT GGATATTTTC 
TGCTGTTTTA CAAGCCCCTG CGTCAGCAGA GTGATTCAGG TTGAACCTCA GGCCTACACC 
GACACACACG ACAATACCCG CTACAGCCCC CCACCTCCCT ACAGCAGCCA CAGCTTTGCC 
CATGAAACGC AGATTACCAT GCAGTCCACT GTCCAGCTCC GCACGGAGTA CGACCCCCAC 
ACGCACGTGT ACTACACCAC CGCTGAGCCG CGCTCCGAGA TCTCTGTGCA GCCCGTCACC 
GTGACACAGG ACACCCTCAG CTGCCAGAGC CCAGAGAGCA CCAGCTCCAC AAGGGACCTG 
CTCTCCCAGT TCTCCGACTC CAGCCTCCAC TGCCTCGAGC CCCCCTGTAC GAAGTGGACA 
CTCTCATCTT TTGCTGAGAA GCACTATGCT CCTTTCCTCT TGAAACCAAA AGCCAAGGTA 
GTGGTGATCT TCCTTTTTCT GGGCTTGCTG GGGGTCAGCC TTTATGGCAC CACCCGAGTG 
AGAGACGGGC TGGACCTTAC GGACATTGTA CCTCGGGAAA CCAGAGAATA TGACTTTATT 
GCTGCACAAT TCAAATACTT TTCTTTCTAC AACATGTATA TAGTCACCCA GAAAGCAGAC 
TACCCGAATA TCCAGCACTT ACTTTACGAC CTACACAGGA GTTTCAGTAA CGTGAAGTAT 
GTCATGTTGG AAGAAAACAA ACAGCTTCCC AAAATGTGGC TGCACTACTT CAGAGACTGG 
CTTCAGGGAC TTCAGGATGC ATTTGACAGT GACTGGGAAA CCGGGAAAAT CATGCCAAAC 
AATTACAAGA ATGGATCAGA CGATGGAGTC CTTGCCTACA AACTCCTGGT GCAAACCGGC 
AGCCGCGATA AGCCCATCGA CATCAGCCAG TTGACTAAAC AGCGTCTGGT GGATGCAGAT 
GGCATCATTA ATCCCAGCGC TTTCTACATC TACCTGACGG CTTGGGTCAG CAACGACCCC 
GTCGCGTATG CTGCCTCCCA GGCCAACATC CGGCCACACC GACCAGAATG GGTCCACGAC 
AAAGCCGACT ACATGCCTGA AACAAGGCTG AGAATCCCGG CAGCAGAGCC CATCGAGTAT 
GCCCAGTTCC CTTTCTACCT CAACGGCTTG CGGGACACCT CAGACTTTGT GGAGGCAATT 
GAAAAAGTAA GGACCATCTG CAGCAACTAT ACGAGCCTGG GGCTGTCCAG TTACCCCAAC 
GGCTACCCCT TCCTCTTCTG GGAGCAGTAC ATCGGCCTCC GCCACTGGCT GCTGCTGTTC 
ATCAGCGTGG TGTTGGCCTG CACATTCCTC GTGTGCGCTG TCTTCCTTCT GAACCCCTGG 
ACGGCCGGGA TCATTGTGAT GGTCCTGGCG CTGATGACGG TCGAGCTGTT CGGCATGATG 
GGCCTCATCG GAATCAAGCT CAGTGCCGTG CCCGTGGTCA TCCTGATCGC TTCTGTTGGC 
ATAGGAGTGG AGTTCACCGT TCACGTTGCT TTGGCCTTTC TGACGGCCAT CGGCGACAAG 
AACCGCAGGG CTGTGCTTGC CCTGGAGCAC ATGTTTGCAC CCGTCCTGGA TGGCGCCGTG 
TCCACTCTGC TGGGAGTGCT GATGCTGGCG GGATCTGAGT TCGACTTCAT TGTCAGGTAT 
TTCTTTGCTG TGCTGGCGAT CCTCACCATC CTCGGCGTTC TCAATGGGCT GGTTTTGCTT 
CCCGTGCTTT TGTCTTTCTT TGGACCATAT CCTGAGGTGT CTCCAGCCAA CGGCTTGAAC 
CGCCTGCCCA CACCCTCCCC TGAGCCACCC CCCAGCGTGG TCCGCTTCGC CATGCCGCCC 
GGCCACACGC ACAGCGGGTC TGATTCCTCC GACTCGGAGT ATAGTTCCCA GACGACAGTG 
TCAGGCCTCA GCGAGGAGCT TCGGCACTAC GAGGCCCAGC AGGGCGCGGG AGGCCCTGCC 
CACCAAGTGA TCGTGGAAGC CACAGAAAAC CCCGTCTTCG CCCACTCCAC TGTGGTCCAT 
CCCGAATCCA GGCATCACCC ACCCTCGAAC CCGAGACAGC AGCCCCACCT GGACTCAGGG 
TCCCTGCCTC CCGGACGGCA AGGCCAGCAG CCCCGCAGGG ACCCCCCCAG AGAAGGCTTG 
TGGCCACCCC CCTACAGACC GCGCAGAGAC GCTTTTGAAA TTTCTACTGA AGGGCATTCT 
GGCCCTAGCA ATAGGGCCCG CTGGGGCCCT CGCGGGGCCC GTTCTCACAA CCCTCGGAAC 
CCAGCGTCCA CTGCCATGGG CAGCTCCGTG CCCGGCTACT GCCAGCCCAT CACCACTGTG 
ACGGCTTCTG CCTCCGTGAC TGTCGCCGTG CACCCGCCGC CTGTCCCTGG GCCTGGGCGG 
AACCCCCGAG GGGGACTCTG CCCAGGCTAC CCTGAGACTG ACCACGGCCT GTTTGAGGAC 
CCCCACGTGC CTTTCCACGT CCGGTGTGAG AGGAGGGATT CGAAGGTGGA AGTCATTGAG 
CTGCAGGACG TGGAATGCGA GGAGAGGCCC CGGGGAAGCA GCTCCAACTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001077074.1
CDS	343..4233
Translation	MFNPQLMIQTPKEEGANVLTTEALLQHLDSALQASRVHVYMYNRQWKLEHLCYKSGELITETGYMDQIIEYLYPCLIITPLDCFWEGAKLQSGTAYLLGKPPLRWTNFDPLEFLEELKKINYQVDSWEEMLNKAEVGHGYMDRPCLNPADPDCPATAPNKNSTKPLDMALVLNGGCHGLSRKYMHWQEELIVGGTVKNSTGKLVSAHALQTMFQLMTPKQMYEHFKGYEYVSHINWNEDKAAAILEAWQRTYVEVVHQSVAQNSTQKVLSFTTTTLDDILKSFSDVSVIRVASGYLLMLAYACLTMLRWDCSKSQGAVGLAGVLLVALSVAAGLGLCSLIGISFNAATTQVLPFLALGVGVDDVFLLAHAFSETGQNKRIPFEDRTGECLKRTGASVALTSISNVTAFFMAALIPIPALRAFSLQAAVVVVFNFAMVLLIFPAILSMDLYRREDRRLDIFCCFTSPCVSRVIQVEPQAYTDTHDNTRYSPPPPYSSHSFAHETQITMQSTVQLRTEYDPHTHVYYTTAEPRSEISVQPVTVTQDTLSCQSPESTSSTRDLLSQFSDSSLHCLEPPCTKWTLSSFAEKHYAPFLLKPKAKVVVIFLFLGLLGVSLYGTTRVRDGLDLTDIVPRETREYDFIAAQFKYFSFYNMYIVTQKADYPNIQHLLYDLHRSFSNVKYVMLEENKQLPKMWLHYFRDWLQGLQDAFDSDWETGKIMPNNYKNGSDDGVLAYKLLVQTGSRDKPIDISQLTKQRLVDADGIINPSAFYIYLTAWVSNDPVAYAASQANIRPHRPEWVHDKADYMPETRLRIPAAEPIEYAQFPFYLNGLRDTSDFVEAIEKVRTICSNYTSLGLSSYPNGYPFLFWEQYIGLRHWLLLFISVVLACTFLVCAVFLLNPWTAGIIVMVLALMTVELFGMMGLIGIKLSAVPVVILIASVGIGVEFTVHVALAFLTAIGDKNRRAVLALEHMFAPVLDGAVSTLLGVLMLAGSEFDFIVRYFFAVLAILTILGVLNGLVLLPVLLSFFGPYPEVSPANGLNRLPTPSPEPPPSVVRFAMPPGHTHSGSDSSDSEYSSQTTVSGLSEELRHYEAQQGAGGPAHQVIVEATENPVFAHSTVVHPESRHHPPSNPRQQPHLDSGSLPPGRQGQQPRRDPPREGLWPPPYRPRRDAFEISTEGHSGPSNRARWGPRGARSHNPRNPASTAMGSSVPGYCQPITTVTASASVTVAVHPPPVPGPGRNPRGGLCPGYPETDHGLFEDPHVPFHVRCERRDSKVEVIELQDVECEERPRGSSSN

Target ORF information:

RefSeq Version	NM_001083605.2
Organism	Homo sapiens(human)
Definition	Homo sapiens patched 1 (PTCH1), transcript variant 1c', mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001083605.2

ORF Insert Sequence:

ATGTTTAATC CTCAACTCAT GATACAGACC CCTAAAGAAG AAGGTGCTAA TGTCCTGACC 
ACAGAAGCGC TCCTACAACA CCTGGACTCG GCACTCCAGG CCAGCCGTGT CCATGTATAC 
ATGTACAACA GGCAGTGGAA ATTGGAACAT TTGTGTTACA AATCAGGAGA GCTTATCACA 
GAAACAGGTT ACATGGATCA GATAATAGAA TATCTTTACC CTTGTTTGAT TATTACACCT 
TTGGACTGCT TCTGGGAAGG GGCGAAATTA CAGTCTGGGA CAGCATACCT CCTAGGTAAA 
CCTCCTTTGC GGTGGACAAA CTTCGACCCT TTGGAATTCC TGGAAGAGTT AAAGAAAATA 
AACTATCAAG TGGACAGCTG GGAGGAAATG CTGAATAAGG CTGAGGTTGG TCATGGTTAC 
ATGGACCGCC CCTGCCTCAA TCCGGCCGAT CCAGACTGCC CCGCCACAGC CCCCAACAAA 
AATTCAACCA AACCTCTTGA TATGGCCCTT GTTTTGAATG GTGGATGTCA TGGCTTATCC 
AGAAAGTATA TGCACTGGCA GGAGGAGTTG ATTGTGGGTG GCACAGTCAA GAACAGCACT 
GGAAAACTCG TCAGCGCCCA TGCCCTGCAG ACCATGTTCC AGTTAATGAC TCCCAAGCAA 
ATGTACGAGC ACTTCAAGGG GTACGAGTAT GTCTCACACA TCAACTGGAA CGAGGACAAA 
GCGGCAGCCA TCCTGGAGGC CTGGCAGAGG ACATATGTGG AGGTGGTTCA TCAGAGTGTC 
GCACAGAACT CCACTCAAAA GGTGCTTTCC TTCACCACCA CGACCCTGGA CGACATCCTG 
AAATCCTTCT CTGACGTCAG TGTCATCCGC GTGGCCAGCG GCTACTTACT CATGCTCGCC 
TATGCCTGTC TAACCATGCT GCGCTGGGAC TGCTCCAAGT CCCAGGGTGC CGTGGGGCTG 
GCTGGCGTCC TGCTGGTTGC ACTGTCAGTG GCTGCAGGAC TGGGCCTGTG CTCATTGATC 
GGAATTTCCT TTAACGCTGC AACAACTCAG GTTTTGCCAT TTCTCGCTCT TGGTGTTGGT 
GTGGATGATG TTTTTCTTCT GGCCCACGCC TTCAGTGAAA CAGGACAGAA TAAAAGAATC 
CCTTTTGAGG ACAGGACCGG GGAGTGCCTG AAGCGCACAG GAGCCAGCGT GGCCCTCACG 
TCCATCAGCA ATGTCACAGC CTTCTTCATG GCCGCGTTAA TCCCAATTCC CGCTCTGCGG 
GCGTTCTCCC TCCAGGCAGC GGTAGTAGTG GTGTTCAATT TTGCCATGGT TCTGCTCATT 
TTTCCTGCAA TTCTCAGCAT GGATTTATAT CGACGCGAGG ACAGGAGACT GGATATTTTC 
TGCTGTTTTA CAAGCCCCTG CGTCAGCAGA GTGATTCAGG TTGAACCTCA GGCCTACACC 
GACACACACG ACAATACCCG CTACAGCCCC CCACCTCCCT ACAGCAGCCA CAGCTTTGCC 
CATGAAACGC AGATTACCAT GCAGTCCACT GTCCAGCTCC GCACGGAGTA CGACCCCCAC 
ACGCACGTGT ACTACACCAC CGCTGAGCCG CGCTCCGAGA TCTCTGTGCA GCCCGTCACC 
GTGACACAGG ACACCCTCAG CTGCCAGAGC CCAGAGAGCA CCAGCTCCAC AAGGGACCTG 
CTCTCCCAGT TCTCCGACTC CAGCCTCCAC TGCCTCGAGC CCCCCTGTAC GAAGTGGACA 
CTCTCATCTT TTGCTGAGAA GCACTATGCT CCTTTCCTCT TGAAACCAAA AGCCAAGGTA 
GTGGTGATCT TCCTTTTTCT GGGCTTGCTG GGGGTCAGCC TTTATGGCAC CACCCGAGTG 
AGAGACGGGC TGGACCTTAC GGACATTGTA CCTCGGGAAA CCAGAGAATA TGACTTTATT 
GCTGCACAAT TCAAATACTT TTCTTTCTAC AACATGTATA TAGTCACCCA GAAAGCAGAC 
TACCCGAATA TCCAGCACTT ACTTTACGAC CTACACAGGA GTTTCAGTAA CGTGAAGTAT 
GTCATGTTGG AAGAAAACAA ACAGCTTCCC AAAATGTGGC TGCACTACTT CAGAGACTGG 
CTTCAGGGAC TTCAGGATGC ATTTGACAGT GACTGGGAAA CCGGGAAAAT CATGCCAAAC 
AATTACAAGA ATGGATCAGA CGATGGAGTC CTTGCCTACA AACTCCTGGT GCAAACCGGC 
AGCCGCGATA AGCCCATCGA CATCAGCCAG TTGACTAAAC AGCGTCTGGT GGATGCAGAT 
GGCATCATTA ATCCCAGCGC TTTCTACATC TACCTGACGG CTTGGGTCAG CAACGACCCC 
GTCGCGTATG CTGCCTCCCA GGCCAACATC CGGCCACACC GACCAGAATG GGTCCACGAC 
AAAGCCGACT ACATGCCTGA AACAAGGCTG AGAATCCCGG CAGCAGAGCC CATCGAGTAT 
GCCCAGTTCC CTTTCTACCT CAACGGCTTG CGGGACACCT CAGACTTTGT GGAGGCAATT 
GAAAAAGTAA GGACCATCTG CAGCAACTAT ACGAGCCTGG GGCTGTCCAG TTACCCCAAC 
GGCTACCCCT TCCTCTTCTG GGAGCAGTAC ATCGGCCTCC GCCACTGGCT GCTGCTGTTC 
ATCAGCGTGG TGTTGGCCTG CACATTCCTC GTGTGCGCTG TCTTCCTTCT GAACCCCTGG 
ACGGCCGGGA TCATTGTGAT GGTCCTGGCG CTGATGACGG TCGAGCTGTT CGGCATGATG 
GGCCTCATCG GAATCAAGCT CAGTGCCGTG CCCGTGGTCA TCCTGATCGC TTCTGTTGGC 
ATAGGAGTGG AGTTCACCGT TCACGTTGCT TTGGCCTTTC TGACGGCCAT CGGCGACAAG 
AACCGCAGGG CTGTGCTTGC CCTGGAGCAC ATGTTTGCAC CCGTCCTGGA TGGCGCCGTG 
TCCACTCTGC TGGGAGTGCT GATGCTGGCG GGATCTGAGT TCGACTTCAT TGTCAGGTAT 
TTCTTTGCTG TGCTGGCGAT CCTCACCATC CTCGGCGTTC TCAATGGGCT GGTTTTGCTT 
CCCGTGCTTT TGTCTTTCTT TGGACCATAT CCTGAGGTGT CTCCAGCCAA CGGCTTGAAC 
CGCCTGCCCA CACCCTCCCC TGAGCCACCC CCCAGCGTGG TCCGCTTCGC CATGCCGCCC 
GGCCACACGC ACAGCGGGTC TGATTCCTCC GACTCGGAGT ATAGTTCCCA GACGACAGTG 
TCAGGCCTCA GCGAGGAGCT TCGGCACTAC GAGGCCCAGC AGGGCGCGGG AGGCCCTGCC 
CACCAAGTGA TCGTGGAAGC CACAGAAAAC CCCGTCTTCG CCCACTCCAC TGTGGTCCAT 
CCCGAATCCA GGCATCACCC ACCCTCGAAC CCGAGACAGC AGCCCCACCT GGACTCAGGG 
TCCCTGCCTC CCGGACGGCA AGGCCAGCAG CCCCGCAGGG ACCCCCCCAG AGAAGGCTTG 
TGGCCACCCC CCTACAGACC GCGCAGAGAC GCTTTTGAAA TTTCTACTGA AGGGCATTCT 
GGCCCTAGCA ATAGGGCCCG CTGGGGCCCT CGCGGGGCCC GTTCTCACAA CCCTCGGAAC 
CCAGCGTCCA CTGCCATGGG CAGCTCCGTG CCCGGCTACT GCCAGCCCAT CACCACTGTG 
ACGGCTTCTG CCTCCGTGAC TGTCGCCGTG CACCCGCCGC CTGTCCCTGG GCCTGGGCGG 
AACCCCCGAG GGGGACTCTG CCCAGGCTAC CCTGAGACTG ACCACGGCCT GTTTGAGGAC 
CCCCACGTGC CTTTCCACGT CCGGTGTGAG AGGAGGGATT CGAAGGTGGA AGTCATTGAG 
CTGCAGGACG TGGAATGCGA GGAGAGGCCC CGGGGAAGCA GCTCCAACTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PTCH1 ( NM_001083607.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001077076.1 Homo sapiens patched 1 (PTCH1), transcript variant 1e, mRNA.

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CloneID	OHu17265
Clone ID Related Accession (Same CDS sequence)	XM_005252102.2 , XM_011518872.1 , NM_001083605.1 , NM_001083604.2 , NM_001083606.2 , NM_001083606.1 , XM_011518871.1 , XM_011518869.1 , NM_001083604.1 , XM_011518870.1 , NM_001083607.1 , NM_001083606.3 , NM_001083605.2 , NM_001083607.2
Accession Version	NM_001083607.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3891bp) Protein sequence SNP
Vector	pcDNA3.1⁺/C-(K)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-07-25
Organism	Homo sapiens(human)
Product	protein patched homolog 1 isoform S
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AB189440.1, BF195352.1, U59464.1, AB209495.1, BX117041.1, CR744004.1 and AI417736.1. On Aug 29, 2017 this sequence version replaced NM_001083607.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## CDS exon combination :: U43148.1 [ECO:0000331] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGTTTAATC CTCAACTCAT GATACAGACC CCTAAAGAAG AAGGTGCTAA TGTCCTGACC 
ACAGAAGCGC TCCTACAACA CCTGGACTCG GCACTCCAGG CCAGCCGTGT CCATGTATAC 
ATGTACAACA GGCAGTGGAA ATTGGAACAT TTGTGTTACA AATCAGGAGA GCTTATCACA 
GAAACAGGTT ACATGGATCA GATAATAGAA TATCTTTACC CTTGTTTGAT TATTACACCT 
TTGGACTGCT TCTGGGAAGG GGCGAAATTA CAGTCTGGGA CAGCATACCT CCTAGGTAAA 
CCTCCTTTGC GGTGGACAAA CTTCGACCCT TTGGAATTCC TGGAAGAGTT AAAGAAAATA 
AACTATCAAG TGGACAGCTG GGAGGAAATG CTGAATAAGG CTGAGGTTGG TCATGGTTAC 
ATGGACCGCC CCTGCCTCAA TCCGGCCGAT CCAGACTGCC CCGCCACAGC CCCCAACAAA 
AATTCAACCA AACCTCTTGA TATGGCCCTT GTTTTGAATG GTGGATGTCA TGGCTTATCC 
AGAAAGTATA TGCACTGGCA GGAGGAGTTG ATTGTGGGTG GCACAGTCAA GAACAGCACT 
GGAAAACTCG TCAGCGCCCA TGCCCTGCAG ACCATGTTCC AGTTAATGAC TCCCAAGCAA 
ATGTACGAGC ACTTCAAGGG GTACGAGTAT GTCTCACACA TCAACTGGAA CGAGGACAAA 
GCGGCAGCCA TCCTGGAGGC CTGGCAGAGG ACATATGTGG AGGTGGTTCA TCAGAGTGTC 
GCACAGAACT CCACTCAAAA GGTGCTTTCC TTCACCACCA CGACCCTGGA CGACATCCTG 
AAATCCTTCT CTGACGTCAG TGTCATCCGC GTGGCCAGCG GCTACTTACT CATGCTCGCC 
TATGCCTGTC TAACCATGCT GCGCTGGGAC TGCTCCAAGT CCCAGGGTGC CGTGGGGCTG 
GCTGGCGTCC TGCTGGTTGC ACTGTCAGTG GCTGCAGGAC TGGGCCTGTG CTCATTGATC 
GGAATTTCCT TTAACGCTGC AACAACTCAG GTTTTGCCAT TTCTCGCTCT TGGTGTTGGT 
GTGGATGATG TTTTTCTTCT GGCCCACGCC TTCAGTGAAA CAGGACAGAA TAAAAGAATC 
CCTTTTGAGG ACAGGACCGG GGAGTGCCTG AAGCGCACAG GAGCCAGCGT GGCCCTCACG 
TCCATCAGCA ATGTCACAGC CTTCTTCATG GCCGCGTTAA TCCCAATTCC CGCTCTGCGG 
GCGTTCTCCC TCCAGGCAGC GGTAGTAGTG GTGTTCAATT TTGCCATGGT TCTGCTCATT 
TTTCCTGCAA TTCTCAGCAT GGATTTATAT CGACGCGAGG ACAGGAGACT GGATATTTTC 
TGCTGTTTTA CAAGCCCCTG CGTCAGCAGA GTGATTCAGG TTGAACCTCA GGCCTACACC 
GACACACACG ACAATACCCG CTACAGCCCC CCACCTCCCT ACAGCAGCCA CAGCTTTGCC 
CATGAAACGC AGATTACCAT GCAGTCCACT GTCCAGCTCC GCACGGAGTA CGACCCCCAC 
ACGCACGTGT ACTACACCAC CGCTGAGCCG CGCTCCGAGA TCTCTGTGCA GCCCGTCACC 
GTGACACAGG ACACCCTCAG CTGCCAGAGC CCAGAGAGCA CCAGCTCCAC AAGGGACCTG 
CTCTCCCAGT TCTCCGACTC CAGCCTCCAC TGCCTCGAGC CCCCCTGTAC GAAGTGGACA 
CTCTCATCTT TTGCTGAGAA GCACTATGCT CCTTTCCTCT TGAAACCAAA AGCCAAGGTA 
GTGGTGATCT TCCTTTTTCT GGGCTTGCTG GGGGTCAGCC TTTATGGCAC CACCCGAGTG 
AGAGACGGGC TGGACCTTAC GGACATTGTA CCTCGGGAAA CCAGAGAATA TGACTTTATT 
GCTGCACAAT TCAAATACTT TTCTTTCTAC AACATGTATA TAGTCACCCA GAAAGCAGAC 
TACCCGAATA TCCAGCACTT ACTTTACGAC CTACACAGGA GTTTCAGTAA CGTGAAGTAT 
GTCATGTTGG AAGAAAACAA ACAGCTTCCC AAAATGTGGC TGCACTACTT CAGAGACTGG 
CTTCAGGGAC TTCAGGATGC ATTTGACAGT GACTGGGAAA CCGGGAAAAT CATGCCAAAC 
AATTACAAGA ATGGATCAGA CGATGGAGTC CTTGCCTACA AACTCCTGGT GCAAACCGGC 
AGCCGCGATA AGCCCATCGA CATCAGCCAG TTGACTAAAC AGCGTCTGGT GGATGCAGAT 
GGCATCATTA ATCCCAGCGC TTTCTACATC TACCTGACGG CTTGGGTCAG CAACGACCCC 
GTCGCGTATG CTGCCTCCCA GGCCAACATC CGGCCACACC GACCAGAATG GGTCCACGAC 
AAAGCCGACT ACATGCCTGA AACAAGGCTG AGAATCCCGG CAGCAGAGCC CATCGAGTAT 
GCCCAGTTCC CTTTCTACCT CAACGGCTTG CGGGACACCT CAGACTTTGT GGAGGCAATT 
GAAAAAGTAA GGACCATCTG CAGCAACTAT ACGAGCCTGG GGCTGTCCAG TTACCCCAAC 
GGCTACCCCT TCCTCTTCTG GGAGCAGTAC ATCGGCCTCC GCCACTGGCT GCTGCTGTTC 
ATCAGCGTGG TGTTGGCCTG CACATTCCTC GTGTGCGCTG TCTTCCTTCT GAACCCCTGG 
ACGGCCGGGA TCATTGTGAT GGTCCTGGCG CTGATGACGG TCGAGCTGTT CGGCATGATG 
GGCCTCATCG GAATCAAGCT CAGTGCCGTG CCCGTGGTCA TCCTGATCGC TTCTGTTGGC 
ATAGGAGTGG AGTTCACCGT TCACGTTGCT TTGGCCTTTC TGACGGCCAT CGGCGACAAG 
AACCGCAGGG CTGTGCTTGC CCTGGAGCAC ATGTTTGCAC CCGTCCTGGA TGGCGCCGTG 
TCCACTCTGC TGGGAGTGCT GATGCTGGCG GGATCTGAGT TCGACTTCAT TGTCAGGTAT 
TTCTTTGCTG TGCTGGCGAT CCTCACCATC CTCGGCGTTC TCAATGGGCT GGTTTTGCTT 
CCCGTGCTTT TGTCTTTCTT TGGACCATAT CCTGAGGTGT CTCCAGCCAA CGGCTTGAAC 
CGCCTGCCCA CACCCTCCCC TGAGCCACCC CCCAGCGTGG TCCGCTTCGC CATGCCGCCC 
GGCCACACGC ACAGCGGGTC TGATTCCTCC GACTCGGAGT ATAGTTCCCA GACGACAGTG 
TCAGGCCTCA GCGAGGAGCT TCGGCACTAC GAGGCCCAGC AGGGCGCGGG AGGCCCTGCC 
CACCAAGTGA TCGTGGAAGC CACAGAAAAC CCCGTCTTCG CCCACTCCAC TGTGGTCCAT 
CCCGAATCCA GGCATCACCC ACCCTCGAAC CCGAGACAGC AGCCCCACCT GGACTCAGGG 
TCCCTGCCTC CCGGACGGCA AGGCCAGCAG CCCCGCAGGG ACCCCCCCAG AGAAGGCTTG 
TGGCCACCCC CCTACAGACC GCGCAGAGAC GCTTTTGAAA TTTCTACTGA AGGGCATTCT 
GGCCCTAGCA ATAGGGCCCG CTGGGGCCCT CGCGGGGCCC GTTCTCACAA CCCTCGGAAC 
CCAGCGTCCA CTGCCATGGG CAGCTCCGTG CCCGGCTACT GCCAGCCCAT CACCACTGTG 
ACGGCTTCTG CCTCCGTGAC TGTCGCCGTG CACCCGCCGC CTGTCCCTGG GCCTGGGCGG 
AACCCCCGAG GGGGACTCTG CCCAGGCTAC CCTGAGACTG ACCACGGCCT GTTTGAGGAC 
CCCCACGTGC CTTTCCACGT CCGGTGTGAG AGGAGGGATT CGAAGGTGGA AGTCATTGAG 
CTGCAGGACG TGGAATGCGA GGAGAGGCCC CGGGGAAGCA GCTCCAACTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001077076.1
CDS	516..4406
Translation	MFNPQLMIQTPKEEGANVLTTEALLQHLDSALQASRVHVYMYNRQWKLEHLCYKSGELITETGYMDQIIEYLYPCLIITPLDCFWEGAKLQSGTAYLLGKPPLRWTNFDPLEFLEELKKINYQVDSWEEMLNKAEVGHGYMDRPCLNPADPDCPATAPNKNSTKPLDMALVLNGGCHGLSRKYMHWQEELIVGGTVKNSTGKLVSAHALQTMFQLMTPKQMYEHFKGYEYVSHINWNEDKAAAILEAWQRTYVEVVHQSVAQNSTQKVLSFTTTTLDDILKSFSDVSVIRVASGYLLMLAYACLTMLRWDCSKSQGAVGLAGVLLVALSVAAGLGLCSLIGISFNAATTQVLPFLALGVGVDDVFLLAHAFSETGQNKRIPFEDRTGECLKRTGASVALTSISNVTAFFMAALIPIPALRAFSLQAAVVVVFNFAMVLLIFPAILSMDLYRREDRRLDIFCCFTSPCVSRVIQVEPQAYTDTHDNTRYSPPPPYSSHSFAHETQITMQSTVQLRTEYDPHTHVYYTTAEPRSEISVQPVTVTQDTLSCQSPESTSSTRDLLSQFSDSSLHCLEPPCTKWTLSSFAEKHYAPFLLKPKAKVVVIFLFLGLLGVSLYGTTRVRDGLDLTDIVPRETREYDFIAAQFKYFSFYNMYIVTQKADYPNIQHLLYDLHRSFSNVKYVMLEENKQLPKMWLHYFRDWLQGLQDAFDSDWETGKIMPNNYKNGSDDGVLAYKLLVQTGSRDKPIDISQLTKQRLVDADGIINPSAFYIYLTAWVSNDPVAYAASQANIRPHRPEWVHDKADYMPETRLRIPAAEPIEYAQFPFYLNGLRDTSDFVEAIEKVRTICSNYTSLGLSSYPNGYPFLFWEQYIGLRHWLLLFISVVLACTFLVCAVFLLNPWTAGIIVMVLALMTVELFGMMGLIGIKLSAVPVVILIASVGIGVEFTVHVALAFLTAIGDKNRRAVLALEHMFAPVLDGAVSTLLGVLMLAGSEFDFIVRYFFAVLAILTILGVLNGLVLLPVLLSFFGPYPEVSPANGLNRLPTPSPEPPPSVVRFAMPPGHTHSGSDSSDSEYSSQTTVSGLSEELRHYEAQQGAGGPAHQVIVEATENPVFAHSTVVHPESRHHPPSNPRQQPHLDSGSLPPGRQGQQPRRDPPREGLWPPPYRPRRDAFEISTEGHSGPSNRARWGPRGARSHNPRNPASTAMGSSVPGYCQPITTVTASASVTVAVHPPPVPGPGRNPRGGLCPGYPETDHGLFEDPHVPFHVRCERRDSKVEVIELQDVECEERPRGSSSN

Target ORF information:

RefSeq Version	NM_001083607.2
Organism	Homo sapiens(human)
Definition	Homo sapiens patched 1 (PTCH1), transcript variant 1e, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001083607.2

ORF Insert Sequence:

ATGTTTAATC CTCAACTCAT GATACAGACC CCTAAAGAAG AAGGTGCTAA TGTCCTGACC 
ACAGAAGCGC TCCTACAACA CCTGGACTCG GCACTCCAGG CCAGCCGTGT CCATGTATAC 
ATGTACAACA GGCAGTGGAA ATTGGAACAT TTGTGTTACA AATCAGGAGA GCTTATCACA 
GAAACAGGTT ACATGGATCA GATAATAGAA TATCTTTACC CTTGTTTGAT TATTACACCT 
TTGGACTGCT TCTGGGAAGG GGCGAAATTA CAGTCTGGGA CAGCATACCT CCTAGGTAAA 
CCTCCTTTGC GGTGGACAAA CTTCGACCCT TTGGAATTCC TGGAAGAGTT AAAGAAAATA 
AACTATCAAG TGGACAGCTG GGAGGAAATG CTGAATAAGG CTGAGGTTGG TCATGGTTAC 
ATGGACCGCC CCTGCCTCAA TCCGGCCGAT CCAGACTGCC CCGCCACAGC CCCCAACAAA 
AATTCAACCA AACCTCTTGA TATGGCCCTT GTTTTGAATG GTGGATGTCA TGGCTTATCC 
AGAAAGTATA TGCACTGGCA GGAGGAGTTG ATTGTGGGTG GCACAGTCAA GAACAGCACT 
GGAAAACTCG TCAGCGCCCA TGCCCTGCAG ACCATGTTCC AGTTAATGAC TCCCAAGCAA 
ATGTACGAGC ACTTCAAGGG GTACGAGTAT GTCTCACACA TCAACTGGAA CGAGGACAAA 
GCGGCAGCCA TCCTGGAGGC CTGGCAGAGG ACATATGTGG AGGTGGTTCA TCAGAGTGTC 
GCACAGAACT CCACTCAAAA GGTGCTTTCC TTCACCACCA CGACCCTGGA CGACATCCTG 
AAATCCTTCT CTGACGTCAG TGTCATCCGC GTGGCCAGCG GCTACTTACT CATGCTCGCC 
TATGCCTGTC TAACCATGCT GCGCTGGGAC TGCTCCAAGT CCCAGGGTGC CGTGGGGCTG 
GCTGGCGTCC TGCTGGTTGC ACTGTCAGTG GCTGCAGGAC TGGGCCTGTG CTCATTGATC 
GGAATTTCCT TTAACGCTGC AACAACTCAG GTTTTGCCAT TTCTCGCTCT TGGTGTTGGT 
GTGGATGATG TTTTTCTTCT GGCCCACGCC TTCAGTGAAA CAGGACAGAA TAAAAGAATC 
CCTTTTGAGG ACAGGACCGG GGAGTGCCTG AAGCGCACAG GAGCCAGCGT GGCCCTCACG 
TCCATCAGCA ATGTCACAGC CTTCTTCATG GCCGCGTTAA TCCCAATTCC CGCTCTGCGG 
GCGTTCTCCC TCCAGGCAGC GGTAGTAGTG GTGTTCAATT TTGCCATGGT TCTGCTCATT 
TTTCCTGCAA TTCTCAGCAT GGATTTATAT CGACGCGAGG ACAGGAGACT GGATATTTTC 
TGCTGTTTTA CAAGCCCCTG CGTCAGCAGA GTGATTCAGG TTGAACCTCA GGCCTACACC 
GACACACACG ACAATACCCG CTACAGCCCC CCACCTCCCT ACAGCAGCCA CAGCTTTGCC 
CATGAAACGC AGATTACCAT GCAGTCCACT GTCCAGCTCC GCACGGAGTA CGACCCCCAC 
ACGCACGTGT ACTACACCAC CGCTGAGCCG CGCTCCGAGA TCTCTGTGCA GCCCGTCACC 
GTGACACAGG ACACCCTCAG CTGCCAGAGC CCAGAGAGCA CCAGCTCCAC AAGGGACCTG 
CTCTCCCAGT TCTCCGACTC CAGCCTCCAC TGCCTCGAGC CCCCCTGTAC GAAGTGGACA 
CTCTCATCTT TTGCTGAGAA GCACTATGCT CCTTTCCTCT TGAAACCAAA AGCCAAGGTA 
GTGGTGATCT TCCTTTTTCT GGGCTTGCTG GGGGTCAGCC TTTATGGCAC CACCCGAGTG 
AGAGACGGGC TGGACCTTAC GGACATTGTA CCTCGGGAAA CCAGAGAATA TGACTTTATT 
GCTGCACAAT TCAAATACTT TTCTTTCTAC AACATGTATA TAGTCACCCA GAAAGCAGAC 
TACCCGAATA TCCAGCACTT ACTTTACGAC CTACACAGGA GTTTCAGTAA CGTGAAGTAT 
GTCATGTTGG AAGAAAACAA ACAGCTTCCC AAAATGTGGC TGCACTACTT CAGAGACTGG 
CTTCAGGGAC TTCAGGATGC ATTTGACAGT GACTGGGAAA CCGGGAAAAT CATGCCAAAC 
AATTACAAGA ATGGATCAGA CGATGGAGTC CTTGCCTACA AACTCCTGGT GCAAACCGGC 
AGCCGCGATA AGCCCATCGA CATCAGCCAG TTGACTAAAC AGCGTCTGGT GGATGCAGAT 
GGCATCATTA ATCCCAGCGC TTTCTACATC TACCTGACGG CTTGGGTCAG CAACGACCCC 
GTCGCGTATG CTGCCTCCCA GGCCAACATC CGGCCACACC GACCAGAATG GGTCCACGAC 
AAAGCCGACT ACATGCCTGA AACAAGGCTG AGAATCCCGG CAGCAGAGCC CATCGAGTAT 
GCCCAGTTCC CTTTCTACCT CAACGGCTTG CGGGACACCT CAGACTTTGT GGAGGCAATT 
GAAAAAGTAA GGACCATCTG CAGCAACTAT ACGAGCCTGG GGCTGTCCAG TTACCCCAAC 
GGCTACCCCT TCCTCTTCTG GGAGCAGTAC ATCGGCCTCC GCCACTGGCT GCTGCTGTTC 
ATCAGCGTGG TGTTGGCCTG CACATTCCTC GTGTGCGCTG TCTTCCTTCT GAACCCCTGG 
ACGGCCGGGA TCATTGTGAT GGTCCTGGCG CTGATGACGG TCGAGCTGTT CGGCATGATG 
GGCCTCATCG GAATCAAGCT CAGTGCCGTG CCCGTGGTCA TCCTGATCGC TTCTGTTGGC 
ATAGGAGTGG AGTTCACCGT TCACGTTGCT TTGGCCTTTC TGACGGCCAT CGGCGACAAG 
AACCGCAGGG CTGTGCTTGC CCTGGAGCAC ATGTTTGCAC CCGTCCTGGA TGGCGCCGTG 
TCCACTCTGC TGGGAGTGCT GATGCTGGCG GGATCTGAGT TCGACTTCAT TGTCAGGTAT 
TTCTTTGCTG TGCTGGCGAT CCTCACCATC CTCGGCGTTC TCAATGGGCT GGTTTTGCTT 
CCCGTGCTTT TGTCTTTCTT TGGACCATAT CCTGAGGTGT CTCCAGCCAA CGGCTTGAAC 
CGCCTGCCCA CACCCTCCCC TGAGCCACCC CCCAGCGTGG TCCGCTTCGC CATGCCGCCC 
GGCCACACGC ACAGCGGGTC TGATTCCTCC GACTCGGAGT ATAGTTCCCA GACGACAGTG 
TCAGGCCTCA GCGAGGAGCT TCGGCACTAC GAGGCCCAGC AGGGCGCGGG AGGCCCTGCC 
CACCAAGTGA TCGTGGAAGC CACAGAAAAC CCCGTCTTCG CCCACTCCAC TGTGGTCCAT 
CCCGAATCCA GGCATCACCC ACCCTCGAAC CCGAGACAGC AGCCCCACCT GGACTCAGGG 
TCCCTGCCTC CCGGACGGCA AGGCCAGCAG CCCCGCAGGG ACCCCCCCAG AGAAGGCTTG 
TGGCCACCCC CCTACAGACC GCGCAGAGAC GCTTTTGAAA TTTCTACTGA AGGGCATTCT 
GGCCCTAGCA ATAGGGCCCG CTGGGGCCCT CGCGGGGCCC GTTCTCACAA CCCTCGGAAC 
CCAGCGTCCA CTGCCATGGG CAGCTCCGTG CCCGGCTACT GCCAGCCCAT CACCACTGTG 
ACGGCTTCTG CCTCCGTGAC TGTCGCCGTG CACCCGCCGC CTGTCCCTGG GCCTGGGCGG 
AACCCCCGAG GGGGACTCTG CCCAGGCTAC CCTGAGACTG ACCACGGCCT GTTTGAGGAC 
CCCCACGTGC CTTTCCACGT CCGGTGTGAG AGGAGGGATT CGAAGGTGGA AGTCATTGAG 
CTGCAGGACG TGGAATGCGA GGAGAGGCCC CGGGGAAGCA GCTCCAACTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PTCH1 ( NM_000264.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_000255.2 Homo sapiens patched 1 (PTCH1), transcript variant 1b, mRNA.

Price & Availability↑

CloneID	OHu18800
Clone ID Related Accession (Same CDS sequence)	NM_000264.4 , NM_000264.3 , NM_000264.5
Accession Version	NM_000264.4	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4344bp) Protein sequence SNP
Vector	pcDNA3.1⁺/C-(K)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-06-10
Organism	Homo sapiens(human)
Product	protein patched homolog 1 isoform L
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AI494442.1, U59464.1, DB093644.1, AB209495.1, BX117041.1, CR744004.1 and AI417736.1. On Aug 29, 2017 this sequence version replaced NM_000264.3. Transcript Variant: This variant (1b) encodes the longest isoform (L). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## CDS exon combination :: U59464.1 [ECO:0000331] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGGCCTCGG CTGGTAACGC CGCCGAGCCC CAGGACCGCG GCGGCGGCGG CAGCGGCTGT 
ATCGGTGCCC CGGGACGGCC GGCTGGAGGC GGGAGGCGCA GACGGACGGG GGGGCTGCGC 
CGTGCTGCCG CGCCGGACCG GGACTATCTG CACCGGCCCA GCTACTGCGA CGCCGCCTTC 
GCTCTGGAGC AGATTTCCAA GGGGAAGGCT ACTGGCCGGA AAGCGCCGCT GTGGCTGAGA 
GCGAAGTTTC AGAGACTCTT ATTTAAACTG GGTTGTTACA TTCAAAAAAA CTGCGGCAAG 
TTCTTGGTTG TGGGCCTCCT CATATTTGGG GCCTTCGCGG TGGGATTAAA AGCAGCGAAC 
CTCGAGACCA ACGTGGAGGA GCTGTGGGTG GAAGTTGGAG GACGAGTAAG TCGTGAATTA 
AATTATACTC GCCAGAAGAT TGGAGAAGAG GCTATGTTTA ATCCTCAACT CATGATACAG 
ACCCCTAAAG AAGAAGGTGC TAATGTCCTG ACCACAGAAG CGCTCCTACA ACACCTGGAC 
TCGGCACTCC AGGCCAGCCG TGTCCATGTA TACATGTACA ACAGGCAGTG GAAATTGGAA 
CATTTGTGTT ACAAATCAGG AGAGCTTATC ACAGAAACAG GTTACATGGA TCAGATAATA 
GAATATCTTT ACCCTTGTTT GATTATTACA CCTTTGGACT GCTTCTGGGA AGGGGCGAAA 
TTACAGTCTG GGACAGCATA CCTCCTAGGT AAACCTCCTT TGCGGTGGAC AAACTTCGAC 
CCTTTGGAAT TCCTGGAAGA GTTAAAGAAA ATAAACTATC AAGTGGACAG CTGGGAGGAA 
ATGCTGAATA AGGCTGAGGT TGGTCATGGT TACATGGACC GCCCCTGCCT CAATCCGGCC 
GATCCAGACT GCCCCGCCAC AGCCCCCAAC AAAAATTCAA CCAAACCTCT TGATATGGCC 
CTTGTTTTGA ATGGTGGATG TCATGGCTTA TCCAGAAAGT ATATGCACTG GCAGGAGGAG 
TTGATTGTGG GTGGCACAGT CAAGAACAGC ACTGGAAAAC TCGTCAGCGC CCATGCCCTG 
CAGACCATGT TCCAGTTAAT GACTCCCAAG CAAATGTACG AGCACTTCAA GGGGTACGAG 
TATGTCTCAC ACATCAACTG GAACGAGGAC AAAGCGGCAG CCATCCTGGA GGCCTGGCAG 
AGGACATATG TGGAGGTGGT TCATCAGAGT GTCGCACAGA ACTCCACTCA AAAGGTGCTT 
TCCTTCACCA CCACGACCCT GGACGACATC CTGAAATCCT TCTCTGACGT CAGTGTCATC 
CGCGTGGCCA GCGGCTACTT ACTCATGCTC GCCTATGCCT GTCTAACCAT GCTGCGCTGG 
GACTGCTCCA AGTCCCAGGG TGCCGTGGGG CTGGCTGGCG TCCTGCTGGT TGCACTGTCA 
GTGGCTGCAG GACTGGGCCT GTGCTCATTG ATCGGAATTT CCTTTAACGC TGCAACAACT 
CAGGTTTTGC CATTTCTCGC TCTTGGTGTT GGTGTGGATG ATGTTTTTCT TCTGGCCCAC 
GCCTTCAGTG AAACAGGACA GAATAAAAGA ATCCCTTTTG AGGACAGGAC CGGGGAGTGC 
CTGAAGCGCA CAGGAGCCAG CGTGGCCCTC ACGTCCATCA GCAATGTCAC AGCCTTCTTC 
ATGGCCGCGT TAATCCCAAT TCCCGCTCTG CGGGCGTTCT CCCTCCAGGC AGCGGTAGTA 
GTGGTGTTCA ATTTTGCCAT GGTTCTGCTC ATTTTTCCTG CAATTCTCAG CATGGATTTA 
TATCGACGCG AGGACAGGAG ACTGGATATT TTCTGCTGTT TTACAAGCCC CTGCGTCAGC 
AGAGTGATTC AGGTTGAACC TCAGGCCTAC ACCGACACAC ACGACAATAC CCGCTACAGC 
CCCCCACCTC CCTACAGCAG CCACAGCTTT GCCCATGAAA CGCAGATTAC CATGCAGTCC 
ACTGTCCAGC TCCGCACGGA GTACGACCCC CACACGCACG TGTACTACAC CACCGCTGAG 
CCGCGCTCCG AGATCTCTGT GCAGCCCGTC ACCGTGACAC AGGACACCCT CAGCTGCCAG 
AGCCCAGAGA GCACCAGCTC CACAAGGGAC CTGCTCTCCC AGTTCTCCGA CTCCAGCCTC 
CACTGCCTCG AGCCCCCCTG TACGAAGTGG ACACTCTCAT CTTTTGCTGA GAAGCACTAT 
GCTCCTTTCC TCTTGAAACC AAAAGCCAAG GTAGTGGTGA TCTTCCTTTT TCTGGGCTTG 
CTGGGGGTCA GCCTTTATGG CACCACCCGA GTGAGAGACG GGCTGGACCT TACGGACATT 
GTACCTCGGG AAACCAGAGA ATATGACTTT ATTGCTGCAC AATTCAAATA CTTTTCTTTC 
TACAACATGT ATATAGTCAC CCAGAAAGCA GACTACCCGA ATATCCAGCA CTTACTTTAC 
GACCTACACA GGAGTTTCAG TAACGTGAAG TATGTCATGT TGGAAGAAAA CAAACAGCTT 
CCCAAAATGT GGCTGCACTA CTTCAGAGAC TGGCTTCAGG GACTTCAGGA TGCATTTGAC 
AGTGACTGGG AAACCGGGAA AATCATGCCA AACAATTACA AGAATGGATC AGACGATGGA 
GTCCTTGCCT ACAAACTCCT GGTGCAAACC GGCAGCCGCG ATAAGCCCAT CGACATCAGC 
CAGTTGACTA AACAGCGTCT GGTGGATGCA GATGGCATCA TTAATCCCAG CGCTTTCTAC 
ATCTACCTGA CGGCTTGGGT CAGCAACGAC CCCGTCGCGT ATGCTGCCTC CCAGGCCAAC 
ATCCGGCCAC ACCGACCAGA ATGGGTCCAC GACAAAGCCG ACTACATGCC TGAAACAAGG 
CTGAGAATCC CGGCAGCAGA GCCCATCGAG TATGCCCAGT TCCCTTTCTA CCTCAACGGC 
TTGCGGGACA CCTCAGACTT TGTGGAGGCA ATTGAAAAAG TAAGGACCAT CTGCAGCAAC 
TATACGAGCC TGGGGCTGTC CAGTTACCCC AACGGCTACC CCTTCCTCTT CTGGGAGCAG 
TACATCGGCC TCCGCCACTG GCTGCTGCTG TTCATCAGCG TGGTGTTGGC CTGCACATTC 
CTCGTGTGCG CTGTCTTCCT TCTGAACCCC TGGACGGCCG GGATCATTGT GATGGTCCTG 
GCGCTGATGA CGGTCGAGCT GTTCGGCATG ATGGGCCTCA TCGGAATCAA GCTCAGTGCC 
GTGCCCGTGG TCATCCTGAT CGCTTCTGTT GGCATAGGAG TGGAGTTCAC CGTTCACGTT 
GCTTTGGCCT TTCTGACGGC CATCGGCGAC AAGAACCGCA GGGCTGTGCT TGCCCTGGAG 
CACATGTTTG CACCCGTCCT GGATGGCGCC GTGTCCACTC TGCTGGGAGT GCTGATGCTG 
GCGGGATCTG AGTTCGACTT CATTGTCAGG TATTTCTTTG CTGTGCTGGC GATCCTCACC 
ATCCTCGGCG TTCTCAATGG GCTGGTTTTG CTTCCCGTGC TTTTGTCTTT CTTTGGACCA 
TATCCTGAGG TGTCTCCAGC CAACGGCTTG AACCGCCTGC CCACACCCTC CCCTGAGCCA 
CCCCCCAGCG TGGTCCGCTT CGCCATGCCG CCCGGCCACA CGCACAGCGG GTCTGATTCC 
TCCGACTCGG AGTATAGTTC CCAGACGACA GTGTCAGGCC TCAGCGAGGA GCTTCGGCAC 
TACGAGGCCC AGCAGGGCGC GGGAGGCCCT GCCCACCAAG TGATCGTGGA AGCCACAGAA 
AACCCCGTCT TCGCCCACTC CACTGTGGTC CATCCCGAAT CCAGGCATCA CCCACCCTCG 
AACCCGAGAC AGCAGCCCCA CCTGGACTCA GGGTCCCTGC CTCCCGGACG GCAAGGCCAG 
CAGCCCCGCA GGGACCCCCC CAGAGAAGGC TTGTGGCCAC CCCCCTACAG ACCGCGCAGA 
GACGCTTTTG AAATTTCTAC TGAAGGGCAT TCTGGCCCTA GCAATAGGGC CCGCTGGGGC 
CCTCGCGGGG CCCGTTCTCA CAACCCTCGG AACCCAGCGT CCACTGCCAT GGGCAGCTCC 
GTGCCCGGCT ACTGCCAGCC CATCACCACT GTGACGGCTT CTGCCTCCGT GACTGTCGCC 
GTGCACCCGC CGCCTGTCCC TGGGCCTGGG CGGAACCCCC GAGGGGGACT CTGCCCAGGC 
TACCCTGAGA CTGACCACGG CCTGTTTGAG GACCCCCACG TGCCTTTCCA CGTCCGGTGT 
GAGAGGAGGG ATTCGAAGGT GGAAGTCATT GAGCTGCAGG ACGTGGAATG CGAGGAGAGG 
CCCCGGGGAA GCAGCTCCAA CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_000255.2
CDS	189..4532
Translation	MASAGNAAEPQDRGGGGSGCIGAPGRPAGGGRRRRTGGLRRAAAPDRDYLHRPSYCDAAFALEQISKGKATGRKAPLWLRAKFQRLLFKLGCYIQKNCGKFLVVGLLIFGAFAVGLKAANLETNVEELWVEVGGRVSRELNYTRQKIGEEAMFNPQLMIQTPKEEGANVLTTEALLQHLDSALQASRVHVYMYNRQWKLEHLCYKSGELITETGYMDQIIEYLYPCLIITPLDCFWEGAKLQSGTAYLLGKPPLRWTNFDPLEFLEELKKINYQVDSWEEMLNKAEVGHGYMDRPCLNPADPDCPATAPNKNSTKPLDMALVLNGGCHGLSRKYMHWQEELIVGGTVKNSTGKLVSAHALQTMFQLMTPKQMYEHFKGYEYVSHINWNEDKAAAILEAWQRTYVEVVHQSVAQNSTQKVLSFTTTTLDDILKSFSDVSVIRVASGYLLMLAYACLTMLRWDCSKSQGAVGLAGVLLVALSVAAGLGLCSLIGISFNAATTQVLPFLALGVGVDDVFLLAHAFSETGQNKRIPFEDRTGECLKRTGASVALTSISNVTAFFMAALIPIPALRAFSLQAAVVVVFNFAMVLLIFPAILSMDLYRREDRRLDIFCCFTSPCVSRVIQVEPQAYTDTHDNTRYSPPPPYSSHSFAHETQITMQSTVQLRTEYDPHTHVYYTTAEPRSEISVQPVTVTQDTLSCQSPESTSSTRDLLSQFSDSSLHCLEPPCTKWTLSSFAEKHYAPFLLKPKAKVVVIFLFLGLLGVSLYGTTRVRDGLDLTDIVPRETREYDFIAAQFKYFSFYNMYIVTQKADYPNIQHLLYDLHRSFSNVKYVMLEENKQLPKMWLHYFRDWLQGLQDAFDSDWETGKIMPNNYKNGSDDGVLAYKLLVQTGSRDKPIDISQLTKQRLVDADGIINPSAFYIYLTAWVSNDPVAYAASQANIRPHRPEWVHDKADYMPETRLRIPAAEPIEYAQFPFYLNGLRDTSDFVEAIEKVRTICSNYTSLGLSSYPNGYPFLFWEQYIGLRHWLLLFISVVLACTFLVCAVFLLNPWTAGIIVMVLALMTVELFGMMGLIGIKLSAVPVVILIASVGIGVEFTVHVALAFLTAIGDKNRRAVLALEHMFAPVLDGAVSTLLGVLMLAGSEFDFIVRYFFAVLAILTILGVLNGLVLLPVLLSFFGPYPEVSPANGLNRLPTPSPEPPPSVVRFAMPPGHTHSGSDSSDSEYSSQTTVSGLSEELRHYEAQQGAGGPAHQVIVEATENPVFAHSTVVHPESRHHPPSNPRQQPHLDSGSLPPGRQGQQPRRDPPREGLWPPPYRPRRDAFEISTEGHSGPSNRARWGPRGARSHNPRNPASTAMGSSVPGYCQPITTVTASASVTVAVHPPPVPGPGRNPRGGLCPGYPETDHGLFEDPHVPFHVRCERRDSKVEVIELQDVECEERPRGSSSN

Target ORF information:

RefSeq Version	NM_000264.4
Organism	Homo sapiens(human)
Definition	Homo sapiens patched 1 (PTCH1), transcript variant 1b, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_000264.4

ORF Insert Sequence:

ATGGCCTCGG CTGGTAACGC CGCCGAGCCC CAGGACCGCG GCGGCGGCGG CAGCGGCTGT 
ATCGGTGCCC CGGGACGGCC GGCTGGAGGC GGGAGGCGCA GACGGACGGG GGGGCTGCGC 
CGTGCTGCCG CGCCGGACCG GGACTATCTG CACCGGCCCA GCTACTGCGA CGCCGCCTTC 
GCTCTGGAGC AGATTTCCAA GGGGAAGGCT ACTGGCCGGA AAGCGCCGCT GTGGCTGAGA 
GCGAAGTTTC AGAGACTCTT ATTTAAACTG GGTTGTTACA TTCAAAAAAA CTGCGGCAAG 
TTCTTGGTTG TGGGCCTCCT CATATTTGGG GCCTTCGCGG TGGGATTAAA AGCAGCGAAC 
CTCGAGACCA ACGTGGAGGA GCTGTGGGTG GAAGTTGGAG GACGAGTAAG TCGTGAATTA 
AATTATACTC GCCAGAAGAT TGGAGAAGAG GCTATGTTTA ATCCTCAACT CATGATACAG 
ACCCCTAAAG AAGAAGGTGC TAATGTCCTG ACCACAGAAG CGCTCCTACA ACACCTGGAC 
TCGGCACTCC AGGCCAGCCG TGTCCATGTA TACATGTACA ACAGGCAGTG GAAATTGGAA 
CATTTGTGTT ACAAATCAGG AGAGCTTATC ACAGAAACAG GTTACATGGA TCAGATAATA 
GAATATCTTT ACCCTTGTTT GATTATTACA CCTTTGGACT GCTTCTGGGA AGGGGCGAAA 
TTACAGTCTG GGACAGCATA CCTCCTAGGT AAACCTCCTT TGCGGTGGAC AAACTTCGAC 
CCTTTGGAAT TCCTGGAAGA GTTAAAGAAA ATAAACTATC AAGTGGACAG CTGGGAGGAA 
ATGCTGAATA AGGCTGAGGT TGGTCATGGT TACATGGACC GCCCCTGCCT CAATCCGGCC 
GATCCAGACT GCCCCGCCAC AGCCCCCAAC AAAAATTCAA CCAAACCTCT TGATATGGCC 
CTTGTTTTGA ATGGTGGATG TCATGGCTTA TCCAGAAAGT ATATGCACTG GCAGGAGGAG 
TTGATTGTGG GTGGCACAGT CAAGAACAGC ACTGGAAAAC TCGTCAGCGC CCATGCCCTG 
CAGACCATGT TCCAGTTAAT GACTCCCAAG CAAATGTACG AGCACTTCAA GGGGTACGAG 
TATGTCTCAC ACATCAACTG GAACGAGGAC AAAGCGGCAG CCATCCTGGA GGCCTGGCAG 
AGGACATATG TGGAGGTGGT TCATCAGAGT GTCGCACAGA ACTCCACTCA AAAGGTGCTT 
TCCTTCACCA CCACGACCCT GGACGACATC CTGAAATCCT TCTCTGACGT CAGTGTCATC 
CGCGTGGCCA GCGGCTACTT ACTCATGCTC GCCTATGCCT GTCTAACCAT GCTGCGCTGG 
GACTGCTCCA AGTCCCAGGG TGCCGTGGGG CTGGCTGGCG TCCTGCTGGT TGCACTGTCA 
GTGGCTGCAG GACTGGGCCT GTGCTCATTG ATCGGAATTT CCTTTAACGC TGCAACAACT 
CAGGTTTTGC CATTTCTCGC TCTTGGTGTT GGTGTGGATG ATGTTTTTCT TCTGGCCCAC 
GCCTTCAGTG AAACAGGACA GAATAAAAGA ATCCCTTTTG AGGACAGGAC CGGGGAGTGC 
CTGAAGCGCA CAGGAGCCAG CGTGGCCCTC ACGTCCATCA GCAATGTCAC AGCCTTCTTC 
ATGGCCGCGT TAATCCCAAT TCCCGCTCTG CGGGCGTTCT CCCTCCAGGC AGCGGTAGTA 
GTGGTGTTCA ATTTTGCCAT GGTTCTGCTC ATTTTTCCTG CAATTCTCAG CATGGATTTA 
TATCGACGCG AGGACAGGAG ACTGGATATT TTCTGCTGTT TTACAAGCCC CTGCGTCAGC 
AGAGTGATTC AGGTTGAACC TCAGGCCTAC ACCGACACAC ACGACAATAC CCGCTACAGC 
CCCCCACCTC CCTACAGCAG CCACAGCTTT GCCCATGAAA CGCAGATTAC CATGCAGTCC 
ACTGTCCAGC TCCGCACGGA GTACGACCCC CACACGCACG TGTACTACAC CACCGCTGAG 
CCGCGCTCCG AGATCTCTGT GCAGCCCGTC ACCGTGACAC AGGACACCCT CAGCTGCCAG 
AGCCCAGAGA GCACCAGCTC CACAAGGGAC CTGCTCTCCC AGTTCTCCGA CTCCAGCCTC 
CACTGCCTCG AGCCCCCCTG TACGAAGTGG ACACTCTCAT CTTTTGCTGA GAAGCACTAT 
GCTCCTTTCC TCTTGAAACC AAAAGCCAAG GTAGTGGTGA TCTTCCTTTT TCTGGGCTTG 
CTGGGGGTCA GCCTTTATGG CACCACCCGA GTGAGAGACG GGCTGGACCT TACGGACATT 
GTACCTCGGG AAACCAGAGA ATATGACTTT ATTGCTGCAC AATTCAAATA CTTTTCTTTC 
TACAACATGT ATATAGTCAC CCAGAAAGCA GACTACCCGA ATATCCAGCA CTTACTTTAC 
GACCTACACA GGAGTTTCAG TAACGTGAAG TATGTCATGT TGGAAGAAAA CAAACAGCTT 
CCCAAAATGT GGCTGCACTA CTTCAGAGAC TGGCTTCAGG GACTTCAGGA TGCATTTGAC 
AGTGACTGGG AAACCGGGAA AATCATGCCA AACAATTACA AGAATGGATC AGACGATGGA 
GTCCTTGCCT ACAAACTCCT GGTGCAAACC GGCAGCCGCG ATAAGCCCAT CGACATCAGC 
CAGTTGACTA AACAGCGTCT GGTGGATGCA GATGGCATCA TTAATCCCAG CGCTTTCTAC 
ATCTACCTGA CGGCTTGGGT CAGCAACGAC CCCGTCGCGT ATGCTGCCTC CCAGGCCAAC 
ATCCGGCCAC ACCGACCAGA ATGGGTCCAC GACAAAGCCG ACTACATGCC TGAAACAAGG 
CTGAGAATCC CGGCAGCAGA GCCCATCGAG TATGCCCAGT TCCCTTTCTA CCTCAACGGC 
TTGCGGGACA CCTCAGACTT TGTGGAGGCA ATTGAAAAAG TAAGGACCAT CTGCAGCAAC 
TATACGAGCC TGGGGCTGTC CAGTTACCCC AACGGCTACC CCTTCCTCTT CTGGGAGCAG 
TACATCGGCC TCCGCCACTG GCTGCTGCTG TTCATCAGCG TGGTGTTGGC CTGCACATTC 
CTCGTGTGCG CTGTCTTCCT TCTGAACCCC TGGACGGCCG GGATCATTGT GATGGTCCTG 
GCGCTGATGA CGGTCGAGCT GTTCGGCATG ATGGGCCTCA TCGGAATCAA GCTCAGTGCC 
GTGCCCGTGG TCATCCTGAT CGCTTCTGTT GGCATAGGAG TGGAGTTCAC CGTTCACGTT 
GCTTTGGCCT TTCTGACGGC CATCGGCGAC AAGAACCGCA GGGCTGTGCT TGCCCTGGAG 
CACATGTTTG CACCCGTCCT GGATGGCGCC GTGTCCACTC TGCTGGGAGT GCTGATGCTG 
GCGGGATCTG AGTTCGACTT CATTGTCAGG TATTTCTTTG CTGTGCTGGC GATCCTCACC 
ATCCTCGGCG TTCTCAATGG GCTGGTTTTG CTTCCCGTGC TTTTGTCTTT CTTTGGACCA 
TATCCTGAGG TGTCTCCAGC CAACGGCTTG AACCGCCTGC CCACACCCTC CCCTGAGCCA 
CCCCCCAGCG TGGTCCGCTT CGCCATGCCG CCCGGCCACA CGCACAGCGG GTCTGATTCC 
TCCGACTCGG AGTATAGTTC CCAGACGACA GTGTCAGGCC TCAGCGAGGA GCTTCGGCAC 
TACGAGGCCC AGCAGGGCGC GGGAGGCCCT GCCCACCAAG TGATCGTGGA AGCCACAGAA 
AACCCCGTCT TCGCCCACTC CACTGTGGTC CATCCCGAAT CCAGGCATCA CCCACCCTCG 
AACCCGAGAC AGCAGCCCCA CCTGGACTCA GGGTCCCTGC CTCCCGGACG GCAAGGCCAG 
CAGCCCCGCA GGGACCCCCC CAGAGAAGGC TTGTGGCCAC CCCCCTACAG ACCGCGCAGA 
GACGCTTTTG AAATTTCTAC TGAAGGGCAT TCTGGCCCTA GCAATAGGGC CCGCTGGGGC 
CCTCGCGGGG CCCGTTCTCA CAACCCTCGG AACCCAGCGT CCACTGCCAT GGGCAGCTCC 
GTGCCCGGCT ACTGCCAGCC CATCACCACT GTGACGGCTT CTGCCTCCGT GACTGTCGCC 
GTGCACCCGC CGCCTGTCCC TGGGCCTGGG CGGAACCCCC GAGGGGGACT CTGCCCAGGC 
TACCCTGAGA CTGACCACGG CCTGTTTGAG GACCCCCACG TGCCTTTCCA CGTCCGGTGT 
GAGAGGAGGG ATTCGAAGGT GGAAGTCATT GAGCTGCAGG ACGTGGAATG CGAGGAGAGG 
CCCCGGGGAA GCAGCTCCAA CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PTCH1 ( NM_000264.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_000255.2 Homo sapiens patched 1 (PTCH1), transcript variant 1b, mRNA.

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CloneID	OHu18800
Clone ID Related Accession (Same CDS sequence)	NM_000264.4 , NM_000264.3 , NM_000264.5
Accession Version	NM_000264.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4344bp) Protein sequence SNP
Vector	pcDNA3.1⁺/C-(K)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-04-24
Organism	Homo sapiens(Human)
Product	protein patched homolog 1 isoform L
Comment	REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AI494442.1, U59464.1, DB093644.1, AB209495.1, BX117041.1 and CR744004.1. This sequence is a reference standard in the RefSeqGene project. On Mar 29, 2007 this sequence version replaced NM_000264.2. Transcript Variant: This variant (1b) encodes the longest isoform (L). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## CDS exon combination :: U59464.1 [ECO:0000331] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCCTCGG CTGGTAACGC CGCCGAGCCC CAGGACCGCG GCGGCGGCGG CAGCGGCTGT 
ATCGGTGCCC CGGGACGGCC GGCTGGAGGC GGGAGGCGCA GACGGACGGG GGGGCTGCGC 
CGTGCTGCCG CGCCGGACCG GGACTATCTG CACCGGCCCA GCTACTGCGA CGCCGCCTTC 
GCTCTGGAGC AGATTTCCAA GGGGAAGGCT ACTGGCCGGA AAGCGCCGCT GTGGCTGAGA 
GCGAAGTTTC AGAGACTCTT ATTTAAACTG GGTTGTTACA TTCAAAAAAA CTGCGGCAAG 
TTCTTGGTTG TGGGCCTCCT CATATTTGGG GCCTTCGCGG TGGGATTAAA AGCAGCGAAC 
CTCGAGACCA ACGTGGAGGA GCTGTGGGTG GAAGTTGGAG GACGAGTAAG TCGTGAATTA 
AATTATACTC GCCAGAAGAT TGGAGAAGAG GCTATGTTTA ATCCTCAACT CATGATACAG 
ACCCCTAAAG AAGAAGGTGC TAATGTCCTG ACCACAGAAG CGCTCCTACA ACACCTGGAC 
TCGGCACTCC AGGCCAGCCG TGTCCATGTA TACATGTACA ACAGGCAGTG GAAATTGGAA 
CATTTGTGTT ACAAATCAGG AGAGCTTATC ACAGAAACAG GTTACATGGA TCAGATAATA 
GAATATCTTT ACCCTTGTTT GATTATTACA CCTTTGGACT GCTTCTGGGA AGGGGCGAAA 
TTACAGTCTG GGACAGCATA CCTCCTAGGT AAACCTCCTT TGCGGTGGAC AAACTTCGAC 
CCTTTGGAAT TCCTGGAAGA GTTAAAGAAA ATAAACTATC AAGTGGACAG CTGGGAGGAA 
ATGCTGAATA AGGCTGAGGT TGGTCATGGT TACATGGACC GCCCCTGCCT CAATCCGGCC 
GATCCAGACT GCCCCGCCAC AGCCCCCAAC AAAAATTCAA CCAAACCTCT TGATATGGCC 
CTTGTTTTGA ATGGTGGATG TCATGGCTTA TCCAGAAAGT ATATGCACTG GCAGGAGGAG 
TTGATTGTGG GTGGCACAGT CAAGAACAGC ACTGGAAAAC TCGTCAGCGC CCATGCCCTG 
CAGACCATGT TCCAGTTAAT GACTCCCAAG CAAATGTACG AGCACTTCAA GGGGTACGAG 
TATGTCTCAC ACATCAACTG GAACGAGGAC AAAGCGGCAG CCATCCTGGA GGCCTGGCAG 
AGGACATATG TGGAGGTGGT TCATCAGAGT GTCGCACAGA ACTCCACTCA AAAGGTGCTT 
TCCTTCACCA CCACGACCCT GGACGACATC CTGAAATCCT TCTCTGACGT CAGTGTCATC 
CGCGTGGCCA GCGGCTACTT ACTCATGCTC GCCTATGCCT GTCTAACCAT GCTGCGCTGG 
GACTGCTCCA AGTCCCAGGG TGCCGTGGGG CTGGCTGGCG TCCTGCTGGT TGCACTGTCA 
GTGGCTGCAG GACTGGGCCT GTGCTCATTG ATCGGAATTT CCTTTAACGC TGCAACAACT 
CAGGTTTTGC CATTTCTCGC TCTTGGTGTT GGTGTGGATG ATGTTTTTCT TCTGGCCCAC 
GCCTTCAGTG AAACAGGACA GAATAAAAGA ATCCCTTTTG AGGACAGGAC CGGGGAGTGC 
CTGAAGCGCA CAGGAGCCAG CGTGGCCCTC ACGTCCATCA GCAATGTCAC AGCCTTCTTC 
ATGGCCGCGT TAATCCCAAT TCCCGCTCTG CGGGCGTTCT CCCTCCAGGC AGCGGTAGTA 
GTGGTGTTCA ATTTTGCCAT GGTTCTGCTC ATTTTTCCTG CAATTCTCAG CATGGATTTA 
TATCGACGCG AGGACAGGAG ACTGGATATT TTCTGCTGTT TTACAAGCCC CTGCGTCAGC 
AGAGTGATTC AGGTTGAACC TCAGGCCTAC ACCGACACAC ACGACAATAC CCGCTACAGC 
CCCCCACCTC CCTACAGCAG CCACAGCTTT GCCCATGAAA CGCAGATTAC CATGCAGTCC 
ACTGTCCAGC TCCGCACGGA GTACGACCCC CACACGCACG TGTACTACAC CACCGCTGAG 
CCGCGCTCCG AGATCTCTGT GCAGCCCGTC ACCGTGACAC AGGACACCCT CAGCTGCCAG 
AGCCCAGAGA GCACCAGCTC CACAAGGGAC CTGCTCTCCC AGTTCTCCGA CTCCAGCCTC 
CACTGCCTCG AGCCCCCCTG TACGAAGTGG ACACTCTCAT CTTTTGCTGA GAAGCACTAT 
GCTCCTTTCC TCTTGAAACC AAAAGCCAAG GTAGTGGTGA TCTTCCTTTT TCTGGGCTTG 
CTGGGGGTCA GCCTTTATGG CACCACCCGA GTGAGAGACG GGCTGGACCT TACGGACATT 
GTACCTCGGG AAACCAGAGA ATATGACTTT ATTGCTGCAC AATTCAAATA CTTTTCTTTC 
TACAACATGT ATATAGTCAC CCAGAAAGCA GACTACCCGA ATATCCAGCA CTTACTTTAC 
GACCTACACA GGAGTTTCAG TAACGTGAAG TATGTCATGT TGGAAGAAAA CAAACAGCTT 
CCCAAAATGT GGCTGCACTA CTTCAGAGAC TGGCTTCAGG GACTTCAGGA TGCATTTGAC 
AGTGACTGGG AAACCGGGAA AATCATGCCA AACAATTACA AGAATGGATC AGACGATGGA 
GTCCTTGCCT ACAAACTCCT GGTGCAAACC GGCAGCCGCG ATAAGCCCAT CGACATCAGC 
CAGTTGACTA AACAGCGTCT GGTGGATGCA GATGGCATCA TTAATCCCAG CGCTTTCTAC 
ATCTACCTGA CGGCTTGGGT CAGCAACGAC CCCGTCGCGT ATGCTGCCTC CCAGGCCAAC 
ATCCGGCCAC ACCGACCAGA ATGGGTCCAC GACAAAGCCG ACTACATGCC TGAAACAAGG 
CTGAGAATCC CGGCAGCAGA GCCCATCGAG TATGCCCAGT TCCCTTTCTA CCTCAACGGC 
TTGCGGGACA CCTCAGACTT TGTGGAGGCA ATTGAAAAAG TAAGGACCAT CTGCAGCAAC 
TATACGAGCC TGGGGCTGTC CAGTTACCCC AACGGCTACC CCTTCCTCTT CTGGGAGCAG 
TACATCGGCC TCCGCCACTG GCTGCTGCTG TTCATCAGCG TGGTGTTGGC CTGCACATTC 
CTCGTGTGCG CTGTCTTCCT TCTGAACCCC TGGACGGCCG GGATCATTGT GATGGTCCTG 
GCGCTGATGA CGGTCGAGCT GTTCGGCATG ATGGGCCTCA TCGGAATCAA GCTCAGTGCC 
GTGCCCGTGG TCATCCTGAT CGCTTCTGTT GGCATAGGAG TGGAGTTCAC CGTTCACGTT 
GCTTTGGCCT TTCTGACGGC CATCGGCGAC AAGAACCGCA GGGCTGTGCT TGCCCTGGAG 
CACATGTTTG CACCCGTCCT GGATGGCGCC GTGTCCACTC TGCTGGGAGT GCTGATGCTG 
GCGGGATCTG AGTTCGACTT CATTGTCAGG TATTTCTTTG CTGTGCTGGC GATCCTCACC 
ATCCTCGGCG TTCTCAATGG GCTGGTTTTG CTTCCCGTGC TTTTGTCTTT CTTTGGACCA 
TATCCTGAGG TGTCTCCAGC CAACGGCTTG AACCGCCTGC CCACACCCTC CCCTGAGCCA 
CCCCCCAGCG TGGTCCGCTT CGCCATGCCG CCCGGCCACA CGCACAGCGG GTCTGATTCC 
TCCGACTCGG AGTATAGTTC CCAGACGACA GTGTCAGGCC TCAGCGAGGA GCTTCGGCAC 
TACGAGGCCC AGCAGGGCGC GGGAGGCCCT GCCCACCAAG TGATCGTGGA AGCCACAGAA 
AACCCCGTCT TCGCCCACTC CACTGTGGTC CATCCCGAAT CCAGGCATCA CCCACCCTCG 
AACCCGAGAC AGCAGCCCCA CCTGGACTCA GGGTCCCTGC CTCCCGGACG GCAAGGCCAG 
CAGCCCCGCA GGGACCCCCC CAGAGAAGGC TTGTGGCCAC CCCCCTACAG ACCGCGCAGA 
GACGCTTTTG AAATTTCTAC TGAAGGGCAT TCTGGCCCTA GCAATAGGGC CCGCTGGGGC 
CCTCGCGGGG CCCGTTCTCA CAACCCTCGG AACCCAGCGT CCACTGCCAT GGGCAGCTCC 
GTGCCCGGCT ACTGCCAGCC CATCACCACT GTGACGGCTT CTGCCTCCGT GACTGTCGCC 
GTGCACCCGC CGCCTGTCCC TGGGCCTGGG CGGAACCCCC GAGGGGGACT CTGCCCAGGC 
TACCCTGAGA CTGACCACGG CCTGTTTGAG GACCCCCACG TGCCTTTCCA CGTCCGGTGT 
GAGAGGAGGG ATTCGAAGGT GGAAGTCATT GAGCTGCAGG ACGTGGAATG CGAGGAGAGG 
CCCCGGGGAA GCAGCTCCAA CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_000255.2
CDS	189..4532
Translation	MASAGNAAEPQDRGGGGSGCIGAPGRPAGGGRRRRTGGLRRAAAPDRDYLHRPSYCDAAFALEQISKGKATGRKAPLWLRAKFQRLLFKLGCYIQKNCGKFLVVGLLIFGAFAVGLKAANLETNVEELWVEVGGRVSRELNYTRQKIGEEAMFNPQLMIQTPKEEGANVLTTEALLQHLDSALQASRVHVYMYNRQWKLEHLCYKSGELITETGYMDQIIEYLYPCLIITPLDCFWEGAKLQSGTAYLLGKPPLRWTNFDPLEFLEELKKINYQVDSWEEMLNKAEVGHGYMDRPCLNPADPDCPATAPNKNSTKPLDMALVLNGGCHGLSRKYMHWQEELIVGGTVKNSTGKLVSAHALQTMFQLMTPKQMYEHFKGYEYVSHINWNEDKAAAILEAWQRTYVEVVHQSVAQNSTQKVLSFTTTTLDDILKSFSDVSVIRVASGYLLMLAYACLTMLRWDCSKSQGAVGLAGVLLVALSVAAGLGLCSLIGISFNAATTQVLPFLALGVGVDDVFLLAHAFSETGQNKRIPFEDRTGECLKRTGASVALTSISNVTAFFMAALIPIPALRAFSLQAAVVVVFNFAMVLLIFPAILSMDLYRREDRRLDIFCCFTSPCVSRVIQVEPQAYTDTHDNTRYSPPPPYSSHSFAHETQITMQSTVQLRTEYDPHTHVYYTTAEPRSEISVQPVTVTQDTLSCQSPESTSSTRDLLSQFSDSSLHCLEPPCTKWTLSSFAEKHYAPFLLKPKAKVVVIFLFLGLLGVSLYGTTRVRDGLDLTDIVPRETREYDFIAAQFKYFSFYNMYIVTQKADYPNIQHLLYDLHRSFSNVKYVMLEENKQLPKMWLHYFRDWLQGLQDAFDSDWETGKIMPNNYKNGSDDGVLAYKLLVQTGSRDKPIDISQLTKQRLVDADGIINPSAFYIYLTAWVSNDPVAYAASQANIRPHRPEWVHDKADYMPETRLRIPAAEPIEYAQFPFYLNGLRDTSDFVEAIEKVRTICSNYTSLGLSSYPNGYPFLFWEQYIGLRHWLLLFISVVLACTFLVCAVFLLNPWTAGIIVMVLALMTVELFGMMGLIGIKLSAVPVVILIASVGIGVEFTVHVALAFLTAIGDKNRRAVLALEHMFAPVLDGAVSTLLGVLMLAGSEFDFIVRYFFAVLAILTILGVLNGLVLLPVLLSFFGPYPEVSPANGLNRLPTPSPEPPPSVVRFAMPPGHTHSGSDSSDSEYSSQTTVSGLSEELRHYEAQQGAGGPAHQVIVEATENPVFAHSTVVHPESRHHPPSNPRQQPHLDSGSLPPGRQGQQPRRDPPREGLWPPPYRPRRDAFEISTEGHSGPSNRARWGPRGARSHNPRNPASTAMGSSVPGYCQPITTVTASASVTVAVHPPPVPGPGRNPRGGLCPGYPETDHGLFEDPHVPFHVRCERRDSKVEVIELQDVECEERPRGSSSN

Target ORF information:

RefSeq Version	NM_000264.3
Organism	Homo sapiens(Human)
Definition	Homo sapiens patched 1 (PTCH1), transcript variant 1b, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_000264.3

ORF Insert Sequence:

ATGGCCTCGG CTGGTAACGC CGCCGAGCCC CAGGACCGCG GCGGCGGCGG CAGCGGCTGT 
ATCGGTGCCC CGGGACGGCC GGCTGGAGGC GGGAGGCGCA GACGGACGGG GGGGCTGCGC 
CGTGCTGCCG CGCCGGACCG GGACTATCTG CACCGGCCCA GCTACTGCGA CGCCGCCTTC 
GCTCTGGAGC AGATTTCCAA GGGGAAGGCT ACTGGCCGGA AAGCGCCGCT GTGGCTGAGA 
GCGAAGTTTC AGAGACTCTT ATTTAAACTG GGTTGTTACA TTCAAAAAAA CTGCGGCAAG 
TTCTTGGTTG TGGGCCTCCT CATATTTGGG GCCTTCGCGG TGGGATTAAA AGCAGCGAAC 
CTCGAGACCA ACGTGGAGGA GCTGTGGGTG GAAGTTGGAG GACGAGTAAG TCGTGAATTA 
AATTATACTC GCCAGAAGAT TGGAGAAGAG GCTATGTTTA ATCCTCAACT CATGATACAG 
ACCCCTAAAG AAGAAGGTGC TAATGTCCTG ACCACAGAAG CGCTCCTACA ACACCTGGAC 
TCGGCACTCC AGGCCAGCCG TGTCCATGTA TACATGTACA ACAGGCAGTG GAAATTGGAA 
CATTTGTGTT ACAAATCAGG AGAGCTTATC ACAGAAACAG GTTACATGGA TCAGATAATA 
GAATATCTTT ACCCTTGTTT GATTATTACA CCTTTGGACT GCTTCTGGGA AGGGGCGAAA 
TTACAGTCTG GGACAGCATA CCTCCTAGGT AAACCTCCTT TGCGGTGGAC AAACTTCGAC 
CCTTTGGAAT TCCTGGAAGA GTTAAAGAAA ATAAACTATC AAGTGGACAG CTGGGAGGAA 
ATGCTGAATA AGGCTGAGGT TGGTCATGGT TACATGGACC GCCCCTGCCT CAATCCGGCC 
GATCCAGACT GCCCCGCCAC AGCCCCCAAC AAAAATTCAA CCAAACCTCT TGATATGGCC 
CTTGTTTTGA ATGGTGGATG TCATGGCTTA TCCAGAAAGT ATATGCACTG GCAGGAGGAG 
TTGATTGTGG GTGGCACAGT CAAGAACAGC ACTGGAAAAC TCGTCAGCGC CCATGCCCTG 
CAGACCATGT TCCAGTTAAT GACTCCCAAG CAAATGTACG AGCACTTCAA GGGGTACGAG 
TATGTCTCAC ACATCAACTG GAACGAGGAC AAAGCGGCAG CCATCCTGGA GGCCTGGCAG 
AGGACATATG TGGAGGTGGT TCATCAGAGT GTCGCACAGA ACTCCACTCA AAAGGTGCTT 
TCCTTCACCA CCACGACCCT GGACGACATC CTGAAATCCT TCTCTGACGT CAGTGTCATC 
CGCGTGGCCA GCGGCTACTT ACTCATGCTC GCCTATGCCT GTCTAACCAT GCTGCGCTGG 
GACTGCTCCA AGTCCCAGGG TGCCGTGGGG CTGGCTGGCG TCCTGCTGGT TGCACTGTCA 
GTGGCTGCAG GACTGGGCCT GTGCTCATTG ATCGGAATTT CCTTTAACGC TGCAACAACT 
CAGGTTTTGC CATTTCTCGC TCTTGGTGTT GGTGTGGATG ATGTTTTTCT TCTGGCCCAC 
GCCTTCAGTG AAACAGGACA GAATAAAAGA ATCCCTTTTG AGGACAGGAC CGGGGAGTGC 
CTGAAGCGCA CAGGAGCCAG CGTGGCCCTC ACGTCCATCA GCAATGTCAC AGCCTTCTTC 
ATGGCCGCGT TAATCCCAAT TCCCGCTCTG CGGGCGTTCT CCCTCCAGGC AGCGGTAGTA 
GTGGTGTTCA ATTTTGCCAT GGTTCTGCTC ATTTTTCCTG CAATTCTCAG CATGGATTTA 
TATCGACGCG AGGACAGGAG ACTGGATATT TTCTGCTGTT TTACAAGCCC CTGCGTCAGC 
AGAGTGATTC AGGTTGAACC TCAGGCCTAC ACCGACACAC ACGACAATAC CCGCTACAGC 
CCCCCACCTC CCTACAGCAG CCACAGCTTT GCCCATGAAA CGCAGATTAC CATGCAGTCC 
ACTGTCCAGC TCCGCACGGA GTACGACCCC CACACGCACG TGTACTACAC CACCGCTGAG 
CCGCGCTCCG AGATCTCTGT GCAGCCCGTC ACCGTGACAC AGGACACCCT CAGCTGCCAG 
AGCCCAGAGA GCACCAGCTC CACAAGGGAC CTGCTCTCCC AGTTCTCCGA CTCCAGCCTC 
CACTGCCTCG AGCCCCCCTG TACGAAGTGG ACACTCTCAT CTTTTGCTGA GAAGCACTAT 
GCTCCTTTCC TCTTGAAACC AAAAGCCAAG GTAGTGGTGA TCTTCCTTTT TCTGGGCTTG 
CTGGGGGTCA GCCTTTATGG CACCACCCGA GTGAGAGACG GGCTGGACCT TACGGACATT 
GTACCTCGGG AAACCAGAGA ATATGACTTT ATTGCTGCAC AATTCAAATA CTTTTCTTTC 
TACAACATGT ATATAGTCAC CCAGAAAGCA GACTACCCGA ATATCCAGCA CTTACTTTAC 
GACCTACACA GGAGTTTCAG TAACGTGAAG TATGTCATGT TGGAAGAAAA CAAACAGCTT 
CCCAAAATGT GGCTGCACTA CTTCAGAGAC TGGCTTCAGG GACTTCAGGA TGCATTTGAC 
AGTGACTGGG AAACCGGGAA AATCATGCCA AACAATTACA AGAATGGATC AGACGATGGA 
GTCCTTGCCT ACAAACTCCT GGTGCAAACC GGCAGCCGCG ATAAGCCCAT CGACATCAGC 
CAGTTGACTA AACAGCGTCT GGTGGATGCA GATGGCATCA TTAATCCCAG CGCTTTCTAC 
ATCTACCTGA CGGCTTGGGT CAGCAACGAC CCCGTCGCGT ATGCTGCCTC CCAGGCCAAC 
ATCCGGCCAC ACCGACCAGA ATGGGTCCAC GACAAAGCCG ACTACATGCC TGAAACAAGG 
CTGAGAATCC CGGCAGCAGA GCCCATCGAG TATGCCCAGT TCCCTTTCTA CCTCAACGGC 
TTGCGGGACA CCTCAGACTT TGTGGAGGCA ATTGAAAAAG TAAGGACCAT CTGCAGCAAC 
TATACGAGCC TGGGGCTGTC CAGTTACCCC AACGGCTACC CCTTCCTCTT CTGGGAGCAG 
TACATCGGCC TCCGCCACTG GCTGCTGCTG TTCATCAGCG TGGTGTTGGC CTGCACATTC 
CTCGTGTGCG CTGTCTTCCT TCTGAACCCC TGGACGGCCG GGATCATTGT GATGGTCCTG 
GCGCTGATGA CGGTCGAGCT GTTCGGCATG ATGGGCCTCA TCGGAATCAA GCTCAGTGCC 
GTGCCCGTGG TCATCCTGAT CGCTTCTGTT GGCATAGGAG TGGAGTTCAC CGTTCACGTT 
GCTTTGGCCT TTCTGACGGC CATCGGCGAC AAGAACCGCA GGGCTGTGCT TGCCCTGGAG 
CACATGTTTG CACCCGTCCT GGATGGCGCC GTGTCCACTC TGCTGGGAGT GCTGATGCTG 
GCGGGATCTG AGTTCGACTT CATTGTCAGG TATTTCTTTG CTGTGCTGGC GATCCTCACC 
ATCCTCGGCG TTCTCAATGG GCTGGTTTTG CTTCCCGTGC TTTTGTCTTT CTTTGGACCA 
TATCCTGAGG TGTCTCCAGC CAACGGCTTG AACCGCCTGC CCACACCCTC CCCTGAGCCA 
CCCCCCAGCG TGGTCCGCTT CGCCATGCCG CCCGGCCACA CGCACAGCGG GTCTGATTCC 
TCCGACTCGG AGTATAGTTC CCAGACGACA GTGTCAGGCC TCAGCGAGGA GCTTCGGCAC 
TACGAGGCCC AGCAGGGCGC GGGAGGCCCT GCCCACCAAG TGATCGTGGA AGCCACAGAA 
AACCCCGTCT TCGCCCACTC CACTGTGGTC CATCCCGAAT CCAGGCATCA CCCACCCTCG 
AACCCGAGAC AGCAGCCCCA CCTGGACTCA GGGTCCCTGC CTCCCGGACG GCAAGGCCAG 
CAGCCCCGCA GGGACCCCCC CAGAGAAGGC TTGTGGCCAC CCCCCTACAG ACCGCGCAGA 
GACGCTTTTG AAATTTCTAC TGAAGGGCAT TCTGGCCCTA GCAATAGGGC CCGCTGGGGC 
CCTCGCGGGG CCCGTTCTCA CAACCCTCGG AACCCAGCGT CCACTGCCAT GGGCAGCTCC 
GTGCCCGGCT ACTGCCAGCC CATCACCACT GTGACGGCTT CTGCCTCCGT GACTGTCGCC 
GTGCACCCGC CGCCTGTCCC TGGGCCTGGG CGGAACCCCC GAGGGGGACT CTGCCCAGGC 
TACCCTGAGA CTGACCACGG CCTGTTTGAG GACCCCCACG TGCCTTTCCA CGTCCGGTGT 
GAGAGGAGGG ATTCGAAGGT GGAAGTCATT GAGCTGCAGG ACGTGGAATG CGAGGAGAGG 
CCCCGGGGAA GCAGCTCCAA CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PTCH1 ( NM_000264.5 ) cDNA ORF clone, Homo sapiens(human) -> NP_000255.2 Homo sapiens patched 1 (PTCH1), transcript variant 1b, mRNA.

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CloneID	OHu18800
Clone ID Related Accession (Same CDS sequence)	NM_000264.4 , NM_000264.3 , NM_000264.5
Accession Version	NM_000264.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4344bp) Protein sequence SNP
Vector	pcDNA3.1⁺/C-(K)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-07-25
Organism	Homo sapiens(human)
Product	protein patched homolog 1 isoform L
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL161729.27. On Jul 26, 2019 this sequence version replaced NM_000264.4. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## CDS exon combination :: U59464.1 [ECO:0000331] ##Evidence-Data-END## ##RefSeq-Attributes-START## RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGGCCTCGG CTGGTAACGC CGCCGAGCCC CAGGACCGCG GCGGCGGCGG CAGCGGCTGT 
ATCGGTGCCC CGGGACGGCC GGCTGGAGGC GGGAGGCGCA GACGGACGGG GGGGCTGCGC 
CGTGCTGCCG CGCCGGACCG GGACTATCTG CACCGGCCCA GCTACTGCGA CGCCGCCTTC 
GCTCTGGAGC AGATTTCCAA GGGGAAGGCT ACTGGCCGGA AAGCGCCGCT GTGGCTGAGA 
GCGAAGTTTC AGAGACTCTT ATTTAAACTG GGTTGTTACA TTCAAAAAAA CTGCGGCAAG 
TTCTTGGTTG TGGGCCTCCT CATATTTGGG GCCTTCGCGG TGGGATTAAA AGCAGCGAAC 
CTCGAGACCA ACGTGGAGGA GCTGTGGGTG GAAGTTGGAG GACGAGTAAG TCGTGAATTA 
AATTATACTC GCCAGAAGAT TGGAGAAGAG GCTATGTTTA ATCCTCAACT CATGATACAG 
ACCCCTAAAG AAGAAGGTGC TAATGTCCTG ACCACAGAAG CGCTCCTACA ACACCTGGAC 
TCGGCACTCC AGGCCAGCCG TGTCCATGTA TACATGTACA ACAGGCAGTG GAAATTGGAA 
CATTTGTGTT ACAAATCAGG AGAGCTTATC ACAGAAACAG GTTACATGGA TCAGATAATA 
GAATATCTTT ACCCTTGTTT GATTATTACA CCTTTGGACT GCTTCTGGGA AGGGGCGAAA 
TTACAGTCTG GGACAGCATA CCTCCTAGGT AAACCTCCTT TGCGGTGGAC AAACTTCGAC 
CCTTTGGAAT TCCTGGAAGA GTTAAAGAAA ATAAACTATC AAGTGGACAG CTGGGAGGAA 
ATGCTGAATA AGGCTGAGGT TGGTCATGGT TACATGGACC GCCCCTGCCT CAATCCGGCC 
GATCCAGACT GCCCCGCCAC AGCCCCCAAC AAAAATTCAA CCAAACCTCT TGATATGGCC 
CTTGTTTTGA ATGGTGGATG TCATGGCTTA TCCAGAAAGT ATATGCACTG GCAGGAGGAG 
TTGATTGTGG GTGGCACAGT CAAGAACAGC ACTGGAAAAC TCGTCAGCGC CCATGCCCTG 
CAGACCATGT TCCAGTTAAT GACTCCCAAG CAAATGTACG AGCACTTCAA GGGGTACGAG 
TATGTCTCAC ACATCAACTG GAACGAGGAC AAAGCGGCAG CCATCCTGGA GGCCTGGCAG 
AGGACATATG TGGAGGTGGT TCATCAGAGT GTCGCACAGA ACTCCACTCA AAAGGTGCTT 
TCCTTCACCA CCACGACCCT GGACGACATC CTGAAATCCT TCTCTGACGT CAGTGTCATC 
CGCGTGGCCA GCGGCTACTT ACTCATGCTC GCCTATGCCT GTCTAACCAT GCTGCGCTGG 
GACTGCTCCA AGTCCCAGGG TGCCGTGGGG CTGGCTGGCG TCCTGCTGGT TGCACTGTCA 
GTGGCTGCAG GACTGGGCCT GTGCTCATTG ATCGGAATTT CCTTTAACGC TGCAACAACT 
CAGGTTTTGC CATTTCTCGC TCTTGGTGTT GGTGTGGATG ATGTTTTTCT TCTGGCCCAC 
GCCTTCAGTG AAACAGGACA GAATAAAAGA ATCCCTTTTG AGGACAGGAC CGGGGAGTGC 
CTGAAGCGCA CAGGAGCCAG CGTGGCCCTC ACGTCCATCA GCAATGTCAC AGCCTTCTTC 
ATGGCCGCGT TAATCCCAAT TCCCGCTCTG CGGGCGTTCT CCCTCCAGGC AGCGGTAGTA 
GTGGTGTTCA ATTTTGCCAT GGTTCTGCTC ATTTTTCCTG CAATTCTCAG CATGGATTTA 
TATCGACGCG AGGACAGGAG ACTGGATATT TTCTGCTGTT TTACAAGCCC CTGCGTCAGC 
AGAGTGATTC AGGTTGAACC TCAGGCCTAC ACCGACACAC ACGACAATAC CCGCTACAGC 
CCCCCACCTC CCTACAGCAG CCACAGCTTT GCCCATGAAA CGCAGATTAC CATGCAGTCC 
ACTGTCCAGC TCCGCACGGA GTACGACCCC CACACGCACG TGTACTACAC CACCGCTGAG 
CCGCGCTCCG AGATCTCTGT GCAGCCCGTC ACCGTGACAC AGGACACCCT CAGCTGCCAG 
AGCCCAGAGA GCACCAGCTC CACAAGGGAC CTGCTCTCCC AGTTCTCCGA CTCCAGCCTC 
CACTGCCTCG AGCCCCCCTG TACGAAGTGG ACACTCTCAT CTTTTGCTGA GAAGCACTAT 
GCTCCTTTCC TCTTGAAACC AAAAGCCAAG GTAGTGGTGA TCTTCCTTTT TCTGGGCTTG 
CTGGGGGTCA GCCTTTATGG CACCACCCGA GTGAGAGACG GGCTGGACCT TACGGACATT 
GTACCTCGGG AAACCAGAGA ATATGACTTT ATTGCTGCAC AATTCAAATA CTTTTCTTTC 
TACAACATGT ATATAGTCAC CCAGAAAGCA GACTACCCGA ATATCCAGCA CTTACTTTAC 
GACCTACACA GGAGTTTCAG TAACGTGAAG TATGTCATGT TGGAAGAAAA CAAACAGCTT 
CCCAAAATGT GGCTGCACTA CTTCAGAGAC TGGCTTCAGG GACTTCAGGA TGCATTTGAC 
AGTGACTGGG AAACCGGGAA AATCATGCCA AACAATTACA AGAATGGATC AGACGATGGA 
GTCCTTGCCT ACAAACTCCT GGTGCAAACC GGCAGCCGCG ATAAGCCCAT CGACATCAGC 
CAGTTGACTA AACAGCGTCT GGTGGATGCA GATGGCATCA TTAATCCCAG CGCTTTCTAC 
ATCTACCTGA CGGCTTGGGT CAGCAACGAC CCCGTCGCGT ATGCTGCCTC CCAGGCCAAC 
ATCCGGCCAC ACCGACCAGA ATGGGTCCAC GACAAAGCCG ACTACATGCC TGAAACAAGG 
CTGAGAATCC CGGCAGCAGA GCCCATCGAG TATGCCCAGT TCCCTTTCTA CCTCAACGGC 
TTGCGGGACA CCTCAGACTT TGTGGAGGCA ATTGAAAAAG TAAGGACCAT CTGCAGCAAC 
TATACGAGCC TGGGGCTGTC CAGTTACCCC AACGGCTACC CCTTCCTCTT CTGGGAGCAG 
TACATCGGCC TCCGCCACTG GCTGCTGCTG TTCATCAGCG TGGTGTTGGC CTGCACATTC 
CTCGTGTGCG CTGTCTTCCT TCTGAACCCC TGGACGGCCG GGATCATTGT GATGGTCCTG 
GCGCTGATGA CGGTCGAGCT GTTCGGCATG ATGGGCCTCA TCGGAATCAA GCTCAGTGCC 
GTGCCCGTGG TCATCCTGAT CGCTTCTGTT GGCATAGGAG TGGAGTTCAC CGTTCACGTT 
GCTTTGGCCT TTCTGACGGC CATCGGCGAC AAGAACCGCA GGGCTGTGCT TGCCCTGGAG 
CACATGTTTG CACCCGTCCT GGATGGCGCC GTGTCCACTC TGCTGGGAGT GCTGATGCTG 
GCGGGATCTG AGTTCGACTT CATTGTCAGG TATTTCTTTG CTGTGCTGGC GATCCTCACC 
ATCCTCGGCG TTCTCAATGG GCTGGTTTTG CTTCCCGTGC TTTTGTCTTT CTTTGGACCA 
TATCCTGAGG TGTCTCCAGC CAACGGCTTG AACCGCCTGC CCACACCCTC CCCTGAGCCA 
CCCCCCAGCG TGGTCCGCTT CGCCATGCCG CCCGGCCACA CGCACAGCGG GTCTGATTCC 
TCCGACTCGG AGTATAGTTC CCAGACGACA GTGTCAGGCC TCAGCGAGGA GCTTCGGCAC 
TACGAGGCCC AGCAGGGCGC GGGAGGCCCT GCCCACCAAG TGATCGTGGA AGCCACAGAA 
AACCCCGTCT TCGCCCACTC CACTGTGGTC CATCCCGAAT CCAGGCATCA CCCACCCTCG 
AACCCGAGAC AGCAGCCCCA CCTGGACTCA GGGTCCCTGC CTCCCGGACG GCAAGGCCAG 
CAGCCCCGCA GGGACCCCCC CAGAGAAGGC TTGTGGCCAC CCCCCTACAG ACCGCGCAGA 
GACGCTTTTG AAATTTCTAC TGAAGGGCAT TCTGGCCCTA GCAATAGGGC CCGCTGGGGC 
CCTCGCGGGG CCCGTTCTCA CAACCCTCGG AACCCAGCGT CCACTGCCAT GGGCAGCTCC 
GTGCCCGGCT ACTGCCAGCC CATCACCACT GTGACGGCTT CTGCCTCCGT GACTGTCGCC 
GTGCACCCGC CGCCTGTCCC TGGGCCTGGG CGGAACCCCC GAGGGGGACT CTGCCCAGGC 
TACCCTGAGA CTGACCACGG CCTGTTTGAG GACCCCCACG TGCCTTTCCA CGTCCGGTGT 
GAGAGGAGGG ATTCGAAGGT GGAAGTCATT GAGCTGCAGG ACGTGGAATG CGAGGAGAGG 
CCCCGGGGAA GCAGCTCCAA CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_000255.2
CDS	906..5249
Translation	MASAGNAAEPQDRGGGGSGCIGAPGRPAGGGRRRRTGGLRRAAAPDRDYLHRPSYCDAAFALEQISKGKATGRKAPLWLRAKFQRLLFKLGCYIQKNCGKFLVVGLLIFGAFAVGLKAANLETNVEELWVEVGGRVSRELNYTRQKIGEEAMFNPQLMIQTPKEEGANVLTTEALLQHLDSALQASRVHVYMYNRQWKLEHLCYKSGELITETGYMDQIIEYLYPCLIITPLDCFWEGAKLQSGTAYLLGKPPLRWTNFDPLEFLEELKKINYQVDSWEEMLNKAEVGHGYMDRPCLNPADPDCPATAPNKNSTKPLDMALVLNGGCHGLSRKYMHWQEELIVGGTVKNSTGKLVSAHALQTMFQLMTPKQMYEHFKGYEYVSHINWNEDKAAAILEAWQRTYVEVVHQSVAQNSTQKVLSFTTTTLDDILKSFSDVSVIRVASGYLLMLAYACLTMLRWDCSKSQGAVGLAGVLLVALSVAAGLGLCSLIGISFNAATTQVLPFLALGVGVDDVFLLAHAFSETGQNKRIPFEDRTGECLKRTGASVALTSISNVTAFFMAALIPIPALRAFSLQAAVVVVFNFAMVLLIFPAILSMDLYRREDRRLDIFCCFTSPCVSRVIQVEPQAYTDTHDNTRYSPPPPYSSHSFAHETQITMQSTVQLRTEYDPHTHVYYTTAEPRSEISVQPVTVTQDTLSCQSPESTSSTRDLLSQFSDSSLHCLEPPCTKWTLSSFAEKHYAPFLLKPKAKVVVIFLFLGLLGVSLYGTTRVRDGLDLTDIVPRETREYDFIAAQFKYFSFYNMYIVTQKADYPNIQHLLYDLHRSFSNVKYVMLEENKQLPKMWLHYFRDWLQGLQDAFDSDWETGKIMPNNYKNGSDDGVLAYKLLVQTGSRDKPIDISQLTKQRLVDADGIINPSAFYIYLTAWVSNDPVAYAASQANIRPHRPEWVHDKADYMPETRLRIPAAEPIEYAQFPFYLNGLRDTSDFVEAIEKVRTICSNYTSLGLSSYPNGYPFLFWEQYIGLRHWLLLFISVVLACTFLVCAVFLLNPWTAGIIVMVLALMTVELFGMMGLIGIKLSAVPVVILIASVGIGVEFTVHVALAFLTAIGDKNRRAVLALEHMFAPVLDGAVSTLLGVLMLAGSEFDFIVRYFFAVLAILTILGVLNGLVLLPVLLSFFGPYPEVSPANGLNRLPTPSPEPPPSVVRFAMPPGHTHSGSDSSDSEYSSQTTVSGLSEELRHYEAQQGAGGPAHQVIVEATENPVFAHSTVVHPESRHHPPSNPRQQPHLDSGSLPPGRQGQQPRRDPPREGLWPPPYRPRRDAFEISTEGHSGPSNRARWGPRGARSHNPRNPASTAMGSSVPGYCQPITTVTASASVTVAVHPPPVPGPGRNPRGGLCPGYPETDHGLFEDPHVPFHVRCERRDSKVEVIELQDVECEERPRGSSSN

Target ORF information:

RefSeq Version	NM_000264.5
Organism	Homo sapiens(human)
Definition	Homo sapiens patched 1 (PTCH1), transcript variant 1b, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_000264.5

ORF Insert Sequence:

ATGGCCTCGG CTGGTAACGC CGCCGAGCCC CAGGACCGCG GCGGCGGCGG CAGCGGCTGT 
ATCGGTGCCC CGGGACGGCC GGCTGGAGGC GGGAGGCGCA GACGGACGGG GGGGCTGCGC 
CGTGCTGCCG CGCCGGACCG GGACTATCTG CACCGGCCCA GCTACTGCGA CGCCGCCTTC 
GCTCTGGAGC AGATTTCCAA GGGGAAGGCT ACTGGCCGGA AAGCGCCGCT GTGGCTGAGA 
GCGAAGTTTC AGAGACTCTT ATTTAAACTG GGTTGTTACA TTCAAAAAAA CTGCGGCAAG 
TTCTTGGTTG TGGGCCTCCT CATATTTGGG GCCTTCGCGG TGGGATTAAA AGCAGCGAAC 
CTCGAGACCA ACGTGGAGGA GCTGTGGGTG GAAGTTGGAG GACGAGTAAG TCGTGAATTA 
AATTATACTC GCCAGAAGAT TGGAGAAGAG GCTATGTTTA ATCCTCAACT CATGATACAG 
ACCCCTAAAG AAGAAGGTGC TAATGTCCTG ACCACAGAAG CGCTCCTACA ACACCTGGAC 
TCGGCACTCC AGGCCAGCCG TGTCCATGTA TACATGTACA ACAGGCAGTG GAAATTGGAA 
CATTTGTGTT ACAAATCAGG AGAGCTTATC ACAGAAACAG GTTACATGGA TCAGATAATA 
GAATATCTTT ACCCTTGTTT GATTATTACA CCTTTGGACT GCTTCTGGGA AGGGGCGAAA 
TTACAGTCTG GGACAGCATA CCTCCTAGGT AAACCTCCTT TGCGGTGGAC AAACTTCGAC 
CCTTTGGAAT TCCTGGAAGA GTTAAAGAAA ATAAACTATC AAGTGGACAG CTGGGAGGAA 
ATGCTGAATA AGGCTGAGGT TGGTCATGGT TACATGGACC GCCCCTGCCT CAATCCGGCC 
GATCCAGACT GCCCCGCCAC AGCCCCCAAC AAAAATTCAA CCAAACCTCT TGATATGGCC 
CTTGTTTTGA ATGGTGGATG TCATGGCTTA TCCAGAAAGT ATATGCACTG GCAGGAGGAG 
TTGATTGTGG GTGGCACAGT CAAGAACAGC ACTGGAAAAC TCGTCAGCGC CCATGCCCTG 
CAGACCATGT TCCAGTTAAT GACTCCCAAG CAAATGTACG AGCACTTCAA GGGGTACGAG 
TATGTCTCAC ACATCAACTG GAACGAGGAC AAAGCGGCAG CCATCCTGGA GGCCTGGCAG 
AGGACATATG TGGAGGTGGT TCATCAGAGT GTCGCACAGA ACTCCACTCA AAAGGTGCTT 
TCCTTCACCA CCACGACCCT GGACGACATC CTGAAATCCT TCTCTGACGT CAGTGTCATC 
CGCGTGGCCA GCGGCTACTT ACTCATGCTC GCCTATGCCT GTCTAACCAT GCTGCGCTGG 
GACTGCTCCA AGTCCCAGGG TGCCGTGGGG CTGGCTGGCG TCCTGCTGGT TGCACTGTCA 
GTGGCTGCAG GACTGGGCCT GTGCTCATTG ATCGGAATTT CCTTTAACGC TGCAACAACT 
CAGGTTTTGC CATTTCTCGC TCTTGGTGTT GGTGTGGATG ATGTTTTTCT TCTGGCCCAC 
GCCTTCAGTG AAACAGGACA GAATAAAAGA ATCCCTTTTG AGGACAGGAC CGGGGAGTGC 
CTGAAGCGCA CAGGAGCCAG CGTGGCCCTC ACGTCCATCA GCAATGTCAC AGCCTTCTTC 
ATGGCCGCGT TAATCCCAAT TCCCGCTCTG CGGGCGTTCT CCCTCCAGGC AGCGGTAGTA 
GTGGTGTTCA ATTTTGCCAT GGTTCTGCTC ATTTTTCCTG CAATTCTCAG CATGGATTTA 
TATCGACGCG AGGACAGGAG ACTGGATATT TTCTGCTGTT TTACAAGCCC CTGCGTCAGC 
AGAGTGATTC AGGTTGAACC TCAGGCCTAC ACCGACACAC ACGACAATAC CCGCTACAGC 
CCCCCACCTC CCTACAGCAG CCACAGCTTT GCCCATGAAA CGCAGATTAC CATGCAGTCC 
ACTGTCCAGC TCCGCACGGA GTACGACCCC CACACGCACG TGTACTACAC CACCGCTGAG 
CCGCGCTCCG AGATCTCTGT GCAGCCCGTC ACCGTGACAC AGGACACCCT CAGCTGCCAG 
AGCCCAGAGA GCACCAGCTC CACAAGGGAC CTGCTCTCCC AGTTCTCCGA CTCCAGCCTC 
CACTGCCTCG AGCCCCCCTG TACGAAGTGG ACACTCTCAT CTTTTGCTGA GAAGCACTAT 
GCTCCTTTCC TCTTGAAACC AAAAGCCAAG GTAGTGGTGA TCTTCCTTTT TCTGGGCTTG 
CTGGGGGTCA GCCTTTATGG CACCACCCGA GTGAGAGACG GGCTGGACCT TACGGACATT 
GTACCTCGGG AAACCAGAGA ATATGACTTT ATTGCTGCAC AATTCAAATA CTTTTCTTTC 
TACAACATGT ATATAGTCAC CCAGAAAGCA GACTACCCGA ATATCCAGCA CTTACTTTAC 
GACCTACACA GGAGTTTCAG TAACGTGAAG TATGTCATGT TGGAAGAAAA CAAACAGCTT 
CCCAAAATGT GGCTGCACTA CTTCAGAGAC TGGCTTCAGG GACTTCAGGA TGCATTTGAC 
AGTGACTGGG AAACCGGGAA AATCATGCCA AACAATTACA AGAATGGATC AGACGATGGA 
GTCCTTGCCT ACAAACTCCT GGTGCAAACC GGCAGCCGCG ATAAGCCCAT CGACATCAGC 
CAGTTGACTA AACAGCGTCT GGTGGATGCA GATGGCATCA TTAATCCCAG CGCTTTCTAC 
ATCTACCTGA CGGCTTGGGT CAGCAACGAC CCCGTCGCGT ATGCTGCCTC CCAGGCCAAC 
ATCCGGCCAC ACCGACCAGA ATGGGTCCAC GACAAAGCCG ACTACATGCC TGAAACAAGG 
CTGAGAATCC CGGCAGCAGA GCCCATCGAG TATGCCCAGT TCCCTTTCTA CCTCAACGGC 
TTGCGGGACA CCTCAGACTT TGTGGAGGCA ATTGAAAAAG TAAGGACCAT CTGCAGCAAC 
TATACGAGCC TGGGGCTGTC CAGTTACCCC AACGGCTACC CCTTCCTCTT CTGGGAGCAG 
TACATCGGCC TCCGCCACTG GCTGCTGCTG TTCATCAGCG TGGTGTTGGC CTGCACATTC 
CTCGTGTGCG CTGTCTTCCT TCTGAACCCC TGGACGGCCG GGATCATTGT GATGGTCCTG 
GCGCTGATGA CGGTCGAGCT GTTCGGCATG ATGGGCCTCA TCGGAATCAA GCTCAGTGCC 
GTGCCCGTGG TCATCCTGAT CGCTTCTGTT GGCATAGGAG TGGAGTTCAC CGTTCACGTT 
GCTTTGGCCT TTCTGACGGC CATCGGCGAC AAGAACCGCA GGGCTGTGCT TGCCCTGGAG 
CACATGTTTG CACCCGTCCT GGATGGCGCC GTGTCCACTC TGCTGGGAGT GCTGATGCTG 
GCGGGATCTG AGTTCGACTT CATTGTCAGG TATTTCTTTG CTGTGCTGGC GATCCTCACC 
ATCCTCGGCG TTCTCAATGG GCTGGTTTTG CTTCCCGTGC TTTTGTCTTT CTTTGGACCA 
TATCCTGAGG TGTCTCCAGC CAACGGCTTG AACCGCCTGC CCACACCCTC CCCTGAGCCA 
CCCCCCAGCG TGGTCCGCTT CGCCATGCCG CCCGGCCACA CGCACAGCGG GTCTGATTCC 
TCCGACTCGG AGTATAGTTC CCAGACGACA GTGTCAGGCC TCAGCGAGGA GCTTCGGCAC 
TACGAGGCCC AGCAGGGCGC GGGAGGCCCT GCCCACCAAG TGATCGTGGA AGCCACAGAA 
AACCCCGTCT TCGCCCACTC CACTGTGGTC CATCCCGAAT CCAGGCATCA CCCACCCTCG 
AACCCGAGAC AGCAGCCCCA CCTGGACTCA GGGTCCCTGC CTCCCGGACG GCAAGGCCAG 
CAGCCCCGCA GGGACCCCCC CAGAGAAGGC TTGTGGCCAC CCCCCTACAG ACCGCGCAGA 
GACGCTTTTG AAATTTCTAC TGAAGGGCAT TCTGGCCCTA GCAATAGGGC CCGCTGGGGC 
CCTCGCGGGG CCCGTTCTCA CAACCCTCGG AACCCAGCGT CCACTGCCAT GGGCAGCTCC 
GTGCCCGGCT ACTGCCAGCC CATCACCACT GTGACGGCTT CTGCCTCCGT GACTGTCGCC 
GTGCACCCGC CGCCTGTCCC TGGGCCTGGG CGGAACCCCC GAGGGGGACT CTGCCCAGGC 
TACCCTGAGA CTGACCACGG CCTGTTTGAG GACCCCCACG TGCCTTTCCA CGTCCGGTGT 
GAGAGGAGGG ATTCGAAGGT GGAAGTCATT GAGCTGCAGG ACGTGGAATG CGAGGAGAGG 
CCCCGGGGAA GCAGCTCCAA CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PTCH1 ( XM_005252102.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_005252159.1 Homo sapiens patched 1 (PTCH1), transcript variant X5, mRNA.

Price & Availability↑

CloneID	OHu17265
Clone ID Related Accession (Same CDS sequence)	XM_005252102.2 , XM_011518872.1 , NM_001083605.1 , NM_001083604.2 , NM_001083606.2 , NM_001083606.1 , XM_011518871.1 , XM_011518869.1 , NM_001083604.1 , XM_011518870.1 , NM_001083607.1 , NM_001083606.3 , NM_001083605.2 , NM_001083607.2
Accession Version	XM_005252102.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3891bp) Protein sequence SNP
Vector	pcDNA3.1⁺/C-(K)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-03-11
Organism	Homo sapiens(Human)
Product	protein patched homolog 1 isoform X1
Comment

ATGTTTAATC CTCAACTCAT GATACAGACC CCTAAAGAAG AAGGTGCTAA TGTCCTGACC 
ACAGAAGCGC TCCTACAACA CCTGGACTCG GCACTCCAGG CCAGCCGTGT CCATGTATAC 
ATGTACAACA GGCAGTGGAA ATTGGAACAT TTGTGTTACA AATCAGGAGA GCTTATCACA 
GAAACAGGTT ACATGGATCA GATAATAGAA TATCTTTACC CTTGTTTGAT TATTACACCT 
TTGGACTGCT TCTGGGAAGG GGCGAAATTA CAGTCTGGGA CAGCATACCT CCTAGGTAAA 
CCTCCTTTGC GGTGGACAAA CTTCGACCCT TTGGAATTCC TGGAAGAGTT AAAGAAAATA 
AACTATCAAG TGGACAGCTG GGAGGAAATG CTGAATAAGG CTGAGGTTGG TCATGGTTAC 
ATGGACCGCC CCTGCCTCAA TCCGGCCGAT CCAGACTGCC CCGCCACAGC CCCCAACAAA 
AATTCAACCA AACCTCTTGA TATGGCCCTT GTTTTGAATG GTGGATGTCA TGGCTTATCC 
AGAAAGTATA TGCACTGGCA GGAGGAGTTG ATTGTGGGTG GCACAGTCAA GAACAGCACT 
GGAAAACTCG TCAGCGCCCA TGCCCTGCAG ACCATGTTCC AGTTAATGAC TCCCAAGCAA 
ATGTACGAGC ACTTCAAGGG GTACGAGTAT GTCTCACACA TCAACTGGAA CGAGGACAAA 
GCGGCAGCCA TCCTGGAGGC CTGGCAGAGG ACATATGTGG AGGTGGTTCA TCAGAGTGTC 
GCACAGAACT CCACTCAAAA GGTGCTTTCC TTCACCACCA CGACCCTGGA CGACATCCTG 
AAATCCTTCT CTGACGTCAG TGTCATCCGC GTGGCCAGCG GCTACTTACT CATGCTCGCC 
TATGCCTGTC TAACCATGCT GCGCTGGGAC TGCTCCAAGT CCCAGGGTGC CGTGGGGCTG 
GCTGGCGTCC TGCTGGTTGC ACTGTCAGTG GCTGCAGGAC TGGGCCTGTG CTCATTGATC 
GGAATTTCCT TTAACGCTGC AACAACTCAG GTTTTGCCAT TTCTCGCTCT TGGTGTTGGT 
GTGGATGATG TTTTTCTTCT GGCCCACGCC TTCAGTGAAA CAGGACAGAA TAAAAGAATC 
CCTTTTGAGG ACAGGACCGG GGAGTGCCTG AAGCGCACAG GAGCCAGCGT GGCCCTCACG 
TCCATCAGCA ATGTCACAGC CTTCTTCATG GCCGCGTTAA TCCCAATTCC CGCTCTGCGG 
GCGTTCTCCC TCCAGGCAGC GGTAGTAGTG GTGTTCAATT TTGCCATGGT TCTGCTCATT 
TTTCCTGCAA TTCTCAGCAT GGATTTATAT CGACGCGAGG ACAGGAGACT GGATATTTTC 
TGCTGTTTTA CAAGCCCCTG CGTCAGCAGA GTGATTCAGG TTGAACCTCA GGCCTACACC 
GACACACACG ACAATACCCG CTACAGCCCC CCACCTCCCT ACAGCAGCCA CAGCTTTGCC 
CATGAAACGC AGATTACCAT GCAGTCCACT GTCCAGCTCC GCACGGAGTA CGACCCCCAC 
ACGCACGTGT ACTACACCAC CGCTGAGCCG CGCTCCGAGA TCTCTGTGCA GCCCGTCACC 
GTGACACAGG ACACCCTCAG CTGCCAGAGC CCAGAGAGCA CCAGCTCCAC AAGGGACCTG 
CTCTCCCAGT TCTCCGACTC CAGCCTCCAC TGCCTCGAGC CCCCCTGTAC GAAGTGGACA 
CTCTCATCTT TTGCTGAGAA GCACTATGCT CCTTTCCTCT TGAAACCAAA AGCCAAGGTA 
GTGGTGATCT TCCTTTTTCT GGGCTTGCTG GGGGTCAGCC TTTATGGCAC CACCCGAGTG 
AGAGACGGGC TGGACCTTAC GGACATTGTA CCTCGGGAAA CCAGAGAATA TGACTTTATT 
GCTGCACAAT TCAAATACTT TTCTTTCTAC AACATGTATA TAGTCACCCA GAAAGCAGAC 
TACCCGAATA TCCAGCACTT ACTTTACGAC CTACACAGGA GTTTCAGTAA CGTGAAGTAT 
GTCATGTTGG AAGAAAACAA ACAGCTTCCC AAAATGTGGC TGCACTACTT CAGAGACTGG 
CTTCAGGGAC TTCAGGATGC ATTTGACAGT GACTGGGAAA CCGGGAAAAT CATGCCAAAC 
AATTACAAGA ATGGATCAGA CGATGGAGTC CTTGCCTACA AACTCCTGGT GCAAACCGGC 
AGCCGCGATA AGCCCATCGA CATCAGCCAG TTGACTAAAC AGCGTCTGGT GGATGCAGAT 
GGCATCATTA ATCCCAGCGC TTTCTACATC TACCTGACGG CTTGGGTCAG CAACGACCCC 
GTCGCGTATG CTGCCTCCCA GGCCAACATC CGGCCACACC GACCAGAATG GGTCCACGAC 
AAAGCCGACT ACATGCCTGA AACAAGGCTG AGAATCCCGG CAGCAGAGCC CATCGAGTAT 
GCCCAGTTCC CTTTCTACCT CAACGGCTTG CGGGACACCT CAGACTTTGT GGAGGCAATT 
GAAAAAGTAA GGACCATCTG CAGCAACTAT ACGAGCCTGG GGCTGTCCAG TTACCCCAAC 
GGCTACCCCT TCCTCTTCTG GGAGCAGTAC ATCGGCCTCC GCCACTGGCT GCTGCTGTTC 
ATCAGCGTGG TGTTGGCCTG CACATTCCTC GTGTGCGCTG TCTTCCTTCT GAACCCCTGG 
ACGGCCGGGA TCATTGTGAT GGTCCTGGCG CTGATGACGG TCGAGCTGTT CGGCATGATG 
GGCCTCATCG GAATCAAGCT CAGTGCCGTG CCCGTGGTCA TCCTGATCGC TTCTGTTGGC 
ATAGGAGTGG AGTTCACCGT TCACGTTGCT TTGGCCTTTC TGACGGCCAT CGGCGACAAG 
AACCGCAGGG CTGTGCTTGC CCTGGAGCAC ATGTTTGCAC CCGTCCTGGA TGGCGCCGTG 
TCCACTCTGC TGGGAGTGCT GATGCTGGCG GGATCTGAGT TCGACTTCAT TGTCAGGTAT 
TTCTTTGCTG TGCTGGCGAT CCTCACCATC CTCGGCGTTC TCAATGGGCT GGTTTTGCTT 
CCCGTGCTTT TGTCTTTCTT TGGACCATAT CCTGAGGTGT CTCCAGCCAA CGGCTTGAAC 
CGCCTGCCCA CACCCTCCCC TGAGCCACCC CCCAGCGTGG TCCGCTTCGC CATGCCGCCC 
GGCCACACGC ACAGCGGGTC TGATTCCTCC GACTCGGAGT ATAGTTCCCA GACGACAGTG 
TCAGGCCTCA GCGAGGAGCT TCGGCACTAC GAGGCCCAGC AGGGCGCGGG AGGCCCTGCC 
CACCAAGTGA TCGTGGAAGC CACAGAAAAC CCCGTCTTCG CCCACTCCAC TGTGGTCCAT 
CCCGAATCCA GGCATCACCC ACCCTCGAAC CCGAGACAGC AGCCCCACCT GGACTCAGGG 
TCCCTGCCTC CCGGACGGCA AGGCCAGCAG CCCCGCAGGG ACCCCCCCAG AGAAGGCTTG 
TGGCCACCCC CCTACAGACC GCGCAGAGAC GCTTTTGAAA TTTCTACTGA AGGGCATTCT 
GGCCCTAGCA ATAGGGCCCG CTGGGGCCCT CGCGGGGCCC GTTCTCACAA CCCTCGGAAC 
CCAGCGTCCA CTGCCATGGG CAGCTCCGTG CCCGGCTACT GCCAGCCCAT CACCACTGTG 
ACGGCTTCTG CCTCCGTGAC TGTCGCCGTG CACCCGCCGC CTGTCCCTGG GCCTGGGCGG 
AACCCCCGAG GGGGACTCTG CCCAGGCTAC CCTGAGACTG ACCACGGCCT GTTTGAGGAC 
CCCCACGTGC CTTTCCACGT CCGGTGTGAG AGGAGGGATT CGAAGGTGGA AGTCATTGAG 
CTGCAGGACG TGGAATGCGA GGAGAGGCCC CGGGGAAGCA GCTCCAACTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq

XP_005252159.1

Translation

MFNPQLMIQTPKEEGANVLTTEALLQHLDSALQASRVHVYMYNRQWKLEHLCYKSGELITETGYMDQIIEYLYPCLIITPLDCFWEGAKLQSGTAYLLGKPPLRWTNFDPLEFLEELKKINYQVDSWEEMLNKAEVGHGYMDRPCLNPADPDCPATAPNKNSTKPLDMALVLNGGCHGLSRKYMHWQEELIVGGTVKNSTGKLVSAHALQTMFQLMTPKQMYEHFKGYEYVSHINWNEDKAAAILEAWQRTYVEVVHQSVAQNSTQKVLSFTTTTLDDILKSFSDVSVIRVASGYLLMLAYACLTMLRWDCSKSQGAVGLAGVLLVALSVAAGLGLCSLIGISFNAATTQVLPFLALGVGVDDVFLLAHAFSETGQNKRIPFEDRTGECLKRTGASVALTSISNVTAFFMAALIPIPALRAFSLQAAVVVVFNFAMVLLIFPAILSMDLYRREDRRLDIFCCFTSPCVSRVIQVEPQAYTDTHDNTRYSPPPPYSSHSFAHETQITMQSTVQLRTEYDPHTHVYYTTAEPRSEISVQPVTVTQDTLSCQSPESTSSTRDLLSQFSDSSLHCLEPPCTKWTLSSFAEKHYAPFLLKPKAKVVVIFLFLGLLGVSLYGTTRVRDGLDLTDIVPRETREYDFIAAQFKYFSFYNMYIVTQKADYPNIQHLLYDLHRSFSNVKYVMLEENKQLPKMWLHYFRDWLQGLQDAFDSDWETGKIMPNNYKNGSDDGVLAYKLLVQTGSRDKPIDISQLTKQRLVDADGIINPSAFYIYLTAWVSNDPVAYAASQANIRPHRPEWVHDKADYMPETRLRIPAAEPIEYAQFPFYLNGLRDTSDFVEAIEKVRTICSNYTSLGLSSYPNGYPFLFWEQYIGLRHWLLLFISVVLACTFLVCAVFLLNPWTAGIIVMVLALMTVELFGMMGLIGIKLSAVPVVILIASVGIGVEFTVHVALAFLTAIGDKNRRAVLALEHMFAPVLDGAVSTLLGVLMLAGSEFDFIVRYFFAVLAILTILGVLNGLVLLPVLLSFFGPYPEVSPANGLNRLPTPSPEPPPSVVRFAMPPGHTHSGSDSSDSEYSSQTTVSGLSEELRHYEAQQGAGGPAHQVIVEATENPVFAHSTVVHPESRHHPPSNPRQQPHLDSGSLPPGRQGQQPRRDPPREGLWPPPYRPRRDAFEISTEGHSGPSNRARWGPRGARSHNPRNPASTAMGSSVPGYCQPITTVTASASVTVAVHPPPVPGPGRNPRGGLCPGYPETDHGLFEDPHVPFHVRCERRDSKVEVIELQDVECEERPRGSSSN

Target ORF information:

RefSeq Version	XM_005252102.2
Organism	Homo sapiens(Human)
Definition	Homo sapiens patched 1 (PTCH1), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005252102.2

ORF Insert Sequence:

ATGTTTAATC CTCAACTCAT GATACAGACC CCTAAAGAAG AAGGTGCTAA TGTCCTGACC 
ACAGAAGCGC TCCTACAACA CCTGGACTCG GCACTCCAGG CCAGCCGTGT CCATGTATAC 
ATGTACAACA GGCAGTGGAA ATTGGAACAT TTGTGTTACA AATCAGGAGA GCTTATCACA 
GAAACAGGTT ACATGGATCA GATAATAGAA TATCTTTACC CTTGTTTGAT TATTACACCT 
TTGGACTGCT TCTGGGAAGG GGCGAAATTA CAGTCTGGGA CAGCATACCT CCTAGGTAAA 
CCTCCTTTGC GGTGGACAAA CTTCGACCCT TTGGAATTCC TGGAAGAGTT AAAGAAAATA 
AACTATCAAG TGGACAGCTG GGAGGAAATG CTGAATAAGG CTGAGGTTGG TCATGGTTAC 
ATGGACCGCC CCTGCCTCAA TCCGGCCGAT CCAGACTGCC CCGCCACAGC CCCCAACAAA 
AATTCAACCA AACCTCTTGA TATGGCCCTT GTTTTGAATG GTGGATGTCA TGGCTTATCC 
AGAAAGTATA TGCACTGGCA GGAGGAGTTG ATTGTGGGTG GCACAGTCAA GAACAGCACT 
GGAAAACTCG TCAGCGCCCA TGCCCTGCAG ACCATGTTCC AGTTAATGAC TCCCAAGCAA 
ATGTACGAGC ACTTCAAGGG GTACGAGTAT GTCTCACACA TCAACTGGAA CGAGGACAAA 
GCGGCAGCCA TCCTGGAGGC CTGGCAGAGG ACATATGTGG AGGTGGTTCA TCAGAGTGTC 
GCACAGAACT CCACTCAAAA GGTGCTTTCC TTCACCACCA CGACCCTGGA CGACATCCTG 
AAATCCTTCT CTGACGTCAG TGTCATCCGC GTGGCCAGCG GCTACTTACT CATGCTCGCC 
TATGCCTGTC TAACCATGCT GCGCTGGGAC TGCTCCAAGT CCCAGGGTGC CGTGGGGCTG 
GCTGGCGTCC TGCTGGTTGC ACTGTCAGTG GCTGCAGGAC TGGGCCTGTG CTCATTGATC 
GGAATTTCCT TTAACGCTGC AACAACTCAG GTTTTGCCAT TTCTCGCTCT TGGTGTTGGT 
GTGGATGATG TTTTTCTTCT GGCCCACGCC TTCAGTGAAA CAGGACAGAA TAAAAGAATC 
CCTTTTGAGG ACAGGACCGG GGAGTGCCTG AAGCGCACAG GAGCCAGCGT GGCCCTCACG 
TCCATCAGCA ATGTCACAGC CTTCTTCATG GCCGCGTTAA TCCCAATTCC CGCTCTGCGG 
GCGTTCTCCC TCCAGGCAGC GGTAGTAGTG GTGTTCAATT TTGCCATGGT TCTGCTCATT 
TTTCCTGCAA TTCTCAGCAT GGATTTATAT CGACGCGAGG ACAGGAGACT GGATATTTTC 
TGCTGTTTTA CAAGCCCCTG CGTCAGCAGA GTGATTCAGG TTGAACCTCA GGCCTACACC 
GACACACACG ACAATACCCG CTACAGCCCC CCACCTCCCT ACAGCAGCCA CAGCTTTGCC 
CATGAAACGC AGATTACCAT GCAGTCCACT GTCCAGCTCC GCACGGAGTA CGACCCCCAC 
ACGCACGTGT ACTACACCAC CGCTGAGCCG CGCTCCGAGA TCTCTGTGCA GCCCGTCACC 
GTGACACAGG ACACCCTCAG CTGCCAGAGC CCAGAGAGCA CCAGCTCCAC AAGGGACCTG 
CTCTCCCAGT TCTCCGACTC CAGCCTCCAC TGCCTCGAGC CCCCCTGTAC GAAGTGGACA 
CTCTCATCTT TTGCTGAGAA GCACTATGCT CCTTTCCTCT TGAAACCAAA AGCCAAGGTA 
GTGGTGATCT TCCTTTTTCT GGGCTTGCTG GGGGTCAGCC TTTATGGCAC CACCCGAGTG 
AGAGACGGGC TGGACCTTAC GGACATTGTA CCTCGGGAAA CCAGAGAATA TGACTTTATT 
GCTGCACAAT TCAAATACTT TTCTTTCTAC AACATGTATA TAGTCACCCA GAAAGCAGAC 
TACCCGAATA TCCAGCACTT ACTTTACGAC CTACACAGGA GTTTCAGTAA CGTGAAGTAT 
GTCATGTTGG AAGAAAACAA ACAGCTTCCC AAAATGTGGC TGCACTACTT CAGAGACTGG 
CTTCAGGGAC TTCAGGATGC ATTTGACAGT GACTGGGAAA CCGGGAAAAT CATGCCAAAC 
AATTACAAGA ATGGATCAGA CGATGGAGTC CTTGCCTACA AACTCCTGGT GCAAACCGGC 
AGCCGCGATA AGCCCATCGA CATCAGCCAG TTGACTAAAC AGCGTCTGGT GGATGCAGAT 
GGCATCATTA ATCCCAGCGC TTTCTACATC TACCTGACGG CTTGGGTCAG CAACGACCCC 
GTCGCGTATG CTGCCTCCCA GGCCAACATC CGGCCACACC GACCAGAATG GGTCCACGAC 
AAAGCCGACT ACATGCCTGA AACAAGGCTG AGAATCCCGG CAGCAGAGCC CATCGAGTAT 
GCCCAGTTCC CTTTCTACCT CAACGGCTTG CGGGACACCT CAGACTTTGT GGAGGCAATT 
GAAAAAGTAA GGACCATCTG CAGCAACTAT ACGAGCCTGG GGCTGTCCAG TTACCCCAAC 
GGCTACCCCT TCCTCTTCTG GGAGCAGTAC ATCGGCCTCC GCCACTGGCT GCTGCTGTTC 
ATCAGCGTGG TGTTGGCCTG CACATTCCTC GTGTGCGCTG TCTTCCTTCT GAACCCCTGG 
ACGGCCGGGA TCATTGTGAT GGTCCTGGCG CTGATGACGG TCGAGCTGTT CGGCATGATG 
GGCCTCATCG GAATCAAGCT CAGTGCCGTG CCCGTGGTCA TCCTGATCGC TTCTGTTGGC 
ATAGGAGTGG AGTTCACCGT TCACGTTGCT TTGGCCTTTC TGACGGCCAT CGGCGACAAG 
AACCGCAGGG CTGTGCTTGC CCTGGAGCAC ATGTTTGCAC CCGTCCTGGA TGGCGCCGTG 
TCCACTCTGC TGGGAGTGCT GATGCTGGCG GGATCTGAGT TCGACTTCAT TGTCAGGTAT 
TTCTTTGCTG TGCTGGCGAT CCTCACCATC CTCGGCGTTC TCAATGGGCT GGTTTTGCTT 
CCCGTGCTTT TGTCTTTCTT TGGACCATAT CCTGAGGTGT CTCCAGCCAA CGGCTTGAAC 
CGCCTGCCCA CACCCTCCCC TGAGCCACCC CCCAGCGTGG TCCGCTTCGC CATGCCGCCC 
GGCCACACGC ACAGCGGGTC TGATTCCTCC GACTCGGAGT ATAGTTCCCA GACGACAGTG 
TCAGGCCTCA GCGAGGAGCT TCGGCACTAC GAGGCCCAGC AGGGCGCGGG AGGCCCTGCC 
CACCAAGTGA TCGTGGAAGC CACAGAAAAC CCCGTCTTCG CCCACTCCAC TGTGGTCCAT 
CCCGAATCCA GGCATCACCC ACCCTCGAAC CCGAGACAGC AGCCCCACCT GGACTCAGGG 
TCCCTGCCTC CCGGACGGCA AGGCCAGCAG CCCCGCAGGG ACCCCCCCAG AGAAGGCTTG 
TGGCCACCCC CCTACAGACC GCGCAGAGAC GCTTTTGAAA TTTCTACTGA AGGGCATTCT 
GGCCCTAGCA ATAGGGCCCG CTGGGGCCCT CGCGGGGCCC GTTCTCACAA CCCTCGGAAC 
CCAGCGTCCA CTGCCATGGG CAGCTCCGTG CCCGGCTACT GCCAGCCCAT CACCACTGTG 
ACGGCTTCTG CCTCCGTGAC TGTCGCCGTG CACCCGCCGC CTGTCCCTGG GCCTGGGCGG 
AACCCCCGAG GGGGACTCTG CCCAGGCTAC CCTGAGACTG ACCACGGCCT GTTTGAGGAC 
CCCCACGTGC CTTTCCACGT CCGGTGTGAG AGGAGGGATT CGAAGGTGGA AGTCATTGAG 
CTGCAGGACG TGGAATGCGA GGAGAGGCCC CGGGGAAGCA GCTCCAACTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PTCH1 ( XM_011518872.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_011517174.1 Homo sapiens patched 1 (PTCH1), transcript variant X6, mRNA.

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CloneID	OHu17265
Clone ID Related Accession (Same CDS sequence)	XM_005252102.2 , XM_011518872.1 , NM_001083605.1 , NM_001083604.2 , NM_001083606.2 , NM_001083606.1 , XM_011518871.1 , XM_011518869.1 , NM_001083604.1 , XM_011518870.1 , NM_001083607.1 , NM_001083606.3 , NM_001083605.2 , NM_001083607.2
Accession Version	XM_011518872.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3891bp) Protein sequence SNP
Vector	pcDNA3.1⁺/C-(K)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-03-11
Organism	Homo sapiens(Human)
Product	protein patched homolog 1 isoform X1
Comment

ATGTTTAATC CTCAACTCAT GATACAGACC CCTAAAGAAG AAGGTGCTAA TGTCCTGACC 
ACAGAAGCGC TCCTACAACA CCTGGACTCG GCACTCCAGG CCAGCCGTGT CCATGTATAC 
ATGTACAACA GGCAGTGGAA ATTGGAACAT TTGTGTTACA AATCAGGAGA GCTTATCACA 
GAAACAGGTT ACATGGATCA GATAATAGAA TATCTTTACC CTTGTTTGAT TATTACACCT 
TTGGACTGCT TCTGGGAAGG GGCGAAATTA CAGTCTGGGA CAGCATACCT CCTAGGTAAA 
CCTCCTTTGC GGTGGACAAA CTTCGACCCT TTGGAATTCC TGGAAGAGTT AAAGAAAATA 
AACTATCAAG TGGACAGCTG GGAGGAAATG CTGAATAAGG CTGAGGTTGG TCATGGTTAC 
ATGGACCGCC CCTGCCTCAA TCCGGCCGAT CCAGACTGCC CCGCCACAGC CCCCAACAAA 
AATTCAACCA AACCTCTTGA TATGGCCCTT GTTTTGAATG GTGGATGTCA TGGCTTATCC 
AGAAAGTATA TGCACTGGCA GGAGGAGTTG ATTGTGGGTG GCACAGTCAA GAACAGCACT 
GGAAAACTCG TCAGCGCCCA TGCCCTGCAG ACCATGTTCC AGTTAATGAC TCCCAAGCAA 
ATGTACGAGC ACTTCAAGGG GTACGAGTAT GTCTCACACA TCAACTGGAA CGAGGACAAA 
GCGGCAGCCA TCCTGGAGGC CTGGCAGAGG ACATATGTGG AGGTGGTTCA TCAGAGTGTC 
GCACAGAACT CCACTCAAAA GGTGCTTTCC TTCACCACCA CGACCCTGGA CGACATCCTG 
AAATCCTTCT CTGACGTCAG TGTCATCCGC GTGGCCAGCG GCTACTTACT CATGCTCGCC 
TATGCCTGTC TAACCATGCT GCGCTGGGAC TGCTCCAAGT CCCAGGGTGC CGTGGGGCTG 
GCTGGCGTCC TGCTGGTTGC ACTGTCAGTG GCTGCAGGAC TGGGCCTGTG CTCATTGATC 
GGAATTTCCT TTAACGCTGC AACAACTCAG GTTTTGCCAT TTCTCGCTCT TGGTGTTGGT 
GTGGATGATG TTTTTCTTCT GGCCCACGCC TTCAGTGAAA CAGGACAGAA TAAAAGAATC 
CCTTTTGAGG ACAGGACCGG GGAGTGCCTG AAGCGCACAG GAGCCAGCGT GGCCCTCACG 
TCCATCAGCA ATGTCACAGC CTTCTTCATG GCCGCGTTAA TCCCAATTCC CGCTCTGCGG 
GCGTTCTCCC TCCAGGCAGC GGTAGTAGTG GTGTTCAATT TTGCCATGGT TCTGCTCATT 
TTTCCTGCAA TTCTCAGCAT GGATTTATAT CGACGCGAGG ACAGGAGACT GGATATTTTC 
TGCTGTTTTA CAAGCCCCTG CGTCAGCAGA GTGATTCAGG TTGAACCTCA GGCCTACACC 
GACACACACG ACAATACCCG CTACAGCCCC CCACCTCCCT ACAGCAGCCA CAGCTTTGCC 
CATGAAACGC AGATTACCAT GCAGTCCACT GTCCAGCTCC GCACGGAGTA CGACCCCCAC 
ACGCACGTGT ACTACACCAC CGCTGAGCCG CGCTCCGAGA TCTCTGTGCA GCCCGTCACC 
GTGACACAGG ACACCCTCAG CTGCCAGAGC CCAGAGAGCA CCAGCTCCAC AAGGGACCTG 
CTCTCCCAGT TCTCCGACTC CAGCCTCCAC TGCCTCGAGC CCCCCTGTAC GAAGTGGACA 
CTCTCATCTT TTGCTGAGAA GCACTATGCT CCTTTCCTCT TGAAACCAAA AGCCAAGGTA 
GTGGTGATCT TCCTTTTTCT GGGCTTGCTG GGGGTCAGCC TTTATGGCAC CACCCGAGTG 
AGAGACGGGC TGGACCTTAC GGACATTGTA CCTCGGGAAA CCAGAGAATA TGACTTTATT 
GCTGCACAAT TCAAATACTT TTCTTTCTAC AACATGTATA TAGTCACCCA GAAAGCAGAC 
TACCCGAATA TCCAGCACTT ACTTTACGAC CTACACAGGA GTTTCAGTAA CGTGAAGTAT 
GTCATGTTGG AAGAAAACAA ACAGCTTCCC AAAATGTGGC TGCACTACTT CAGAGACTGG 
CTTCAGGGAC TTCAGGATGC ATTTGACAGT GACTGGGAAA CCGGGAAAAT CATGCCAAAC 
AATTACAAGA ATGGATCAGA CGATGGAGTC CTTGCCTACA AACTCCTGGT GCAAACCGGC 
AGCCGCGATA AGCCCATCGA CATCAGCCAG TTGACTAAAC AGCGTCTGGT GGATGCAGAT 
GGCATCATTA ATCCCAGCGC TTTCTACATC TACCTGACGG CTTGGGTCAG CAACGACCCC 
GTCGCGTATG CTGCCTCCCA GGCCAACATC CGGCCACACC GACCAGAATG GGTCCACGAC 
AAAGCCGACT ACATGCCTGA AACAAGGCTG AGAATCCCGG CAGCAGAGCC CATCGAGTAT 
GCCCAGTTCC CTTTCTACCT CAACGGCTTG CGGGACACCT CAGACTTTGT GGAGGCAATT 
GAAAAAGTAA GGACCATCTG CAGCAACTAT ACGAGCCTGG GGCTGTCCAG TTACCCCAAC 
GGCTACCCCT TCCTCTTCTG GGAGCAGTAC ATCGGCCTCC GCCACTGGCT GCTGCTGTTC 
ATCAGCGTGG TGTTGGCCTG CACATTCCTC GTGTGCGCTG TCTTCCTTCT GAACCCCTGG 
ACGGCCGGGA TCATTGTGAT GGTCCTGGCG CTGATGACGG TCGAGCTGTT CGGCATGATG 
GGCCTCATCG GAATCAAGCT CAGTGCCGTG CCCGTGGTCA TCCTGATCGC TTCTGTTGGC 
ATAGGAGTGG AGTTCACCGT TCACGTTGCT TTGGCCTTTC TGACGGCCAT CGGCGACAAG 
AACCGCAGGG CTGTGCTTGC CCTGGAGCAC ATGTTTGCAC CCGTCCTGGA TGGCGCCGTG 
TCCACTCTGC TGGGAGTGCT GATGCTGGCG GGATCTGAGT TCGACTTCAT TGTCAGGTAT 
TTCTTTGCTG TGCTGGCGAT CCTCACCATC CTCGGCGTTC TCAATGGGCT GGTTTTGCTT 
CCCGTGCTTT TGTCTTTCTT TGGACCATAT CCTGAGGTGT CTCCAGCCAA CGGCTTGAAC 
CGCCTGCCCA CACCCTCCCC TGAGCCACCC CCCAGCGTGG TCCGCTTCGC CATGCCGCCC 
GGCCACACGC ACAGCGGGTC TGATTCCTCC GACTCGGAGT ATAGTTCCCA GACGACAGTG 
TCAGGCCTCA GCGAGGAGCT TCGGCACTAC GAGGCCCAGC AGGGCGCGGG AGGCCCTGCC 
CACCAAGTGA TCGTGGAAGC CACAGAAAAC CCCGTCTTCG CCCACTCCAC TGTGGTCCAT 
CCCGAATCCA GGCATCACCC ACCCTCGAAC CCGAGACAGC AGCCCCACCT GGACTCAGGG 
TCCCTGCCTC CCGGACGGCA AGGCCAGCAG CCCCGCAGGG ACCCCCCCAG AGAAGGCTTG 
TGGCCACCCC CCTACAGACC GCGCAGAGAC GCTTTTGAAA TTTCTACTGA AGGGCATTCT 
GGCCCTAGCA ATAGGGCCCG CTGGGGCCCT CGCGGGGCCC GTTCTCACAA CCCTCGGAAC 
CCAGCGTCCA CTGCCATGGG CAGCTCCGTG CCCGGCTACT GCCAGCCCAT CACCACTGTG 
ACGGCTTCTG CCTCCGTGAC TGTCGCCGTG CACCCGCCGC CTGTCCCTGG GCCTGGGCGG 
AACCCCCGAG GGGGACTCTG CCCAGGCTAC CCTGAGACTG ACCACGGCCT GTTTGAGGAC 
CCCCACGTGC CTTTCCACGT CCGGTGTGAG AGGAGGGATT CGAAGGTGGA AGTCATTGAG 
CTGCAGGACG TGGAATGCGA GGAGAGGCCC CGGGGAAGCA GCTCCAACTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq

XP_011517174.1

Translation

MFNPQLMIQTPKEEGANVLTTEALLQHLDSALQASRVHVYMYNRQWKLEHLCYKSGELITETGYMDQIIEYLYPCLIITPLDCFWEGAKLQSGTAYLLGKPPLRWTNFDPLEFLEELKKINYQVDSWEEMLNKAEVGHGYMDRPCLNPADPDCPATAPNKNSTKPLDMALVLNGGCHGLSRKYMHWQEELIVGGTVKNSTGKLVSAHALQTMFQLMTPKQMYEHFKGYEYVSHINWNEDKAAAILEAWQRTYVEVVHQSVAQNSTQKVLSFTTTTLDDILKSFSDVSVIRVASGYLLMLAYACLTMLRWDCSKSQGAVGLAGVLLVALSVAAGLGLCSLIGISFNAATTQVLPFLALGVGVDDVFLLAHAFSETGQNKRIPFEDRTGECLKRTGASVALTSISNVTAFFMAALIPIPALRAFSLQAAVVVVFNFAMVLLIFPAILSMDLYRREDRRLDIFCCFTSPCVSRVIQVEPQAYTDTHDNTRYSPPPPYSSHSFAHETQITMQSTVQLRTEYDPHTHVYYTTAEPRSEISVQPVTVTQDTLSCQSPESTSSTRDLLSQFSDSSLHCLEPPCTKWTLSSFAEKHYAPFLLKPKAKVVVIFLFLGLLGVSLYGTTRVRDGLDLTDIVPRETREYDFIAAQFKYFSFYNMYIVTQKADYPNIQHLLYDLHRSFSNVKYVMLEENKQLPKMWLHYFRDWLQGLQDAFDSDWETGKIMPNNYKNGSDDGVLAYKLLVQTGSRDKPIDISQLTKQRLVDADGIINPSAFYIYLTAWVSNDPVAYAASQANIRPHRPEWVHDKADYMPETRLRIPAAEPIEYAQFPFYLNGLRDTSDFVEAIEKVRTICSNYTSLGLSSYPNGYPFLFWEQYIGLRHWLLLFISVVLACTFLVCAVFLLNPWTAGIIVMVLALMTVELFGMMGLIGIKLSAVPVVILIASVGIGVEFTVHVALAFLTAIGDKNRRAVLALEHMFAPVLDGAVSTLLGVLMLAGSEFDFIVRYFFAVLAILTILGVLNGLVLLPVLLSFFGPYPEVSPANGLNRLPTPSPEPPPSVVRFAMPPGHTHSGSDSSDSEYSSQTTVSGLSEELRHYEAQQGAGGPAHQVIVEATENPVFAHSTVVHPESRHHPPSNPRQQPHLDSGSLPPGRQGQQPRRDPPREGLWPPPYRPRRDAFEISTEGHSGPSNRARWGPRGARSHNPRNPASTAMGSSVPGYCQPITTVTASASVTVAVHPPPVPGPGRNPRGGLCPGYPETDHGLFEDPHVPFHVRCERRDSKVEVIELQDVECEERPRGSSSN

Target ORF information:

RefSeq Version	XM_011518872.1
Organism	Homo sapiens(Human)
Definition	Homo sapiens patched 1 (PTCH1), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011518872.1

ORF Insert Sequence:

ATGTTTAATC CTCAACTCAT GATACAGACC CCTAAAGAAG AAGGTGCTAA TGTCCTGACC 
ACAGAAGCGC TCCTACAACA CCTGGACTCG GCACTCCAGG CCAGCCGTGT CCATGTATAC 
ATGTACAACA GGCAGTGGAA ATTGGAACAT TTGTGTTACA AATCAGGAGA GCTTATCACA 
GAAACAGGTT ACATGGATCA GATAATAGAA TATCTTTACC CTTGTTTGAT TATTACACCT 
TTGGACTGCT TCTGGGAAGG GGCGAAATTA CAGTCTGGGA CAGCATACCT CCTAGGTAAA 
CCTCCTTTGC GGTGGACAAA CTTCGACCCT TTGGAATTCC TGGAAGAGTT AAAGAAAATA 
AACTATCAAG TGGACAGCTG GGAGGAAATG CTGAATAAGG CTGAGGTTGG TCATGGTTAC 
ATGGACCGCC CCTGCCTCAA TCCGGCCGAT CCAGACTGCC CCGCCACAGC CCCCAACAAA 
AATTCAACCA AACCTCTTGA TATGGCCCTT GTTTTGAATG GTGGATGTCA TGGCTTATCC 
AGAAAGTATA TGCACTGGCA GGAGGAGTTG ATTGTGGGTG GCACAGTCAA GAACAGCACT 
GGAAAACTCG TCAGCGCCCA TGCCCTGCAG ACCATGTTCC AGTTAATGAC TCCCAAGCAA 
ATGTACGAGC ACTTCAAGGG GTACGAGTAT GTCTCACACA TCAACTGGAA CGAGGACAAA 
GCGGCAGCCA TCCTGGAGGC CTGGCAGAGG ACATATGTGG AGGTGGTTCA TCAGAGTGTC 
GCACAGAACT CCACTCAAAA GGTGCTTTCC TTCACCACCA CGACCCTGGA CGACATCCTG 
AAATCCTTCT CTGACGTCAG TGTCATCCGC GTGGCCAGCG GCTACTTACT CATGCTCGCC 
TATGCCTGTC TAACCATGCT GCGCTGGGAC TGCTCCAAGT CCCAGGGTGC CGTGGGGCTG 
GCTGGCGTCC TGCTGGTTGC ACTGTCAGTG GCTGCAGGAC TGGGCCTGTG CTCATTGATC 
GGAATTTCCT TTAACGCTGC AACAACTCAG GTTTTGCCAT TTCTCGCTCT TGGTGTTGGT 
GTGGATGATG TTTTTCTTCT GGCCCACGCC TTCAGTGAAA CAGGACAGAA TAAAAGAATC 
CCTTTTGAGG ACAGGACCGG GGAGTGCCTG AAGCGCACAG GAGCCAGCGT GGCCCTCACG 
TCCATCAGCA ATGTCACAGC CTTCTTCATG GCCGCGTTAA TCCCAATTCC CGCTCTGCGG 
GCGTTCTCCC TCCAGGCAGC GGTAGTAGTG GTGTTCAATT TTGCCATGGT TCTGCTCATT 
TTTCCTGCAA TTCTCAGCAT GGATTTATAT CGACGCGAGG ACAGGAGACT GGATATTTTC 
TGCTGTTTTA CAAGCCCCTG CGTCAGCAGA GTGATTCAGG TTGAACCTCA GGCCTACACC 
GACACACACG ACAATACCCG CTACAGCCCC CCACCTCCCT ACAGCAGCCA CAGCTTTGCC 
CATGAAACGC AGATTACCAT GCAGTCCACT GTCCAGCTCC GCACGGAGTA CGACCCCCAC 
ACGCACGTGT ACTACACCAC CGCTGAGCCG CGCTCCGAGA TCTCTGTGCA GCCCGTCACC 
GTGACACAGG ACACCCTCAG CTGCCAGAGC CCAGAGAGCA CCAGCTCCAC AAGGGACCTG 
CTCTCCCAGT TCTCCGACTC CAGCCTCCAC TGCCTCGAGC CCCCCTGTAC GAAGTGGACA 
CTCTCATCTT TTGCTGAGAA GCACTATGCT CCTTTCCTCT TGAAACCAAA AGCCAAGGTA 
GTGGTGATCT TCCTTTTTCT GGGCTTGCTG GGGGTCAGCC TTTATGGCAC CACCCGAGTG 
AGAGACGGGC TGGACCTTAC GGACATTGTA CCTCGGGAAA CCAGAGAATA TGACTTTATT 
GCTGCACAAT TCAAATACTT TTCTTTCTAC AACATGTATA TAGTCACCCA GAAAGCAGAC 
TACCCGAATA TCCAGCACTT ACTTTACGAC CTACACAGGA GTTTCAGTAA CGTGAAGTAT 
GTCATGTTGG AAGAAAACAA ACAGCTTCCC AAAATGTGGC TGCACTACTT CAGAGACTGG 
CTTCAGGGAC TTCAGGATGC ATTTGACAGT GACTGGGAAA CCGGGAAAAT CATGCCAAAC 
AATTACAAGA ATGGATCAGA CGATGGAGTC CTTGCCTACA AACTCCTGGT GCAAACCGGC 
AGCCGCGATA AGCCCATCGA CATCAGCCAG TTGACTAAAC AGCGTCTGGT GGATGCAGAT 
GGCATCATTA ATCCCAGCGC TTTCTACATC TACCTGACGG CTTGGGTCAG CAACGACCCC 
GTCGCGTATG CTGCCTCCCA GGCCAACATC CGGCCACACC GACCAGAATG GGTCCACGAC 
AAAGCCGACT ACATGCCTGA AACAAGGCTG AGAATCCCGG CAGCAGAGCC CATCGAGTAT 
GCCCAGTTCC CTTTCTACCT CAACGGCTTG CGGGACACCT CAGACTTTGT GGAGGCAATT 
GAAAAAGTAA GGACCATCTG CAGCAACTAT ACGAGCCTGG GGCTGTCCAG TTACCCCAAC 
GGCTACCCCT TCCTCTTCTG GGAGCAGTAC ATCGGCCTCC GCCACTGGCT GCTGCTGTTC 
ATCAGCGTGG TGTTGGCCTG CACATTCCTC GTGTGCGCTG TCTTCCTTCT GAACCCCTGG 
ACGGCCGGGA TCATTGTGAT GGTCCTGGCG CTGATGACGG TCGAGCTGTT CGGCATGATG 
GGCCTCATCG GAATCAAGCT CAGTGCCGTG CCCGTGGTCA TCCTGATCGC TTCTGTTGGC 
ATAGGAGTGG AGTTCACCGT TCACGTTGCT TTGGCCTTTC TGACGGCCAT CGGCGACAAG 
AACCGCAGGG CTGTGCTTGC CCTGGAGCAC ATGTTTGCAC CCGTCCTGGA TGGCGCCGTG 
TCCACTCTGC TGGGAGTGCT GATGCTGGCG GGATCTGAGT TCGACTTCAT TGTCAGGTAT 
TTCTTTGCTG TGCTGGCGAT CCTCACCATC CTCGGCGTTC TCAATGGGCT GGTTTTGCTT 
CCCGTGCTTT TGTCTTTCTT TGGACCATAT CCTGAGGTGT CTCCAGCCAA CGGCTTGAAC 
CGCCTGCCCA CACCCTCCCC TGAGCCACCC CCCAGCGTGG TCCGCTTCGC CATGCCGCCC 
GGCCACACGC ACAGCGGGTC TGATTCCTCC GACTCGGAGT ATAGTTCCCA GACGACAGTG 
TCAGGCCTCA GCGAGGAGCT TCGGCACTAC GAGGCCCAGC AGGGCGCGGG AGGCCCTGCC 
CACCAAGTGA TCGTGGAAGC CACAGAAAAC CCCGTCTTCG CCCACTCCAC TGTGGTCCAT 
CCCGAATCCA GGCATCACCC ACCCTCGAAC CCGAGACAGC AGCCCCACCT GGACTCAGGG 
TCCCTGCCTC CCGGACGGCA AGGCCAGCAG CCCCGCAGGG ACCCCCCCAG AGAAGGCTTG 
TGGCCACCCC CCTACAGACC GCGCAGAGAC GCTTTTGAAA TTTCTACTGA AGGGCATTCT 
GGCCCTAGCA ATAGGGCCCG CTGGGGCCCT CGCGGGGCCC GTTCTCACAA CCCTCGGAAC 
CCAGCGTCCA CTGCCATGGG CAGCTCCGTG CCCGGCTACT GCCAGCCCAT CACCACTGTG 
ACGGCTTCTG CCTCCGTGAC TGTCGCCGTG CACCCGCCGC CTGTCCCTGG GCCTGGGCGG 
AACCCCCGAG GGGGACTCTG CCCAGGCTAC CCTGAGACTG ACCACGGCCT GTTTGAGGAC 
CCCCACGTGC CTTTCCACGT CCGGTGTGAG AGGAGGGATT CGAAGGTGGA AGTCATTGAG 
CTGCAGGACG TGGAATGCGA GGAGAGGCCC CGGGGAAGCA GCTCCAACTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PTCH1 ( XM_011518871.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_011517173.1 Homo sapiens patched 1 (PTCH1), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OHu17265
Clone ID Related Accession (Same CDS sequence)	XM_005252102.2 , XM_011518872.1 , NM_001083605.1 , NM_001083604.2 , NM_001083606.2 , NM_001083606.1 , XM_011518871.1 , XM_011518869.1 , NM_001083604.1 , XM_011518870.1 , NM_001083607.1 , NM_001083606.3 , NM_001083605.2 , NM_001083607.2
Accession Version	XM_011518871.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3891bp) Protein sequence SNP
Vector	pcDNA3.1⁺/C-(K)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-03-11
Organism	Homo sapiens(Human)
Product	protein patched homolog 1 isoform X1
Comment

ATGTTTAATC CTCAACTCAT GATACAGACC CCTAAAGAAG AAGGTGCTAA TGTCCTGACC 
ACAGAAGCGC TCCTACAACA CCTGGACTCG GCACTCCAGG CCAGCCGTGT CCATGTATAC 
ATGTACAACA GGCAGTGGAA ATTGGAACAT TTGTGTTACA AATCAGGAGA GCTTATCACA 
GAAACAGGTT ACATGGATCA GATAATAGAA TATCTTTACC CTTGTTTGAT TATTACACCT 
TTGGACTGCT TCTGGGAAGG GGCGAAATTA CAGTCTGGGA CAGCATACCT CCTAGGTAAA 
CCTCCTTTGC GGTGGACAAA CTTCGACCCT TTGGAATTCC TGGAAGAGTT AAAGAAAATA 
AACTATCAAG TGGACAGCTG GGAGGAAATG CTGAATAAGG CTGAGGTTGG TCATGGTTAC 
ATGGACCGCC CCTGCCTCAA TCCGGCCGAT CCAGACTGCC CCGCCACAGC CCCCAACAAA 
AATTCAACCA AACCTCTTGA TATGGCCCTT GTTTTGAATG GTGGATGTCA TGGCTTATCC 
AGAAAGTATA TGCACTGGCA GGAGGAGTTG ATTGTGGGTG GCACAGTCAA GAACAGCACT 
GGAAAACTCG TCAGCGCCCA TGCCCTGCAG ACCATGTTCC AGTTAATGAC TCCCAAGCAA 
ATGTACGAGC ACTTCAAGGG GTACGAGTAT GTCTCACACA TCAACTGGAA CGAGGACAAA 
GCGGCAGCCA TCCTGGAGGC CTGGCAGAGG ACATATGTGG AGGTGGTTCA TCAGAGTGTC 
GCACAGAACT CCACTCAAAA GGTGCTTTCC TTCACCACCA CGACCCTGGA CGACATCCTG 
AAATCCTTCT CTGACGTCAG TGTCATCCGC GTGGCCAGCG GCTACTTACT CATGCTCGCC 
TATGCCTGTC TAACCATGCT GCGCTGGGAC TGCTCCAAGT CCCAGGGTGC CGTGGGGCTG 
GCTGGCGTCC TGCTGGTTGC ACTGTCAGTG GCTGCAGGAC TGGGCCTGTG CTCATTGATC 
GGAATTTCCT TTAACGCTGC AACAACTCAG GTTTTGCCAT TTCTCGCTCT TGGTGTTGGT 
GTGGATGATG TTTTTCTTCT GGCCCACGCC TTCAGTGAAA CAGGACAGAA TAAAAGAATC 
CCTTTTGAGG ACAGGACCGG GGAGTGCCTG AAGCGCACAG GAGCCAGCGT GGCCCTCACG 
TCCATCAGCA ATGTCACAGC CTTCTTCATG GCCGCGTTAA TCCCAATTCC CGCTCTGCGG 
GCGTTCTCCC TCCAGGCAGC GGTAGTAGTG GTGTTCAATT TTGCCATGGT TCTGCTCATT 
TTTCCTGCAA TTCTCAGCAT GGATTTATAT CGACGCGAGG ACAGGAGACT GGATATTTTC 
TGCTGTTTTA CAAGCCCCTG CGTCAGCAGA GTGATTCAGG TTGAACCTCA GGCCTACACC 
GACACACACG ACAATACCCG CTACAGCCCC CCACCTCCCT ACAGCAGCCA CAGCTTTGCC 
CATGAAACGC AGATTACCAT GCAGTCCACT GTCCAGCTCC GCACGGAGTA CGACCCCCAC 
ACGCACGTGT ACTACACCAC CGCTGAGCCG CGCTCCGAGA TCTCTGTGCA GCCCGTCACC 
GTGACACAGG ACACCCTCAG CTGCCAGAGC CCAGAGAGCA CCAGCTCCAC AAGGGACCTG 
CTCTCCCAGT TCTCCGACTC CAGCCTCCAC TGCCTCGAGC CCCCCTGTAC GAAGTGGACA 
CTCTCATCTT TTGCTGAGAA GCACTATGCT CCTTTCCTCT TGAAACCAAA AGCCAAGGTA 
GTGGTGATCT TCCTTTTTCT GGGCTTGCTG GGGGTCAGCC TTTATGGCAC CACCCGAGTG 
AGAGACGGGC TGGACCTTAC GGACATTGTA CCTCGGGAAA CCAGAGAATA TGACTTTATT 
GCTGCACAAT TCAAATACTT TTCTTTCTAC AACATGTATA TAGTCACCCA GAAAGCAGAC 
TACCCGAATA TCCAGCACTT ACTTTACGAC CTACACAGGA GTTTCAGTAA CGTGAAGTAT 
GTCATGTTGG AAGAAAACAA ACAGCTTCCC AAAATGTGGC TGCACTACTT CAGAGACTGG 
CTTCAGGGAC TTCAGGATGC ATTTGACAGT GACTGGGAAA CCGGGAAAAT CATGCCAAAC 
AATTACAAGA ATGGATCAGA CGATGGAGTC CTTGCCTACA AACTCCTGGT GCAAACCGGC 
AGCCGCGATA AGCCCATCGA CATCAGCCAG TTGACTAAAC AGCGTCTGGT GGATGCAGAT 
GGCATCATTA ATCCCAGCGC TTTCTACATC TACCTGACGG CTTGGGTCAG CAACGACCCC 
GTCGCGTATG CTGCCTCCCA GGCCAACATC CGGCCACACC GACCAGAATG GGTCCACGAC 
AAAGCCGACT ACATGCCTGA AACAAGGCTG AGAATCCCGG CAGCAGAGCC CATCGAGTAT 
GCCCAGTTCC CTTTCTACCT CAACGGCTTG CGGGACACCT CAGACTTTGT GGAGGCAATT 
GAAAAAGTAA GGACCATCTG CAGCAACTAT ACGAGCCTGG GGCTGTCCAG TTACCCCAAC 
GGCTACCCCT TCCTCTTCTG GGAGCAGTAC ATCGGCCTCC GCCACTGGCT GCTGCTGTTC 
ATCAGCGTGG TGTTGGCCTG CACATTCCTC GTGTGCGCTG TCTTCCTTCT GAACCCCTGG 
ACGGCCGGGA TCATTGTGAT GGTCCTGGCG CTGATGACGG TCGAGCTGTT CGGCATGATG 
GGCCTCATCG GAATCAAGCT CAGTGCCGTG CCCGTGGTCA TCCTGATCGC TTCTGTTGGC 
ATAGGAGTGG AGTTCACCGT TCACGTTGCT TTGGCCTTTC TGACGGCCAT CGGCGACAAG 
AACCGCAGGG CTGTGCTTGC CCTGGAGCAC ATGTTTGCAC CCGTCCTGGA TGGCGCCGTG 
TCCACTCTGC TGGGAGTGCT GATGCTGGCG GGATCTGAGT TCGACTTCAT TGTCAGGTAT 
TTCTTTGCTG TGCTGGCGAT CCTCACCATC CTCGGCGTTC TCAATGGGCT GGTTTTGCTT 
CCCGTGCTTT TGTCTTTCTT TGGACCATAT CCTGAGGTGT CTCCAGCCAA CGGCTTGAAC 
CGCCTGCCCA CACCCTCCCC TGAGCCACCC CCCAGCGTGG TCCGCTTCGC CATGCCGCCC 
GGCCACACGC ACAGCGGGTC TGATTCCTCC GACTCGGAGT ATAGTTCCCA GACGACAGTG 
TCAGGCCTCA GCGAGGAGCT TCGGCACTAC GAGGCCCAGC AGGGCGCGGG AGGCCCTGCC 
CACCAAGTGA TCGTGGAAGC CACAGAAAAC CCCGTCTTCG CCCACTCCAC TGTGGTCCAT 
CCCGAATCCA GGCATCACCC ACCCTCGAAC CCGAGACAGC AGCCCCACCT GGACTCAGGG 
TCCCTGCCTC CCGGACGGCA AGGCCAGCAG CCCCGCAGGG ACCCCCCCAG AGAAGGCTTG 
TGGCCACCCC CCTACAGACC GCGCAGAGAC GCTTTTGAAA TTTCTACTGA AGGGCATTCT 
GGCCCTAGCA ATAGGGCCCG CTGGGGCCCT CGCGGGGCCC GTTCTCACAA CCCTCGGAAC 
CCAGCGTCCA CTGCCATGGG CAGCTCCGTG CCCGGCTACT GCCAGCCCAT CACCACTGTG 
ACGGCTTCTG CCTCCGTGAC TGTCGCCGTG CACCCGCCGC CTGTCCCTGG GCCTGGGCGG 
AACCCCCGAG GGGGACTCTG CCCAGGCTAC CCTGAGACTG ACCACGGCCT GTTTGAGGAC 
CCCCACGTGC CTTTCCACGT CCGGTGTGAG AGGAGGGATT CGAAGGTGGA AGTCATTGAG 
CTGCAGGACG TGGAATGCGA GGAGAGGCCC CGGGGAAGCA GCTCCAACTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq

XP_011517173.1

Translation

MFNPQLMIQTPKEEGANVLTTEALLQHLDSALQASRVHVYMYNRQWKLEHLCYKSGELITETGYMDQIIEYLYPCLIITPLDCFWEGAKLQSGTAYLLGKPPLRWTNFDPLEFLEELKKINYQVDSWEEMLNKAEVGHGYMDRPCLNPADPDCPATAPNKNSTKPLDMALVLNGGCHGLSRKYMHWQEELIVGGTVKNSTGKLVSAHALQTMFQLMTPKQMYEHFKGYEYVSHINWNEDKAAAILEAWQRTYVEVVHQSVAQNSTQKVLSFTTTTLDDILKSFSDVSVIRVASGYLLMLAYACLTMLRWDCSKSQGAVGLAGVLLVALSVAAGLGLCSLIGISFNAATTQVLPFLALGVGVDDVFLLAHAFSETGQNKRIPFEDRTGECLKRTGASVALTSISNVTAFFMAALIPIPALRAFSLQAAVVVVFNFAMVLLIFPAILSMDLYRREDRRLDIFCCFTSPCVSRVIQVEPQAYTDTHDNTRYSPPPPYSSHSFAHETQITMQSTVQLRTEYDPHTHVYYTTAEPRSEISVQPVTVTQDTLSCQSPESTSSTRDLLSQFSDSSLHCLEPPCTKWTLSSFAEKHYAPFLLKPKAKVVVIFLFLGLLGVSLYGTTRVRDGLDLTDIVPRETREYDFIAAQFKYFSFYNMYIVTQKADYPNIQHLLYDLHRSFSNVKYVMLEENKQLPKMWLHYFRDWLQGLQDAFDSDWETGKIMPNNYKNGSDDGVLAYKLLVQTGSRDKPIDISQLTKQRLVDADGIINPSAFYIYLTAWVSNDPVAYAASQANIRPHRPEWVHDKADYMPETRLRIPAAEPIEYAQFPFYLNGLRDTSDFVEAIEKVRTICSNYTSLGLSSYPNGYPFLFWEQYIGLRHWLLLFISVVLACTFLVCAVFLLNPWTAGIIVMVLALMTVELFGMMGLIGIKLSAVPVVILIASVGIGVEFTVHVALAFLTAIGDKNRRAVLALEHMFAPVLDGAVSTLLGVLMLAGSEFDFIVRYFFAVLAILTILGVLNGLVLLPVLLSFFGPYPEVSPANGLNRLPTPSPEPPPSVVRFAMPPGHTHSGSDSSDSEYSSQTTVSGLSEELRHYEAQQGAGGPAHQVIVEATENPVFAHSTVVHPESRHHPPSNPRQQPHLDSGSLPPGRQGQQPRRDPPREGLWPPPYRPRRDAFEISTEGHSGPSNRARWGPRGARSHNPRNPASTAMGSSVPGYCQPITTVTASASVTVAVHPPPVPGPGRNPRGGLCPGYPETDHGLFEDPHVPFHVRCERRDSKVEVIELQDVECEERPRGSSSN

Target ORF information:

RefSeq Version	XM_011518871.1
Organism	Homo sapiens(Human)
Definition	Homo sapiens patched 1 (PTCH1), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011518871.1

ORF Insert Sequence:

ATGTTTAATC CTCAACTCAT GATACAGACC CCTAAAGAAG AAGGTGCTAA TGTCCTGACC 
ACAGAAGCGC TCCTACAACA CCTGGACTCG GCACTCCAGG CCAGCCGTGT CCATGTATAC 
ATGTACAACA GGCAGTGGAA ATTGGAACAT TTGTGTTACA AATCAGGAGA GCTTATCACA 
GAAACAGGTT ACATGGATCA GATAATAGAA TATCTTTACC CTTGTTTGAT TATTACACCT 
TTGGACTGCT TCTGGGAAGG GGCGAAATTA CAGTCTGGGA CAGCATACCT CCTAGGTAAA 
CCTCCTTTGC GGTGGACAAA CTTCGACCCT TTGGAATTCC TGGAAGAGTT AAAGAAAATA 
AACTATCAAG TGGACAGCTG GGAGGAAATG CTGAATAAGG CTGAGGTTGG TCATGGTTAC 
ATGGACCGCC CCTGCCTCAA TCCGGCCGAT CCAGACTGCC CCGCCACAGC CCCCAACAAA 
AATTCAACCA AACCTCTTGA TATGGCCCTT GTTTTGAATG GTGGATGTCA TGGCTTATCC 
AGAAAGTATA TGCACTGGCA GGAGGAGTTG ATTGTGGGTG GCACAGTCAA GAACAGCACT 
GGAAAACTCG TCAGCGCCCA TGCCCTGCAG ACCATGTTCC AGTTAATGAC TCCCAAGCAA 
ATGTACGAGC ACTTCAAGGG GTACGAGTAT GTCTCACACA TCAACTGGAA CGAGGACAAA 
GCGGCAGCCA TCCTGGAGGC CTGGCAGAGG ACATATGTGG AGGTGGTTCA TCAGAGTGTC 
GCACAGAACT CCACTCAAAA GGTGCTTTCC TTCACCACCA CGACCCTGGA CGACATCCTG 
AAATCCTTCT CTGACGTCAG TGTCATCCGC GTGGCCAGCG GCTACTTACT CATGCTCGCC 
TATGCCTGTC TAACCATGCT GCGCTGGGAC TGCTCCAAGT CCCAGGGTGC CGTGGGGCTG 
GCTGGCGTCC TGCTGGTTGC ACTGTCAGTG GCTGCAGGAC TGGGCCTGTG CTCATTGATC 
GGAATTTCCT TTAACGCTGC AACAACTCAG GTTTTGCCAT TTCTCGCTCT TGGTGTTGGT 
GTGGATGATG TTTTTCTTCT GGCCCACGCC TTCAGTGAAA CAGGACAGAA TAAAAGAATC 
CCTTTTGAGG ACAGGACCGG GGAGTGCCTG AAGCGCACAG GAGCCAGCGT GGCCCTCACG 
TCCATCAGCA ATGTCACAGC CTTCTTCATG GCCGCGTTAA TCCCAATTCC CGCTCTGCGG 
GCGTTCTCCC TCCAGGCAGC GGTAGTAGTG GTGTTCAATT TTGCCATGGT TCTGCTCATT 
TTTCCTGCAA TTCTCAGCAT GGATTTATAT CGACGCGAGG ACAGGAGACT GGATATTTTC 
TGCTGTTTTA CAAGCCCCTG CGTCAGCAGA GTGATTCAGG TTGAACCTCA GGCCTACACC 
GACACACACG ACAATACCCG CTACAGCCCC CCACCTCCCT ACAGCAGCCA CAGCTTTGCC 
CATGAAACGC AGATTACCAT GCAGTCCACT GTCCAGCTCC GCACGGAGTA CGACCCCCAC 
ACGCACGTGT ACTACACCAC CGCTGAGCCG CGCTCCGAGA TCTCTGTGCA GCCCGTCACC 
GTGACACAGG ACACCCTCAG CTGCCAGAGC CCAGAGAGCA CCAGCTCCAC AAGGGACCTG 
CTCTCCCAGT TCTCCGACTC CAGCCTCCAC TGCCTCGAGC CCCCCTGTAC GAAGTGGACA 
CTCTCATCTT TTGCTGAGAA GCACTATGCT CCTTTCCTCT TGAAACCAAA AGCCAAGGTA 
GTGGTGATCT TCCTTTTTCT GGGCTTGCTG GGGGTCAGCC TTTATGGCAC CACCCGAGTG 
AGAGACGGGC TGGACCTTAC GGACATTGTA CCTCGGGAAA CCAGAGAATA TGACTTTATT 
GCTGCACAAT TCAAATACTT TTCTTTCTAC AACATGTATA TAGTCACCCA GAAAGCAGAC 
TACCCGAATA TCCAGCACTT ACTTTACGAC CTACACAGGA GTTTCAGTAA CGTGAAGTAT 
GTCATGTTGG AAGAAAACAA ACAGCTTCCC AAAATGTGGC TGCACTACTT CAGAGACTGG 
CTTCAGGGAC TTCAGGATGC ATTTGACAGT GACTGGGAAA CCGGGAAAAT CATGCCAAAC 
AATTACAAGA ATGGATCAGA CGATGGAGTC CTTGCCTACA AACTCCTGGT GCAAACCGGC 
AGCCGCGATA AGCCCATCGA CATCAGCCAG TTGACTAAAC AGCGTCTGGT GGATGCAGAT 
GGCATCATTA ATCCCAGCGC TTTCTACATC TACCTGACGG CTTGGGTCAG CAACGACCCC 
GTCGCGTATG CTGCCTCCCA GGCCAACATC CGGCCACACC GACCAGAATG GGTCCACGAC 
AAAGCCGACT ACATGCCTGA AACAAGGCTG AGAATCCCGG CAGCAGAGCC CATCGAGTAT 
GCCCAGTTCC CTTTCTACCT CAACGGCTTG CGGGACACCT CAGACTTTGT GGAGGCAATT 
GAAAAAGTAA GGACCATCTG CAGCAACTAT ACGAGCCTGG GGCTGTCCAG TTACCCCAAC 
GGCTACCCCT TCCTCTTCTG GGAGCAGTAC ATCGGCCTCC GCCACTGGCT GCTGCTGTTC 
ATCAGCGTGG TGTTGGCCTG CACATTCCTC GTGTGCGCTG TCTTCCTTCT GAACCCCTGG 
ACGGCCGGGA TCATTGTGAT GGTCCTGGCG CTGATGACGG TCGAGCTGTT CGGCATGATG 
GGCCTCATCG GAATCAAGCT CAGTGCCGTG CCCGTGGTCA TCCTGATCGC TTCTGTTGGC 
ATAGGAGTGG AGTTCACCGT TCACGTTGCT TTGGCCTTTC TGACGGCCAT CGGCGACAAG 
AACCGCAGGG CTGTGCTTGC CCTGGAGCAC ATGTTTGCAC CCGTCCTGGA TGGCGCCGTG 
TCCACTCTGC TGGGAGTGCT GATGCTGGCG GGATCTGAGT TCGACTTCAT TGTCAGGTAT 
TTCTTTGCTG TGCTGGCGAT CCTCACCATC CTCGGCGTTC TCAATGGGCT GGTTTTGCTT 
CCCGTGCTTT TGTCTTTCTT TGGACCATAT CCTGAGGTGT CTCCAGCCAA CGGCTTGAAC 
CGCCTGCCCA CACCCTCCCC TGAGCCACCC CCCAGCGTGG TCCGCTTCGC CATGCCGCCC 
GGCCACACGC ACAGCGGGTC TGATTCCTCC GACTCGGAGT ATAGTTCCCA GACGACAGTG 
TCAGGCCTCA GCGAGGAGCT TCGGCACTAC GAGGCCCAGC AGGGCGCGGG AGGCCCTGCC 
CACCAAGTGA TCGTGGAAGC CACAGAAAAC CCCGTCTTCG CCCACTCCAC TGTGGTCCAT 
CCCGAATCCA GGCATCACCC ACCCTCGAAC CCGAGACAGC AGCCCCACCT GGACTCAGGG 
TCCCTGCCTC CCGGACGGCA AGGCCAGCAG CCCCGCAGGG ACCCCCCCAG AGAAGGCTTG 
TGGCCACCCC CCTACAGACC GCGCAGAGAC GCTTTTGAAA TTTCTACTGA AGGGCATTCT 
GGCCCTAGCA ATAGGGCCCG CTGGGGCCCT CGCGGGGCCC GTTCTCACAA CCCTCGGAAC 
CCAGCGTCCA CTGCCATGGG CAGCTCCGTG CCCGGCTACT GCCAGCCCAT CACCACTGTG 
ACGGCTTCTG CCTCCGTGAC TGTCGCCGTG CACCCGCCGC CTGTCCCTGG GCCTGGGCGG 
AACCCCCGAG GGGGACTCTG CCCAGGCTAC CCTGAGACTG ACCACGGCCT GTTTGAGGAC 
CCCCACGTGC CTTTCCACGT CCGGTGTGAG AGGAGGGATT CGAAGGTGGA AGTCATTGAG 
CTGCAGGACG TGGAATGCGA GGAGAGGCCC CGGGGAAGCA GCTCCAACTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PTCH1 ( XM_011518869.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_011517171.1 Homo sapiens patched 1 (PTCH1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OHu17265
Clone ID Related Accession (Same CDS sequence)	XM_005252102.2 , XM_011518872.1 , NM_001083605.1 , NM_001083604.2 , NM_001083606.2 , NM_001083606.1 , XM_011518871.1 , XM_011518869.1 , NM_001083604.1 , XM_011518870.1 , NM_001083607.1 , NM_001083606.3 , NM_001083605.2 , NM_001083607.2
Accession Version	XM_011518869.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3891bp) Protein sequence SNP
Vector	pcDNA3.1⁺/C-(K)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-03-11
Organism	Homo sapiens(Human)
Product	protein patched homolog 1 isoform X1
Comment

ATGTTTAATC CTCAACTCAT GATACAGACC CCTAAAGAAG AAGGTGCTAA TGTCCTGACC 
ACAGAAGCGC TCCTACAACA CCTGGACTCG GCACTCCAGG CCAGCCGTGT CCATGTATAC 
ATGTACAACA GGCAGTGGAA ATTGGAACAT TTGTGTTACA AATCAGGAGA GCTTATCACA 
GAAACAGGTT ACATGGATCA GATAATAGAA TATCTTTACC CTTGTTTGAT TATTACACCT 
TTGGACTGCT TCTGGGAAGG GGCGAAATTA CAGTCTGGGA CAGCATACCT CCTAGGTAAA 
CCTCCTTTGC GGTGGACAAA CTTCGACCCT TTGGAATTCC TGGAAGAGTT AAAGAAAATA 
AACTATCAAG TGGACAGCTG GGAGGAAATG CTGAATAAGG CTGAGGTTGG TCATGGTTAC 
ATGGACCGCC CCTGCCTCAA TCCGGCCGAT CCAGACTGCC CCGCCACAGC CCCCAACAAA 
AATTCAACCA AACCTCTTGA TATGGCCCTT GTTTTGAATG GTGGATGTCA TGGCTTATCC 
AGAAAGTATA TGCACTGGCA GGAGGAGTTG ATTGTGGGTG GCACAGTCAA GAACAGCACT 
GGAAAACTCG TCAGCGCCCA TGCCCTGCAG ACCATGTTCC AGTTAATGAC TCCCAAGCAA 
ATGTACGAGC ACTTCAAGGG GTACGAGTAT GTCTCACACA TCAACTGGAA CGAGGACAAA 
GCGGCAGCCA TCCTGGAGGC CTGGCAGAGG ACATATGTGG AGGTGGTTCA TCAGAGTGTC 
GCACAGAACT CCACTCAAAA GGTGCTTTCC TTCACCACCA CGACCCTGGA CGACATCCTG 
AAATCCTTCT CTGACGTCAG TGTCATCCGC GTGGCCAGCG GCTACTTACT CATGCTCGCC 
TATGCCTGTC TAACCATGCT GCGCTGGGAC TGCTCCAAGT CCCAGGGTGC CGTGGGGCTG 
GCTGGCGTCC TGCTGGTTGC ACTGTCAGTG GCTGCAGGAC TGGGCCTGTG CTCATTGATC 
GGAATTTCCT TTAACGCTGC AACAACTCAG GTTTTGCCAT TTCTCGCTCT TGGTGTTGGT 
GTGGATGATG TTTTTCTTCT GGCCCACGCC TTCAGTGAAA CAGGACAGAA TAAAAGAATC 
CCTTTTGAGG ACAGGACCGG GGAGTGCCTG AAGCGCACAG GAGCCAGCGT GGCCCTCACG 
TCCATCAGCA ATGTCACAGC CTTCTTCATG GCCGCGTTAA TCCCAATTCC CGCTCTGCGG 
GCGTTCTCCC TCCAGGCAGC GGTAGTAGTG GTGTTCAATT TTGCCATGGT TCTGCTCATT 
TTTCCTGCAA TTCTCAGCAT GGATTTATAT CGACGCGAGG ACAGGAGACT GGATATTTTC 
TGCTGTTTTA CAAGCCCCTG CGTCAGCAGA GTGATTCAGG TTGAACCTCA GGCCTACACC 
GACACACACG ACAATACCCG CTACAGCCCC CCACCTCCCT ACAGCAGCCA CAGCTTTGCC 
CATGAAACGC AGATTACCAT GCAGTCCACT GTCCAGCTCC GCACGGAGTA CGACCCCCAC 
ACGCACGTGT ACTACACCAC CGCTGAGCCG CGCTCCGAGA TCTCTGTGCA GCCCGTCACC 
GTGACACAGG ACACCCTCAG CTGCCAGAGC CCAGAGAGCA CCAGCTCCAC AAGGGACCTG 
CTCTCCCAGT TCTCCGACTC CAGCCTCCAC TGCCTCGAGC CCCCCTGTAC GAAGTGGACA 
CTCTCATCTT TTGCTGAGAA GCACTATGCT CCTTTCCTCT TGAAACCAAA AGCCAAGGTA 
GTGGTGATCT TCCTTTTTCT GGGCTTGCTG GGGGTCAGCC TTTATGGCAC CACCCGAGTG 
AGAGACGGGC TGGACCTTAC GGACATTGTA CCTCGGGAAA CCAGAGAATA TGACTTTATT 
GCTGCACAAT TCAAATACTT TTCTTTCTAC AACATGTATA TAGTCACCCA GAAAGCAGAC 
TACCCGAATA TCCAGCACTT ACTTTACGAC CTACACAGGA GTTTCAGTAA CGTGAAGTAT 
GTCATGTTGG AAGAAAACAA ACAGCTTCCC AAAATGTGGC TGCACTACTT CAGAGACTGG 
CTTCAGGGAC TTCAGGATGC ATTTGACAGT GACTGGGAAA CCGGGAAAAT CATGCCAAAC 
AATTACAAGA ATGGATCAGA CGATGGAGTC CTTGCCTACA AACTCCTGGT GCAAACCGGC 
AGCCGCGATA AGCCCATCGA CATCAGCCAG TTGACTAAAC AGCGTCTGGT GGATGCAGAT 
GGCATCATTA ATCCCAGCGC TTTCTACATC TACCTGACGG CTTGGGTCAG CAACGACCCC 
GTCGCGTATG CTGCCTCCCA GGCCAACATC CGGCCACACC GACCAGAATG GGTCCACGAC 
AAAGCCGACT ACATGCCTGA AACAAGGCTG AGAATCCCGG CAGCAGAGCC CATCGAGTAT 
GCCCAGTTCC CTTTCTACCT CAACGGCTTG CGGGACACCT CAGACTTTGT GGAGGCAATT 
GAAAAAGTAA GGACCATCTG CAGCAACTAT ACGAGCCTGG GGCTGTCCAG TTACCCCAAC 
GGCTACCCCT TCCTCTTCTG GGAGCAGTAC ATCGGCCTCC GCCACTGGCT GCTGCTGTTC 
ATCAGCGTGG TGTTGGCCTG CACATTCCTC GTGTGCGCTG TCTTCCTTCT GAACCCCTGG 
ACGGCCGGGA TCATTGTGAT GGTCCTGGCG CTGATGACGG TCGAGCTGTT CGGCATGATG 
GGCCTCATCG GAATCAAGCT CAGTGCCGTG CCCGTGGTCA TCCTGATCGC TTCTGTTGGC 
ATAGGAGTGG AGTTCACCGT TCACGTTGCT TTGGCCTTTC TGACGGCCAT CGGCGACAAG 
AACCGCAGGG CTGTGCTTGC CCTGGAGCAC ATGTTTGCAC CCGTCCTGGA TGGCGCCGTG 
TCCACTCTGC TGGGAGTGCT GATGCTGGCG GGATCTGAGT TCGACTTCAT TGTCAGGTAT 
TTCTTTGCTG TGCTGGCGAT CCTCACCATC CTCGGCGTTC TCAATGGGCT GGTTTTGCTT 
CCCGTGCTTT TGTCTTTCTT TGGACCATAT CCTGAGGTGT CTCCAGCCAA CGGCTTGAAC 
CGCCTGCCCA CACCCTCCCC TGAGCCACCC CCCAGCGTGG TCCGCTTCGC CATGCCGCCC 
GGCCACACGC ACAGCGGGTC TGATTCCTCC GACTCGGAGT ATAGTTCCCA GACGACAGTG 
TCAGGCCTCA GCGAGGAGCT TCGGCACTAC GAGGCCCAGC AGGGCGCGGG AGGCCCTGCC 
CACCAAGTGA TCGTGGAAGC CACAGAAAAC CCCGTCTTCG CCCACTCCAC TGTGGTCCAT 
CCCGAATCCA GGCATCACCC ACCCTCGAAC CCGAGACAGC AGCCCCACCT GGACTCAGGG 
TCCCTGCCTC CCGGACGGCA AGGCCAGCAG CCCCGCAGGG ACCCCCCCAG AGAAGGCTTG 
TGGCCACCCC CCTACAGACC GCGCAGAGAC GCTTTTGAAA TTTCTACTGA AGGGCATTCT 
GGCCCTAGCA ATAGGGCCCG CTGGGGCCCT CGCGGGGCCC GTTCTCACAA CCCTCGGAAC 
CCAGCGTCCA CTGCCATGGG CAGCTCCGTG CCCGGCTACT GCCAGCCCAT CACCACTGTG 
ACGGCTTCTG CCTCCGTGAC TGTCGCCGTG CACCCGCCGC CTGTCCCTGG GCCTGGGCGG 
AACCCCCGAG GGGGACTCTG CCCAGGCTAC CCTGAGACTG ACCACGGCCT GTTTGAGGAC 
CCCCACGTGC CTTTCCACGT CCGGTGTGAG AGGAGGGATT CGAAGGTGGA AGTCATTGAG 
CTGCAGGACG TGGAATGCGA GGAGAGGCCC CGGGGAAGCA GCTCCAACTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq

XP_011517171.1

Translation

MFNPQLMIQTPKEEGANVLTTEALLQHLDSALQASRVHVYMYNRQWKLEHLCYKSGELITETGYMDQIIEYLYPCLIITPLDCFWEGAKLQSGTAYLLGKPPLRWTNFDPLEFLEELKKINYQVDSWEEMLNKAEVGHGYMDRPCLNPADPDCPATAPNKNSTKPLDMALVLNGGCHGLSRKYMHWQEELIVGGTVKNSTGKLVSAHALQTMFQLMTPKQMYEHFKGYEYVSHINWNEDKAAAILEAWQRTYVEVVHQSVAQNSTQKVLSFTTTTLDDILKSFSDVSVIRVASGYLLMLAYACLTMLRWDCSKSQGAVGLAGVLLVALSVAAGLGLCSLIGISFNAATTQVLPFLALGVGVDDVFLLAHAFSETGQNKRIPFEDRTGECLKRTGASVALTSISNVTAFFMAALIPIPALRAFSLQAAVVVVFNFAMVLLIFPAILSMDLYRREDRRLDIFCCFTSPCVSRVIQVEPQAYTDTHDNTRYSPPPPYSSHSFAHETQITMQSTVQLRTEYDPHTHVYYTTAEPRSEISVQPVTVTQDTLSCQSPESTSSTRDLLSQFSDSSLHCLEPPCTKWTLSSFAEKHYAPFLLKPKAKVVVIFLFLGLLGVSLYGTTRVRDGLDLTDIVPRETREYDFIAAQFKYFSFYNMYIVTQKADYPNIQHLLYDLHRSFSNVKYVMLEENKQLPKMWLHYFRDWLQGLQDAFDSDWETGKIMPNNYKNGSDDGVLAYKLLVQTGSRDKPIDISQLTKQRLVDADGIINPSAFYIYLTAWVSNDPVAYAASQANIRPHRPEWVHDKADYMPETRLRIPAAEPIEYAQFPFYLNGLRDTSDFVEAIEKVRTICSNYTSLGLSSYPNGYPFLFWEQYIGLRHWLLLFISVVLACTFLVCAVFLLNPWTAGIIVMVLALMTVELFGMMGLIGIKLSAVPVVILIASVGIGVEFTVHVALAFLTAIGDKNRRAVLALEHMFAPVLDGAVSTLLGVLMLAGSEFDFIVRYFFAVLAILTILGVLNGLVLLPVLLSFFGPYPEVSPANGLNRLPTPSPEPPPSVVRFAMPPGHTHSGSDSSDSEYSSQTTVSGLSEELRHYEAQQGAGGPAHQVIVEATENPVFAHSTVVHPESRHHPPSNPRQQPHLDSGSLPPGRQGQQPRRDPPREGLWPPPYRPRRDAFEISTEGHSGPSNRARWGPRGARSHNPRNPASTAMGSSVPGYCQPITTVTASASVTVAVHPPPVPGPGRNPRGGLCPGYPETDHGLFEDPHVPFHVRCERRDSKVEVIELQDVECEERPRGSSSN

Target ORF information:

RefSeq Version	XM_011518869.1
Organism	Homo sapiens(Human)
Definition	Homo sapiens patched 1 (PTCH1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011518869.1

ORF Insert Sequence:

ATGTTTAATC CTCAACTCAT GATACAGACC CCTAAAGAAG AAGGTGCTAA TGTCCTGACC 
ACAGAAGCGC TCCTACAACA CCTGGACTCG GCACTCCAGG CCAGCCGTGT CCATGTATAC 
ATGTACAACA GGCAGTGGAA ATTGGAACAT TTGTGTTACA AATCAGGAGA GCTTATCACA 
GAAACAGGTT ACATGGATCA GATAATAGAA TATCTTTACC CTTGTTTGAT TATTACACCT 
TTGGACTGCT TCTGGGAAGG GGCGAAATTA CAGTCTGGGA CAGCATACCT CCTAGGTAAA 
CCTCCTTTGC GGTGGACAAA CTTCGACCCT TTGGAATTCC TGGAAGAGTT AAAGAAAATA 
AACTATCAAG TGGACAGCTG GGAGGAAATG CTGAATAAGG CTGAGGTTGG TCATGGTTAC 
ATGGACCGCC CCTGCCTCAA TCCGGCCGAT CCAGACTGCC CCGCCACAGC CCCCAACAAA 
AATTCAACCA AACCTCTTGA TATGGCCCTT GTTTTGAATG GTGGATGTCA TGGCTTATCC 
AGAAAGTATA TGCACTGGCA GGAGGAGTTG ATTGTGGGTG GCACAGTCAA GAACAGCACT 
GGAAAACTCG TCAGCGCCCA TGCCCTGCAG ACCATGTTCC AGTTAATGAC TCCCAAGCAA 
ATGTACGAGC ACTTCAAGGG GTACGAGTAT GTCTCACACA TCAACTGGAA CGAGGACAAA 
GCGGCAGCCA TCCTGGAGGC CTGGCAGAGG ACATATGTGG AGGTGGTTCA TCAGAGTGTC 
GCACAGAACT CCACTCAAAA GGTGCTTTCC TTCACCACCA CGACCCTGGA CGACATCCTG 
AAATCCTTCT CTGACGTCAG TGTCATCCGC GTGGCCAGCG GCTACTTACT CATGCTCGCC 
TATGCCTGTC TAACCATGCT GCGCTGGGAC TGCTCCAAGT CCCAGGGTGC CGTGGGGCTG 
GCTGGCGTCC TGCTGGTTGC ACTGTCAGTG GCTGCAGGAC TGGGCCTGTG CTCATTGATC 
GGAATTTCCT TTAACGCTGC AACAACTCAG GTTTTGCCAT TTCTCGCTCT TGGTGTTGGT 
GTGGATGATG TTTTTCTTCT GGCCCACGCC TTCAGTGAAA CAGGACAGAA TAAAAGAATC 
CCTTTTGAGG ACAGGACCGG GGAGTGCCTG AAGCGCACAG GAGCCAGCGT GGCCCTCACG 
TCCATCAGCA ATGTCACAGC CTTCTTCATG GCCGCGTTAA TCCCAATTCC CGCTCTGCGG 
GCGTTCTCCC TCCAGGCAGC GGTAGTAGTG GTGTTCAATT TTGCCATGGT TCTGCTCATT 
TTTCCTGCAA TTCTCAGCAT GGATTTATAT CGACGCGAGG ACAGGAGACT GGATATTTTC 
TGCTGTTTTA CAAGCCCCTG CGTCAGCAGA GTGATTCAGG TTGAACCTCA GGCCTACACC 
GACACACACG ACAATACCCG CTACAGCCCC CCACCTCCCT ACAGCAGCCA CAGCTTTGCC 
CATGAAACGC AGATTACCAT GCAGTCCACT GTCCAGCTCC GCACGGAGTA CGACCCCCAC 
ACGCACGTGT ACTACACCAC CGCTGAGCCG CGCTCCGAGA TCTCTGTGCA GCCCGTCACC 
GTGACACAGG ACACCCTCAG CTGCCAGAGC CCAGAGAGCA CCAGCTCCAC AAGGGACCTG 
CTCTCCCAGT TCTCCGACTC CAGCCTCCAC TGCCTCGAGC CCCCCTGTAC GAAGTGGACA 
CTCTCATCTT TTGCTGAGAA GCACTATGCT CCTTTCCTCT TGAAACCAAA AGCCAAGGTA 
GTGGTGATCT TCCTTTTTCT GGGCTTGCTG GGGGTCAGCC TTTATGGCAC CACCCGAGTG 
AGAGACGGGC TGGACCTTAC GGACATTGTA CCTCGGGAAA CCAGAGAATA TGACTTTATT 
GCTGCACAAT TCAAATACTT TTCTTTCTAC AACATGTATA TAGTCACCCA GAAAGCAGAC 
TACCCGAATA TCCAGCACTT ACTTTACGAC CTACACAGGA GTTTCAGTAA CGTGAAGTAT 
GTCATGTTGG AAGAAAACAA ACAGCTTCCC AAAATGTGGC TGCACTACTT CAGAGACTGG 
CTTCAGGGAC TTCAGGATGC ATTTGACAGT GACTGGGAAA CCGGGAAAAT CATGCCAAAC 
AATTACAAGA ATGGATCAGA CGATGGAGTC CTTGCCTACA AACTCCTGGT GCAAACCGGC 
AGCCGCGATA AGCCCATCGA CATCAGCCAG TTGACTAAAC AGCGTCTGGT GGATGCAGAT 
GGCATCATTA ATCCCAGCGC TTTCTACATC TACCTGACGG CTTGGGTCAG CAACGACCCC 
GTCGCGTATG CTGCCTCCCA GGCCAACATC CGGCCACACC GACCAGAATG GGTCCACGAC 
AAAGCCGACT ACATGCCTGA AACAAGGCTG AGAATCCCGG CAGCAGAGCC CATCGAGTAT 
GCCCAGTTCC CTTTCTACCT CAACGGCTTG CGGGACACCT CAGACTTTGT GGAGGCAATT 
GAAAAAGTAA GGACCATCTG CAGCAACTAT ACGAGCCTGG GGCTGTCCAG TTACCCCAAC 
GGCTACCCCT TCCTCTTCTG GGAGCAGTAC ATCGGCCTCC GCCACTGGCT GCTGCTGTTC 
ATCAGCGTGG TGTTGGCCTG CACATTCCTC GTGTGCGCTG TCTTCCTTCT GAACCCCTGG 
ACGGCCGGGA TCATTGTGAT GGTCCTGGCG CTGATGACGG TCGAGCTGTT CGGCATGATG 
GGCCTCATCG GAATCAAGCT CAGTGCCGTG CCCGTGGTCA TCCTGATCGC TTCTGTTGGC 
ATAGGAGTGG AGTTCACCGT TCACGTTGCT TTGGCCTTTC TGACGGCCAT CGGCGACAAG 
AACCGCAGGG CTGTGCTTGC CCTGGAGCAC ATGTTTGCAC CCGTCCTGGA TGGCGCCGTG 
TCCACTCTGC TGGGAGTGCT GATGCTGGCG GGATCTGAGT TCGACTTCAT TGTCAGGTAT 
TTCTTTGCTG TGCTGGCGAT CCTCACCATC CTCGGCGTTC TCAATGGGCT GGTTTTGCTT 
CCCGTGCTTT TGTCTTTCTT TGGACCATAT CCTGAGGTGT CTCCAGCCAA CGGCTTGAAC 
CGCCTGCCCA CACCCTCCCC TGAGCCACCC CCCAGCGTGG TCCGCTTCGC CATGCCGCCC 
GGCCACACGC ACAGCGGGTC TGATTCCTCC GACTCGGAGT ATAGTTCCCA GACGACAGTG 
TCAGGCCTCA GCGAGGAGCT TCGGCACTAC GAGGCCCAGC AGGGCGCGGG AGGCCCTGCC 
CACCAAGTGA TCGTGGAAGC CACAGAAAAC CCCGTCTTCG CCCACTCCAC TGTGGTCCAT 
CCCGAATCCA GGCATCACCC ACCCTCGAAC CCGAGACAGC AGCCCCACCT GGACTCAGGG 
TCCCTGCCTC CCGGACGGCA AGGCCAGCAG CCCCGCAGGG ACCCCCCCAG AGAAGGCTTG 
TGGCCACCCC CCTACAGACC GCGCAGAGAC GCTTTTGAAA TTTCTACTGA AGGGCATTCT 
GGCCCTAGCA ATAGGGCCCG CTGGGGCCCT CGCGGGGCCC GTTCTCACAA CCCTCGGAAC 
CCAGCGTCCA CTGCCATGGG CAGCTCCGTG CCCGGCTACT GCCAGCCCAT CACCACTGTG 
ACGGCTTCTG CCTCCGTGAC TGTCGCCGTG CACCCGCCGC CTGTCCCTGG GCCTGGGCGG 
AACCCCCGAG GGGGACTCTG CCCAGGCTAC CCTGAGACTG ACCACGGCCT GTTTGAGGAC 
CCCCACGTGC CTTTCCACGT CCGGTGTGAG AGGAGGGATT CGAAGGTGGA AGTCATTGAG 
CTGCAGGACG TGGAATGCGA GGAGAGGCCC CGGGGAAGCA GCTCCAACTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PTCH1 ( XM_011518870.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_011517172.1 Homo sapiens patched 1 (PTCH1), transcript variant X3, mRNA.

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CloneID	OHu17265
Clone ID Related Accession (Same CDS sequence)	XM_005252102.2 , XM_011518872.1 , NM_001083605.1 , NM_001083604.2 , NM_001083606.2 , NM_001083606.1 , XM_011518871.1 , XM_011518869.1 , NM_001083604.1 , XM_011518870.1 , NM_001083607.1 , NM_001083606.3 , NM_001083605.2 , NM_001083607.2
Accession Version	XM_011518870.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3891bp) Protein sequence SNP
Vector	pcDNA3.1⁺/C-(K)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-03-11
Organism	Homo sapiens(Human)
Product	protein patched homolog 1 isoform X1
Comment

ATGTTTAATC CTCAACTCAT GATACAGACC CCTAAAGAAG AAGGTGCTAA TGTCCTGACC 
ACAGAAGCGC TCCTACAACA CCTGGACTCG GCACTCCAGG CCAGCCGTGT CCATGTATAC 
ATGTACAACA GGCAGTGGAA ATTGGAACAT TTGTGTTACA AATCAGGAGA GCTTATCACA 
GAAACAGGTT ACATGGATCA GATAATAGAA TATCTTTACC CTTGTTTGAT TATTACACCT 
TTGGACTGCT TCTGGGAAGG GGCGAAATTA CAGTCTGGGA CAGCATACCT CCTAGGTAAA 
CCTCCTTTGC GGTGGACAAA CTTCGACCCT TTGGAATTCC TGGAAGAGTT AAAGAAAATA 
AACTATCAAG TGGACAGCTG GGAGGAAATG CTGAATAAGG CTGAGGTTGG TCATGGTTAC 
ATGGACCGCC CCTGCCTCAA TCCGGCCGAT CCAGACTGCC CCGCCACAGC CCCCAACAAA 
AATTCAACCA AACCTCTTGA TATGGCCCTT GTTTTGAATG GTGGATGTCA TGGCTTATCC 
AGAAAGTATA TGCACTGGCA GGAGGAGTTG ATTGTGGGTG GCACAGTCAA GAACAGCACT 
GGAAAACTCG TCAGCGCCCA TGCCCTGCAG ACCATGTTCC AGTTAATGAC TCCCAAGCAA 
ATGTACGAGC ACTTCAAGGG GTACGAGTAT GTCTCACACA TCAACTGGAA CGAGGACAAA 
GCGGCAGCCA TCCTGGAGGC CTGGCAGAGG ACATATGTGG AGGTGGTTCA TCAGAGTGTC 
GCACAGAACT CCACTCAAAA GGTGCTTTCC TTCACCACCA CGACCCTGGA CGACATCCTG 
AAATCCTTCT CTGACGTCAG TGTCATCCGC GTGGCCAGCG GCTACTTACT CATGCTCGCC 
TATGCCTGTC TAACCATGCT GCGCTGGGAC TGCTCCAAGT CCCAGGGTGC CGTGGGGCTG 
GCTGGCGTCC TGCTGGTTGC ACTGTCAGTG GCTGCAGGAC TGGGCCTGTG CTCATTGATC 
GGAATTTCCT TTAACGCTGC AACAACTCAG GTTTTGCCAT TTCTCGCTCT TGGTGTTGGT 
GTGGATGATG TTTTTCTTCT GGCCCACGCC TTCAGTGAAA CAGGACAGAA TAAAAGAATC 
CCTTTTGAGG ACAGGACCGG GGAGTGCCTG AAGCGCACAG GAGCCAGCGT GGCCCTCACG 
TCCATCAGCA ATGTCACAGC CTTCTTCATG GCCGCGTTAA TCCCAATTCC CGCTCTGCGG 
GCGTTCTCCC TCCAGGCAGC GGTAGTAGTG GTGTTCAATT TTGCCATGGT TCTGCTCATT 
TTTCCTGCAA TTCTCAGCAT GGATTTATAT CGACGCGAGG ACAGGAGACT GGATATTTTC 
TGCTGTTTTA CAAGCCCCTG CGTCAGCAGA GTGATTCAGG TTGAACCTCA GGCCTACACC 
GACACACACG ACAATACCCG CTACAGCCCC CCACCTCCCT ACAGCAGCCA CAGCTTTGCC 
CATGAAACGC AGATTACCAT GCAGTCCACT GTCCAGCTCC GCACGGAGTA CGACCCCCAC 
ACGCACGTGT ACTACACCAC CGCTGAGCCG CGCTCCGAGA TCTCTGTGCA GCCCGTCACC 
GTGACACAGG ACACCCTCAG CTGCCAGAGC CCAGAGAGCA CCAGCTCCAC AAGGGACCTG 
CTCTCCCAGT TCTCCGACTC CAGCCTCCAC TGCCTCGAGC CCCCCTGTAC GAAGTGGACA 
CTCTCATCTT TTGCTGAGAA GCACTATGCT CCTTTCCTCT TGAAACCAAA AGCCAAGGTA 
GTGGTGATCT TCCTTTTTCT GGGCTTGCTG GGGGTCAGCC TTTATGGCAC CACCCGAGTG 
AGAGACGGGC TGGACCTTAC GGACATTGTA CCTCGGGAAA CCAGAGAATA TGACTTTATT 
GCTGCACAAT TCAAATACTT TTCTTTCTAC AACATGTATA TAGTCACCCA GAAAGCAGAC 
TACCCGAATA TCCAGCACTT ACTTTACGAC CTACACAGGA GTTTCAGTAA CGTGAAGTAT 
GTCATGTTGG AAGAAAACAA ACAGCTTCCC AAAATGTGGC TGCACTACTT CAGAGACTGG 
CTTCAGGGAC TTCAGGATGC ATTTGACAGT GACTGGGAAA CCGGGAAAAT CATGCCAAAC 
AATTACAAGA ATGGATCAGA CGATGGAGTC CTTGCCTACA AACTCCTGGT GCAAACCGGC 
AGCCGCGATA AGCCCATCGA CATCAGCCAG TTGACTAAAC AGCGTCTGGT GGATGCAGAT 
GGCATCATTA ATCCCAGCGC TTTCTACATC TACCTGACGG CTTGGGTCAG CAACGACCCC 
GTCGCGTATG CTGCCTCCCA GGCCAACATC CGGCCACACC GACCAGAATG GGTCCACGAC 
AAAGCCGACT ACATGCCTGA AACAAGGCTG AGAATCCCGG CAGCAGAGCC CATCGAGTAT 
GCCCAGTTCC CTTTCTACCT CAACGGCTTG CGGGACACCT CAGACTTTGT GGAGGCAATT 
GAAAAAGTAA GGACCATCTG CAGCAACTAT ACGAGCCTGG GGCTGTCCAG TTACCCCAAC 
GGCTACCCCT TCCTCTTCTG GGAGCAGTAC ATCGGCCTCC GCCACTGGCT GCTGCTGTTC 
ATCAGCGTGG TGTTGGCCTG CACATTCCTC GTGTGCGCTG TCTTCCTTCT GAACCCCTGG 
ACGGCCGGGA TCATTGTGAT GGTCCTGGCG CTGATGACGG TCGAGCTGTT CGGCATGATG 
GGCCTCATCG GAATCAAGCT CAGTGCCGTG CCCGTGGTCA TCCTGATCGC TTCTGTTGGC 
ATAGGAGTGG AGTTCACCGT TCACGTTGCT TTGGCCTTTC TGACGGCCAT CGGCGACAAG 
AACCGCAGGG CTGTGCTTGC CCTGGAGCAC ATGTTTGCAC CCGTCCTGGA TGGCGCCGTG 
TCCACTCTGC TGGGAGTGCT GATGCTGGCG GGATCTGAGT TCGACTTCAT TGTCAGGTAT 
TTCTTTGCTG TGCTGGCGAT CCTCACCATC CTCGGCGTTC TCAATGGGCT GGTTTTGCTT 
CCCGTGCTTT TGTCTTTCTT TGGACCATAT CCTGAGGTGT CTCCAGCCAA CGGCTTGAAC 
CGCCTGCCCA CACCCTCCCC TGAGCCACCC CCCAGCGTGG TCCGCTTCGC CATGCCGCCC 
GGCCACACGC ACAGCGGGTC TGATTCCTCC GACTCGGAGT ATAGTTCCCA GACGACAGTG 
TCAGGCCTCA GCGAGGAGCT TCGGCACTAC GAGGCCCAGC AGGGCGCGGG AGGCCCTGCC 
CACCAAGTGA TCGTGGAAGC CACAGAAAAC CCCGTCTTCG CCCACTCCAC TGTGGTCCAT 
CCCGAATCCA GGCATCACCC ACCCTCGAAC CCGAGACAGC AGCCCCACCT GGACTCAGGG 
TCCCTGCCTC CCGGACGGCA AGGCCAGCAG CCCCGCAGGG ACCCCCCCAG AGAAGGCTTG 
TGGCCACCCC CCTACAGACC GCGCAGAGAC GCTTTTGAAA TTTCTACTGA AGGGCATTCT 
GGCCCTAGCA ATAGGGCCCG CTGGGGCCCT CGCGGGGCCC GTTCTCACAA CCCTCGGAAC 
CCAGCGTCCA CTGCCATGGG CAGCTCCGTG CCCGGCTACT GCCAGCCCAT CACCACTGTG 
ACGGCTTCTG CCTCCGTGAC TGTCGCCGTG CACCCGCCGC CTGTCCCTGG GCCTGGGCGG 
AACCCCCGAG GGGGACTCTG CCCAGGCTAC CCTGAGACTG ACCACGGCCT GTTTGAGGAC 
CCCCACGTGC CTTTCCACGT CCGGTGTGAG AGGAGGGATT CGAAGGTGGA AGTCATTGAG 
CTGCAGGACG TGGAATGCGA GGAGAGGCCC CGGGGAAGCA GCTCCAACTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq

XP_011517172.1

Translation

MFNPQLMIQTPKEEGANVLTTEALLQHLDSALQASRVHVYMYNRQWKLEHLCYKSGELITETGYMDQIIEYLYPCLIITPLDCFWEGAKLQSGTAYLLGKPPLRWTNFDPLEFLEELKKINYQVDSWEEMLNKAEVGHGYMDRPCLNPADPDCPATAPNKNSTKPLDMALVLNGGCHGLSRKYMHWQEELIVGGTVKNSTGKLVSAHALQTMFQLMTPKQMYEHFKGYEYVSHINWNEDKAAAILEAWQRTYVEVVHQSVAQNSTQKVLSFTTTTLDDILKSFSDVSVIRVASGYLLMLAYACLTMLRWDCSKSQGAVGLAGVLLVALSVAAGLGLCSLIGISFNAATTQVLPFLALGVGVDDVFLLAHAFSETGQNKRIPFEDRTGECLKRTGASVALTSISNVTAFFMAALIPIPALRAFSLQAAVVVVFNFAMVLLIFPAILSMDLYRREDRRLDIFCCFTSPCVSRVIQVEPQAYTDTHDNTRYSPPPPYSSHSFAHETQITMQSTVQLRTEYDPHTHVYYTTAEPRSEISVQPVTVTQDTLSCQSPESTSSTRDLLSQFSDSSLHCLEPPCTKWTLSSFAEKHYAPFLLKPKAKVVVIFLFLGLLGVSLYGTTRVRDGLDLTDIVPRETREYDFIAAQFKYFSFYNMYIVTQKADYPNIQHLLYDLHRSFSNVKYVMLEENKQLPKMWLHYFRDWLQGLQDAFDSDWETGKIMPNNYKNGSDDGVLAYKLLVQTGSRDKPIDISQLTKQRLVDADGIINPSAFYIYLTAWVSNDPVAYAASQANIRPHRPEWVHDKADYMPETRLRIPAAEPIEYAQFPFYLNGLRDTSDFVEAIEKVRTICSNYTSLGLSSYPNGYPFLFWEQYIGLRHWLLLFISVVLACTFLVCAVFLLNPWTAGIIVMVLALMTVELFGMMGLIGIKLSAVPVVILIASVGIGVEFTVHVALAFLTAIGDKNRRAVLALEHMFAPVLDGAVSTLLGVLMLAGSEFDFIVRYFFAVLAILTILGVLNGLVLLPVLLSFFGPYPEVSPANGLNRLPTPSPEPPPSVVRFAMPPGHTHSGSDSSDSEYSSQTTVSGLSEELRHYEAQQGAGGPAHQVIVEATENPVFAHSTVVHPESRHHPPSNPRQQPHLDSGSLPPGRQGQQPRRDPPREGLWPPPYRPRRDAFEISTEGHSGPSNRARWGPRGARSHNPRNPASTAMGSSVPGYCQPITTVTASASVTVAVHPPPVPGPGRNPRGGLCPGYPETDHGLFEDPHVPFHVRCERRDSKVEVIELQDVECEERPRGSSSN

Target ORF information:

RefSeq Version	XM_011518870.1
Organism	Homo sapiens(Human)
Definition	Homo sapiens patched 1 (PTCH1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011518870.1

ORF Insert Sequence:

ATGTTTAATC CTCAACTCAT GATACAGACC CCTAAAGAAG AAGGTGCTAA TGTCCTGACC 
ACAGAAGCGC TCCTACAACA CCTGGACTCG GCACTCCAGG CCAGCCGTGT CCATGTATAC 
ATGTACAACA GGCAGTGGAA ATTGGAACAT TTGTGTTACA AATCAGGAGA GCTTATCACA 
GAAACAGGTT ACATGGATCA GATAATAGAA TATCTTTACC CTTGTTTGAT TATTACACCT 
TTGGACTGCT TCTGGGAAGG GGCGAAATTA CAGTCTGGGA CAGCATACCT CCTAGGTAAA 
CCTCCTTTGC GGTGGACAAA CTTCGACCCT TTGGAATTCC TGGAAGAGTT AAAGAAAATA 
AACTATCAAG TGGACAGCTG GGAGGAAATG CTGAATAAGG CTGAGGTTGG TCATGGTTAC 
ATGGACCGCC CCTGCCTCAA TCCGGCCGAT CCAGACTGCC CCGCCACAGC CCCCAACAAA 
AATTCAACCA AACCTCTTGA TATGGCCCTT GTTTTGAATG GTGGATGTCA TGGCTTATCC 
AGAAAGTATA TGCACTGGCA GGAGGAGTTG ATTGTGGGTG GCACAGTCAA GAACAGCACT 
GGAAAACTCG TCAGCGCCCA TGCCCTGCAG ACCATGTTCC AGTTAATGAC TCCCAAGCAA 
ATGTACGAGC ACTTCAAGGG GTACGAGTAT GTCTCACACA TCAACTGGAA CGAGGACAAA 
GCGGCAGCCA TCCTGGAGGC CTGGCAGAGG ACATATGTGG AGGTGGTTCA TCAGAGTGTC 
GCACAGAACT CCACTCAAAA GGTGCTTTCC TTCACCACCA CGACCCTGGA CGACATCCTG 
AAATCCTTCT CTGACGTCAG TGTCATCCGC GTGGCCAGCG GCTACTTACT CATGCTCGCC 
TATGCCTGTC TAACCATGCT GCGCTGGGAC TGCTCCAAGT CCCAGGGTGC CGTGGGGCTG 
GCTGGCGTCC TGCTGGTTGC ACTGTCAGTG GCTGCAGGAC TGGGCCTGTG CTCATTGATC 
GGAATTTCCT TTAACGCTGC AACAACTCAG GTTTTGCCAT TTCTCGCTCT TGGTGTTGGT 
GTGGATGATG TTTTTCTTCT GGCCCACGCC TTCAGTGAAA CAGGACAGAA TAAAAGAATC 
CCTTTTGAGG ACAGGACCGG GGAGTGCCTG AAGCGCACAG GAGCCAGCGT GGCCCTCACG 
TCCATCAGCA ATGTCACAGC CTTCTTCATG GCCGCGTTAA TCCCAATTCC CGCTCTGCGG 
GCGTTCTCCC TCCAGGCAGC GGTAGTAGTG GTGTTCAATT TTGCCATGGT TCTGCTCATT 
TTTCCTGCAA TTCTCAGCAT GGATTTATAT CGACGCGAGG ACAGGAGACT GGATATTTTC 
TGCTGTTTTA CAAGCCCCTG CGTCAGCAGA GTGATTCAGG TTGAACCTCA GGCCTACACC 
GACACACACG ACAATACCCG CTACAGCCCC CCACCTCCCT ACAGCAGCCA CAGCTTTGCC 
CATGAAACGC AGATTACCAT GCAGTCCACT GTCCAGCTCC GCACGGAGTA CGACCCCCAC 
ACGCACGTGT ACTACACCAC CGCTGAGCCG CGCTCCGAGA TCTCTGTGCA GCCCGTCACC 
GTGACACAGG ACACCCTCAG CTGCCAGAGC CCAGAGAGCA CCAGCTCCAC AAGGGACCTG 
CTCTCCCAGT TCTCCGACTC CAGCCTCCAC TGCCTCGAGC CCCCCTGTAC GAAGTGGACA 
CTCTCATCTT TTGCTGAGAA GCACTATGCT CCTTTCCTCT TGAAACCAAA AGCCAAGGTA 
GTGGTGATCT TCCTTTTTCT GGGCTTGCTG GGGGTCAGCC TTTATGGCAC CACCCGAGTG 
AGAGACGGGC TGGACCTTAC GGACATTGTA CCTCGGGAAA CCAGAGAATA TGACTTTATT 
GCTGCACAAT TCAAATACTT TTCTTTCTAC AACATGTATA TAGTCACCCA GAAAGCAGAC 
TACCCGAATA TCCAGCACTT ACTTTACGAC CTACACAGGA GTTTCAGTAA CGTGAAGTAT 
GTCATGTTGG AAGAAAACAA ACAGCTTCCC AAAATGTGGC TGCACTACTT CAGAGACTGG 
CTTCAGGGAC TTCAGGATGC ATTTGACAGT GACTGGGAAA CCGGGAAAAT CATGCCAAAC 
AATTACAAGA ATGGATCAGA CGATGGAGTC CTTGCCTACA AACTCCTGGT GCAAACCGGC 
AGCCGCGATA AGCCCATCGA CATCAGCCAG TTGACTAAAC AGCGTCTGGT GGATGCAGAT 
GGCATCATTA ATCCCAGCGC TTTCTACATC TACCTGACGG CTTGGGTCAG CAACGACCCC 
GTCGCGTATG CTGCCTCCCA GGCCAACATC CGGCCACACC GACCAGAATG GGTCCACGAC 
AAAGCCGACT ACATGCCTGA AACAAGGCTG AGAATCCCGG CAGCAGAGCC CATCGAGTAT 
GCCCAGTTCC CTTTCTACCT CAACGGCTTG CGGGACACCT CAGACTTTGT GGAGGCAATT 
GAAAAAGTAA GGACCATCTG CAGCAACTAT ACGAGCCTGG GGCTGTCCAG TTACCCCAAC 
GGCTACCCCT TCCTCTTCTG GGAGCAGTAC ATCGGCCTCC GCCACTGGCT GCTGCTGTTC 
ATCAGCGTGG TGTTGGCCTG CACATTCCTC GTGTGCGCTG TCTTCCTTCT GAACCCCTGG 
ACGGCCGGGA TCATTGTGAT GGTCCTGGCG CTGATGACGG TCGAGCTGTT CGGCATGATG 
GGCCTCATCG GAATCAAGCT CAGTGCCGTG CCCGTGGTCA TCCTGATCGC TTCTGTTGGC 
ATAGGAGTGG AGTTCACCGT TCACGTTGCT TTGGCCTTTC TGACGGCCAT CGGCGACAAG 
AACCGCAGGG CTGTGCTTGC CCTGGAGCAC ATGTTTGCAC CCGTCCTGGA TGGCGCCGTG 
TCCACTCTGC TGGGAGTGCT GATGCTGGCG GGATCTGAGT TCGACTTCAT TGTCAGGTAT 
TTCTTTGCTG TGCTGGCGAT CCTCACCATC CTCGGCGTTC TCAATGGGCT GGTTTTGCTT 
CCCGTGCTTT TGTCTTTCTT TGGACCATAT CCTGAGGTGT CTCCAGCCAA CGGCTTGAAC 
CGCCTGCCCA CACCCTCCCC TGAGCCACCC CCCAGCGTGG TCCGCTTCGC CATGCCGCCC 
GGCCACACGC ACAGCGGGTC TGATTCCTCC GACTCGGAGT ATAGTTCCCA GACGACAGTG 
TCAGGCCTCA GCGAGGAGCT TCGGCACTAC GAGGCCCAGC AGGGCGCGGG AGGCCCTGCC 
CACCAAGTGA TCGTGGAAGC CACAGAAAAC CCCGTCTTCG CCCACTCCAC TGTGGTCCAT 
CCCGAATCCA GGCATCACCC ACCCTCGAAC CCGAGACAGC AGCCCCACCT GGACTCAGGG 
TCCCTGCCTC CCGGACGGCA AGGCCAGCAG CCCCGCAGGG ACCCCCCCAG AGAAGGCTTG 
TGGCCACCCC CCTACAGACC GCGCAGAGAC GCTTTTGAAA TTTCTACTGA AGGGCATTCT 
GGCCCTAGCA ATAGGGCCCG CTGGGGCCCT CGCGGGGCCC GTTCTCACAA CCCTCGGAAC 
CCAGCGTCCA CTGCCATGGG CAGCTCCGTG CCCGGCTACT GCCAGCCCAT CACCACTGTG 
ACGGCTTCTG CCTCCGTGAC TGTCGCCGTG CACCCGCCGC CTGTCCCTGG GCCTGGGCGG 
AACCCCCGAG GGGGACTCTG CCCAGGCTAC CCTGAGACTG ACCACGGCCT GTTTGAGGAC 
CCCCACGTGC CTTTCCACGT CCGGTGTGAG AGGAGGGATT CGAAGGTGGA AGTCATTGAG 
CTGCAGGACG TGGAATGCGA GGAGAGGCCC CGGGGAAGCA GCTCCAACTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PTCH1 ( NM_001354919.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001341848.1 Homo sapiens patched 1 (PTCH1), transcript variant 9, mRNA.

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CloneID	OHu102968
Clone ID Related Accession (Same CDS sequence)	NM_001354919.1
Accession Version	NM_001354919.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 552bp) Protein sequence SNP
Vector	pcDNA3.1⁺/C-(K)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-07-20
Organism	Homo sapiens(human)
Product	protein patched homolog 1 isoform 9
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BC043542.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC043542.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGGGAAGG CTACTGGCCG GAAAGCGCCG CTGTGGCTGA GAGCGAAGTT TCAGAGACTC 
TTATTTAAAC TGGGTTGTTA CATTCAAAAA AACTGCGGCA AGTTCTTGGT TGTGGGCCTC 
CTCATATTTG GGGCCTTCGC GGTGGGATTA AAAGCAGCGA ACCTCGAGAC CAACGTGGAG 
GAGCTGTGGG TGGAAGTTGG AGGACGAGTA AGTCGTGAAT TAAATTATAC TCGCCAGAAG 
ATTGGAGAAG AGGCTATGTT TAATCCTCAA CTCATGATAC AGACCCCTAA AGAAGAAGGT 
GCTAATGTCC TGACCACAGA AGCGCTCCTA CAACACCTGG ACTCGGCACT CCAGGCCAGC 
CGTGTCCATG TATACATGTA CAACAGGCAG TGGAAATTGG AACATTTGTG TTACAAATCA 
GGAGAGCTTA TCACAGAAAC AGGTTACATG GATCAGATAA TAGAATATCT TTACCCTTGT 
TTGATTATTA CACCTTTGGA CTGCTTCTGG GAAGGGGCGA AATTACAGTC TGGGACAGCA 
TACCTCCTGT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001341848.1
CDS	587..1138
Translation	MGKATGRKAPLWLRAKFQRLLFKLGCYIQKNCGKFLVVGLLIFGAFAVGLKAANLETNVEELWVEVGGRVSRELNYTRQKIGEEAMFNPQLMIQTPKEEGANVLTTEALLQHLDSALQASRVHVYMYNRQWKLEHLCYKSGELITETGYMDQIIEYLYPCLIITPLDCFWEGAKLQSGTAYLL

Target ORF information:

RefSeq Version	NM_001354919.1
Organism	Homo sapiens(human)
Definition	Homo sapiens patched 1 (PTCH1), transcript variant 9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001354919.1

ORF Insert Sequence:

ATGGGGAAGG CTACTGGCCG GAAAGCGCCG CTGTGGCTGA GAGCGAAGTT TCAGAGACTC 
TTATTTAAAC TGGGTTGTTA CATTCAAAAA AACTGCGGCA AGTTCTTGGT TGTGGGCCTC 
CTCATATTTG GGGCCTTCGC GGTGGGATTA AAAGCAGCGA ACCTCGAGAC CAACGTGGAG 
GAGCTGTGGG TGGAAGTTGG AGGACGAGTA AGTCGTGAAT TAAATTATAC TCGCCAGAAG 
ATTGGAGAAG AGGCTATGTT TAATCCTCAA CTCATGATAC AGACCCCTAA AGAAGAAGGT 
GCTAATGTCC TGACCACAGA AGCGCTCCTA CAACACCTGG ACTCGGCACT CCAGGCCAGC 
CGTGTCCATG TATACATGTA CAACAGGCAG TGGAAATTGG AACATTTGTG TTACAAATCA 
GGAGAGCTTA TCACAGAAAC AGGTTACATG GATCAGATAA TAGAATATCT TTACCCTTGT 
TTGATTATTA CACCTTTGGA CTGCTTCTGG GAAGGGGCGA AATTACAGTC TGGGACAGCA 
TACCTCCTGT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PTCH1 ( NM_001354918.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001341847.1 Homo sapiens patched 1 (PTCH1), transcript variant 8, mRNA.

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CloneID	OHu59996
Clone ID Related Accession (Same CDS sequence)	NM_001354918.1 , NM_001354918.2
Accession Version	NM_001354918.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4188bp) Protein sequence SNP
Vector	pcDNA3.1⁺/C-(K)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2020-01-04
Organism	Homo sapiens(human)
Product	protein patched homolog 1 isoform 8
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL161729.27. On Jul 26, 2019 this sequence version replaced NM_001354918.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## CDS exon combination :: KY652975.1 [ECO:0000331] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCCTCGG CTGGTAACGC CGCCGAGCCC CAGGACCGCG GCGGCGGCGG CAGCGGCTGT 
ATCGGTGCCC CGGGACGGCC GGCTGGAGGC GGGAGGCGCA GACGGACGGG GGGGCTGCGC 
CGTGCTGCCG CGCCGGACCG GGACTATCTG CACCGGCCCA GCTACTGCGA CGCCGCCTTC 
GCTCTGGAGC AGATTTCCAA GGGGAAGGCT ACTGGCCGGA AAGCGCCGCT GTGGCTGAGA 
GCGAAGTTTC AGAGACTCTT ATTTAAACTG GGTTGTTACA TTCAAAAAAA CTGCGGCAAG 
TTCTTGGTTG TGGGCCTCCT CATATTTGGG GCCTTCGCGG TGGGATTAAA AGCAGCGAAC 
CTCGAGACCA ACGTGGAGGA GCTGTGGGTG GAAGTTGGAG GACGAGTAAG TCGTGAATTA 
AATTATACTC GCCAGAAGAT TGGAGAAGAG GCTATGTTTA ATCCTCAACT CATGATACAG 
ACCCCTAAAG AAGAAGGTGC TAATGTCCTG ACCACAGAAG CGCTCCTACA ACACCTGGAC 
TCGGCACTCC AGGCCAGCCG TGTCCATGTA TACATGTACA ACAGGCAGTG GAAATTGGAA 
CATTTGTGTT ACAAATCAGG AGAGCTTATC ACAGAAACAG GTTACATGGA TCAGATAATA 
GAATATCTTT ACCCTTGTTT GATTATTACA CCTTTGGACT GCTTCTGGGA AGGGGCGAAA 
TTACAGTCTG GGACAGCATA CCTCCTAGGT AAACCTCCTT TGCGGTGGAC AAACTTCGAC 
CCTTTGGAAT TCCTGGAAGA GTTAAAGAAA ATAAACTATC AAGTGGACAG CTGGGAGGAA 
ATGCTGAATA AGGCTGAGGT TGGTCATGGT TACATGGACC GCCCCTGCCT CAATCCGGCC 
GATCCAGACT GCCCCGCCAC AGCCCCCAAC AAAAATTCAA CCAAACCTCT TGATATGGCC 
CTTGTTTTGA ATGGTGGATG TCATGGCTTA TCCAGAAAGT ATATGCACTG GCAGGAGGAG 
TTGATTGTGG GTGGCACAGT CAAGAACAGC ACTGGAAAAC TCGTCAGCGC CCATGCCCTG 
CAGACCATGT TCCAGTTAAT GACTCCCAAG CAAATGTACG AGCACTTCAA GGGGTACGAG 
TATGTCTCAC ACATCAACTG GAACGAGGAC AAAGCGGCAG CCATCCTGGA GGCCTGGCAG 
AGGACATATG TGGAGGTGGT TCATCAGAGT GTCGCACAGA ACTCCACTCA AAAGGTGCTT 
TCCTTCACCA CCACGACCCT GGACGACATC CTGAAATCCT TCTCTGACGT CAGTGTCATC 
CGCGTGGCCA GCGGCTACTT ACTCATGGTT TTGCCATTTC TCGCTCTTGG TGTTGGTGTG 
GATGATGTTT TTCTTCTGGC CCACGCCTTC AGTGAAACAG GACAGAATAA AAGAATCCCT 
TTTGAGGACA GGACCGGGGA GTGCCTGAAG CGCACAGGAG CCAGCGTGGC CCTCACGTCC 
ATCAGCAATG TCACAGCCTT CTTCATGGCC GCGTTAATCC CAATTCCCGC TCTGCGGGCG 
TTCTCCCTCC AGGCAGCGGT AGTAGTGGTG TTCAATTTTG CCATGGTTCT GCTCATTTTT 
CCTGCAATTC TCAGCATGGA TTTATATCGA CGCGAGGACA GGAGACTGGA TATTTTCTGC 
TGTTTTACAA GCCCCTGCGT CAGCAGAGTG ATTCAGGTTG AACCTCAGGC CTACACCGAC 
ACACACGACA ATACCCGCTA CAGCCCCCCA CCTCCCTACA GCAGCCACAG CTTTGCCCAT 
GAAACGCAGA TTACCATGCA GTCCACTGTC CAGCTCCGCA CGGAGTACGA CCCCCACACG 
CACGTGTACT ACACCACCGC TGAGCCGCGC TCCGAGATCT CTGTGCAGCC CGTCACCGTG 
ACACAGGACA CCCTCAGCTG CCAGAGCCCA GAGAGCACCA GCTCCACAAG GGACCTGCTC 
TCCCAGTTCT CCGACTCCAG CCTCCACTGC CTCGAGCCCC CCTGTACGAA GTGGACACTC 
TCATCTTTTG CTGAGAAGCA CTATGCTCCT TTCCTCTTGA AACCAAAAGC CAAGGTAGTG 
GTGATCTTCC TTTTTCTGGG CTTGCTGGGG GTCAGCCTTT ATGGCACCAC CCGAGTGAGA 
GACGGGCTGG ACCTTACGGA CATTGTACCT CGGGAAACCA GAGAATATGA CTTTATTGCT 
GCACAATTCA AATACTTTTC TTTCTACAAC ATGTATATAG TCACCCAGAA AGCAGACTAC 
CCGAATATCC AGCACTTACT TTACGACCTA CACAGGAGTT TCAGTAACGT GAAGTATGTC 
ATGTTGGAAG AAAACAAACA GCTTCCCAAA ATGTGGCTGC ACTACTTCAG AGACTGGCTT 
CAGGGACTTC AGGATGCATT TGACAGTGAC TGGGAAACCG GGAAAATCAT GCCAAACAAT 
TACAAGAATG GATCAGACGA TGGAGTCCTT GCCTACAAAC TCCTGGTGCA AACCGGCAGC 
CGCGATAAGC CCATCGACAT CAGCCAGTTG ACTAAACAGC GTCTGGTGGA TGCAGATGGC 
ATCATTAATC CCAGCGCTTT CTACATCTAC CTGACGGCTT GGGTCAGCAA CGACCCCGTC 
GCGTATGCTG CCTCCCAGGC CAACATCCGG CCACACCGAC CAGAATGGGT CCACGACAAA 
GCCGACTACA TGCCTGAAAC AAGGCTGAGA ATCCCGGCAG CAGAGCCCAT CGAGTATGCC 
CAGTTCCCTT TCTACCTCAA CGGCTTGCGG GACACCTCAG ACTTTGTGGA GGCAATTGAA 
AAAGTAAGGA CCATCTGCAG CAACTATACG AGCCTGGGGC TGTCCAGTTA CCCCAACGGC 
TACCCCTTCC TCTTCTGGGA GCAGTACATC GGCCTCCGCC ACTGGCTGCT GCTGTTCATC 
AGCGTGGTGT TGGCCTGCAC ATTCCTCGTG TGCGCTGTCT TCCTTCTGAA CCCCTGGACG 
GCCGGGATCA TTGTGATGGT CCTGGCGCTG ATGACGGTCG AGCTGTTCGG CATGATGGGC 
CTCATCGGAA TCAAGCTCAG TGCCGTGCCC GTGGTCATCC TGATCGCTTC TGTTGGCATA 
GGAGTGGAGT TCACCGTTCA CGTTGCTTTG GCCTTTCTGA CGGCCATCGG CGACAAGAAC 
CGCAGGGCTG TGCTTGCCCT GGAGCACATG TTTGCACCCG TCCTGGATGG CGCCGTGTCC 
ACTCTGCTGG GAGTGCTGAT GCTGGCGGGA TCTGAGTTCG ACTTCATTGT CAGGTATTTC 
TTTGCTGTGC TGGCGATCCT CACCATCCTC GGCGTTCTCA ATGGGCTGGT TTTGCTTCCC 
GTGCTTTTGT CTTTCTTTGG ACCATATCCT GAGGTGTCTC CAGCCAACGG CTTGAACCGC 
CTGCCCACAC CCTCCCCTGA GCCACCCCCC AGCGTGGTCC GCTTCGCCAT GCCGCCCGGC 
CACACGCACA GCGGGTCTGA TTCCTCCGAC TCGGAGTATA GTTCCCAGAC GACAGTGTCA 
GGCCTCAGCG AGGAGCTTCG GCACTACGAG GCCCAGCAGG GCGCGGGAGG CCCTGCCCAC 
CAAGTGATCG TGGAAGCCAC AGAAAACCCC GTCTTCGCCC ACTCCACTGT GGTCCATCCC 
GAATCCAGGC ATCACCCACC CTCGAACCCG AGACAGCAGC CCCACCTGGA CTCAGGGTCC 
CTGCCTCCCG GACGGCAAGG CCAGCAGCCC CGCAGGGACC CCCCCAGAGA AGGCTTGTGG 
CCACCCCCCT ACAGACCGCG CAGAGACGCT TTTGAAATTT CTACTGAAGG GCATTCTGGC 
CCTAGCAATA GGGCCCGCTG GGGCCCTCGC GGGGCCCGTT CTCACAACCC TCGGAACCCA 
GCGTCCACTG CCATGGGCAG CTCCGTGCCC GGCTACTGCC AGCCCATCAC CACTGTGACG 
GCTTCTGCCT CCGTGACTGT CGCCGTGCAC CCGCCGCCTG TCCCTGGGCC TGGGCGGAAC 
CCCCGAGGGG GACTCTGCCC AGGCTACCCT GAGACTGACC ACGGCCTGTT TGAGGACCCC 
CACGTGCCTT TCCACGTCCG GTGTGAGAGG AGGGATTCGA AGGTGGAAGT CATTGAGCTG 
CAGGACGTGG AATGCGAGGA GAGGCCCCGG GGAAGCAGCT CCAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001341847.1
CDS	906..5093
Translation	MASAGNAAEPQDRGGGGSGCIGAPGRPAGGGRRRRTGGLRRAAAPDRDYLHRPSYCDAAFALEQISKGKATGRKAPLWLRAKFQRLLFKLGCYIQKNCGKFLVVGLLIFGAFAVGLKAANLETNVEELWVEVGGRVSRELNYTRQKIGEEAMFNPQLMIQTPKEEGANVLTTEALLQHLDSALQASRVHVYMYNRQWKLEHLCYKSGELITETGYMDQIIEYLYPCLIITPLDCFWEGAKLQSGTAYLLGKPPLRWTNFDPLEFLEELKKINYQVDSWEEMLNKAEVGHGYMDRPCLNPADPDCPATAPNKNSTKPLDMALVLNGGCHGLSRKYMHWQEELIVGGTVKNSTGKLVSAHALQTMFQLMTPKQMYEHFKGYEYVSHINWNEDKAAAILEAWQRTYVEVVHQSVAQNSTQKVLSFTTTTLDDILKSFSDVSVIRVASGYLLMVLPFLALGVGVDDVFLLAHAFSETGQNKRIPFEDRTGECLKRTGASVALTSISNVTAFFMAALIPIPALRAFSLQAAVVVVFNFAMVLLIFPAILSMDLYRREDRRLDIFCCFTSPCVSRVIQVEPQAYTDTHDNTRYSPPPPYSSHSFAHETQITMQSTVQLRTEYDPHTHVYYTTAEPRSEISVQPVTVTQDTLSCQSPESTSSTRDLLSQFSDSSLHCLEPPCTKWTLSSFAEKHYAPFLLKPKAKVVVIFLFLGLLGVSLYGTTRVRDGLDLTDIVPRETREYDFIAAQFKYFSFYNMYIVTQKADYPNIQHLLYDLHRSFSNVKYVMLEENKQLPKMWLHYFRDWLQGLQDAFDSDWETGKIMPNNYKNGSDDGVLAYKLLVQTGSRDKPIDISQLTKQRLVDADGIINPSAFYIYLTAWVSNDPVAYAASQANIRPHRPEWVHDKADYMPETRLRIPAAEPIEYAQFPFYLNGLRDTSDFVEAIEKVRTICSNYTSLGLSSYPNGYPFLFWEQYIGLRHWLLLFISVVLACTFLVCAVFLLNPWTAGIIVMVLALMTVELFGMMGLIGIKLSAVPVVILIASVGIGVEFTVHVALAFLTAIGDKNRRAVLALEHMFAPVLDGAVSTLLGVLMLAGSEFDFIVRYFFAVLAILTILGVLNGLVLLPVLLSFFGPYPEVSPANGLNRLPTPSPEPPPSVVRFAMPPGHTHSGSDSSDSEYSSQTTVSGLSEELRHYEAQQGAGGPAHQVIVEATENPVFAHSTVVHPESRHHPPSNPRQQPHLDSGSLPPGRQGQQPRRDPPREGLWPPPYRPRRDAFEISTEGHSGPSNRARWGPRGARSHNPRNPASTAMGSSVPGYCQPITTVTASASVTVAVHPPPVPGPGRNPRGGLCPGYPETDHGLFEDPHVPFHVRCERRDSKVEVIELQDVECEERPRGSSSN

Target ORF information:

RefSeq Version	NM_001354918.2
Organism	Homo sapiens(human)
Definition	Homo sapiens patched 1 (PTCH1), transcript variant 8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001354918.2

ORF Insert Sequence:

ATGGCCTCGG CTGGTAACGC CGCCGAGCCC CAGGACCGCG GCGGCGGCGG CAGCGGCTGT 
ATCGGTGCCC CGGGACGGCC GGCTGGAGGC GGGAGGCGCA GACGGACGGG GGGGCTGCGC 
CGTGCTGCCG CGCCGGACCG GGACTATCTG CACCGGCCCA GCTACTGCGA CGCCGCCTTC 
GCTCTGGAGC AGATTTCCAA GGGGAAGGCT ACTGGCCGGA AAGCGCCGCT GTGGCTGAGA 
GCGAAGTTTC AGAGACTCTT ATTTAAACTG GGTTGTTACA TTCAAAAAAA CTGCGGCAAG 
TTCTTGGTTG TGGGCCTCCT CATATTTGGG GCCTTCGCGG TGGGATTAAA AGCAGCGAAC 
CTCGAGACCA ACGTGGAGGA GCTGTGGGTG GAAGTTGGAG GACGAGTAAG TCGTGAATTA 
AATTATACTC GCCAGAAGAT TGGAGAAGAG GCTATGTTTA ATCCTCAACT CATGATACAG 
ACCCCTAAAG AAGAAGGTGC TAATGTCCTG ACCACAGAAG CGCTCCTACA ACACCTGGAC 
TCGGCACTCC AGGCCAGCCG TGTCCATGTA TACATGTACA ACAGGCAGTG GAAATTGGAA 
CATTTGTGTT ACAAATCAGG AGAGCTTATC ACAGAAACAG GTTACATGGA TCAGATAATA 
GAATATCTTT ACCCTTGTTT GATTATTACA CCTTTGGACT GCTTCTGGGA AGGGGCGAAA 
TTACAGTCTG GGACAGCATA CCTCCTAGGT AAACCTCCTT TGCGGTGGAC AAACTTCGAC 
CCTTTGGAAT TCCTGGAAGA GTTAAAGAAA ATAAACTATC AAGTGGACAG CTGGGAGGAA 
ATGCTGAATA AGGCTGAGGT TGGTCATGGT TACATGGACC GCCCCTGCCT CAATCCGGCC 
GATCCAGACT GCCCCGCCAC AGCCCCCAAC AAAAATTCAA CCAAACCTCT TGATATGGCC 
CTTGTTTTGA ATGGTGGATG TCATGGCTTA TCCAGAAAGT ATATGCACTG GCAGGAGGAG 
TTGATTGTGG GTGGCACAGT CAAGAACAGC ACTGGAAAAC TCGTCAGCGC CCATGCCCTG 
CAGACCATGT TCCAGTTAAT GACTCCCAAG CAAATGTACG AGCACTTCAA GGGGTACGAG 
TATGTCTCAC ACATCAACTG GAACGAGGAC AAAGCGGCAG CCATCCTGGA GGCCTGGCAG 
AGGACATATG TGGAGGTGGT TCATCAGAGT GTCGCACAGA ACTCCACTCA AAAGGTGCTT 
TCCTTCACCA CCACGACCCT GGACGACATC CTGAAATCCT TCTCTGACGT CAGTGTCATC 
CGCGTGGCCA GCGGCTACTT ACTCATGGTT TTGCCATTTC TCGCTCTTGG TGTTGGTGTG 
GATGATGTTT TTCTTCTGGC CCACGCCTTC AGTGAAACAG GACAGAATAA AAGAATCCCT 
TTTGAGGACA GGACCGGGGA GTGCCTGAAG CGCACAGGAG CCAGCGTGGC CCTCACGTCC 
ATCAGCAATG TCACAGCCTT CTTCATGGCC GCGTTAATCC CAATTCCCGC TCTGCGGGCG 
TTCTCCCTCC AGGCAGCGGT AGTAGTGGTG TTCAATTTTG CCATGGTTCT GCTCATTTTT 
CCTGCAATTC TCAGCATGGA TTTATATCGA CGCGAGGACA GGAGACTGGA TATTTTCTGC 
TGTTTTACAA GCCCCTGCGT CAGCAGAGTG ATTCAGGTTG AACCTCAGGC CTACACCGAC 
ACACACGACA ATACCCGCTA CAGCCCCCCA CCTCCCTACA GCAGCCACAG CTTTGCCCAT 
GAAACGCAGA TTACCATGCA GTCCACTGTC CAGCTCCGCA CGGAGTACGA CCCCCACACG 
CACGTGTACT ACACCACCGC TGAGCCGCGC TCCGAGATCT CTGTGCAGCC CGTCACCGTG 
ACACAGGACA CCCTCAGCTG CCAGAGCCCA GAGAGCACCA GCTCCACAAG GGACCTGCTC 
TCCCAGTTCT CCGACTCCAG CCTCCACTGC CTCGAGCCCC CCTGTACGAA GTGGACACTC 
TCATCTTTTG CTGAGAAGCA CTATGCTCCT TTCCTCTTGA AACCAAAAGC CAAGGTAGTG 
GTGATCTTCC TTTTTCTGGG CTTGCTGGGG GTCAGCCTTT ATGGCACCAC CCGAGTGAGA 
GACGGGCTGG ACCTTACGGA CATTGTACCT CGGGAAACCA GAGAATATGA CTTTATTGCT 
GCACAATTCA AATACTTTTC TTTCTACAAC ATGTATATAG TCACCCAGAA AGCAGACTAC 
CCGAATATCC AGCACTTACT TTACGACCTA CACAGGAGTT TCAGTAACGT GAAGTATGTC 
ATGTTGGAAG AAAACAAACA GCTTCCCAAA ATGTGGCTGC ACTACTTCAG AGACTGGCTT 
CAGGGACTTC AGGATGCATT TGACAGTGAC TGGGAAACCG GGAAAATCAT GCCAAACAAT 
TACAAGAATG GATCAGACGA TGGAGTCCTT GCCTACAAAC TCCTGGTGCA AACCGGCAGC 
CGCGATAAGC CCATCGACAT CAGCCAGTTG ACTAAACAGC GTCTGGTGGA TGCAGATGGC 
ATCATTAATC CCAGCGCTTT CTACATCTAC CTGACGGCTT GGGTCAGCAA CGACCCCGTC 
GCGTATGCTG CCTCCCAGGC CAACATCCGG CCACACCGAC CAGAATGGGT CCACGACAAA 
GCCGACTACA TGCCTGAAAC AAGGCTGAGA ATCCCGGCAG CAGAGCCCAT CGAGTATGCC 
CAGTTCCCTT TCTACCTCAA CGGCTTGCGG GACACCTCAG ACTTTGTGGA GGCAATTGAA 
AAAGTAAGGA CCATCTGCAG CAACTATACG AGCCTGGGGC TGTCCAGTTA CCCCAACGGC 
TACCCCTTCC TCTTCTGGGA GCAGTACATC GGCCTCCGCC ACTGGCTGCT GCTGTTCATC 
AGCGTGGTGT TGGCCTGCAC ATTCCTCGTG TGCGCTGTCT TCCTTCTGAA CCCCTGGACG 
GCCGGGATCA TTGTGATGGT CCTGGCGCTG ATGACGGTCG AGCTGTTCGG CATGATGGGC 
CTCATCGGAA TCAAGCTCAG TGCCGTGCCC GTGGTCATCC TGATCGCTTC TGTTGGCATA 
GGAGTGGAGT TCACCGTTCA CGTTGCTTTG GCCTTTCTGA CGGCCATCGG CGACAAGAAC 
CGCAGGGCTG TGCTTGCCCT GGAGCACATG TTTGCACCCG TCCTGGATGG CGCCGTGTCC 
ACTCTGCTGG GAGTGCTGAT GCTGGCGGGA TCTGAGTTCG ACTTCATTGT CAGGTATTTC 
TTTGCTGTGC TGGCGATCCT CACCATCCTC GGCGTTCTCA ATGGGCTGGT TTTGCTTCCC 
GTGCTTTTGT CTTTCTTTGG ACCATATCCT GAGGTGTCTC CAGCCAACGG CTTGAACCGC 
CTGCCCACAC CCTCCCCTGA GCCACCCCCC AGCGTGGTCC GCTTCGCCAT GCCGCCCGGC 
CACACGCACA GCGGGTCTGA TTCCTCCGAC TCGGAGTATA GTTCCCAGAC GACAGTGTCA 
GGCCTCAGCG AGGAGCTTCG GCACTACGAG GCCCAGCAGG GCGCGGGAGG CCCTGCCCAC 
CAAGTGATCG TGGAAGCCAC AGAAAACCCC GTCTTCGCCC ACTCCACTGT GGTCCATCCC 
GAATCCAGGC ATCACCCACC CTCGAACCCG AGACAGCAGC CCCACCTGGA CTCAGGGTCC 
CTGCCTCCCG GACGGCAAGG CCAGCAGCCC CGCAGGGACC CCCCCAGAGA AGGCTTGTGG 
CCACCCCCCT ACAGACCGCG CAGAGACGCT TTTGAAATTT CTACTGAAGG GCATTCTGGC 
CCTAGCAATA GGGCCCGCTG GGGCCCTCGC GGGGCCCGTT CTCACAACCC TCGGAACCCA 
GCGTCCACTG CCATGGGCAG CTCCGTGCCC GGCTACTGCC AGCCCATCAC CACTGTGACG 
GCTTCTGCCT CCGTGACTGT CGCCGTGCAC CCGCCGCCTG TCCCTGGGCC TGGGCGGAAC 
CCCCGAGGGG GACTCTGCCC AGGCTACCCT GAGACTGACC ACGGCCTGTT TGAGGACCCC 
CACGTGCCTT TCCACGTCCG GTGTGAGAGG AGGGATTCGA AGGTGGAAGT CATTGAGCTG 
CAGGACGTGG AATGCGAGGA GAGGCCCCGG GGAAGCAGCT CCAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PTCH1 ( NM_001083603.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001077072.1 Homo sapiens patched 1 (PTCH1), transcript variant 1a', mRNA.

Price & Availability↑

CloneID	OHu17397
Clone ID Related Accession (Same CDS sequence)	NM_001083603.2 , NM_001083603.1
Accession Version	NM_001083603.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4341bp) Protein sequence SNP
Vector	pcDNA3.1⁺/C-(K)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-07-25
Organism	Homo sapiens(human)
Product	protein patched homolog 1 isoform L'
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AB189437.1, U59464.1, AB209495.1, BX117041.1, CR744004.1 and AI417736.1. On Aug 29, 2017 this sequence version replaced NM_001083603.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGAACTGC TTAATAGAAA CAGGCTTGTA ATTGTGAGTC CGCGCTGCAC TCCGCCGAAA 
GCCTCCGGCG GCCCAGCGCG CCGGGGTTTT TACACTTTCC GTTCCTTTTG TAAAGACGGA 
GGAGGAGGAG AAGAAGAAGA AGAAAACGGA GGAGAAGAAA AAGACGACAG GGGAGACAAA 
GAGACCCGCA GCGACAAGGG GAAGGCTACT GGCCGGAAAG CGCCGCTGTG GCTGAGAGCG 
AAGTTTCAGA GACTCTTATT TAAACTGGGT TGTTACATTC AAAAAAACTG CGGCAAGTTC 
TTGGTTGTGG GCCTCCTCAT ATTTGGGGCC TTCGCGGTGG GATTAAAAGC AGCGAACCTC 
GAGACCAACG TGGAGGAGCT GTGGGTGGAA GTTGGAGGAC GAGTAAGTCG TGAATTAAAT 
TATACTCGCC AGAAGATTGG AGAAGAGGCT ATGTTTAATC CTCAACTCAT GATACAGACC 
CCTAAAGAAG AAGGTGCTAA TGTCCTGACC ACAGAAGCGC TCCTACAACA CCTGGACTCG 
GCACTCCAGG CCAGCCGTGT CCATGTATAC ATGTACAACA GGCAGTGGAA ATTGGAACAT 
TTGTGTTACA AATCAGGAGA GCTTATCACA GAAACAGGTT ACATGGATCA GATAATAGAA 
TATCTTTACC CTTGTTTGAT TATTACACCT TTGGACTGCT TCTGGGAAGG GGCGAAATTA 
CAGTCTGGGA CAGCATACCT CCTAGGTAAA CCTCCTTTGC GGTGGACAAA CTTCGACCCT 
TTGGAATTCC TGGAAGAGTT AAAGAAAATA AACTATCAAG TGGACAGCTG GGAGGAAATG 
CTGAATAAGG CTGAGGTTGG TCATGGTTAC ATGGACCGCC CCTGCCTCAA TCCGGCCGAT 
CCAGACTGCC CCGCCACAGC CCCCAACAAA AATTCAACCA AACCTCTTGA TATGGCCCTT 
GTTTTGAATG GTGGATGTCA TGGCTTATCC AGAAAGTATA TGCACTGGCA GGAGGAGTTG 
ATTGTGGGTG GCACAGTCAA GAACAGCACT GGAAAACTCG TCAGCGCCCA TGCCCTGCAG 
ACCATGTTCC AGTTAATGAC TCCCAAGCAA ATGTACGAGC ACTTCAAGGG GTACGAGTAT 
GTCTCACACA TCAACTGGAA CGAGGACAAA GCGGCAGCCA TCCTGGAGGC CTGGCAGAGG 
ACATATGTGG AGGTGGTTCA TCAGAGTGTC GCACAGAACT CCACTCAAAA GGTGCTTTCC 
TTCACCACCA CGACCCTGGA CGACATCCTG AAATCCTTCT CTGACGTCAG TGTCATCCGC 
GTGGCCAGCG GCTACTTACT CATGCTCGCC TATGCCTGTC TAACCATGCT GCGCTGGGAC 
TGCTCCAAGT CCCAGGGTGC CGTGGGGCTG GCTGGCGTCC TGCTGGTTGC ACTGTCAGTG 
GCTGCAGGAC TGGGCCTGTG CTCATTGATC GGAATTTCCT TTAACGCTGC AACAACTCAG 
GTTTTGCCAT TTCTCGCTCT TGGTGTTGGT GTGGATGATG TTTTTCTTCT GGCCCACGCC 
TTCAGTGAAA CAGGACAGAA TAAAAGAATC CCTTTTGAGG ACAGGACCGG GGAGTGCCTG 
AAGCGCACAG GAGCCAGCGT GGCCCTCACG TCCATCAGCA ATGTCACAGC CTTCTTCATG 
GCCGCGTTAA TCCCAATTCC CGCTCTGCGG GCGTTCTCCC TCCAGGCAGC GGTAGTAGTG 
GTGTTCAATT TTGCCATGGT TCTGCTCATT TTTCCTGCAA TTCTCAGCAT GGATTTATAT 
CGACGCGAGG ACAGGAGACT GGATATTTTC TGCTGTTTTA CAAGCCCCTG CGTCAGCAGA 
GTGATTCAGG TTGAACCTCA GGCCTACACC GACACACACG ACAATACCCG CTACAGCCCC 
CCACCTCCCT ACAGCAGCCA CAGCTTTGCC CATGAAACGC AGATTACCAT GCAGTCCACT 
GTCCAGCTCC GCACGGAGTA CGACCCCCAC ACGCACGTGT ACTACACCAC CGCTGAGCCG 
CGCTCCGAGA TCTCTGTGCA GCCCGTCACC GTGACACAGG ACACCCTCAG CTGCCAGAGC 
CCAGAGAGCA CCAGCTCCAC AAGGGACCTG CTCTCCCAGT TCTCCGACTC CAGCCTCCAC 
TGCCTCGAGC CCCCCTGTAC GAAGTGGACA CTCTCATCTT TTGCTGAGAA GCACTATGCT 
CCTTTCCTCT TGAAACCAAA AGCCAAGGTA GTGGTGATCT TCCTTTTTCT GGGCTTGCTG 
GGGGTCAGCC TTTATGGCAC CACCCGAGTG AGAGACGGGC TGGACCTTAC GGACATTGTA 
CCTCGGGAAA CCAGAGAATA TGACTTTATT GCTGCACAAT TCAAATACTT TTCTTTCTAC 
AACATGTATA TAGTCACCCA GAAAGCAGAC TACCCGAATA TCCAGCACTT ACTTTACGAC 
CTACACAGGA GTTTCAGTAA CGTGAAGTAT GTCATGTTGG AAGAAAACAA ACAGCTTCCC 
AAAATGTGGC TGCACTACTT CAGAGACTGG CTTCAGGGAC TTCAGGATGC ATTTGACAGT 
GACTGGGAAA CCGGGAAAAT CATGCCAAAC AATTACAAGA ATGGATCAGA CGATGGAGTC 
CTTGCCTACA AACTCCTGGT GCAAACCGGC AGCCGCGATA AGCCCATCGA CATCAGCCAG 
TTGACTAAAC AGCGTCTGGT GGATGCAGAT GGCATCATTA ATCCCAGCGC TTTCTACATC 
TACCTGACGG CTTGGGTCAG CAACGACCCC GTCGCGTATG CTGCCTCCCA GGCCAACATC 
CGGCCACACC GACCAGAATG GGTCCACGAC AAAGCCGACT ACATGCCTGA AACAAGGCTG 
AGAATCCCGG CAGCAGAGCC CATCGAGTAT GCCCAGTTCC CTTTCTACCT CAACGGCTTG 
CGGGACACCT CAGACTTTGT GGAGGCAATT GAAAAAGTAA GGACCATCTG CAGCAACTAT 
ACGAGCCTGG GGCTGTCCAG TTACCCCAAC GGCTACCCCT TCCTCTTCTG GGAGCAGTAC 
ATCGGCCTCC GCCACTGGCT GCTGCTGTTC ATCAGCGTGG TGTTGGCCTG CACATTCCTC 
GTGTGCGCTG TCTTCCTTCT GAACCCCTGG ACGGCCGGGA TCATTGTGAT GGTCCTGGCG 
CTGATGACGG TCGAGCTGTT CGGCATGATG GGCCTCATCG GAATCAAGCT CAGTGCCGTG 
CCCGTGGTCA TCCTGATCGC TTCTGTTGGC ATAGGAGTGG AGTTCACCGT TCACGTTGCT 
TTGGCCTTTC TGACGGCCAT CGGCGACAAG AACCGCAGGG CTGTGCTTGC CCTGGAGCAC 
ATGTTTGCAC CCGTCCTGGA TGGCGCCGTG TCCACTCTGC TGGGAGTGCT GATGCTGGCG 
GGATCTGAGT TCGACTTCAT TGTCAGGTAT TTCTTTGCTG TGCTGGCGAT CCTCACCATC 
CTCGGCGTTC TCAATGGGCT GGTTTTGCTT CCCGTGCTTT TGTCTTTCTT TGGACCATAT 
CCTGAGGTGT CTCCAGCCAA CGGCTTGAAC CGCCTGCCCA CACCCTCCCC TGAGCCACCC 
CCCAGCGTGG TCCGCTTCGC CATGCCGCCC GGCCACACGC ACAGCGGGTC TGATTCCTCC 
GACTCGGAGT ATAGTTCCCA GACGACAGTG TCAGGCCTCA GCGAGGAGCT TCGGCACTAC 
GAGGCCCAGC AGGGCGCGGG AGGCCCTGCC CACCAAGTGA TCGTGGAAGC CACAGAAAAC 
CCCGTCTTCG CCCACTCCAC TGTGGTCCAT CCCGAATCCA GGCATCACCC ACCCTCGAAC 
CCGAGACAGC AGCCCCACCT GGACTCAGGG TCCCTGCCTC CCGGACGGCA AGGCCAGCAG 
CCCCGCAGGG ACCCCCCCAG AGAAGGCTTG TGGCCACCCC CCTACAGACC GCGCAGAGAC 
GCTTTTGAAA TTTCTACTGA AGGGCATTCT GGCCCTAGCA ATAGGGCCCG CTGGGGCCCT 
CGCGGGGCCC GTTCTCACAA CCCTCGGAAC CCAGCGTCCA CTGCCATGGG CAGCTCCGTG 
CCCGGCTACT GCCAGCCCAT CACCACTGTG ACGGCTTCTG CCTCCGTGAC TGTCGCCGTG 
CACCCGCCGC CTGTCCCTGG GCCTGGGCGG AACCCCCGAG GGGGACTCTG CCCAGGCTAC 
CCTGAGACTG ACCACGGCCT GTTTGAGGAC CCCCACGTGC CTTTCCACGT CCGGTGTGAG 
AGGAGGGATT CGAAGGTGGA AGTCATTGAG CTGCAGGACG TGGAATGCGA GGAGAGGCCC 
CGGGGAAGCA GCTCCAACTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001077072.1
CDS	146..4486
Translation	MELLNRNRLVIVSPRCTPPKASGGPARRGFYTFRSFCKDGGGGEEEEENGGEEKDDRGDKETRSDKGKATGRKAPLWLRAKFQRLLFKLGCYIQKNCGKFLVVGLLIFGAFAVGLKAANLETNVEELWVEVGGRVSRELNYTRQKIGEEAMFNPQLMIQTPKEEGANVLTTEALLQHLDSALQASRVHVYMYNRQWKLEHLCYKSGELITETGYMDQIIEYLYPCLIITPLDCFWEGAKLQSGTAYLLGKPPLRWTNFDPLEFLEELKKINYQVDSWEEMLNKAEVGHGYMDRPCLNPADPDCPATAPNKNSTKPLDMALVLNGGCHGLSRKYMHWQEELIVGGTVKNSTGKLVSAHALQTMFQLMTPKQMYEHFKGYEYVSHINWNEDKAAAILEAWQRTYVEVVHQSVAQNSTQKVLSFTTTTLDDILKSFSDVSVIRVASGYLLMLAYACLTMLRWDCSKSQGAVGLAGVLLVALSVAAGLGLCSLIGISFNAATTQVLPFLALGVGVDDVFLLAHAFSETGQNKRIPFEDRTGECLKRTGASVALTSISNVTAFFMAALIPIPALRAFSLQAAVVVVFNFAMVLLIFPAILSMDLYRREDRRLDIFCCFTSPCVSRVIQVEPQAYTDTHDNTRYSPPPPYSSHSFAHETQITMQSTVQLRTEYDPHTHVYYTTAEPRSEISVQPVTVTQDTLSCQSPESTSSTRDLLSQFSDSSLHCLEPPCTKWTLSSFAEKHYAPFLLKPKAKVVVIFLFLGLLGVSLYGTTRVRDGLDLTDIVPRETREYDFIAAQFKYFSFYNMYIVTQKADYPNIQHLLYDLHRSFSNVKYVMLEENKQLPKMWLHYFRDWLQGLQDAFDSDWETGKIMPNNYKNGSDDGVLAYKLLVQTGSRDKPIDISQLTKQRLVDADGIINPSAFYIYLTAWVSNDPVAYAASQANIRPHRPEWVHDKADYMPETRLRIPAAEPIEYAQFPFYLNGLRDTSDFVEAIEKVRTICSNYTSLGLSSYPNGYPFLFWEQYIGLRHWLLLFISVVLACTFLVCAVFLLNPWTAGIIVMVLALMTVELFGMMGLIGIKLSAVPVVILIASVGIGVEFTVHVALAFLTAIGDKNRRAVLALEHMFAPVLDGAVSTLLGVLMLAGSEFDFIVRYFFAVLAILTILGVLNGLVLLPVLLSFFGPYPEVSPANGLNRLPTPSPEPPPSVVRFAMPPGHTHSGSDSSDSEYSSQTTVSGLSEELRHYEAQQGAGGPAHQVIVEATENPVFAHSTVVHPESRHHPPSNPRQQPHLDSGSLPPGRQGQQPRRDPPREGLWPPPYRPRRDAFEISTEGHSGPSNRARWGPRGARSHNPRNPASTAMGSSVPGYCQPITTVTASASVTVAVHPPPVPGPGRNPRGGLCPGYPETDHGLFEDPHVPFHVRCERRDSKVEVIELQDVECEERPRGSSSN

Target ORF information:

RefSeq Version	NM_001083603.2
Organism	Homo sapiens(human)
Definition	Homo sapiens patched 1 (PTCH1), transcript variant 1a', mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001083603.2

ORF Insert Sequence:

ATGGAACTGC TTAATAGAAA CAGGCTTGTA ATTGTGAGTC CGCGCTGCAC TCCGCCGAAA 
GCCTCCGGCG GCCCAGCGCG CCGGGGTTTT TACACTTTCC GTTCCTTTTG TAAAGACGGA 
GGAGGAGGAG AAGAAGAAGA AGAAAACGGA GGAGAAGAAA AAGACGACAG GGGAGACAAA 
GAGACCCGCA GCGACAAGGG GAAGGCTACT GGCCGGAAAG CGCCGCTGTG GCTGAGAGCG 
AAGTTTCAGA GACTCTTATT TAAACTGGGT TGTTACATTC AAAAAAACTG CGGCAAGTTC 
TTGGTTGTGG GCCTCCTCAT ATTTGGGGCC TTCGCGGTGG GATTAAAAGC AGCGAACCTC 
GAGACCAACG TGGAGGAGCT GTGGGTGGAA GTTGGAGGAC GAGTAAGTCG TGAATTAAAT 
TATACTCGCC AGAAGATTGG AGAAGAGGCT ATGTTTAATC CTCAACTCAT GATACAGACC 
CCTAAAGAAG AAGGTGCTAA TGTCCTGACC ACAGAAGCGC TCCTACAACA CCTGGACTCG 
GCACTCCAGG CCAGCCGTGT CCATGTATAC ATGTACAACA GGCAGTGGAA ATTGGAACAT 
TTGTGTTACA AATCAGGAGA GCTTATCACA GAAACAGGTT ACATGGATCA GATAATAGAA 
TATCTTTACC CTTGTTTGAT TATTACACCT TTGGACTGCT TCTGGGAAGG GGCGAAATTA 
CAGTCTGGGA CAGCATACCT CCTAGGTAAA CCTCCTTTGC GGTGGACAAA CTTCGACCCT 
TTGGAATTCC TGGAAGAGTT AAAGAAAATA AACTATCAAG TGGACAGCTG GGAGGAAATG 
CTGAATAAGG CTGAGGTTGG TCATGGTTAC ATGGACCGCC CCTGCCTCAA TCCGGCCGAT 
CCAGACTGCC CCGCCACAGC CCCCAACAAA AATTCAACCA AACCTCTTGA TATGGCCCTT 
GTTTTGAATG GTGGATGTCA TGGCTTATCC AGAAAGTATA TGCACTGGCA GGAGGAGTTG 
ATTGTGGGTG GCACAGTCAA GAACAGCACT GGAAAACTCG TCAGCGCCCA TGCCCTGCAG 
ACCATGTTCC AGTTAATGAC TCCCAAGCAA ATGTACGAGC ACTTCAAGGG GTACGAGTAT 
GTCTCACACA TCAACTGGAA CGAGGACAAA GCGGCAGCCA TCCTGGAGGC CTGGCAGAGG 
ACATATGTGG AGGTGGTTCA TCAGAGTGTC GCACAGAACT CCACTCAAAA GGTGCTTTCC 
TTCACCACCA CGACCCTGGA CGACATCCTG AAATCCTTCT CTGACGTCAG TGTCATCCGC 
GTGGCCAGCG GCTACTTACT CATGCTCGCC TATGCCTGTC TAACCATGCT GCGCTGGGAC 
TGCTCCAAGT CCCAGGGTGC CGTGGGGCTG GCTGGCGTCC TGCTGGTTGC ACTGTCAGTG 
GCTGCAGGAC TGGGCCTGTG CTCATTGATC GGAATTTCCT TTAACGCTGC AACAACTCAG 
GTTTTGCCAT TTCTCGCTCT TGGTGTTGGT GTGGATGATG TTTTTCTTCT GGCCCACGCC 
TTCAGTGAAA CAGGACAGAA TAAAAGAATC CCTTTTGAGG ACAGGACCGG GGAGTGCCTG 
AAGCGCACAG GAGCCAGCGT GGCCCTCACG TCCATCAGCA ATGTCACAGC CTTCTTCATG 
GCCGCGTTAA TCCCAATTCC CGCTCTGCGG GCGTTCTCCC TCCAGGCAGC GGTAGTAGTG 
GTGTTCAATT TTGCCATGGT TCTGCTCATT TTTCCTGCAA TTCTCAGCAT GGATTTATAT 
CGACGCGAGG ACAGGAGACT GGATATTTTC TGCTGTTTTA CAAGCCCCTG CGTCAGCAGA 
GTGATTCAGG TTGAACCTCA GGCCTACACC GACACACACG ACAATACCCG CTACAGCCCC 
CCACCTCCCT ACAGCAGCCA CAGCTTTGCC CATGAAACGC AGATTACCAT GCAGTCCACT 
GTCCAGCTCC GCACGGAGTA CGACCCCCAC ACGCACGTGT ACTACACCAC CGCTGAGCCG 
CGCTCCGAGA TCTCTGTGCA GCCCGTCACC GTGACACAGG ACACCCTCAG CTGCCAGAGC 
CCAGAGAGCA CCAGCTCCAC AAGGGACCTG CTCTCCCAGT TCTCCGACTC CAGCCTCCAC 
TGCCTCGAGC CCCCCTGTAC GAAGTGGACA CTCTCATCTT TTGCTGAGAA GCACTATGCT 
CCTTTCCTCT TGAAACCAAA AGCCAAGGTA GTGGTGATCT TCCTTTTTCT GGGCTTGCTG 
GGGGTCAGCC TTTATGGCAC CACCCGAGTG AGAGACGGGC TGGACCTTAC GGACATTGTA 
CCTCGGGAAA CCAGAGAATA TGACTTTATT GCTGCACAAT TCAAATACTT TTCTTTCTAC 
AACATGTATA TAGTCACCCA GAAAGCAGAC TACCCGAATA TCCAGCACTT ACTTTACGAC 
CTACACAGGA GTTTCAGTAA CGTGAAGTAT GTCATGTTGG AAGAAAACAA ACAGCTTCCC 
AAAATGTGGC TGCACTACTT CAGAGACTGG CTTCAGGGAC TTCAGGATGC ATTTGACAGT 
GACTGGGAAA CCGGGAAAAT CATGCCAAAC AATTACAAGA ATGGATCAGA CGATGGAGTC 
CTTGCCTACA AACTCCTGGT GCAAACCGGC AGCCGCGATA AGCCCATCGA CATCAGCCAG 
TTGACTAAAC AGCGTCTGGT GGATGCAGAT GGCATCATTA ATCCCAGCGC TTTCTACATC 
TACCTGACGG CTTGGGTCAG CAACGACCCC GTCGCGTATG CTGCCTCCCA GGCCAACATC 
CGGCCACACC GACCAGAATG GGTCCACGAC AAAGCCGACT ACATGCCTGA AACAAGGCTG 
AGAATCCCGG CAGCAGAGCC CATCGAGTAT GCCCAGTTCC CTTTCTACCT CAACGGCTTG 
CGGGACACCT CAGACTTTGT GGAGGCAATT GAAAAAGTAA GGACCATCTG CAGCAACTAT 
ACGAGCCTGG GGCTGTCCAG TTACCCCAAC GGCTACCCCT TCCTCTTCTG GGAGCAGTAC 
ATCGGCCTCC GCCACTGGCT GCTGCTGTTC ATCAGCGTGG TGTTGGCCTG CACATTCCTC 
GTGTGCGCTG TCTTCCTTCT GAACCCCTGG ACGGCCGGGA TCATTGTGAT GGTCCTGGCG 
CTGATGACGG TCGAGCTGTT CGGCATGATG GGCCTCATCG GAATCAAGCT CAGTGCCGTG 
CCCGTGGTCA TCCTGATCGC TTCTGTTGGC ATAGGAGTGG AGTTCACCGT TCACGTTGCT 
TTGGCCTTTC TGACGGCCAT CGGCGACAAG AACCGCAGGG CTGTGCTTGC CCTGGAGCAC 
ATGTTTGCAC CCGTCCTGGA TGGCGCCGTG TCCACTCTGC TGGGAGTGCT GATGCTGGCG 
GGATCTGAGT TCGACTTCAT TGTCAGGTAT TTCTTTGCTG TGCTGGCGAT CCTCACCATC 
CTCGGCGTTC TCAATGGGCT GGTTTTGCTT CCCGTGCTTT TGTCTTTCTT TGGACCATAT 
CCTGAGGTGT CTCCAGCCAA CGGCTTGAAC CGCCTGCCCA CACCCTCCCC TGAGCCACCC 
CCCAGCGTGG TCCGCTTCGC CATGCCGCCC GGCCACACGC ACAGCGGGTC TGATTCCTCC 
GACTCGGAGT ATAGTTCCCA GACGACAGTG TCAGGCCTCA GCGAGGAGCT TCGGCACTAC 
GAGGCCCAGC AGGGCGCGGG AGGCCCTGCC CACCAAGTGA TCGTGGAAGC CACAGAAAAC 
CCCGTCTTCG CCCACTCCAC TGTGGTCCAT CCCGAATCCA GGCATCACCC ACCCTCGAAC 
CCGAGACAGC AGCCCCACCT GGACTCAGGG TCCCTGCCTC CCGGACGGCA AGGCCAGCAG 
CCCCGCAGGG ACCCCCCCAG AGAAGGCTTG TGGCCACCCC CCTACAGACC GCGCAGAGAC 
GCTTTTGAAA TTTCTACTGA AGGGCATTCT GGCCCTAGCA ATAGGGCCCG CTGGGGCCCT 
CGCGGGGCCC GTTCTCACAA CCCTCGGAAC CCAGCGTCCA CTGCCATGGG CAGCTCCGTG 
CCCGGCTACT GCCAGCCCAT CACCACTGTG ACGGCTTCTG CCTCCGTGAC TGTCGCCGTG 
CACCCGCCGC CTGTCCCTGG GCCTGGGCGG AACCCCCGAG GGGGACTCTG CCCAGGCTAC 
CCTGAGACTG ACCACGGCCT GTTTGAGGAC CCCCACGTGC CTTTCCACGT CCGGTGTGAG 
AGGAGGGATT CGAAGGTGGA AGTCATTGAG CTGCAGGACG TGGAATGCGA GGAGAGGCCC 
CGGGGAAGCA GCTCCAACTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PTCH1 ( NM_001083603.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001077072.1 Homo sapiens patched 1 (PTCH1), transcript variant 1a', mRNA.

Price & Availability↑

CloneID	OHu17397
Clone ID Related Accession (Same CDS sequence)	NM_001083603.2 , NM_001083603.1
Accession Version	NM_001083603.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4341bp) Protein sequence SNP
Vector	pcDNA3.1⁺/C-(K)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-04-24
Organism	Homo sapiens(Human)
Product	protein patched homolog 1 isoform L'
Comment	REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AB189437.1, U59464.1, AB209495.1, BX117041.1 and CR744004.1. Transcript Variant: This variant (1a') differs in the 5' UTR and has multiple differences in the 5' coding region, compared to variant 1b. These differences result in a protein (isoform L') with a shorter N-terminus and distinct translation initiation site, compared to isoform L. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGAACTGC TTAATAGAAA CAGGCTTGTA ATTGTGAGTC CGCGCTGCAC TCCGCCGAAA 
GCCTCCGGCG GCCCAGCGCG CCGGGGTTTT TACACTTTCC GTTCCTTTTG TAAAGACGGA 
GGAGGAGGAG AAGAAGAAGA AGAAAACGGA GGAGAAGAAA AAGACGACAG GGGAGACAAA 
GAGACCCGCA GCGACAAGGG GAAGGCTACT GGCCGGAAAG CGCCGCTGTG GCTGAGAGCG 
AAGTTTCAGA GACTCTTATT TAAACTGGGT TGTTACATTC AAAAAAACTG CGGCAAGTTC 
TTGGTTGTGG GCCTCCTCAT ATTTGGGGCC TTCGCGGTGG GATTAAAAGC AGCGAACCTC 
GAGACCAACG TGGAGGAGCT GTGGGTGGAA GTTGGAGGAC GAGTAAGTCG TGAATTAAAT 
TATACTCGCC AGAAGATTGG AGAAGAGGCT ATGTTTAATC CTCAACTCAT GATACAGACC 
CCTAAAGAAG AAGGTGCTAA TGTCCTGACC ACAGAAGCGC TCCTACAACA CCTGGACTCG 
GCACTCCAGG CCAGCCGTGT CCATGTATAC ATGTACAACA GGCAGTGGAA ATTGGAACAT 
TTGTGTTACA AATCAGGAGA GCTTATCACA GAAACAGGTT ACATGGATCA GATAATAGAA 
TATCTTTACC CTTGTTTGAT TATTACACCT TTGGACTGCT TCTGGGAAGG GGCGAAATTA 
CAGTCTGGGA CAGCATACCT CCTAGGTAAA CCTCCTTTGC GGTGGACAAA CTTCGACCCT 
TTGGAATTCC TGGAAGAGTT AAAGAAAATA AACTATCAAG TGGACAGCTG GGAGGAAATG 
CTGAATAAGG CTGAGGTTGG TCATGGTTAC ATGGACCGCC CCTGCCTCAA TCCGGCCGAT 
CCAGACTGCC CCGCCACAGC CCCCAACAAA AATTCAACCA AACCTCTTGA TATGGCCCTT 
GTTTTGAATG GTGGATGTCA TGGCTTATCC AGAAAGTATA TGCACTGGCA GGAGGAGTTG 
ATTGTGGGTG GCACAGTCAA GAACAGCACT GGAAAACTCG TCAGCGCCCA TGCCCTGCAG 
ACCATGTTCC AGTTAATGAC TCCCAAGCAA ATGTACGAGC ACTTCAAGGG GTACGAGTAT 
GTCTCACACA TCAACTGGAA CGAGGACAAA GCGGCAGCCA TCCTGGAGGC CTGGCAGAGG 
ACATATGTGG AGGTGGTTCA TCAGAGTGTC GCACAGAACT CCACTCAAAA GGTGCTTTCC 
TTCACCACCA CGACCCTGGA CGACATCCTG AAATCCTTCT CTGACGTCAG TGTCATCCGC 
GTGGCCAGCG GCTACTTACT CATGCTCGCC TATGCCTGTC TAACCATGCT GCGCTGGGAC 
TGCTCCAAGT CCCAGGGTGC CGTGGGGCTG GCTGGCGTCC TGCTGGTTGC ACTGTCAGTG 
GCTGCAGGAC TGGGCCTGTG CTCATTGATC GGAATTTCCT TTAACGCTGC AACAACTCAG 
GTTTTGCCAT TTCTCGCTCT TGGTGTTGGT GTGGATGATG TTTTTCTTCT GGCCCACGCC 
TTCAGTGAAA CAGGACAGAA TAAAAGAATC CCTTTTGAGG ACAGGACCGG GGAGTGCCTG 
AAGCGCACAG GAGCCAGCGT GGCCCTCACG TCCATCAGCA ATGTCACAGC CTTCTTCATG 
GCCGCGTTAA TCCCAATTCC CGCTCTGCGG GCGTTCTCCC TCCAGGCAGC GGTAGTAGTG 
GTGTTCAATT TTGCCATGGT TCTGCTCATT TTTCCTGCAA TTCTCAGCAT GGATTTATAT 
CGACGCGAGG ACAGGAGACT GGATATTTTC TGCTGTTTTA CAAGCCCCTG CGTCAGCAGA 
GTGATTCAGG TTGAACCTCA GGCCTACACC GACACACACG ACAATACCCG CTACAGCCCC 
CCACCTCCCT ACAGCAGCCA CAGCTTTGCC CATGAAACGC AGATTACCAT GCAGTCCACT 
GTCCAGCTCC GCACGGAGTA CGACCCCCAC ACGCACGTGT ACTACACCAC CGCTGAGCCG 
CGCTCCGAGA TCTCTGTGCA GCCCGTCACC GTGACACAGG ACACCCTCAG CTGCCAGAGC 
CCAGAGAGCA CCAGCTCCAC AAGGGACCTG CTCTCCCAGT TCTCCGACTC CAGCCTCCAC 
TGCCTCGAGC CCCCCTGTAC GAAGTGGACA CTCTCATCTT TTGCTGAGAA GCACTATGCT 
CCTTTCCTCT TGAAACCAAA AGCCAAGGTA GTGGTGATCT TCCTTTTTCT GGGCTTGCTG 
GGGGTCAGCC TTTATGGCAC CACCCGAGTG AGAGACGGGC TGGACCTTAC GGACATTGTA 
CCTCGGGAAA CCAGAGAATA TGACTTTATT GCTGCACAAT TCAAATACTT TTCTTTCTAC 
AACATGTATA TAGTCACCCA GAAAGCAGAC TACCCGAATA TCCAGCACTT ACTTTACGAC 
CTACACAGGA GTTTCAGTAA CGTGAAGTAT GTCATGTTGG AAGAAAACAA ACAGCTTCCC 
AAAATGTGGC TGCACTACTT CAGAGACTGG CTTCAGGGAC TTCAGGATGC ATTTGACAGT 
GACTGGGAAA CCGGGAAAAT CATGCCAAAC AATTACAAGA ATGGATCAGA CGATGGAGTC 
CTTGCCTACA AACTCCTGGT GCAAACCGGC AGCCGCGATA AGCCCATCGA CATCAGCCAG 
TTGACTAAAC AGCGTCTGGT GGATGCAGAT GGCATCATTA ATCCCAGCGC TTTCTACATC 
TACCTGACGG CTTGGGTCAG CAACGACCCC GTCGCGTATG CTGCCTCCCA GGCCAACATC 
CGGCCACACC GACCAGAATG GGTCCACGAC AAAGCCGACT ACATGCCTGA AACAAGGCTG 
AGAATCCCGG CAGCAGAGCC CATCGAGTAT GCCCAGTTCC CTTTCTACCT CAACGGCTTG 
CGGGACACCT CAGACTTTGT GGAGGCAATT GAAAAAGTAA GGACCATCTG CAGCAACTAT 
ACGAGCCTGG GGCTGTCCAG TTACCCCAAC GGCTACCCCT TCCTCTTCTG GGAGCAGTAC 
ATCGGCCTCC GCCACTGGCT GCTGCTGTTC ATCAGCGTGG TGTTGGCCTG CACATTCCTC 
GTGTGCGCTG TCTTCCTTCT GAACCCCTGG ACGGCCGGGA TCATTGTGAT GGTCCTGGCG 
CTGATGACGG TCGAGCTGTT CGGCATGATG GGCCTCATCG GAATCAAGCT CAGTGCCGTG 
CCCGTGGTCA TCCTGATCGC TTCTGTTGGC ATAGGAGTGG AGTTCACCGT TCACGTTGCT 
TTGGCCTTTC TGACGGCCAT CGGCGACAAG AACCGCAGGG CTGTGCTTGC CCTGGAGCAC 
ATGTTTGCAC CCGTCCTGGA TGGCGCCGTG TCCACTCTGC TGGGAGTGCT GATGCTGGCG 
GGATCTGAGT TCGACTTCAT TGTCAGGTAT TTCTTTGCTG TGCTGGCGAT CCTCACCATC 
CTCGGCGTTC TCAATGGGCT GGTTTTGCTT CCCGTGCTTT TGTCTTTCTT TGGACCATAT 
CCTGAGGTGT CTCCAGCCAA CGGCTTGAAC CGCCTGCCCA CACCCTCCCC TGAGCCACCC 
CCCAGCGTGG TCCGCTTCGC CATGCCGCCC GGCCACACGC ACAGCGGGTC TGATTCCTCC 
GACTCGGAGT ATAGTTCCCA GACGACAGTG TCAGGCCTCA GCGAGGAGCT TCGGCACTAC 
GAGGCCCAGC AGGGCGCGGG AGGCCCTGCC CACCAAGTGA TCGTGGAAGC CACAGAAAAC 
CCCGTCTTCG CCCACTCCAC TGTGGTCCAT CCCGAATCCA GGCATCACCC ACCCTCGAAC 
CCGAGACAGC AGCCCCACCT GGACTCAGGG TCCCTGCCTC CCGGACGGCA AGGCCAGCAG 
CCCCGCAGGG ACCCCCCCAG AGAAGGCTTG TGGCCACCCC CCTACAGACC GCGCAGAGAC 
GCTTTTGAAA TTTCTACTGA AGGGCATTCT GGCCCTAGCA ATAGGGCCCG CTGGGGCCCT 
CGCGGGGCCC GTTCTCACAA CCCTCGGAAC CCAGCGTCCA CTGCCATGGG CAGCTCCGTG 
CCCGGCTACT GCCAGCCCAT CACCACTGTG ACGGCTTCTG CCTCCGTGAC TGTCGCCGTG 
CACCCGCCGC CTGTCCCTGG GCCTGGGCGG AACCCCCGAG GGGGACTCTG CCCAGGCTAC 
CCTGAGACTG ACCACGGCCT GTTTGAGGAC CCCCACGTGC CTTTCCACGT CCGGTGTGAG 
AGGAGGGATT CGAAGGTGGA AGTCATTGAG CTGCAGGACG TGGAATGCGA GGAGAGGCCC 
CGGGGAAGCA GCTCCAACTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001077072.1
CDS	146..4486
Translation	MELLNRNRLVIVSPRCTPPKASGGPARRGFYTFRSFCKDGGGGEEEEENGGEEKDDRGDKETRSDKGKATGRKAPLWLRAKFQRLLFKLGCYIQKNCGKFLVVGLLIFGAFAVGLKAANLETNVEELWVEVGGRVSRELNYTRQKIGEEAMFNPQLMIQTPKEEGANVLTTEALLQHLDSALQASRVHVYMYNRQWKLEHLCYKSGELITETGYMDQIIEYLYPCLIITPLDCFWEGAKLQSGTAYLLGKPPLRWTNFDPLEFLEELKKINYQVDSWEEMLNKAEVGHGYMDRPCLNPADPDCPATAPNKNSTKPLDMALVLNGGCHGLSRKYMHWQEELIVGGTVKNSTGKLVSAHALQTMFQLMTPKQMYEHFKGYEYVSHINWNEDKAAAILEAWQRTYVEVVHQSVAQNSTQKVLSFTTTTLDDILKSFSDVSVIRVASGYLLMLAYACLTMLRWDCSKSQGAVGLAGVLLVALSVAAGLGLCSLIGISFNAATTQVLPFLALGVGVDDVFLLAHAFSETGQNKRIPFEDRTGECLKRTGASVALTSISNVTAFFMAALIPIPALRAFSLQAAVVVVFNFAMVLLIFPAILSMDLYRREDRRLDIFCCFTSPCVSRVIQVEPQAYTDTHDNTRYSPPPPYSSHSFAHETQITMQSTVQLRTEYDPHTHVYYTTAEPRSEISVQPVTVTQDTLSCQSPESTSSTRDLLSQFSDSSLHCLEPPCTKWTLSSFAEKHYAPFLLKPKAKVVVIFLFLGLLGVSLYGTTRVRDGLDLTDIVPRETREYDFIAAQFKYFSFYNMYIVTQKADYPNIQHLLYDLHRSFSNVKYVMLEENKQLPKMWLHYFRDWLQGLQDAFDSDWETGKIMPNNYKNGSDDGVLAYKLLVQTGSRDKPIDISQLTKQRLVDADGIINPSAFYIYLTAWVSNDPVAYAASQANIRPHRPEWVHDKADYMPETRLRIPAAEPIEYAQFPFYLNGLRDTSDFVEAIEKVRTICSNYTSLGLSSYPNGYPFLFWEQYIGLRHWLLLFISVVLACTFLVCAVFLLNPWTAGIIVMVLALMTVELFGMMGLIGIKLSAVPVVILIASVGIGVEFTVHVALAFLTAIGDKNRRAVLALEHMFAPVLDGAVSTLLGVLMLAGSEFDFIVRYFFAVLAILTILGVLNGLVLLPVLLSFFGPYPEVSPANGLNRLPTPSPEPPPSVVRFAMPPGHTHSGSDSSDSEYSSQTTVSGLSEELRHYEAQQGAGGPAHQVIVEATENPVFAHSTVVHPESRHHPPSNPRQQPHLDSGSLPPGRQGQQPRRDPPREGLWPPPYRPRRDAFEISTEGHSGPSNRARWGPRGARSHNPRNPASTAMGSSVPGYCQPITTVTASASVTVAVHPPPVPGPGRNPRGGLCPGYPETDHGLFEDPHVPFHVRCERRDSKVEVIELQDVECEERPRGSSSN

Target ORF information:

RefSeq Version	NM_001083603.1
Organism	Homo sapiens(Human)
Definition	Homo sapiens patched 1 (PTCH1), transcript variant 1a', mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001083603.1

ORF Insert Sequence:

ATGGAACTGC TTAATAGAAA CAGGCTTGTA ATTGTGAGTC CGCGCTGCAC TCCGCCGAAA 
GCCTCCGGCG GCCCAGCGCG CCGGGGTTTT TACACTTTCC GTTCCTTTTG TAAAGACGGA 
GGAGGAGGAG AAGAAGAAGA AGAAAACGGA GGAGAAGAAA AAGACGACAG GGGAGACAAA 
GAGACCCGCA GCGACAAGGG GAAGGCTACT GGCCGGAAAG CGCCGCTGTG GCTGAGAGCG 
AAGTTTCAGA GACTCTTATT TAAACTGGGT TGTTACATTC AAAAAAACTG CGGCAAGTTC 
TTGGTTGTGG GCCTCCTCAT ATTTGGGGCC TTCGCGGTGG GATTAAAAGC AGCGAACCTC 
GAGACCAACG TGGAGGAGCT GTGGGTGGAA GTTGGAGGAC GAGTAAGTCG TGAATTAAAT 
TATACTCGCC AGAAGATTGG AGAAGAGGCT ATGTTTAATC CTCAACTCAT GATACAGACC 
CCTAAAGAAG AAGGTGCTAA TGTCCTGACC ACAGAAGCGC TCCTACAACA CCTGGACTCG 
GCACTCCAGG CCAGCCGTGT CCATGTATAC ATGTACAACA GGCAGTGGAA ATTGGAACAT 
TTGTGTTACA AATCAGGAGA GCTTATCACA GAAACAGGTT ACATGGATCA GATAATAGAA 
TATCTTTACC CTTGTTTGAT TATTACACCT TTGGACTGCT TCTGGGAAGG GGCGAAATTA 
CAGTCTGGGA CAGCATACCT CCTAGGTAAA CCTCCTTTGC GGTGGACAAA CTTCGACCCT 
TTGGAATTCC TGGAAGAGTT AAAGAAAATA AACTATCAAG TGGACAGCTG GGAGGAAATG 
CTGAATAAGG CTGAGGTTGG TCATGGTTAC ATGGACCGCC CCTGCCTCAA TCCGGCCGAT 
CCAGACTGCC CCGCCACAGC CCCCAACAAA AATTCAACCA AACCTCTTGA TATGGCCCTT 
GTTTTGAATG GTGGATGTCA TGGCTTATCC AGAAAGTATA TGCACTGGCA GGAGGAGTTG 
ATTGTGGGTG GCACAGTCAA GAACAGCACT GGAAAACTCG TCAGCGCCCA TGCCCTGCAG 
ACCATGTTCC AGTTAATGAC TCCCAAGCAA ATGTACGAGC ACTTCAAGGG GTACGAGTAT 
GTCTCACACA TCAACTGGAA CGAGGACAAA GCGGCAGCCA TCCTGGAGGC CTGGCAGAGG 
ACATATGTGG AGGTGGTTCA TCAGAGTGTC GCACAGAACT CCACTCAAAA GGTGCTTTCC 
TTCACCACCA CGACCCTGGA CGACATCCTG AAATCCTTCT CTGACGTCAG TGTCATCCGC 
GTGGCCAGCG GCTACTTACT CATGCTCGCC TATGCCTGTC TAACCATGCT GCGCTGGGAC 
TGCTCCAAGT CCCAGGGTGC CGTGGGGCTG GCTGGCGTCC TGCTGGTTGC ACTGTCAGTG 
GCTGCAGGAC TGGGCCTGTG CTCATTGATC GGAATTTCCT TTAACGCTGC AACAACTCAG 
GTTTTGCCAT TTCTCGCTCT TGGTGTTGGT GTGGATGATG TTTTTCTTCT GGCCCACGCC 
TTCAGTGAAA CAGGACAGAA TAAAAGAATC CCTTTTGAGG ACAGGACCGG GGAGTGCCTG 
AAGCGCACAG GAGCCAGCGT GGCCCTCACG TCCATCAGCA ATGTCACAGC CTTCTTCATG 
GCCGCGTTAA TCCCAATTCC CGCTCTGCGG GCGTTCTCCC TCCAGGCAGC GGTAGTAGTG 
GTGTTCAATT TTGCCATGGT TCTGCTCATT TTTCCTGCAA TTCTCAGCAT GGATTTATAT 
CGACGCGAGG ACAGGAGACT GGATATTTTC TGCTGTTTTA CAAGCCCCTG CGTCAGCAGA 
GTGATTCAGG TTGAACCTCA GGCCTACACC GACACACACG ACAATACCCG CTACAGCCCC 
CCACCTCCCT ACAGCAGCCA CAGCTTTGCC CATGAAACGC AGATTACCAT GCAGTCCACT 
GTCCAGCTCC GCACGGAGTA CGACCCCCAC ACGCACGTGT ACTACACCAC CGCTGAGCCG 
CGCTCCGAGA TCTCTGTGCA GCCCGTCACC GTGACACAGG ACACCCTCAG CTGCCAGAGC 
CCAGAGAGCA CCAGCTCCAC AAGGGACCTG CTCTCCCAGT TCTCCGACTC CAGCCTCCAC 
TGCCTCGAGC CCCCCTGTAC GAAGTGGACA CTCTCATCTT TTGCTGAGAA GCACTATGCT 
CCTTTCCTCT TGAAACCAAA AGCCAAGGTA GTGGTGATCT TCCTTTTTCT GGGCTTGCTG 
GGGGTCAGCC TTTATGGCAC CACCCGAGTG AGAGACGGGC TGGACCTTAC GGACATTGTA 
CCTCGGGAAA CCAGAGAATA TGACTTTATT GCTGCACAAT TCAAATACTT TTCTTTCTAC 
AACATGTATA TAGTCACCCA GAAAGCAGAC TACCCGAATA TCCAGCACTT ACTTTACGAC 
CTACACAGGA GTTTCAGTAA CGTGAAGTAT GTCATGTTGG AAGAAAACAA ACAGCTTCCC 
AAAATGTGGC TGCACTACTT CAGAGACTGG CTTCAGGGAC TTCAGGATGC ATTTGACAGT 
GACTGGGAAA CCGGGAAAAT CATGCCAAAC AATTACAAGA ATGGATCAGA CGATGGAGTC 
CTTGCCTACA AACTCCTGGT GCAAACCGGC AGCCGCGATA AGCCCATCGA CATCAGCCAG 
TTGACTAAAC AGCGTCTGGT GGATGCAGAT GGCATCATTA ATCCCAGCGC TTTCTACATC 
TACCTGACGG CTTGGGTCAG CAACGACCCC GTCGCGTATG CTGCCTCCCA GGCCAACATC 
CGGCCACACC GACCAGAATG GGTCCACGAC AAAGCCGACT ACATGCCTGA AACAAGGCTG 
AGAATCCCGG CAGCAGAGCC CATCGAGTAT GCCCAGTTCC CTTTCTACCT CAACGGCTTG 
CGGGACACCT CAGACTTTGT GGAGGCAATT GAAAAAGTAA GGACCATCTG CAGCAACTAT 
ACGAGCCTGG GGCTGTCCAG TTACCCCAAC GGCTACCCCT TCCTCTTCTG GGAGCAGTAC 
ATCGGCCTCC GCCACTGGCT GCTGCTGTTC ATCAGCGTGG TGTTGGCCTG CACATTCCTC 
GTGTGCGCTG TCTTCCTTCT GAACCCCTGG ACGGCCGGGA TCATTGTGAT GGTCCTGGCG 
CTGATGACGG TCGAGCTGTT CGGCATGATG GGCCTCATCG GAATCAAGCT CAGTGCCGTG 
CCCGTGGTCA TCCTGATCGC TTCTGTTGGC ATAGGAGTGG AGTTCACCGT TCACGTTGCT 
TTGGCCTTTC TGACGGCCAT CGGCGACAAG AACCGCAGGG CTGTGCTTGC CCTGGAGCAC 
ATGTTTGCAC CCGTCCTGGA TGGCGCCGTG TCCACTCTGC TGGGAGTGCT GATGCTGGCG 
GGATCTGAGT TCGACTTCAT TGTCAGGTAT TTCTTTGCTG TGCTGGCGAT CCTCACCATC 
CTCGGCGTTC TCAATGGGCT GGTTTTGCTT CCCGTGCTTT TGTCTTTCTT TGGACCATAT 
CCTGAGGTGT CTCCAGCCAA CGGCTTGAAC CGCCTGCCCA CACCCTCCCC TGAGCCACCC 
CCCAGCGTGG TCCGCTTCGC CATGCCGCCC GGCCACACGC ACAGCGGGTC TGATTCCTCC 
GACTCGGAGT ATAGTTCCCA GACGACAGTG TCAGGCCTCA GCGAGGAGCT TCGGCACTAC 
GAGGCCCAGC AGGGCGCGGG AGGCCCTGCC CACCAAGTGA TCGTGGAAGC CACAGAAAAC 
CCCGTCTTCG CCCACTCCAC TGTGGTCCAT CCCGAATCCA GGCATCACCC ACCCTCGAAC 
CCGAGACAGC AGCCCCACCT GGACTCAGGG TCCCTGCCTC CCGGACGGCA AGGCCAGCAG 
CCCCGCAGGG ACCCCCCCAG AGAAGGCTTG TGGCCACCCC CCTACAGACC GCGCAGAGAC 
GCTTTTGAAA TTTCTACTGA AGGGCATTCT GGCCCTAGCA ATAGGGCCCG CTGGGGCCCT 
CGCGGGGCCC GTTCTCACAA CCCTCGGAAC CCAGCGTCCA CTGCCATGGG CAGCTCCGTG 
CCCGGCTACT GCCAGCCCAT CACCACTGTG ACGGCTTCTG CCTCCGTGAC TGTCGCCGTG 
CACCCGCCGC CTGTCCCTGG GCCTGGGCGG AACCCCCGAG GGGGACTCTG CCCAGGCTAC 
CCTGAGACTG ACCACGGCCT GTTTGAGGAC CCCCACGTGC CTTTCCACGT CCGGTGTGAG 
AGGAGGGATT CGAAGGTGGA AGTCATTGAG CTGCAGGACG TGGAATGCGA GGAGAGGCCC 
CGGGGAAGCA GCTCCAACTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PTCH1 ( NM_001083602.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001077071.1 Homo sapiens patched 1 (PTCH1), transcript variant 1a, mRNA.

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CloneID	OHu19644
Clone ID Related Accession (Same CDS sequence)	NM_001083602.2 , NM_001083602.1
Accession Version	NM_001083602.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4146bp) Protein sequence SNP
Vector	pcDNA3.1⁺/C-(K)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-07-25
Organism	Homo sapiens(human)
Product	protein patched homolog 1 isoform M
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BC043542.1, AB189436.1, U59464.1, AB209495.1, BX117041.1, CR744004.1 and AI417736.1. On Aug 29, 2017 this sequence version replaced NM_001083602.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGGGAAGG CTACTGGCCG GAAAGCGCCG CTGTGGCTGA GAGCGAAGTT TCAGAGACTC 
TTATTTAAAC TGGGTTGTTA CATTCAAAAA AACTGCGGCA AGTTCTTGGT TGTGGGCCTC 
CTCATATTTG GGGCCTTCGC GGTGGGATTA AAAGCAGCGA ACCTCGAGAC CAACGTGGAG 
GAGCTGTGGG TGGAAGTTGG AGGACGAGTA AGTCGTGAAT TAAATTATAC TCGCCAGAAG 
ATTGGAGAAG AGGCTATGTT TAATCCTCAA CTCATGATAC AGACCCCTAA AGAAGAAGGT 
GCTAATGTCC TGACCACAGA AGCGCTCCTA CAACACCTGG ACTCGGCACT CCAGGCCAGC 
CGTGTCCATG TATACATGTA CAACAGGCAG TGGAAATTGG AACATTTGTG TTACAAATCA 
GGAGAGCTTA TCACAGAAAC AGGTTACATG GATCAGATAA TAGAATATCT TTACCCTTGT 
TTGATTATTA CACCTTTGGA CTGCTTCTGG GAAGGGGCGA AATTACAGTC TGGGACAGCA 
TACCTCCTAG GTAAACCTCC TTTGCGGTGG ACAAACTTCG ACCCTTTGGA ATTCCTGGAA 
GAGTTAAAGA AAATAAACTA TCAAGTGGAC AGCTGGGAGG AAATGCTGAA TAAGGCTGAG 
GTTGGTCATG GTTACATGGA CCGCCCCTGC CTCAATCCGG CCGATCCAGA CTGCCCCGCC 
ACAGCCCCCA ACAAAAATTC AACCAAACCT CTTGATATGG CCCTTGTTTT GAATGGTGGA 
TGTCATGGCT TATCCAGAAA GTATATGCAC TGGCAGGAGG AGTTGATTGT GGGTGGCACA 
GTCAAGAACA GCACTGGAAA ACTCGTCAGC GCCCATGCCC TGCAGACCAT GTTCCAGTTA 
ATGACTCCCA AGCAAATGTA CGAGCACTTC AAGGGGTACG AGTATGTCTC ACACATCAAC 
TGGAACGAGG ACAAAGCGGC AGCCATCCTG GAGGCCTGGC AGAGGACATA TGTGGAGGTG 
GTTCATCAGA GTGTCGCACA GAACTCCACT CAAAAGGTGC TTTCCTTCAC CACCACGACC 
CTGGACGACA TCCTGAAATC CTTCTCTGAC GTCAGTGTCA TCCGCGTGGC CAGCGGCTAC 
TTACTCATGC TCGCCTATGC CTGTCTAACC ATGCTGCGCT GGGACTGCTC CAAGTCCCAG 
GGTGCCGTGG GGCTGGCTGG CGTCCTGCTG GTTGCACTGT CAGTGGCTGC AGGACTGGGC 
CTGTGCTCAT TGATCGGAAT TTCCTTTAAC GCTGCAACAA CTCAGGTTTT GCCATTTCTC 
GCTCTTGGTG TTGGTGTGGA TGATGTTTTT CTTCTGGCCC ACGCCTTCAG TGAAACAGGA 
CAGAATAAAA GAATCCCTTT TGAGGACAGG ACCGGGGAGT GCCTGAAGCG CACAGGAGCC 
AGCGTGGCCC TCACGTCCAT CAGCAATGTC ACAGCCTTCT TCATGGCCGC GTTAATCCCA 
ATTCCCGCTC TGCGGGCGTT CTCCCTCCAG GCAGCGGTAG TAGTGGTGTT CAATTTTGCC 
ATGGTTCTGC TCATTTTTCC TGCAATTCTC AGCATGGATT TATATCGACG CGAGGACAGG 
AGACTGGATA TTTTCTGCTG TTTTACAAGC CCCTGCGTCA GCAGAGTGAT TCAGGTTGAA 
CCTCAGGCCT ACACCGACAC ACACGACAAT ACCCGCTACA GCCCCCCACC TCCCTACAGC 
AGCCACAGCT TTGCCCATGA AACGCAGATT ACCATGCAGT CCACTGTCCA GCTCCGCACG 
GAGTACGACC CCCACACGCA CGTGTACTAC ACCACCGCTG AGCCGCGCTC CGAGATCTCT 
GTGCAGCCCG TCACCGTGAC ACAGGACACC CTCAGCTGCC AGAGCCCAGA GAGCACCAGC 
TCCACAAGGG ACCTGCTCTC CCAGTTCTCC GACTCCAGCC TCCACTGCCT CGAGCCCCCC 
TGTACGAAGT GGACACTCTC ATCTTTTGCT GAGAAGCACT ATGCTCCTTT CCTCTTGAAA 
CCAAAAGCCA AGGTAGTGGT GATCTTCCTT TTTCTGGGCT TGCTGGGGGT CAGCCTTTAT 
GGCACCACCC GAGTGAGAGA CGGGCTGGAC CTTACGGACA TTGTACCTCG GGAAACCAGA 
GAATATGACT TTATTGCTGC ACAATTCAAA TACTTTTCTT TCTACAACAT GTATATAGTC 
ACCCAGAAAG CAGACTACCC GAATATCCAG CACTTACTTT ACGACCTACA CAGGAGTTTC 
AGTAACGTGA AGTATGTCAT GTTGGAAGAA AACAAACAGC TTCCCAAAAT GTGGCTGCAC 
TACTTCAGAG ACTGGCTTCA GGGACTTCAG GATGCATTTG ACAGTGACTG GGAAACCGGG 
AAAATCATGC CAAACAATTA CAAGAATGGA TCAGACGATG GAGTCCTTGC CTACAAACTC 
CTGGTGCAAA CCGGCAGCCG CGATAAGCCC ATCGACATCA GCCAGTTGAC TAAACAGCGT 
CTGGTGGATG CAGATGGCAT CATTAATCCC AGCGCTTTCT ACATCTACCT GACGGCTTGG 
GTCAGCAACG ACCCCGTCGC GTATGCTGCC TCCCAGGCCA ACATCCGGCC ACACCGACCA 
GAATGGGTCC ACGACAAAGC CGACTACATG CCTGAAACAA GGCTGAGAAT CCCGGCAGCA 
GAGCCCATCG AGTATGCCCA GTTCCCTTTC TACCTCAACG GCTTGCGGGA CACCTCAGAC 
TTTGTGGAGG CAATTGAAAA AGTAAGGACC ATCTGCAGCA ACTATACGAG CCTGGGGCTG 
TCCAGTTACC CCAACGGCTA CCCCTTCCTC TTCTGGGAGC AGTACATCGG CCTCCGCCAC 
TGGCTGCTGC TGTTCATCAG CGTGGTGTTG GCCTGCACAT TCCTCGTGTG CGCTGTCTTC 
CTTCTGAACC CCTGGACGGC CGGGATCATT GTGATGGTCC TGGCGCTGAT GACGGTCGAG 
CTGTTCGGCA TGATGGGCCT CATCGGAATC AAGCTCAGTG CCGTGCCCGT GGTCATCCTG 
ATCGCTTCTG TTGGCATAGG AGTGGAGTTC ACCGTTCACG TTGCTTTGGC CTTTCTGACG 
GCCATCGGCG ACAAGAACCG CAGGGCTGTG CTTGCCCTGG AGCACATGTT TGCACCCGTC 
CTGGATGGCG CCGTGTCCAC TCTGCTGGGA GTGCTGATGC TGGCGGGATC TGAGTTCGAC 
TTCATTGTCA GGTATTTCTT TGCTGTGCTG GCGATCCTCA CCATCCTCGG CGTTCTCAAT 
GGGCTGGTTT TGCTTCCCGT GCTTTTGTCT TTCTTTGGAC CATATCCTGA GGTGTCTCCA 
GCCAACGGCT TGAACCGCCT GCCCACACCC TCCCCTGAGC CACCCCCCAG CGTGGTCCGC 
TTCGCCATGC CGCCCGGCCA CACGCACAGC GGGTCTGATT CCTCCGACTC GGAGTATAGT 
TCCCAGACGA CAGTGTCAGG CCTCAGCGAG GAGCTTCGGC ACTACGAGGC CCAGCAGGGC 
GCGGGAGGCC CTGCCCACCA AGTGATCGTG GAAGCCACAG AAAACCCCGT CTTCGCCCAC 
TCCACTGTGG TCCATCCCGA ATCCAGGCAT CACCCACCCT CGAACCCGAG ACAGCAGCCC 
CACCTGGACT CAGGGTCCCT GCCTCCCGGA CGGCAAGGCC AGCAGCCCCG CAGGGACCCC 
CCCAGAGAAG GCTTGTGGCC ACCCCCCTAC AGACCGCGCA GAGACGCTTT TGAAATTTCT 
ACTGAAGGGC ATTCTGGCCC TAGCAATAGG GCCCGCTGGG GCCCTCGCGG GGCCCGTTCT 
CACAACCCTC GGAACCCAGC GTCCACTGCC ATGGGCAGCT CCGTGCCCGG CTACTGCCAG 
CCCATCACCA CTGTGACGGC TTCTGCCTCC GTGACTGTCG CCGTGCACCC GCCGCCTGTC 
CCTGGGCCTG GGCGGAACCC CCGAGGGGGA CTCTGCCCAG GCTACCCTGA GACTGACCAC 
GGCCTGTTTG AGGACCCCCA CGTGCCTTTC CACGTCCGGT GTGAGAGGAG GGATTCGAAG 
GTGGAAGTCA TTGAGCTGCA GGACGTGGAA TGCGAGGAGA GGCCCCGGGG AAGCAGCTCC 
AACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001077071.1
CDS	587..4732
Translation	MGKATGRKAPLWLRAKFQRLLFKLGCYIQKNCGKFLVVGLLIFGAFAVGLKAANLETNVEELWVEVGGRVSRELNYTRQKIGEEAMFNPQLMIQTPKEEGANVLTTEALLQHLDSALQASRVHVYMYNRQWKLEHLCYKSGELITETGYMDQIIEYLYPCLIITPLDCFWEGAKLQSGTAYLLGKPPLRWTNFDPLEFLEELKKINYQVDSWEEMLNKAEVGHGYMDRPCLNPADPDCPATAPNKNSTKPLDMALVLNGGCHGLSRKYMHWQEELIVGGTVKNSTGKLVSAHALQTMFQLMTPKQMYEHFKGYEYVSHINWNEDKAAAILEAWQRTYVEVVHQSVAQNSTQKVLSFTTTTLDDILKSFSDVSVIRVASGYLLMLAYACLTMLRWDCSKSQGAVGLAGVLLVALSVAAGLGLCSLIGISFNAATTQVLPFLALGVGVDDVFLLAHAFSETGQNKRIPFEDRTGECLKRTGASVALTSISNVTAFFMAALIPIPALRAFSLQAAVVVVFNFAMVLLIFPAILSMDLYRREDRRLDIFCCFTSPCVSRVIQVEPQAYTDTHDNTRYSPPPPYSSHSFAHETQITMQSTVQLRTEYDPHTHVYYTTAEPRSEISVQPVTVTQDTLSCQSPESTSSTRDLLSQFSDSSLHCLEPPCTKWTLSSFAEKHYAPFLLKPKAKVVVIFLFLGLLGVSLYGTTRVRDGLDLTDIVPRETREYDFIAAQFKYFSFYNMYIVTQKADYPNIQHLLYDLHRSFSNVKYVMLEENKQLPKMWLHYFRDWLQGLQDAFDSDWETGKIMPNNYKNGSDDGVLAYKLLVQTGSRDKPIDISQLTKQRLVDADGIINPSAFYIYLTAWVSNDPVAYAASQANIRPHRPEWVHDKADYMPETRLRIPAAEPIEYAQFPFYLNGLRDTSDFVEAIEKVRTICSNYTSLGLSSYPNGYPFLFWEQYIGLRHWLLLFISVVLACTFLVCAVFLLNPWTAGIIVMVLALMTVELFGMMGLIGIKLSAVPVVILIASVGIGVEFTVHVALAFLTAIGDKNRRAVLALEHMFAPVLDGAVSTLLGVLMLAGSEFDFIVRYFFAVLAILTILGVLNGLVLLPVLLSFFGPYPEVSPANGLNRLPTPSPEPPPSVVRFAMPPGHTHSGSDSSDSEYSSQTTVSGLSEELRHYEAQQGAGGPAHQVIVEATENPVFAHSTVVHPESRHHPPSNPRQQPHLDSGSLPPGRQGQQPRRDPPREGLWPPPYRPRRDAFEISTEGHSGPSNRARWGPRGARSHNPRNPASTAMGSSVPGYCQPITTVTASASVTVAVHPPPVPGPGRNPRGGLCPGYPETDHGLFEDPHVPFHVRCERRDSKVEVIELQDVECEERPRGSSSN

Target ORF information:

RefSeq Version	NM_001083602.2
Organism	Homo sapiens(human)
Definition	Homo sapiens patched 1 (PTCH1), transcript variant 1a, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001083602.2

ORF Insert Sequence:

ATGGGGAAGG CTACTGGCCG GAAAGCGCCG CTGTGGCTGA GAGCGAAGTT TCAGAGACTC 
TTATTTAAAC TGGGTTGTTA CATTCAAAAA AACTGCGGCA AGTTCTTGGT TGTGGGCCTC 
CTCATATTTG GGGCCTTCGC GGTGGGATTA AAAGCAGCGA ACCTCGAGAC CAACGTGGAG 
GAGCTGTGGG TGGAAGTTGG AGGACGAGTA AGTCGTGAAT TAAATTATAC TCGCCAGAAG 
ATTGGAGAAG AGGCTATGTT TAATCCTCAA CTCATGATAC AGACCCCTAA AGAAGAAGGT 
GCTAATGTCC TGACCACAGA AGCGCTCCTA CAACACCTGG ACTCGGCACT CCAGGCCAGC 
CGTGTCCATG TATACATGTA CAACAGGCAG TGGAAATTGG AACATTTGTG TTACAAATCA 
GGAGAGCTTA TCACAGAAAC AGGTTACATG GATCAGATAA TAGAATATCT TTACCCTTGT 
TTGATTATTA CACCTTTGGA CTGCTTCTGG GAAGGGGCGA AATTACAGTC TGGGACAGCA 
TACCTCCTAG GTAAACCTCC TTTGCGGTGG ACAAACTTCG ACCCTTTGGA ATTCCTGGAA 
GAGTTAAAGA AAATAAACTA TCAAGTGGAC AGCTGGGAGG AAATGCTGAA TAAGGCTGAG 
GTTGGTCATG GTTACATGGA CCGCCCCTGC CTCAATCCGG CCGATCCAGA CTGCCCCGCC 
ACAGCCCCCA ACAAAAATTC AACCAAACCT CTTGATATGG CCCTTGTTTT GAATGGTGGA 
TGTCATGGCT TATCCAGAAA GTATATGCAC TGGCAGGAGG AGTTGATTGT GGGTGGCACA 
GTCAAGAACA GCACTGGAAA ACTCGTCAGC GCCCATGCCC TGCAGACCAT GTTCCAGTTA 
ATGACTCCCA AGCAAATGTA CGAGCACTTC AAGGGGTACG AGTATGTCTC ACACATCAAC 
TGGAACGAGG ACAAAGCGGC AGCCATCCTG GAGGCCTGGC AGAGGACATA TGTGGAGGTG 
GTTCATCAGA GTGTCGCACA GAACTCCACT CAAAAGGTGC TTTCCTTCAC CACCACGACC 
CTGGACGACA TCCTGAAATC CTTCTCTGAC GTCAGTGTCA TCCGCGTGGC CAGCGGCTAC 
TTACTCATGC TCGCCTATGC CTGTCTAACC ATGCTGCGCT GGGACTGCTC CAAGTCCCAG 
GGTGCCGTGG GGCTGGCTGG CGTCCTGCTG GTTGCACTGT CAGTGGCTGC AGGACTGGGC 
CTGTGCTCAT TGATCGGAAT TTCCTTTAAC GCTGCAACAA CTCAGGTTTT GCCATTTCTC 
GCTCTTGGTG TTGGTGTGGA TGATGTTTTT CTTCTGGCCC ACGCCTTCAG TGAAACAGGA 
CAGAATAAAA GAATCCCTTT TGAGGACAGG ACCGGGGAGT GCCTGAAGCG CACAGGAGCC 
AGCGTGGCCC TCACGTCCAT CAGCAATGTC ACAGCCTTCT TCATGGCCGC GTTAATCCCA 
ATTCCCGCTC TGCGGGCGTT CTCCCTCCAG GCAGCGGTAG TAGTGGTGTT CAATTTTGCC 
ATGGTTCTGC TCATTTTTCC TGCAATTCTC AGCATGGATT TATATCGACG CGAGGACAGG 
AGACTGGATA TTTTCTGCTG TTTTACAAGC CCCTGCGTCA GCAGAGTGAT TCAGGTTGAA 
CCTCAGGCCT ACACCGACAC ACACGACAAT ACCCGCTACA GCCCCCCACC TCCCTACAGC 
AGCCACAGCT TTGCCCATGA AACGCAGATT ACCATGCAGT CCACTGTCCA GCTCCGCACG 
GAGTACGACC CCCACACGCA CGTGTACTAC ACCACCGCTG AGCCGCGCTC CGAGATCTCT 
GTGCAGCCCG TCACCGTGAC ACAGGACACC CTCAGCTGCC AGAGCCCAGA GAGCACCAGC 
TCCACAAGGG ACCTGCTCTC CCAGTTCTCC GACTCCAGCC TCCACTGCCT CGAGCCCCCC 
TGTACGAAGT GGACACTCTC ATCTTTTGCT GAGAAGCACT ATGCTCCTTT CCTCTTGAAA 
CCAAAAGCCA AGGTAGTGGT GATCTTCCTT TTTCTGGGCT TGCTGGGGGT CAGCCTTTAT 
GGCACCACCC GAGTGAGAGA CGGGCTGGAC CTTACGGACA TTGTACCTCG GGAAACCAGA 
GAATATGACT TTATTGCTGC ACAATTCAAA TACTTTTCTT TCTACAACAT GTATATAGTC 
ACCCAGAAAG CAGACTACCC GAATATCCAG CACTTACTTT ACGACCTACA CAGGAGTTTC 
AGTAACGTGA AGTATGTCAT GTTGGAAGAA AACAAACAGC TTCCCAAAAT GTGGCTGCAC 
TACTTCAGAG ACTGGCTTCA GGGACTTCAG GATGCATTTG ACAGTGACTG GGAAACCGGG 
AAAATCATGC CAAACAATTA CAAGAATGGA TCAGACGATG GAGTCCTTGC CTACAAACTC 
CTGGTGCAAA CCGGCAGCCG CGATAAGCCC ATCGACATCA GCCAGTTGAC TAAACAGCGT 
CTGGTGGATG CAGATGGCAT CATTAATCCC AGCGCTTTCT ACATCTACCT GACGGCTTGG 
GTCAGCAACG ACCCCGTCGC GTATGCTGCC TCCCAGGCCA ACATCCGGCC ACACCGACCA 
GAATGGGTCC ACGACAAAGC CGACTACATG CCTGAAACAA GGCTGAGAAT CCCGGCAGCA 
GAGCCCATCG AGTATGCCCA GTTCCCTTTC TACCTCAACG GCTTGCGGGA CACCTCAGAC 
TTTGTGGAGG CAATTGAAAA AGTAAGGACC ATCTGCAGCA ACTATACGAG CCTGGGGCTG 
TCCAGTTACC CCAACGGCTA CCCCTTCCTC TTCTGGGAGC AGTACATCGG CCTCCGCCAC 
TGGCTGCTGC TGTTCATCAG CGTGGTGTTG GCCTGCACAT TCCTCGTGTG CGCTGTCTTC 
CTTCTGAACC CCTGGACGGC CGGGATCATT GTGATGGTCC TGGCGCTGAT GACGGTCGAG 
CTGTTCGGCA TGATGGGCCT CATCGGAATC AAGCTCAGTG CCGTGCCCGT GGTCATCCTG 
ATCGCTTCTG TTGGCATAGG AGTGGAGTTC ACCGTTCACG TTGCTTTGGC CTTTCTGACG 
GCCATCGGCG ACAAGAACCG CAGGGCTGTG CTTGCCCTGG AGCACATGTT TGCACCCGTC 
CTGGATGGCG CCGTGTCCAC TCTGCTGGGA GTGCTGATGC TGGCGGGATC TGAGTTCGAC 
TTCATTGTCA GGTATTTCTT TGCTGTGCTG GCGATCCTCA CCATCCTCGG CGTTCTCAAT 
GGGCTGGTTT TGCTTCCCGT GCTTTTGTCT TTCTTTGGAC CATATCCTGA GGTGTCTCCA 
GCCAACGGCT TGAACCGCCT GCCCACACCC TCCCCTGAGC CACCCCCCAG CGTGGTCCGC 
TTCGCCATGC CGCCCGGCCA CACGCACAGC GGGTCTGATT CCTCCGACTC GGAGTATAGT 
TCCCAGACGA CAGTGTCAGG CCTCAGCGAG GAGCTTCGGC ACTACGAGGC CCAGCAGGGC 
GCGGGAGGCC CTGCCCACCA AGTGATCGTG GAAGCCACAG AAAACCCCGT CTTCGCCCAC 
TCCACTGTGG TCCATCCCGA ATCCAGGCAT CACCCACCCT CGAACCCGAG ACAGCAGCCC 
CACCTGGACT CAGGGTCCCT GCCTCCCGGA CGGCAAGGCC AGCAGCCCCG CAGGGACCCC 
CCCAGAGAAG GCTTGTGGCC ACCCCCCTAC AGACCGCGCA GAGACGCTTT TGAAATTTCT 
ACTGAAGGGC ATTCTGGCCC TAGCAATAGG GCCCGCTGGG GCCCTCGCGG GGCCCGTTCT 
CACAACCCTC GGAACCCAGC GTCCACTGCC ATGGGCAGCT CCGTGCCCGG CTACTGCCAG 
CCCATCACCA CTGTGACGGC TTCTGCCTCC GTGACTGTCG CCGTGCACCC GCCGCCTGTC 
CCTGGGCCTG GGCGGAACCC CCGAGGGGGA CTCTGCCCAG GCTACCCTGA GACTGACCAC 
GGCCTGTTTG AGGACCCCCA CGTGCCTTTC CACGTCCGGT GTGAGAGGAG GGATTCGAAG 
GTGGAAGTCA TTGAGCTGCA GGACGTGGAA TGCGAGGAGA GGCCCCGGGG AAGCAGCTCC 
AACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PTCH1 ( NM_001083602.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001077071.1 Homo sapiens patched 1 (PTCH1), transcript variant 1a, mRNA.

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CloneID	OHu19644
Clone ID Related Accession (Same CDS sequence)	NM_001083602.2 , NM_001083602.1
Accession Version	NM_001083602.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4146bp) Protein sequence SNP
Vector	pcDNA3.1⁺/C-(K)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-04-24
Organism	Homo sapiens(Human)
Product	protein patched homolog 1 isoform M
Comment	REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AB189436.1, U59464.1, AB209495.1, BX117041.1 and CR744004.1. This sequence is a reference standard in the RefSeqGene project. Transcript Variant: This variant (1a) differs in the 5' UTR and has multiple differences in the 5' coding region, compared to variant 1b. These differences result in a protein (isoform M) with a shorter N-terminus and distinct translation initiation site, compared to isoform L. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGGGAAGG CTACTGGCCG GAAAGCGCCG CTGTGGCTGA GAGCGAAGTT TCAGAGACTC 
TTATTTAAAC TGGGTTGTTA CATTCAAAAA AACTGCGGCA AGTTCTTGGT TGTGGGCCTC 
CTCATATTTG GGGCCTTCGC GGTGGGATTA AAAGCAGCGA ACCTCGAGAC CAACGTGGAG 
GAGCTGTGGG TGGAAGTTGG AGGACGAGTA AGTCGTGAAT TAAATTATAC TCGCCAGAAG 
ATTGGAGAAG AGGCTATGTT TAATCCTCAA CTCATGATAC AGACCCCTAA AGAAGAAGGT 
GCTAATGTCC TGACCACAGA AGCGCTCCTA CAACACCTGG ACTCGGCACT CCAGGCCAGC 
CGTGTCCATG TATACATGTA CAACAGGCAG TGGAAATTGG AACATTTGTG TTACAAATCA 
GGAGAGCTTA TCACAGAAAC AGGTTACATG GATCAGATAA TAGAATATCT TTACCCTTGT 
TTGATTATTA CACCTTTGGA CTGCTTCTGG GAAGGGGCGA AATTACAGTC TGGGACAGCA 
TACCTCCTAG GTAAACCTCC TTTGCGGTGG ACAAACTTCG ACCCTTTGGA ATTCCTGGAA 
GAGTTAAAGA AAATAAACTA TCAAGTGGAC AGCTGGGAGG AAATGCTGAA TAAGGCTGAG 
GTTGGTCATG GTTACATGGA CCGCCCCTGC CTCAATCCGG CCGATCCAGA CTGCCCCGCC 
ACAGCCCCCA ACAAAAATTC AACCAAACCT CTTGATATGG CCCTTGTTTT GAATGGTGGA 
TGTCATGGCT TATCCAGAAA GTATATGCAC TGGCAGGAGG AGTTGATTGT GGGTGGCACA 
GTCAAGAACA GCACTGGAAA ACTCGTCAGC GCCCATGCCC TGCAGACCAT GTTCCAGTTA 
ATGACTCCCA AGCAAATGTA CGAGCACTTC AAGGGGTACG AGTATGTCTC ACACATCAAC 
TGGAACGAGG ACAAAGCGGC AGCCATCCTG GAGGCCTGGC AGAGGACATA TGTGGAGGTG 
GTTCATCAGA GTGTCGCACA GAACTCCACT CAAAAGGTGC TTTCCTTCAC CACCACGACC 
CTGGACGACA TCCTGAAATC CTTCTCTGAC GTCAGTGTCA TCCGCGTGGC CAGCGGCTAC 
TTACTCATGC TCGCCTATGC CTGTCTAACC ATGCTGCGCT GGGACTGCTC CAAGTCCCAG 
GGTGCCGTGG GGCTGGCTGG CGTCCTGCTG GTTGCACTGT CAGTGGCTGC AGGACTGGGC 
CTGTGCTCAT TGATCGGAAT TTCCTTTAAC GCTGCAACAA CTCAGGTTTT GCCATTTCTC 
GCTCTTGGTG TTGGTGTGGA TGATGTTTTT CTTCTGGCCC ACGCCTTCAG TGAAACAGGA 
CAGAATAAAA GAATCCCTTT TGAGGACAGG ACCGGGGAGT GCCTGAAGCG CACAGGAGCC 
AGCGTGGCCC TCACGTCCAT CAGCAATGTC ACAGCCTTCT TCATGGCCGC GTTAATCCCA 
ATTCCCGCTC TGCGGGCGTT CTCCCTCCAG GCAGCGGTAG TAGTGGTGTT CAATTTTGCC 
ATGGTTCTGC TCATTTTTCC TGCAATTCTC AGCATGGATT TATATCGACG CGAGGACAGG 
AGACTGGATA TTTTCTGCTG TTTTACAAGC CCCTGCGTCA GCAGAGTGAT TCAGGTTGAA 
CCTCAGGCCT ACACCGACAC ACACGACAAT ACCCGCTACA GCCCCCCACC TCCCTACAGC 
AGCCACAGCT TTGCCCATGA AACGCAGATT ACCATGCAGT CCACTGTCCA GCTCCGCACG 
GAGTACGACC CCCACACGCA CGTGTACTAC ACCACCGCTG AGCCGCGCTC CGAGATCTCT 
GTGCAGCCCG TCACCGTGAC ACAGGACACC CTCAGCTGCC AGAGCCCAGA GAGCACCAGC 
TCCACAAGGG ACCTGCTCTC CCAGTTCTCC GACTCCAGCC TCCACTGCCT CGAGCCCCCC 
TGTACGAAGT GGACACTCTC ATCTTTTGCT GAGAAGCACT ATGCTCCTTT CCTCTTGAAA 
CCAAAAGCCA AGGTAGTGGT GATCTTCCTT TTTCTGGGCT TGCTGGGGGT CAGCCTTTAT 
GGCACCACCC GAGTGAGAGA CGGGCTGGAC CTTACGGACA TTGTACCTCG GGAAACCAGA 
GAATATGACT TTATTGCTGC ACAATTCAAA TACTTTTCTT TCTACAACAT GTATATAGTC 
ACCCAGAAAG CAGACTACCC GAATATCCAG CACTTACTTT ACGACCTACA CAGGAGTTTC 
AGTAACGTGA AGTATGTCAT GTTGGAAGAA AACAAACAGC TTCCCAAAAT GTGGCTGCAC 
TACTTCAGAG ACTGGCTTCA GGGACTTCAG GATGCATTTG ACAGTGACTG GGAAACCGGG 
AAAATCATGC CAAACAATTA CAAGAATGGA TCAGACGATG GAGTCCTTGC CTACAAACTC 
CTGGTGCAAA CCGGCAGCCG CGATAAGCCC ATCGACATCA GCCAGTTGAC TAAACAGCGT 
CTGGTGGATG CAGATGGCAT CATTAATCCC AGCGCTTTCT ACATCTACCT GACGGCTTGG 
GTCAGCAACG ACCCCGTCGC GTATGCTGCC TCCCAGGCCA ACATCCGGCC ACACCGACCA 
GAATGGGTCC ACGACAAAGC CGACTACATG CCTGAAACAA GGCTGAGAAT CCCGGCAGCA 
GAGCCCATCG AGTATGCCCA GTTCCCTTTC TACCTCAACG GCTTGCGGGA CACCTCAGAC 
TTTGTGGAGG CAATTGAAAA AGTAAGGACC ATCTGCAGCA ACTATACGAG CCTGGGGCTG 
TCCAGTTACC CCAACGGCTA CCCCTTCCTC TTCTGGGAGC AGTACATCGG CCTCCGCCAC 
TGGCTGCTGC TGTTCATCAG CGTGGTGTTG GCCTGCACAT TCCTCGTGTG CGCTGTCTTC 
CTTCTGAACC CCTGGACGGC CGGGATCATT GTGATGGTCC TGGCGCTGAT GACGGTCGAG 
CTGTTCGGCA TGATGGGCCT CATCGGAATC AAGCTCAGTG CCGTGCCCGT GGTCATCCTG 
ATCGCTTCTG TTGGCATAGG AGTGGAGTTC ACCGTTCACG TTGCTTTGGC CTTTCTGACG 
GCCATCGGCG ACAAGAACCG CAGGGCTGTG CTTGCCCTGG AGCACATGTT TGCACCCGTC 
CTGGATGGCG CCGTGTCCAC TCTGCTGGGA GTGCTGATGC TGGCGGGATC TGAGTTCGAC 
TTCATTGTCA GGTATTTCTT TGCTGTGCTG GCGATCCTCA CCATCCTCGG CGTTCTCAAT 
GGGCTGGTTT TGCTTCCCGT GCTTTTGTCT TTCTTTGGAC CATATCCTGA GGTGTCTCCA 
GCCAACGGCT TGAACCGCCT GCCCACACCC TCCCCTGAGC CACCCCCCAG CGTGGTCCGC 
TTCGCCATGC CGCCCGGCCA CACGCACAGC GGGTCTGATT CCTCCGACTC GGAGTATAGT 
TCCCAGACGA CAGTGTCAGG CCTCAGCGAG GAGCTTCGGC ACTACGAGGC CCAGCAGGGC 
GCGGGAGGCC CTGCCCACCA AGTGATCGTG GAAGCCACAG AAAACCCCGT CTTCGCCCAC 
TCCACTGTGG TCCATCCCGA ATCCAGGCAT CACCCACCCT CGAACCCGAG ACAGCAGCCC 
CACCTGGACT CAGGGTCCCT GCCTCCCGGA CGGCAAGGCC AGCAGCCCCG CAGGGACCCC 
CCCAGAGAAG GCTTGTGGCC ACCCCCCTAC AGACCGCGCA GAGACGCTTT TGAAATTTCT 
ACTGAAGGGC ATTCTGGCCC TAGCAATAGG GCCCGCTGGG GCCCTCGCGG GGCCCGTTCT 
CACAACCCTC GGAACCCAGC GTCCACTGCC ATGGGCAGCT CCGTGCCCGG CTACTGCCAG 
CCCATCACCA CTGTGACGGC TTCTGCCTCC GTGACTGTCG CCGTGCACCC GCCGCCTGTC 
CCTGGGCCTG GGCGGAACCC CCGAGGGGGA CTCTGCCCAG GCTACCCTGA GACTGACCAC 
GGCCTGTTTG AGGACCCCCA CGTGCCTTTC CACGTCCGGT GTGAGAGGAG GGATTCGAAG 
GTGGAAGTCA TTGAGCTGCA GGACGTGGAA TGCGAGGAGA GGCCCCGGGG AAGCAGCTCC 
AACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001077071.1
CDS	495..4640
Translation	MGKATGRKAPLWLRAKFQRLLFKLGCYIQKNCGKFLVVGLLIFGAFAVGLKAANLETNVEELWVEVGGRVSRELNYTRQKIGEEAMFNPQLMIQTPKEEGANVLTTEALLQHLDSALQASRVHVYMYNRQWKLEHLCYKSGELITETGYMDQIIEYLYPCLIITPLDCFWEGAKLQSGTAYLLGKPPLRWTNFDPLEFLEELKKINYQVDSWEEMLNKAEVGHGYMDRPCLNPADPDCPATAPNKNSTKPLDMALVLNGGCHGLSRKYMHWQEELIVGGTVKNSTGKLVSAHALQTMFQLMTPKQMYEHFKGYEYVSHINWNEDKAAAILEAWQRTYVEVVHQSVAQNSTQKVLSFTTTTLDDILKSFSDVSVIRVASGYLLMLAYACLTMLRWDCSKSQGAVGLAGVLLVALSVAAGLGLCSLIGISFNAATTQVLPFLALGVGVDDVFLLAHAFSETGQNKRIPFEDRTGECLKRTGASVALTSISNVTAFFMAALIPIPALRAFSLQAAVVVVFNFAMVLLIFPAILSMDLYRREDRRLDIFCCFTSPCVSRVIQVEPQAYTDTHDNTRYSPPPPYSSHSFAHETQITMQSTVQLRTEYDPHTHVYYTTAEPRSEISVQPVTVTQDTLSCQSPESTSSTRDLLSQFSDSSLHCLEPPCTKWTLSSFAEKHYAPFLLKPKAKVVVIFLFLGLLGVSLYGTTRVRDGLDLTDIVPRETREYDFIAAQFKYFSFYNMYIVTQKADYPNIQHLLYDLHRSFSNVKYVMLEENKQLPKMWLHYFRDWLQGLQDAFDSDWETGKIMPNNYKNGSDDGVLAYKLLVQTGSRDKPIDISQLTKQRLVDADGIINPSAFYIYLTAWVSNDPVAYAASQANIRPHRPEWVHDKADYMPETRLRIPAAEPIEYAQFPFYLNGLRDTSDFVEAIEKVRTICSNYTSLGLSSYPNGYPFLFWEQYIGLRHWLLLFISVVLACTFLVCAVFLLNPWTAGIIVMVLALMTVELFGMMGLIGIKLSAVPVVILIASVGIGVEFTVHVALAFLTAIGDKNRRAVLALEHMFAPVLDGAVSTLLGVLMLAGSEFDFIVRYFFAVLAILTILGVLNGLVLLPVLLSFFGPYPEVSPANGLNRLPTPSPEPPPSVVRFAMPPGHTHSGSDSSDSEYSSQTTVSGLSEELRHYEAQQGAGGPAHQVIVEATENPVFAHSTVVHPESRHHPPSNPRQQPHLDSGSLPPGRQGQQPRRDPPREGLWPPPYRPRRDAFEISTEGHSGPSNRARWGPRGARSHNPRNPASTAMGSSVPGYCQPITTVTASASVTVAVHPPPVPGPGRNPRGGLCPGYPETDHGLFEDPHVPFHVRCERRDSKVEVIELQDVECEERPRGSSSN

Target ORF information:

RefSeq Version	NM_001083602.1
Organism	Homo sapiens(Human)
Definition	Homo sapiens patched 1 (PTCH1), transcript variant 1a, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001083602.1

ORF Insert Sequence:

ATGGGGAAGG CTACTGGCCG GAAAGCGCCG CTGTGGCTGA GAGCGAAGTT TCAGAGACTC 
TTATTTAAAC TGGGTTGTTA CATTCAAAAA AACTGCGGCA AGTTCTTGGT TGTGGGCCTC 
CTCATATTTG GGGCCTTCGC GGTGGGATTA AAAGCAGCGA ACCTCGAGAC CAACGTGGAG 
GAGCTGTGGG TGGAAGTTGG AGGACGAGTA AGTCGTGAAT TAAATTATAC TCGCCAGAAG 
ATTGGAGAAG AGGCTATGTT TAATCCTCAA CTCATGATAC AGACCCCTAA AGAAGAAGGT 
GCTAATGTCC TGACCACAGA AGCGCTCCTA CAACACCTGG ACTCGGCACT CCAGGCCAGC 
CGTGTCCATG TATACATGTA CAACAGGCAG TGGAAATTGG AACATTTGTG TTACAAATCA 
GGAGAGCTTA TCACAGAAAC AGGTTACATG GATCAGATAA TAGAATATCT TTACCCTTGT 
TTGATTATTA CACCTTTGGA CTGCTTCTGG GAAGGGGCGA AATTACAGTC TGGGACAGCA 
TACCTCCTAG GTAAACCTCC TTTGCGGTGG ACAAACTTCG ACCCTTTGGA ATTCCTGGAA 
GAGTTAAAGA AAATAAACTA TCAAGTGGAC AGCTGGGAGG AAATGCTGAA TAAGGCTGAG 
GTTGGTCATG GTTACATGGA CCGCCCCTGC CTCAATCCGG CCGATCCAGA CTGCCCCGCC 
ACAGCCCCCA ACAAAAATTC AACCAAACCT CTTGATATGG CCCTTGTTTT GAATGGTGGA 
TGTCATGGCT TATCCAGAAA GTATATGCAC TGGCAGGAGG AGTTGATTGT GGGTGGCACA 
GTCAAGAACA GCACTGGAAA ACTCGTCAGC GCCCATGCCC TGCAGACCAT GTTCCAGTTA 
ATGACTCCCA AGCAAATGTA CGAGCACTTC AAGGGGTACG AGTATGTCTC ACACATCAAC 
TGGAACGAGG ACAAAGCGGC AGCCATCCTG GAGGCCTGGC AGAGGACATA TGTGGAGGTG 
GTTCATCAGA GTGTCGCACA GAACTCCACT CAAAAGGTGC TTTCCTTCAC CACCACGACC 
CTGGACGACA TCCTGAAATC CTTCTCTGAC GTCAGTGTCA TCCGCGTGGC CAGCGGCTAC 
TTACTCATGC TCGCCTATGC CTGTCTAACC ATGCTGCGCT GGGACTGCTC CAAGTCCCAG 
GGTGCCGTGG GGCTGGCTGG CGTCCTGCTG GTTGCACTGT CAGTGGCTGC AGGACTGGGC 
CTGTGCTCAT TGATCGGAAT TTCCTTTAAC GCTGCAACAA CTCAGGTTTT GCCATTTCTC 
GCTCTTGGTG TTGGTGTGGA TGATGTTTTT CTTCTGGCCC ACGCCTTCAG TGAAACAGGA 
CAGAATAAAA GAATCCCTTT TGAGGACAGG ACCGGGGAGT GCCTGAAGCG CACAGGAGCC 
AGCGTGGCCC TCACGTCCAT CAGCAATGTC ACAGCCTTCT TCATGGCCGC GTTAATCCCA 
ATTCCCGCTC TGCGGGCGTT CTCCCTCCAG GCAGCGGTAG TAGTGGTGTT CAATTTTGCC 
ATGGTTCTGC TCATTTTTCC TGCAATTCTC AGCATGGATT TATATCGACG CGAGGACAGG 
AGACTGGATA TTTTCTGCTG TTTTACAAGC CCCTGCGTCA GCAGAGTGAT TCAGGTTGAA 
CCTCAGGCCT ACACCGACAC ACACGACAAT ACCCGCTACA GCCCCCCACC TCCCTACAGC 
AGCCACAGCT TTGCCCATGA AACGCAGATT ACCATGCAGT CCACTGTCCA GCTCCGCACG 
GAGTACGACC CCCACACGCA CGTGTACTAC ACCACCGCTG AGCCGCGCTC CGAGATCTCT 
GTGCAGCCCG TCACCGTGAC ACAGGACACC CTCAGCTGCC AGAGCCCAGA GAGCACCAGC 
TCCACAAGGG ACCTGCTCTC CCAGTTCTCC GACTCCAGCC TCCACTGCCT CGAGCCCCCC 
TGTACGAAGT GGACACTCTC ATCTTTTGCT GAGAAGCACT ATGCTCCTTT CCTCTTGAAA 
CCAAAAGCCA AGGTAGTGGT GATCTTCCTT TTTCTGGGCT TGCTGGGGGT CAGCCTTTAT 
GGCACCACCC GAGTGAGAGA CGGGCTGGAC CTTACGGACA TTGTACCTCG GGAAACCAGA 
GAATATGACT TTATTGCTGC ACAATTCAAA TACTTTTCTT TCTACAACAT GTATATAGTC 
ACCCAGAAAG CAGACTACCC GAATATCCAG CACTTACTTT ACGACCTACA CAGGAGTTTC 
AGTAACGTGA AGTATGTCAT GTTGGAAGAA AACAAACAGC TTCCCAAAAT GTGGCTGCAC 
TACTTCAGAG ACTGGCTTCA GGGACTTCAG GATGCATTTG ACAGTGACTG GGAAACCGGG 
AAAATCATGC CAAACAATTA CAAGAATGGA TCAGACGATG GAGTCCTTGC CTACAAACTC 
CTGGTGCAAA CCGGCAGCCG CGATAAGCCC ATCGACATCA GCCAGTTGAC TAAACAGCGT 
CTGGTGGATG CAGATGGCAT CATTAATCCC AGCGCTTTCT ACATCTACCT GACGGCTTGG 
GTCAGCAACG ACCCCGTCGC GTATGCTGCC TCCCAGGCCA ACATCCGGCC ACACCGACCA 
GAATGGGTCC ACGACAAAGC CGACTACATG CCTGAAACAA GGCTGAGAAT CCCGGCAGCA 
GAGCCCATCG AGTATGCCCA GTTCCCTTTC TACCTCAACG GCTTGCGGGA CACCTCAGAC 
TTTGTGGAGG CAATTGAAAA AGTAAGGACC ATCTGCAGCA ACTATACGAG CCTGGGGCTG 
TCCAGTTACC CCAACGGCTA CCCCTTCCTC TTCTGGGAGC AGTACATCGG CCTCCGCCAC 
TGGCTGCTGC TGTTCATCAG CGTGGTGTTG GCCTGCACAT TCCTCGTGTG CGCTGTCTTC 
CTTCTGAACC CCTGGACGGC CGGGATCATT GTGATGGTCC TGGCGCTGAT GACGGTCGAG 
CTGTTCGGCA TGATGGGCCT CATCGGAATC AAGCTCAGTG CCGTGCCCGT GGTCATCCTG 
ATCGCTTCTG TTGGCATAGG AGTGGAGTTC ACCGTTCACG TTGCTTTGGC CTTTCTGACG 
GCCATCGGCG ACAAGAACCG CAGGGCTGTG CTTGCCCTGG AGCACATGTT TGCACCCGTC 
CTGGATGGCG CCGTGTCCAC TCTGCTGGGA GTGCTGATGC TGGCGGGATC TGAGTTCGAC 
TTCATTGTCA GGTATTTCTT TGCTGTGCTG GCGATCCTCA CCATCCTCGG CGTTCTCAAT 
GGGCTGGTTT TGCTTCCCGT GCTTTTGTCT TTCTTTGGAC CATATCCTGA GGTGTCTCCA 
GCCAACGGCT TGAACCGCCT GCCCACACCC TCCCCTGAGC CACCCCCCAG CGTGGTCCGC 
TTCGCCATGC CGCCCGGCCA CACGCACAGC GGGTCTGATT CCTCCGACTC GGAGTATAGT 
TCCCAGACGA CAGTGTCAGG CCTCAGCGAG GAGCTTCGGC ACTACGAGGC CCAGCAGGGC 
GCGGGAGGCC CTGCCCACCA AGTGATCGTG GAAGCCACAG AAAACCCCGT CTTCGCCCAC 
TCCACTGTGG TCCATCCCGA ATCCAGGCAT CACCCACCCT CGAACCCGAG ACAGCAGCCC 
CACCTGGACT CAGGGTCCCT GCCTCCCGGA CGGCAAGGCC AGCAGCCCCG CAGGGACCCC 
CCCAGAGAAG GCTTGTGGCC ACCCCCCTAC AGACCGCGCA GAGACGCTTT TGAAATTTCT 
ACTGAAGGGC ATTCTGGCCC TAGCAATAGG GCCCGCTGGG GCCCTCGCGG GGCCCGTTCT 
CACAACCCTC GGAACCCAGC GTCCACTGCC ATGGGCAGCT CCGTGCCCGG CTACTGCCAG 
CCCATCACCA CTGTGACGGC TTCTGCCTCC GTGACTGTCG CCGTGCACCC GCCGCCTGTC 
CCTGGGCCTG GGCGGAACCC CCGAGGGGGA CTCTGCCCAG GCTACCCTGA GACTGACCAC 
GGCCTGTTTG AGGACCCCCA CGTGCCTTTC CACGTCCGGT GTGAGAGGAG GGATTCGAAG 
GTGGAAGTCA TTGAGCTGCA GGACGTGGAA TGCGAGGAGA GGCCCCGGGG AAGCAGCTCC 
AACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PTCH1 ( NM_001354918.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001341847.1 Homo sapiens patched 1 (PTCH1), transcript variant 8, mRNA.

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CloneID	OHu59996
Clone ID Related Accession (Same CDS sequence)	NM_001354918.1 , NM_001354918.2
Accession Version	NM_001354918.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4188bp) Protein sequence SNP
Vector	pcDNA3.1⁺/C-(K)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-06-10
Organism	Homo sapiens(human)
Product	protein patched homolog 1 isoform 8
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AI494319.1, KY652975.1, AB209495.1, AK124593.1, CR744004.1 and AI417736.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## CDS exon combination :: KY652975.1 [ECO:0000331] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## inferred exon combination :: based on alignments, homology ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGGCCTCGG CTGGTAACGC CGCCGAGCCC CAGGACCGCG GCGGCGGCGG CAGCGGCTGT 
ATCGGTGCCC CGGGACGGCC GGCTGGAGGC GGGAGGCGCA GACGGACGGG GGGGCTGCGC 
CGTGCTGCCG CGCCGGACCG GGACTATCTG CACCGGCCCA GCTACTGCGA CGCCGCCTTC 
GCTCTGGAGC AGATTTCCAA GGGGAAGGCT ACTGGCCGGA AAGCGCCGCT GTGGCTGAGA 
GCGAAGTTTC AGAGACTCTT ATTTAAACTG GGTTGTTACA TTCAAAAAAA CTGCGGCAAG 
TTCTTGGTTG TGGGCCTCCT CATATTTGGG GCCTTCGCGG TGGGATTAAA AGCAGCGAAC 
CTCGAGACCA ACGTGGAGGA GCTGTGGGTG GAAGTTGGAG GACGAGTAAG TCGTGAATTA 
AATTATACTC GCCAGAAGAT TGGAGAAGAG GCTATGTTTA ATCCTCAACT CATGATACAG 
ACCCCTAAAG AAGAAGGTGC TAATGTCCTG ACCACAGAAG CGCTCCTACA ACACCTGGAC 
TCGGCACTCC AGGCCAGCCG TGTCCATGTA TACATGTACA ACAGGCAGTG GAAATTGGAA 
CATTTGTGTT ACAAATCAGG AGAGCTTATC ACAGAAACAG GTTACATGGA TCAGATAATA 
GAATATCTTT ACCCTTGTTT GATTATTACA CCTTTGGACT GCTTCTGGGA AGGGGCGAAA 
TTACAGTCTG GGACAGCATA CCTCCTAGGT AAACCTCCTT TGCGGTGGAC AAACTTCGAC 
CCTTTGGAAT TCCTGGAAGA GTTAAAGAAA ATAAACTATC AAGTGGACAG CTGGGAGGAA 
ATGCTGAATA AGGCTGAGGT TGGTCATGGT TACATGGACC GCCCCTGCCT CAATCCGGCC 
GATCCAGACT GCCCCGCCAC AGCCCCCAAC AAAAATTCAA CCAAACCTCT TGATATGGCC 
CTTGTTTTGA ATGGTGGATG TCATGGCTTA TCCAGAAAGT ATATGCACTG GCAGGAGGAG 
TTGATTGTGG GTGGCACAGT CAAGAACAGC ACTGGAAAAC TCGTCAGCGC CCATGCCCTG 
CAGACCATGT TCCAGTTAAT GACTCCCAAG CAAATGTACG AGCACTTCAA GGGGTACGAG 
TATGTCTCAC ACATCAACTG GAACGAGGAC AAAGCGGCAG CCATCCTGGA GGCCTGGCAG 
AGGACATATG TGGAGGTGGT TCATCAGAGT GTCGCACAGA ACTCCACTCA AAAGGTGCTT 
TCCTTCACCA CCACGACCCT GGACGACATC CTGAAATCCT TCTCTGACGT CAGTGTCATC 
CGCGTGGCCA GCGGCTACTT ACTCATGGTT TTGCCATTTC TCGCTCTTGG TGTTGGTGTG 
GATGATGTTT TTCTTCTGGC CCACGCCTTC AGTGAAACAG GACAGAATAA AAGAATCCCT 
TTTGAGGACA GGACCGGGGA GTGCCTGAAG CGCACAGGAG CCAGCGTGGC CCTCACGTCC 
ATCAGCAATG TCACAGCCTT CTTCATGGCC GCGTTAATCC CAATTCCCGC TCTGCGGGCG 
TTCTCCCTCC AGGCAGCGGT AGTAGTGGTG TTCAATTTTG CCATGGTTCT GCTCATTTTT 
CCTGCAATTC TCAGCATGGA TTTATATCGA CGCGAGGACA GGAGACTGGA TATTTTCTGC 
TGTTTTACAA GCCCCTGCGT CAGCAGAGTG ATTCAGGTTG AACCTCAGGC CTACACCGAC 
ACACACGACA ATACCCGCTA CAGCCCCCCA CCTCCCTACA GCAGCCACAG CTTTGCCCAT 
GAAACGCAGA TTACCATGCA GTCCACTGTC CAGCTCCGCA CGGAGTACGA CCCCCACACG 
CACGTGTACT ACACCACCGC TGAGCCGCGC TCCGAGATCT CTGTGCAGCC CGTCACCGTG 
ACACAGGACA CCCTCAGCTG CCAGAGCCCA GAGAGCACCA GCTCCACAAG GGACCTGCTC 
TCCCAGTTCT CCGACTCCAG CCTCCACTGC CTCGAGCCCC CCTGTACGAA GTGGACACTC 
TCATCTTTTG CTGAGAAGCA CTATGCTCCT TTCCTCTTGA AACCAAAAGC CAAGGTAGTG 
GTGATCTTCC TTTTTCTGGG CTTGCTGGGG GTCAGCCTTT ATGGCACCAC CCGAGTGAGA 
GACGGGCTGG ACCTTACGGA CATTGTACCT CGGGAAACCA GAGAATATGA CTTTATTGCT 
GCACAATTCA AATACTTTTC TTTCTACAAC ATGTATATAG TCACCCAGAA AGCAGACTAC 
CCGAATATCC AGCACTTACT TTACGACCTA CACAGGAGTT TCAGTAACGT GAAGTATGTC 
ATGTTGGAAG AAAACAAACA GCTTCCCAAA ATGTGGCTGC ACTACTTCAG AGACTGGCTT 
CAGGGACTTC AGGATGCATT TGACAGTGAC TGGGAAACCG GGAAAATCAT GCCAAACAAT 
TACAAGAATG GATCAGACGA TGGAGTCCTT GCCTACAAAC TCCTGGTGCA AACCGGCAGC 
CGCGATAAGC CCATCGACAT CAGCCAGTTG ACTAAACAGC GTCTGGTGGA TGCAGATGGC 
ATCATTAATC CCAGCGCTTT CTACATCTAC CTGACGGCTT GGGTCAGCAA CGACCCCGTC 
GCGTATGCTG CCTCCCAGGC CAACATCCGG CCACACCGAC CAGAATGGGT CCACGACAAA 
GCCGACTACA TGCCTGAAAC AAGGCTGAGA ATCCCGGCAG CAGAGCCCAT CGAGTATGCC 
CAGTTCCCTT TCTACCTCAA CGGCTTGCGG GACACCTCAG ACTTTGTGGA GGCAATTGAA 
AAAGTAAGGA CCATCTGCAG CAACTATACG AGCCTGGGGC TGTCCAGTTA CCCCAACGGC 
TACCCCTTCC TCTTCTGGGA GCAGTACATC GGCCTCCGCC ACTGGCTGCT GCTGTTCATC 
AGCGTGGTGT TGGCCTGCAC ATTCCTCGTG TGCGCTGTCT TCCTTCTGAA CCCCTGGACG 
GCCGGGATCA TTGTGATGGT CCTGGCGCTG ATGACGGTCG AGCTGTTCGG CATGATGGGC 
CTCATCGGAA TCAAGCTCAG TGCCGTGCCC GTGGTCATCC TGATCGCTTC TGTTGGCATA 
GGAGTGGAGT TCACCGTTCA CGTTGCTTTG GCCTTTCTGA CGGCCATCGG CGACAAGAAC 
CGCAGGGCTG TGCTTGCCCT GGAGCACATG TTTGCACCCG TCCTGGATGG CGCCGTGTCC 
ACTCTGCTGG GAGTGCTGAT GCTGGCGGGA TCTGAGTTCG ACTTCATTGT CAGGTATTTC 
TTTGCTGTGC TGGCGATCCT CACCATCCTC GGCGTTCTCA ATGGGCTGGT TTTGCTTCCC 
GTGCTTTTGT CTTTCTTTGG ACCATATCCT GAGGTGTCTC CAGCCAACGG CTTGAACCGC 
CTGCCCACAC CCTCCCCTGA GCCACCCCCC AGCGTGGTCC GCTTCGCCAT GCCGCCCGGC 
CACACGCACA GCGGGTCTGA TTCCTCCGAC TCGGAGTATA GTTCCCAGAC GACAGTGTCA 
GGCCTCAGCG AGGAGCTTCG GCACTACGAG GCCCAGCAGG GCGCGGGAGG CCCTGCCCAC 
CAAGTGATCG TGGAAGCCAC AGAAAACCCC GTCTTCGCCC ACTCCACTGT GGTCCATCCC 
GAATCCAGGC ATCACCCACC CTCGAACCCG AGACAGCAGC CCCACCTGGA CTCAGGGTCC 
CTGCCTCCCG GACGGCAAGG CCAGCAGCCC CGCAGGGACC CCCCCAGAGA AGGCTTGTGG 
CCACCCCCCT ACAGACCGCG CAGAGACGCT TTTGAAATTT CTACTGAAGG GCATTCTGGC 
CCTAGCAATA GGGCCCGCTG GGGCCCTCGC GGGGCCCGTT CTCACAACCC TCGGAACCCA 
GCGTCCACTG CCATGGGCAG CTCCGTGCCC GGCTACTGCC AGCCCATCAC CACTGTGACG 
GCTTCTGCCT CCGTGACTGT CGCCGTGCAC CCGCCGCCTG TCCCTGGGCC TGGGCGGAAC 
CCCCGAGGGG GACTCTGCCC AGGCTACCCT GAGACTGACC ACGGCCTGTT TGAGGACCCC 
CACGTGCCTT TCCACGTCCG GTGTGAGAGG AGGGATTCGA AGGTGGAAGT CATTGAGCTG 
CAGGACGTGG AATGCGAGGA GAGGCCCCGG GGAAGCAGCT CCAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001341847.1
CDS	189..4376
Translation	MASAGNAAEPQDRGGGGSGCIGAPGRPAGGGRRRRTGGLRRAAAPDRDYLHRPSYCDAAFALEQISKGKATGRKAPLWLRAKFQRLLFKLGCYIQKNCGKFLVVGLLIFGAFAVGLKAANLETNVEELWVEVGGRVSRELNYTRQKIGEEAMFNPQLMIQTPKEEGANVLTTEALLQHLDSALQASRVHVYMYNRQWKLEHLCYKSGELITETGYMDQIIEYLYPCLIITPLDCFWEGAKLQSGTAYLLGKPPLRWTNFDPLEFLEELKKINYQVDSWEEMLNKAEVGHGYMDRPCLNPADPDCPATAPNKNSTKPLDMALVLNGGCHGLSRKYMHWQEELIVGGTVKNSTGKLVSAHALQTMFQLMTPKQMYEHFKGYEYVSHINWNEDKAAAILEAWQRTYVEVVHQSVAQNSTQKVLSFTTTTLDDILKSFSDVSVIRVASGYLLMVLPFLALGVGVDDVFLLAHAFSETGQNKRIPFEDRTGECLKRTGASVALTSISNVTAFFMAALIPIPALRAFSLQAAVVVVFNFAMVLLIFPAILSMDLYRREDRRLDIFCCFTSPCVSRVIQVEPQAYTDTHDNTRYSPPPPYSSHSFAHETQITMQSTVQLRTEYDPHTHVYYTTAEPRSEISVQPVTVTQDTLSCQSPESTSSTRDLLSQFSDSSLHCLEPPCTKWTLSSFAEKHYAPFLLKPKAKVVVIFLFLGLLGVSLYGTTRVRDGLDLTDIVPRETREYDFIAAQFKYFSFYNMYIVTQKADYPNIQHLLYDLHRSFSNVKYVMLEENKQLPKMWLHYFRDWLQGLQDAFDSDWETGKIMPNNYKNGSDDGVLAYKLLVQTGSRDKPIDISQLTKQRLVDADGIINPSAFYIYLTAWVSNDPVAYAASQANIRPHRPEWVHDKADYMPETRLRIPAAEPIEYAQFPFYLNGLRDTSDFVEAIEKVRTICSNYTSLGLSSYPNGYPFLFWEQYIGLRHWLLLFISVVLACTFLVCAVFLLNPWTAGIIVMVLALMTVELFGMMGLIGIKLSAVPVVILIASVGIGVEFTVHVALAFLTAIGDKNRRAVLALEHMFAPVLDGAVSTLLGVLMLAGSEFDFIVRYFFAVLAILTILGVLNGLVLLPVLLSFFGPYPEVSPANGLNRLPTPSPEPPPSVVRFAMPPGHTHSGSDSSDSEYSSQTTVSGLSEELRHYEAQQGAGGPAHQVIVEATENPVFAHSTVVHPESRHHPPSNPRQQPHLDSGSLPPGRQGQQPRRDPPREGLWPPPYRPRRDAFEISTEGHSGPSNRARWGPRGARSHNPRNPASTAMGSSVPGYCQPITTVTASASVTVAVHPPPVPGPGRNPRGGLCPGYPETDHGLFEDPHVPFHVRCERRDSKVEVIELQDVECEERPRGSSSN

Target ORF information:

RefSeq Version	NM_001354918.1
Organism	Homo sapiens(human)
Definition	Homo sapiens patched 1 (PTCH1), transcript variant 8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001354918.1

ORF Insert Sequence:

ATGGCCTCGG CTGGTAACGC CGCCGAGCCC CAGGACCGCG GCGGCGGCGG CAGCGGCTGT 
ATCGGTGCCC CGGGACGGCC GGCTGGAGGC GGGAGGCGCA GACGGACGGG GGGGCTGCGC 
CGTGCTGCCG CGCCGGACCG GGACTATCTG CACCGGCCCA GCTACTGCGA CGCCGCCTTC 
GCTCTGGAGC AGATTTCCAA GGGGAAGGCT ACTGGCCGGA AAGCGCCGCT GTGGCTGAGA 
GCGAAGTTTC AGAGACTCTT ATTTAAACTG GGTTGTTACA TTCAAAAAAA CTGCGGCAAG 
TTCTTGGTTG TGGGCCTCCT CATATTTGGG GCCTTCGCGG TGGGATTAAA AGCAGCGAAC 
CTCGAGACCA ACGTGGAGGA GCTGTGGGTG GAAGTTGGAG GACGAGTAAG TCGTGAATTA 
AATTATACTC GCCAGAAGAT TGGAGAAGAG GCTATGTTTA ATCCTCAACT CATGATACAG 
ACCCCTAAAG AAGAAGGTGC TAATGTCCTG ACCACAGAAG CGCTCCTACA ACACCTGGAC 
TCGGCACTCC AGGCCAGCCG TGTCCATGTA TACATGTACA ACAGGCAGTG GAAATTGGAA 
CATTTGTGTT ACAAATCAGG AGAGCTTATC ACAGAAACAG GTTACATGGA TCAGATAATA 
GAATATCTTT ACCCTTGTTT GATTATTACA CCTTTGGACT GCTTCTGGGA AGGGGCGAAA 
TTACAGTCTG GGACAGCATA CCTCCTAGGT AAACCTCCTT TGCGGTGGAC AAACTTCGAC 
CCTTTGGAAT TCCTGGAAGA GTTAAAGAAA ATAAACTATC AAGTGGACAG CTGGGAGGAA 
ATGCTGAATA AGGCTGAGGT TGGTCATGGT TACATGGACC GCCCCTGCCT CAATCCGGCC 
GATCCAGACT GCCCCGCCAC AGCCCCCAAC AAAAATTCAA CCAAACCTCT TGATATGGCC 
CTTGTTTTGA ATGGTGGATG TCATGGCTTA TCCAGAAAGT ATATGCACTG GCAGGAGGAG 
TTGATTGTGG GTGGCACAGT CAAGAACAGC ACTGGAAAAC TCGTCAGCGC CCATGCCCTG 
CAGACCATGT TCCAGTTAAT GACTCCCAAG CAAATGTACG AGCACTTCAA GGGGTACGAG 
TATGTCTCAC ACATCAACTG GAACGAGGAC AAAGCGGCAG CCATCCTGGA GGCCTGGCAG 
AGGACATATG TGGAGGTGGT TCATCAGAGT GTCGCACAGA ACTCCACTCA AAAGGTGCTT 
TCCTTCACCA CCACGACCCT GGACGACATC CTGAAATCCT TCTCTGACGT CAGTGTCATC 
CGCGTGGCCA GCGGCTACTT ACTCATGGTT TTGCCATTTC TCGCTCTTGG TGTTGGTGTG 
GATGATGTTT TTCTTCTGGC CCACGCCTTC AGTGAAACAG GACAGAATAA AAGAATCCCT 
TTTGAGGACA GGACCGGGGA GTGCCTGAAG CGCACAGGAG CCAGCGTGGC CCTCACGTCC 
ATCAGCAATG TCACAGCCTT CTTCATGGCC GCGTTAATCC CAATTCCCGC TCTGCGGGCG 
TTCTCCCTCC AGGCAGCGGT AGTAGTGGTG TTCAATTTTG CCATGGTTCT GCTCATTTTT 
CCTGCAATTC TCAGCATGGA TTTATATCGA CGCGAGGACA GGAGACTGGA TATTTTCTGC 
TGTTTTACAA GCCCCTGCGT CAGCAGAGTG ATTCAGGTTG AACCTCAGGC CTACACCGAC 
ACACACGACA ATACCCGCTA CAGCCCCCCA CCTCCCTACA GCAGCCACAG CTTTGCCCAT 
GAAACGCAGA TTACCATGCA GTCCACTGTC CAGCTCCGCA CGGAGTACGA CCCCCACACG 
CACGTGTACT ACACCACCGC TGAGCCGCGC TCCGAGATCT CTGTGCAGCC CGTCACCGTG 
ACACAGGACA CCCTCAGCTG CCAGAGCCCA GAGAGCACCA GCTCCACAAG GGACCTGCTC 
TCCCAGTTCT CCGACTCCAG CCTCCACTGC CTCGAGCCCC CCTGTACGAA GTGGACACTC 
TCATCTTTTG CTGAGAAGCA CTATGCTCCT TTCCTCTTGA AACCAAAAGC CAAGGTAGTG 
GTGATCTTCC TTTTTCTGGG CTTGCTGGGG GTCAGCCTTT ATGGCACCAC CCGAGTGAGA 
GACGGGCTGG ACCTTACGGA CATTGTACCT CGGGAAACCA GAGAATATGA CTTTATTGCT 
GCACAATTCA AATACTTTTC TTTCTACAAC ATGTATATAG TCACCCAGAA AGCAGACTAC 
CCGAATATCC AGCACTTACT TTACGACCTA CACAGGAGTT TCAGTAACGT GAAGTATGTC 
ATGTTGGAAG AAAACAAACA GCTTCCCAAA ATGTGGCTGC ACTACTTCAG AGACTGGCTT 
CAGGGACTTC AGGATGCATT TGACAGTGAC TGGGAAACCG GGAAAATCAT GCCAAACAAT 
TACAAGAATG GATCAGACGA TGGAGTCCTT GCCTACAAAC TCCTGGTGCA AACCGGCAGC 
CGCGATAAGC CCATCGACAT CAGCCAGTTG ACTAAACAGC GTCTGGTGGA TGCAGATGGC 
ATCATTAATC CCAGCGCTTT CTACATCTAC CTGACGGCTT GGGTCAGCAA CGACCCCGTC 
GCGTATGCTG CCTCCCAGGC CAACATCCGG CCACACCGAC CAGAATGGGT CCACGACAAA 
GCCGACTACA TGCCTGAAAC AAGGCTGAGA ATCCCGGCAG CAGAGCCCAT CGAGTATGCC 
CAGTTCCCTT TCTACCTCAA CGGCTTGCGG GACACCTCAG ACTTTGTGGA GGCAATTGAA 
AAAGTAAGGA CCATCTGCAG CAACTATACG AGCCTGGGGC TGTCCAGTTA CCCCAACGGC 
TACCCCTTCC TCTTCTGGGA GCAGTACATC GGCCTCCGCC ACTGGCTGCT GCTGTTCATC 
AGCGTGGTGT TGGCCTGCAC ATTCCTCGTG TGCGCTGTCT TCCTTCTGAA CCCCTGGACG 
GCCGGGATCA TTGTGATGGT CCTGGCGCTG ATGACGGTCG AGCTGTTCGG CATGATGGGC 
CTCATCGGAA TCAAGCTCAG TGCCGTGCCC GTGGTCATCC TGATCGCTTC TGTTGGCATA 
GGAGTGGAGT TCACCGTTCA CGTTGCTTTG GCCTTTCTGA CGGCCATCGG CGACAAGAAC 
CGCAGGGCTG TGCTTGCCCT GGAGCACATG TTTGCACCCG TCCTGGATGG CGCCGTGTCC 
ACTCTGCTGG GAGTGCTGAT GCTGGCGGGA TCTGAGTTCG ACTTCATTGT CAGGTATTTC 
TTTGCTGTGC TGGCGATCCT CACCATCCTC GGCGTTCTCA ATGGGCTGGT TTTGCTTCCC 
GTGCTTTTGT CTTTCTTTGG ACCATATCCT GAGGTGTCTC CAGCCAACGG CTTGAACCGC 
CTGCCCACAC CCTCCCCTGA GCCACCCCCC AGCGTGGTCC GCTTCGCCAT GCCGCCCGGC 
CACACGCACA GCGGGTCTGA TTCCTCCGAC TCGGAGTATA GTTCCCAGAC GACAGTGTCA 
GGCCTCAGCG AGGAGCTTCG GCACTACGAG GCCCAGCAGG GCGCGGGAGG CCCTGCCCAC 
CAAGTGATCG TGGAAGCCAC AGAAAACCCC GTCTTCGCCC ACTCCACTGT GGTCCATCCC 
GAATCCAGGC ATCACCCACC CTCGAACCCG AGACAGCAGC CCCACCTGGA CTCAGGGTCC 
CTGCCTCCCG GACGGCAAGG CCAGCAGCCC CGCAGGGACC CCCCCAGAGA AGGCTTGTGG 
CCACCCCCCT ACAGACCGCG CAGAGACGCT TTTGAAATTT CTACTGAAGG GCATTCTGGC 
CCTAGCAATA GGGCCCGCTG GGGCCCTCGC GGGGCCCGTT CTCACAACCC TCGGAACCCA 
GCGTCCACTG CCATGGGCAG CTCCGTGCCC GGCTACTGCC AGCCCATCAC CACTGTGACG 
GCTTCTGCCT CCGTGACTGT CGCCGTGCAC CCGCCGCCTG TCCCTGGGCC TGGGCGGAAC 
CCCCGAGGGG GACTCTGCCC AGGCTACCCT GAGACTGACC ACGGCCTGTT TGAGGACCCC 
CACGTGCCTT TCCACGTCCG GTGTGAGAGG AGGGATTCGA AGGTGGAAGT CATTGAGCTG 
CAGGACGTGG AATGCGAGGA GAGGCCCCGG GGAAGCAGCT CCAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Identification and characterization of two novel PTCH1 splice variants.
Biochemical and biophysical research communications487(1)68-75(2017 05)
Yu P,Yang J,Zhang Y

Hedgehog Signaling: From Basic Biology to Cancer Therapy.
Cell chemical biology24(3)252-280(2017 Mar)
Wu F,Zhang Y,Sun B,McMahon AP,Wang Y

Assessment of Promoter Methylation Identifies PTCH as a Putative Tumor-suppressor Gene in Human CLL.
Anticancer research36(9)4515-9(2016 09)
Schmidt-Wolf IG,Plass C,Byrd JC,Frevel K,Pietsch T,Waha A

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