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SFTPB cDNA ORF clone, Homo sapiens(Human)

Gene Symbol SFTPB
Entrez Gene ID 6439
Full Name surfactant protein B
Synonyms PSP-B,SFTB3,SFTP3,SMDP1,SP-B
General protein information
Preferred Names

surfactant protein B

Names

pulmonary surfactant-associated protein B
18 kDa pulmonary-surfactant protein
6 kDa protein
pulmonary surfactant-associated proteolipid SPL(Phe)

Gene Type protein-coding
Organism Homo sapiens(Human)
Genome

2

2p11.2

Summary This gene encodes the pulmonary-associated surfactant protein B (SPB), an amphipathic surfactant protein essential for lung function and homeostasis after birth. Pulmonary surfactant is a surface-active lipoprotein complex composed of 90% lipids and 10% proteins which include plasma proteins and apolipoproteins SPA, SPB, SPC and SPD. The surfactant is secreted by the alveolar cells of the lung and maintains the stability of pulmonary tissue by reducing the surface tension of fluids that coat the lung. The SPB enhances the rate of spreading and increases the stability of surfactant monolayers in vitro. Multiple mutations in this gene have been identified, which cause pulmonary surfactant metabolism dysfunction type 1, also called pulmonary alveolar proteinosis due to surfactant protein B deficiency, and are associated with fatal respiratory distress in the neonatal period. Alternatively spliced transcript variants encoding the same protein have been identified.[provided by RefSeq, Feb 2010].
Disorder MIM:

178640

Disorder Html: Surfactant metabolism dysfunction, pulmonary, 1, 265120 (3)

mRNA and Protein(s)

mRNA Protein Name
NM_000542.3 NP_000533.3 pulmonary surfactant-associated protein B precursor
XM_005264487.2 XP_005264544.1 pulmonary surfactant-associated protein B isoform X1
XM_005264488.3 XP_005264545.2 pulmonary surfactant-associated protein B isoform X2
XM_017004702.1 XP_016860191.1 pulmonary surfactant-associated protein B isoform X1
NM_198843.2 NP_942140.2 pulmonary surfactant-associated protein B precursor
XM_005264490.4 XP_005264547.2 pulmonary surfactant-associated protein B isoform X3
XM_017004703.1 XP_016860192.1 pulmonary surfactant-associated protein B isoform X2



Canis lupus familiaris (dog) SFTPB XP_005630540.1
Macaca mulatta (rhesus monkey) SFTPB XP_001089841.1
Pan troglodytes (chimpanzee) SFTPB XP_001168379.2
Mus musculus (house mouse) Sftpb NP_001269000.1
Homo sapiens (human) SFTPB NP_000533.3
Xenopus tropicalis (tropical clawed frog) sftpb XP_002932589.2
Bos taurus (cattle) SFTPB NP_001068779.1
Rattus norvegicus (Norway rat) Sftpb NP_620197.1


Related articles in PubMed

Genetic Polymorphisms of SP-A, SP-B, and SP-D and Risk of Respiratory Distress Syndrome in Preterm Neonates.
Chang HY, Li F, Li FS, Zheng CZ, Lei YZ, Wang J
Medical science monitor : international medical journal of experimental and clinical research225091-5100(2016 Dec)

Interindividual variability in the expression of surfactant protein A and B in the human lung during development.
Cau F, Pisu E, Gerosa C, Senes G, Ronchi F, Botta C, Di Felice E, Uda F, Marinelli V, Faa G, Fanos V, Moretti C, Fanni D
European journal of histochemistry : EJH60(3)2678(2016 Sep)

[Relationship between R236C site in exon 7 of SP-B gene and respiratory distress syndrome in Han newborns in western Inner Mongolia].
Wang J, Mei H, Liu CZ, Zhang YY, Liu CL, Song D, Zhang YH
Zhongguo dang dai er ke za zhi = Chinese journal of contemporary pediatrics18(9)802-805(2016 Sep)

[ASSOCIATION OF POLYMORPHISM GENES OF SURFACTANT PROTEINS IN PATIENTS WITH INFLUENZA].
Dudina KR, Kutateladze MM, Bokova NO, Znoiko OO, Abramov DD, Kelly EI, Yuschuk ND
Zhurnal mikrobiologii, epidemiologii, i immunobiologii(6)71-7(2015 Nov-Dec)

Amniotic fluid concentration of surfactant proteins in intra-amniotic infection.
Chaiworapongsa T, Hong JS, Hull WM, Romero R, Whitsett JA
The journal of maternal-fetal & neonatal medicine : the official journal of the European Association of Perinatal Medicine, the Federation of Asia and Oceania Perinatal Societies, the International Society of Perinatal Obstetricians21(9)663-70(2008 Sep)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

SP-A (+186A/G) and SP-B (1580C/T) polymorphisms are strongly associated with the risk of respiratory distress syndrome (RDS) in preterm infants. Notably, reduced serum SP-A levels were correlated with a high risk of RDS and may serve as novel biomarkers for RDS detection and monitoring.
Title: Genetic Polymorphisms of SP-A, SP-B, and SP-D and Risk of Respiratory Distress Syndrome in Preterm Neonates.

Differences were found regarding SPA and pro-SPB expression in the vast majority of subjects: in some lungs, SPA was more expressed whereas in others pro-SPB showed an higher degree of immunoreactivity. The expression of both surfactant proteins was not strictly correlated with gestational age.
Title: Interindividual variability in the expression of surfactant protein A and B in the human lung during development.

There is no significant association between the gene polymorphism of the R236C site in exon 7 of SP-B gene and the incidence ofneonatal respiratory distress syndrome (NRDS) in Han populations in that region.
Title: [Relationship between R236C site in exon 7 of SP-B gene and respiratory distress syndrome in Han newborns in western Inner Mongolia].

Data show that the thermal transitions observed in N-terminal propeptide of proSP-B (SP-BN) may correspond to the unfolding of certain protein domains or subdomains and/or to oligomers dissociation whereas the overall protein shows resistance to temperature-induced unfolding up to ~ 86 degrees C.
Title: Conformational Stability of the NH2-Terminal Propeptide of the Precursor of Pulmonary Surfactant Protein SP-B.

Significant differences in frequency of occurrence of unfavorable genotypes CC rs1965708, AA rs1059046 of SFTPA2 gene and CC rs1130866 of SFTPB gene in influenza patients in comparison with individuals of the control group were not detected.
Title: [ASSOCIATION OF POLYMORPHISM GENES OF SURFACTANT PROTEINS IN PATIENTS WITH INFLUENZA].

The following SFTPB gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the SFTPB cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

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***CloneID RefSeq Accession Definition **Vector *Turnaround time Price (USD) Select
OHu09313 NM_198843.2
Latest version!
Homo sapiens surfactant protein B (SFTPB), transcript variant 2, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $223.30
$319.00
OHu09313 NM_000542.3
Latest version!
Homo sapiens surfactant protein B (SFTPB), transcript variant 1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $223.30
$319.00
OHu09313 XM_017004702.1
Latest version!
Homo sapiens surfactant protein B (SFTPB), transcript variant X2, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $223.30
$319.00
OHu09313 XM_005264487.2
Latest version!
Homo sapiens surfactant protein B (SFTPB), transcript variant X1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $223.30
$319.00
OHu60453 XM_005264488.3
Latest version!
Homo sapiens surfactant protein B (SFTPB), transcript variant X3, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $223.30
$319.00
OHu60453 XM_017004703.1
Latest version!
Homo sapiens surfactant protein B (SFTPB), transcript variant X4, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $223.30
$319.00
OHu60454 XM_005264490.4
Latest version!
Homo sapiens surfactant protein B (SFTPB), transcript variant X5, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $223.30
$319.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.


CloneID OHu09313
Clone ID Related Accession (Same CDS sequence) NM_000542.3 , XM_005264487.2 , XM_017004702.1 , NM_198843.2
Accession Version NM_000542.3 Latest version! Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1182bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2017-05-06
Organism Homo sapiens(Human)
Product pulmonary surfactant-associated protein B precursor
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL548140.3, BC032785.1, BG482852.1, AB439563.1, CA437348.1 and AW204600.1. On Feb 13, 2010 this sequence version replaced NM_000542.2. Transcript Variant: This variant (1) is the longer transcript. Both variants 1 and 2 encode the same protein. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMEA2147920, SAMEA2155770 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
ATGCACCAAG CAGGGTACCC AGGCTGCAGA GGTGCCATGG CTGAGTCACA CCTGCTGCAG 
TGGCTGCTGC TGCTGCTGCC CACGCTCTGT GGCCCAGGCA CTGCTGCCTG GACCACCTCA
TCCTTGGCCT GTGCCCAGGG CCCTGAGTTC TGGTGCCAAA GCCTGGAGCA AGCATTGCAG
TGCAGAGCCC TAGGGCATTG CCTACAGGAA GTCTGGGGAC ATGTGGGAGC CGATGACCTA
TGCCAAGAGT GTGAGGACAT CGTCCACATC CTTAACAAGA TGGCCAAGGA GGCCATTTTC
CAGGACACGA TGAGGAAGTT CCTGGAGCAG GAGTGCAACG TCCTCCCCTT GAAGCTGCTC
ATGCCCCAGT GCAACCAAGT GCTTGACGAC TACTTCCCCC TGGTCATCGA CTACTTCCAG
AACCAGACTG ACTCAAACGG CATCTGTATG CACCTGGGCC TGTGCAAATC CCGGCAGCCA
GAGCCAGAGC AGGAGCCAGG GATGTCAGAC CCCCTGCCCA AACCTCTGCG GGACCCTCTG
CCAGACCCTC TGCTGGACAA GCTCGTCCTC CCTGTGCTGC CCGGGGCCCT CCAGGCGAGG
CCTGGGCCTC ACACACAGGA TCTCTCCGAG CAGCAATTCC CCATTCCTCT CCCCTATTGC
TGGCTCTGCA GGGCTCTGAT CAAGCGGATC CAAGCCATGA TTCCCAAGGG TGCGCTAGCT
GTGGCAGTGG CCCAGGTGTG CCGCGTGGTA CCTCTGGTGG CGGGCGGCAT CTGCCAGTGC
CTGGCTGAGC GCTACTCCGT CATCCTGCTC GACACGCTGC TGGGCCGCAT GCTGCCCCAG
CTGGTCTGCC GCCTCGTCCT CCGGTGCTCC ATGGATGACA GCGCTGGCCC AAGGTCGCCG
ACAGGAGAAT GGCTGCCGCG AGACTCTGAG TGCCACCTCT GCATGTCCGT GACCACCCAG
GCCGGGAACA GCAGCGAGCA GGCCATACCA CAGGCAATGC TCCAGGCCTG TGTTGGCTCC
TGGCTGGACA GGGAAAAGTG CAAGCAATTT GTGGAGCAGC ACACGCCCCA GCTGCTGACC
CTGGTGCCCA GGGGCTGGGA TGCCCACACC ACCTGCCAGG CCCTCGGGGT GTGTGGGACC
ATGTCCAGCC CTCTCCAGTG TATCCACAGC CCCGACCTTT GA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_000533.3
CDS101..1282
Misc Feature(1)92..94
Misc Feature(2)218..319
Misc Feature(3)335..565
Misc Feature(4)341..445
Misc Feature(5)470..565
Misc Feature(6)752..967
Misc Feature(7)1025..1234
Misc Feature(8)1139..1234
Exon (1)1..68
Exon (2)69..203
Exon (3)204..331
Exon (4)332..403
Exon (5)404..529
Exon (6)530..718
Exon (7)719..808
Exon (8)809..992
Exon (9)993..1138
Exon (10)1139..1219
Exon (11)1220..1301
Exon (12)1302..3667
Translation

Target ORF information:

RefSeq Version NM_000542.3
Organism Homo sapiens(Human)
Definition Homo sapiens surfactant protein B (SFTPB), transcript variant 1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_000542.3

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
ATGCACCAAG CAGGGTACCC AGGCTGCAGA GGTGCCATGG CTGAGTCACA CCTGCTGCAG 
TGGCTGCTGC TGCTGCTGCC CACGCTCTGT GGCCCAGGCA CTGCTGCCTG GACCACCTCA
TCCTTGGCCT GTGCCCAGGG CCCTGAGTTC TGGTGCCAAA GCCTGGAGCA AGCATTGCAG
TGCAGAGCCC TAGGGCATTG CCTACAGGAA GTCTGGGGAC ATGTGGGAGC CGATGACCTA
TGCCAAGAGT GTGAGGACAT CGTCCACATC CTTAACAAGA TGGCCAAGGA GGCCATTTTC
CAGGACACGA TGAGGAAGTT CCTGGAGCAG GAGTGCAACG TCCTCCCCTT GAAGCTGCTC
ATGCCCCAGT GCAACCAAGT GCTTGACGAC TACTTCCCCC TGGTCATCGA CTACTTCCAG
AACCAGACTG ACTCAAACGG CATCTGTATG CACCTGGGCC TGTGCAAATC CCGGCAGCCA
GAGCCAGAGC AGGAGCCAGG GATGTCAGAC CCCCTGCCCA AACCTCTGCG GGACCCTCTG
CCAGACCCTC TGCTGGACAA GCTCGTCCTC CCTGTGCTGC CCGGGGCCCT CCAGGCGAGG
CCTGGGCCTC ACACACAGGA TCTCTCCGAG CAGCAATTCC CCATTCCTCT CCCCTATTGC
TGGCTCTGCA GGGCTCTGAT CAAGCGGATC CAAGCCATGA TTCCCAAGGG TGCGCTAGCT
GTGGCAGTGG CCCAGGTGTG CCGCGTGGTA CCTCTGGTGG CGGGCGGCAT CTGCCAGTGC
CTGGCTGAGC GCTACTCCGT CATCCTGCTC GACACGCTGC TGGGCCGCAT GCTGCCCCAG
CTGGTCTGCC GCCTCGTCCT CCGGTGCTCC ATGGATGACA GCGCTGGCCC AAGGTCGCCG
ACAGGAGAAT GGCTGCCGCG AGACTCTGAG TGCCACCTCT GCATGTCCGT GACCACCCAG
GCCGGGAACA GCAGCGAGCA GGCCATACCA CAGGCAATGC TCCAGGCCTG TGTTGGCTCC
TGGCTGGACA GGGAAAAGTG CAAGCAATTT GTGGAGCAGC ACACGCCCCA GCTGCTGACC
CTGGTGCCCA GGGGCTGGGA TGCCCACACC ACCTGCCAGG CCCTCGGGGT GTGTGGGACC
ATGTCCAGCC CTCTCCAGTG TATCCACAGC CCCGACCTTT GA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu09313
Clone ID Related Accession (Same CDS sequence) NM_000542.3 , XM_005264487.2 , XM_017004702.1 , NM_198843.2
Accession Version NM_198843.2 Latest version! Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1182bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2017-05-06
Organism Homo sapiens(Human)
Product pulmonary surfactant-associated protein B precursor
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL548140.3, BC032785.1, AB439563.1, CA437348.1 and AW204600.1. This sequence is a reference standard in the RefSeqGene project. On Feb 13, 2010 this sequence version replaced NM_198843.1. Transcript Variant: This variant (2) lacks an internal segment in the 3' UTR, as compared to variant 1. Both variants 1 and 2 encode the same protein. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMEA2147920, SAMEA2155770 [ECO:0000348] ##Evidence-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
ATGCACCAAG CAGGGTACCC AGGCTGCAGA GGTGCCATGG CTGAGTCACA CCTGCTGCAG 
TGGCTGCTGC TGCTGCTGCC CACGCTCTGT GGCCCAGGCA CTGCTGCCTG GACCACCTCA
TCCTTGGCCT GTGCCCAGGG CCCTGAGTTC TGGTGCCAAA GCCTGGAGCA AGCATTGCAG
TGCAGAGCCC TAGGGCATTG CCTACAGGAA GTCTGGGGAC ATGTGGGAGC CGATGACCTA
TGCCAAGAGT GTGAGGACAT CGTCCACATC CTTAACAAGA TGGCCAAGGA GGCCATTTTC
CAGGACACGA TGAGGAAGTT CCTGGAGCAG GAGTGCAACG TCCTCCCCTT GAAGCTGCTC
ATGCCCCAGT GCAACCAAGT GCTTGACGAC TACTTCCCCC TGGTCATCGA CTACTTCCAG
AACCAGACTG ACTCAAACGG CATCTGTATG CACCTGGGCC TGTGCAAATC CCGGCAGCCA
GAGCCAGAGC AGGAGCCAGG GATGTCAGAC CCCCTGCCCA AACCTCTGCG GGACCCTCTG
CCAGACCCTC TGCTGGACAA GCTCGTCCTC CCTGTGCTGC CCGGGGCCCT CCAGGCGAGG
CCTGGGCCTC ACACACAGGA TCTCTCCGAG CAGCAATTCC CCATTCCTCT CCCCTATTGC
TGGCTCTGCA GGGCTCTGAT CAAGCGGATC CAAGCCATGA TTCCCAAGGG TGCGCTAGCT
GTGGCAGTGG CCCAGGTGTG CCGCGTGGTA CCTCTGGTGG CGGGCGGCAT CTGCCAGTGC
CTGGCTGAGC GCTACTCCGT CATCCTGCTC GACACGCTGC TGGGCCGCAT GCTGCCCCAG
CTGGTCTGCC GCCTCGTCCT CCGGTGCTCC ATGGATGACA GCGCTGGCCC AAGGTCGCCG
ACAGGAGAAT GGCTGCCGCG AGACTCTGAG TGCCACCTCT GCATGTCCGT GACCACCCAG
GCCGGGAACA GCAGCGAGCA GGCCATACCA CAGGCAATGC TCCAGGCCTG TGTTGGCTCC
TGGCTGGACA GGGAAAAGTG CAAGCAATTT GTGGAGCAGC ACACGCCCCA GCTGCTGACC
CTGGTGCCCA GGGGCTGGGA TGCCCACACC ACCTGCCAGG CCCTCGGGGT GTGTGGGACC
ATGTCCAGCC CTCTCCAGTG TATCCACAGC CCCGACCTTT GA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_942140.2
CDS101..1282
Misc Feature(1)92..94
Misc Feature(2)218..319
Misc Feature(3)335..565
Misc Feature(4)341..445
Misc Feature(5)470..565
Misc Feature(6)752..967
Misc Feature(7)1025..1234
Misc Feature(8)1139..1234
Exon (1)1..68
Exon (2)69..203
Exon (3)204..331
Exon (4)332..403
Exon (5)404..529
Exon (6)530..718
Exon (7)719..808
Exon (8)809..992
Exon (9)993..1138
Exon (10)1139..1219
Exon (11)1220..1301
Exon (12)1302..2840
Translation

Target ORF information:

RefSeq Version NM_198843.2
Organism Homo sapiens(Human)
Definition Homo sapiens surfactant protein B (SFTPB), transcript variant 2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_198843.2

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
ATGCACCAAG CAGGGTACCC AGGCTGCAGA GGTGCCATGG CTGAGTCACA CCTGCTGCAG 
TGGCTGCTGC TGCTGCTGCC CACGCTCTGT GGCCCAGGCA CTGCTGCCTG GACCACCTCA
TCCTTGGCCT GTGCCCAGGG CCCTGAGTTC TGGTGCCAAA GCCTGGAGCA AGCATTGCAG
TGCAGAGCCC TAGGGCATTG CCTACAGGAA GTCTGGGGAC ATGTGGGAGC CGATGACCTA
TGCCAAGAGT GTGAGGACAT CGTCCACATC CTTAACAAGA TGGCCAAGGA GGCCATTTTC
CAGGACACGA TGAGGAAGTT CCTGGAGCAG GAGTGCAACG TCCTCCCCTT GAAGCTGCTC
ATGCCCCAGT GCAACCAAGT GCTTGACGAC TACTTCCCCC TGGTCATCGA CTACTTCCAG
AACCAGACTG ACTCAAACGG CATCTGTATG CACCTGGGCC TGTGCAAATC CCGGCAGCCA
GAGCCAGAGC AGGAGCCAGG GATGTCAGAC CCCCTGCCCA AACCTCTGCG GGACCCTCTG
CCAGACCCTC TGCTGGACAA GCTCGTCCTC CCTGTGCTGC CCGGGGCCCT CCAGGCGAGG
CCTGGGCCTC ACACACAGGA TCTCTCCGAG CAGCAATTCC CCATTCCTCT CCCCTATTGC
TGGCTCTGCA GGGCTCTGAT CAAGCGGATC CAAGCCATGA TTCCCAAGGG TGCGCTAGCT
GTGGCAGTGG CCCAGGTGTG CCGCGTGGTA CCTCTGGTGG CGGGCGGCAT CTGCCAGTGC
CTGGCTGAGC GCTACTCCGT CATCCTGCTC GACACGCTGC TGGGCCGCAT GCTGCCCCAG
CTGGTCTGCC GCCTCGTCCT CCGGTGCTCC ATGGATGACA GCGCTGGCCC AAGGTCGCCG
ACAGGAGAAT GGCTGCCGCG AGACTCTGAG TGCCACCTCT GCATGTCCGT GACCACCCAG
GCCGGGAACA GCAGCGAGCA GGCCATACCA CAGGCAATGC TCCAGGCCTG TGTTGGCTCC
TGGCTGGACA GGGAAAAGTG CAAGCAATTT GTGGAGCAGC ACACGCCCCA GCTGCTGACC
CTGGTGCCCA GGGGCTGGGA TGCCCACACC ACCTGCCAGG CCCTCGGGGT GTGTGGGACC
ATGTCCAGCC CTCTCCAGTG TATCCACAGC CCCGACCTTT GA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu09313
Clone ID Related Accession (Same CDS sequence) NM_000542.3 , XM_005264487.2 , XM_017004702.1 , NM_198843.2
Accession Version XM_005264487.2 Latest version! Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1182bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2016-06-06
Organism Homo sapiens(Human)
Product pulmonary surfactant-associated protein B isoform X1
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000002.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 12, 2015 this sequence version replaced XM_005264487.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
ATGCACCAAG CAGGGTACCC AGGCTGCAGA GGTGCCATGG CTGAGTCACA CCTGCTGCAG 
TGGCTGCTGC TGCTGCTGCC CACGCTCTGT GGCCCAGGCA CTGCTGCCTG GACCACCTCA
TCCTTGGCCT GTGCCCAGGG CCCTGAGTTC TGGTGCCAAA GCCTGGAGCA AGCATTGCAG
TGCAGAGCCC TAGGGCATTG CCTACAGGAA GTCTGGGGAC ATGTGGGAGC CGATGACCTA
TGCCAAGAGT GTGAGGACAT CGTCCACATC CTTAACAAGA TGGCCAAGGA GGCCATTTTC
CAGGACACGA TGAGGAAGTT CCTGGAGCAG GAGTGCAACG TCCTCCCCTT GAAGCTGCTC
ATGCCCCAGT GCAACCAAGT GCTTGACGAC TACTTCCCCC TGGTCATCGA CTACTTCCAG
AACCAGACTG ACTCAAACGG CATCTGTATG CACCTGGGCC TGTGCAAATC CCGGCAGCCA
GAGCCAGAGC AGGAGCCAGG GATGTCAGAC CCCCTGCCCA AACCTCTGCG GGACCCTCTG
CCAGACCCTC TGCTGGACAA GCTCGTCCTC CCTGTGCTGC CCGGGGCCCT CCAGGCGAGG
CCTGGGCCTC ACACACAGGA TCTCTCCGAG CAGCAATTCC CCATTCCTCT CCCCTATTGC
TGGCTCTGCA GGGCTCTGAT CAAGCGGATC CAAGCCATGA TTCCCAAGGG TGCGCTAGCT
GTGGCAGTGG CCCAGGTGTG CCGCGTGGTA CCTCTGGTGG CGGGCGGCAT CTGCCAGTGC
CTGGCTGAGC GCTACTCCGT CATCCTGCTC GACACGCTGC TGGGCCGCAT GCTGCCCCAG
CTGGTCTGCC GCCTCGTCCT CCGGTGCTCC ATGGATGACA GCGCTGGCCC AAGGTCGCCG
ACAGGAGAAT GGCTGCCGCG AGACTCTGAG TGCCACCTCT GCATGTCCGT GACCACCCAG
GCCGGGAACA GCAGCGAGCA GGCCATACCA CAGGCAATGC TCCAGGCCTG TGTTGGCTCC
TGGCTGGACA GGGAAAAGTG CAAGCAATTT GTGGAGCAGC ACACGCCCCA GCTGCTGACC
CTGGTGCCCA GGGGCTGGGA TGCCCACACC ACCTGCCAGG CCCTCGGGGT GTGTGGGACC
ATGTCCAGCC CTCTCCAGTG TATCCACAGC CCCGACCTTT GA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_005264544.1
CDS16..1197
Misc Feature(1)133..234
Misc Feature(2)250..480
Misc Feature(3)256..360
Misc Feature(4)385..480
Misc Feature(5)667..882
Misc Feature(6)940..1149
Misc Feature(7)1054..1149
Translation

Target ORF information:

RefSeq Version XM_005264487.2
Organism Homo sapiens(Human)
Definition Homo sapiens surfactant protein B (SFTPB), transcript variant X1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_005264487.2

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
ATGCACCAAG CAGGGTACCC AGGCTGCAGA GGTGCCATGG CTGAGTCACA CCTGCTGCAG 
TGGCTGCTGC TGCTGCTGCC CACGCTCTGT GGCCCAGGCA CTGCTGCCTG GACCACCTCA
TCCTTGGCCT GTGCCCAGGG CCCTGAGTTC TGGTGCCAAA GCCTGGAGCA AGCATTGCAG
TGCAGAGCCC TAGGGCATTG CCTACAGGAA GTCTGGGGAC ATGTGGGAGC CGATGACCTA
TGCCAAGAGT GTGAGGACAT CGTCCACATC CTTAACAAGA TGGCCAAGGA GGCCATTTTC
CAGGACACGA TGAGGAAGTT CCTGGAGCAG GAGTGCAACG TCCTCCCCTT GAAGCTGCTC
ATGCCCCAGT GCAACCAAGT GCTTGACGAC TACTTCCCCC TGGTCATCGA CTACTTCCAG
AACCAGACTG ACTCAAACGG CATCTGTATG CACCTGGGCC TGTGCAAATC CCGGCAGCCA
GAGCCAGAGC AGGAGCCAGG GATGTCAGAC CCCCTGCCCA AACCTCTGCG GGACCCTCTG
CCAGACCCTC TGCTGGACAA GCTCGTCCTC CCTGTGCTGC CCGGGGCCCT CCAGGCGAGG
CCTGGGCCTC ACACACAGGA TCTCTCCGAG CAGCAATTCC CCATTCCTCT CCCCTATTGC
TGGCTCTGCA GGGCTCTGAT CAAGCGGATC CAAGCCATGA TTCCCAAGGG TGCGCTAGCT
GTGGCAGTGG CCCAGGTGTG CCGCGTGGTA CCTCTGGTGG CGGGCGGCAT CTGCCAGTGC
CTGGCTGAGC GCTACTCCGT CATCCTGCTC GACACGCTGC TGGGCCGCAT GCTGCCCCAG
CTGGTCTGCC GCCTCGTCCT CCGGTGCTCC ATGGATGACA GCGCTGGCCC AAGGTCGCCG
ACAGGAGAAT GGCTGCCGCG AGACTCTGAG TGCCACCTCT GCATGTCCGT GACCACCCAG
GCCGGGAACA GCAGCGAGCA GGCCATACCA CAGGCAATGC TCCAGGCCTG TGTTGGCTCC
TGGCTGGACA GGGAAAAGTG CAAGCAATTT GTGGAGCAGC ACACGCCCCA GCTGCTGACC
CTGGTGCCCA GGGGCTGGGA TGCCCACACC ACCTGCCAGG CCCTCGGGGT GTGTGGGACC
ATGTCCAGCC CTCTCCAGTG TATCCACAGC CCCGACCTTT GA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu09313
Clone ID Related Accession (Same CDS sequence) NM_000542.3 , XM_005264487.2 , XM_017004702.1 , NM_198843.2
Accession Version XM_017004702.1 Latest version! Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1182bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2016-06-06
Organism Homo sapiens(Human)
Product pulmonary surfactant-associated protein B isoform X1
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000002.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
ATGCACCAAG CAGGGTACCC AGGCTGCAGA GGTGCCATGG CTGAGTCACA CCTGCTGCAG 
TGGCTGCTGC TGCTGCTGCC CACGCTCTGT GGCCCAGGCA CTGCTGCCTG GACCACCTCA
TCCTTGGCCT GTGCCCAGGG CCCTGAGTTC TGGTGCCAAA GCCTGGAGCA AGCATTGCAG
TGCAGAGCCC TAGGGCATTG CCTACAGGAA GTCTGGGGAC ATGTGGGAGC CGATGACCTA
TGCCAAGAGT GTGAGGACAT CGTCCACATC CTTAACAAGA TGGCCAAGGA GGCCATTTTC
CAGGACACGA TGAGGAAGTT CCTGGAGCAG GAGTGCAACG TCCTCCCCTT GAAGCTGCTC
ATGCCCCAGT GCAACCAAGT GCTTGACGAC TACTTCCCCC TGGTCATCGA CTACTTCCAG
AACCAGACTG ACTCAAACGG CATCTGTATG CACCTGGGCC TGTGCAAATC CCGGCAGCCA
GAGCCAGAGC AGGAGCCAGG GATGTCAGAC CCCCTGCCCA AACCTCTGCG GGACCCTCTG
CCAGACCCTC TGCTGGACAA GCTCGTCCTC CCTGTGCTGC CCGGGGCCCT CCAGGCGAGG
CCTGGGCCTC ACACACAGGA TCTCTCCGAG CAGCAATTCC CCATTCCTCT CCCCTATTGC
TGGCTCTGCA GGGCTCTGAT CAAGCGGATC CAAGCCATGA TTCCCAAGGG TGCGCTAGCT
GTGGCAGTGG CCCAGGTGTG CCGCGTGGTA CCTCTGGTGG CGGGCGGCAT CTGCCAGTGC
CTGGCTGAGC GCTACTCCGT CATCCTGCTC GACACGCTGC TGGGCCGCAT GCTGCCCCAG
CTGGTCTGCC GCCTCGTCCT CCGGTGCTCC ATGGATGACA GCGCTGGCCC AAGGTCGCCG
ACAGGAGAAT GGCTGCCGCG AGACTCTGAG TGCCACCTCT GCATGTCCGT GACCACCCAG
GCCGGGAACA GCAGCGAGCA GGCCATACCA CAGGCAATGC TCCAGGCCTG TGTTGGCTCC
TGGCTGGACA GGGAAAAGTG CAAGCAATTT GTGGAGCAGC ACACGCCCCA GCTGCTGACC
CTGGTGCCCA GGGGCTGGGA TGCCCACACC ACCTGCCAGG CCCTCGGGGT GTGTGGGACC
ATGTCCAGCC CTCTCCAGTG TATCCACAGC CCCGACCTTT GA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_016860191.1
CDS16..1197
Misc Feature(1)133..234
Misc Feature(2)250..480
Misc Feature(3)256..360
Misc Feature(4)385..480
Misc Feature(5)667..882
Misc Feature(6)940..1149
Misc Feature(7)1054..1149
Translation

Target ORF information:

RefSeq Version XM_017004702.1
Organism Homo sapiens(Human)
Definition Homo sapiens surfactant protein B (SFTPB), transcript variant X2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_017004702.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
ATGCACCAAG CAGGGTACCC AGGCTGCAGA GGTGCCATGG CTGAGTCACA CCTGCTGCAG 
TGGCTGCTGC TGCTGCTGCC CACGCTCTGT GGCCCAGGCA CTGCTGCCTG GACCACCTCA
TCCTTGGCCT GTGCCCAGGG CCCTGAGTTC TGGTGCCAAA GCCTGGAGCA AGCATTGCAG
TGCAGAGCCC TAGGGCATTG CCTACAGGAA GTCTGGGGAC ATGTGGGAGC CGATGACCTA
TGCCAAGAGT GTGAGGACAT CGTCCACATC CTTAACAAGA TGGCCAAGGA GGCCATTTTC
CAGGACACGA TGAGGAAGTT CCTGGAGCAG GAGTGCAACG TCCTCCCCTT GAAGCTGCTC
ATGCCCCAGT GCAACCAAGT GCTTGACGAC TACTTCCCCC TGGTCATCGA CTACTTCCAG
AACCAGACTG ACTCAAACGG CATCTGTATG CACCTGGGCC TGTGCAAATC CCGGCAGCCA
GAGCCAGAGC AGGAGCCAGG GATGTCAGAC CCCCTGCCCA AACCTCTGCG GGACCCTCTG
CCAGACCCTC TGCTGGACAA GCTCGTCCTC CCTGTGCTGC CCGGGGCCCT CCAGGCGAGG
CCTGGGCCTC ACACACAGGA TCTCTCCGAG CAGCAATTCC CCATTCCTCT CCCCTATTGC
TGGCTCTGCA GGGCTCTGAT CAAGCGGATC CAAGCCATGA TTCCCAAGGG TGCGCTAGCT
GTGGCAGTGG CCCAGGTGTG CCGCGTGGTA CCTCTGGTGG CGGGCGGCAT CTGCCAGTGC
CTGGCTGAGC GCTACTCCGT CATCCTGCTC GACACGCTGC TGGGCCGCAT GCTGCCCCAG
CTGGTCTGCC GCCTCGTCCT CCGGTGCTCC ATGGATGACA GCGCTGGCCC AAGGTCGCCG
ACAGGAGAAT GGCTGCCGCG AGACTCTGAG TGCCACCTCT GCATGTCCGT GACCACCCAG
GCCGGGAACA GCAGCGAGCA GGCCATACCA CAGGCAATGC TCCAGGCCTG TGTTGGCTCC
TGGCTGGACA GGGAAAAGTG CAAGCAATTT GTGGAGCAGC ACACGCCCCA GCTGCTGACC
CTGGTGCCCA GGGGCTGGGA TGCCCACACC ACCTGCCAGG CCCTCGGGGT GTGTGGGACC
ATGTCCAGCC CTCTCCAGTG TATCCACAGC CCCGACCTTT GA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu60453
Clone ID Related Accession (Same CDS sequence) XM_005264488.3 , XM_017004703.1
Accession Version XM_005264488.3 Latest version! Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1134bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2016-06-06
Organism Homo sapiens(Human)
Product pulmonary surfactant-associated protein B isoform X2
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000002.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_005264488.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
ATGGCTGAGT CACACCTGCT GCAGTGGCTG CTGCTGCTGC TGCCCACGCT CTGTGGCCCA 
GGCACTGCTG CCTGGACCAC CTCATCCTTG GCCTGTGCCC AGGGCCCTGA GTTCTGGTGC
CAAAGCCTGG AGCAAGCATT GCAGTGCAGA GCCCTAGGGC ATTGCCTACA GGAAGTCTGG
GGACATGTGG GAGCCGATGA CCTATGCCAA GAGTGTGAGG ACATCGTCCA CATCCTTAAC
AAGATGGCCA AGGAGGCCAT TTTCCAGGAC ACGATGAGGA AGTTCCTGGA GCAGGAGTGC
AACGTCCTCC CCTTGAAGCT GCTCATGCCC CAGTGCAACC AAGTGCTTGA CGACTACTTC
CCCCTGGTCA TCGACTACTT CCAGAACCAG ACTGACTCAA ACGGCATCTG TATGCACCTG
GGCCTGTGCA AATCCCGGCA GCCAGAGCCA GAGCAGGAGC CAGGGATGTC AGACCCCCTG
CCCAAACCTC TGCGGGACCC TCTGCCAGAC CCTCTGCTGG ACAAGCTCGT CCTCCCTGTG
CTGCCCGGGG CCCTCCAGGC GAGGCCTGGG CCTCACACAC AGGATCTCTC CGAGCAGCAA
TTCCCCATTC CTCTCCCCTA TTGCTGGCTC TGCAGGGCTC TGATCAAGCG GATCCAAGCC
ATGATTCCCA AGGGTGCGCT AGCTGTGGCA GTGGCCCAGG TGTGCCGCGT GGTACCTCTG
GTGGCGGGCG GCATCTGCCA GTGCCTGGCT GAGCGCTACT CCGTCATCCT GCTCGACACG
CTGCTGGGCC GCATGCTGCC CCAGCTGGTC TGCCGCCTCG TCCTCCGGTG CTCCATGGAT
GACAGCGCTG GCCCAAGAGA ATGGCTGCCG CGAGACTCTG AGTGCCACCT CTGCATGTCC
GTGACCACCC AGGCCGGGAA CAGCAGCGAG CAGGCCATAC CACAGGCAAT GCTCCAGGCC
TGTGTTGGCT CCTGGCTGGA CAGGGAAAAG TGCAAGCAAT TTGTGGAGCA GCACACGCCC
CAGCTGCTGA CCCTGGTGCC CAGGGGCTGG GATGCCCACA CCACCTGCCA GGCCCTCGGG
GTGTGTGGGA CCATGTCCAG CCCTCTCCAG TGTATCCACA GCCCCGACCT TTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_005264545.2
CDS17..1150
Misc Feature(1)98..199
Misc Feature(2)215..445
Misc Feature(3)221..325
Misc Feature(4)350..445
Misc Feature(5)632..847
Misc Feature(6)893..1102
Misc Feature(7)1007..1102
Translation

Target ORF information:

RefSeq Version XM_005264488.3
Organism Homo sapiens(Human)
Definition Homo sapiens surfactant protein B (SFTPB), transcript variant X3, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_005264488.3

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
ATGGCTGAGT CACACCTGCT GCAGTGGCTG CTGCTGCTGC TGCCCACGCT CTGTGGCCCA 
GGCACTGCTG CCTGGACCAC CTCATCCTTG GCCTGTGCCC AGGGCCCTGA GTTCTGGTGC
CAAAGCCTGG AGCAAGCATT GCAGTGCAGA GCCCTAGGGC ATTGCCTACA GGAAGTCTGG
GGACATGTGG GAGCCGATGA CCTATGCCAA GAGTGTGAGG ACATCGTCCA CATCCTTAAC
AAGATGGCCA AGGAGGCCAT TTTCCAGGAC ACGATGAGGA AGTTCCTGGA GCAGGAGTGC
AACGTCCTCC CCTTGAAGCT GCTCATGCCC CAGTGCAACC AAGTGCTTGA CGACTACTTC
CCCCTGGTCA TCGACTACTT CCAGAACCAG ACTGACTCAA ACGGCATCTG TATGCACCTG
GGCCTGTGCA AATCCCGGCA GCCAGAGCCA GAGCAGGAGC CAGGGATGTC AGACCCCCTG
CCCAAACCTC TGCGGGACCC TCTGCCAGAC CCTCTGCTGG ACAAGCTCGT CCTCCCTGTG
CTGCCCGGGG CCCTCCAGGC GAGGCCTGGG CCTCACACAC AGGATCTCTC CGAGCAGCAA
TTCCCCATTC CTCTCCCCTA TTGCTGGCTC TGCAGGGCTC TGATCAAGCG GATCCAAGCC
ATGATTCCCA AGGGTGCGCT AGCTGTGGCA GTGGCCCAGG TGTGCCGCGT GGTACCTCTG
GTGGCGGGCG GCATCTGCCA GTGCCTGGCT GAGCGCTACT CCGTCATCCT GCTCGACACG
CTGCTGGGCC GCATGCTGCC CCAGCTGGTC TGCCGCCTCG TCCTCCGGTG CTCCATGGAT
GACAGCGCTG GCCCAAGAGA ATGGCTGCCG CGAGACTCTG AGTGCCACCT CTGCATGTCC
GTGACCACCC AGGCCGGGAA CAGCAGCGAG CAGGCCATAC CACAGGCAAT GCTCCAGGCC
TGTGTTGGCT CCTGGCTGGA CAGGGAAAAG TGCAAGCAAT TTGTGGAGCA GCACACGCCC
CAGCTGCTGA CCCTGGTGCC CAGGGGCTGG GATGCCCACA CCACCTGCCA GGCCCTCGGG
GTGTGTGGGA CCATGTCCAG CCCTCTCCAG TGTATCCACA GCCCCGACCT TTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu60453
Clone ID Related Accession (Same CDS sequence) XM_005264488.3 , XM_017004703.1
Accession Version XM_017004703.1 Latest version! Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1134bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2016-06-06
Organism Homo sapiens(Human)
Product pulmonary surfactant-associated protein B isoform X2
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000002.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
ATGGCTGAGT CACACCTGCT GCAGTGGCTG CTGCTGCTGC TGCCCACGCT CTGTGGCCCA 
GGCACTGCTG CCTGGACCAC CTCATCCTTG GCCTGTGCCC AGGGCCCTGA GTTCTGGTGC
CAAAGCCTGG AGCAAGCATT GCAGTGCAGA GCCCTAGGGC ATTGCCTACA GGAAGTCTGG
GGACATGTGG GAGCCGATGA CCTATGCCAA GAGTGTGAGG ACATCGTCCA CATCCTTAAC
AAGATGGCCA AGGAGGCCAT TTTCCAGGAC ACGATGAGGA AGTTCCTGGA GCAGGAGTGC
AACGTCCTCC CCTTGAAGCT GCTCATGCCC CAGTGCAACC AAGTGCTTGA CGACTACTTC
CCCCTGGTCA TCGACTACTT CCAGAACCAG ACTGACTCAA ACGGCATCTG TATGCACCTG
GGCCTGTGCA AATCCCGGCA GCCAGAGCCA GAGCAGGAGC CAGGGATGTC AGACCCCCTG
CCCAAACCTC TGCGGGACCC TCTGCCAGAC CCTCTGCTGG ACAAGCTCGT CCTCCCTGTG
CTGCCCGGGG CCCTCCAGGC GAGGCCTGGG CCTCACACAC AGGATCTCTC CGAGCAGCAA
TTCCCCATTC CTCTCCCCTA TTGCTGGCTC TGCAGGGCTC TGATCAAGCG GATCCAAGCC
ATGATTCCCA AGGGTGCGCT AGCTGTGGCA GTGGCCCAGG TGTGCCGCGT GGTACCTCTG
GTGGCGGGCG GCATCTGCCA GTGCCTGGCT GAGCGCTACT CCGTCATCCT GCTCGACACG
CTGCTGGGCC GCATGCTGCC CCAGCTGGTC TGCCGCCTCG TCCTCCGGTG CTCCATGGAT
GACAGCGCTG GCCCAAGAGA ATGGCTGCCG CGAGACTCTG AGTGCCACCT CTGCATGTCC
GTGACCACCC AGGCCGGGAA CAGCAGCGAG CAGGCCATAC CACAGGCAAT GCTCCAGGCC
TGTGTTGGCT CCTGGCTGGA CAGGGAAAAG TGCAAGCAAT TTGTGGAGCA GCACACGCCC
CAGCTGCTGA CCCTGGTGCC CAGGGGCTGG GATGCCCACA CCACCTGCCA GGCCCTCGGG
GTGTGTGGGA CCATGTCCAG CCCTCTCCAG TGTATCCACA GCCCCGACCT TTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_016860192.1
CDS17..1150
Misc Feature(1)98..199
Misc Feature(2)215..445
Misc Feature(3)221..325
Misc Feature(4)350..445
Misc Feature(5)632..847
Misc Feature(6)893..1102
Misc Feature(7)1007..1102
Translation

Target ORF information:

RefSeq Version XM_017004703.1
Organism Homo sapiens(Human)
Definition Homo sapiens surfactant protein B (SFTPB), transcript variant X4, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_017004703.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
ATGGCTGAGT CACACCTGCT GCAGTGGCTG CTGCTGCTGC TGCCCACGCT CTGTGGCCCA 
GGCACTGCTG CCTGGACCAC CTCATCCTTG GCCTGTGCCC AGGGCCCTGA GTTCTGGTGC
CAAAGCCTGG AGCAAGCATT GCAGTGCAGA GCCCTAGGGC ATTGCCTACA GGAAGTCTGG
GGACATGTGG GAGCCGATGA CCTATGCCAA GAGTGTGAGG ACATCGTCCA CATCCTTAAC
AAGATGGCCA AGGAGGCCAT TTTCCAGGAC ACGATGAGGA AGTTCCTGGA GCAGGAGTGC
AACGTCCTCC CCTTGAAGCT GCTCATGCCC CAGTGCAACC AAGTGCTTGA CGACTACTTC
CCCCTGGTCA TCGACTACTT CCAGAACCAG ACTGACTCAA ACGGCATCTG TATGCACCTG
GGCCTGTGCA AATCCCGGCA GCCAGAGCCA GAGCAGGAGC CAGGGATGTC AGACCCCCTG
CCCAAACCTC TGCGGGACCC TCTGCCAGAC CCTCTGCTGG ACAAGCTCGT CCTCCCTGTG
CTGCCCGGGG CCCTCCAGGC GAGGCCTGGG CCTCACACAC AGGATCTCTC CGAGCAGCAA
TTCCCCATTC CTCTCCCCTA TTGCTGGCTC TGCAGGGCTC TGATCAAGCG GATCCAAGCC
ATGATTCCCA AGGGTGCGCT AGCTGTGGCA GTGGCCCAGG TGTGCCGCGT GGTACCTCTG
GTGGCGGGCG GCATCTGCCA GTGCCTGGCT GAGCGCTACT CCGTCATCCT GCTCGACACG
CTGCTGGGCC GCATGCTGCC CCAGCTGGTC TGCCGCCTCG TCCTCCGGTG CTCCATGGAT
GACAGCGCTG GCCCAAGAGA ATGGCTGCCG CGAGACTCTG AGTGCCACCT CTGCATGTCC
GTGACCACCC AGGCCGGGAA CAGCAGCGAG CAGGCCATAC CACAGGCAAT GCTCCAGGCC
TGTGTTGGCT CCTGGCTGGA CAGGGAAAAG TGCAAGCAAT TTGTGGAGCA GCACACGCCC
CAGCTGCTGA CCCTGGTGCC CAGGGGCTGG GATGCCCACA CCACCTGCCA GGCCCTCGGG
GTGTGTGGGA CCATGTCCAG CCCTCTCCAG TGTATCCACA GCCCCGACCT TTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu60454
Clone ID Related Accession (Same CDS sequence) XM_005264490.4
Accession Version XM_005264490.4 Latest version! Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1020bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2016-06-06
Organism Homo sapiens(Human)
Product pulmonary surfactant-associated protein B isoform X3
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000002.12) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_005264490.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
ATGGCTGAGT CACACCTGCT GCAGTGGCTG CTGCTGCTGC TGCCCACGCT CTGTGGCCCA 
GGCACTGCTG CCTGGACCAC CTCATCCTTG GCCTGTGCCC AGGGCCCTGA GTTCTGGTGC
CAAAGCCTGG AGCAAGCATT GCAGTGCAGA GCCCTAGGGC ATTGCCTACA GGAAGTCTGG
GGACATGTGG GAGCCGATGA CCTATGCCAA GAGTGTGAGG ACATCGTCCA CATCCTTAAC
AAGATGGCCA AGGAGGCCAT TTTCCAGGAC ACGATGAGGA AGTTCCTGGA GCAGGAGTGC
AACGTCCTCC CCTTGAAGCT GCTCATGCCC CAGTGCAACC AAGTGCTTGA CGACTACTTC
CCCCTGGTCA TCGACTACTT CCAGAACCAG ACTGACTCAA ACGGCATCTG TATGCACCTG
GGCCTGTGCA AATCCCGGCA GCCAGAGCCA GAGCAGGAGC CAGGGATGTC AGACCCCCTG
CCCAAACCTC TGCGGGACCC TCTGCCAGAC CCTCTGCTGG ACAAGCTCGT CCTCCCTGTG
CTGCCCGGGG CCCTCCAGGC GAGGCCTGGG CCTCACACAC AGGATCTCTC CGAGCAGCAA
TTCCCCATTC CTCTCCCCTA TTGCTGGCTC TGCAGGGCTC TGATCAAGCG GATCCAAGCC
ATGATTCCCA AGGGTGCGCT AGCTGTGGCA GTGGCCCAGG TGTGCCGCGT GGTACCTCTG
GTGGCGGGCG GCATCTGCCA GTGCCTGGCT GAGCGCTACT CCGTCATCCT GCTCGACACG
CTGCTGGGCC GCATGCTGCC CCAGCTGGTC TGCCGCCTCG TCCTCCGGTG CTCCATGGAT
GACAGCGCTG GCCCAAGGTC GCCGACAGGA GAATGGCTGC CGCGAGACTC TGAGTGCCAC
CTCTGCATGT CCGTGACCAC CCAGGCCGGG AACAGCAGCG AGCAGGCCAT ACCACAGGCA
ATGCTCCAGG CCTGTGTTGG CTCCTGGCTG GACAGGGAAA AGCTGGCTCC AGTTTGTTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_005264547.2
CDS17..1036
Misc Feature(1)98..199
Misc Feature(2)215..445
Misc Feature(3)221..325
Misc Feature(4)350..445
Misc Feature(5)632..847
Translation

Target ORF information:

RefSeq Version XM_005264490.4
Organism Homo sapiens(Human)
Definition Homo sapiens surfactant protein B (SFTPB), transcript variant X5, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_005264490.4

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
ATGGCTGAGT CACACCTGCT GCAGTGGCTG CTGCTGCTGC TGCCCACGCT CTGTGGCCCA 
GGCACTGCTG CCTGGACCAC CTCATCCTTG GCCTGTGCCC AGGGCCCTGA GTTCTGGTGC
CAAAGCCTGG AGCAAGCATT GCAGTGCAGA GCCCTAGGGC ATTGCCTACA GGAAGTCTGG
GGACATGTGG GAGCCGATGA CCTATGCCAA GAGTGTGAGG ACATCGTCCA CATCCTTAAC
AAGATGGCCA AGGAGGCCAT TTTCCAGGAC ACGATGAGGA AGTTCCTGGA GCAGGAGTGC
AACGTCCTCC CCTTGAAGCT GCTCATGCCC CAGTGCAACC AAGTGCTTGA CGACTACTTC
CCCCTGGTCA TCGACTACTT CCAGAACCAG ACTGACTCAA ACGGCATCTG TATGCACCTG
GGCCTGTGCA AATCCCGGCA GCCAGAGCCA GAGCAGGAGC CAGGGATGTC AGACCCCCTG
CCCAAACCTC TGCGGGACCC TCTGCCAGAC CCTCTGCTGG ACAAGCTCGT CCTCCCTGTG
CTGCCCGGGG CCCTCCAGGC GAGGCCTGGG CCTCACACAC AGGATCTCTC CGAGCAGCAA
TTCCCCATTC CTCTCCCCTA TTGCTGGCTC TGCAGGGCTC TGATCAAGCG GATCCAAGCC
ATGATTCCCA AGGGTGCGCT AGCTGTGGCA GTGGCCCAGG TGTGCCGCGT GGTACCTCTG
GTGGCGGGCG GCATCTGCCA GTGCCTGGCT GAGCGCTACT CCGTCATCCT GCTCGACACG
CTGCTGGGCC GCATGCTGCC CCAGCTGGTC TGCCGCCTCG TCCTCCGGTG CTCCATGGAT
GACAGCGCTG GCCCAAGGTC GCCGACAGGA GAATGGCTGC CGCGAGACTC TGAGTGCCAC
CTCTGCATGT CCGTGACCAC CCAGGCCGGG AACAGCAGCG AGCAGGCCAT ACCACAGGCA
ATGCTCCAGG CCTGTGTTGG CTCCTGGCTG GACAGGGAAA AGCTGGCTCC AGTTTGTTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

book

Genetic Polymorphisms of SP-A, SP-B, and SP-D and Risk of Respiratory Distress Syndrome in Preterm Neonates.
Medical science monitor : international medical journal of experimental and clinical research225091-5100(2016 Dec)
Chang HY,Li F,Li FS,Zheng CZ,Lei YZ,Wang J


book

Interindividual variability in the expression of surfactant protein A and B in the human lung during development.
European journal of histochemistry : EJH60(3)2678(2016 Sep)
Cau F,Pisu E,Gerosa C,Senes G,Ronchi F,Botta C,Di Felice E,Uda F,Marinelli V,Faa G,Fanos V,Moretti C,Fanni D


book

[Relationship between R236C site in exon 7 of SP-B gene and respiratory distress syndrome in Han newborns in western Inner Mongolia].
Zhongguo dang dai er ke za zhi = Chinese journal of contemporary pediatrics18(9)802-805(2016 Sep)
Wang J,Mei H,Liu CZ,Zhang YY,Liu CL,Song D,Zhang YH


book

[ASSOCIATION OF POLYMORPHISM GENES OF SURFACTANT PROTEINS IN PATIENTS WITH INFLUENZA].
Zhurnal mikrobiologii, epidemiologii, i immunobiologii(6)71-7(2015 Nov-Dec)
Dudina KR,Kutateladze MM,Bokova NO,Znoiko OO,Abramov DD,Kelly EI,Yuschuk ND


book

Amniotic fluid concentration of surfactant proteins in intra-amniotic infection.
The journal of maternal-fetal & neonatal medicine : the official journal of the European Association of Perinatal Medicine, the Federation of Asia and Oceania Perinatal Societies, the International Society of Perinatal Obstetricians21(9)663-70(2008 Sep)
Chaiworapongsa T,Hong JS,Hull WM,Romero R,Whitsett JA


 
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